LOCUS       DASSLD010000001         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_423544,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000001 DASSLD010000000
VERSION     DASSLD010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423544"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /gene="gatB"
                     /locus_tag="VFK23_00005"
     CDS             complement(<1..732)
                     /gene="gatB"
                     /locus_tag="VFK23_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET7317492.1"
                     /translation="MKYEAVIGLEVHAQLQTNTKIFCGCETRFGEEANTRTCPVCIGM
                     PGVLPVLNKKAIEYIIKTGIAMHCTISPYSRFARKNYFYPDLPKGYQISQYELPVCEH
                     GYVEIVADGKIKRVGITRIHLEEDAGKNLHQAESGASLVDLNRAGTPLMEIVSEPDIR
                     TPEEASEYLKKLRSILRYIEVSDADMEKGNFRCDANVSIRPAGSTEFGTRTEIKNVNS
                     FKFVQKAIEYEIKRQAQVLDEGGRVI"
     gene            complement(788..2248)
                     /gene="gatA"
                     /locus_tag="VFK23_00010"
     CDS             complement(788..2248)
                     /gene="gatA"
                     /locus_tag="VFK23_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET7317493.1"
                     /translation="MELYERTIHELHDMLVKGETTSEAVTESVLDRIAAVDGKVKAYI
                     TVTEDVARALAKEADKKIKAGDTSSPLLGIPVAVKDNMCTEGIRTTCASKILGRFVPP
                     YDATVVRNLKKAGYVLCGKPNMDEFAMGSSTENSGFFVTKNPWDLERIPGGSSGGSAS
                     AVAAGECIGSLGSDTGGSIRQPAACCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITK
                     DVTDSALLMNVIAGHDEKDSTSANIPAPDFTRALRKDVKGMKIGLPKEYFIEGMDSEV
                     ERAVRDAVKTLEGLGAKVVEVTLPHTDYAVATYYILATSEASSNLARYDGVKYGHRTS
                     SPKDLMDMYMKTRDEGFGPEVKRRIMLGTYALSAGYYEAYYKKGQQVRTLIKRDFEEA
                     FKTVDVIATPTAPTAAFKIGEKTTDPLQMYLSDIFTISVNLAGIPGISLPCGFTSANL
                     PIGLQLLGRHFDEESILHAAFAYEQATEWHKRRALL"
BASE COUNT          374 a          739 c          657 g          520 t
ORIGIN      
        1 gatgacccgt ccgccctcgt cgaggacctg cgcctgccgt ttgatctcgt actcgatcgc
       61 cttctgaacg aacttgaagg agtttacatt cttgatctcc gtccgggtgc cgaactccgt
      121 gcttcccgca ggccgtatcg aaacgttggc gtcgcagcgg aaattcccct tctccatgtc
      181 ggcgtcggac acttcgatgt aacgaaggat ggagcggagc ttcttcaggt actccgaggc
      241 ctcttccggt gtgcggatgt ccggctcgga cacgatctcc atgagcggcg tcccggcccg
      301 gttcaggtcc acgaggctcg ccccgctttc cgcctggtgc aggttcttgc ctgcgtcctc
      361 ctcgagatgg atccgcgtga tgccgacgcg cttgattttc ccgtccgcca cgatctcgac
      421 gtacccgtgc tcgcagaccg gcagctcata ttgcgatatc tgatagccct tggggaggtc
      481 gggatagaaa tagtttttcc gcgcaaaccg gctgtagggg ctgatcgtgc agtgcatggc
      541 gatccccgtc ttgatgatgt attcgatcgc ttttttgttc agcacgggca gcactcccgg
      601 catgccgatg cagaccgggc aggtgcgcgt gttggcctcc tcgccgaacc gcgtctcgca
      661 gccgcagaag atcttcgtgt tggtctggag ctgggcatgc acctcaagcc cgatgactgc
      721 ctcgtatttc atcgttgaag ttcctctttt gtttttacat tccgcattcc gaaatccgaa
      781 ttccgcatta gagcaaagcc cgcctcttgt gccactccgt cgcctgctcg taggcgaagg
      841 ccgcgtgcag gatcgactcc tcgtcgaagt gcctgccgag gagttgaagc ccgatgggca
      901 aattcgccga ggtgaacccg cagggaagcg atatgcccgg gatgcccgcc aggttcacgc
      961 tgatggtgaa gatgtccgaa agatacatct ggagcgggtc ggtcgttttc tctccgatct
     1021 tgaacgcggc tgttggcgcc gtcggcgttg cgatcacgtc gaccgtctta aacgcctcct
     1081 cgaagtcgcg cttgatcagc gtacggacct gctgtccctt tttgtaatag gcctcgtaat
     1141 accccgcgga cagcgcgtag gtcccgagca tgatcctgcg cttcacctcg gggccgaaac
     1201 cctcgtcccg cgtcttcatg tacatgtcca tgaggtcttt cggcgaagac gtgcggtgcc
     1261 cgtacttcac gccgtcatac cgcgcgaggt ttgagctcgc ctcggacgtg gcgagaatat
     1321 agtaggtggc aacggcgtag tccgtgtgcg gaagagtgac ctccactacc ttcgcgccga
     1381 gcccctcgag cgttttcacc gcatccctga ccgctctctc cacctcgctg tccatgccct
     1441 cgatgaagta ttccttcggc aggccgatct tcatgccttt cacgtctttc cggagagccc
     1501 gcgtgaaatc gggggccggg atgttcgccg acgtggagtc cttttcgtca tgacccgcaa
     1561 tgacgttcat caggagcgcc gagtcagtca cgtccttggt gatcgggccg atctggtcca
     1621 gcgacgacgc gaacgccacg aggccgtagc gcgacaccct tccgtacgtc ggtttcaggc
     1681 ccacgacgcc gcagcacgcc gccggctggc ggatcgatcc gccggtgtcc gagcccagcg
     1741 acccgatgca ttctcccgcg gccacggccg acgccgagcc gccgctcgat ccgccgggga
     1801 tgcgctccag gtcccacgga ttcttcgtga cgaagaaccc cgagttctcc gtcgaggatc
     1861 ccatggcgaa ctcgtccatg ttgggcttgc cgcagagcac gtagcccgcc tttttcaggt
     1921 tccgcacgac cgtcgcgtcg taggggggga cgaagcgccc gagtatcttc gaagcgcagg
     1981 tggtcctgat cccctcggtg cacatgttgt ccttgaccgc gaccgggatt ccgagaagcg
     2041 gcgacgacgt atctccggcc ttgatcttct tgtccgcttc ctttgccagc gcgcgggcaa
     2101 catcctccgt cacggtgata tacgccttga ccttgccgtc gaccgccgcg atcctgtcga
     2161 ggaccgactc ggttacggcc tccgaggtgg tctcaccttt cacgagcatg tcgtgcagtt
     2221 cgtggatagt ccgctcgtat aactccatta tttctccctc acccggttgt tttcatccac
     2281 cagaacgatt
//
LOCUS       DASSLD010000002         2761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1206362, whole genome shotgun sequence.
ACCESSION   DASSLD010000002 DASSLD010000000
VERSION     DASSLD010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206362"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VFK23_00015"
     CDS             <1..754
                     /locus_tag="VFK23_00015"
                     /inference="COORDINATES: protein motif:HMM:NF037628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HET7317494.1"
                     /translation="QSVEETIWKDARTLCLQADPGTSGALVLASERWHPGVIGIVASR
                     LVEEFYRPTALVCVQNGVGKGSARSIPGFDLYEGLEACSDLLLGFGGHKYAAGFVIAE
                     ENIPRFRDRLGFLVIERMGAGGFTRTLCVDAAVTLDELTVDLMREIEKLAPYGQGNPE
                     PRLGARSLDVLSGRIVGNNHLKVRLRQGNGAALDAIAFNKGALLGKQVKAGARLAAVF
                     TPRLNCWNGSTAGELEIRDLKIDKAEGKRVGR"
     gene            829..>2761
                     /locus_tag="VFK23_00020"
     CDS             829..>2761
                     /locus_tag="VFK23_00020"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET7317495.1"
                     /translation="MPAVRLDDIVERIQSYNPGADVDLLRRAYIFSAKAHQGQTRLSG
                     EAYLIHPIEVAAVLASLKLDVPTVAAGLLHDTIEDTDVTLEEIKSLFGDEVARLVDGM
                     TKLSRMELQSRAEREAENFRKMVVDMAKDIRVILIKLADRLHNMRTLKSLPPEKQKRI
                     SQETLDIYAPLANRLGISKIKTELEDLSFMYLNPEAYRELAQKVVQRRVERETYINEL
                     IEIMKGQLAEHGFKGEVKGRPKHFYSIWQKMQKQGIAFEDIYDLIAIRIITDTKVNCY
                     AILGLIHSLWTPVPGRFKDFIGVPKSNLYQSLHTTVIGPKGERVEFQIRTEEMHRVAE
                     EGIAAHWRYKERSAISPREEQQFAWLRQLMEWQRDLTDAKEFMDTVKGDLFPDVVYVF
                     TPRGDVKELPQGSTPIDFAYSVHTDIGHQCVGAKVNGKIVPLKHILRNGDKIEVITQA
                     GHTPSRDWLKFVKTSKAKTRIKAWLKIEERRRSILLGKELLEKDLRKHELSPAKVLKS
                     DELLQIANEMSHAALDDLFAAIGYGKVSAHMVANKLAPDNPHVEPVPKRAPQRQGKPS
                     VGGMKISGMDNMLIHLSKCCSPVPGDKVIGFITRGRGVSIHTADCPNVAELTFDKDRL
                     IEVSWGDFKPGAHSVKIAVH"
BASE COUNT          621 a          825 c          834 g          481 t
ORIGIN      
        1 acaatctgtc gaagaaacca tatggaagga cgcacgcacg ctctgcctgc aggctgaccc
       61 cggcacctcc ggggcgctgg tgctggcatc ggaacggtgg catccggggg tcatcggcat
      121 tgtggcgtcc cgtctcgtcg aagagttcta caggcccacg gcgctcgtat gcgttcagaa
      181 cggtgtggga aagggttccg ccagaagcat ccccggcttc gacctctacg agggccttga
      241 ggcctgttcc gacctgcttc tggggttcgg cggccataaa tatgccgcgg ggttcgtcat
      301 tgcggaggaa aatatccccc ggttccgcga ccggctgggt tttctggtca tcgagaggat
      361 gggggccggg ggcttcaccc ggacattgtg cgtggacgcg gctgtgacgc tcgatgaact
      421 gacggtcgac ctcatgcggg agatcgagaa actggcgccc tacggccagg gaaatccgga
      481 gccgcggctc ggggcgcgga gtctcgatgt gctctccgga aggatcgtgg gaaacaatca
      541 cctgaaggta agactgaggc agggaaacgg cgcggcgctc gacgcgatcg ccttcaacaa
      601 gggcgcgctc ctcggaaaac aggtgaaggc gggggcgcgc ctcgccgcgg tattcacccc
      661 gcgtctcaac tgctggaacg gttcgacggc cggggaactg gagatacggg acttgaaaat
      721 cgataaagca gagggtaaga gggtaggaag gtaggacgtt tagtccttaa gccgtttctt
      781 accctcccac cttcttacct tcccaacttc tgcctttgaa ggtagcctat gcctgcggtt
      841 cgcctcgatg acatagttga gcgcatccaa agctacaacc ccggcgcgga cgtcgacctg
      901 ctgaggcggg catatatctt ttccgccaag gcacaccagg gacagacgcg tctttcgggc
      961 gaggcctacc tgatccatcc gatcgaggtg gcggccgtgc tcgcaagcct caaactcgac
     1021 gtgcccaccg tggcggccgg cctccttcac gacacgatcg aggacaccga tgtaacgctg
     1081 gaggagatca aatccctgtt cggggacgag gttgccaggc tggtcgacgg catgacgaag
     1141 ctgtcccgca tggagctcca aagccgtgcg gagcgcgaag cagagaattt ccgcaagatg
     1201 gtcgtggata tggccaagga catcagggtc atcctgatca agcttgccga ccggctccac
     1261 aacatgcgca ccctcaagtc cctcccgccc gagaaacaga aacgcatatc ccaggaaacg
     1321 ctcgacatct acgcccccct cgccaaccgc ctcggcattt cgaagatcaa gaccgaactg
     1381 gaagacctct ccttcatgta cctgaaccct gaggcctacc gcgaactcgc acagaaggtc
     1441 gtgcagcgcc gcgtcgaacg cgagacctat atcaacgaac tcatagaaat catgaagggc
     1501 cagctcgcgg aacacgggtt caagggggag gtgaagggca gacccaagca cttctacagc
     1561 atctggcaga agatgcagaa gcaggggata gcctttgagg acatctacga cctcatcgcg
     1621 atccggatca tcaccgacac caaggtgaat tgttacgcca tcctggggct catccactcg
     1681 ctctggacgc cggtcccggg caggttcaag gattttatcg gcgtgccgaa atccaacctt
     1741 tatcaatcgc tccacaccac cgtcatcggc cccaagggcg agcgggtaga attccagatc
     1801 cggaccgaag aaatgcaccg cgtggcagag gaaggcattg ccgcgcattg gcggtacaag
     1861 gagcgctccg cgatcagccc gcgggaggag cagcagttcg cctggctccg gcagctgatg
     1921 gagtggcagc gcgacctgac ggacgccaag gagttcatgg acacggtcaa gggagacctt
     1981 ttcccggacg tggtctacgt cttcacgccg cgcggcgacg tcaaggaact tccgcagggc
     2041 tcgacgccca tcgattttgc ctacagcgtc cataccgata taggccacca gtgcgtgggc
     2101 gccaaggtga acggcaagat cgtccccctc aagcacatcc ttcgcaacgg cgacaagatc
     2161 gaggtaataa cccaggccgg ccacacgccg agccgggatt ggctcaagtt cgtcaagacc
     2221 tcgaaggcga agaccaggat caaggcatgg ctcaagatcg aggagcgacg gcgaagcatc
     2281 ctgctgggca aagaactcct cgaaaaggac ctgcggaagc atgaactgag cccggcgaag
     2341 gttctcaaat cggatgaatt gctccagata gcgaacgaaa tgtcccatgc cgccctcgac
     2401 gacctgttcg ctgcgatagg ctacggaaag gtctcggcac atatggttgc gaacaagctt
     2461 gctcccgaca acccgcatgt ggaaccggtt ccgaaacggg ctcctcagag gcagggaaaa
     2521 ccgtcagtcg ggggcatgaa aatcagcggt atggacaata tgctgatcca cctttccaag
     2581 tgctgcagcc ccgtgccggg cgataaggta atcgggttca tcacgcgggg caggggcgtg
     2641 tccatccata ccgcggactg ccccaacgtc gccgagctta ccttcgacaa ggaccgtctt
     2701 atcgaagtct cgtggggcga cttcaagccc ggcgctcatt ccgtgaagat agccgtccat
     2761 a
//
LOCUS       DASSLD010000003         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1531809, whole genome shotgun sequence.
ACCESSION   DASSLD010000003 DASSLD010000000
VERSION     DASSLD010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531809"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFK23_00025"
     CDS             complement(<1..254)
                     /locus_tag="VFK23_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET7317496.1"
                     /translation="MNVNTILFDNRNSSYIVVLKDESGSKILPIWIGAAEGNSIAMAM
                     SNIKAARPLTHDLIVSIFDRLEIEIARVVISDLIDNTFYAS"
     gene            398..2062
                     /gene="argS"
                     /locus_tag="VFK23_00030"
     CDS             398..2062
                     /gene="argS"
                     /locus_tag="VFK23_00030"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET7317497.1"
                     /translation="MKQTLTEILLQALRRAKEKGELKLESQPVITLDAPREKTHGDLA
                     TTVAMALAKPEAKPPRKIAEIIVGNIRDDEGIIEKTEIAGPGFINFFFRQDRWKKTLF
                     EIDAEGAAFGVPDIGKGEKVLVEFVSANPTGPLHVGHGRGAAVGDALANLLSAAGFEV
                     SREFYINDAGRQIRLLALSVYARYQQSFGNDVPFPEDGYHGGYIEEIAQGFAKVHGDR
                     FRSIPFDDCAAHFAEYGKNAMLADIRTDLETFGVRFDTWFSEQSLLTEGSVQRSIEEL
                     KERGFAYEKDGALWLKSTAFGDDKDRVIIKKDGEYTYLATDIAYHRNKLARGFQQLVN
                     IWGADHHGYIPRVQAVIQAFGHPKDSLHVLLVQLVAILRHGQPVPMSKRAGTFVTLRD
                     VVQDVGADAARYIFLTRRSDSHLDFDLDVAKEQSRENPVYYVQYAHARLASLFREAEA
                     RNIAFPQSGAVDLALLDLEEDQNIIKALAKYPEVIEEAALAYEPHRLTFYLQELAGLL
                     HNYYFKHRIITEDHARTGARLFMMKQVKSVIRSALSILGVTAPERM"
BASE COUNT          465 a          591 c          609 g          398 t
ORIGIN      
        1 gatgcgtaaa aggtgttgtc gatcaggtcg ctgatgacca cgcgggcgat ttctatttca
       61 agccggtcga agatgctgac gatcaggtca tgggtgaggg ggcgtgcggc cttgatgttg
      121 ctcatggcca tggcgatgga attgccttcc gcggccccga tccagatggg aagtatcttg
      181 ctgccgctct catccttcaa aaccacgata tagctgctgt tcctgttgtc gaacaggatc
      241 gtatttacat tcatttggac ccacataccg gacccctcta tctttcttaa acgataccac
      301 accgtagaaa gtaagtaaag ggattatttt attgactttt ttcagagata cgatatactt
      361 ccagacttgt aacccgcgaa agaaacactg attatcgatg aaacaaacac tgaccgaaat
      421 cctgcttcag gccctgcgcc gggccaaaga aaaaggcgaa ctgaagcttg aatcacagcc
      481 tgtaatcacg ctcgatgcac cgcgggagaa gacgcacggt gaccttgcca ccacggtggc
      541 catggcactc gctaaaccgg aggccaagcc gccccggaag atcgccgaga tcatagtcgg
      601 caatatccgg gatgacgagg gcatcatcga aaagaccgag atcgcaggcc ccggtttcat
      661 caattttttt ttccggcagg accggtggaa aaagacgctc tttgaaatag acgcggaggg
      721 ggcagccttc ggggtgcctg acatcggcaa aggcgagaaa gtcctcgtcg aattcgtgag
      781 cgcgaacccc acgggaccgc tccatgtggg ccacggccgg ggcgctgcgg tcggcgacgc
      841 gctggcgaac ctcctctccg cggcagggtt cgaggtcagc cgcgagtttt acatcaacga
      901 cgcgggaagg cagatacgcc tgctcgcgct gtcggtatac gcccggtacc agcagtcgtt
      961 cggaaacgac gtgccctttc ccgaggacgg ctaccacggc ggctacatcg aggaaatcgc
     1021 ccagggtttc gccaaggtcc acggtgaccg cttccggagc ataccgttcg atgactgcgc
     1081 ggctcatttc gcggaatacg gcaaaaatgc catgctggcc gacatcagga cggacctgga
     1141 gaccttcggt gtccggttcg acacgtggtt cagcgagcag agcctgctga ccgagggatc
     1201 cgtccagcgc tcgatcgagg aattgaagga gcgcggtttc gcgtacgaaa aggacggcgc
     1261 gctctggctg aagtcaactg catttggcga tgataaagac cgggtaatca tcaagaagga
     1321 cggcgagtac acctatctcg ccaccgacat cgcttatcac cggaacaagc tcgcccgggg
     1381 atttcagcag ctcgtcaaca tctggggcgc ggaccatcac ggttacatcc cccgcgtgca
     1441 ggccgtgatc caggcattcg ggcacccgaa agactcgctc cacgtgcttc tcgtgcagct
     1501 cgtggcgatc ctgcggcacg ggcagcccgt cccgatgtcc aagcgggcgg ggaccttcgt
     1561 aacgctccgg gacgtcgtcc aggatgtcgg cgcggacgcg gcgcgctata tattcctgac
     1621 gcgccggtcc gacagccatc ttgatttcga ccttgacgtg gcaaaggaac agtcccggga
     1681 aaacccggtg tactacgttc agtatgcgca cgcccggctt gcaagcctgt tccgcgaggc
     1741 ggaggcgaga aatatcgcct ttccgcaaag cggtgccgtc gaccttgcgc tgctcgacct
     1801 cgaggaagac cagaacatca tcaaggcgct ggcaaaatat cccgaagtaa tcgaagaggc
     1861 ggcgctggcg tacgaaccgc accggctgac gttctatctc caggaactcg ccgggctgct
     1921 tcataactac tacttcaaac accggatcat cactgaggac catgcgcgta cgggggcaag
     1981 gctcttcatg atgaaacagg tgaagtcggt gatccggagc gcgctctcga tattgggcgt
     2041 gacggcgccg gagaggatgt gag
//
LOCUS       DASSLD010000004         3564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1238628, whole genome shotgun sequence.
ACCESSION   DASSLD010000004 DASSLD010000000
VERSION     DASSLD010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238628"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VFK23_00035"
     CDS             <1..903
                     /locus_tag="VFK23_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7317498.1"
                     /translation="AGTVTRSIHELQKGAEAVAAGNLGHRVGTAARDEIGSLSRSFDA
                     MTEALHRDIARREKAESDIRSLNDELQHHVRQLEESNRELEAFSYSVSHDLRSPLRSI
                     DGFSLALIEDYADKLDDEGKEYLARVRAATQRMGQLIDDLLKLSRLARMEINREQVDL
                     SALAASIASRLRQNHPEREAEFIIAGGLTGLGDERLLTAALENLFGNAWKFSEKTPRT
                     VIEFGVTDAGGDPAFFVKDNGAGFDMAYAHKLFSPFQRLHHAAEFSGTGIGLATVKRI
                     IDRHGGRVWIEGAVGKGTTVYFTL"
     gene            1039..1470
                     /locus_tag="VFK23_00040"
     CDS             1039..1470
                     /locus_tag="VFK23_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7317499.1"
                     /translation="MKSKIILLVEDNPDDVKLTERALKKSKILNELMVAQDGVEAVDY
                     LFGTGKYAGRDTKALPQVVLLDLKMPRMDGLEVLQRIRTDERTKLLPVVVLTTSSEDR
                     DRVESYKLGANSYIRKPVDFDQFVSAVQNLGLYWLVLNEAP"
     gene            1538..>3564
                     /locus_tag="VFK23_00045"
     CDS             1538..>3564
                     /locus_tag="VFK23_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7317500.1"
                     /translation="MSKPLNILIVEDSEDDALLLLRELRRGGYEPVSERVETREDMKA
                     ALDRRQWDIIISDYMMPKFSGLAALEVLKESGADLPFIIVSGNIGEDIAVEAMKAGAH
                     DYIIKGKLTRLVPAVDRELREAADRSERKRTEEAFRIEQTFRRTIEDSILVGIAAVDL
                     SGRLFYVNQALCDMTGWSRQELEGSAPPYAYWPPESVDDITRLQDMAVGGMILKGGHE
                     IRLMRKGGERFDTLCFVSPLLDNEGKPSAWLSTYHDITERKKAARRTEVTNALLKLFT
                     QKFTRKEYLDAACGVLRTWTGCHHAGIRIADRNHNIPFESSEGYDEAFLHEERTLSLV
                     KDQCICTRVIAGTPVAPDLCAMTAEGSFYSNDTDGFAGALTAVERQRYKDVCMRMGYR
                     SLAVIPIRYRETPLGAIHIADEQPDMLPRESVEFLEQLAFIIGEAIFRFGIEEELRNN
                     YDDLQKTTELLERIFSTTHMLVAYLDRDFNFIRVNRAYATDGERSPEFYPGKNLFTLC
                     PDEEMREIFQNVVKTGEPHFVFESAFKYNFKDERKNMYWDWSLLPVREADGKIVGLLF
                     TLLDVTARKKAEQELRRANAYNRSLIEASLDPLVTIGPDGKITDVNAATEAATGRSRQ
                     DLIGTDFSEYFTEPDRARAGYLKVFREGSIRDYALDIRRGDGCATPALYNAS"
BASE COUNT          765 a         1100 c         1096 g          603 t
ORIGIN      
        1 gccggaacgg tcacgcgctc gatccatgaa ctgcagaaag gcgccgaggc cgttgctgcc
       61 ggcaacctgg gccaccgggt cggaacagcc gcccgtgacg agatcggcag cctctcccgg
      121 tccttcgacg cgatgacgga agcgctccac cgcgatatcg cgcgccggga aaaggccgag
      181 agcgatatcc ggtcgctgaa cgatgagttg cagcaccatg tccgccagct cgaggagtcc
      241 aaccgggagc tggaggcgtt cagctattcc gtttcccacg atcttcggtc gccgctccgg
      301 agcatcgacg gcttcagcct ggccctcatc gaggattatg ccgacaagct ggacgacgaa
      361 ggcaaggaat accttgctcg cgtccgcgcc gcgacccagc gcatggggca gctcatcgac
      421 gacctgctga agctgtcgcg cctggcccgg atggagatta accgcgaaca ggtggacctg
      481 agcgccctgg cggccagtat cgcctcgcga ctccggcaaa accatccgga gcgggaggcg
      541 gaattcatta ttgcgggcgg gctcacgggc ctgggcgacg aacggctcct gaccgcggcc
      601 ctggaaaacc ttttcggcaa tgcatggaaa tttagtgaaa agaccccccg cactgtgata
      661 gaattcgggg taaccgacgc cgggggcgat cctgcgtttt tcgtgaaaga caacggcgcc
      721 ggtttcgaca tggcctatgc ccacaagctg ttcagcccct tccagcggct tcatcacgcg
      781 gccgaatttt ccggcaccgg gatcggcctt gcaacggtca agcgcatcat cgaccgccac
      841 ggaggccggg tctggatcga aggggcggtg ggcaaaggaa caacggtgta tttcactctg
      901 taatgacagc aacaatttta ccgcagagac gcaaagacgc agagaaaaac caggagttgg
      961 ggttgactca cgattttaaa agctttatct ccgtgtctca gcgcctccgc ggtaaataat
     1021 ttctttccag gagcgcctat gaaaagtaag attatcctgc tggtcgaaga caaccccgat
     1081 gacgtgaaac tgacggagcg ggcgctcaag aaaagcaaga ttctgaacga actgatggtg
     1141 gcgcaggacg gagtggaagc ggtggactac ctgttcggca ccggcaagta cgccggccgg
     1201 gataccaaag ctctgccgca ggtggtcctg ctggacctca agatgccacg gatggacggg
     1261 ctcgaagtcc tgcagcgcat ccgcaccgat gaacggacga agcttctgcc cgtggtggtc
     1321 ctcaccacgt cgagcgagga ccgggaccgc gtcgaaagct acaagctggg ggccaacagc
     1381 tacatccgca agcccgtgga cttcgaccag tttgtgagcg ccgtgcagaa ccttgggctg
     1441 tactggctgg tattgaatga agcgccgtga aattttgtag ccgcaacctt caggttacgc
     1501 ttttttccgc aggctgaagc ctgcggctac cgtatttatg tccaaacctc tcaacattct
     1561 catcgtcgaa gactccgaag acgacgccct cctgctgctc cgggaactga ggcggggcgg
     1621 ctatgagccc gtgtccgagc gcgtcgagac gcgcgaagac atgaaggccg cccttgaccg
     1681 ccggcagtgg gacatcatca tctcggacta catgatgccg aagttcagcg gcctggcagc
     1741 cctcgaggtc ctcaaggaaa gcggggccga cctgccgttc atcatcgtct cgggcaacat
     1801 cggcgaggac atcgccgtgg aggccatgaa ggcgggggcg cacgattata tcatcaaggg
     1861 caaactcacc aggctcgtcc cggccgtgga ccgggaactg cgggaggcgg cagaccgcag
     1921 cgagcgcaag cggaccgagg aagcgttccg gatcgagcag actttccgcc ggacgatcga
     1981 ggactccatc ctcgtcggca tagccgccgt cgacctttcc ggcagactgt tctacgtgaa
     2041 tcaggcgctg tgcgacatga cgggctggag caggcaggag ctcgaagggt ccgcgccgcc
     2101 ctatgcctat tggccccccg agtccgttga cgatatcacg aggctccagg acatggctgt
     2161 cggagggatg atcctgaagg gcggccacga aatccggctc atgcggaagg gcggggagcg
     2221 gttcgatacc ttgtgcttcg tctcgcccct cctggacaat gaaggaaagc cgtcggcgtg
     2281 gctctccacc taccacgaca tcacggagcg caagaaggcg gcgcggcgca ccgaagtgac
     2341 caacgccctg ctcaaactct tcacccagaa atttacgcgc aaagagtacc tcgacgcggc
     2401 ctgcggcgtg ctccgaacct ggaccggctg ccaccatgcg ggcatccgga tcgcggaccg
     2461 caaccacaac atccccttcg agtccagcga aggctatgac gaggcgttcc tgcacgagga
     2521 gcgcacgctg tctctcgtca aggaccagtg catctgcacc cgggtcatcg cggggacccc
     2581 ggtggcaccc gacctctgcg ccatgacggc cgagggctcg ttctactcca acgatacgga
     2641 cggcttcgcc ggcgctctca ccgcagtgga gaggcagcgg tacaaggacg tctgcatgcg
     2701 aatgggatac agatcgctgg ccgtcattcc gatccgctac cgggaaaccc ccctcggggc
     2761 gattcacatc gccgacgaac agccggacat gcttccccgg gagagcgtgg agttcctgga
     2821 acagcttgcg ttcatcatcg gcgaggcaat attccggttc ggcatcgagg aagagctccg
     2881 gaacaattac gacgacctcc agaagacgac agagctcctc gagaggatat tctccacgac
     2941 ccacatgctc gtggcctacc tggaccggga cttcaacttc attcgcgtaa accgcgccta
     3001 cgccacggac ggcgaacgga gccccgagtt ctatcccgga aagaaccttt tcacgctctg
     3061 ccccgacgag gaaatgcggg agattttcca aaatgtcgtg aagacgggag aaccccattt
     3121 cgtctttgag agcgccttca agtacaactt caaggatgag cggaagaaca tgtactggga
     3181 ctggagcctg ctcccggtca gggaggccga cgggaagatc gtgggcctgc tcttcacgct
     3241 cctcgacgtc accgcccgga aaaaggccga acaggaactg aggcgtgcaa acgcttacaa
     3301 ccggagcctg atcgaggcca gtctggatcc gctggtgacc atcggtcccg acgggaagat
     3361 caccgacgtc aacgccgcca cggaagcggc gacaggccgg tcgcggcagg acctcatcgg
     3421 caccgacttc tccgagtatt tcactgagcc ggacagggcg cgagccgggt acctgaaggt
     3481 cttccgcgaa ggctcgatca gggactacgc cctggatatc cggcgcggcg acggctgcgc
     3541 gacgccggct ctgtacaacg cgtc
//
LOCUS       DASSLD010000005         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_585477,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000005 DASSLD010000000
VERSION     DASSLD010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585477"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VFK23_00050"
     CDS             <1..466
                     /locus_tag="VFK23_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317501.1"
                     /translation="QEGVGGYQVVGDAILYDRSAWSNIVSASSPTFTIAQLSVQSPWT
                     GNTISGSITMNNAIKMNGVMNRGFLFVAHGGMIVSAINVSAQMDSGGAYNVPDIPGGT
                     AAQPLPLAFYGVEAVGWQALFPRLHKAIAVPAIADVRAGDATGVDMNMLPLW"
     gene            603..1232
                     /locus_tag="VFK23_00055"
     CDS             603..1232
                     /locus_tag="VFK23_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HET7317502.1"
                     /translation="MKKIRGLLKKYFITGLLVILPIWATYYVLTALLGVIDGLLADLP
                     AQFFGYRIPGMGIITLILVVLLVGMFSANYLGGRIVRTSDELLHKVPLVRGVYFTVKQ
                     VMETFSMKHQFRGVALVEYPRKGCYSVGFMTGEVQGGTVGMSGKFVTVFVPTTPNPTA
                     GFLLILPESEVAKLDMSVEEGMKFIISLGLVPLNEQQTHKLKQMAQEVR"
     gene            1233..>2246
                     /gene="glmU"
                     /locus_tag="VFK23_00060"
     CDS             1233..>2246
                     /gene="glmU"
                     /locus_tag="VFK23_00060"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET7317503.1"
                     /translation="MKPTVVILAAGLGTRMKSGLAKALHPLAGMPLVQHVLNTAADLD
                     PEKTILVLGHQAGKVRDAIFGTRVEIVLQAEQLGTGHAVLQAREAIFGVSGPVVILCA
                     DTPLLTGGTLRSALELHRKSRAAVTLITALLADPHGYGRVVRKGTGVMRVVEEKDATT
                     AQKKIREVNAGIYCFDREFLLASLNTLGRDNAQGEYYLPDTIELAKKKRRTVAALVCG
                     DPDEVMGVNSRYDLSRAEAVMRRRINRRWMAEGVTMFDPDTVFVGTEASLGRDVVLYP
                     NVRIEGKTKLGDACTVYPGSRIVDSTVENGVTIKDCSVIEESDIATGAQIGPFAHLRP
                     GSVI"
BASE COUNT          444 a          679 c          695 g          428 t
ORIGIN      
        1 ccaggaaggc gtcggaggat atcaggtcgt cggcgacgcg atactgtacg accggagcgc
       61 ttggtcgaac atcgtttcgg cttcatcgcc aacctttacc attgcgcagc tgtcggtgca
      121 gtcgccttgg accggcaaca cgatctcagg ttctatcacc atgaacaacg ccatcaagat
      181 gaacggcgtc atgaacaggg ggttcctgtt cgtggcccat ggcgggatga tcgtgagtgc
      241 tataaacgtc agtgctcaga tggactcggg cggggcctat aacgtgccgg atataccggg
      301 cgggaccgcg gcgcagccgc ttccccttgc gttttacggt gtcgaggccg tgggctggca
      361 ggctctcttt ccccggctgc ataaggcgat tgcggtcccc gcgattgccg atgtccgcgc
      421 gggcgatgcc acgggtgtcg atatgaacat gctgccgctc tggtagcccg cgccgatgct
      481 cctctctaga aggccttccg gaccccggag ggcctttttt ttcaggcgag cacgacggtt
      541 tcctcatgcc gcgccggaaa aaaagcttgc aacgtcagcg taaaatcagt aatattacaa
      601 acgtgaaaaa aatcaggggt ttgctaaaga aatattttat caccggactg ctcgtaatcc
      661 tgcctatctg ggccacgtac tatgtcctga ccgcgctcct gggcgtaatc gacgggcttc
      721 tggcagacct tccggcgcag tttttcgggt accggattcc cgggatgggc atcatcacac
      781 tgatcctcgt ggttttgctc gtgggcatgt tttccgcgaa ctacctgggc ggccgcatcg
      841 tcagaacgag cgacgagctc ctgcacaagg tcccgctcgt gcgcggggtc tatttcaccg
      901 tgaagcaggt catggaaacc ttctccatga aacaccagtt tcgcggcgtg gccctggtcg
      961 agtatccgcg gaaaggctgt tacagcgtcg gcttcatgac cggcgaggtg cagggaggca
     1021 ccgtcggcat gtccggcaag ttcgtgacgg tcttcgttcc caccacgccg aatcccacgg
     1081 caggcttcct cctcatcctg cccgaatcgg aggtcgccaa gctcgacatg tccgtggaag
     1141 aggggatgaa attcatcata tcgctgggcc tggtccccct gaacgaacag cagactcaca
     1201 agttgaaaca gatggcgcag gaggtccggt aagtgaaacc gaccgtcgtc atccttgcgg
     1261 ccggtctcgg cacccgcatg aagtcggggc ttgccaaggc cctccatccg cttgcgggca
     1321 tgccgctggt ccaacacgtc ctgaacaccg cggcggacct cgatcccgaa aagaccatac
     1381 tcgttctggg acaccaggcg ggcaaggtgc gggacgcgat cttcgggacg cgtgttgaaa
     1441 tcgtgcttca ggccgaacag ctcggtacgg gacacgccgt cctgcaggcg agagaggcca
     1501 ttttcggagt gtccggaccc gtcgtgatcc tgtgcgcgga tacgccgctc ctcacgggcg
     1561 ggacgctccg ctccgcgctg gaacttcacc ggaagtcccg cgccgcggtt acgctcatca
     1621 ccgccctcct cgcggaccca cacggctacg ggagggtcgt gcgcaaaggg accggcgtca
     1681 tgcgcgtcgt ggaggagaag gacgccacca cggcccagaa gaagatccgg gaagtgaacg
     1741 cgggtatcta ctgtttcgat cgggagttcc tcctcgcttc ccttaacacg ctcggcaggg
     1801 acaacgctca gggggaatac tacctgcccg acacgatcga actggcaaaa aagaaaagac
     1861 ggaccgtcgc cgcgctggtg tgcggcgatc ccgacgaggt catgggcgtg aattcgcgat
     1921 atgacctctc ccgggcagag gccgtcatga gacggcggat caaccggcgc tggatggccg
     1981 agggcgtgac catgttcgac cccgacaccg tcttcgtggg gacggaggcc tcgctcggcc
     2041 gggacgtggt gctctacccc aacgtccgga tcgagggaaa aacgaaactt ggcgacgcct
     2101 gtacagtgta tcccggctcg cggatagtag acagcacggt cgagaacggc gtgaccatta
     2161 aggactgctc ggtaatcgaa gaaagcgaca tcgcaaccgg cgcgcagatc gggcccttcg
     2221 cgcatctcag gccggggtcc gtcatc
//
LOCUS       DASSLD010000006         2404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_194728,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000006 DASSLD010000000
VERSION     DASSLD010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194728"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..143
                     /locus_tag="VFK23_00065"
     CDS             <1..143
                     /locus_tag="VFK23_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008683311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HET7317504.1"
                     /translation="IDGGTYGVCESCGGEINIKRLEARPVTTLCIECKTAQEEEEKRQ
                     MD"
     gene            complement(244..1374)
                     /locus_tag="VFK23_00070"
     CDS             complement(244..1374)
                     /locus_tag="VFK23_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317505.1"
                     /translation="MRPLKPFREWGIVLLSFLAFSACHGGGGGAAPAPIPTTVLTVGA
                     PVSDTIVRSTFNAYSATVTPGSFYKISITGMTDDVDLNVYDTDNTFRNPIICPIDNGA
                     IIGTSPEDCILIPSGNTLYFGVNGSVLAGSAAVYTIDVELLPETNLNLSIPQGDQTTQ
                     RTAAAYSLPVTPGAYTVSMTGLTDDADIHVFTENGSVVSPAVCSPPDNTLFIGKEPED
                     CTVNVNTSNTTVLFIVDGIFSSAPTVQYTALVTPAPNVAIPANEGSVAAPINILVDTP
                     TTGSVGPAGTSRYQAAGLLPNLRYTLSITGLTGVANLTPFADSTFTTHIVCSIDNTLL
                     AGTTPKSCTFQAPQAGTVFFDVSSIPGTVNGASYIILVEQGP"
     gene            complement(1398..1799)
                     /locus_tag="VFK23_00075"
     CDS             complement(1398..1799)
                     /locus_tag="VFK23_00075"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET7317506.1"
                     /translation="MSKAKKVKQIQFMMSDQPGRLSEITAALAAAKVNINNICAYGMQ
                     QEATFMLTVDSNAKAKKALAPLGVEIKEQDVVIVEMPNKPGELQKVAKKIGDAGINII
                     YMYGTSAGARSGNCIFATSDDAKAIKLINKE"
     gene            complement(2065..>2404)
                     /locus_tag="VFK23_00080"
     CDS             complement(2065..>2404)
                     /locus_tag="VFK23_00080"
                     /inference="COORDINATES: protein motif:HMM:NF021009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="H-type lectin domain-containing protein"
                     /protein_id="HET7317507.1"
                     /translation="HYSFLLLIVLIGCAPKQLIEIGSVAMPHMKRPTTEQWQMVYKER
                     VTFHQPFRTTPQIQVSLSSIEAYHHTRGTFYQLKTVNPDANGFELQIYGAYMDSFSNC
                     VASWIAVGVR"
BASE COUNT          567 a          608 c          690 g          539 t
ORIGIN      
        1 ggatcgacgg cggcacctac ggcgtctgcg agtcctgcgg cggagagatc aacatcaaga
       61 gactcgaggc gcgtcccgtg acgacgctct gcatcgagtg caaaaccgcc caggaagaag
      121 aagagaagcg gcagatggac tgaccgttct tttctggcgg gaacgaaatc cataatgcaa
      181 ggccgacctt tcagaaggtc ggcctttttg tgttttcggg tgaccgatac cgagcgctct
      241 ctatcaaggg ccttgctcaa ccaggattat atacgaagcg ccgtttacag ttcctgggat
      301 cgatgatacg tcgaagaaga ctgttcctgc ctgcggcgcc tggaaggtgc agctcttcgg
      361 cgtcgtgccg gccaggagag tattatcgat cgagcaaaca atatgggtcg taaaggtgct
      421 gtctgcgaaa ggggtgaggt tcgcgacacc ggtgagcccg gtgatgctaa gagtgtagcg
      481 caaattagga agcagaccgg ccgcctgata tcggcttgtg ccggccgggc ccaccgaccc
      541 ggtagtcggc gtatcgacca atatgtttat gggtgccgcg acggaaccct catttgccgg
      601 aatggccacg ttcggagcag gcgtcacgag ggcagtatac tggaccgtag gagcggagct
      661 gaaaattccg tcgacgatga acaatacggt cgtgttactc gtgttcacat tgacagtaca
      721 gtcctccggc tcctttccga tgaacagggt gttgtccggc ggcgagcaga cagcaggtga
      781 aacgacggaa ccattctcag taaagacatg gatgtccgcg tcatcggtga gcccggtcat
      841 gctgacggtg taagcgccgg gcgtaacggg cagcgaatag gctgccgcgg tccgctgcgt
      901 ggtctggtca ccctggggaa tggagagatt cagattcgtc tcaggcagga gttccacgtc
      961 gatcgtatac acggcggccg atcccgcaag aactgaaccg ttcacgccga agtagagcgt
     1021 atttccggaa ggtatcagga tgcagtcctc gggagaggta ccgatgatag ctccattgtc
     1081 gataggacag atgatcgggt tcctgaaggt gttgtccgta tcgtacacat tgaggtcaac
     1141 atcgtcagtc atgccggtga tgctgatttt atagaaggag ccaggggtaa ccgtcgcgga
     1201 atacgcgtta aaggtcgagc ggactatcgt atcggacacc ggagctccaa cagtaaggac
     1261 cgtcgtcgga atcggcgcag gcgccgcccc tccgccgccc ccgtggcaag ccgaaaacgc
     1321 cagaaaagaa agcagcacaa taccccattc cctgaacggc ttcaacggtc tcatcgctca
     1381 tcctcccgga atcatgttca ctccttattg atcagcttga tggccttggc gtcgtcagag
     1441 gtggcaaaaa tacagttgcc cgacctcgcg ccggcagagg tgccgtacat atagatgatg
     1501 ttgattcccg cgtcgccgat tttcttagcc actttctgga gctcccctgg tttgttcggc
     1561 atctccacga tgacgacgtc ctgttccttg atctcgacgc ccaggggcgc aagcgccttc
     1621 tttgctttcg cgttgctgtc cacggtcagc atgaaggtcg cttcctgctg catgccgtag
     1681 gcgcagatgt tgttgatgtt caccttggca gcagcgagtg ctgccgtgat ctctgaaagc
     1741 ctgcccggtt ggtcggacat catgaactgg atctgtttta ccttttttgc ctttgacatg
     1801 gctgatcctc ctgaagtgaa tcataagtat ttagtcgcat gggtacaaat caacttcccg
     1861 cttcatgcat cacctgcttc cagtatatca ggagtcgtgc cgcgattgga ggaagggagg
     1921 gagaaaaggc gaagagatga gcctaaaacg gaatactcgg cgttcgactt ccacgaacaa
     1981 atataatagg gaggtcagcg gtcaagagcc tggggaaaat gccaagactc ctcggttact
     2041 gtcttttttt taaaccaagc agttctatcg aacaccgacg gcaatccaac tggcgacaca
     2101 gttcgaaaaa gaatccatgt acgcaccgta aatttgtaat tcgaatccgt ttgcgtcagg
     2161 attgacagtt ttcagctgat aaaatgttcc tcgggtgtga tgatacgcct caatagaact
     2221 gagtgagact tgaatttgcg gcgtggtgcg gaagggctga tgaaatgtaa cgcgctcttt
     2281 gtaaaccatc tgccactgtt ctgtagtggg acgtttcatg tgaggcatag caacagaacc
     2341 gatctcgatg agttgcttag gcgcacaacc aataagaaca atgagcaaaa gaaaagaata
     2401 atgc
//
LOCUS       DASSLD010000007         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1466844, whole genome shotgun sequence.
ACCESSION   DASSLD010000007 DASSLD010000000
VERSION     DASSLD010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466844"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..2022
                     /locus_tag="VFK23_00085"
     CDS             124..2022
                     /locus_tag="VFK23_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7317508.1"
                     /translation="MAIVSLQGVNVGFGGPLLLEGVYLSIDRGERVCLVGRNGTGKST
                     ILRLITGEVKPDAGKIVFQQSVRVTLLTQEVPEALSGSVFDVVSGAFGEQGKLLAEYH
                     HLSGSLVHDHSGKLMARLEEVQHRFEAAGGWQMQQKVEEILTRLRLPEDAEFSELSGG
                     LKRRVLLAKALAGEPDLLLLDEPTNHLDIDSIAWLEEFLLAFGGTLLFVTHDQMLIQK
                     LATRILDLDRGRLTSWPGNYQTYLERKQAALDAEAVENAKFDKKLAAEEAWIRQGVKA
                     RRTRNEGRVRALKELRRIRSERREVAGAARMQMQEAERTGKLVIEATDVRYDYSGRPV
                     IRDFSTTIIRGDKIGIIGPNGSGKTTLLKLLLGELKPQTGKIKHGTNLEVAYLDQHRA
                     QLDDEKTVQDNVANDSDYVTINGNRRHVIGYLGDFLFAPARARSPVKVLSGGERNRLL
                     LAKLFTKPSNVLVLDEPTNDLDLETLDLLEELLMDYPGTVLLVSHDRAFINNVVTSTL
                     VLEGEGMVGEYVGGYDDWMLQSRRMTSETAVPVKPEAKKEPARPQKERPRKLTFKEQK
                     ELDALPKRIEALEAEQQQIQKDLADPAFYRESGNKVSEYKTRLETLEKELTEAYTRWE
                     ELEALSVK"
     gene            complement(2264..>2656)
                     /locus_tag="VFK23_00090"
     CDS             complement(2264..>2656)
                     /locus_tag="VFK23_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7317509.1"
                     /translation="RYGTKLPAAKIRYATIGFCWGGSQSFQYAVTQPDLAAAVVYYGT
                     SPDLEALKNVKAPVIGLYGEDDARVNATVGPAETKMKELKKTYVTHTYKGAGHGFLRA
                     QDGRDGANLEASKQAWPATIEFLKKYAE"
BASE COUNT          584 a          779 c          807 g          486 t
ORIGIN      
        1 acatattcta atccatcata ccatgaaggt ggacttcctg ctttcgatga tttcggttgc
       61 aattcatgtc ccctcacggt acattgtgcg ggcacaaacg gcggctgaga acaggggtat
      121 tccatggcaa tcgtaagttt acagggcgtg aacgtcggct tcggcggtcc tctcctcctc
      181 gaaggggtct atctctccat cgacagggga gaacgggtct gcctcgtggg caggaacggc
      241 acgggcaagt ccacgatcct gcggctcatc accggcgagg tgaagcccga cgcgggaaag
      301 atcgtgttcc agcagagcgt gcgcgtcacg ctcctgaccc aggaggtccc tgaggcgctc
      361 tcgggaagcg tgttcgacgt cgtttccggc gctttcggag agcaggggaa actgctggcc
      421 gagtatcacc acttgagcgg aagcctcgtc catgatcaca gcgggaaatt gatggcaagg
      481 ctcgaggaag tccagcaccg gttcgaggct gcgggcggct ggcagatgca gcagaaagtc
      541 gaggagatcc tcacgcggct caggcttccg gaagacgcag agttttcgga gctctcgggc
      601 ggtctcaaac ggcgggtgct ccttgcaaag gcgctggcag gcgagccgga cctcctgctt
      661 ctggacgagc cgacgaacca tctggatatc gattcgattg catggctcga agagttcctg
      721 ctcgccttcg gggggacgct tcttttcgtg acccacgacc agatgctgat tcagaagctc
      781 gcaacgagga tcctcgacct cgaccgcggc agactcacga gctggcccgg caattaccag
      841 acctatctcg aacgcaagca ggcggcgctc gatgccgagg ccgtggagaa cgcgaagttc
      901 gacaagaagc tcgccgcgga agaggcctgg atcaggcagg gcgtcaaggc ccgaaggacg
      961 aggaacgaag gccgcgttcg cgccttgaag gagctgcgca ggatcaggag cgaacggcgc
     1021 gaggtcgctg gcgcggcgcg catgcagatg caggaggcgg agcgcacggg caagctcgtg
     1081 atcgaggcga ccgatgtcag atacgactac agcggcaggc cggtgatccg cgatttttcc
     1141 acgaccatca tccggggcga caagatcggc atcatcggac cgaacggctc gggcaagacc
     1201 acgctcttaa agctcctgct gggcgagctc aaaccgcaaa cagggaagat caaacacggc
     1261 acgaacctcg aggtcgctta cctcgaccag caccgggccc agctcgacga cgaaaagacc
     1321 gtgcaggaca acgtcgcgaa cgacagcgat tacgtaacca tcaacggcaa tcggcggcac
     1381 gtgatcggct atctcggcga ttttctcttc gcgcccgcgc gcgcccgcag cccggtgaag
     1441 gtcctctccg gcggagaaag gaaccggctc ctgctggcga agctcttcac gaaaccatcg
     1501 aacgtgctcg tcctcgacga gccgacgaat gacctggacc ttgagaccct tgatcttctc
     1561 gaagagctcc tgatggacta tcccggtacg gtgctcctcg taagccacga ccgcgcgttc
     1621 atcaacaacg tggtaacgag cacgctggtg ctcgagggcg aaggcatggt gggcgaatac
     1681 gtcggcgggt acgacgactg gatgctccag agcaggagga tgacgtcgga aaccgcggtc
     1741 cccgtcaagc cggaagcgaa gaaggagcct gcgcgccctc agaaggagag gccgcgcaag
     1801 cttaccttta aggagcagaa ggaacttgat gccctgccga agcgcatcga agcgctcgaa
     1861 gccgaacagc agcagatcca gaaggacctc gccgacccgg ccttctaccg cgagagcgga
     1921 aacaaggtat cggaatacaa gacgcggctt gaaacgctgg agaaggagct gactgaggca
     1981 tatacaaggt gggaagagct ggaggcgctg tcggtgaaat gaaatagaac catttccttt
     2041 ttacaggccg ccccaattta ttgccgtcat tgcgagcgaa gcaatccctt ttttacggtt
     2101 caggataaac tccgcgacct cgtagtccac gacaatttga aaaacgggat tgccacccct
     2161 gctttaatca tgcaggggca cgcttgcaag aaacgctcct cgcaatgaca cctaaagaag
     2221 tcgacttgcc gtttctctga gaccaaccgc cgttttcgcc atgtcactcc gcgtacttct
     2281 tcaaaaactc aatcgtcgcc ggccaggcct gtttcgatgc ttcgaggttt gccccgtcgc
     2341 ggccgtcctg ggcgcgaagg aacccatgtc ccgcgccctt gtaggtgtgg gttacatagg
     2401 tttttttcag ttccttcatc ttcgtttccg cgggtcccac cgtggcgttg acccgggcgt
     2461 cgtcctcgcc gtagagcccg atcaccgggg ctttgacatt cttcaaagct tccaggtccg
     2521 gagaagtgcc gtaatacacg actgcggcag caaggtcagg ctgcgtgacc gcatactgga
     2581 aactctgact tccgccccag cagaaaccga tcgtggcgta cctgattttc gccgcgggaa
     2641 gctttgtgcc gtagcg
//
LOCUS       DASSLD010000008         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_683861,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000008 DASSLD010000000
VERSION     DASSLD010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683861"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFK23_00095"
     CDS             <1..573
                     /locus_tag="VFK23_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET7317510.1"
                     /translation="VLQQKEFERVGDSTPLKADVRVIAATNKDLREKVKTGEFREDLY
                     YRLNVVAIALPPLRDRREDIPLLADVFIKKLNMKLGREITGISDDVLKIFMNHPWPGN
                     VRQLEHVLEHACILCRRNAITIDNLPADFMDSIQTAGLLARDGRADERGMILQALEKA
                     GGNKARTAKLLGISRRTIYRKMQELNITSP"
     gene            complement(1056..1421)
                     /locus_tag="VFK23_00100"
     CDS             complement(1056..1421)
                     /locus_tag="VFK23_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317511.1"
                     /translation="MKREDWFSRRLPFDPSRINPALLEVPAAIVDGLFRAHAVNADDK
                     AFIPGGIDEGAMFSCFQCSRFLYYSMLPFLAHTPLSRWKPAHLLTIRISRVVPEPQWP
                     FICSGFRVSELEDAAKTET"
     gene            1524..2192
                     /locus_tag="VFK23_00105"
     CDS             1524..2192
                     /locus_tag="VFK23_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7317512.1"
                     /translation="MKTYRITLADDHVLLRQGLKRIIEESGEMRIVGEAGDGLELISL
                     LNKTVPDMVVLDISMPNLRGIEVISEIKSRFPRVKTLIVTMHRDKEYLYQAFTSGADG
                     YLLKEDADLDLFAAIKKIRQGEAYVSPSLTQELVSYWRQAGKGEDKPSFTDECLTTRE
                     REVLKLVAEGKSSPQIAGLLSISIRTVDQHRANIKNKLRIRKTADLVKYAVSKGYIQV
                     LPHS"
     gene            2365..>2590
                     /locus_tag="VFK23_00110"
     CDS             2365..>2590
                     /locus_tag="VFK23_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317513.1"
                     /translation="MSVNNPAFQIPRRTVAGNENEFVKIFDPPQGSPATPELLDPTNP
                     VPEFMLANGSLAKVSTQHTVDSILYKNNPHT"
BASE COUNT          649 a          719 c          688 g          534 t
ORIGIN      
        1 gtgttgcagc agaaggaatt cgagcgtgtg ggagactcga ctcccctcaa ggcggatgtg
       61 cgggtcatcg cggcgaccaa taaggacctc cgtgaaaagg tcaagacagg cgagttcagg
      121 gaggacctct actaccggct caacgtggtt gccatcgccc tgcctccgct cagggacagg
      181 agggaagaca tcccgcttct ggcggacgtc ttcatcaaaa agttgaacat gaaactgggc
      241 agagagatca ccgggatatc ggacgacgtc ctgaagatat tcatgaacca tccctggccc
      301 ggcaacgtaa ggcaactgga gcatgtcctc gaacacgcct gcatcctttg ccgtcggaac
      361 gcgattacga tcgataatct gcccgcggat tttatggatt ccattcagac cgcgggtctt
      421 ctcgcacgtg acggacgggc cgatgaacga gggatgattc ttcaggccct ggaaaaggcc
      481 ggagggaaca aagccaggac cgccaaactc ctcggcataa gccggcggac catttaccgg
      541 aagatgcaag aactcaatat cacgagccct tgatagcgat aatttttcgg atgaggattc
      601 ttaacttaac ctgaggccgg aggaagagac gggcttcgcg tcttcgtccg cagacggttt
      661 cgacgtaact tcaaatccca ataaatacgg gccatagtca cggcgtgcgg attttgaacg
      721 ccgacctttc cgcgccgtcg gcacgcgggg tcatctacac gagtaccggc tcgcatatcc
      781 ataccatcac ccttttcaca gcagcagctc atcgatatcg ccaacggttc ggccataatg
      841 gtggtttctt ccgcctcagg cgttcccatt catacgcata cgtggaccat tcaaaagccg
      901 tgatctcctt ccacgcagca gcctctcaaa gataaagccc ttctcccgca tcctcttaaa
      961 aatctgtgcc atggtacgac tgtcctgaca cacatccgtg ccatggcaca ctctttttct
     1021 tcttcaacga aatctctttt tccttcccgt attcctcatg tctctgtttt tgcggcatct
     1081 tcaagctcgc tcaccctgaa accggaacag atgaacggcc attgcggttc tggcacgact
     1141 cttgatatac ggatcgtcag tagatgagcc ggtttccagc gggaaagggg agtatgagcc
     1201 aaaaagggga gcatagagta atacaaaaac cggctgcatt gaaagcagga aaacattgcc
     1261 ccttcgtcga ttccccctgg aataaatgct ttgtcgtcgg cattgacagc gtgagcgcgg
     1321 aaaaggccat ctactattgc ggccggaact tcgaggagtg cgggatttat tcgagaagga
     1381 tcaaagggga gccgtcgtga aaaccagtct tcgcgtttca tagtgcgtcc tccttttgtt
     1441 caacaaacgc cggtagaact caacgcaacg agctctcgag ttttaaaacc catggggacg
     1501 aggcctggag gacatggacc gcgatgaaaa cttatcgcat cacattggcc gacgatcacg
     1561 tcctgcttcg ccagggtttg aagcggatca ttgaggaatc cggggagatg cggatcgtcg
     1621 gcgaggcggg agacgggctc gagctcatct ctctcctgaa caagacggtt cccgatatgg
     1681 tcgtactcga catctccatg ccgaacctca gggggatcga ggtcatcagc gagatcaaga
     1741 gccggtttcc ccgggtaaag actctgattg tgacgatgca ccgggacaag gagtatctct
     1801 atcaggcgtt cacctccggc gccgacggct accttctgaa agaggatgca gacttggatc
     1861 tcttcgcagc cataaaaaaa atccggcaag gggaggccta tgtgtcgcct tccctcacgc
     1921 aggagctggt atcatactgg cgccaggcgg gcaagggaga ggacaagccc tcttttacgg
     1981 atgaatgcct gacgactcgc gagcgggagg tgttgaagct ggtcgcggaa gggaaatcat
     2041 caccgcagat cgccgggctt ctctccatca gcatacgcac cgtagatcag caccgcgcca
     2101 acattaaaaa caagctgagg atcaggaaaa cagccgacct cgttaaatac gccgtcagca
     2161 agggatacat tcaggttctt cctcactcct gatgcgctcc ggaatccgga aatgtcatgg
     2221 ttgcaggccc gtcaatcagc gggcatggta tacaagtaat ctcgtccgta cggcgcttga
     2281 caagccgcag actcgcccgt acttgaaaga acataaaaac ccttgtgagc atccagggac
     2341 agacaatgaa ggaggacaaa agtaatgtca gtcaataacc ctgcattcca aatcccccgg
     2401 agaacggtgg cgggcaatga aaacgaattc gtcaagattt tcgacccgcc gcaggggtcg
     2461 cctgccacgc ccgaacttct ggacccgacg aacccggtcc cggaattcat gctggcgaac
     2521 ggctcgctgg cgaaggtgtc aacccaacat accgtggata gtatcctgta caagaacaac
     2581 ccccataccg
//
LOCUS       DASSLD010000009         4656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_617915,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000009 DASSLD010000000
VERSION     DASSLD010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617915"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VFK23_00115"
     CDS             complement(<1..715)
                     /locus_tag="VFK23_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7317514.1"
                     /translation="MIPIFIASNTGYSGRTFVSLGLAMKLIEQGFKVGYLKPLGRVPV
                     KKGKEVYDADALFIRETLGLEEPLEVISPFVQTYESQTLLFGGGLRDVRKDILDAFKT
                     LKKKDFVIIGGSSDLFDGSLLGVNALSLIRDMKAYLLMVEPWRGEGSADVLYGSSRFL
                     GERFAGGVINKVPANAAQHVRETVRPFLEKKGVRVFGVFPKDHALEAVTVRQLNDMLG
                     GKVLCCEGGLDEYVENFSVG"
     gene            complement(850..2949)
                     /locus_tag="VFK23_00120"
     CDS             complement(850..2949)
                     /locus_tag="VFK23_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET7317515.1"
                     /translation="MLETLFHPASIAVVGASNDQRKVGHSVLRNLCQFRFPGRLYPVN
                     PAGGEILGHRAYAKIGDIGQPVDLAVIVIPAPFVPAALRECAAAGIRSAIVISAGFKE
                     AGKEGTLLEEELKTIVREQGIRVLGPNCLGLINTANDMNATFAADMLPRGTIGFFSQS
                     GAMGIAIMDWAIGNDVGFSKFVSLGNKADLSEIDIIEYFMDDPETTLILGYIEDVVDG
                     GRFMDIAARATKVKPVILLKSGGTEAGARAASSHTGALAGSDTAFDAAFRQTGVVRAQ
                     GVQDLFDTALAFAEGKLPAGKGLLIVTNAGGPGIIAADAAERLGLELPHMTRESIIAM
                     AKKLPPNATVFNPVDVIGDATSERYAAVLEQAARDPNVDGILVILTPQAMTDTEKTAD
                     AVIAAARSTDKPVIASFMGEKRVRPAIDKLKDASVPNFSYPELAVKAFRRLTDQWSWK
                     HERPEELVSCWYNFDAAAETIAEVLKNGIYRVGEEEAMRILTYYGFQFPRRALARTSR
                     EAAQQASRIGFPVVMKVSSPDILHKTDVGGVRVNVRTPREAEEAFSDITANSRRLMRD
                     AFIKGVMVYEMAKGGKEVILGVTYDRTFGHMIMFGLGGIYVEVLKDVSFRIVPVSRRD
                     AAAMVSEIRTAGLLRGARGERPADLDAIATNIVNLSCLIRDFPEIQELDINPLMVMER
                     GAVALDARIIFKQPEKK"
     gene            complement(3060..3860)
                     /locus_tag="VFK23_00125"
     CDS             complement(3060..3860)
                     /locus_tag="VFK23_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317516.1"
                     /translation="MMNQSDFDFDVVDPDTAERSIPVYPVAQWHNGQQALKALGGVQH
                     AGGVVLPKKYVSEGSRLPGWTEERLTFRSGKEETALTAQKLIMSPIRTRFRWFARQGT
                     DTVYYPRSAYVMGAHLRGHLQVLAAVKGANAPIVVTFKGKASQTFEALLKDFSAKVVQ
                     TANRTAPKGKALPRFAFWMSVSPGPHTKAGSPGRDSFITPPTLDLPAEISRDYLRSLY
                     IGRDNLIRFQEWYREAEPWIAAWERTGVEQTDTAPESEEEIAGGETQP"
     gene            4036..>4656
                     /locus_tag="VFK23_00130"
     CDS             4036..>4656
                     /locus_tag="VFK23_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010872336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HET7317517.1"
                     /translation="MTIPNWQERIAEIKALYPEKFPTEERIFSAIHPGNRVFIGTGCG
                     EPQYLVRALIDYVRSHPKAFYDAELLQVWTLGVAPYADQKFKDNFRYNSFFIGNNTRD
                     AVNRGLADYTPVFLSQVPGLFQKKLVPVDVALIQTSLPDEHGFMSLGISVDIVKAAAE
                     NAPLVIAQVNPRMPRVLGETFINIKDVGFVIPCDEPLLEFEATVADE"
BASE COUNT          852 a         1471 c         1411 g          922 t
ORIGIN      
        1 cgcccacgga gaaattttcg acgtactcgt cgagaccgcc ctcgcaacag agcacctttc
       61 cgcccagcat atcgttaagc tgccggacgg tcaccgcctc gagagcatgg tccttgggaa
      121 agacgccgaa gaccctgacg cccttcttct ccaggaaggg ccttaccgtt tcccgcacgt
      181 gttgggccgc gttcgccggc accttgttga tgacgccgcc cgcgaaccgc tcgcccagga
      241 accgggagga gccgtagagc acgtccgcgg agccctcgcc gcgccagggc tcgaccatga
      301 ggaggtacgc cttcatgtcg cgtatgagcg agagggcatt cacgcccaaa agcgagccgt
      361 cgaacaggtc gctcgatcct ccgatgatca cgaagtcctt cttcttgagg gtcttgaatg
      421 catcgaggat gtcttttctc acatcccgga gaccgccccc gaagagcagg gtctggctct
      481 cataggtctg gacgaagggc gagatcacct ccaggggctc ctcgagcccg agcgtctccc
      541 tgatgaacag ggcgtcggcg tcgtagactt ccttgccctt ctttaccggc accctgccga
      601 ggggcttcag atacccgacc ttgaaaccct gctcgatcag cttcatggcg agaccgaggg
      661 agacgaaggt ccggcccgag taaccggtat tggatgcgat gaatatgggg atcatgagaa
      721 cctccttatc acaaattcgg aatgccgaat gcggaatgca gaattaacgt cgacggtaat
      781 tacatcggct cctctctccg aggttgggtg atggtggtta cattccgcat tccgaattcc
      841 tcattatatt catttcttct caggctgttt gaatatgatt cttgcatcga gggctaccgc
      901 gccccgctcc atcaccatga gcggattgat gtccagttcc tggatctcgg ggaagtcgcg
      961 gatcaggcat gagaggttca cgatgttggt ggcgatggcg tcgaggtccg cgggccgctc
     1021 cccgcgcgcg ccgcgcaaaa gcccggcggt ccgtatctcg ctcaccatgg cggcggcgtc
     1081 ccgacggctg acggggacga tccggaacga cacgtccttc aggacctcga catagatgcc
     1141 gcccaggccg aacatgatca tgtggccgaa ggtccggtca taggtgacgc cgaggatcac
     1201 ttccttgcca cccttcgcca tctcgtagac catcacgccc ttgatgaagg cgtcgcgcat
     1261 gagacgccgg gaattcgctg tgatgtcgct gaaggcctcc tcggcctccc ggggcgtcct
     1321 gacgttcacg cgcacgcccc ccacgtcggt cttgtgcagg atgtcgggcg acgacacctt
     1381 catcacgacg gggaaaccga tccgcgacgc ctgctgcgcg gcctcgcgcg aggtccgggc
     1441 aagagcgcgc ctcgggaact gaaatccgta ataggtcagg atccgcatgg cctcttcctc
     1501 gcccacccgg tagatgccat tcttgaggac ctccgcgatc gtctccgcgg cggcatcgaa
     1561 gttataccag cagctcacca gctcctcggg ccgctcatgc ttccaggacc actggtccgt
     1621 aagccgcctg aaggccttga ccgcgagttc ggggtaggag aagttcggga ccgacgcatc
     1681 cttcagcttg tcgatggccg ggcggacccg cttctcgccc atgaaggacg cgatgaccgg
     1741 tttgtccgtg gaccgtgcag cggcaatgac cgcatcagcc gtcttctccg tgtccgtcat
     1801 ggcctggggc gtgagaatga cgaggatacc gtccacgttc gggtcccgtg cggcctgctc
     1861 gagcaccgcc gcgtaccgct cggacgtagc gtcgccgatc acgtccaccg ggttgaagac
     1921 cgtggcgttc ggcggaagct ttttggccat ggcaatgatg ctttccctgg tcatgtgcgg
     1981 gagttccagc ccgagccgct ccgcggcgtc ggccgcgatg atccccggcc ctcccgcgtt
     2041 cgtcacgatg aggagcccct ttcccgcggg cagtttcccc tcggcaaagg ccagcgccgt
     2101 gtcgaaaagg tcctgcacgc cttgggcgcg cacgacgccc gtctgccgga aggccgcgtc
     2161 gaaggcggtg tccgatccgg caagggcccc ggtgtgcgac gatgccgctc gggcccccgc
     2221 ctcggtgccg ccggacttca ggaggatgac aggcttgacc ttcgtcgccc tggcggcgat
     2281 gtccatgaac cgcccgccgt ccacgacgtc ctcgatgtag cccaggatca gggtcgtttc
     2341 gggatcgtcc atgaaatatt cgatgatgtc gatttccgag aggtcggcct tgttcccgag
     2401 gctcacgaac ttcgagaacc ccacatcgtt gccgatggcc cagtccatga tggcgatgcc
     2461 catggcgccg gactgggaaa agaagccgat tgtcccccgg ggcagcatgt cggcggcgaa
     2521 ggtggcgttc atgtcgttcg cggtattgat gaggccgagg cagttgggcc ccaggacccg
     2581 gatgccctgc tcccggacga tcgtcttcag ctcttcttcg agcagtgtgc cttccttgcc
     2641 cgcctccttg aagcccgcgc tgatcacaat ggccgagcgg atgccggctg ctgcgcactc
     2701 ccggagcgcg gcgggaacga atggcgccgg gatgacgatg accgcgaggt cgacgggctg
     2761 gccgatgtcc ccgatcttcg cataggccct gtgcccgagg atctcgccgc cggcagggtt
     2821 tacggggtaa agccgccccg gaaacctgaa ttggcagagg tttcggagga ccgagtgccc
     2881 cacttttctc tgatcgttcg acgcgccgac cacggcgatg gaggcgggat ggaagagggt
     2941 ctcgagcata atgccttaag tgtagtagag aaacggggaa gggtcaaggg aaggaaaaat
     3001 cagccctcct tcctgcaaag gagaaagggc tgaggactga atttcgccgc gtatcaggtt
     3061 cagggttggg tttcgccgcc cgcgatttcc tcttcgctct cgggagccgt gtcggtctgc
     3121 tccacacccg tcctctccca ggcggcaatc cagggctcag cttcacggta ccactcctga
     3181 aaccggatca ggttgtcgcg accgatgtac agggagcgaa gataatcgcg ggatatttcc
     3241 gcaggcaaat cgagggtcgg aggcgtgata aatgaatccc ggcccgggct tcccgctttt
     3301 gtatgaggcc ccgggctgac gctcatccag aaggcgaagc gcggcagcgc cttgcctttg
     3361 ggcgccgtcc ggtttgccgt ctggacgacc tttgccgaaa agtccttcaa gagtgcctcg
     3421 aaggtctgcg acgccttgcc tttgaaggtg acaacaatgg gcgcgttcgc gcccttgacc
     3481 gcggcaagca cctgcaggtg cccgcgcaga tgtgccccca tcacataggc gctgcgggga
     3541 taatagacgg tatcggtccc ttgccgtgcg aaccaccgga agcgggtgcg gatcggggac
     3601 atgatgagct tttgcgccgt caacgccgtc tcttccttgc cgcttctgaa ggtcagccgt
     3661 tcttccgtcc agcccggaag cctcgatccc tcagaaacat attttttcgg cagtactacg
     3721 ccgcccgcat gctgcacgcc gccaagggcc ttcagggcct gctggccgtt gtgccactgt
     3781 gcaaccggat acaccggaat ggagcgttcg gcggtatcgg ggtccacgac atcgaagtcg
     3841 aaatcagact gattcatcat gtatcctcct gtagcgttaa ttggcaattc gccgaaacgg
     3901 tatcataccg tgtcctctgc cgcaagctac ccgtttgggt agggtcaaac ctgtttatac
     3961 ttcgccgggt ttgcaggttt ttgcggcctg cacggttctg ctatacttct taatagctcc
     4021 ggaggcacag gtgtcatgac cataccgaac tggcaggaaa ggatcgcaga gatcaaggcc
     4081 ctctatcccg agaaatttcc gacggaagag cgcattttca gcgccattca tcccggaaac
     4141 cgggtcttca tcgggacggg ctgcggggag ccgcaatacc tggtccgggc gctcatcgac
     4201 tacgtccggt cccaccccaa ggccttctac gacgccgaac tgctccaggt ctggaccctc
     4261 ggggtggcgc cctacgccga ccagaagttc aaggacaatt tccggtacaa ttccttcttc
     4321 atcggcaaca atacccgcga cgcggtgaac cgggggcttg cggattatac gccggtcttt
     4381 ctgtcccagg tccccggtct gttccagaaa aaactcgtgc ccgtggacgt ggccctgatc
     4441 cagacgtcgc tccccgacga gcacgggttt atgagcctcg gcatcagcgt ggacatcgtg
     4501 aaggcggcgg cggagaacgc acccctcgtc atcgcccagg tgaacccccg gatgccccgc
     4561 gtcctcggtg aaacgttcat caacatcaag gacgtgggtt ttgtcatccc ctgcgacgag
     4621 ccgctcctgg aattcgaggc caccgtcgcc gacgag
//
LOCUS       DASSLD010000010         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_813112,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000010 DASSLD010000000
VERSION     DASSLD010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813112"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..574)
                     /gene="mreD"
                     /locus_tag="VFK23_00135"
     CDS             complement(62..574)
                     /gene="mreD"
                     /locus_tag="VFK23_00135"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET7317518.1"
                     /translation="MKPRIYFIIFLLIIPIQASLFSRLSLAGIKPDLALAVIYIIGLL
                     TSPGEAALAGIGIGLVQDIGSASFIGLNGFTRGLVGLFAGLFGRRVLDISSPSNFIFL
                     TAFSLAEGFFIAFFMQVIYGSVPIFSMFFTSMLPQALYTGVLGILLLRFIGNRNVLST
                     LRRHALQKEL"
     gene            complement(571..1377)
                     /gene="mreC"
                     /locus_tag="VFK23_00140"
     CDS             complement(571..1377)
                     /gene="mreC"
                     /locus_tag="VFK23_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET7317519.1"
                     /translation="MPEFIVKHKKFLILFILFMILFWLITFQVKAGRLTFMEKPVLAV
                     SGFFERVITSPFRFAESAVTGYIFLIHTERENGRLKEEAGQLKLEKSITRELIIENER
                     LRELLGFRKLHPPASVMAQVIAKESSPSSRTVTINKGADDGIQKDMAVIAPMGIVGRV
                     QAVLSGTAKVILLTDPGSTLAVRVQRNREEGLLEGKIITCALKYVSYYADIQEGDLLV
                     TSGLDGIYPRGLPVATVTKVDRYEASAFQTVVAEPAVRFSRLEEVLVLRK"
     gene            complement(1395..2420)
                     /locus_tag="VFK23_00145"
     CDS             complement(1395..2420)
                     /locus_tag="VFK23_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET7317520.1"
                     /translation="MFINTIMGWFSNDMAIDLGTANTLVYVKGKGIVCNEPSVVAISA
                     KTGRVLSVGAEAKRMLGRTPGDVKTIRPIKDGVISDFDITGEMLKYFIQRVHNRKSFM
                     RPRIVIGVPSGITQVEQRAVKDAAITSGGRDVYLIEEPVAAAIGTGLPISEPSGNMIV
                     DIGGGTTDIAVISMDGVVYSKAIRVGGDKMDEAIINYIKRKYNLLIGEMMAESIKLEI
                     GSAYKTDSSRQTMEIKGRDLVSGIPKTLILDDSEVRESLAEPVSQILNGIKLALENTP
                     PELSADIVDKGIVLAGGGALLKGLDVYLREETSLPIIIAEDPLTCVALGAGKVLDDEE
                     ILKKVIM"
BASE COUNT          592 a          715 c          706 g          616 t
ORIGIN      
        1 tgaacgccgc cagaaacggg agccttctct ggatgtcgat gggactgtcc tgctggtaca
       61 tctacaactc cttctgaagg gcgtgccgtc tcagcgtgct caggacgttc ctgttgccaa
      121 tgaaccgcaa cagtaggatg ccgaggaccc cagtatagag cgcttgcggg agcatgctgg
      181 tgaagaacat actgaagatc ggaacggatc cgtaaatcac ctgcatgaag aacgcgatga
      241 aaaatccttc cgccagggag aaagccgtca ggaagataaa attcgatgga ctcgatatat
      301 cgagcaccct cctgccgaag aggcctgcga acaaacctac cagtccccgc gtgaatccgt
      361 tcaggccgat gaaactcgcc gatccgatat cctggactag gccgatgccg atgcccgcga
      421 gcgcagcctc tcccgggctg gtgagcagtc caatgatata aatcacggcc agggcaaggt
      481 caggcttaat gccagccaaa gaaagacggc tgaacaggct cgcctgaata gggatgataa
      541 gcagaaagat gatgaaataa atgcggggtt tcatttccgg agcaccagta cttcttccaa
      601 acgcgagaat ctgacggcag gctccgccac aacggtctga aacgcgctgg cctcgtatct
      661 gtcgactttg gtcaccgtgg cgacggggag tcccctgggg tagatgccat cgagtccaga
      721 ggtaacgagc aggtcgcctt cctggatgtc ggcatagtaa gaaacatact tcagagcgca
      781 ggtgataatc ttgccctcga gaagtccctc ttccctgttc ctctgaaccc tgacggcgag
      841 cgtgctcccg gggtccgtga ggagtattac ctttgccgta cccgagagca cagcctggac
      901 ccttccgacg atgcccatcg gggcgattac cgccatgtcc ttttggatgc catcgtctgc
      961 tcctttattg attgttaccg tgcgcgaaga gggtgaagat tccttggcga tcacctgtgc
     1021 cataaccgat gccgggggat gaagcttcct gaagcctaac agctcgcgca agcgttcgtt
     1081 ctcgatgatg agttctcttg tgatggactt ctcgagtttc agctgtcctg cctcttcttt
     1141 caaacgtcca ttttcccgct ccgtgtgaat gaggaaaata tatcccgtga cagcagattc
     1201 cgcgaagcga aagggactgg taatgacgcg ttcaaaaaaa ccggaaaccg cgaggaccgg
     1261 cttctccatg aacgtcaacc gtccggcctt cacctggaac gtgatgagcc agaacaggat
     1321 catgaagagg atgaacaaga taagaaattt tttatgcttt acaataaact caggcattcg
     1381 gacagacgct tgttctacat gattactttt ttcaggattt cctcatcatc gagcaccttg
     1441 cctgcgccga gcgctacgca agtaagggga tcttctgcga taatgatggg gagcgatgtt
     1501 tcctctcgga ggtagacgtc caggcccttt agcagcgctc cgccacctgc gagcacaatt
     1561 cccttgtcca caatgtcagc tgagagttcc gggggtgtgt tctcgagcgc gagctttatc
     1621 ccgttcaaaa tctgactaac aggctcagca agcgactcgc ggacttcact gtcgtcgagg
     1681 atcagcgtct tggggatgcc ggaaaccagg tcgcggcctt tgatctccat ggtctgccgg
     1741 gagctgtccg ttttatacgc agatccgatc tcgagcttga ttgactcggc catcatctcg
     1801 ccgataagca ggttatattt gcgcttgatg taattaatga tagcctcgtc cattttgtct
     1861 ccccccaccc ggatggcctt gctgtagact acgccgtcca tggagatgac ggcgatatct
     1921 gtagtgccgc cgccgatgtc cacgatcatg ttgcccgaag gctctgatat gggaagaccg
     1981 gtcccgatgg ctgcggcgac gggttcttcg atgaggtaga catcacgccc tcccgatgtg
     2041 atggccgcat ccttgaccgc gcgttgttcg acctgcgtga tccctgatgg aacgccgatc
     2101 acgatgcgag gccgcatgaa ggatttccgg ttgtgtaccc gctggatgaa gtatttaagc
     2161 atctcgccgg ttatgtcgaa gtccgaaatc acaccgtcct tgatcggacg aatcgtctta
     2221 acatcgcctg gcgtacggcc gagcatccgt ttggcttcgg cacctacgga aagcactctg
     2281 ccggtcttcg ctgagatggc aacgacagaa ggctcgttgc acacgatgcc cttgcccttg
     2341 acgtacacca gcgtattggc cgtcccgagg tcgatggcca tgtcgttcga aaaccatccc
     2401 ataatagtgt ttatgaacat cgttagaaaa ggctcctttc gaatgcggat cattgagtga
     2461 tgcacaggga ccggcggacc gatcctatca aatccgggct attgtcctga gactccctga
     2521 atttaatctc tcttgacgac tgatcgtgct gccgagcgtg ctatatatca cgtgttatcg
     2581 tgtcctgttc ctctttttca atcgaaggag gaactgtttg tatatcggg
//
LOCUS       DASSLD010000011         4948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1009667, whole genome shotgun sequence.
ACCESSION   DASSLD010000011 DASSLD010000000
VERSION     DASSLD010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009667"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VFK23_00150"
     CDS             complement(<1..808)
                     /locus_tag="VFK23_00150"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HET7317521.1"
                     /translation="MTPQLQQAIKLLQLSRLELVQSVSQELVENPVLEELPPEAEGSE
                     ATPEGEDTTYSEVTPQETPAEAPPEREGEQELKSDLELGPQWDEYLNEMGDGRDFGYA
                     EADEKELPSYDQTLTRLPSLADHLLWQLHLTSSDPDVVRGGEWIIGNLDDDGYLRATL
                     DELAGQSGIGLPAMTRAMELIQGFDPAGVAARDLRECLLIQVRQLGLNDTLVERIVVG
                     HLPDLEKRKYANIAKALNVTPQEVMEASQIIINELEPKPGRPYLASDTNYV"
     gene            complement(949..1671)
                     /gene="lptB"
                     /locus_tag="VFK23_00155"
     CDS             complement(949..1671)
                     /gene="lptB"
                     /locus_tag="VFK23_00155"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HET7317522.1"
                     /translation="MQALEANNLEKSYRGRKIVRGVSLQVMNGEVVGLLGPNGAGKTT
                     TFYMVVGLVRPDLGSVLLGGMDITKMPMYRRAKNGIGYLPQEPSVFRKLTVGENLMAV
                     LETMNLSSIERKERRDTLLEELGIAHLVDSKAYTLSGGERRRVEIARALTLSPKFILL
                     DEPFAGIDPLAVQDIQGIISFLKNKGIGILITDHNVRETLAITDRAYIINEGEIIEAG
                     TSADITANPRVRQFYLGEAFRL"
     gene            complement(1686..2180)
                     /gene="lptA"
                     /locus_tag="VFK23_00160"
     CDS             complement(1686..2180)
                     /gene="lptA"
                     /locus_tag="VFK23_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HET7317523.1"
                     /translation="MSRRYFRNSLLVIASVAVLAAVPALAAPLVTKEAEPIVITSNRM
                     EAEKLGEKVTFSGNVELKKEGMTVTSDQMVVFYDPGSKNIREIVARGNVVVRKEGRIA
                     LANLATYSLREEKIVLTGDARIIENENQVGGQKIILFMRDDRSIVEGGKVLLYQDKLD
                     KSRK"
     gene            complement(2155..2715)
                     /gene="lptC"
                     /locus_tag="VFK23_00165"
     CDS             complement(2155..2715)
                     /gene="lptC"
                     /locus_tag="VFK23_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HET7317524.1"
                     /translation="MIRTVSKVLAVLLVAGACVYGGYLLIRGKEVALPALFPPAPGPR
                     LVISMETFTLTQTEDGRVAWVLNAHTADLYENKEAKLKDVRIVFKDSDSREATMTGDL
                     ASMDTGSGNASVRRDTQDVRIVTSDGYLLTTDSLFWRAGERQVWTADPFKLLGKEIYL
                     EGVGITADVDIRKIAVKDNVKAVLQK"
     gene            complement(2836..4092)
                     /locus_tag="VFK23_00170"
     CDS             complement(2836..4092)
                     /locus_tag="VFK23_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7317525.1"
                     /translation="MMQQNSFEQRKGMLIFLISLVLGAAVAVGAFAGHLAAEGEGSGV
                     LLALVLVPLVALVAYLLYRKMIFPSYRRLEDANLELHLKQEELLDIKDDLFIKFLGVY
                     DINFAANSPRLFADRLKDVADITARVMGADACFVFLYDRKKDDLLLSATNGVQEAAIG
                     NARVPLGEGIEGWVARHLEPLMLKDLRADARYRPIPGLSLDGYQSVYCLPLYVYSNGA
                     LVGAMEVLFRKAKEFTDEEINFFTTLTGILSTTIQNEQMQAELRKMNLELEQWVTEKT
                     EELRASEERYRTLVENACESIFLLTGNGDIVFANDQASRLAGHSKYDLVHKNFFELFV
                     DPAEPREILADVAQGRQSLRQGDLRLAGRGAVPVSVSAVGLSLLGKQFIQAVVRDVST
                     HLRLEQLLAEKDRELAALRARVEDTR"
     gene            complement(4119..>4948)
                     /locus_tag="VFK23_00175"
     CDS             complement(4119..>4948)
                     /locus_tag="VFK23_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7317526.1"
                     /translation="TIISVSCLKHTYPDTTEIHICGLDFVVNRGERIAILGGNGSGKT
                     TLLYHILGLLKPDEGRVSVFGVNPAQRYNEIRKRIGVLLQNVDDQILSPTVWDDVSFS
                     PRNYGYGREQIERMVEQVLEELAISHLRDKICHYLSGGEKRKVALAGALVMRPELLIL
                     DEPFEGLDNRSRAELVTLLNSYNRAGMTIIMSTHDLNLVAAFADRVYVLARGRGVITT
                     GTPNEIFMQVDALRATNIDPPILTELFLKLRERGVAVVDVPIHLDQAVNDLVRLAGD"
BASE COUNT          961 a         1542 c         1372 g         1073 t
ORIGIN      
        1 ccacataatt cgtatcggag gccaggtacg gcctgcccgg cttgggttcc agctcgttga
       61 tgatgatctg cgacgcctcc atgacctcct ggggggtaac gttcagggcc ttggcgatgt
      121 tcgcatattt tctcttttcc aggtccggaa ggtggccgac gacgatccgt tcgaccagcg
      181 tgtcgttgag accgagctgt cgtacctgga tgagcaggca ctcgcgaagg tcgcgcgcag
      241 cgacccccgc ggggtcgaag ccctggatga gttccatcgc ccgcgtcatg gccggaaggc
      301 cgatgccgga ttgccctgcg agttcatcga gcgtggcgcg gagatagccg tcatcgtcca
      361 ggttgccgat gatccactcc cctcccctca ccacatcggg gtccgacgac gtcaggtgga
      421 gctgccacag gaggtggtcg gcgagcgacg ggagccgcgt cagggtttgg tcgtaggacg
      481 gaagctcttt ttcgtcggct tccgcgtaac cgaagtcccg tccatcgccc atctcgttca
      541 aatactcgtc ccactggggc ccgagttcga gatcgctctt gagctcctgc tcaccctccc
      601 gttcgggcgg agcctcggca ggcgtctcct ggggggtcac ttcggaatag gtcgtgtctt
      661 cgccctcggg ggtcgcctcg gaaccttcgg cctcgggagg cagttcttcg agaaccgggt
      721 tttcaacaag ctcctgggaa accgactgga ccagctcgag cctcgacaac tgcagcagct
      781 tgatcgcctg ctgcagctgc ggcgtcataa tgagcttctg ggaaagtttt aattcaagcc
      841 gtgcttccat gatttaaaat gcaggagcca gaatgagtgc tgactgttaa ctccttccct
      901 tcttctggat tctgaatcca gactctgact tcgcatctct ttctttaatt ataaccgaaa
      961 cgcctcaccc agataaaatt gccggacccg cgggttggcg gtgatatccg ccgatgtccc
     1021 cgcctcaatg atctcccctt cgttgatgat ataggcccga tcggtgatgg caagggtctc
     1081 cctcacatta tggtcggtga tcaggatgcc gatccccttg ttcttgagaa acgatatgat
     1141 tccctgaatg tcctgcaccg caagggggtc gatcccggca aagggctcat cgagaaggat
     1201 gaatttcggg gacagggtga gcgcccgcgc aatctcgacc cgccggcgct ccccccccga
     1261 cagggtatag gccttgctgt caacgagatg cgcgatgccc aattcctcca gcagcgtgtc
     1321 ccgtcgctcc tttcgctcga tcgaggaaag attcatggtc tccagaacgg ccatcaggtt
     1381 ctccccgacg gtgagctttc gaaaaaccga cggctcctgg ggcagataac cgatgccgtt
     1441 tttcgcccgt cgatacatgg gcatcttcgt gatatccatg cctccgagga ggacgcttcc
     1501 cagatccggc ctcacgagcc cgaccaccat gtagaatgtc gtggttttgc cggccccgtt
     1561 cggaccgagc aggccgacga cttctccatt catgacctgt aaactgactc cgcgaacgat
     1621 ctttctgccg cggtagctct tctccagatt attcgcctca agcgcctgca tgcatccctt
     1681 ttcgattact tccgcgattt gtcgagcttg tcctgataca acaggacctt tccaccctct
     1741 acgatgctgc ggtcgtcgcg catgaacagg attattttct ggccgccgac ctggttttca
     1801 ttttcgatga tgcgcgcatc cccggtcagg acgatttttt cttcgcgcag ggagtatgtg
     1861 gccaggttcg ccagcgcgat cctgccttcc ttgcggacca cgacgtttcc ccgtgccacg
     1921 atctcccgga tgttcttgga accgggatcg taaaatacga ccatctgatc cgaggtcacc
     1981 gtcattccct cttttttgag ctccacattc ccgctgaagg tcactttctc gcccagtttt
     2041 tccgcctcca tgcggttgga ggttatcacg atgggctccg cctcctttgt caccaatggc
     2101 gcggcgaggg cagggaccgc cgccaggact gcgacggacg cgataacgag caggctattt
     2161 ctgaagtacc gccttgacat tatctttcac cgcgatcttg cgaatgtcga catcagccgt
     2221 gatgccgacc ccctcgagat agatctcttt gcccagcagc ttgaacgggt ccgccgtcca
     2281 gacctggcgt tcaccggccc tccagaataa tgaatcggtg gtcaaaaggt aaccgtcgct
     2341 cgttacgatc ctgacgtcct gcgtatcccg gcgcacggaa gcgtttccgc tccccgtgtc
     2401 catggacgcg agatcgccgg tcatggtcgc ctcccgcgaa tcggaatcct tgaaaacgat
     2461 ccgtacgtcc ttcagtttcg cttccttgtt ttcataaaga tcggcggtgt gggcgttcag
     2521 gacccacgcg acccttccgt cttcagtctg cgtcagcgtg aacgtttcca tgcttataac
     2581 gagacggggg cccggggccg gaggaaacag ggcagggagt gccacttcct tgccccggat
     2641 gagcagataa ccgccataga cgcatgcacc cgcgacaagg aggaccgcga ggacctttga
     2701 aacagttctg atcaccgtca cactcacaag agcatatggc ggcttatcca gccgccatat
     2761 gcataattta taccacaggg cctggctact gtaaagataa aaatcaaacg gcttcgtcga
     2821 gggtggaaac ggacctcacc tcgtatcctc cacccgggcc ctcagtgcgg ccagttcccg
     2881 gtccttttcc gccagaagct gttcaaggcg caaatgcgtc gatacgtctc gcacgaccgc
     2941 ctggatgaac tgctttccca ggagcgacag tcccacggcg ctcaccgaaa ccggcacggc
     3001 gccccgcccg gccagcctca aatcgccctg cctcagcgac tggcggccct gcgcgacgtc
     3061 cgcgagaatt tcccgcggtt cggcgggatc gacgaaaagc tcgaaaaaat tcttatgcac
     3121 gaggtcatac ttggaatggc cggcgagccg cgatgcctga tcgttcgcaa agacaatgtc
     3181 accgttcccg gtaaggagga agatcgattc gcaggcgttc tccaccagcg tacgatatcg
     3241 ctcttccgac gcccgtagtt cctcggtttt ttcggtcacc cactgctcca gctccagatt
     3301 catcttccgc aactccgcct gcatctgttc gttctggatc gtggtcgaca gaatacccgt
     3361 caacgtcgta aagaagttga tctcctcatc cgtgaactcc ttggccttcc ggaacaacac
     3421 ctccatggcc ccgaccaagg ccccgttcga atacacgtag aggggaagac aatagaccga
     3481 ctggtatccg tccagcgaca ggccgggaat tggccggtac cgcgcatcgg cgcgcagatc
     3541 cttcagcatg agcggttcaa gatgccgcgc cacccatcct tcgatcccct cgccgagggg
     3601 aacccgcgca ttaccgattg ccgcttcctg aacgccgttg gtcgccgaaa ggaggaggtc
     3661 gtccttcttc cggtcataga gaaagacaaa gcatgcgtcg gcccccatga ccctggccgt
     3721 gatgtcggca acgtccttca gacgatccgc gaagagcctt ggagaattgg ccgcaaagtt
     3781 gatgtcgtac acgccgagaa acttgatgaa caggtcgtcc ttgatgtcca gaagctcctc
     3841 ctgcttcaaa tgcagttcga ggttggcgtc ctccagacgg cgataggagg ggaaaatcat
     3901 cttccggtac agcaggtatg cgacgagcgc gacgagcggc acgaggacga gcgccaggag
     3961 aacaccgctg ccttcgccct ccgcggcaag atgtcccgcg aatgcgccga ccgcaaccgc
     4021 ggccccgagc acgagcgaga tcaggaagat caacatacct tttctttgtt caaagctgtt
     4081 ctgctgcatc atcgtacctc cctgttggcg ccggatcgtc agtccccggc gagacggacc
     4141 aggtcgttca ccgcctgatc gagatgaatg ggaacatcga cgacggcgac gccgcgctcc
     4201 cgcagcttga gaaacagctc cgtgaggatc ggcgggtcga tgttcgtcgc ccggagcgcg
     4261 tctacctgca tgaagatctc gttcggggtc cctgtggtga tcacgccgcg cccccgtgcg
     4321 agcacataca cgcggtcggc aaaggccgcc accaggttca gatcatgagt ggacatgatg
     4381 atggtcatgc ccgcccggtt gtaagaattg aggagcgtga ccagctccgc cctcgagcgg
     4441 ttgtcgaggc cctcgaacgg ttcatcgagg atcagaagtt ccggacgcat gaccagcgcg
     4501 ccggcaaggg ccacctttct cttctccccc ccggagagat agtggcatat cttgtcccgc
     4561 aagtgactga tcgcgagttc ttccagcacc tgttcgacca tccgttcgat ttgttccctt
     4621 ccatacccgt aatttctggg ggaaaaggag acatcgtccc agacggttgg agacaggatc
     4681 tgatcgtcga cgttctgcag gagcacgccg atgcgtttcc ggatctcgtt gtaccgctgt
     4741 gccgggttga caccgaagac ggagaccctg ccctcgtccg gcttgagcag ccccaggata
     4801 tggtagagga gcgtggtctt gcccgatccg ttccccccca ggatcgctat acgctctccg
     4861 cggttcacca cgaagtcgag cccgcagata tggatctccg tggtgtcggg gtaggtgtgc
     4921 ttaaggcagc ttacgctgat gatcgttt
//
LOCUS       DASSLD010000012         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_584615,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000012 DASSLD010000000
VERSION     DASSLD010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584615"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..336)
                     /locus_tag="VFK23_00180"
     CDS             complement(127..336)
                     /locus_tag="VFK23_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317527.1"
                     /translation="MDAAGRAEKELGAEIKVIRKTSTEYALEKNPPPRPSVIVNGRFI
                     ARNDTVTYQSLKAALLSDSDAGVTR"
     gene            complement(404..745)
                     /locus_tag="VFK23_00185"
     CDS             complement(404..745)
                     /locus_tag="VFK23_00185"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7317528.1"
                     /translation="MIPVEKRLDLLKVISHPARIKILGDLVQGVKCVSDFEETLDISQ
                     PNVSQHLKLLRSHHIVDYFIDGRLKCYFLKEPLIPDLLELLKKQYEEDVPAPACCPVT
                     KKGTYPGERRR"
     gene            989..1834
                     /locus_tag="VFK23_00190"
     CDS             989..1834
                     /locus_tag="VFK23_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET7317529.1"
                     /translation="MKKDGRDRVGVLGVKKHRFISLAALFNRLTAIIAAAFWVVSIGT
                     GASWAGPPFRTDDPEPVDYRHGEFYVATQYEKDKDVTVGTTPHFELNYGPMPDLQLHL
                     IAPFVYAKQEGEAAQRGYGDTELGVKYRFLNNEASHFMVGIFPIIELPTGNSDKGLGA
                     GHTQIFLPLWLQKAWGPWQSYGGGGYWRNPGEGNKDYWFFGWQLQRQISETLTLGAEL
                     FGNTKTEEDGKSRTGYNVGAIVNLTDDHHLLFSAGSDIGGDNRLSAYFAYQYTFGPHE
                     EKTTK"
     gene            complement(2007..2216)
                     /locus_tag="VFK23_00195"
     CDS             complement(2007..2216)
                     /locus_tag="VFK23_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258239556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2175..2177),aa:Sec)
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HET7317530.1"
                     /translation="MTETTIKIYGKMGUPFSQGARSAYGDSAEYVDVLRDKAKFKEML
                     AYTKGVSRVPVIVEGEKVTIGYGGS"
BASE COUNT          576 a          641 c          608 g          581 t
ORIGIN      
        1 cacccaaaag cggtggaacg tagagaagca gcggaatcat gaagatcgca ccgccaagcc
       61 ccaaaagccc tgagagaaat cctccgccca ggcccaatcc cacaagcgtg ctcaataatg
      121 ccgcgctcat ctcgtcaccc ctgcgtcact gtcgctcagg agcgctgcct tgagcgactg
      181 atacgtaacc gtgtcattcc tggcgatgaa tctgccgttc actatgaccg agggacgggg
      241 cggcgggttc ttctccaggg cgtactctgt cgaggtcttc ctgatcacct tgatttcggc
      301 gccaagctcc ttttccgccc gaccggcggc atccatgttc aacaatcagc gctttcccat
      361 gggatcattt acatagagcg ttatagtgcg gtccttcatc gatctatctt ctcctttcac
      421 cgggataggt tccttttttc gtcaccggac agcaggccgg cgcgggaaca tcttcttcat
      481 actgtttctt gagtaattcg agcaggtcgg ggatgagagg ctccttgagg aaatagcact
      541 tgagcctgcc gtcgatgaaa taatcgacga tatggtggga ccgcagaagc ttcaggtgct
      601 gcgacacgtt cggctgactg atgtcgaggg tctcttcgaa gtcgctcaca cactttactc
      661 cctgcacgag atctccgagg atttttatcc gggcgggatg ggagatcacc ttgagcaggt
      721 cgagacgttt ttcaacgggg atcataaaag cctccttgga gaggaataca tgcaaacatt
      781 attatgtttt tatgtttttg tcaacgtaaa agttttaatt ttatgaagat acttaggtag
      841 caggaaggtt tggttcaggc catgcgatga tgatgcaaca ctctcttctt cggaacatca
      901 ataaagccaa tggtttctcc ttgaaaccca tgcacgcctg tgctataaaa aaagttcata
      961 aatcgtttca ggataataac gagtgttcgt gaagaaggat ggtcgcgatc gcgtgggtgt
     1021 cctcggggtc aaaaagcata ggttcataag ccttgctgca ctcttcaatc gcttgacagc
     1081 gatcatcgcg gcggcttttt gggtcgtgag tatcggtacc ggcgcatcct gggctggccc
     1141 gccattccgc accgacgatc cggagcccgt cgattaccgg catggcgagt tttatgtggc
     1201 cacgcagtat gaaaaagaca aggacgtgac cgtcggcaca acgcctcatt tcgagctcaa
     1261 ctacgggccg atgcccgatc tgcagctcca tctgatcgcg ccctttgttt atgccaaaca
     1321 ggagggagag gccgcccagc gcggctacgg tgacacggaa ctcggggtca agtaccgttt
     1381 tctgaacaac gaggcgtctc actttatggt cgggatattc cccatcattg aactaccaac
     1441 cggcaacagc gacaaagggc ttggcgcggg ccatacgcag atattcctgc ctctctggct
     1501 gcagaaggcg tggggaccat ggcagagcta cggcggaggc ggttactggc gaaatcccgg
     1561 tgaagggaat aaggattact ggttcttcgg ctggcaactg cagcgtcaga tttcggaaac
     1621 attgacgctc ggcgcagagt tgttcggcaa tacgaaaaca gaggaagacg gaaaatcacg
     1681 caccggctat aatgtggggg cgatagtcaa tttgacagac gatcatcatc tcctcttctc
     1741 cgcgggaagc gacatcgggg gtgataacag gctgtctgct tacttcgcgt atcagtatac
     1801 gtttggacct catgaggaaa aaaccacaaa atagtcacgt cataatttta ccgactgttc
     1861 ctgaatcgcc gaccgtatga gagacgctca cccatcacat cgctgacccg atcgcccctt
     1921 ccttgccaaa taatattgaa gaacagcata aagaaactcg cagcgcgata aataacctta
     1981 ccgacacccg tcggtcctca tactgttcag gaacctccat aaccgatggt tactttttct
     2041 ccttcgacaa taaccgggac acgggatacg cccttcgtat atgcaagcat ttccttgaat
     2101 tttgccttgt cccttaaaac gtcaacatat tcggcagaat ctccataggc cgacctggcc
     2161 ccttgactga agggtcaccc catcttgcca tagattttga tggttgtttc cgtcatgacg
     2221 ttacctccct ttttctattg ttctcatcct gattaacccg cgtcaccgcc gggaccgcac
     2281 cgccagagcg taaaaataga agttgcagtg ggggttgcgg gtgtacgtgt actcaccacc
     2341 gctgtagaaa ttgaagtacc acgccaacga tggatctgta tccagctctg atgtccatac
     2401 aagctt
//
LOCUS       DASSLD010000013         3717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1367468, whole genome shotgun sequence.
ACCESSION   DASSLD010000013 DASSLD010000000
VERSION     DASSLD010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367468"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1843
                     /locus_tag="VFK23_00200"
     CDS             122..1843
                     /locus_tag="VFK23_00200"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HET7317531.1"
                     /translation="MRTIRISLAQINPTVGDLAGNRDKIIDCIGRARKLGADVVAFTE
                     LAITGYPPEDLLLKPQFVKDNLRTLKQITRATRGITAVVGFVDSNDRITDAAAVMHNG
                     RLVHVYHKMLLPNYGVFDEYRYFKPGKRCPIITVKGVNIGVNVCEDIWFADGPARTQA
                     AAGAEVIITINASPYHKGKANERRRMLAQRARDNNVYIVYTNTVGGQDELVFDGYSMV
                     LDPKGRLLAAGKQFEEDLITCDVALPGHFKHVDLKSKQIPDGQRDFIDVVALSTRPGT
                     PGKPLPRTGVVKPCPLCEEVYRTLVLGTRDYVNKTGFKAVVIGLSGGIDSSLVAAIAV
                     DAVGKENVFGVYMPSPYSSDESCEDASDLARNLGVKMLSIPIHQPFNAYLNTLRAAFA
                     RTKQDVTEENLQARIRGNILMALSNKFGWLVLTTGNKSEMSTGYATLYGDMAGGFAVI
                     KDVPKTMVYDVCRCLNRINGRAIIPRRVLVKPPTAELRPNQKDSDSLPVYPVLDPILK
                     AYVEDDKEFEEMLRMGFDRQTVKRVIRLVDSSEYKRRQAPPGIKITPRGLGKDRRFPI
                     TNRYRSY"
     gene            2008..2832
                     /gene="rsmI"
                     /locus_tag="VFK23_00205"
     CDS             2008..2832
                     /gene="rsmI"
                     /locus_tag="VFK23_00205"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET7317532.1"
                     /translation="MPGTLYIVSTPIGNLEDITVRAIRVLKEVDLIAAEDTRHTRHLL
                     DRYEITTPLTSYHDHNKEEKAPVLVARLLEGSSVALVSDAGTPGISDPGYFLINVAAD
                     QKIPVVPVPGATAAIAALSISGLPTDSFVFEGFLPAKHAARLKRLKNLTQEKRTIIFY
                     EAPHKIVKTLEDMIEVLGDRKAVVTRELTKIHEETIRGMFSDILSHLREGTVRGEFTI
                     VVHGASTEPAAQDIDPAEYLKNLMLHRGLSKKEAIAAAAEELGLPKKEVYKESLKL"
     gene            3076..>3717
                     /locus_tag="VFK23_00210"
     CDS             3076..>3717
                     /locus_tag="VFK23_00210"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET7317533.1"
                     /translation="MQDKRRYKRFKLDILEINGKMILAQKIEILDIGLGGVALKTDRR
                     LNVGKEYLIKLYYKGKSIDVRGVIVRCALSNIEERNGEGVVIYAAGMMFKDPSSDMFT
                     DFINSIELDKKNMELPTTDRRHNVRFTITTPFEKILSFPAQFKVKVISQSGMLIQTDQ
                     PMEIESMTPMGLTLQETDKPINFIGRVASCQKKEDRGNSHYEIGVEFKDLTDSG"
BASE COUNT          913 a         1072 c         1025 g          707 t
ORIGIN      
        1 tcaacgttga actgattaaa gacggcataa ctcgtattgc aaatggaatt taatcctagc
       61 gcgctttgcg cctttgcgag aggcgctttt gattttgatc ttataattga gtgagaaacc
      121 aatgagaaca atccgtattt cccttgccca gataaacccc accgtggggg acctggccgg
      181 caaccgggac aagatcatcg actgcatcgg acgggcgcgg aagctcggcg ccgatgtggt
      241 cgcattcacc gagcttgcga tcaccggtta tccgcccgag gacctgctcc tgaagccgca
      301 gttcgtaaag gacaacctcc ggacgctcaa gcagatcacc agggcgacgc gggggatcac
      361 agcggtggtc gggttcgtgg actcgaacga ccgcattacc gacgcggctg cggtcatgca
      421 caacggcagg cttgtccacg tttatcacaa gatgctcctg ccgaactacg gcgtcttcga
      481 cgagtaccgc tacttcaagc cgggcaagcg gtgccccatc atcacggtca agggcgtgaa
      541 tatcggcgtg aacgtctgcg aggacatctg gttcgccgac ggtcctgcgc gtacacaggc
      601 cgcggcaggc gcggaagtca tcataacgat caacgcctcg ccctatcaca agggcaaggc
      661 caacgaacgc cgccgcatgc tcgcccagcg cgcgcgggac aacaatgtct atatcgtcta
      721 caccaacacc gtcggaggtc aggacgaact cgtcttcgac ggctacagca tggtcctgga
      781 cccgaagggg aggctcctcg ctgcgggcaa gcagttcgaa gaggacctta tcacctgcga
      841 tgtcgccctt ccggggcact tcaagcatgt tgacctcaag tcaaaacaga tccctgacgg
      901 gcagcgggat ttcatcgacg tcgtggctct ctcaaccagg cccgggacgc cggggaaacc
      961 tcttccccgg acaggcgtcg taaaaccctg tcccctgtgc gaggaggttt atcgcactct
     1021 cgtcctgggg acccgcgact acgtgaacaa gaccgggttc aaggccgtcg tcatcgggct
     1081 ctcgggcggg atcgattcct ccctcgtcgc ggcgatagca gtcgacgccg tcgggaaaga
     1141 gaacgtcttc ggcgtttaca tgccgtcgcc ctacagctcg gacgaaagct gcgaggacgc
     1201 gtcggacctt gcccgcaacc tcggcgtcaa aatgctctcg atcccgattc atcaaccgtt
     1261 caacgcctac ttgaacaccc tgcgtgccgc gttcgcccgc acgaagcagg acgtgaccga
     1321 ggaaaatctg caggccagga tccgcgggaa cattctcatg gcactctcga ataagttcgg
     1381 ctggctcgtt ctgaccaccg gaaacaaaag cgagatgtcc acgggctacg ccacgctcta
     1441 cggcgacatg gcgggcggct tcgcggtgat caaggacgtg cccaagacca tggtctatga
     1501 cgtgtgcagg tgcctgaacc gcatcaatgg ccgggccatt atcccccggc gcgttctcgt
     1561 caagcccccg actgcggagc tccggccgaa ccagaaagac agcgactccc tcccggtcta
     1621 cccggtgctc gatcccatcc tcaaggccta tgtggaggac gacaaggaat tcgaagagat
     1681 gctcaggatg ggcttcgacc gccagacggt caaacgcgtc atccggctcg tggattccag
     1741 cgaatacaaa cgccgccagg ccccgcccgg catcaagatc accccgcggg ggctggggaa
     1801 agacagacgg ttccctataa cgaaccggta cagaagctat tgatcttgat aaattctgtt
     1861 ttttgaggtc gtcgttcccg cgaaagcggg aatttatgct tttcaaaagg ctaaattcaa
     1921 aacggattcc cgatagaacc attcggggat gacggtttgc gctatgagat ctttgctgca
     1981 ttcgtttaag gccagggtga ttacattatg ccaggcactc tctacatcgt ctccaccccc
     2041 atcgggaacc tcgaagacat cacagtccgg gcgatccgcg tgctcaagga agtggacctc
     2101 atcgcggccg aggacacgcg ccacaccaga cacctgctcg accgctatga gataacgacc
     2161 ccgctcacga gctaccacga ccacaacaag gaggaaaagg ccccggtcct cgtggcgcgc
     2221 ctgctcgaag ggagcagcgt ggccctcgtg tcggacgccg gcacccctgg catctccgac
     2281 ccgggctact tcctcatcaa cgtcgccgcg gaccagaaga ttcccgttgt ccccgtcccc
     2341 ggcgcgaccg cggccatcgc cgcgctgtcc atctccggcc tcccgacgga cagcttcgtg
     2401 ttcgaaggtt tccttcccgc caaacatgcg gccaggctca aacggctgaa gaatctgaca
     2461 caggagaaac gcacgatcat tttttacgag gccccgcaca agatcgtaaa gacccttgaa
     2521 gacatgattg aggttctcgg cgaccgaaag gccgtggtca cccgtgaact gaccaagatc
     2581 catgaagaga cgatccgggg catgttttcg gacatactca gccatcttcg ggaaggaacg
     2641 gtcaggggag aattcaccat cgtcgtccac ggggcttcga cggaaccggc cgcgcaggac
     2701 atcgatcccg ccgagtactt gaagaacctc atgctccatc gcggactgag caagaaggaa
     2761 gcaatcgcag cggcggcgga ggaactgggg ttgccgaaga aggaagtgta taaggagagt
     2821 ttgaaactat aatcaagatc agtcgaagac atcctcctgc agcgccaccg ccattgctat
     2881 gcctcaaggc acatgatgtt acattctgca acgcgctcaa acgctaccca taaaataccc
     2941 actcaccgct gttgacatcg cactcattaa gacatatatt tattagtccg gaacgttccc
     3001 gaagggctcc tggcaatttt caacggtgaa acgtacaacc ggaattttat acagggagag
     3061 cagaggtaac gcctgatgca ggacaagagg cgctataaga gattcaaact ggacatcttg
     3121 gaaataaacg gcaagatgat cttggcccaa aaaatcgaaa tcctcgacat cggtctcggg
     3181 ggagttgccc ttaaaacgga caggaggctg aatgtcggca aggaatattt gataaagctg
     3241 tattacaagg gaaagagcat cgacgtaagg ggcgtcatcg tccggtgcgc gttaagcaac
     3301 attgaagaga gaaacgggga gggagtcgtg atatacgcgg cgggtatgat gtttaaagat
     3361 ccctcgtcgg acatgttcac cgattttatc aattcaatcg agctggataa aaaaaacatg
     3421 gagttgccga cgaccgaccg gaggcacaat gtccggttta caattaccac tccctttgag
     3481 aagatcctga gctttcctgc gcagttcaag gtaaaagtaa tcagtcaaag cggaatgctc
     3541 atacagactg atcagcccat ggaaatagaa agcatgactc ccatggggct gactcttcag
     3601 gagaccgaca agccgatcaa ttttatcgga agagtcgctt catgccagaa gaaggaagac
     3661 cggggtaact cgcattatga aataggagtg gagttcaaag atttgacgga cagcggc
//
LOCUS       DASSLD010000014         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1400581, whole genome shotgun sequence.
ACCESSION   DASSLD010000014 DASSLD010000000
VERSION     DASSLD010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400581"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFK23_00215"
     CDS             <1..389
                     /locus_tag="VFK23_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317534.1"
                     /translation="RPAFFMLVNFFFVNTLFAFVPWGLGDIAYHIGRTSIIFYSGLLV
                     VRKNLGGVRHAAAAGVLMYFIDHVALKGGVFLLNYLFHPGGLGLAAFGSVIASFIMFI
                     PLAMLIGAAGGWYARSTMTRSSAGPQ"
     gene            complement(468..2135)
                     /locus_tag="VFK23_00220"
     CDS             complement(468..2135)
                     /locus_tag="VFK23_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7317535.1"
                     /translation="MNGRKGYVVIIGVFAIIILALIVSSIYFQRTHQMEMAEQFNRQQ
                     LLLANAHATSIQDYLGGIKNELIHIARSASQYSVRRDADFKFITDGVFRDGGVVKKQV
                     VFLDSRGTGLFTRGNAHAPGPDEKNLIMRLKTMCPADVLTQQDAKTISFIAPVCRLDS
                     LTGAVMITLDIQDIAKAFLGSGSLKAGSNENAWMMDEKGNLLYHPTKPDMVGRNLNNV
                     DSTCFSCHKSFDVERKIIQGRGDYCSGDYYGRYVAPTGEDKILAYSSAMAGDARWIVA
                     VSAPYSAITLSIKRSTNLYTWIIVLIAMTSSAVSAWLIVLNQKREQAEERAKHEKELE
                     MMHAEKLASLERLTSGITSEIGNPLTSVFSFIQVLRDMEEDEFKKETVETIFLHMNRI
                     ADILKQLSSFSKSQVLALKPCKVNTVIENSLSLIQYDKRVQDITIVKDLKSGMPAITT
                     DGSQLSQVVVNLVLNAADAMPNGGTLTIRSRVQDGGITIDFEDTGVGISKEVMGRIFD
                     PFFSTKEQGTGLGLAVSYNIIRKLGGSITAESEPGKGSRFVITLPAG"
     gene            complement(2148..>2996)
                     /locus_tag="VFK23_00225"
     CDS             complement(2148..>2996)
                     /locus_tag="VFK23_00225"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET7317536.1"
                     /translation="AAASEAPVLILGESGSGKNLVAHAIHKLSRRKDGPFIQMNCASL
                     NDQLLESELFGHKKGSFTGAVSDRVGRFEAAHEGCIFLDEIGDMPMTMQVKLLRAIED
                     KVIERVGDHTPIPVNIRLISATNKDLSNLLQTEKFREDLLYRINSIVIKVPPLRERKE
                     DIPFIALHYLRKISSINNKDIKSISPAAVDILMDFPWPGNVRRLINALEHSAATCKSD
                     VIEVTDLPDYLVYEKKAEGSERRIDQEEIRSTLSLYKGNRTLAAKHLGISRVTLWKRL
                     KALGIE"
BASE COUNT          558 a          848 c          841 g          749 t
ORIGIN      
        1 gtcggccggc ctttttcatg ctcgtcaatt ttttcttcgt gaataccctc tttgcctttg
       61 ttccctgggg cctcggagac atcgcctatc acatcggaag gacctcgatc attttttatt
      121 ctgggttgct cgtagtgagg aaaaacctgg ggggcgtacg gcatgccgct gcggcagggg
      181 tgttgatgta cttcatcgac catgtggcat taaagggcgg tgtttttctt ttgaactatc
      241 ttttccatcc cggagggttg gggctcgccg cattcggcag cgtgatcgcg tcctttatca
      301 tgttcatccc cctcgcgatg cttatcggcg ctgcaggagg atggtatgcg cgctccacaa
      361 tgacgcgatc gtccgccggt ccgcagtgag caggaacacg gctcgttgcg cgccgcgacg
      421 gtcacgtcat acaaacttca cggtaccgga ggactggccc cgctccttca tcctgccggc
      481 agggtaatca cgaaccgtga tccctttccg ggttcgcttt ccgcggtgat gctcccgccc
      541 agtttcctga taatgttgta gctcacggcg agacccaatc ctgtcccctg ctctttcgtg
      601 gagaaaaagg ggtcgaagat cctgcccata acctccttgc tgattccgac gcccgtgtcc
      661 tcgaaatcga tcgtgatgcc gccatcctga acccggctcc gaatggtgag ggtccccccg
      721 ttcggcattg cgtccgccgc attcagtaca aggttcacga cgacctggga aagctggctt
      781 ccgtccgtgg tgattgccgg catgccggat ttgaggtctt tcacaatggt aatgtcctgc
      841 actcttttgt cgtactggat gagggaaagg gaattctcaa taacggtgtt caccttgcag
      901 ggcttcaggg ccaggacctg cgacttcgag aaactggaca gttgcttcag gatgtccgcg
      961 atcctgttca tgtggagaaa gatggtctcg acggtctctt tcttgaactc atcctcttcc
     1021 atgtccctca gtacctggat aaaggaaaaa accgacgtca gcgggttccc gatttcagag
     1081 gtgatgcccg aggtgaggcg ctcaagtgat gcgagtttct cggcatgcat catctcaagc
     1141 tccttttcat gtttcgcccg ttcttccgcc tgttcccgtt tctggtttaa tacaatcagc
     1201 catgcggata cggcgctgct cgtcatggcg atcaggacga tgatccaggt atacaaattc
     1261 gtggacctct tgatcgacag agtgattgcg gaatagggag ccgatacggc cacgatccac
     1321 cgcgcatccc ctgccatcgc cgaggaatag gccaggatct tgtcctcgcc ggtcggcgcg
     1381 acgtatctgc cgtagtagtc gccgctgcaa tagtcgcccc tcccctggat gatcttccgc
     1441 tccacgtcaa aggatttatg gcaggagaaa caggtcgagt cgacattgtt cagattcctg
     1501 ccgaccatgt cgggtttcgt gggatggtac aggaggttcc ctttttcgtc catcatccag
     1561 gcattctcat tcgagcctgc cttgagggaa ccggaaccga gaaacgcttt ggcgatgtcc
     1621 tgaatgtcca gggtgatcat cacggcgccc gttaacgagt ccagccggca caccggggcg
     1681 atgaaggata tcgttttcgc gtcctgctgg gtcaggacat cggcggggca catcgttttc
     1741 agcctcatga tgaggttctt ttcatcgggc cccggggcat gcgcgtttcc ccgggtaaaa
     1801 agcccggtcc cacggctgtc cagaaacacg acctgtttct tgacgacacc gccatcacga
     1861 aaaactccgt ccgtgataaa cttgaaatcc gcgtcgcggc ggaccgagta ctgcgacgct
     1921 gatcgagcaa tatggattag ttcgtttttt attccgccca gatagtcctg gatgctcgtg
     1981 gcatgggcgt tggcaagaag cagttgctgc ctgttgaact gttccgccat ctccatctgg
     2041 tgcgtacgct gaaagtagat gctcgagacg ataagtgcca gaatgatgat cgcgaagacc
     2101 ccgatgatga ctacgtagcc cttccgaccg ttcatcgtat catctcccta ttcaataccg
     2161 agcgccttca gtctcttcca gagcgtgacc ctgctgatgc cgaggtgctt cgccgcgagt
     2221 gttctgtttc ccttgtataa ggagagggtc gagcggatct cctcttgatc gatccggcgc
     2281 tcgcttccct cggccttctt ctcatacacc aagtaatcag gaaggtccgt gacctcgatg
     2341 acgtcactct tgcaggttgc ggcgctgtgc tcaagggcgt tgatgagccg ccgcacgttg
     2401 cccggccagg gaaagtccat gaggatgtcc acggcggcag gactgatgct tttgatgtcc
     2461 ttgttgttta tcgaggaaat ttttctgagg tagtggagcg caatgaacgg gatgtcctct
     2521 ttccgttccc gaaggggcgg caccttgata acgatggagt tgatccggta caggaggtcc
     2581 tcccggaatt tctccgtctg caggaggtta ctcaggtcct tgttcgtagc cgatatgagc
     2641 cgtatgttca ccgggatcgg cgtgtggtcg ccgacgcgct cgatgacctt gtcttcgatt
     2701 gccctgagaa gctttacctg catggtcatc ggcatatcgc cgatctcgtc caggaagatg
     2761 cagccctcat gagcggcctc gaacctcccg acccggtcgc tgaccgcgcc cgtaaaggaa
     2821 ccctttttgt ggccgaagag ctcgctttcg agaagctggt cgttcaggga cgcgcagttc
     2881 atctggatga acggtccgtc cttgcgtctg ctgagtttgt ggatggcatg ggcgacgagg
     2941 ttctttccgg agccgctttc gcccagaatc aggacagggg cctcgctcgc cgcggc
//
LOCUS       DASSLD010000015         2346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_486748,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000015 DASSLD010000000
VERSION     DASSLD010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486748"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VFK23_00230"
     CDS             complement(<1..822)
                     /locus_tag="VFK23_00230"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7317537.1"
                     /translation="MRERVTMTWKQNLSRATGLMTMFCRISAPLFLRFMIAGFLAFLP
                     TTAFAAEEMLNLQALIAEALANNHDILAAETKWQAATYRIDQATTLPDPMFMIGYQNE
                     GWDKYTFGKELGAQWMYSLSQMFPFPGKRGLKGEMAARDAESNEAMYRAQRLRTVATV
                     KELYYDLFLAYKNIDIIADRTKLFSRIEDAAIARYSSGMASQQEVLMAQTEKYMLLEK
                     EAMQKQKVRSLEAMLSAAIGREANVPLGKPAEPAQTPFNASLEELQETAVRQSPEV"
     gene            complement(846..1235)
                     /locus_tag="VFK23_00235"
     CDS             complement(846..1235)
                     /locus_tag="VFK23_00235"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HET7317538.1"
                     /translation="MKKYVIFAIVLLLAAGVAYAKGYEVKKKAGEYDIVVTFDKNPPV
                     ANDNEASIAIKDASGKPVTDAKVKVDYSMPAMAGMPPMNYKAEAALQGDEYKATLGLS
                     MSGSWNIAVKITRGGKTVTAKFTVDAK"
     gene            complement(1279..1500)
                     /locus_tag="VFK23_00240"
     CDS             complement(1279..1500)
                     /locus_tag="VFK23_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317539.1"
                     /translation="MKNERGEVVTGVMVLMMVGMMIFGLFFMHGGHGNHGAGHGDTQM
                     QHSDGDHRHTDMHDNAQEKDPVPAEDGKK"
     gene            complement(1714..1836)
                     /locus_tag="VFK23_00245"
     CDS             complement(1714..1836)
                     /locus_tag="VFK23_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317540.1"
                     /translation="MKEMSQQMAKGKTDPAMTAFAGLNKGGPDFGLNFGVVRWF"
     gene            complement(1960..>2346)
                     /locus_tag="VFK23_00250"
     CDS             complement(1960..>2346)
                     /locus_tag="VFK23_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317541.1"
                     /translation="TQPMNPLVTGSGTGATQDMRNYGITTAAMSQYAKNIGMTNSSGI
                     ITDMMNDASDGIMNGMMGSTQITMGGMGGGMMGGGGTMMQATAGTSGLATAMTTFMGS
                     ITNHSGLSTTDIQALINKLAASNGTI"
BASE COUNT          460 a          672 c          594 g          620 t
ORIGIN      
        1 gacctccggg gactgccgta ccgcggtctc ctgcagttcc tccaggctcg cgttaaacgg
       61 tgtctgggcg ggctcggccg gttttcccag cgggacgttc gcttcccggc cgatggctgc
      121 ggagagcatg gcttcgaggg atcggacttt ctgcttctgc atggcctctt tttcgaggag
      181 catgtatttt tccgtctggg ccatcagcac ttcttgctgc gatgccatgc cggaggaata
      241 ccgcgcaatg gccgcgtcct cgatcctcga aaacagcttc gtcctgtcgg cgatgatgtc
      301 gatgttcttg tacgcgagga acaggtcgta ataaagctcc ttcacggtgg cgacggttct
      361 gagccgctgg gcgcggtaca tcgcctcatt gctttccgcg tcccgtgcgg ccatctcgcc
      421 cttcaggccc cgcttgcccg gaaaggggaa catttgggag agggagtaca tccactgcgc
      481 ccccagttcc ttgccgaagg tgtacttgtc ccagccttca ttctggtagc cgatcatgaa
      541 catggggtcg gggagggtcg ttgcctggtc gatgcggtac gttgcggcct gccacttcgt
      601 ctcggctgcc aggatatcat ggttgttcgc gagggcctcg gcgatgagcg cctgaagatt
      661 caacatctct tctgcggcaa aggcggtggt cggcaggaag gccaggaatc cggcaatcat
      721 gaaacggaga aagaggggag cggagatacg gcaaaacatg gtcataaggc cggtcgccct
      781 gctcagattt tgcttccacg tcatcgtcac cctttctctc actcccctat tctccgtttc
      841 atggtttatt tcgcgtctac ggtgaacttc gcagtgacgg tcttcccgcc gcgcgtgatc
      901 ttcaccgcga tgttccagga gccgctcatc gacagcccca aagtggcctt atactcgtca
      961 ccctgcagag ctgcttccgc cttgtaattc atcggcggca tgcccgccat tgccggcatg
     1021 gagtaatcca cttttacctt cgcgtcggtt actggttttc ccgaagcatc cttgatagcg
     1081 atactggctt cattgtcgtt cgcgacaggc gggttcttgt cgaaggtcac gacaatgtcg
     1141 tactcgcccg ctttcttctt tacctcgtag ccctttgcat aggctacacc tgccgccaaa
     1201 agcaatacaa ttgcaaatat aacatacttt ttcatacccg tttcctcctt tataagttta
     1261 cgtccgatgc cggtcgcgtt atttcttccc gtcttctgcg ggcacgggat ccttttcctg
     1321 tgcattgtcg tgcatatccg tatgacgatg gtcgccgtcc gagtgctgca tctgcgtatc
     1381 gccgtgtccc gcgccgtggt ttccgtgccc tccatgcatg aagaacaatc cgaagatcat
     1441 cattccgacc atcataagaa ccattacgcc ggttacgact tctccccttt cgttcttcat
     1501 tgtgtcacct ccttacaaaa tggtaactcc attgccctat aatttgcaag acaggggcca
     1561 aaaaaggcac gaaataatat cctgaaaatc aatctgttag gaaaatacgc cgatgagaga
     1621 cgcgtacgga ttgaggtgtc tgattgataa gctgtaggat atactacagt tgaagaagga
     1681 ttcgtaaggc aaacgtcaag cagtattatt gtgctagaac catctcacta cgccgaaatt
     1741 aagcccaaag tccggcccgc ccttattcaa accggcgaag gccgtcatcg cggggtccgt
     1801 tttccctttc gccatctgct gggacatttc cttcatggaa gatcatatcg ataaaagcct
     1861 atgatatacg aaggaaatat ggcagccgga tcgatctccg gctgccattg ctgttttctt
     1921 tattgatgga gcgaaattcc gctcaacaac atccgtttac tatatcgttc cgtttgacgc
     1981 agccagcttg ttgatgagag cctgtatgtc cgtggttgaa agacctgaat ggttcgttat
     2041 agaccccatg aacgttgtca ttgcagttgc caatccgctt gtccccgcag tggcctgcat
     2101 catggtgcca ccaccgccca tcatgccgcc gcccataccg cccatcgtta tctgggtgct
     2161 tcccatcatg ccgttcatga ttccatccga tgcgtcattc atcatgtcag ttataatccc
     2221 tgatgagttc gtcatcccga tgttcttcgc gtactgggac atagcggctg tcgtgatccc
     2281 gtaattcctc atgtcttgag ttgcgccggt tcccgatccg gtcaccagcg ggttcatcgg
     2341 ctgagt
//
LOCUS       DASSLD010000016         2954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1041626, whole genome shotgun sequence.
ACCESSION   DASSLD010000016 DASSLD010000000
VERSION     DASSLD010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041626"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="VFK23_00255"
     CDS             complement(<1..1453)
                     /locus_tag="VFK23_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET7317542.1"
                     /translation="MAVSAAKHKHWNEIVGLILLAAGLLISLSLISYTATDPCFSVPG
                     SGQQAKNFIGVIGAYLSDALLRLFGAAAYLIPLFLFGYGVFFAVGAEAEHPHLKRIGG
                     IVLFISSASFFSLQGETTHLFGETVPSGGMLGDLISHLFISGFSAAGSYIITVTALVV
                     SLMLLTPFSLLRMLAWLHALCERISEQTDLLVALYRGRRDKAREMKQQRLERPKEKPL
                     IVESQKQPALPQPKIVKLEKPAKAVQQSLPGMEGESAKGSYELPSPELLDPLPPATRK
                     VSKEDMLAQSELLQHKLQDFDILGRVTQVYPGPVVTMFEFEPAPGVKVSRIVNLSDDL
                     ALAMKAHSVRIVAPLPGKAAVGIEVPNNTRETVYFRQILETPEYQANKSRLKIPLGKD
                     IFGASVIASIDRMPHLLVAGATGSGKSVAINSIILALLYNARPSEVRLALVDPKMLEL
                     SMYEGIPHLLSPVVTQPKKAAETLRAIVAEMERR"
     gene            complement(1467..2276)
                     /gene="uppP"
                     /locus_tag="VFK23_00260"
     CDS             complement(1467..2276)
                     /gene="uppP"
                     /locus_tag="VFK23_00260"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HET7317543.1"
                     /translation="MILFEAIILGIIQGFTEFLPISSSAHLILLPWMFGWQGTLVDSL
                     NFDVALHAGTLVAILAFFWRDWLDLLKKFFSGLGDGTWKTGEGRLVWFIVLATVPAGV
                     LGVKYEHVVEESFRNPLLIASSLILISIVMWVADRFSAKAASLERMTFGHALFIGCAQ
                     AVALVPGVSRSGSTIIAGLLAGYTRESAARFSFLLSTPVIAGAAVLKLHKLRLAPGEA
                     LPFALGTAFSAIVGYLSIKFLLRYLTRHSLNLFVWYRLALAGAVLMLLALR"
     gene            complement(2295..2756)
                     /locus_tag="VFK23_00265"
     CDS             complement(2295..2756)
                     /locus_tag="VFK23_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317544.1"
                     /translation="MLLTILLIIHVICVIAWIGGVTFVTTVVFPMMYRTEGSLEKALL
                     FQGVEHRFAGIVKWLIGIVGITGFWLLFAKYGFAILAQPRAIGVVIMIFAWALYTTIL
                     LMERKIFGRIFADPEKIDMNKALRMINVMHWFLLTISYSAVAGGVWFGHGG"
BASE COUNT          644 a          846 c          943 g          521 t
ORIGIN      
        1 acctgcgctc catctcggcc acgatcgccc gcagagtctc ggccgccttc ttgggctggg
       61 tcacgaccgg cgagaggagg tgggggatgc cctcgtacat cgagagctcc agcatcttcg
      121 gatctaccag ggcgagcctg acctcgctcg ggcgcgcgtt gtagagaagg gcgaggatga
      181 tggagttgat ggccacgctc ttgccggagc ccgtggcgcc cgcgaccagg aggtgcggca
      241 tcctgtcgat ggaagcgatc accgaggctc cgaagatgtc cttgcccagc gggatcttga
      301 gcctcgactt gtttgcctgg tattccggcg tctcgaggat ctggcggaaa tagacggtct
      361 cgcgcgtgtt gttcggcacc tcgatgccta cggctgcctt tcccggaaga ggggcaacga
      421 tgcggaccga gtgagccttc atggcgagcg ccaggtcgtc ggagaggttc acgatccttg
      481 agaccttcac gcccggcgcg ggttcgaact cgaacatggt gaccacgggg ccggggtaga
      541 cctgggtcac cctgcccagg atgtcgaagt cctggagctt gtgctgcagg agctccgact
      601 gtgcgagcat gtcctctttt gagaccttcc tggtagccgg cggcagcggg tcgagaagct
      661 ccggcgacgg aagctcgtag gacccctttg cgctctcccc ttccatgccg ggcaggctct
      721 gctgcacggc ctttgccggt ttttccagct tgacgatctt cggctgggga agcgccggct
      781 gcttctggga ttcgacgatc aggggcttct ctttggggcg ctccagccgc tgctgcttca
      841 tctctcgcgc cttatcccgc cttccccggt agagggcgac aagcaggtcc gtctgctcgg
      901 agatccgctc gcagagagca tggagccacg ccagcatccg gagaagggaa aagggcgtga
      961 gcagcatgag cgagacgaca agggcggtca cggtgatgat gtaggagccc gccgcggaaa
     1021 aaccggagat gaacaggtgc gagatgaggt ctccgagcat gccgcccgag gggacggtct
     1081 ccccgaagag atgcgtcgtc tcgccctgga gggagaaaaa agaggccgag gagataaaga
     1141 ggacgatgcc cccgatcctc ttgagatgag ggtgttccgc ctccgctccg accgcaaaga
     1201 aaacgccgta gccgaacagg aagaggggaa tcaggtaggc ggcggcgccg aagaggcgga
     1261 gcagggcgtc ggaaagatag gcgccgataa cgccgataaa gttcttcgcc tgctgacccg
     1321 aaccggggac gctgaaacag gggtccgtcg cggtgtagga aatcaggctc aggctgatca
     1381 ggagccctgc ggcgagcagt atcagcccga cgatttcgtt ccagtgctta tgtttggctg
     1441 cggaaaccgc catacactcc ttaagattac ctcagcgcca gaagcataag gaccgcgcct
     1501 gccagggcca gccggtacca gacaaagagg ttcagggaat gccgcgtcag gtagcgaagc
     1561 aaaaatttta tgctgaggta cccgacgatt gcggagaacg ccgtccccaa ggcgaagggg
     1621 agcgcttctc ccggcgcaag acggagcttg tgcagtttca gaaccgcggc accggctatg
     1681 accggcgttg acagaagaaa ggagaatcgg gccgcgctct cgcgggtgta tcccgcgagc
     1741 agtcccgcaa tgatggtgct gcccgaccgc gatacgcccg gcaccagcgc aacggcctgc
     1801 gcgcacccga taaacagcgc gtggccgaac gtcatccgct cgagggaggc ggctttcgcg
     1861 ctgaagcggt cggccaccca catcacgatg ctgatcagga taagcgatga agctattaaa
     1921 agcgggtttc ggaagctttc ttcgacgaca tgctcgtatt tgacaccgag cactccggcg
     1981 gggaccgtcg caaggacgat gaaccacacg agccgtcctt ccccggtctt ccaggttccg
     2041 tcgccaaggc cggaaaaaaa ttttttaaga aggtcaagcc aatcccgcca gaaaaaagcc
     2101 aggatcgcca ccagggtccc ggcgtgcagg gcgacgtcga aattcaggct gtccaccagc
     2161 gtcccctgcc agccgaacat ccacggcaag agtatcaggt gggcggacga actgatgggc
     2221 agaaactccg tgaaaccttg gataatgccc agaattatcg cttcaaaaag aatcatctca
     2281 ataccccgtt cgcatcatcc gccgtgtccg aaccaaacgc cgcccgcaac ggctgaatag
     2341 ctgatcgtca ggaggaacca gtgcatcacg ttgatcatcc tgagcgcttt gttcatgtcg
     2401 atcttttccg ggtcggcgaa gatcctaccg aagatcttcc gctccatcaa cagtatcgtg
     2461 gtatacaggg cccaggcaaa gatcataatg acgacaccga tagccctggg ctgcgccagg
     2521 atcgcaaagc cgtatttcgc gaaaaggagc cagaagccgg tgatgcccac gatcccgatg
     2581 agccatttta cgatgcccgc gaagcggtgc tcgacgccct ggaacaggag cgctttttca
     2641 agggagccct cggtccggta catcatcgga aagacgacgg tggtcacgaa ggtgacaccg
     2701 ccgatccagg cgatgacgca gatgacatga atgatgagca ggatggtgag aagcattttc
     2761 ccccgaggct ggaatacctg aatattggga cattatttca caaaggaggg aggaattcaa
     2821 gatgttttga ggaggggggt gcggaggagc gggagagctg caggggcatg ccgggcactc
     2881 gataaaaaag ccgcacacgg cttcctgccg tgtgcggcgg ggaggactct tccgggggaa
     2941 cgctgaacgt ctac
//
LOCUS       DASSLD010000017         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1204817, whole genome shotgun sequence.
ACCESSION   DASSLD010000017 DASSLD010000000
VERSION     DASSLD010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204817"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="VFK23_00270"
     CDS             <1..1313
                     /locus_tag="VFK23_00270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7317545.1"
                     /translation="ADDLNAAFLEMRLSEKNYFLYHDSKALGDIRDKITSTMKTIEAE
                     KKNIIPAIGEEDFRKLETYLNSYAGVVSQARGAIPEDREIELMIRGYGKMLKEFSESV
                     TRLERKRVNDIILSSKRILIFSFWAILASALLISHFISQKIVRSLRAIERLTKSISEG
                     NFNRIEGFRTRDELGSVITAINSMSEELAHREDEIIQSKKLASLGILTAGVAHEITNP
                     LNNISMIAQTYEELYAALEEKDRVDFMRKVDAETERIRKIVKNLLDFSKPKDAHPKKV
                     DINGVIQKTLTLVQNMIDVSNIETRVNLEDGLPPLYIDEHQIQQVFVNLITNAVQSMT
                     AGGRLFITSRPGKGGESVEISVIDTGRGIPPEYLPHIFDPFFSTKGEGGTGLGLSVSY
                     GIVKNHKGEIRVESKVGVGTTFTVELPRRYDEEIRHGQIQHHDH"
     gene            1285..1650
                     /locus_tag="VFK23_00275"
     CDS             1285..1650
                     /locus_tag="VFK23_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7317546.1"
                     /translation="MDKYSIMIIDDEKIVGDMAKLSLEQEGYDVETFLNAGPALARMK
                     EKKFHVVVTDYKMKGIDGMEVLRTVKREYPETTVIMITAFANLDAAIEALRGDVHDFF
                     PKPVRIKELKESIRRALKH"
     gene            1772..>2657
                     /locus_tag="VFK23_00280"
     CDS             1772..>2657
                     /locus_tag="VFK23_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET7317547.1"
                     /translation="MNIKSIVSSLSRTFSRRTPPAGPAAFRGLFDNFKQVLESNNRAL
                     ETITELGEKLGGDYLFDIVYVRNAYATLRQHMASSLQTFNVLTRGRYPKLGETFGRID
                     SLIQQAIDEAPRAAAEPVLFLEDIPFGLVREVGGKSSQLAEVGNRLGLSVPDTFVITT
                     TGFDEFVRYNGLESPIARMKNHAAPSNAGLRDLRELILQGTIPPELGAAIGKAVDRFR
                     TRVNNTSLSVRSSAEEEDGARSFAGQFRTVLNVPLDVKQVGQAYKKVIASLFDEKAVS
                     YQVQQGYGVGKLKMAAACM"
BASE COUNT          630 a          754 c          769 g          504 t
ORIGIN      
        1 tcgccgacga cctgaacgcc gccttcctgg agatgcgcct gtcggagaag aattactttc
       61 tctatcatga cagcaaggcg ctcggcgaca tccgggacaa gatcacctcc acgatgaaga
      121 ccatcgaggc ggaaaagaaa aatatcatcc ctgccatcgg ggaagaggat ttccggaagc
      181 ttgaaaccta cctgaactcc tacgccggcg tcgtttcgca ggcccggggc gcgatcccgg
      241 aggaccggga gattgaattg atgatccggg gctacggcaa gatgctgaag gaattttccg
      301 agtccgtgac ccgcctcgag cggaaacgcg tgaacgacat catcctgagc tcgaagcgca
      361 tcctgatctt ttctttctgg gccatcctgg cctcggccct cctgatcagc catttcatat
      421 cgcagaagat cgtgcgttcg ctcagggcca tcgaacggtt gaccaagtcc atctccgagg
      481 gcaacttcaa ccgcatcgaa ggtttccgga ccagggacga actcggatcg gtcatcacgg
      541 cgatcaattc catgtccgag gaactggcgc accgcgaaga tgagatcatc cagtccaaga
      601 agcttgcgtc tctcggcatt ctcaccgccg gcgtcgctca tgagatcacg aacccgctga
      661 acaacatctc catgatcgcc cagacctacg aggagctcta tgcggctctc gaggaaaagg
      721 accgggtcga tttcatgcgg aaggtggacg cggaaacgga acggatcagg aagatcgtca
      781 agaaccttct ggatttttcc aaacccaagg acgcgcatcc gaaaaaggtg gacatcaacg
      841 gcgtgatcca gaagaccctc acgctggtcc agaacatgat cgacgtgtcg aatatcgaaa
      901 ccagggtcaa tctcgaagac ggcctgcccc ccctgtacat cgacgagcac cagatccagc
      961 aggtgttcgt caacctgatc acgaatgccg ttcagtccat gaccgcgggc ggaaggcttt
     1021 tcatcacctc gcgtcccggc aagggcggcg agtccgtgga gatatcggtc attgacacgg
     1081 gaagggggat cccgcccgag tacctgccgc acatcttcga tccctttttc agcaccaagg
     1141 gcgagggggg gacgggcctg ggactttcgg taagctacgg catcgttaaa aaccacaagg
     1201 gggagatcag ggtcgagagc aaggtggggg tcggcacgac gtttaccgtc gaattgcccc
     1261 gacgctacga tgaggagata aggcatggac aaatacagca tcatgatcat tgacgacgag
     1321 aagatcgtgg gcgacatggc gaagctttcg ctcgaacagg aaggttatga cgtcgagacg
     1381 ttcctgaacg ccgggccggc gctggcacgg atgaaggaga aaaagttcca cgtcgttgtg
     1441 accgattaca agatgaaagg catcgacggc atggaggtcc tgaggaccgt gaagcgggag
     1501 tacccggaga ccacggtcat catgatcacg gccttcgcca atcttgacgc ggctatcgag
     1561 gcgctccggg gcgatgtgca cgacttcttc ccgaagcccg tcaggatcaa ggaactgaag
     1621 gagtcgatca gaagggcgct aaagcattga tgattgtcga ttggaaaatg aggtcaactc
     1681 acaggacgca cttttcgacg atgaatttat ctttcgcttt aacagtcatc aatcaacaat
     1741 catcgatcaa caattctcta tgaaacatcc cgtgaacata aaatcgatag tatcctccct
     1801 gtcccggacg ttctcgcgca ggacgccgcc cgcaggtcct gcggcgtttc gcggcctgtt
     1861 cgacaacttc aaacaggtgc tggaaagcaa caaccgcgcg cttgaaacga tcacggagct
     1921 tggagagaaa ctcggcgggg actacctctt cgacatcgtg tatgtccgga atgcgtatgc
     1981 aaccctccgg cagcacatgg cttcttcgct ccaaaccttc aacgtcctga cccggggccg
     2041 ttatccgaag ctcggcgaga cgttcggccg gatagactcg ctcatacagc aggccatcga
     2101 cgaagcaccc cgtgcggctg cagagcctgt cctgttcctg gaagatattc ctttcgggct
     2161 ggtgcgcgag gtggggggga agagttcgca actcgccgag gtggggaacc ggctgggatt
     2221 gtccgtgccc gacaccttcg tgataaccac tacggggttc gatgagttcg tgaggtacaa
     2281 cggactggaa agccctatcg cgcggatgaa gaatcacgcc gctccttcga acgccggcct
     2341 ccgtgacctc cgggaactca tcctgcaggg gaccattcct cccgaactcg gcgcagccat
     2401 cggcaaggca gtggacaggt tcagaacccg cgtgaacaac acgtctctgt ctgttcgaag
     2461 cagcgccgag gaagaggacg gcgcccgttc ctttgccggc cagttccgga ccgtactgaa
     2521 tgtcccgctc gacgtgaagc aggtgggaca ggcttacaag aaggtcattg cgagcctgtt
     2581 tgacgagaag gccgtctctt atcaggtcca gcagggatac ggcgtcggaa agctcaaaat
     2641 ggccgccgcc tgcatgg
//
LOCUS       DASSLD010000018         3333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_486374,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000018 DASSLD010000000
VERSION     DASSLD010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486374"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFK23_00285"
     CDS             <1..270
                     /locus_tag="VFK23_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317548.1"
                     /translation="SDGVKILALVDAFDAMTTDRPYRQKLGLDETFREVIKCCGTQFD
                     ERITRTFFNLLLRELSGEVKDPQILPLLGQAQTVKLTAFRPGADM"
     gene            413..1027
                     /locus_tag="VFK23_00290"
     CDS             413..1027
                     /locus_tag="VFK23_00290"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET7317549.1"
                     /translation="MKRIVNNYTEFLELTTEQFREATKKNKVKCRKGCHECCASGFFD
                     ITLLDALHLRESLKKLPPDIRKRVVDKANEQLDILEKKGAFSRKDPLLKTLPAIDSIS
                     RRSAKMRCPALDDKGACLIYDHRPHICRIFGPTVRGKRRAVRLEGCGYFKNEIPEADF
                     PIMSHYADEDVLLKAMFVKAGRKRIREVDTIIPAALTLDLERWL"
     gene            1076..1624
                     /locus_tag="VFK23_00295"
     CDS             1076..1624
                     /locus_tag="VFK23_00295"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7317550.1"
                     /translation="MEIIDKKIVWKGKFMSAVEITYRDAKGVLRTWEALERVGIGGIV
                     VMVAVTPAGNVILEKQFRPPVGREVLELPAGLVEPGESMEVAAKRELIEETGWSAGRL
                     EFLAEGPISTGASTEALRAYLCTELVFVGKNGGDDNEIIEVIEVPLGHAQEYLHHAQS
                     KGTLIDLKVFGLVELARKALGL"
     gene            complement(1646..2158)
                     /locus_tag="VFK23_00300"
     CDS             complement(1646..2158)
                     /locus_tag="VFK23_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317551.1"
                     /translation="MPDTGEGTSGREDQARCLVVSLEPAVSRLAATGGREIPFTRAVL
                     TMRNACPAELVNIVPHASLGQEGGTVQDVSGAFPGSAVASIPPGGTVSWDVYDLLLPA
                     HPGTASKVHMFGYRAALNWRFDLVAWAEYRRSADSPPVRTALSRWVLRWSVPDSSTGA
                     VALAIEEGKD"
     gene            complement(2229..>3333)
                     /locus_tag="VFK23_00305"
     CDS             complement(2229..>3333)
                     /locus_tag="VFK23_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM/SPASM family putative metalloenzyme
                     maturase"
                     /protein_id="HET7317552.1"
                     /translation="GESLLHPQLETFIARARALLPESASVGFQSNGMALSEERAASLV
                     DAGLDRICISVDAVSDDGFRSIRSGGEMEGIVAAFAGLNKARARARKRDLAIGIEFVL
                     MRDNITDLPEAIRWAGRSGAGFAIVSQLLPYHKDLVAQTVYDTNTTAAIAVYERWKAK
                     ALREGLNIRRYLDTITKVGRTPEEERIVQLVEQMRNDAAAEGIALHIERLLRRDDEWL
                     ASAERILDEARKAAREEGMDLTLPGMAPRNTRKCEFVEGGGAFVSWDGNVHPCYFLWH
                     RYACYVGGVAKRVKPWIFGNVRDRGLLAIWNDGASRSFREHVLGYQFPFCFDCGFALC
                     DYVSEEEFEQDCYLGQVPCGACLWCTGLFHCLS"
BASE COUNT          681 a         1035 c         1023 g          594 t
ORIGIN      
        1 agtgacggag tcaagatcct ggcgctcgtc gatgccttcg acgccatgac tacggaccgc
       61 ccgtaccgcc agaagctggg gctcgacgaa acattcaggg aagtcattaa atgctgcggc
      121 acgcaattcg acgagcggat cacgagaaca tttttcaacc tgctgctcag ggaactgagc
      181 ggcgaggtca aggaccctca gatccttccc cttctgggac aggcgcagac ggtcaagctc
      241 acggcttttc ggccaggggc agacatgtag ctttcaacgc cccctattcc gaaattttcc
      301 cgcccccggc atcataacgc tccggactgt cattatcttc acttcatatt gacaaacaac
      361 ccgccgagcc cgtataatcc tcggacctcc cggaggaaag gcgacggtgt ccatgaaaag
      421 aatcgtaaac aattacacgg aattcctcga gctcactacg gagcagttcc gtgaagcgac
      481 aaaaaagaac aaggtgaaat gcaggaaagg ctgtcatgag tgctgcgcgt cgggcttttt
      541 cgacatcacg ctcctggacg cccttcacct gcgggagagc cttaaaaaac tcccgccgga
      601 catcaggaag cgcgtggtgg acaaggcgaa cgagcaactg gacatcctgg agaaaaaggg
      661 cgccttctcc cgcaaagacc ccctcctcaa gacgctcccg gcgatcgact ccatctcccg
      721 ccggtcggcc aagatgcgct gtcccgcact ggacgacaaa ggcgcctgtc tgatctacga
      781 ccaccgcccc cacatctgcc gcatcttcgg accgacggtc cgcggcaaga ggcgcgcggt
      841 gaggcttgag ggctgcggct atttcaagaa cgagatcccc gaggcggatt ttcctatcat
      901 gagccactat gcggatgagg acgtgctgct caaggccatg ttcgtcaagg ccggccgcaa
      961 acgtatccgc gaggtggata ctatcatccc tgcggcattg acgttggacc tggagaggtg
     1021 gctgtaataa agcaatgcgg aattcggatt gcggaattcg gaatgaactg atgccatgga
     1081 gataatcgac aagaaaatcg tctggaaggg caagttcatg agcgccgtgg aaatcaccta
     1141 ccgcgacgcg aagggagtgc tccgcacctg ggaggccctg gagcgggtgg ggatcggggg
     1201 catagtggtg atggtcgcgg tcacgccggc gggcaacgta atcctcgaaa aacagttccg
     1261 gccgcccgtc ggccgggagg tgctggagct tcccgcgggg ctcgtggagc cgggcgagtc
     1321 catggaggtc gccgcgaagc gtgaactcat cgaggagacg ggctggtccg ccggcaggct
     1381 cgaatttctc gccgaggggc ccatatccac cggcgcctcg accgaggcgc tccgcgccta
     1441 cctgtgcacc gagcttgtct ttgtcgggaa gaacggggga gacgacaacg agataataga
     1501 ggtcatcgag gtccccctcg ggcatgccca ggaatacctg caccacgccc agtcaaaggg
     1561 cacgctcatc gacctcaagg tgttcgggct cgtggaactg gcgagaaaag cgttgggact
     1621 gtaacggcga ggatgtttga tgtcgtcagt ccttcccctc ctcgatcgca agcgctacgg
     1681 cgccggtgga ggaatcgggg acgctccatc gcagcaccca ccgtgacagg gctgtccgga
     1741 caggagggga atcggcagag cggcggtatt ccgcccatgc aacaagatcg aacctccagt
     1801 tcagtgccgc gcgatatccg aacatgtgga ccttgctcgc ggtcccggga tgtgcaggaa
     1861 gcaggaggtc gtacacgtcc cagctgacgg tgccgccggg cgggatcgat gcaacggccg
     1921 agccggggaa ggcgccggag acatcctgga ccgtgccgcc ctcctggccg agcgatgcgt
     1981 gcgggacgat gttgaccagc tccgcagggc aggcattgcg cattgtcagg accgccctcg
     2041 taaaggggat ctccctcccg ccagtcgctg ccagacgtga aacggcaggc tccaatgaga
     2101 caacgaggca gcgtgcctgg tcctctcttc ctgaagttcc ctccccggtg tccggcatgg
     2161 ctcgtacctc ctctcgcagc agtcttgtcc ttagtatatc acggcttggt gcgcggcaac
     2221 ttcaccgctc acgagaggca gtggaacagg ccggtacacc agagacaggc tccgcagggg
     2281 acctgtccga gatagcagtc ctgctcgaac tcttcctcgc tcacatagtc gcacagcgcg
     2341 aagccgcagt cgaagcagaa ggggaactgg tagccgagca cgtgctcccg gaaggaacgg
     2401 gaggccccgt cgttccagat ggcaaggagg cccctgtcgc ggacgttgcc gaagatccag
     2461 ggtttgaccc gtttggcgac gccgccgaca taacaggcgt aacggtgcca gaggaagtag
     2521 cagggatgca cgttgccgtc ccaggagacg aaggcgccgc ccccttcgac gaactcgcac
     2581 ttcctcgtgt tccgcggcgc catgcccgga agcgtcaggt ccatcccctc ttcccgggca
     2641 gccttccggg cctcgtccaa tatccgttcg gccgacgcaa gccactcgtc atcccgtctg
     2701 agcagcctct cgatatgcag ggcgattccc tccgctgccg cgtcgttcct catctgttcg
     2761 acaagctgta cgatccgctc ttcttccggg gtccttccta ccttcgtgat ggtatcaaga
     2821 taccgccgga tgttcaggcc ttcccgcagc gcctttgctt tccaacgctc gtagacggca
     2881 atggccgcgg ttgtattcgt atcgtaaact gtttgcgcca cgagatcctt gtggtaggga
     2941 aggagctggg agacgatggc gaagccggct cctgacctgc ccgcccagcg gatcgcttcg
     3001 ggaagatcgg tgatgttgtc gcgcatcaag acgaactcga tcccgatcgc gagatcgcgc
     3061 ttcctggccc gcgccctggc cttgttcagg cccgcgaagg ccgcgacgat gccctccatc
     3121 tccccgccgc tccggatgga acggaagccg tcgtcggaca ccgcgtcgac cgagatgcag
     3181 atgcggtcga gcccggcgtc gacgagcgac gcggcacgct cttcgctcag cgccataccg
     3241 ttcgattgga atcccacgga tgcgctttcc ggaaggaggg cgcgcgcacg ggcaatgaac
     3301 gtctccaact gagggtgaag cagggactcg ccg
//
LOCUS       DASSLD010000019         2420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_30131,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000019 DASSLD010000000
VERSION     DASSLD010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30131"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VFK23_00310"
     CDS             <1..778
                     /locus_tag="VFK23_00310"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET7317553.1"
                     /translation="TFVQPGDEIVSADPSFVVYKMITQAACGANVIVPCKDLCHDLDA
                     MAERITPKTKIVFIANPNNPTGTMNTRAEMDRFMSRVPDHVIVAVDEAYFEYVTHAEY
                     PDSLDYLKEGKNVLALRTFSKIYGLAGLRIGYGITKPEIAELMNKVRQPFNTNSLAQA
                     AALAALSDRKHVEKSIAINNEGKQYLYQSFQRLGVQYIPTEANFILFETAIDAKDVYA
                     ALLKQGVIIRPMGGKGLRVTIGLPEENKRFVAELEKIVKA"
     gene            873..1886
                     /gene="aroF"
                     /locus_tag="VFK23_00315"
     CDS             873..1886
                     /gene="aroF"
                     /locus_tag="VFK23_00315"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET7317554.1"
                     /translation="MIIVVKPDATEEQVDHIIKKIKDLGLQAHVSKGVERTIIGAIGD
                     EAILQSVPLEAIPGVEKVLPILKPFKLASWEFKREKTVITVNGQQIGGARIAVMAGPC
                     AVENKTLLIEIARDVKAAGATFIRGGAFKPRTSPYAFQGLEEEGLKYLAEARKAVGLP
                     IVTEVMDPRDIGLIAGYADVLQIGARNMQNFRLLREVGLCKKPVLLKRGISATIKEWL
                     MSAEYIMSGGNHQVILCERGIRTYETATRNTLDLSAVPVLKQMTHLPVIVDPSHAVGK
                     WDLVAPMAKAAVAAGADGLIIEVHSNPEEALCDGEQSLKPKSFKQLMDEMKPIAAALG
                     REI"
     gene            1959..>2420
                     /locus_tag="VFK23_00320"
     CDS             1959..>2420
                     /locus_tag="VFK23_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HET7317555.1"
                     /translation="MVLHFNKVTIIGVGLIGGSLARVLKERGLATQITGAGRSRETLE
                     LALRLGVIDHMGQGMSHAVENADLVVLASPVGVFEAVVREFASHLKKGSIVTDVGSVK
                     GGLVRAIEAILPAGVHYVPGHPIAGKEKHGVAESSGALFVEAKCILTPTEKT"
BASE COUNT          550 a          713 c          743 g          414 t
ORIGIN      
        1 cacctttgtc cagcccggag atgaaatcgt ctccgcagat ccttccttcg tcgtgtacaa
       61 gatgattacc caggccgcct gcggcgctaa cgtcatcgtg ccctgcaagg acctctgcca
      121 cgacctcgac gcgatggccg agcggatcac gccgaagacg aagatcgtat tcatcgccaa
      181 tccgaacaat cccaccggga ccatgaacac ccgcgcggag atggaccggt tcatgagccg
      241 cgttccggac cacgtgatcg tcgcggtcga tgaggcgtat ttcgagtacg tcacccacgc
      301 ggaatatccc gattccctgg actatctgaa agaaggcaaa aacgtactcg ccctccgcac
      361 cttttcgaaa atctacggcc ttgccggcct ccggatcggc tacggcatca cgaaacccga
      421 gatcgcggaa ctcatgaaca aggtgaggca gccgttcaat accaactcgc tggcgcaggc
      481 ggccgccctg gcggcgcttt cggaccgcaa gcatgtcgag aaatccatcg caatcaacaa
      541 cgagggcaaa cagtacctct atcagtcctt ccagcggctt ggcgtccagt acatcccgac
      601 agaggccaac ttcatcctct tcgaaaccgc catcgacgca aaagacgtct atgccgcgct
      661 cctcaaacag ggggtgatca tccggcccat gggcggaaaa gggctcaggg tcacgatcgg
      721 cctccccgag gagaacaagc gcttcgtggc ggagctggaa aaaatcgtaa aggcgtgaag
      781 cgcaaagtgc atggcgcata gcgcatggcg taagagacgt taacgctatg cgcttcgcgc
      841 tctgcgctgt gcgtttttca gaaaggaaag tcatgatcat cgttgtgaaa ccggatgcga
      901 ccgaggagca ggtcgatcac atcataaaaa agatcaagga cctggggctc caggctcacg
      961 tgtcgaaggg cgtcgaaagg acgatcatcg gcgccatcgg ggacgaggcg atcctccaga
     1021 gcgtgcctct cgaagcgatt cccggcgtgg aaaaggtgct ccccatcctg aaacctttca
     1081 agctggcgag ctgggaattc aagagggaaa aaaccgtgat caccgtcaac ggccagcaga
     1141 tcggcggcgc gcggatcgcg gtcatggcgg gcccctgcgc cgtcgagaac aagacgctcc
     1201 tgatcgagat cgccagggac gtgaaggccg cgggcgcaac gttcatccgc ggcggcgcgt
     1261 tcaaacccag aacttcgccc tatgccttcc agggtcttga agaggaaggg ctcaagtatc
     1321 ttgccgaggc gcgcaaggcc gtggggcttc ccatcgtgac ggaggtcatg gacccgcgcg
     1381 acatcggact gatcgccgga tacgcggacg tgctccagat cggcgccagg aacatgcaga
     1441 acttccggct gctcagggag gtggggttat gcaagaagcc cgtgctcttg aagcgcggca
     1501 tatcggccac gatcaaggaa tggctcatgt ccgccgagta catcatgtcc ggcggcaacc
     1561 accaggtgat cctctgcgaa cggggcatcc ggacctacga gacggcgacc cggaacacac
     1621 ttgacctctc cgccgtaccg gtcctcaagc agatgacgca tcttccggtc atcgtggacc
     1681 cgagccatgc cgtgggcaaa tgggacctcg tagcccccat ggcaaaggcc gcggtcgccg
     1741 ccggtgccga cggcctgatc atcgaagtgc attcgaaccc cgaggaagcg ctctgcgacg
     1801 gcgaacaatc cctcaagccg aagagcttca agcaactgat ggatgagatg aagccgattg
     1861 cggcggcgct gggaagggag atataacagt tcaaagttca aagttcggag ttcggagaaa
     1921 cggcaactgc agggttcaac ggtgtaatct gaaaaatcat ggtacttcac ttcaataagg
     1981 taacgatcat cggcgtcggt ctcatcggcg gttcgctggc tagggtcctg aaggagcgcg
     2041 ggctcgccac gcagatcacg ggcgcgggca ggagcaggga gacgctcgaa ctggccctta
     2101 ggctgggggt aatcgaccac atgggacagg gcatgagtca cgccgtcgaa aacgcggacc
     2161 tcgtggtcct ggcatcgccc gtgggcgtct tcgaagcggt cgtccgcgag ttcgcttcgc
     2221 acctgaagaa gggatcgatc gtgaccgatg tgggcagcgt caaagggggc ctcgtgcgcg
     2281 ccatcgaagc cattcttccg gcgggcgtgc actacgtgcc cgggcatccc atcgcgggca
     2341 aggagaaaca tggcgtggcc gagtcgtcgg gggcgctgtt tgtggaggcg aagtgtattc
     2401 tgactccgac ggaaaaaacg
//
LOCUS       DASSLD010000020         2288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_682493,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000020 DASSLD010000000
VERSION     DASSLD010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682493"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFK23_00325"
     CDS             <1..327
                     /locus_tag="VFK23_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7317556.1"
                     /translation="QKWYLEVGQGMEKTQRIADEKRAYTLTDRGTWLATKDKDKLDMI
                     IVLEGDPALFNQYGVMAVNPEKAKTAKYKEAMEFVNWVISKEGQDAVAAFKDKNGNPL
                     FIPNAK"
     gene            442..1146
                     /locus_tag="VFK23_00330"
     CDS             442..1146
                     /locus_tag="VFK23_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7317557.1"
                     /translation="MDSILNALSRAFTLILHLNPELIGIILLSLKVSGLALIIATVLG
                     LPAGAALGLTKLPGRGLAVSTVNTFMGLPPVVVGLFVYLLLSRKGPLGFMGLLYSPTA
                     MVIAQTILAVPIVTALCHSAIASVDPLIRQAARTLGATPLQETTTIIREARYGILSAV
                     IAGFGRVMAEVGSILIVGGNIAGYTRVMTTTIALETDKGNFELALALGIILLAISFIV
                     NAALHAVQRKGAVPVL"
     gene            1143..1799
                     /locus_tag="VFK23_00335"
     CDS             1143..1799
                     /locus_tag="VFK23_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7317558.1"
                     /translation="MKLIASHIRKSYGGKIILRDCSVSFEGMGPYVLMGPNGCGKSTF
                     LRICSLLENPDNGDLSFISGGLPLPQDLALRRRITMVLARTGVFNTTVFRNVAYGLKI
                     RGERQDEIEKKVNQALAFVGLKNMAGQRAVTLSSGETQRLGLARALVTEPDILFLDEP
                     TASVDEENTGIIESIIRTMKEGGRTTVIMTTHDRDQAERLADVLLLMKDGMIEAQGER
                     "
     gene            complement(1878..>2288)
                     /locus_tag="VFK23_00340"
     CDS             complement(1878..>2288)
                     /locus_tag="VFK23_00340"
                     /inference="COORDINATES: protein motif:HMM:NF040826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HET7317559.1"
                     /translation="NGCGAVYMNKRWYAENGKSAIAKKEKVQLTCPACLKIRDNFPGG
                     IVTLKGDYVLPHKADLMNLIRNEEERARGLNPLERVIAIKENGFGSIVISTTNEKLAQ
                     RIGRAIKKAFHGDVAYHWSHDNKLVRVDWVREMG"
BASE COUNT          474 a          706 c          625 g          483 t
ORIGIN      
        1 cagaaatggt atcttgaggt cggccagggc atggagaaga cccagcgcat cgctgatgag
       61 aagcgggctt acacgcttac cgaccgcggg acctggctcg ccacgaagga caaggacaag
      121 ctggacatga tcattgtgct cgaaggcgat ccggcgctct tcaaccagta cggtgtaatg
      181 gccgtgaacc ctgaaaaagc aaagacggca aaatataaag aagcgatgga attcgtcaac
      241 tgggtgatct cgaaggaagg ccaggacgct gtcgctgctt tcaaggacaa gaacggcaac
      301 ccgctgttta tcccgaacgc gaagtaggta ggattgaaac acgatgcagg atacattgtg
      361 catgataagt gccgcattat atccggcatc ttacatccta catcctgaaa ctttcagctt
      421 gcatcatgca tcgaggtccc catggactcc attctgaacg cactaagccg ggcatttacc
      481 ctgatcctcc acttgaatcc tgagctcatc ggcatcatcc tgctgtcgct caaggtgtcc
      541 ggcctcgccc tgatcatcgc gactgttctc ggtctgccgg caggggcggc actcggcctc
      601 acaaagctcc cgggccgcgg ccttgccgtc agcaccgtga ataccttcat ggggctcccg
      661 cccgtcgtcg tgggtctttt cgtctatctt ctcctttcac gaaaaggccc gctcggcttc
      721 atgggtttgc tgtactcgcc gacagccatg gtcatcgccc agactatcct cgccgtgccg
      781 atcgtgaccg ctctctgtca ctcggccatc gcaagcgtcg atccgctcat caggcaggcg
      841 gcccgcaccc tcggcgcaac gccgctgcag gaaacaacga cgattatccg cgaggcccgc
      901 tacggcatcc tttcagccgt catcgcgggc ttcggcaggg tcatggccga ggtgggatcg
      961 atcctgatcg tcggcggaaa cattgcaggc tacacccggg taatgaccac caccatcgcc
     1021 ctcgaaacgg acaagggcaa cttcgagctg gccctcgccc tcggcatcat tcttctggcg
     1081 atctccttca tcgtgaacgc ggcgctccac gccgtccaga gaaagggggc ggtgccggtc
     1141 ctgtgaaact catcgcctcc cacatcagaa aatcatacgg cggcaagatc attctcaggg
     1201 actgctccgt ctcgttcgag ggcatggggc cctatgtgct catgggcccc aacggctgcg
     1261 gcaagtccac gttcctgcgc atctgttccc tgctggaaaa tcccgacaac ggcgacttgt
     1321 cgtttatttc cggcgggttg ccgctcccgc aggaccttgc gctccggaga cggataacca
     1381 tggtcctcgc gaggaccggc gtgttcaaca cgaccgtttt ccggaacgtc gcctacgggc
     1441 tcaaaatccg cggggagcgt caggatgaga ttgaaaagaa ggtgaatcag gccctcgcct
     1501 tcgtcggctt gaagaatatg gcgggccaac gggccgtgac cctttcgagc ggggagaccc
     1561 agcgccttgg gctggccagg gcgctcgtga cggagcccga tatccttttc ctcgacgaac
     1621 ccactgcctc ggtggacgag gagaacaccg ggatcatcga gtccatcatc aggacgatga
     1681 aagaaggcgg ccgcacgacc gtcatcatga ccacccatga ccgcgaccag gccgagcgcc
     1741 tggcggacgt gctgcttctc atgaaggacg ggatgattga agcgcagggg gaaaggtagc
     1801 acgatcaaca aaaaaggccg atttcgtatt cgaaatcagc cctctcgtcc ttcttcttca
     1861 ctcgtgaaaa tctaacccta acccatctct ctcacccaat cgacgcgcac cagtttgttg
     1921 tcgtgcgacc agtgatacgc cacatcgccg tggaacgcct tcttgatcgc ccggccaatc
     1981 cgctgagcaa gtttctcatt cgtcgttgag atgacgatgc tcccgaaacc gttttccttg
     2041 attgcgatga cgcgctccag cggattgagt ccgcgggccc gctcctcttc gttccggatg
     2101 aggttcatca ggtccgcttt gtgcgggagc acataatcgc ctttcagcgt tacgatgccg
     2161 cccgggaaat tgtcgcgaat cttgagacag gccgggcacg tcagttgaac tttttccttt
     2221 ttggcaattg ccgattttcc attctccgca taccatcgtt tgttcatgta tacggcgccg
     2281 cacccgtt
//
LOCUS       DASSLD010000021         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1465138, whole genome shotgun sequence.
ACCESSION   DASSLD010000021 DASSLD010000000
VERSION     DASSLD010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465138"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFK23_00345"
     CDS             complement(<1..188)
                     /locus_tag="VFK23_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317560.1"
                     /translation="MIEIIAGIGVAVFIIYAIFSIVYMMSMKRMNDALHDFILRTEGN
                     ANATLAELKVTIENIRKIT"
     gene            454..1101
                     /locus_tag="VFK23_00350"
     CDS             454..1101
                     /locus_tag="VFK23_00350"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET7317561.1"
                     /translation="MKNIRKGAQNGLSRRLHFPDDEKRLPWLPLLLDAYAIADTGVAV
                     AVRDEEKRRKSKLACSKGCGSCCAHQADLPFYPHELVGIYWYVSEKMVSPGRDILRNR
                     LAAHAAGSACPFLIDNSCSIHPLRPLGCRQFNVFTKPCAEGEDPYYTRREDVLVPLQD
                     YTDRAFATVLPFYNLKREADLAAAVRLIRSQIMNLQTYDWKKLVEIMEKADSRRV"
     gene            complement(1272..2318)
                     /locus_tag="VFK23_00355"
     CDS             complement(1272..2318)
                     /locus_tag="VFK23_00355"
                     /inference="COORDINATES: protein motif:HMM:NF044557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317562.1"
                     /translation="MDADLLADIKFNCDVSDAQFWGFYSVCGLLMRYRDLFRSEQGLK
                     PWADIRREDIAAWIERKEVRWPELEQKDLRPLVVDDHSFDPFDVEGINRAIDGRGLVY
                     GAGYGMYMKPSFFLAERRSVRRISGLTVFTSGKELVRDLFSSPAMLQEKTVFIRLEPL
                     ILLLLYKHSELNTRQVTALEDAFSHYGIPHRQIIDSTFELRIAEMAERYAEILLAHEL
                     AEDAEEVPDWKEVISLSAGDRQAEHYLRAVKDLVADTSDRGPLRKIIEDRDRGALGLT
                     IALTEGYRRILYPEIKAAYRDLSLHHDWTAVERARRTGYERFRSRRDEIVAMFRSGKG
                     KEEFMGEVKKLISV"
BASE COUNT          574 a          725 c          704 g          602 t
ORIGIN      
        1 gttattttcc ggatattctc aattgtcact ttgagttcag ccagcgtagc gtttgcgttg
       61 ccttccgtcc gcaggataaa atcatgcagt gcatcgttca ttcttttcat actcatcatg
      121 tagacaatgg aaaaaatagc atatatgata aataccgcca cgccgatgcc tgcgataatt
      181 tcaatcataa aacctccttt tgattaagta tagcacaggt gcgcccccgg aatgcagggg
      241 tgaacagacc ctgtttcgtg taaaaactat cctttttcag ggaaatatgc aaggtatatt
      301 ccatcccgtc gattcgagtg cgggaatatg gagcgcgaag ctttgaccgg aaaaatttaa
      361 tgcggtgcgg catattgttt tttccccggg gctgtgttat tgtgcagtgc cttttttatt
      421 gcggtcatgc ttcgtgcttg gaaggggttt taagtgaaaa atatccggaa gggggcccaa
      481 aacggccttt cgagacggct tcatttcccg gatgatgaaa aacggctgcc ctggcttccg
      541 ctcctcctcg acgcctatgc catcgcggac actggagtcg ccgttgccgt acgggatgaa
      601 gaaaagcgga gaaaatcgaa actcgcatgc tccaagggct gcggcagctg ctgcgcccat
      661 caggcggacc tgccattcta cccccacgag ctggtcggca tttactggta tgtctcggag
      721 aaaatggttt cacccggcag ggacattctg cggaatcgcc ttgcagccca tgcggccggt
      781 tcggcctgtc cgttcctgat cgacaactcc tgctccatac atccgctcag gcccctcggg
      841 tgcagacagt tcaacgtctt cacgaagccc tgcgccgagg gagaggaccc ctactacacg
      901 agaagggagg atgtgctcgt tcctctccag gactatacag accgggcctt cgccacggtc
      961 cttcccttct acaacttgaa acgggaagcc gaccttgcgg cggctgtcag actcatccgc
     1021 tctcagatca tgaacctgca aacctatgac tggaaaaagc tggtcgagat aatggagaag
     1081 gcggacagcc ggagggtgtg atggaattct agcgcaagcc cttttccttc tggtgtcatt
     1141 ccggcttgac ccggaatcca tttttcgaac ttggattccc gcttcagcgt ggcccgtttc
     1201 atcggccatc gcgggaatga caacgaatgg cagagcagga aaaggtgaag ggatgtgttg
     1261 gaaattcttc cctaaacgct tatcagcttt tttacctccc ccatgaactc ctctttcccc
     1321 ttaccgctgc ggaacatcgc gacgatctca tcccgcctgg agcggaaccg ttcgtagccc
     1381 gtcctccggg cccgttccac ggcggtccag tcatgatgaa gggaaaggtc ccgataggcc
     1441 gccttgatct cgggataaag gattctccgg tacccctccg tgagtgctat cgtgaggccg
     1501 agcgcaccac ggtcgcggtc ctcgatgatt ttcctgagcg gcccccggtc ggatgtgtcc
     1561 gcgacgaggt ctttcacggc cctgagatag tgctccgcct gccggtcgcc ggcggacagg
     1621 gaaatgactt ccttccagtc cgggacctct tcggcgtcct ccgcaagctc gtgggccagc
     1681 agaatttcag catagcgctc cgccatctcg gcaatccgca gttcaaacgt gctgtcgatg
     1741 atctgccggt gggggatgcc gtagtgcgag aacgcgtctt cgagcgcggt aacctggcgg
     1801 gtgttcaatt cggagtgttt gtacaggagg agcaggatga gcggttcgag gcgaatgaac
     1861 acggtttttt cctggagcat cgccggggag gagaagaggt cccgtacgag ttccttgccc
     1921 gaggtgaata cggtcaagcc cgagatccgg cgaacggaac gcctttccgc aagaaaaaat
     1981 gagggtttca tgtacatccc gtagccggcg ccgtatacga gaccgcggcc gtcgatcgcc
     2041 cggttgatgc cctctacgtc gaagggatcg aacgagtgat cgtcgacgac caggggcctt
     2101 aagtccttct gctccagctc gggccagcgg acctccttcc gttctatcca cgccgcgatg
     2161 tcctcgcgcc ggatgtcggc ccagggcttg aggccctgct ccgaccggaa gaggtcccgg
     2221 taccgcatga gcaggccgca gaccgaataa aagccccaga actgcgcgtc cgaaacgtcg
     2281 cagttgaatt tgatgtcagc gagaaggtcg gcatccatgg cttttccccc tgtatcgttt
     2341 aatgcattat tgctcatcgc cggtaaaaag taaatacgaa cagatctcgt atctgcccag
     2401 tcgtcattcc ggcgaaagag gaaacccggg aattttacaa aaataaaccc gaaacctctg
     2461 gattcccgat agaagcattc gggaatgacg gattggtttt acgctctttg caggcatttg
     2521 tgatcaacct gggtagttgc cagaccgttc cttgccaaaa aacccttgca actgctgaaa
     2581 aaataaacta taatttcaaa tcctc
//
LOCUS       DASSLD010000022         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_62097,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000022 DASSLD010000000
VERSION     DASSLD010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62097"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /gene="folP"
                     /locus_tag="VFK23_00360"
     CDS             <1..1122
                     /gene="folP"
                     /locus_tag="VFK23_00360"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET7317563.1"
                     /translation="GHGTFSLTVNGLTSAQMDVLHGIVRASYSSTSGAGLGPAPSFSP
                     SGAQQGFTISGSPELLRRIARNLPSGSCLPSGLAEKIESLLDNYLRSDYKIDCRGKTL
                     GLGARTHIMGVLNVTPDSFSDGGRYADPDQALARARDMAAAGADIIDIGGESTRPGAE
                     PVPEDEELRRTVPLIERLAAELAVPISIDTYKSSVARKALEAGASIVNDISGLRFSPD
                     MARVAAEYGAAVVIMHIKGTPKNMQQNPVYADVVDEVRQYLEEGVELAVKGGVDRERI
                     MIDPGIGFGKNLGHNLEILNRLDEFKGAGRPVVLGTSRKKFIGTVLDIPVPEDRGYGT
                     AATVALGIARGAAIVRVHDVADMAQVARMTDAIVKSVHS"
     gene            1276..2073
                     /gene="cdaA"
                     /locus_tag="VFK23_00365"
     CDS             1276..2073
                     /gene="cdaA"
                     /locus_tag="VFK23_00365"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HET7317564.1"
                     /translation="MNNFAEIREVLGHYRWFNDTVDIALVYYLFYRLLLLIKGTRAFQ
                     MLIGIGIILLVLLASQFLEFYTLDWLIHSFWSQIVLALVVLFQPEIRRTLARVGERRL
                     FRSMSAVEGSKFIEETVKAAVSMANKRIGALIVLERDTDLSTIVEMGTELDAKVTKEI
                     LVSIFLPYSPIHDGAAIIRNGRIVAAGCFLPLTLSSNLSKALGTRHRAAVGLTEEADA
                     IVVVVSEETGEISVVSNGVIEHNADAPALRKTLSDIFLPKKNGKLPL"
BASE COUNT          448 a          686 c          626 g          427 t
ORIGIN      
        1 ggccatggaa ccttctccct gacggtgaac ggcctgacgt ccgcacagat ggacgtcctc
       61 cacggcatcg tcagggcatc atattcatca accagcgggg caggtctcgg acctgccccg
      121 tctttttccc cctccggcgc gcagcagggc ttcacgatct ccggttcccc ggaactgctg
      181 cggcgcatcg cgcgcaacct cccgtccggg agctgcctcc cctccgggct cgcggaaaaa
      241 atcgaatctc tgcttgacaa ctatctgcgg tcggactata aaattgactg cagagggaaa
      301 acgctcggcc tcggcgcccg cacgcatatc atgggggtcc tgaacgtgac tcccgattcc
      361 ttttccgacg gtggacggta cgccgacccg gaccaggccc tcgcccgcgc gcgggacatg
      421 gcggctgccg gcgcggacat catcgatatc ggcggtgagt ccacccgtcc gggcgccgag
      481 ccggtgccgg aggacgaaga gctgcggagg accgtccccc tcatcgaacg gctcgccgcc
      541 gagctggccg tccccatttc catagacacc tacaaatcat cggtcgcgag aaaagcgctc
      601 gaggccggcg cctcgatcgt caacgacatc agcgggctcc ggttctcgcc cgacatggcg
      661 cgcgtagctg ccgaatacgg ggccgcggtc gtcattatgc acatcaaggg aacgccgaag
      721 aacatgcagc agaaccccgt ctatgccgac gtggtggatg aggttcgtca gtatctcgaa
      781 gaaggcgtcg aactcgccgt caagggcggc gtagaccggg aaaggatcat gatcgacccc
      841 ggcatcggct tcggcaagaa tctcggtcac aacctcgaga tactgaaccg cctcgacgag
      901 ttcaagggtg ccggcaggcc cgtcgtgctg gggacatcga gaaaaaaatt catcggcacg
      961 gtcctcgaca tccccgtgcc cgaagatcgt ggatacggga ctgctgccac cgtagccctg
     1021 gggatcgcgc gaggcgccgc aatcgtgagg gtccatgacg tggcggacat ggcgcaggtg
     1081 gcccggatga cggatgcgat cgtaaaatca gttcatagtt aataactcag agtactacag
     1141 cgacgacact tgtttgtcat tcccgcaaaa gcgggaatcc attttgacgt ttgttgagaa
     1201 agccaatgga tacctgcttt cgcaggtatg acaggctaaa cggaaacgct tgaactctgg
     1261 attatcctta aaatcatgaa taacttcgcc gaaatacgag aggtcctcgg gcactaccgg
     1321 tggttcaacg atacggtgga catagcgctc gtctactacc tcttttaccg gctgcttctc
     1381 ctcatcaagg gcacgcgcgc gttccagatg ctcatcggca tcggcatcat ccttctggtg
     1441 ctcctcgcgt cgcagtttct cgagttctac accctcgact ggctcatcca cagcttctgg
     1501 tcccagatcg ttcttgccct cgtggtcctc ttccagcctg agatccggcg caccctggcc
     1561 cgggtcggag aacgccgcct tttccggtcg atgtctgccg tcgagggctc caagttcatc
     1621 gaggaaacgg tcaaggcggc ggtttccatg gcgaacaagc ggatcggggc gctcatcgtt
     1681 ctcgagcgcg atacggacct ctcgacgatc gtggaaatgg gtaccgaact cgacgccaag
     1741 gtcaccaagg agatcctcgt aagcatcttc ctgccctatt ccccgatcca cgacggggca
     1801 gcgatcatca gaaacgggag gatcgtcgcc gcgggctgtt ttctgccgct cacgctctca
     1861 tcgaaccttt ccaaggcgct cggcacgcgg caccgggccg ccgtcggcct caccgaagaa
     1921 gccgacgcta tcgtcgtcgt cgtgtccgag gaaacaggcg agatttccgt cgtcagcaac
     1981 ggtgtcatcg aacacaatgc cgacgcaccc gcgctcagaa agacgctgtc ggatatcttt
     2041 ttgccgaaga aaaacgggaa acttccgcta tgaacatccg catgatattg ttcgacaact
     2101 ggggcatcaa gcttctgtcg ctgggtctgt cgctcaccct ctggtttttc gtcacgtcga
     2161 aggggaagac cgagataacg ctcacgg
//
LOCUS       DASSLD010000023         3188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1236323, whole genome shotgun sequence.
ACCESSION   DASSLD010000023 DASSLD010000000
VERSION     DASSLD010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236323"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /gene="ttcA"
                     /locus_tag="VFK23_00370"
     CDS             complement(<1..721)
                     /gene="ttcA"
                     /locus_tag="VFK23_00370"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HET7317565.1"
                     /translation="MTELDRLEHDIRRLAGRAIGDFGLIENRDRILVALSGGKDSFTL
                     LHILESLQKRAPVAFSLVAVTVHPGFPGFQTKLVEDYLKTRGVEHRVVHAPVHKLMLE
                     KLTPDDTPCALCARLKRGVLYTQARNLGCTKIALGHHRDDFIETLLLNQFYNGRIKAM
                     APLLRADDGRNVVIRPLVYVPEDDIIRFAALAGFPIACCACPACGDEDLKRVRIKNLL
                     SDLERERPGVKASLLAALADVD"
     gene            complement(861..1187)
                     /locus_tag="VFK23_00375"
     CDS             complement(861..1187)
                     /locus_tag="VFK23_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317566.1"
                     /translation="MAIERIMQYECKVCGRKIDVTRTGDVYEEPVYCCGLEATEINVP
                     VAPSAPAVPVRKPAPKKAAKKVAKKKAAKKAKKKAVKKKATKKKAAKKKATKKAAKKK
                     ATKKRR"
     gene            complement(1368..1559)
                     /locus_tag="VFK23_00380"
     CDS             complement(1368..1559)
                     /locus_tag="VFK23_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317567.1"
                     /translation="MDLDDVILELYRLAETKAWEGKTTDEALSAAIEGMEQIWEMRQA
                     DRRRTAMVNANKKQPRVWC"
     gene            complement(1725..>3188)
                     /locus_tag="VFK23_00385"
     CDS             complement(1725..>3188)
                     /locus_tag="VFK23_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HET7317568.1"
                     /translation="SINLARMLAPGGREIDFDKLRHTVRQAVHFLDNVIDANRYPIEK
                     ISQMTRANRKIGLGVMGFADMLLEMGIPYNSDEAVRTGEKVMKFITDEGRKASEELAA
                     TRGAFPNFKGSIYDSPGAAPVRNATVTTIAPTGTLSIIAGCSSGIEPLFGLTFVRKVL
                     DGAKLLEVNPVFERTAKNGGFYSDKLMKEIAEHGGCTNIQTVPEDVQKVFVTAHDITP
                     EWHIKMQAAFQKYTDNAVSKTVNFPHAATQKDVEKVYLLAYKTGCKGVTIYRDGSRDE
                     QVLNTGATEKARKAAATSHGAVTVASASPELAEEKKPRNRPVLTHGVTQKIPTGCGNL
                     YITINEDEEGICEVFSTMGKSGGCAASQSEAVSRMVSLALRSGVSLESIIKQVKGIRC
                     PSPAWGEGGSILSCPDAIGRALERYAKEGTNHRHHHKPRAAASPMPTVAYTSSDCVKG
                     DNKNHLGLCPECPDCGGLLEFGEGCAFCRGCGFSKCG"
BASE COUNT          553 a          976 c          945 g          714 t
ORIGIN      
        1 aatcgacatc ggccagagcc gccaggagac ttgccttgac cccgggccgc tcccgctcca
       61 ggtccgacag gaggttcttg atcctgaccc ttttgaggtc ctcgtccccg caggccggac
      121 aggcgcagca ggctatggga aaacccgcca gagcggcgaa tcggataatg tcgtcctcgg
      181 gaacgtaaac gagcggccgg ataacgacgt ttcttccgtc atcggcccgg agcagcggtg
      241 ccatggcctt gatcctgccg ttgtaaaact ggttcaatag gagtgtctcg ataaagtcat
      301 cccggtgatg gccgagggct atctttgtgc agccaaggtt ccgcgcctgg gtatagagca
      361 caccgcgctt gagccgtgcg cagagcgcgc agggcgtgtc gtcgggcgtc agtttctcga
      421 gcatgagctt atggaccggg gcatggacga cccggtgctc gacgcctcgg gtcttgaggt
      481 aatcctcgac cagcttggtc tggaacccgg ggaaccccgg atggaccgtt actgcaacga
      541 gtgagaacgc cacgggagcg cgcttttgca ggctctccag gatgtggagc agcgtaaagc
      601 tgtccttgcc gcccgagagc gccacgagga tgcggtcgcg gttttcaatc agaccgaagt
      661 ccccgatcgc cctgcccgca agccgccgta tgtcgtgctc cagccggtca agttcggtca
      721 tgggctcaat cctaacccgg ataagccgga attgcaaatt gtaaattgcc cataaccggt
      781 ccgatcggcc agcccaggaa tcgaaaatga aaaagggctc ccggacaatg ccggaagccc
      841 ctgggaaagc aaggagaata ttaccttctt ttctttgttg ccttcttttt cgccgccttc
      901 ttggtcgcct tcttcttagc ggcttttttc ttggtcgcct tctttttcac cgccttcttc
      961 ttcgcctttt tcgctgcttt ctttttcgcc acctttttcg cggccttctt cggcgccggt
     1021 ttcctcacgg gaacggctgg cgcggaaggc gccacaggca cattgatttc ggtagcttcc
     1081 aggccgcagc agtatacggg ctcctcgtac acatcgcctg tcctcgtcac gtcgatcttt
     1141 cttccgcaca ccttgcactc gtactgcatg atcctctcaa tagccatagg cgttgccctc
     1201 cttgataagc tgatgacccg ctgcttggcg gggggaataa cgctgcggcc cgggaaagaa
     1261 atccggcctg gtcctgcgag agctgcgatc aacgtgctcg aaagcatccg ttcactgcta
     1321 ctgtagagtc atagccctca aatgtcaagt tttttgtgca tgaatcttca acaccacacc
     1381 cgcggctgct tcttgtttgc attgaccatc gcggtccttc tcctgtcggc ctgcctcatt
     1441 tcccagatct gctccatgcc ctcgatcgcc gctgagagtg cttcatcggt tgtcttccct
     1501 tcccaggcct tcgtctctgc aagccggtag agctccagaa tcacgtcgtc tagatccatg
     1561 cagcctcctt aaagaaactt tggacgcaga tttacgccga taaacgccga tgaagaccgg
     1621 gaaatccgtc tgaacaccaa acagcgtgcc tggcgcttca aggtacaaaa aatcgatccg
     1681 cgtgcatctg cgtaaatctg cgtccggaat tcagtcttga ttcgttagcc gcatttcgaa
     1741 aatccgcagc cccggcagaa ggcgcagccc tcgccgaact ccaggagccc gccgcagtcg
     1801 gggcactcgg ggcaaaggcc caggtggttc ttgttatctc ccttgacgca gtcagacgac
     1861 gtgtatgcga ccgtgggcat cggcgaagcc gcggcccggg gcttgtggtg atgacggtgg
     1921 tttgtcccct ccttggcgta gcgttcgagg gcccggccga tcgcatcggg acaggacagg
     1981 atcgagccgc cctcccccca ggccggggac gggcagcgta tgcccttgac ttgcttgatg
     2041 atcgactcaa gggacacgcc ggaccggagc gcaagcgaca ccatgcggga gacggcttcg
     2101 gactggcttg cggcgcaccc gccggacttg cccatggtcg agaagacctc gcagatgccc
     2161 tcctcgtcct cgttgatcgt tatgtagaga ttgccgcagc ccgtggggat cttctgggtc
     2221 accccgtggg tcaggaccgg acgatttcgc ggttttttct cctctgccag ctcgggagac
     2281 gcggaggcga ccgtgaccgc gccgtgggac gttgccgccg ccttgcgggc cttttccgtc
     2341 gcgccggtat taagcacctg ctcgtcgcgg gatccgtcgc ggtagatcgt gacgcccttg
     2401 cagccggtct tgtaggccag gaggtagacc ttctccacgt ccttctgcgt ggccgcatgc
     2461 gggaaattca cggtcttcga cacggcgttg tcggtgtatt tctggaaggc ggcctgcatc
     2521 ttgatgtgcc actcgggcgt gatgtcgtgg gccgtgacaa agaccttctg cacgtcctcg
     2581 gggaccgtct gaatgttcgt acatccgccg tgctcggcga tctccttcat gagcttgtcg
     2641 gagtagaagc ccccgttctt cgccgtccgt tcgaacacgg ggttcacttc aagaagtttg
     2701 gcgccgtcca gtaccttccg gacgaaggtg agtccgaaga gcggctcgat gcccgaggag
     2761 cagccggcga tgatggaaag ggtgcccgtc ggggcgatcg tcgtcacggt cgcgttgcgg
     2821 accggggccg cgccggggct gtcgtagatg ctccctttga agttcgggaa ggccccgcgt
     2881 gtggcggcga gttcctcgct cgccttccgg ccttcgtcgg tgatgaactt catgaccttt
     2941 tcaccggtcc tcaccgcctc gtctgagttg tagggaatgc ccatctcgag gagcatgtcg
     3001 gcgaagccca tgacgcccag gccgatcttt cggttcgccc gcgtcatctg ggaaattttt
     3061 tcgatgggat agcggttggc gtcgatcacg ttgtccagga agtggaccgc ctgccgaacg
     3121 gtgtggcgga gcttgtcgaa atcgatctcc ctgccgcccg gggcaagcat cctggccagg
     3181 ttgatcga
//
LOCUS       DASSLD010000024         2962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_387922,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000024 DASSLD010000000
VERSION     DASSLD010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387922"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1549
                     /locus_tag="VFK23_00390"
     CDS             <1..1549
                     /locus_tag="VFK23_00390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7317569.1"
                     /translation="TGAALPVMLPRERYFLSFCIIGIFLCGVIIFRVLSEQKYAEQSQ
                     RETEVTLRAVTDAANEAIVMVNAGSTVTFWNPAAEKIFGYCSNEAIGNELHALLAPPR
                     YHDAYKSGFSRFQESGEGAAIGRTIELTALRKDGVEIPIELSLSALNLKGIWHAVGVI
                     RDITERKKSDEALRMHREQLENLVMERTRELNATNELLRKEITDHTRTEEELYRSESF
                     LNTIFDSFREPFSIVDRDYTIIKFNDAFARTKCKRVRDLFGKRCYEVLRGRDSVCEDC
                     IVEKTFQSKDPCTKEKLRTRPDGAEAWSEIFTYPIFDQNHNVSHVVEYSRDITARKKG
                     EDEKKELIRNLNLLSTTDGLTGLLNRRALNDILRHEIDRASRYNTDLSLIICDIDMFK
                     AINDSYGHTAGDRALQLVAETLKSALRKADIPGRYGGDEFMVILPETPLSGAQSLAEK
                     IRFAVFERALALPGNDRVRLSLSLGVSSRCSPLDDLDTLVALADTALYSAKQSGRNRV
                     SVMQPSA"
     gene            complement(1552..2058)
                     /locus_tag="VFK23_00395"
     CDS             complement(1552..2058)
                     /locus_tag="VFK23_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317570.1"
                     /translation="MKIYLKTALLVFAVVAIAFVLFIMKEASKPESSIIAGERDRLAR
                     SVDEDRAKKDEDYSRDLENKLQFLGYRLALAYIAENKADEAIAVLDKMIQDEEAMSRS
                     GRLRRSRSYFDEANYYDALIGAYQLKRDDARAIEAARTHDELMARAWELKRQEERGEG
                     RSVGLNAQ"
     gene            complement(2083..2553)
                     /locus_tag="VFK23_00400"
     CDS             complement(2083..2553)
                     /locus_tag="VFK23_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7317571.1"
                     /translation="MIKLLIAIDDSSSAMKAVDYVARQLTGCGAQIGLVHVLPNLPAI
                     FWDEGHILSEEEKGDRKKVVDKWLDAQKAKMEPILKKSMETLAEKGVPRDWMETRFVS
                     DSTDVADSILETARDGGYQTIVVGRREQPSKHILGSVSSKIINQGNGMTVIVVE"
     gene            complement(2702..>2962)
                     /locus_tag="VFK23_00405"
     CDS             complement(2702..>2962)
                     /locus_tag="VFK23_00405"
                     /inference="COORDINATES: protein motif:HMM:NF017485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HET7317572.1"
                     /translation="GDEIRPSDTVEEKVSLVDRFGLRFGFYHFSQDTYLEIVAFYAAK
                     YGVRMEKNRLEELSIRWALGAGGRNGRIAEQFVRAVQGNGAS"
BASE COUNT          641 a          880 c          811 g          630 t
ORIGIN      
        1 aaccggggcc gcgttgcctg tcatgttacc ccgggaacgc tattttctgt cgttctgcat
       61 tatcggcatt ttcctgtgcg gcgtcatcat cttccgggta ctctcggaac agaaatatgc
      121 ggagcagtcg cagagagaga cagaggtcac tttgcgcgcc gttaccgatg cagcgaatga
      181 agcaatcgtc atggtgaatg ctggcagcac tgttaccttc tggaaccccg cggcggaaaa
      241 aatattcggc tattgctcga acgaggccat cggcaatgaa ctgcatgcgc tgctggctcc
      301 gccgcgatat cacgacgcgt ataaatccgg tttcagcagg tttcaggaat cgggtgaggg
      361 cgccgcgatc ggcagaacca tcgaactgac ggccctgaga aaggacggtg tcgagatccc
      421 catcgagctt tccctgtccg ctttgaacct gaagggcata tggcatgccg taggcgtcat
      481 tcgtgacatt acggaacgca agaaatcgga cgaggcgctg cggatgcacc gggaacaact
      541 tgaaaacctg gtcatggaac ggacgaggga attgaacgca accaacgaac tcctgcgcaa
      601 ggagatcacg gaccacactc gcaccgagga agagctgtac cggtcggaaa gcttcctcaa
      661 taccatcttc gacagcttcc gcgagccgtt cagcatcgtg gaccgcgact atacgatcat
      721 aaaattcaac gatgccttcg cccggacaaa gtgcaaacgg gtgagggacc tgttcgggaa
      781 acggtgctac gaggtgttga ggggaaggga cagcgtctgc gaggactgca tcgtcgagaa
      841 aacattccag tcaaaggacc cctgcacaaa agagaagctg cggacgaggc cggacggcgc
      901 tgaggcatgg agcgaaatct tcacctatcc gatcttcgac cagaaccaca acgtatcgca
      961 cgtggtcgaa tattcccggg acatcaccgc acggaagaag ggcgaagacg agaagaaaga
     1021 gctcatccgg aacctgaacc tgctgtcgac gacggacggc ctgacgggac tgttgaacag
     1081 aagggcgctc aacgatatcc tgaggcacga gatcgaccgg gcgtcccggt acaataccga
     1141 tctttccctg atcatatgcg atatcgacat gttcaaggcc atcaacgact cctacggtca
     1201 cacggccggt gaccgggccc tgcaactggt cgccgaaacg ctgaaaagcg ccctccgcaa
     1261 ggcggacatc ccgggccgat acggaggcga tgagttcatg gtcatactcc cggaaacacc
     1321 cctctccggg gctcaaagcc tggcggaaaa gatccgcttc gccgtcttcg agcgggcgct
     1381 ggcactgccg gggaatgacc gcgtccggct gtcgctcagc ctcggtgtgt cgagccggtg
     1441 ctctcccctg gacgatctcg acacccttgt ggccctcgcc gacacagccc tttactccgc
     1501 taagcagtcg gggaggaaca gggtatccgt aatgcagcca tcagcctgaa gtcactgcgc
     1561 gttcagaccg accgatctgc cctccccccg ttcttcctgt ctcttgagct cccaggccct
     1621 ggccatcagc tcgtcgtggg tcctcgccgc ctcgatcgcc ctggcgtcgt ccctcttcaa
     1681 ttgataggcc ccgatcagcg catcgtaata gttcgcttcg tcgaaataac tgcgggagcg
     1741 ccggagtctg ccgctccggg acatcgcctc ttcatcctgg atcattttat ccaggacggc
     1801 tatggcttcg tcggctttgt tttccgcaat gtatgcgagg gccagccggt agccgaggaa
     1861 ctgcaatttg ttctccaggt cgcggctgta atcttcatcc ttttttgcgc ggtcttcgtc
     1921 cacggaacgg gcaagcctgt ctcgttctcc ggcaatgatc gagctctcgg gcttggaggc
     1981 ttctttcatg atgaacagga caaatgcgat cgcgacaaca gcgaaaacaa ggagtgctgt
     2041 tttcaaatag atcttcatat ccgttccctg attccggacc ttctattcga cgacgattac
     2101 ggtcatgccg tttccctgat tgatgatctt gctcgacacg ctccccaaaa tgtgctttga
     2161 gggctgttca cgccgtccga caacgatggt ctggtacccg ccgtccctgg ccgtttcgag
     2221 gatactgtcg gcaacgtccg tcgaatcgga cacgaatcgc gtctccatcc aatcccgggg
     2281 gacccccttc tcagccagcg tttccatgga tttttttaga attggctcca tcttggcttt
     2341 ctgtgcgtcg agccatttgt ccacgacctt cttccggtcg cccttttctt cctcggaaag
     2401 gatatgtccc tcgtcccaga agatggctgg caggttcggc agaacatgca caaggccgat
     2461 ctgcgcgccg catccggtaa gctgccgcgc aacatagtcc accgccttca ttgcgctcga
     2521 tgaatcgtcg attgctataa gaagctttat catttttctt cctccgatgg tatgttgacg
     2581 aaattaaggc gcccgcagga tatcgcgcag ttgcgggatg cggaaatcaa tataatcagg
     2641 ataatcgaaa accggtgaaa aggcaagacc gcgtgcgctg aaaagcagcg ctgccggttc
     2701 gttagctcgc gccgtttccc tggacagccc ggacgaactg ttcggcgatc cgtccgttcc
     2761 ttcccccggc gcccagggcc caccggatcg acaactcctc gagccgattc ttctccatac
     2821 ggaccccgta ttttgccgca tagaatgcga cgatctcaag ataggtgtcc tgggagaaat
     2881 ggtagaagcc gaaccgcagg ccgaatcggt ccacgagtga aaccttttcc tcgaccgtgt
     2941 ccgaaggccg gatctcgtcc cc
//
LOCUS       DASSLD010000025         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_420805,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000025 DASSLD010000000
VERSION     DASSLD010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420805"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(667..954)
                     /locus_tag="VFK23_00410"
     CDS             complement(667..954)
                     /locus_tag="VFK23_00410"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HET7317573.1"
                     /translation="MATFIMTGRYSTEAVKRISGERTTKATDIVQQCGGKLVAAYATM
                     GKTDLLVIVEFPGVGEAIRASVALNKALGIAFSTVPAIRVEEFDKLVGIKA"
     gene            complement(1126..2745)
                     /locus_tag="VFK23_00415"
     CDS             complement(1126..2745)
                     /locus_tag="VFK23_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HET7317574.1"
                     /translation="MTESYNMHLDRNPANYAPLSPLSFLAKAAYTYPQRVATIHGQER
                     WTWAQVYTRCRRLASSLSRHGIRKGDTVAAMLPNVPAMFELHFGPAMIGAVLNTLNTR
                     LDAEAIAFMLEHGESKVLFTDREFFPVVDKALKMLSKRLLVIDVDDPLCRDGNSFGMA
                     SYESFLEEGDENFEWSLPVDEWDAITLNYTSGTTGNPKGVVYHHRGAYLNAVSNILGW
                     DMGRHPVYLWTLPMFHCNGWCFPWTIAASAGTNVCLRKVDVKLIFDLIREHKVSHYCG
                     APIVHNSLINAPDEMKAGIAHKVSCYIAGAAPPVAVIEGMERMGFHLTHVYGLTETYG
                     PAAICAKHEEWDRMSLEERARLNSRQGVAYPMEEGMTVMDPKTMRPVPWDGTTMGEIM
                     FRGNIVMKGYLKNPAATDEAFEGGWFHSGDLAVMEPDGYAKIKDRSKDIIISGGENIS
                     SIELEDVLYRHPAVLGVAVVAYPDEKWGEVPFAFVELREGATVSEEEMTAFCRERMAR
                     FKSPKKFIFGPLPKTSTGKILKYVLRKKAKE"
BASE COUNT          611 a          959 c          894 g          664 t
ORIGIN      
        1 cgacgtctcc accttcgcct tcaccttctc gatttcgaca tccaactcct taagccgggg
       61 ctccattttt ttcagatatt cctggaacat aacgtccctc ctttcccagg gattgtccca
      121 accccctgtt tcgaacatgg atccctgcag acatattgtt ttgattcccc ggtttccatc
      181 aaggtttcca cctaggtttc ggtctggaat ccatctgcgc agttgcagcg tacgatcaag
      241 aggcccttga tgcccttcta aacctcgacg gggaggagga gttcgtcatc tacctggcac
      301 cggtcggcaa acacgctata tccgcgtaaa aggaaaacac ggaaattcgt cccccccttg
      361 acgccgctga aaccagtacc acgcggaggg gttgccgttt tcttccccca tcgtcgtccg
      421 gactttggag aaatccgctc cagcgctttt ccgggcgggg tccgggcgat gaattccatc
      481 acctcgggcg gagtgagttg agtgtcccaa ggggtctgcc ggcgccaata gcgccactgg
      541 aatttccatc cgttgggcag gagggatacg atccgatcat gccacatctc cggacccccc
      601 ccatcccgga tcggtctgac cgccatgacc acaacaccga ccaataggac ggcggagggt
      661 caattgctac gccttgatgc cgaccagctt gtcgaattcc tcgacccgga tagcaggcac
      721 ggtggaaaag gcaatcccca gggccttgtt caaagcgaca gaagctctta tcgcttcgcc
      781 gacgccagga aattcaacga tcaccaacag gtcggtcttc cccatcgtgg cgtatgcagc
      841 cacgagcttt ccaccgcact gctggacgat atcggtcgcc ttcgtggtgc gctcaccgct
      901 gatccgcttg accgcctcag tcgaatatct ccccgtcatg ataaatgtcg ccatgacaat
      961 ttcctcctta ctcgttttcc ttatcgtaca gggtccatta tccactatcg gcctgatgca
     1021 ggggatacgg agattatgcc gaagggggaa cccgatcccc ggcggattga atgcccaccc
     1081 ggaggctgcg gacggcatgg cgaggccggg ctggaggata gcgtcttatt cctttgcctt
     1141 cttgcgcaac acatacttca gaatcttccc cgtggacgtt ttaggcaaag gcccgaagat
     1201 gaactttttg ggggacttga atcgtgccat tcgttcccgg cagaaggcgg tcatctcctc
     1261 ctcgctcacc gtcgcacctt ccctgagctc cacgaacgcg aacgggactt cgccccactt
     1321 ctcatccggg taggcaacga cagcgactcc caacaccgcc ggatggcggt acagcacatc
     1381 ctccagctcg atggaggaaa tgttctctcc gccggaaatg atgatgtcct tggaccggtc
     1441 cttgatcttg gcatagccgt cgggttccat gaccgcgagg tcccccgagt ggaaccatcc
     1501 tccctcgaac gcttcatccg tcgccgcggg gttcttgagg taccccttca tgacgatgtt
     1561 tccccggaac atgatttcgc ccatggttgt tccatcccac ggcaccggtc tcatggtctt
     1621 tgggtccatc accgtcatcc cttcctccat cggataggcc acgccctggc ggctgttgag
     1681 gcgggcgcgt tcctcgagag acatcctgtc ccattcctcg tgcttcgcgc agatggccgc
     1741 cgggccgtat gtttcggtga gaccatagac gtgggtgagg tggaacccca tgcgctccat
     1801 tccctcgatc accgcgacgg gaggagcggc cccggcgatg tagcaggaca ccttgtgcgc
     1861 aatgcccgct ttcatctcgt cgggcgcgtt gatcagggaa ttgtggacga tgggcgctcc
     1921 gcagtagtgg gagactttgt gctcgcggat gagatcgaaa atcagcttga cgtcgacctt
     1981 gcgcaggcac acattcgttc cggccgatgc cgcgatggtc caggggaaac accacccgtt
     2041 gcagtggaac atgggcaagg tccacaagta tacggggtgc cgccccatat cccagcccag
     2101 gatgttggag acggcgttga ggtaggcgcc gcgatggtgg tagaccacgc ccttcggatt
     2161 ccccgtcgtg ccggaggtgt aattgagcgt gatcgcatcc cactcgtcga cgggcagcga
     2221 ccattcgaag ttctcgtctc cctcctccaa aaacgattcg tagctcgcca tgccgaagga
     2281 attcccgtcc cggcagagcg ggtcgtccac gtcgattaca agcaggcgct tcgacagcat
     2341 cttcagcgcc ttgtcgacga cggggaaaaa ctcccggtcc gtaaacagca ccttggactc
     2401 gccgtgctcc agcatgaacg cgatggcctc cgcgtcgagg cgggtattca gtgtgttcag
     2461 gacggccccg atcatcgcgg ggccgaagtg caactcgaac atcgccggta cgttcgggag
     2521 catggcagcg accgtgtcgc ccttccggat tccgtggcgc gagagggagg acgccagccg
     2581 gcggcaccgg gtgtagacct gcgcccatgt ccagcgctct tgaccgtgga tggtggcgac
     2641 gcgttgcgga taggtgtacg ccgccttcgc gagaaaggac aacggagata gcggcgcgta
     2701 gttggccggg ttcctgtcca gatgcatgtt gtacgattcg gtcatggctg ctccttgtcg
     2761 aatcatgtca aacgcctgaa atccgtgtaa tgccccgcag aggaaggtcg cccgcgacag
     2821 ttcgccggca tgcgcgcgat ccggaagagc taagggcgtg tctgtgaggg aagttaccat
     2881 actgtaaagg aaatcccgcg tcaatagcgt ccacgcaaaa cccctttttc atcctcaaaa
     2941 agcgagttgt ttgcgcgggg tttgcagtcc gtcccgttta tagcctgaag ccagcagggc
     3001 caccagggaa tgtgccggtg caagcaatgg ctaccgtacc ggatggcaac cgtggcagag
     3061 atccggggcg ctcttcacga gaagtcggtc gttggaagac tggtggggca ggtggcaggc
     3121 aaggcagg
//
LOCUS       DASSLD010000026         4296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1137565, whole genome shotgun sequence.
ACCESSION   DASSLD010000026 DASSLD010000000
VERSION     DASSLD010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137565"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFK23_00420"
     CDS             <1..302
                     /locus_tag="VFK23_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HET7317575.1"
                     /translation="EGMRRIGHFEFVWANLVPIAVQVAGNVFILGGLTCYVVSVAVWL
                     LVLPRVEVSYAYPLLSVGYIVNAVAGYYLFQENLSLTRITGILIICVGVYFVTKS"
     gene            437..1606
                     /locus_tag="VFK23_00425"
     CDS             437..1606
                     /locus_tag="VFK23_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016209576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HET7317576.1"
                     /translation="MSNNPNEDFLPFSRPSISQEAIDEVVACIKSGWITTGPRVKRFE
                     EDLKAYLGAPHVLALTSATAGLHLVLVALGLKPGDEVITTPMTFAATLNTIVLAGGKP
                     VLVDVEPGTYNMDVTKVEKAVTPRTRAIMPVHFAGLPVDLDPLYDIAKKHNLRVIEDA
                     AHAIGTEYKGLPIGGFGDIQVFSFHPNKNMTTGEGGCVATRDERLTSNVALLRFHGMD
                     REAWDRFGKKGNQHYEIIAPGYKYNMTDIQAALGLHQLKALDGFIRRRTELAARYQRL
                     LQDWPQWALPATPPYKHRHAWHLYTPLINPEAAGMDRDAFMQGMKERNIGTGLHYHAV
                     HLYRYYRKHFGFKHGDFPHAETISDRIVSLPLFPAMTDADQDRVVAAMEDLFGRK"
     gene            1609..2550
                     /locus_tag="VFK23_00430"
     CDS             1609..2550
                     /locus_tag="VFK23_00430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006035777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7317577.1"
                     /translation="MAKPSISVVVPVYNEQENLEALFTRLTTVMDKTGKPYEILFTND
                     GSRDRSGEMLRDFHKRRPKQVRVIDFNGNFGQHMAIMAAFERARGEVIVTLDADLQNP
                     PEEIPKLLEAVESGHDVVGGYRRNRQDTFFRKYASKVINGIRAKITNIRMKDQGCMLR
                     AYRRNIVESIVASGETSTFIPALAYSFAASPAEVEVEHAARQAGESKYRLYDLVRLNF
                     DLMTGFSVVPLQVFTVFGMIVSVLATIFVIYLFIRRLMVGPEAEGLFTLFAILYFLVG
                     VAIMGLGIIGEYIGRIYKEVRRRPRFVIREVYEDTND"
     gene            2543..3448
                     /locus_tag="VFK23_00435"
     CDS             2543..3448
                     /locus_tag="VFK23_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018998868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HET7317578.1"
                     /translation="MTKPAILVFAYHDVGYECLDALIQNDEYVLAVITHKDDPDEEIW
                     FRSVAGLAKKYGIPVHTPETVNTPDWIARIRSWGPDLIFSFYYRNMISEEILGIPRLG
                     AFNMHGSLLPKYRGRAPINWAILQGEKETGVTLHHMVKRADAGDIVDQERVPIGREDT
                     ARDVFGKCAKAARLVLERRLDELTAGTAPRRAQDESQATTFGGRRPEDGRIDWTTDAE
                     TIYNLVRAVTNPYPGAFTFSGGKKLFIWWAKPVSGQGGAPGEVLSTDPLVVAAGSGSV
                     EIVNWEWEDEDEDRGLAVGEVLGSS"
     gene            3609..>4296
                     /locus_tag="VFK23_00440"
     CDS             3609..>4296
                     /locus_tag="VFK23_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016209549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HET7317579.1"
                     /translation="MSLKILILGVNGFIGNSLTEHILKNKDWEVYGMDMAADKLDQCL
                     GHERFHYVEGDITINREYIEYHVKKCDVVIPLVAIATPATYVTDPIRVFELDFEANLD
                     IVRKCVRYKKRVLFPSTSEVYGMCADGEFDEETSNLVLGPISKHRWIYSCSKQLMDRV
                     IHGYGMKNGLKYTLFRPFNWIGPKLDNILEPKEGSSRVLTQFVGNILRGKNIQLVDGG
                     AQRRSFTYIDD"
BASE COUNT          920 a         1323 c         1262 g          791 t
ORIGIN      
        1 aggaaggaat gcgccggatc ggccacttcg agttcgtctg ggccaatctc gtcccgatcg
       61 ccgtgcaggt cgccggcaac gtcttcatcc tgggcggcct gacgtgctac gtcgtgagcg
      121 tcgcggtctg gctcctggtc ctgccccgcg ttgaagtaag ctacgcctac ccgctgctct
      181 ccgtgggata cattgtgaac gccgtggcgg ggtactacct gttccaggag aacctctcgc
      241 tcacgcggat cacggggatc ctgatcatct gcgtgggcgt gtatttcgtg acaaagagct
      301 gatgcgaatg cggaatgcgg aataaacgag tcattcccga atgcttttat cgggaatatg
      361 gttcttcgga cagtaatttc ataaaaccgt atctccggta gaatcgttcg gggatgacag
      421 gagtaaaaaa gataccatgt cgaacaatcc aaacgaagat ttcctcccct tctcccggcc
      481 gtccatcagc caggaggcta ttgacgaagt ggttgcatgc attaaatcgg gctggatcac
      541 cacaggcccc cgggtcaaac ggttcgaaga agacctgaag gcttatctcg gcgcaccgca
      601 cgtcctggcc ctcacttcgg cgacggccgg ccttcacctg gtgcttgtcg ccctgggcct
      661 caagcccggc gacgaagtca taaccacgcc catgacgttc gccgccacgc tgaacacgat
      721 cgtgcttgcg ggcgggaaac cggtccttgt cgatgtcgag cccggaacct acaacatgga
      781 cgtgacgaag gttgaaaagg cggtgacgcc ccgcacccgc gccatcatgc ccgtgcactt
      841 cgccggcctt cccgtggacc tcgacccgct ctacgacatc gcgaagaaac acaatcttcg
      901 cgtcatcgag gacgcggccc atgccatcgg cacggagtat aaaggtctgc ccatcggcgg
      961 gttcggcgat atccaggtct tcagcttcca cccgaacaag aacatgacga ccggcgaagg
     1021 cggatgcgtg gccacgcggg acgaacgact cacctccaac gttgccctgc tccgcttcca
     1081 cggcatggac cgcgaggcgt gggaccggtt cggtaaaaaa ggcaaccagc attacgagat
     1141 catagcgccg ggatataaat acaacatgac ggacatccag gcggccctgg gtctgcacca
     1201 gctcaaggcg ctggacgggt tcatcaggcg gaggacggag ctcgccgcgc ggtatcagcg
     1261 gctgctccag gactggcccc agtgggccct tcccgcgacg ccgccgtaca aacaccggca
     1321 tgcctggcac ctctacacgc cgctcatcaa tcccgaggcc gccggcatgg accgcgatgc
     1381 gttcatgcag ggcatgaagg agcgcaatat cgggacgggc ctgcattatc acgccgtgca
     1441 tctctaccgg tactaccgga agcatttcgg cttcaagcac ggcgatttcc cccatgccga
     1501 gacgatctcc gaccgcatcg tgagtctgcc gctcttcccc gcgatgaccg acgcggacca
     1561 ggacagggtc gttgcggcga tggaagacct gttcgggagg aaatgatcat ggccaagccc
     1621 tccataagcg tcgtcgttcc tgtttacaac gaacaggaaa atctcgaggc actcttcacc
     1681 cgcctgacga cggtcatgga caagacgggc aagccctacg agatcctctt tacgaacgac
     1741 ggaagccggg accgctccgg ggagatgctc agggatttcc acaaacgccg cccgaagcag
     1801 gtgcgcgtca tcgacttcaa cgggaacttc ggccagcata tggccatcat ggccgctttc
     1861 gagcgggcac gcggagaggt gatcgtgacc ctggacgctg acctccagaa ccctcccgag
     1921 gagatcccca aactgctcga agcggtggaa agcggccacg atgtggtcgg cggctaccgc
     1981 agaaaccggc aggacacgtt cttccggaaa tacgcctcaa aggtcatcaa cggcatccgg
     2041 gcgaagatca ccaacatccg catgaaggac cagggctgca tgctcagggc ctaccggagg
     2101 aacatcgtgg aaagcatcgt cgcgagcggc gagacctcga ccttcatacc tgccctggcc
     2161 tacagctttg cggccagtcc ggcggaggtg gaggtcgagc atgcggcgcg ccaggccggg
     2221 gaatccaagt accggctgta cgacctcgtc cgcctgaact tcgacctcat gaccggattc
     2281 tccgtggtgc cgctccaggt gttcacggtc ttcggcatga tcgtatcggt cctcgcgacg
     2341 atcttcgtga tttacctgtt catccggcgg ctcatggtcg gccccgaggc ggaagggtta
     2401 ttcaccctgt ttgcgatcct ctattttctc gtgggggtcg ccatcatggg tctgggcatc
     2461 atcggcgaat atatcggccg tatctataag gaagtgagga ggagaccccg gttcgtgatc
     2521 cgggaggtct acgaagacac caatgactaa acctgctatt ctcgtttttg cgtaccatga
     2581 cgtgggctac gagtgcctgg acgccctcat ccagaacgac gagtatgtcc tcgccgtcat
     2641 cacccacaag gacgatcccg acgaagagat ctggttccgg tccgtggccg gcctcgcgaa
     2701 gaagtacggc atccccgtcc acacgcccga gaccgtgaac acgccggact ggatcgcacg
     2761 gatacgctcc tgggggcccg acctgatctt ttccttctac taccggaaca tgatcagcga
     2821 ggagatcctc ggcatcccgc gtctgggcgc gttcaacatg cacggctctc tcctccccaa
     2881 ataccggggc cgcgcgccga tcaactgggc gatactgcag ggggaaaaag agaccggggt
     2941 gaccctccat cacatggtga agcgcgccga cgccggcgac atcgtggacc aggaacgcgt
     3001 tcctataggc cgcgaagaca cggcccgcga tgtgttcgga aaatgcgcga aggccgcccg
     3061 ccttgtgctc gaacgaaggc tcgacgagtt gacggcgggc acggcccccc ggcgcgctca
     3121 ggacgagtcg caggcgacca ccttcggcgg ccgcaggccg gaagacggcc gcatcgattg
     3181 gacgacggac gcagagacga tatataatct cgtccgcgcc gtgaccaatc cctatcccgg
     3241 agcgttcacg ttctcaggcg gaaagaaact cttcatctgg tgggcgaagc ccgtctccgg
     3301 tcagggcggc gcgccgggcg aagtgctctc gacggacccg ctcgtcgtgg ccgcggggag
     3361 cggcagcgtt gagatcgtga attgggagtg ggaagatgag gacgaggacc ggggacttgc
     3421 cgtcggagag gtgctgggaa gttcctaggt tattctcgtc cgtcataccc gccccccctt
     3481 aattcatgct ggggcaggct ccagcgggaa tccagcgtct ctgattgctc ctgatgtcga
     3541 acgtctttgg attcccgatt aaggcattcg ggaatgacgt ccaataaatc aaaaaaacgg
     3601 agaacaccat gagtcttaag atcctgatcc tcggcgtgaa cggctttatc ggaaacagcc
     3661 tgaccgaaca catcctgaag aacaaggact gggaagtcta cggcatggac atggccgcag
     3721 acaagctcga ccagtgcctg ggccacgagc gctttcatta cgtcgagggc gacattacca
     3781 tcaacaggga atacatcgaa tatcacgtca agaaatgcga tgtggtgatt cccctcgtgg
     3841 ccatcgccac gccggcaacc tacgtgacgg acccgatccg cgtgttcgag ctcgacttcg
     3901 aggcgaacct cgacatcgtc agaaagtgcg tacgctacaa gaagcgggtc ctcttccctt
     3961 ccacgtcgga agtgtacggc atgtgcgcgg acggggaatt cgacgaggaa acctcgaatc
     4021 tcgtcctggg tccgatcagc aagcatcgtt ggatctattc ctgttccaag cagctcatgg
     4081 accgggtcat ccacgggtac ggtatgaaaa acgggctcaa gtataccctc ttccgcccct
     4141 tcaactggat cggtccaaag ctcgacaaca tcctggagcc caaggaaggc agttcccgcg
     4201 tgctcaccca gttcgtcgga aacatccttc gcggaaagaa catccagctc gtcgatggcg
     4261 gcgcgcagcg caggagcttc acctacatcg acgacg
//
LOCUS       DASSLD010000027         2387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1007864, whole genome shotgun sequence.
ACCESSION   DASSLD010000027 DASSLD010000000
VERSION     DASSLD010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007864"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VFK23_00445"
     CDS             <1..382
                     /locus_tag="VFK23_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET7317580.1"
                     /translation="GRPSARQREIHALVLAAQTAAIKSVRPGMSCKTVDDAARDIIKQ
                     AGHGRHFGHGTGHGIGLMVHEGPSLSPLTRDRVEQDMVFTIEPGVYIPDWGGVRIEDM
                     VLVTENGAKVLTKLPRELESLKNA"
     gene            451..1017
                     /gene="efp"
                     /locus_tag="VFK23_00450"
     CDS             451..1017
                     /gene="efp"
                     /locus_tag="VFK23_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET7317581.1"
                     /translation="MPVSTAEFRNGLKIEIDGEPYMIVEFQLVKPGKGGAFVRTKIKS
                     LKSGNVIDRTYRSGEKVDVPELEEKTMQYLYAADKDRVFMDTSSYEQISLNERQLGDS
                     INYLKESMEIKVLYHKGQPINIEIPMFVELAIAKTDPGVRGDTASGGSKPATLETGAV
                     VKIPFYLNEGDVIKIDTRTGTFIERVKK"
     gene            1061..1531
                     /gene="accB"
                     /locus_tag="VFK23_00455"
     CDS             1061..1531
                     /gene="accB"
                     /locus_tag="VFK23_00455"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET7317582.1"
                     /translation="MNLKELKELIEMLKNSDISELEIERSGVKVRLRKGGDVTFHPAM
                     PRMEYPPAAIVAPAIPELQTTLAEKQPEPAKINQTKVTSPIVGTFYRSSSPDKPPYAE
                     VGDVVKRGQVLCIIEAMKLMNEIESETAGKIVQILVENGNPAEYGQPLFVIEPA"
     gene            1578..>2387
                     /locus_tag="VFK23_00460"
     CDS             1578..>2387
                     /locus_tag="VFK23_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET7317583.1"
                     /translation="MFKKILIANRGEIALRIIRACKEMGIKTVAVHSTADAESLHVRF
                     ADESVCIGPPKSADSYLNVPSIISAAEITDAEAIHPGYGFLAENSSFAEVCNSTGIRF
                     IGPSPESIKLMGDKARARETAQKAGVPVLPGSNGVVTEENSAIEIARELGFPVIVKAA
                     AGGGGKGMRVVMDETDFANAFVMAQAEALAAFSNADVYIEKYILQPRHIEMQIMADDK
                     GDTVYLGERECSIQRRHQKLIEEAPSVIVDEGLRRQMGEMAVAVAKSVKYRN"
BASE COUNT          645 a          582 c          700 g          460 t
ORIGIN      
        1 tgggcggcca tcggcaaggc aacgtgagat tcacgccctg gttctagctg cacagacagc
       61 ggcaataaag agcgtgcgtc ccggcatgag ctgcaaaaca gtcgatgatg ccgctcgaga
      121 cattataaag caagcaggtc atgggaggca cttcggacac ggcaccggtc atggcatcgg
      181 cctcatggtc catgaagggc cgtccctttc gccgcttacc cgcgatcgtg tggaacagga
      241 tatggtattt accattgagc cgggtgtcta tatccccgac tggggcggtg tccggattga
      301 agatatggtg ctcgtaacgg agaacggcgc gaaggtgctg actaaactgc cgagagaatt
      361 ggagagtttg aagaatgcgt agcgtttcac gatctaaaga gtaaaaaata tcatcacata
      421 gtcgaatgac gaaagggagg tagtaagaaa gtgccggtat caacagcgga gttcagaaac
      481 ggattaaaga tagagattga cggagagccc tatatgattg tggagtttca gctcgtgaag
      541 cccggcaagg gcggggcgtt cgtgagaacc aagattaaga gtctcaagtc ggggaacgtg
      601 attgaccgga cataccggtc aggtgaaaaa gtcgatgtgc ccgagctgga agaaaaaacc
      661 atgcaatatc tctatgcggc agacaaagac cgcgtgttca tggataccag cagctacgag
      721 caaatatccc tgaatgagcg gcaactcggc gacagtatca attacctcaa agagagcatg
      781 gagatcaagg tcctctacca caagggacaa cctatcaata tcgaaatccc catgtttgtt
      841 gagctcgcta tcgccaaaac cgatccgggc gtgcggggtg acacggcatc gggaggctcc
      901 aagccggcaa ccctggagac cggtgccgtc gttaagatac cgttctacct gaatgaaggg
      961 gatgtcatca agatcgacac gcgtacgggg accttcatcg aacgggtcaa aaagtgagtc
     1021 tcgatcacta tcaatactgc gacgcggaaa aggagtaacc atgaacctga aagagctcaa
     1081 ggaactgata gagatgctga agaatagcga catctcggaa ctggagatcg aacgttccgg
     1141 cgtgaaagtg aggctcagga agggtgggga tgtcacgttt catcccgcca tgccgcgcat
     1201 ggagtatcct ccagcggcta tcgttgctcc cgccatccca gaactacaga ccaccctggc
     1261 tgaaaaacag cctgagcctg caaagatcaa ccagacgaaa gtgacctctc ctatcgttgg
     1321 aacattctac cggtctagtt caccagacaa gccgccctat gccgaggtcg gtgatgtcgt
     1381 gaaaaggggg caggtgctct gtatcataga ggcgatgaag ctaatgaacg agattgaatc
     1441 cgagacagca gggaaaatcg tccagatcct tgtcgagaac ggaaatcccg cggaatacgg
     1501 gcagcctctc ttcgtgatcg aacctgcgta atgccgcaat ttgagacttg aactttgagc
     1561 tccaatgagg tgcctgagtg tttaaaaaaa tattgatcgc caaccgcggc gagatcgcgc
     1621 tacgcatcat acgagcgtgt aaggagatgg gcatcaagac cgtcgccgtt cattctactg
     1681 cggatgccga gtcactccat gtgcgttttg ccgatgagag cgtgtgcatc ggaccgccga
     1741 aaagcgctga cagttatctc aacgtaccga gcatcatcag tgccgccgaa atcacagatg
     1801 ccgaagccat ccatccgggc tacggcttcc tggcggaaaa ttccagcttt gccgaggtat
     1861 gtaactccac cggcatacgc ttcatcgggc cgagtccgga gagcatcaaa ctcatggggg
     1921 acaaggccag ggccagggaa acggcccaga aggcaggtgt tcccgtgctg ccgggcagca
     1981 acggcgtggt tacggaagag aacagtgcca tcgagatcgc gcgggagctt ggattcccgg
     2041 ttatcgtaaa agcagcggca ggcggtggtg gtaaaggcat gcgcgtcgtg atggacgaaa
     2101 cagacttcgc caatgccttt gtcatggcgc aggccgaagc gcttgccgcc ttcagcaatg
     2161 ctgatgtgta tatagaaaaa tatattctcc aaccgcgcca cattgagatg cagataatgg
     2221 cggacgacaa gggcgacacc gtctatctcg gtgaacggga atgttcgata cagcggagac
     2281 atcagaaact gatcgaggag gccccttcgg tcattgtcga tgagggtctg cggaggcaga
     2341 tgggagaaat ggcagtggca gtggccaaaa gcgtgaaata ccggaat
//
LOCUS       DASSLD010000028         3762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_909255,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000028 DASSLD010000000
VERSION     DASSLD010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909255"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFK23_00465"
     CDS             <1..288
                     /locus_tag="VFK23_00465"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HET7317584.1"
                     /translation="GYREIMLNEDCVIGNKGETCRGHEFHYSEIEAGTLTQDAGGRKE
                     IYRISDKSGGHVRDEGFRLMNTLASYVHVHFGSNPNIAQRFATFVTGERIA"
     gene            285..1304
                     /locus_tag="VFK23_00470"
     CDS             285..1304
                     /locus_tag="VFK23_00470"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: protein motif:HMM:NF014612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HET7317585.1"
                     /translation="MKESILLLGHGSPKKDANNLDRVGKMLHGMLHAGCNEDCVKVAY
                     LQFVEPGIPEAIRECVEQGAKKIILHPFFLSAGMHVTKDIPGMIEEARGRYPEVKFVY
                     TEPLGIHEKLAHIVMERISAADDLAPAAIEKRSFDILSAEADLSSIPADRLPIVQRVI
                     HATGDFEFKNTLTFHPDAVAYGLKAIKAGKDILTDVEMVKTGISRRWLSPWGGKVICG
                     IQNTGAQGAAEQAEEGKTTTRAERGIEEALRQNRNIGIIAIGNAPTALLKTIDLLTNS
                     ELRTRNSELLVVGVPVGFVKAVESKALLATQDFPFITALGRKGGTPAAVAIVNALLKM
                     AGENK"
     gene            1460..2134
                     /locus_tag="VFK23_00475"
     CDS             1460..2134
                     /locus_tag="VFK23_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HET7317586.1"
                     /translation="MHISEGILPFNWAALWFGVALPFVGFGLYRLKKMSAADLSFKPL
                     VGLMAAVVFIISCMPIPVPSAGTCSHPCGTGISGILLGPAISVLITAAALLIQALFLA
                     HGGLSTWGADIVSMGVVGSFAGYFTFKALRAVKINMAVAGFMAGLLADWATYLTTSVE
                     LASGIRGESPFLPLFWKIMIAFIPTQLPLGILEGAMTSGMVVLLYKKRPDLLVKMGVV
                     KAGEAQ"
     gene            2137..2415
                     /locus_tag="VFK23_00480"
     CDS             2137..2415
                     /locus_tag="VFK23_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter"
                     /protein_id="HET7317587.1"
                     /translation="MRNSEFGIRNCLVAFLLIALCAASAAGAEKWTGVDESVVQKIAK
                     EQGREAREPFINTDRGDLLLFVFLTAGAIGGFAAGYYWRKLTEPGRRE"
     gene            2422..3174
                     /gene="cbiQ"
                     /locus_tag="VFK23_00485"
     CDS             2422..3174
                     /gene="cbiQ"
                     /locus_tag="VFK23_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HET7317588.1"
                     /translation="MEMFSKQVKKDHVLSRVDARIKILVALVLLAMVLSYRGFVFPVV
                     TAVLCLLFCMTMRVPMRVFVLRFAEPLFIAAVVLVLKFFFSGKDTLFSLHVLGFDITG
                     HRDGLSEGLMIGSRIIGAVSIVALAGFSTPFTEFMAGLSWFRVPKGFIEILMFAYRYI
                     FVLLDDANVIYNAQKNRLGYSTLRRGLSSFGVLAGSLTLKAFDHSHNTTVAMVQRGYD
                     GNMPLLKHKSFKPFEVAASILFVAGMGLLWKM"
     gene            3195..>3762
                     /locus_tag="VFK23_00490"
     CDS             3195..>3762
                     /locus_tag="VFK23_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7317589.1"
                     /translation="MQLSVDIRSFQYPDGTKALSDISLEVHQGEFVGLLGSNGSGKTT
                     LLKAMDGLMKDFDGRVRLNDADVRKLSPKEIYGKVGLVFQNPDDQLFAPTVFEDVAFG
                     PLNMGLPQDEVARRVHRALNAVEMDGFAGKSIHNLSFGQKKRICIGGLLAMGHELLLL
                     DEPTAGLDPMGEYKMMALLTKLNLEQKVT"
BASE COUNT          797 a         1028 c         1135 g          802 t
ORIGIN      
        1 ggctaccgcg agattatgct gaacgaggat tgcgtcattg ggaacaaagg cgagacgtgc
       61 cggggacatg agttccatta ttcggaaatc gaagccggaa cgctgacgca ggacgccggg
      121 ggccggaaag aaatctatcg gatcagcgat aaatcggggg ggcatgtccg ggacgagggg
      181 ttcaggttaa tgaacaccct cgcgagctac gtgcacgtac acttcggcag caacccgaat
      241 atcgcgcaac ggttcgcaac gtttgtgacg ggagaacgca tcgcatgaag gaaagcattc
      301 tgcttctcgg acacggaagc ccgaaaaaag acgcaaacaa cctggatcgc gtgggaaaga
      361 tgctgcacgg catgctgcat gccgggtgca atgaggactg cgtcaaggtg gcctatctcc
      421 agttcgtcga gccgggcatc ccggaggcca tcagggaatg cgtggagcag ggtgcgaaaa
      481 agatcattct ccatcctttt ttcctgagcg ccggaatgca cgtgaccaag gacattcccg
      541 ggatgatcga ggaggcgcgg ggacgctatc ctgaagtgaa gtttgtctat accgagccgc
      601 tcggcatcca tgaaaaactg gcccacatcg tgatggagcg gatcagcgca gcagacgacc
      661 tcgcgccggc ggccatagaa aaaaggagct tcgacatcct ttccgccgag gcggacctga
      721 gcagcatccc cgccgaccgc ctgccgatcg tgcagcgcgt catccatgcc acgggggatt
      781 tcgagttcaa gaacacgctg acgtttcatc ccgacgcggt cgcttatggg ctcaaagcga
      841 tcaaggcagg gaaagacata ttgaccgacg tcgagatggt gaaaacaggg atcagcaggc
      901 gatggctttc accctgggga ggaaaggtca tctgcggcat tcaaaatacc ggggcgcagg
      961 gggctgcgga gcaggcagag gaaggaaaga ccacgacacg cgcggagcgg gggatcgaag
     1021 aggccttgcg gcagaaccgc aacatcggta tcattgcaat cggcaatgcg cccacggcct
     1081 tgctgaagac catcgatctt ctcacaaact ccgaactccg aactcgtaac tccgagctac
     1141 ttgtggtcgg cgttccggtc gggttcgtga aggccgtgga atccaaggcc ctcctcgcaa
     1201 cacaggattt cccctttatc acagcgctgg gaagaaaggg cggcacgccg gcggcggtgg
     1261 cgatcgtgaa cgcgctgctg aagatggcgg gggagaataa atgaaagttc ggagtttccc
     1321 cgataaaatc attcgaggac aggcttcgtt cggagttcgg agtgtaatgc ggagacgaaa
     1381 agaccgatat cttgcgacgg ttttcttttt actttgtact ctgaactttg ttctctgtac
     1441 tgcttccgac gtttacgcca tgcacatctc cgagggtata ttgccgttca actgggcggc
     1501 cctctggttc ggcgttgcgc tgccgtttgt cggcttcggg ttataccggc tgaagaagat
     1561 gtccgcggcc gacctgtcgt tcaagccgct cgtgggcctc atggccgcag tcgtctttat
     1621 catctcctgc atgccgatcc cggttccgtc ggcgggcacg tgttcgcacc cctgcggaac
     1681 ggggatttcg gggatacttc tcggaccggc catcagcgta ctgatcaccg ccgcggccct
     1741 tctgatccag gcgctttttc tcgcccatgg gggcttaagc acgtggggcg cggacatcgt
     1801 ctccatgggt gtggtcggtt cctttgccgg atatttcacc ttcaaggctt tgcgcgcggt
     1861 aaaaataaat atggcggtcg ccgggttcat ggcaggtctt cttgccgact gggccacgta
     1921 tctcactacg tcggtcgaac ttgcctcggg catacggggg gaatcgccat ttcttcccct
     1981 cttctggaaa atcatgatag ccttcatccc gacgcagctc ccgctgggga ttctggaagg
     2041 agcgatgaca tcgggcatgg tcgtcctcct gtataaaaaa aggccggatc tgctcgttaa
     2101 aatgggtgtg gtgaaagcgg gggaggcgca gtgacaatgc ggaattcgga gttcggaatt
     2161 cggaattgtc tcgttgcttt tctgctcatc gcactttgtg cggcatccgc cgccggcgcg
     2221 gaaaaatgga ccggcgtcga tgaatccgtg gtccagaaaa tcgcgaagga acagggcagg
     2281 gaggcgcggg aaccgttcat caacaccgac cggggagatc tcctgctttt cgtttttttg
     2341 accgccggag caatcggcgg ctttgccgcg ggatactact ggagaaaact gaccgaaccg
     2401 ggacggaggg aatagcggac gatggaaatg ttctccaaac aggtgaaaaa agaccatgtc
     2461 ctctcgcggg tcgatgccag gataaaaata ctcgttgccc tggtccttct ggccatggtc
     2521 ctgagctatc gcggattcgt ctttcccgtt gtcacggcag tactctgcct gcttttctgc
     2581 atgaccatgc gggtgcccat gcgggttttt gttctgagat tcgccgagcc cctgttcatt
     2641 gccgccgtgg tgctcgttct caaattcttc ttctccggga aggatacgct cttttccctg
     2701 catgttctcg gctttgacat taccggacac cgggacgggc ttagcgaagg attgatgata
     2761 ggctccagaa tcatcggcgc ggtgtccatc gtggccctcg ccggattttc cacgcccttt
     2821 accgagttca tggcgggcct ctcctggttc agggtcccga agggttttat cgaaatcctg
     2881 atgttcgcct accggtatat cttcgtgctg ctcgatgacg caaacgtgat ttacaacgca
     2941 cagaaaaacc gtctcgggta ttcaaccctg cggcgcgggc taagctcctt cggcgtgctc
     3001 gccggttcgc tcacgctcaa ggccttcgac cacagccata acacgaccgt cgccatggtc
     3061 cagcggggct acgacgggaa catgccgctc ctgaagcaca agtcgttcaa accattcgag
     3121 gttgccgcgt ccattctttt cgtggctggc atgggtctgc tatggaagat gtgaaggaag
     3181 ggccgtcggc acgcgtgcag ctttccgtcg atattcgttc ctttcaatac cccgacggga
     3241 caaaagccct gtccgacata tcccttgaag ttcaccaggg tgagttcgtc ggccttctgg
     3301 ggtccaatgg ttcagggaaa acgacgctcc tcaaggcgat ggacggattg atgaaggatt
     3361 tcgacggacg agtccggctc aatgacgcgg atgtcaggaa actatcgccc aaggagatct
     3421 acgggaaggt cggcctcgtg ttccagaacc ccgacgacca gctatttgca ccgaccgtgt
     3481 ttgaggatgt cgccttcggg ccgttgaaca tggggcttcc ccaggacgag gtcgcacgcc
     3541 gcgtacaccg cgccctgaat gccgtggaga tggacggctt cgcggggaag tccattcaca
     3601 acctgagctt cggccagaag aaaaggatct gcataggcgg tctccttgcg atgggacacg
     3661 aactgctgct gcttgacgag ccgacggcgg gcctggaccc catgggagaa tacaagatga
     3721 tggccctctt gacgaagctg aacctcgaac agaaggtgac gg
//
LOCUS       DASSLD010000029         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1398501, whole genome shotgun sequence.
ACCESSION   DASSLD010000029 DASSLD010000000
VERSION     DASSLD010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398501"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFK23_00495"
     CDS             complement(<1..407)
                     /locus_tag="VFK23_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HET7317590.1"
                     /translation="MASTTFLDKTLLAIADAAEQSLFSEQFANRRGLLQALDIRFQLV
                     TFLFLLVLVSLLHTPQSLWLIYGAGLAIAALSRVPLWFFVKRVWLFVPLFSAAVVLPA
                     VLNIITPGDPLLVLVKLPQSYSCGPYSLPAEITV"
     gene            complement(563..910)
                     /locus_tag="VFK23_00500"
     CDS             complement(563..910)
                     /locus_tag="VFK23_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HET7317591.1"
                     /translation="MTTTKKLWIGIGILVLLSPLGLVLPALFGSQGAWGEWGIHQIEK
                     LVGYVPEGMKRLAGRWRAPLPEYAVPGQGQGPAGSGFGYVLSGIIGIAATAGLVYLLA
                     KLLVRKDGSNERK"
     gene            complement(1004..1672)
                     /gene="cbiM"
                     /locus_tag="VFK23_00505"
     CDS             complement(1004..1672)
                     /gene="cbiM"
                     /locus_tag="VFK23_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HET7317592.1"
                     /translation="MHIPDGYLGPATYVTLYGVTIPFWAAASWKLNRTLKARQAPYLA
                     IGAAFTFVIMMFNIPIIGGTTGHATGATLIAILLGPWAAVIAVSAALIIQALLFGDGG
                     VTAIGANCFNMAVAGVFVGYGIYRGVAAGAGPASRRRVVAGAVAAYVSLNASALLAAV
                     QLGLQPLLERSPAGQPLYAPFPLHVAVPAMVLPHLAVFGFAEAAVTALVIKYFQKNDP
                     GMMG"
     gene            complement(1819..2577)
                     /locus_tag="VFK23_00510"
     CDS             complement(1819..2577)
                     /locus_tag="VFK23_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET7317593.1"
                     /translation="MAYFVRSILLIAMLLFPVAAMAGHQLEVEDTATQGKGNFLFELT
                     GDYLKSNEFKTTKWTGIITAGSGEHTEVTLEVPYLLQHQNLVDQNVSGKGDVRLKMKG
                     QIFENEVKQSMAYLVFVDLPTGDAAKGLGTDNVVWGVKLVDSQECRNCIFHVNLGYEV
                     FGKALRKGHFEEDYALTFGFAAGHRFTNSFRVLTEIAGEIRKEPDRNLKPFTLLAGIV
                     YDIAKSWYVDLGARAGLNKDAEDYAVLAGTGWRF"
BASE COUNT          599 a          809 c          747 g          559 t
ORIGIN      
        1 actgtgatct ccgcaggcag cgaataaggg ccgcatgaat aggactgggg gagtttcacg
       61 agcaccagga ggggatcccc cggcgtaatg atattcagga ccgcgggaag cacgacggcc
      121 gccgagaata gcggcacgaa cagccagaca cgctttacga aaaaccacag cggcacccgc
      181 gacaacgccg cgatcgccag gccggcgccg tagatcagcc agagggactg cggcgtgtga
      241 agcaggctga cgaggaccag gaggaacaga aaggtcacga gctggaacct gatgtcgagt
      301 gcctgcaaga gtccgcgtcg gttcgcgaac tgctccgaga acagcgactg ttcagccgcg
      361 tccgctatcg ccagaagtgt cttgtctaag aaagtcgtac tcgccaagtt atgcagacct
      421 cattttttat gccttcagca tccattctgt cattccgggc ttgacccgga atccattggc
      481 tttagaagaa taaaaggatt cctgccttcg caggaaaata gcaaaaaaga ggctttgttt
      541 tcgccaagtt cctatcgacc gatcattttc gttcgttgga accgtctttt cgcaccagca
      601 gcttcgcgag aagataaaca agacccgccg ttgccgctat cccgatgatt ccggaaagca
      661 catatccgaa accgcttccg gcgggaccct gcccctgccc gggaacggca tactcgggaa
      721 gcggcgccct ccatctgccc gcaagtcgct tcatgccctc gggaacatag cccacgagct
      781 tttcgatctg atgaataccc cactcgcccc acgcgccctg cgagccgaaa agcgccggta
      841 ggaccaggcc cagaggcgat agcagcacca ggatgccgat gcctatccaa agcttttttg
      901 tggtggtcat gctgtcacac gttgcgaatt gacgcgttac cgttcgcgtg ttcaacacgt
      961 aacccgcaac tttcattccg caatccgcat tccgaattcg actttacccc atcatccccg
     1021 gatcattctt ctgaaagtac ttgatgacga gggccgttac cgcggcctct gcgaagccga
     1081 acacggccag gtgcgggagc accatcgcgg gcacagcgac gtggagggga aagggcgcgt
     1141 agaggggttg accggctggc gaccgttcga gcagcggctg aaggcccaat tgcactgctg
     1201 cgagcagggc cgacgcgttc agactcacgt acgcggcgac cgctccggcg acaacccgtc
     1261 tgcggcttgc ggggcctgct cccgcggcga ccccgcggta tatgccgtaa ccgacgaaca
     1321 ctccggcaac ggccatgttg aagcaattcg ctccgattgc tgtgacgccg ccgtctccga
     1381 agagaagcgc ctggatgatg agcgcggccg acacggcgat caccgccgcc cagggaccga
     1441 gcaggatggc aatcagcgtg gcccccgtcg catggcccgt cgtgccgccg atgatcggga
     1501 tattgaacat catgatgacg aaggtaaagg ccgcgccgat ggcgagataa ggcgcctgcc
     1561 gtgctttgag cgtcctgttc agcttccagg acgccgcggc ccaaaaaggg atcgtgaccc
     1621 cgtagagggt cacgtaggtg gcggggccga gatatccgtc agggatgtgc atgattcaaa
     1681 aaccgcgata cactgcgccg ccagtggccg cagccagaaa gtttgtcatc cccgaatgat
     1741 tctatcgggg atccggtgtt ttctcaaacc ttaaaagacg tattcccgat aaagccgttc
     1801 gtgaatgacg aataggtgtc aaaaccgcca gcccgtcccg gccagcaccg cataatcctc
     1861 ggcgtccttg ttcaacccag cccgcgcgcc gagatcgacg taccatgatt tcgcgatatc
     1921 atagacgatc cccgcaagca atgtgaacgg tttcaagttc cggtccggtt ctttcctgat
     1981 ttctcccgcg atctctgtca ggacacggaa cgagttcgta aaccggtgtc cggccgcaaa
     2041 accgaaggtg agggcataat cttcttcaaa atggcccttt ctcagggcct tgccgaacac
     2101 ctcgtacccc aggttcacgt gaaagatgca gttcctgcat tcctgcgaat cgacgagctt
     2161 gacgccccag accacgttgt ccgtaccgag ccctttggcg gcatcgcccg taggcagatc
     2221 gacgaagacc aggtaggcca tggactgttt gacctcgttt tcgaaaatct ggcccttcat
     2281 ttttaaccgc acatccccct tgccgctgac gttctgatct accaggtttt gatgctgcag
     2341 gagatagggc acttcgaggg taacttccgt gtgctctccg gagccggcag tgatgatccc
     2401 ggtccatttc gtggttttga actcattgct tttcaggtaa tctcctgtaa gttcgaacag
     2461 gaagtttccc ttgccctgtg tagccgtgtc ctcgacttcg agttgatggc cggccatcgc
     2521 ggcaaccgga aacagaagca tcgcgataag aagtatggaa cgcacaaaat aagccatttc
     2581 acggtctccc tggttcaata cagataggaa aggttataca gtaatttatt tttcgcgtca
     2641 accgtcattt tgcgcgctcg cgggattatc gccttgacac cgttttcctc gtcatgctat
     2701 cgttgtcctg cccg
//
LOCUS       DASSLD010000030         2171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_745644,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000030 DASSLD010000000
VERSION     DASSLD010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745644"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFK23_00515"
     CDS             <1..314
                     /locus_tag="VFK23_00515"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HET7317594.1"
                     /translation="PRDTVGMIGDLFLDPSTYLVASDVLAGIKAYEKVNATSLVIGEY
                     ESLKDAAVDPYVALRDAYVQYRLNKVKSGRGKPVTSSTEGASSPTIREGPPSIETKDE
                     K"
     gene            complement(376..573)
                     /locus_tag="VFK23_00520"
     CDS             complement(376..573)
                     /locus_tag="VFK23_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7317595.1"
                     /translation="MMLEGKQSILKTRDLALLLDLSPDVVNDMARRGYLKGFKSGNQW
                     RFRRRDVERYMERIKKMTPPG"
     gene            753..1397
                     /locus_tag="VFK23_00525"
     CDS             753..1397
                     /locus_tag="VFK23_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317596.1"
                     /translation="MLIVDAVLLRSLLVRMANHLTSTHGLILLSGALAYLCLHFLLRK
                     PERMYLWAHEVTHLLVAKLFLRHVHGFHITSRSGGKVVIDRTNVAIDLAPYAVPLYNV
                     VALLPVTLFAAGLPLGGKIYLGAAAFLFTMHLCFSVEGFIDGQPDVRRSGRIFSLAVV
                     LLLLLLWTPILAAPGSQGGFAGLSGFYREWFASGMEATLGLPARVRSLLSHRFL"
     gene            1408..>2171
                     /locus_tag="VFK23_00530"
     CDS             1408..>2171
                     /locus_tag="VFK23_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317597.1"
                     /translation="MVKTEEKRIRPGDIFLLAILLANLVCGVGLLGALRADLSKDLVA
                     SESALASSRINAYLWGKILRFTFAVEQGRKAGENTPVKGDADVQSVVADLPAEWDFHR
                     IRSGTGEWPAVFMGTPRDLPNRGHVLPLGVLVHGGARQGQRYVIGAISLADVDGYLGD
                     LRSRGILCRIVDPDGRTLLGTTGGMPVRRRSGSIFEGEAPVMGGALSREWFLQTFIPD
                     ASSRLPFLPQAILATFFLLNIATIVFLRQRFILPAEA"
BASE COUNT          407 a          630 c          641 g          493 t
ORIGIN      
        1 gcccccggga tacggttggg atgatcggtg atttgttcct ggatccgtcc acataccttg
       61 ttgcttcgga tgtcctggca ggtatcaagg catacgaaaa ggtgaatgcc acatccttgg
      121 tgatcggcga atatgagtcc ctgaaagatg cggccgtcga tccctacgtg gcgctccgcg
      181 atgcctatgt gcaatatcga ttgaacaagg tcaagtcagg gagaggcaaa ccggtgacat
      241 caagcaccga aggcgcaagc tcaccgacga taagggaagg tccgccatcg atagaaacta
      301 aggacgaaaa ataaatatcc gacgtgataa tcttggaaaa gagaagcctc ataaaccttt
      361 ggccgggcgt tcgcgtcacc ccgggggcgt cattttcttt atccgctcca tgtatctctc
      421 aacgtcccgg cgccggaatc gccactggtt tccgctcttg aaccctttca ggtacccgcg
      481 acgcgccatg tcgtttacga catcgggact gagatcgaga agtaacgcca ggtcgcgcgt
      541 cttgaggatg ctctgtttgc cctctaacat cattgggcct ccccatggcg gcataatgac
      601 agttagggcg aaaataacat aacgttatat ataagtaaac ggttgattat ttgtcgaggg
      661 aaataaatgt ttgtcttttt taatcaagaa tttatgttcc aagctgggat ttcgacgtga
      721 gagtcttcct ttattcagtg atcggactac ctgtgctgat cgtagacgcc gttcttctcc
      781 ggtcgctcct ggtgcggatg gcgaaccacc tcacaagtac ccatggactg atccttctat
      841 ccggtgcgct cgcctacctc tgccttcatt ttctgttaag gaaacccgag cggatgtatc
      901 tctgggcgca cgaggtcacg catcttctcg tggcgaaact ctttcttcgg cacgtccacg
      961 gtttccacat cacctcacgc tccggtggga aggttgtgat cgaccggacg aatgtggcga
     1021 tcgacctcgc tccgtacgcg gtgccgctct acaacgtcgt cgcgctcctt ccagtgacgt
     1081 tgttcgcggc gggattacca ctcggaggga agatctacct cggcgcggcg gcgttcctgt
     1141 tcacgatgca cctctgcttc tccgtggagg ggttcatcga cggacagccg gacgtacggc
     1201 gcagtgggag gatcttctcc cttgctgttg tcctgctcct tctgttgctg tggacgccga
     1261 tcctggcggc gccggggagc caaggggggt ttgccgggct gtccggtttt taccgcgagt
     1321 ggttcgccag cgggatggag gcgactctcg gtcttccggc gcgcgtccgc tcccttcttt
     1381 cgcaccgttt cctgtaaact atccgtcatg gtgaaaacgg aagaaaagcg catccgcccg
     1441 ggggatattt tcctgctcgc catcctcctc gcgaacctgg tgtgcggagt cggcctcctc
     1501 ggcgcgttgc gggcggatct ttcgaaagac ctggtcgcga gcgaaagcgc gctcgcttcc
     1561 tccaggatca acgcgtacct gtgggggaag atcctcaggt tcaccttcgc ggtggagcag
     1621 gggaggaagg cgggggaaaa cacgccggtc aagggggacg ccgatgtgca atcggtcgtc
     1681 gccgatctgc ccgccgaatg ggacttccat cgcatacgat ccgggacggg ggagtggccg
     1741 gccgtcttca tggggacccc ccgggatctt ccgaaccgcg ggcacgttct cccgctcggg
     1801 gtcctcgtcc acggaggagc gaggcagggg caacgatatg tcatcggggc gatttccctc
     1861 gcagacgtcg acgggtacct cggtgacctc agaagcaggg gaatcctttg ccggatcgta
     1921 gatccggatg ggcgcaccct gttaggaaca accggcggga tgccggtccg ccgccgttca
     1981 ggatccatat tcgagggaga ggcgccagtg atgggcgggg cgctctccag ggagtggttc
     2041 ctgcagacgt tcatcccgga cgcgagttcc cgtctgccgt tcctgccgca ggccatcctt
     2101 gccaccttct tcctcctcaa catcgccacc atcgttttcc tccggcaacg ctttatcctg
     2161 cccgcggagg c
//
LOCUS       DASSLD010000031         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_386738,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000031 DASSLD010000000
VERSION     DASSLD010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386738"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1853)
                     /locus_tag="VFK23_00535"
     CDS             complement(<1..1853)
                     /locus_tag="VFK23_00535"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HET7317598.1"
                     /translation="MKSAVSVSAPASGKGLNLSENCLKVLEKRYLKRNEDGTVTETAV
                     DMFTRVARTIAEADRAYGKSDAEVERTATEFYRLMTSLEFLPNSPTLMNAGRELGQLS
                     ACFVLPVGDSMDEIFESVKQAALIHKSGGGTGFSFSRIRPKNDVVKSTKGVSSGPISF
                     MTVFDAATETVKQGGTRRGANMGILQVDHPDILDFVTCKQQNDRLNNFNISVAITDPF
                     MEAVEKDDEYDLVSPRNKGVVKRLHARRVFDTVVNMAWKNGDPGIIFLDRINQYNPTP
                     HVGKVESTNPCGEQPLLPYESCNLGSINLARMIVKHGRAVDYDKLSHTVQQAVHFLDN
                     VIDVNRYPIEKISQMTRANRKIGLGVMGFADMLLEMGIPYNSDEAVKLGESVMKFITD
                     EGRKMSEELARERGAFPNFKGSIYDRPGMAPVRNATVTTIAPTGTLSIIAGCSSGIEP
                     LFALTFVRKVLDGAELLEVNPVFERIAKERGFYSDDLMKDIAEHGGCTGIPTVPEDIQ
                     RVFVTAHDITPEWHIRMQGAFQKHTDNAVSKTVNFPHSATQKDVEKVYMLAYKLGCKG
                     VTIYRDGSRDEQVLSTGATEKARKAAAAGHSVTVASASPELAEEKKPRHRPL"
BASE COUNT          495 a          635 c          630 g          535 t
ORIGIN      
        1 agcggacgat ggcgcggctt cttctcctcg gccagttcgg gtgacgccga agcgaccgtg
       61 accgaatggc cagctgccgc ggccttgcgt gccttttccg tagcgcccgt gctcagcacc
      121 tgttcgtcac gcgagccgtc gcggtagatg gtcacaccct tgcagccgag cttatacgcc
      181 agcatataca ccttctcaac atccttctgc gtagcgctat gcgggaagtt cacggtcttg
      241 gacaccgcgt tatcagtatg cttctggaac gcaccctgca tgcggatatg ccactcagga
      301 gttatgtcgt gggccgtgac gaacacgcgc tgaatgtcct cggggaccgt cgggatgccc
      361 gtgcaccccc cgtgctcggc aatatccttc ataagatcgt cggaatagaa gcctcgctcc
      421 tttgcaatcc tttcgaacac ggggttcacc tcgaggagtt cagccccgtc tagcaccttc
      481 cggacaaagg taagggcaaa gagaggttcg atgccgcttg agcagcccgc gatgatcgag
      541 agcgtgcctg tcggagcaat tgtcgttacc gtcgcattcc gcaccggcgc catgcctggt
      601 ctgtcataga tacttccttt aaagttcgga aaggccccgc gctccctggc gagttcctca
      661 ctcatcttcc ggccttcgtc ggtgatgaat ttcataaccg actccccaag tttgacggcc
      721 tcgtcggagt tgtatgggat gcccatctcg agcagcatgt ctgcgaagcc cataacgccg
      781 aggccgatct tgcggttggc gcgggtcatc tggctgatct tttcgatggg atagcgattc
      841 acatcgatca cgttgtcgag gaagtggacc gcctgctgca ccgtgtggct aagtttgtcg
      901 tagtccacgg ccctgccgtg cttcacaatc atccgggcca ggttgatcga gcccaggttg
      961 caggactcat acggcagaag cggctgctca ccgcaggggt tggtggattc caccttgccc
     1021 acatgcggcg tagggttgta ctggttgatg cggtcaagga aaataatgcc cgggtcgccg
     1081 ttcttccaag ccatgttcac aacagtatca aagacccggc gagcatgaag gcgcttgacc
     1141 acgcccttgt tccggggcga cacgagatca tactcatcgt ccttctctac cgcctccatg
     1201 aaggggtcgg ttatggcaac ggagatatta aagttgttca gccggtcgtt ctgctgcttg
     1261 caggtgacga agtccaggat gtccgggtgg tccacctgca ggatgcccat gttcgctccg
     1321 cggcgcgtgc caccctgctt caccgtctcc gtggccgcat cgaagacggt catgaacgaa
     1381 atggggcccg acgatacacc cttggtggat ttaaccacat cattctttgg acggatgcgg
     1441 gagaaagaga agcccgtacc gccgccggac ttatggatga gcgccgcctg cttcacggac
     1501 tcaaagatct cgtccatgga gtcgcccacg ggcagcacga aacaagccga gagctggccg
     1561 agctcccgtc ccgcgttcat gagcgtgggg ctgttcggca ggaactcgag ggacgtcata
     1621 aggcggtaga actcggtcgc ggtccgctcc acctcggcgt ctgacttgcc gtaagcccga
     1681 tcggcttcgg cgatggtccg cgctacgcgc gtgaacatat ctactgctgt ttcggtaacg
     1741 gtcccgtcct cgttccgttt gaggtagcgt ttttcgagca ccttgaggca gttctcggac
     1801 agattgagac ccttgccgga tgctggtgcc gaaacagaaa ccgcgctctt catgattccc
     1861 tccctggcta atgttaaaag aaattataaa ttcaaatcaa agccggggcg gcttgatgac
     1921 cgccccggca ttattttact acttgtaaga ttgactagcc gaacgattaa aacagtattt
     1981 tagtgaattt ctcatctggc cctgcctgtg atgtgtttct cttgggtgaa gcctattgag
     2041 gttttttgcg tgaagctagg agaagcaaaa accagcagga aacgatccta cagagtcaaa
     2101 actgctagga agtataacca caagatattg tattgtcaag acgattttta taccatattt
     2161 aggcaatttt catgcggctt ttataataaa ataccctttc caagagcata gaatctggat
     2221 gaaaaaaaac aaggttttaa aaaccctcgc caaatgcgga tatgatgaaa aatgaggagt
     2281 aaaggggtct agatc
//
LOCUS       DASSLD010000032         4031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_28086,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000032 DASSLD010000000
VERSION     DASSLD010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28086"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VFK23_00540"
     CDS             <1..964
                     /locus_tag="VFK23_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET7317599.1"
                     /translation="LLFMGAGAVLHATHERNMEEMGGLIRFMPWTAVLFLIGCVSISA
                     LPPFNGFVSEWLTYQAFLLSPALPSPLLNLLIPLGAALLALTSALAAACFVKAFGVTF
                     LGHQRGHHHGRIHEADWSMRIGMLLAALTCLALGVLPTVMIRWMDPLAEELVGGTIAA
                     SASGIGWMWLTPVAAERASYAAPIAFMGILSVVVVTYLLLHSRPGAIHRVPLWDCGFE
                     KITSRMQYTATSFSMPLRRIFGFLFSIKEHVRQIPPVSHPAFPQRLQYHLRVRDRFWM
                     WMYQPVVDASFWLSRRIGRMQQGRIQIYLIYSFVTILVLLIFSR"
     gene            1228..2172
                     /locus_tag="VFK23_00545"
     CDS             1228..2172
                     /locus_tag="VFK23_00545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HET7317600.1"
                     /translation="MNIHPLIIEVLQVAVLLFVAPLFAGWVKMVKCWFQGRSAPSLFQ
                     RYRDIRKLLAKDVTLAENASWIFRFTPYLVFGVSVMAGGIIPILSVDLPLSATADVIA
                     LVALFAIARFFTALAGMDIGTAFGGMGSSREMTIASLAEPAMLMAVFAVSLVSHSTSL
                     SHMTAVISQGHSLLRPSLVFALLAFVLIALAETGRVPVDNPATHLELTMIHEAMILEY
                     SGRHLALIEWANMMKLFLFTALGIAAFFPGGIAPDDDLLAVAAALPILLIKLAIVGVG
                     LVTIETGLAKMRIFRITEFLGSAFLFATLGMLSYFMLE"
     gene            2331..2996
                     /locus_tag="VFK23_00550"
     CDS             2331..2996
                     /locus_tag="VFK23_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HET7317601.1"
                     /translation="MNLHLAAQINSLLAAMILLTAFALVVQRRMNALLHLFAWQGLLL
                     AVSTAIVGYVGGQHDLYISSVLTLSMKVILLPYILHKLIVKLRIHKEVETVVNIPTTM
                     LMGIVLVIFSYHLTAPIRELSTLVTRSTLAVALATVMLGFLMMITRKHAVTQIIGFLA
                     MENGLLFAATSATYGMPLVVELGVALDLLIAAFIFGMFFFHISTTFDSLDVDQMARLK
                     EED"
     gene            3079..>4031
                     /locus_tag="VFK23_00555"
     CDS             3079..>4031
                     /locus_tag="VFK23_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HET7317602.1"
                     /translation="MELLVLLGVPLVAALALAFVGDRKLAPEVNILGSAATFIASVKL
                     ALEVYGRGSILAGNNFFFVDAFSVYLTVLTAFVSMTTAIFSRRYMRREREHGTVGHRR
                     LRFYHSMYQLFIFAMLLCLLTNNVGILWIAMELATLSTVLLVSLYRTPAAIEAAWKYF
                     ILCGVGIALALFGTVLLYFAAEKVLGEGGAALHWTNLYQVSNQLEPTILSLAFVFLMV
                     GYGTKVGLVPIHNWLPDAHSEGPTPISAVLSGLLLNIALYALVRCKVLVDGSTGTHHS
                     GDIMMGFGVISIIVASFSLLRQKDIKRLFAYSSIEHMGIAT"
BASE COUNT          695 a         1243 c         1109 g          984 t
ORIGIN      
        1 actgctcttc atgggcgccg gcgccgtgct ccacgcgacc catgaacgga acatggagga
       61 gatgggcggg ctcatccgct tcatgccctg gaccgccgtg ctcttcctca tcggctgtgt
      121 ctcgatctct gcgctgccgc cttttaacgg gtttgtttcg gaatggctga cctaccaggc
      181 cttcctgctt tcgcccgcac tccccagtcc gctcttgaac ctcctcattc cgctgggggc
      241 cgctctcctg gcgctgacga gcgcgcttgc cgctgcctgt tttgtaaagg ccttcggcgt
      301 taccttcctg ggtcatcagc gcggacacca tcatggccgt atccacgagg cggactggtc
      361 catgcgtatc ggcatgctgt tggctgcgct cacctgcctg gccctcggcg tcctgcccac
      421 ggtgatgatc cgctggatgg acccgctcgc cgaggaactg gtcggcggca cgatagccgc
      481 ctccgcgtcg ggaatcggat ggatgtggct cacgccggtt gcggccgagc gtgcctccta
      541 tgcggcgccc atcgccttca tgggcatcct gtccgttgtt gtcgtgacct accttctgct
      601 tcattcccgt cccggcgcca tccaccgagt gccgctctgg gactgcgggt ttgaaaagat
      661 caccagccgg atgcagtata cggccacgtc tttctccatg cccctgcgca ggattttcgg
      721 atttttgttc agcatcaagg agcatgtccg gcagatcccg cccgtctcgc atccggcgtt
      781 cccgcagcgc ctgcagtacc atctccgggt gcgggaccgt ttctggatgt ggatgtacca
      841 gcccgtggtc gacgcaagtt tctggctgtc gcgccggatc gggaggatgc agcaggggag
      901 gatccagatc tacctgatct attcgttcgt gacgattctt gtgctgctga tattttcgag
      961 gtgaaggatt ttgacagata aaaaattggt cagccatgaa ttaatcatgg atcaattcat
     1021 gtcataaaac aggaatattt cgcgcggaga cgcggaggcg cagagaaaag aatgctttgt
     1081 ttttttataa ctcttgaagg attctgacgt tttctgcgta ccctgcgact ctgcgcgaaa
     1141 ctggtctttt tattcctttt catccgggtt agactttaaa tctgattcaa gcctaggatt
     1201 ctgtgaaatt atgaggcgag gccttcattg aacatccatc cgctcatcat agaagttctt
     1261 caggtcgccg tcctcctctt cgtcgccccg ctgtttgcgg ggtgggtgaa gatggtgaag
     1321 tgctggttcc agggacgctc ggccccgagc ctgttccagc gttaccgcga cattaggaaa
     1381 ctcctggcca aggacgtgac gctggcggag aacgcatcct ggatcttccg ctttaccccg
     1441 tacctcgtct tcggcgtctc ggtcatggca ggcgggatca tccccatcct gtccgtcgat
     1501 ctgccgctgt cggcaaccgc tgacgtcatc gccctggtgg ccctttttgc cattgcccgg
     1561 ttctttacgg ccctggcggg catggacatc ggcaccgcct tcggcgggat ggggtcgagc
     1621 cgtgaaatga ccatcgcctc ccttgccgag cccgcaatgc tcatggcagt cttcgccgtt
     1681 tcgctggtga gccattccac gtccctgtct cacatgaccg ctgtcatatc ccaggggcat
     1741 tccctgctcc ggccctcgct ggtcttcgcg ctcctggcgt tcgtcctcat tgcccttgcc
     1801 gagacgggga gggtgcccgt ggacaacccg gccacccatc tggaactcac catgatccac
     1861 gaggccatga ttctcgaata ttcgggcagg cacctcgcgc tcatcgaatg ggcgaacatg
     1921 atgaaactgt ttctctttac agccctcggg attgccgcat tctttcccgg cgggatcgcg
     1981 cctgacgacg atctgctggc cgtcgcggcc gcactcccga tcctcctgat caagctcgcg
     2041 atcgtcgggg tggggctggt gacgattgag acgggactgg caaagatgcg aatcttccgc
     2101 ataaccgagt ttttggggag cgcctttctc ttcgcgaccc tggggatgtt gtcgtatttc
     2161 atgctggaat agaaggcaag caccaaatga caaacaccaa atttcaaata atatccaagc
     2221 accaaggctc gaaacttcaa tcgtttcgat cattggtcct ttgaatattg gaatttattt
     2281 gggatttgga atttgttatt tggaatttac ttgatttgta gaggaatccc atgaacctgc
     2341 accttgcggc tcaaatcaac agtcttctgg cggcaatgat cctgcttacg gcctttgccc
     2401 tggtcgtgca gcgcaggatg aatgcgcttc tgcatctctt cgcgtggcag gggctcttgc
     2461 ttgcggtgag caccgccatc gtcgggtacg tcggcggaca gcacgatctg tacatctcct
     2521 ccgtcctgac gctttcgatg aaggtcatcc tgctcccgta catcctgcac aagctcatcg
     2581 tcaagctcag aatccacaag gaagtggaga cggtggtgaa cattcccacc accatgctga
     2641 tgggaatcgt cctggtcata ttttcatacc acctcacagc ccccatcagg gagctctcga
     2701 cgctcgtgac gcgttcgacc ctggctgtag ccctcgccac agtcatgctc ggcttcctga
     2761 tgatgatcac cagaaagcac gcggtcaccc agatcatcgg gttcctcgct atggagaacg
     2821 ggctgctctt cgccgccacc agcgccacct acggcatgcc ccttgtggtg gaattgggcg
     2881 tagccctgga ccttctgatc gccgcgttta tcttcgggat gttcttcttc catatcagca
     2941 cgacgttcga cagcctcgac gtggaccaga tggcgaggct gaaggaagaa gattaaattt
     3001 cggaaggtgg gaagttggga aggtgggttt tctgttccca ccttctcaac ctctgacgtt
     3061 ctcaacatcg aggtttttat ggagcttctc gtactcttag gcgtgccgct cgtcgcggcc
     3121 ctggcgcttg cattcgtcgg cgaccggaag ctggctcccg aggtgaatat cctcggctcg
     3181 gccgcgacct tcatcgcgag cgtgaagctt gcgctcgagg tctacgggcg cggatcgatc
     3241 ctggcgggga acaacttttt cttcgtcgac gccttcagcg tctatctgac ggtgctgacc
     3301 gcgttcgtct ccatgaccac cgccatcttc agcaggcgct acatgcggag ggagcgcgag
     3361 cacggcaccg tcggtcaccg gcgtctgcgc ttctaccact ccatgtacca gctcttcatc
     3421 ttcgccatgc tgctctgttt gctcacgaac aacgtgggca tcctctggat cgccatggaa
     3481 ctggccactc tgtccacggt gctcctggtg tccctgtacc gcacgcccgc ggccatcgag
     3541 gcggcatgga agtatttcat cctctgcggc gtggggatcg ccctggcact cttcggcacg
     3601 gtgctcctgt acttcgccgc ggaaaaggtg ctcggcgaag gcggggccgc tctccattgg
     3661 acgaacctgt atcaggtcag caatcagctt gaaccgacca tcctgtccct tgccttcgtg
     3721 tttctcatgg tgggctacgg cacgaaggtt gggctcgtcc cgatccacaa ctggctgcct
     3781 gacgcgcaca gcgaaggccc cacgcccatc tcggccgtgc tctcgggact tctcctcaac
     3841 atcgcgctct acgcgctcgt acggtgcaag gtgctcgtgg acggctccac cggcacgcat
     3901 cattcgggcg atatcatgat gggcttcggc gtcatctcga tcatcgtcgc ctctttctcg
     3961 ctcctgcggc agaaggacat caagcggctc ttcgcctatt cctccatcga acacatgggc
     4021 atcgccacct t
//
LOCUS       DASSLD010000033         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_484221,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000033 DASSLD010000000
VERSION     DASSLD010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484221"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFK23_00560"
     CDS             complement(<1..237)
                     /locus_tag="VFK23_00560"
                     /inference="COORDINATES: protein motif:HMM:NF025719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HET7317603.1"
                     /translation="MNLKQHLKEKKSEILKQWFDFVAGTYPDETSTFLKKKKAQFANP
                     VGFTLEDGLEHLFDALLAGMLPDTVSRFLDSIVRI"
     gene            complement(304..717)
                     /locus_tag="VFK23_00565"
     CDS             complement(304..717)
                     /locus_tag="VFK23_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HET7317604.1"
                     /translation="MGIVKVLFVVQRPPYKSENPRLALTHALASQTAEIYMDDGDAVV
                     PTVVFVGDGVLNAVKNQQAMKLYGVTSTESHIKNSLLIDLPIWVCKDDMDRLGLKEDQ
                     MITDAEEIGGELKTKFVSYADIQKEMEAVQHLLFF"
     gene            complement(718..1077)
                     /locus_tag="VFK23_00570"
     CDS             complement(718..1077)
                     /locus_tag="VFK23_00570"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HET7317605.1"
                     /translation="MGVLTIGSFPSLVGSMTLDFTFKLAEAAIAKGHHVNVWLSGNAV
                     MMSKKGQKAFKDYSHLQKRMNELIAKGMEITVCEACAEARGYHKEDTPEGMKRASMDW
                     YLAETVKTDKLLHIGGE"
     gene            complement(1118..1417)
                     /locus_tag="VFK23_00575"
     CDS             complement(1118..1417)
                     /locus_tag="VFK23_00575"
                     /inference="COORDINATES: protein motif:HMM:NF016003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsrH/TusB family sulfur metabolism protein"
                     /protein_id="HET7317606.1"
                     /translation="MKLAVFLSDWRRTEDTFERMKADNLGVILVGNGIYHAVVKENGK
                     DSPVLSKAGATFYVLSDDLETRGYTAANVNPKVKVIGYGDIVDLIMNDYEKSAWL"
     gene            complement(1506..1691)
                     /locus_tag="VFK23_00580"
     CDS             complement(1506..1691)
                     /locus_tag="VFK23_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET7317607.1"
                     /translation="MYMVTIDKNKCDGDGTCVNVCPQSVFKLDGGKADPVNMSECINC
                     QTCVENCPQQAITVTEI"
     gene            complement(1891..2226)
                     /locus_tag="VFK23_00585"
     CDS             complement(1891..2226)
                     /locus_tag="VFK23_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HET7317608.1"
                     /translation="MGTITVKGKTIEVDEDGFLANLDDWNLDVANYFAEVEGIAMSDQ
                     HWEVVNFLRDYYKQYQIAPMIKILVKEIGKKMGPEKGNTKYLYELYPGGPAKQACKIA
                     GLPKPTGCV"
     gene            complement(2321..>2570)
                     /locus_tag="VFK23_00590"
     CDS             complement(2321..>2570)
                     /locus_tag="VFK23_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317609.1"
                     /translation="CSCGAVYIADPTGKSVGEVMMQALSLAAEKLSKDITELVAGEDY
                     EDAILSYDWRTHRSPGLSKGFMDGYGRMYVLKVKRKTS"
BASE COUNT          501 a          759 c          648 g          662 t
ORIGIN      
        1 gatcctgacg atgctgtcga gaaaccggga cacggtgtcg gggagcatcc ccgcgagcag
       61 cgcgtcgaac aggtgttcga gcccgtcctc aagcgtaaaa ccgacagggt tcgcgaactg
      121 ggcctttttc ttttttaaaa atgtggaagt ctcgtccgga taggtcccgg cgacgaaatc
      181 gaaccattgt ttcaggatct cggatttctt ttctttcaga tgctgtttca gattcatgtt
      241 ccttttacca caaagcagtc atccccgcga cgcagtgccg cggggatttc gactattcat
      301 gtattagaag aacaggaggt gctgtaccgc ctccatctcc ttctgtatgt ccgcatagga
      361 cacgaacttc gttttcagtt cgccgccgat ctcctcggcg tcggtgatca tctggtcctc
      421 tttgagaccc agacgatcca tatcgtcctt gcatacccaa atgggcagat cgatgagcag
      481 gctgttcttg atatggctct ccgtgctcgt aaccccgtag agcttcatcg cctgctgatt
      541 ctttacggcg ttcagcacgc cgtcgccgac aaacactacc gtcgggacca cggcgtcgcc
      601 gtcatccata tagatttcgg ccgtctggct tgcgagcgca tgggtaaggg caagacgcgg
      661 gttttcgctc ttatacggcg gccgctgaac aacgaacaat acttttacga tgcccattta
      721 ctcacctccg atatgaagca gcttgtcggt cttgacggtc tctgccaggt accagtccat
      781 gctggctcgt ttcatgccct cgggcgtgtc ttccttgtga tatccgcggg cttcagcgca
      841 ggcttcgcag accgtaatct ccatgccctt ggcgataagt tcgttcatgc gtttctgaag
      901 gtgcgaataa tccttgaatg ccttctgacc cttcttggac atcatgactg cgttgccgga
      961 cagccatacg ttcacgtggt ggcccttcgc aatcgcagcc tcggcgagct tgaaggtaaa
     1021 atccagcgtc atcgagccga ccagggatgg gaacgatcca atcgtgagta ctcccatagt
     1081 gctcctcctt aaataataaa agtgtcgtgt gctgatgcta caaccacgcc gacttctcgt
     1141 agtcgttcat gataagatcg acgatgtcgc cgtagccgat gaccttcacc ttggggttga
     1201 cgttagcggc agtatagccc ctcgtttcca gatcatccga cagcacgtag aaagttgccc
     1261 ctgccttgga aagcacggga gagtccttcc cgttctcctt gacgacggcg tgatatatgc
     1321 cgttgccaac gaggatgacg cccaggttgt cggccttcat tctttcaaag gtgtcttcag
     1381 tccttctcca gtcacttaaa aaaaccgcga gcttcatctt ctcacctccc cgagttctcg
     1441 ttcaggataa aaaaacaggg gatagattgt tcaactatcc ccttgtatgc aggtcattga
     1501 gtgcatcaga tctcggtgac cgtaatggcc tgctgcgggc agttctccac gcaggtctgg
     1561 cagtttatgc attcggacat gttcaccgga tcggccttgc cgccgtccag cttgaacacg
     1621 gactgcggac agacgttcac gcacgtaccg tcgccgtcgc acttgttctt atcaatggta
     1681 accatgtaca tggcggctca ttcctcccca cgccgtcggg cgtgtactca ctgatcatct
     1741 gaagttcact gctttccggc cgcccgtgca tctgcatgga catacggtga cagccggcag
     1801 acgcacctcc cgcgtacgat atagccctgg cgggttccgc gcagatttct caggatgatc
     1861 gcaacgcgag agcgggcagt cggacagatg ctatacgcag ccggtcggtt tgggaagacc
     1921 ggcgattttg cacgcctgct tcgccgggcc gcccggatag agctcataga ggtacttggt
     1981 gttgcccttt tcgggcccca tcttcttgcc gatttccttc accaggatct tgatcatcgg
     2041 cgcgatctgg tattgcttat agtaatcgcg gaggaaattc acgacttccc agtgctggtc
     2101 gctcatcgcg atcccctcga cctctgcaaa gtagttggcg acatccaggt tccagtcgtc
     2161 caggttcgcc aagaaaccgt cctcatccac ttcaatcgtc ttgcctttta ctgtaatcgt
     2221 tcccatttgt ctgtgtcccc tttcgttaag atgttcgggc gccttgtgag cccccacatt
     2281 tgttatcgtg tctgtcgtcg acggccggtg gccggcccgt ctacgacgtt tttcgcttca
     2341 ccttgagcac atacattcgc ccgtatccat ccataaagcc cttgctgagg ccgggtgacc
     2401 ggtgcgtccg ccaatcgtag ctcaatattg cgtcttcgta atcttctccg gcaaccagct
     2461 cggttatgtc tttcgaaagt ttctccgcgg caagactcag cgcctgcatc atcacttcac
     2521 ccacgctctt gcccgtcggg tcagcaatgt agactgcgcc gcaggagcac
//
LOCUS       DASSLD010000034         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1397765, whole genome shotgun sequence.
ACCESSION   DASSLD010000034 DASSLD010000000
VERSION     DASSLD010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397765"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..730)
                     /locus_tag="VFK23_00595"
     CDS             complement(107..730)
                     /locus_tag="VFK23_00595"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HET7317610.1"
                     /translation="MMTARAQAAAKPGRGRGLFSFIAAFVLGAALIAGCAGKDFEALR
                     PDIKARGVYIEGVPFYRQTDSACGPAALAGVFAFWGKPLAVDKIAEKTYLPELRGTLP
                     MDMESFARDAGFNTMSTNGTLTVLKEHIRRGTPVICLLDLGFGPYRQPHYVTAIGFED
                     AHAVIIMHDGLKPDRLIGYESFDRAWSRAGRWMLVIKPEKTEKKHES"
     gene            complement(727..1185)
                     /locus_tag="VFK23_00600"
     CDS             complement(727..1185)
                     /locus_tag="VFK23_00600"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HET7317611.1"
                     /translation="MNNNEKETDIMRPMLTSFYTKPLVAYLVIALLSLSSFAGPAEAM
                     FLPASPLQDATGATAALSARTADLAKIQAALESKVLRQKLMDYGLSPEEAMSRLDKLS
                     DEQIHQLAANTDSLQAGGDALGFLVGVVILAILVVLLIYLLEGRIEVRHR"
     gene            complement(1192..1575)
                     /locus_tag="VFK23_00605"
     CDS             complement(1192..1575)
                     /locus_tag="VFK23_00605"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HET7317612.1"
                     /translation="MRKKSCFKILSVYLMFAMIMLSLPAHGWAMFLHSDHAASVRQAD
                     MISIQKALETTVVKQRLKDLGLSPEETLARIDSLSDEQVHQFAGNLDSLQAGADGLGA
                     LIFLVLVVILVVVILEATGHRVIVR"
     gene            1802..>2587
                     /locus_tag="VFK23_00610"
     CDS             1802..>2587
                     /locus_tag="VFK23_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7317613.1"
                     /translation="MTICRGMIAVLTAAALFGCVTTKEYNARLADIEGLKGNVSSLQE
                     KLIETEADKATAQAEADGLQKELDELKKQNKALQDDNANLDGLLKAKKDELSAKIAGL
                     RDTLAEKELQIESLKTKIAELNREKEAAVQEKERSVAEMKKTYDSLMGEMNQEIKKGE
                     ITITQLKDKLSVNLVERILFDSGSAEIKKDGKKVLDRVAEILAKVTDKEIRVEGYTDN
                     VPISPRLAARFPTNWELSTARATTVARYLQDKGIDPTILSAAGY"
BASE COUNT          624 a          696 c          771 g          496 t
ORIGIN      
        1 aagggggtcg ttgaggatga tgatccgggg aagagaacag cctgagagaa cgaccatgac
       61 ggcgacgacg aggaaaaacg gtgacttgcg gactccggag gcggaattac gactcatgct
      121 ttttctcggt tttttccggc ttgatcacca gcatccagcg gccggcccgg gaccaggctc
      181 tgtcgaacga ttcatagccg atcaaacggt ccggtttcaa tccgtcatgc atgatgatca
      241 ccgcatgggc gtcctcgaat ccgatggcgg tgacataatg cggctgacgg taaggtccga
      301 atccgagatc gagcaggcag ataacgggcg tcccgcggcg gatgtgctct ttcagcacgg
      361 tgagcgtccc gtttgtcgac attgtattga accctgcatc gcgcgcaaag ctttccatgt
      421 ccatcggcag ggtgccgcgg agttccggaa ggtatgtttt ttcggcgatc ttgtcgaccg
      481 ccaaaggctt tccccagaac gcaaatacgc ccgcaagggc ggccggaccg cacgcggaat
      541 ccgtctgccg atagaaaggg accccttcga tatagacgcc ccgcgccttg atgtcgggcc
      601 tgagggcctc aaagtctttt ccggcgcatc cggcgatgag agctgccccc agcacgaagg
      661 ccgcgatgaa tgaaaataat ccgcggccgc gccccggctt ggcggccgcc tgagcgcggg
      721 ccgtcatcat ctgtgccgga cctctatgcg gccttccagc aggtagatga gaagcacgac
      781 aagaatggcg agaatgacga cgccaacaag aaatccgagt gcatctccgc cggcttgaag
      841 ggaatctgtg ttcgcggcca gctgatggat ctgctcatcc gagagcttgt ccagccgcga
      901 catggcctcc tccggagaaa gcccgtagtc catgagcttt tgccggagca ccttcgattc
      961 cagtgcagcc tggatttttg ccaggtccgc ggtccgggcc gataaggccg ccgttgcccc
     1021 ggttgcatcc tggagaggcg atgcagggag gaacatggct tcggcgggtc cggcgaagga
     1081 agagagggaa agaagggcga tgaccagata ggcgaccagc ggtttcgtgt agaaagaggt
     1141 aagcatgggt ctcatgatat cggtctcctt ttcgttatta ttcatcgtgc atcatcggac
     1201 aatgaccctg tgccctgttg cctcaaggat tacaaccaca aggatcacca cgagtacaag
     1261 aaagatcaac gcgccgagcc cgtcagcgcc cgcctgcagc gaatccaggt tcccggcgaa
     1321 ctggtgaacc tgctcatcgg aaaggctgtc gatccgagcc agggtctcct ccggggaaag
     1381 ccccaggtcc ttgagccgct gtttgaccac cgttgtttcg agcgcctttt gaatgctgat
     1441 catgtcagcc tgtctcaccg aagcggcatg gtccgaatga aggaacatgg cccatccgtg
     1501 cgccgggaga gagagcatga tcatcgcaaa catcaaatag acagaaagga tcttgaagca
     1561 ggattttttc cgcatcacat tgacctcctc acatgtcatt gtttacaccg catcgtataa
     1621 atgtcagtta ttatatcaga atatgtattt tttgtcacag cgaaaaatgt gcgcctgcgc
     1681 cgttgatgac agccgccgcg atagttgcta taatagcagc attgccgtga cggggagatt
     1741 ttttaaccgt aatgtatctg ttgctgtctc gggatttcaa cgtcaaagga ggtttcttgt
     1801 catgacgata tgcagaggca tgatcgccgt tctcaccgcg gcagccttat tcggatgcgt
     1861 gacgaccaag gagtacaatg cccggcttgc ggacatcgag gggctcaaag gcaatgtttc
     1921 gtccctgcag gagaaactga tcgagacgga ggccgacaag gccacggccc aggcggaggc
     1981 ggacggcctg caaaaagagc ttgatgagct aaagaagcag aacaaggccc tccaggacga
     2041 caacgcgaac ctggacggcc tcctgaaggc gaagaaggac gaattgagcg cgaagatcgc
     2101 cggactccgg gacacattgg cggagaagga acttcagatt gaatcgttga agacgaagat
     2161 cgccgagctg aaccgggaaa aagaggcggc cgtccaggag aaagaacgtt ccgtggcgga
     2221 gatgaagaag acctatgaca gcctcatggg cgagatgaac caggagatca agaagggaga
     2281 aatcaccatc acccagctca aggacaaatt gtccgtcaat ctcgtcgaga gaattctgtt
     2341 tgattccggg agcgcggaaa tcaagaaaga cggcaagaag gtcctcgacc gcgtcgcgga
     2401 gatcctggca aaggtcacgg acaaggagat cagggtcgag ggttacacgg acaatgttcc
     2461 gatcagcccc cggctggcag ccaggttccc gacaaactgg gaactctcca ctgcccgtgc
     2521 aacgaccgtg gcgcgatatc tccaggacaa gggcatcgac ccgacaatcc tgagcgctgc
     2581 cgggtat
//
LOCUS       DASSLD010000035         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_680310,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000035 DASSLD010000000
VERSION     DASSLD010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680310"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VFK23_00615"
     CDS             <1..445
                     /locus_tag="VFK23_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7317614.1"
                     /translation="GFGDVMRHMHVNAWTPLVLSREFARLARRGTIINILDTKIAGFD
                     RKHAAYILSKHMLSVVTRLCAVEFAPEITVNGIAPGLILPPNGKDETYLDKKAKALPL
                     KRHGGPEDVTEAALYLLRSRFVTGQVIYVDGGRHLLEADNGPNPD"
     gene            426..788
                     /gene="folB"
                     /locus_tag="VFK23_00620"
     CDS             426..788
                     /gene="folB"
                     /locus_tag="VFK23_00620"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET7317615.1"
                     /translation="MDRILISDLLVRCIVGINDDERREKQDVLINLALSADLSRAGRS
                     DRFEDAVDYRAIKKKVAAMAENSRFFLVEALAEAVAAICLEHPAVSEVLVRVEKPTAL
                     RYARSVGVEITRRQPGRS"
     gene            785..1312
                     /gene="folK"
                     /locus_tag="VFK23_00625"
     CDS             785..1312
                     /gene="folK"
                     /locus_tag="VFK23_00625"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET7317616.1"
                     /translation="MTRAFVSIGSNIDPEENVKKAVLLLAGRTTVRAVSTVYLTAAED
                     RPEQPPYYNCVVEIETEKPPVEVKQGILRRIEAELGRVRTRDRYAARTIDLDLVLYGD
                     LVTKTEDLTLPDPDIVRRPYLAVPLEELAPGLLMPGSGMPIHEVASALSRKAMNPLEG
                     YTELIRKELTHERKQ"
     gene            1296..1871
                     /gene="folE"
                     /locus_tag="VFK23_00630"
     CDS             1296..1871
                     /gene="folE"
                     /locus_tag="VFK23_00630"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET7317617.1"
                     /translation="MSGNSERIQSLIRELLVELGEDPAREGLVDPPKRVAASLAYLTS
                     GYQADVEKLINQACFEQETTSMVVVKDIEMYSMCEHHMLPFYGRCHIGYIPRGRVFGI
                     SKLARLVDMFSRRLQIQERLTEQIAYAIKDSINADGVGVMIEARHLCMMMRGVEKQNS
                     MFVTSSVLGVFRTNPATREEFFSLISRPRSI"
     gene            1882..>2055
                     /locus_tag="VFK23_00635"
     CDS             1882..>2055
                     /locus_tag="VFK23_00635"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET7317618.1"
                     /translation="MNIEILTIGHSTRPVEEFIDILTAHGVQLVADVRTIPRSRHNPQ
                     FNSDALADRLKASG"
BASE COUNT          434 a          636 c          614 g          371 t
ORIGIN      
        1 cggcttcgga gatgtcatgc ggcacatgca cgtgaatgcg tggacgcccc tggtcctttc
       61 ccgggagttc gcgcgactgg cgcggcgggg aacgatcatc aacatcctcg acacgaagat
      121 cgccggcttt gaccggaagc acgcggcgta cattctgagc aagcacatgc tctcggtcgt
      181 gaccaggctg tgcgccgtcg aatttgcgcc tgaaatcacg gtaaacggaa tcgcgcccgg
      241 cctcatcctc cctcccaacg gcaaagatga gacatatctc gacaagaagg ccaaagccct
      301 gccgctcaaa cgtcacggcg gtcctgaaga cgtgacggag gccgcgctct atctcctccg
      361 tagccgtttc gtcaccggtc aggtcatcta cgtggacgga ggcaggcacc ttctggaggc
      421 ggacaatgga ccgaatcctg attagcgatc ttctcgtacg gtgtatcgtc gggatcaacg
      481 acgacgagcg tcgggaaaaa caggacgtgc tgatcaatct ggccctctcc gctgaccttt
      541 cccgggcggg caggagcgac cggttcgagg acgcggtgga ttaccgcgcc atcaagaaaa
      601 aggtcgcggc catggccgaa aactcccgat tctttctcgt ggaagcgctg gccgaagccg
      661 tggccgcgat ctgtctcgaa catccggccg tctcggaagt cctcgtaagg gtagaaaagc
      721 ccacagccct caggtatgcc cggagcgtgg gggtggagat cacgaggcgg cagccgggcc
      781 gatcatgacg cgtgcctttg tcagcatcgg atcgaacatt gatcccgaag agaatgtaaa
      841 aaaggccgtc ctcctcctgg ccggccggac cacggtccgc gcggtctcca cggtctacct
      901 cacggcggcg gaagacaggc ccgagcagcc cccttattat aactgcgtcg tcgaaatcga
      961 aaccgaaaag ccaccggtcg aagtgaagca aggcatcctg cggcgcattg aggctgaatt
     1021 ggggcgcgtg cggacgcggg accggtatgc ggccaggacc atcgatctcg accttgttct
     1081 ctacggcgat ttggtaacga agacggagga cctgacgctt ccggacccgg acatcgtcag
     1141 gcggccgtat ctcgcggtcc ctcttgagga gcttgcgccg ggactgctga tgcccggctc
     1201 gggcatgccg attcatgagg ttgcttcggc tttatcgcga aaggcgatga atccgcttga
     1261 aggctacaca gaactcatca gaaaggaact gacccatgag cggaaacagt gaacgcatcc
     1321 agagtctgat aagggaacta ctggtcgaac tcggcgaaga ccccgcgcgc gaggggctcg
     1381 tcgatccgcc caagcgcgtc gcggcctccc tggcgtatct cacctcgggc tatcaggcag
     1441 acgttgaaaa gctgatcaat caggcctgct tcgagcagga gacgaccagc atggtcgtcg
     1501 tcaaggacat cgagatgtac agcatgtgcg agcaccacat gctgcccttc tacggccgct
     1561 gccacatcgg ctacatcccc cggggcaggg tcttcggcat cagcaaactc gcccggctcg
     1621 tggacatgtt cagccgccgc ctccagatcc aggagaggct caccgaacag atcgcctacg
     1681 cgatcaagga ctcgatcaat gccgacggcg tgggtgtcat gatcgaggcg cgccacctct
     1741 gcatgatgat gcggggcgtc gagaagcaga attccatgtt tgtgacctcc tcggtcctgg
     1801 gcgtcttccg gacgaacccg gcgacccggg aggagttttt ctcgctcatc agcagaccga
     1861 ggtcgatttg atcagccgca gatgaacatc gaaattctca ccatcggcca ttcaacccgt
     1921 cccgtcgaag agttcatcga catcctgacg gcgcacggcg ttcagcttgt tgcagacgtg
     1981 cggaccattc cccggtcccg ccacaaccct caattcaaca gcgacgcgct ggcagaccgc
     2041 ctcaaggctt ccggc
//
LOCUS       DASSLD010000036         3919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_158445,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000036 DASSLD010000000
VERSION     DASSLD010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158445"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..517)
                     /locus_tag="VFK23_00640"
     CDS             complement(41..517)
                     /locus_tag="VFK23_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7317619.1"
                     /translation="MSPDTDAAVKDKHLLIAVDESDSSRRAVLYVADFLGGFPGFTVT
                     LISIIPEPEEDFFESDAAMNTWIKDKVDATNKMLANYKQVLMQSGFPEEKVRFRSCVG
                     EAKSFSDAILETRCDLTCCTVVVGRHHKTKTEEFLFGSTSNRLIHEAKNCAVWVVE"
     gene            complement(637..1620)
                     /locus_tag="VFK23_00645"
     CDS             complement(637..1620)
                     /locus_tag="VFK23_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317620.1"
                     /translation="MASGGVYSEKELLRMIRRWVPRERVPKRVRIKDTEDFFRVDYDD
                     VVVLEGIPYFIRNNEREGRFGLDDEQKFWVKRARNLLNGDVKIVKWTFRERFNAKIGN
                     LVFECVRSPQKEARILELVRGRPEFMQGKTVLDSSGNQLRILDYIQGKTLGQIAVELG
                     SGHEDYFYNFYPALVDEFVRIVDAIRFLHENNEKHGDIRRDHIIKDDATRRYRWIDFD
                     FNYRHGESRFGLDLFGLGNVLLFLTGRGDITTHRLLREDPGLLERLSYDDVNIVFHNR
                     VNNLKKIYPYIPEKLNRVLMHFAASAPVYYEETRELLEDLGEAREELNAGR"
     gene            1941..2660
                     /locus_tag="VFK23_00650"
     CDS             1941..2660
                     /locus_tag="VFK23_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET7317621.1"
                     /translation="MDLLLHLIKKHEIDIYSIPIALITQQYLEYIELMKSLDMEIAGE
                     FLVMASSLTHIKSKMLLPPSENTEGGEDGADPRAELIRRLLEYKSFKEAAASLENKEE
                     TWSRVFTRQAEPAPEPPAEDEPLLFDFHLFDLLAALRDVMARVPEAGFEITTEAVSIT
                     EKISQILARLEAVDSLLFADLFERSTSKAQVIGTFLALLELIKTRIVRAVQIEQFGAI
                     RIIKAVTEPQNKDTSTGNLFG"
     gene            2793..3641
                     /locus_tag="VFK23_00655"
     CDS             2793..3641
                     /locus_tag="VFK23_00655"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET7317622.1"
                     /translation="MKPDIKALAQGKKFGGIEAAVYADRSLIGDLVSLLGEKDGDIRS
                     GVLNLLSRVGFDYPHDTRPHLPVLIALLDDPDMFIKIDAANAVGNLGFSFKDSASQAA
                     PKLKDLLNHDQSLVRQEAAYALGNIGFAHTDLVRDAVPRLIRMLEGGDDDEREAAAHA
                     LGKVGINAPELIRGILPKLVEVLEFSKEATCGGVLFALGAIGLHYPQEVAALVPKLMT
                     YLDSVHPNAVKNAVAALGNIGSTNPDLVRDALPKIRLILETSTDIDIKMNAKVALGRM
                     GDPAAM"
     gene            3730..>3919
                     /locus_tag="VFK23_00660"
     CDS             3730..>3919
                     /locus_tag="VFK23_00660"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET7317623.1"
                     /translation="MEDQQLKSAIEAILFVAGNPLSAERLTGLFEEEDAGRIEAQLAS
                     LAQEYDSRGSGIMLAEVAG"
BASE COUNT          827 a         1138 c         1127 g          827 t
ORIGIN      
        1 ccattcgtgc ctttccgggg tctataaaga tacgaagatt tcactccacc acccacacgg
       61 cgcagttttt cgcttcgtgg atgaggcggt ttgacgtgct gccgaagagg aactcctcgg
      121 tcttcgtctt gtggtggcgg ccgacgacaa cggtgcagca tgtgaggtcg cagcgggtct
      181 cgaggatcgc gtcggaaaag gactttgcct ctcccacaca ggaacggaag cgcaccttct
      241 cttcggggaa cccggactgc atcaggacct gcttgtaatt cgccagcatc ttgttggtag
      301 catcgacttt atccttgatc catgtgttca ttgcagcgtc gctctcgaag aagtcctcct
      361 cgggttcggg gatgatcgag atgagggtga ccgtgaaccc cgggaacccc ccgaggaaat
      421 cggcgacata caaaacggcg cggcgcgagc tgtccgactc gtcgacggcg atcagcaggt
      481 gtttgtcctt cacagcggca tcggtatcgg gtgacatggt atcactcctt tcgacaaatc
      541 tcgaaggtga gagggtagga gagtgcgaac gtgggaaggc atagatatac ttcccctctt
      601 cgactcctct ttcaaccctc taatcttccc accttcctac cttccggcgt tcaattcctc
      661 cctcgcctcg ccaaggtcct cgagcagttc cctcgtttcc tcgtagtaga ccggcgcgct
      721 tgcggcaaag tgcatcagga cccggttcag cttttccggg atgtagggat agatcttctt
      781 gaggttgttg acccggttgt ggaatacgat gttcacgtcg tcataggaaa gccgttcgag
      841 aagccccggg tcctcgcgca ggagacggtg tgtggtgatg tcgccgcggc cggtcaggaa
      901 aagcaggaca ttgccgagtc cgaagaggtc caggccgaag cggctctcgc cgtgacggta
      961 attgaagtcg aagtctatcc agcggtacct gcgcgtcgcg tcgtctttga tgatgtggtc
     1021 tcgccggatg tccccgtgct tctcgttgtt ctcgtggagg aaacggatgg catcgacgat
     1081 ccggacgaac tcatccacta gcgcggggta gaagttgtaa aagtaatcct cgtggccgct
     1141 gcccagttcg accgcgatct ggccgagggt cttgccctgg atgtagtcca ggatgcggag
     1201 ctggttgccg gaggaatcga ggacggtctt gccctgcatg aattcgggcc tgccgcgcac
     1261 gagttcgagt atccgcgcct ccttctgcgg gcttcgaacg cactcgaaca cgaggttccc
     1321 gatcttcgcg ttgaaccgct cccggaaggt ccacttcacg atcttcacat cgccgttcag
     1381 gaggtttctg gcccgcttga cccagaactt ctgttcgtcg tcgaggccga acctgccttc
     1441 gcgctcgttg ttccggatga aataaggaat gccttcgagg acgacgacat cgtcatagtc
     1501 gacgcgaaaa aagtcctccg tgtccttgat ccgtacgcgt ttgggcacgc gttcgcgcgg
     1561 cacccatcgg cgaatcatgc gcaaaagttc tttttcggaa taaacgccgc cggaagccat
     1621 ccattacccc ccgcctgcta ccccccatta taccagaccg ctcaaatgcg gaatgaggaa
     1681 ttcggaatgc gaaaggcaaa ccgcccctca ccgccccctc tctttttgga gcgcggcgca
     1741 gaagaacaaa cgttcttcct tttcctcgat tttttattcc gcattccgca ttcgaggaat
     1801 gttttctctt gacatttcgg gtgaataaag ataacttctc tgaataggaa acgacatggt
     1861 ccgtaagccg acggtccatg cgcggaggtg ttcaagcacc catgtcgtac cagataaaac
     1921 tagagatttt tgaaggtccc atggaccttc tcctccacct catcaagaag cacgaaatcg
     1981 acatctactc catccccatc gctctcatta cccagcagta cctcgaatat atcgaactca
     2041 tgaagagcct cgacatggag attgccggcg agttcctcgt gatggcgtcg agtctcaccc
     2101 atatcaaatc caagatgctc ctaccgcctt ccgagaatac ggagggaggg gaggacggcg
     2161 ccgacccgcg ggccgaactc atccgaaggc tcctcgagta taagagcttc aaggaggcgg
     2221 cggcctcact cgaaaacaaa gaggaaacct ggtcgcgggt cttcacgagg caggcggagc
     2281 cagcaccgga gcctcccgcc gaagatgagc cgctgctgtt cgacttccac ctcttcgacc
     2341 tcctggcggc gctcagggac gtgatggcac gcgtacccga ggccggattt gaaatcacga
     2401 ccgaggcagt ctccatcacg gaaaaaattt cccagatcct ggcccgcctt gaggccgtgg
     2461 acagcctgct ctttgccgac ctgtttgaaa ggagcacctc aaaggcacag gtaatcggca
     2521 cgttcctggc gcttcttgag ctcatcaaga cgcggatcgt gagggccgtg cagatcgagc
     2581 agttcggagc gatcaggatc atcaaggcgg tgaccgagcc gcagaacaag gatacgtcca
     2641 cggggaatct gtttggataa gaaccggaag agctttttcc acagatcaca gagaccacag
     2701 aggaatacgg gtctgctcaa taaacgactt tattcaatta tgatttttct ctgtgccctc
     2761 tgtgttctct gtggctgacc tgatttaata ttatgaaacc cgacatcaaa gcgcttgcgc
     2821 agggaaaaaa gttcggcggt atcgaggcgg ccgtgtacgc ggaccgctcg ctcatcgggg
     2881 accttgtttc gctcctgggc gagaaagacg gcgatatccg ttccggcgtt ctgaaccttc
     2941 tgtcgcgggt gggattcgat tacccgcacg acacacggcc ccaccttccg gtgctcatcg
     3001 ccctgctcga cgatccggac atgttcatca agatcgacgc ggctaacgcc gtcgggaacc
     3061 tcgggttcag cttcaaggac tcggcttcac aggccgcgcc caagctcaag gacctcctga
     3121 accacgacca gagcctggtc cgccaggaag cggcctatgc cctgggaaac atcggcttcg
     3181 cccatacgga cctggtgagg gacgccgtgc cccgtcttat ccggatgctc gagggaggag
     3241 atgacgacga gcgcgaggcc gcggcccacg ctctgggcaa ggtcggcatc aacgcgccgg
     3301 agctgatccg cgggatcctg ccgaagctcg tcgaggtgct cgaattctcg aaggaggcga
     3361 cctgcggagg cgtgctcttt gcgctgggcg ccataggact gcactaccca caggaagtgg
     3421 cggcgttggt gccgaagctg atgacctatc tggacagcgt ccatcccaac gcagtcaaga
     3481 acgccgtggc ggcgctcggg aacatcggca gtacgaaccc cgacctggtg cgggacgcgc
     3541 ttccgaagat ccggctcatc cttgagacat cgacggacat cgacatcaag atgaatgcca
     3601 aggtggcgct cggccggatg ggcgatcctg cggcgatgtg agtgatcgca aagcgcatgg
     3661 cggaaggggg attaatgttt tacgttatgc gccctgcgct ctgccctctg cgtatttaag
     3721 gagtaatacg tggaagatca acaattaaaa tcggcaatcg aggccattct ctttgtggcc
     3781 ggcaatccat tgtccgcgga aaggctgaca ggtctgttcg aggaggagga cgcaggacgg
     3841 atcgaggcgc aactcgcctc gctcgcgcaa gaatacgaca gccggggctc tggcatcatg
     3901 ctcgccgaag tggcgggcg
//
LOCUS       DASSLD010000037         6707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_288808,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000037 DASSLD010000000
VERSION     DASSLD010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6707
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288808"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFK23_00665"
     CDS             <1..360
                     /locus_tag="VFK23_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7317624.1"
                     /translation="YAVMRLMVGLLFACHGVQKVFGMLDGQSQVSNPVLLAAGIIEFV
                     GGGLVAIGFWTSYAAFIASGEMAVAYFIAHAVKGFWPILNKGELAVLYCSVFLYIASR
                     GSGALSVDAVAKKKNRA"
     gene            complement(466..1260)
                     /locus_tag="VFK23_00670"
     CDS             complement(466..1260)
                     /locus_tag="VFK23_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7317625.1"
                     /translation="MIHLLVIFPFAVQVLAGPLKDANDAWRRDDYPTAHRLYKSLAEQ
                     GNTLAQIRIGIMYSSGVGVRKDNREAMKWYQKAVKQYRKGADKGDAASQHGLGNMYWF
                     GRGVPLDNVEAVRWWRRAAEQGYVRSQETLAFMYSRGEGVPLDKTEAKKWYGKAAEQG
                     EASDQYSLGEIYHSEKKYPEAAMWWRRAAEQGDDDAQYHLGLLYYDGQGVPQDFVLAY
                     MWLNLSASQSNESAPKNRDLAASKMTPAQIDEAQKLAREWKQKKER"
     gene            1479..1658
                     /locus_tag="VFK23_00675"
     CDS             1479..1658
                     /locus_tag="VFK23_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317626.1"
                     /translation="MKVYTVFRIDHIRHVREPVGMVMERRKVDRGNNFEGLLKLARKL
                     YPAPSPESHIVISLE"
     gene            complement(1671..2126)
                     /locus_tag="VFK23_00680"
     CDS             complement(1671..2126)
                     /locus_tag="VFK23_00680"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET7317627.1"
                     /translation="MKKATIIVVAMALWIGISSASDTTTGKDLPKEVTLGSLSNIYEP
                     VQFNHAGHVSTAGGCVDCHHQHGTVEVKTCSECHRFDPSIFKKNVIAGRVKACGTCHL
                     ASESPGRVGLKTAYHKACFKCHKSDVGSGVKNLKGCTEMCHALNTKKMK"
     gene            2608..3141
                     /locus_tag="VFK23_00685"
     CDS             2608..3141
                     /locus_tag="VFK23_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HET7317628.1"
                     /translation="MKAAAGGALLLLSGKGFPGLASAAAVSPGPKGSLSLFNIHTGER
                     LSVDYRDGRGEYNPEAVSALNRILRCHYTQQVAEMDLRVIDFLNDVDRSLGGGNDIHI
                     ISGFRSPEYNEILVRRGQGVARHSLHLQGKAIDFRIPNASLQSLREAALRLQEGGVGY
                     YPGSGFVHIDSGRFRTW"
     gene            3222..3944
                     /locus_tag="VFK23_00690"
     CDS             3222..3944
                     /locus_tag="VFK23_00690"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET7317629.1"
                     /translation="MNKHPSLCKIHYPSDSRIEWDCLRLKKGESVETRFGERWRDVLR
                     FNRVDRRHVYPGVPLKVPRRLEDVEDFTPMPAEYPGGDNEAKLILVDLEAQFLGAYEY
                     GKRVFSAPVASGEVGNETPDGEFRITAYHSAHRSTEYFIEGTDILYPMTWALRFHVNR
                     DGVAYWIHGRDMPGYPASHGCVGLYDEAMQKKYYKLPRSPEMEDARKLFEWAILPLTD
                     DGKFHILKRGPVVRIIGGTHRP"
     gene            complement(4263..4820)
                     /locus_tag="VFK23_00695"
     CDS             complement(4263..4820)
                     /locus_tag="VFK23_00695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7317630.1"
                     /translation="MIHSVCPWWVGYFLVSPLRRLVHNPSTILRSFVSEGMTVVEPGP
                     GMGFFTMELARLVGRSGRIVAIDVQPKMLVELKRRAGKAGLLDRIDARRGTGNCMGLD
                     DLKGQVDFVLAFAVVHELPSTEGFFVEASTVLKPGHRLLLAEPRFGVSEQEFASILQI
                     ADQNGFRVDSRPAIRWTRSAVLDKV"
     gene            5050..5802
                     /locus_tag="VFK23_00700"
     CDS             5050..5802
                     /locus_tag="VFK23_00700"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HET7317631.1"
                     /translation="MYNKPGVGVRKMETTRWLAALFILIIMYAGIYFYTKSVKTIATL
                     GLPVALVVFVGIIHWIKAMGAKAEEVTDRPLNARRGAVAEEAVGNRLGELPAGYFVVN
                     DFVSKRGNIDHIVISTKGILTVETKSQKGVVTCEGEMLKCDGKPFEKDFIKQAWAEAY
                     SIRDLLTEKEVCNLRPQPVIVFTEADVQVKEKVGGVKIIGIKDLHAFLEGLPNWMSKR
                     LSDGIINCLSLTQYNYMKRGPNPKVVRHNIAS"
BASE COUNT         1520 a         1814 c         1804 g         1569 t
ORIGIN      
        1 tatgcagtta tgcgtctgat ggtcggactg ctcttcgcat gccatggggt tcagaaagtg
       61 ttcggcatgc tcgatgggca gagccaggta tccaaccctg tgcttctcgc cgcgggaatt
      121 atcgagtttg tcggaggcgg cctcgtggcc ataggatttt ggacgagcta tgctgccttc
      181 attgcaagtg gcgagatggc agttgcctac ttcatagcgc acgccgtcaa ggggttttgg
      241 cctatcctca acaaaggcga gctcgctgtg ttgtactgct ccgtcttcct gtatatcgca
      301 tccagggggt ctggggccct gagtgtcgat gcagtggcca aaaagaaaaa tcgcgcttga
      361 ccacttggga tctaggttga aggcccgaca ggacccggaa tgccccgctt tgccgagagt
      421 cgggtaggcg cctgaggcga tgcagggaca tgcccaatat atgacttacc tctccttctt
      481 ctgcttccac tcccgcgcca acttctgcgc ctcgtcaatc tgggcagggg tcatcttgga
      541 ggcggctaga tccctgttct ttggggccga ttcatttgac tgtgaagccg aaaggttcaa
      601 ccacatatag gcaagaacga agtcctgcgg aacaccttgg ccgtcgtagt ataagaggcc
      661 gagatggtac tgggcatcat catctccctg ctcggccgcc cttcgccacc acatcgccgc
      721 ttcggggtat tttttctcag agtgatatat ttccccaagt gaatattggt ctgatgcttc
      781 cccctgctca gccgctttcc cgtaccactt cttagcctcg gttttatcga gggggacacc
      841 ctcgcctctg gaatacatga acgcgagagt ttcctgtgat ctgacatagc cttgctcggc
      901 cgcccttcgc caccacctaa cagcctcaac attatcaagg gggacgcctc ttccaaacca
      961 gtacatgttc ccgagcccat gctgtgatgc ggcatctccc ttatcggctc cctttcgata
     1021 ctgtttaact gccttctgat accacttcat cgcttcgcga ttatcttttc gaacaccaac
     1081 accggaggag tacataatcc caatcctaat ttgggccaat gtatttcctt gctcggcaag
     1141 agatttataa agtcggtgcg ccgtagggta gtcatctcgc ctccatgcat cattagcatc
     1201 ttttaatggg ccagcaagta cctgaacagc gaatgggaaa atgaccagaa gatggatcat
     1261 gagcaaaccg gcggcagtat ttcgcatatg ccacctcacg aggattggat tcaccccgag
     1321 tgtataacgt gggacacgat cagggaagga acctccgata tttcaggaaa tatttcccct
     1381 atgaagcgct atcttcccgg cgaaaatatt taaagacagt attgttattt ttgatatatt
     1441 gccgataacg tataaatcca acttagggag ggttggatat gaaggtctat accgtctttc
     1501 gaatcgatca catccgccat gtgagggaac ctgtcgggat ggtgatggag cgtcggaagg
     1561 tggatcgggg gaacaacttc gaaggtctct tgaaactggc gcgaaaatta tatcccgcgc
     1621 cgtcgccgga atcgcacatc gtcatctcgc tcgaataaat aaaaaggggg ctatttcatc
     1681 tttttagtat tcaatgcgtg gcacatctcc gtacagccct tcaggttctt tacgccactg
     1741 cctacatctg acttgtggca tttgaagcaa gccttatgat aggcggtttt gagtcctact
     1801 ctccccggcg attcggacgc taagtgacac gtcccgcagg ccttgaccct tccggcgatc
     1861 acgttttttt tgaagatgga ggggtcgaac cgatggcatt ccgaacacgt tttaacctcc
     1921 accgtgccgt gctgatggtg gcaatcgacg catccccccg ccgtggaaac gtgccccgca
     1981 tggttgaatt gcaccggttc gtagatgttg gacaggctcc caagagtgac ttccttcgga
     2041 aggtccttcc cagtggtcgt atcgcttgcg gaggagattc cgatccagag tgccatggcc
     2101 accacgatga tcgtcgcttt cttcatgtat ccggtctcct tccgtcagag gtttgaagac
     2161 tcagaggatg gaagagtccg cgttgcctga cgaagcatat cgaaaatatt tgattcatgc
     2221 aaaatatata tccacaaaat ttccggttcc tgcataatgt gcccattaaa actttggggc
     2281 attagatgga ttgcaaccat cgtcgctccg gcatttcctc ggtatcctgc gcgccgtgct
     2341 ttcgcgcgag acctcagcat tggtcacggc attcgtgaat aaagacctgg gccatggaaa
     2401 acggatgaga tgtcgaagca tttacaagac aaacccctca agttgttttt gcggaaagaa
     2461 taaacgatct gtagtaacgg cgagtggcca tattgtggcc ggggaaatgg tatagtgcgt
     2521 acataccttt ttccgaaata ccttttacat cctatcgttc cagtggagga accgttgtat
     2581 cgcacgcgtt tcgatcgaag gacgattttg aaggccgccg cggggggcgc gctcctcctc
     2641 ctctcgggga aggggttccc cggcctcgcc tcggcggccg ccgtatctcc cggcccgaag
     2701 gggagtctct ccctattcaa cattcatacc ggcgaacggc tctccgtcga ctatcgggac
     2761 ggaagggggg agtacaaccc ggaggcggtt tctgccttga accgcatcct gcgctgccac
     2821 tacacccagc aggttgccga aatggacctc cgggtgatcg acttcctgaa cgacgtggac
     2881 aggagcctgg gcggcggaaa cgacatccat atcatctccg ggttccggtc cccggagtac
     2941 aacgagatac tcgtccgccg gggacagggc gtagcccgcc acagcctcca cctccagggg
     3001 aaggcgatcg actttcggat ccccaatgcg agcctccagt cgctgcggga ggccgccctc
     3061 cggctgcagg agggcggagt ggggtactat ccggggtccg ggttcgtcca catcgactcg
     3121 ggaagattcc gcacctggta aagttccgcc tcccgtccgg ggcgctcctt gccgggctcc
     3181 tcctgatcct cgcgctcccc gccgcctgct gggcggtccc cgtgaacaag cacccctccc
     3241 tttgcaagat ccattacccg agcgattcac gaatcgagtg ggattgtctc cgcctcaaga
     3301 agggcgagag tgtggaaacg cgcttcggag agcggtggag ggacgtgctc cggttcaacc
     3361 gggtcgaccg gcggcacgtg tacccgggcg tccccttgaa ggttccccgc cggctcgagg
     3421 acgtggagga cttcacgccc atgcccgccg aataccccgg aggggacaac gaggcgaagt
     3481 tgattcttgt ggacctcgag gcgcagtttc tcggcgccta cgagtatggg aaacgggtct
     3541 tctccgcacc cgtcgcttcg ggggaagtcg gcaatgaaac gcccgatggg gaattcagga
     3601 ttaccgcgta ccacagcgcc caccggtcga cggaatattt catcgagggc accgacatcc
     3661 tctacccgat gacgtgggcg ctccggttcc acgtcaaccg ggacggggtc gcctactgga
     3721 tccacggccg ggacatgccc ggctaccccg cttcccacgg ctgcgtcgga ctctacgacg
     3781 aggcaatgca gaagaagtac tacaagcttc cccggtcacc ggagatggag gacgcgcgga
     3841 aactcttcga gtgggccatc ctccccctta cggacgacgg taaattccat atcctgaaga
     3901 gggggcctgt ggtacgaatc atcggcggaa ctcaccgtcc atgagtaacc ctttcggtcc
     3961 cggaggaaac ttcccggatc gggaaacccc gctcacgccc atggcgtcta cctatcagga
     4021 aagagaattt cgagcgggag gaatgaatat gagaaagaat ttcgccgtca tctcgttggt
     4081 tggtacgctt ttggttctgg cggcattcgc ggtgttcgcg tcctcccttg caaaaacagt
     4141 ttcgattgat acttccgggg taaagtgtac ttggggcaag gattggcccc cgattacctg
     4201 tacccggggt taataaacat ataacggcca atgagtgaga accgctccgc atgcgccgtg
     4261 agttagacct tatccaatac cgccgatcgg gtccatcgga tagcaggccg ggagtccaca
     4321 cgaaaaccat tttgatcggc gatctgcaag atggatgcaa actcttgctc ggatacccca
     4381 aagcgcggtt cggccagtag aagcctgtga ccaggcttta acacagtgga tgcctcgaca
     4441 aagaacccct cggtgctggg taattcatgg accacggcga atgcaagcac gaagtctact
     4501 tgccctttga ggtcatcgag gcccatacag tttcctgtgc ctcgtcttgc gtcgatccgg
     4561 tcgagcagtc cggccttccc ggcacgacgc ttgagttcca ccagcatctt cggttgtaca
     4621 tctatggcaa cgatccttcc cgagcgaccg acgagacgcg cgagttccat agtgaagaag
     4681 cccattccgg ggccaggctc aaccacggtc attccctcgg acacgaaaga ccggagaata
     4741 gttgaggggt tatgtaccaa acgccggaga ggacttacca ggaaatagcc aacccaccaa
     4801 gggcacacac tatgaatcat atttcaccac caaatccaac attacatcgg aaatccatcc
     4861 cgcccgaagg tcttttttcc cattaaagtt cttaaccgct tcttccgaaa agagatttta
     4921 agaggaggtt tggctacatt gtaccatgcg tccagaatgt gcctccgatg tttcccccct
     4981 ggacgggggg atcttaagaa ttggtggaaa gttaagaggg tggcggggaa gaaatcgacg
     5041 agagcgacgg tgtacaacaa gcccggagtg ggggtccgaa agatggaaac cacccgatgg
     5101 ctggccgcgc tcttcatcct gattatcatg tacgcaggaa tatattttta cactaagagc
     5161 gtcaagacaa tcgcaaccct tggattaccc gtcgccctcg tcgtcttcgt tgggattatc
     5221 cattggataa aagcaatggg cgccaaggca gaagaggtca cggatagacc cctcaatgct
     5281 cgccgtgggg ctgtggccga agaggctgtc ggcaatcggc tgggcgaact cccggcagga
     5341 tattttgtcg tgaatgactt tgtatccaag agagggaaca tcgaccatat cgtcatctcc
     5401 acgaaaggga tcctcaccgt cgagacgaaa agccagaagg gagtcgttac ctgtgaaggg
     5461 gagatgttga agtgcgacgg gaagcccttc gagaaggact ttataaagca ggcatgggca
     5521 gaggcatatt cgatccgtga tcttctcacg gaaaaagagg tctgtaatct gagacctcaa
     5581 ccagtgattg tgttcacgga agcagatgtg caggtgaaag aaaaggttgg aggggtgaaa
     5641 attatcggca ttaaggatct tcatgcattc ctggaagggt tgcccaactg gatgagtaaa
     5701 agactttcgg atggcatcat caactgccta tcgttgactc agtataatta tatgaagagg
     5761 ggacccaacc cgaaggttgt gcggcacaac atcgccagtt gatcccaccg aggatgtcgt
     5821 ggtatcacca cctataaaca tctagctatt cgttaaatcc agcacctctg aaccacgatc
     5881 aattccactt ccggtgttcg aagtattcga cctgccgaag cctcttttgt gcggccgcct
     5941 tggtcctata gggacctccg agatttttcc ccgactcact cacgacttta tatccaccct
     6001 tcactttccg gatcatgaag gcaccccctt cttgaaaacc acgcctatgt tcccgctttt
     6061 tccacgtgca gcccgatccc ttccgcctcg gtgagatcgt taactgtttt ctcttatgat
     6121 gttcgtaata aaaggggggg ttccccgcgt ctgaaggagt cggaattgcc gtaggaggga
     6181 accgtggtct gagatcgtcg gcaagtcctc ctctttcaaa tctcatgccg cttagggggc
     6241 cttgagttcg ttaatagtgg cccttttcgg tcctagctat cggccataag cagatattat
     6301 tcccaataag atacattttt cgccatgata gtaaattcct gggaggctaa ttcttttgat
     6361 ttcaatgtat tacgatcagc taaattacgg cacccaatat gcttcatggt atcggtagat
     6421 acattttgag ccaatgggtt aatcctttga aagttaaaat tgctgtttgc ctttttcaaa
     6481 tactacctgc cgcccgaccg gaagcccggc acgatagtga tgttgggcag cgagttggtg
     6541 ggattgtggg gatgtggcag ggggcgcctg aaggggtagc aataccaggg ataaagtgac
     6601 gggaggcaaa aatggaaaag tactatgagt tgtcatccca gggcataaga accttctatt
     6661 tcgggagcat tgccgttcat cgaaattacg taacccttgg gcaggtc
//
LOCUS       DASSLD010000038         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_223214,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000038 DASSLD010000000
VERSION     DASSLD010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223214"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="VFK23_00705"
     CDS             <1..1201
                     /locus_tag="VFK23_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET7317632.1"
                     /translation="MRYMAISLSVTVMGLIIAYLLFGAKPQEGKTLNAVLFETMTNGW
                     GRGGAAFVLVTLLSEAMLLFIAAQAGFLDGPRVLSNMALDRWFPTKFAMLSDRLVTQK
                     GILMMGGAALVTMVLTGGSVKYLVVLYSINVFITFFLSQLGMVRHWWQERGKEAHWEK
                     KLLINGVGMTLTLFILVTVTVLKFHEGGWITLLLTGTLVGIALLTRRHYRNTFKLLHR
                     LDELVNAAEASCPINPESPEGQMLKVKYDPRDKTAVLLVNGFNGLGLHTLFSIIRLFG
                     GTFKNFVFIQVGVVDAGNFKGAEEVARMKEEVKKELDRYVHYMRCHGYYASAYSGFGT
                     DVVDEVANLTPQILERFPNAILFGGQLVFPRTTFLSGVFHNYTIFAVQRRFYNQGIPV
                     VILPIRV"
     gene            complement(1320..1799)
                     /locus_tag="VFK23_00710"
     CDS             complement(1320..1799)
                     /locus_tag="VFK23_00710"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HET7317633.1"
                     /translation="MNRYSIGEIMEMAVQTEVLGRQFYLAMAEKFKKDGGLVKLFTTL
                     ADKETSHEKTFSDLKDSVPRQGTEPVQWEEVSNYMRAFIESEFFLGRGKSLPSMDHIR
                     TVDDAVKFAIGFEKETLLYFIELRTLVKEKEVIDEVINEEKSHIRWLDTFRAGMAKK"
BASE COUNT          429 a          598 c          559 g          447 t
ORIGIN      
        1 catgcgctac atggcaatct ccctctccgt taccgtcatg ggattgatta tcgcatacct
       61 tctgttcggc gcgaaaccgc aggagggaaa aaccctgaac gctgtcctgt tcgaaaccat
      121 gacgaacggc tggggacgcg ggggcgctgc tttcgtgctc gtgacgctcc tgtccgaggc
      181 catgctcctg ttcatcgcgg cacaggcggg cttcctcgac ggcccgcgcg tgctctcgaa
      241 catggcgctg gaccggtggt tccccacgaa gtttgccatg ctctccgacc ggctcgttac
      301 ccagaaaggc atcttgatga tgggcggtgc agcgctcgtc accatggttc tgaccggcgg
      361 ctccgtgaag taccttgtcg tcctgtacag catcaacgtg ttcatcacgt tcttcctttc
      421 ccaactcggc atggtgcgcc actggtggca ggagcggggc aaggaagccc actgggagaa
      481 aaaactgctc atcaacggcg tgggcatgac actcaccctg ttcatccttg ttacggtgac
      541 ggtgctcaaa ttccacgaag gcggctggat tacgctgctt ctcacgggca cgctggtggg
      601 catcgcactc ctgacgcggc gccattaccg gaacacgttc aagctcctgc accgcctcga
      661 cgaactcgtg aatgcggccg aggcctcgtg ccccatcaac ccggaatcgc cggagggtca
      721 gatgctcaag gtcaagtacg atccccgcga taagacggcg gtcctgctcg taaacgggtt
      781 caacggtctg ggcctgcaca cgctgttcag catcatccgc ctcttcggcg ggacgttcaa
      841 aaacttcgtg ttcattcagg tgggggtcgt ggacgccggc aacttcaagg gtgccgagga
      901 agtcgcgcgg atgaaggagg aagtgaagaa agagcttgac cgatacgtgc attacatgcg
      961 gtgccacgga tactacgcct cggcctactc cggcttcggg acggacgtgg tcgatgaggt
     1021 ggcaaatctt acgccccaga tcctggaacg gttcccgaac gccattctct tcggcggcca
     1081 gctcgtcttc cccaggacca cgttcctgtc cggcgtattt cacaactaca ccatttttgc
     1141 cgttcagcgg cgtttctaca accaggggat tcccgtcgtg atactcccga tccgtgtttg
     1201 acggcgctgc gggaataagt tcgggaggga aaagaaaaag cgcggagagg tcttgagaat
     1261 cggacaatgc tgaatgaggg aaacggcgtg gcgcctgccg cttcccccac agagcagtat
     1321 tatttttttg ccatccccgc ccggaaggta tcgagccagc ggatgtggct cttttcctcg
     1381 ttgatgacct cgtcgatgac ctccttctcc ttcacgagcg tcctcagctc gatgaagtag
     1441 agcagcgtct ccttttcgaa tccgatggcg aatttaacgg cgtcatccac ggtcctgatg
     1501 tggtccatgg agggaagaga cttccctcgg ccgaggaaaa actccgattc gatgaaggcc
     1561 cgcatgtaat tgctcacctc ttcccactgg accggctcgg ttccctggcg ggggaccgag
     1621 tccttgaggt cggaaaaggt cttctcgtga gacgtttcct tatctgccag cgtggtgaag
     1681 agcttgacca atccgccgtc cttcttgaat ttctccgcca tcgccaggta gaactgccgc
     1741 ccgagcactt ccgtctgtac cgccatttcc atgatttcgc cgatcgaata cctgttcatc
     1801 gtggtctccc ctgtttcgcc tgaatagaat aattccgcgg aaaaggtccg cggataatga
     1861 catacaaaaa taatagcgtg aataccggac caagtcaacc ctttaccatt ccggaagccg
     1921 cacggctcga aaagacacac gattctaaaa aacaattttg gacgcaggta aaatccgata
     1981 aacgcggatg aagcaagaat aaaaattggt gcaagccttt acctcacgcc cgg
//
LOCUS       DASSLD010000039         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1234084, whole genome shotgun sequence.
ACCESSION   DASSLD010000039 DASSLD010000000
VERSION     DASSLD010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234084"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VFK23_00715"
     CDS             <1..622
                     /locus_tag="VFK23_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HET7317634.1"
                     /translation="RSGGALGRGCGPLIVAKPGVCLADLKKGAIAIPGKLTTAHLLLR
                     LFDPSLTTVLVMPFDRIMDAVSRGEAIAGLIIHESRFTYPRYKLEKLLDLGEWWERHA
                     GLPIPLGGILGKRSLGRETLIKVESGIRESLRFARTHPDEVLRYCRQHSQEMDEIVMK
                     NHIDLYVNDFSLDLGQEGMAAIKKLFDEAESRGIFPHSGKPLLAGE"
     gene            716..1276
                     /locus_tag="VFK23_00720"
     CDS             716..1276
                     /locus_tag="VFK23_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET7317635.1"
                     /translation="MIGWIFLAVLGLMLVWIVLIYNSLVSIRNDVQSSWKQIDVQLKR
                     RHDLIPNLVQTVKGAMEFEQDTLEKVISARAKAVSAATPGTKAEAENMLTQALGKLFA
                     VMENYPALKSNQNVMQLQEELTSTENRIAFARQLYNDLVANLRTKLQVFPNNIVSSLF
                     NFQPEEYFGAEETDRAAPAVNLSLRK"
     gene            1454..>2053
                     /locus_tag="VFK23_00725"
     CDS             1454..>2053
                     /locus_tag="VFK23_00725"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET7317636.1"
                     /translation="MLMYLVLCLALAAVFVPHALFFSKTFATGRLMTAGFLVAFLVAG
                     IHFLFAGYDAVNSMIRNLDAQRPDPGDTVHKTLLNIMQEIQLVTGNRRNIRCVVIPSL
                     SLNALAAADLKGESVIGITEGLLSRLSRPQLETVIAHEAHHILSGDCLETTVAASLFG
                     SLSSLVDRYLQTNRGGQFPPPALWPAWGLLKLSYLLNMFI"
BASE COUNT          438 a          620 c          555 g          440 t
ORIGIN      
        1 ccgctccggc ggagcgctcg gccggggctg cggtccgctc atcgtcgcca agccgggcgt
       61 ctgcctcgct gatctgaaaa aaggcgccat cgcgatcccc ggaaaactta cgaccgccca
      121 tctactcctt cggctcttcg acccctcgct cacgaccgtt ctggtcatgc ccttcgaccg
      181 catcatggat gccgtttccc ggggagaagc gattgccggc ctcatcatcc acgaaagccg
      241 tttcacctat ccgcgctata agcttgaaaa gctgcttgac ctcggtgaat ggtgggagag
      301 gcatgcaggt cttcccatcc ctcttggcgg cattctgggg aagcgctccc tgggcaggga
      361 aacgctgatc aaggtcgaat ccggcatccg ggagagtctg cgcttcgccc ggacgcaccc
      421 tgacgaagtc ctccgatact gcagacagca cagccaggaa atggacgaaa tcgtcatgaa
      481 aaaccatatc gacctgtacg taaatgactt ttcactggat ctggggcagg aagggatggc
      541 ggcgatcaag aaactctttg acgaagcgga atcacgcggc atctttcccc attccggcaa
      601 accgctcctt gcgggagagt gaacactttc tcatcccgct gaacggaaac catgttataa
      661 tgctggtgaa ttcggcatcg actgtcatga aatgaactct cttggggggg ggaatatgat
      721 cggatggatt ttcctcgcgg tgctcggctt gatgttggta tggatcgttc ttatctacaa
      781 cagcctcgtg tctattcgga acgatgtgca gagttcctgg aagcagattg acgtgcagct
      841 caagcgacga cacgacctta tcccgaattt ggtccaaacg gttaagggcg ccatggagtt
      901 cgagcaagac accctcgaaa aggtgatctc ggcccgggcc aaggccgtct cggctgcaac
      961 tccggggacg aaggccgagg ctgagaatat gcttactcag gctcttggga agctgttcgc
     1021 cgtgatggag aactatcccg ccctcaagag caaccagaac gtaatgcagc tgcaggagga
     1081 gctcacgtcc accgagaacc gcatcgcctt tgcacgccag ctctacaacg acctggtggc
     1141 caacttgcgt acaaaactcc aggtctttcc caacaatatc gtctcatccc tgttcaactt
     1201 ccagcctgag gaatacttcg gcgccgagga aacggaccgt gcggcgccgg cggtgaacct
     1261 gagcctgagg aagtagagcc cggagattac gagggacgaa ggacgaccgc gatgcttgga
     1321 cgacggacga agattctaat cgtccgtcgt caatcgccca tcgccccgca ctgccttgct
     1381 cgccatgccc ttgacgttca tcgacataga gagacaaaaa agctggagga tctgggcttt
     1441 tttccttatc ctcatgctga tgtaccttgt cctctgtctc gcacttgccg cggtgttcgt
     1501 cccgcatgcc ctattcttct ccaaaacctt tgccaccggg cgcctcatga ccgcgggttt
     1561 cctcgttgct tttctggttg cgggcattca cttcctcttc gcaggatacg acgcggtaaa
     1621 cagcatgata agaaatcttg acgctcagcg gccggacccc ggcgatacgg tgcataaaac
     1681 cctcctgaat atcatgcagg agatccagct cgtcaccggc aacagacgga acatccgctg
     1741 cgtcgtgatc ccgtcgcttt cgctgaatgc gctggccgca gcagacctga agggcgagtc
     1801 cgtaatcggc atcaccgaag gtctgctctc gcgcctgagc cgtccccagc ttgagaccgt
     1861 gatcgcccac gaagcgcatc acattctctc gggggattgc ctggaaacaa ccgtcgcagc
     1921 gagcttattc ggatctctct catcgctcgt cgaccggtat ctccaaacga atcggggagg
     1981 acagttccct cctcccgcgc tctggcccgc gtggggtttg ctgaaactga gttacctgct
     2041 caacatgttc atc
//
LOCUS       DASSLD010000040         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_451063,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000040 DASSLD010000000
VERSION     DASSLD010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451063"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1660
                     /locus_tag="VFK23_00730"
     CDS             <1..1660
                     /locus_tag="VFK23_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7317637.1"
                     /translation="GGINVPEYTNEELYERGRNLSLLGQHDRAVEIYQKLIASELSAP
                     NKPELYLRTGVALYNLRKRDAAINILEKQIHEYPSDPRVPEALYWTGRAYSKNGDGER
                     AVKTFRQLLDRFPGSDCCGDALYFTGNIHRDANDMKKAIQAYRRLARDYPESRFADSA
                     LWWVAWSFFSSGEYRKAEQALQDLINRYPRSSLLNQARYWQGRAAEKSDDPSRASAYY
                     SRLVKKAPYTYYGYRASERLARLKTGATVLPAEMSLSEPADCLAGPCPGGPSAPPENE
                     VGPPVWTDEAKKVLSADPSYKKTLELMSLDMRKEAAQELSSLQDRVSRKSGMLLGLSK
                     TFFELGDYYHSLLLVIRNYENYLESPDNGASDDVWLLAYPQGYWESVLSYSRKYGQDP
                     YFIAAIIRAESQFSPDALSPVGARGVMQVMPATGEKVARLIPLPGFDPTRLFEPDTAI
                     NIGAWYIGSLMKRFKGDPILVAAAYNAGPEAVQAWISKNGYHGEGDVFVESIPYYETR
                     GYVKKVLRNYAEYRRIYGKASAVASGPARDMTSMGSNGRPAENP"
     gene            1657..1941
                     /locus_tag="VFK23_00735"
     CDS             1657..1941
                     /locus_tag="VFK23_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317638.1"
                     /translation="MKGRQDPTMRQGEKSNPAGKTLEERFAIVEQRIRALLDENGTLA
                     VRVRELERELAQARNTALNIEEYRGKAAHIRRKVETVLQSLESLNAKKED"
     gene            2128..2862
                     /locus_tag="VFK23_00740"
     CDS             2128..2862
                     /locus_tag="VFK23_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7317639.1"
                     /translation="MDIIIAQNLIKDYDGLRAINGINFSIGQGECFGFLGPNGAGKTT
                     VMRTISCFMPPTSGTVTVFGMDVTTRPSEIKARIGVMPQEINLDPDLSVLENLIVYAR
                     YFDIRKKDSVPVARGLLDFVELAEKGGVQIDELSGGMKHRLLLARALINNPEILILDE
                     PTTGLDPHSRRAVWDKLDHLKFQNTTLLLTTHYMEEAERLCDRVAIMDSGRIVTIDSP
                     SNLMAIHGGGNLEDVYLKLTGRSLAD"
     gene            2852..>3033
                     /locus_tag="VFK23_00745"
     CDS             2852..>3033
                     /locus_tag="VFK23_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7317640.1"
                     /translation="MQIKRAFRVWQRQWLVYTKLYKTSFALNFAEPALYLVAMGIGLG
                     SFVQDIHGQPYIKFIAP"
BASE COUNT          700 a          906 c          879 g          548 t
ORIGIN      
        1 gggaggcatc aatgttcccg aatacacgaa tgaggaactc tacgagcggg gcagaaacct
       61 ctccctcctg ggccagcatg acagggccgt tgaaatttac cagaaactta tcgcgagcga
      121 gctgtccgct ccaaacaagc cggaacttta tttacggacc ggcgtcgccc tgtataacct
      181 gcgcaagcgg gatgcagcga ttaacatcct ggaaaagcag attcacgaat atccctcaga
      241 cccgcgcgtt ccggaggcgc tgtactggac cggaagggct tacagcaaga atggcgacgg
      301 ggagcgggct gtgaagacgt tccggcagct cctcgaccgc tttcccggca gcgactgctg
      361 cggcgacgca ctctatttca ccggaaacat ccaccgcgac gccaatgaca tgaagaaagc
      421 gatccaggct tacagaaggc ttgcgcggga ttatccggaa agcaggttcg cggacagcgc
      481 tctgtggtgg gtcgcctggt ccttcttctc ctccggcgag taccggaagg cggagcaggc
      541 gctccaggac cttatcaacc gatacccccg ttccagcctc ctgaaccagg cgcgctactg
      601 gcagggaagg gcggctgaaa aaagcgacga tccgtcacgg gcgtcagcgt attacagccg
      661 cctggtgaaa aaagccccct atacctacta cgggtaccga gcaagcgaac gactcgcccg
      721 cctgaagacc ggagcgactg tccttccggc ggaaatgtcc ctcagcgaac ctgcggattg
      781 cttggcgggg ccgtgcccgg gcggtccgtc agcccctccg gagaatgaag tggggccgcc
      841 ggtctggacc gacgaggcaa aaaaggtcct ctctgcggat ccgtcctaca aaaaaacgct
      901 cgaactgatg agcctggaca tgaggaagga ggcggcgcag gaattgtcgt ccctccagga
      961 cagggtttcg agaaaaagcg gaatgctcct cggcctgagc aagacattct tcgaactcgg
     1021 tgattattac cactcactcc ttctcgtgat cagaaactac gagaactatc tcgaaagtcc
     1081 ggacaacggc gcatcagacg atgtgtggct ccttgcctat ccccagggct actgggagag
     1141 cgtcctgtcc tactcccgga aatacggcca ggacccctat ttcatcgccg caatcatccg
     1201 tgcggagagt cagttttccc ccgatgcatt atcgccggtc ggagcgcggg gtgtcatgca
     1261 ggtcatgccc gcaacagggg agaaggtagc acggctcatc ccgctcccgg gatttgatcc
     1321 cactcggctc ttcgaacctg atacggccat caacatcggc gcctggtaca tcggcagcct
     1381 gatgaagcgg ttcaagggcg atcccatcct cgtcgccgcg gcatacaacg cgggccccga
     1441 ggccgtgcag gcgtggatca gtaaaaacgg ataccacggc gaaggggatg tgttcgttga
     1501 gtcgatcccc tattacgaaa cccgcggtta cgtgaagaag gtgctcagga attacgccga
     1561 ataccggagg atctacggca aggcatctgc ggtcgcatcc ggtccggcca gagacatgac
     1621 cagcatgggc tcgaacggcc gtcccgcgga gaacccatga agggacgaca ggatccgaca
     1681 atgaggcagg gagagaaaag caatccggcc gggaagacgc tagaggaaag gtttgcgatc
     1741 gtagaacagc gtatccgggc tctcctggac gagaatggca ccctcgcggt ccgcgttcgc
     1801 gaactggaaa gggaactggc gcaggcacgg aacactgcct tgaacatcga agagtaccgg
     1861 ggaaaagcgg cccatatccg ccggaaggtc gagaccgtcc tgcaatccct cgagtcgctc
     1921 aacgcgaaga aagaggattg agcgcagcta tcttatttca ccgatcagca ggagcaagag
     1981 gcgtctcgcg caaaggcgca aaggacgcaa aggaaacgat gaagtcgatc tttaaagtct
     2041 aaaaaacttt cgactttctt tgcgagcttg gcggctttgc gcgcaaattt ctttacgaga
     2101 gactttgatt cgtttcccga aaccccgatg gacatcatca ttgcgcaaaa cctcatcaag
     2161 gactatgacg gccttcgggc gatcaacggg ataaacttct ccatcggaca gggggagtgc
     2221 ttcggctttc tcgggccgaa cggcgcgggc aagacgacgg tcatgcggac catctcctgc
     2281 ttcatgcctc ccacctcggg gacggtcacg gtcttcggca tggatgtgac gacgcgtccc
     2341 agcgaaatca aggcacgcat cggcgtcatg cctcaggaaa tcaacctgga ccccgacctc
     2401 tcggtcctcg agaacctgat cgtctatgcc cgatattttg atatccggaa aaaagactca
     2461 gtccccgttg cccgcgggct tctcgatttc gtggaactcg cggaaaaggg cggcgtccag
     2521 atcgacgagc tttccggagg catgaagcac cgcttgctcc tggcccgtgc gctcatcaac
     2581 aaccccgaaa tactcatcct cgacgagccc acgacaggcc tggacccgca cagcagacgg
     2641 gcggtctggg acaagctgga ccatctcaag ttccagaaca ccacgctcct tctcacgacc
     2701 cactacatgg aggaggccga gcggctctgc gaccgggtcg cgatcatgga ctcgggcagg
     2761 atcgtgacga tcgattcgcc gtcgaacctc atggccatcc acggaggcgg caacctcgaa
     2821 gacgtgtatc tcaaactgac ggggaggagc cttgcagatt aagcgcgcct tcagggtttg
     2881 gcagcgtcag tggctggtct acaccaagct ctacaagacg agcttcgcgc tcaattttgc
     2941 ggagccggcc ctctatctcg tcgccatggg gatcggcctc ggctcctttg tccaggacat
     3001 ccacggccag ccctatatca agttcatcgc gcc
//
LOCUS       DASSLD010000041         3474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1560241, whole genome shotgun sequence.
ACCESSION   DASSLD010000041 DASSLD010000000
VERSION     DASSLD010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560241"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VFK23_00750"
     CDS             <1..763
                     /locus_tag="VFK23_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317641.1"
                     /translation="MHEVTEATKKSADAAEAAAKISKNSFDFNKDALLVEIQKQTRAF
                     QASADSMKETVKQGNAALNDNIKNFRVEHRAWVGTMQAVTAAYMEGGKNRYVKEGQPA
                     KCGVILINSGRTPARKLRGVVSLLTLNAGEELAPKFEIDNTTIPHSNVVMQPGTQLML
                     LPSLNTLNVKLPGILVETSDASMAGTGEASVKEKPYSKADIDDLASGKKVLYMFGLIT
                     YEDIFNQSHWTKFCMYLSTDLSTFTGCPQYNETDN"
     gene            complement(871..2037)
                     /gene="comE"
                     /locus_tag="VFK23_00755"
     CDS             complement(871..2037)
                     /gene="comE"
                     /locus_tag="VFK23_00755"
                     /EC_number="4.1.1.79"
                     /inference="COORDINATES: protein motif:HMM:TIGR03846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfopyruvate decarboxylase subunit beta"
                     /protein_id="HET7317642.1"
                     /translation="MPRPEEDLITLLKKHRIDFTASLPCEKIKTLLEMVGRDFPHLPL
                     TREEEGVGICAGAALAGKRPAVFVQSSGIGNMINALLSLTGFYQLPLALFVSRRGIYK
                     EKIEAQMPMGERLPSILAGSGIMYSKINSKNDIGTIDRELQEIFNRNTIHAFLMSPQV
                     WEGSECGMQNVDAEKRGRGDADLQSISSPCPPVTPPPRLPLPRATLTRYDIIRIIAPY
                     LEDKAVVCNLGWPSKELYSLKHQPSNFYMLGSMGMATPVGLGIALTSSKEIVVIDGDG
                     SLLMNPGTLATAASVAPKNLTIIAIDNGAYGSTGNQPTLASSCVDLEQVARGFGFRET
                     VKVSTEKEIIGRMKQQRQGPSFIHVLAIPGNRDVPNIPVHHLEIKKQVEEFLKK"
     gene            complement(2010..3272)
                     /locus_tag="VFK23_00760"
     CDS             complement(2010..3272)
                     /locus_tag="VFK23_00760"
                     /EC_number="2.5.1.76"
                     /inference="COORDINATES: protein motif:HMM:TIGR03844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteate synthase"
                     /protein_id="HET7317643.1"
                     /translation="MSHFSIKCRACGKTLENTFCAFCEHCKDALLVTDYRNAPFTESA
                     TSSGIWRFNWLPVHEPKVVQPGPIVYQSHGLAGRFGLENLFIAFNGYWPEKGALLETC
                     TFKEFEAAVVLQNARENGVEGLIVASAGNTARAFAHLSAVTGFPVVIVVPRMCLMEMW
                     YLEQKSTVPTLAVGDGDYADSIDVAKRIALAAGFPFEGGVKNIAKRDGLGIVMLEAVS
                     KIGRLPDHYIQAVGSGTGAIGGWEMAERFVRDGRYGTRLPVLHLGQNLPFAPMLHAWE
                     KNNRHLDPDDLRPELIGFITTRVLSTRYPAYSVKGGIYDALLATDGKMYGIENDAVYA
                     GMKAFLETEGIDVVPASGVAVAVLQEAVQNGAIKKDEAVLLNITGGGEARLRREKETH
                     AVIPQFISKYATEKQIEELLCHARKKTS"
BASE COUNT          756 a          955 c          968 g          795 t
ORIGIN      
        1 gatgcatgaa gttaccgaag ccacaaaaaa gagcgctgat gctgccgaag ctgccgcaaa
       61 aatctcaaaa aattccttcg actttaataa ggatgccttg ctcgtagaaa tccagaaaca
      121 gacaagggct tttcaggcca gcgctgattc catgaaagaa acggtgaagc aaggcaatgc
      181 tgccctgaat gataacatta aaaactttag agtggagcat cgagcctggg taggcaccat
      241 gcaagctgtc accgctgcct acatggaggg aggtaaaaat cgttacgtca aggaagggca
      301 accggcgaaa tgcggcgtaa ttttaatcaa tagcggcagg actccggccc gcaaactccg
      361 tggtgttgtt tcgttgctta ctcttaacgc tggggaagaa cttgctccaa agtttgagat
      421 cgacaatacg acgattccac atagtaatgt cgtgatgcag ccagggacgc aactgatgct
      481 cttgccatct cttaatactt taaacgtcaa actccccggt attctggtag agacttcaga
      541 tgcctcgatg gctggcaccg gtgaggcgtc tgttaaagag aaaccttatt ctaaagccga
      601 cattgatgac cttgccagcg gtaaaaaagt cctctatatg ttcggactca tcacgtatga
      661 ggatattttc aatcaatccc attggacaaa attctgcatg tacctcagta ccgatctatc
      721 cacattcacc ggctgccctc aatataacga aactgataat tagaaacggg cagacaaaca
      781 taacgaacgc atccactccg acgccgaata agcgctttct gttttcagtc gtttgtcccg
      841 gtgggcgcgg cagatgcatt atgttggccg ctacttcttc aaaaattcct cgacctgctt
      901 cttgatttcc agatgatgga ccggaatatt cggcacgtcc ctgttgccgg gaattgccag
      961 gacgtggata aaggagggac cctggcgctg ctgcttcatt cttccgatta tttccttttc
     1021 cgttgaaacc ttcaccgtct cccgaaaccc gaaaccccgt gccacctgtt cgagatcaac
     1081 gcatgacgag gccagcgtgg gctggtttcc cgtcgaaccg taggccccgt tgtcgattgc
     1141 gatgatcgtg agatttttcg gtgcaacgga tgcagcagtt gcgagcgtac cggggttcat
     1201 gagcaggctt ccgtcgccgt cgatcacgac gatttccttt gatgaggtca gggcgatccc
     1261 aagcccgacg ggcgttgcca tgcccatgct gccgagcatg tagaagttcg agggctggtg
     1321 cttgagtgag tacagctcct tggagggcca tccgaggttg cagaccacag ccttgtcttc
     1381 caggtacggc gcgatgatcc tgatgatgtc gtagcgggtc aacgttgcac gcgggagagg
     1441 cagacggggg ggcggggtga cggggggaca gggggatgaa atgctctgaa gatcggcgtc
     1501 tccgcgtccc cgtttctccg cgtcgacatt ctgcatcccg cactcagacc cttcccaaac
     1561 ttgcgggctc atcaggaagg catgtattgt attcctgttg aagatctcct gaagttcccg
     1621 gtctattgtg ccgatgtcgt ttttactgtt tatttttgaa tacatgatcc cggagccggc
     1681 caggattgac ggaagccgtt cgcccatcgg catctgggcc tcgatcttct ccttatagat
     1741 accccgcctg cttacaaaaa gtgcgagagg aagttgatag aagccggtca gggagagcag
     1801 ggcattgatc atgttcccga tgccggagct ctggacgaac acggccggcc gtttgcccgc
     1861 gagggcggcc ccggcgcaga tgccgacgcc ttcttcctcc ctggtgagcg gcaggtgcgg
     1921 gaagtcccgg ccgaccattt cgaggagggt cttgatcttc tcgcaaggca gggaggcggt
     1981 gaagtcgatc cggtgcttct tgagcagggt tatgaggtct tcttccgggc gtggcacaag
     2041 agctcctcga tttgtttttc ggtggcatac tttgatatga actgaggtat gacggcatgg
     2101 gtctcttttt ccctcctcag ccgagcttcg ccgccaccgg tgatgttcag gaggaccgct
     2161 tcgtcctttt ttattgcgcc attctgcacg gcctcctgaa gcacggcaac ggccacgccc
     2221 gaggcgggca cgacatcgat cccctctgtt tcgagaaatg ctttcatgcc ggcatagacc
     2281 gcgtcatttt ctatgccgta catcttcccg tccgtcgcga gaagcgcatc gtatatgcca
     2341 cccttgaccg agtaggcggg atagcgggtg gagaggaccc gcgtcgttat gaacccgatc
     2401 agctcgggtc tcagatcatc cggatccaga tgccggttgt ttttttccca ggcatggagc
     2461 atcggcgcga agggcaggtt ctgtcccaga tgaagtacgg gcagccgcgt tccgtaccgt
     2521 ccgtccctga cgaaccgctc ggccatctcc caaccgccga tagcgcctgt gccgctgcct
     2581 acggcctgga tgtaatggtc cggaagccgg ccgatcttcg acacggcctc gagcatgacg
     2641 atgccgaggc cgtcgcgttt tgctatgttc ttgacgccgc cctcgaaggg aaagccggcg
     2701 gccagggcga tccttttcgc gacatcgatg gagtcggcgt aatcgccgtc tcctacggcg
     2761 agcgtcggca ccgtggattt ttgctcaagg taccacatct ccatgaggca catgcggggc
     2821 acgacgatga cgaccggaaa gcccgtaacc gccgaaagat gggcaaaggc gcgggcggtg
     2881 tttcccgccg aggcgacgat caagccctcg acaccgttct cccgggcatt ctgaagcacg
     2941 accgcggcct cgaactcctt gaaggtgcac gtttcaagaa gcgccccctt ctccggccag
     3001 tacccgttga aggcgatgaa gagattctca agcccgaacc gccccgcaag gccgtgcgac
     3061 tggtacacga tgggtccggg ctgcacaacc ttcggttcat ggaccgggag ccagttgaac
     3121 ctccagattc cgcttgacgt cgcgctttcg gtaaagggag cattgcgata gtccgtgacc
     3181 aggagcgcgt ccttacagtg ctcacagaag gcgcagaagg tgttctcaag ggttttcccg
     3241 caggcgcggc attttatgga aaagtggctc attgctgatt acctttcaac aacacaaagg
     3301 cttcttggac acgggaaaat cgggaaaaac ggaaaaagta gggtacgccg aatgaattga
     3361 tgctatgaac gcgttatctg cagtatccaa gtttaccgcc ttttccgtgt ccaaaggctt
     3421 tcattcgatc agtccgctca ccagctccat gatgtccgtg atccgtatct ggtt
//
LOCUS       DASSLD010000042         3573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_679728,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000042 DASSLD010000000
VERSION     DASSLD010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679728"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1582)
                     /locus_tag="VFK23_00765"
     CDS             complement(<1..1582)
                     /locus_tag="VFK23_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7317644.1"
                     /translation="MENTSYDVIVIGGGINGLTAGAYLAKAGAKVLITERRWETGGAV
                     MTEDVSGCRINTHATYMMMMDVAPPYADLELEKWGCTYITPQPAVSILLKNGRSLSLY
                     NDVEKSVASIMKFSAKDAGIYRKVYSEFKEMCDECLVPQTYRPAMPPLELLTMLNDKP
                     LGQKILEISEKSPREIIEECGFETEALKALLLYLTSMWGINPNTTGVGYLVPLYICRM
                     LTSSLIRGGSHRLPSAILKSYIANGGEVIETIDVENVIIENRKAVGIRTTKGLEFRAK
                     AILSTVDPEQSFLRFIGDKQLDSLSPGMADAAKGWQWEEVSHFSLHLVAKSEPVFHAE
                     RADPDCGQALVQVLGVETLEEVVAKYNLAKEGGLPHWGHVTTTTRHDRSQSPTVVEKF
                     LPEWQAAGIRMHVVRLEAIVPYSLKNGDWEKEKDRYSVGMRNLLNEYAPNMANAVTVR
                     NFTYPPTYIEMKFPNMKKGSIKHGEYQSTQMGYFRPNDLCSQYKTPIDGYYLGGASVF
                     PGGMVLLGSGYGAANAIAK"
     gene            complement(1621..3342)
                     /locus_tag="VFK23_00770"
     CDS             complement(1621..3342)
                     /locus_tag="VFK23_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7317645.1"
                     /translation="MKDSYDVVVVGGGLQGLASAAYLAKCGLSVAVFEARNEVGTHCD
                     TEEVMRPGVKCNLQATGLVPWVSPAYEHLELERFGYEPKMGGWGFMYSFQDGTALCIH
                     NYDTEKTYNAWKMLSPKDAETFRDICNYFGKNDRLVNFMNELFYRKPSPASFRKALQI
                     LGGCPHMPPGWTEMTGYHLVDLMFTEERIKVGLLSLTVSMGADPWLKTIGPLGVIMAM
                     FTGPAVSMLSTAKGGSHLLTHTVYRALIHYGGEVFQSCPVDKIIVENGAAKGVVLSEH
                     SVYPGRKIMGSKAVISDLTVVPTFLWMVGAEHLPPEVAMAVQNYDYEAQVLFTNYYVL
                     SEPLRFKSFDWTNKHVDPMIEKEIYMNTFSVETEIDTARLQRCIVMGELPDPPIAFSG
                     CFRPTSLDPTQAPPGMHTLLVWTDVPYNIRRWGNRKLNGPDDWDLIREEYADRVEDKL
                     AEYAPNLKTAKVERYVHTPLDITRRNQSMLMGTWSGGGLNNNQWGANRPFAGCGSPRT
                     PVKNLYITEVNIARASWMVGAYVCADTIAEDLGVRNQPWWTGTRAMEPYIDYCRRNGL
                     NFKVGGF"
BASE COUNT          702 a         1122 c          940 g          809 t
ORIGIN      
        1 ccttcgcgat cgcgtttgca gcaccatacc cggatcccag caagaccatt cccccgggaa
       61 atacgcttgc cccgccgaga taatatccat cgatgggtgt cttatactgg gaacacaggt
      121 cgttcggcct gaaatatccc atctgggtac tctggtattc cccgtgcttg atggagcctt
      181 ttttcatgtt gggaaatttc atctcgatat atgtcggcgg ataggtgaaa ttgcggacag
      241 tgaccgcatt cgccatgttc ggtgcgtatt cattgagcag atttctcatg ccgacggagt
      301 aacgatcctt ctccttctcc caatctccat tcttcaggga atacggaacg atcgcttcca
      361 ggcgaaccac gtgcatgcgg attccggccg cctgccactc ggggaggaat ttctcaacga
      421 ccgtcgggga ctgggacctg tcatgcctgg tcgtcgtcgt cacatgcccc caatgcggga
      481 ggcctccctc tttcgcgagg ttgtacttgg cgacgacctc ttcgagggtc tctacgccca
      541 ggacctggac cagggcctgc ccgcaatccg gatcggcacg ttccgcgtgg aagaccggct
      601 ccgatttcgc caccaggtga aggctgaaat ggctgacctc ctcccattgc cacccctttg
      661 ccgcatcggc cattcccggg gacaggctgt ccaattgctt gtcaccgatg aaacgcagga
      721 acgactgctc cgggtcgacc gtactcaaga tggctttcgc ccggaattca aggcccttcg
      781 tcgtccgtat cccgacggcc ttcctgtttt cgatgatgac gttttccacg tcgatcgttt
      841 cgatgacctc gccgccgttg gcgatatagg acttgaggat cgcggaaggc aaccggtggc
      901 ttccgccgcg aatcaatgaa gaggtcagca tgcggcagat atacaaggga accaggtacc
      961 cgacaccggt agtattggga ttgattcccc acatgctcgt caggtagagg agaagcgctt
     1021 tcaacgcttc cgtctcgaag ccgcactcct cgatgatttc gcgcggggat ttttccgaga
     1081 tctccaggat cttctgtccc aacggcttat cgttcaacat ggtcagcaat tcgagcggag
     1141 gcatcgccgg ccggtacgtc tgcgggacaa ggcattcgtc gcacatttcc ttgaattccg
     1201 aatacacctt cctgtagatc ccggcgtctt tcgcggaaaa tttcatgatg gaagcgacac
     1261 tcttctccac atcgttataa aggctcaggg atctcccgtt cttcaggagg atggacaccg
     1321 caggttgcgg ggtgatgtac gtgcatcccc acttttccag ttccagatct gcgtatgggg
     1381 gggcgacatc catcatcatc atatacgtgg catgcgtgtt tattcggcag cccgacacat
     1441 cctcggtcat cacggcgccc cccgtctccc atcggcgctc cgtgatgaga accttcgcac
     1501 cggccttcgc gagatatgct cccgccgtca ggccatttat tcctcccccg atcacgatca
     1561 catcgtagga tgtattttcc acgctccctg cctccccttt cgtgatggcc ctggtccgaa
     1621 ctaaaatccc cccaccttga aattcaatcc gttcctcctg caataatcga tatacggctc
     1681 cattgcccgc gtcccggtcc accatggctg gttccgcacc ccgaggtcct ccgctatggt
     1741 gtccgcgcaa acatatgcgc ccaccatcca cgatgccctc gcgatgttca cttccgtgat
     1801 gtacaagttc tttaccggcg ttcgcggaga cccgcaaccg gcgaacggac ggttggcgcc
     1861 ccattgattg ttgttgagcc cgcctccgct ccatgtgccc atcagcatgc tctggttccg
     1921 cctcgtgata tccagcggag tgtgcacgta ccgctccact ttcgccgtct tgagattcgg
     1981 cgcgtactcg gccagcttgt cctcgacccg gtccgcgtac tcctcccgga tcagatccca
     2041 gtcgtccggg ccgttcaact tcctgttccc ccaccgccgg atgttgtacg ggacatccgt
     2101 ccagacgagg agcgtgtgca tcccgggagg agcctgtgtg ggatcgagcg acgtggggcg
     2161 gaaacaaccg ctgaacgcga tggggggatc cggcaactct cccatgacga tgcaccgttg
     2221 cagccgcgcc gtgtcgattt ccgtctcgac gctgaaggtg ttcatgtaaa tttccttctc
     2281 gatcatgggg tcgacatgct tgttggtcca atcgaaggat ttgaaacgaa ggggttcgct
     2341 caagacgtaa tagttggtga acaatacctg ggcctcgtaa tcgtagttct gcacggccat
     2401 cgcgacctcc ggcggcaggt gctccgcccc gaccatccag aggaacgtgg gcacgacggt
     2461 caggtcgctg atgaccgcct tgctccccat gatttttcgg ccgggataga cggaatgctc
     2521 ggacaggaca acccccttgg ccgccccgtt ctcgacgatg atcttgtcca cggggcaact
     2581 ctgaaaaact tcccctccgt agtgaatgag cgcccgatac accgtgtggg tcagcagatg
     2641 gcttcctccc ttcgccgtgc tcagcatgga gaccgccggg cccgtgaaca tcgccatgat
     2701 cacaccaagg gggccgatcg ttttcaacca ggggtcggct cccatcgaca ccgtgagaga
     2761 gagcaatcca accttgatcc gctcctcggt gaacatcagg tcgaccaggt ggtaccccgt
     2821 catctccgtc cagccgggcg gcatgtgagg gcatcccccc aggatctgga gggccttcct
     2881 gaaagacgcc ggactcggtt tccggtagaa caattcattc atgaaattca cgaggcgatc
     2941 gttcttgccg aagtagttgc agatgtcccg gaacgtctcc gcgtctttcg gactgagcat
     3001 cttccacgcg ttataggtct tctcggtgtc gtagttatgg atgcaaagcg ccgttccgtc
     3061 ctggaacgag tacatgaacc cccagccgcc catcttcggt tcgtaaccga accgttccag
     3121 ctccaggtgt tcatacgcag gggaaaccca gggaaccagc cccgtggcct ggaggttaca
     3181 cttgacgccc ggcctcatga cctcttcggt atcgcagtgc gttccgacct cgtttcgcgc
     3241 ctcgaagacc gcgacgctga ggccgcattt cgccaggtag gcggcgcttg ccagtccttg
     3301 aagaccccca ccgaccacga ccacatcata ggaatctttc atacggcctc catcgattca
     3361 taatgtgcaa gcaaggagtc cccaggttct cacgcgaact tcaaggctat cgtatttccc
     3421 agatgactcg aggccaacga caaaccatat tcttttcgga gaaaggcagc aatatatatg
     3481 ccacgtcatc gacaagggaa cgacgcacgc ccctcattac cgtgtcccgt gtggtgaacc
     3541 ccatgatgtc attgaggaaa acaggccagt cac
//
LOCUS       DASSLD010000043         5203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1070114, whole genome shotgun sequence.
ACCESSION   DASSLD010000043 DASSLD010000000
VERSION     DASSLD010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070114"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="VFK23_00775"
     CDS             <1..1381
                     /locus_tag="VFK23_00775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7317646.1"
                     /translation="AGEAVMVRDYIGQIKAILAQHPRFGEEDQRALGMGIAAYQGRFD
                     RIDSSAAELTAVFEDLKSRDGKNATYLSLVEETFLDRPGQSAYFLTKVFRMPEHHRLV
                     VGLRRLDADIAALRKNGEDILVTTKDLDKAAREKAETVIRVSQLAILIFFPLFFAVGI
                     GMLFFITANVVRRLMLLITVVEKTGKGSYPHLTVAPSDGRARDEVGVLIEKFNDREDQ
                     LALREQELGRKNQELMQAKKLAAIGTLASGVAHELNNPLNNIYLSAQALTRDAGESCS
                     PEVKEAVGDIVGQTERVKRIVSDLLEFARGREPQYQDVDLCGLITDAYRHAASSRDLS
                     GIRYACSSDPFRIIASLDQGQMEQVFTNLFLNAADAMQAKGEITVTAKREAQDVVIRV
                     SDSGRGIPRESQDKIFEPFFTTKDKGTGLGLAIVYNIIKKHDGDITVQSEEGKGTTFV
                     ITLPVGKTP"
     gene            1522..2853
                     /locus_tag="VFK23_00780"
     CDS             1522..2853
                     /locus_tag="VFK23_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7317647.1"
                     /translation="MAFKILIAEDEEITLKHLINALQKAGYTAVGTQNGQDAFQKIEN
                     DHFDLLIADIKMPGMTGMELLDKIKERQIEAEVIIITGFGSIGSVVEAMKKGAYDYVT
                     KPFNLDELLLKVGKIREQRTLKKENIALKAFMGMDRKVSIIAKSASMNRILDTIDGMK
                     DSDCNVLLTGETGVGKSLLAKIIHFTSRRQTMPFLSINCATLTEELLASELFGHEKGA
                     FTGAVRTKQGLVEIADTGTLFLDEIAEMAPNLQAKLLKVIEEGEFYRVGGTKPIKVDV
                     RFIAATNQNVRTVIAEGRFREDLYYRLNIMEIFIPPLRDRREDIPPLCEYFLEKHLPS
                     SRKKITGFTKGSMDVLMSYSYPGNVRELENIVERAIILEKTGLITPESLPQTIKMFQI
                     ETIEPDKVKTIEELNKDYAEKVIEMLGGNKSKAAELLGISRTSLWRILKEE"
     gene            3045..3335
                     /locus_tag="VFK23_00785"
     CDS             3045..3335
                     /locus_tag="VFK23_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317648.1"
                     /translation="MKKAAAVLAVVIMVVSLVSVVFAAEAKKGTIKGVDAKAGTITFC
                     AEGTTTDMTLKADKGVDLSKVKADTKAEIMVDKDTVKEIKEMKAKPKAAVGC"
     gene            3590..>5203
                     /locus_tag="VFK23_00790"
     CDS             3590..>5203
                     /locus_tag="VFK23_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HET7317649.1"
                     /translation="MATTYTAKDITVLEGLEPVRKRPAMYIGSTDARGLHHLVWEVLD
                     NSVDEALNGYCTEIKLTIERDGGVTVTDNGRGIPVDIHPKTKRPAIETILCTLHAGGK
                     FDHTAYKSSGGLHGVGASVVNALAEVFIATVWRDGFEWVQEFSRGKPKGSLKKGAATR
                     AHGTRIYFRPDPEIFSKTNFNYKNILDVSESKAFLNKGLKITIENSRDNEKPVTFKYD
                     NGIQDYIKRIVSGTTVVNTEPFYLEKEDDFKIECAIHWTLATDMELHSYANSINTSDG
                     GTHELAVRSGLTKAVRAYAERKNLIPKNVKAIAPEDVYEGLYGIVSVLIKNPQFQGQT
                     KEKLNNPEILSPIDASVKNAAEAYLLANPTMADAVVKRVLLAAEARLASRAAKESVTR
                     KLSSLKLNLPGKLADCSSGKVEETELFIVEGDSAGGSAKMARDRKTQAVLSLRGKILN
                     VEQVASLEKIQTNNEIKNLLATLGCGVGAHFDLGKLRYNRVILMTDADVDGAHISVLL
                     LTFIYRYMPELINHGHVYLAMPPLFKIEAG"
BASE COUNT         1258 a         1457 c         1516 g          972 t
ORIGIN      
        1 cgccggagag gccgtgatgg tccgcgacta cataggccag atcaaggcga ttcttgccca
       61 gcatccgcgt ttcggcgagg aggaccagcg tgcgctcggg atgggcatcg cggcctacca
      121 gggccgcttt gacagaatag attcgtcggc agcggaactt accgcggtct tcgaggacct
      181 gaagtccagg gacgggaaga acgcaaccta tctctccctc gtcgaggaga cattcctcga
      241 ccggccgggc caatcggcgt acttcctgac caaggttttc cgtatgcctg aacaccaccg
      301 tctggtagtg ggccttcgcc ggctggacgc ggatatcgcg gcgctcagga agaacggaga
      361 ggatatcctt gtaaccacaa aagacctcga caaggcggca cgggaaaagg cggagaccgt
      421 gatccgggtt tctcagcttg cgatcctcat cttctttccg ctgtttttcg ccgtgggcat
      481 cggcatgctg tttttcatca cggcgaacgt agtgaggagg cttatgcttc tcatcaccgt
      541 ggtggaaaaa acggggaagg gctcctatcc gcaccttacc gtggccccgt cggacggcag
      601 agcccgcgac gaggtgggcg ttctgataga aaagttcaac gacagggagg accagcttgc
      661 cctgcgggag caggagctgg gacgaaagaa ccaggaattg atgcaggcca agaagctggc
      721 ggcgatcgga acgctcgcgt cgggagtggc ccatgagctg aacaaccccc tcaataacat
      781 ttacctttcg gcccaggcgc tcacccggga tgcgggggag agctgttccc cggaagtgaa
      841 agaggcggtg ggcgacatcg tgggccagac ggagcgcgtg aagcgcatcg tcagcgacct
      901 tttggaattc gcccgcggga gggaacctca gtatcaggac gttgacctct gcgggctcat
      961 cacggacgcc taccggcacg ccgccagttc gcgggacctt tccgggatcc ggtatgcctg
     1021 ctcttccgac ccgttccgga tcatcgcttc gctcgaccag gggcagatgg agcaggtatt
     1081 caccaacctc tttctgaacg cggcggacgc catgcaggca aagggggaga ttaccgtgac
     1141 ggcgaagcga gaggcccaag acgtggtcat ccgggtctcc gacagcggca gagggatccc
     1201 ccgggaatca caggacaaga tattcgagcc ttttttcacg acgaaggaca agggaacggg
     1261 gctcgggctt gcgatcgtct ataatatcat caagaagcac gacggcgata tcaccgtcca
     1321 gagcgaagag ggaaaaggaa cgacgtttgt catcacgttg ccggttggta agaccccata
     1381 gtcgagtatt cctcttgacg tcattcccga atgattctat cgggaatcca ggtttttttt
     1441 catgctgtca aggtcaaaag cgccggattc cggctagaac catgccggaa tgactgcggg
     1501 aaaacattaa gggaaaaaga aatggccttc aagatcctta tagccgagga tgaagagatc
     1561 accctgaagc acctgatcaa tgcgctccag aaggccggct acacggccgt cggaacgcag
     1621 aacggacagg atgcattcca gaagatcgag aacgatcact tcgacctttt gatcgccgac
     1681 atcaaaatgc cgggcatgac cggcatggag ctcctcgaca agatcaagga gaggcagatc
     1741 gaggccgagg tcatcatcat caccggcttc ggcagcatcg gctccgtggt ggaggcgatg
     1801 aagaaggggg cgtacgacta cgtcaccaaa cccttcaacc tcgacgaact cttgctcaag
     1861 gtcggaaaga tccgggagca gcggaccttg aagaaggaaa acatcgccct caaggccttc
     1921 atgggcatgg accggaaggt atcgatcatc gccaagagcg cgagcatgaa caggatactc
     1981 gataccatcg acggaatgaa ggattcggac tgcaacgtgc ttttgaccgg ggagaccgga
     2041 gtgggcaaga gccttttggc aaagatcatc cacttcacga gccgccgcca gaccatgccc
     2101 ttcctttcga tcaactgcgc gacgcttacc gaggaactcc tggcctccga gcttttcggg
     2161 cacgaaaagg gcgccttcac cggcgcggtc aggaccaagc agggccttgt ggagatagcg
     2221 gacacgggca cgctgtttct ggatgagatc gcggagatgg ctccgaacct ccaggcaaag
     2281 ctgctcaagg tgattgagga aggcgaattc taccgcgtcg gggggacaaa gccgatcaaa
     2341 gtggacgtgc ggttcatcgc cgcgacgaac cagaacgtac ggacggtgat cgccgagggg
     2401 aggttccggg aagacctgta ctatcggctg aacatcatgg agatctttat ccccccgctc
     2461 cgcgaccgaa gggaggacat cccgccgctc tgcgaatatt tcctggaaaa gcacctcccg
     2521 agttcccgca agaagatcac ggggttcacg aaagggtcga tggacgtgct gatgagctac
     2581 agttatccgg gcaacgtgcg ggagcttgaa aacatcgtcg agcgggcgat tatactggaa
     2641 aagaccggtc tcataacccc tgaaagcctt ccgcagacca tcaagatgtt ccagatcgaa
     2701 accatcgagc ccgataaggt caagacgatc gaggaactga acaaggatta cgccgagaag
     2761 gtgatcgaga tgctcggcgg caacaaatcg aaggctgcgg aactcctcgg catctcccgg
     2821 acaagcctct ggcgaatcct gaaggaggaa tagaccgcgc cgccccaccg cgcactccgc
     2881 accttgccga ccggggccgg ctgttcaccc ttgtccctca cccttcgggc cgtaaaattt
     2941 ccaaatcgca tcatttaaaa ttttaattga gtaattctta aacttgtggt attgtatgtc
     3001 tccatggtca ttatcaatcc aaatccaaac aaggagggaa gaagatgaag aaagcagcag
     3061 cagttctcgc agtggtaatc atggttgtat ctctcgtttc tgtggttttc gcggctgagg
     3121 caaagaaggg caccatcaag ggcgtcgatg caaaagcagg cacgatcacc ttctgtgcgg
     3181 agggcaccac caccgacatg acgctcaagg ccgacaaagg cgtcgacctc agcaaggtca
     3241 aggccgatac gaaggctgag atcatggtgg ataaggacac ggtaaaagag atcaaggaga
     3301 tgaaggcgaa gcccaaggcg gcagtgggct gctgatcccg taacttcgat caagggagtc
     3361 gtccatccgg cggctccctt tttttatctt cagatcgccg atctcatgat ccccgcctgc
     3421 cactctgccc gcacatctta aacttcatgt tgctttatcg cccttcctga tttataatct
     3481 gccgcgaaaa gaccggttct cgggacatgg ctcatggagg caaagaaagc acctggcctc
     3541 ctgatagacc aattccggaa tgacggttga gaggcaaagg gagattcgtt tggcaactac
     3601 ctataccgca aaagacatca ccgttcttga agggcttgag ccggtccgaa agcggccggc
     3661 catgtatatc ggttccacgg atgcgcgcgg cctccatcat ctcgtgtggg aggttctcga
     3721 caactccgtc gatgaagccc tgaacggata ctgcaccgag atcaagctta ctatcgaaag
     3781 agacggcggc gtcacggtca cggacaacgg ccgaggcatt cccgtggata tccatcccaa
     3841 gaccaaacgg cctgccatcg agaccatcct ctgcacgctc catgcgggcg gcaagttcga
     3901 ccataccgcg tataaatcct caggtgggct gcacggcgtc ggcgcatcgg tggtgaacgc
     3961 tctggccgag gtgtttatcg ctacggtctg gcgcgacggg ttcgaatggg ttcaggaatt
     4021 ttcccggggc aagccgaagg ggagcctcaa gaagggagcg gccacgcggg cgcacggcac
     4081 gcggatctat ttccggcccg atccggagat attttcgaag acgaacttca attacaagaa
     4141 tatcctcgat gtgtccgaga gcaaggcctt cctgaacaag ggcctcaaga taactatcga
     4201 gaactcccgc gacaacgaaa agccggtaac cttcaaatac gacaacggga tacaggacta
     4261 catcaagcgc atcgtctccg ggaccacggt cgtgaacacc gaaccctttt acctcgagaa
     4321 ggaagacgat ttcaagatcg agtgcgccat ccactggacc cttgccacgg acatggaact
     4381 gcactcctac gcgaattcga tcaacacctc tgacggcggc actcatgagc ttgcggtccg
     4441 gtccgggctc accaaggccg tgcgtgccta cgccgaacgt aagaacctta tacctaagaa
     4501 tgtcaaggcc atcgcgcccg aggacgtata cgaggggctt tatggtatcg tgagcgtgct
     4561 catcaagaat cctcagttcc agggccagac caaggaaaag ctgaacaacc ctgagatact
     4621 gtctcccatc gatgcttcgg tcaagaacgc cgccgaagcc tatcttctcg caaacccgac
     4681 catggccgat gcagtggtga aacgcgtgct tttggccgcc gaggcgaggc ttgcgtcgcg
     4741 agccgccaag gaaagcgtga cgagaaaact gtcgtccctg aagctcaacc tcccgggcaa
     4801 gcttgcggat tgctcctccg gcaaggtgga agagaccgag ctgttcattg tggaaggcga
     4861 ttcggcgggc ggttctgcaa agatggcccg ggaccggaag acacaagccg tgctgtcgct
     4921 ccggggcaag attctgaacg tggagcaggt ggcctccctc gagaagatcc agacgaacaa
     4981 cgagatcaaa aacctgctgg ccacactcgg ctgcggtgtg ggcgcgcact tcgacctggg
     5041 caagctcagg tacaaccgcg tgatcctgat gaccgacgcg gatgtggatg gagcgcatat
     5101 cagcgtcctg ctgctgacct tcatttatcg gtacatgcca gaacttatca accacggaca
     5161 cgtctatctc gccatgccgc cgctgttcaa gatcgaggcc ggc
//
LOCUS       DASSLD010000044         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_483100,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000044 DASSLD010000000
VERSION     DASSLD010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483100"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFK23_00795"
     CDS             <1..460
                     /locus_tag="VFK23_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7317650.1"
                     /translation="SRIRYGLFGAFFGTTKRNKEEAEIEEKAYTLLEMMGIRHLSDQV
                     VLNLPYGAQRRVEMARALATEPRILLLDEPTAGMNPEELGQIMEIIRRVHDELGLAVL
                     LIEHRLKVVMELCERIQTLNFGEVIAEGTPEEIQNDPKVIDAYLGKESVI"
     gene            460..1173
                     /locus_tag="VFK23_00800"
     CDS             460..1173
                     /locus_tag="VFK23_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7317651.1"
                     /translation="MLLSVENLHVSYDNIEALHGLSFGIDEGEIVCIIGANGAGKSTT
                     LRAISRLVPVEAGTIMTFMGRNLLKYPADKVVSELGISHVPEGRRLFDNLTVLENLRL
                     ATFARRDGRAIGEDLDRVFSIFPRLSERQNQKAGTLSGGEQQMLAVGRAFMSGRKIML
                     LDEPSMGLAPLLMKSVFDSLKEINREGTTILVVEQNARMALQFAGRGYLLENGRVMLE
                     GRSEELLADPRVKKAYLGG"
     gene            1284..>2334
                     /locus_tag="VFK23_00805"
     CDS             1284..>2334
                     /locus_tag="VFK23_00805"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HET7317652.1"
                     /translation="MSYARPRAGVHPVTHHWRKITVADLGFSSATRMENGKLLIDKDE
                     LTTLLRSDPNIKDVQLAIARPGESVRIIPVKDIIEPRLSRIPGYPIFPGVLSAWDPDT
                     DGILSGEIVSLRGMVVTTVGTIVGFQEGVIDMSGPGARYSLFSGKHHLVLNISVKEGS
                     ERHEHEKSVRLAGFKASDYIARSSLNGPLSFDSRETLINPIDSGIDGSEKSVERNRKP
                     KIAYLYMLQSQGLLHDTYYCGVHVKKLLPRRIIPTEVLFGAIVSGNCVSACDKNTTYH
                     HQNNPIILELLRRHGKDWDFVGCVITNENVTLGDKQRSSTLAADLIASLNPDGVIVSK
                     EGFGNPDADLMMNCSK"
BASE COUNT          537 a          604 c          695 g          498 t
ORIGIN      
        1 ctcgagaatc cggtacggat tgttcggggc ctttttcggt acaacgaagc gcaacaaaga
       61 agaggcggag atcgaagaga aagcgtatac gctcctggaa atgatgggca tccgccacct
      121 ctcggatcag gtggtcctca acctgccata cggtgcccag agaagggtag agatggcccg
      181 ggcgctggca acggaaccca ggatattgtt gttggatgag cccacggcgg ggatgaaccc
      241 ggaagagctg ggtcagatca tggagatcat ccgtcgcgtc catgatgagt tggggctggc
      301 ggttctcctc atcgagcaca ggctgaaggt ggtcatggag ctctgcgaga ggattcagac
      361 cctgaacttc ggtgaggtca tcgccgaggg cacacccgaa gagattcaaa acgatcccaa
      421 ggtgattgat gcctatttgg gcaaggagag tgtgatctga tgttgctgtc cgtggaaaac
      481 ctgcatgtct cctacgacaa tatcgaggcc ctccatggtt taagcttcgg gatcgacgag
      541 ggggagatcg tgtgtatcat cggcgccaac ggagcgggga agagcaccac cttgcgagcg
      601 atttcccgcc tcgtccctgt cgaagcgggg acgataatga ccttcatggg ccggaacttg
      661 ctcaaatatc ctgccgacaa ggtggtcagc gagctcggaa tctcccatgt ccccgaagga
      721 aggcgtctgt tcgacaacct gacggtcctg gagaacctcc ggttggccac ctttgcaagg
      781 agggacggca gagcgatcgg ggaagacctg gaccgggttt tctcgatctt ccctcggctt
      841 tccgagcggc agaatcagaa ggccggaacc ctgagcggcg gcgagcagca gatgttggcc
      901 gttggtcggg catttatgag cggcaggaaa atcatgctcc tggatgagcc ctccatgggg
      961 ttggctccct tgttgatgaa gagcgtgttc gactcgctga aggaaatcaa tcgggaagga
     1021 accaccatcc tggttgtcga gcagaacgca cgcatggcgc tccaattcgc aggtagaggg
     1081 tatttgctgg agaacggcag agtcatgctc gagggcaggt ccgaggaact tctcgccgac
     1141 ccccgggtca agaaagcgta tctcgggggg tgatcgccgg gaaaaattcc ttcggtcata
     1201 ttgtagtggg atggcgatgg tatgctaatt atattggtgc cggcaaggaa tcgataaacc
     1261 ggcccgtcct gtaggaggga tttatgtcgt atgcgagacc tcgtgcgggt gtgcatcctg
     1321 taacgcatca ttggagaaaa atcaccgtcg cggatctggg gttttcctcc gccaccagga
     1381 tggaaaacgg gaaattgctc atcgacaaag atgagttgac caccctgtta cgatccgatc
     1441 ccaacatcaa agacgttcaa ttggcgattg cccgacccgg ggagtcggtt cgtattattc
     1501 ccgtcaagga tatcatcgaa ccccgcttga gcaggatccc gggttatccg atctttcccg
     1561 gagtgctttc cgcatgggat ccggataccg acgggatact ttccggggag atcgtgtcat
     1621 tgcgcgggat ggtcgtgacc accgtgggaa cgattgtggg ctttcaggaa ggggtcatcg
     1681 acatgtccgg cccgggcgca cgatactcgc tcttttccgg caaacatcat ctggtgctga
     1741 atatttccgt aaaagagggg tcggaacgac acgaacacga aaaatcggtc cggttggccg
     1801 gtttcaaggc atccgattac atcgcccgtt cctctttaaa tgggccgctt tccttcgaca
     1861 gccgcgaaac cctgatcaat ccaatcgatt cggggatcga cggttcggaa aaatccgtcg
     1921 aacgaaatag gaagcctaaa atcgcctatc tgtacatgct ccaatcccag ggccttttgc
     1981 atgacacgta ctattgcgga gtgcatgtca aaaagttgct gccgaggcga atcattccca
     2041 cggaagtgct gttcggcgcc atcgtctccg gaaattgcgt gtcggcgtgc gacaagaaca
     2101 cgacatatca tcaccagaac aaccccatca tcctcgaact cctgcggaga cacgggaagg
     2161 actgggactt cgtcggctgt gtcatcacca acgaaaatgt taccttgggc gacaaacagc
     2221 ggtcgtccac gctggcggcc gatctgattg catccttgaa tcccgacggt gtcatcgttt
     2281 ccaaggaggg tttcggcaat cccgatgccg atctcatgat gaactgttcc aaga
//
LOCUS       DASSLD010000045         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1331135, whole genome shotgun sequence.
ACCESSION   DASSLD010000045 DASSLD010000000
VERSION     DASSLD010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331135"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VFK23_00810"
     CDS             complement(<1..547)
                     /locus_tag="VFK23_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011021372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7317653.1"
                     /translation="MKASDLFVRQLEEEGVEYVFGLPGEENLDFLESLRTSKIKVIIT
                     RHEQTAAFMAATYGRLTGKAGVCFSTLGPGATNLVTGVAHAQLIGAPLVSISGQKAVR
                     ENRQARFQLIDIVSVMRPVTKEAVTIVDPGMIPTVLRNAFKLAQAERPGAVHVELPED
                     VAASSTSAPVQKRGFVRRSAPD"
     gene            complement(663..2168)
                     /locus_tag="VFK23_00815"
     CDS             complement(663..2168)
                     /locus_tag="VFK23_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET7317654.1"
                     /translation="MATRKAAVRKRKIKKETALPRVKRAASSGREVKKMKTSRLKTAV
                     RRPTKIQSINPYTEQVMQEFPLMSRQEVDWQLKASHDVFLTWRDTPVSERSNYLKKLA
                     GVLRSEKRNYAELMTKEMGKAIRESLAEVEKCAWLADYYSENTEPFLRPEEIRTEAKK
                     SYVLFQPLGVVLAIMPWNFPFWQAFRFGVPAVAAGNTIVLKHASNVPATALAIEDAFR
                     KAGFPDNVFKTLLISSSEALRLIDEDRVDAVSLTGSKAAGEEVGSHAGAKIKKVVLEL
                     GGSDPFIVLDDADIAKAGRMAANARMINAGQSCIAAKRFIVMEKVADEFTKHFMARLK
                     ELKIGDPMDETTDVGPLSRKDILQSLNDQLKDARAKDARVLQTDHAFKQGYFFAPCAV
                     VGPTENMKVLTEEVFGPIAPIITVKSEDEAVRVANNSEYGLGASIWSRDHDRAERLAA
                     RIETGTVAINDMVKSDPRLPFGGVKKSGIGRELSHYGLKEFVNIKTVVVRE"
     gene            complement(2217..>2426)
                     /locus_tag="VFK23_00820"
     CDS             complement(2217..>2426)
                     /locus_tag="VFK23_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HET7317655.1"
                     /translation="PVYHMMGIPHDLMTPVFAVARIAGWTAHIIEEKFGDAQGKPMLY
                     RPSSVYVGNYCGKTGCVYEPLERRK"
BASE COUNT          423 a          784 c          666 g          553 t
ORIGIN      
        1 cgtccggcgc ggacctgcgc acgaaccccc gcttctgtac cggcgcgctg gtagaacttg
       61 ccgcgacgtc ctcgggaagt tccacatgca cggcgccggg ccgttcagcc tgcgcaagct
      121 tgaaggcgtt ccggaggacc gtcgggatca tgccgggatc gacgatggta accgcctcct
      181 tggtgacggg acgcatcacg gacacgatgt cgataagctg gaaacgggcc tgtcggtttt
      241 cccgcaccgc cttctgtccc gagatggaca cgagcggcgc accgatgagc tgcgcatgcg
      301 cgacgccagt caccaggttc gtcgcgccgg ggccgagggt cgagaagcat acacccgcct
      361 ttcccgtcag ccggccgtac gtggcagcca tgaaagccgc ggtctgttcg tggcgcgtga
      421 tgataacctt gatcttcgac gtgcggagcg attcgagaaa atcgaggttc tcctcgcccg
      481 gcaggccgaa gacgtactcg accccttctt cctccagctg cctcacgaac aggtcggaag
      541 ccttcatgaa gtctccttag aaaataacga aggacgatgg acgaccgctc acttcgttcg
      601 ctacgacgac ggacgaaaac agcttttgca tccctcgtct ctcgtccctc atccctcgtg
      661 atttattccc tcaccaccac cgtcttgatg ttcacgaatt ccttcagccc gtagtgcgac
      721 agctcgcggc cgatgcctga cttcttcacg cctccgaagg gaagccgggg gtcggatttc
      781 accatatcgt tgatcgcgac ggtccccgtc tcgatccgcg cggcaagccg ttcggcccgg
      841 tcatggtccc tgctccagat cgaggcgccg agcccgtact cgctgttgtt cgctactctt
      901 accgcttcat cctcgctctt cacggtgatt atcggcgcga tcgggccgaa gacctcctcg
      961 gtcaggacct tcatgttctc cgtggggccc acgaccgcgc agggcgcgaa gaaatacccc
     1021 tgtttgaacg cgtggtcggt ctgaagcacc ctggcgtcct ttgccctggc gtccttgagc
     1081 tgatcgttca gggactggag gatgtccttc cgggacagcg gtccgacgtc cgtcgtttca
     1141 tccatggggt cgccgatctt gagctccttc aatctcgcca tgaagtgctt cgtgaactca
     1201 tctgcgacct tttccatgac aatgaagcgc ttcgccgcga tgcagctctg gcccgcgttg
     1261 atcatacgcg cattcgcggc catcctgccc gctttcgcga tgtccgcgtc gtcgagcacg
     1321 atgaaggggt ccgagccgcc cagttcgagc acgaccttct ttattttcgc tcctgcatgc
     1381 gagccgacct cttcaccggc cgctttgctg ccggtaagcg aaaccgcgtc cacccggtcc
     1441 tcgtcgatga gcctcagggc ctccgacgaa ctgatgagga gggtcttgaa cacgttgtcc
     1501 gggaatcctg ccttcctgaa cgcatcttca atcgcaagtg cggtcgcggg gacgttcgac
     1561 gcatgcttca aaacgatcgt gttccctgct gcgactgccg gtacgccgaa ccggaaagcc
     1621 tgccagaagg gaaagttcca gggcatgatc gccagaacca cgcccaacgg ctggaacagg
     1681 acgtagcttt tcttcgcctc tgtcctgatt tcctcgggcc tcagaaaagg ctccgtattc
     1741 tccgaatagt agtccgcgag ccacgcgcac ttctcgactt ccgcgagcga ttccctgatg
     1801 gccttgccca tttctttcgt catgagttcg gcataattgc gtttctcgga ccggagcacc
     1861 cctgcaagct tcttcaggta attcgaccgc tcggagacgg gcgtgtcgcg ccacgtgaga
     1921 aagacgtcat gggaggcctt gagctgccaa tcgacctcct gcctgctcat gagtgggaat
     1981 tcctgcatca cctgttcggt gtaaggattg atcgactgta tcttcgtcgg cctcctcact
     2041 gccgttttca gacgactggt tttcattttt ttcacctccc gtccggagga cgcggcgcgt
     2101 tttaccctgg gaagcgcagt ttcctttttt attttccttt tccgcaccgc tgcttttctg
     2161 gttgccatcg gcgtcctccg tcaagtcaaa atcaaattcc aacttggtgc ttgtgttcac
     2221 ttcctccgct cgagcggttc atagacgcag ccggtctttc cgcagtaatt gcccacatac
     2281 acggacgacg ggcggtacag catgggcttc ccctgcgcgt caccgaactt ctcctcgatg
     2341 atgtgcgcgg tccaaccggc gatccgtgcc acagcgaaga ccggcgtcat cagatcgtgc
     2401 ggtatgccca tcatgtggta gaccgg
//
LOCUS       DASSLD010000046         3549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_124307,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000046 DASSLD010000000
VERSION     DASSLD010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124307"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFK23_00825"
     CDS             <1..302
                     /locus_tag="VFK23_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317656.1"
                     /translation="KSDAAYRRTVLPDEAVSRACMEGRALLRASLRERILLLTSQHVS
                     GMKRLVAKCIVMLIQKKEKCKRRSGLLSINANYDAMKDSYMSLLKTSIDRRDCFC"
     gene            495..743
                     /locus_tag="VFK23_00830"
     CDS             495..743
                     /locus_tag="VFK23_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HET7317657.1"
                     /translation="MALKDEAPKETLDTLGRVCPYPLLLTKKALEKLGSGQTLKVLCD
                     APASAEDSIPRYAEKQGYTFESVKIEDKGMWELYIKKK"
     gene            920..1225
                     /locus_tag="VFK23_00835"
     CDS             920..1225
                     /locus_tag="VFK23_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317658.1"
                     /translation="MAQEMLGIMVTKYENVEHIAGIAKAARAAGKGVVIFLTDEAVRF
                     TTDPKFLELLEVGGVELSCCDHSCEKVGVHEKTDGITYGSQYNNATMLHDSARVLVF"
     gene            1256..1534
                     /locus_tag="VFK23_00840"
     CDS             1256..1534
                     /locus_tag="VFK23_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317659.1"
                     /translation="MGDTKKIAVVVRDRQSEALRVAGGLTLADDTIEVYILDRKLDKD
                     NPEVAMPLELVTDLDLKVYSNNPENGYTTLSLEEMAKKLLEYDVVVPY"
     gene            1552..1722
                     /locus_tag="VFK23_00845"
     CDS             1552..1722
                     /locus_tag="VFK23_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317660.1"
                     /translation="MKILHILKKAPDESTRKIMELQAPGNEVKTVELYKGGVSYDKLI
                     ADVFAYDKVFCW"
     gene            2056..2979
                     /locus_tag="VFK23_00850"
     CDS             2056..2979
                     /locus_tag="VFK23_00850"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET7317661.1"
                     /translation="MICAVCSFENDADALFCADCGKSLVDQAGPASKKSRPYFIGVLF
                     AAVIILVAAGYYKFILPDGVAAVVNGEKITLSELDAAVSRMGGTTGGAADGLRYRALD
                     ELIAERLVLQEAGKAGIRISGDETASAARDARAASGLDDAAFTSQMKAFYGSTRDFQK
                     ALERRLVINRYLAERIVPRGAGPQAAAQAVDQWLRRLTAKASVRIALAENLPAPCGGC
                     RGHGPSGGQPSQTCSRPGRGCQAGNSRVESTIASKAAEEAVLRYWHMKHGPDAVSARL
                     QDFGCHIQVEIVKDEKIIGSLRYQDGIITEM"
     gene            2996..>3549
                     /locus_tag="VFK23_00855"
     CDS             2996..>3549
                     /locus_tag="VFK23_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing protein"
                     /protein_id="HET7317662.1"
                     /translation="MSKCGKCGKEVKTNAKFCQACGAGLLVGAGALKDKKARVMARER
                     PWKKQLAIAGAVLLVVALGWTAKGVYMAKKMGDRPTFPPRREESSRLAAAERVNERSG
                     SVSVPLAALEDGKAHFFAFATGGTTITFFAMKAADGSIRTAFDACMACNHAKLGYRPE
                     GGLVVCNNCGMGFKPAEIGVETGG"
BASE COUNT          847 a          900 c         1108 g          694 t
ORIGIN      
        1 tgaaaagcga tgctgcgtat cgaagaacgg tgttacctga cgaggctgta tcacgcgcat
       61 gcatggaagg ccgggcattg ttgcgcgcgt ccttgaggga aagaatatta ttgctgactt
      121 cgcagcatgt ctccggcatg aaaaggcttg ttgcgaaatg catcgttatg ctaatacaaa
      181 aaaaagaaaa atgcaagagg agatcggggt tgctatctat aaatgccaat tatgatgcaa
      241 tgaaagattc ttatatgagc ctgttaaaaa cgagtattga caggcgggac tgtttttgtt
      301 aaggtgatac accgttcgcc agctggtgaa tccccgttga cctgaaagag ctgaatgcag
      361 gggcctttct ccgttcggcg ccatttcctt ttcgttttcc ttccggacat gacggggaca
      421 gttttctgct gaacttgcat acgggcaata tcgatggccc gaatttgata ctcattattt
      481 gaggaggaac tatcatggca ctgaaagatg aagcacccaa ggaaacgttg gatacgctcg
      541 gaagggtatg cccttatccg ctcctgctta ccaagaaggc gctggaaaaa ctcggcagcg
      601 gccagacgct caaggtgctc tgtgacgcgc ccgcgtccgc agaggacagc atccctcgct
      661 atgccgagaa gcagggctac acgttcgagt ccgtgaaaat cgaagataag ggaatgtggg
      721 aactctacat caagaagaag taattgtgca ggacgtccat aagccgttct tggcgtctcg
      781 caagaggctt cgcgtcgtgt tcctgaagca tgcatgactg caccctgagc cgggctgagc
      841 atccgcccgg cttttttgtg gcgccgaaca ttaataaaac atgtgttaga atcctgagaa
      901 tgggaaggag gtgaccgaca tggctcaaga gatgctgggc atcatggtca cgaagtatga
      961 aaacgtggag cacattgcgg gcattgcaaa ggcggcgcgg gcagcgggaa agggcgtcgt
     1021 gatcttcctg accgatgaag cagtgcgttt taccacggac ccgaagtttc tcgaattgct
     1081 ggaggtcgga ggcgtggagc tgtcctgctg cgaccacagc tgcgagaagg tcggcgtgca
     1141 tgaaaagacc gacggaataa catacggcag ccagtacaat aatgcaacga tgctccatga
     1201 cagcgcgcgt gtactggtgt tttaaaaaca atccgaggac aaggagattc gggacatggg
     1261 agatacgaaa aaaatagcgg tggtcgtgcg ggaccggcag agcgaagcgc tccgagtggc
     1321 gggcggtctc acgcttgccg acgacacgat cgaagtctat atcctggacc ggaaactgga
     1381 caaggacaac ccggaggtgg cgatgccgct cgagctggtc accgacctgg acctcaaggt
     1441 ctattcgaac aacccggaaa acggctatac caccctatca ctcgaggaga tggcgaagaa
     1501 gctccttgaa tatgacgttg tggtgccgta ttaaaagcga aggagagcac gatgaaaatt
     1561 ctgcatattt tgaagaaggc gccggacgag agcacgagga agatcatgga actgcaagcc
     1621 cccggcaacg aagtcaagac cgtggaactg tacaaaggcg gcgtctcgta cgacaagctc
     1681 atcgcggacg ttttcgcata cgataaggta ttctgctggt aaggcgtttt ggcagcgcaa
     1741 tcccgcgggc gggtttgccc ggaacgggaa gataccagga ggaccggtgc gggggaacac
     1801 gcacggtcct tattgttcgg cgctgcgtgg aaatcgcaca tgacgacgct ttcgtggcac
     1861 acctgacact catcactcaa tcatctgcaa tctatttctg ttttctttcc tgcctctgct
     1921 gtatcaaatg ccacagcctt tcgccctttc cgcaactcat cccattgatt ttccggataa
     1981 agaggtcctg gcagggggat tgcaccatat gatggactga gcaacatcaa taaacaattg
     2041 gcgggagttt ttaacttgat ctgtgcggta tgcagcttcg aaaacgatgc cgacgcgctc
     2101 ttctgcgcag actgcggaaa gtcccttgtg gatcaagctg gccctgccag caaaaaaagc
     2161 aggccgtatt tcatcggcgt cctgtttgcg gcggtcatca tcctggttgc cgcgggctac
     2221 tacaaattca tactgccaga cggcgtcgcg gcagtggtga acggcgagaa gattacgctg
     2281 tcggagctcg atgccgctgt ctcgcgcatg gggggaacga cggggggcgc ggccgacggg
     2341 ctgcgatata gggcgctcga cgagttgata gccgagcggc tcgtgctcca ggaagcgggc
     2401 aaggcgggca tccgcatatc aggcgatgag acagcgtcag ccgcccgaga tgctcgggct
     2461 gcatcaggcc tcgatgacgc cgcttttacc tcgcagatga aggccttcta cgggagcacg
     2521 cgggattttc aaaaggcgtt ggagcgcagg cttgtcatca accgctatct cgctgaaagg
     2581 atcgtgccgc ggggggccgg tccgcaagcc gcggcacagg cggtcgatca atggctgcgg
     2641 cgcctgacgg ccaaggcatc ggtgcgaatt gcgttggcgg aaaatctccc ggcaccgtgc
     2701 ggcggctgcc gcggccacgg cccatccggg gggcagccct cgcagacttg cagccggcca
     2761 ggtagaggat gccaggcggg aaacagccgg gttgagtcga ccatcgcgtc aaaggctgcg
     2821 gaggaggcgg ttctccggta ctggcatatg aagcacggac cggatgccgt atccgcccgg
     2881 ctgcaggatt tcgggtgcca tatccaggtc gagatcgtca aggacgaaaa gatcatcggt
     2941 tccctcaggt accaggacgg aatcataacg gaaatgtgat gaaggagatc ggaacatgag
     3001 caaatgcggt aaatgcggaa aagaagtaaa aacgaacgcg aaattctgcc aggcctgcgg
     3061 tgcgggtctt ttggtgggag ccggggcgct gaaggacaaa aaagcgcgcg tgatggcacg
     3121 ggagcggccg tggaagaagc agttagcaat tgccggagcg gtcctgctgg tagtcgccct
     3181 gggatggacg gcaaagggag tctacatggc gaaaaagatg ggcgaccgcc cgacgtttcc
     3241 cccccgccgt gaagagtcgt cgaggctggc ggccgccgag cgcgtcaacg aacggagtgg
     3301 cagcgtaagc gtgccgctcg cagcactcga ggacggcaag gcccatttct tcgccttcgc
     3361 gacgggcgga acgacgataa cctttttcgc catgaaggcc gcggacggaa gcatccgtac
     3421 ggcctttgac gcctgcatgg cctgcaatca cgccaaacta ggataccggc ccgagggcgg
     3481 cctcgtcgtc tgcaacaact gcggtatggg tttcaagccg gcggaaatcg gcgtggagac
     3541 cggcggttg
//
LOCUS       DASSLD010000047         3491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1266044, whole genome shotgun sequence.
ACCESSION   DASSLD010000047 DASSLD010000000
VERSION     DASSLD010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266044"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VFK23_00860"
     CDS             complement(<1..1025)
                     /locus_tag="VFK23_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET7317663.1"
                     /translation="MKRLTTDLLCDILIKAGKITPAQAKEITVKQDVQRMKLLKARGE
                     TLHRGRVHIEDEVSAIEVAASLNLEMPGAPGQVLTEDIITEAVAAHAGLPFKKIDPLK
                     LNPETTTQILSRAFARRSISVPIERDNDSLTLAVADPYNIEVVENIECMGYKVRRVVS
                     PKTDILKIITEFYGFRSSVTAAAKELSGEVDLGNLEQFVKMKSVSEIEATDRHVVNAV
                     EFLFRYAFDQRASDIHIEPKRDASEVRLRIDGVLNTIHRIPKAVHPAFVSRVKMLSRM
                     DIAEKRRPQDGRIKTEYKSREVELRVSTMPTAFGEKVVIRIFDPDILLQDLEEIGFEP
                     REYELFRS"
     gene            complement(1175..1387)
                     /locus_tag="VFK23_00865"
     CDS             complement(1175..1387)
                     /locus_tag="VFK23_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317664.1"
                     /translation="MKRLSAFLLILVMSITVGLYGCEKKAQEAPKPAQQPAMTPPPAP
                     APTPVAPAAPAPAPAPAPEPAKPAKK"
     gene            complement(1928..>3491)
                     /locus_tag="VFK23_00870"
     CDS             complement(1928..>3491)
                     /locus_tag="VFK23_00870"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET7317665.1"
                     /translation="PLGLRVMEKINRIIREEMNAIGGQEVTMPVLNPAEVWQQTGRWD
                     AIGDEMFRLKDRSGRDMCLAMTHEETMTWLAAHDLRSYRELPQIWYQIQTKLRDEARP
                     KSGVLRTREFTMKDSYSFDRDEDGLDKSYQLHLEAYKKIYSRCGIRFYVVQSDTGMMG
                     GSMAHEYMAPSAAGEDDVALCDGCGYAANVELALSAPKVSKSPGWQYEEIATPEKRTI
                     EEVTRFLKVTPDLLIKSILVVSDSGPVLALVRGDQTLHEKKLAKLIGPHRPAQKDEVK
                     SFLGVPAGFIGPMGHTIRKIADPALKQGVYAAGANKEGYHVRGVSAEQHFADAEFADI
                     HVAAAEDGCVQCGKALRIDRVIEIGNIFKLGTKYSAPLKANFLDEDGNEKPIIMGSYG
                     IGPARIAAAAVEQGNDENGIIWPAAIAPYQVIVLPVNVKDVKSMDLAEEMYTALEEKG
                     FEALLDDRDERAGIKFKDADLIGIPWRIVIGEKNLRDGLVEIKERRTGVVEKVRVEAA
                     VEAIAKKLVMET"
BASE COUNT          622 a         1052 c         1016 g          801 t
ORIGIN      
        1 gagcggaaca gctcgtactc ccgcggttcg aacccgattt cctcgaggtc ctggagcagg
       61 atatcggggt cgaagatcct gatcacgacc ttctcgccga aggccgtcgg catggtggac
      121 acgcggagct cgacctcccg ggacttgtat tccgtcttga tcctgccgtc ctggggccgg
      181 cgcttctcgg cgatgtccat gcgcgagagc atcttgacgc gcgagacgaa ggccggatgg
      241 acggctttcg gtatccggtg gatggtattc agcacgccgt cgatcctgag gcgcacctcg
      301 ctcgcgtcgc gctttggttc gatgtggata tcgctggccc tctggtcgaa ggcgtagcgg
      361 aagaggaact cgacggcatt gacgacatgc ctgtccgtgg cctcgatctc ggacacggat
      421 ttcatcttga cgaattgttc gaggtttccg aggtcgactt ccccggacaa ttccttggcc
      481 gccgcggtca ccgaagaacg gaacccgtag aattccgtga tgatcttgag gatgtcggtc
      541 ttcggggaaa cgacgcgccg caccttgtag cccatgcatt cgatgttttc gacgacttcg
      601 atgttatagg ggtccgcgac ggcgagggta agactgtcgt tgtcgcgttc gatgggcacg
      661 gaaatgctcc tccgcgcaaa ggcccgcgac aggatctgcg tcgtggtctc gggattcagt
      721 ttgagcggat cgattttttt aaacggcagc ccggcgtggg cggccacggc ttcggtaatg
      781 atgtcttcgg tcaggacctg gcccggggcc ccgggcatct ccaggttcag cgaagcggcc
      841 acctcgatgg ccgatacctc atcttcgata tgcaccctgc cgcggtgcag ggtttctccc
      901 ctcgccttca ggagtttcat ccgctgaacg tcttgtttga ccgtgatctc cttggcctgc
      961 gcgggcgtaa tctttccggc cttgatcagg atatcgcaga gtaggtcggt cgtcagtctt
     1021 ttcataacaa tgctgttccg gactcctcaa tcgggaagaa tcaatcgtcc cgcattatac
     1081 acgcaacagg acgaatgcac catgaaatat aaaaggcccc cggaagaggg gggccgtctt
     1141 ggatccagat gagccgggtc cggcgttcaa tacgctactt cttggccggc tttgcaggct
     1201 cgggcgccgg ggccggcgcg ggagccggag cagcgggcgc gacgggtgtc ggggccggcg
     1261 ccggcggcgg cgtcatagca ggctgttgag ccggcttagg ggcctcctgg gccttctttt
     1321 cgcagccgta caggccgacc gtgatgctca tgaccagaat cagaaggaac gctgacaatc
     1381 ttttcacaaa cgctcacctc cttcccgatg gttatcagat tctcgaataa gataaaatac
     1441 atccggcccg gcaggatatg cggagcgcat tcgccgccgg gggtgaaacg gctgctctcg
     1501 aggatcctcg ttgcaatcga ccgtgttacg gtcagttccc tcactcgctg gccgagtttt
     1561 tcctccaaaa ccccctgctg ttcatcgttc agacgaagac gaaaaacccg gagcttgttc
     1621 agtgactttt tcgtaagatg gatctcgcga agctcaaaac cgatctgtct ttttatttca
     1681 gcgctctgct gcggggtcaa ttcaagcttt acgaaatctt ccacggttga cgccctccat
     1741 gattaatacg taagtagcga ctattatagg cttattaccg cgcgaattca agccctattt
     1801 gcgcgccccg gagtaccggc gggttcagtt caaagttcaa agttcagagt tcagagtaaa
     1861 aaaccaagta aggataaggg caaatagagc ctttcgtatt atgaactttg aactctgaac
     1921 tgttttttta tgtttccatc accagcttct tcgcaatggc ttcgacagcc gcttcaaccc
     1981 ttactttttc cacgacgccc gtccttcgtt ccttgatttc cacgagcccg tcccggaggt
     2041 tcttctcgcc gatgacaatg cgccagggaa tgccgatgag gtcggcgtcc ttgaacttga
     2101 ttcccgcgcg ttcgtcgcgg tcgtcgagca gtgcctcgaa ccccttttcc tcgagcgccg
     2161 tatacatctc ctcggctaga tccatggatt tcacgtcctt gacgttcacc ggaaggacga
     2221 tgacctggta gggcgcgatg gcagccggcc agatgattcc gttctcgtcg ttgccctgct
     2281 cgaccgcggc cgccgcgatg cgcgccgggc cgatgccgta gctgcccatg atgatgggct
     2341 tctcgtttcc gtcctcgtcg agaaaattcg ccttgagcgg cgcggagtac ttggtgccga
     2401 gcttgaagat gttgccgatt tctatcaccc ggtcgatcct gagcgccttc ccgcactgca
     2461 cgcagccgtc ttccgcggcg gcgacatgga tatcagcgaa ctcggcgtcg gcaaagtgct
     2521 gctccgccga gacgcccctg acgtgatagc cctccttgtt cgcgccggcg gcatacaccc
     2581 cctgcttcaa cgccgggtcg gcgatcttcc ggatcgtatg acccatgggt ccgataaagc
     2641 ctgcgggcac gccgaggaag gacttcacct catccttctg cgccggccgg tgcgggccga
     2701 tgagtttcgc gagtttcttc tcgtgcagcg tctggtcccc ccggaccagg gccagcaccg
     2761 ggccgctgtc gctcacgacg aggatggatt tgatcagcag gtccggcgtg accttgagga
     2821 agcgcgtcac ctcctcgatg gtcctctttt ccggcgtcgc aatctcctca tactgccagc
     2881 ccggggactt cgaaacctta ggcgcggaca gcgcaagctc gacgttcgcc gcgtacccgc
     2941 agccgtcgca gagcgccacg tcatcctctc ctgccgccga cggcgccatg tactcgtggg
     3001 ccatggagcc gcccatcata ccggtatcgc tctgaaccac gtagaacctg atgccgcagc
     3061 ggctgtaaat ttttttatac gcttccaggt gaagctgata ggatttgtcg aggccatcct
     3121 cgtccctgtc gaagctgtag gagtctttca tggtgaattc acgcgtccgt aagaccccgc
     3181 tcttcggcct ggcctcgtcg cggagcttgg tctggatctg gtaccagatc tgcgggagtt
     3241 cacggtagga gcggaggtcg tgggccgcga gccaggtcat ggtttcctcg tgcgtcatgg
     3301 cgagacacat gtcgcggccg ctccggtcct tgaggcgaaa catctcgtcg ccgatagcgt
     3361 cccaccggcc ggtctgttgc cagacctcgg cgggattgag gaccggcatg gtcacttcct
     3421 ggccgccgat ggcgttcatc tcctcgcgga tgattcggtt tatcttttcc atgaccctga
     3481 gccccagcgg g
//
LOCUS       DASSLD010000048         4321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1334839, whole genome shotgun sequence.
ACCESSION   DASSLD010000048 DASSLD010000000
VERSION     DASSLD010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334839"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VFK23_00875"
     CDS             complement(<1..666)
                     /locus_tag="VFK23_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7317666.1"
                     /translation="METGYLLALTTGIVGGFGHCIGMCGPLVASYALASPADRLPLSA
                     QLTHHLLYNMGRITTYGLVGAFMGLSGSFVNSAGRLAGIQNAVAVMAGLLMIIMGMSI
                     AGILGPAAWIEKHNISVLRTARRFTGSPSAFRFYPLGLLLGLLPCGLSYTIFIASAGT
                     GGMLSGAATSLLFGFGTLPALLLFGTLFSSLTPSLRGGLYKAGGILVIAMGIYYLARG
                     MKLY"
     gene            complement(668..1516)
                     /locus_tag="VFK23_00880"
     CDS             complement(668..1516)
                     /locus_tag="VFK23_00880"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HET7317667.1"
                     /translation="MRLYIAILVTVCILALAATIGTIIVGTRSFEGIVVDKPYETGLA
                     WDETERQKAKLGWKVDGEGSRFRTGENELIIDAVDRRGKPLEDAVVTATVSRPSTRDL
                     DKTYAAVRQKSGRYRALVVFPLYGAWDITIDVSRDNDRGVYRNTVYAEPASGGTAGTA
                     AETKTLCNVQAGPCRGQTGDGLSIEFDIEPKPVTAMSDLSFAVALTRRGTPAADVSSV
                     MLDLSMPGMFMGKNQPSMKSAGAGRYEGKGVITRCASGKKTWQAEVTVKRPKRTDVVD
                     FIFEVN"
     gene            complement(1528..2745)
                     /locus_tag="VFK23_00885"
     CDS             complement(1528..2745)
                     /locus_tag="VFK23_00885"
                     /inference="COORDINATES: protein motif:HMM:NF023045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7317668.1"
                     /translation="MMMIRKFHTIRRITGAALLIILIVTPFLRIGGESAFRFDVPSLR
                     LLFFGAEIWMTDFFIVLIALLFLTFITLFTTTVFGRIWCGWMCPQTVLSDATAFVETA
                     QKRGTGAKFTASAAALAISALVSASLIGYFVSPYDLPALLANGGASARIATGSWIILA
                     LVLFLDTITVRRRFCATVCPYAKLQGVLFDERTLLVAFDPSRAGECMECGACVKACPV
                     GIDIRKGTQPACIHCAECIDACTAKMAARKRKSLVGYAFGLSGEKSGGLRLTPLITGV
                     LTVFSLVFLVYLASTQVPFDMNVRRDYRAVPVIAANGGVTNTFELTLRNTGRSDLELG
                     LVAAAPAGVVRVTPEAVILPRGTDVLRLPVSVTLTGHQGPKGHSVTVTLTARLRDGNS
                     LSKKAFFPLPGHE"
     gene            complement(2732..2875)
                     /locus_tag="VFK23_00890"
     CDS             complement(2732..2875)
                     /locus_tag="VFK23_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HET7317669.1"
                     /translation="MEKQAWAYFLFGLALVVLFVIIIAHYYSGKRRNKVEEAKHKMLD
                     DDD"
     gene            complement(3115..3300)
                     /locus_tag="VFK23_00895"
     CDS             complement(3115..3300)
                     /locus_tag="VFK23_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HET7317670.1"
                     /translation="MADEYDNFPGFEVKDSAKKLPVGWLILFWGLILWGIYYTAAYTP
                     AVSGWSQDKAYTESLKP"
     gene            complement(3311..4246)
                     /locus_tag="VFK23_00900"
     CDS             complement(3311..4246)
                     /locus_tag="VFK23_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HET7317671.1"
                     /translation="MAHYLENKPILFAIVAAVVILIGTAVTMFLPMLMDDMHPKLANL
                     KPYTPLQLAGKDIYQREGCNNCHTQTVRPLKTEVMRYGEYSKAGEFAYDRPFLWGSKR
                     TGPDLARIGGKYPDQWHQIHFGNPQAMYAESNMPAYGWLKSNTLDPQDIASHMKANSL
                     PYASDDIAALKGKTELDALIAYIQAIGTAVPRTAAAATPPVIKEKHFPNPLAGDPVAI
                     NQGAALYKQHCAACHGEKGQGGIGPSLIDDVFLYESGDMPDDDYFELINNGTQAGMVE
                     DGRTAKGGMPSFANAIEKNKIWALVSYIRSLQGKK"
BASE COUNT          881 a         1264 c         1302 g          874 t
ORIGIN      
        1 ataaagcttc attcccctgg cgagataata gattcccatg gcgatgacga gtatgcctcc
       61 tgccttgtag agcccgcctc ggagggatgg agttaaggac gagaacaggg tgccgaaaag
      121 aaggagcgcc ggaagtgtgc cgaatccgaa cagcagggag gtcgcggcgc cgctcagcat
      181 accgccggtc cccgccgacg cgatgaagat ggtgtacgaa agcccgcacg ggagaagccc
      241 cagcaggagg cccaggggat aaaaccggaa agccgaaggc gacccggtga aacggcgcgc
      301 cgtacgaagg acggaaatat tgtgcttttc aatccatgca gccgggccga ggatgcctgc
      361 gatgctcata cccatgatga tcatgaggag gccggccatc acggccacgg cattctggat
      421 gccggcgagc ctgccggcgc tgttgacgaa cgaaccggaa agccccatga aggcgcctac
      481 gagtccatag gtggttatgc ggcccatatt gtacaaaagg tggtgagtga gttgggcgga
      541 aaggggaagg cgatcggcag gggacgcgag cgcataggac gccacgagcg ggccgcacat
      601 gcctatgcag tgtccgaacc ctccgacgat gccggtcgtc agcgccagga gatagcccgt
      661 ctccacgtca gttcacctcg aaaatgaaat cgaccacatc ggtcctcttc ggccgcttga
      721 cggtcacttc agcctgccat gtctttttgc cgctcgcgca tcgtgtgatt acgcctttgc
      781 cttcgtagcg tccggccccc gcgctcttca tggacggctg gttcttcccc atgaacattc
      841 ccggcatgga aagatcgagc atgaccgatg agacatcggc ggccggcgtt ccccggcggg
      901 tcagtgcaac ggcaaacgaa aggtccgaca tggcggtcac gggtttaggc tcaatatcga
      961 attcaatgga taggccgtcg cccgtctgcc cgcggcacgg cccggcctgg acattgcaca
     1021 gggttttggt ttcagcggcc gtcccagcgg tgccgcctga tgccggttcg gcatacacgg
     1081 tattccggta aacgccgcga tcattgtccc ggctgacatc gatggttatg tcccacgctc
     1141 cgtagagagg gaaaacgacg agcgcgcggt atcgaccact cttttgtcga acggcggcat
     1201 aggtcttgtc gaggtcccgg gtcgaaggcc tgctgaccgt tgccgtcacg accgcatcct
     1261 cgagaggctt acctcttctg tcgactgcat cgataatgag ctcgttttct cctgtcctga
     1321 accgactgcc ctccccgtcg accttccacc ccagctttgc tttctgccgt tccgtctcat
     1381 cccacgcgag gcccgtctca tagggcttgt caacgacgat gccttcgaaa cttctcgtgc
     1441 cgacgatgat tgtcccgatc gttgcggcga gggccagaat gcacaccgtg accaggatcg
     1501 caatataaag gcgcatcgta atggtcctca ctcatgcccc ggcaacggga aaaaagcctt
     1561 cttcgagaga ctgttgccgt cgcgcagccg ggcggtcagc gttaccgtca cggaatgccc
     1621 tttcgggccc tgatggccgg tgagcgtcac cgaaaccggc aatctcagga catccgtgcc
     1681 ccttggcaga atgacggctt cgggggtcac gcggaccacg ccggccggcg ctgcggcaac
     1741 caggccgagt tccaggtcag acctgcccgt attgcgcagc gtcagttcaa aggtgttcgt
     1801 gacgcctccg ttcgcggcaa tcaccggcac agcccggtag tccctgcgta cgttcatgtc
     1861 gaacggcacc tgtgttgagg cgagatatac aaggaacaca agagaaaaaa cggtgaggac
     1921 gccggtgatg aggggggtca ggcgaaggcc gccgcttttt tcaccgctga ggccgaaggc
     1981 atacccgaca agcgatttgc gttttcttgc ggccatcttt gccgtgcatg cgtcgatgca
     2041 ctcggcgcag tggatgcacg ccggttgagt gcccttcctg atgtcgatgc cgacggggca
     2101 ggccttgacg catgctccgc actccatgca ttccccggcg cggctcgggt cgaacgccac
     2161 aaggagcgtc cgctcgtcga agagaactcc ctgcagcttc gcgtaggggc agaccgtggc
     2221 gcagaagcgc ctgcgcacgg tgatggtatc gagaaacagg acgagcgcaa ggatgatcca
     2281 ggacccggtc gcgatacgcg cggacgcgcc gccgttcgcg agcagcgcgg gaaggtcata
     2341 gggagacaca aagtacccga tcagactcgc ggagacgaga gcgctgatcg ccagagccgc
     2401 ggcggaagcg gtaaatttcg cgcctgttcc ccttttctgg gcggtttcca cgaaggcggt
     2461 cgcatcgctg aggaccgtct gcggacacat ccagccgcac cagatccttc cgaacaccgt
     2521 tgtcgtaaac agggtaatga aggtcaggaa caggagcgcg atcaggacga tgaaaaaatc
     2581 ggtcatccat atctcggcgc cgaaaaaaag gagccgcagc gacgggacat cgaatctgaa
     2641 tgcgctctct cctccaatcc gcaggaaggg ggttacgatg agtatgatga gcagggccgc
     2701 cccggtgatc cgcctgatcg tgtgaaactt cctaatcatc atcatccagc atcttgtgct
     2761 tggcttcttc gactttgttg cgccgtttcc cggaatagta gtgggcgatg atgatcacga
     2821 agagaacaac aagagcgagc ccgaacaaga aatatgccca tgcctgtttt tccatacggt
     2881 tccttacgaa cgcttgccca gtttggtcgc tggtttttcc ttgccgaccg tgaagatgag
     2941 cacaaagatg ccgatcacgg ccgcggtggt gaatccgatc gtggctacaa tggatatgtc
     3001 catgagacac ctcgcaaatt attataatca gccatcagcg atcagctttc agcaaaaagc
     3061 cgttcgctga ccgctgacag cagatagctg aaagctcagc ggccgaaggc cgcgctacgg
     3121 tttcagcgac tccgtatacg ccttatcctg ggaccagccg ctgacggcgg gagtgtacgc
     3181 cgccgtatag tagatgcccc agagaatcag gccccagaaa aggatcagcc atccgacagg
     3241 aagtttcttc gccgaatctt tcacctcaaa gccgggaaaa ttatcgtatt cgtcagccat
     3301 tgtcgactcc ttattttttc ccctgaaggg acctgatgta ggataccagc gcccatatct
     3361 tgttcttctc gatcgcgttc gcgaaggagg gcataccgcc ctttgccgtc ctgccgtcct
     3421 cgaccatgcc ggcctgcgtg ccgttattga tgagctcgaa atagtcgtcg tcgggcatgt
     3481 cgccgctctc atacaggaaa acatcgtcga tcaggctggg gccgatgcct ccttggccct
     3541 tttctccgtg gcatgctgca cagtgctgtt tgtaaagcgc cgctccctgg ttgatggcga
     3601 cagggtcccc tgcgagcggg ttcggaaaat gtttttcctt tatgacaggc ggcgtggccg
     3661 ccgcggcggt tctgggaacc gcggtcccga tcgcctggat ataggcaatc agcgcgtcca
     3721 gttcagtctt gcccttgagg gcagcgatgt cgtcggacgc gtagggcaga ctgttcgctt
     3781 tcatgtgcga agcaatgtcc tgcgggtcga gggtgttgct cttgagccac ccgtacgccg
     3841 gcatgttgga ctcggcgtac atcgcttgcg gattgccgaa gtggatctgg tgccactggt
     3901 ccggatattt gccgccgatg cgcgccaggt ccgggccggt gcgcttcgaa ccccagagga
     3961 aaggccggtc gtaggcgaat tcgcccgcct tggaatattc gccgtagcgc atgacttcgg
     4021 tcttgagcgg ccgcaccgtc tgagtgtggc agttgttgca gccctcccgc tggtagatgt
     4081 ccttgcctgc gagctggagc ggagtatagg gcttgaggtt tgccagcttc gggtgcatgt
     4141 cgtccatcag catcgggagg aacatggtca ccgcggttcc gatgagaatg acgacggctg
     4201 cgacaatcgc gaacagaatc ggcttgttct caagataatg ggccatggta tccccctatg
     4261 ccttcgccgg cgcaagctca agcttcccct tgcgcatggt catgaagatg ttgtagatga
     4321 a
//
LOCUS       DASSLD010000049         2960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1298361, whole genome shotgun sequence.
ACCESSION   DASSLD010000049 DASSLD010000000
VERSION     DASSLD010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298361"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..603
                     /locus_tag="VFK23_00905"
     CDS             181..603
                     /locus_tag="VFK23_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET7317672.1"
                     /translation="MIEIIAGIGVAIFIVYAIFSIVYMMSMKRMNDALHDFILRTEGN
                     ANATLAELKVTLENIRKITDDFSAVSQDARQISNSLVSVEKGMSLLFEYLKERVSSTA
                     EASYAGLKAGIKAGFSTFGKKPTENPTEKGRDDHEGEP"
     gene            587..874
                     /locus_tag="VFK23_00910"
     CDS             587..874
                     /locus_tag="VFK23_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET7317673.1"
                     /translation="MKENLENEKRGGSILVPFLVGAFAGAIAALLLAPKAGKELRKDI
                     KDFAANAKDNLASAIDTGKGYYEGSVSAVKTAIDAGKSAYVEERDKHRHAA"
     gene            complement(1025..2500)
                     /locus_tag="VFK23_00915"
     CDS             complement(1025..2500)
                     /locus_tag="VFK23_00915"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HET7317674.1"
                     /translation="MNPLIFKKTVLLTPPSNDDRRAHDQRVRDALGMGSLGIPLSIMK
                     RLPRFAGKDAPVSCIIGRTGDGFRLIDIGEERSYSLALDLGTTNLVALLYDNIAQKDV
                     LTRSIENPQIAFGSDILSRLHHAMSNQAGEVYRALIDGVNSLVAAVSAEAGIERHHIH
                     AVAAAGNTVMTHFFLGLDVSSIPVDPFVPVVRTPGFFTASELFLDINPEATVYVFPNA
                     GSYVGGDIAAGILASGMHGSDDVSILIDVGTNAEVVIGNRDWMIVGAGAAGPALEEGI
                     SRIGRRAKRGIVYDFEIRGRSVSCKTFDEGMPEGICGSGMISLLYELYKAGMVDKSGA
                     LVPGEYVSETEGERTFTISCENSRDALSITQAEIHNFMKSKAAMFTLLLVLTRSVGIS
                     FGDITNVYVSGALGTGIDPVKAAGIGMLPAWPRGIVKPLGNSSLSGARMLLSDADLVG
                     AVNDVIGKITYKHMHDDPEFMKEFLGAVFIPHTNPDLLKVG"
     gene            2649..>2960
                     /locus_tag="VFK23_00920"
     CDS             2649..>2960
                     /locus_tag="VFK23_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET7317675.1"
                     /translation="MYSTANITRNTLAMVLAGGKGERLSPITIGRPKPAVPFGGKYKI
                     VDFVLSNLFNSGIRKVYILTQYQAYSLNKHIKESWAKWAGLGEFYDTISPETTHIGEQ
                     WY"
BASE COUNT          691 a          829 c          829 g          611 t
ORIGIN      
        1 caatatgccg cactgcactg aatttttcag gtcaaagctt gtaccgccat attcccgcac
       61 tcgaaaggaa tataccttgc atatttccct gaaaaaggat agtttttaca cgaaaccggg
      121 tctgttcaca cctgcattcc gggggcgcac ctatgctata cttaatccaa aggaggttct
      181 atgattgaaa ttatcgcagg catcggcgtg gcaatattta tcgtatacgc cattttttcc
      241 attgtctata tgatgagtat gaaaagaatg aacgatgcac tgcatgattt tatcctgcgg
      301 acagaaggca atgcaaacgc tacgctggcg gaactcaaag tgacacttga gaacatccgg
      361 aaaataaccg acgatttcag cgcagtatcg caggatgcgc gtcagatatc gaattccctg
      421 gtgtccgtgg aaaaaggcat gagtttgctt tttgaatact tgaaggaacg tgtcagctca
      481 actgccgaag caagctacgc ggggttgaag gccggcatca aggccggatt ttccactttc
      541 gggaaaaaac caacagagaa tccaacagag aaagggaggg atgatcatga aggagaacct
      601 tgaaaatgaa aaaaggggag gctccatcct ggtgccgttt ctagtgggag ctttcgcggg
      661 ggccattgcg gcgcttttac tggcgccgaa ggcgggaaag gaactgagga aagacatcaa
      721 ggatttcgcc gcaaacgcga aggataacct tgcctcggcc atcgacacgg ggaaaggcta
      781 ctatgaagga agcgtatcgg cggtcaagac cgctatcgat gccggcaaat cggcgtatgt
      841 cgaggaacgg gacaaacatc gtcatgcggc atgatcgact tgtaatgcaa agcgcgctct
      901 gcggtcggga gcgcgctttt cttgtttaaa aaccgttacc cggttcaaga ccttaccgtg
      961 ctccgcatct tgtcaatcac cggcccttca ttccgcattc cgaaatccgc attccgcatt
     1021 catttcatcc cactttcagc aaatccggat ttgtgtgcgg gataaacacc gctcccagga
     1081 actccttcat gaattccgga tcatcgtgca tgtgcttgta ggtgatcttc ccgataacgt
     1141 catttacggc gcccacgagg tcagcatcgg aaagcagcat gcgggcccct gagagagacg
     1201 aattgccgag gggttttacg atcccgcgcg gccaggcagg cagcatgccg ataccggccg
     1261 ccttcaccgg gtcaatgccc gttccaaggg cgccggagac atatacgttt gtaatgtcgc
     1321 cgaatgaaat gccgacagac cgcgtgagca ccagaagaag cgtgaacatg gccgccttgg
     1381 atttcatgaa attgtgtatc tccgcctggg tgatggacag cgcatcgcgg ctgttctcac
     1441 aggagatggt gaaggtccgc tcgccttcgg tctctgaaac atactcgccg ggcacgagcg
     1501 cgccgctctt gtcgaccatg cccgccttgt agagttcgta gaggaggctt atcatgccgg
     1561 aaccgcagat cccctccggc attccctcat caaaggtctt gcaggagacg ctccggcccc
     1621 tgatctcgaa atcataaacg attcccctct tcgcccgcct gccgatccgg gagatcccct
     1681 cttcgagcgc gggcccggcg gccccggccc cgacgatcat ccagtcccga ttgccgatca
     1741 cgacttccgc gttggtgccc acatcgatca ggatcgagac atcatccgac ccgtgcatgc
     1801 ccgaagccag aatgccggca gcgatgtcgc ctcccacgta gctcccggcg ttcggaaaca
     1861 catagacggt ggcctcgggg ttgatatcga ggaacagctc cgaagccgta aaaaatcccg
     1921 gcgtccggac cacgggtacg aagggatcga cggggatcga gctgacgtcg aggccaagga
     1981 agaaatgggt catgaccgta ttgccggctg cggctactgc atggatgtga tgccgctcga
     2041 tccccgcctc ggcgctgacg gcggcgacga ggctgttgac gccgtcgatg agcgcgcggt
     2101 atacctcgcc ggcctgattc gacatggcgt ggtgcaggcg tgacaggata tcgctcccga
     2161 acgcgatctg ggggttctcg atgcttcgcg tgagcacgtc tttctgcgcg atattgtcgt
     2221 aaagcagcgc gacgagattg gtcgttccca ggtcgagcgc gagggaatac gacctctctt
     2281 cgccgatatc gataagacgg aagccgtccc cggtcctgcc gatgatgcac gaaacggggg
     2341 catccttgcc cgcgaaacgg ggcagccgct tcatgatcga aaggggaatc cccaacgagc
     2401 ccatgccgag ggcgtcccgg acacgctggt catgggcgcg ccgatcgtcg ttcgacgggg
     2461 gtgtcaggag aacggttttt ttgaagataa gggggttcat aggaggactg catagcacat
     2521 ggcgcattga gcatagcgta agcgacttta acgctatgcg ctttgcgctc tgctctttgc
     2581 gattttcgcc gagattgcag actgccagaa aatacactat acttgatgaa aaagcaagga
     2641 gggcctgcat gtattcgacc gccaacataa cgcgaaacac cctcgccatg gtcctagccg
     2701 gcggcaaggg ggagcggctc tcccccatca ccatcggcag acccaagccg gcggtcccct
     2761 tcggcggcaa gtacaagatc gtcgacttcg tcctttcgaa cctgttcaac tccggcatac
     2821 ggaaagtcta catcctgacc cagtaccagg cctactcgct caacaagcac atcaaggaat
     2881 cctgggccaa gtgggcgggt cttggcgagt tctacgacac catctctccc gagacgaccc
     2941 atatcggcga gcagtggtac
//
LOCUS       DASSLD010000050         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1624371, whole genome shotgun sequence.
ACCESSION   DASSLD010000050 DASSLD010000000
VERSION     DASSLD010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624371"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..618
                     /locus_tag="VFK23_00925"
     CDS             91..618
                     /locus_tag="VFK23_00925"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317676.1"
                     /translation="MFSNSTLQWIKEQEDVMKRVHHCLKRGGRIAIQAPARNFCLEFA
                     MYTADTIEHLGLERYYSSWASPWRFPGKEEFEALLSGIGFEAVKVFNRDYRLKFADTS
                     AVLAWWASAGLRPYLEPLQDDDQLRFKAVFAERFEKNRTGRGIEFGFRRLFAFAGKEV
                     RHAGRSRGSGHFDEG"
     gene            575..1474
                     /gene="yedA"
                     /locus_tag="VFK23_00930"
     CDS             575..1474
                     /gene="yedA"
                     /locus_tag="VFK23_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013702940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HET7317677.1"
                     /translation="MPDGAAAQGILMRDEGKLKVLIALATLYLAWSSTYLAIRISLEG
                     FPPFFVAGSRYMLVGLGMYLYLQYKRVSVPTMAQCLGSLAVGTFLLLGGTGGVVYAEQ
                     WVGSGLAAMVLATTPLWTVLFAGIWKQWPRRYEWIGLALGIAGIFLLNLEGDLRAHPA
                     GAALLVLSAASWSFGSVLSLRVPLPSGMMASAVQMLSGGIVVLLVGFVSGERITSMPS
                     LRAVSAMIYLAVFGSLLGYTAYTYLLKTVRPSLATSYAYVNPVLAVLLGVWLGGEKIS
                     MTEVAAMPIILAGVVLVIIGQKK"
     gene            1509..2381
                     /locus_tag="VFK23_00935"
     CDS             1509..2381
                     /locus_tag="VFK23_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7317678.1"
                     /translation="MQMTKNVHALKIPFQVPISPGKAVERFVYVYLIYGERICLIDTG
                     VATSEKVIFEYLKKTGRRAEDISQLLLTHSHPDHIGAARAVQETSGCKVASHDAERDW
                     IEDIDLQAKERPVPGFYSLVGGPIRIDRILNDGDSLSLEPGLTLDVIHTPGHSRGSLS
                     FFLREEGALICGDAIPIAGQMPIYDDVADAISSIKKLECINGVKSLLSSWDEPRMGNS
                     SIEVMARGIRYVKRIHEAAIKAAGNDPAPDAMAVCRAVLQELGLPQAMANPLVARSIE
                     AHLRVRGSLGGGIQ"
     gene            2417..2719
                     /locus_tag="VFK23_00940"
     CDS             2417..2719
                     /locus_tag="VFK23_00940"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HET7317679.1"
                     /translation="MEKDSVYVLLDVTIMDKAKFVEYVEGHKASMQQYGGRLLFRSND
                     MQVIEGTWSPKLFVVHEWPNEGAFRQWYSSQEYAPWKKLCREAMDMNVVLARKMPG"
     gene            2750..>3011
                     /locus_tag="VFK23_00945"
     CDS             2750..>3011
                     /locus_tag="VFK23_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317680.1"
                     /translation="MIVFDRPGPANTDRVIDIASAASSRCNVIVVASVTGESAIKTAQ
                     KVKDKKIVCVTCPQGMYWEVDKMDSGPFAEIPELKSIRDAWKA"
BASE COUNT          648 a          756 c          947 g          660 t
ORIGIN      
        1 aaagcgaaag aggggtctac ggacatgaag aacgtgcgct acgtgcaggc ctcagcacaa
       61 gtcatgagct ttaaagagta attcgacctc gtgttctcaa actcgacgct gcagtggatc
      121 aaagagcagg aagacgtgat gaagcgcgtc caccactgcc tgaagcgggg agggcggatc
      181 gccatccagg cccctgctcg gaatttctgc ctggagtttg ccatgtatac agccgacaca
      241 atcgagcatc tcgggctgga gcgatattac tcgtcatggg cgtcgccgtg gcggtttccg
      301 gggaaagaag agttcgaagc acttctctcc ggcatcggat ttgaggcggt gaaggtattt
      361 aaccgcgatt accgacttaa gttcgccgac acgagcgcag tgctcgcctg gtgggcgtct
      421 gcgggcttgc gaccgtacct ggagccgctt caggacgatg atcaactgcg attcaaggcg
      481 gtcttcgccg aacggtttga aaagaaccgg accgggcgcg gcatcgaatt cgggttccga
      541 cggctttttg ccttcgcggg aaaagaggta cgacatgccg gacggagccg cggctcaggg
      601 cattttgatg agggatgaag gaaaactgaa agtccttatt gccctcgcga ccctctacct
      661 tgcctggagt tcgacatacc tggcgattcg gatttcgctg gagggctttc cgcctttttt
      721 cgttgcggga tcgaggtata tgctggttgg attgggcatg tatctgtacc tgcaatataa
      781 acgcgtttcc gtccctacga tggcacaatg tctcggctcg cttgcggtag gcacgttcct
      841 ccttctgggt ggaaccggcg gggtcgtgta cgccgagcag tgggtggggt ccggtctggc
      901 tgcgatggtg ctcgccacga cgccgctctg gaccgtgctg ttcgcgggca tctggaaaca
      961 gtggccgcgc agatacgagt ggatcggcct tgccctcggc attgccggga tatttttact
     1021 caaccttgag ggagacttgc gggcccatcc cgcgggggct gcgctcctgg tcctttcagc
     1081 ggcgtcgtgg tcgttcggat cggtattgag cctgcgggtg ccgcttccct cgggcatgat
     1141 ggcgagcgcc gtgcagatgc tttccggagg tatcgtcgtt ctgctggttg gctttgtatc
     1201 gggcgagcgg atcacgtcga tgccctcgct gagggctgtc agtgcgatga tctatctcgc
     1261 ggtcttcgga tcgctcctgg gttacacggc ctatacttat ttgctgaaga ccgtgcgccc
     1321 ctcgctggca accagttatg cgtacgtaaa ccctgtgctc gccgtccttc tcggcgtctg
     1381 gctcggcgga gagaaaatat cgatgacgga ggtcgcggcg atgccgataa tccttgcagg
     1441 ggttgtactc gtaattatag ggcagaagaa gtgaccattc cggattgcga gggaagaggg
     1501 aaacaggcat gcagatgacg aaaaacgttc atgcgcttaa aataccgttt caggtgccga
     1561 tcagccccgg aaaagcggtc gagcgattcg tgtacgtcta tctcatctat ggcgaacgga
     1621 tatgcctcat cgacaccggg gttgctacat cggagaaggt catctttgag tatctcaaaa
     1681 aaaccggcag gcgggcggag gatatatcgc agctgctcct gacgcactcg catcccgatc
     1741 atatcggcgc tgcccgggcg gttcaagaga catcgggctg caaggtagca tctcatgacg
     1801 ccgagcgaga ctggatcgag gatatagacc tccaggcgaa ggaacgtcct gtgcccggat
     1861 tctattcgct cgtcggtggc cctatccgga tcgaccgtat cctgaacgat ggagactctc
     1921 tttcgcttga acccggcctg accctcgacg tcatccatac tcccggccat tcccgggggt
     1981 cgctgtcgtt ctttctccgg gaagaagggg cgcttatctg cggtgacgct attccgattg
     2041 cggggcagat gccgatctat gatgatgttg cggacgcaat cagctctatc aaaaaactgg
     2101 agtgcataaa cggcgtaaag tcccttttgt cttcgtggga cgagccacgt atgggaaatt
     2161 cttcgatcga ggttatggca agaggcatcc ggtatgtaaa gagaattcat gaggcggcga
     2221 tcaaggccgc cggtaatgat ccggcgccgg acgcgatggc tgtctgtcgg gctgtcctgc
     2281 aggagcttgg gctgccgcag gcaatggcca atccgctcgt cgcacggtct atcgaagcgc
     2341 atttacgagt gcgcggaagt cttggtggcg gaatacaatg atgcagctca aacatcgcta
     2401 aaggagcgcc ggcagaatgg aaaaggacag cgtctacgtg ctgctcgatg taacgatcat
     2461 ggacaaggcc aaattcgtgg agtacgtgga aggccacaag gcgtcaatgc aacaatatgg
     2521 cggcaggctg ctgtttcgga gcaacgatat gcaggtcatc gagggaacct ggtcgccgaa
     2581 gctgttcgtc gtgcacgaat ggccgaatga aggagcattc agacagtggt attcttcgca
     2641 ggagtatgcg ccctggaaga agctgtgcag ggaagcgatg gatatgaacg tggttcttgc
     2701 gaggaaaatg ccgggttagt tctgacatca ggaaggggga aacggataga tgattgtttt
     2761 cgacaggccg ggaccggcga atacggacag ggtcattgat atagcgagtg cagcgtcctc
     2821 cagatgcaac gtcattgtag tggcgtccgt tacgggagaa tccgcaataa agaccgcgca
     2881 aaaggtgaag gacaagaaaa tcgtctgcgt tacgtgtcct cagggaatgt attgggaagt
     2941 agacaagatg gactccgggc cgttcgccga aatacctgaa ctgaagtcga tcagagatgc
     3001 ctggaaagca g
//
LOCUS       DASSLD010000051         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_646389,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000051 DASSLD010000000
VERSION     DASSLD010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646389"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1647
                     /locus_tag="VFK23_00950"
     CDS             <1..1647
                     /locus_tag="VFK23_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7317681.1"
                     /translation="STIAEAGEAMTRFSVNVLPVLHNGDYEGIITREVVQKALFHGLG
                     EQNVGEFMTTGGATADPDMPMSQVERVMIEEHQRFVPVIDREGRLVGAITRTDLLRSL
                     HEERLAEVPGLDETAHRSARSARGLLEERVPQEVREALHLVGEVADEIGFPVYLVGGI
                     VRDLFLRVTNTDIDIVVEGDGITFAGMLVKKAGGRMKTHQKFGTAVVVLPSGLKLDIA
                     TARLEYYESPAALPTVELSSIKKDLYRRDFTVNTLAVRLNRARYGELLDFFGGLRDIK
                     DKTIRVLHSLSFVEDPTRVLRAIRFEQRFGFHLSKHTQNLIKSAVNMKLFNRLTGERI
                     YTELVLMFSEAEPQKVLRRMKDFDLLKFIHSELKGSADLDRLFGAIAETLTWFRLLYL
                     DLKVEKWFVYFLGLFDRMKDASAAEALERLAVPLRVRQRMQQARTRSRDVLYLFYKEQ
                     KLPRSRINDLLAPLDTEAILLMMAKARQERAKKYISLYLTHLRNVKVTLTGDDLKKAG
                     IPPGPRYREILAELLDAKLDGLVGTHEDELEFVKGAGKKA"
     gene            1908..>2443
                     /locus_tag="VFK23_00955"
     CDS             1908..>2443
                     /locus_tag="VFK23_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317682.1"
                     /translation="MSKASICVLLCVVMLGAWRPAYADTAAALTEVYGDNGLPEWGRP
                     VQKGLHGLIGAGLFRGDRLVGDPRTRTALLPVVLMTWDDLAYWSITGGGVWLLQFVDV
                     PVRMGAGVKVHPGYTPGDDPDLAGMQKRKSSFDGYVNALWKTELVNIGAAYYHDIARV
                     TRGDAVTVRFSRHIPLTS"
BASE COUNT          474 a          741 c          766 g          462 t
ORIGIN      
        1 agcaccatcg ccgaggcggg cgaagccatg acccggttca gcgtgaacgt gctgccagtc
       61 ctgcataacg gcgactacga agggatcatc acgcgtgagg tcgtgcagaa ggccctcttc
      121 cacggcctcg gcgaacagaa cgtgggggaa ttcatgacca cgggcggcgc gaccgccgac
      181 cccgacatgc ccatgtccca ggtggagcgc gtcatgatcg aggagcacca gcgcttcgta
      241 cccgtgatcg accgggaagg gaggctcgtg ggggcgatca cccggaccga ccttctgcgt
      301 tcgctccatg aggaacggct ggcggaggtc cccggccttg atgagaccgc gcaccgttcc
      361 gccaggtccg ccagagggct gctcgaggag cgtgtgccgc aagaggttcg ggaagcgctc
      421 catctcgtcg gcgaggttgc ggacgaaatc ggcttccccg tctatctcgt gggggggatc
      481 gtccgcgacc tcttcctgcg agttacgaac acggacatcg acatcgtggt cgagggagac
      541 ggcattacct tcgccggcat gctggtcaag aaggcaggcg gccgcatgaa gacccaccag
      601 aagttcggca ccgccgtggt ggtgctgccc tcgggcctga aactcgacat cgccacggcc
      661 aggctcgagt attacgagtc cccggcggcc ctgcccacgg tggagctttc gtccatcaag
      721 aaggacctct accggcggga cttcacggtc aacacgctcg cggtgcggct gaaccgggcg
      781 cggtacggag aactcctcga ctttttcggg ggcctccggg acatcaagga caagaccatc
      841 cgggtcctgc acagcctctc cttcgtcgag gacccgaccc gcgtgctgcg ggcaatccgc
      901 ttcgaacagc gcttcggctt ccatctgagc aagcacaccc agaacctgat caagtcggcc
      961 gtgaacatga agcttttcaa ccgcctgacc ggggagcgga tctatacgga gctcgttctc
     1021 atgttttccg aggccgagcc gcagaaggtc ctccggcgga tgaaggactt cgatctcctg
     1081 aaattcatcc actccgagtt gaagggctcg gcagacctgg accggctctt cggcgcaatc
     1141 gccgagacgc tcacgtggtt caggctgctc tacctcgacc tcaaggtcga gaagtggttc
     1201 gtctattttc tcggcctctt cgacaggatg aaggatgcct cggcggccga agcgctcgag
     1261 cgcctggcgg tccccctgcg tgtcaggcag cgcatgcagc aggcgcgcac gcgttcccgc
     1321 gacgtcctct acctgttcta taaggaacag aaactccccc ggagccggat caacgatctc
     1381 ctcgccccgc tcgacaccga ggcgatcctc ctgatgatgg caaaggcgag gcaggagcgg
     1441 gccaagaaat acatctccct gtacctcaca cacctcagga acgtcaaggt cacgctcacg
     1501 ggtgacgacc tcaagaaggc tggaataccg ccggggccgc ggtaccggga gatcctcgcg
     1561 gagctgctcg acgccaagct cgacgggctg gtcgggacgc atgaggacga actcgagttc
     1621 gtgaaagggg cggggaaaaa agcttgagca ggaccgtgct cgcgcgtcgc tcgctaaaaa
     1681 gtcttttaat cgttgtcgcg aggtattagc aggtcactga aaaagtcttg atttgtcatt
     1741 gcggggagcg tttttcgcga cacggcaatc tcgatgctac tgatttcttt aaaaccgaga
     1801 ttgcttcgct tcgctcgcaa agacggctaa aagacttttt cagcaaactg ttagaccgtt
     1861 gcccggtcct ttgatagaga atgtatatcg aaggaggata cgtctctatg tccaaggcat
     1921 cgatatgtgt tctactctgc gtggtcatgc tcggcgcctg gcggcccgca tacgccgaca
     1981 ccgcggcggc cctgaccgag gtctatggcg acaacggtct gccggaatgg gggcggcccg
     2041 ttcagaaggg cctgcacggg cttattggcg cggggttgtt ccggggggac cggctcgtcg
     2101 gcgatccgcg taccaggacg gccttgctcc cggtcgtcct gatgacctgg gacgatttgg
     2161 cctactggag cattaccggt ggcggcgtgt ggctgctgca atttgtagat gtaccagtac
     2221 ggatgggggc gggcgtaaag gtccatcccg gctacactcc gggcgacgac ccggaccttg
     2281 ccggcatgca gaagcgaaag tcctcgttcg acggttatgt gaacgccctc tggaagacgg
     2341 agctcgtcaa catcggcgcc gcgtattatc acgatatagc cagggttacc cgcggtgacg
     2401 cagtcacggt ccggttttcg cgtcatatcc cgttgacgtc cag
//
LOCUS       DASSLD010000052         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1102803, whole genome shotgun sequence.
ACCESSION   DASSLD010000052 DASSLD010000000
VERSION     DASSLD010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102803"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(411..486)
                     /locus_tag="VFK23_00960"
     tRNA            complement(411..486)
                     /locus_tag="VFK23_00960"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Asn,seq:gtt)
     gene            complement(639..1232)
                     /locus_tag="VFK23_00965"
     CDS             complement(639..1232)
                     /locus_tag="VFK23_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HET7317683.1"
                     /translation="MSFINYSSREINCKIVYYGPGLCGKTTNLQYIYKKTNPESKGKM
                     ISLATETERTLFFDFLPLALGEIRGFKTRFHLYTVPGQVFYDASRKLILKGVDGVVFV
                     ADSQIERMEANIESVENLRLNLSEQGYDLDRVPYVIQYNKRDLPNVVPVEEMRRALNP
                     RNAPDFEACAADGTGVFDTLKAIAKKVIMELKKGREG"
     gene            complement(1286..1780)
                     /locus_tag="VFK23_00970"
     CDS             complement(1286..1780)
                     /locus_tag="VFK23_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET7317684.1"
                     /translation="MLGSDLVMYEEEFKQIDTELQRLLQQANAKVVFLVDKNGQLIAA
                     TGETENLDTTSLASLTAGNIAATGGMAKLLGEKEFSILFHEGARDNIHISIISQRVIL
                     VVIFDQRSSLGLVRLRVKKSAETLGSIFNNLLQKVEMEKGEGKGSESPFAEITDEDID
                     KLFG"
     gene            complement(1953..>2645)
                     /locus_tag="VFK23_00975"
     CDS             complement(1953..>2645)
                     /locus_tag="VFK23_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7317685.1"
                     /translation="IETMYRALRKKGKIKKDIKFIAFGGDGGTYDIGFQALSGAMERG
                     HQLVYICYDNGAYMNTGIQRSSATPFGADTTTQPVGSKVAGKTQRRKDLTRIMAAHNV
                     AYAAQASPHNWKDLITKVKKALATNGPAYMGVVSPCNRGWRSQTNDSILLSRLAVQTC
                     FWPLYEVQDGKWTINYDPKDKKKPVAEWLKPQGRFKHLFAPGNEALLEQFQKFVDDDW
                     ASLKKLQEMSKS"
BASE COUNT          552 a          712 c          714 g          667 t
ORIGIN      
        1 tttactctgt cactcataag gaatagtctc cctacaagaa agaaacaaag gccgtaaccc
       61 ttgcacgcat ctggggttac ggccgtaatt atttccggtc tgaaaagccc tgcaatgcca
      121 ttgctcctcc tccacgaaag agtgccgact gcaatggact gctttccagc aactactcaa
      181 actaatttgt aaagcttact gaagaaggcg ggtaaataat gatattaaat cttcgcagag
      241 aggatgataa aagtcaatcg ccaaagcagg gtattttttg ggtaggcact aaggaacaat
      301 atttgttgga taaaccaaac atgacagtga cttagcgagt atgttttgtt gagcgtcgca
      361 catcaataaa aaagcctgat tttaatataa taaaatcagg ccattaagtc tggctccccg
      421 ggccggactc gaaccagcga cagggtggtt aacagccacc tgctctaccg actgagctac
      481 cggggaatat gtcggcattt ctttataacg tgtaaatcag agacgtataa ataccatata
      541 gcgcgagtta agtcaacgga ttttctgact tttaattcgg cattccgaaa cctgccccgg
      601 caatttacaa gccggggtcc gcttccgcga ttttcttcct acccttcccg ccccttcttc
      661 agttccatga tcaccttttt cgcgatcgcc ttcaacgtat cgaacactcc ggtgccgtct
      721 gcggcgcatg cctcgaaatc gggagcattc cgcgggttga gcgcccttcg catctcttcg
      781 acgggcacca cgttcggcag atccctcttg ttgtactgga tgacgtaggg aaccctgtcg
      841 aggtcgtacc cttgctccga aagattgagg cgaaggtttt cgacgctctc aatattggct
      901 tccatgcgct cgatctgcga gtcggcgacg aacacgacgc cgtccacgcc cttgaggatg
      961 agtttccggc tcgcgtcata gaacacctgc ccgggcacgg tgtagagatg gaacctcgtc
     1021 ttaaaacccc gtatctctcc cagggcgagg gggaggaagt cgaaaaacag cgtgcgttcg
     1081 gtctccgtgg cgagcgagat catcttgccc ttgctttcgg gattcgtctt tttgtagata
     1141 tactggagat tcgtcgtctt gccgcagagg cccgggccat agtagacgat cttgcaattt
     1201 atttctcgcg acgaataatt gatgaaggac atggggccga acttcctctc cttgcgtgta
     1261 taacagggga tactgcgagg ctgggctatc cgaagagctt gtcgatatct tcgtcggtta
     1321 tctctgcaaa gggggactcg ctgcctttac cctctccctt ttccatttcc actttctgca
     1381 gcaggttgtt gaagatgctg ccgagcgtct cggcgctctt tttcacccgg agccgcacga
     1441 ggcccagcga tgagcgctgg tcgaaaatga ctaccaggat cacccgctgg ctgatgatgg
     1501 agatgtgaat gttgtcccgt gcgccctcgt gaaagaggat ggagaattcc ttctctccca
     1561 ggagcttggc cataccgccg gtggcggcga tgtttcccgc cgtcagagat gcaagagaag
     1621 tcgtatcgag gttttccgtc tcacccgttg cggcgatgag ctgcccgttt ttgtccacaa
     1681 ggaagacgac cttcgcgttc gcctgctgaa gaagacgctg aagttctgtg tcgatctgct
     1741 tgaattcttc ttcatacatg accaagtcgg aaccaagcat gtctgtctcc ctgttcggaa
     1801 tatgccccac gtggccgccg acggtgcgca gtcggagcta ttaaaccata ctgaccatga
     1861 tatttcaaga ctttttttgt ttctccggaa atgaaaatag ccggggagaa tctccccggc
     1921 tatccgtggc gcatgccgat gtcggcaaac cgtcagctct ttgacatctc ctggagcttc
     1981 ttgaggctcg cccagtcatc atcgacgaat ttctggaatt gttcgaggag cgcttcattg
     2041 cccggggcaa aaaggtgctt gaaccggccc tggggtttga gccattcggc aaccggcttt
     2101 ttcttgtcct tcgggtcgta attgatggtc cacttgccgt cctggacctc gtagagcggc
     2161 cagaagcagg tttggacagc gagccgtgag agcagtatcg agtcgttggt ctggctccgc
     2221 cagccgcggt tgcagggaga aacgacgccc atgtacgcgg gtccgttcgt ggccagggcc
     2281 ttctttacct tggttatgag gtccttccag ttgtgcgggg aggcctgtgc cgcgtatgcc
     2341 acgttgtgcg ccgccatgat acgggtgagg tccttgcggc gttgcgtctt gcccgctact
     2401 ttcgagccca caggctgggt ggtcgtgtcc gcaccgaagg gcgtggcgct cgagcgctgg
     2461 atgccggtgt tcatgtacgc gccgttatcg tagcaaatgt agacgagctg gtggccgcgc
     2521 tccatggcgc cggaaagggc ctgaaacccg atgtcgtagg tcccgccgtc gccgccgaag
     2581 gcaatgaact ttatgtcctt cttgatcttc ccttttttcc ggagcgcccg gtacatggtc
     2641 tcgat
//
LOCUS       DASSLD010000053         4730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1591985, whole genome shotgun sequence.
ACCESSION   DASSLD010000053 DASSLD010000000
VERSION     DASSLD010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591985"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(605..1381)
                     /locus_tag="VFK23_00980"
     CDS             complement(605..1381)
                     /locus_tag="VFK23_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET7317686.1"
                     /translation="MKSPRTSKTVRSMIALTLLAAGVFLLQSVGDRAYAQPQAADLVK
                     ADVGKVLIDPADTVILLLDHQAGLFQTVKDISVAELRTNTIILAKVAHMMKIPVITTA
                     SVPTGPNGPLMPEIHQNAPDAIYVPRTGEISAWDNKQFVETVRKTGRKTLIIAGVWTS
                     VCVAFPALQAKAEGYKVYTVMDASGDPSEMASRTTLARMTQAGIVPVSTNAVFCELQR
                     TWTRPDAAAFGALYGLYAPNYQAVAESFQKAQEALKPEKK"
     gene            complement(1631..1789)
                     /locus_tag="VFK23_00985"
     CDS             complement(1631..1789)
                     /locus_tag="VFK23_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317687.1"
                     /translation="MSYSDDSNSTGPKQTPNPRPDPTGNAGTTGWLVALNSFLERAFL
                     KADVDTGC"
     gene            complement(2260..2394)
                     /locus_tag="VFK23_00990"
     CDS             complement(2260..2394)
                     /locus_tag="VFK23_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317688.1"
                     /translation="MGHKYIKTTMIYVDVARPHIREQVEKLDKISVPPPPQRMKALRL
                     "
     gene            2672..3880
                     /locus_tag="VFK23_00995"
     CDS             2672..3880
                     /locus_tag="VFK23_00995"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HET7317689.1"
                     /translation="MKARLWILLCGLVALMVSGAIPVNAQEHQKGMVVWPDSSVEREE
                     DIGIRAHTNHLILLDPKSGGGTPAGLTPVIIRGAYGFSTYTGLQGSQTIVIVDAYDYP
                     TAVTDFNAFSQVFGLPQETGSGNVLQIVYAKGAKPSYNSGWSQEAALDIEWAHALAPT
                     AKIVLVEAASNSLSDLLYAVDVAGTTIAGAREVSMSWGSGEFSGETLYDSHFTASGVV
                     YFAASGDSGGKTIWPSVSPNVVSAGGTTLNVDSSGNFLSETGWSGSGGGKSRYESRPT
                     YQTVIMNIVGNKRGTPDLSFDADPNTGVSVYWQGGWYVFGGTSVSTPSLAGIVNLAAS
                     SGNGFANNTADELGSRVYLNYSNSSPDPYSGSGDDFRDIISGTVGRFSCSPGWDFVTG
                     IGSDWGLAGK"
     gene            complement(4093..4407)
                     /locus_tag="VFK23_01000"
     CDS             complement(4093..4407)
                     /locus_tag="VFK23_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="panthothenate synthetase"
                     /protein_id="HET7317690.1"
                     /translation="MRLLVNVTLPNEPYNTAIRDGSFSEKLDRILKETRPETVYFTER
                     NGHRGATLIVNLTEPSEIPAIGAPWFLAFNAEVEYRIALTPEDLRKAGLADVGKKWPK
                     AA"
BASE COUNT          937 a         1270 c         1388 g         1135 t
ORIGIN      
        1 tactggcatg cgcgggggcc ggagtttctg cacggcgaaa tcccgcacct catcgaatgg
       61 ctacgcatgc cgggcgacct cattttcatc ggtctgggcg ctttcccgct gctggtcgcg
      121 gcgttcctta cctggtggtc cgagcggagg aaatccgcga ggtgacgaac accattgaac
      181 catcgccggt ggagtcggtc ttcttgtctt cgaaccgtca ggtattccgc aacccatacc
      241 gaatcgcgat gatttgacaa ctatggataa tgaccttctt caccagatac cggaggaagt
      301 ggttcagaga ttgagggggt ggtggggaat cccgtgcggg ttcaaatctc gctttcgaca
      361 ccatctcaat aatcaaaggg aatccggatt aacgccgggt tccctttttt cgcgaaatcg
      421 gaccgagtgg ctaatttgtg gctcgcggtt ctgattctga cgtccggagc cacggactgg
      481 aatatcttcc ccgccagtag gacgttcgtt tccggacttg aaggaccggg aggcaacttg
      541 aagcctactc cccggtcctt cgattctatc tatatttgga tcttcatcgc cttccgtgtt
      601 gctctcactt cttctccggc ttgagggcct cctgcgcttt ctggaagctc tccgctacgg
      661 cttgatagtt cggagcgtac aatccataca gggcgccaaa cgccgcagcg tccggccgag
      721 tccaagtgcg ttgcagctcg caaaaaaccg catttgtgct gaccgggaca attccggcct
      781 gcgtcatgcg ggcgagggtc gttctcgatg ccatctcgct cgggtcgcct gaagcatcca
      841 tcactgtata gactttgtag ccttccgcct ttgcctggag ggccgggaag gcgacacaaa
      901 cgcttgtcca tacgccggcg atgattaatg tctttctgcc ggtcttgcgt acggtctcca
      961 cgaactgctt gttgtcccag gcgctgattt cacccgtgcg cggaacgtag attgcgtcgg
     1021 gtgcattttg gtggatctcc ggcatcaacg ggccattcgg gccggtcggt acggatgcgg
     1081 tcgtgatgac ggggatcttc atcatatggg ccaccttcgc caggatgatg gtattggttc
     1141 gtaattccgc cacactgatg tccttgacgg tctggaacaa gccggcctgg tggtcgagta
     1201 ggaggatgac ggtatccgcg ggatcgatca ggaccttgcc cacgtccgcc ttcaccaagt
     1261 cggcagcctg cggctgagcg taggcacgat cgccgaccga ctgtaataaa aacacacctg
     1321 ctgcgagcag agtgagggca atcatcgacc taacagtttt cgatgtccga ggcgatttca
     1381 tgagtctctc ctttcgacgg tgttgcgaga ccaggttctt gcatcgttcg gccgatcagg
     1441 gctttgcttc ctattgttcg tagaaacaag gatggcccgc caattttcac ctccctttcc
     1501 gccaaggcct tcccacagag accctcttcc aaattacaaa cacggtaatc ctgtccgcct
     1561 acaagcctgc ctacatttac catccggagt tcgagattgg cgggcagacc gcccgcccgg
     1621 tttatggggg tcaacatccg gtatccacat ccgccttaag gaacgcgcgt tccagaaacg
     1681 aattcaacgc gaccagccac ccggtggtgc cggcgttccc tgtcgggtcc gggcgcgggt
     1741 tcggggtctg tttggggccg gtgctgttgc tgtcgtcgct gtagctcatt gactctccgc
     1801 cgtcaggacc atgcctaccc gattcgcctg attctgcaaa tgaacccata ttcccttaag
     1861 atagacatcc ggagggttta gcttcccgta tcggacggtc aaagtattaa ccttgtggga
     1921 caacgtgaga tgggcagggc caggtttatc gattggtctc gctagctatc ccccgcaatc
     1981 cccaaaggga atttggcttc atgccaagtt cctatttttc gtaaaaacgg gtctaggggt
     2041 tcaaatgatt gcagtgggaa ttgggacgat tttaatttaa tttttccggt ttatatgaaa
     2101 ttgatgacgg gatttccgtc ccttcatgcc gaaggcgatg gggcggaata tctccattga
     2161 ttcggttatc tgggagtgga ctgctgccca agtctcctca tcttgagggg gtggtgggga
     2221 accccgtggg ggttcgagtc cgactccagt aacagtttgt tacaagcgca gcgccttcat
     2281 cctctgtggc gggggcggaa ccgatatctt gtcgagcttt tccacctgct cccgtatatg
     2341 gggcctggcg acatccacgt agatcattgt cgtcttgatg tacttgtgac ccatcgcctt
     2401 ctggatggcg cgcccgcacc tcaagccgag cggggcggaa tttctccatt gattcgattt
     2461 cggttattgc ggcttcttcc tgattatggt tgtcctgaaa atcgtccgtc ggagggatcc
     2521 aatccggacc cctccgaatc caaagggaaa agaaccgtat gagagggagg atgtgtactc
     2581 gggtaccttt ccccaaagaa atgtaccgga ggggtccggg aattttcgcg ggcaatatgc
     2641 ctccttatcg aggcaaagaa aggagcgtga tatgaaagct cgattatgga ttttgttgtg
     2701 tggattagtg gcgctaatgg tatcaggggc gatacccgtc aacgctcagg aacatcagaa
     2761 gggcatggtg gtttggccgg actcaagcgt ggagcgtgag gaagatatcg gtataagggc
     2821 gcataccaac caccttattc tcttggatcc gaagtcagga ggagggacac ccgctgggtt
     2881 gaccccagtg atcatcaggg gtgcttatgg tttctcgacc tatacgggac tgcaggggtc
     2941 acagacgatt gtaatcgtgg atgcttatga ttatcccact gcagtgaccg atttcaacgc
     3001 tttctcgcaa gttttcgggc tgccgcaaga gacgggcagt ggcaatgtgc ttcaaatcgt
     3061 atatgcgaaa ggggccaaac ccagctacaa ctccggttgg tctcaagaag ccgcgcttga
     3121 catcgaatgg gctcacgctc tcgcgcccac ggctaagatc gttttggttg aggcggcgtc
     3181 aaacagcctg agcgatctgc tctatgcagt ggatgtggcc ggcaccacta ttgctggagc
     3241 gcgggaggta tcgatgagtt gggggtccgg cgagttttcc ggtgagaccc tctacgacag
     3301 ccacttcaca gcgagtggcg tcgtctactt tgcggcaagc ggcgactccg gggggaagac
     3361 catttggccg agcgtctccc cgaacgtcgt ctccgccggt gggaccacgc tcaatgtgga
     3421 cagctcaggc aacttcctta gcgagacggg ttggagcggc agcggaggcg gcaaaagcag
     3481 atacgagtcg aggcccactt accagaccgt catcatgaac attgtgggaa acaagcgggg
     3541 cacgccggat ctgtcattcg acgccgatcc aaacacgggc gtttcagttt attggcaggg
     3601 cggctggtat gtgttcggcg gtacaagcgt ttctacgccg tccctggcgg gtatagtcaa
     3661 tctcgcggct tcttccggga acggttttgc caacaacacg gcggatgagt taggcagtag
     3721 ggtttacttg aattattcca acagttcacc cgatccctac tcaggctcag gcgatgattt
     3781 tcgcgacatt atctccggga cggtggggcg tttcagctgc agtccgggtt gggatttcgt
     3841 gacgggcatc ggcagcgact ggggattagc cggaaagtag tttgcaccac cgaggtcggc
     3901 ggcggctttg ctcatggccg ccgccggctt gctatatttt ggcctgacgc caagttccct
     3961 tttttcgcga gatcgggccg tgggtctcac atgattgcag ctggaagggg acctggagaa
     4021 ataacggggg ccgcgagcga aacccgcagc ccccgattca cgactcgctg ccgttactcc
     4081 cgcctcggaa tctcaggcgg ccttcggcca cttctttccg acatccgcga gcccggcctt
     4141 tctgaggtct tcgggggtca aggcgatcct atactcaacc tcggcgttga aagcgaggaa
     4201 ccacggcgcc ccaatcgcgg ggatctccga tggctcggtg aggttgacga tgagggtcgc
     4261 ccctctgtga ccgttccgtt ccgtgaaata aactgtttcg ggcctggttt ctttgaggat
     4321 gcggtccagt ttctcgctga aggacccatc cctgatggcg gtgttgtacg gctcgttagg
     4381 cagggtcacg ttcacgagca aacgcatgtg agaaccttcc tttctgtcct ggttagcgac
     4441 ggcgaactat gcgccgccaa ggacgtcgga tcgttgacat tcgaccggtt attcacgctc
     4501 tatggttttt tagattccac ggtccgcgcg acagttcctg ccccggaaga ttctcagcca
     4561 agatatgtgc ttattccctg cttggagcaa gttcaaccgg cattaacctt ggaaagtgtc
     4621 gaagaacggg ggtaacctca ggacattgag gtctaaggtg gcaatggatc cgaaaggggg
     4681 ggcaaatggc aaagagcatg gtgatcgtcg gtgcggggat tgccgggctt
//
LOCUS       DASSLD010000054         2487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_776522,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000054 DASSLD010000000
VERSION     DASSLD010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776522"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            389..706
                     /locus_tag="VFK23_01005"
     CDS             389..706
                     /locus_tag="VFK23_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317691.1"
                     /translation="MSIWTVAALVALLNLPFGYWRASVEKLSRPWFLAVHLPVPFVIA
                     LRILSGLGFHLATLPVMIGAYFIGQLLGGMLNQWAKSHAKSPVTSCLVMDAVSGIRNV
                     IPR"
     gene            complement(693..1109)
                     /locus_tag="VFK23_01010"
     CDS             complement(693..1109)
                     /locus_tag="VFK23_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET7317692.1"
                     /translation="MKSLRKELWFDIPSRRGFVNITREVEECVRQSGVWEGLVLVNAM
                     HITASVFINDDERGLLGDFEDWLEKLAPREPVSQYRHNTGEDNADAHLKRSVMGREVV
                     VAITNGRLDFGPWEQIFYGEFDGRRKKRVLVKVIGE"
     gene            complement(1181..1990)
                     /locus_tag="VFK23_01015"
     CDS             complement(1181..1990)
                     /locus_tag="VFK23_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET7317693.1"
                     /translation="MKRNPYILLLVILASFFLSCQTVPITGREQLSFVPASQILPMSF
                     NQYQDFLSKHKVVDQGEQAALVKRVGSRIEHAVERYFAEHGLSSRLAGYQWEFHLVDD
                     KEVNAWCMPGGKVVVYTGILPVTKDENGLGVVLGHEIAHAVANHGGERMSQSLLVEMG
                     GLTLDAALSQNPSKTNDLFLAAYGLGTQVGILLPYSRTQESEADHLGLIFMAMAGYDP
                     HGAVTFWERMIASQHGKTPPELLSDHPADARRIENIKGLLPEAMQYYRPES"
BASE COUNT          565 a          701 c          624 g          597 t
ORIGIN      
        1 ctgatgcgaa agcagtagag gaaaacgcag tacctgcaat acccagaaga aacaaaagga
       61 cacctaattt ttcgtccatg gaagtatctc ctttcgtcta agaaaattat acctccgggg
      121 ggagagctca gatcgcgcag agtttggcgc tgctgcggcc ctttttgtcc ttgccgaatg
      181 cctctatcgg gaatccagtt ctatcacacc ggaaggaaga gactccagat cccggctgag
      241 atcataccgg aagacggaaa gattcggata attgcagaaa aaagtccact gcagtattaa
      301 cgaatttgtt gcttttttat ctgtgcgggt tataatgagc taagtgtcgg ccctttgcgg
      361 ggccatccat gagagaggaa ttgtaaagat gagtatttgg actgtagcgg ccctggtcgc
      421 cttgctgaac ctgcctttcg gctactggag ggcaagcgtc gaaaaattgt cccggccgtg
      481 gtttctcgcc gtgcacttgc ccgtgccctt tgtcatagcg cttcgcatcc tgtcaggact
      541 cggttttcac cttgccacgc tgcccgtcat gatcggtgcg tactttatcg gacagcttct
      601 gggcgggatg ctcaaccagt gggcgaagag ccatgcaaag tcgccggtaa cctcctgcct
      661 tgtgatggac gccgtgtctg gcatcaggaa cgtcattccc cgataacctt gaccagcacc
      721 cgcttcttcc ttcttccgtc gaattcgccg tagaatatct gctcccaggg cccgaagtcg
      781 agcctgccgt tcgtgattgc gaccacgacc tcgcggccca tgacggagcg cttcaggtgc
      841 gcgtcggcat tgtcctcgcc ggtgttgtgc cggtactgag acacgggttc acggggcgcg
      901 agcttttcga gccagtcttc gaaatcaccc aaaaggccgc gctcgtcgtc gttgataaaa
      961 accgaggccg taatatgcat cgcgttcacc agcacgaggc cttcccagac cccgctctgc
     1021 cgtacgcact cctcgacctc gcgcgtgatg ttgacgaagc ccctgcgcga ggggatgtcg
     1081 aaccagagtt ctttgcggag agatttcatt tcaggctgcc cctcttcggc agatataaat
     1141 tcacactaca gcttctgtgc caatgcatgg acgccacctg ctagctttcc ggcctgtaat
     1201 actgcatcgc ctccggcaga agtcccttga tgttctcgat ccgtcttgca tccgcaggat
     1261 ggtcgctcag taactccggc ggcgttttac cgtgctgtga agcaatcatc cgctcccaga
     1321 aagtcactgc tccgtgcggg tcatacccgg ccatggccat gaatataagt cccaggtgat
     1381 cggcctccga ttcctgcgtt ctgctgtagg gaagcaggat gcccacctgt gtccccaaac
     1441 cataggcagc aagaaaaaga tcgtttgtct tactcggatt ctgcgagagc gcggcatcga
     1501 gggtgaggcc gcccatttcc accaggagtg actggctcat gcgctcgcct ccatgattgg
     1561 ccacagcatg cgctatctca tgcccgagca ccacgcctag tccgttctcg tccttcgtaa
     1621 cgggaagaat tcccgtatag actacaacct tgccgccggg catgcaccag gcgtttactt
     1681 ccttgtcgtc cacaaggtga aattcccatt ggtatcccgc gagtctcgac gagagaccgt
     1741 gttcggcaaa ataccgctcg actgcgtgtt cgatgcggct ccctacgcgc tttaccagcg
     1801 ccgcctgctc gccctgatca accaccttgt gctttgaaag aaaatcctga tactgattga
     1861 agctcatggg aaggatctgg ctggccggaa caaaagaaag ttgttcccgc cccgtgatgg
     1921 gtacagtctg gcaagataaa aaaaatgacg ccaaaataac gagcaggaga atataaggat
     1981 ttctcttcat gataaaaccc gccttaatac tggcattgat gatccaatac catttgcccc
     2041 atgactcgtt tccaatcgtg aaaacttcaa cgcttcagga agaactacgc gccgaaagtt
     2101 taacaaacaa taaagacttt tccgcagccg gctccttatc gaggatgagc attcatccat
     2161 agtttgagaa aaagataagc gacgagtaca gcgagcagcg acactgccaa taccgcaaga
     2221 cgaattttca gcatccgtct attccgcatc atatttcgtt cgcgtaggga ccgcagtttt
     2281 tcgcggcgtg cccgacgcga gttgttcgaa tcgtcactga tatgctcttt catttgagtg
     2341 gtacgcgacc ccgcctggga ctacggctcg ttctattcat tttatgctat tcatcaagcg
     2401 atgcctggag tccaaccgtt aggatagaca tcgtttattt tgacacaaat atatgcctga
     2461 cccggcttca tgtcaatgag ctcgaac
//
LOCUS       DASSLD010000055         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_319850,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000055 DASSLD010000000
VERSION     DASSLD010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319850"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VFK23_01020"
     CDS             <1..696
                     /locus_tag="VFK23_01020"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HET7317694.1"
                     /translation="FVRDGLLQCGFPPCPANYMASNPVWCQPLKVWKRYFSTWVAAPT
                     PDAVLNSVTFFDFRPMFGDYALAEALRDYLISILAGQKVFLGYLANMAIKNTPPIGFF
                     KSFVVEKSGEHKDKLNLKVKGLAPLVDIVRLFALEKGVRETSTLERIEALRDKHTTVG
                     EYAADFEHAFEFIMLLRIHNQYAQISAGEAPDNFINPNRLSNLEKRSIKDAFQLISKV
                     QDEIIERYKSLIW"
     gene            789..1493
                     /locus_tag="VFK23_01025"
     CDS             789..1493
                     /locus_tag="VFK23_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET7317695.1"
                     /translation="MLALFAGKDKKQKRIDEKTPITEARYVVVDTELTGLDEKKDSIV
                     SIGAVRMTGGSIDLGGNFYRLISPRTELSAASVVIHEIMPSEVKAKPGIESVLAAFLE
                     FCGDAVLVGHFISIDLSFLNREMKLIRRAGIQNLTLDTFSIYEWLRKRSKSRDCFATP
                     LGGYRLYDIVKCFDIAVNGAHNAVMDAFITAQLLQRFFPLLAEAGIHDIGDLLRIGTP
                     FKGGDSFGLMNDFSNF"
     gene            1529..1840
                     /locus_tag="VFK23_01030"
     CDS             1529..1840
                     /locus_tag="VFK23_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HET7317696.1"
                     /translation="MAKLKKEDILNDSDFLELSSQKNTISIILTILELVLYFGFIYLV
                     AERKDFLSQKMSMDGAATIGIPIAVGTILLSWVFTGIYIWWANVKYDELVKKVIAKVG
                     G"
     gene            1842..>2112
                     /locus_tag="VFK23_01035"
     CDS             1842..>2112
                     /locus_tag="VFK23_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011792886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HET7317697.1"
                     /translation="MPQTQTIIGQANPVAIGFFVAFVITTLLITWWAAKRTRTTKDFY
                     AAGRSITGFQNGLALAGDYMSAASFLGIAGLVSTKGYDGLIYSVGW"
BASE COUNT          473 a          577 c          619 g          443 t
ORIGIN      
        1 ttcgtccggg acggacttct gcagtgcggc ttcccgccct gtcccgcgaa ttacatggcg
       61 agcaatcccg tgtggtgtca gccgctcaag gtctggaaaa ggtatttctc gacctgggtt
      121 gcggcaccga cgccggacgc ggtgctgaac tccgtgacct ttttcgattt tcggcccatg
      181 ttcggcgact atgccctcgc agaggcgctc cgggactatc tgatctcgat cctcgcgggc
      241 cagaaggtgt ttctgggcta ccttgcgaac atggccatca agaacacgcc tccaatcggg
      301 tttttcaagt ccttcgtggt cgaaaaaagc ggcgagcaca aggataaact gaatctcaag
      361 gtcaaagggc tggcgccgct tgtggacatc gtccggcttt tcgcgctcga gaagggggtc
      421 cgcgagacct cgaccctgga gcggatcgaa gccctccggg acaaacatac gaccgtcggg
      481 gaatacgccg ccgatttcga acatgccttc gagttcatca tgcttctcag gattcacaac
      541 cagtatgcgc agatcagcgc gggcgaggcg cccgataatt tcatcaatcc gaaccggctt
      601 tccaatctcg agaagcggtc gatcaaagat gctttccagc tcatctcgaa agtgcaggat
      661 gagatcatcg agcggtacaa gtcgctgatc tggtgatgga acagttgaga gttcggagca
      721 gggagttggg agagggaacg actctccgaa ctacgaactc ctcactccga gcgattcagg
      781 gttcgcacat gctggcactg tttgcgggca aagataagaa acagaagcgg atcgacgaga
      841 agacgccgat cacggaggcc cgctacgtgg tcgtggacac ggaactgacc ggccttgacg
      901 agaagaagga ctccatcgtg tcgatcggcg cagtccgcat gaccggcggg agcatcgatc
      961 tgggcgggaa cttctaccgg ctcataagtc cgcgcacgga gctctcggcg gcaagcgtgg
     1021 tcatccacga gatcatgccg tcggaagtga aggcgaagcc gggcatcgaa tccgtgcttg
     1081 ccgcctttct ggagttctgc ggcgatgcgg tcctggtagg gcacttcatc tccatcgacc
     1141 tcagttttct gaaccgcgag atgaagctca tccgacgggc tggaatacag aaccttacgc
     1201 tggatacctt cagcatctac gagtggcttc ggaaacggag caagtcccgg gattgtttcg
     1261 cgacgccgct cggcgggtac cggctgtacg acatcgtgaa gtgttttgac atcgccgtga
     1321 acggggcgca caacgcggtt atggatgcgt tcatcacggc gcagctgctg cagcgatttt
     1381 tcccgcttct cgccgaggcg ggcattcacg acatcggaga tcttttgcgg atcggaacgc
     1441 cattcaaggg aggtgatagc ttcggactga tgaacgattt cagcaatttt tagcgcacgt
     1501 cacttacaaa ttacaaggag gatgaaacat ggcaaagtta aagaaggaag atatcttgaa
     1561 tgactcggat ttccttgaac tgtccagcca gaagaacacc atatcgatca tcctgacaat
     1621 cctggaactg gtgctctatt tcgggttcat ctacctcgtc gcggagcgca aggactttct
     1681 ctcgcaaaaa atgagcatgg acggcgcggc gacgatcggg atccccatcg cggtcgggac
     1741 catcctgctg tcatgggtct tcaccggaat ctacatctgg tgggcgaacg tgaagtatga
     1801 cgaactcgtc aaaaaagtca tagccaaggt gggaggataa catgccgcag acacagacaa
     1861 tcatcggaca ggctaacccc gttgcaatcg ggttctttgt cgcgttcgtc atcaccacgc
     1921 tcttgatcac gtggtgggcg gctaaacgga cgaggactac aaaagatttc tacgccgccg
     1981 gcaggagcat caccgggttc cagaacgggc ttgccctcgc aggcgactac atgagcgcgg
     2041 cgtcgttttt gggcatcgca ggcctcgtgt ccaccaaggg ctatgacggc ctcatctact
     2101 ccgtggggtg gc
//
LOCUS       DASSLD010000056         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_417526,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000056 DASSLD010000000
VERSION     DASSLD010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417526"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..299)
                     /locus_tag="VFK23_01040"
     CDS             complement(48..299)
                     /locus_tag="VFK23_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317698.1"
                     /translation="MKKLFRKVDDFLAKAGEAEYIGTDIPSEESGEYTVTLESGMAVP
                     HAEPGALQAIKSSAKGHGEQTVLPDDCEFGDNDLCYVEA"
     gene            574..1797
                     /locus_tag="VFK23_01045"
     CDS             574..1797
                     /locus_tag="VFK23_01045"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7317699.1"
                     /translation="MKKGLTLFLVLCVSAALVWLAVFSWNNLRGIGPALRKPSREILR
                     FIERVEASDSTADAPLKLPPGFSLSLFAKGLGGPRVMVSDPSGTILVSIPSQGRVVAL
                     PDTDHDGVADRIVTVIRGLNRPHGMALRCAPSCKLYVAEEHQVNVYSYDGKNFLAKKE
                     KKIADLPQGSGHSTRTLLFLPGPSEGQLLISVGSSCNVCSEKDSRRAKILIVPAEGGN
                     PGVFASGLRNAVFMAVHPKTKRLWVTEMGRDLLGDNTPPDEINIIDQGRDYGWPFCFG
                     KNVHDDDFDPEGTHVCRQPATVPSHIDIPAHSAPLGLAFFPEEGWPKEFRNNLLVSFH
                     GSWNRSSPTGYKIVRYRLDREGRYLGVKDFVSGWLTADGISLGRPVDIMIRPDGTIFV
                     SDDKAGVIYRIVRKK"
     gene            1858..>2425
                     /locus_tag="VFK23_01050"
     CDS             1858..>2425
                     /locus_tag="VFK23_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HET7317700.1"
                     /translation="MCAAECGKRGRSVLVIDHGERIGSKIRISGGGRSNFTNLNASAE
                     HYLSQNPHFCKSALARFGPRDILSLAGKHGIDWYEKEAGQIFCIKSSRDIIAMLEREC
                     REAGVEMRLKCRVTEIAKDEVFTVHTSGGVFTSGSLVIATGGLSYPAVGASGFGHEVA
                     RRFGLRVTQVRPALVPFVFSGREQKVFQG"
BASE COUNT          485 a          720 c          718 g          502 t
ORIGIN      
        1 ttgtgctgcg gcgagacgtt aaagaaatta tggaggcgaa ttatccgcta tgcttcgaca
       61 tagcataaat cgttgtcgcc gaattcacaa tcatcgggca gtacggtctg ctccccatgt
      121 cctttcgctg agctcttgat cgcctgcaac gcaccgggct ccgcatgggg caccgccatg
      181 cccgactcta gggtgacggt gtattcgccg gattcctcgg aggggatatc agtgccgatg
      241 tactcggctt ccccggcctt tgcaagaaaa tcgtcaacct tccggaagag ctttttcatg
      301 actcgcacct ccttctccta agtctctatc attattatat cacccgttta tcggcgcagg
      361 tgtcgcgcgg cgttggccgg caccgacata tttcctttgg gaaatcaatg agtatggtcc
      421 ctcttctgac cgtatctgtt cccgaccatc gcgtcgggtg aacgaagccg gaggcatcgg
      481 ccgcaactcc gtcttgacat gccgtcactg cgcgttatat gatcgagaca aggtgaaagg
      541 aacggcccac ttttttccgg gagcatgcca acgatgaaaa aaggacttac cctcttcctg
      601 gttctctgcg tgtccgccgc gctggtctgg cttgccgtct tctcctggaa caacctgcgc
      661 gggataggcc ccgcattgag gaagccgtca cgggagatat tacgcttcat cgagcgggtc
      721 gaggcgagcg atagcacagc ggatgctccc ctcaaactcc ccccgggctt ctccctttcc
      781 cttttcgcga agggattggg cggaccgcgg gtaatggtat cggacccctc gggtacgatc
      841 ctggtgagca taccctccca gggcagggtg gtcgccctgc ccgatacgga ccatgacggc
      901 gtagccgacc ggatcgttac cgtgatcagg ggcctgaacc ggccccacgg catggccttg
      961 cggtgcgcac cgtcatgcaa actttacgtt gccgaagaac accaggtgaa cgtctattcc
     1021 tatgacggga agaatttcct ggcgaagaag gagaagaaga tcgccgatct gccgcagggt
     1081 agcggccatt ctacgagaac actgctcttt ttgcccgggc cttccgaagg ccagctcttg
     1141 atctccgtgg gatcatcctg caatgtctgc agcgagaagg actcccgcag ggcgaagatc
     1201 cttatcgtcc ccgcggaagg aggaaatccc ggcgttttcg cctcgggcct caggaacgcg
     1261 gtattcatgg ccgttcatcc gaaaacgaag cggctgtggg tcacggagat gggccgggac
     1321 ctgctgggag acaacacgcc gcccgatgag atcaacatca tcgatcaggg ccgcgattac
     1381 gggtggccgt tctgcttcgg aaagaatgtt catgacgatg atttcgaccc ggaagggacc
     1441 catgtctgcc ggcagccggc cacggtccct tcccatatcg acatccccgc gcactcggcg
     1501 cccctggggc ttgcgttttt ccccgaagag ggctggccga aagagttccg caataatctc
     1561 ctcgtctcct ttcacggctc ctggaacagg agttccccca cgggatacaa gatcgtacga
     1621 taccggctgg accgagaagg ccgctatctg ggggtgaagg atttcgtgag cggctggctt
     1681 acggcggacg gcatttcact cggcaggccc gttgacatca tgatcaggcc tgatggaacg
     1741 atcttcgttt ccgacgacaa ggccggcgtg atctaccgga tcgtcaggaa gaagtaggga
     1801 ggagtcatca agaccggaag gaacgtcatc atcatcggcg ccggcgcctc gggcctcatg
     1861 tgcgcggcgg aatgcggcaa gcgaggccgc tcggtgctcg ttatcgacca cggcgaacgg
     1921 atcggctcca agatacgcat ctccggcggc ggcagaagca atttcacgaa cctgaacgcg
     1981 tcggccgagc actacctttc gcaaaacccg catttctgca agtcggccct ggcccgcttt
     2041 ggtccgcggg acatcctgtc actcgccggg aaacacggta tcgactggta cgaaaaagaa
     2101 gccggccaga ttttctgcat caagagctcc cgcgatatca tcgccatgct cgagagggag
     2161 tgcagggagg ccggggtcga aatgcggctc aagtgccgcg tgacggaaat cgcgaaagac
     2221 gaggtcttca cggtccatac gagcggcggc gtcttcactt ccggttcgct cgtcatcgca
     2281 acgggagggc tctcctatcc cgctgtcggg gcgagcggct ttggccacga ggtggcacgc
     2341 cgtttcgggc tcagggtgac gcaggtccga ccggccctgg tcccctttgt attttcaggc
     2401 agggaacaaa aggtctttca gggac
//
LOCUS       DASSLD010000057         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1526249, whole genome shotgun sequence.
ACCESSION   DASSLD010000057 DASSLD010000000
VERSION     DASSLD010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526249"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /gene="gptM"
                     /locus_tag="VFK23_01055"
     CDS             <1..868
                     /gene="gptM"
                     /locus_tag="VFK23_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="geopeptide radical SAM maturase"
                     /protein_id="HET7317701.1"
                     /translation="LSIDTIIRIAETVRSIAATGHIKFTFSFITNGTLLTPRLVEKLK
                     PLGLRAASITLDGPKPVHDAFRPFRSGKGSFDTIVRNIKEVCSQIDVQLGGNYTRDHY
                     RQFPGLLDYLLDSGLTPADISNVRFDPVVNESREMAPPDFHDGCMSFDEPWLRDAAVF
                     LRAEILKRGFRTQKVEPSVCFVERRNNVVVNYDGALYRCPGLIGRRDYCTGSVKTGSA
                     DYRGTHNLDSWKNEECLSCSYLPLCFGGCRYMKLVRDGNMQGVDCKKAYFDRTLEALV
                     MQDVKYDKSDEL"
     gene            922..1842
                     /locus_tag="VFK23_01060"
     CDS             922..1842
                     /locus_tag="VFK23_01060"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HET7317702.1"
                     /translation="MSILPPLMSNGKLKATLLAATVMACLVLLPCPASAKDVIIIKEA
                     EIKPFRDAIEGFKSSCGCRAEEVAFSDKNLLKRIEKARPSAVFVVGTQAFEKATAITD
                     VPVIYTMVMPSEEAFLPADNVSGVRMDVAPETWLKTMTALFPGVKRIGVLSDPELTGP
                     LVRDAAAAARALGITLVVKSISDPRHAPELLNEFQNEIDLFWMLPDPTVVTSETLDYL
                     MLFSFQNNLPVFSFSKKYVKNGAAAALAIDPFDMGVQAGEQYRTLSKGGKGPLRAYAR
                     TSRLIVNRKVITKMKVQTSGEMLQNAESVE"
     gene            1846..>2292
                     /locus_tag="VFK23_01065"
     CDS             1846..>2292
                     /locus_tag="VFK23_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317703.1"
                     /translation="MDLFPRKSLTFRLLSSFVFIIVIVLTIQTVVYILRAQKRIHGEL
                     VRQGELLIDLLAYSSRIAVFTENTEKLQEDAAGMQTEQDVVLVGFYGSKTSPLYVTSL
                     TDRYRLSSDIDISAAIKPGETLMREETDTVEFIKPVALLVNPDATSL"
BASE COUNT          520 a          698 c          592 g          482 t
ORIGIN      
        1 tttgagcatc gacacgatca tccggattgc cgagaccgtt cggtccattg cagcaacagg
       61 tcatatcaaa tttacctttt cttttatcac gaacggcacc ctcctgacac cccgcctcgt
      121 tgaaaaatta aagcccctgg ggcttcgggc tgcaagcatc acccttgacg gccccaagcc
      181 ggtacacgac gccttcagac cctttcggtc cggtaagggc tcgtttgaca caattgtccg
      241 aaacatcaaa gaggtctgtt cccaaatcga cgttcaactg ggcggaaatt acacccgaga
      301 ccattaccgt caattccccg gtcttctcga ttatcttctc gattccggtc tcactcctgc
      361 tgatatttca aacgtcaggt tcgatccagt agtaaacgaa agccgtgaaa tggctccccc
      421 cgattttcac gacggctgca tgtccttcga cgaaccctgg cttcgggatg ccgccgtctt
      481 tctgagagcg gagatactga agcggggctt tcggacacag aaggtcgaac cgtccgtctg
      541 cttcgtggag cgcaggaaca acgtcgtggt caactacgac ggtgccttgt accggtgccc
      601 ggggcttatc ggccgaagag attattgcac gggctctgta aaaaccggca gcgctgacta
      661 tcgcgggacc cacaatctcg atagctggaa gaacgaagag tgcctctcat gctcctatct
      721 cccgctctgc ttcggcgggt gcaggtatat gaaactggtg cgggacggaa acatgcaggg
      781 cgttgactgc aagaaggcgt atttcgaccg gacacttgaa gctcttgtca tgcaggacgt
      841 gaaatacgac aagagtgacg aattgtgata cactcgaatc tatctattgc actgccgctc
      901 taacgcgagc tacatccatc aatgtccatc ctgccccctc tcatgagcaa cggaaagctc
      961 aaagccaccc ttctcgcggc cacggtcatg gcttgccttg ttctccttcc ctgccccgct
     1021 tccgcaaaag acgtcatcat cattaaagaa gcggaaatca aaccgttccg cgacgcaata
     1081 gaaggattca aaagctcctg cggctgccgc gccgaggaag tggcgttttc cgataagaac
     1141 ctgctcaaga gaatcgagaa agcccgtccc agcgccgtct ttgttgtcgg gacccaggcc
     1201 ttcgagaaag cgacggccat cacggacgtt ccggtcatct acaccatggt tatgccgtcg
     1261 gaggaggcct tcctccccgc ggacaacgtg tccggcgtcc gcatggacgt agcgcccgaa
     1321 acctggctta aaaccatgac cgcgctcttc ccgggtgtca aacgcatcgg cgtcctctcc
     1381 gatccggagc tcaccggccc gctcgtcagg gatgcggcgg ctgcggcgcg ggcactgggg
     1441 attacgctcg tcgtcaaatc aatcagcgat ccccgtcatg ctcctgagct cctgaatgag
     1501 ttccagaacg agatcgacct tttctggatg ctgcccgatc ccaccgtcgt cacgtccgaa
     1561 actttggatt acctgatgct tttttcgttc caaaacaacc ttccggtgtt ctcgttctcg
     1621 aaaaaatacg tcaaaaacgg cgccgccgcc gccctcgcca tcgatccctt tgacatgggc
     1681 gtgcaggcgg gagaacagta ccggacactg tccaagggcg gcaaaggccc gctccgcgcc
     1741 tatgcaagga cctcccggct gatcgtgaac cgaaaggtaa tcacaaagat gaaggtgcag
     1801 acaagcggtg aaatgctgca aaacgcggaa tctgtggagt gacgaatgga tctctttccc
     1861 cgtaaaagcc tgacattcag gctgctcagc tcatttgtct tcatcatcgt gattgtgctc
     1921 acgatccaga cggtcgtgta cattctccgg gcgcagaaaa ggatccacgg agaactggtg
     1981 cggcaggggg aactgctcat cgacctcctc gcctacagtt cccgcatcgc cgtcttcacg
     2041 gaaaacacgg agaagctcca ggaagacgcg gccggcatgc agacggagca ggatgtcgtc
     2101 ctggtcggat tctacggcag caagaccagc ccgctctacg tcacgagcct cacggaccgc
     2161 tatcgtctct cttccgatat cgacatcagc gccgccatca aacccggcga aaccttgatg
     2221 cgggaagaga cagataccgt ggaattcatc aagccggtcg ccctcctggt gaatccggac
     2281 gcaacttccc tg
//
LOCUS       DASSLD010000058         3384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1624011, whole genome shotgun sequence.
ACCESSION   DASSLD010000058 DASSLD010000000
VERSION     DASSLD010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624011"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VFK23_01070"
     CDS             <1..1195
                     /locus_tag="VFK23_01070"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7317704.1"
                     /translation="SGGELLAEIKAGQPDFPVIVITAFGTIENAVDAMKKGAFNYLTK
                     PVNPDELVSVAREALEKYELKRENAALKSELRQKYTFSSIIGKSAGMQEVFDMVRTVA
                     KTHSSVLIVGESGTGKELVARAIHYDSDRAGGSFVTVDCAAIPREIMESELFGHEKGA
                     FTGAHERKTGLLEHADGGTVFLDEIGDLDLNLQKKLLRFLQEREILRVGGTGRIKLNV
                     RIIAATNKELEQEVTAKNFREDLYYRLNVVTIWMPPLRERKDDIPLLAQHFLDKVSSV
                     EGKLVKGFEETVLDALLAYDWPGNVRELENVVERAYILCPNITITRNYLPSKLQGPDG
                     RGRGSFDEMNLLETEKRLILKALGTTSWNQSKASEILGITRKQLRTKMKNLGILPNSE
                     TAKKN"
     gene            complement(1307..1678)
                     /gene="rnpB"
                     /locus_tag="VFK23_01075"
     ncRNA           complement(1307..1678)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFK23_01075"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1768..2172)
                     /locus_tag="VFK23_01080"
     CDS             complement(1768..2172)
                     /locus_tag="VFK23_01080"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HET7317705.1"
                     /translation="MELTLFTDGASRNNPGEAGAGIFILRDGKPVGKIARYLGTTTNN
                     IAEYQAAIIGLEHCVKLGASTVRLHADSELMVKQLNGQYKVKNEGLKPLYEKAKELIA
                     KIGKVEVKYIPREQNKEADALANKAIDEKIAN"
     gene            complement(2260..2985)
                     /locus_tag="VFK23_01085"
     CDS             complement(2260..2985)
                     /locus_tag="VFK23_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HET7317706.1"
                     /translation="MDEQLGMLIQLQEMDGKIRSLADQNKRLPEILAGLESRRAANKA
                     ELETVKENLLAAQKSKRDRDQDLEAGIQKVEKLKARTSEIKNNKEYQALLKEIETVEQ
                     ENKAVEDDILALMEKIDAAAAQITAAEQKAKEEEVSIQAELKQHEATFAKLEVELKGV
                     EQARQELAARIDPSVLSSYQKLLLTKGGVAIAEARGENCSGCYMSIPPQVFVNVKKNE
                     SIITCPNCHRILYHKETIVQKNQ"
     gene            complement(3093..3168)
                     /locus_tag="VFK23_01090"
     tRNA            complement(3093..3168)
                     /locus_tag="VFK23_01090"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3133..3135),aa:Ile,seq:cat)
BASE COUNT          678 a          993 c          927 g          786 t
ORIGIN      
        1 gtccggaggg gaactcctcg cggagatcaa ggcgggccag cccgatttcc ccgtgatcgt
       61 gatcacggcc ttcgggacca tcgagaacgc cgtggacgcg atgaagaagg gggccttcaa
      121 ctatctcacg aaaccggtga accccgacga actggtgagt gttgcccggg aagcgctgga
      181 gaagtacgaa ctcaagcgcg agaatgcggc cctcaaatcc gaactcaggc agaagtacac
      241 cttcagcagc atcatcggca agagcgcggg catgcaggag gtcttcgaca tggtccggac
      301 ggtggcgaag acgcactcga gcgtgctcat cgtcggcgag agcggcacgg gaaaagagct
      361 ggtggcacgg gcgatccact atgactcgga ccgggcaggc gggtccttcg tcaccgttga
      421 ctgcgcggct atcccccggg aaatcatgga gagcgaactc ttcggccacg aaaagggggc
      481 cttcaccggg gcccacgagc gcaagaccgg tctcctggag catgccgacg gcggcacggt
      541 gttccttgac gagatcggcg acctggacct caatctgcag aagaagctcc ttcgcttcct
      601 ccaggagcgg gagatcctgc gggtcggcgg aaccggcagg atcaaactca acgtgcggat
      661 catcgcggcg acgaacaagg aactggagca ggaggtcact gcgaaaaact tccgcgaaga
      721 cctgtactac cggctgaacg tggtcaccat ctggatgccg ccgctccggg agcgtaagga
      781 cgacatcccg cttctggcgc agcacttcct cgataaagtg agcagcgtcg aaggaaagct
      841 cgtcaagggc ttcgaggaaa cggtcctgga cgcccttctt gcctacgact ggcccggcaa
      901 cgtacgcgaa ctcgagaacg tcgtcgagcg ggcctacatc ctctgcccga acatcaccat
      961 caccaggaac tatcttccgt caaagctcca gggtcccgac ggacggggac gtgggagctt
     1021 cgacgagatg aacctgctcg agacggagaa gcgtctcatc ctcaaggccc tgggcacaac
     1081 gtcctggaac cagagcaaag cctccgagat cctggggatc acacggaagc agctccggac
     1141 aaagatgaaa aacctgggga tcctgcccaa cagcgaaacc gcaaaaaaaa attgaatgtc
     1201 ctctattcct cggaatgcgg gttgcggaat ggaccacgcc ggagccttct ccagcatggt
     1261 gaagccgggg ggagtggcat aaaaggattg ctgcggcatg aaaaaaaatc agagcgggcc
     1321 gtaagccgag ttctgtcccc cgatcactcg gggtggtgat catttatcta ggcccccggt
     1381 tacccgaggg ctcatgcgac ctacccggaa gtctcggacg ggccgtcctt acccccttgc
     1441 gggggacgct tccctatgtg gtcttgctcc aggcggggtt ttccaagcac tcccggtcgc
     1501 ccgggagtct ggtgggctct tgccccaccg tttcaccctt accccgttac cggggcggtc
     1561 ttcttttctg tggcactgtc catggggtcg ccccctgtcc cgtttcggga ccgccttgcc
     1621 ctgcggagct cggactttcc tccccccgtt tccggagggc gatcacctca cccgctctga
     1681 atcaaagaac aacgataaat ttaaaattgg aaatttgaga ttgcataaat acccgtttca
     1741 gagtttacaa tttgaaattc ccaatcttca atttgcaatt ttttcatcaa ttgccttgtt
     1801 cgccagcgca tccgcttctt tgttttgctc ccgtggtatg tattttacct cgaccttacc
     1861 gatctttgca atcaattcct tggccttttc gtacagcggt ttgaggcctt cgttcttcac
     1921 cttgtattga ccgtttaact gtttcaccat aagctcggaa tcggcgtgta atctgaccgt
     1981 tgaagcgccg agtttcacgc aatgctccag tccgataatg gccgcttgat attcagcaat
     2041 gttattcgtt gttgttccga ggtaacgcgc gattttgccc acgggtttgc catcccggag
     2101 gataaaaata cctgcgccgg cctctccggg attattccgc gatgcgccgt cggtaaagag
     2161 ggttagttcc atatttgagt tatcgtttct ccatgaaaaa catcctgtcg cagataacgg
     2221 catatgtaca ggcctatctg cttgcaggct tcttcgcgct tattggtttt tctgcacgat
     2281 cgtttccttg tggtagagta tccggtggca gtttgggcag gtgatgatgc tttcgttttt
     2341 cttgacgttg acgaaaacct ggggcggtat gctcatatag cagccggaac agttctcgcc
     2401 gcgtgcctcg gcaatggcca cgccgccttt cgtcagaagc agcttctggt acgaggaaag
     2461 gaccgaggga tcgatgcgtg cagccaactc ctgacgggcc tgttcaacgc ccttcaattc
     2521 cacttcgagc ttcgcgaacg tcgcctcatg ctgcttcagt tccgcctgga tcgagacttc
     2581 ctcctccttc gccttttgtt cggcagcggt tatctgcgct gccgctgcgt cgattttttc
     2641 catcagcgcc aggatgtcgt cctcgacggc cttgttctcc tgctccaccg tctcgatctc
     2701 cttgagcagc gcctgatatt ctttattgtt cttgatctcc gaagtacggg ccttcagttt
     2761 ttcgactttc tggatgcccg cctccaggtc ctggtcgcgg tcgcgcttgc ttttctgagc
     2821 cgcaagcagg ttctctttta cggtttcaag ctccgccttg ttcgcagcac gcctgctttc
     2881 gagccccgcc aggatctcag gcaggcgctt gttctgatcc gcgaggctgc ggattttccc
     2941 gtccatttcc tgaagctgaa taagcatgcc gagctgttcg tccattatac ctccaaaatg
     3001 aaaatgcgcc tctcggcgca tttctgcagg acgcttgctg cctcatggga ttgttgttcc
     3061 atacactgca ttcccttccc cgccggtgaa gttggtgggc ccaccaggac tcgaacctgg
     3121 gaccaaccgg ttatgagccg gtagctctac cagctgagct atgggccctg ctgagctgca
     3181 aattccggat tgccggcact gggccacgag gcgcaaaacg cgtaaaaatt ttgtgcctcg
     3241 ttcctggagc cgtttggcgg tgtcatgctg gtgaacccgt aggtgcccgc ctcgaaactt
     3301 ggagcaccat attgtcacaa cctccggcaa atgtcaagac tttgccttcg acaaccctac
     3361 tccatgaagc tcttgagctg cttg
//
LOCUS       DASSLD010000059         3779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1395945, whole genome shotgun sequence.
ACCESSION   DASSLD010000059 DASSLD010000000
VERSION     DASSLD010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395945"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFK23_01095"
     CDS             <1..180
                     /locus_tag="VFK23_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HET7317707.1"
                     /translation="IHELRASLYDLDDRPLVTSFMGGLGGETIWPADFDYMAGVLTQM
                     VKDKRTDRYVYWLGM"
     gene            217..1203
                     /locus_tag="VFK23_01100"
     CDS             217..1203
                     /locus_tag="VFK23_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7317708.1"
                     /translation="MPAFDYKFQQLEPIRSFKKVPEAEQYLPGHRTCAGCGPALNYRL
                     VAKAAGKDTIFVGPTGCMYVANASYLCTPYAVSWIHAQITNAGAVASGIEAAFKMAAR
                     KGRHTGPFPNIIVMGGDGGSVDIGLQAMSGMMYRGHKVLFICYDNESYANTGIQTSPT
                     TPYGAATTFTPPGKEIPEGKTLFPRNVPQMVIGGHPAVKYVATTTIAHPLDLMNKVRK
                     ALSKPGPTFLHILCPCPKGWLFDEKLTPDMAKLAVETGMWSNYEWEDGVYKYTQVPKK
                     YKPVSDYMKPQGRFAHLKPEHIAKMQAFVDEKIKQVQRPIPVAAAVPAKQSA"
     gene            1310..2299
                     /locus_tag="VFK23_01105"
     CDS             1310..2299
                     /locus_tag="VFK23_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET7317709.1"
                     /translation="MNKAISRAGTGTVLVCSLVLMCLSAGTAVAWQPDKPVELIVPAG
                     SGGGADVMARFVTPIIDKYKLSPKPFIVVNKSGGAGAEGFMYVKGKKGDPNVIIITLD
                     NLFTTPLATGVPFTWKDLTPVSRLALDYFVLWVNVESPYKTAQEYLDAVKKEPGKFNM
                     GGTGSKQEDQIITVQFEQLFGDKFSYVPFKGGGEVAVELVGKHVDSTVNNPAEAVSHW
                     KAGRLRPLATIDHERINLPEWKTIPTMKEATGKDMSYLMLRGFFAAPGITKEQQDYYA
                     GVFKKVSETPEWKKYTSDMGLKDAYLAGPEYVKWLEEKDKTTQDLMRKGGLIK"
     gene            2419..2997
                     /locus_tag="VFK23_01110"
     CDS             2419..2997
                     /locus_tag="VFK23_01110"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HET7317710.1"
                     /translation="MRKAQIIVAFFFVAIGVIVIVDAIRLGYGWGPSGPESGFFPFYL
                     GVGTLICSALVLIKAFKEPTAANLEATLERIRRVVATTAGGVPEERVRLLEAEARRIA
                     GKRLIPEGGLKPILWVLLPSAAMVLLTELVGIHIAAAIFLAFYMRAVGKIRWITVALV
                     SILLPVSLYVVFDKMFLIPLPTGLWGSKLIPF"
     gene            3016..>3779
                     /locus_tag="VFK23_01115"
     CDS             3016..>3779
                     /locus_tag="VFK23_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015420716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HET7317711.1"
                     /translation="MVENLHHLLAGISTAIQPGNFLIAMVGLVLGVIVGVLPGLGGAN
                     GVAILIPLTVTMKPVPGIILLSSIYWGALYGGSITSILFNIPGEPWSVATTFDGYPMA
                     KKGKAGKALVLAFLSHGVGGILGIIMLSFFAPIIAEFALKFGPAEIFSVMMLTFSAFV
                     GLGGKSPTKTLIAIFFGFILAVVGMDIDTGQLRLTFGSVELMGGFNFIVAVIGLFGLG
                     EIFLTVEEGLKMEGIAAKVRLQDIVEGLKDLATYWKT"
BASE COUNT          750 a         1130 c         1204 g          695 t
ORIGIN      
        1 attcacgagc tccgggccag cctctatgac ctggatgacc gtccactggt cacatcgttc
       61 atgggcggcc tcggcggcga gaccatctgg cctgccgact tcgactacat ggcgggagtc
      121 ctcacccaga tggtcaagga caaacggacc gaccgatacg tctattggct cgggatgtag
      181 gccgaatggc attcaggaaa gcattacgga ggaaacatgc cagcatttga ctacaagttt
      241 cagcagttgg aacccattcg gtccttcaag aaggtgcccg aggcggagca gtatcttccc
      301 ggccatcgga cctgcgccgg ctgcggcccg gccttgaatt accggctggt cgcgaaagcg
      361 gcgggcaagg acacgatctt cgtcggcccc acgggctgca tgtacgtcgc gaacgccagc
      421 tatctctgca ccccatatgc cgtgtcctgg atccacgccc agatcacgaa cgccggcgcc
      481 gttgcgtcgg ggatcgaggc ggcattcaag atggcggcgc gcaagggccg gcacacaggg
      541 ccgttcccga acatcatcgt gatgggtggt gacggaggca gcgtcgacat cggcctgcag
      601 gcgatgtccg ggatgatgta ccgcggtcac aaggttctct tcatctgtta cgacaacgag
      661 tcctacgcga acaccgggat ccagacgtct cccaccacgc cctacggtgc cgcgaccacc
      721 ttcacccctc cgggcaagga gattcccgag ggaaagaccc ttttccccag gaacgttccg
      781 cagatggtta tcggcggtca cccggcggtc aagtacgtcg ccacgaccac gatcgcgcat
      841 ccgctcgacc tgatgaacaa ggtccggaag gcgctgagca aaccggggcc gacgtttctg
      901 catatcctct gcccgtgccc caagggctgg ctgttcgatg agaagctgac gcctgacatg
      961 gccaagctgg ccgtggaaac gggaatgtgg tcgaactacg aatgggaaga tggcgtgtat
     1021 aagtacacgc aggtaccgaa gaagtacaaa ccggtcagcg actacatgaa gccccagggc
     1081 cgcttcgccc atctcaagcc ggagcacatc gcgaaaatgc aggcgttcgt ggacgagaag
     1141 atcaagcagg ttcagcggcc gatcccggtg gccgccgccg ttcctgccaa acagtcggcc
     1201 tgagccggca agaaatcgag gatcacgacg accagaggac agaggacggg taagaacagg
     1261 cgattcgcag caacaccgaa acttcttttc acatagggag gtcggaaaca tgaataaggc
     1321 gatttcgcgg gcggggaccg gcacggtcct cgtttgctcc ctggttctga tgtgtctttc
     1381 ggccggcacg gcggtcgcat ggcagccgga caagcccgtg gagctgatcg ttccggcggg
     1441 ctccggcggc ggcgcggacg tcatggcccg attcgtcacg cccatcatcg acaaatacaa
     1501 gctgtccccg aagcccttca tcgtcgtgaa caagtccggc ggcgccggag cggaaggatt
     1561 catgtacgtg aaggggaaga agggtgatcc gaacgtcatc atcatcaccc tggacaacct
     1621 gttcacgact ccgctggcga cgggcgttcc cttcacgtgg aaggacctca ccccggtttc
     1681 gcggctcgcc ctcgactatt tcgtcctctg ggtgaacgtc gaatctcctt acaagaccgc
     1741 ccaggagtac ctggatgcgg tcaagaagga gcccggaaaa ttcaacatgg gcggcaccgg
     1801 ctcgaagcag gaggaccaga tcatcacggt ccagttcgag cagctgttcg gcgacaagtt
     1861 ctcgtacgtg ccgttcaagg gcggcggcga ggtggccgtc gagctcgtcg gcaagcacgt
     1921 cgactccacg gtgaacaatc ccgccgaagc cgtcagccac tggaaggcgg ggcggctgcg
     1981 cccgctggcg accatcgacc acgagcggat caatctgccg gaatggaaga cgatcccgac
     2041 gatgaaagaa gcgaccggca aggacatgag ctacctcatg ctgcgcggct tcttcgcggc
     2101 gcccggcatc accaaggaac agcaggatta ctacgccggg gtgttcaaga aggtctccga
     2161 gactcccgag tggaagaagt acacgtccga catggggctc aaggatgcct acctcgccgg
     2221 cccggagtac gtcaagtggc tggaggagaa ggacaagacg acgcaggacc tcatgagaaa
     2281 aggcgggctc atcaagtagc gacgctttct gttcacgaag gatcgggaag caaacgtcgc
     2341 atccggaggg aggcgggcgg ttttcgcctg cctcctcgtc cggctttggc ttcctggcgg
     2401 ctcggagagt gagggaagat gcggaaggcc caaatcatcg tcgccttttt cttcgtcgcg
     2461 atcggggtga tcgtcatcgt cgacgccatc cggctcggct acggatgggg accgagcggt
     2521 ccggagtccg gcttctttcc gttttatctg ggcgtgggca cgttgatctg cagtgcgttg
     2581 gtcctgatca aggcattcaa ggagcccacg gccgcgaacc tcgaagccac cctggagagg
     2641 atccggcgcg tcgtcgccac gacggcgggt ggcgtccccg aggagcgggt gcggctcctg
     2701 gaggccgagg ccaggcggat cgccggaaag cgtctgatcc cggaaggagg gctcaagccg
     2761 atcctctggg ttctgctccc ctcggcggcg atggtcctcc tcacggagct cgtcggcatt
     2821 cacatcgcag ccgcgatatt cctcgcgttc tacatgcggg cggtgggaaa gatccggtgg
     2881 atcaccgtgg ccctcgtgag catcctgctt cccgtttctc tctatgtcgt cttcgacaag
     2941 atgtttctga ttccgctgcc gacggggttg tggggcagca aactgatccc attctgaaat
     3001 cggcaggaga aaagcgtggt cgagaatctc catcacctcc tcgcgggcat cagcacggcg
     3061 atccagccgg gcaatttcct gatcgcgatg gtcgggctgg ttctcggggt gatcgtcggc
     3121 gtcctgcccg gcctcggtgg agcgaacggg gtggccatcc tcatcccact gacggtgacg
     3181 atgaagcccg tgccgggcat catcctgttg tcgagcatct actggggagc gctctacggc
     3241 gggagcatca cgtcgatcct cttcaacata cccggagagc cgtggtcggt cgcgacgacc
     3301 ttcgacggct atcccatggc aaagaagggg aaggccggta aagcgctcgt gctggcgttc
     3361 ttgtcgcacg gggtgggcgg gatattgggc atcatcatgc tcagcttctt cgccccgatc
     3421 atcgccgagt tcgccctcaa gttcggcccg gcggagatct tctccgtgat gatgctcacg
     3481 ttcagcgcgt tcgtcggcct cggagggaaa tccccgacga agaccctgat tgccatcttc
     3541 ttcggattca tcctggccgt ggtcgggatg gacatcgaca ccggacagct ccggttgacg
     3601 ttcgggtccg tcgagctgat gggagggttc aacttcatcg tcgcggtcat cggcctgttc
     3661 ggcctggggg agatcttcct cacggtcgag gaaggtctca agatggaggg gatcgccgcg
     3721 aaggtgaggc tccaggacat cgtggagggg ttgaaggacc tcgccacgta ctggaagac
//
LOCUS       DASSLD010000060         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1069092, whole genome shotgun sequence.
ACCESSION   DASSLD010000060 DASSLD010000000
VERSION     DASSLD010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069092"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VFK23_01120"
     CDS             complement(<1..1087)
                     /locus_tag="VFK23_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7317712.1"
                     /translation="MKKRASPPAPAVLRRKAQKKLKEQPQRLSALSTRDMKHLITDLG
                     THQIELEMQNEELRSTQMELDASSRKYAELFDFAPVGYFTLDTRGMIQDVNRTGAELL
                     GLDKSKLRGKSFSGFLSDPEDQRVLREHCKEVFLKQKPQKCEVRLRREKAGPLYVQLQ
                     SMAAENVDGKAGLVRTAVMDITDRRLAEESLIESEHRFRTLFNGMTEGFALFETLSDQ
                     KGKLVDFRCVEVNPAFELLTGLKRSDVIGKTHRQVVPEDASFFKTYSDVARTGQPAHF
                     DRWSPALKRHYDVYVYSPASGQIALLFLDITKRKQAERALKEGELRLQLAVSAMNLAI
                     WEWDPVNNKTVKSDIYAAQYGRPPETAS"
     gene            complement(1307..1720)
                     /locus_tag="VFK23_01125"
     CDS             complement(1307..1720)
                     /locus_tag="VFK23_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7317713.1"
                     /translation="MTIREKMHRLPREFYQGPVSVAFTLCVKDRAKAFSVPHVVDAFN
                     DMLSDVATKTGCIVPVYCFMPDHQHVVITGTHSGSDTWKAVVSYKQKTGYWLSVNRPG
                     TEWQKDFYDHVIQSQKGFAARVKYILDNPVRKQPG"
     gene            complement(1796..>2216)
                     /locus_tag="VFK23_01130"
     CDS             complement(1796..>2216)
                     /locus_tag="VFK23_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7317714.1"
                     /translation="LVLTFVDITDQKRAEQLEANIVTYLHGIMDTVREPFMVLDRNLL
                     VISANRAFYDFFRTAPEETEKRPVYELGNGQWEIPALKKLLDGVLSSDRPFENYRVEH
                     DFPQAGRKIMLLNARRIYQEGLKTDTILLAMEDVTGK"
BASE COUNT          450 a          613 c          596 g          557 t
ORIGIN      
        1 gggacgccgt ttccgggggg cggccgtact gtgcggcata gatgtcgctt ttaaccgtct
       61 tgttgttgac ggggtcccac tcccagatgg ccaggttcat ggcgctgacg gccagctgca
      121 gccgaagctc accttctttg agcgcccgct ccgcctgctt acgcttggtg atgtccagga
      181 acaggagggc gatctgcccc gacgccggac tgtagacgta gacatcataa tgcctcttga
      241 gcgccggaga ccacctgtcg aaatgggcag gctggccggt ccgggcgaca tcgctatagg
      301 ttttgaaaaa agaggcgtcc tcgggcacga cctgccgatg ggtcttgccg attacatcgc
      361 tccttttcaa accggtcaac agttcgaatg ccgggttgac ctccacgcac cggaaatcga
      421 cgagcttgcc cttctgatcg gacagggttt caaacagggc aaacccctcg gtcatgccgt
      481 tgaagagcgt acggaagcgg tgctcgctct cgatgagcga ctcctccgcc agcctgcggt
      541 ctgtgatgtc catgacggcc gtcctgacca atccggcctt tccgtcgaca ttctcggctg
      601 ccatgctttg caactgaacg tacagagggc cggccttctc cctcctcaag cggacttcgc
      661 acttttgagg tttctgcttc agaaagactt ctttgcagtg ctcccgtaac acgcgctggt
      721 cttcgggatc ggacaggaag ccggagaatg attttccgcg caacttgctt ttgtcaaggc
      781 cgaggagttc cgcaccggtt ctgttgacgt cctgtatcat gccccgcgta tccagcgtga
      841 aataaccaac gggcgcgaaa tcgaacagtt cggcatattt ccggctggag gcgtcgagtt
      901 ccatctgggt gctgcgaagc tcttcgttct gcatctcaag ctcgatctgg tgcgtgccga
      961 gatcggtgat gaggtgcttc atgtccctcg tggacagtgc gctcaacctt tgcggctgct
     1021 ccttgagctt cttttgcgcc ttgcgccgca ggacggccgg tgcagggggg gatgcccttt
     1081 ttttcatgat ttttcccatt ataggcccaa ataaaaaaac ggatcgatgc attcgatccg
     1141 ctacttgaat aagtatacta cattttttta tgcagttgtt agtttgtagc cgcaggcttt
     1201 agcctgcgaa aaaaacagac gcaggccaca caatcacatc atgccgttta aaatgtcgtc
     1261 aagtctgcat cctatggaac ctgtatacgg gtagtcctcc cagttcttaa ccaggttgtt
     1321 ttctaactgg attgtcaagg atgtacttaa cgcgggcagc gaagcctttc tgagattgga
     1381 tgacatgatc ataaaaatcc ttctgccact cagttccagg tctattgact gagagccaat
     1441 aacccgtctt ctgcttgtaa ctcacaactg ccttccacgt gtcggatcca gaatgcgttc
     1501 ccgtgatcac aacatgctgg tggtccggca taaagcaata gacaggaaca atgcagcccg
     1561 tttttgttgc aacatccgac aacatgtcgt taaaggcgtc gacaacatga ggtacgctaa
     1621 acgccttcgc gcgatccttt acgcatagtg tgaaggcaac ggataccggt ccctgataaa
     1681 actcgcgggg cagtcgatgc attttttccc ggatggtcat atcgttgtct ttgtagcctc
     1741 atccttcatg gtgcgtcgtt ttcgttatgc gcaggctgaa gcctgcggct acgaatcatt
     1801 tccccgtaac atcctccatt gccaaaagga tcgtatcagt cttgagccct tcctggtaga
     1861 tgcgtctggc gttcaggagc atgatcttac ggccggcctg cgggaagtcg tgctcgaccc
     1921 ggtagttctc gaacggtctg tcgctcgaga gtacgccgtc cagaagcttt ttcaaggccg
     1981 ggatctccca ctggccgttc cccagttcgt agacaggccg cttctcggtt tcttcgggcg
     2041 cggtcctgaa aaaatcgtag aatgcgcggt ttgccgaaat cacgagaagg tttctgtcca
     2101 gcaccatgaa gggttcgcgc acggtgtcca tgatgccgtg caggtatgtg acgatattcg
     2161 cttcaagctg ttcggcccgc ttctggtccg tgatgtccac gaaggtaagg accagg
//
LOCUS       DASSLD010000061         4409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1297771, whole genome shotgun sequence.
ACCESSION   DASSLD010000061 DASSLD010000000
VERSION     DASSLD010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297771"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..929)
                     /locus_tag="VFK23_01135"
     CDS             complement(21..929)
                     /locus_tag="VFK23_01135"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HET7317715.1"
                     /translation="MEDHKLRVFCAVAETKSFSKASELIHLTQPAVSLQVQAMEELYE
                     TRLFDRSGNSINLTPAGEILYRRAKEILALYAEAQKNISEITGAIKGSLSIGASSTIG
                     NYLLPQIISAFKKKVPQVNISLVVGNTKTITEKLNAGEIDVALVEGDVSKQRFSVDTL
                     VSDELVVIMSPAHPWAERRNIPAMDLIKEPLILREEGSGTRQIILKHLDDHGIKLDHL
                     RIALVLGSTESIKSAVEEGMGISILSAWAARQAIKHGTLKAMTFKDVRFHRNFSIISP
                     KRNYCTHTAKEFLSFLKVYPIKSPMS"
     gene            1167..1340
                     /locus_tag="VFK23_01140"
     CDS             1167..1340
                     /locus_tag="VFK23_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317716.1"
                     /translation="MAEMSKTAKAATKKAVVNLNVPQCCGKPMQAAKMMTTTGKVRGM
                     MWLCDKCFKEKAI"
     gene            1643..2260
                     /locus_tag="VFK23_01145"
     CDS             1643..2260
                     /locus_tag="VFK23_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HET7317717.1"
                     /translation="MALFPKKIDFFEQFDRALENLSRTANVLVDTFNNFENFEKKAKV
                     IYEFEQEGDMLTHDIMKDLNKTFLTPIDREDIHALASRIDDVVDLIWATVDRMVVYKI
                     EKPTPEVISIAEDLQMTADVLKKALRELRAKQYSHVQEHCIEVNRLENRIDRKYRDAL
                     GRLVNDRNDPVYIIKWKDIYQLFEDASDRAEDVANILEGIVLKNA"
     gene            2253..3242
                     /locus_tag="VFK23_01150"
     CDS             2253..3242
                     /locus_tag="VFK23_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HET7317718.1"
                     /translation="MHEILILIILLAVFFDIANGWHDCANAIATVVSTKVLSPMMAVV
                     LAAGMNILGAFLSTAVAKTIGSGIVDPKAVTDIVIISALISAILWNIMTILMGLPVSS
                     SHALFGGIAGAAMAHGGVSILNGGGIVKILTSLLISPVFGIVIGYLFMKLILYLFGHL
                     TPGIISKYFGRLQILSSAFMAFGHGSNDAQKAMGIITMALVSGGAIKTLDVPTWVILI
                     CALAMGFGTLFGGWKIIKTLGVKMLKLEPIHGFAAETSSAMIIVAASHWGMPVSTTHV
                     VSSAILGVGATKRLSAVRWGIAGKIVMAWVLTLPACIAMAWGIQKALTYYRLG"
     gene            3287..3697
                     /locus_tag="VFK23_01155"
     CDS             3287..3697
                     /locus_tag="VFK23_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317719.1"
                     /translation="MVDNDSIKAFVQNTLGCGCPDEVFRLVDWRPHVHLSDEVTLAFA
                     VTIGDRLLVYVTERDAVLDEVLLTFLISRGKEERDARGLNRFRLVVATDDAAIREGLM
                     RKFEELCDKDEKIHLHLITKADNIFTAGVGQHSG"
     gene            complement(3694..>4409)
                     /locus_tag="VFK23_01160"
     CDS             complement(3694..>4409)
                     /locus_tag="VFK23_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET7317720.1"
                     /translation="PCLILLFFESRHFDVAIAAAGVVYLLPMICKIDLHVHSKYSGDN
                     DADPEETIIRAIESGLQGIAFTEHYYYEASEQAEILKEKYGGRILILRGVEFSADEGH
                     CLVFGADTDKLGIKYAPVADVIRFVGEAGGVVIPSHPYRPGTSLGNLVQSMNGITGLE
                     GCNGANMYTFNVKAIQAAKTLHLPYTGGSDAHEPREVGSCYTEFRHAVSYENFIDSLK
                     AGDYLGVDTRKISRMFNLP"
BASE COUNT          949 a         1271 c         1236 g          953 t
ORIGIN      
        1 gaatatgcgc aggctctcga tcaggacatg ggagatttga tggggtatac cttgaggaag
       61 ctcaggaact ccttggccgt gtgggtgcag tagttgcgct tcggcgagat gatgctgaag
      121 ttccggtgga acctgacgtc cttgaacgtc atggctttga gggtgccgtg cttgatcgcc
      181 tggcgggccg cccaggcgga caggatggag attcccatcc cctcctcaac cgcgctcttg
      241 attgattcgg tgctgccgag aacgagagcg atccggaggt ggtccagctt gatcccgtgg
      301 tcatcgagat gtttcagaat gatctggcgc gtgcccgacc cctcttcccg caggatgagc
      361 ggctccttga tcaggtccat ggcgggtatg ttccgccgct cggcccaggg atgggcggga
      421 gacatgatca cgaccagttc atcggacacg agcgtatcca ccgagaaccg ctgcttgctc
      481 acgtcccctt caaccagggc cacgtcgatc tcgccggcgt tcagcttttc ggtgatcgtc
      541 ttcgtgttcc cgaccacgag cgagatgttg acctgcggga cctttttctt gaacgcggag
      601 atgatctggg ggagaaggta gttgccgatc gtggaactgg cgccgatgga gagggaccct
      661 ttgattgcgc ccgtaatttc gctgatgttc ttttgcgctt cggcgtaaag ggccaggatc
      721 tccttggccc gcctgtacag gatctcaccg gccggcgtga ggttgatgga gtttcccgag
      781 cggtcgaaaa ggcgcgtctc gtaaagttcc tccatggcct gcacctggag gcttacggcg
      841 ggctgggtca ggtggatcag ctccgaggcc ttggaaaagc tcttcgtctc cgcgactgcg
      901 cagaacaccc tgagtttgtg gtcttccatc gtttagtcag actctgctac ggacaggtta
      961 aaattcgctt gaactttcaa gcagttcgaa gtcgtcggcg tgtatggtat ttataaatca
     1021 aacacctccc gttgtcaagc agataatcga tgaaccgcct gtcccaacgc gccatagacc
     1081 acggaagggc aaaaacttct tgacattttc tcccgtatgg ttacaatgca cgggttttcg
     1141 ctaaacgaca cggaggtgat gcaatcatgg cagaaatgag taagaccgca aaagcggcaa
     1201 ccaagaaggc cgttgttaat ctcaatgtgc cccagtgctg cggaaagccg atgcaggctg
     1261 cgaagatgat gacgacgacg ggcaaagtcc gcggcatgat gtggctgtgc gacaagtgct
     1321 tcaaagaaaa agccatctaa gcccctaccg gatccggttt gtaccagggc ggtgaattca
     1381 ttcaccgccc tttttttttc ggccgggcca gccccggcct cctgccgtga gatgaagcag
     1441 cccccgtatc ccgcttaaat ccccataaag ttcacaaatg aaatcataaa ataatccgtc
     1501 ggggaagaaa cgacttccct gttgcaaata cgggctgctt gtgataactt atgggcgctc
     1561 gaaaaccgga cttccgaggc atgctgcgcg ggtccatgcc gccgtttcat ttcccgtccc
     1621 gaaggagtgt gcgaacagac ctatggccct cttcccgaaa aaaatagact tttttgagca
     1681 gttcgaccgg gcgctcgaaa acctgtcgcg cacggccaat gtcctggtgg acaccttcaa
     1741 taacttcgag aacttcgaga aaaaggcgaa ggtgatttat gagttcgagc aggaaggcga
     1801 catgctgaca cacgacatca tgaaggacct gaacaagacc ttcctcacac ccatcgaccg
     1861 cgaagacatc cacgccctgg cgtcacgcat cgatgacgtg gtcgacctga tctgggccac
     1921 cgtggacagg atggtcgtgt acaagatcga aaagccgact ccggaggtca tatccatcgc
     1981 cgaagacctc cagatgaccg cagacgtttt gaaaaaggcg ctccgggaac tgcgggcgaa
     2041 gcaatattcc cacgttcagg aacactgcat cgaagtaaac cgcctcgaaa accgcatcga
     2101 ccgcaagtac cgggacgccc tcggcaggct cgtgaacgac cggaacgatc cggtgtacat
     2161 catcaagtgg aaagacatct accagctctt tgaagacgcc tcagaccggg ccgaggacgt
     2221 cgccaacatc ctcgaaggca tcgtcctgaa aaatgcatga aatcctgatt ctcatcatcc
     2281 ttctcgcggt gtttttcgac attgcgaacg gctggcatga ctgcgcgaac gccatcgcca
     2341 ccgtggtatc gaccaaggtg ctgtcgccca tgatggcggt ggtcctcgcc gcgggcatga
     2401 atatcctggg cgccttcctc tcgacggctg tggcgaaaac gatcggctcg ggtatcgtcg
     2461 accccaaggc ggtcaccgac atcgtcatca tctccgccct gatctccgcc atcctctgga
     2521 acatcatgac gatccttatg gggcttcctg tcagttcctc gcacgcgctt ttcgggggca
     2581 tcgcgggagc ggccatggcc cacggcggcg tcagcatcct gaacggcgga ggcatcgtta
     2641 aaatactcac ctcgctcctc atctccccgg tcttcggcat cgtcatcggg tatctcttca
     2701 tgaagctgat tctgtatctt ttcggccatc tcacccccgg catcatcagc aagtacttcg
     2761 gcaggctcca gatcctctcg tcggccttca tggccttcgg tcacggctca aacgacgcgc
     2821 agaaggccat gggcatcatc acgatggccc tggtctctgg cggcgcgatc aaaacgctcg
     2881 atgttcccac gtgggtcatc ctcatctgcg ctttggccat gggtttcggc acgctcttcg
     2941 gcgggtggaa gatcatcaag accctcggcg tcaagatgct gaagctcgag cccattcacg
     3001 ggttcgcggc agagacgtca tccgccatga tcatcgtagc ggcaagccat tggggcatgc
     3061 cggtcagcac gacccatgtc gtctcatccg cgattctcgg cgtcggcgcg acgaagcgcc
     3121 tctcggccgt ccgctggggc atcgcgggca agatcgtcat ggcctgggtg ctgacgctgc
     3181 cggcctgcat cgccatggcc tgggggatcc agaaggccct gacgtattac cgtctcggat
     3241 agcggtcctc cgcagcgatg tccttcacga ggtattcctt ccgtccatgg tcgacaacga
     3301 ctccataaag gcatttgtcc agaacaccct cggctgcgga tgcccggatg aagtattccg
     3361 cctggtcgac tggagacctc acgtgcacct gagcgatgag gtgaccctgg cctttgcagt
     3421 cacgatcggc gaccgcctgc tcgtctacgt gaccgagaga gatgccgttc ttgacgaggt
     3481 gcttcttacc tttctcatat ccaggggaaa ggaggagcgc gatgcaaggg gattaaacag
     3541 gttcagactg gtcgtagcca cggatgatgc ggcgatccgg gaaggattga tgcgtaagtt
     3601 tgaagagctc tgcgacaagg acgaaaagat ccatctgcac ctgatcacga aagcagacaa
     3661 catcttcact gcaggggttg ggcagcattc cggctagggt aggttaaaca tcctcgatat
     3721 cttcctcgta tccacaccca gataatctcc cgccttcagc gagtcgataa aattttcgta
     3781 cgacacggcg tgacggaact cggtatagca ggaacccacc tcgcggggtt catgcgcgtc
     3841 cgacccgccg gtatagggaa gatgcaacgt ctttgccgct tggatcgcct tgacattaaa
     3901 ggtgtacata tttgcgccgt tgcacccttc aaggccggta atgccgttca tgctctgcac
     3961 caggttgcca aggctcgtcc ccggccgata gggatgggag gggattacca cgcctccggc
     4021 ctcgccgacg aagcgtatca cgtccgcgac gggagcatac ttgatgccca gcttgtccgt
     4081 gtcggctccg aaaaccaggc agtggccttc gtctgccgag aactcgacgc cccgaagaat
     4141 caggatccgt ccgccgtact tctccttcag gatctcggcc tgctccgacg cttcataata
     4201 ataatgctcg gtgaaagcga ttccctgaag gccggactcg atggctcgga ttattgtttc
     4261 ctctggatca gcatcgttgt cgccgctgta cttcgaatga acgtgaaggt ctatcttgca
     4321 gatcatgggt aacaggtata ccacgccggc ggctgcaata gcaacatcaa aatgccttga
     4381 ttcaaaaaag agcaaaatga gacacgggg
//
LOCUS       DASSLD010000062         3097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1623732, whole genome shotgun sequence.
ACCESSION   DASSLD010000062 DASSLD010000000
VERSION     DASSLD010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623732"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            634..1164
                     /gene="tpx"
                     /locus_tag="VFK23_01165"
     CDS             634..1164
                     /gene="tpx"
                     /locus_tag="VFK23_01165"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET7317721.1"
                     /translation="MERSGVITMKGKPLTVIGPDVRAGDRAPAFSVLDENLNSVSLAD
                     FAGKTKLISVTPSLDTPVCDLQLRRFNREAASMPRDVVVLNISMDLPFAVSRFCSTAG
                     IDRAKALSDHRDASFGAAYGVLIKELRLLARSIFIIDKNDVVRYKEIVPEQSNHPDYE
                     KALRALAEITSGVKAA"
     gene            complement(1256..1456)
                     /locus_tag="VFK23_01170"
     CDS             complement(1256..1456)
                     /locus_tag="VFK23_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET7317722.1"
                     /translation="MAKGTVKWFNDSKGFGFITKEDGGDVFVHFSDIQDSGFKSLSEG
                     QTVTFDVVDSPKGPKAANVVKQ"
     gene            1938..>3097
                     /locus_tag="VFK23_01175"
     CDS             1938..>3097
                     /locus_tag="VFK23_01175"
                     /inference="COORDINATES: protein motif:HMM:NF020028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317723.1"
                     /translation="MKLGRTVWDLSPLFAGDDDPRMEQKRKIVSRKGREFINAWKDRT
                     DYLEDPAVLLRALDQYESWKRLYGSDGDEGYYFWLRTQQDQLDPKLKARFNKIEEFSK
                     KIENDIQFFYLRIAKIKPEAQRRFLEDEGLAKYRHYLERIFAEARYHLSEPEEKILNL
                     KSSTSYSNWTRMTSGFLSREERAVTGEDGVKKIKPFSELTGLMNSRVKHVRDSAARAF
                     NDILQKHVEVAEAELNSVLQNKKIDDELRGAPRPDTLRHLADDIESDVVDALIDSVSR
                     RFDIPSRYYELKAALLRVKRLQYHERNVEYGVVAKRYPYPQAINLVWRVMNKLDGRFG
                     DILTGFVKNGQIDAYPRKGKDSGAFCMHHLLVQPTYILLNHTGKLHDVLMIA"
BASE COUNT          780 a          799 c          848 g          670 t
ORIGIN      
        1 gttcttacag caagagacat gccaagcctc tattaaaaac ttatttcgtt attacacata
       61 gttacaaatg gagagggaaa aaggatagta gtttgaattg ctcattttga ttctgctggt
      121 ctattttgac cggtcaataa ctgaggcggt tgatttctat ttcgttgacg agctttttgt
      181 gagtaataaa gaagtgttac gggttgtgag ctccaacccc atacccgtag tccatcttcc
      241 caggaaaccg atcaatgcgg ggtgggtaaa acagtgccgg accacaacca ggacgggagt
      301 gagaacgttc ttgaccccga atttttttga tgagcctgct gtatgtattg ggaaggtgga
      361 aggggtcgat ttttgtcttg aggtggtctt tatagtacga tgggcgcccg ctgtgtcaaa
      421 cggctaaaac gccgggaggg gtcactatcc tgttcaagcc tacagcattg cttccacccg
      481 ttgtcaataa ggggcggccg aagcggcgtt cttgacaaaa tacagggcgt agtctatctt
      541 ttttttagga agccttcggg gcctgaaaat ggcagttttt tggaggcatc agggagacaa
      601 aagacaagat tttgatcaga agggagaaat ggcatggaac gatcaggggt aataaccatg
      661 aagggaaaac cgctcaccgt aatcggcccc gacgtcaggg caggtgaccg ggcgccggct
      721 ttttcggtgc ttgacgaaaa cctcaatagt gtcagcctgg cagactttgc gggaaaaaca
      781 aagctcataa gcgttacgcc gtccctcgac acaccggtgt gtgacctgca gctcaggcgc
      841 ttcaaccgcg aggcggcctc gatgccgcgg gatgtcgtcg tgctcaatat cagcatggac
      901 cttcccttcg ccgtcagccg cttctgttct acggcgggca tcgaccgggc caaggcgctt
      961 tcggaccacc gggatgcgtc gttcggcgcg gcctacggcg tgctcatcaa ggaactgcgg
     1021 cttctggcgc ggtccatctt catcatcgac aagaacgacg tcgtgaggta caaggagatc
     1081 gtacctgagc agtcgaacca tcctgattat gagaaggcgc tcagagcgct ggcggagatc
     1141 acttccgggg tcaaggccgc gtgacgattg aggaagggag ttgaagaagg gaaacgatgc
     1201 aaaaagaaag gcaggtgccc ttgcggggac ctgccttttt ttaagttcgg aaagcttact
     1261 gttttacgac gtttgctgct ttgggaccct tggggctgtc aacaacgtcg aacgtcactg
     1321 tctgaccttc ggaaagcgac ttgaagccgc tgtcctgaat gtcggaaaag tgcacgaaaa
     1381 catctccgcc gtcttctttg gtaatgaagc caaatccctt ggaatcattg aaccacttca
     1441 cggtgccttt tgccattaca ggttgaacct ccttctgtgg atattgaaga ttacggaaac
     1501 gtcataaaaa aagccgcaaa gcgtaaatct ttgcgacctc ttccaacaaa gacttccgaa
     1561 cttcagcact tatattgtca caagccgctg aaaaagtcaa gaattattta tgatactatc
     1621 gccttaagcg tgaacgtgag gggcaggaca atcgtttcct ctttgcctcc ctccgcggtt
     1681 tctagtatac ttgtgtccgg aaggcgaata aatctagaaa aacagcttgc cataacaaaa
     1741 agcacagaga tcacaaaaaa atgaaatgat aatctaaagc ctgatcgact tgattatttt
     1801 ttctttttca aggttagtat tttctctgtg attttcgtgc cttctgcggc caactcccgc
     1861 ttttagatta atacagcgat gaaaggaaca gcatggcccg accggaatca cgaacgcgca
     1921 aacagccgcg aacaaccttg aaactaggca ggacggtctg ggacttgagt ccgctcttcg
     1981 ccggcgatga cgaccctcgg atggagcaga agagaaagat cgtcagcagg aaaggccgtg
     2041 aattcatcaa cgcatggaag gaccggacgg attaccttga agacccggcg gtcctgctaa
     2101 gggcgttgga tcagtatgaa tcgtggaagc gcctgtacgg ctccgatgga gacgagggat
     2161 attacttctg gctccgcacc cagcaggacc agctcgaccc gaagctcaag gccaggttca
     2221 acaaaatcga ggagttcagc aagaagatcg aaaacgatat ccagttcttc tatctccgta
     2281 tcgccaagat aaagccggag gcccagcggc gctttctgga ggatgagggc ctggcgaaat
     2341 accggcacta cctcgaacgc atcttcgccg aggcgcgcta tcacctgagc gagcccgaag
     2401 aaaagatcct gaacctcaag tcctcgacgt cgtactcgaa ctggacgagg atgacttcgg
     2461 gattcctttc ccgggaggag cgggccgtga cgggcgagga cggcgtgaag aagatcaagc
     2521 ccttctcgga actcacgggg ctcatgaaca gcagggtcaa gcacgtgcgg gattcggccg
     2581 ccagggcgtt caacgacatc ctgcaaaagc acgtcgaagt cgcagaggcg gaactcaatt
     2641 ccgtgctgca gaacaagaag atcgacgacg agctgcgcgg cgcgccccgg cccgacaccc
     2701 tgaggcacct agcggacgac atcgagagcg acgtcgtcga tgccctgatc gattccgtct
     2761 cgcggcgctt cgacatccca tcgcggtact acgagctcaa ggccgccctg ctcagggtga
     2821 aacggcttca gtatcacgag cggaacgtgg aatacggcgt cgtcgccaag cggtatccct
     2881 atccgcaggc gataaacctc gtctggcgcg tgatgaacaa gctcgacggc cggttcggag
     2941 atatcctcac cgggttcgtg aagaacggcc agatcgacgc ctacccccgg aagggaaagg
     3001 acagcggcgc cttctgcatg caccatctcc tcgtccagcc cacctatatc ctcctgaacc
     3061 acaccgggaa actgcacgac gtcctgatga tcgcaca
//
LOCUS       DASSLD010000063         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1493253, whole genome shotgun sequence.
ACCESSION   DASSLD010000063 DASSLD010000000
VERSION     DASSLD010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493253"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VFK23_01180"
     CDS             complement(<1..384)
                     /locus_tag="VFK23_01180"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7317724.1"
                     /translation="MLDQKENPPELDAATGLPTKAIFFDRLTQALTLAKRKQRSLAVM
                     FISFDSLKLINDNLGQRFGDQLLKTVSERLVSCLRVSDTVARPGSNEFMVLLPEIETS
                     VNVPIVVDKIFRALGSSFVLEQHEIF"
     gene            630..1529
                     /gene="era"
                     /locus_tag="VFK23_01185"
     CDS             630..1529
                     /gene="era"
                     /locus_tag="VFK23_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET7317725.1"
                     /translation="MSEEKSFKSGFISIIGRPNVGKSTLLNALLGEKIAIISDKPQTT
                     RNKILGIVNAPGAQLVFMDTPGIHKPMHKMNEYMVQTALRTYNEVDVILMLVEATEAP
                     GGGDRFIIETLAKVKTPVFLLINKVDLIKKDALLPLIQQYSKLYPFAEIIPISALKND
                     LGDLVKAILTRLPQGPKYFPDDQLTDQPERFIVCEIVREKIFEHTKEEIPYSTAVMIE
                     EMKEEPDITRIHAVILVERDSQKGILIGKGGAMLKQIGTLARQDAEKLLGVKVFLKLY
                     VKVKKGWREDERMLRNIGIAGEE"
     gene            1611..2231
                     /locus_tag="VFK23_01190"
     CDS             1611..2231
                     /locus_tag="VFK23_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET7317726.1"
                     /translation="MHELINWLVETIGALGYPGIFTLMAMESSVIPIPSELVMPPAGY
                     LAQQGEMNMLIVILCGTVGSLVGAYANYFAAHYIGRPLVLKYGKYVWITEEKFAKVER
                     FFKDHGEVSTFIGRLLPVVRHLISIPAGLAGMNHLKFSLYTLLGAGLWVTVLSYIGYF
                     IGANRELIMQYSHQALIGAVVLSVGIIAVYIWNHRRKKQLDAGGRD"
     gene            complement(2277..>2544)
                     /locus_tag="VFK23_01195"
     CDS             complement(2277..>2544)
                     /locus_tag="VFK23_01195"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HET7317727.1"
                     /translation="ELSSGEDGYLSFTVGLGVVRTETRNLDTPVEFLLRGAYGRRIGR
                     FDISLGFTHFSNGKLFFRRLHPSWWNRGPNFGENFPTLQVGVLF"
BASE COUNT          579 a          726 c          731 g          508 t
ORIGIN      
        1 gaagatttcg tgctgttcca gaacaaaact cgaaccgagc gccctgaata tcttgtccac
       61 gacgatggga acgttcacgc tcgtctcgat ttcgggcagg aggaccatga actcgttgct
      121 gccgggccgc gccacggtgt cgctcacccg gagacaggag acaagccgtt ccgacaccgt
      181 cttgagcagc tggtccccga aacgctgtcc cagattgtcg ttgatcagct tgaggctgtc
      241 gaagctgatg aacatcacgg ccaatgatcg ttgtttgcgc tttgcgaggg ttaatgcctg
      301 ggtaagacgg tcgaaaaaga tggccttggt agggagcccg gtggcggcgt cgagttcggg
      361 gggattttct ttttggtcca gcacttcagc ctccggctgc gtaagaaaca atcccgggca
      421 ggttcgaatg gcaaagggga accaaagaat attactggtg tgaagcgtcg agttgccggc
      481 gtgcagaccg gttgttccgc gcccgctgca ttcgcctctt tttcgaatta tagtcatacc
      541 gtgcgtaaaa gtaaaggaaa tattcgcgcg ttgtccgcct ttcggctttt cagttgcaca
      601 cggcccctgt ttcgggtatc ctgtcaccca tgtccgaaga aaaaagcttc aagtccggat
      661 ttatttccat catcggcagg ccgaacgtcg gcaagtcgac gctcctgaac gccttgctgg
      721 gcgagaagat cgccatcatc tccgacaagc cccagaccac gcggaacaag atcctcggca
      781 tcgtgaacgc accgggcgct cagctggtgt tcatggacac gcccggcatc cataagccca
      841 tgcacaagat gaacgagtac atggtgcaga cggcgctccg gacgtacaac gaggtggacg
      901 tgatcctcat gctcgtcgag gctacggagg cccccggggg aggggaccgg ttcatcatcg
      961 agaccctcgc caaggtaaag acccctgtct ttcttctcat caacaaggtg gacctcatca
     1021 agaaagacgc actcctgccg cttatccagc agtacagcaa actgtatcct ttcgccgaga
     1081 tcatccccat atccgctctc aagaacgacc tcggcgacct cgtcaaagct atcctcacgc
     1141 ggctcccgca ggggccgaaa tattttcccg acgaccagct cacggaccag ccggagcggt
     1201 tcattgtttg cgagatcgta cgggagaaga tctttgagca cacaaaggaa gagattcctt
     1261 attccacggc agtcatgatc gaagagatga aggaagaacc cgacatcacg cgcattcacg
     1321 cggtgattct cgtcgaacgc gattcccaga agggcatcct gatcggcaag ggcggggcca
     1381 tgctgaagca gatcggcacc ctggcccggc aggacgccga gaagctgctc ggcgtcaagg
     1441 tctttctgaa actctacgtc aaggtcaaga agggctggcg cgaggacgag cgtatgctca
     1501 ggaatatcgg gatagcgggg gaggagtaaa acaaatgcgg atttcggaat gaggaatgcg
     1561 gaatgaaaac cggtaacccg taactcgcaa cccgcaacaa aggagtcgtc atgcacgaac
     1621 tgattaactg gctggtcgag accatcggcg cactgggcta tcccggtatc ttcacactca
     1681 tggcaatgga gagctccgtg atccccatcc cgagcgaact cgtcatgccg ccggccggtt
     1741 acctcgcgca gcagggcgag atgaacatgc tgatcgttat cctctgcggt acggtaggga
     1801 gccttgtcgg cgcctatgcc aactactttg ccgcgcacta tatcggaaga ccgctggtcc
     1861 tcaagtacgg gaaatacgtc tggatcacgg aggaaaaatt cgcgaaggtc gagaggttct
     1921 tcaaggacca cggcgaggtg tccacgttca tcgggaggct gctcccggtc gtccggcacc
     1981 tcatctcgat cccggcgggc ctcgccggta tgaaccacct gaagttctcg ctctacaccc
     2041 tgctcggcgc cgggctctgg gtcaccgtgc tttcctacat cggctacttc atcggcgcga
     2101 accgcgagct gatcatgcag tattcgcatc aggccctgat cggcgcggtg gttctgagcg
     2161 tcggcatcat tgccgtgtac atctggaacc accggaggaa gaagcagttg gatgccggcg
     2221 gcagggattg acgtatgtcg atgcccccgg aaatccgaag tatgccgtat gcggggtcag
     2281 aacagaacgc ccacctggag cgtcggaaaa ttctcgccga aattggggcc gcgattccac
     2341 caggaaggat ggaggcggcg aaaaaacaac tttccgttcg aaaagtgcgt gaaacccaaa
     2401 gaaatgtcga atctcccgat ccggcgaccg tatgcgcccc tgagtaggaa ttcgacgggc
     2461 gtgtcgaggt ttctcgtctc tgtgcgcacc acgccgagcc ctaccgtgaa actcaagtag
     2521 ccgtcctctc cggacgacag ttca
//
LOCUS       DASSLD010000064         4186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_319344,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000064 DASSLD010000000
VERSION     DASSLD010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319344"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1187)
                     /locus_tag="VFK23_01200"
     CDS             complement(81..1187)
                     /locus_tag="VFK23_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7317728.1"
                     /translation="MIKTALSHIKGVRAYVLAGLLAVISAIHYAAGGASAGILHGLLG
                     HLYIVPIILGAYWYGAKGGVSVSIASSALFSPHLFLHWHDPFLDIYNYVELFLFLLIG
                     GVTGVLSEMERNQRMRHEEAMRRLDESHRKLKEQTDILFQTEEQLRRADRLSALGELS
                     AGMAHEIRNPLGAIKGAVEILKDDYSPEEAKYEFIQILLKETDRLNCIVQEFLGFAQP
                     KQPEYSEADVNESLTAVLTLTAQEARKAGIKIVSRLDPSIGKRSLDAGLLKQAFLNLV
                     LNAIQAMPTGGSLTVESSLRDGMIVVNVTDTGTGISDENRRKLFSPFFTTKKNGTGLG
                     LAITYRIIENHHGRIDVVSELGKGTTFTVKIPVN"
     gene            1522..1743
                     /locus_tag="VFK23_01205"
     CDS             1522..1743
                     /locus_tag="VFK23_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317729.1"
                     /translation="MKKIIALFTAMAFALTLGVAFAQEQTAPAAAPEKKEAPAKKKKA
                     AKKKKAAKKEEAAKKEEAAPAAPAAPAAK"
     gene            complement(1930..2799)
                     /locus_tag="VFK23_01210"
     CDS             complement(1930..2799)
                     /locus_tag="VFK23_01210"
                     /inference="COORDINATES: protein motif:HMM:NF025428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HET7317730.1"
                     /translation="MKRFVSRLLSHMAAAGAVLCLMGADGQMKSATAISGAETTTNGP
                     ASGLTSSITGSAAGKTSNSLSAGATGSVASKPTHSLTTGATGSAAVTSKPTVTLSPYA
                     EELVKILKFDRQVAILVKEVTSEHIQRLVGYDENDYQIIAPGIAVAVPEDKTDRILAQ
                     LRRKLVPLRYLPFVVEMNEGLKRDKIGIIKGTDQYEILRIMHTNGDQYDISNEDLIER
                     LKEWEKITPFTIIGADGDWVELEFRTLPKDLKAFAEEVYEFSPEAVEQGAGSVEELMK
                     EIKKTNRLFLSWD"
     gene            complement(2987..3622)
                     /gene="nth"
                     /locus_tag="VFK23_01215"
     CDS             complement(2987..3622)
                     /gene="nth"
                     /locus_tag="VFK23_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET7317731.1"
                     /translation="MDKKKQVAEILKRLKKEYSGTPQTALNFTTPWQLLVATILSAQT
                     TDVLVNKVTEPLFKKYRTIEDIAATTPEKLAMDVASVNFYRNKAKSINKAAKMILEQF
                     GGKVPQTMENLVALPGVARKTANIVLWSAFGINEGIAVDTHVKRLAFRLGLTKQEDPV
                     KIEADLMELAPRKEWGNLSHLLIFHGRKICQARRPNHAACVLFDICPSRNI"
     gene            complement(3776..>4186)
                     /locus_tag="VFK23_01220"
     CDS             complement(3776..>4186)
                     /locus_tag="VFK23_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013887125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET7317732.1"
                     /translation="GATAGAVCAFLSARYLLGERLQQKYRGQLAKFNGEMERNGVSYL
                     LTLRLIAVFPFFLVNFLAGLTKVTLKTFLWTTAVGIIPGSAVFAYAGRQLGSVNSPAD
                     IISKKVIAALAALGFITLAPAVWKRIRKPKTQSQ"
BASE COUNT          862 a         1186 c         1140 g          998 t
ORIGIN      
        1 acacgaaggc acgaagaaaa gcggaaacac cctgccagat tgactttctt tgtgtcctag
       61 tgccttcgtg gtaaaagatt ttaattcacc ggtatcttca ccgtgaacgt cgtgcccttg
      121 cccaactcgc tcacgacatc gatccttccg tgatggtttt cgatgatccg gtatgttatg
      181 gcgagtccca ggccagtccc gtttttcttg gtcgtgaaga aggggctgaa aagcttcctg
      241 cggttttcat ccgagatgcc cgttccggtg tccgtaacat tgaccacgat catcccgtct
      301 cgcagactgc tttcaacagt aagggagcct cccgtaggca tggcctggat ggcgttcagg
      361 acgaggttca ggaacgcctg tttgagcagt cccgcatcaa ggctgcgttt cccgatggac
      421 ggatccagtc gactcacgat ctttatcccg gccttgcgtg cctcctgagc cgtgagcgta
      481 agcaccgccg tgagcgattc gttcacgtcc gcctcgctgt attcgggctg cttgggctgg
      541 gcgaacccga gaaactcctg tacgatgcag ttcagccggt ctgtctcctt cagcagaatc
      601 tggatgaact cgtacttcgc ctcctcgggc gaatagtcgt ctttcaggat ctctacggcg
      661 cccttgatcg cgcccagggg atttctgatc tcgtgcgcca tgcccgcaga cagctcccca
      721 agcgcggaca gccggtctgc gcgccgcagc tgttcttcag tctggaatag gatgtctgtc
      781 tgctccttca gcttccggtg ggactcgtcg aggcggcgca tggcctcttc atggcgcatc
      841 cgctggttgc gttccatctc gctcagtacg ccggtgacac cgcctatcag gagaaagaga
      901 aacagctcga cgtagttata gatgtccaga aaagggtcgt gccagtgcag gaaaaggtgc
      961 ggagagaaca aggccgatga agcgatggaa acggaaacgc cgcccttggc cccgtaccag
     1021 tacgcgccca ggatgatcgg gacaatgtag agatgaccca gaagaccgtg caggatgcct
     1081 gccgaagccc caccggcagc atagtggatt gccgagatga ccgcgaggag gcccgcgagc
     1141 acgtacgccc tgacgccctt gatatggctg agtgctgttt tgatcatacg cggcatttgg
     1201 aatggttcac aagaggtcct cgcggcctcc cgtgctgcgc caattaaatc taaccgaatc
     1261 cctcctgttt gtcaagaaat gactgatttt tcaggggttc cgccatcaag ccggcagagt
     1321 ttccggacca tcccgcggag tcgacggtcc atctttacat cttatagttt attctattcg
     1381 caacctttcc gataggagtc gtctcatata ccagcgtatc agaaaaaggg cccgggatgc
     1441 cctctattgt tcttgacatc aattatcaaa cctgttataa atatccgcca gattccataa
     1501 aaggaaggga ggtgaaacca gatgaagaag attatcgccc ttttcacggc catggcgttt
     1561 gccctgaccc tcggcgttgc tttcgcccag gagcagacgg ccccggcagc agcaccggag
     1621 aagaaggaag ctccggcaaa gaagaaaaag gctgcaaaga agaaaaaggc tgcaaagaag
     1681 gaagaggctg caaagaagga agaggcagcc ccggcagctc cggcagcccc ggcagcaaag
     1741 tagtttctcc tataccgcta atttattatt cggaagcgga aaaaaatgta attttgaatg
     1801 gaacaggtgt ttatgcggca ggcgaataaa ttcgcctgcc gttttttttt cgtcattcac
     1861 ccgcggtata tcttcttacg gcattttctc acagcgaact tttttgtcat caacgtatga
     1921 cagataaaat taatcccacg agaggaataa acggttcgtt ttcttgatct ccttcatcag
     1981 ttcttcgacg ctccccgccc cctgctcgac ggcctcgggg ctgaattcgt acacttcctc
     2041 agcaaaggct ttcaggtctt tcggcagcgt cctgaactcc agttccaccc agtcgccgtc
     2101 cgccccgatg atggtgaagg gcgtgatctt ctcccactcc ttcaaccgtt cgatcaggtc
     2161 ctcattggaa atgtcgtatt gatctccgtt ggtatgcatg atgcggagga tctcgtactg
     2221 gtccgtcccc ttgatgatgc ctatcttgtc cctcttgagc ccttcattca tctccacgac
     2281 gaagggcagg tagcggagcg gaaccagttt cctgcggagc tgggcaagga tgcggtccgt
     2341 cttatcttcc ggcaccgcaa cagcaatgcc gggggcgatg atctggtagt cgttttcatc
     2401 gtaaccgacc agccgctgga tgtgctcgct cgttacctcc ttcaccagga tggcgacctg
     2461 ccggtcgaat ttgagaattt tgaccagttc ctcggcatac ggcgaaagcg tcacggtcgg
     2521 tttgctcgtg acggccgcgc tgcctgtcgc gccggttgtc aggctgtgcg tgggtttgct
     2581 ggcgacgctg cccgtcgcgc ctgccgacag actgttcgac gtttttccgg ccgcgctccc
     2641 cgtaatgctg ctggtaagac cgcttgccgg accgtttgtc gtcgtctctg ccccgctgat
     2701 ggccgtcgcg ctcttcatct ggccgtccgc ccccatcaag cacagaacgg cacctgctgc
     2761 tgccatgtgg gacagcagcc tgcttacgaa tctcttcata gcgtgggaga ttctatcata
     2821 gcggggagag gaaaacaaca ggatatgccg cccctgcgaa gggtagaacc gaaacggcga
     2881 ttgggtgatc gggggagtgg gcgagaagga aggaatgaaa cgggctgcgg tcctttgttt
     2941 ttttcgccga ttctccccct cgcccccctt tccccttcga tgtctattaa atgttcctcg
     3001 acgggcagat atcgaacagt acgcacgcgg cgtggttcgg ccttctggcc tggcagatct
     3061 tcctgccgtg aaagatcagc aggtgcgaca ggttccccca ctccttgcgc ggcgcgagct
     3121 ccatgagatc ggcctcgatt ttgaccgggt cttcctgttt cgtaagcccc agacggaacg
     3181 cgagtctctt gacatgggtg tccacggcaa taccctcgtt gatcccgaag gcggaccaca
     3241 atacgatgtt cgccgtcttc cgggccacgc cgggaagggc aacgaggttc tccatggtct
     3301 gcgggacctt gccgccgaac tgttccaaga tcatctttgc cgccttgttg atgctcttcg
     3361 ccttgttccg gtaaaaattg accgaggcca cgtccatggc tagcttttcc ggtgtggttg
     3421 ccgcaatatc ctcgatagtc ctgtattttt tgaacagcgg ctcggtcacc ttgttgacaa
     3481 gaacatcggt ggtctgcgcc gacaggattg tcgcgaccag gagttgccag ggggtcgtaa
     3541 aattcagggc cgtctgcggc gttccggagt attccttttt gagtcttttc agtatttccg
     3601 caacctgttt cttcttgtcc atgggacctc cttgcaggta taaatttcaa atcccaaata
     3661 gcaaattcca gaattcaaaa tcccaattac cgaaatgttt caatatgatc attggtgctt
     3721 ggagcttatt tgtaatttgg agcttgttct gtcgtgctta ccctcggaaa tatcgttatt
     3781 gagactgcgt tttcggtttt cggatgcgtt tccagacagc cggcgccagc gtaataaacc
     3841 cgagtgcggc cagggcggca atgaccttct tcgaaatgat gtcagcgggg gaattgacgg
     3901 acccgagttg ccggccggca taggcaaata ctgcgctgcc cgggatgatg ccgaccgccg
     3961 tcgtccagag aaaggtcttg agcgtgacct tcgtgaggcc ggccagaaaa ttaacgagga
     4021 agaagggaaa caccgcaatc aggcgcagcg tgagcagata gctgacgccg ttccgctcca
     4081 tctcgccgtt gaacttcgcg agctggcccc tgtacttctg ctgcagccgt tcccccagca
     4141 ggtaccgggc cgaaaggaac gcgcacaccg cccccgctgt tgcgcc
//
LOCUS       DASSLD010000065         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_938487,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000065 DASSLD010000000
VERSION     DASSLD010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938487"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            431..778
                     /locus_tag="VFK23_01225"
     CDS             431..778
                     /locus_tag="VFK23_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317733.1"
                     /translation="MIFSGFNSAVPIPPAPILIIFSVLFIAQFVVPAYFGIQYLGRLP
                     VDENSHVRPAIRPGQAMRAPAGSREPGQSMPVKSYKEAPVQTLEVIRKGGSSEERRGG
                     CLHDHLFCSAHIL"
     gene            744..>2850
                     /locus_tag="VFK23_01230"
     CDS             744..>2850
                     /locus_tag="VFK23_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317734.1"
                     /translation="MIICFALLTSCSGGGGGTTSTTTGGDPGGTGNTGDNLDGSTADG
                     VNTLRALIDDVPEASSGSLVILDGSRSSDPNGGALIYSWSQVSGPTVTHSDAGKNTLY
                     FTAPAGTAELVFELSVTNSGGLSSKATATVAVTQNPSSPPPTSIALIDDAVRNGTITA
                     EQGLVYKVFAVFNDGSLPAEYRGSGLGQTSGTRFMAELAEKYDSLSPEAKAQVYPYLL
                     PPYVANSWYDLAQQKGMLSGKIRSAFARAAAPAAPWKWVTNGKVKVWYQDGQVNYLDG
                     SSVALADLGQGVLDAVNGKIWGKLQELMKKEPLQDAGASLLPLPSPDYGAIPGPIDMD
                     GALDIILCSGMNASGYTNPYHAVQPTPVFITIDYTMWKLGDERTPGLVQIVAHEMMHA
                     WQFSYVLKDDPLTYRWLMEATAAWAEDYVYPDANSENRYAAWYLDTVPLTLDNETNFR
                     QYGAYTAFSYWTHSDRGGPPDIVRQAWEAAATKSSLDAPDSMNPLPEPMNSTAPHYRT
                     SFFERYWGDFLVHAWNRGANGFFFSKDKLTKGSRARANTPIAVELAGAEDEVYYLDDL
                     DKSGIIELPYLSGRYYHFVFTNDNDRTVLFFDGLRSSLTLTGDATGAVITGVPLIPDP
                     NDPSADPAEGATWRLLAKIDGQWKEWQPPQMSELTGVPFCRDAKAERLEELVAILANS
                     SADKANVVRPKGELPPLLLA"
BASE COUNT          606 a          847 c          823 g          574 t
ORIGIN      
        1 tggcagcatg cgcagctggc ttttgatcac cagaaaatcc tgcgcgacgc gtggcgaatg
       61 tataagcaag gttaaggacc attcaaattc cgaattccac attccgcatt ttgagatgag
      121 gggcatcgtg gttcagtttg aattagaaga ctgttcgtcg cggcaggtcc gctctgcgag
      181 taccgttacg ctgaatacat tctctgacag ccggcttgtc ggaacccgta ccgcttctcg
      241 tcggttttta ggcgggtctc gttgattttt cgtcaattct ccgacatccc gaaaataccc
      301 aattgagttc gttgacaaaa aaccttcata tccatataat agacgtaccg caacgatagc
      361 ctgcctttag ccacgatagc ttctccatcc tatctcaaac gtgttctaac agtgagatgg
      421 agcaagcacc atgatattct ccggtttcaa ttccgctgtt ccgattcccc ctgctccaat
      481 cctcatcatc ttttctgttc tcttcatagc gcagttcgtc gtgccggcgt atttcgggat
      541 tcagtatctc ggacgcttgc cggtcgatga gaacagccat gtgcggcccg caatacggcc
      601 ggggcaggcg atgcgtgctc ccgccggcag cagagagccg gggcaatcga tgccggtgaa
      661 aagctacaaa gaagcgccgg tgcaaaccct ggaagtgatt cgcaaaggag ggtcttctga
      721 agaacgtagg gggggttgtt tgcatgatca tttgttttgc tctgctcaca tcctgtagcg
      781 gcggtggcgg cggtaccacc agcacgacca caggaggtga tcccggcggt accggcaaca
      841 caggagataa tctcgacgga agcactgctg acggcgtaaa tacgctgcga gcgctgatcg
      901 atgacgtccc ggaggcaagt tccggaagtc tggtcattct ggacggttcc cgctcttcgg
      961 atcccaatgg cggcgctctg atctattcct ggtcgcaagt gagcgggccc accgttacac
     1021 actctgatgc cggcaagaac acattgtatt tcaccgctcc ggcgggaacg gccgaactgg
     1081 tttttgaact ctcggtcacc aattcaggcg gtctgtccag caaggcaaca gccaccgttg
     1141 ccgttacgca gaatccgtcg tcaccgccgc ccaccagtat cgccctcata gacgatgctg
     1201 tccggaacgg aacgatcact gccgagcagg ggcttgtcta taaggtcttt gccgtcttta
     1261 acgacggcag cctcccggca gaataccggg ggagcggtct cggtcagacg agcggaacgc
     1321 ggttcatggc cgaactcgcg gagaagtacg actcgctctc gccggaggcc aaggcccagg
     1381 tctaccccta tctccttccg ccttatgtcg cgaacagctg gtacgatctg gcgcagcaga
     1441 aaggaatgct gtccgggaaa ataagaagtg ctttcgcccg cgctgcagca cctgccgccc
     1501 cctggaagtg ggtaacaaac gggaaagtca aggtctggta ccaggacggg caagtgaact
     1561 atcttgatgg ttcctccgtg gcattggccg atcttggaca aggcgtgctc gatgcggtaa
     1621 acggaaagat ctggggcaaa ctgcaggagc tgatgaagaa ggagccgctc caggatgccg
     1681 gtgcctcgct gctcccgctc ccaagcccgg actacggggc gataccgggc ccgatcgata
     1741 tggatggcgc cctggacatc atcctgtgtt ccgggatgaa cgcttccggc tatacgaacc
     1801 cgtatcacgc ggtgcagccg acccccgttt tcataacgat cgactatacc atgtggaagc
     1861 tgggcgacga aaggacgccc gggctcgtcc agattgtggc ccatgagatg atgcacgcgt
     1921 ggcagttcag ctacgtgctg aaggatgacc ccctcaccta tcgctggctc atggaggcaa
     1981 ccgcggcctg ggcggaggac tacgtctacc ccgatgcgaa cagcgagaac cgctacgcgg
     2041 cctggtacct ggacacagtc cccctgactc tcgacaacga gacaaacttc cggcagtacg
     2101 gcgcctacac cgccttctca tactggacgc acagcgatcg gggagggccg cccgatatcg
     2161 tgcggcaggc atgggaggct gcggcaacga agagcagcct cgacgctccg gactcgatga
     2221 acccgctccc ggaacccatg aactcgaccg cgccccacta ccggacatcc ttcttcgaac
     2281 gctactgggg cgacttcctc gtgcatgcct ggaaccgggg ggcgaacggg ttcttctttt
     2341 cgaaggacaa gcttaccaag ggcagcaggg caagggcgaa cacgcccatc gcagtcgaac
     2401 ttgcgggcgc ggaggacgag gtttattatc tcgacgacct ggataaatcc ggcataatcg
     2461 agctcccgta cctgtcaggc cggtattacc acttcgtgtt cacgaacgac aacgatcgta
     2521 cggtgctgtt ctttgacggc cttcgatcgt ctctgacctt gacgggtgac gccacgggcg
     2581 cggtcataac aggagtcccc ctaattcccg atccgaacga tccctccgcc gacccggccg
     2641 agggcgccac ctggcggctg ctcgcgaaaa tcgatggaca gtggaaggag tggcagccgc
     2701 cgcagatgtc cgagctcacg ggggtgcctt tctgccggga cgccaaagcc gagaggctgg
     2761 aagagctcgt cgccatcctg gcgaacagct ccgcggataa ggccaacgtg gtaaggccga
     2821 agggtgaact ccccccgctc ctgcttgcgt
//
LOCUS       DASSLD010000066         3082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1427680, whole genome shotgun sequence.
ACCESSION   DASSLD010000066 DASSLD010000000
VERSION     DASSLD010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427680"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /gene="trpD"
                     /locus_tag="VFK23_01235"
     CDS             <1..700
                     /gene="trpD"
                     /locus_tag="VFK23_01235"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET7317735.1"
                     /translation="VAKHGNRSVSSKSGSADVLQALGVNIEVPAHRVEECMREVGIGF
                     LFAPLMHLAMKYAIGPRREVGIRTIFNVLGPLTNPARVKSQIMGVYAADMTAPLAQAL
                     GNLGARHAFVVHGMDGLDEITITDRTRISEFKSGTVKDYFIHPSDFGLPAGKAEDLKG
                     GDAKDNAAITVEILKGRKGARRDVVLLNAAAGLVASGRAADIRGGVKLAEESIDSGAA
                     LKKLEELKAFTNKT"
     gene            858..1637
                     /gene="trpC"
                     /locus_tag="VFK23_01240"
     CDS             858..1637
                     /gene="trpC"
                     /locus_tag="VFK23_01240"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET7317736.1"
                     /translation="MILDDIIANKKEELAELKRRVSPADIKAKAADAGPVRGFAAALS
                     AGKGVRLIAEIKKASPSKGVIREDFDPVAIARVYAGSGASCISVLTEQKFFQGKIEYL
                     DSVRKAQGLPLLRKDFIVDEYQIFEARAAGADAVLLIAACLEKRQIEDFLGIAAGLGL
                     DALVESHTYRELDKSLLAGAVLVGINNRDLSTFAVSLQTTLDLLKDIPDDRTVVSESG
                     IGTRDDVVKLENAGVDAILAGESLMREKDIVKKVKELLGKE"
     gene            1723..2343
                     /locus_tag="VFK23_01245"
     CDS             1723..2343
                     /locus_tag="VFK23_01245"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET7317737.1"
                     /translation="MIKIKICGITNLEDALAAAAAGADALGFNFYKKSPRFIEPAKAA
                     EIIDQLPPFIVPVGVFVNEREEKIREIQALTCMQAIQLHGDESPEFCQRLYGRIIKAF
                     QVKDKESLQNMAHYRVAAYLLDSYREGVRGGTGVTFDWHLAVVAKTFGKVILAGGLTP
                     ENVAEAVKLVQPYGVDVAGGVEREKGIKDHAKLRKFITEVRRASRP"
     gene            2340..>3082
                     /gene="trpB"
                     /locus_tag="VFK23_01250"
     CDS             2340..>3082
                     /gene="trpB"
                     /locus_tag="VFK23_01250"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET7317738.1"
                     /translation="MIIPDKKGHFGIYGGKFAPETLMPALAELEAAYLAAKKDKAFQA
                     ELDFYFREFIGRPTPLYFAKRLTEHLGGAKIYLKREDLCHTGAHKINNSLAQVLLARR
                     MGKTRLVAETGAGQHGVATATAAAMFGLDCEVYMGTDDVARQSLNVFRMKLLGTTVRP
                     VDIGSKTLKDAINEAMRDWITNVANTHYVLGTVFGPHPFPVMVRDFQSVIGREAKKQI
                     MKFEGRLPDALIACVGGGSNSMGLFHEFI"
BASE COUNT          675 a          881 c          981 g          545 t
ORIGIN      
        1 cgtggcaaag cacggaaacc gctcggtctc gagcaagagc gggagcgccg acgtgctcca
       61 ggccctgggc gtcaacatcg aggtccccgc ccatcgcgtc gaagaatgca tgagggaggt
      121 gggcatcggc tttctctttg ctcccctgat gcacctggcc atgaagtacg ccatcgggcc
      181 acggcgggag gtcggcatcc gcaccatctt caacgtgctg gggcccttga ccaatccggc
      241 aagggtcaag tcacaaatca tgggcgtcta cgcggcagac atgaccgccc cccttgcgca
      301 ggcgctcgga aatctcggcg cacgtcacgc cttcgtggtg cacggcatgg acggtctcga
      361 cgaaatcacg atcacggaca ggaccaggat ctcggagttc aagagcggga cggtgaagga
      421 ctacttcatc catccgtcgg atttcggttt gcccgcaggc aaggccgaag acctcaaggg
      481 gggtgacgct aaggacaacg cggccatcac ggtggaaatc ctcaaaggcc ggaaaggtgc
      541 gcgccgcgac gtcgtgctcc tgaatgcagc ggcgggtctc gtcgcttcgg gcagggccgc
      601 ggacatccgg ggaggcgtca aactggcgga agaatccatc gattccggcg cggcgctcaa
      661 gaaactggaa gagctgaagg cgtttacgaa taaaacttaa aaaaacaatt ttcgcgcaga
      721 ggcgcagagc tcgcagagaa aggcagtctt ttgggtttaa aaccagaatc taaagatttt
      781 ctttgcgtcc ttagcgtctt tgcgagagac cgattttgac tttacgcttt cattgatttg
      841 gaacattgcc ttgctaaatg atcttagacg acatcattgc gaacaaaaaa gaggagttgg
      901 ccgagctcaa gcgccgcgtt tcccccgccg acattaaggc gaaggcggct gatgccggac
      961 cggtgagggg ttttgcggcc gcgctttcgg cggggaaggg cgtcaggctc attgcagaga
     1021 tcaagaaggc ctcgccgtcc aagggtgtga tccgcgagga tttcgacccc gtggcaatcg
     1081 cgcgggtcta cgccgggtcg ggcgcgtcat gcatttcggt cctgacggag cagaagtttt
     1141 ttcagggaaa gatcgagtat ctcgactccg ttcgaaaggc ccaggggctg ccgctcctgc
     1201 gcaaggactt catcgtcgac gagtaccaga tcttcgaggc gcgggccgcg ggcgccgacg
     1261 cggtccttct gatcgcggcc tgcctggaga aacggcagat cgaggatttt ctcggcatcg
     1321 ccgccgggct cggtctcgat gcgctggtgg agtcccacac gtaccgggaa ctggacaagt
     1381 cgctccttgc cggtgcggtg ctggtgggga tcaacaaccg ggacctgtcg acctttgcgg
     1441 taagccttca gacgacgctc gatcttctga aggacatccc cgatgaccgt acggtcgtga
     1501 gcgagagcgg cattgggacg cgggacgacg tcgtgaaact ggagaacgcg ggcgtggacg
     1561 cgatcctcgc cggtgagagc ctgatgaggg agaaggacat cgtgaaaaag gtgaaggaac
     1621 ttttaggaaa ggaatgaggt aaccacagat aagcacggta gggcaaaagc ctggatttgc
     1681 gcttgtcgtt catccgtgtt catccgcggt tatacaaaac ctatgatcaa aatcaaaatc
     1741 tgcggcatca cgaacctgga agacgcgctt gcggcggcag cggccggggc cgacgcactc
     1801 ggattcaact tctacaagaa gagcccgcgc ttcatcgaac cggccaaggc ggccgagatc
     1861 atcgaccaac tgccgccttt catcgtgccc gtcggcgtct tcgtgaacga gcgcgaggag
     1921 aagatccggg agatccaggc cctgacctgc atgcaggcga tccaacttca cggagacgag
     1981 agccctgaat tctgccagcg attgtacggc cgcatcatca aggcgtttca ggtcaaggac
     2041 aaggaaagcc tccaaaacat ggcgcactac cgggtggctg cgtacttgct cgacagctac
     2101 agggaaggag tgcggggcgg gacgggtgtt acgttcgact ggcatctcgc cgtggtcgcc
     2161 aagaccttcg gcaaggtgat cctggccggc gggctcactc ccgagaacgt ggccgaagcc
     2221 gtgaagctcg tccagcccta cggcgtggac gtggccggcg gcgtggaaag ggagaaggga
     2281 atcaaggacc acgcgaagct caggaaattc ataaccgagg tccgaagggc gtcgcgacca
     2341 tgatcatacc ggataaaaaa ggacatttcg gcatatacgg cggaaagttc gcgcccgaga
     2401 cgctgatgcc ggcgcttgcc gaactcgagg ccgcatacct cgcggcaaaa aaggacaagg
     2461 cgtttcaggc cgagctcgat ttctacttcc gcgagttcat cggccgaccc acgcccctct
     2521 atttcgcaaa acgcctgacg gaacacctcg gcggcgcgaa gatctacctc aagcgcgaag
     2581 acctctgcca tacgggcgcc cataagatca acaactccct ggcgcaggtc ctgcttgccc
     2641 ggcgcatggg caagacccgt ctggtcgcag agacgggggc cggccagcac ggcgtggcca
     2701 cggccacggc ggcggccatg ttcggactcg actgcgaggt ctacatgggc accgacgacg
     2761 tagcccgcca gtcattgaac gtcttccgca tgaagctcct gggcacaacc gtgcgtccgg
     2821 tggacatcgg gagcaagacg ctcaaggacg ccatcaacga ggccatgcgg gactggatca
     2881 cgaacgtggc gaacacgcac tacgtgctgg gcacggtctt cggcccccat cccttcccgg
     2941 tgatggtgcg cgatttccag tccgtgatcg gccgggaggc gaaaaagcag atcatgaagt
     3001 tcgagggcag gctgcccgac gccctgatcg cctgtgtggg aggcgggtcg aactcaatgg
     3061 ggctgttcca tgagtttatc ga
//
LOCUS       DASSLD010000067         2848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1004083, whole genome shotgun sequence.
ACCESSION   DASSLD010000067 DASSLD010000000
VERSION     DASSLD010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004083"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1581)
                     /gene="selB"
                     /locus_tag="VFK23_01255"
     CDS             complement(<1..1581)
                     /gene="selB"
                     /locus_tag="VFK23_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HET7317739.1"
                     /translation="MKYIILGTAGHIDHGKSSLVKALTGIDPDRLKEEKERGITLDLG
                     FASLDLPGGNRLGIVDVPGHEGLIKNMLAGVGGIDIVMLVIAADEGIMPQTREHLAIC
                     NLLHVKKGIIALTKTDMVDKDWLALVTDEVRDFVKGTFLEKSAIVPVSSKTGENLPAL
                     VQELAKLSNEVSPKSPNGILRLPIDRVFTMKGFGTVITGTLLSGTVSIEQEVDILPKG
                     IKTKVRGIQSHNKAVQRAVAGQRTAVNLQGVEKDQLSRGDTIVSAGFFSTTKTLDAKL
                     DLLKQAPRGLKTGSRVRFYNTTQEAIGRLTILGSNELAPGEEAFVQIRLEHPVIVQHG
                     DRYILRFYSPMETLGGGVVLNPHTRRHKQAAMKESIRALGVLERGPIEEKLPLFISGK
                     GLAGMEEAAIIGDMAGDKQEIQSALAALVQKKTILRVDNLYVHASHVAELEKKALDLV
                     KQYHKDNPLKPGLDKEELKGALKMRLSPKVLNAALDGLVRKKQLEMEGSKLRVPGFKA
                     AGGKVQDEIKNRIVEAIKK"
     gene            complement(1702..>2848)
                     /gene="selA"
                     /locus_tag="VFK23_01260"
     CDS             complement(1702..>2848)
                     /gene="selA"
                     /locus_tag="VFK23_01260"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HET7317740.1"
                     /translation="ATGVVVHTNLGRSILSEAAIRRVMEVSRSYSNLEYDIAAGERGK
                     RHVHVENMLKRLTGVESATAVNNNAAAVLLCLNTLARGREVIVSRGELVEIGGSFRVP
                     EVMERSGAVLREVGATNKTHLKDYEKAINENTALLLKVHTSNYKIVGFTKEVSSEELV
                     SLGKKHNIPVMWDLGSGSFIDLRSYGAGEEPTVRQAVDSGVDVLTFSGDKLLGGPQAG
                     MVLGRKKYLDPIRSNPLTRALRLDKMTLAALDATLGQYLDMDKAVRDIPTLWMLTQPL
                     SEIERKAELLASGIRHQASAGLTVTIQDDRSQSGGGALPTGNFPTKVVCIRHDRLSAN
                     QIESRLRTGKPHIIARIKEGMVVFDPRTLNDEEIGKIMEAMEKIITS"
BASE COUNT          477 a          831 c          885 g          655 t
ORIGIN      
        1 tttcttgatc gcttccacga tcctgttctt gatctcgtcc tggaccttgc cgcccgccgc
       61 cttgaacccc gggaccctga gtttggaacc ctccatctcc aattgtttct tcctgacgag
      121 tccgtcaagg gccgcattca ggaccttcgg ggaaaggcgc atcttgagcg cgcccttgag
      181 ttcctccttg tccaggcccg gtttgagcgg attgtccttg tggtactgct tcacgagatc
      241 gagcgccttt ttttcaagct ccgcgacgtg tgaggcgtgc acgtagaggt tgtcaacgcg
      301 caggatcgtc tttttctgga caagcgcggc gagggccgac tgtatctcct gtttgtcgcc
      361 cgccatgtcg ccgatgatcg cggcctcctc catgccggcc aggcccttgc ccgagatgaa
      421 gagcgggagc ttttcctcaa tcggccctct ttccaggacg cccagtgccc ggatcgactc
      481 cttcatggcg gcctgcttgt gacgcctcgt gtgcggattg aggaccacgc cgccgcccag
      541 ggtctccatg ggagaataga accggaggat gtaccggtcc ccgtgctgga cgatcacggg
      601 atgttcgagc ctgatctgaa caaaggcctc ctcaccgggg gcgagttcat tcgatccgag
      661 gatggtgagc cttccgatgg cctcctgcgt cgtgttgtaa aagcggacgc gcgagccggt
      721 cttcaggccc cggggagcct gcttcaggag gtcgagcttc gcgtccagcg ttttcgtggt
      781 ggagaagaaa cccgcgctca cgatcgtgtc gccccgtgaa agctggtcct tctccactcc
      841 ctggaggttc acggcagtac gttgtcccgc cacggcgcgc tgcaccgcct tgttgtgcga
      901 ctggatcccc cggacctttg ttttgatgcc cttcggcagg atatcgacct cctgctcgat
      961 gctgaccgtg ccggacagca gggtgccggt gatgaccgtg ccgaagccct tcatcgtgaa
     1021 gacgcggtcg atgggaaggc gcaggatgcc gttcggcgac ttgggggaga cctcgttcga
     1081 gagcttcgcc agttcctgga ccagggcggg caggttctcc ccagttttgg acgacacggg
     1141 gacgatggcg gacttttcga ggaaagtgcc cttgacgaaa tcgcggacct cgtcggtcac
     1201 cagtgcgagc cagtccttgt cgaccatgtc cgtcttggtc agggcgatga tgcccttctt
     1261 cacatgaagg aggttgcaga tggcgaggtg ctcccgggtc tggggcatga tgccctcgtc
     1321 ggcggcaatc acgagcatga cgatgtcgat gccgccgacg ccggcgagca tgttcttgat
     1381 gagcccttca tgacccggga cgtcgacgat accgaggcgg ttgcctcccg gcaggtcgag
     1441 ggaagcaaag cccaaatcca gcgtgatccc ccgttctttt tcctccttca gacggtccgg
     1501 gtcgatgccc gtgagcgctt tgacgaggct ggacttgccg tggtcgatat gtccggctgt
     1561 gccgaggatg atgtatttca tggaatcatt ttagccacag aggtcacaga ggacacagag
     1621 aacttcctaa aagattggac atataacaat tgtaattttt ttgtctttct ctgtgaactc
     1681 tgtgttctct gtggcaagaa gttaagatgt gattattttt tccatcgctt ccatgatttt
     1741 cccgatctct tcgtcgttca gggtgcgcgg gtcgaagacg accatgcctt ccttgatgcg
     1801 tgcgatgatg tgtggcttgc cggtgcgaag gcgcgactcg atctggttcg cagaaagtct
     1861 gtcgtggcgg atgcagacga cctttgtcgg gaaatttccg gtgggcagcg cgccgccgcc
     1921 gctctgggac cggtcgtcct ggatggttac ggtgagaccg gcggatgctt gatgcctgat
     1981 gccggaagcc agaagttccg ctttccgctc gatctcggag agcggctggg tcagcatcca
     2041 gagcgtgggg atatccctta ccgccttgtc catgtcgagg tactggccga gcgtcgcgtc
     2101 gagcgcggcc agggtcatct tgtccagacg aagcgcgcgg gtgaggggat tggaccggat
     2161 ggggtcgagg tacttttttc tgccgaggac catgcccgcc tggggacctc ccaggagctt
     2221 gtccccgctg aaggtgagca catccacgcc cgaatcaacc gcctgccgca cagtcggttc
     2281 ttcgcccgca ccgtacgacc ggaggtcgat gaaactgccg ctccccaggt cccacatgac
     2341 ggggatgttg tgctttttcc cgagggagac cagttcctca gaggatacct ccttggtgaa
     2401 cccgacgatc ttgtagttgc tcgtgtggac cttgaggagg agggccgtgt tttcgttgat
     2461 ggccttttca taatctttga gatgggtctt gttcgtggcg ccgacctcgc gcaggaccgc
     2521 tccgctgcgc tccatgacct cgggcacgcg gaaggagccg ccgatctcga caagttcgcc
     2581 ccgcgaaacg ataacctccc tgccccgggc aagggtgttg aggcagagga gcacggccgc
     2641 ggcgttgttg tttacggcgg tggcagactc tacgccggtg agccttttga gcatgttctc
     2701 gacatggaca tggcgcttgc cacgttcgcc cgccgcgatg tcgtattcga ggttggaata
     2761 acttcggctc acctccatga cccggcggat ggccgcttcc gaaaggatgg accgtccgag
     2821 attggtgtgg acgacgacac ccgttgca
//
LOCUS       DASSLD010000068         3298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1492341, whole genome shotgun sequence.
ACCESSION   DASSLD010000068 DASSLD010000000
VERSION     DASSLD010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492341"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..361)
                     /locus_tag="VFK23_01265"
     CDS             complement(77..361)
                     /locus_tag="VFK23_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7317741.1"
                     /translation="MLGTKDQIVNDLKKILVEDLFVEVPIDDIKLTDSLSTDIGLDSV
                     AHIELFAIIQDKYGINVPTDQDSLVNYRTVQSMSDFIWNNLQSAGKKVSA"
     gene            complement(520..2076)
                     /locus_tag="VFK23_01270"
     CDS             complement(520..2076)
                     /locus_tag="VFK23_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HET7317742.1"
                     /translation="MLVQSLLFDRKASSHPAVVCADRGVTASYADLARNAEELAARLA
                     RAGLRGGERAAILLPNSIEFAAAFFGSLTAGAVAVPMDIYLRKDDLLRILAVVKPAVL
                     ITNHNLFRKIKSDLGSVKACLIEYGAGLTIDFPDRNAEGTAGEDARPKSEFPDASHPD
                     RTDPAEDALMIMSSGTTGLPKAVRLSHRAVLNNIGMHLESLDLSGDIRGLQVLPLNFS
                     YGLIASFLGILRTGGTAVLLPNPEPGPIWEAIGRYDINLFSGTPTLFQYIIEKSTHVF
                     SAAPLRYLTIGGDRCKRYALDLIRSEMPSARVYITYGLSEAGPRVSTLPHELIAELPN
                     SVGLPLKGVEVSILDAEGKSCSPCTPGEIVVRSPSLMNGYFGDADRTARVLQGGRCHT
                     SDIGYVDGRGFLYYVGRKDRQFKFGGRNVNPSFIEQCISTHPLVREVHVAKIETDRDE
                     MICARIKAGDCGEEEMLRELTILCRKHMPAYMVPAEFVFEDHDHYYYKGKIFNPRKEQ
                     LPSGPVCDRR"
     gene            complement(2131..2619)
                     /locus_tag="VFK23_01275"
     CDS             complement(2131..2619)
                     /locus_tag="VFK23_01275"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HET7317743.1"
                     /translation="MAENPAREKRCIGFTEMKEKYLPHRYPIIMLDRITDYREGEYIE
                     AIKCVTGNSLELVGHFPNRAIMPATYVIQAFAQLAIVFFRLSRGQLKEDEMTLITTTK
                     FKFLAPIYPGDTINLTMKPTRLDDSIGMFSGNAVVNGKSVVRGNLTLAKTKISKIENP
                     LW"
     gene            complement(2660..>3298)
                     /locus_tag="VFK23_01280"
     CDS             complement(2660..>3298)
                     /locus_tag="VFK23_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET7317744.1"
                     /translation="FSAMRATSARNREPEKASRPFDKDRDGFVPAEGAGVLVLEGLES
                     ALRRGVPIYAEVAGYGASCDAYHITSPDPQATGAVAAIRAALHDAGIQPAEVDHVNAH
                     GTSTQLNDLVETRALKAVFGDRARKLPVSSNKSVCGHTIAAAGALEAVFSVLSIRDGV
                     VPPTLNYEVPDPELDLDYVPNRARKKELATVLSNSFGFGGANACLVFRKWNG"
BASE COUNT          621 a          970 c         1006 g          701 t
ORIGIN      
        1 cggaacgacg agattgctgc gctacgctcg caatgacggc gaaggacctg ctcgcattga
       61 ccgcttaaag agcctgttac gcggaaacct tcttgcccgc cgactggagg ttgttccaga
      121 tgaaatccga catggactgg acggtccggt agttcacgag cgagtcctgg tcagtgggga
      181 cgttgatgcc gtatttgtcc tggatgatgg cgaagagctc gatatgagcg acggaatcga
      241 ggccgatgtc ggtactcaac gagtccgtca gcttgatgtc gtcgatcggc acctcgacga
      301 acaggtcctc gacaaggatc ttcttcaaat cgttcacaat ctgatctttt gttcccagca
      361 tgttcacgtc ctccttaata gtattgcttg atcgccatta ctgtcattcc cgaaggcttt
      421 tatcgggaat ctggtttttc ttataacccc atatccgtcc ccgagtgatt ctatcgggga
      481 taaaggaatt cggggatgac aaacaggttg ttgattctcc tacctgcgat cgcacacagg
      541 ccccgaaggc agttgctcct tgcgggggtt gaatatcttg cccttgtaat agtagtggtc
      601 gtggtcctcg aaaacgaact ccgcggggac catgtaagcg ggcatatgct ttctgcagag
      661 gatggtcaac tcccggagca tttcttcctc accgcagtcc cccgccttga tcctggcgca
      721 gatcatttcg tcgcggtcgg tttcgatctt cgcaacgtgg acctcccgga ccagggggtg
      781 ggtcgagatg cactgctcga tgaaggacgg attgacgttg cggccgccga acttgaactg
      841 ccggtccttc ctgcctacgt agtacaggaa gccgcgcccg tccacatagc ctatgtcgct
      901 ggtatgacat cggccgccct gcaggaccct ggccgtccgg tcggcgtcgc cgaagtatcc
      961 gttcatgagc gacggagacc ggacaacgat ctctcccggc gtgcagggag aacagctttt
     1021 cccctcggca tcgagaatgg agacctccac ccccttgagc ggcaggccca cggagttcgg
     1081 gagctccgct atgagttcat gagggagcgt ggagaccctc gggcccgctt cgctcaggcc
     1141 gtaggtgata tagacccggg cggagggcat ctcgcttctg atgagatcga gcgcgtaacg
     1201 tttgcagcgg tcgccgccga tggtgagata tcgcagaggg gcggctgaaa agacatgggt
     1261 ggatttttcg atgatgtact ggaagagcgt gggagtcccg ctgaacagat tgatgtcgta
     1321 ccgtccgatg gcttcccaga tggggcccgg ctccgggttg ggaaggagca ccgccgtacc
     1381 gcctgtgcga agaatgccca ggaaggacgc gatcagaccg tagctgaaat tcaggggcag
     1441 gacctggagc ccgcggatgt ccccggagag gtccaggctt tcgagatgca tcccgatgtt
     1501 gttcagcacg gcccggtggg agagccgtac ggccttcgga aggcccgttg tacccgacga
     1561 catgatcatg agcgcgtctt ccgcagggtc ggtccggtcg ggatgggaag cgtcgggaaa
     1621 ttcgctcttc ggccgcgcgt cttctcccgc ggtcccttcc gcgttgcggt ccgggaaatc
     1681 gatcgtaaga ccggccccgt attcgatcag gcaggccttc acggacccga ggtccgattt
     1741 gatctttctg aaaagattat ggttcgtgat caggacggcc ggcttgacga cggcgaggat
     1801 tctgagcagg tcgtccttcc tcaagtagat gtccatgggg acggcgaccg cccccgccgt
     1861 gagcgaaccg aagaaagcgg cggcaaactc gatcgaattt ggaaggagaa tggcggcgcg
     1921 ctccccgccc cgcagccccg cgcgggcgag gcgggccgcg agttcctccg cgttccgcgc
     1981 caggtcggcg tacgatgccg tcaccccccg gtcggcgcag actacggccg gatgagagga
     2041 agcctttcgg tcaaacagga gagactggac aagcatatgg ttttccccct tgatgataat
     2101 ttcgatactc cgccgcctgt tcagagaccg ctaccagagc gggttctcta ttttcgaaat
     2161 cttcgttttt gccagcgtca ggttcccgcg gacgacgctc ttgccgttga ctacggcgtt
     2221 tccgctgaac atgccgatag agtcatcgag ccgggtgggc ttcatcgtca ggttgatcgt
     2281 atcacccgga tagatcggcg cgaggaactt gaatttcgtt gttgtgatca gggtcatctc
     2341 gtcttccttg agctgtcccc tcgaaagccg gaaaaacacg atcgcaagct gcgcgaaggc
     2401 ctggatcacg tacgttgccg gcatgatcgc ccggttcggg aaatgtccca cgagttcgag
     2461 ggagttaccc gtgacgcact tgatagcctc gatgtactcc ccttcccggt agtcggtgat
     2521 gcggtccagc atgatgattg gataacggtg gggaaggtac ttctccttca tttccgtaaa
     2581 accgatgcac ctcttctccc tcgcgggatt ctccgccatg ttcgccccct tattaaattc
     2641 cgaattccgc actccacatt taaccgttcc acttcctgaa aacaagacag gcgttcgccc
     2701 ccccgaaccc gaaggaattg gagagcacgg tggcaagctc ctttttccgc gcgcggttcg
     2761 gcacgtagtc caggtccagc tcggggtcgg gcacctcgta gttgagcgtc ggaggaacaa
     2821 cgccgtcccg gatcgaaagg acggaaaaaa cagcctccag cgctccggca gcggcgatcg
     2881 tgtggccgca caccgacttg ttggagctga ccggcagttt gcgggcacga tctccgaaaa
     2941 cggccttgag cgccctggtc tcaacgaggt cgttgagctg cgtcgaagtt ccgtgggcgt
     3001 tgacgtggtc cacctcggcg ggctggatcc ccgcgtcatg gagcgctgcc cgtatcgccg
     3061 ccacggcgcc tgttgcctga gggtcgggac tcgtgatatg gtatgcgtcg caggaggcgc
     3121 cgtagcccgc cacctcggcg tagatcggta cgccccggcg cagagccgac tccagcccct
     3181 cgagcacgag cacccccgcg ccctcggcgg gcacgaagcc gtcgcggtcc ttgtcaaagg
     3241 ggcgcgaggc cttttccggt tctctgtttc tcgcggacgt cgcccgcatt gcgctgaa
//
LOCUS       DASSLD010000069         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_122296,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000069 DASSLD010000000
VERSION     DASSLD010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122296"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..773)
                     /gene="cybH"
                     /locus_tag="VFK23_01285"
     CDS             complement(108..773)
                     /gene="cybH"
                     /locus_tag="VFK23_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HET7317745.1"
                     /translation="MPVSRIRTYAWEFPVRFTHWINVLCLIAFCITGFYIGRPFMHAI
                     SEKQYIMGWMRFIHFMSGYVFVLSMIIRIYWMFAGNRYASWRVFLPLTNRQREDFMGG
                     LRFYLFLSKKPPYAVGHSAVAGVTYFVVFILFIFEIVSGFALYSQSHLHSFSLTLLGG
                     WLLGIMHVQTIRLWHHLVMWFLIAFTLIHVYIAWYLDIAEKNGLMGSIFGGWKFMTGK
                     EWE"
     gene            complement(787..2362)
                     /locus_tag="VFK23_01290"
                     /pseudo
     CDS             complement(787..2362)
                     /locus_tag="VFK23_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512555.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
BASE COUNT          505 a          679 c          655 g          523 t
ORIGIN      
        1 tttccgaggc ccaagacaac gacacgcccg gcgtcgttgt cgtctgatac caccgggcgt
       61 gtcgcgttgc tctgaggtga gataccttcc ctgttcaaga tcaatcgcta ttcccattcc
      121 tttccggtca tgaacttcca accgccgaaa atagagccca tcaagccgtt cttttccgcg
      181 atatcgagat accacgcgat gtagacgtgg atcagtgtga atgctatcag gaaccacatg
      241 accaggtggt gccagaggcg gatggtctgc acgtgcatga tgccaaggag ccatccgccg
      301 agaagagtga gcgagaaact gtggagatgg ctctgggaat agagggcaaa ccccgagacg
      361 atctcaaaga taaacaggat gaacactaca aagtacgtga ctcccgctac tgcagagtgc
      421 cccaccgcat acggcggctt cttcgacagg aaaaggtaga atctcagtcc acccataaaa
      481 tcctcacgct gcctgttcgt aagcgggaga aagaccctcc agctcgcgta ccggttcccg
      541 gcgaacatcc agtagatccg gatgatcata ctcagcacaa acacgtatcc ggacataaaa
      601 tggataaacc gcatccaccc catgatgtac tgcttctctg atatcgcgtg catgaacgga
      661 cggccgatgt agaagccggt tatgcagaaa gcgatgagac agagaacatt tatccagtgg
      721 gtgaaccgta ccggaaactc ccatgcatac gtccgtatcc tgcttacagg catggcccac
      781 ctccttttaa acgatcctga tctttgtgac ttctttacct tccggatcca cgacgtgcac
      841 agcgcaggcc atgcaggggt cgaaggaatg gatcgtccga aggatctcta tcggcttctc
      901 cgcgtttgcg accggtgtgc cgataagcga cgcctcgtac ggtccccgct gccccttggc
      961 gtctcgcggc gaaccgttcc acgtacctgg cacgacgcat tgataattcg cgatcttttt
     1021 gtccttgatg tggatccaat ggccgaggca gccgcgaggg gcctcgtgga acccgaagcc
     1081 cttggcctct ttgggccagg tagcgggatc ccatttctcg ccgttgtgaa tgacgaggtc
     1141 tccttttttc atgttggccg cgagctgatc gagccaaacg ggaagcattt ccgccgtaac
     1201 cagggtctcg atgccgcggg ccgcgacacg gccgagcgtc gagaagagcg ccgcaggacc
     1261 gacccccagt ttcttaaggc agaaatcaac gacctctttt acgcgtttgt ggcccgaggc
     1321 ataggctaca agcatgcgcg agagcggtcc gacctccatt gctgtgtcct cgtagcgggg
     1381 ggccttgagc caggtgtact tctgtttgtc gctgacaaaa gcattttcgt aaggcggttt
     1441 ggggccgctg tacttgaagt tggtctcgcc ttcccagggg tgttttcctt tggcgtcccc
     1501 gtccttatac tcataccagg aatgggccac atattccgcg atcttggcct ggtccacggg
     1561 gagcaccttg gagaggtcct tgttcctgat gattccctgc ggcagccaca agttcttcgt
     1621 attcggcgtg ttgtcgttcg ggaagtctcc gcaggccagg aaattaccat ctccgccccc
     1681 gtatgacgcc cagtccttgt agaacgacgc tacggcgagc aggtcgggaa tgtacacctt
     1741 ttcgacgaaa tcgtgcgctt ttttcgcaag gccggaaatg aatgcaatgc tgcccgcgtt
     1801 cagggcattc tggctgttcg ggtcgacggg aatggacatg ccgcccacga ggtaggtctg
     1861 cgcatgggga ttcttggcgc ccagaagcgc ctgaatcttg atgacatcac gctgccagtc
     1921 gagtgcttcg agataatggg ccaccgccat gaggttcgcc tccggcggaa gtttgtacgc
     1981 cggatggccc cagtatccgt tggcgaaaat gccgagctgg ccgctctcca ccagggcctt
     2041 gactttttgc tgcaccccct tgaaataggt cgtggacgac ttgggccagt ccgagatcga
     2101 catggccaaa gccgaggtcc tcgccggatc cgccttcaag gcgctcacca catcgaccca
     2161 gtccagcgca tggagatggt agaaatgaat cacgtggtcc tggacatact ggatccccaa
     2221 aatcaggttt ctgatgatgc gtgcattgtc gggaattacg attcctaaag cgttttctac
     2281 agctcgaacc gagctggtgg cgtgaaccgt cgttcagacc cctcaaatac gctgggtgaa
     2341 cagccacgct tccctggggt cg
//
LOCUS       DASSLD010000070         2351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1524878, whole genome shotgun sequence.
ACCESSION   DASSLD010000070 DASSLD010000000
VERSION     DASSLD010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524878"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..1626)
                     /locus_tag="VFK23_01295"
     CDS             complement(256..1626)
                     /locus_tag="VFK23_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET7317746.1"
                     /translation="MNTSVNTSTLLADELTQLNVQIERTRQTLESLESELRAVDAELE
                     TFSEDRKRFDVLRNVCTALDKLRELKADDLFWEGVAEPQNIAGHLERAWSRIASFEGE
                     ISGILEKQASLQGQINQCLDDLDTLDQEVRDAYARDERRLEEYIVEREISPVPNRLTL
                     RKETEERFRRALIYAFLFFFCFGTVMRLWKLPPPPRPAVAVVPQRLVSMLKKQPPKPA
                     PPKPKEEKKPAKEQEKKPEKQLEQKLAHVEKVEARAKAESTGVLAFKNAFKDLMEETP
                     SARLGAEARVNNQSTVAAGQARASRSIIAMEGGSGGSGGISNAAVSRNIGRGNGIGGV
                     GFTRVESSIAGLKGESRPLSSGPGPARTDEEIQIVFDRYKATLCRMYNAELRKNPTLR
                     GKVILRLTIEPGGEVSACSVHSNDLASPELVAQIVERVKKFNFGPKADVPKTTILYPI
                     DFLPAG"
     gene            complement(1640..2110)
                     /locus_tag="VFK23_01300"
     CDS             complement(1640..2110)
                     /locus_tag="VFK23_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET7317747.1"
                     /translation="MERTNKSSGGHGFGINLIPMMDVLCVLVFFLLYHSFNSTLPEAQ
                     LTLPASVAEAHPRETVTIVVTSEAVLVQNEPVINTAKLFDDGIGLIYEVRERLVELRN
                     AVEATAKTADVSGEVTLLADKSIPFKVLKKIMSTCTASGYGKISLAVMQKEAHK"
     gene            complement(2134..>2351)
                     /locus_tag="VFK23_01305"
     CDS             complement(2134..>2351)
                     /locus_tag="VFK23_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET7317748.1"
                     /translation="YDIEMMQKMLVRLKSQYPDKEDATILMEPRIEYDYLIQVMDAVR
                     EADVKAEGSTTVEKISLFPKIALGDAP"
BASE COUNT          451 a          715 c          594 g          591 t
ORIGIN      
        1 ttcgcttctt ccaccttctg ctcggattga gccgtcgctg ctatggcggg tttctcttcc
       61 cggggtgttt caggtacagc cgacatctcg gactttttcg ggcaccccca gagggccagc
      121 atcccgatac ataatacagc catgaaatac cacgccttct tcccgttcat cccggtcata
      181 gcgaatcctc ctatgatttt cgatgcatac aatgcatctt atttaatgat ctttattcac
      241 ctccggcttc aggcattagc ctgcaggcaa gaaatcaatc gggtacagaa tggtggtctt
      301 ggggacatcc gccttcggcc cgaagttgaa cttcttaaca cgctccacaa tctgggccac
      361 cagctccggc gaggccaaat cgttcgagtg caccgaacat gcggagactt cgccgcccgg
      421 ctcgatggta aggcggagga tcaccttgcc gcgcagggtc gggttcttgc gcagctctgc
      481 gttgtacatc cgacagagcg tcgccttgta ccggtcgaag acgatctgga tctcttcgtc
      541 ggttcgtgcc ggtccgggtc cggagctcag cggcctcgac tctcctttca gccccgcaat
      601 agaactttct actctggtaa atcccacacc gccaatgcca tttccacgac cgatatttcg
      661 gctgaccgcg gcattgctga tgccaccact gcccccggag cctccctcca tggctattat
      721 tgacctgctg gcccgggcct gacctgccgc cacggtcgac tgattgttca cccgcgcttc
      781 agcgccgagt cgtgcagaag gagtctcttc catcaaatcc ttaaaggcat ttttgaaggc
      841 aaggaccccc gtgctctccg cctttgcgcg cgcttccact ttttccacgt gggccagctt
      901 ctgctccagc tgtttctccg gtttcttttc ctgctctttc gcgggtttct tttcttcttt
      961 gggcttcggc ggcgccggct tcggcggctg ctttttcagc atggagacca ggcgttgcgg
     1021 taccacggcc acggccgggc ggggcggagg cggcagcttc cacagacgca ttacggtgcc
     1081 gaaacagaaa aagaacagga aagcgtaaat caaagcccgg cggaagcgtt cttcggtctc
     1141 cttacggaga gtcaggcgat tgggaacggg tgaaatctcg cgctcaacga tgtactcttc
     1201 caggcgccgt tcgtcgcggg cgtaggcgtc gcgcacttcc tgatccaagg tgtccagatc
     1261 atcgagacat tggttgatct gcccctgaag agacgcctgt ttttccagaa tcccgctgat
     1321 ctccccttcg aagctggcaa tccggctcca cgcccgctcc agatgcccgg cgatgttttg
     1381 gggctccgca accccttccc agaaaaggtc gtccgctttg agctccctca gcttgtcgag
     1441 cgcggtgcaa acgttccgca agacatcgaa ccgctttctg tcttcggaaa aagtttccag
     1501 ctccgcatct acggcgcgta actcgctctc cagcgactcc agtgtctgac gggttcgctc
     1561 gatctgtacg ttcagctgcg tcaattcgtc cgctaacaac gtggatgtgt tcacggatgt
     1621 attcacgttg gtacccccac tatttatgcg cttccttctg catgacggca agggaaatct
     1681 ttccgtaccc ggaagccgtg caggtcgaca ttattttttt aagaaccttg aagggaatcg
     1741 atttgtcggc aaggagcgtg acttccccgc tgacatccgc cgtcttggcg gtcgcctcga
     1801 cagcattgcg aagctcaacc agccgctctc tcacttcata aatcagtcca atgccgtcat
     1861 caaacagttt ggcggtgttg atcacgggtt cgttctgaac cagtactgct tcagaggtga
     1921 ccacaatcgt cactgtttcc ctcggatgag cctcggcaac agaagctggc agagtgagct
     1981 gcgcttccgg caaggtgctg ttgaatgaat gataaagcag gaagaacacc agaacgcaaa
     2041 gcacgtccat catcggaatg aggtttatcc cgaaaccatg gccgccgctg ctcttgttgg
     2101 tgcgctccat ccgtctcatg cgtctactat tactcatggc gcgtctccta gggctatctt
     2161 cggaaagaga ctgattttct cgaccgttgt gctcccttcc gccttcacgt ctgcttcacg
     2221 aaccgcatcc atgacctgaa tcagatagtc gtattcaatc ctcggttcca tcaggatcgt
     2281 cgcgtcttcc ttgtctggat attgagactt caggcggaca agcatttttt gcatcatctc
     2341 gatatcgtat t
//
LOCUS       DASSLD010000071         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_155287,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000071 DASSLD010000000
VERSION     DASSLD010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155287"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /gene="cbiQ"
                     /locus_tag="VFK23_01310"
     CDS             <1..780
                     /gene="cbiQ"
                     /locus_tag="VFK23_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HET7317749.1"
                     /translation="LLQKLDIRFQIAGFAAILVLISLLHTPQALWLVYGAGLAIAALS
                     RVPLWFFVKRVWLFVPLFSAAVVIPAVLNVVTPGDPLWVLVKLPRSYSCGPYSLPAEI
                     AVTRQGLWGGIVFLSRVAASVSLAVLFTLTHRWADIFTGLRALHVPRVFVMTLSMTER
                     YLFVFLRLIQDMYRARKSRTIRTFSAAAERSWTASRIGVTFRKSVEMSNDVYKAMLSR
                     GFHGEFRSVDRLKSGPLDLLWLLAVIAGGGALLLFERGIIG"
     gene            777..1874
                     /locus_tag="VFK23_01315"
     CDS             777..1874
                     /locus_tag="VFK23_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7317750.1"
                     /translation="MTAVFSLHNVIYAYANGEAVLRDVSLDFRAGERTVVLGANGTGK
                     STLLSLLDGLVFPTSGSVSAFGRTLSEEAFEDRSFSMDFRKRVAFVFSNPDVQLFSAT
                     VWDDIAFGPLQLGLPPAEISGIVNGLLESLNIGELGERAPHTLSDGQKKKVAIASSLA
                     TDPDVLLLDEPTSGLDPRTQVWLIEVLHELHQKGKTIISATHDLSIAGDIAERAIVLS
                     EDHTIAADGEFYDIITNDDLLLKVNLIHEHAHSHGDTAHVHRHGHFGHIHADGGTMHD
                     THKHHVHGHEHDHSPAVEDLRKLLAMLDHWIEHGDSHVENYQEWAGKASAAGEEEVAK
                     EIHLAVDDSEAVKNHLKRAKAILAAKLVLRK"
     gene            2041..2631
                     /locus_tag="VFK23_01320"
     CDS             2041..2631
                     /locus_tag="VFK23_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HET7317751.1"
                     /translation="MQNISSEDLRDVVQFHGHLCPGLALGYRVAKAALRELGADRPQD
                     EELVAVVENDSCAADAIQFITGCTFGKGNFIFRDFGKHVYTVYNRRTGRGVRISEDFR
                     GFEGDKRFSELRARQESGEDVSREMQAYKMEKTAAILAADEKDIMTITEVTTAPPHEA
                     RLRGSMRCAVCGEKFMESRGRVKGGRIVCIPCFEKE"
BASE COUNT          599 a          846 c          870 g          597 t
ORIGIN      
        1 cttttgcaga agctcgacat caggttccag attgcgggct tcgccgccat cctggtgctc
       61 atcagcctcc tgcacacgcc gcaggccctc tggctggtct acggcgccgg cctggcgatc
      121 gccgcgttgt cgcgggtgcc gttgtggttc ttcgtcaagc gcgtctggct gttcgtgccg
      181 ctcttctccg cggccgtcgt cattcccgcg gtgctgaacg tcgtcactcc cggcgatccg
      241 ctctgggtgc tcgtgaaact cccccgttcc tattcctgcg gcccctattc tctgcccgcg
      301 gagatcgcgg tcacccgtca ggggctctgg ggcgggatcg tattcctgtc ccgtgttgcc
      361 gcatcggtct ctcttgccgt gctgtttacg ctgacgcatc gatgggcgga tatcttcacc
      421 ggcctccgcg cgctgcatgt gccacgggtt ttcgtcatga ccctcagcat gaccgagcgc
      481 tacctgttcg tttttctccg cctgatccag gatatgtaca gggccaggaa gagcaggacc
      541 atcaggacct tttccgcagc tgcggagcgg agctggacgg cgtcgcgcat cggcgtcacg
      601 ttcagaaagt cggtggagat gagcaacgac gtgtacaagg ccatgctctc gcgcggtttc
      661 cacggagagt tccggtccgt cgaccggctc aaaagcggtc ccctcgatct gctttggctt
      721 ttggccgtga ttgccggggg cggcgctctc ctcctgttcg aaaggggaat tatcgggtga
      781 ccgcggtctt cagtctccac aatgtcatct atgcctacgc gaacggggag gcggtcctca
      841 gggacgtgtc tctcgatttt cgagccggcg aacggacggt ggtcctcggg gcgaacggta
      901 cgggcaaatc cacccttctg tctctgctcg acggcctggt cttcccgacg tcggggtccg
      961 tcagcgcgtt cggcaggacg ctgtcggaag aggcgttcga ggacaggtcc ttctccatgg
     1021 atttccggaa acgcgtggcc tttgtttttt ccaatccgga tgtacagctt ttttcggcca
     1081 cggtctggga cgacatcgcc ttcggtccgc tgcagctcgg tctgccacct gcggaaatca
     1141 gcggcatcgt gaacggcctg ctcgagtcgc tcaatatcgg ggaactcgga gaacgcgccc
     1201 cgcacacact gtccgacgga cagaaaaaga aggtggccat cgcatcgagc ctggcgacgg
     1261 accccgacgt gcttctcctc gacgagccga cgagcggcct tgatccgcgg acccaggtct
     1321 ggctcatcga agttcttcac gaactccatc agaagggcaa gacgatcatt tccgcaaccc
     1381 acgacctgtc gatcgccggc gatatcgccg aacgcgcaat cgtgctctcg gaggaccaca
     1441 ccatagcggc ggacggggag ttttatgaca ttatcacgaa cgatgacctg ctgctcaagg
     1501 tgaacctgat ccacgaacac gcccactccc acggagatac ggcccacgtg caccggcacg
     1561 gtcacttcgg tcacattcac gcagacggag gaacgatgca cgacactcac aaacaccacg
     1621 tccacgggca cgaacacgat cacagccccg cggtcgaaga cctcaggaaa cttctggcca
     1681 tgctcgacca ctggatcgag catggagaca gccatgtgga aaattaccag gaatgggccg
     1741 ggaaggcaag cgccgccggc gaagaagagg ttgcgaagga gatccacctg gccgtggacg
     1801 acagcgaggc ggtgaaaaat catttgaaga gggcgaaggc aatcctggcg gcgaagctgg
     1861 tgctgaggaa ataacgagag acgagggacg agggacgaag gacgcaaagg ctttttcgtc
     1921 cgtcgtcctc gtgaacgcag cgaacggtcg tctatcgtcc ttcgtgcagt ttcgtccgtc
     1981 gtcccagcga gcagagcgaa cgatcgtcca tcgaagttga ttactaatac ggagttggtt
     2041 atgcagaaca tatcatcaga agacctgaga gacgttgtac agtttcacgg ccacctctgt
     2101 cccggcctcg ccctcggcta ccgggtcgcg aaggctgcgc tccgggagct cggtgccgac
     2161 cggccccagg acgaggaact cgtggccgtg gtagagaacg attcctgtgc tgcggacgcg
     2221 atccagttca tcaccggctg caccttcggc aaggggaatt tcattttccg ggacttcggg
     2281 aagcacgtat acactgtgta caaccgacgt accggcaggg gcgttcggat ttccgaggac
     2341 tttcgcggat tcgaagggga caagcggttt tccgagctca gggcacggca ggaatccggg
     2401 gaggacgttt cccgggagat gcaggcgtac aagatggaaa aaaccgcggc gattctcgcg
     2461 gctgatgaaa aggatatcat gaccattacc gaagtcacga cggcgccccc tcatgaagcg
     2521 aggcttcggg gctcgatgcg gtgtgcagtc tgcggcgaaa agttcatgga atcacgcggc
     2581 agggtgaagg gcggcaggat cgtgtgcatc ccgtgctttg aaaaagaata aaaatttctt
     2641 gacaccggac tgaagttcgc ctatattcca atcatgttct tccgtaaaaa tctcctcacc
     2701 cgtaagcacg tcgttatggc agcatggcac gttagtgccg tggctgaggg gggattctat
     2761 tagcttttta caaccaatag aataagtaaa aacacccaca aggccacgga aaatacaaat
     2821 tccgtggctt ttttatttat cattttggga gaaaccgcaa tgcggaaggc gaacaaggag
     2881 atcaaggacc agtcaatcat tgagggtctg ct
//
LOCUS       DASSLD010000072         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_546289,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000072 DASSLD010000000
VERSION     DASSLD010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546289"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(300..854)
                     /locus_tag="VFK23_01325"
     CDS             complement(300..854)
                     /locus_tag="VFK23_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317752.1"
                     /translation="MLQQLEEGRLYSVLDLGPARARNLDFWAPTARRICFEDYYGTWA
                     DKGFVKPEEGGSYLTLLAQLLSFREDARFDIVLAWDIFNYLDHDHVCALVEYIGRSCP
                     PGAKLLTLVSSAAIIPARPTVYQIRDREHLSYENSFPIMKPSPRYQPRDMARMIPGFQ
                     VMHSFLLRHGIQEYLFLREGANPA"
     gene            complement(985..1362)
                     /locus_tag="VFK23_01330"
     CDS             complement(985..1362)
                     /locus_tag="VFK23_01330"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET7317753.1"
                     /translation="MIGSTILIRGDVSGDEDLLIEGRVEGKIELTKHNVTIGRNGRVK
                     ADVYGKVITIEGDVQGNLFGEDRLVLRQSCTVRGNIVAPRVILEDGADFKGSIDMSPK
                     APGQEEATPSGPPSSPDPLTEDK"
BASE COUNT          426 a          663 c          673 g          467 t
ORIGIN      
        1 agacgagtgc cgacgcttat tgtcatgaag gtaatgagat tctatccgtg aggggggctt
       61 ctgacctgct ccccgaaaac ggatccccgg agggtgtgag ttctccatag acttagggaa
      121 ggagagctcg atgaagggaa aacagtttac cgaggagcag atcatcgggg tgctcaggga
      181 gccggaggta tgtgcgcgtc ctggacaaca ttccccatct cacccgctct agctgctcag
      241 tgccgccgtg ctgccggtct tcctcgagga cgcgatcttc atcggcttcc tgtagcgctc
      301 taagccgggt tcgcaccttc acggaggaaa aggtattcct gaatgccgtg acgaaggaga
      361 aaagaatgca tgacttggaa tcctgggatc atccgggcca tgtcccgcgg ttggtaacgc
      421 gggcttggct tcattatggg gaagctattc tcataactga ggtgctcgcg atcgcggatt
      481 tggtacaccg tgggtcgcgc tggaatgatc gcggccgatg agaccagggt cagcaacttg
      541 gcgccgggcg ggcacgatcg accgatgtat tccaccaggg cacagacatg atcatggtcg
      601 aggtaattga aaatgtccca ggcgagcacg atgtcaaacc gggcgtcctc gcggaacgaa
      661 agcaactgag cgagcagggt gagatacgaa ccgccctcct cgggcttcac aaatcccttg
      721 tccgcccacg tgccgtagta gtcctcaaag cagatgcggc gggcggtcgg agcccagaaa
      781 tccaagttgc gggcccgggc cgggccgagg tccagaaccg aatagagacg gccctcttca
      841 agctgctgca gcaacgcccc aagcccgagg gaccggaaaa agggggccgg aaaaccggcc
      901 gtcccgtgat cgggctctgt tgggtaggct tgagcatgtc gcattcgccg gaggacctga
      961 gatcgcaccc gacatttccc gcgactattt atcttctgtc aagggatcgg gactcgaagg
     1021 agggccggac ggcgtagctt cctcttggcc gggcgccttg gggctcatgt cgatgcttcc
     1081 cttgaagtcg gccccgtctt cgaggatcac gcgcggcgcg acgatattgc cccgaacggt
     1141 gcaggactgg cgcagcacca gccggtcctc tccgaacaag ttcccttgta cgtcaccctc
     1201 gatggtgatc acctttccgt agacgtcggc cttgacgcgg ccgttccggc ctatcgtcac
     1261 gttatgcttc gtcaattcga tcttaccctc tacacggcct tcgatcagta ggtcttcatc
     1321 gccgcttaca tcacccctta tcaatatggt cgatccgatc aaggcacact cctttggtga
     1381 tgacacgggg gccacacttc gcggtggcgg tgccggcgcc ggctgctcgg ggagtgggac
     1441 tctggactcc ctgttctctt gctgtttcca cactacggaa tcctcccgga atagggattg
     1501 acacgggacg gctaattctc acgagatccc ctgtgcctcg tcaaactctc atccaggcgt
     1561 ccgggccgta ggtccttcgt tttcttgaac ttctgtaggg gtgtgtaggg ctggcagtcg
     1621 gactgctcgc acacacctcg agcgagtctg aagcgggcag ggaccgacgc ccccggcgcc
     1681 ggcggcgccg ctcttcggag gagtacaggc aggcggcgct cgggatcgag gtaaacgggc
     1741 cggaacagcg gctcagccag tccctgccgc agaggctggt gcagcctagt gactggggtg
     1801 gcactcttca gcgtagccgg acgcgtccac gtaaccgctt cctttcgaag ggaatccagt
     1861 ctgagaacat tttgaagttc tcggattctg acttgagaac agttcgtcta gccgaccgag
     1921 tccggagcca gacctctccc acgcgaactt cagatcggtt cgcggttatc acagaccccg
     1981 cgattcgcgt cgcggggttt cgcgtctaaa ggtgaaccgc gtctccttca ccgagctctc
     2041 tttccctaag tctctctcac cctccgtggc tccgttttcg gggagcaggt cacgtttacg
     2101 aaactggatt caaagggccc cggcaattcc cgctgcgccc tacatcccca ctgacgcttt
     2161 tgttttctcg aagacctctt ctgacgcgac ttctccggag gaggaagtgg cgggtcgtgc
     2221 gggacggct
//
LOCUS       DASSLD010000073         2907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_253350,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000073 DASSLD010000000
VERSION     DASSLD010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253350"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VFK23_01335"
     CDS             complement(<1..427)
                     /locus_tag="VFK23_01335"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HET7317754.1"
                     /translation="METQKGIVEIVADKVWKFFSSVKLAVVLLIILAIVSVIGTVIQQ
                     NQAPEQYLREYSQSTVQFFEMLGFFDMYHTWWFVLLLFLFTANLTVCTLDRLPHTLKI
                     MTAPLKPIDDNSLRAVPYKKEIAFNGDMIKAEERSLGVLK"
     gene            complement(585..806)
                     /locus_tag="VFK23_01340"
     CDS             complement(585..806)
                     /locus_tag="VFK23_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HET7317755.1"
                     /translation="MGKVSLSVQKQFCAECSLALRRFIGNLDGIEAVDVGNGTIDISF
                     DGSKISEEMVRKLSRDSVEKLGYKLLNDE"
     gene            complement(820..1722)
                     /locus_tag="VFK23_01345"
     CDS             complement(820..1722)
                     /locus_tag="VFK23_01345"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HET7317756.1"
                     /translation="MIKRKIFNNLFVIVSILATIFVATEIVLKFFGRSICMTEGCKMT
                     AQYARFGDISILLIGLAAFSSIASLSLLNRHAQRAGIERLVNLILIVALTGEGFFMGY
                     LAFKIHTLCVFCVIVFCIMMTLGTLRVLSGEREAAAGFATLAAVFLMHYLILPAGVTA
                     KLPVHDRLVLFYSKDCRHCAEIIRELEEKKIAVTHLSVSEYGGYLKNLGIEYIPTLMV
                     NDPNEKVFLTGKEMIRRYLESCSETNKPGEKITQQKPGRKTAAPAPRDSNVTLDIFRQ
                     PGLLTMPSQSEPGSGICREEEVCK"
     gene            complement(1921..>2907)
                     /locus_tag="VFK23_01350"
     CDS             complement(1921..>2907)
                     /locus_tag="VFK23_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET7317757.1"
                     /translation="KHDNLPVVLVTKDTNLRIKADALGLRAEDYESETITIDELYSGE
                     TELLVEPGIIDTFYAQGEFTPPAEFKPHPNEFVLLKNSANPSQTALARHYLQKNVLVP
                     VINTKHGVWGINARNKQQQFALDLLLNDDIRLVTLVGKAGTGKTLLALAAGLEKTIEA
                     RAFQRLVVSRPVFPLGKDIGFLPGDIEEKLRPWMQPIRDNLDFLMGASAVAGRAKGKK
                     DLQSLFDLGMIEVEPLTYIRGRSMPNQYLIVDEAQNLTPHEIKTIITRAGEGTKIVLT
                     GDPYQIDNPYIDSSSNGLSFVVERFKEEAIAGHITLVKGERSDLAELAATLL"
BASE COUNT          609 a          794 c          850 g          654 t
ORIGIN      
        1 ccttgagcac gcccaatgat cgttcctcgg ctttaatcat gtcgccgttg aaggcgatct
       61 ccttcttgta cggaaccgcc ctgaggctgt tgtcgtcgat gggtttgagc ggggccgtca
      121 tgatcttgag cgtatggggc agacggtcga gggtgcagac cgtgaggttg gccgtgaaga
      181 gaaagagcag aagcacgaac caccaggtgt gatacatgtc gaagaagccg agcatctcga
      241 agaactgcac cgtggactgg ctgtattccc gcaggtattg ttccggggcc tggttctgct
      301 ggatcaccgt gccgatgacc gatacgatcg cgaggatgat gagcagcacg accgcgagct
      361 tcacggaaga gaaaaatttc cagaccttgt cagcgacgat ttcaacgatg cctttttggg
      421 tttccaacac gacctcccga tgcgaaacag tttattaagt taacacaatg actacggggt
      481 gtcaaggagt ttactatgca taccaggatg atcattcgcg gaaaatgacg gacgacccag
      541 gctgcaggag gatcggcgca ggcaggcacc cgggggactg gcctctattc gtcgttcaaa
      601 agtttatagc cgagtttctc aacgctgtcg cgagacagtt tccggaccat ctcctcgctg
      661 atcttcgaac cgtcaaagct gatgtcgatg gtcccgttcc ccacatcaac ggcctcgatg
      721 ccgtccaggt ttccgataaa tcgcctgagg gccagggaac actccgcgca gaactgcttt
      781 tgaacggaaa gagacacctt tcccattgcc gcctccttgt cacttgcaga cctcttcctc
      841 cctgcatatg cccgagcccg gctccgattg actcggcatg gtaaggagac ccggctgtct
      901 gaagatgtcg agggtcacat tcgaatcccg gggggcggga gcggccgttt tccggccggg
      961 tttctgctgc gtgatctttt ccccgggctt gttcgtttcc gaacaggact ccaggtagcg
     1021 cctgatcatc tccttgcccg tgagaaacac cttctcattg ggatcgttca ccataagggt
     1081 ggggatatat tcgatgccca agttcttcag atagccgccg tattcgctga cagaaagatg
     1141 cgtgacggcg atctttttct cttccagctc ccggatgatc tccgcgcagt gcctgcagtc
     1201 cttgctgtaa aacagaacga gccggtcatg cacgggcagt ttggccgtca ctcccgcggg
     1261 caggatgaga tagtgcatca ggaagaccgc cgcaagggtc gcgaagccgg cggcggcttc
     1321 ccgttcaccg gaaagtaccc tgagggttcc gagggtcatc atgatgcaga agacgataac
     1381 gcagaagacg cagagcgtat ggattttgaa cgccagatag cccatgaaga aaccctcgcc
     1441 ggtcagggcg acgatcaaga tgagattgac gaggcgttca atcccggccc tctgggcgtg
     1501 ccgattcagg agcgaaaggg acgcgatcga ggaaaacgca gccagtccga tcagcaatat
     1561 ggagatatcg ccgaaccgag catactgggc cgtcattttg cacccctcgg tcatgcagat
     1621 gctcctgccg aaaaacttca agacaatctc ggtcgcgacg aagatcgtcg caaggatgct
     1681 cacgattacg aacaggttat tgaatatttt gcgtttaatc ataattcata attacacggg
     1741 ctgtcattcc cgcccctgct ttagtcatgc tggggcaggc tccagcggga atccattggc
     1801 tgttcaagcc aattcaaaat ggattcgtcc cggaatgaat ttatcgggga tagaatcatt
     1861 cgggaatgac gattcggtct tatctagtct tgttcatgca caccggactc tgtaccgtgt
     1921 ttataacaac gtcgcggcga gttcggcgag gtcggaccgt tcgcccttga cgagcgtgat
     1981 gtgcccggcg atcgcctcct ccttgaaccg ctcgacgacg aagctgaggc cgttgctcga
     2041 agaatcgata tagggattgt ctatctggta cggatcgccc gtcaatacga tcttggtgcc
     2101 ttcgcccgcg cgcgtgatga tggtcttgat ctcgtgcggc gtgaggttct gcgcctcgtc
     2161 cacaataagg tactggttcg gcatactcct gccgcggata taggtgagcg gctcgacctc
     2221 gatcatgccg aggtcgaaga ggctctgaag gtctttcttg cccttggccc tgccggccac
     2281 ggccgacgcg cccatgagaa aatcaagatt gtcgcggatg ggctgcatcc agggccggag
     2341 tttctcttcg atgtcgccgg gcaaaaagcc gatgtccttg ccgaggggaa acacggggcg
     2401 ggaaacgacc aggcgctgga atgcccgggc ctcgatggtt ttctccaggc cggccgcgag
     2461 cgccaggagg gtcttgcccg ttccggcctt gcccacgagc gtcaccagac ggatgtcgtc
     2521 gttgaggagc aggtcgaggg caaactgctg ctgtttgttc ctcgcgttga tgccccatac
     2581 gccgtgcttt gtatttatga ccggcaccag gacgttcttc tgcagatagt gacgtgcgag
     2641 ggccgtctgg gacggattgg cgctgttctt gagcagcacg aactcgttcg gatggggttt
     2701 aaactcggca ggcggcgtga attcaccctg cgcgtaaaag gtgtcgatga tgcccggctc
     2761 gacaagaagc tccgtctctc cggaatacag ttcatcgatg gtgatcgtct ccgactcata
     2821 atcctcggcc cggagcccga gggcatcggc tttgatacgg agattcgtgt ctttcgtcac
     2881 gaggaccacc gggagattgt cgtgctt
//
LOCUS       DASSLD010000074         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_415877,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000074 DASSLD010000000
VERSION     DASSLD010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415877"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..328)
                     /locus_tag="VFK23_01355"
     CDS             complement(83..328)
                     /locus_tag="VFK23_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317758.1"
                     /translation="MYQIWIAVIGAIIMAGGLGGVLYLIIKQQAAINTRLIQFLTIAF
                     VLPLTLVLGIMNTLGRETIGTLLGVVVGYVLSGFAKE"
     gene            complement(607..873)
                     /locus_tag="VFK23_01360"
     CDS             complement(607..873)
                     /locus_tag="VFK23_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317759.1"
                     /translation="MRYSTPRPPVSPSIFSSNLPSPREKLGEREPHDGSSVPRLPEVP
                     GELCGGFVKRVEMFVLEQKGDLDKAVVLMPLMAAAVMTVFKMFV"
     gene            930..1802
                     /locus_tag="VFK23_01365"
     CDS             930..1802
                     /locus_tag="VFK23_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7317760.1"
                     /translation="MAYATGSKPYLVDIHVHGADKYDTRTRRQDDILQVAGIHGKRGT
                     SALLPTVYPGDIGLMRENMAAIRRAMSAQRDEAAILGIHLEGPFLNPARSGSLDGKSF
                     SDPAVETFTRLVEDFEDIIRIITIAPELPGALRLIELSREKGFLVHMGHSDASFEQAE
                     EGKRAGATGITHLFNAMRGFHHREPGLAGFGIMDEDIYVEIIADLAHLHPQSLKMIFD
                     MKSLDKIILVSDSVMGPGWGSGPIRGAGGTLLGSGITLADAVKNLIALGVPQERALRF
                     ASVNPMKYLGINSV"
     gene            1980..2633
                     /locus_tag="VFK23_01370"
     CDS             1980..2633
                     /locus_tag="VFK23_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET7317761.1"
                     /translation="MKPIDLIIFDLDGTLVNTLEDIAASVNYTLQKLGRPLIPIDSVR
                     QYVGDGVEMLMRRALSGSDEFLPDAVGIYKVHHSRNLLVRSRLYPSVTEVLEHFRNLP
                     MAVLSNKTSEFVQPLLDGLKISGYFRVVLGAGSGLPLKPAPDAVQKIMAEFEASKDRT
                     VIVGDGTADVRAGKAAGVITCSVTYGFRSEEELRKAGPDYIIHELSELKGLFTPMIR"
BASE COUNT          626 a          851 c          833 g          609 t
ORIGIN      
        1 ggggaacatc ggcagcttct gtgcggctat ataggctgta taaatgatgc cactccgcgt
       61 ctccccgtcc ccgcgtcggt ttttattctt ttgcaaaacc cgaaagcaca tagccgacga
      121 cgacgccgag gagggtgccg atggtctccc tgccgagcgt attcatgatg ccgagaacga
      181 gcgtgagcgg caggacgaag gcgatcgtca ggaactggat cagcctcgtg ttgatcgccg
      241 cctgctgctt gatgatgaga tagaggaccc cgccgagccc tcccgccatg atgatggccc
      301 cgatgaccgc aatccagatc tgatacatgt gtccctccgg atgtttcgag atttcgcaga
      361 accatttctg cttatcgcag gccgctatta tatgacaccg cggttgtctt gtcaaaggtt
      421 ccgtggcgac gcaacactgc cgggccgcct tcggcactgg caacttttcg aaaaagcggg
      481 tacaatagcc gtattggatg ctggatacgg ggtactcgat atttgatacc ggataaaccg
      541 aaacgcatcc tttgccttcc taattcattc agaatctgag gacctatgga cgacaaacac
      601 gaacattcac acaaacatct tgaacacggt catcacggcc gcggccatga ggggcatgag
      661 caccacggcc ttgtcgaggt cacccttctg ctcgaggacg aacatctcga cgcgcttgac
      721 gaaaccgccg cagagctcac ccgggacctc gggcagacgt gggaccgagg agccgtcgtg
      781 cggctcgcgc tctccgagtt tttctcgcgg agagggaaga ttacttgaga agatagacgg
      841 ggagacaggg ggacgcggag tcgaataccg catccccgcg tctccgcgtc gccccgtccc
      901 cgtgtcgttt ttttagtcga gggttcatca tggcctatgc gacgggcagc aagccgtacc
      961 ttgtcgatat ccatgttcac ggcgcggaca aatacgatac gcgaacgcgc cgtcaggacg
     1021 acatcctcca ggttgccggc atccacggca agcgggggac atccgcgcta ttgccgacgg
     1081 tgtatccggg agacatcggg ctcatgcgcg agaacatggc ggccatcagg cgggccatgt
     1141 cggcgcagcg ggacgaggcc gcgatcctcg ggatacacct ggaaggccct tttctgaacc
     1201 ctgcgcggtc cggttctctg gacggaaaga gcttttccga tcccgccgtc gagaccttta
     1261 cgcggctcgt cgaggacttc gaagacatca tcaggatcat caccattgca cccgagcttc
     1321 cgggggcgct ccggttgatc gagctgagcc gcgaaaaagg gttcctggtt cacatgggcc
     1381 acagcgacgc gtcgttcgaa caggcggagg aaggaaagcg cgccggcgca acgggcatca
     1441 cgcacctgtt caacgccatg cgcgggtttc atcaccgcga gcccggcctt gccggtttcg
     1501 ggatcatgga cgaggatatc tacgtggaga tcatcgcgga cctggcccat ctgcaccctc
     1561 agagcctcaa gatgatcttc gacatgaaat cgctggacaa gatcatcctg gtctccgatt
     1621 cggtcatggg tccgggctgg ggcagcggcc cgatacgggg cgccgggggc accctcctgg
     1681 gcagcggaat taccctcgcc gacgcggtca agaacctgat cgcgctcggt gtgccccagg
     1741 aacgggcatt gcggttcgcc tcggtcaatc cgatgaagta cctggggatc aactcggttt
     1801 gattcaaact ccaatatcga aatcctgaat tccatgatga gggtcgcaaa aaagtcccaa
     1861 atcatctaat ttgtcattct gaggcgaagc cgaagaatct ggatattcat gccgatgcca
     1921 gattcttcac ttctcttcgc tccgttcaga atgacggcaa agagtgtgta gaatttttca
     1981 tgaaaccaat cgacctcatc atcttcgacc tcgacggcac cctcgtgaac accctggagg
     2041 acatcgccgc gtccgtcaat tatactcttc agaagctcgg caggccgctt atcccgattg
     2101 attccgtccg gcagtatgtg ggcgacggcg tcgagatgct catgaggcga gcgctcagcg
     2161 gcagcgatga gttcctgccc gacgcggtcg gcatctacaa ggtccatcac agcaggaacc
     2221 tgcttgtgcg ctcgcgactc tatccctcag tgacggaggt gctcgaacac tttcgcaacc
     2281 tcccgatggc agtgctcagc aacaagactt ccgaattcgt ccagccgctc ctggacggcc
     2341 tgaagatttc aggctacttc cgcgtggtcc tcggcgccgg ttcagggttg ccgctcaaac
     2401 ccgcaccgga cgccgtgcag aagatcatgg ctgagttcga ggcctcgaaa gaccgtaccg
     2461 tgatcgtagg tgacggcact gcggacgtgc gtgctggcaa ggcggcggga gtcatcacct
     2521 gctccgtgac gtatgggttc cggtccgagg aggaactgcg aaaggcaggg ccggattaca
     2581 tcatccacga gctttcggaa ttgaaagggc ttttcacgcc catgatacgg tgagacggca
     2641 gcgcctgccg cgtccccgga attttgcctt tactattccc gtctgattgg ttaaaataat
     2701 aagtctctgg ttcagagtta aaattggtct taatatacaa tgtcattccc gcccctgctt
     2761 gaatcatgct ggggcaggcc ctgatcggga acccattttt tgtcccagca aaatgggatg
     2821 accgatagag tcattcgggg atgatcaact atctggtaat tttataagga gtcccaatgt
     2881 cccatcccca tgcaacgaaa gcgccccacg ggcacggcc
//
LOCUS       DASSLD010000075         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_512952,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000075 DASSLD010000000
VERSION     DASSLD010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512952"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..459)
                     /locus_tag="VFK23_01375"
     CDS             complement(241..459)
                     /locus_tag="VFK23_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET7317762.1"
                     /translation="MKIDVYMSETCGSYYRLRENMEHALSESGASAEVEYHTIYYDDA
                     VDRHIKGSPSIWINGRDAFESGNAPGIT"
     gene            complement(560..883)
                     /locus_tag="VFK23_01380"
     CDS             complement(560..883)
                     /locus_tag="VFK23_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7317763.1"
                     /translation="MPKYPQQYMSIMKKYTGVIKALDGLGRATKKAGPLNKKFSELIQ
                     LAAAAAVRSEGSVHSHTRRALAAGAKPEEVRHAIVLLTSTIGFPAVSAALSWTDDVLE
                     KKRLI"
     gene            970..1872
                     /locus_tag="VFK23_01385"
     CDS             970..1872
                     /locus_tag="VFK23_01385"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET7317764.1"
                     /translation="MTRGERLCYVLPMDTASSLRRDVTVLAREIGVRSYRDRDRLQKA
                     ADYISVRLVSFGYAVSRQPFQFRGNTYDNLIAELSGSSSPEKILVVGAHYDTVRTTPG
                     ADDNASGVAGLLGLAQALADSRPKKTVRFVAFALEEPPVYRTRNMGSYHYARSLKEDR
                     DDVEGMICLEMIGYFREGKHSQHYPFPFMKRVYPRAGNYISMVGNLWSRDFTRTMAED
                     FRKAVDLPVVTLNAPPIVIGIDFSDHWSFGKFGYRAFMVTDTAFYRNPHYHAPSDTPE
                     TLDYERMAEVVSGLRAAVEKWGRT"
BASE COUNT          439 a          602 c          608 g          466 t
ORIGIN      
        1 gccagtcgaa ccactgggtc aatgaatcgc cgatgaagac aaggtttctg gacattgtaa
       61 ttgcaggggg cggagggtgg gattccgcat agtgcaaagc gcatagagca tagcgtcaaa
      121 gcacgaatca acgctctgcg ccatgcgcta tgctccgctc gttaccttcc tgaacgcctg
      181 cttcagtatt tccacggaag gcgcgttctg gagtctcccg gactcgtccc ggtacagcct
      241 tcaggtaatg ccgggagcat tgccgctttc aaaggcgtct cttccattga tccagatgga
      301 gggggaacct ttgatgtgtc tgtcgacagc gtcatcgtag tagatcgtgt gatactccac
      361 ctcggcactg gcgcctgatt ctgaaagcgc atgttccata ttctcccgga gccggtagta
      421 ggatccgcag gtctcggaca tgtagacgtc gatcttcata acgcgtcaaa aatgctggat
      481 gccagatact ggatactggg ttcaggatgc cgaatttatc caccatcgag tatccagtat
      541 ccacatcgtt tctcgtttac tagatgagtc tcttcttctc cagcacgtcg tccgtccagg
      601 agagcgcggc ggagacagcg ggaaatccga tggtgctggt caagagaacg atggcgtggc
      661 ggacctcctc gggcttcgcg ccggcagcga gcgcccggcg ggtgtgcgag tgcaccgatc
      721 cctcggaacg gacggccgcc gctgccgcga gctggatcag ttccgaaaat ttcttattca
      781 acggtccggc tttcttggtc gccctgccga ggccgtcgag cgccttgatc actcccgtgt
      841 attttttcat gatgctcata tactgctggg gatacttcgg cattggttcc tccctggtta
      901 tcgcatggat tttccacctg taaaactaag tttatgcaaa gaggaccgaa gagtcaagga
      961 gattggtgtt tgacacgcgg cgaacgacta tgctacgttt tacccatgga caccgcttcc
     1021 tctcttcgcc gggatgtgac cgtgcttgcc cgggagatcg gcgtcagaag ctatcgcgat
     1081 cgggaccgcc ttcaaaaggc cgcggactac atttccgtgc gactcgtgtc gttcgggtat
     1141 gccgtatcgc gtcagccctt tcaattccgc gggaacacct atgacaattt aatcgccgaa
     1201 ctgagcggct cgtcctcgcc ggaaaagatc ctggtcgtcg gcgcgcacta cgataccgtc
     1261 cgcaccacgc cgggggccga tgacaacgca agcggcgttg ccgggctgct cggactggcg
     1321 caggccctcg cggacagccg gcccaaaaaa acagtccggt tcgtcgcttt cgccctcgaa
     1381 gaaccgcctg tgtaccggac caggaacatg gggagctatc actatgcgcg aagcctcaag
     1441 gaggaccggg atgacgtgga aggcatgatc tgcctcgaga tgatcggcta tttccgcgag
     1501 ggcaagcaca gccagcacta tccctttccg ttcatgaaaa gggtctaccc cagggccggc
     1561 aactatatct ccatggtcgg gaacctgtgg tcgcgggatt ttacgaggac gatggctgag
     1621 gatttccgaa aggccgtgga cctgcccgtg gtcacgctca atgcaccgcc gatcgtgatc
     1681 ggcatcgatt tttccgatca ctggtcgttc gggaagttcg gatacagggc attcatggtg
     1741 acggacacgg cgttttaccg gaacccccat tatcacgcac catccgacac gccggaaacg
     1801 ctggattatg aacgtatggc cgaggtcgta tcaggactgc gggcggcggt ggagaagtgg
     1861 gggagaacgt gagttcaaag ttcagagtcc ggagttcaga gtagaagcaa ccttgtaact
     1921 tcgtatttga cattcccccc ctgccattat atgctgtcct gagagagcca tcacatgatc
     1981 ctgaacggaa tccaagaggc tgccgcacgc cgtcccgatc gcatcgcggt gcagatgaaa
     2041 acgggtgacg gctatgtccg gttcacctac cgggacctcc tgaattccat cgcctccgcg
     2101 tcccgggcgc tctcc
//
LOCUS       DASSLD010000076         4222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_482619,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000076 DASSLD010000000
VERSION     DASSLD010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482619"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /gene="hemE"
                     /locus_tag="VFK23_01390"
     CDS             complement(<1..790)
                     /gene="hemE"
                     /locus_tag="VFK23_01390"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HET7317765.1"
                     /translation="MSRADRFLKACRRESVDCTPVWMMRQAGRYLPEYREIRAKHSFL
                     EMCKTPELAAEVTIQPVRRYEIDAAIIFADILLPLEPMGVGLEFAKGEGPVIHNPVRT
                     MADVERLKPIDAARQIEYLMKAIRIVLRELDGKVPLIGFTGAPFTLASYIIEGGGSRN
                     YEHAKAMMFSEPKTWHKLMEHLTGVIINYLNAQIDTGVQAVQVFDSWVGALGPSDYRE
                     FVLPHQKNVIAGIKKTVPLIHFAHGAAHLLEMVSEAGGDVIGLDW"
     gene            986..1894
                     /locus_tag="VFK23_01395"
     CDS             986..1894
                     /locus_tag="VFK23_01395"
                     /inference="COORDINATES: protein motif:HMM:NF020476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HET7317766.1"
                     /translation="MSNVISASRRTDIPAFYADWFIRRLRAGSVTVLQPYSRRPSVVS
                     LLPEDVSAVVFWSKNYAPLLSRLDAVEKTSRNLFFHFTITANHELEGAVPDHRDAVKD
                     FLFLRRRYGTDRVLWRYDPICVTDKLSFEMHDERFAMLAEQLEGQVKKCVISFVHPYK
                     KVVANMAKYGNQTLAPLSDEVKRDFALRLALRAEVKGIQMYACCNDYLLSGSVGKASC
                     VDGAYLSKIFKTPLDTRPASLREECACTRSIDIGAYDTCGHGCLYCYANTDKNRAGAA
                     LLKHDPSWNALGGQVNDAGVLSGAKE"
     gene            1988..3175
                     /locus_tag="VFK23_01400"
     CDS             1988..3175
                     /locus_tag="VFK23_01400"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HET7317767.1"
                     /translation="MDKRKIFIIAILLSSLFACARVPAPVTRPEDALAPGRMHAALHD
                     DMEFRGIAASVRQSLEYYKKLPPDAAFQFGPDRVTGLDLVATLQAFLRIVEDDSLTSG
                     QKIDRIRNDFVFYRAAGSDRKGKMLFTGYYEPMISCRTSVDKTHRYPLYRRPDDILEI
                     DLTQFGNGFPKNKIYGRLNGRKVVPYYSREEIDRKKVLSGKGLEILWCTDPVDIFVLQ
                     VQGSGKADLGDGSVVSVLYDASNGRPYTSIGRHLIDTGAIAKENMSMQAIRQYLRDNP
                     AKLAEVLDRNQSYVFFRLDTGPSLGNIGVPLTPGRSIATDSRLFPKGALALMVSRKPV
                     IENGVIKDWTPFTRFVVNQDTGGAIKGAGRADVFFGQGPEAELSAGNFQEEAELYFLV
                     KRK"
     gene            3803..>4222
                     /locus_tag="VFK23_01405"
     CDS             3803..>4222
                     /locus_tag="VFK23_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317768.1"
                     /translation="MNSATPINSTYNGKVSGTETDTGSIDPDTGKGTLTVTYVNFNDG
                     DGRTFDGAFSVKVGAYDMAYDMMTDWTMSCTLWTVKSAGSHVSLSGSVREQKNLQGNS
                     DTTTINVDGRDNIGNETFRFENYIVTAVSDNLLNPTTG"
BASE COUNT          898 a         1225 c         1218 g          881 t
ORIGIN      
        1 gccagtccag gccgatcacg tcgccgcccg cttcggagac catctccagg agatgggccg
       61 ccccgtgggc gaaatgaatg agcggcactg ttttcttgat cccggcgatc acgttcttct
      121 ggtgcggcag cacgaactcg cggtagtcgg acggacccag ggcgccgacc caggagtcga
      181 agacctggac ggcctgcacg cccgtatcga tctgggcgtt caggtagttg atgataacgc
      241 ccgtcaggtg ctccatgagc ttgtgccagg ttttcggctc cgagaacatc atggccttgg
      301 catgctcgta gttccgcgag ccgccgccct cgatgatgta ggaggcgagc gtgaacggcg
      361 cgcccgtgaa gccgatgagc ggcaccttgc cgtcaagttc ccggaggacg atcctgatgg
      421 ccttcatgag gtactcgatc tgccgcgcag cgtcgatggg cttgagcctt tccacgtctg
      481 ccatggtgcg gacgggattg tggatcaccg gtccctcgcc cttggcgaat tccagcccca
      541 cccccatggg ctccagcggc agcaggatgt cggcgaagat gatggcggcg tcgatctcgt
      601 aacgccggac aggctggatc gtcacctcgg cggcgagttc gggcgtcttg cacatctcga
      661 ggaacgagtg tttggcccga atctcacggt actcgggcag gtatctgccc gcctggcgca
      721 tcatccacac cggcgtgcag tcgacactct cgcgcctgca tgccttcaaa aacctgtctg
      781 ctctggacat ccgtatgttc tccctgtgcc ggaaatggtg tgactcatgc tggaatgaat
      841 gcccataata gtattttgcg gctgaaaact caagaaatat ttcgggcgaa accggccgcc
      901 cttgccgggt ttcgcgcgcc cgccgtacgc tgctcttgtt ttttcgggac gattgatgta
      961 tgtatggacg aacggagcgt gccccatgtc gaacgtcatc tcggcgagca gaaggaccga
     1021 catccccgca ttttacgcgg actggttcat aaggagactt cgggccgggt cggtcaccgt
     1081 gctccagccc tactcccgaa ggccgtctgt cgtatccctt ctgccggagg acgtgagcgc
     1141 cgtcgtcttc tggtcaaaga attacgcacc gcttctctcc aggcttgacg ctgtcgagaa
     1201 gacctccagg aacctgttct tccattttac gataaccgca aaccatgagc tggaaggcgc
     1261 ggtgcccgat catcgggatg cggtcaagga ttttcttttc ttgcgccgcc gctacgggac
     1321 ggaccgggtg ctctggagat acgatcccat ctgtgtcacc gataagcttt cttttgaaat
     1381 gcatgatgag cgctttgcca tgctggcgga acagctggag ggacaggtga agaagtgcgt
     1441 gatcagcttt gtccatccct ataagaaggt cgttgccaat atggcgaaat acgggaacca
     1501 gacgctcgca cccctgtccg atgaggtcaa acgggacttc gcactccggc tggccctgcg
     1561 ggccgaagta aagggcatcc agatgtatgc gtgctgcaac gactacctgc tttcgggcag
     1621 cgtgggaaag gcaagctgcg tcgacggcgc ctatctgtcg aagatcttca agacgccgct
     1681 cgataccagg cccgcttccc tgcgcgagga gtgcgcctgc accaggagca tcgatatcgg
     1741 ggcgtacgac acctgcggcc acgggtgcct gtactgctac gcgaacacgg acaagaacag
     1801 ggccggggcc gcgctcctga agcatgatcc ttcctggaat gcgctgggcg gccaggtgaa
     1861 tgacgctggc gtcctctcgg gagctaagga gtagccccgt aatatcgttt tacaaattgg
     1921 cctgacctct gctagaatcc ggatgttcga tccatcacgt ggttcaggcc ggctatcggt
     1981 accgaaattg gataagcgta aaatattcat catcgccata cttctctcgt ccctcttcgc
     2041 ctgcgcccgc gtccccgctc ccgtgaccag gcccgaggac gcgctcgcgc ccgggcggat
     2101 gcatgcggct ttgcacgacg acatggagtt ccgcggcatc gccgcttccg tccgtcagag
     2161 cctggagtat tacaagaagc tgccgcccga tgctgcgttt caattcggac ccgaccgggt
     2221 gaccggactc gacctggtcg ccaccctgca ggcctttctc cgcatcgtgg aggatgattc
     2281 cctgacttcc gggcagaaga tcgaccgcat cagaaacgat ttcgtttttt accgcgcagc
     2341 gggttcggac cggaagggca agatgttgtt taccggatat tacgagccca tgatttcctg
     2401 ccggacgtcc gttgacaaga cccaccggta tccgctgtac cggaggcccg acgatatcct
     2461 cgagatcgac ctcacccagt ttggaaacgg gtttccgaaa aacaaaatct acggaagact
     2521 gaacgggaga aaagttgtcc cctactactc gcgggaggag atcgaccgga agaaggtcct
     2581 ttccggcaaa ggccttgaga tcctttggtg cacggacccc gtcgatatct tcgtgctcca
     2641 ggtccagggc tcgggcaaag cggacctggg agacggcagc gtcgtgagcg ttctgtacga
     2701 cgcttcgaac ggcaggccct atacgagtat cggcaggcac ctgatcgaca ccggagccat
     2761 cgccaaggag aacatgtcca tgcaggcgat ccgccaatac ctgcgggaca atccggcgaa
     2821 gctcgcggag gtcctcgacc ggaaccagag ctatgtcttc ttccggctcg acacggggcc
     2881 ctcgctcggg aacatcggcg ttccgctcac gcccggaagg tcaatcgcca ccgactccag
     2941 gctcttcccc aagggcgccc tggccctcat ggtcagcagg aagccggtca tcgagaacgg
     3001 cgtcatcaag gactggacgc ccttcacccg cttcgtcgtg aaccaggaca cgggaggcgc
     3061 aatcaagggc gccggccgcg cggacgtgtt cttcggccag ggcccggagg ccgagctttc
     3121 cgccgggaac ttccaggagg aggcggagtt gtatttccta gtgaagagga agtgaatttg
     3181 actgttccag agtgggtccg ataaccacga aaaatcaagc tgcaagtccg attagaaatg
     3241 aagacacatc ccacgctatt cgtaaagttg taatacttcc ggcctacccg ataaataccc
     3301 catttatttt ctttatcccc ctgcatgaga gcgttatatt aatgcaatct tttgtcgatc
     3361 ggaagatgct ttaaccgcat agccgcgata tatcgcgtga taagcgttcg acatgcccga
     3421 ccattggatt agcagatata tgaaaaaatt tataatgagg ttatcgagac cgcctctgcc
     3481 gacgcttctc atccttgcag gattatcagc cctcttgtca gcatgtaccg gaagcggcgg
     3541 gggcggaacc ggagcggaag cagggggcat cacgtatgcg ggggcgacta ctccggcttc
     3601 tctttccgcg tcaaacgcga atatcatatt ttcagcgatg tggaatggag gctatccggc
     3661 cggatcaact tctctctcgc ccgctatctc gaaagcggct ccatccggaa attcgcaaga
     3721 caatggaatc gcgtctctgg taaagcagac gcaagagcgc ctgtcaggta aagtcatcag
     3781 gttctccacc cggtcgaaaa acatgaatag cgcaacgccg attaacagta cttacaacgg
     3841 caaggtaagc gggacggaga cggacaccgg cagtatcgat cctgataccg gcaagggcac
     3901 tttgaccgta acttatgtca actttaatga cggtgatggc cgtacgtttg acggcgcctt
     3961 ttccgtaaaa gtaggcgcgt atgatatggc gtacgacatg atgactgatt ggaccatgag
     4021 ctgtacgtta tggacagtca aatcggcagg cagtcatgtt tcgctcagcg gctcggtgag
     4081 ggaacagaag aatttgcagg gtaacagtga cactacgacc atcaatgtgg acggccgcga
     4141 caacatcggc aatgaaacgt tccgtttcga gaactacatt gtgacggccg tctctgacaa
     4201 cctcctgaat cccacgaccg gt
//
LOCUS       DASSLD010000077         5324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1198538, whole genome shotgun sequence.
ACCESSION   DASSLD010000077 DASSLD010000000
VERSION     DASSLD010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198538"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..2032)
                     /gene="nuoL"
                     /locus_tag="VFK23_01410"
     CDS             complement(104..2032)
                     /gene="nuoL"
                     /locus_tag="VFK23_01410"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET7317769.1"
                     /translation="MIKYAFIPLLPFAGFLIAGLFGKYLKEKAAFFPIAGVLGAFVLA
                     VSAFFDVLGGAKINENLYTWIQVGTLSVNVGFQIDQLTTVMLLVVTTLSSLIHIYSVG
                     YMHGDKGYPRFFAYLALFTFFMLVLVMANNFLLMFVGWEGVGLCSYLLIGFWYEKKSA
                     ADAGVKAFVVNRVGDFGFVLAVLLIFLTFGSLDFSQVFAALGTPAADQTYHLFGGEIS
                     VITLIALLLFLGATGKSAQLPLYVWLPDAMEGPTPVSALIHAATMVTAGVFMVARCAP
                     IFSLSETAMNTVAIVGGLTAVFAATIGLVQNDIKRVIAYSTISQLGYMFLGLGVGAFS
                     AGIFHLTTHAFFKGLLFLASGSVIHALSGEQDMRKMGALKNKIKITFVVFMFGTLALS
                     GIPPFAGFFSKDEILWSAFNAPGMGRFLWFLGAVGAFMTAFYSFRLIYLTFYGKSRLD
                     HEVEHHVHESPKVMTIPLMILAFFSLTIGFIGMPVIEHGNIFGEYLAPLFPHAEHAAE
                     HEGKAAELLAMVVSVAIAVAGWRLASGWYKTGTDWPDRAANRFKGVYNLLLNKYKVDE
                     IYNAIFVNGLVHKMAKFLHSVGDVKIIDGFINGLASAIGSTSESGKKLQSGYVQQYAF
                     TMGLGLVVLVGLYYILIN"
     gene            complement(2134..2436)
                     /gene="nuoK"
                     /locus_tag="VFK23_01415"
     CDS             complement(2134..2436)
                     /gene="nuoK"
                     /locus_tag="VFK23_01415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010029941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET7317770.1"
                     /translation="MVPLSWYILLSAALFSIGVAGVLTRRNVFIILMSIELMLNAANI
                     NLVAFSHYLQSLTGQVFVIFVITVAAAEAAVALAIIILLARNRGSVYVDEFNIMKG"
     gene            complement(2519..3043)
                     /locus_tag="VFK23_01420"
     CDS             complement(2519..3043)
                     /locus_tag="VFK23_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET7317771.1"
                     /translation="MNNMINQLIFLYFAAVILASAVLMVTRRNPVHSVMFMLLLFFHI
                     AGVFVLLNAEFLAAVQLIVYAGAILILYLFVVMLLNVDRESSAARANRFWPWMVAFGA
                     LIAGEVIVLIARGTFPTETDQPLQHAGAGVKELGVLLYQKYMVPFEIASVILLVGLVG
                     AVMLAKKQTSDDRE"
     gene            complement(3036..3524)
                     /gene="nuoI"
                     /locus_tag="VFK23_01425"
     CDS             complement(3036..3524)
                     /gene="nuoI"
                     /locus_tag="VFK23_01425"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HET7317772.1"
                     /translation="MDKIEKFLKTIFLYEIGQGMWVTLKTMFTTQKRKQPYAVTRQYP
                     TFKRKAIPGSRGLHAMRRDDAGKPACIGCGLCEAVCPSKAIKVVTSESPEHLKLVNSY
                     ELDLLRCVFCGFCIDACPVSAILQTPEFELASYSRRDAFYTKDHLVEVGDKFMGRKAG
                     HE"
     gene            complement(3583..4668)
                     /gene="nuoH"
                     /locus_tag="VFK23_01430"
     CDS             complement(3583..4668)
                     /gene="nuoH"
                     /locus_tag="VFK23_01430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HET7317773.1"
                     /translation="MFEMSLMLHYVYAVGIPAVVLLAFILANAMYLQLMERKVLAHMQ
                     GRLGPMRTGWHGILQPIADAVKFMMKEDIIPAKADKWVFSIAPLILLVMAFGAMVMIP
                     FGPPTDFFGLLSNKVPLWVANLNVGVLYILAFSSVGTYGVIMAGWASNNKYSLMGGFR
                     SAAQMISYEIPMAFAILTVVLMAGSLNLTDIVNAQANRWFIVALPVGPVAFYLYLMAG
                     IAETNRVPFDLPEAESELVAGYFTEYSGIRFGIFYMAEYMNMIVVSLLVSIMFLGGWL
                     PVQIFPQTLGHLAHIAFINRMLAAIPPTIWLLGKLYFFIFFYMWLRATLPRYRYDQLM
                     AISWKVLLPVSLGTLLVAAGMKQLGWL"
     gene            complement(4678..>5324)
                     /locus_tag="VFK23_01435"
     CDS             complement(4678..>5324)
                     /locus_tag="VFK23_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7317774.1"
                     /translation="LDMGVTPGHLPGYRKAEKPGLNAVEMMDAIKSGKLTSLYIMGDD
                     PAGSDPGLAAVLGKLEFLVVQDIFMTETAKLAHVVLPAAASAEKTGTFTNLERRLQQL
                     GKAEEPVGESRPDWEIIQDVARRMGGSMNYEGVGDILKEIRTEVPMYAGLAAGSCWAG
                     EKSPLAGTDEDLSLASDSIMKQEVITAERLLFSSGTSITRSHEIGTIRHYKIEV"
BASE COUNT         1177 a         1563 c         1578 g         1006 t
ORIGIN      
        1 atgcaattcg agcatgttaa actcctctaa atacgagttc agtagccgca ggcttcagcc
       61 tgcgcgaatc cgggcgcacc ctgaagggtg cggctacaaa catttaattt atgagtatat
      121 agtacaatcc caccagcacc acaagcccga ggcccatggt gaacgcgtac tgctgcacat
      181 agccgctctg gagcttcttt ccgctctcgc tcgtgcttcc gatggccgag gcaaggccgt
      241 tgataaagcc gtcgatgatc ttgacgtcgc cgacgctgtg gaggaacttc gccatcttgt
      301 ggaccagccc gttcacgaag atggcgttat agatctcgtc caccttgtac ttgttcagga
      361 gcaggttgta gacgcccttg aacctgttgg ccgcccggtc cggccagtcc gtgcccgtct
      421 tgtaccagcc gcttgccagc cgccagccgg caacggcgat ggcaaccgag acaaccatgg
      481 ccaggagctc ggccgctttt ccctcatgct cagccgcgtg ctccgcatga gggaaaagcg
      541 gcgcaagata ttctccgaag atgttcccgt gctcgatcac gggcatgccg atgaagccga
      601 tcgtgagcga gaaaaaggcg agtatcataa gcggtatcgt catcaccttc ggcgactcat
      661 ggacgtgatg ctcgacctca tgatccagcc gtgacttccc gtagaacgtg agatagatca
      721 gcctgaagga atagaacgcc gtcatgaatg cgccgaccgc acccagaaac cagaggaagc
      781 gccccattcc gggcgcattg aaggcgctcc acaggatttc gtccttgctg aaaaaacccg
      841 cgaagggggg gatgccggaa agcgccagcg tgccgaacat gaacacgacg aaggtaatct
      901 tgatcttgtt cttgagcgct cccatcttgc gcatgtcctg ctcgcccgaa agggcgtgga
      961 tcacgctgcc cgaggcgagg aacaggagcc ccttgaagaa ggcatgcgtc gtcaggtgga
     1021 agatgcccgc ggagaacgcc ccgaccccga ggcccaggaa catgtatccg agctggctga
     1081 tcgtggaata ggcgatcacg cgcttgatgt cgttttgcac caggccgatg gtcgcggcaa
     1141 agacggcggt gaggcccccg acgattgcta cggtgttcat ggccgtctcg gaaagcgaga
     1201 agatcggcgc gcagcgcgct accatgaaca cgcccgccgt gaccatggtc gcggcatgga
     1261 tcagggcgct gaccggggtg gggccttcca tggcgtcggg cagccagacg tagagaggaa
     1321 gctgggccga cttgccggtg gcccccagga acagaaggag agctatcagg gtgatgacgc
     1381 tgatctcgcc gccgaacaag tgataggtct ggtccgcggc cggggtcccc aacgcggcga
     1441 agacctggga aaagtcgagg gagccgaagg tcaggaaaat gagcagtacg gccagcacga
     1501 acccgaagtc gcccacgcgg ttcaccacga aggccttgac ccccgcgtcg gccgccgatt
     1561 ttttctcata ccagaacccg atcaggagat aggagcaaag acccacgcct tcccagccca
     1621 cgaacatgag caggaagtta ttcgccatga ccaggacgag catgaagaag gtgaaaagcg
     1681 ccaggtaggc gaagaaccgg ggatagccct tgtccccgtg catatagccc acggaataga
     1741 tgtggatgag ggagctcagg gtcgtaacga cgagcagcat gaccgtggtg agctggtcga
     1801 tctggaaccc cacgttcacc gacaaggtcc cgacctgtat ccaggtgtag aggttctcgt
     1861 tgatcttcgc cccgcccagg acgtcaaaga aggcgctgac ggccaggacg aacgcgccga
     1921 ggacccccgc gatggggaag aacgccgcct tttccttcag gtattttccg aacagtcctg
     1981 cgatcaggaa gccggcgaac ggcaagagcg gtatgaaagc gtatttgatc ataaaagctc
     2041 cgtttattag tcggcagtct gtcggtcgac tgtcgacagt ccgtgataag gattcttggc
     2101 tatcgactta cgactattga ctatcgactg attctatccc ttcatgatat tgaactcgtc
     2161 cacatagacg ctcccgcggt tgcgcgcgag caggatgatg atcgcgagtg ccacggccgc
     2221 ctcggctgcg gcgacggtga tgacgaagat cacgaatacc tggcccgtga gcgactgcag
     2281 gtagtgagaa aacgctacga ggttgatgtt cgccgcgttc agcatgagct cgatggacat
     2341 aaggatgata aagacattgc ggcgagtgag cacgccggcc acgccgatgg agaacagcgc
     2401 ggcgctcaac aatatatacc aggataaggg aaccatggga acctccaaaa ttcagaagac
     2461 ggatgtcaga agtcagacaa cagactaaag cctttggttc ggtcggcctt ctctggctct
     2521 attctctgtc atctgatgtc tgttttttcg ccagcatgac cgcgcccacc agccccacga
     2581 gcaggatgac ggaagctatc tcaaagggca ccatatactt ctgatacagc agcactccca
     2641 gttccttcac accagcgcca gcgtgctgca ggggctgatc ggtctccgtt ggaaaggtgc
     2701 cgcgggcgat caggacgatc acctcgcctg cgatcagcgc gccgaaggcg accatccagg
     2761 gccagaaccg gtttgcccgg gcggcggaac tctcgcggtc gacgttgagg agcatgacga
     2821 cgaaaagata caggatcagg atggcgcccg cgtacacgat gagctggacg gcagccagga
     2881 actcggcgtt caggagaaca aagacgcccg cgatgtggaa gaacaggagc agcatgaaca
     2941 tcacgctgtg cacggggttc cggcgagtaa ccatgaggac cgccgacgcc aggatgaccg
     3001 ctgcaaaata caggaaaata agctggttta tcatattatt catgtcccgc cttcctgccc
     3061 atgaatttat cgcctacctc gacgagatgg tccttggtat agaacgcgtc ccttctcgaa
     3121 tacgaggcca gctcgaactc gggcgtctgc aggatcgccg aaaccgggca tgcgtcgatg
     3181 cagaagccgc agaacacgca ccgaagcagg tccagctcgt aggagttcac gagcttcagg
     3241 tgctccgggc tctcggacgt cacgaccttg attgccttgg acggacagac cgcttcgcac
     3301 aaaccgcacc cgatgcaggc cggctttccc gcatcgtcgc gtcgcatggc atgaagcccg
     3361 cgggacccgg ggatcgcctt ccgcttgaat gtcggatact ggcgcgtcac cgcatacggt
     3421 tgcttccgct tctgggtggt aaacatggtt ttgagcgtca cccacatgcc ctggccgatt
     3481 tcgtacagga atatcgtttt caaaaatttc tctattttat ccatcgtcgt aaactcctcg
     3541 ctgcttcttc cttcttaatt cacacttact taatgtcttt ttctacagcc atcccagctg
     3601 cttcattccg gccgctacca gcagggtccc gagcgaaacc ggaaggagca ccttccagga
     3661 gatcgccatg agctgatcgt atcggtaccg cggcagcgtt gcccggagcc acatatagaa
     3721 gaagatgaaa aagtagagtt tccccaaaag ccagatcgtc ggcggtatgg ccgcgagcat
     3781 cctgttgatg aacgcgatgt gggcgagatg gccgagcgtc tgagggaaga tctgaacagg
     3841 gagccagccg cccaggaaca tgatggaaac caggagcgac acgacgatca tgttcatgta
     3901 ctcggccatg tagaagatgc cgaaccggat gccgctgtac tcggtgaagt accctgccac
     3961 cagttcgctc tcggcctcgg ggaggtcgaa gggaacgcgg ttcgtctcgg cgatccccgc
     4021 catgagatag aggtaaaagg ccacggggcc cacgggcagg gcgacaatga accagcggtt
     4081 ggcctgcgcg ttcacgatat cggtgaggtt cagcgacccg gccatgagaa ccacggtgag
     4141 gatcgcaaag gccatgggga tctcgtagct gatcatctgg gcggcggagc ggaatccgcc
     4201 catcagggaa tacttgttgt tcgaagccca tcccgccatg atcacgccat aggtgccgac
     4261 gctcgaaaac gccaggatgt agaggacgcc gacattcagg ttcgcgaccc agaggggcac
     4321 cttgttcgac aggaggccga aaaagtccgt gggcgggccg aaggggatca tgaccatggc
     4381 gccgaaggcc atcacgagca ggatcagggg cgctatgctg aacacccact tgtctgcctt
     4441 cgccgggatg atgtcctctt tcatcatgaa cttgaccgcg tcggcgatcg gctggaggat
     4501 cccgtgccag ccggtgcgca tcgggccgag acgcccctgc atgtgcgcaa gcaccttgcg
     4561 ttccatcaac tgaagataca tggcgttggc caggatgaag gccagaagca ccacggcagg
     4621 tatgccaacg gcgtagacat aatgaagcat caggctcatt tcaaacatat tctgctccta
     4681 cacttcgatc ttgtaatgcc ggatggtccc gatctcatga gagcgggtga tgctcgttcc
     4741 cgatgagaac agcagccgtt cggcggtgat gacttcctgt ttcatgatgg aatcggatgc
     4801 cagcgaaagg tcttcgtccg tgcccgcgag aggcgacttc tcgcccgccc agcacgagcc
     4861 cgccgcgaga ccggcgtaca tcgggacctc ggtcctgatc tccttcagga tgtcccccac
     4921 cccctcgtag ttcatggacc cgcccatcct cctcgcaacg tcctggatga tctcccagtc
     4981 gggcctggat tcgccgaccg gctcttccgc cttgcccagc tgctggagcc tccgttcgag
     5041 gttggtgaag gtcccggtct tctccgccga tgcggcggca gggagcacga cgtgggcgag
     5101 cttcgcggtc tccgtcatga agatgtcctg cacgacgaga aattcgagct tcccgaggac
     5161 cgctgccagg cccgggtcgc ttcccgcggg atcgtccccc atgatataca gcgaggtgag
     5221 cttgccgctt ttgatcgcgt ccatcatctc gacggcgttc aggcccggct tctccgcctt
     5281 ccggtagccg ggcagatggc ccggcgtcac gcccatgtcg agcg
//
LOCUS       DASSLD010000078         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1100559, whole genome shotgun sequence.
ACCESSION   DASSLD010000078 DASSLD010000000
VERSION     DASSLD010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100559"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="VFK23_01440"
     CDS             complement(<1..1462)
                     /locus_tag="VFK23_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HET7317775.1"
                     /translation="MSRVYKFVITVYRTRRPVYLFFAAIILFSFCSAAMGDDAYVDVL
                     VSTASDTNLQSDRYWDVLLHYKRAGSGKKSLVDDPKFFLASDGKTNPSAELEATIRGF
                     FREDDADDDRPRCRFPARYAWLRERLNIDESRLPPAACRKLDKALDQINPKSTVLIFP
                     AAHGNGPASMFGHTLLRIDSNFKSDLLSYAVNYAAFADDTNGLLYAFKGTFGFYKGFY
                     SILPYYEKVKEYSDMDHRDIWEYYLNLSEDETRRMVLHIWELQNIYSDYYYFDENCSY
                     NLLFLIEAGRPSVDMTDTLKDRMRFWVIPSDTIRAVIDSDLVAGAKYRPSQATRIRTI
                     ASRMATEDQDASLAIIDKKIFPRAVPAMDKASEEKREILDLATEVLQYKYSRREIEKD
                     NYLELFLAVLNARSTLGSNPAELYAPPVPARPEEGHGSGKVGAGVGYAGRDAFLEINL
                     RAAYHDILDPDEGYIEGAEIRFGDVSGRYYFKEDRLR"
     gene            complement(1462..1938)
                     /locus_tag="VFK23_01445"
     CDS             complement(1462..1938)
                     /locus_tag="VFK23_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HET7317776.1"
                     /translation="MRKMFIVAVAVTLSLMLVGSVFAAEKHGAYGAAGCGLGSLVFQD
                     QPGMVQVLAATTNGTFGSQTFGITSGTSNCPQGLKIANNDRLNEFVLANMDNLAKDIA
                     MGKGETLDAFADLLQVPMEKRAEFNEKLQTNFSKIFPSEKVELAGVVDNAVSVSSN"
     gene            complement(2068..>2394)
                     /locus_tag="VFK23_01450"
     CDS             complement(2068..>2394)
                     /locus_tag="VFK23_01450"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7317777.1"
                     /translation="IIDSAFSSYRRIAREKLAKFYLSWFSFLFSDKYSPVDWIAQVSP
                     VPVLVIHGEMDTVVPVHHAEALFDAARPPKELWLVPAEGHVRSFANDIVRQQTVEYLE
                     RVLMTK"
BASE COUNT          558 a          649 c          643 g          544 t
ORIGIN      
        1 gacggagcct gtcctccttg aaataatatc tgccgctcac atcgccgaaa cggatctccg
       61 ctccttctat ataaccttca tcgggatcaa gaatgtcatg gtatgccgcg cgcaaattta
      121 tttccaggaa ggcgtcgcgt ccggcatacc ccactccggc gcccaccttc ccggaaccgt
      181 gtccctcttc cggtcgtgcg gggaccggcg gtgcatagag ctccgcaggg ttgcttccca
      241 aggtgcttcg ggcattgagg accgccagga acagctccag gtaattgtcc ttctcaattt
      301 ccctgcggga atatttatac tggagcacct ccgtagccag atcgagtatc tctctcttct
      361 cctcggaagc cttgtccatg gctggaacag cccggggaaa aatctttttg tcgatgattg
      421 caagcgacgc gtcctggtct tccgttgcca tgcgcgaggc gatcgttcgg atgcgcgttg
      481 cttgggaagg ccggtatttc gctcctgcga ccagatcgct gtcaataacc gcccggatag
      541 tgtcggaagg gatgacccag aacctcatcc ggtctttcag cgtgtccgtc atatcaacgg
      601 acggacgtcc cgcttcgatc aggaagagca ggttgtacga gcagttctca tcaaagtagt
      661 aataatccga atagatattc tggagctccc atatgtggag gaccattctg cgggtctcat
      721 cttcgctcag attgaggtaa tactcccaga tatcgcggtg atccatgtcg ctgtactctt
      781 tgaccttctc gtaatacgga agaatggaat agaacccctt gtagaagccg aaggtacctt
      841 tgaaggcata gagcaggccg ttggtatcgt cggcgaacgc cgcgtaattg accgcgtagg
      901 acaacagatc gctcttgaaa ttgctgtcga tccggagcag cgtgtgtccg aacatcgagg
      961 cggggccgtt gccgtgggcc gcgggaaaga tgagaacagt ggatttggga ttgatctggt
     1021 cgagcgcctt gtcaagtttc ctgcaggcgg ccggaggaag cctcgactcg tcgatgttca
     1081 gcctttccct gagccaggca taccgggcgg gaaatctgca gcggggacgg tcgtcatcgg
     1141 cgtcgtcttc gcggaaaaat cccctgatgg tggcttccag ctctgcggag gggttggtct
     1201 ttccatccga ggccaagaaa aacttcgggt cgtcgacaag actcttcttc cccgagccgg
     1261 cgcgtttgta atggagaagc acgtcccagt agcggtcact ttgcaggttc gtatcactgg
     1321 cggtggagac aaggacgtcg acataggcat cgtcgcccat ggcagcggag cagaaagaga
     1381 acaggataat cgcggcaaaa aaaaggtata caggacgacg cgtcctgtat accgtgatga
     1441 cgaacttata aactcgactc attaattaga ggataccgaa accgcattgt cgacgacacc
     1501 ggccaactcc accttctcgg acgggaaaat cttggagaaa ttcgtctgaa gcttctcgtt
     1561 gaattcagcc cgcttctcca tggggacctg gagcaaatcg gcgaatgcat cgagtgtttc
     1621 gcccttcccc atggcgatgt cctttgcgag gttgtccatg ttcgcgagca cgaactcatt
     1681 cagcctgtcg ttgttggcaa ttttcaaacc ttgcggacag tttgaggtgc ctgaggtgat
     1741 accaaaagtc tgactgccaa atgtcccatt ggtcgtcgcg gcaagtacct gaaccattcc
     1801 cggctggtcc tggaaaacaa gggaaccaag gccgcaaccg gccgcgccgt aggcaccatg
     1861 cttctccgct gcgaacacag aacctaccag catcaaagac aacgttaccg ccactgcaac
     1921 aatgaacatt ttcctcatga aggtcctcct cctgaaattg agattaaaaa ccctgctcca
     1981 ttaaacctaa agcagaggtc tttgtcaagg taaaaaaaat ttcgcttgat atcactgaga
     2041 tatcactgag cagagcggga gggaggatca ttttgtcatt agaacgcgtt ccaaatactc
     2101 tacagtctgc tgtctgacga tatcgttcgc aaacgatcgg acatgtccct ctgccggcac
     2161 cagccacagt tccttcggcg gcctggcggc gtcaaagagc gcttctgcat gatgcacagg
     2221 gacgaccgtg tccatctcgc cgtgaatgac cagcaccggc acgggtgaaa cctgcgcgat
     2281 ccagtccaca gggctgtatt tgtcgctgaa caaaaaagaa aaccaagaca agtagaattt
     2341 cgctagtttt tcgcgggcga tgcgccgata gctcgaaaat gcgctgtcga tgat
//
LOCUS       DASSLD010000079         2907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_969661,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000079 DASSLD010000000
VERSION     DASSLD010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969661"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(392..1762)
                     /locus_tag="VFK23_01455"
     CDS             complement(392..1762)
                     /locus_tag="VFK23_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET7317778.1"
                     /translation="MLIDTHAHLEMREFEDDREDVIGRAREAGVEYIVTIGTTVESSR
                     DAVLLAEKYDFIYAAVGIHPHEVKDILHPAYEILRHFAKHKKVVAYGEIGLDYFYEHS
                     PKSDQKRKFRDMLREARELDLPVIIHDRDAHDDALQIISEEWSPDLGGVMHCFSGDAA
                     MAKKLIELGFSFGVAGPVTFPKAEALREVIRQVPIENLLIETDAPYLAPQPMRGKRNE
                     PAFVRHTAEMIAQIKGLTFDDVSRITSFNAMQLFGIGTVPDRGQITYPIRNSLYLNIT
                     NRCTAACTFCVRYNTDFVKGHNLRLTEEPTADAVIAEIGDPTRYAEIVFCGYGEPLLR
                     LDVVKAVAAEVKRRKGKVRIDTNGHANLIYKRNVLPELAGLIDAVSVSLNAQNAEVYE
                     KLTQPKFGPMTYDAVKEFIGEAKKYIPEVTATVVSAPGVDIEACRKIAEGLGAKFRVR
                     EYNVVG"
     gene            complement(1906..>2907)
                     /gene="metG"
                     /locus_tag="VFK23_01460"
     CDS             complement(1906..>2907)
                     /gene="metG"
                     /locus_tag="VFK23_01460"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF039287.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HET7317779.1"
                     /translation="GDFSTETFTTRFNTELANDLGNLLSRVATMVHKYFDGKMPAAGA
                     ERPSDRELRETAEAVPVRIEEDLTALAFTRYIHDVWTLVTRANRYVEENAPGTLAKKG
                     DMERLGTVLYNLSESLRLIGLYLHPVMPSTSQRIWDALGLERQVASCRLRDEQAWGKT
                     GAGTAVKAGAQLFPRIEKEARNHGSTSPIKSSGDRLMDTDKGKAKEKKVEQTTSGTPA
                     PQPVPAGPELIGIEDFMKADLRVGRIVSAERVAKSEKLVKLQVDIGTETRQVVAGIGK
                     SYTPEELTGKSIIIVANLKPAKLMGVESRGMLLAASSGDLLAVATFDREMKPGAKVK"
BASE COUNT          530 a          854 c          827 g          696 t
ORIGIN      
        1 cgaggctgct acagacaaaa ataagaaaaa gcatatcagt aaaattttca tcattttctc
       61 ctgtgccaac gagtagttaa gccgttaggc cgctgcatct acatatatat gcaagcatct
      121 acaggtatat acagcatcta cctgtagacg ctgtccatgt atatacgggt agatgctttt
      181 ctttgagggc gtgacttcta caacgcatct atatgtatat actttccaag tacccgcaat
      241 tttttcgtag gtggcaacac cgattatcag ggaagtgcta ttgcatgtca aggagttcga
      301 atgggtattt tcggggtatg ttcattttga acggggggac tgttccagga atatgcgtac
      361 tatacaaatg gggctctgac tacctcacgg tttaacccac gacattgtac tcccgcaccc
      421 ggaacttcgc accgagtcct tcggcgatct ttcggcaggc ctcgatgtcc acacccggcg
      481 ctgacaccac cgtagcagtc acttccggaa tgtatttctt cgcctcgccg atgaattcct
      541 tgaccgcgtc gtaggtcatc ggcccgaact tgggctgcgt cagcttctcg taaacttccg
      601 cgttctgggc attgaggctc acggaaactg cgtcgatgag cccagccagc tcgggcagga
      661 cgtttcgttt gtaaatgagg tttgcatggc cgttcgtgtc gatcctgacc ttgcctttgc
      721 gccgcttcac ttcggcggcg acggccttga caacgtcgag ccggaggaga ggttcgccgt
      781 acccgcagaa cacgatctcg gcgtaccgcg tcgggtcgcc gatctcggct atgacggcgt
      841 cggcagtcgg ctcctcggta agcctcaagt tgtggccttt cacgaaatca gtattatagc
      901 ggacacagaa ggtgcaggcg gctgtgcagc ggttcgtgat gttgaggtaa agggaatttc
      961 ttatcggata ggttatctgt ccccggtccg ggaccgtgcc gatgccgaag agctgcattg
     1021 cgttgaagct cgtgatccgt gagacgtcgt cgaaggtgag gcccttgatc tgggcgatca
     1081 tctccgccgt atggcggaca aaggccggct cgttccgctt cccgcgcatg ggctggggcg
     1141 cgaggtacgg tgcgtccgtt tcgatcagga ggttttcgat cgggacctgg cggatgactt
     1201 cgcgcagggc ctcggccttg ggaaaggtga ccggtcccgc gaccccgaag gagaagccca
     1261 gctcgatcag ctttttcgcc atggcggcgt cgccggagaa gcagtgcatg accccgccca
     1321 ggtccggcga ccactcctcg gagatgatct gaagcgcgtc gtcgtgggcg tcgcggtcgt
     1381 ggatgatgac gggcaggtcg agttcccgcg cctcccggag catgtcgcgg aacttcctct
     1441 tctggtcgct tttcggggaa tgttcgtaga agtagtcgag cccgatctct ccgtaggcca
     1501 cgaccttctt gtgcttcgcg aaatgcctga ggatttcgta ggcgggatgg aggatgtcct
     1561 tcacctcgtg gggatggatc ccgacggccg cgtagatgaa gtcgtatttt tccgcgagca
     1621 gcaccgcgtc gcggctcgat tcgaccgtgg tcccgatggt gacgatgtac tcgacgcccg
     1681 cctcccgggc gcgcccgatc acgtcctcgc ggtcgtcttc gaactcgcgc atctccaaat
     1741 gcgcgtgggt gtcgatcagc ataaataaac cctggtgaga agtaagaagt atgaagtaag
     1801 aagtaaggaa aaagccacat tccctgtctc cccacctgtt gttcttgact ccttcttcct
     1861 ccttgttatg aactcttaat tcttaactct tacttcttac ctgcttcact tcactttcgc
     1921 tcccggcttc atctcacggt cgaaagtggc cacggccaga aggtcgccgc ttgaggcggc
     1981 caggagcatg ccccgggact cgacgcccat gagcttggcc ggtttcagat tcgcaacgat
     2041 tataatcgac ttgcctgtga gttcctcggg agtgtagctc ttcccgatgc ccgccacgac
     2101 ctggcgcgtc tccgtgccga tgtccacctg gagcttcacg agcttctcgg actttgccac
     2161 ccgctccgcg gaaacgattc tgcccacgcg gaggtccgcc ttcatgaaat cttcgatccc
     2221 gatcagttcc ggacccgccg ggacgggctg cggcgccggt gttccgcttg tcgtctgctc
     2281 caccttcttc tcctttgctt ttcctttatc ggtgtccata agcctgtccc cggacgattt
     2341 tatcggggat gttgatccgt ggttacgtgc ttctttttcg atccggggaa agagctgcgc
     2401 cccggctttg accgctgttc ccgcgcccgt ttttccccac gcctgttcgt cccgaagccg
     2461 gcacgacgcg acctgccgct ccagcccgag ggcgtcccag atcctctggc tcgtcgacgg
     2521 catgaccgga tggaggtaga gtccgatcag gcggagtgat tccgagaggt tatagagcac
     2581 ggtgcccaga cgttccatgt cccccttctt cgccagcgtc cccggcgcgt tctcctccac
     2641 gtagcggttc gcacgcgtca ccagagtcca gacgtcgtgg atgtatctcg taaatgcgag
     2701 cgcggtaagg tcttcctcaa tcctcacagg gacagcctcc gcggtctcgc gcagttcccg
     2761 gtcggaaggc cgttcagcac cggccgccgg catcttgccg tcgaaatatt tatggaccat
     2821 cgtggcgacg cgcgagagga ggttgccgag gtcgttcgcg agttcggtgt tgaaccgcgt
     2881 cgtaaacgtc tcagtcgaaa aatcgcc
//
LOCUS       DASSLD010000080         2954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_317594,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000080 DASSLD010000000
VERSION     DASSLD010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317594"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VFK23_01465"
     CDS             complement(<1..909)
                     /locus_tag="VFK23_01465"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET7317780.1"
                     /translation="MIHARITGTGSFAPKKVITNHDLERLVDTSDEWITERTGIKERR
                     IAEKGQTTSDLAFEASRRALKAAGLDADELDLILVATMTPDMIMPSAGCVLQDKLGAR
                     KAAAFDIYAACSGFIYGMSVANAFIRSETYRNVLLVGAEVLSRFMDWEDRTTCILFGD
                     GAGAVVLQRYAGKRGILSTHLHSDGSMSDLIHVPAGGSQHPASHDTIHKRMHFIKMKG
                     NETFKAAVRALEGVVQETLEHNRVKSEDIDFLVPHQANLRIIQAMAQRLNMPMEKVVL
                     TLPKHGNTSAASIPMALDVAVRDGRIR"
     gene            complement(906..1919)
                     /gene="plsX"
                     /locus_tag="VFK23_01470"
     CDS             complement(906..1919)
                     /gene="plsX"
                     /locus_tag="VFK23_01470"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET7317781.1"
                     /translation="MKIAVDAMGGDNAPRAVVAGAVQAAREYGAGIVLVGIEQVVREE
                     LEKHPQAKTLPIEVRNATEVVDMLDSAATVFRRKKDSSIRVANELVKSGEADAVFSAG
                     HTGAAMSTSLFVLGRVEGVERPAIATFMPTLSGTSIVLDVGANVDCKPNHLLQFAVMG
                     EVYARYFLRNPNPKVGLLSIGEEETKGNELTKEAFKLLTETSLNFIGNVEGRDLMAGR
                     ADVIVCDGFIGNVVLKVSEAVAEAIGLFLREDIKKSPVSKLGYLLMRPAFRSLKRRVD
                     YAEYGGAPLVGINGISIISHGRSSDRAIKNAIRVAAELAKSDVNKHIREDIEKNMELT
                     RTK"
     gene            complement(2179..2364)
                     /gene="rpmF"
                     /locus_tag="VFK23_01475"
     CDS             complement(2179..2364)
                     /gene="rpmF"
                     /locus_tag="VFK23_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET7317782.1"
                     /translation="MANPKHRHSKSRRDKRRTQKQKLTAPNLAVCPQCQEVKKPHHAC
                     LSCGTYNGREIVTVQQI"
     gene            complement(2450..>2954)
                     /locus_tag="VFK23_01480"
     CDS             complement(2450..>2954)
                     /locus_tag="VFK23_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET7317783.1"
                     /translation="ISDIPPEGLTLELAQNLDLFDLGTATTAFQAVIGIRPASRGILR
                     VTGRIRSSPVLECSRCLENFTFAIDTELDIELAPVGSLGAGAEHELAGGELDTEFYEG
                     DVIEPVDLIKEQLLISIPMVPLHSPECKGLCSVCGTDLNKSECGHQRNAPGDFGAFSA
                     LKDLLKK"
BASE COUNT          519 a          875 c          873 g          687 t
ORIGIN      
        1 ccggatcctg ccgtcccgca ccgctacatc gagggccatg ggaatcgacg ccgccgaggt
       61 gtttccgtgt ttcggaagcg tgagcacgac cttttccatg ggcatgttca gacgctgggc
      121 cattgcctgg atgatcctca ggtttgcctg gtgcggtacg aggaaatcga tgtcttccga
      181 tttgaccctg ttgtgctcca gcgtttcttg aaccactccc tccagggccc gcaccgccgc
      241 cttgaaggtt tcattgccct tcatcttgat gaagtgcatt ctcttgtgga tggtgtcgtg
      301 ggaggccggg tgttgggacc ctccggcggg gacgtggatc aggtcgctca tcgacccgtc
      361 ggaatgcagg tgggtcgaca gaattccgcg cttgcctgcg taccgctgca gcaccaccgc
      421 gcccgcgccg tccccaaaga ggatgcaggt tgtacggtcc tcccagtcca tgaaccgtga
      481 cagaacctcg gcgccgacga gaaggacgtt ccggtacgtt tcggacctga tgaacgcgtt
      541 cgcgacggac atcccgtaaa tgaagccgga acaggcggca tagatgtcga aagcggcggc
      601 tttcctggcg ccaagcttgt cctggagcac gcatcccgcg ctgggcatga tcatgtccgg
      661 cgtcatcgtg gccaccagga tgaggtccag ctcgtccgcg tcgagcccgg ccgccttgag
      721 ggccctccgg cttgcttcaa aggcgaggtc ggacgttgtc tgtcccttct ccgcgatcct
      781 gcgttccttg atgccggtac gctcggttat ccactcgtcg ctggtatcga cgagccgctc
      841 gagatcatgg ttggtgatga ccttcttggg agcgaatgag cccgtccctg ttatccgcgc
      901 atggatcatt tggtcctggt cagttccata ttcttttcga tgtcctcgcg aatgtgtttg
      961 ttgacatcgc ttttggcgag ctcggcagcg accctgattg cgttcttgat cgcacggtcc
     1021 gacgagcggc cgtggctgat gatgctgatg ccgttgatgc cgacgagcgg cgcgccgccg
     1081 tattctgcat agtccacccg gcgtttgagc gaacgaaacg ccggccgcat cagcagatac
     1141 ccgagcttcg aaaccgggct tttcttgatg tcttccctga ggaagagccc gatggcctcc
     1201 gccacggcct cggaaacctt gagtacgacg tttccgatga aaccgtcgca aacgatgaca
     1261 tcggcccttc cggccatcaa atcacgcccc tcgacgtttc cgatgaagtt gagcgaagtt
     1321 tccgtgagga gcttgaacgc ctctttggtc agttcgttgc ccttggtctc ttcctcgccg
     1381 atggacagga ggccgacttt ggggttcggg ttccggagga agtaccgggc gtagacctcg
     1441 cccatgacgg cgaactgcag aaggtgattc ggcttgcagt cgacgttcgc gcccacgtcg
     1501 agcacgatgg aggtcccgct gagcgtcggc atgaaggtcg caatggcggg ccgttcgacg
     1561 ccctcgacgc ggcccagcac gaacagcgac gtcgacattg ccgcgccggt atgtccggcg
     1621 ctgaagacgg cgtccgcctc cccgctcttg acgagttcgt tcgccaccct gatggacgaa
     1681 tcctttttcc tgcgaaagac cgttgccgcg gagtcgagca tgtccacgac ttccgtggcg
     1741 ttccgcacct cgatcggaag cgtttttgcc tgggggtgtt tctccagctc ttcccggacg
     1801 acctgctcga tgccgacaag gacgatgccg gcgccgtact cccgggcggc ctgaaccgcc
     1861 ccggcgacga ccgcgcgcgg tgcgttgtcc cctcccatag catcaacggc aatcttcacc
     1921 gttgagcgcc tttctccgaa actcctcccc ctccggctgc agttacccgt tccgggccgt
     1981 gatgggggaa aaaaggcatt cccccgaacg attctgccgc cgggcggggg tgacgattga
     2041 gaagccgatc aacttcgatg ttcatatacg tactggtgac cgcgggtctg gaccctgaag
     2101 agagattttc cgcaatataa aaggcaaagg ggcttactgc gacaacagta ttatcccttt
     2161 gccttcgaac tacgtccgtt atatctgctg gaccgtgacg atctcgcgac cgttgtaggt
     2221 gccgcatgac aggcaggcat gatgaggctt tttgacttcc tggcactgcg ggcagaccgc
     2281 cagattcggg gccgtgagtt tttgtttttg tgtgcgcctc ttgtcccttc gggatttgga
     2341 atgtctgtgt tttgggtttg ccatgttttc gtagcgctcc ttaatctttt tttgttcaaa
     2401 gacccttcgc ttcgctcagg gtgacgtttt ccacgtatgt gagaaaccgt cattttttca
     2461 gaagatcttt taacgctgaa aatgcaccga agtctcccgg tgcattccgc tgatgaccgc
     2521 actcgctttt gttcagatca gttccacaga cgctgcaaag gcccttgcat tcgggactat
     2581 gaagcgggac catggggatg gagatgagca gctgttcttt tatgagatcg acaggctcga
     2641 ttacgtctcc ctcataaaac tcggtatcaa gttctccgcc ggccagttcg tgctcggcgc
     2701 cggcgccgag ggagcccacc ggggcgagtt cgatgtccag ctccgtgtcg atggcaaagg
     2761 taaagttttc caggcagcgg ctgcattcaa gcacggggga agaacgtatt ctcccggtta
     2821 cgcgaaggat gccgcgcgac gcaggcctga tgccgatgac cgcctgaaac gctgtcgttg
     2881 ccgtgcccag atcgaagaga tcgagattct gagctagttc cagggtgagc ccctctggcg
     2941 gtatgtcgct gatt
//
LOCUS       DASSLD010000081         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_415131,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000081 DASSLD010000000
VERSION     DASSLD010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415131"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VFK23_01485"
     CDS             complement(<1..692)
                     /locus_tag="VFK23_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7317784.1"
                     /translation="MGLKNLKKNLQTEAMPAEPVDPPLAWRMRPRDLSEFAGQEHILG
                     PGKLLRRAIEADRFMSLILYGPPGAGKTALAHLIAEKSRAHFVSLNAVTAGVADIRRV
                     TQEAARERGEGGNRTILFIDEIHRFTRVQQDALLPDVERGNIILIGASTSNPFFSIIP
                     ALSSRSQIFEFKPLGSGDMRNLVLRALADKERGMGNLNVNLTPEALDFIISKSDGDAR
                     RALNALEIGVLST"
     gene            complement(1215..1396)
                     /gene="ssrS"
                     /locus_tag="VFK23_01490"
     ncRNA           complement(1215..1396)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFK23_01490"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1559..1843)
                     /locus_tag="VFK23_01495"
     CDS             complement(1559..1843)
                     /locus_tag="VFK23_01495"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET7317785.1"
                     /translation="MEPVQVEIYGQVYNLKGADNPAHIRELASFVDKKMKDIEKGTGT
                     VDPHRVAILAALTIADELHSLRERFQALETTADKAVSRMLDMTETVEESK"
BASE COUNT          452 a          520 c          527 g          504 t
ORIGIN      
        1 gtggaaagca cgccgatctc gagggcgttc agcgcccgtc tcgcgtctcc gtctgacttg
       61 gagatgatga aatcgagcgc ctccggcgtc aggtttacgt tcaggttccc cattccccgt
      121 tccttatccg cgagcgcccg caggaccagg ttcctcatat ccccggaacc gagcggtttg
      181 aattcgaaaa tctgagagcg ggacgatagc gccggaatga tcgagaagaa ggggtttgac
      241 gtcgacgccc cgataaggat gatgtttccc cgctccacat cgggcagaag ggcgtcctgc
      301 tgcacccggg taaagcggtg gatctcatca atgaagagga tggtcctgtt cccgccctcg
      361 ccgcgctccc tggccgcctc ctgcgtcacc cgccgtatgt cggccacacc tgcggtgacg
      421 gcattgagcg acacgaaatg agcccggctt ttttccgcta tgaggtgggc aagcgcggtc
      481 ttccccgccc ccggcggtcc gtagaggata agcgacatga agcggtcggc ctcgatggcc
      541 ctcctcagga gctttcccgg ccccagaata tgctcctggc ccgcgaactc ggaaaggtcg
      601 cgcggccgca tacgccaggc aagaggcgga tcgacaggct ctgcgggcat ggcttccgtc
      661 tgcaggtttt ttttgaggtt cttgagaccc aaggcgatta catcagggag gaaggcgcgg
      721 cagtgtgata gtgtagtgga cgaaatcgaa gaaatctacc agttcaggta atagcgatgc
      781 ccggggaaac agctagcgtg tgggaacttg gtgaatttcc aggagattga gaaatacggg
      841 tgcacaattc cgacccagtg cccatcgcct ctccattgcc aaatcctggt gttctcctaa
      901 cgagctgttt atatgttgaa ttcgtaaatt tccccatagc ttcgaattgc tgccgagctg
      961 gttcaacgat tttcgcggtc caaagaagtt cgacgttgcg gaagtccgct tggtttaact
     1021 tccttcaggt accgctgttg cttattacta tcacaagcgc cggagccttc cctttttccc
     1081 gctccttatt gacttactgg cgcttttttc tctcttaaag aatatgatga acggtcatta
     1141 tgagtaactt gggatacttg agaggaatga ggctgtttct cgaggtgatg acccgcccac
     1201 gactcataat ataaaaatcc cccgcttttg ccgtgtagat agacttgatt cctggacttt
     1261 gccaggtggg tgccataatg aaaagttcag gcttcccagt ccaatctggg catgcacacc
     1321 cttttcagaa gatagctccc tttaattttt tttcacgagg aacaagttat atccatcacg
     1381 cgcaaagcag gggagatcta tttttttctg tatgtattta taccagaatc agcggaaaat
     1441 ggcaaggata aaagaggctg gaggatggac cggccggcat ggggaacatt ggctttcaat
     1501 gagttacagg gagttacaag ccaggtaaac ccgggaaata agggaattaa caaactgact
     1561 atttcgattc ctccaccgtt tccgtcatat cgagcatgcg actaacggcc ttatctgccg
     1621 tagtttccaa agcctggaac cgctcccgaa ggctgtggag ttcgtcggct atcgtgagcg
     1681 cagccagtat ggctacacga tggggatcga ccgttccagt ccctttctcg atgtctttca
     1741 tttttttgtc gacgaaagat gccagttcgc gtatatgggc gggattgtct gcgcctttga
     1801 ggttgtagac ctgaccgtat atttcgacct gaacgggttc cataaatcaa atccgaaatc
     1861 ctaatatcct ataacatgcc caaattaata aaattcccaa tgacccaacc gctgggaaat
     1921 ccgtgcttgg tgcttgaatt tatatgagat ttggcgtttg ttatctcgtg cttgccctag
     1981 agcagcgcat cgacgaattc ctt
//
LOCUS       DASSLD010000082         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_676125,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000082 DASSLD010000000
VERSION     DASSLD010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676125"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VFK23_01500"
     CDS             <1..684
                     /locus_tag="VFK23_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HET7317786.1"
                     /translation="FSPARILEHWAHVLCFCVLVCTGLTQRFYSLDISQWIILHLGGI
                     DVVRLIHRFAGVVFSLGVCAHMVVAAVGVVYKRWQPSMIITKKDFTDVVHNVRYYLGM
                     ENAPIRGGRYTYKQKFEYWGILTGVLLMMVTGVVLWFPAMVTKIMPGEIIPAAKVLHA
                     NEALVVFLIIAIWHIYNSIFSPEVFPMDTSIFTGYISRERQLDEHPDEFAETEVPPVQ
                     RTPRVALKR"
     gene            957..2726
                     /locus_tag="VFK23_01505"
     CDS             957..2726
                     /locus_tag="VFK23_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET7317787.1"
                     /translation="MVSHYIIKKDIDDLLNKKLALSRLTRNNADGIIRENISRLYDIS
                     ISGAVDLDDGDFRPEKEAAKAAYRYSIFTDGIFLLDNGGNVLLNYPARMRETSLNILS
                     IEPVSRMLALGKPVVSNIYTLEPSQKKVLYVLVPLRDKNGKKVGVAGGQIDPTSPLLL
                     QKLGLLDIGDKEFIDIVDSNGMVIASSNPSRMFTHCDRNSFFTKIIQERKERVASCHV
                     CHEAGDRKEKLSTVLAFVPLETAPWGISVQELKTDVFAPAANLKRLFMILGFIFIGTA
                     LVLTVGISRSIVNPLRDLIKSADRIARGDLSKPIAPEGSDEIGVLSRSFETMRARLVQ
                     SREKLQRYTRELEIRVKERTRQIRESQRRAERLLKKIISTSEDERKRIARELHDGTLQ
                     ELSAALMRVDMCRLHPDQISAEKIDAIRDIIMKTHEGLLETMQNLRPSLLDDLGLIAA
                     VKSLLESHLGERGILYFLNTADVKVAHFRPEVEITLFRIIQEAVGNIARHSQAESVLV
                     HIKCDTIAVYVEIEDDGIGFDLSKLYLNPSDTRDRRGLGILGMKERALLLGGEMEICS
                     QPDLGTRIGVRIPLKNPEVDYAS"
     gene            2716..>2946
                     /locus_tag="VFK23_01510"
     CDS             2716..>2946
                     /locus_tag="VFK23_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7317788.1"
                     /translation="MPHKTKVLIADDHALVREGIAAFLKLSDEFEVVAEASDGLEAIE
                     KTNKHQPDIVIMDINMPKLGGLEATIEIKKMWP"
BASE COUNT          696 a          836 c          810 g          604 t
ORIGIN      
        1 ttttctcccg cccgcatcct cgagcactgg gcgcatgttc tatgcttctg cgtgctcgtc
       61 tgcacgggcc ttacccagag gttttactct ctcgacatct cccagtggat cattcttcat
      121 ttgggaggca tagacgtcgt acgtctcatt caccggttcg cgggggtcgt cttttccctc
      181 ggcgtctgcg cccacatggt tgtcgccgcc gtcggcgttg tctataaaag atggcaaccc
      241 tcgatgatca ttaccaaaaa ggattttacc gacgtcgtgc acaacgtccg gtattacctc
      301 ggtatggaaa acgctcccat ccggggcgga aggtacacgt ataaacagaa gttcgaatac
      361 tgggggatcc tcacgggcgt gcttttgatg atggttacgg gcgtcgtcct ctggttcccc
      421 gcaatggtga cgaagatcat gccgggagag atcatccccg cagccaaggt cctccatgcc
      481 aacgaggccc tcgtggtctt tctgatcatt gcaatctggc atatttacaa ctccattttc
      541 agcccggaag tctttcccat ggatacgagc atcttcacag gctatatttc ccgggagcgc
      601 cagctcgatg aacatcccga cgaatttgcc gaaacggagg tgccgcctgt gcagagaaca
      661 ccccgcgttg cgctgaagag atgagccgag agcacccctt ccccgattca gtcgcctcgt
      721 tgcccgaacg tctcgacaat aatgatcaac ttataacgaa aagcggcaaa caatcgcacg
      781 gccctgcctt gctaatcgta cgaacgacag aaaaggacac tacacagaca gattcttctc
      841 tttttccccg gccggtacga tataatcact ctatgggcag gtaccgcgga aatttgcaaa
      901 aaagaataac catcattatt ctcctaggaa tatcggtcat tctgctgagc ctgggggtgg
      961 tcagtcacta tatcattaaa aaagacatcg acgacctgct caacaagaaa ctcgccctgt
     1021 cccgcctcac gagaaacaac gccgacggca tcatccggga gaatatcagc aggctgtacg
     1081 acatatcgat atcgggcgcg gtcgacctgg atgacggcga tttccgcccg gaaaaggagg
     1141 ccgcgaaagc ggcctaccgg tattccatct tcaccgatgg gatattcctt ctggacaacg
     1201 ggggtaacgt cctgctgaat tatcccgcgc ggatgcggga gacatccctg aacattctca
     1261 gcatcgagcc ggtaagcaga atgcttgcgc tcggaaagcc ggtggtgtcg aacatttaca
     1321 ccctcgagcc atcccaaaaa aaggtcctgt acgtgctcgt gccgctcagg gacaagaacg
     1381 ggaaaaaagt cggtgtggcg ggcgggcaga tcgaccccac cagccccctc ctcctgcaga
     1441 aactgggatt gctcgacatc ggggacaagg agtttatcga tatcgtcgat tcgaacggga
     1501 tggttatcgc ctcttcgaat ccctcacgga tgttcacgca ctgcgaccgg aacagttttt
     1561 tcaccaagat cattcaggag cggaaagaac gggtggcctc ctgccacgtc tgtcatgagg
     1621 ccggagaccg gaaggagaag ctttcgacgg tccttgcctt cgttccgctc gaaaccgcgc
     1681 cttggggcat atccgtccag gaactgaaaa cggacgtgtt cgcccccgcg gcgaacctca
     1741 aaaggctgtt catgatcctg ggcttcatat tcatcggcac ggcgctggtc ctgaccgtcg
     1801 gcataagccg cagcatcgtc aacccgctcc gggacctcat caaaagcgcc gaccgcatag
     1861 cgcgcggcga tttgtcaaaa ccgatcgccc ccgaggggag cgacgagatc ggggtcttga
     1921 gccggagttt cgagaccatg cgggcgcggc tcgttcaatc gagggaaaag ctccagcggt
     1981 atactcggga gctcgagata cgggtcaaag agcggacccg gcagatcaga gagagccaga
     2041 gaagggccga acggctgctg aagaaaatca tctccacctc ggaagacgaa cgaaagcgga
     2101 tcgcgcggga actgcacgat gggacgctcc aggagctttc ggcggcgctc atgcgggtcg
     2161 atatgtgcag gcttcatccc gatcagattt ccgcggagaa gatcgacgcc atccgcgaca
     2221 tcatcatgaa gacccatgaa gggctgctcg agactatgca gaacttgagg ccttccctgc
     2281 tcgacgatct ggggcttatc gccgcggtga aatcgcttct cgaaagccat ctcggggaga
     2341 gaggcattct ctatttcctc aacacggcgg acgtgaaggt cgctcacttc cgcccggagg
     2401 tggagatcac tcttttcagg atcatccagg aggccgtcgg caacattgca cggcattccc
     2461 aggcggaaag cgtacttgtt cacatcaagt gcgatacaat cgcggtttac gtcgaaatag
     2521 aggatgacgg gatcggcttc gacttgagca agctctatct taatccgtcc gacacccggg
     2581 accgcagggg tctgggcatc ctggggatga aagaacgggc gctcctgctt ggaggggaaa
     2641 tggaaatctg ttcgcagccc gacctcggaa cgaggatcgg cgtgcggatc cccttgaaaa
     2701 atccggaggt tgattatgcc tcataaaaca aaagtgctga tcgcggacga tcatgccctt
     2761 gttcgcgaag ggattgccgc attcctcaag ttgagcgatg agttcgaagt cgtcgccgag
     2821 gcttccgacg gccttgaggc catcgaaaaa acgaacaagc accagcccga catcgtcatc
     2881 atggacatca acatgccgaa actcggcggc ctcgaagcga caattgaaat caagaagatg
     2941 tggccg
//
LOCUS       DASSLD010000083         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_186616,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000083 DASSLD010000000
VERSION     DASSLD010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186616"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VFK23_01515"
     CDS             <1..748
                     /locus_tag="VFK23_01515"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET7317789.1"
                     /translation="FPMSRLPLFFISPGQISGSNAVVTGEDVRHIATVLRMKTGDELL
                     LCDGEGTEYAVKIKHVERSGIATEITGRSKREIRSPRITLGQGLPKSDKMDFIVQKAT
                     ELGVSGIVPLVTDRTVVKVKDEEKRTVRWQKIAREAAMQSNRPDIPKVEAIRRLSDFL
                     RTLNSEPGTLLLLPWEEGTVPIKNVLRQNPSVKNVIVLIGPEGGFSKAEADAARTRGF
                     HPVSLGPNILRTETAALTVLSMIGYEYQIP"
     gene            954..1418
                     /gene="rpiB"
                     /locus_tag="VFK23_01520"
     CDS             954..1418
                     /gene="rpiB"
                     /locus_tag="VFK23_01520"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET7317790.1"
                     /translation="MKLAIGCDHGGFELKEEVLKFIKTVANIEVSDLGPAGKESVDYP
                     DYGAKVAEAVSNGAMDRGILICGTGIGMSMVANRYPNVRAALCHDHFTALMSREHNDA
                     NVLVMGERVLGKGVALEIVKTWLDTPFAGGRHQKRLDKITAIEKAVGAATSR"
     gene            1484..>2688
                     /gene="glyA"
                     /locus_tag="VFK23_01525"
     CDS             1484..>2688
                     /gene="glyA"
                     /locus_tag="VFK23_01525"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET7317791.1"
                     /translation="MSELKETDPEVYNAIRGEEERELTKIVLIASENYVSQAVLEAQG
                     SVFTNKYAEGYPRRRYYGGCEFADQVESLAIERAKRIFGAEHVNVQPHSGSSANMAVY
                     MSVLQPGDTILGMGLPHGGHLTHGASVSFSGTLYKSFSYGVDKKTELLNYDEVERLAV
                     EHKPKLIVCGYSAYSRILDFARFRAIADKVGALLMADIAHIAGLVAAGEHPSPVPYAD
                     FVTTTTHKTLRGPRGGMIMCREKFAKAVDKIIFPGIQGGPLVHVIAAKAVAFKEALTP
                     EFGEYQRQVKKNAQALARRLTEDGFRIVSGGTENHLMLVDLTPKNITGRDAETALDLA
                     GITVNKNSIPYDQKPPVVTSGIRLGTPIVTTRGMREPEMKVIGDLICETLANGGAPEK
                     LAAIKERTKA"
BASE COUNT          603 a          817 c          808 g          460 t
ORIGIN      
        1 ttttcccatg tccaggcttc cccttttttt catatccccc ggccagattt cgggctccaa
       61 cgccgtcgtc accggagagg acgtacgcca catcgcaacc gtgcttcgca tgaagaccgg
      121 cgacgagctg ctcctgtgcg acggggaggg gactgagtac gcggtgaaga taaaacacgt
      181 agaacggtcc gggatcgcca cggagatcac cggtcgatcg aaacgtgaga tccgctcccc
      241 acgcatcacc ctcggccagg gactccccaa gtccgacaag atggacttta tcgtacagaa
      301 ggccacggag ctcggcgtat cgggcatcgt accgctcgtc accgaccgga ccgtcgtcaa
      361 ggtcaaggac gaagagaaac ggaccgtgcg atggcagaag atagccaggg aagcggcgat
      421 gcagagcaac agaccggaca ttccgaaagt tgaggcaatc cgtcggctca gcgactttct
      481 ccgaaccctg aactccgaac ccggaactct gcttctcctc ccctgggaag aggggacggt
      541 gccgattaag aatgtcctca ggcagaaccc ttccgtcaag aatgtcatcg tccttatcgg
      601 tcccgaaggc gggttctcga aagctgaagc cgacgcggca aggacccggg ggttccatcc
      661 cgtgagcctt gggccgaaca tcctgcgaac cgagaccgcg gcactaacgg tcctgagcat
      721 gatcgggtat gagtaccaga taccataagc accaaggtcc aagcaccaaa actcaaacaa
      781 attccaaatt tcaaaaggca atatttccaa agggtttcgg ttattggttt tcagcgtttc
      841 gatattattt ggggtttgat attgagaatt tggagctttc ttgcgcccgg tattttcctt
      901 gacacaaaaa cggcggagat gttaatatca tcatctctga aaggcgggtt ttcatgaaac
      961 tggcaatcgg ctgcgatcac ggcggctttg aactgaagga agaagtgctg aaattcatca
     1021 agaccgtcgc gaacatcgag gtctcggacc tcggtccggc gggcaaggaa tccgtcgact
     1081 atccggacta tggcgcgaag gtcgccgagg ccgtgtcgaa cggcgcgatg gaccgcggga
     1141 tcctgatctg cggcacggga atcggcatgt cgatggtggc gaaccggtat cccaacgtcc
     1201 gcgctgccct ctgccacgac catttcacgg cgctgatgag ccgcgagcac aacgacgcga
     1261 acgtccttgt catgggcgag cgcgtcctcg gcaagggcgt ggcgctggag atcgtaaaga
     1321 catggctcga tactccgttc gcggggggca ggcaccagaa gcggctcgac aagattaccg
     1381 ccattgaaaa agccgtcgga gcggcgacta gcaggtagca gcgagcgagg accgtttccc
     1441 gacccttgcc gcttgaccct attattatca gaaggataag gcaatgtcag aactcaaaga
     1501 gacagacccc gaggtctaca acgcgataag gggagaggag gagcgggagc tcaccaagat
     1561 cgtgctcatc gcctcggaga actatgtgag ccaggccgta ctcgaggcgc agggctccgt
     1621 gttcacgaac aagtacgccg agggatatcc ccgccgccgc tactacggcg ggtgcgagtt
     1681 cgcggaccag gtggagtcgc tggccatcga gcgggcgaag cggatcttcg gcgccgagca
     1741 cgtgaacgtc cagccccact ccggctcctc ggccaacatg gccgtctaca tgagcgtgct
     1801 ccagcccggg gacaccatcc tcggcatggg gctcccgcac ggcgggcacc tgacccacgg
     1861 ggcaagcgtc agcttctcgg gtacgctcta caagtctttt tcctacggcg tggacaagaa
     1921 aacggagctc ctcaactacg acgaggtgga gcggctggcc gtggagcaca agccgaaact
     1981 gattgtgtgc ggttacagcg cctattcgcg catcctcgac ttcgcccggt tccgggccat
     2041 cgccgataaa gtcggagccc ttctcatggc ggacatcgcc catatcgcgg gcctcgtggc
     2101 cgccggagag cacccgagcc ccgtgcccta tgcggatttc gtgaccacga cgacccacaa
     2161 gaccctgcgc ggcccccgcg gcggcatgat catgtgcagg gaaaagttcg ccaaggccgt
     2221 ggacaagatc atcttccccg gcatccaggg cgggccgctc gtgcacgtga tcgcggccaa
     2281 ggccgtcgcc ttcaaggagg cgctcacccc ggagttcgga gaataccagc gccaggtgaa
     2341 gaagaacgcg caggcccttg cccggcgcct gacggaagac gggttccgca tcgtgtccgg
     2401 cggcacggaa aaccatctca tgctcgtgga cttgacaccc aagaacatca ccggccgcga
     2461 cgccgagacc gccctcgacc tggcgggcat caccgtgaac aaaaacagca ttccctacga
     2521 tcagaaaccg ccggtcgtga cgagcggcat ccgcctcggc acgcccatcg tcacgacgcg
     2581 cggcatgcgc gagccggaga tgaaggtgat cggcgatctc atctgcgaga cgcttgcgaa
     2641 cggaggcgcg ccggaaaagc tcgctgccat caaggagcgt acgaaagc
//
LOCUS       DASSLD010000084         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_218880,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000084 DASSLD010000000
VERSION     DASSLD010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218880"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFK23_01530"
     CDS             complement(<1..224)
                     /locus_tag="VFK23_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317792.1"
                     /translation="MCIVCAAMLMTSGCATLTTASHFTKDSPKFYSGTRLDIHASAHH
                     EDVLLVYKDKYGVDPPDYPRTDLLFSFLFDT"
     gene            complement(592..1992)
                     /locus_tag="VFK23_01535"
     CDS             complement(592..1992)
                     /locus_tag="VFK23_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7317793.1"
                     /translation="MHNAEKFRAVVVDDEPGVCEAVKAILELEGLAVTTFTDSPAAAE
                     DIKKGAYDLIISDLKMPKMTGLELFDAVRDAAADSIFIIITAFGSIPSAVDAVKKGIY
                     DYIPKPFTPDEVRIPIRRALEKRRLERENLLLKSQIETRYSFRSIIGNSPEIRETFRV
                     MRHAASSESNVLVTGESGTGKELVARAIHSNSVRARRKFVTVNCGAIPDGLIESELFG
                     HVKGSFTSAIADKKGLVEEADKGTLFLDEIGELSTPLQVKLLRVLQEGEFTRIGDIQT
                     GRVDVRVVAATNRDLKKAIADKLFREDLFYRLNVIPIELPPLRRRREDIPLLVNHFIK
                     KHRAKAPEKNITGIDTEGMQTLIGYHFPGNVRELENSIEYAIAFTAGPVIGRNDLPNY
                     ILEGGNIGRDAQKVPISPLKEAKGEFEKSLIIAALIESGGNISEAARLLCVHRQNLQQ
                     KIRLLGIDLASISPER"
     gene            complement(1985..>2539)
                     /locus_tag="VFK23_01540"
     CDS             complement(1985..>2539)
                     /locus_tag="VFK23_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7317794.1"
                     /translation="GDPRRERLKLMARETDRARTIVRQLLNFGREIIIKPEKININRP
                     VTEIIDSLAAQDAFKGIELDLKLADGLPDIYADPAQVGQVALNIMLNAIHSITPPGRI
                     EVATRMDQNYAELVFSDTGGGIPEDHLHKVFDPFFTTKDAYKGTGLGLAVSYGIIKKH
                     GGDISVASRVGKGTTFTVRLPIHA"
BASE COUNT          451 a          728 c          769 g          591 t
ORIGIN      
        1 gtgtcgaaca aaaagctgaa gagaaggtct gttctgggat agtccggagg gtcgacaccg
       61 tacttgtctt tgtaaacgag gagaacatct tcgtggtgcg cgctcgcatg gatgtcgagc
      121 cttgtgccgc tgtaaaactt cgggctgtct ttcgtaaaat gcgaagcggt cgtcaaggtc
      181 gcgcatcccg acgtcatgag catggccgcg catacgatgc acaaactctt catcataatt
      241 tgttaagaat aaggcaaatc gacaaaaaag gcaagaaact cgattgggta ttttatgggt
      301 agggaacgga aacctttgcg gttatgaaag gaagggttca gaattaaaaa aatggtcttt
      361 cgggctttgt atggatagtt tttcccttta ttgttttttg catatcccct tcgcgtcctg
      421 cacgaaattt tggacccgtc taaggccacg tccgctcgat cgcgcaaact cggcctttgg
      481 cctcagacat gcgcgctcgc ccttcgggac gcttccttag gccaagacgg gtgccccaaa
      541 tatttcataa ggtcgctccg gggacatgcg gggttgggtt aaagatcccg ctcaccgctc
      601 cggggagatc gaggcaaggt cgattcccag cagccggatc ttctgctgca ggttctggcg
      661 gtggacgcac aggagcctgg ctgcctcgga gatgttcccg ccggattcga tgagcgcggc
      721 aatgatgagg cttttttcga attcgccctt ggcttccttg agcggcgaga tgggaacctt
      781 ctgggcgtcc ctgccgatgt tcccgccctc gagaatgtaa ttcggcaggt cgttccgccc
      841 gatgaccggg ccggcggtga aggcgatcgc gtactcgatg gaattttcca gctcacgcac
      901 gttcccgggg aaatggtatc cgatcagggt ctgcatcccc tccgtgtcga tgccggtgat
      961 gttcttttcc ggcgctttgg cccggtgttt ttttatgaag tggttcacga ggagcgggat
     1021 gtcttcccgc ctcctcctga gcggagggag ttcgatgggg atcacgttca gccggtagaa
     1081 gaggtcctcg cggaagagct tgtcggcgat cgccttcttc aggtccctgt tcgtggcggc
     1141 aaccacgcgc acgtcgaccc tcccggtctg tatgtcgccg atgcgcgtaa actcaccctc
     1201 ctgcaggacg cgcaggagct ttacctgcag gggggtggag agctcgccga tctcgtcgag
     1261 gaaaagggtc cccttgtccg cctcctccac gagccctttc ttgtccgcga tcgcgctcgt
     1321 gaacgagcct ttcacgtggc cgaacagctc ggactcgatc aggccgtcgg ggatcgcgcc
     1381 gcagttcacc gtcacgaatt tcctgcgcgc ccgcacgctg ttcgaatgga tggcgcgcgc
     1441 cacgagttcc ttgcccgttc cgctctcgcc ggtcacgagc acgtttgact cgctcgacgc
     1501 agcatggcgc atgacgcgga aagtctcacg gatctccggg ctgttgccga tgatgctccg
     1561 gaaggagtac cgggtctcga tctgcgactt gagcaggaga ttttcccgct cgagcctccg
     1621 tttctcgagc gccctcctga ttggtatcct gacctcgtcc ggcgtgaacg gcttggggat
     1681 atagtcgtag atccccttct tgacggcgtc cacggccgag gggatcgacc cgaaggcggt
     1741 gatgatgata aagatactgt cagccgcggc gtcccggact gcgtcgaaaa gctcgagccc
     1801 ggtcatcttc ggcatcttga ggtccgagat gatgaggtcg tatgcgccct ttttgatgtc
     1861 ctcggccgcg gccggactgt ctgtgaaggt cgttaccgcg agcccctcga gttcgaggat
     1921 ggccttcacc gcctcgcaca cccctggttc gtcgtcaacg acgacggccc tgaatttttc
     1981 cgcgttatgc atggatcggc aacctcaccg taaatgtcgt tccctttcct acccggctcg
     2041 cgaccgagat gtcgccgccg tgtttcttga tgatgccgta actgacggca agcccgagcc
     2101 ccgtgccctt gtaagcgtcc ttggtcgtga agaaggggtc gaagaccttg tggaggtggt
     2161 cctccggaat gccgcccccg gtatcggaaa agacgagctc tgcgtaattc tggtccatgc
     2221 gcgtcgcgac ctcgatcctg ccgggcggcg tgatggagtg aatggcgttc agcatgatgt
     2281 tgagggcgac ctggcctact tgagctggat ccgcgtagat atcgggaagg ccgtccgcaa
     2341 gcttgaggtc gagttctata cccttgaacg cgtcctgcgc cgcgagcgaa tcgatgatct
     2401 ccgtaaccgg ccggttgatg ttgatcttct cgggcttgat gatgatctcc cggccgaagt
     2461 tcaggagttg gcgcacgatg gtccgggccc ggtccgtctc gcgcgccatg agcttcagcc
     2521 tttcgcggcg ggggtcccc
//
LOCUS       DASSLD010000085         3659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_552072,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000085 DASSLD010000000
VERSION     DASSLD010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552072"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="VFK23_01545"
     CDS             complement(<1..152)
                     /locus_tag="VFK23_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET7317795.1"
                     /translation="MISLDINGKTYKVDVAPDVPLLWVIRERLKLTGTKFGCGIGMCG
                     SCTVHI"
     gene            complement(485..1516)
                     /locus_tag="VFK23_01550"
     CDS             complement(485..1516)
                     /locus_tag="VFK23_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET7317796.1"
                     /translation="MKLHFTKTNGPIDGAIDKLIESAEGIRRPEYVREMILAALKAGQ
                     EDDESADLKLMNTTLKEMRFTSKVFGPYRDKRKVTVFGSARTQPGEQPYEMARTFGRK
                     LAEAGYMVITGGGGGIMQAANEGAGPEHSFGVNIRLPFEQKPNPVLEGNPRLITYKYF
                     FNRKVAFLKEADAVALFPGGFGTLDEAMETLTLVQTGKRNPLPLVLVDDPVCTYWTEL
                     IKFVEEELLTRNLISKTDLALFERFDSVEAAVARINRFYSRYHSMRYVDGRLVFRLTS
                     PLGPRSVQKLKEKFRDILLPGGDIVSSEALPPEMEDADVVELPRLVLDFNRKDFGRLK
                     SLIDAINDF"
     gene            1584..2048
                     /locus_tag="VFK23_01555"
     CDS             1584..2048
                     /locus_tag="VFK23_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7317797.1"
                     /translation="MSRGKRPPRPGSDILDVSLTKEESAMNYVVLAGRILYVAIFLMA
                     VPGHFTAQTIAYSASMGVPLASMTVPLSGVIALLGGLSILLGYKAKIGGWLIAVFLVP
                     VTLIMHNFWSVQDPMQAMTQQIMFLKNVSMLGAALMIAGVGSGPLSLDSRNK"
     gene            2248..2643
                     /locus_tag="VFK23_01560"
     CDS             2248..2643
                     /locus_tag="VFK23_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317798.1"
                     /translation="MNRILLLLISLLLIAASPLISLASDQTQKSAVSETPQQKAEYEK
                     SMEERLGNIGKGLDELRAKANARTEEVRKELNQYMKDAEKKRDAAARKLEEVRKSTNE
                     TWRKFTSDMNKAADDFEQAYDRAKKRFKE"
     gene            complement(2610..3419)
                     /locus_tag="VFK23_01565"
     CDS             complement(2610..3419)
                     /locus_tag="VFK23_01565"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HET7317799.1"
                     /translation="MNDTHNQGNFAKQLDVARENYYSWHDMPISPPDTQLSLVFSHTE
                     KFLARHLEERLGKPVCLVLTDNSTRMLSARTREGILCVRLHRMFLSSDDPVIDEIASF
                     LKNRRTRMTLFHRFVRDSREQLRRKPPNRITEKTLGKFHDLRVLYRELNEEYFDGMID
                     AAITWGTRSPRYAVRKRTLGSYSERSNTIRINPVLDRKTVPRYYVAFVVYHEMLHAAT
                     GILLRGNRRSVHSREFRKRERDFKDYEKALAWERRARMQPRYSLKRFLALS"
BASE COUNT          771 a          999 c         1067 g          822 t
ORIGIN      
        1 tcgatgtgta ccgtgcagga accgcacatg ccgatgccgc agccgaactt tgtgccggtc
       61 agcttgagcc gttccctgat gacccacaag agcggcacat ccggcgcaac atcgacctta
      121 tacgtcttgc cgttgatatc aagactgatc atggacccac ctcctgttgt caatgagatc
      181 gcagtctgaa aaacctctta atttttccgg ttgtaagggg aagagcgaac ggcgcgggaa
      241 tatcggccgc atgaggaaag cttagcatcg gtgaggggat tgtcaaggaa agaaggggaa
      301 gttcagtaag gcttaagtat tattgaatcg tatatttttt gtcattcccg cgaaagcggg
      361 aatccatttt aaaaaaacca atggatacct gcttccgcag gtatgacagc agcgttggaa
      421 acacagtatg gtcaattcag cgctattatt ctcgaaacac tcactgttac ggtatgagac
      481 cgctctaaaa atcattgatc gcgtcgatca ggctcttcag cctgccgaag tccttgcggt
      541 tgaaatccag gaccagcctc ggcagctcca caacatcggc gtcttccatt tccggcggca
      601 gtgcttcaga cgacacaata tcccctccgg gcagcaggat atcacgaaat ttttccttca
      661 gtttttgaac gcttcgcggt ccgagcggcg aggtgaggcg gaacacgagc ctgccgtcca
      721 catagcgcat gctgtggtag cggctgtaaa agcggttgat cctcgcgacc gccgcctcaa
      781 cggaatcgaa gcgctcgaag agcgcgaggt ccgtcttgct gatgaggttt cgcgtcagaa
      841 gctcttcctc gacgaatttg atgagctcgg tccagtacgt gcacacggga tcgtccacga
      901 gcacgagcgg cagggggttt cgctttccgg tctggaccag cgtgagggtc tccatggcct
      961 catcgagtgt gccgaacccg ccgggaaaga gcgccactgc atcggcctcc ttcagaaagg
     1021 ccaccttgcg gttgaaaaaa tacttatacg taatgagccg cgggttgccc tcgagaacgg
     1081 ggttgggctt ctgctcgaag ggtagccgga tgttgacgcc gaaggaatgt tcggggccgg
     1141 cgccttcgtt ggcggcctgc atgatcccgc ctccgccgcc ggtaatgacc atgtagccgg
     1201 cttcggcgag tttccgtccg aacgttctgg ccatttcgta cggctgttcg cccggctgcg
     1261 tcctggccga tccgaagacc gtgaccttgc gcttgtcgcg gtaggggccg aacaccttgg
     1321 aggtgaaccg catctccttg agcgtcgtgt tcatgagctt gaggtccgcg ctctcgtcgt
     1381 cctcctgtcc ggctttgagc gccgcgagga tcatttcgcg cacatactcc ggccgccgga
     1441 tgccctccgc ggactcgatt aatttatcga tggccccgtc gatggggccg ttcgttttgg
     1501 tgaagtgaag tttcatggtt gtccgttcgc tccttcaaag gcaggggtaa tgcaagaaga
     1561 gataataaga ctttttggca tgtttgtcga ggggaaagag gccgccgcgg cccggttctg
     1621 atatactgga tgtatcactc acgaaggagg aatcggcaat gaactatgtc gttctcgcgg
     1681 gacgaatatt gtatgtggcg atttttctca tggccgtgcc ggggcacttt accgcgcaga
     1741 ccatcgcgta ctcggcgagc atgggcgtgc ccctggcatc gatgaccgtg cccctgtcgg
     1801 gagtgatcgc gcttctgggg ggcctgagca tccttctggg atacaaggcg aagatcggcg
     1861 gctggctgat tgcggtcttt ctcgtccccg tgacgctgat catgcacaac ttttggtccg
     1921 tccaggaccc catgcaggcg atgacgcagc agatcatgtt cctgaagaac gtctccatgc
     1981 tgggggccgc gctcatgatc gccggcgtgg ggtcggggcc cttgagcctt gatagcagga
     2041 acaagtaatc acgaagacga atctttgccg tcattcccga atacagaccc atccccgccc
     2101 tgaccctccc cttgatggcc gatgaatcgg gccacgctga ggggagggaa tttcacggtt
     2161 cctccccctt caagggggag gtgggagggg gatgggttaa tgtggcgatg ttgataatat
     2221 gacgaccttt tgaagggagg ttctgccatg aatcgaatac tactcctgtt gatatcgttg
     2281 ctcctgatcg ctgccagccc gctgatctca ctcgcctctg atcaaactca aaagtcagcc
     2341 gtatcggaaa ccccgcagca gaaggcagag tacgagaaga gcatggaaga gcggctcgga
     2401 aatatcggca aagggctcga cgaactgagg gcgaaggcca atgccaggac cgaagaggtg
     2461 agaaaagagc tgaaccaata catgaaagac gccgagaaga agcgtgatgc cgctgcccgg
     2521 aaacttgagg aggtgcgtaa gtcgacgaac gagacgtgga gaaagttcac ctccgatatg
     2581 aacaaggcgg ccgacgattt cgaacaggcc tatgacaggg ccaaaaagcg cttcaaggag
     2641 taacggggct gcattctagc cctccgttcc cacgccaggg ccttctcgta atctttaaag
     2701 tccctttccc gcttccggaa ttccctcgaa tggacgctcc tcctgttccc ccggaggagt
     2761 atgcccgttg ccgcgtggag catttcgtgg tacacgacga aggccacgta ataccggggg
     2821 accgtcttcc tgtcgagcac cgggttgatc ctgattgtgt tcgaccgctc gctgtagctg
     2881 ccgagggtcc gcttcctcac ggcatagcgc ggacttctcg tgccccaggt gatcgcggcg
     2941 tcgatcatcc cgtcaaagta ctcctcgttg agctccctgt acagaacgcg caggtcgtgg
     3001 aatttcccga gtgttttttc cgttatcctg ttcggcggct tccgtcggag ctgttccctg
     3061 ctgtcgcgga caaaccggtg aaaaagggtc atcctcgttc ttctgttttt caaaaacgag
     3121 gcgatttcgt caatcaccgg atcgtccgaa ctcagaaaca tccgatggag gcgtacgcac
     3181 agaatgccct cgcgcgtacg cgcagagagc atgcgcgttg aattgtcggt gaggacaaga
     3241 caaacgggtt tcccgaggcg ttcttcaaga tgtcgcgcaa gaaatttctc cgtgtggctg
     3301 aagaccaggg aaagttgcgt gtcaggcggg gagatcggca tatcgtgcca actgtaataa
     3361 ttttcacggg ctacgtcaag ttgtttcgca aaattgcctt gattatgcgt atcattcatg
     3421 ttaccgtacc ctccgattgg cccttcaaca taccgctcca cgcttgacga tgacggcagg
     3481 ggagcggcgc agggagcggt aacatgatgt ttgcctttga acgggacgct atcacggccg
     3541 gaaagacccg gcccggcggg gcgcctgatt gtcggtacgt tcgccgtcgg gattctcgtc
     3601 ttcatcacgg tctatggccc tcagatgggc ccctatcgat ccactcggat gcttgcgag
//
LOCUS       DASSLD010000086         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_936311,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000086 DASSLD010000000
VERSION     DASSLD010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936311"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..966
                     /locus_tag="VFK23_01570"
     CDS             40..966
                     /locus_tag="VFK23_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7317800.1"
                     /translation="MRKIVKKKDGIHLERRSFLKGAALGLAGTALPLNKADASFWESF
                     FQKHFKEMNDAEKKQVLSRLEQEYEAKYKKSITVGDEKAMPGVLFGYGLDLSRCIGCR
                     RCEYACVDENNQSKDPQIHWISVLRFKKGEKWVDLEDSEKYYNPELVPEQDYFYMPVQ
                     CQQCENPPCVRACPTQATWKEPDGIVVVDYNWCIGCRYCMAACPYGARHFNWTEPGRK
                     SEEINPQTHYLGNRPRYKGVVEKCTFCIQRTRNGRYPACVEACPVGARKFGNLLDPNS
                     EIRYAIEHKRVFRLKEDLNTNPKFYYFFAYGV"
     gene            978..>2062
                     /gene="nrfD"
                     /locus_tag="VFK23_01575"
     CDS             978..>2062
                     /gene="nrfD"
                     /locus_tag="VFK23_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET7317801.1"
                     /translation="MGTLIKNFFIALRQIFVGTRAYYLWVAFLSCFILLGFSSYVDQL
                     NRGLITSAMRDQVSWGLYISNFTFLVGVAAAAVLLVVPAYLYNFKPIKEIVLFGELLA
                     VTAISMCILFILVDMGQPMRVWHILPIIGAMHFPASLLAWDVVVLNGYLAINGIIAFY
                     VLYRLSIGKEYSMSVIGPLIILSIPWAVSIHTVTAFLYNGLSARPFWNASILAPRFLA
                     SAFCSGPAIMLILFQIIRKRSEVEIDNRAIFKIAELIAYAMGLNLFLLGAEAFKEFYS
                     GSVDSYSIQYLYFGLHGKASLVPWMWAATIMNITAFLLFLTPKTRENFTTLNLGCVLI
                     IMGVYIEKGMGLIVPGFVPDTLGEIYE"
BASE COUNT          433 a          594 c          579 g          456 t
ORIGIN      
        1 gatcatctaa taatactgct gtgtggaacg aggcgaccga tgagaaaaat cgtgaagaaa
       61 aaagacggga tccatctcga gcggaggagc tttcttaaag gtgcggccct cggactggcg
      121 ggcacggcgc tccccctgaa caaggccgat gcctccttct gggaatcgtt ctttcagaag
      181 catttcaagg aaatgaacga tgccgagaag aagcaggtcc tcagccgttt ggagcaggaa
      241 tacgaggcaa agtataaaaa atcgatcacc gtgggcgatg agaaggcgat gccgggcgtc
      301 ctgttcggat acggtctcga tctttcccgg tgcatcggct gcaggagatg cgagtatgcc
      361 tgcgtggacg agaacaacca gtccaaagat ccgcagatcc actggatttc ggtcctcagg
      421 ttcaagaagg gcgagaagtg ggtcgacctc gaggactcgg aaaaatacta caacccggag
      481 ctcgtgccgg agcaggacta tttttatatg ccggtccagt gccagcagtg cgaaaacccg
      541 ccgtgcgtaa gggcctgccc cacgcaggca acctggaaag agcccgatgg gatcgtggtc
      601 gtcgactaca attggtgcat cgggtgccgc tactgcatgg ccgcctgccc gtatggggcg
      661 cgccacttca actggacgga gccgggccgg aagtccgagg aaataaaccc acagacgcac
      721 tacctcggca atcgcccgcg ctacaaggga gtagtagaga agtgtacgtt ctgcatccag
      781 cggacgcgaa acggacgtta ccccgcatgt gttgaggcct gtcccgtggg cgctcggaag
      841 ttcggcaacc tccttgaccc gaacagcgag atccggtacg ccatagagca caaaagggtg
      901 ttccggctca aagaggacct gaataccaac cccaagttct actatttctt cgcatacggt
      961 gtataggagg gttgcccatg ggcaccttga tcaagaactt ttttatcgcc ttgcgtcaga
     1021 tcttcgtcgg gacccgcgcc tattacctct gggtggcgtt tctgtcgtgc ttcatcctcc
     1081 tgggcttcag ctcatacgtg gaccagctca accggggcct catcacgtcg gccatgcggg
     1141 accaggtctc ctggggcctg tatatctcga acttcacgtt cctcgtgggc gtggcggccg
     1201 ccgcggtgct cctggtcgtg ccggcatacc tgtataactt caagccgatc aaggagatcg
     1261 tcctcttcgg ggagcttctg gccgtgaccg cgatctcgat gtgcatcctg ttcatcctgg
     1321 tcgacatggg gcagccgatg cgcgtatggc acatcctgcc gatcatcggc gcgatgcact
     1381 ttcccgcctc cctgctggcc tgggacgtgg tcgttttgaa cggctacctc gccatcaacg
     1441 gcatcattgc cttttatgtc ctctaccgcc tgtcgatcgg gaaggaatac agcatgtccg
     1501 tgatcgggcc cttgatcatt ctctcgatcc cctgggccgt gagcattcat accgtgactg
     1561 cattccttta caacggtttg tcggccagac ccttctggaa cgcatcgatc ctggcgccac
     1621 gctttcttgc ttccgcgttc tgttccgggc ccgccatcat gctgatcctg ttccagatca
     1681 tcagaaaacg gtcggaggtg gagatcgaca accgcgcgat ctttaagatt gcagaattga
     1741 tcgcctacgc catgggcctc aacctgtttc tgctgggcgc ggaggcattc aaggaatttt
     1801 attcaggcag cgttgacagc tactccatcc agtatctgta cttcggtctc cacggaaagg
     1861 ccagtcttgt gccctggatg tgggccgcaa ctatcatgaa catcacggcg tttctcctct
     1921 tccttacccc gaagacccgt gagaacttca cgaccctcaa cctcgggtgc gtcctcatta
     1981 tcatgggcgt gtacatcgaa aagggaatgg ggctgatcgt ccccggcttc gtgcccgaca
     2041 ccctgggcga gatatatgaa ta
//
LOCUS       DASSLD010000087         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_577012,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000087 DASSLD010000000
VERSION     DASSLD010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577012"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..655
                     /locus_tag="VFK23_01580"
     CDS             161..655
                     /locus_tag="VFK23_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013799277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET7317802.1"
                     /translation="MRDVEILIKAGCSPDIVAHCTTVSRIASSLAGRLTVPVDRELVR
                     LGGLFHDIGRSKTHGIEHALAGVEIARRLGFTTALSNIIERHIGAGITASEATRLGLP
                     KKDYLPVTMEEKIVSYADNLVSGTREMPFYEALDRFREILGPDHEGVELFIKQHREIL
                     SWTK"
     gene            705..1613
                     /locus_tag="VFK23_01585"
     CDS             705..1613
                     /locus_tag="VFK23_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET7317803.1"
                     /translation="MKDKKKEMKAVDIEVEPHKKSDSDKVVPVKLTGDSKFKFRCHKG
                     VRCFTACCSNINIVLPPYDLLRLRKRLGMTAEEFINKHCDIEILAKTLLPVITLKMLA
                     DDKKSCPFLTPEGCSVYEDRPNICRYYPVGMATLRNKDAVGGKDEFYFMTREDHCKGF
                     EENKEWTISEWRKDQEADLHDDMNRGWMDILIKKKSFGEREFPEIKNKMFFMASTDTD
                     YFRAFVFESSFLDTYDIPAERVEKVRTDDAELLKLAYEWLRSSMFAEETLKFKEGVIQ
                     ARKERAYAIMKKVYGNKEKLQMGEID"
     gene            complement(1642..>2218)
                     /locus_tag="VFK23_01590"
     CDS             complement(1642..>2218)
                     /locus_tag="VFK23_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317804.1"
                     /translation="HGLYHCESVARDAGAIVLVESEENGILKQDIDTLFTAAIIAGLL
                     HDIKRKEQDHAVRGSIEAERILSDLGMDLRERQYIADAIRNHEAFQETFDRDDPAGRL
                     VSDALYDADKFRWGPENFTTTLWLMVDSHNTPPESLHRVFREKMKGIEKIKETFRTNT
                     GKRYGPEFIDQGITIGNAIYEEMTLLLQEGS"
BASE COUNT          548 a          562 c          635 g          473 t
ORIGIN      
        1 atcccgcggt ttggaatccc ccggctcaag ccgggaagga atgcggtcgg cgcgggagat
       61 tacgttataa aaggagcccc cggcttctga cggagggact cggctccgcg ccgcattcag
      121 tcttgtaatt tgagagaaaa gtggtattat ccagtggttt atgcgtgatg ttgagatatt
      181 aatcaaggca ggctgctctc ctgatatcgt agcccactgc acgaccgttt cccggatcgc
      241 ctcgtcgctt gccggacgcc tcacggttcc ggttgatcgt gagctggtgc ggctgggggg
      301 cctcttccat gacataggca ggtcaaagac ccacggcatc gaacatgcgc tggcaggggt
      361 ggagatcgca aggcgtctgg gatttacaac ggcgctcagt aatatcatcg aacgtcatat
      421 cggggcgggc ataaccgcat cggaggcgac ccggctggga ctgccgaaga aagattacct
      481 gccggtgacg atggaagaaa aaatcgtctc ctacgccgac aacctcgtca gcgggacgag
      541 agaaatgccg ttttatgaag cgctcgaccg gttcagggaa atccttgggc cggaccatga
      601 aggcgtcgag ctgttcataa agcagcatcg ggaaattcta agctggacaa agtgaaccga
      661 attgggaatg cggaataaaa agacaaaaag ctaaagaggg tggcatgaaa gacaagaaga
      721 aagagatgaa ggcagtggat attgaggtag aaccgcataa gaaaagcgac agcgacaaag
      781 ttgtcccggt caaactcacc ggtgacagca agttcaagtt tcgctgccac aagggcgtca
      841 ggtgctttac tgcgtgctgc agcaacatca atatcgtgct gccgccctat gacctcctgc
      901 gtcttcggaa gcggctcggc atgacggccg aggaatttat caacaagcac tgcgatatag
      961 aaattctggc caagacgctc ctcccggtga tcactcttaa aatgttagct gatgataaaa
     1021 aaagctgccc tttcctgaca cccgaaggtt gctcagtata tgaggaccga cccaacatct
     1081 gccgttacta tcccgtgggt atggcaacat tgcgcaacaa ggacgccgtg ggtggcaaag
     1141 acgagtttta tttcatgacc agagaagacc actgcaaagg ctttgaagag aacaaggaat
     1201 ggacgatcag cgagtggcgg aaggaccagg aggctgacct tcacgacgat atgaaccggg
     1261 gatggatgga tatcctgatc aaaaagaagt ccttcggaga gcgggagttc ccggaaatca
     1321 agaacaagat gttttttatg gccagcaccg ataccgatta ttttcgcgcg tttgtattcg
     1381 agagcagctt tctcgacacc tacgacatcc ccgcggaacg cgtcgagaag gtccggaccg
     1441 acgatgcaga actcctgaag cttgcctatg agtggcttag gtcatccatg tttgcggagg
     1501 aaaccctcaa gttcaaagaa ggggtcatcc aggcgcgcaa ggagcgggcc tacgcgataa
     1561 tgaagaaggt atacggaaat aaagaaaagt tgcagatggg ggagattgac tgagagtcaa
     1621 ggagtcggga gaacacatgc tttaactgcc ctcctgcaac agtaaggtca tctcctcata
     1681 gatagcgttt ccaatcgtta ttccctgatc gataaattcc gggccgtacc gcttgccggt
     1741 attggtccgg aatgtttcct tgatcttttc tatgcctttc atcttttccc tgaatacccg
     1801 gtgaagcgat tcggggggtg tgttgtgcga atcaaccatg agccataggg tagtggtgaa
     1861 attctccggt ccccaccgga acttgtcggc gtcgtacagc gcgtcgctga cgagccgccc
     1921 cgccgggtcg tcgcggtcaa aggtttcctg aaacgcctcg tggttcctga tggcgtccgc
     1981 aatatactgc cgctcccgca aatccatgcc gaggtccgac agtattctct ccgcctcgat
     2041 gctgccccgc accgcgtggt cctgctcctt tcgcttgatg tcgtggagca gaccggcgat
     2101 gatcgcggcc gtgaacagag tgtcgatgtc ctgcttcaag atgccgtttt cctcggattc
     2161 cacgaggacg atggcccccg cgtcgcgagc caccgattca cagtggtaaa ggccgtga
//
LOCUS       DASSLD010000088         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1033823, whole genome shotgun sequence.
ACCESSION   DASSLD010000088 DASSLD010000000
VERSION     DASSLD010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033823"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFK23_01595"
     CDS             complement(<1..412)
                     /locus_tag="VFK23_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317805.1"
                     /translation="METINLKKMMKRMESALAATSFAEEGEPEAARSILKEDRRVLLA
                     LKKGQIDAKTLKYALNTAKRISAHLDILFVSTTGRGESKLDPLLEHFVTELRAEKIQY
                     RLIPRTGCLKQQIVDYTNSEKDILFAVIESPHSLD"
     gene            complement(523..699)
                     /locus_tag="VFK23_01600"
     CDS             complement(523..699)
                     /locus_tag="VFK23_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317806.1"
                     /translation="MKSIFKKFETAMVAASFAEEGEFETARQIMNEDRPRKTDRPSNY
                     DYKRPVARKEMRAD"
     gene            963..>2381
                     /locus_tag="VFK23_01605"
     CDS             963..>2381
                     /locus_tag="VFK23_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7317807.1"
                     /translation="MKFFKKSEDEQHEFIIEELRQKISSAHMPPPVAKISGQELELLS
                     RISPSSAEYTIGITYIDYLVSLPWNKKTEDNLDIVRAERILNENHHGLDKIKERIMEH
                     LAIKVLMMNKKARVLVIDDEEIARKNLEHILTKENYDVMTAADGAQALRELESSEFDA
                     VLTDLRMGKVDGMDLLERIKIKYPDTKVIMVTGYATVPSAIEAMKKGAFHYIAKPFKL
                     DEVRDTVKRAVDQKSLRPSTKGSVLCFAGPPGTGKTSLGKSIARALGRKFTRIALGGL
                     KDEADIRGHRRTYVGAKPGRVIEEIRRVESANPVVMLDELDKVGRDFKGDPASALLEV
                     LDPEQNRSFVDHYLDVPFDLSSVMFILTANVLDTIPAPLRDRMEVIEFAGYTQEEKAQ
                     IALRFLCPKQIREKGLSSNQVAFTEGAVKKIIQEYTHEAGIRNLERRIATVCRKIARK
                     TLQSTGGPEVVTISPESVESYLG"
BASE COUNT          593 a          639 c          597 g          552 t
ORIGIN      
        1 catcgagact gtgcggtgat tcgatgacgg cgaaaaggat atctttttcg ctgttcgtat
       61 agtcgacgat ctgctgtttg agacaaccgg tccgcgggat caggcggtat tgtatctttt
      121 ccgccctgag ttccgttacg aaatgctcaa gcaacgggtc cagcttgctc tctcctcgtc
      181 ccgttgtcga aacaaagaga atgtcgagat gagcgctgat cctcttggca gtgtttagag
      241 cgtatttcag ggtcttggcg tcgatctgtc cttttttgag cgcaaggaga acccttcggt
      301 cttcttttag gatagaccgt gctgcctcgg gttcgccctc ctccgcgaag gaggttgctg
      361 ccagcgcgct ttccattctt ttcatcattt tcttaagatt gatcgtttcc atagatagtc
      421 cccgaaactt ctgcatgtta ttgcgaacac ccagcactcg tggtgatctt tatcacgtga
      481 gattgcctgc tttgctcgca aagatgaccc ttgaagcaca tcttagtcgg cacgcatttc
      541 tttccgtgcg acgggccgct tgtaatcata gttggacggc cgatcggtct tcctcgggcg
      601 atcttcattc atgatctggc gggctgtctc aaactcgccc tcttccgcga atgaagctgc
      661 taccattgct gtttcaaatt tcttaaatat gcttttcatg atcattccct ccttctttat
      721 catttgctgt tcactgttct tattacaact accatgccaa agaaaataaa agcattaaca
      781 tattgaaatc atgctattat tgagctgagg ttttaaactg gcttttgttt tgaatgtttt
      841 ataatgcaat aggaatacgc caaattctca ttaactattg aatttacagg cgatatttga
      901 ctgttgcaat aaaaatgaaa acgttgtaaa atgcaaccgt ttcaaaattt cgaaagtacg
      961 ctatgaaatt ttttaaaaaa tcagaagacg aacagcatga attcattatc gaagaacttc
     1021 gccagaagat atcgtcggcg cacatgcccc ctcctgttgc caaaatctct gggcaggaac
     1081 ttgaactcct gagcagaatc agcccctcgt ccgccgaata taccatcggc atcacctaca
     1141 tcgactacct cgtttccctg ccgtggaata agaagaccga agacaatctt gatatcgtcc
     1201 gtgccgagcg catcctgaat gaaaaccacc acggcctgga taagatcaaa gagcggatca
     1261 tggagcatct ggcgatcaaa gtactcatga tgaacaaaaa ggcgcgcgtc ctggtcatcg
     1321 acgacgaaga gatcgcgagg aaaaacctag aacacatcct tacgaaagag aattatgacg
     1381 tgatgacggc agcggacggt gcacaagcgc tccgtgagct tgagtcatcg gagttcgatg
     1441 cggtcctgac cgacctccgc atgggaaaag tcgacggtat ggacctcctg gagcggatca
     1501 agataaaata ccccgacacc aaagtgatca tggtgaccgg ctacgccacc gtcccctcag
     1561 ccatcgaggc gatgaagaaa ggcgcattcc actatatcgc caaaccgttc aagcttgacg
     1621 aagtgcgcga caccgtaaag cgggcggtcg accaaaaatc gctcaggccc agcaccaagg
     1681 gctcggtgct ctgcttcgcc ggccccccgg gcacggggaa gacgtcgctc gggaaatcca
     1741 ttgcccgggc gctcggcaga aaatttacaa ggatcgccct cggcggactg aaggacgagg
     1801 ccgatatccg cggacaccgg aggacctatg tgggcgcaaa gccagggagg gtcatcgagg
     1861 agatccggag ggtggaatcg gccaatcccg tggtcatgct cgacgaactc gacaaggtcg
     1921 gacgcgattt caagggcgac ccggcctcgg cactgctcga agtgctcgat cccgagcaga
     1981 accgctcttt cgtagaccac taccttgacg tcccgtttga tctgtcgagc gtcatgttca
     2041 tcctcaccgc aaacgtgctt gacaccatcc ccgcgccgct ccgtgaccgg atggaggtca
     2101 tcgagttcgc gggctacacg caggaggaga aggcccaaat agccctgcgg ttcctctgcc
     2161 ccaaacagat acgggaaaaa ggcctgtctt cgaaccaggt cgcctttacc gaaggggccg
     2221 taaaaaagat catccaggaa tacacgcatg aagcgggcat cagaaacctc gaacggagaa
     2281 tcgccactgt ctgccgcaag atcgcccgga aaacgctcca gagcacgggg ggcccggagg
     2341 tcgtgaccat aagccctgag tccgtggaat cctatctcgg c
//
LOCUS       DASSLD010000089         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1425216, whole genome shotgun sequence.
ACCESSION   DASSLD010000089 DASSLD010000000
VERSION     DASSLD010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425216"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFK23_01610"
     CDS             complement(<1..265)
                     /locus_tag="VFK23_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317808.1"
                     /translation="MESYRYQQLAYLIVPLTTGVEFFVTARADRRASGKESTGAVIMD
                     VLGYAFAGIIPALFFFTIYTLESNRLPLLAQLLHRLDRYGVMFF"
     gene            440..934
                     /locus_tag="VFK23_01615"
     CDS             440..934
                     /locus_tag="VFK23_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HET7317809.1"
                     /translation="MKRAALIFTWTLLFLLTAGIAALQTTAAVKAWIRPVSTDKCPVC
                     GMFVSKYPDWTAEIVFNDGSYVVFDGAKDMFVFYFDIRKYSPSKKREDIATIYVTDYY
                     AVTSVDARSAYYVSGSDVHGPMGKELVPFAKESDARGFKADHRGRALLTFDAITPDIL
                     KDLK"
     gene            946..1248
                     /locus_tag="VFK23_01620"
     CDS             946..1248
                     /locus_tag="VFK23_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317810.1"
                     /translation="MRKSTAFITTIAFMTALLFAAIFHAQARQRADGPRLLQSEDFVR
                     YFGLTDLCLFTDAQYTRHPAVANRHTPFQDYPLSFEHFPSGSLSGPPPRMVSHEQD"
     gene            1235..>2073
                     /locus_tag="VFK23_01625"
     CDS             1235..>2073
                     /locus_tag="VFK23_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7317811.1"
                     /translation="MSRIEKQRNILDFTLSSLLRRKGKNSALALVYTFIVFMLASVMF
                     FTHSIKTEASLVLRNAPEIVVQRFVAGRQALVPTSYAEKIGRIRGVSAVKGRLWGYYY
                     DPVFGGANYTLLVPEIFPHDDGTVAVGSGVSRNSQSVEGNILPFKGHDGSMATFRIVE
                     VLSSDSELISADLVLMSEGDFRTLFGVKRQYVTDIILSVRNPKEVVTVARKIADILPD
                     TRPIIRDEILRTYDAVFGWRGGILLVIFMGCLAAFVIFAWDRATGLSAEEKKEIGILK
                     AIG"
BASE COUNT          458 a          607 c          562 g          446 t
ORIGIN      
        1 agaagaacat cacaccatac cggtcaagac ggtgaaggag ctgtgcaagc agcggcagcc
       61 gattgctctc aagagtataa atcgtgaaaa agaaaagcgc cggtatgatt ccggcaaacg
      121 catacccgag gacgtccatg atgaccgccc cggttgattc tttgcccgag gcccttctgt
      181 cggcccgcgc agtaacgaag aactcaacgc cggttgtgag cggcacgatc aggtacgcga
      241 gctgctgata ccggtagctt tccatgtaag ttccttcctt ccccgataga cggaaacgaa
      301 caaggtatgg aacccggcag gttgcctacg cagaatatta atgtttccat ccccgcatgt
      361 caaagaaaat tacataaaaa aatgcgaaaa atcggcaaat ttttgatttt tacatgatgt
      421 aatgttataa ttagagccaa tgaaacgcgc agccctcatc ttcacatgga cccttctttt
      481 cctcttgacg gcgggcatcg cagcactgca gacaacagcc gcggtcaagg cctggattcg
      541 ccccgtttcc accgacaagt gtccggtgtg cgggatgttc gtctcgaaat accccgactg
      601 gacggccgaa atagttttca atgacggttc gtacgtcgtg ttcgacggag cgaaggacat
      661 gttcgtgttt tacttcgata tccggaaata cagcccgtca aagaagcggg aggatattgc
      721 gacgatctat gtcacggatt actacgccgt tacctccgtc gatgcgcgga gcgcctacta
      781 tgtgagcgga agcgacgtcc atggtccgat gggcaaggaa ctcgtcccgt ttgcgaagga
      841 gtccgacgcc cggggattca aggcagacca caggggcagg gcgctcctca ccttcgacgc
      901 cataacacca gatatcctga aagacctcaa ataacgggaa ttatgatgag aaaatccacc
      961 gcgtttatta cgacaattgc cttcatgacg gccctgctct tcgccgcaat cttccacgcg
     1021 caggcccggc aacgggccga cgggccccgt cttcttcaga gcgaggattt cgttcgctat
     1081 ttcggactca ccgacctctg cctttttacg gatgcgcagt atacacgaca tcccgccgtt
     1141 gccaaccgtc acactccgtt ccaggactac ccgttgtcct tcgagcattt tccgtcaggg
     1201 tccctgtcgg gtccgccgcc gcgcatggtg agccatgagc aggattgaaa aacagcggaa
     1261 catactcgac tttaccctgt cttcactcct ccggcgaaag gggaagaact cggcgctggc
     1321 gctcgtctac acgttcatcg ttttcatgct tgcgtcggtc atgttcttca ctcattccat
     1381 caagactgag gcgtcgcttg tcctgagaaa cgcgccggaa atcgtcgtgc agcgcttcgt
     1441 tgccggcagg caggcgctcg tgcccacgag ctatgccgag aagatcggca ggatccgggg
     1501 cgtcagcgcc gtcaagggaa ggctgtgggg ctattattat gaccctgtct tcggcggcgc
     1561 aaactatacg ctcctggtcc cggaaatttt cccccacgac gacggcacgg tcgctgtcgg
     1621 cagcggcgtc tcgcggaaca gtcagtctgt cgagggcaac atcctcccct tcaaggggca
     1681 tgacgggtcc atggccacat tccgcatcgt cgaggtgctt tcgtccgatt cggagctgat
     1741 ctcggcggac ctcgttctga tgtccgaagg tgatttccgg acgcttttcg gcgtcaaacg
     1801 gcaatacgtg accgacatca tcctctccgt caggaatccg aaggaggtcg tcaccgtcgc
     1861 ccgcaagatc gcggacatcc tccccgacac ccggcccatc attcgggatg agatcctccg
     1921 cacctacgac gcggtcttcg gctggcgcgg cggtatcctg ctggtgatct tcatggggtg
     1981 cctggcggcg ttcgtcatct tcgcctggga cagggcaaca ggcctctccg ccgaagaaaa
     2041 aaaggagatc ggaatcctga aggccatcgg atg
//
LOCUS       DASSLD010000090         3788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1131329, whole genome shotgun sequence.
ACCESSION   DASSLD010000090 DASSLD010000000
VERSION     DASSLD010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131329"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFK23_01630"
     CDS             complement(<1..189)
                     /locus_tag="VFK23_01630"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET7317812.1"
                     /translation="MIGRNRQSTIDNASTPSPQRSFPGRPTAAVVDLGAIEHNFREVT
                     RRAGGRKVLAVVKAQAYGH"
     gene            complement(288..488)
                     /locus_tag="VFK23_01635"
     CDS             complement(288..488)
                     /locus_tag="VFK23_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317813.1"
                     /translation="MMDPIMCPFCGGTNLFQEDDYSLELDFEYWTIHHWECLDCAQSF
                     DKIDVLPESNSFEAVNHESTIH"
     gene            complement(805..1932)
                     /gene="cobD"
                     /locus_tag="VFK23_01640"
     CDS             complement(805..1932)
                     /gene="cobD"
                     /locus_tag="VFK23_01640"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HET7317814.1"
                     /translation="MQNTEREANECAPSAEGARGQDRVHGGNIYQAAKQYDLELERIL
                     DFSSNVNPLGPSSLAKRAAKRALAYVDRYPDPEMEDLRRAIARYFGIKPEHVVCGNGS
                     NGLIHLIPRVFKPRKVLVPVPTFTEYAAAAKDAGAEVVTVSLKESAGFHMDPVEISFA
                     LKGVDMAFLCNPNNPTGQVVAKQEMLEFMNYAAEYGVTLVVDEAFMDFTESDSVLKET
                     VQTSRIICLRAFTKFFGLPGLRIGYAVSDESTIAALRQGQEPWTVSIPAERAAIAALS
                     DWGHSKKTRRLVEKERSRLLEELRLLPGVETFPGSANFLLIKLVSVDTHQIIERLAYR
                     GILVRDCSSFPGLDSRYIRIAVRTRRENKRLIRTLRELLIV"
     gene            complement(2061..>3788)
                     /gene="ptsP"
                     /locus_tag="VFK23_01645"
     CDS             complement(2061..>3788)
                     /gene="ptsP"
                     /locus_tag="VFK23_01645"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET7317815.1"
                     /translation="VASPGIVIGRAYLLDRRKVVVAGRRIEDVSVKEEVARFKKAVEL
                     SKTQLEDLKKRISKGLGKSHLYILETHIMLLEDRMLVEGTIKRIRESLLNAEGALKET
                     IAAIGIKFDSIEDEYLRDRKHDVEQVGERVLRNLVGHKQESLTEIKEEAIIIAHDLAP
                     SDTLVMRKDKILGFATDAGSRTSHTAILARSLGLPASVGLENITASVKTGDVVILDGI
                     HGVVIVDPDEETFLDYLKKQRKYKYFEQELEKLKSLPAETLDGHIVYLQGNIELPEEA
                     TSTADHGGTGIGLYRTEFLFMNRQTLPTEEEHYAAYRHVAERATPHEVVIRTLDVGGD
                     KIDISGEFEKEANPALGLRAIRFCLKKRDIFRTQLRGILRATAHGNIKMLIPMISGLT
                     ELYETKKMIEDVKAELRAEGRPFNEAVPVGAMIEIPSAAMIADLLAREVDFFSVGTND
                     LIQYTLAIDRQNEHVAYMYEPLDPAVLRLLQRVSEAAHHQKIQLAMCGEMAGDPLYAA
                     ILLGMGFQYLSMNVASIPWVKKVIRAVRMQDAAELATLVMGQPTAALARKTAESFMAE
                     RFPDLMEEL"
BASE COUNT          686 a         1147 c         1125 g          830 t
ORIGIN      
        1 gtggccgtag gcctgggcct tgacgactgc caggaccttc ctccctccgg cgcggcgcgt
       61 cacctcccgg aaattgtgct cgatggcgcc caagtccacc accgccgcgg tcggacggcc
      121 gggaaaggac cgttgcggcg aaggagttga tgcattgtcg attgttgatt gtcgattgcg
      181 acctatcatt gtttaccgct gattgcgatg aagcatcgtt gaccgacggc tgccgattgg
      241 cgatttcagg tttcaatcat cggtcatcaa tcggcaatca tcaatcctca atggatcgtg
      301 ctttcatgat tgaccgcctc gaaagaattc gactcgggca ggacatcgat tttgtcgaac
      361 gactgcgcgc agtctaggca ttcccagtga tggatggtcc agtattcgaa atcaagctca
      421 aggctgtagt cgtcttcctg aaagaggttc gtacccccgc aaaagggaca catgatcggg
      481 tccatcatcg tatcctccga ttttcgtccc tccgcgggca ggcgggagag agaaaatcac
      541 atcccatgcg ccacgggctg cgcgtgcaga atccgcgccg ggcccggacg gcaaaacagg
      601 actgccgcaa cctcgtccgg aataaaatac accgtaattt catgaggttt taattactgt
      661 atcacagaga tggctatttt gcaaaagata ttttcttcga gagggcagtc ttgcgcgcat
      721 gatgtaaaaa aaagaacgcc ctgtccgccg caagggaaaa aggcacggag cagggctttg
      781 ctctggattg tgctattgcg ccgtctatac gatgagcagc tcccggagcg tcctgatgag
      841 ccgcttgttc tcgcgcctgg tccttacggc gatcctgatg tagcggctgt cgaggccggg
      901 gaaggacgag cagtccctga ccaggatgcc gcggtaagcc agccgctcga tgatctggtg
      961 cgtgtcgacg gagacgagtt tgatgaggag gaagttcgcg gagcccggaa aggtctcgac
     1021 gccgggtaga agtcggagct cttcgagcag ccggctgcgt tccttctcga cgaggcggcg
     1081 cgtttttttg ctgtgccccc agtccgagag cgcggcgatc gcggcccgct ccgccggtat
     1141 gctcacggtc cagggttcct gtccctgccg cagggcggcg atcgtcgact cgtcggacac
     1201 cgcgtacccg atacggagtc ccggcaggcc gaaaaacttc gtgaacgccc ggaggcagat
     1261 gatgcgggaa gtctggacgg tctccttgag gaccgaatcg gactccgtga agtccatgaa
     1321 agcctcgtcc acgaccaggg tgacgccgta ttcggccgca tagttcatga actccagcat
     1381 ctcctgtttc gccacaacct ggccggtagg gttgttcggg ttgcacagaa aggccatatc
     1441 gacgcccttg agggcaaacg atatttccac ggggtccatg tgaaacccgg cgctctcctt
     1501 gagcgatacc gtcacgacct ccgcgccggc gtccttcgcc gcggcggcat attccgtgaa
     1561 ggttggaacg gggaccagca ccttccgcgg tttgaaaaca cggggaatga gatggatcag
     1621 gccgttcgag ccgtttccgc agacgacgtg ctccggcttg atgccgaaat agcgcgcgat
     1681 ggctcgtcgg aggtcttcca tctcggggtc cggatagcgg tcgacgtaag cgagggctct
     1741 cttggccgca cgcttcgcca gcgacgacgg ccccagcggg ttcacattgg aactgaaatc
     1801 gaggattctt tccagctcga ggtcgtactg ttttgccgcc tggtagatgt ttccaccatg
     1861 aaccctgtcc tgaccccgcg ctccttcggc gctcggagcg cactcattgg cttccctttc
     1921 cgtattctgc acgctgattt cctttcccaa aatgtggcag atgccgcagt cgatagcgat
     1981 cagccgtcag ctctcagctt tgactgactg ctgatcgccg acggctgatt gccgaaacat
     2041 tatccgcatt ccgaatcgat ttacagttcc tccatcaggt ccgggaaccg ttctgccatg
     2101 aagctctcgg ccgttttcct ggcaagggcg gccgtcggct gtcccatgac gagcgtcgcc
     2161 agttcggccg cgtcctgcat ccggaccgcg cggatgactt ttttgaccca gggaatcgac
     2221 gccacgttca tgctgagata ctgaaacccc atccccagga ggatcgccgc atagagcggg
     2281 tctccggcca tctcgccgca catggccagt tggatcttct ggtgatgggc cgcttccgag
     2341 acgcgctgca gcaagcggag caccgcgggg tcgagcggct cgtacatgta cgcgacatgt
     2401 tcgttctgac ggtcgatggc gagcgtatac tggatgaggt cgttggtccc gacgctgaag
     2461 aaatcgacct cccgcgcgag aaggtcggcg atcatggcag ccgacgggat ctcgatcatc
     2521 gcgccgaccg gcaccgcctc gttgaacggc cggccctcgg cccggagttc tgcctttacg
     2581 tcttcgatca tcttctttgt ctcgtagagc tccgtcaagc cggagatcat cgggataagc
     2641 atcttgatgt tgccgtgggc cgtcgccctc aggatgccac ggagctgggt ccggaaaatg
     2701 tcgcgcttct tcagacagaa cctgatcgcc cgcagcccga gggcggggtt ggcctccttc
     2761 tcgaactctc cggatatatc gatcttgtcc ccgcccacgt ccagggtccg aatgaccact
     2821 tcgtgcggcg tggccctttc cgccacatgc cggtaggcgg catagtgctc ttcttccgtg
     2881 ggcagtgtct gccggttcat gaagagaaac tccgtgcgat agaggccgat gccggtcccg
     2941 ccgtgatccg ccgtcgaagt cgcctcctcg ggcagttcga tgtttccctg aaggtacact
     3001 atgtgaccgt cgagggtctc ggcaggcagc gacttgagct tctcgagttc ttgctcgaaa
     3061 tatttgtatt ttctctgctt cttgagatag tcgagaaacg tctcctcgtc gggatcgacg
     3121 atcaccacgc cgtggatccc gtcgaggatc acaacgtctc cggtcttcac cgaagccgtg
     3181 atgttctcga ggcccaccga cgcgggcagg ccgagcgagc gcgccaggat agccgtatgg
     3241 gaagtccggc tgcccgcgtc ggtcgcgaac ccgaggatct tgtcctttcg catcaccagg
     3301 gtgtcgctgg gcgcgaggtc gtgggcgatg atgatggcct cctccttgat ctccgtcagg
     3361 ctctcctgct tgtggccgac gaggttccgg agcacgcgtt cgcccacctg ctccacatcg
     3421 tgtttccggt cgcggaggta ttcgtcctcg atgctgtcga acttgatccc gatagcggcg
     3481 atggtctcct tgagcgcgcc ctcggcgttc aggagcgatt ccctgatgcg cttgatcgtg
     3541 ccctcgacga gcatcctgtc ttcgagcagc atgatatgcg tctcgaggat atagaggtgg
     3601 gacttgccga ggcccttgct gatccgtttc ttcaggtcct cgagctgggt cttggacagt
     3661 tccaccgcct ttttaaaccg ggcgacctct tccttgacgc tgacatcctc gatccggcgg
     3721 cccgcgacga ccaccttgcg ccggtcgagc agatacgccc tcccgatcac gatccccggc
     3781 gacgctac
//
LOCUS       DASSLD010000091         3297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_223990,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000091 DASSLD010000000
VERSION     DASSLD010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223990"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFK23_01650"
     CDS             complement(<1..331)
                     /locus_tag="VFK23_01650"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7317816.1"
                     /translation="MKKIFTIRDRTDQRVHMWNARRIEEEVGACSDRAIAKYFLDYLP
                     KDAPIPEAGCGLGAWVVYLSERSYDVFGVDDDLQLIERLKEWKPSLKVGWGDVRKLPY
                     ADNALGAV"
     gene            complement(393..1637)
                     /locus_tag="VFK23_01655"
     CDS             complement(393..1637)
                     /locus_tag="VFK23_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004160827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7317817.1"
                     /translation="MKVLMLNTFDEWGGAARAAFRLHRGIRESGIDSRMMVQFKSGDA
                     KDVVCNTHPLVKLTRQAKLVLDSMPVRLYPNRPIHNFSPALLPQMLAGKVSAIDHDLL
                     HLHWLAGGFLRLETLKKFNKPLVWTLHDSWAFTGGCHVPFECVSYRKQCGVCPVLGSS
                     REKDLSRWVWKRKERSFRNLKLTVVTPSRWLSECAKSSTLFREVRVEVIPNGLDLAQF
                     KPVDKRLARELLCLPQDKKLILFGAFGGTGDRNKGFPLLLTALQKMSAGGWKDAAELM
                     VFGGSEPACAVPFGMKTRYLGRLREDISLVLAYAAADILVVPSIMENLSNMVMEAMAC
                     GTPCVAFNQGGMPDLVEHGRTGYLARPFESDDLAAGIGGVLEDDVARREMGQRSRRKV
                     EEDFALDKVAQRYSALYREILK"
     gene            complement(1637..2620)
                     /locus_tag="VFK23_01660"
     CDS             complement(1637..2620)
                     /locus_tag="VFK23_01660"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7317818.1"
                     /translation="MHGWANRDVLSIYHGLRRLKNRVVNLIDPPVVVLIYHRVTVLQT
                     DPHLLAVSPENFRAQMQFLKRSVRVVRFEEVWPGMRERAVAVTFDDGYADNALEALPV
                     LEEVGVPATFFVSTGALGTREEFWWDELERIILGDAGYPDKFYLDDRDYGRIWSTATR
                     DQRLALHSDIHLLAERIGGARREGWLEQLRGWAGLDRIGREANRSLTPEELQTLAASP
                     LTTVGAHTLTHTPLSVLSEDEQRHEIISSKQQLESLLGTEITVFSYPFGRKDHYNRTS
                     VRLCREAGFRKAASNFPGQAHRWTDNYQIPRQLVRNWDVGTFATRMRSFWI"
     gene            complement(2623..>3297)
                     /locus_tag="VFK23_01665"
     CDS             complement(2623..>3297)
                     /locus_tag="VFK23_01665"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7317819.1"
                     /translation="LIRYMTRKKVKVVFNDFSITKPVSTLFGYDRGTPIDRYYIEKFL
                     TGRSQLLRGRVLEIGDSSYSRRFGGDNVTAFEVLHPTSRNRAATIVGDLTDITTLPEA
                     AVDCFICTQTFNFIFDVQKAVQGAHHLLKPGGVLLATVGGISQISRYDMDRWGDYWRF
                     TTASIDRLFRPVFTECVEVECYGNVLAATAFLQGIVVEDLPDPALLDDRDEDYQLVIA
                     IVARKA"
BASE COUNT          682 a         1046 c          886 g          683 t
ORIGIN      
        1 tgactgctcc cagtgcgttg tccgcataag gcaactttcg gacgtcgccc caaccgacct
       61 ttagtgaagg cttccactcc ttgagccttt cgatcaactg gagatcgtcg tcgactccga
      121 agacgtcgta gcttctttcg ctcaagtaga ccacccaagc gcccaacccg catccggcct
      181 ccggaatcgg agcgtccttc ggcaggtaat ccaggaaata cttcgcgatg gcgcggtcag
      241 agcatgcgcc tacctcctct tcgatccggc gcgcgttcca catgtgcacc cgctggtccg
      301 tccgatcccg tatcgtaaag atcttcttca taccggcttc gctctttccg tcccgccctg
      361 cgacgttctc atcgttcacc cggcctattc agtcatttca gtatctcgcg atacagagcg
      421 gaatatcgtt gcgcgacctt gtcgagggca aaatcctcct ccaccttccg tcgggagcgc
      481 tgtcccatct cccgccgtgc cacatcgtct tccagcaccc cgccgattcc tgccgccagg
      541 tcgtccgact cgaacggccg tgccaaatag ccggtccgtc cgtgttctac gaggtccggc
      601 atacctccct ggttgaacgc gacgcaaggt gtgccgcacg ccatcgcctc catgaccatg
      661 ttcgagaggt tctccattat cgacgggaca actaatatat ccgccgcggc atacgccagg
      721 accaggctga tgtcttcacg gagcctgcca agataacgtg tcttcatccc gaagggtacc
      781 gcacaagcag gttccgatcc gccgaacacc atcagttctg cagcatcctt ccagccacca
      841 gcggacattt tctgaagggc cgtcagcaga agaggaaatc ccttgttccg gtccccggtg
      901 ccgccaaacg cgccgaacag gatgagcttc ttatcctggg gaaggcagag cagctccctg
      961 gccaagcgct tgtcgacggg cttgaactgc gccagatcga gcccgttggg gatcacctcg
     1021 acccgaacct cacggaagag ggtgcttgat ttggcgcatt cggacagcca acggctgggg
     1081 gtgacgacgg tcagcttcag gttccgaaaa gatctctcct ttcttttcca gacccaacgg
     1141 gacagatcct tctctctcga cgaccccagc acaggacaga cgccacattg ctttcgataa
     1201 ctgacgcatt caaaggggac atgacagcct ccggtaaaag cccaggaatc gtggagcgtc
     1261 cagaccaatg gcttattgaa tttcttcaac gtttcaagcc gcaggaatcc ccctgccagc
     1321 cagtgaagat gcaggaggtc gtgatcaatg gcggaaacct tccctgccaa catctgcggt
     1381 aacagggcgg gcgaaaagtt atggatcggc ctgttcggat agagccgcac cggcatcgaa
     1441 tcaaggacca gctttgcctg ccgcgtcagt ttcaccaacg gatgcgtgtt gcaaacgaca
     1501 tccttcgcat cgccgctctt gaactggacc atcatccggg aatcgatgcc cgattcccgg
     1561 atcccccggt gcagacggaa tgcggcccgg gcggcaccac cccactcgtc gaaagtatta
     1621 agcatcagga ccttcatcag atccagaaac tcctcatcct cgtagcgaag gtgcccacgt
     1681 cccagttgcg cacgagttgc ctcggtatct ggtagttatc cgtccaacga tgagcctggc
     1741 cggggaaatt cgacgcggct tttcgaaatc ctgcctcgcg gcagaggcgg accgaagtcc
     1801 ggttgtagtg gtcctttctg ccgaaaggat aggagaaaac cgttatctcc gtgccgagca
     1861 ggctttccag ttgttgtttg gacgagataa tctcgtgccg ctgttcgtct tcggaaagaa
     1921 cggacaaagg tgtatgagtc agggtatgcg ctcctaccgt cgtcaagggg cttgcggcaa
     1981 gcgtctgcaa ttcctccggc gtcagggaac ggtttgcctc cctcccgatc ctgtcgagcc
     2041 ctgcccatcc ccggagctgc tccagccacc cttcccgacg cgcccccccg atcctctcgg
     2101 ccagcaggtg gatatcgcta tgcaaggcca gacgctgatc ccgcgtggcg gtagaccaga
     2161 tcctgccgta atcccggtca tccaggtaaa atttatcagg atagccggcg tcccccaaaa
     2221 tgatccgttc caattcatcc caccagaatt cctctcgggt tcccagagcg ccggtgctta
     2281 cgaaaaaggt cgcaggcacc cccacctctt ccagtaccgg aagtgcctcc aaggcattgt
     2341 ccgcatagcc gtcgtcgaag gtcaccgcca ctgcccgctc cctcattcca ggccagacct
     2401 cttcgaagcg gactacgcga acgctccttt tcaggaactg catctgcgcg cgaaagttct
     2461 ccggggagac tgcaagcaaa tgggggtcgg tctgcaagac cgttacccgg tgatagatca
     2521 gcaccaccac cggagggtca atcagattca ccaccctgtt tttcagccgg cgcaacccgt
     2581 ggtaaatcga gagaacgtcg cggttggccc acccatgcat ggtcaggctt tccgcgcgac
     2641 gatggcaatc accaactggt aatcttcgtc acggtcgtcg aggagagcgg ggtcggggag
     2701 atcctcgacg acgatcccct gcaagaaggc cgtcgcggcc aacacgttgc cgtagcattc
     2761 gacctcgacg cattcggtga aaacgggccg gaaaaggcgg tcgatcgagg ccgtcgtgaa
     2821 ccgccagtag tctccccatc ggtccatatc gtaacggctg atctgactga tccctcccac
     2881 cgtcgcaagc agtacgccgc cgggcttcag caaatgatga gccccctgca ccgccttctg
     2941 gacatcaaag atgaagttga aggtttgagt gcagatgaag cagtcgacgg cagcttccgg
     3001 cagggtggta atgtcggtca gatctccaac gatcgtggcc gccctgttcc gggaggtcgg
     3061 gtgcagtact tcgaacgcag tgacgttgtc cccgccaaag cgccggctgt agctgctgtc
     3121 gccaatctcc agcactcggc cgcggaggag ttgggacctg cctgtgagaa atttctcgat
     3181 gtaatagcgg tcgatggggg tgccgcggtc atagccgaag agcgtgctga ctggcttcgt
     3241 gatggaaaaa tcgttgaaca ccaccttgac tttctttctt gtcatgtacc tgatcag
//
LOCUS       DASSLD010000092         2825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_381515,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000092 DASSLD010000000
VERSION     DASSLD010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381515"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..542)
                     /locus_tag="VFK23_01670"
     CDS             complement(231..542)
                     /locus_tag="VFK23_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317820.1"
                     /translation="MKKILTFIFAAMFAMTFGAAFAEDKPGGEVHKAEKAEKVEKAEK
                     TEKAETAGETHKKGKGEKAETHKHKQKGKKKKGEAEKAEHAATPATPATPATPATPAA
                     K"
     gene            1045..2283
                     /locus_tag="VFK23_01675"
     CDS             1045..2283
                     /locus_tag="VFK23_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317821.1"
                     /translation="MLDEGRQTFRFDTFGSEDFWGGTLKLHSAISGTANGGVGAGVSP
                     ATALSVGLKVDLDALPADLVTQLKNGTVNLNDPATTLALLKLNAVVGVTGFFDAAGKL
                     TSLGIQCAFCHSTVDDALVPGIGHRLDGWNNQDLNVGAIVNLSPDLTPVATLLSPALP
                     GITQDTVRTVLQSWGPGKFDAELFMDGKAFNTNQTVNSVVTGTNVPAATLIPPAYGLA
                     GVNLHTSAGWGSVTYWNAFVGNLEMHGKGTFFDPRLNNPTKYPVATAAGFFNVRSNPD
                     LVTSKLASLQMYQLNIPAPTPPAGSFDAAAADRGKLVFNGKGQCATCHVPPLFTEPGW
                     NMHTPPEVGVDSFQADRSPDGRYRTTPLKGLWSHMKRGFYHDGRFATLADVINHYDTL
                     GTLGLTAQEKTDLAEYLKSI"
     gene            complement(2435..>2825)
                     /locus_tag="VFK23_01680"
     CDS             complement(2435..>2825)
                     /locus_tag="VFK23_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317822.1"
                     /translation="SLLFSPKGTLKPSAEADLHKLARVFLAIMKDEHLQSEISLILVI
                     GHVPSSRVQEATAIERMHVASMVGFIQDALFKLEPELGRGYAPYFASAVRSMDPRQGS
                     TAVVEFRIIPSEYLHIEVLEKLEKYSR"
BASE COUNT          590 a          831 c          767 g          637 t
ORIGIN      
        1 atcgtcaagg cacggggttc gttcagcggt ggggtcctcg cagcgaacaa gatcgaaatc
       61 gaaggcgaat agcactgtta tcgcatgagg ggcggccatt cggccgcccc ttttctattg
      121 catgcggtcg ttgactttcg gcaaaaaaaa gtcccgccct tatggggcgg gacttcatta
      181 cagagacgaa tacattttcc cgcgtcggat aataaatccg cggtatgaaa ttacttcgct
      241 gccggagttg ccggagtcgc cggtgttgcc ggagtcgccg gtgttgccgc atgctcggcc
      301 ttctcagcct cgcccttctt cttcttgcct ttttgcttgt gtttgtgcgt ttcggccttc
      361 tcgcctttgc ccttcttatg ggtctcaccg gccgtctcag ccttctcagt cttctcggcc
      421 ttttcaacct tctcagcctt ctcggcctta tggacctcgc ctcccggctt gtcttccgcg
      481 aaggcagcgc cgaacgtcat ggcaaacata gcagcaaaga tgaacgtaag gatcttcttc
      541 atttggattc acctcctttc cgcatatatt gagatagctc aacaacttta tcaaatccga
      601 ctcctcatgt caagcatgac cggggaacgg atgagatgcc ggcgttccag acatgaaatg
      661 cctctatcac atcgatgtcg gcccgcctat ataataggca gtcgtggtat acttacgtat
      721 gtaatcccgc tatcctgaga agaactgctg cagaagtctt aagtatctca tgttatacgg
      781 aggaggtgat actttgggat gagaaaaatt tattaaccgt cgttacaacg tatggatcgg
      841 tttcagttct tgtttcgaga cattcagacc atcactcagg ttaataatcg acgaaaggag
      901 ttttccatga aaaagatcta tctgtatgtc atttgcgcgc tgttgctagc tgctgccggc
      961 tggaccttca cgagctgcca caaaagcagc gcagaatcga ccgccacagg ctcgggggac
     1021 ccgatccggg acaatgcact ccgtatgctg gatgaaggca ggcagacctt ccgcttcgac
     1081 accttcggct ccgaggactt ctggggaggc acgctcaagc tgcattcggc aatatccggt
     1141 actgccaacg gaggtgtggg agccggcgtg agcccggcga cggcgctttc ggtggggttg
     1201 aaagtggacc tcgacgcgct tccggctgat ctcgtaacgc agctcaaaaa cgggacggtc
     1261 aatctgaatg atccggcaac tacgcttgcg cttctcaagc tgaacgcggt agtgggcgta
     1321 acaggcttct tcgacgcggc aggcaagctc acctcgcttg gaattcagtg cgccttctgc
     1381 cattccaccg tggacgatgc actcgtcccc ggaatcggtc atcggcttga cggctggaat
     1441 aaccaggacc tcaacgtggg agcaatcgta aatctgtctc cggacctgac acccgtcgct
     1501 acattgctca gccccgccct gccaggcatc actcaggaca cggtccgtac cgtgcttcag
     1561 agctgggggc cgggcaagtt cgacgcggaa cttttcatgg atggtaaggc attcaatacc
     1621 aaccagaccg tcaatagtgt cgtgactggc acgaatgtgc cggccgccac cctgatcccg
     1681 cccgcgtacg gccttgcagg cgtgaacctc cacacctcgg ccggttgggg ttccgtgacc
     1741 tactggaatg ccttcgtcgg caacctcgag atgcacggca agggaacgtt cttcgatcct
     1801 cgattgaata accccacaaa atacccggtc gccactgcgg ccggcttctt caacgtccga
     1861 agcaatcctg atctcgtcac gtctaaactg gcgtccctcc agatgtatca gctgaacatt
     1921 cccgcgccga ccccgcctgc gggctccttt gacgccgcag ccgcagaccg cggcaagtta
     1981 gtcttcaacg gcaagggaca gtgcgcgacg tgccatgtgc cgccgctctt caccgagccg
     2041 ggctggaaca tgcatactcc gcccgaggtc ggcgtagatt cgttccaggc cgaccggtcg
     2101 ccggacgggc gctaccgaac cacgccgctc aagggcctct ggagccatat gaagaggggg
     2161 ttctatcacg acggcaggtt tgcgacactg gctgacgtga tcaatcacta cgatacgctc
     2221 ggcaccctgg gcctcactgc gcaggaaaaa acggaccttg ccgaatacct gaagtcgatt
     2281 tgacgcgcac acaacgcacc acgcgcggaa attttcgcgc gtggtgcgtg ttatcaatac
     2341 tccatcgttt tggtatggat taaagaagaa ggcaatggca gcatcgtcat tccttgccgg
     2401 tcttttcacg cgggaaaccg gccgtcgcag ccgcctaccg ggagtacttc tccagtttct
     2461 ccagcacttc gatatgcaga tattccgacg ggatgatgcg gaactccacg accgcagtgc
     2521 ttccctggcg ggggtccatc gaccggacgg cggacgcgaa atagggcgcg tatcctcttc
     2581 ccagttcggg ctccagcttg aacagggcat cctgaatgaa ccccaccatg ctggccacgt
     2641 gcattctctc aatggcggtc gcctcttgca ctcgggatga ggggacgtgg ccgatgacca
     2701 ggatgagact tatttcggac tgcaggtgct cgtccttcat gatcgccaga aaaacccggg
     2761 caagtttgtg taaatccgcc tccgccgatg gcttgagtgt tcctttcggg gaaaacagaa
     2821 gggac
//
LOCUS       DASSLD010000093         2700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_514863,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000093 DASSLD010000000
VERSION     DASSLD010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514863"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VFK23_01685"
     CDS             <1..523
                     /locus_tag="VFK23_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET7317823.1"
                     /translation="MLKGSKIASSVKVTNEDVKKYFAEHEEMIATDLHLLGIRFPDEG
                     QARTAYERMKSESFETVANEKIAHAPRGKTVPWDMGYLHWNQIPGEWMDAVYALKKGE
                     VSGVLSSQWSGIYIVKLVDRRKNPDAALERVSSGIMNRLVDMRSREAYSNYLGELKRE
                     ARIVKPEERRKPS"
     gene            520..1815
                     /locus_tag="VFK23_01690"
     CDS             520..1815
                     /locus_tag="VFK23_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317824.1"
                     /translation="MRTHTMLRFKGGGTAASSIFCTVCAFIALLVLVPGNVPAASLAG
                     SSNTYLQGRQELDGKRLIPLFEYLDFNTQDSGPVSAHFGGWYRYDLNQQTTNKDVQYG
                     YLSFKSTAANSMVNLGRIMVAEGVAAERVDGIYARTDLWSNFGVSAFGGSPVETEIDL
                     PGNNIIYGARLSHQYPGLYRIGISALKEEKNSEDFRKEEGVDLWLRPVGKVELLGKSN
                     YNMITKEWMEHSYVLVLGPFANLRLNTTASLINYKSFFTGATSAAFFFQPGVIDPNEK
                     VRTLGEEVSYGVTNNLNVSLDYNAFDYEIAGNAKKFGGNIRYSVMNSGGAGLSIHSTE
                     GDTDLLKYMEYRVYGYKNLGKPDITLDALDVAYKTARNNVKDAYSLTLATLYEVKERL
                     KVGADVEYAKNPDFKKDVRGFLKLNYQFDIGYGKRKRGV"
     gene            1817..2263
                     /locus_tag="VFK23_01695"
     CDS             1817..2263
                     /locus_tag="VFK23_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HET7317825.1"
                     /translation="MIKKTVKTAFLLLLPALAAVLFACAKTATMAAWHPVEVTAPVMC
                     SDCHSDDRSSMNHTPDYGTRHKFYAAQNKQTCGVCHKESFCSDCHAHKEELKPSDKYK
                     NSPERSLPHRGDYLNQHRIDGRINPASCFPCHGRTNNERCKTCHIK"
     gene            2248..2685
                     /locus_tag="VFK23_01700"
     CDS             2248..2685
                     /locus_tag="VFK23_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317826.1"
                     /translation="MPYKIESMVIVALLLVGLASFPGCGTANSQAPFDADAQKHAADW
                     MPQGHRTAVENTGTAACQECHGEDLSGGIAKVSCTRCHLGGALSVHPAAWEGSAILTL
                     HGQYVVDNGSDSCKNTACHGDTLRGVSGPACNSCHSFPGLPDP"
BASE COUNT          655 a          717 c          813 g          515 t
ORIGIN      
        1 catgctgaag gggtcgaaaa tcgcctcctc ggtgaaggtg acgaacgagg atgtcaagaa
       61 atacttcgcc gaacacgagg agatgatcgc gaccgatctc catcttctcg gcattcgatt
      121 tcctgatgaa ggacaggcca ggacggcgta cgaacggatg aagagcgaat cgttcgagac
      181 ggtcgcaaac gaaaaaattg cccacgctcc ccgggggaag accgttccgt gggacatggg
      241 gtatcttcac tggaaccaga ttccgggcga atggatggac gccgtttacg cgttgaaaaa
      301 aggagaagtg agtggcgtgt tgagcagtca gtggagcgga atatacatcg taaagcttgt
      361 cgaccggagg aagaaccccg atgccgccct ggagcgcgtg agctccggca ttatgaaccg
      421 gctggtggac atgcgcagca gagaagcata cagcaattac ctcggcgagt tgaagcgaga
      481 agcaaggatc gtcaagccgg aagaaaggag aaaaccatct tgagaaccca tactatgctc
      541 cgtttcaaag ggggcggcac ggctgcatcc agcatcttct gcaccgtttg tgcgttcatt
      601 gcgctcctgg tacttgtgcc gggtaacgtc ccggcggctt cccttgcagg atcatcgaat
      661 acctacctcc agggccgcca agagctcgac ggtaaaagac ttataccatt atttgaatat
      721 ctcgatttca acactcagga cagcgggccg gtatccgccc acttcggcgg atggtaccgc
      781 tatgacctca atcaacagac gacgaacaag gacgtgcaat acgggtacct gagtttcaag
      841 agcacggcag ccaacagcat ggtgaatctg ggaaggatca tggtggctga aggcgtggcc
      901 gcggagcggg ttgacggcat ctatgcccgg accgacctgt ggagcaactt cggcgtgtcg
      961 gccttcggag ggtcacccgt ggagaccgag atcgacctgc ctggaaacaa catcatctat
     1021 ggcgcgcgtt tgtctcacca gtaccccgga ttgtaccgga tcggcatttc cgcattgaag
     1081 gaggagaaaa acagcgaaga cttccggaag gaggaggggg ttgacctctg gcttcggccc
     1141 gtgggcaagg tggaactcct gggaaaatcg aactataaca tgatcacgaa ggaatggatg
     1201 gaacattcct acgtcctggt actcgggccg tttgcgaacc tgcgtctgaa caccacggcg
     1261 tctctgatca actacaagtc ctttttcacg ggagcgacgt cggccgcgtt ctttttccag
     1321 ccgggcgtca tagacccgaa cgagaaggtc aggaccctgg gagaagaggt ttcgtacggt
     1381 gtgaccaaca acctgaacgt ctccttggac tacaatgctt ttgattatga aatcgccggc
     1441 aatgcgaaaa aattcggagg caacatcagg tacagcgtca tgaactccgg gggggcgggg
     1501 ttgtccatcc acagtacgga aggcgatacg gaccttctca agtatatgga gtaccgcgtc
     1561 tacgggtata agaatctcgg caagcccgac atcacgctcg acgccctcga tgtggcgtac
     1621 aagaccgcca gaaacaacgt gaaggacgcc tattccctga ctctggcgac attatacgaa
     1681 gtgaaggagc ggctcaaagt gggggccgac gtcgagtatg cgaagaaccc cgatttcaaa
     1741 aaggacgtgc ggggattttt aaagctgaac taccagttcg atattggata tggcaagcgg
     1801 aagagggggg tgtagcatga taaagaaaac ggtaaaaacg gcgtttttgc tgcttctgcc
     1861 ggcgctcgcc gccgtgctgt tcgcctgtgc gaagacggcc acgatggcgg catggcatcc
     1921 ggtggaggtc acggcgcctg tcatgtgcag cgactgccac agcgacgacc ggtcgtcgat
     1981 gaaccatacg cctgattacg gcacccgcca caaattctac gcggcgcaga acaaacagac
     2041 ctgcggagtc tgccacaagg aatccttctg ttccgattgc cacgcccata aagaggagct
     2101 caagccgagc gataagtaca agaattcgcc ggaaaggtcg ctgccgcacc ggggtgatta
     2161 tctgaaccag cacaggatcg acggcaggat aaatccggcg tcgtgcttcc cgtgtcatgg
     2221 ccgtacgaat aatgagaggt gcaaaacatg ccatataaaa tagaatcgat ggttatcgtt
     2281 gcactgcttc tcgtagggct ggcatcgttt ccgggctgcg ggaccgcgaa ttcccaggcg
     2341 ccgttcgacg cggacgctca gaagcatgcc gcggactgga tgcctcaggg ccatcgtacg
     2401 gcggttgaaa ataccggtac cgccgcctgc caggagtgcc acggcgagga cttgagcgga
     2461 ggaatcgcga aggtgtcctg tacgcgctgc catctgggcg gcgccctgtc ggttcatccc
     2521 gcagcatggg agggaagcgc catcctcacc cttcacgggc agtacgtggt tgacaacggc
     2581 agcgattcct gcaaaaacac ggcctgtcac ggggacacgc tgcggggcgt ttccggcccg
     2641 gcctgtaaca gctgccatag ctttcctggc ctccctgatc cgtagtgacc ggcagaagga
//
LOCUS       DASSLD010000094         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_903015,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000094 DASSLD010000000
VERSION     DASSLD010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903015"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFK23_01705"
     CDS             complement(<1..270)
                     /locus_tag="VFK23_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HET7317827.1"
                     /translation="MAQNTEQLLIAKGKNDIAVLPSMANRHGLIAGATGTGKTVTLRV
                     LAEQFSRIGVPVFMADVKGDLSGIAKPGGDKPDFAARAKQLGVSNF"
     gene            complement(494..1033)
                     /locus_tag="VFK23_01710"
     CDS             complement(494..1033)
                     /locus_tag="VFK23_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317828.1"
                     /translation="MKKILSFTRIILLALALVPLASSSGEGQMGAPVTHSRDFEKMKG
                     LVGVWEGKADMGKGPEQLKVIYELTSAGTAILERFDAGKPHEMLTVYHDYNGKLSMTH
                     YCSLGNQPHMELRNPGENNLTFVLSEKSPGIASPNETHMHAITIAFDGKDSITSTWTL
                     YDKGAEKSRVVVKLARTKM"
     gene            complement(1201..1407)
                     /locus_tag="VFK23_01715"
     CDS             complement(1201..1407)
                     /locus_tag="VFK23_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317829.1"
                     /translation="MKYGDAFKIDNIMSGLQKELKQGVRAVKKSAIVVKDKTGEITRE
                     GVRQLTALEKKSKEALKKAFRKVA"
     gene            complement(1609..>2370)
                     /locus_tag="VFK23_01720"
     CDS             complement(1609..>2370)
                     /locus_tag="VFK23_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7317830.1"
                     /translation="RKENQLFPALETKGITGPSKVMWALHDDIRGFIKDVRKRVKEGK
                     MEKVAVEALVKMVNDMIYKEEHILFPMTLETLSEEEWAKVRKGEEEIGFAWIKPEAHW
                     KPSAESFQQTYIADKIGSLNLDTGLLTADQVNLMLTHLPIDVSFVNENDEVVYYSATP
                     DRLFPRSPGVIGRRVQNCHPPKSVDMVEKILSSFKAGTKDVAEFWIQMRGKVIHIRYF
                     AVRDQAGAYRGCLEVSQDITNIQKITGQKRLLDWE"
BASE COUNT          461 a          691 c          616 g          602 t
ORIGIN      
        1 gaagtttgag acccccagct gtttcgcgcg ggcggcaaag tccggcttgt ctccgcccgg
       61 cttggcgatc ccggaaaggt cgcccttgac gtcggccatg aacaccggca cgccgatcct
      121 gctgaactgc tccgcgagga cccggagcgt cacggtcttg cctgtgcccg tggcgccggc
      181 gatcagaccg tgccggttgg ccatgctggg cagcaccgcg atgtcgttct tgcccttcgc
      241 gatgagcagt tgttccgtat tctgtgccat aaagtctcct cgtgccgtgt cttgccgggt
      301 acacccggcg gatagaacat gcgaaaacgg aggaaattat tccacaaatc ttttctgaaa
      361 gcaagcgttt cccgtcgccg tcaggccgtc tctgtttact catttcagtg agcgcgtcat
      421 aaaaaacagc cggggcacaa tgccccggcc gtcgttctga tcatgcagca tggtccttcc
      481 cgtgcatgaa cgattacatt ttcgtacgag ctaactttac tacaacgcgg gatttctccg
      541 cccccttgtc gtaaagcgtc caggtgctcg tgatactatc ctttccgtca aaggctatgg
      601 tgattgcgtg catatgggtt tcgttcggcg aggctatgcc cggggatttt tcggacagaa
      661 caaacgtcag attgttctca ccgggattcc gaagctccat gtgaggctgg ttcccgagcg
      721 agcagtagtg ggtcatggac agtttcccgt tgtaatcgtg atagaccgtg agcatctcgt
      781 ggggctttcc cgcgtcgaaa cgctccaaga tggcggttcc tgccgaggtg agttcataga
      841 tgaccttgag ctgctccggc ccctttccca tgtccgcttt cccttcccag acgccgacga
      901 gtcccttcat tttttcaaaa tccctggagt gcgtgacagg agcacccatc tggccttctc
      961 ccgaagagga tgcaagggga accagggcaa gggccaaaag gattatcctg gtaaacgata
     1021 atattttctt catgacttaa cctcctgcgt gcctgtttct ctgttcttct gcctccatat
     1081 tatcacagga cggcatcgcc tggagggcgc atcatcggcg aaaacgtgaa ccaaaacggg
     1141 gctggaggaa ttcagcctcc agccccgatg gtaaggccct tatcgacaag gaccggtaaa
     1201 ttatgcgact ttcctgaacg cttttttaag cgcttctttt gattttttct ccagggcagt
     1261 aagctgccgc acgccttccc gggttatctc tccggtcttg tctttgacga cgatggcgct
     1321 cttcttcacg gcccttacgc cttgtttgag ctctttctgc agaccggaca tgatattgtc
     1381 aatcttgaag gcatctccat atttcatgac aacacctcct gataggatgt tcccgacatc
     1441 ccggactgat cgtcgttagt tttcttgttc ttagataaat cttacctcta ctttttacga
     1501 tcggcaatca agaattcccg gtgcaggtac tctcgacggc caataatgcg aaaaaagtat
     1561 ggcgaaaaac agcgcttgcc ctccagccgg gttacggagc ttcgatcctc actcccagtc
     1621 caacagcctc ttctgccccg tgatcttctg gatattcgtg atgtcctgcg agacttccag
     1681 gcatccgcga tacgcgcctg cttgatcccg cacggcgaag tagcggatgt ggatcacctt
     1741 cccgcgcatt tggatccaga actctgcaac gtccttggtc cctgccttga atgaagagag
     1801 gatcttctcc accatgtcca cgcttttggg gggatggcag ttctgcactc ttctcccgat
     1861 gacgccgggg ctccgcggaa acaagcggtc aggagtcgcc gaatagtaga cgacctcgtc
     1921 gttctcgttc acgaaagaga catcgatagg caagtgcgtg agcatcaggt tgacctggtc
     1981 cgccgtaagc aagccggtat cgaggttcag gctgccgatc ttgtcggcaa tataggtctg
     2041 ctggaatgat tcggcggagg gcttccagtg cgcctcgggc ttgatccagg caaagccgat
     2101 ctcctcctcg ccctttctca cctttgccca ctcctcctcg gaaagggttt cgagggtcat
     2161 cgggaagagg atgtgctctt ccttgtagat catgtcgttc accatcttga cgagggcctc
     2221 gacagccacc ttctccatct tcccttcctt cacgcgcttc ctgacgtcct tgatgaaacc
     2281 cctgatgtcg tcatggagcg cccacatcac cttggacggc ccggtgatac ccttggtctc
     2341 gagggcgggg aaaagctggt tttccttgcg
//
LOCUS       DASSLD010000095         3935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_317620,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000095 DASSLD010000000
VERSION     DASSLD010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317620"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VFK23_01725"
     CDS             <1..799
                     /locus_tag="VFK23_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET7317831.1"
                     /translation="GRWQSEYHDVMIDHDKNIGHLLALLDQLGIANDTVVLYTTDNGP
                     HMNSWPDGGMTPFRSEKNTNWEGAYRVPAMVRWPGHIKPGSVSNEIVSHLDWLPTFVA
                     MAGDPNVKNELMKGFKAGNKTFKVHLDGYNLLPYLTGREAQSPRKEFFYFSDDGDLTG
                     VRYDNWKLVFMEQRLPGTMGVWAEPFVKLRMPKLFNLRTDPYERAVVTSNTYWDWYLD
                     RSFIFYPTADEVGRFLFTFKEFPPRQKAGTWSIGQVLEKIMEGTGGR"
     gene            811..3069
                     /locus_tag="VFK23_01730"
     CDS             811..3069
                     /locus_tag="VFK23_01730"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7317832.1"
                     /translation="MAIHRFIGTFLLGTALVWPVQVAAEGKNVSSFQEHPGYAGSSSC
                     RECHEKFYTLWSSSMHGLAMQPYTSEFAKARLTHPRRDVVIGKLKYRADLDKGVVIET
                     GPKGKKTYPIKHVLGGKNVCYFLTLLPKGRLQTLPVAYDLNKKEWFDMAASGIRHFPG
                     RGTGQGVSWREWPYTFNTACYSCHVSQLSTNYNPETDVYRTIWAEPGINCETCHGPAG
                     EHNKIARATPKGKPLTELRIIRTKTMTREQRNDLCSGCHAKASPLTLEYKPGERFFDH
                     FDLVTLENPDFYPDGRDLGENYTLTSWRMSPCAKSGRIDCMHCHTSSGRYRFKNEKWN
                     DACVPCHADKAGGPAEHTHHPAESVGSRCISCHMPMTSFARMNRSDHSMRPPAPAVTI
                     EYKSPNACNICHTDKDAVWADQHVRKWRTRDYQAPLLERASFIDAARKRNWSRLPEML
                     EYVRSKDRDEVFAASLIRLLMATPDERITPVLLSSMKDPSPLVRSSATEAISVRPTRE
                     GTQALLDATDDGFRLVRIRAAAGLAGYPMDKLTNEVKTRVEKANREYLAFIMARPDQW
                     TSHYNMGNYQLGDGKLKEAVSSYRRALRIEPRAVLAMVNSSIAYSRMGDNGNAEKSLR
                     AALKAVPDNPEANFNMGLLAAEKNDAKGAERYLKAALKYDPQMAQAAYNLCILLSKDR
                     LEGAVRYCRKASELRPDDPKYAYTLAFFLHRKGETAEAVRTLKAITEKHPGYKDAELL
                     LGDITKNKGERP"
     gene            3123..>3935
                     /locus_tag="VFK23_01735"
     CDS             3123..>3935
                     /locus_tag="VFK23_01735"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7317833.1"
                     /translation="MALGLPALAQTDPLPSWNDGPAKQAIIGFVLATADKANPGHVAP
                     EERIAVFDQDGTTWVEQPVYPQAMFAFHQVGVMAANNPELAAAEPFKTVLSGDRASIA
                     RLSMKDIEKILVATHTGMTVEAFQTSVKDWIAKAKHPRWNRLFTELVYQPMLEVMKYL
                     RDNGYKTYLVTGGGQDFVRAFSEQVYGIPPEQVVGSAGATKFGYDGNGRATLTKAPKV
                     VLFDDKAGKPTGIHLMIGRRPVAAFGNSTGDQQMLEYTQAGGGRRLMMLVQHD"
BASE COUNT          875 a         1188 c         1180 g          692 t
ORIGIN      
        1 gggacgctgg cagtccgagt atcacgatgt gatgatcgat cacgacaaaa acattgggca
       61 cctgctcgcc ctgctcgacc agctcggcat cgcaaacgac accgtcgtcc tctacacgac
      121 cgacaacggg cctcacatga actcctggcc cgacggcggc atgacgcctt tccgcagcga
      181 gaagaacacc aactgggagg gcgcctaccg cgtgcccgcc atggtgcgct ggcccggcca
      241 catcaagccg ggatcggtct ccaatgaaat cgtcagccac ctggactggc tgccaacctt
      301 cgtggcgatg gcgggtgatc cgaacgtgaa aaatgaactc atgaagggat tcaaggccgg
      361 aaacaagacg ttcaaggtgc acctcgacgg ctacaacctc cttccctacc tcaccggccg
      421 ggaggcgcag agtccgcgga aggagttctt ctacttctcc gatgacggcg acctgaccgg
      481 tgtgcgctac gacaactgga agctggtctt catggaacag cgcttgccgg gcacaatggg
      541 cgtctgggct gagccttttg tcaagctgcg catgccgaag ttgttcaacc tgcggacgga
      601 tccgtacgaa cgggcggtgg tcacgtcgaa cacgtactgg gactggtacc tggatcgctc
      661 gttcatcttt tatcccactg cggacgaggt ggggcgtttc ctgttcacat tcaaggaatt
      721 tcccccgcgg caaaaagcgg gaacctggtc catcggccag gtgcttgaaa agattatgga
      781 aggaaccggg gggcgctgac cggatacaag atggcgattc atcgattcat agggacgttt
      841 cttctcggca cggcgcttgt ctggccggta caggttgccg ccgagggaaa aaatgtctct
      901 tctttccagg agcatcccgg gtatgccggt tcctccagct gcagggagtg ccatgaaaaa
      961 ttctatacgc tgtggtcttc ctcgatgcac ggactggcca tgcagcccta cacatctgaa
     1021 tttgccaagg ccaggctcac gcatccccgg agagacgttg ttatcgggaa actcaaatac
     1081 cgtgccgatc ttgataaagg cgtggtcatc gagaccggtc cgaaagggaa aaagacgtat
     1141 cccataaaac atgtcctggg tggaaagaat gtctgctatt tccttacgct tcttccgaaa
     1201 ggccggcttc agaccctgcc ggtagcctac gatctcaata aaaaagaatg gttcgacatg
     1261 gccgccagcg gcatccgcca ctttccgggg aggggaacgg gccagggggt cagctggagg
     1321 gaatggccct acaccttcaa taccgcgtgc tacagctgcc acgtaagtca gctctcgacc
     1381 aattataacc ctgaaaccga cgtctaccgc accatctggg ccgagccggg catcaactgc
     1441 gagacttgcc acggtcctgc cggcgaacac aacaagatcg ccagggctac gccaaaaggc
     1501 aagccgctca cggagctccg gatcatccga accaagacca tgacccggga gcagcgcaat
     1561 gacctctgtt cgggctgcca cgcgaaggca agcccgctga ccttggaata caagccgggg
     1621 gagcggttct ttgaccactt cgatctcgtg acgttggaaa accccgattt ttatccggat
     1681 gggcgcgacc tcggggagaa ctacacattg acatcctggc gcatgagccc ctgcgccaaa
     1741 tcgggccgga tcgactgcat gcattgccat acgtcgagcg gccgctatcg gttcaaaaat
     1801 gaaaaatgga acgatgcgtg cgtgccctgc catgcggata aagcgggcgg ccccgcggag
     1861 cacacgcacc atcccgcgga aagcgtgggg agcaggtgca tctcctgcca catgcccatg
     1921 acctctttcg cccgcatgaa ccggagcgat cactcgatgc ggccaccggc gcccgcggtg
     1981 actatcgaat acaaatcacc gaacgcctgc aacatctgcc acaccgacaa ggatgccgtt
     2041 tgggccgacc agcacgttcg caagtggcgc actcgcgact accaggcgcc attgctcgag
     2101 cgggcatcgt tcatcgatgc ggcccgcaaa cggaactggt ccaggctgcc cgagatgctc
     2161 gagtatgtcc gtagcaagga tcgcgacgag gtctttgccg cctccctcat ccggctcctg
     2221 atggcgactc cggacgagcg gatcaccccg gtcttgctct cctccatgaa agacccgtcg
     2281 ccgctggtgc gctcgtcggc aaccgaggcg atctccgtca ggccgacccg tgagggtacg
     2341 caggcgctgc tggatgccac tgacgacgga ttccggctcg tacggattcg cgccgcggca
     2401 ggcctggcgg gatatcccat ggacaaactt acaaatgagg tcaaaaccag ggttgagaaa
     2461 gccaacaggg aatatctcgc cttcatcatg gcccgacccg accagtggac ttcccattac
     2521 aacatgggca actaccagct cggcgacgga aagctcaagg aggccgtttc ttcctaccgg
     2581 agggcgctca ggatcgagcc acgggcggtc cttgcgatgg tgaactcgtc cattgcctat
     2641 tcacggatgg gagacaacgg caatgccgag aaatccctgc gggcggcact caaggcggtg
     2701 ccggacaatc cggaggcgaa cttcaacatg gggctcctgg cggcggagaa aaacgatgcg
     2761 aagggggcgg aaagatattt gaaagccgcc ctgaaatacg atccgcagat ggctcaggcc
     2821 gcatacaacc tttgcatcct tctctcgaag gatcgtctgg aaggagcggt ccgttattgc
     2881 aggaaggcga gcgagctgcg acccgatgat ccgaagtatg catacaccct tgcattcttc
     2941 ctgcaccgga agggcgagac ggccgaggcc gtcaggacgc tcaaggcgat cacggagaag
     3001 catccgggat acaaagatgc cgagttgctt ctcggggaca tcacgaaaaa caagggggaa
     3061 agaccatgag gaataaacgc gcagcaacag gggcatccat cggaatcgtg atttgtctgg
     3121 tgatggcgct gggtctcccg gccctggcgc agacagaccc gcttccgtcc tggaacgatg
     3181 gaccggcgaa gcaggcgatc atcgggttcg tgctcgccac cgccgacaag gcaaatcccg
     3241 ggcacgtggc gcccgaagag cgcatcgcgg tgttcgacca ggacggcacc acctgggtcg
     3301 agcagccggt ctatcctcag gcgatgttcg cattccacca ggtcggcgtg atggcggcga
     3361 ataatccgga gctggcggcg gccgaaccgt tcaagaccgt gctctccggt gatcgggcgt
     3421 ccatcgccag actgtcgatg aaggacatcg agaagatcct cgtagctacc cacaccggca
     3481 tgaccgtgga ggcgttccag acatccgtaa aggactggat cgccaaggcg aagcacccac
     3541 gctggaaccg gctcttcacc gagctcgttt atcagccgat gctcgaagtg atgaagtacc
     3601 tgcgcgacaa cgggtacaag acctatctcg tcaccggggg cggccaggac ttcgtacgcg
     3661 ctttctccga gcaggtctac ggcatcccgc ccgagcaggt cgtgggctcg gccggcgcta
     3721 cgaagttcgg ctatgacggg aacggtcgcg cgacactcac caaggctccc aaggtggtcc
     3781 tgttcgacga caaagccgga aagccgaccg gcatccatct gatgatcggg cggcggccgg
     3841 tggcggcctt cggcaattcg acaggcgatc agcagatgct ggagtacacg caggcgggcg
     3901 gcgggcggcg gctcatgatg ctcgtgcaac acgac
//
LOCUS       DASSLD010000096         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_837706,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000096 DASSLD010000000
VERSION     DASSLD010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837706"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFK23_01740"
     CDS             <1..205
                     /locus_tag="VFK23_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7317834.1"
                     /translation="GFTGMGPLLRFALSQPVTNVVIRCDDAAQLEENVGFARSFAPMT
                     DVEKDELIRSISPYARELMFYKP"
     gene            complement(253..831)
                     /locus_tag="VFK23_01745"
     CDS             complement(253..831)
                     /locus_tag="VFK23_01745"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015852648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET7317835.1"
                     /translation="MAKIIVDKNLFCLPWTQTLLGSHVNGKVNFMALDWLTRVNYQPA
                     MIGICVNKGHATNGGIRASGEFSVNVPSDDMVTITDYCGITSAKSSDKSSLFEVFYGE
                     LKAAPLITDCPLNLECKLVQAVDLPTNTFFIGEIRNIYSEEKFMTDRKPDPKKMHPFL
                     LTMPDNRFWALGKQVGNAWTDGLKFKEKLKPS"
     gene            966..1235
                     /locus_tag="VFK23_01750"
     CDS             966..1235
                     /locus_tag="VFK23_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317836.1"
                     /translation="MQHSRTRTFEKIDKTRIDAILKGLIDNGSRVTGTNPWDVETRSH
                     GVRLRGEWNEEASMLTIAIVDADWYVPRKKIWENIESLMRIVEQR"
     gene            1417..1815
                     /locus_tag="VFK23_01755"
     CDS             1417..1815
                     /locus_tag="VFK23_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317837.1"
                     /translation="MISHLASALKTEDRMRIEEICRALALGVSSQLGVPPLRVRVFAV
                     RPSAAWGELHGLYELAAGRASAVISLWMRTAKHKRVVAFRAFLRTLLHEICHHLDYHL
                     YQLPDSFHTEGFYKRESSLFHQLVQEGDKG"
     gene            1981..2547
                     /locus_tag="VFK23_01760"
     CDS             1981..2547
                     /locus_tag="VFK23_01760"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET7317838.1"
                     /translation="MKKSLGAKTLAQPTPVWVVGSYDAQGKPNMMTIAWGGICCSQPP
                     CIAVSVRKVTYTYGNLVGRKAFTVNIPSTELIKEADYVGTVSGKSVDKFAMTGLTPVR
                     SELVDAPYVKEFPIVIECKLLHIVEIGQHTQFVGEIMDIKAEEAVLGEKGMADILKVK
                     PFVFDAGQRAYYGIGEQIGKAFSIGKRD"
     gene            complement(2634..>2948)
                     /locus_tag="VFK23_01765"
     CDS             complement(2634..>2948)
                     /locus_tag="VFK23_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET7317839.1"
                     /translation="PWGVKVTTVEVKNVDLPAEMQRAIAIQAQAERERRAKVIHAEGE
                     LQAASKLSEASDIISKNPTSLQLRYLQTLTEIASEKNSTIIFPLPIDLISAFQKLVAK
                     KD"
BASE COUNT          622 a          821 c          863 g          642 t
ORIGIN      
        1 gggattcacc ggcatggggc cgcttctgcg ctttgccctg tcgcagccgg tgacgaacgt
       61 ggtcatcagg tgtgacgacg cggcccagct cgaagaaaac gtcggcttcg cgcgctcgtt
      121 tgcgcccatg acggacgtcg aaaaagacga actgatccgg agcatctcac cttatgcgcg
      181 ggaattgatg ttttacaagc cttgaacatt cggaaaacgt cgtcattccc tctcgcctca
      241 aagcctgctt ttttacgaag gcttgagctt ctctttgaat ttcaaaccgt ccgtccaggc
      301 atttcccacc tgtttcccga gcgcccaaaa ccggttgtcc ggcatggtga gcaggaaggg
      361 atgcatcttc ttcgggtccg gcttcctgtc cgtcatgaac ttctcttccg aatagatgtt
      421 cctgatttcg ccgatgaaga aggtgttcgt gggaaggtcc acggcctgga ccagcttgca
      481 ttcgaggttc agggggcagt cggtgatcag gggggccgcc ttgagctctc cgtagaaaac
      541 ctcgaacaac gacgacttgt cggagctctt cgccgaagtg atgccgcagt aatccgttat
      601 cgtcaccatg tcgtccgagg gcacattcac gctgaattcg ccgctcgcgc ggatcccccc
      661 gttcgtagcg tgccccttgt tcacgcagat gccgatcatg gccggctggt aattcacccg
      721 cgtgagccag tcgagcgcca tgaaattgac cttgccgttc acgtggctgc ccaggagcgt
      781 ctgggtccag ggaaggcaga agaggttctt gtctacgatg atcttagcca tggcatgtcc
      841 tccttttgtc atgagtatag catgagggtt cgatggtcca gacggcttgc gggaaataaa
      901 ttgctataat tcacgcaggg gcggcaaacg ggaaaaaaac ctaaccggac cccggggaga
      961 accctgtgca gcattccaga acgcgaacct tcgaaaagat cgataaaacc agaatcgacg
     1021 ccatcctgaa gggcctgatc gacaacgggt cccgcgtgac cgggaccaac ccctgggacg
     1081 ttgagactcg ctctcacgga gtgcggctgc ggggagagtg gaatgaggaa gcgtcgatgc
     1141 tcactatcgc catcgtggac gccgactggt acgtcccccg gaagaaaata tgggagaaca
     1201 tagaatcact tatgcggatc gtggagcagc gttaggttgt gccgtctcac ttccccgcca
     1261 tggttttgca gtgtccgggc tgaccatgtc tttttgtaaa gatcggccat ggtttttccc
     1321 tattacaaca gactttcggc agctcgaaaa cgcatctacc gcagaagcga tgagattcat
     1381 tcagtcgctt tgcccgatgg ggaagcgcta cgaccgttga tatcgcacct ggcttcggca
     1441 ctcaagaccg aggaccggat gcgcatcgaa gagatatgcc gggccctggc cctgggggtg
     1501 tcaagccaat tgggcgtccc tccgctccgc gtcagggtgt ttgccgtccg gccgtcggcc
     1561 gcgtggggtg agctccatgg tttgtacgag ctggccgccg gcagggcgtc cgcggtcatt
     1621 tcactctgga tgcggacggc gaagcacaag cgtgtcgtgg cgttccgggc gttccttcgc
     1681 acgctcctcc acgagatctg ccaccacctg gattaccatt tgtatcaact gcccgactcg
     1741 ttccacaccg agggctttta caagcgggaa tcgagcctct ttcatcaact ggtgcaggaa
     1801 ggggacaaag gataatttcg cgcgattctt ggttacgagt tgcgggttac aagttgcgag
     1861 tttgaaaagc ctttaaaccg caacccgaaa cgcgtaacct ccaacccgtg acatttgcaa
     1921 catttcggcc ggaggccgtg ttatgctata gttactttaa ctcattccag ggaggttcgt
     1981 atgaaaaaat cactcggcgc caaaacactc gcccagccga cgccggtctg ggtagtcgga
     2041 agctatgatg cgcagggcaa accgaacatg atgacgatcg catggggcgg tatctgctgc
     2101 tcgcagccgc cgtgcatagc cgtatccgtg cgcaaagtga cctacacata cggaaatctc
     2161 gtcgggcgca aggcattcac cgtcaacatt ccttccacgg agctgataaa ggaggcggac
     2221 tatgtcggca cggtctcggg gaaaagcgtt gataagttcg cgatgaccgg gctcacgccc
     2281 gttcggagtg agctggtcga cgccccttat gtgaaggaat tccccatagt gatcgagtgc
     2341 aagctgcttc acatcgtcga gatcgggcag catacgcaat ttgtcggcga gatcatggac
     2401 atcaaggccg aagaagccgt gctcggggaa aaggggatgg cggacatcct gaaggtgaag
     2461 cccttcgttt tcgacgcggg gcagcgcgca tactacggca tcggagagca gatcggcaag
     2521 gctttttcga tcggcaagag agactaagaa gaccgcaaac atcgctcaca cacaaaaaag
     2581 ggcggcctcc aaaggccgcc ctttccgttg ctttcggaca gtccacttcg cctttaatcc
     2641 tttttcgcaa ccagcttctg gaacgccgag atcaggtcga tcggcagggg gaagatgatg
     2701 gtagagttct tctccgaggc gatctcggtc agggtctgga gataccggag ctggagcgag
     2761 gtcgggttct tgctgatgat gtcggaagcc tcggacagtt tgctggccgc ctggagttcg
     2821 ccttccgcat ggattacctt ggcccggcgt tcgcgctctg cctgggcctg gatggcgatg
     2881 gcgcgctgca tctcggccgg aaggtccaca tttttgacct ccacggtcgt caccttgacc
     2941 ccccaggg
//
LOCUS       DASSLD010000097         3834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_544268,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000097 DASSLD010000000
VERSION     DASSLD010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544268"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VFK23_01770"
     CDS             complement(<1..870)
                     /locus_tag="VFK23_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317840.1"
                     /translation="MKRWLAILTAAGLLLFPFAALGADYGSQQSQTQQAPPVAQQLVR
                     EGDFAIKLAAELDLGNPTDEATAEDILATAGVMPANGWLSDYPVTPQIIGQLQESIAK
                     AASEGKLPMNAEEATRGLYALAAQMDLPTPAAPGSSPSGGQQAPAAQTSPSVVNNYYY
                     NQGPPVVTYYPPPADYAYMYDWVPYPSWWFGFWFSGFFIARNFTTVVVVTRPVVVSGS
                     VVVTSRPVIVTNRIIDPVTRRVAVVDPVTRTGPGRTRPLTTLRSTDGRTFSNLSDLRS
                     GVNRPRTVMPGTSP"
     gene            complement(979..2739)
                     /gene="sppA"
                     /locus_tag="VFK23_01775"
     CDS             complement(979..2739)
                     /gene="sppA"
                     /locus_tag="VFK23_01775"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET7317841.1"
                     /translation="MSGNERIRGTGACSLIFFLFLFTGIAAGIYHWFVAGRVPNRVVL
                     EADFEQGLIEYVPEDASARFLLSKRLCVRDVVEALQKASGDDRVRGLIARVGESKMTL
                     AQVQEVRAAVISFRTAGKPALAYAETFGEAGPGNSSYYLATAFDEVYMQPSGDVGLTG
                     LMYEQPFFRGTLDKLGIIPRVDGRKEYKSFRYQFTERKYLPPHREAVEQVMKSRFGQM
                     VRGIAEARKLSEEEVRSLIDRGPFIGREAVDAKLVEGLAYRDQVYDRIKEKAGDKAEF
                     LTIGDYRKRADGPYEKGATVALIYGVGGIMRGKSGYNPATGEIIMGSDTLASAFRQAA
                     EDKDVKAILFRIDSPGGSVVASDTIWRETVNAKKAGKPVIVSMGSTAASGGYFVAMAA
                     DKIVAQPATATGSIGVLGGKVITTGFWSKLGVTWDEVHTSKNADAWTMTRDFTPQQRA
                     RFEEWLDRIYEDFTSKVGQGRNLSKEAVEKIAKGRVWTGEDAKRLGLVDELGGFPEAI
                     RLVRKAAKLSENAPIRLKLYPEATLLTRMSERFGLSTREADMESSLVRMLEDVQPVLK
                     TLESLGFSGRSEVLRAPDLD"
     gene            complement(2828..>3834)
                     /locus_tag="VFK23_01780"
     CDS             complement(2828..>3834)
                     /locus_tag="VFK23_01780"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7317842.1"
                     /translation="VAGEAQKLIPVPPPGTHSMDTKSIAVLLIEDDSNDALLIQRYLS
                     NTGKGPYEVNRVDRLRAGLDLLNRNNVDIVLLDLELPDGHGLSSFDQVHALAPHIPII
                     VMTGHDDDDVAVEAVQKGAQDFLTKGKVDGGLLQRAIRYAIERNKLLTDLKKAESARR
                     DAAEEMKRLNELLERQATTDSLTGFSNRLKFGESLKAEINRSRRYALPLSLIMFDIDH
                     FKGVNDTYGHLAGDKVLVGIADLVSKHVRVNDLFARWGGEEFMIMVMNSALDNARMSA
                     EKLRLLIEQHLFPGDIRVTCSFGVAQFLHDESEDRFIQRVDDALYLAKARGRNRLETA
                     "
BASE COUNT          734 a         1174 c         1101 g          825 t
ORIGIN      
        1 cggggaagtt cccggcatca cggtgcgggg cctgttcact ccactgcgca ggtccgacag
       61 gttgctaaac gtcctgccat ccgtggaccg gagagtcgtc agtggacggg tacgccccgg
      121 gcccgttcgc gtgaccggat cgaccacggc gacccttctc gtgacgggat cgatgatccg
      181 gttcgtgacg atgaccggcc tcgacgtcac tacaacggac ccgcttacga caacaggtct
      241 cgtcacgacg accactgttg tgaagttacg agcgatgaag aagcctgaga accagaagcc
      301 gaaccaccag ctcggatagg gcacccagtc gtacatgtat gcatagtccg cgggaggcgg
      361 ataataggtc actaccggag gcccctgatt gtaatagtag ttattgacca ccgacgggct
      421 ggtttgagcg gcaggcgcct gctgcccccc cgatggcgag cttccgggcg ccgccggggt
      481 cggaaggtcc atctgtgccg cgagagcata gagtccgcgc gtcgcctcct cggcattcat
      541 gggcagcttt ccctcggatg cggcctttgc gatggactcc tggagctgac cgatgatctg
      601 tggcgtcacc ggataatccg aaagccatcc gtttgcgggc atgacccctg ccgtggccag
      661 aatgtcctcg gcagtcgctt catcggtggg atttcccaaa tcgagttcag ccgccagctt
      721 gatcgcgaaa tctccttcgc gcaccagttg ctgggctacg ggaggagctt gttgcgtttg
      781 cgactgttgc gacccgtagt ccgctcccaa ggccgcgaag ggaaaaagga gcaagccggc
      841 tgccgtaagt atggcgagcc atcttttcat attcgtactc cttaacctac ttatattgta
      901 ctcccgggga tggggatgag gcaagggggg tggaataata ctactttatt accactaagg
      961 ttgcagttat gataatgttc agtcaagatc gggcgcgcga agaacttcgg aacgtccgga
     1021 gaaaccgagg gattccaggg ttttgagaac gggctgcacg tcctccagca tgcgcacgag
     1081 ggacgactcc atatccgcct cccgcgtcga gaggccgaag cgctcagaca tcctggtcag
     1141 aagtgtcgct tcagggtaga gcttcagccg gatcggtgca ttttcagaga gcttcgctgc
     1201 cttgcggacg agccggatag cctcgggaaa tcctcccagt tcgtccacga gtcccagcct
     1261 ctttgcatct tctcctgtcc agaccctgcc tttcgcgatt ttttccaccg cttcctttga
     1321 aaggtttctg ccctgaccaa ccttggacgt aaaatcctcg tagatacggt cgagccattc
     1381 ctcgaaccgg gcccgctgct gcggcgtgaa gtccctggtc atggtccatg cgtcagcgtt
     1441 tttgctcgtg tgcacctcgt cccaggtcac gccaagtttg ctccagaagc ccgttgtgat
     1501 caccttcccg cctaatacgc cgatagagcc ggtcgccgtc gcaggctggg ccacgatctt
     1561 gtcggcggcc atcgcgacga agtaaccacc cgaggctgcc gtgcttccca tggagacgat
     1621 gacgggcttg cccgctttct tggcgttcac ggtctcgcgc cagatggtgt ccgaagccac
     1681 gacagacccc ccgggactgt ctattcgaaa caagatagcc ttgacgtctt tgtcctcggc
     1741 cgcctgccgg aaagcggacg ccagcgtatc ggagcccata atgatctcgc cggttgcggg
     1801 attatagccg ctctttcccc gcatgattcc gcctacaccg tagatgaggg cgacggtcgc
     1861 gcctttttca tagggcccgt ctgcccgctt ccggtaatct ccgatcgtca ggaattcggc
     1921 cttgtcgccc gccttttcct tgatccggtc gtagacctgg tcgcggtacg cgagcccctc
     1981 cacgagtttc gcatcgaccg cctcacgccc gatgaacggc ccccggtcga tgagtgaacg
     2041 gacctcttcc tccgaaagct tgcgcgcctc ggcgatcccc ctgaccatct ggccgaaccg
     2101 ggatttcatc acctgctcga ccgcctcccg gtgaggcggc aggtatttgc gctccgtgaa
     2161 ctggtaccgg aaactcttgt attccttcct gccatccacg cgggggatga tgccgagttt
     2221 atccagcgtc ccccggaaaa aaggctgttc gtacatgaga cccgtgagac ccacatctcc
     2281 cgaaggctgc atatagacct catcgaaggc cgtcgcgaga tagtaggatg agttcccggg
     2341 gccggcctct ccgaaggtct cggcgtaggc tagggcgggt ttgcccgccg tacggaatga
     2401 gatgaccgcg gcgcggactt cctgcacctg cgccagcgtc atcttcgatt ctcccaccct
     2461 cgcaatgagt ccccggaccc ggtcgtctcc cgacgccttc tgaagtgctt cgacgacatc
     2521 gcgtacgcac agcctcttcg acaagaggaa acgcgcggag gcatcctcag gcacgtactc
     2581 gatcaacccc tgttcaaagt ccgcttcgag gacgacccga ttcggcacac ggcccgcgac
     2641 gaaccagtga taaatacccg ctgcgatccc cgtaaaaaga aacaggaaaa agatcaaaga
     2701 acaggcgcct gtgccgcgaa tgcgttcatt accggacatg gaaggctcct aacgcaaacg
     2761 agattctgga ttctgaattt ctatctagca tcaagcatcc agtatcgatt tttgttgccg
     2821 cccggtatca ggccgtctcg agccggtttc ggccccgtgc ctttgccagg tacagtgcgt
     2881 cgtccacgcg ctggatgaaa cggtcttcgc tctcatcatg caggaactgg gctaccccga
     2941 aactgcacgt cacgcgaata tccccgggga aaaggtgttg ttcgatcaag agccgcaatt
     3001 tttcggcgga catccgggca ttgtccagcg cggagttcat caccatgatc atgaactctt
     3061 caccgcccca ccgcgcgaac aggtcgttga ccctgacgtg tttcgagacg aggtcggcga
     3121 tcccgaccag gactttgtcg ccggcaagat ggccgtacgt gtcgttgaca cccttgaagt
     3181 ggtcgatgtc gaacatgata agcgagagcg gcagtgcata ccgcctcgac cggttgattt
     3241 ccgccttcag cgattccccg aacttgagcc ggttggagaa gcccgtgagc gagtcggttg
     3301 tcgcctggcg ctccagaagc tcattgagcc gcttcatctc ctcggccgcg tcccgccggg
     3361 cgctttccgc ctttttgagg tcagtgagca gtttattgcg ctcgattgcg tagcggatcg
     3421 cgcgctgcag caggcccccg tccacctttc ccttggtgag aaaatcctgc gcaccttttt
     3481 gaacggcctc gacggctaca tcgtcgtcgt catggccggt cattacgatg atggggatgt
     3541 ggggggctag cgcatgcact tgatcaaagg aactgaggcc gtgcccatct ggcagttcga
     3601 ggtccaggag cacgatgtcg acgttattcc ggttcaggag gtccaggcct gcccggagcc
     3661 ggtcgacacg gttgacttcg taggggcctt ttcctgtatt ggataaatac cgctgaataa
     3721 ggagggcatc gttggaatcg tcttcgatga gcaggacagc gattgacttg gtatccatgg
     3781 aatgtgttcc cggcggtggc acgggaatca gcttctgcgc ttcgccagcc actc
//
LOCUS       DASSLD010000098         3694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_576527,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000098 DASSLD010000000
VERSION     DASSLD010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576527"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFK23_01785"
     CDS             complement(<1..185)
                     /locus_tag="VFK23_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317843.1"
                     /translation="MYIVVEHTITNPDVYFGLASRVAEAPAGINAVQFFPSMSKDRAV
                     CLWEAKSVDALKGFLDP"
     gene            589..1077
                     /locus_tag="VFK23_01790"
     CDS             589..1077
                     /locus_tag="VFK23_01790"
                     /inference="COORDINATES: protein motif:HMM:NF025252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET7317844.1"
                     /translation="METTEREKLARLIRGGRWAAIATYGDGVPLASMVAYACEPGFAG
                     FLLHLSRLSAHTRNLLASPSVSIAISEPDTAQIRDPQMLARVSIQGEARPLQKDLKGY
                     SKAKALYEKRFPDSSPLFGFGDFLLFRLVPREARFVAGFARAYTVDLEELKEAAAVRF
                     SP"
     gene            1309..1611
                     /locus_tag="VFK23_01795"
     CDS             1309..1611
                     /locus_tag="VFK23_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317845.1"
                     /translation="MTRRFWVPATALFLSSIMAMVGFVPQDAMAGIVALSQSIGASIV
                     QEEGPAKVHSATDFAPHPQRNVSARYWMRGIVLLLIILLMLWLIYRTFTGWKPMIS"
     gene            complement(1839..2033)
                     /locus_tag="VFK23_01800"
     CDS             complement(1839..2033)
                     /locus_tag="VFK23_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317846.1"
                     /translation="MKTTMIYVDVGKPHIREQVEKLDRIVVPRPQNRMKALAALTSPP
                     WSVHPNLRGRILPEFPSNYR"
     gene            complement(2211..2750)
                     /locus_tag="VFK23_01805"
     CDS             complement(2211..2750)
                     /locus_tag="VFK23_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HET7317847.1"
                     /translation="MNKSILKGTLLGGLSILIFAVLAVGGYKVLHRPKVAEVVAVKEV
                     TETAMTPREECRDIQVRQRAPVNDSNRIAGTVLGGITGGIIGHQFGSGTGNALSTIGG
                     AAGGAYVGNKVQESMQNNDVSTTTRRSCKTVYDKTQKLVGYDVTYRLNDKEGVVRTSF
                     RPGPTLPVEKGQVVTTRPS"
     gene            complement(2864..3325)
                     /locus_tag="VFK23_01810"
     CDS             complement(2864..3325)
                     /locus_tag="VFK23_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317848.1"
                     /translation="MKTKIHPALSLFALIGFVATTVPVHAGTNVNVNLGIPVPVVVAP
                     QPVYIEQPPEMVFIPQSNVYFAFAPGVSVDIFFYDNRWWNRRGDRWYRGNSYNGPWVA
                     VGPRYVPVPVYRMPADYRTFYVHEKHIPYGQWKKNHGEHGRHGNQHGHHDD"
BASE COUNT          775 a         1055 c         1006 g          858 t
ORIGIN      
        1 aagggatcca agaagccctt caaagcgtct accgacttgg cttcccaaag acagaccgcc
       61 cgatctttgc tcatactcgg gaaaaactgg actgcattaa ttcccgcagg tgcttcggcg
      121 actcttgagg caagcccaaa ataaacatcc ggattagtaa tcgtgtgctc gacaacaata
      181 tacatatcaa cctcctttca ggttgcatga ctgcaacacg ttaaatctgt tgagtgttgg
      241 tcgtgcccca cttcctcgac acgtgctacc cctcctttct tgacaatcac actgggacga
      301 ggggctatac gattccgaaa gaaatcgcgt agatcaactc ccaatgagtg tcagacttcc
      361 tcatttatat ttgattcgtt cgagccatcc aagatgcgtc ccgagatggt tccaactttt
      421 tcaggaaagc cggacgcact gcaaaccccg tgcaaacaac tgccttttac taggtgaaaa
      481 tggggtttat cttggatgct gttgacgggg gaaacctctt atgcggttgg ataaccttac
      541 ataacatagg gtgagtcacg ataccgcatc ccgaaggagg ggaatggcat ggagacaacg
      601 gaacgagaga aactagcgcg cctgatccgt ggagggcgtt gggctgccat tgcgacgtac
      661 ggggatgggg tgccgctggc atcgatggtg gcctatgcct gcgagccggg gttcgccggg
      721 ttcctcttgc atctgagccg tctctcagcc cacacgagga atctcctggc gtccccatct
      781 gtctcgatcg cgatcagcga gccggacacg gcccaaattc gcgatcccca aatgctggcg
      841 cgggtcagca tccaggggga ggcacggccc ctccagaagg acctgaaggg gtactcgaag
      901 gcgaaagcac tctacgaaaa acggttcccc gattcgtccc ccctcttcgg attcggagat
      961 ttcctcctgt tccgcctcgt cccccgggag gcccgctttg ttgcggggtt tgccagggcc
     1021 tacaccgtcg acttggagga actgaaagaa gccgcggccg tccgtttcag tccttaatat
     1081 tgtgaatacc ctcggagcaa ttgacctgcg ccccgatttt aggccaactt gacgagggta
     1141 atttcaccga agtctgcacg gactgtaaac cccgtgcaaa caaccggctt tttgggggtg
     1201 aaatagggat aatacagggg cattgtcgac gggggaaatc cccttgtgca gttggaaaag
     1261 tttcaggggg gaggtcacaa ggtttctgac gcttctgcgg gaggatgaat gacgcggaga
     1321 ttctgggttc cggcaaccgc cctctttctc tcttccatca tggcgatggt agggttcgtt
     1381 ccgcaggacg ccatggcggg aatcgtcgcc ctgtcgcaat ccataggcgc atccatcgtc
     1441 caggaggaag gccccgcgaa ggtccactcc gcgacagact tcgcacccca tccgcaacgg
     1501 aacgtatccg cccggtattg gatgcgaggc atcgtgctcc tcctcatcat ccttctcatg
     1561 ctctggctga tctaccggac cttcaccggg tggaagccga tgatttcgta gtttgcgtcg
     1621 gaaacgctaa cggtttccaa taggcgaaag ttgccgccat tgagtgaccg gtccccggaa
     1681 aatgcgatca actgtacgag gatatttccg tcgaagtccg cgcggaccgc aaaccccttg
     1741 caaacaagtg ccattttcgg ggtctggaag ggggttttag cgttgcctgt tgacgcggga
     1801 aaccccttgt atcggtgggg tggctaacgg agagcggatc acctgtagtt cgatggaaac
     1861 tccgggagga ttcttcccct aagattgggg tggaccgacc atggggggct ggtcaaggca
     1921 gcaagcgcct tcatcctgtt ctgcggtcga ggtaccacga tcctgtccag cttctccacc
     1981 tgctccctga tgtgcggttt gcccacgtcc acgtagatca tcgtcgtctt catgtcagcg
     2041 tgccccatcg ccttcatgat ggcgaatttt ttaaacttcc ggctggacca aggttgggtg
     2101 aggttcaaaa gacatagccc gcggtcaggt cgaatcccga ccacgggctt tggatggtgt
     2161 tggctccacg cacagattat aatccggtcc cgacacccac cggtcttcgt ttaactcggc
     2221 cgagttgtca cgacttggcc tttttccacg gggagtgtgg ggcctggacg gaaagatgtc
     2281 cttacaaccc cttccttgtc gttcagccga tatgtaacgt cgtatccgac caatttctgc
     2341 gttttgtcat agacggtttt acaacttcgc cttgtcgttg tggacacgtc gttgttctgc
     2401 atgctttctt gcaccttgtt cccgacgtac gctccgccag cggccccgcc aatcgtggag
     2461 agcgcattgc ctgtgccgct gccgaattgg tggccgataa tgccgcctgt aatcccgccg
     2521 agaacggtac cggcgatacg gttggagtca ttcaccggag cccgctgtcg cacctggata
     2581 tcgcgacact cttcgcgcgg agtcatggcg gtctctgtga cctcctttac tgcgacaact
     2641 tccgcaacct tcggacgatg cagtaccttg taccccccaa cggcgagcac cgcgaagatc
     2701 aatatagaca aaccgccaag caaggtacct ttgagaatgg atttattcat ggcatctcct
     2761 tatcaaagaa ggaccgcttc ctggtccagc ccgaggatta acaattgacc tgcagccctc
     2821 cgatcgatcc ggcgtctgct cggacttcag ccgatttgcc ctgctaatca tcgtgatgac
     2881 cgtgctgatt gccgtgccgg ccgtgttccc cgtggttctt cttccactgg ccgtacggga
     2941 tgtgtttctc gtggacgtag aaagtgcggt agtccgcggg cattcggtag acggggacgg
     3001 ggacataccg cggccccacg gccacccacg gcccgttgta gctgttgccc cggtaccacc
     3061 gatccccccg gcgattccac caacggttgt cgtagaagaa gatgtccacg cttacacccg
     3121 gagcgaacgc gaagtagacg ttcgactggg ggatgaaaac catctctgga ggctgctcaa
     3181 tatacaccgg ctgaggagcg acgaccaccg ggaccgggat ccccaagttg acattgacgt
     3241 tggtcccggc atgcactggg actgtggtcg cgacgaatcc aataagagcg aatagagaca
     3301 gagccggatg aattttggtt ttcataccaa tccctccttt tccgccttca ggcctacgta
     3361 cggtttcctc accggggatc ctttccactt cccccatcaa aaagctttta atctccccct
     3421 cacaatccac ctcccgcctc agatcgagtt acgagtatct atgtgggttg attaaactta
     3481 gattcgaatg accctcccct gggattcggg attcggaaca cataaaaatc attgtatttc
     3541 aacgagttcc gggaaacagg ttggtcatgg ggccgaatga tccatctgta gggcctaccc
     3601 tagggcctgc cgcgaggggg agcccggatg tgccctcggg ttcgatctat tgcgggatgg
     3661 ttactttatg tgcgtgatct actaggtgta gcac
//
LOCUS       DASSLD010000099         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_510911,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000099 DASSLD010000000
VERSION     DASSLD010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510911"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VFK23_01815"
     CDS             <1..613
                     /locus_tag="VFK23_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7317849.1"
                     /translation="MKQQLPFITLMLLATVRFLSNFWWALLLAIAGGVYWFRRYLRTQ
                     AGREAFDARILRMPVFGSLIRMIAVSRFTRTLGTLLEGGVPTLTALDIVKSVVGNTVL
                     ANAIQQARENVREGEPIADPLKRSGLFPPVVVQMVAVGEKSGELEKMLLKISDSFDRT
                     VETRITGLMALMEPVIILAMGLIIGFIVIAIMLPMLEMSSGVR"
     gene            713..1147
                     /gene="gspG"
                     /locus_tag="VFK23_01820"
     CDS             713..1147
                     /gene="gspG"
                     /locus_tag="VFK23_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HET7317850.1"
                     /translation="MKRQQGFTLIEIMVVIIILGLLAGLVLPRFLGQEEKAKVEVAKT
                     QIRSLEGALDAYKLDNGFYPTTDQGLDALIKKPETGRIPEKWREGGYLKPARIPKDPW
                     GKDYVYLSPGSEGREYEIVSYGADGEPGGEGNKADIESWKMQ"
     gene            1179..1742
                     /locus_tag="VFK23_01825"
     CDS             1179..1742
                     /locus_tag="VFK23_01825"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET7317851.1"
                     /translation="MKWKRIDACETVLTPNSEHRTLNSRGFTLIELTVVIVVLGVMLS
                     LIIPRLGELGEANLKRSARHLTGMIRFLHDEAQAKKDTYRLRFDITEGRYWVDVLTFN
                     LSDKTAEYKRFSSEMATEGSLSGRTTFRDVKVASHPDEPYIEFSSNGWVERALIHLRD
                     GDGRDFTLTVNPLTGNTAFDEGYIEER"
     gene            1769..2191
                     /locus_tag="VFK23_01830"
     CDS             1769..2191
                     /locus_tag="VFK23_01830"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7317852.1"
                     /translation="MADETGHADSEWKTELPHSAFRTPHSKGFTLLEVMVAVSIMAMV
                     LVSLLGLKNRSMQDVTVADHITTATILAKRTMVDVMFSKPSKPDEGEGTFPEEEYKDY
                     TWKKTISATPFVQIMEVRVAVLWKDGERQEMVELVSYE"
     gene            2256..>2457
                     /locus_tag="VFK23_01835"
     CDS             2256..>2457
                     /locus_tag="VFK23_01835"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7317853.1"
                     /translation="MPRSSPFSSIPHPAFRIPNSQGFTLLEVLIAVAIMSAIVTVIYA
                     SFSTASRNVQQAEEIRDSADLAR"
BASE COUNT          541 a          673 c          771 g          472 t
ORIGIN      
        1 catgaagcag cagctgccgt tcatcacgct catgctcctc gccacggtgc ggttcctctc
       61 gaacttctgg tgggcgctcc tgctggccat cgccgggggc gtctactggt tccggcgcta
      121 tctgcggacg caggcgggca gggaggcctt cgacgcgcgc atcctcagga tgcctgtctt
      181 cggaagcctc atccgcatga tcgccgtgtc gcggttcacg cgcacattgg gaacgctgct
      241 cgagggcggc gtgccgacgc tcaccgccct cgacatcgtg aagagcgttg tgggcaacac
      301 ggtcctcgcg aacgccatcc agcaggcccg ggagaacgtg cgggaaggcg aacccatcgc
      361 cgatcccctg aagagaagcg gcctgttccc gccggtggtg gtgcagatgg tcgccgtggg
      421 cgagaagagc ggggagctcg agaagatgct cctgaagatc tccgattcct tcgaccgcac
      481 cgtcgagacg cgcatcacgg gcctcatggc gctcatggag ccggtgatca tccttgccat
      541 gggtcttatc atcgggttca tcgtgatcgc catcatgctt ccaatgctcg agatgagttc
      601 gggagttcga tgaagtgttg ttgagttcgg agtgaggacc ggaatccaac gtccggggat
      661 gcctgcggac cagggattgg ataatgagat cgaacgtaaa aggagattgt tcatgaaacg
      721 acaacaaggc tttaccctta tcgaaatcat ggtcgtcatc atcattctgg gcttgctggc
      781 gggcctggtg ctcccccggt ttctcggaca ggaggagaag gccaaggtcg aggtggcgaa
      841 gacgcagatc cgctccctcg agggggcgct tgacgcctac aagctcgaca acgggttcta
      901 ccccacgacc gaccagggac tggacgccct gatcaaaaag ccggaaaccg ggcgcatccc
      961 ggaaaagtgg cgcgagggcg ggtatctcaa gcccgcgcgg atcccgaagg acccctgggg
     1021 caaggactac gtctacctct ctccggggag cgaaggccgg gagtacgaga tagtctccta
     1081 cggtgcggac ggcgagccag gcggcgaggg caacaaagcg gacatcgaga gctggaagat
     1141 gcagtgaagt tcggagtcgg gagttcggag tggggagtat gaagtggaaa aggattgacg
     1201 cttgcgaaac ggtgctgact ccgaactctg aacaccgaac tctgaactca aggggtttca
     1261 ccctcatcga gctcacggtc gtgatcgtgg tcctgggggt catgctttcc ctgatcattc
     1321 cgcggctcgg agagctgggc gaagcgaacc tgaagaggag cgcgaggcac ctgaccggca
     1381 tgatccggtt cctgcatgat gaggctcagg cgaagaaaga cacgtaccgg ctgcgcttcg
     1441 atataactga gggacgctac tgggtcgatg tgttgacctt taacctgagc gacaagactg
     1501 ccgaatacaa acggttctca tcggaaatgg cgaccgaggg aagtctttca ggccggacga
     1561 cgtttcgcga tgtcaaggtt gccagccatc ctgacgagcc gtatatcgaa ttttcgtcca
     1621 atgggtgggt agagcgcgct ctgatccacc tgcgcgacgg cgacgggcgg gacttcacac
     1681 tcacggtaaa tccgctcacc ggcaataccg catttgatga aggatacatc gaggaacgat
     1741 gacgaataat tcggaattcg gaattcggat ggccgatgaa acgggccacg ctgattcgga
     1801 atggaaaact gaactcccgc attccgcatt ccgcactcca cattcgaaag gcttcaccct
     1861 cctcgaggtc atggtcgcgg tatccatcat ggccatggtc ctggtgtcat tactgggcct
     1921 gaagaaccgg agcatgcagg acgtgacggt ggctgatcac ataacgacgg cgacaatact
     1981 tgccaagcgt accatggtgg acgtgatgtt ttccaagccg agcaaaccgg atgaaggaga
     2041 agggacattc cccgaggagg aatacaagga ctatacctgg aaaaagacca tatccgccac
     2101 tcccttcgtc cagatcatgg aggtgcgcgt cgcggtgctc tggaaagatg gcgagcggca
     2161 ggagatggtt gagctggtga gctatgagta acaatgcgga atgcggaatt cggaattcgg
     2221 aattaaacag aggaaaaatt caggacacgg gtcgggtgcc gcgaagttcg cctttttctt
     2281 ccattccgca tcccgcattc cgaattccga attcgcaggg cttcaccctg ctcgaagtcc
     2341 tgatcgccgt ggcgatcatg tccgccatcg tcacggtcat ttatgcgagc ttctcgaccg
     2401 cgagccggaa cgtgcagcag gccgaggaga tacgggattc ggccgatctc gcccgcg
//
LOCUS       DASSLD010000100         3456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1620254, whole genome shotgun sequence.
ACCESSION   DASSLD010000100 DASSLD010000000
VERSION     DASSLD010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620254"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFK23_01840"
     CDS             complement(<1..264)
                     /locus_tag="VFK23_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HET7317854.1"
                     /translation="MVTALVLLKVARDKVNSVAETLADMDGITEVYSVAGRYDLTVII
                     RVKDNDQLAELVTNHMLKVEGIQKSETLIAFRVYSRHDLESMFS"
     gene            complement(299..1234)
                     /locus_tag="VFK23_01845"
     CDS             complement(299..1234)
                     /locus_tag="VFK23_01845"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7317855.1"
                     /translation="MKTGPAYIQFYPTLRCNKACDFCFNRSMPFAEDMALSDFLKMLE
                     VLERTPVETIDIIGGEPTLHPDIVSFIAGARDRGFSVNISTNGTNPAVLGEIMKIGKA
                     VTVGISINDRETLTQVRGFIQRHKPVVKTVYSPGMDRGMIGEILSLGPERFYLIYRDA
                     LDARELDATVPFHEFKSGIERAFDHRQVGMVYCAGFLPDSERYPELADVRCPSGTTKL
                     GVMPDGSVYPCNLLFGRKDFLLGNILTDPFTGIWHHGRLDFFRTASGNACPDKSCTLH
                     SKCHGGCPAHGLFITGDLAAPDPRCVAAARPESLL"
     gene            complement(1239..2066)
                     /locus_tag="VFK23_01850"
     CDS             complement(1239..2066)
                     /locus_tag="VFK23_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET7317856.1"
                     /translation="MSKSLTPAQVRAFRKKIYDYYDNEGRDLPWRKKVNPYRVLVSEI
                     MLQQTQVERVIEKYRDFLAAFPNFSALAEAPLYKLLTVWSGMGYNRRALALRALARKI
                     VDEHGGRFPRDREKLIALPGIGEYTAGAVMAFAFDKPVVFIDTNIRRVFIHEFFHDRQ
                     NIHDDEIVPLVERTMDIGNPRKWYNALMDYGTMLKEKHGNPNRKSVHYMRQSPFDGSN
                     RQVRGRILKTLVAGSPLSAAEIVKKSGMDRERVIKNLADLEREGFIRKQGRNYLIPH"
     gene            complement(2148..2306)
                     /locus_tag="VFK23_01855"
     CDS             complement(2148..2306)
                     /locus_tag="VFK23_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317857.1"
                     /translation="MNIFLTIAVVLAAVYGFAAIGYYYAFKNWSPMCGSRKSSSASAK
                     TIQWLLRK"
     gene            complement(2405..3043)
                     /locus_tag="VFK23_01860"
     CDS             complement(2405..3043)
                     /locus_tag="VFK23_01860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015848980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7317858.1"
                     /translation="MPGIQTFDKHVNEYEQWFVDHPLAYVSELHAVRDLLPKRGSGVE
                     IGVGTGRFAAPLGIKRGIEPSKPMAELARRKGIEVAFGVAEKLPFRDGEFDFALMVTT
                     VCFLDDPDLAFHEAYRVLRYGSPLIIGFIDRESPLGRGYLKRRDKSVFYKDATFYSVG
                     EIVEHLAKAGFVDFSFRQTLFGNLGDMEKADPVKEGHGEGAFVAVRGVKGPG"
     gene            complement(3049..>3456)
                     /locus_tag="VFK23_01865"
     CDS             complement(3049..>3456)
                     /locus_tag="VFK23_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET7317859.1"
                     /translation="KALEALAEIAAGARPIHLPDTQEYVEWTNPGYHEHIIPKLHGGH
                     FSVQAFLDLHGCTVPEAEEELETFFQESFRQGFRCVKIIHGRGLRSVRGPRIKDAVVR
                     RLSGHFRKDIIAFVTARQCDGGLGALYVLLRKK"
BASE COUNT          727 a          999 c          951 g          779 t
ORIGIN      
        1 ggaaaacatg ctctccagat cgtggcggga atagacgcgg aaggcgatga gcgtttccga
       61 cttctggata ccttccacct tcagcatgtg gttggtaacg agttcggcga gctggtcgtt
      121 gtccttgacc cggatgatca ccgtcaaatc gtatcttccg gcgacggaat acacttcggt
      181 gatgccgtcc atgtcggcga gcgtttccgc cacgctgttc accttgtcac gcgcaacttt
      241 cagcagcact aatgcagtaa ccatgcggac ctcctgaaca atcgatttta cttcatgatt
      301 atagcagact ttctggacgg gccgcggcaa cgcaccgcgg gtcaggggcg gcaagatcgc
      361 cggtgatgaa gaggccgtgg gcagggcagc cgccgtggca ttttgaatgg agggtgcagg
      421 acttatcggg gcatgcgttt ccggaagcgg tgcggaaaaa gtcgagccgt ccatggtgcc
      481 agatgcccgt aaagggatcg gtgaggatgt tccccagcag gaaatccttc ctcccgaaga
      541 gaaggttgca ggggtagacc gagccgtcag gcatcacgcc cagttttgtg gtccccgaag
      601 gacaccgcac atctgcgagt tccgggtagc gttcactgtc cgggaggaaa cccgcgcagt
      661 aaaccatgcc cacttgccga tggtcgaagg cccgctcaat gccggactta aactcgtgaa
      721 acggaacggt cgcgtcaagt tcccgcgcgt caagcgcatc gcggtaaatg agatagaatc
      781 tttccggccc gagggagagt atctccccga tcatgccgcg gtccataccg ggactgtaga
      841 ccgtcttgac cacgggttta tgcctttgaa tgaacccccg aacctgtgtg agcgtctccc
      901 ggtcattgat cgagatgcca accgtcacgg ccttgcctat cttcatgatc tcgccaagca
      961 ccgcgggatt ggtcccgttc gtgctgatgt tcacggaaaa cccccgatcc cgagccccgg
     1021 cgatgaacga cacgatatcg ggatgaagcg tcggctcgcc gccgatgatg tcgattgtct
     1081 cgaccggtgt ccgctcgagg acttccagca tcttcaggaa gtcggaaagg gccatgtcct
     1141 ccgcaaaggg catggacctg ttgaagcaga aatcgcatgc cttattacag cgaagtgtgg
     1201 gatagaactg tatgtaagcg ggacctgttt tcatgtgttc aatgcggaat gaggtaattt
     1261 cttccttgct tccgaataaa tccttcccgc tccaagtcgg cgaggttctt tataacgcgc
     1321 tcccggtcca tccctgactt tttcacgatc tcggccgcgc tcaggggtga cccggccacg
     1381 agggtcttca atatccttcc ccgcacctga cgattcgaac cgtcaaaggg gctctggcgc
     1441 atatagtgaa cgcttttgcg gttgggattg ccgtgcttct ccttcagcat cgtcccgtaa
     1501 tccataagag cattgtacca tttccgggga tttccgatgt ccatcgtccg ctccacgaga
     1561 ggaactatct catcgtcatg gatgttctgt ctgtcatgaa agaattcgtg gataaacacc
     1621 cgccggatgt tcgtatcgat gaaaacaacg ggtttatcaa atgcgaaggc catcacggcg
     1681 cctgccgtgt attcgccgat ccccggcagg gcaatcagtt tttcacggtc ccggggaaac
     1741 ctgccgccgt gttcgtccac gatttttcga gcaagcgccc tgagtgcgag agcacgccga
     1801 ttgtagccca ttccggacca aaccgtgagc agtttgtaaa gcggcgcctc tgcgagcgct
     1861 gaaaaattcg gaaaggccgc aagaaagtca cgatactttt cgataacgcg ctccacctgc
     1921 gtctgctgga gcatgatctc ggagaccaga acacgataag gattgacttt ttttctccag
     1981 gggaggtccc ggccttcgtt atcgtagtag tcgtagattt ttttccggaa cgcgcggacc
     2041 tgagctggtg taagagattt ggacatttta agttggcgtg ttgttcgaat gcaagtgtca
     2101 gaaacgggga cttacaaaat ttcccgttcg atctgccgtc cttcttccta tttgcgcagg
     2161 agccactgga tagtcttcgc tgacgccgac gacgatttgc gcgacccgca catgggtgac
     2221 cagttcttga acgcgtaata gtatccgatg gcggcgaagc cgtaaacagc cgccagtacc
     2281 acggcgatag tgagaaaaat attcattgtt tctatccttc ccgacatttt ctaccagata
     2341 tggtcagtga catgaatttt tatggcacgg ggagtgtaac agaaatcggc tctcatgtaa
     2401 attgtcatcc cggtcctttt acccccctca ccgcgacgaa agccccttct ccatgcccct
     2461 ccttcacggg atcggccttt tccatatcgc cgagatttcc gaagagcgtc tggcgaaagg
     2521 aaaagtcaac gaaacccgcc ttggcgagat gctccacgat ctcgcctacg gagtaaaagg
     2581 tcgcgtcctt gtaaaagacg ctcttgtccc tgcgcttcag gtaccccctg ccgagcggac
     2641 tctcccgatc gatgaacccg atgatgagcg ggctcccgta cctgagcacc ctgtaggcct
     2701 catgaaacgc aaggtccggg tcgtccagaa agcagaccgt ggtcaccatg agcgcaaaat
     2761 cgaactctcc gtccctgaat ggaagcttct cggcgacgcc gaaggcgacc tcgattccct
     2821 tccttcgggc gagttccgcc atcggctttg acggctctat tcctcgtttg atcccgagag
     2881 gcgccgcaaa cctgccggtc ccgactccga tctcgactcc gctccctctt ttcggcagga
     2941 ggtcgcggac cgcgtgaagc tcggacacgt acgcgagcgg atggtcgacg aaccactgct
     3001 cgtactcgtt cacatgcttg tcaaaggttt gtatgcccgg catgacggct atttctttct
     3061 cagcaagaca tacagcgccc ccaggccgcc gtcgcactga cgcgcggtca cgaaggcgat
     3121 gatgtccttg cggaagtgac cggagagacg cctgaccacc gcgtccttga tcctcggccc
     3181 cctgacggag cggagtcccc tgccgtgaat gatcttgaca cagcgaaaac cctgcctgaa
     3241 ggactcctga aaaaacgttt cgagttcctc ctccgcctcc ggaacggtgc agccgtggag
     3301 gtcgaggaat gcctgcacgg agaagtgccc tccgtgcagt ttcgggatga tgtgttcatg
     3361 atacccgggg ttcgtccatt cgacgtactc ctgcgtatcg ggcagatgaa tcggccgcgc
     3421 tcccgcggca atttcggcga gcgcttcgag cgcttt
//
LOCUS       DASSLD010000101         8670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_217847,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000101 DASSLD010000000
VERSION     DASSLD010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217847"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..594)
                     /locus_tag="VFK23_01870"
     CDS             complement(118..594)
                     /locus_tag="VFK23_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HET7317860.1"
                     /translation="MNRYSIDEIMEMAVQTEVLGTQFYTGMAEKFKKDDGLVKLFTDL
                     ARKEKEHEKTFKGIRDAVTKNGAEPVQWEEVSNYMRAFVESEFFLGKGKSLPSLDHIK
                     SVKDAVRFAIGFEKETLLYFLELRTIVKDKAAVDEVINEEKAHIRWLDTFRAGMEK"
     gene            complement(819..1760)
                     /locus_tag="VFK23_01875"
     CDS             complement(819..1760)
                     /locus_tag="VFK23_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET7317861.1"
                     /translation="MDNKITIVTLGGNAIIRRGEKGTVEEQFAHADACMAEVAKMLAA
                     GRRVIITHGNGPIVGNIVIRNECARNTIPPMPLFMNDADSEGGIGFMIQQTLYNSLRK
                     IGGKRDVATIITQVVVDENDPAFLNPTKPIGPYYREDEAEALRSEKGWTMVKGTGGYR
                     RAVPSPRPVRIIEKDVIKRLALEGIVVIAAGGGGVPVLETPEGLLRGIDAVIDKDLST
                     SALAREIGAEGIIILTEVDRVYLNYGATGEKGLDRASLVEMKRYFAEGHFPPGSMGPK
                     IEAAIEFLEAGGREVVITRPELMGAALAGQAGTRIGG"
     gene            complement(1872..3227)
                     /locus_tag="VFK23_01880"
     CDS             complement(1872..3227)
                     /locus_tag="VFK23_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HET7317862.1"
                     /translation="MKRQRVIIMGAAGRDFHNFNMVYRGDPDFEVVAFTAAQIPMIGG
                     RLYPPGLSGPLYPQGIPIRPEEELPELIGKFRVDQVAFSYSDVSHEYVMHKASLCMAL
                     GCDFTLLGPKKTMIESSRPVLSVCAVRTGAGKSGITKYIAGVLMGMGMRPVAIRHPMP
                     YSDLIKNRVQRYGSMSDVRACGCTIEEREEYEPLAGAGVVVYAGVDYEAIVEAAEKEA
                     DVLLWDGGNNDLPFLRPDLEVVVLDPLRPGHEITFHPGEANLRRAHIAVINKVNTAGR
                     DAVAKVEDNIRAINGSARIIHTASRVRADNPGAIRGRRVLVVEDGPTLTHGGMAYGAG
                     VIAARDYGAAELVDPRPFAIGSIRETFEEYPHIGNLVPAMGYSDRQMRELKETIESTP
                     CDLVLIATPVDLKALLELSKETVRVTYDIEEMEGAPLKKEIENWLLQGARSKIQAPNP
                     K"
     gene            complement(3422..4672)
                     /locus_tag="VFK23_01885"
     CDS             complement(3422..4672)
                     /locus_tag="VFK23_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7317863.1"
                     /translation="MVTNQPQGPIVAYRRYWLPELVVLTAAALGAIILFAATDLDITT
                     MRPFYHPESADPWPVANHPVWALFYRSAPWVTASLAVAGVSLSIIGVLRERSKRLRLY
                     GLFILLCVAVGPGLIINVVLKDHWGRPRPRQITEFGGRLEYTQPFVPTVSYGKSFPCG
                     HCSVGYLYAVGWWVWRRRRPRLAVASLVSGLILGTLLGIGRMAGGAHFLSDAVWSALL
                     AWGIAHGLYYYALRVPAREDGRAALYPLIVGSRRMKTAVIVAAIFVGLLIIGGGMLAN
                     PYYEELRPCKPPSHLSAPRNLEVLVDQLDVELYLVADTKGEIACSGVVHGFGLPGNEI
                     DSSAEFTDGPVPALRYRVSLKGWFTDIDGVGQFRVPVRDIKTIRVRARRGDITVIDET
                     GGLLTAKGGPAIDLHTTRGRVQYR"
     gene            4860..6095
                     /gene="pfp"
                     /locus_tag="VFK23_01890"
     CDS             4860..6095
                     /gene="pfp"
                     /locus_tag="VFK23_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HET7317864.1"
                     /translation="MGDKLAVVVGGGPAPGINGVISAVTYEAAKQGLEVVGVHDGFKW
                     LSKGDTGHTTNLCKSDISRIHMRGGSVIGTSRENPTKSPEKMANVIKALTELGIKYLV
                     TIGGDDTAFTATRVEQEAGGRISVCHVPKTIDNDLPLPHGVPTFGFETARSLGTQLVE
                     NLMEDARTAARWYFVIAMGRSAGHLALGMGMGAGATLTLIPEELGSKISLTKVVSILE
                     GAIIKRLSLGKGHGVAVIAEGVATRFDPEELTFLKDVPRDDHGNIRLAEIPIGDMLKQ
                     RVMTDLASIGLKTTVIAKDIGYELRCHPPISYDREYTRYLGFSAVKFLVSGGSGAMIM
                     LKEGVSLPIKFTDLLDPKTGKTRIRTVDINSDDYHIARRYMIRLEKEDMEGEGIKKVA
                     AAAKMAPEDFKKRFGVAVG"
     gene            complement(6208..6489)
                     /locus_tag="VFK23_01895"
     CDS             complement(6208..6489)
                     /locus_tag="VFK23_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317865.1"
                     /translation="MAETGFKGKSMKAVAVDVAEGYISVNPLLIKKFDKDMVKNLYEN
                     LNKALTATRNEKVVLTDHVALRTRNLKLQRLHNAMIVLRNIAREKSIAL"
     gene            complement(6498..7055)
                     /locus_tag="VFK23_01900"
     CDS             complement(6498..7055)
                     /locus_tag="VFK23_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317866.1"
                     /translation="MTRPGKRHFIGNRGKGYLQTSGMQSRFIIFLIFLLIAYSVLLRI
                     FQKLAEIVQFPFFLPITLLTLLVFIGIVGIIYSHTFIGPINRIRNALEQLASGESDVS
                     LRLREADDPTLKDLVRTIGRLCESSKSSRVLIGEVAEDLFKDLAELRESLQRGAEKSE
                     IEKKLDDLRRDQELLDKALKAYGRM"
     gene            7131..8219
                     /gene="moaA"
                     /locus_tag="VFK23_01905"
     CDS             7131..8219
                     /gene="moaA"
                     /locus_tag="VFK23_01905"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HET7317867.1"
                     /translation="MIILANQWPVKALVCLPVVFCLEERVSLWYSCGMALVDSHNRRI
                     DYLRISVTDRCNLSCVYCKPRMQMQALPHREILSYEEILRLVAIAAPLGISRVRITGG
                     EPLVRRGVAGFIASLTRFDGIEDVSLTTNGVLLENMANALVKAGRPRLNISLDSLKAQ
                     RFREITGRNDWEAVWRGINRAEELGFFPLKINMVPVRGMNDDEVADFARLTLDRRLQV
                     RFIEFMPIGADDRWRRDVCVTSEEVRAAIERELGPLIPFAAAPSAGPSTNFQVPGAQG
                     VIGFISPITRHFCGSCRRLRLTADGKIRPCLLSDTEIDIKSPLRSGSSDAVIEGLLRT
                     ALEIKPERHHLAERGPECFQRTMSRIGG"
     gene            8371..>8670
                     /locus_tag="VFK23_01910"
     CDS             8371..>8670
                     /locus_tag="VFK23_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HET7317868.1"
                     /translation="MSKLTHLNDRGDARMVDVSGKDKTPREAVAEGRVTMLPETLSLI
                     LDHKVAKGDVFAVARIAGIMAAKKTSELIPLCHPIALSSVEIDFEPDHAGSCVNIR"
BASE COUNT         1746 a         2678 c         2518 g         1728 t
ORIGIN      
        1 gactttaaac tgaagtgagt tccgcggcgg acgaacctct ccggaagcaa ataaaaagcg
       61 ggctgatccg cagtggaagt cagcccgcat gttcaatatc ccgccccgtt ttttcgacta
      121 cttctccatc cccgcccgga acgtatcgag ccacctgatg tgggccttct cctcgttgat
      181 gacctcgtcg acggccgctt tatccttgac gatcgtccgg agttcgagga aatagagcag
      241 cgtctctttc tcgaagccga tcgcgaacct caccgcgtcc ttcaccgact tgatatggtc
      301 cagggacggg agggacttgc ccttccccag gaagaactcc gactcgacga aggcccgcat
      361 gtaattgctc acctcttccc actggaccgg ctcggcgccg ttcttcgtga cagcatccct
      421 gatgccttta aaggtcttct cgtgctcctt ttcctttctc gccagatcgg tgaagagctt
      481 cactagcccg tcgtccttct tgaacttctc cgccattccc gtatagaact gggtgccgag
      541 cacttccgtc tgaaccgcca tttccatgat ttcgtcgatc gaatacctgt tcatcgtaat
      601 ctccttgtcc cgtatgagtg gaatgattcc gccggcagcg gattacggat aaatacttga
      661 ggcaaataat atcttggaac ggggggcagg tcaaccccga tattaacgcc ataaagattt
      721 cttgtcattc ccgcgcaagc gggaatccat tttgaatttc aactgatgac agccaatgga
      781 tacctgcttg cgcaggtatg acagacgggc aaaacgcctc agcctcctat cctcgtgccc
      841 gcctgccccg cgagggccgc ccccatgagc tcgggccgcg tgatgacgac ctccctgccc
      901 ccggcttcga gaaactcgat cgcagcctct atcttcgggc ccatgctccc cggaggaaaa
      961 tgcccttcgg caaagtagcg cttcatttcc acgaggctcg cccggtcgag gcccttctcg
     1021 cccgttgcgc catagttcag atagaccctg tccacctcgg tcaggatgat gatgccctcg
     1081 gccccgatct ccctcgcgag ggcggaggtc gaaagatcct tgtcgatcac ggcgtcgatg
     1141 ccccgcagca acccctccgg ggtctccaaa acgggcacgc cgcccccgcc ggcggctatc
     1201 accacgatgc cttcgagggc gagccttttg atgacgtcct tctcgatgat ccgcaccggc
     1261 ctcggcgaag gcaccgccct ccggtaaccg cccgtcccct tgaccatcgt ccaccctttc
     1321 tcgctccgca gcgcctccgc ctcgtcttca cggtaatagg gaccgatggg ttttgtcggg
     1381 ttcagaaaag cgggatcgtt ctcgtcgacg accacctgcg tgatgatcgt cgccacgtcc
     1441 cgtttcccgc cgatcttcct caggctgttg taaagggtct gctggatcat gaatccgatg
     1501 cccccttccg agtcggcgtc gttcatgaag agcggcatgg gcggaatggt gtttctcgcg
     1561 cactcgttcc tgatgacgat attgcccacg atgggcccgt tgccgtgggt gatgatcacc
     1621 ctgcgcccgg ccgcgagcat tttcgcgacc tcggccatgc aggcgtcggc atgggcaaac
     1681 tgctcctcga cggtgccctt ttcgccgcgc cggatgatgg cgttaccgcc cagggtgact
     1741 atggtgatct tgttatccat acggttagcg gatcaagcac caaacctcaa atgacaaaat
     1801 ccaaataata tccaatggcc aaaaaaacaa atgagcgaaa cgtttggaat ttgacatttc
     1861 atatttggaa tttacttggg atttggcgct tgtattttgg aacgtgctcc ttgaaggagc
     1921 caattctcga tctccttctt cagcggagcg ccctccatct cctcaatatc ataggtgacg
     1981 cggacggtct cctttgagag ttcaagcagg gccttcaggt ccacgggcgt cgcaatgagc
     2041 acgagatcgc agggcgtgga ctcaatggtc tccttgagtt cccgcatctg ccggtccgag
     2101 tagcccatgg caggaaccag atttccgatg tggggatact cctcgaaggt ctcccgtatc
     2161 gagcctatcg caaaaggcct cggatccacg agctccgcgg cgccgtagtc acgcgcggct
     2221 atgaccccgg ccccgtacgc catgccgccg tgggtgagcg tggggccgtc ttcgacgacc
     2281 aaaacccgtc tgccccggat cgcgcccgga ttgtcggcgc gaacacggga agccgtgtgt
     2341 attatccgtg cggatccgtt gattgcacgg atgttgtctt ccaccttcgc cacggcgtcc
     2401 ctgccggcgg tattgacctt gttgatcacg gcgatgtggg cccgacgcag attggcctcg
     2461 cccggatgaa aggtgatctc atgccccggc cgcagcgggt cgaggacgac cacctcgagg
     2521 tccggccgca ggaacggcag gtcgttgttg cccccgtccc agagcaggac atcggcctct
     2581 ttttccgccg cttcgacgat cgcctcatag tccacgccgg cgtagacgac aacgccggcg
     2641 ccggccagcg gttcgtattc ctcccgttcc tcgatcgtac agccgcaggc ccgcacgtcg
     2701 ctcatgctgc cgtaccgctg cactctgttc ttgatcaggt cggaataggg catgggatgc
     2761 cgtatagcaa ccggtcgcat ccccattccc atgaggaccc cggcgatata tttcgtgata
     2821 ccgcttttgc ccgcgccggt ccgcacggcg cagaccgaca gaacgggccg ggatgattcg
     2881 atcatggtct ttttcgggcc aaggagagta aaatcgcaac caagggccat gcagagcgat
     2941 gccttgtgca tgacgtactc gtgcgacacg tcgctgtagg aaaaggcgac ctggtccacc
     3001 cggaatttcc cgatgagttc gggaagctcc tcttcgggcc ggatagggat gccctgcgga
     3061 tagagggggc ccgagagccc ggggggatag agccgccccc cgatcatagg tatctgggct
     3121 gcggtgaagg ccacgacctc gaagtccgga tcgccccggt agaccatgtt gaagttgtgg
     3181 aagtccctcc ccgcggctcc catgatgatg acgcgctgtc gtttcatgtc aagtgccctt
     3241 atcattttgt cttgatgttg gttaaaagta tatcaatttt tttagcacaa tcgtgacttg
     3301 cttgtcattc ctgcggaagc aggaatccat tttaagactt gctctgaaaa aagccaatgg
     3361 atacccgctt cagcgtggcc cgcttcatcg gccatcgcgg gtatgacagc atgaggtaat
     3421 cctaccggta ctgcactcgt ccccgcgtgg tgtgcaggtc gatcgcggga cctcccttcg
     3481 ccgtgagcag gccgcccgtt tcatcgatga cggtgatgtc cccgcggcgg gcgcgcaccc
     3541 tgatcgtctt gatatcccga accggcacgc ggaattgtcc tacgccgtcg atgtcggtga
     3601 accagccttt gagggagacg cggtaccgca gggcgggaac gggcccgtcc gtaaactccg
     3661 ccgacgagtc gatttcattg cccggcaggc cgaagccgtg gacgacgccc gagcacgcga
     3721 tctctccttt cgtgtccgct accaggtaca gttccacatc gagctggtca acgagcactt
     3781 cgagattccg cggcgcggaa agatgcgaag gaggcttgca gggccgcaac tcctcataat
     3841 aggggttcgc aagcatgccg ccaccgatga tcaggaggcc gacgaagatg gcggcgacaa
     3901 tcacagccgt cttcatccgc ctgctgccca cgatgagcgg gtagagcgcg gcgcggccgt
     3961 cttcgcgcgc cgggacccgg agcgcgtagt agtagaggcc gtgtgcgatg ccccaggcca
     4021 ggagcgcgga ccacacggca tcggacagaa agtgagcgcc cccggccatg cgtccgattc
     4081 ccagcagggt gccgaggatc aggccggaga cgagagaagc gacggccagc cgcggccggc
     4141 gccgcctcca cacccaccag cccaccgcgt agagatatcc caccgaacag tggccgcagg
     4201 gaaatgattt gccgtatgat acggtcggaa caaagggctg cgtgtattcg agccgccccc
     4261 cgaactcggt gatctgccgg ggccgcggcc gcccccagtg gtccttcaat acaacgttga
     4321 tgatgagccc gggccctacg gcgacgcaga gaaggatgaa aagcccatac agccgcagac
     4381 gtttcgaccg ctcccgcagt acgccgatga tggacagact gacgcccgcc accgccagcg
     4441 acgccgtcac ccagggcgcc gaccggtaga acagcgccca gaccgggtgg ttcgccacgg
     4501 gccagggatc ggccgactca ggatgataga acggccgcat ggtagtgatg tcgaggtccg
     4561 tggccgcgaa cagaatgatc gcgccgaggg cggcagccgt caggacaaca agttcgggaa
     4621 gccagtagcg gcggtatgcc acgatggggc cctgcggctg attcgtaacc atgttctcat
     4681 cccctgctgt aagtatagcc ctcggaacag catcgggagg tcccggtcca cgggacatta
     4741 tcattttggc ttgacaaccg cctcaatgcg tattgacaat tccgccttga tttgctacag
     4801 ttgcgcgtgc gaacggcacg aggacaaatc ggataacacg aatcgatcag gaggcaatca
     4861 tgggagacaa actcgcggtg gtagtcggcg gagggcccgc cccggggatc aacggggtga
     4921 tcagcgccgt gacctatgag gcggccaagc agggcctcga ggtcgtcggc gtgcacgacg
     4981 ggttcaagtg gctttctaag ggcgacaccg gccacacgac gaacctgtgc aagagcgaca
     5041 tctcgcgcat ccacatgcgc ggcggatcgg tcatcgggac ctcgcgcgag aacccgacga
     5101 aaagccccga gaagatggcc aacgtcatca aggcgctgac ggagctcggg ataaaatatc
     5161 tcgtcaccat cggcggcgac gacacggcct ttaccgcgac ccgcgtcgaa caggaggcgg
     5221 gcggcaggat ctcggtctgc cacgttccca agaccatcga caacgacctt cccctccccc
     5281 acggcgttcc gaccttcggc ttcgagaccg cgcgctccct cggcacccag ctcgtggaaa
     5341 accttatgga agacgcccgc accgcggcac ggtggtactt cgtcatcgcc atggggcggt
     5401 cggccgggca cctggcgctc ggcatgggca tgggagcggg tgcgacgctc acgctcatcc
     5461 ccgaggaact cgggagcaag atatcgctca cgaaggtcgt gtccatcctc gaaggcgcca
     5521 tcattaagcg cctctcgctc ggcaaaggcc acggcgtcgc ggtcatcgcc gagggcgtcg
     5581 cgacgcgctt cgatcccgag gaactgacct ttctcaagga cgtgccccgg gacgaccacg
     5641 gcaatatccg cctggccgag ataccgatcg gcgacatgct gaagcagcgg gtcatgacgg
     5701 acctcgcttc catcgggctc aagaccacgg tcatcgccaa ggacatcggc tacgaactgc
     5761 gctgccatcc gcccatctcc tatgaccgcg agtatacgcg gtacctgggc ttcagcgcgg
     5821 tgaagttcct cgtctcaggc ggctcggggg ccatgatcat gctcaaggag ggcgtcagcc
     5881 tgcccatcaa gttcacggac ctgctcgatc ccaaaacggg gaagacgagg atccgcactg
     5941 tggacatcaa ctccgacgat taccacattg cacggcgtta catgatcagg ctcgagaagg
     6001 aagacatgga aggcgagggg atcaagaagg tcgctgccgc ggcgaagatg gctcctgagg
     6061 actttaaaaa gaggttcggg gtggcggtgg ggtaaaaaca gttcggagct gggagttcgg
     6121 agttgggagt ggggaagatt aaagccaaga aatgcagtga tgtggtctca acactccgag
     6181 ctctgaactc cgaactcatc actgtatcta tagtgcgatg ctcttctccc gtgcgatgtt
     6241 tctgaggacg atcatggcat tgtgcagacg ctggagcttc aagttccgcg tcctgagcgc
     6301 cacatggtcc gtgaggacga ccttttcatt cctcgtggcc gtcagcgcct tgttcagatt
     6361 ttcgtagagg ttcttgacca tgtccttgtc gaacttcttg ataagcagcg ggttgaccga
     6421 aatgtagcct tcagccacat cgacggcgac cgccttcatg ctcttgcctt taaacccggt
     6481 ctcagccatg accgctccta catcctgccg tacgctttga gagccttgtc cagaagctcc
     6541 tggtcccgac gcagatcgtc aagctttttc tcgatttcgg acttctccgc gccccgctgg
     6601 aggctctccc ggagttccgc aaggtccttg aagagatcct ctgccacctc cccgatcagg
     6661 acacgggagc ttttgctgct ttcgcacagg cgcccgatcg tccttaccag atccttgagc
     6721 gtgggatcgt ccgcttcccg gagcctcagg gaaacatcgc tttctcccga tgccaactgc
     6781 tccagcgcgt tgcgtatccg gttgatgggg ccgatgaagg tgtgggaata gataatgccg
     6841 acgatgccga taaataccag aagcgtcagc agcgtaatgg gcaggaagaa gggaaattgc
     6901 acgatttccg cgagcttctg gaaaatccgg agcaggaccg aatatgctat caggagaaag
     6961 atgagaaaaa tgatgaaccg ggactgcatg cccgatgtct gcagatagcc cttgcccctg
     7021 ttgcctataa aatgtctctt ccccggcctg gtcatgaggt ccttggcgcc ttgcgccctg
     7081 gaaacgatgg tagatgacag cccgcgttca cccggcagta cggaaacaat atgattatac
     7141 tggcaaatca atggccggtc aaggccttag tttgcctgcc ggtggtgttt tgccttgaag
     7201 aacgggtatc gctgtggtac agttgtggca tggcccttgt cgactcacat aatcgccgca
     7261 tcgactatct gcggatatcc gtgaccgacc ggtgcaacct ctcctgtgtg tactgcaagc
     7321 cccggatgca gatgcaggcg cttccacacc gcgagatact ctcctatgag gaaattctga
     7381 ggctcgtggc catcgcggcg ccgctcggca tctcccgcgt caggatcacc ggcggtgagc
     7441 cgctggtcag gcgcggcgta gccggcttca tcgcatccct gaccaggttc gacgggatcg
     7501 aggacgtgag cctcacgacc aacggcgtgc tgcttgaaaa catggccaat gccctggtaa
     7561 aagccggcag gccgcggctc aacatcagcc tcgactccct gaaggcgcaa aggttccgcg
     7621 agatcaccgg ccggaacgat tgggaggcgg tatggcgcgg catcaaccgc gctgaagaac
     7681 tcggtttctt tccgctcaag atcaacatgg tgcccgtccg cggcatgaac gacgacgagg
     7741 tggccgattt cgcccgcctt acgctcgacc gcaggctcca ggtccgcttc atcgagttca
     7801 tgcccatcgg cgcggacgac cgatggcgcc gcgacgtctg cgtcacctcg gaagaggttc
     7861 gtgccgccat tgaacgggaa ctgggccccc tcatcccctt cgccgcggcc ccttccgccg
     7921 gcccttcgac caacttccag gtgccgggcg cacagggagt gatcgggttc atcagcccca
     7981 tcacccggca cttctgcggc tcctgcagac ggctgcggct tacggccgac ggcaagatac
     8041 ggccctgcct cctgtcggac acggagatcg acatcaagtc gccactccgg agcggatcga
     8101 gcgatgcggt aatcgaaggg ctcctgagaa ccgcgctcga aatcaagccg gagcggcatc
     8161 atctggcaga gcggggaccg gaatgcttcc agaggacgat gtcgaggatc gggggatagt
     8221 tagagcaagt accaatctcc aaatcgcaaa ttccaaataa cattcaaatt cagggcgcca
     8281 atgaccgaaa cgtttggaat tttgaaattg ggggttgaaa tttatttggg atttgggatt
     8341 tgatttttgg gatttgaaat ttagcagcct atgtccaaac tcacccactt gaatgaccgg
     8401 ggcgacgcgc gcatggtcga tgtgtccggc aaggacaaaa ccccgcggga ggccgtggca
     8461 gagggccggg tcaccatgct gcctgaaacg ctttctctca tcctcgatca caaagtcgca
     8521 aagggagacg tcttcgcggt cgcccggatc gcaggcatca tggccgcgaa gaagacctct
     8581 gaactgatcc ccctgtgcca cccgatcgcc ctctcatccg tggaaatcga cttcgagccc
     8641 gaccatgccg gatcgtgcgt gaacatccgg
//
LOCUS       DASSLD010000102         3668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1358792, whole genome shotgun sequence.
ACCESSION   DASSLD010000102 DASSLD010000000
VERSION     DASSLD010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358792"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1307
                     /locus_tag="VFK23_01915"
     CDS             252..1307
                     /locus_tag="VFK23_01915"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET7317869.1"
                     /translation="MKTPIIQGGMGVGVSLHPLAKAVAREGGLGIVSSACLDRLVSKR
                     NNKKVNIYEAAYEEVSLSKTPGGYVGINIMGALARDFNDSVKGALDAGADAIISGAGL
                     PLNLPAIQPPKDTALIPIVSSARALEIICKKWVKLGYRPDAVVLEGPLAGGHLGFRID
                     QITLESNKLENLFPSVKDTAMKYGDFPVIVAGGLYTHNDIVQFMNEGADGVQMGTRFL
                     ATEESSASTPYKEAVILAKEEDIIVAHRPGSPCGLPFRVIKQSPMYVSSLQQLRKPKC
                     DKGYVLMKDAEGKFTICPAKEDNKSYFCICNGLLSSSGYNPIEEEPLYTVGTNASRVD
                     KILPVKSLMHELTGGGV"
     gene            complement(1404..2498)
                     /gene="ychF"
                     /locus_tag="VFK23_01920"
     CDS             complement(1404..2498)
                     /gene="ychF"
                     /locus_tag="VFK23_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET7317870.1"
                     /translation="MGFNCGIVGLPNVGKSTIFNALTSAGAQASNYPFTTIDPNVGVV
                     DMPDERLDRLAAIYHPKKLTPTTMEFVDIAGLVRGAAQGEGLGNKFLANIREVDAIAH
                     VVRCFDDPNVVHVAGKVDPKADIEVIEAELMLADLDAVEKRLFKAEKIVKTGDKKAGE
                     EVEFMRRLKDMLAKGEPIRRATHSDEEALWLKGYNLLSAKPVLYVANVAEDMVDKPNV
                     YVDTVKKIAGEEGAKVVVISGQVEGEIAQLPKEERKEYLSGMGLRESGLDRMIRAGYD
                     LLGLITYFTAGEKEVRAWTITRGTKAPQAAGKIHTDFEKGFIRAEVFHYDDVKNLGAE
                     QAVKAAGLLRSEGKDYVVRDGDIMLFRFNV"
     gene            complement(2692..3255)
                     /gene="pth"
                     /locus_tag="VFK23_01925"
     CDS             complement(2692..3255)
                     /gene="pth"
                     /locus_tag="VFK23_01925"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET7317871.1"
                     /translation="MKIIVGLGNPGRKYERTRHNAGFSAIDGLARDLQSEVMKEKHHS
                     LVGRARIGSEEVVLAKPQTYMNESGRAAAAIMRAVYATPADLIVIHDELDLPLGSVRV
                     KIGGGHGGHNGLRSVIEHLGSSDFVRVRIGIGRPATGMDAVDYVLSPFLIEEKKSLSD
                     AMTRVVAAVKTIILDGPTRAMNMFNQK"
     gene            complement(3290..>3668)
                     /locus_tag="VFK23_01930"
     CDS             complement(3290..>3668)
                     /locus_tag="VFK23_01930"
                     /inference="COORDINATES: protein motif:HMM:NF026044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317872.1"
                     /translation="GGIFQYGLRTLEIECTPDLSPDSIEVNVSELKVNESLHVRDIRA
                     AEGIRVLTDGDSTVATIQPPISEAKLEAMLTATPAVAAEAGEPELVKKPKKEGEEAAA
                     PAAEAPKGKEGAKEATKEAAPKK"
BASE COUNT          779 a         1060 c          975 g          854 t
ORIGIN      
        1 taagcttttt gttcctccag ccggctttcc atgaaggatc gtcaaagacg ttgcgtgtaa
       61 aaaccattat cacaacaccc cgactttctc aaagatgttt tattctggag tgagcaccgc
      121 acggaatacg acagaatttt aacagtaaga atcgaagatt ccccggagtt tttcccgggg
      181 aaccttaatg tcaaggggga attttgaaac ttgattcagt attaaagcca ctcattatca
      241 aaggaaaacg cgtgaagacg cctattattc agggtggaat gggtgtcggc gtctcactcc
      301 accccctggc gaaagctgtg gctcgtgaag gcgggctagg catcgtttcc agcgcatgcc
      361 tggacaggct cgtttcgaaa agaaacaata agaaagtgaa catctatgag gccgcctacg
      421 aggaagtttc cctttccaaa acacccggcg gctatgtggg gatcaatatc atgggcgccc
      481 ttgcgaggga ttttaacgat tcggtcaagg gagcccttga tgcaggcgca gacgccatca
      541 tctcgggagc tgggctgccg ctgaacctgc cagccattca gccacccaaa gatacggccc
      601 tgattcctat cgtttcgtcg gcgcgtgcac tggaaatcat ctgtaaaaaa tgggtaaagc
      661 tgggttaccg tcccgatgcc gtcgtgcttg aggggcctct cgcgggcgga cacctcggtt
      721 ttcgaattga tcaaatcact ctggaatcaa acaagcttga gaacctattc ccctcggtaa
      781 aagacacggc catgaaatac ggagatttcc cggtcatcgt cgccgggggg ctttacaccc
      841 ataatgatat cgtccagttt atgaacgagg gtgcggacgg tgttcaaatg gggacccgct
      901 ttcttgcgac tgaggaaagc agcgcttcga cgccttataa ggaagccgtt atcctcgcga
      961 aggaagagga catcattgtg gcgcaccgac ctggatcgcc ctgcggtttg ccgttccggg
     1021 tcatcaagca atctcccatg tatgtctctt cgcttcagca gctgcggaag cccaaatgcg
     1081 acaagggcta cgtcctcatg aaggatgcag aaggaaaatt tacaatctgt cccgccaagg
     1141 aagacaacaa gagttatttc tgcatctgca atgggttgtt gagttcaagc ggatacaatc
     1201 cgatcgaaga agaaccgctc tatacggtgg gcaccaacgc ttcccgggtt gataagatcc
     1261 ttccggtaaa gtcgctcatg catgaattga ccggtggggg agtctgatcc gaccagccgg
     1321 gaacagttgt tagctgccaa atgaaaccac caagaatatt tcacgaccgg aacctatttg
     1381 aagcacaacc gcgcgactcc cggctacaca ttgaaccgga acagcatgat gtccccgtcc
     1441 cttacgacat aatccttgcc ttcggagcgg agcagacccg ccgctttcac ggcctgttca
     1501 gccccgagat tcttgacgtc gtcgtagtgg aaaacctcgg ctcggataaa gcccttttca
     1561 aaatccgtgt ggatcttgcc cgcagcctgg ggggccttcg tgcccctggt gatggtccag
     1621 gcccgcacct ctttttcacc cgcagtaaaa taggtaatga gccccaaaag atcgtagccg
     1681 gcgcggatca tgcggtccag cccggattcc ctaagcccca tgcctgagag gtattccttc
     1741 cgttcttctt tcggaagctg ggcgatctcg ccttccacct gtccggaaat gacgacaacc
     1801 tttgcgcctt cctcgcctgc tattttcttc accgtatcga catagacatt cggcttatcc
     1861 accatgtctt cggccacgtt cgccacgtac aggaccggtt tggcagacag gagattatag
     1921 cctttgagcc atagcgcctc ttcgtcggaa tgggtcgccc ggcggatcgg ctcacccttc
     1981 gcgagcatgt ctttgagccg ccgcatgaac tccacttcct cgcctgcctt cttgtcaccg
     2041 gtcttgacaa tcttttcagc cttgaacaag cgtttctcga cagcgtccag atccgcgagc
     2101 atgagctctg cctcgatcac ctcgatgtcc gccttggggt ccaccttgcc agcgacatgc
     2161 accacattgg gatcgtcgaa gcagcgcacg acatgcgcaa tggcgtccac ctcgcggatg
     2221 ttggcgagaa acttgttgcc gagtccttct ccctgggccg cgcccctgac caggccggcg
     2281 atgtcgacga actccatggt cgtgggcgtc agttttttcg gatgatagat tgccgccagc
     2341 ctgtcgagac gttcatccgg catgtccacc acgcccacgt tcgggtctat ggtcgtgaag
     2401 ggatagtttg acgcctgtgc gcccgcagag gtcagcgcat tgaagatggt ggacttgccg
     2461 acattgggaa gtcctacgat gccgcagtta aaacccatgg aaaaaacctt tctgcattgg
     2521 acgcggattt acgctgataa actcagatta aagacagtca agacagcgac atttctcgtg
     2581 gtggttttgt tatccggttg acgccgtaac tttctcggtg tccttagtgg tggcggaaaa
     2641 ataaaaaccc ggagctgtcg gaatagtcct tcaggtccgg gctttgaacc gttacttctg
     2701 attgaacata ttcatggccc gcgtcgggcc gtccaaaatg atggtcttaa ccgctgcaac
     2761 gacccgcgtc atcgcatcgg acagcgattt tttctcctcg atgagaaacg ggctcaggac
     2821 ataatcgacg gcatccatgc ccgtcgcagg ccttccgatt ccgatacgca cccttacgaa
     2881 atccgacgac cccagatgct cgatgacgga gcggagccca ttgtggccac catggccgcc
     2941 gccgatcttc acccgcactg acccgagtgg aagatccagt tcatcgtgga tcacgatgag
     3001 gtcggccggt gttgcgtaca cggccctcat gatcgcggca gcggccctgc cgctttcgtt
     3061 catataagtc tgcggttttg ccaggacgac ttcttcggaa ccgatccggg ccctgccgac
     3121 aagcgagtga tgtttttcct tcataacctc gctttgaagg tcccgggcaa gcccgtcgat
     3181 tgccgaaaat cccgcgttat gtcgtgtgcg ttcgtacttt ctgcccgggt tgccgagccc
     3241 gacgatgatt ttcattcaat acaatacccg gagaccagcg cacgtggtct tacttcttgg
     3301 gggctgcttc cttggtagct tccttggccc cctccttgcc cttcggcgcc tcggcggcag
     3361 gcgcggcggc ctcttcgcct tccttcttcg gcttcttgac aagttcgggc tcgccagcct
     3421 cggccgctac cgcgggggtc gcggtcagca tggcctcgag cttggcttcc gaaataggcg
     3481 gctggattgt ggcgaccgtc gaatcgccgt cggtcaggac cctgatgcct tccgccgccc
     3541 tgatgtcccg aacatggagc gactcattga ccttgagctc tgagacgttg acctcgatgg
     3601 agtcgggact caggtccggc gtacactcga tctcgagcgt ccggaggccg tactggaaga
     3661 taccgcct
//
LOCUS       DASSLD010000103         3620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1554883, whole genome shotgun sequence.
ACCESSION   DASSLD010000103 DASSLD010000000
VERSION     DASSLD010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554883"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="VFK23_01935"
     CDS             <1..1516
                     /locus_tag="VFK23_01935"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7317873.1"
                     /translation="TQGASSMDGSSGKRVCVIGAGIAGLVTSKVLKEDGFEVVVFEKQ
                     PEPGGVWASSRTYPGLRTNNPRETYAFSDYPYPESTDDFPSAEQVRSYLGSYAEHFGL
                     RPLIRFSTEILSVVGVANGTGEARTFKVVSRTEGGKTAETLLFDYVVICNGVFSTPKI
                     PRVVGQELFEGTVFHSSRLTDPRAVEGRRVVVVGAGKSALDCAAWAAEHGESCTLVFR
                     SPHWMAPRYFFGRIRFDRMVMTRSAESFLRYHRMSRCERVLHGPAGPFVRAWWRGYGC
                     LLKRLLKMPPVLTPELRLPLGFEYLGVGGEMYKVLDSGKLVLKRAGLSRFTDADEIEL
                     DTGEAVKADVVVFATGYRLDLSFLEPGLLSQVQRNGQLRLYRHILPPRQSRIGFIGYA
                     SSTACQLTSEVAAHWLSQCFLGELVLPGAAEMEKEISRVLHWASETFPGRGEGYYIGP
                     FVAHYLDDLMGDMGLPRRRTGGFFTEYFAPFWPDRYRNVGEERRRARAGKQPHP"
     gene            1708..2865
                     /locus_tag="VFK23_01940"
     CDS             1708..2865
                     /locus_tag="VFK23_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7317874.1"
                     /translation="MGDFFSAIKNFSSFVWLPLWSALLLLPLAGPGNAVRIAVIAGLC
                     VALWRLVKSETVANFTKTMSGKLSRLPVSTGRLTSLAQSPAGLVALVILLVLLPLFLN
                     DYYRDTMTLTCLYIVLALGLNIVVGQAGLLNLGYVAFYAIGAYTYAILSTSFGVSFWP
                     GIVAGALTASLFAVLVGLPTLRLRGDYFAIVTLGLGEITRIVFNNWDSVTGGPNGISK
                     IGRPALAGYLLSGALDFYYLILAIAVFTVFAMHRLMESRIGRAWIAIREDETAAEAMG
                     INTFRLKLLAFVIGSAWAGAAGVFFAAKMAFVSPESFTFSESVLILCMVVLGGMGSIP
                     GIVLGAFLLITLPEVFREFQDYRMLAFGAALVTMMVFKPQGLLGAVKTNTE"
     gene            2969..>3620
                     /locus_tag="VFK23_01945"
     CDS             2969..>3620
                     /locus_tag="VFK23_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7317875.1"
                     /translation="MLLLETKNLTKHFGGLSALDDIDMRLERERIVSIIGPNGAGKTT
                     FFNCVTGMYQPTKGTVTFHDRDVTRMPPHEITTLGVARTFQNIRLFGGMSCLENVMVA
                     AHCRTTAGVFGAIFRPEKVKREERDMVFRAMDILKFVGLENKHDAPAANLSYGDQRRL
                     EIARALGTEPHLLLLDEPAAGMNPQETQELMALIRKIRDNGVAILLIEHDMKVVMGI"
BASE COUNT          670 a         1065 c         1085 g          800 t
ORIGIN      
        1 cactcaagga gcgtcatcca tggacggatc ttccggcaag cgcgtgtgcg tcatcggagc
       61 gggcatcgcg ggactcgtaa cgtcaaaggt gctcaaggaa gacgggttcg aggtcgtcgt
      121 gttcgaaaaa caaccggagc cgggaggtgt ttgggcctca tccagaacct atccggggct
      181 tcgcaccaac aacccccgcg agacctatgc attctcggac tacccctatc cggagagcac
      241 cgatgatttc ccgtcagcgg aacaggtccg ttcctatctg gggtcgtacg cggaacattt
      301 cggcctgcgg cctcttattc gtttttccac cgagattctc tcggttgtcg gcgtcgcgaa
      361 cgggaccggg gaggcgcgaa cgttcaaggt cgtcagccgg accgaaggcg gcaaaaccgc
      421 ggaaacgctt cttttcgact atgtcgtgat ttgcaacggg gttttctcga cgccgaagat
      481 tccacgcgtc gtcggtcagg agctttttga ggggaccgtg ttccattcga gccggcttac
      541 agacccgcgg gcggttgagg gaaggcgcgt cgtggttgtc ggcgcaggca agtccgcgct
      601 ggattgcgcg gcctgggccg ccgaacacgg agagtcgtgc acgctggtgt tcagatctcc
      661 gcattggatg gcaccgcgct attttttcgg gcgcatccgc ttcgaccgga tggtcatgac
      721 ccgttcagcg gagtcattcc tgcgctatca ccggatgagc cgctgtgaga gggttctcca
      781 cgggcctgcg ggacctttcg tccgcgcctg gtggcgcggg tacggctgcc tcctcaagag
      841 gcttctcaag atgccgcccg tgcttacgcc ggagttgcgg cttcccctag gatttgaata
      901 cctcggcgtg ggaggcgaga tgtacaaagt tctggactcc ggaaaactgg tcctcaaacg
      961 agcagggttg tcgcgtttca cggacgctga cgagatcgaa ctcgacactg gcgaggccgt
     1021 caaggccgat gtggtcgtct ttgccaccgg ctatcgactg gacctttcct ttctggaacc
     1081 cgggcttctc agtcaggtcc aaaggaatgg tcagcttcgt ctctatcggc acatccttcc
     1141 cccgcgacaa tcccgtatcg ggttcatcgg ctacgcctcc tccactgcgt gccagttgac
     1201 ctcggaggtc gccgctcatt ggctctctca atgcttcctg ggtgaactcg tcctcccagg
     1261 tgccgcagag atggagaagg agatttcaag ggtcctgcac tgggcttccg agacctttcc
     1321 cggaagagga gagggatatt atatcggacc cttcgtcgcg cactacctcg acgacctcat
     1381 gggcgacatg ggcctgcccc gaaggaggac cgggggcttc tttaccgaat atttcgcgcc
     1441 tttctggccg gaccgctatc ggaacgtggg agaggagcgc cgcagggcgc gcgccggaaa
     1501 acagccccat ccctagaagg ttcggggacg cctatgaagc gggggaatgg aaagtcacgt
     1561 cggcgcgctg gaaccggatt gatgcggagg gaaggtgaca acttaaatcc cccttgcccc
     1621 ctcgttcgta aagagtgggg agggagacta aacatgcatg aatgcgtttg atttaatata
     1681 ttttttgtga gataatcacc cccggtcatg ggtgattttt tttcggcgat aaagaatttt
     1741 tccagcttcg tatggctgcc cctctggtcg gccctgctcc tgcttcctct tgccggtccg
     1801 ggaaacgccg tccggatcgc cgtcatcgcc ggtctttgcg tcgcgctgtg gcgcctcgtg
     1861 aaatcggaaa cggtcgcgaa tttcaccaaa acgatgtccg ggaaactgtc ccgccttccc
     1921 gtctccacgg gccgtttgac atccctcgca cagtccccgg ccggtcttgt cgctctcgtc
     1981 atcctcctcg tcctgctccc cctttttctg aatgattact accgcgatac tatgacgctg
     2041 acgtgcctgt atatcgtgct cgctctgggg ctgaacatcg tggtcggcca ggctgggctt
     2101 ctcaatctcg ggtatgtggc gttctatgcg atcggcgcgt atacctatgc gatcctctcc
     2161 acgtccttcg gtgtttcgtt ctggccggga atcgtggccg gagccctgac ggcgtccctt
     2221 ttcgccgttc tcgtcggatt gcccaccctg cggctccgcg gagactactt cgccatcgtc
     2281 accctcggcc tcggagagat cacccgtatc gttttcaaca attgggacag cgtcaccgga
     2341 ggtccgaacg gcatttcgaa aatcggcagg cctgctctcg ccggttacct gctcagcggc
     2401 gccctggact tctattacct cattctcgcg atagcggtat ttaccgtttt tgccatgcac
     2461 cggctgatgg aatcccgcat cggaagggcc tggatagcga tccgcgagga cgagacagcc
     2521 gcggaagcca tgggcatcaa tacgttccgt ctcaagctcc tggcctttgt catcggctcg
     2581 gcctgggccg gtgctgccgg cgtttttttc gccgcgaaga tggctttcgt atcgcccgag
     2641 agcttcactt tttccgagtc cgtcctgatc ctctgtatgg tcgtcctcgg cggcatgggc
     2701 agcataccgg gcatcgtcct gggcgcgttt ctgctgatca cgctgcccga agtcttccgg
     2761 gagttccagg attaccggat gctcgccttc ggcgcggccc tcgtcaccat gatggtgttt
     2821 aagccgcagg gactgctggg ggcggtgaag acaaatacgg aataataatg acgagacgta
     2881 agagttggac gcagatgaac gcagaaaaac cggattaaag gtcgatctgc gttcatccgc
     2941 gtaaatctgc gtccgaaaag attctcctat gttgcttttg gaaaccaaaa acctaacgaa
     3001 gcatttcggc ggcctctccg cattggacga catcgacatg cggttggagc gggaacggat
     3061 tgtgagcatc atcggtccga acggggcggg gaaaaccaca tttttcaact gcgtcaccgg
     3121 catgtatcag cccacgaagg gaacggtcac gtttcacgac cgggacgtca cgcgtatgcc
     3181 cccccacgag atcacgacct tgggggtcgc gcgcacgttt cagaacatcc gtctgttcgg
     3241 cggcatgtcc tgcctggaaa acgtcatggt ggccgcccac tgccggacga cagcaggggt
     3301 tttcggcgcc atattcaggc cggaaaaggt gaagcgggaa gagcgtgaca tggtgttccg
     3361 ggccatggac atcctgaagt ttgtcggact cgagaacaag cacgatgcac cggcggcgaa
     3421 cctgtcctac ggcgatcagc ggcggctgga gatagcgcgg gccctcggca cggaaccgca
     3481 cctgttgctc ctcgacgagc cggccgcggg catgaatcct caggagacgc aggaactcat
     3541 ggcgctgatc cggaagatcc gggacaacgg cgttgcgatt ctcctgatcg agcacgatat
     3601 gaaggtcgtc atgggcatct
//
LOCUS       DASSLD010000104         2361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_189198,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000104 DASSLD010000000
VERSION     DASSLD010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189198"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFK23_01950"
     CDS             <1..437
                     /locus_tag="VFK23_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317876.1"
                     /translation="IGGLFMNNKPSRMVPFVFGLILGVLGTIFAPDYVKPYLPSWVVG
                     KTTVVTGIVMAKQNKNNVLMLTVNTPQGELLASFKKKVDEIGLLVNEKDVIEFTLPKY
                     MPFIEDPKVIRVVKEQPAEPEPAPPQPGPKKGAEAGKGVQGR"
     gene            485..667
                     /locus_tag="VFK23_01955"
     CDS             485..667
                     /locus_tag="VFK23_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317877.1"
                     /translation="MKPAGRREAEWRSVEENKQTASDSQKPEKDPGYRNVLKLIILIV
                     VLVGAWYLLEWLINGK"
     gene            complement(694..879)
                     /locus_tag="VFK23_01960"
     CDS             complement(694..879)
                     /locus_tag="VFK23_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317878.1"
                     /translation="MKARYIRIAIIVIGLLLAYWVYPSGLLSTPSGSWSLEQIYRGAA
                     TLVICFWIVIFVVNVTD"
     gene            1063..2280
                     /locus_tag="VFK23_01965"
     CDS             1063..2280
                     /locus_tag="VFK23_01965"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7317879.1"
                     /translation="MRKNIVIVGGGFGGLEAAFSLKSLVDDDCVITLLDRKRHHSFIP
                     SIHEISSGKIPARRIQIPLETMLAPAGIRFVPGEVLEIDAANRRVTAAAGSLDYDYLV
                     VATGAENNFFGVPGAEEHAFRFRTPDDAVMIHEHLDLLLAKERNDIHLVLAGGGTEGV
                     EVAGEFLDLIRDSGRGEDLARGKVTISLVEAQEQILPGFPPAARTFAENYLREQGVTI
                     ITGKRITAVGERSVVLATGTELPRSLLVWTGGIKPSRLIDSLRLPKDETGWLVVNDCL
                     NSRADDRVYGVGDIAAIQGPEGPLFLQRLAYHAQDQAVAAAINISYDLRGKTLMRYAP
                     RYKPKLVSIGRDTGIYTWGDEFKVGMWVVGVKKAVERTHLMSCLTRPLLTGVSRRIPG
                     NDILKRLGLKLPF"
BASE COUNT          520 a          693 c          719 g          429 t
ORIGIN      
        1 gaattggagg ccttttcatg aacaataagc cgtcacgcat ggttcctttc gtcttcggac
       61 tgatactggg ggtcctggga acgatcttcg cgcccgacta cgtgaaaccc tacctcccgt
      121 cctgggttgt gggaaagacg accgtggtca cagggatcgt catggcaaag cagaacaaga
      181 acaacgtcct catgctgacc gtgaatacgc cccagggcga gctgctggcc tcgttcaaga
      241 aaaaagtcga cgagatcggc ctcctggtca acgaaaaaga cgtgatagaa tttaccctgc
      301 ccaagtacat gccctttatc gaagatccga aggtcatacg ggtcgtgaaa gaacagccgg
      361 cagagcccga accggcgccc ccgcagccag gcccgaagaa aggggcggag gcagggaaag
      421 gcgttcaagg acgctgaacg ttgaactccg cagggaagca gcttaaaccc ttcgggggat
      481 accgttgaag ccggccggga gacgagaggc ggagtggcgt tccgtggaag agaacaagca
      541 gaccgcgtcc gattcgcaaa aacctgaaaa ggaccccggc tacaggaacg tcctgaagct
      601 gatcatcctc atcgtggtcc tggtaggcgc ctggtacctg ctcgagtggc tgataaacgg
      661 gaaatagaag gggaaggctg atgaggaccg gctttaatca gtcacattca ccacgaagat
      721 cacgatccag aagcaaatga caagagttgc ggccccccga tagatctgct ccaggctcca
      781 ggaacctgat ggcgtgctca gcaggccgga agggtagacc caataagcca gcagcaggcc
      841 aataacgatg atcgcaatgc ggatatagcg cgctttcata tggattccga cccctgaaat
      901 tatgatagca cgttccaagc ctcatcccgc atcggccgtc ctcgtccttc atttccattc
      961 cgcattactt aatcccatgg ttgaacaggc acggataacg cgcgttaccc ttaacctctc
     1021 gggaccgggt ctgctacaat taccctcata gggagagcgg aagtgagaaa gaatatcgta
     1081 atcgtgggcg gcggattcgg cgggctggag gcggcctttt ccctcaagag cctcgtcgat
     1141 gacgactgcg tgatcaccct cctcgaccgg aagagacatc actcgttcat cccgtccatc
     1201 cacgagatca gctccggcaa gattccggcc cggaggatcc agatacccct cgagacgatg
     1261 ctggcgcccg cgggcattcg ctttgtccct ggcgaggttc tggagatcga cgctgcgaac
     1321 cgccgggtga cggctgcggc cggttcgctc gactacgact acctcgtcgt ggcaacgggc
     1381 gcggagaaca atttcttcgg cgtgcccggc gcggaggagc atgcgttccg cttccgtact
     1441 ccggacgatg cggtcatgat ccatgagcat cttgacctcc tgctcgcgaa agaacgcaat
     1501 gacattcacc tggtccttgc aggcggcggg acggaagggg tcgaggtcgc gggcgagttc
     1561 ctggacctga tcagagacag cgggcggggg gaagacctgg cacgggggaa ggtcacgatt
     1621 tcgctggtag aggcgcaaga gcagatcctg cccgggttcc cgcctgcggc gcgcacgttc
     1681 gcggagaact acctgcggga gcagggcgtc acgatcatca ccggcaagcg aattaccgcg
     1741 gtaggcgagc gctcggtcgt cctcgccacg ggcacggaac tgccccggtc cctgctggta
     1801 tggacaggcg gcatcaagcc gtcgcggctc atcgacagcc tgaggctccc gaaagacgag
     1861 acagggtggc tcgtcgtgaa cgattgcctc aattcgcgcg cagacgatcg ggtctacgga
     1921 gtgggcgata tcgcggccat ccagggtcct gaaggacctc ttttcctgca gcggctcgcc
     1981 taccacgccc aggaccaggc cgtggcggcg gccatcaaca tatcctatga cctgcgcggc
     2041 aagaccttaa tgcgctatgc gccgaggtat aagcccaagc tggtatcgat cggcagggac
     2101 acgggcattt acacctgggg ggacgaattc aaggtgggga tgtgggtcgt aggggtgaag
     2161 aaggccgtgg agcgcacgca cctgatgtcg tgcctcaccc ggccccttct cacgggcgtc
     2221 tcgcggagaa tacccgggaa cgacattctc aagcgcctgg ggctgaagct tcccttctga
     2281 ccgcgccttc ccggacaaac gaaggggacc attctcctgt tccttttccc ataatgtcat
     2341 tcagcgtctc ccgcgtgctc c
//
LOCUS       DASSLD010000105         3177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_152365,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000105 DASSLD010000000
VERSION     DASSLD010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152365"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VFK23_01970"
     CDS             <1..932
                     /locus_tag="VFK23_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7317880.1"
                     /translation="YSRDEVVGRSHMAMFHGAANGVSCPFIEHALVKQERIIAEEVAA
                     RKKSGEDVTVVVTGSPIYDDAGELAGAVEIFRDVTDIKRRERERKNILSMFAHDMKNP
                     VTVSGGFLARLLSDKAGALTEKQRQYLETIREELYKVSDLLADFLEFSRIEARAYIPV
                     FRPFDIEETVRRNIEASKVAADRKNIAVSLEVTGEVSSSILADPSMIDRVIANLLDNA
                     IAYTDAGGIVRVRISGREGAVLVSVEDTGRGISKDQIPYLFDAFYRASRDSKGSGLGL
                     FIAKTNIESHGGRIWVESTPGKGSTFSFTLLRR"
     gene            complement(964..1575)
                     /locus_tag="VFK23_01975"
     CDS             complement(964..1575)
                     /locus_tag="VFK23_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HET7317881.1"
                     /translation="MNTLIRTIEVLGIIAFALTGFYAARKKGMDLIGVYSLGMVTALG
                     GGSLRDILLNRHPLFWMEHYEYAILLLALSITSSLVAQEFFERKSMVHVVLALDALGL
                     GSFSASGASLADQQGCALFISSLLGVITGVFGGVMRDIVSNELPYVFQRTELYATCSF
                     IGAWTYLLVVRNSGNDIIAAIACIAVTFVMRMIALRYKIKLPI"
     gene            complement(1709..2947)
                     /locus_tag="VFK23_01980"
     CDS             complement(1709..2947)
                     /locus_tag="VFK23_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET7317882.1"
                     /translation="MEQINLPLSHEQAIAKQKACNICHAGCLDCHYAPSREQGSHAMS
                     RVPPSANCTGGGRSTFVCHAGTMERRRGDSYLGRDFSEPPDLPEDVHVAKKVECVDCH
                     QTGPGGMGHIDRKATCQDCHIEVEQAMASGLHRNMVCEACHVNVLGGYEMTSWGPGMI
                     SSKPNPFKKYSLYYGPQGPPILIKDQHGRWMPVKIWPNSAGGIKETIAPRPGLTFRWP
                     KGETHDAYALLGTFSFPGGNNNYLAWIQVEQVAHPLGKSRSCKSCHGSESQEAAVIWR
                     YFDSQGAEPFTGSQKVVAGRSGLHVKDIKATSKIVPMEGGKTEYFAAWLHLGDIWKTE
                     GDYSIPKSDPVKYRGLDEGVRDALARLELIDRKLKAREATGEDTKKLRRRWKEARAVA
                     VHEPGGAVKMIEELAGMVHY"
BASE COUNT          612 a          930 c          956 g          679 t
ORIGIN      
        1 gttattccag ggatgaagtc gtcggcagat cgcacatggc tatgttccac ggtgccgcca
       61 acggggtctc ttgtcctttt attgagcatg ccctcgttaa gcaggaacgg atcatcgccg
      121 aggaggtcgc ggcgaggaaa aaaagcggcg aagacgtcac ggtcgtcgtg acgggctccc
      181 cgatctatga cgatgccgga gagcttgcgg gggccgtcga gattttcagg gacgttacgg
      241 acatcaagag gagggaacgg gagcggaaga atatcctttc gatgttcgcc cacgacatga
      301 aaaacccggt taccgtgtcc gggggatttt tagcgcgcct gctttcggac aaggccgggg
      361 cgctcacgga aaaacagaga caatacctcg aaacgatacg ggaggagttg tataaagtgt
      421 ccgacctgct ggcggatttt ctcgaattct cgaggatcga ggcccgtgca tacataccgg
      481 tcttccgtcc gttcgatatc gaggagacgg tgcgccggaa catcgaggcc tcgaaagtgg
      541 ccgcggacag gaagaacata gccgtctccc ttgaagtgac cggggaggtt tcttcatcaa
      601 tccttgcgga cccgtcgatg atagaccgcg tcattgcgaa cctgcttgac aatgcgatag
      661 catacacgga cgcgggcgga atcgtccgcg tcaggatctc cggaagggag ggcgcggtcc
      721 tcgtttccgt cgaggatacg gggcgaggca tctcgaagga tcagattccc tatctgttcg
      781 atgcgttcta tcgcgccagc cgggactcca agggctccgg actcgggctg ttcatcgcta
      841 aaacgaacat cgagtcgcac ggaggccgaa tatgggtgga gagcacgccc ggcaagggca
      901 gcacgttcag cttcaccctg ctcagacgat aacggcaggt gagccggtgc ccaggtccta
      961 ccctcagatc ggcagcttaa ttttatacct gagcgcgatc atacgcatga cgaatgtcac
     1021 ggcgatgcag gcaatcgccg caatgatatc gttgccggag ttcctgacga cgaggagata
     1081 ggtccaggcc ccgatgaagg agcaggttgc gtagagttcc gttctctgga acacgtaggg
     1141 gagttcgttg ctgacgatgt cgcgcatcac gccgccgaag acgcccgtga tcactcccaa
     1201 gagcgacgag atgaacaggg cgcagccctg ctggtcggcc agggacgcgc ccgaggcgct
     1261 gaaggacccg aggccgagtg cgtccagggc caggacgaca tgcaccatgc tctttcgttc
     1321 gaagaactcc tgggctacaa ggctggacgt gatcgaaagg gccaggagca ggattgcata
     1381 ttcgtagtgc tccatccaga acaggggatg gcggttgagg aggatgtccc ggagcgagcc
     1441 gccgcccagg gccgtgacca tgccgagcga gtagacgccg atgaggtcca tccccttctt
     1501 cctggccgcg tagaatcccg tcagggcgaa ggcgatgatg cccaggactt ctattgttct
     1561 gatgagggtg ttcataaggc tgtcttttta cctgtactac agaggttttt gtcattcccg
     1621 cggacgcggg aatccatttt gaagtttact tataagagcc aatggatacc tgcttgcgca
     1681 ggtatgacaa ggtacattac gaatattttc agtagtgcac catccccgca agctcttcaa
     1741 tcatcttcac cgcgcccccc ggctcgtgaa cagcaaccgc tcttgcctct ttccacctgc
     1801 gcctgagttt tttcgtatcc tcgcccgttg cctcgcgggc cttgagcttc ctgtcgatca
     1861 gttcgagccg ggcaagggca tctcgaacgc cttcatcgag gccgcggtat ttcacgggat
     1921 cggatttggg gatcgaataa tctccctccg tcttccagat gtctcccagg tgcagccacg
     1981 cggcaaagta ctccgtcttt ccgccttcca tgggaacgat cttcgacgtt gccttgatgt
     2041 cctttacgtg taggccgctc cttcccgcta cgaccttctg gcttcccgta aagggctccg
     2101 ccccctggga atcgaaatac cgccagatca ccgccgcttc ctgcgactcc gaaccgtggc
     2161 agctcttgca ggagcgggac ttgccgagcg ggtgcgctac ctgctcgacc tggatccatg
     2221 cgagatagtt gttgttccca ccgggaaagc tgaaggtccc caaaagggcg taggcgtcgt
     2281 gcgtctcgcc cttgggccag cggaaggtga gcccgggcct gggtgcgatc gtttccttga
     2341 tcccgcccgc gctgttcggc catatcttca ccggcatcca gcggccatgc tggtctttta
     2401 tcaggatcgg cggcccctgg ggcccgtaat agagcgagta cttcttgaaa gggttgggct
     2461 tggatgagat catgcccggc ccccaggacg tcatctcgta tccgccaagg acattgacat
     2521 ggcaggcctc gcataccatg ttccggtgca gccccgaggc catggcttgc tcgacctcga
     2581 tatgacagtc ctggcacgtc gcctttctgt cgatatgccc catgccgccc ggcccggtct
     2641 ggtggcagtc aacgcattca accttctttg ccacgtgcac atcttccggc aggtccggcg
     2701 gctcggagaa atccctgccg agatagctgt cccctctcct ccgctccatc gtgcccgcgt
     2761 ggcagacgaa cgtgctcctg ccgccgcccg tgcagttggc cgagggagga acgcggctca
     2821 tggcgtggct gccctgctcc cgcgagggtg catagtggca gtcgaggcat cccgcgtgac
     2881 agatgttgca cgccttctgc tttgcgatcg cctgctcatg ggagagcggg aggttgatct
     2941 gttccactat cctcttggtg ttctcgaagg agaacctgtc tccctcagcc ctgccgaccg
     3001 gccgggtgtc cgcgaaagaa ggccctcagt tgttcgggcc gtgaatgtcg ttccagacct
     3061 gcatggagcg ttgcctgaag ttcgagccca tgacgctcgt ggaaaattgt ttgacctctt
     3121 cgggatggca ggaggacttt ccgcaggtct tctttgccag ggcagggtcg tatccca
//
LOCUS       DASSLD010000106         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1625285, whole genome shotgun sequence.
ACCESSION   DASSLD010000106 DASSLD010000000
VERSION     DASSLD010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625285"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFK23_01985"
     CDS             <1..276
                     /locus_tag="VFK23_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET7317883.1"
                     /translation="IRKDMNECIRGIVDGLPDGYRTVLVLSEFEGFTNAEIAKVAGIS
                     LDAVKIRLHRARTRLRKELESKCSFYHDKRNELACDRKTIPLKFPVK"
     gene            808..1380
                     /locus_tag="VFK23_01990"
     CDS             808..1380
                     /locus_tag="VFK23_01990"
                     /inference="COORDINATES: protein motif:HMM:NF020434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HET7317884.1"
                     /translation="MKESNTSFTLEISKTDKTCPVGEKIGSRNIEEGKIPVLSCEGAC
                     IRGEIARLTANLVAKQEPYRRGCHGELFTVPPSALAQWVKKAGKIVLIDGCFLRCHGR
                     ILENLVGKENLVQFDALSFYGKYTDRFDIDSVPEAERKETARRVADLVLSRLNNVTGR
                     DADFRAEAVCGAGASGASAAAATNQNRGCC"
     gene            1393..>2321
                     /locus_tag="VFK23_01995"
     CDS             1393..>2321
                     /locus_tag="VFK23_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7317885.1"
                     /translation="MNIPYVALKNLRRKPTRTLLLVSIVAAVSCTLFAATLFLNSVNN
                     ALVRGAYRLGADILVVPGNAEGKTRSALLSGEPTQFLMERSILDRIRSTDGVKAVTPQ
                     IFIKPASFFCCLTEDVFLVAFDPETDFTIKPWLEKKVGRKLSVNEVIIGGRIPVIVGD
                     TLPFFGTHFIVAGILEPTGMNFVDRTVFMSMEAAYAMAENSKVRAVQPLEIGRDRIST
                     VLVKVKDDMSPDRVALWIERDIPGVKALVSDTVISTVRKQLSGLIRAIFGISGTLWLI
                     VLLIMAFAFYMIVNERRREIGLLRAMGANKGHIS"
BASE COUNT          561 a          563 c          678 g          519 t
ORIGIN      
        1 atccggaagg acatgaatga gtgtattcgc ggcattgtgg atggcttgcc ggacggatac
       61 cggactgtgc tggttctcag cgaatttgaa ggttttacca atgccgagat cgccaaggtt
      121 gccggcatat ccctggacgc ggtaaagatc agactgcatc gggccaggac gagactcagg
      181 aaagaactgg aatcgaaatg cagtttctac cacgataagc gcaatgagct tgcctgcgac
      241 cggaagacca tccccttgaa atttcccgta aaatagaggc ttccttttct acgactttct
      301 tcctatcatt taaataaggg tgtacccttt ttctcctttc cttcgtctat acggtcaaaa
      361 ggaggtgatg cctatgtttc cctgcgcatt ttccggttac ggattttggt ggatttgtcc
      421 ggtcatcatg attggcatga tggtcctctg cttctttatg atgagaggac gtatcggctc
      481 gatgatgtgc cggccaggtt cccgcagttc aggcggctgc ggaggagaga tttctgagac
      541 agaccgggaa ccacaacggt aaagagacta aaggaggcct gaaatgggtg ctgaagagac
      601 aaacgagacg gacgacaaag caaaatccgc aaactccgga tgtggtccga tgagtcatga
      661 aatgtttgag acgatgagtg ggtgctgcac gggaccgggc ggttcccctg actgctcaac
      721 catgatgaaa agcatgatgg aaacaatgaa aagctattcc tgctgttcac ctggaaaagg
      781 ggacgccggg tctgagagga gggaaaaatg aaagagtcga ataccagctt cacccttgag
      841 atttcaaaaa ccgataaaac ctgtcctgtg ggagaaaaga tcgggagcag aaatattgaa
      901 gaaggtaaaa tcccggtcct gtcctgcgaa ggggcctgca tccggggaga aatcgcccgt
      961 ctcaccgcaa acctggttgc gaagcaggag ccgtaccgga ggggttgcca cggggaattg
     1021 tttaccgtgc ctccatcggc attggcgcag tgggtcaaga aagcgggaaa aatcgtgctc
     1081 atcgacggct gtttccttcg atgtcacggc aggattctgg aaaatctggt cgggaaagag
     1141 aacctggtcc agttcgatgc cctttctttt tatgggaaat atacggaccg tttcgatatt
     1201 gactccgtcc cggaagcgga acgcaaagag acggcgcgca gagtggcgga ccttgtcctg
     1261 tcacgcttga ataatgtgac agggcgcgac gcagattttc gggctgaagc ggtatgcggg
     1321 gcaggtgcat ccggagcttc tgcggctgca gcaacaaatc agaatcgcgg ttgttgctga
     1381 atcgaggtgc ctatgaacat tccttatgtt gcgctcaaaa acctcaggag aaagccgacg
     1441 agaacgctgc tcctggtctc catcgtggct gcggtgtcgt gcaccctttt cgcggccact
     1501 ctcttcttga atagcgtaaa caatgcgctt gtaaggggcg cataccggct tggggctgac
     1561 atcctcgtag tgcccgggaa cgcggagggg aaaacacggt cggcgctcct ttcaggcgag
     1621 ccgacgcaat tcctcatgga gagatcgatt cttgaccgga taagaagcac ggatggcgtg
     1681 aaggccgtta cccctcagat attcatcaag cctgcgagct tcttctgctg cctcacggaa
     1741 gacgtatttc tcgttgcctt cgatcccgag acagacttca ccataaaacc ctggctcgaa
     1801 aagaaggtcg gcaggaagct ctcggtcaac gaggtcatca tcggcggaag aataccggtc
     1861 atcgtgggcg atacgcttcc ctttttcggc acgcatttca tagtcgccgg catcctcgag
     1921 cccacgggta tgaactttgt tgaccgaacc gtgttcatga gcatggaggc agcgtatgcc
     1981 atggcggaga actcaaaggt ccgggcagtc cagccgctgg agatcggaag ggacaggata
     2041 tcgaccgtcc ttgtgaaggt aaaagacgat atgagtccgg accgggttgc gttatggatc
     2101 gagcgtgaca taccgggagt caaagccctg gtttcggata cggtcatcag cacggtgaga
     2161 aagcagctct cagggctgat cagggccatt tttggcatca gcggcactct ctggcttatc
     2221 gtccttttga tcatggcatt cgcattctat atgatcgtga acgagcggcg cagggagatc
     2281 ggactcctgc gggccatggg cgcaaacaag ggccatatat c
//
LOCUS       DASSLD010000107         2955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_608637,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000107 DASSLD010000000
VERSION     DASSLD010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608637"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /locus_tag="VFK23_02000"
     CDS             <1..1362
                     /locus_tag="VFK23_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7317886.1"
                     /translation="LVVDDEQSLRDFLAIMLKKEGHDVVTAENGTHALNAVQSEIFDL
                     VISDVKMPGIDGISVLKSVKEISPETVVIMITAYATAETAVEAMKLGASDYIIKPFKV
                     DELKLTINNSLEKRHLRKENILLKREIKSRAGFENFIGKSEAMQKVFTLIRQVAETNS
                     TVLITGESGTGKELVARAVHFNSPRKDGPFVTVNCGALPETLLESELFGYMKGAFTGA
                     ASNKQGLFEAADGGTMFLDEISATTPALQIKLLRVLQEREFMRVGGTAGIKVDVHIIA
                     ASNKELRMEVSKGAFREDLFYRLNVIPIHIPPLRERKEDIPPLVEFFLKKISRKEGDR
                     VAVKKMEADAMKALMAYHWPGNVRELENMIERLVIIAPGDSIGLEHIPGGARNMAPCA
                     DLGQLDIPDEGLDLEAVLGNAEKTLLKRALEKSGGVKTEAAKLLKLSFRSFRHRLQKF
                     DAT"
     gene            1577..2551
                     /locus_tag="VFK23_02005"
     CDS             1577..2551
                     /locus_tag="VFK23_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET7317887.1"
                     /translation="MKTTAASLIIHNEHSLPSLYGDLDKNLRLIEHTYGVNVHARGNR
                     IQIEGDEKSVASVERLIIQLADMLAEGVITDKDDVNHAIQAFSADPAAALKDIFQKTI
                     PVSSRKRPVSPKNETQRTYVDAIRRHDVVFGIGPAGTGKTYLAMAMAVSALLRREVSR
                     IILVRPAVEAGEKLGFLPGDLYEKVNPYLRPLYDALYDMIETEKANKLVERGDIEIAP
                     LAFMRGRTLNDSFIILDEAQNTTSEQMKMFLTRLGFNSKTVITGDVTQIDLPNGRASG
                     LIEVQAILDGVEGIRFIHFTNRDVVRHKLVQQIVKAYERYEAANEQQK"
     gene            2535..>2955
                     /locus_tag="VFK23_02010"
     CDS             2535..>2955
                     /locus_tag="VFK23_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317888.1"
                     /translation="MNSKNKTDVKLLRRNMAQNSGREEQDEGGVPRPARFANSSIAVA
                     VAVLTAFVLQGTGDPLSVYVGLAALLLVFFYLFSRDVKRYRPALLGDTKKILLLALLL
                     FATVALSQLAVRFSTHLAQEFHLDGKTVAFALPVSAGA"
BASE COUNT          697 a          838 c          872 g          548 t
ORIGIN      
        1 ctcgtcgtcg acgatgaaca gagcttgagg gattttctcg ccatcatgct caagaaggag
       61 ggccacgacg tcgtcacggc ggaaaacggc acgcacgcgt tgaacgcggt acagtcggaa
      121 atattcgacc tcgtcatcag cgacgtgaaa atgccgggca tcgacggcat cagcgtcctc
      181 aagagcgtca aggagatctc tccggaaacc gtcgtcatca tgattacggc atacgcgacc
      241 gcggagaccg ccgtcgaagc gatgaaactc ggagcgtcgg attacatcat aaagcccttc
      301 aaggtcgacg agttgaagct gaccatcaac aattcgctcg agaaacggca cttgagaaaa
      361 gagaacatcc tgctcaaacg ggaaatcaag tcgagggccg gctttgaaaa cttcatcggg
      421 aagagcgaag ccatgcagaa ggtctttacc ctcatccggc aggtagcgga gaccaacagc
      481 accgtcctga tcaccggtga aagcgggacg ggcaaggaac tcgttgcgag ggcagttcat
      541 ttcaacagcc cgaggaaaga cggtcccttc gtgaccgtca attgcggggc gctgcccgag
      601 acgctgctcg aaagcgagct gttcggatac atgaagggcg cattcaccgg cgcggcgtcg
      661 aacaagcagg ggctcttcga ggccgccgac gggggtacca tgttcctcga tgagatcagc
      721 gctacgacgc cggcactgca gataaagctg ctgcgtgttc tgcaggagcg ggagttcatg
      781 cgtgtcggcg gaaccgcggg gatcaaggtg gacgtgcaca tcatcgccgc gagcaacaag
      841 gaactgcgga tggaagtctc aaaaggggcg ttccgagaag acctctttta ccggctcaac
      901 gtcattccca tccatattcc tccgctcagg gagcggaagg aggatatccc gccgctcgtc
      961 gagtttttcc tcaagaagat atcccggaag gaaggcgacc gggttgcggt gaagaagatg
     1021 gaggcggacg ccatgaaagc gctcatggcg tatcattggc cgggcaacgt ccgggaactg
     1081 gaaaacatga tcgagcgcct cgtcatcatc gcccccgggg actcgatcgg actggagcac
     1141 ataccgggag gcgcgagaaa catggcgccc tgcgccgatc tcggacaact ggacattccc
     1201 gacgagggcc tggacctcga ggccgtgttg ggaaacgccg aaaaaacgct gctcaagagg
     1261 gcgttggaaa agtccggcgg ggtgaagacc gaagccgcga agctcctgaa actatccttc
     1321 cggtcgttca ggcaccgtct gcagaagttc gatgcgacct gaccgcgcgc ccggagggtg
     1381 cacgggacaa agtgaaattg tgaagctccc ggggagcaag ctggggtcat cgattcattc
     1441 ggtgagataa ttatctgatc cgcccgctac gccatttgaa ggttcgtaga ggtatctcca
     1501 gaattgactt tacccccgcg ccatgctaac atgtgatatg aaaatacgat catgaacgaa
     1561 tgatcgtagc catgcaatga agacaacggc ggcaagtctc atcatacaca acgaacactc
     1621 gttgccgagt ctctacggcg acctggataa aaatctccgg ctgatcgagc atacatacgg
     1681 ggtcaatgtg catgcacggg gaaaccgcat ccagatcgaa ggggacgaaa aatcggtggc
     1741 cagcgtcgag cggcttatta tccagctcgc cgatatgctg gccgagggcg tcattaccga
     1801 caaagacgac gtaaaccacg ccattcaagc cttttccgcg gaccctgcag cggccttgaa
     1861 ggacattttc cagaagacaa tacccgtatc cagcaggaaa cggcccgtat cgccgaaaaa
     1921 cgaaacccag cgcacgtacg tagacgcgat caggcggcac gacgtcgtgt tcgggatcgg
     1981 cccggccggc acggggaaaa cgtacctggc catggccatg gcggtctcgg cgctcctccg
     2041 gcgcgaggtg agcaggatca tcctcgtgcg gcctgcggtc gaggccggcg agaaactcgg
     2101 gttccttccc ggagacctgt acgaaaaggt caatccctat ctgcggccgc tctacgacgc
     2161 gctctacgat atgatcgaaa cggaaaaggc gaacaagctt gtggagcggg gagacatcga
     2221 gatcgcgccc ctcgcattca tgcgcggacg gaccctgaac gattccttta ttatcctcga
     2281 cgaggcgcag aacaccacgt ccgagcagat gaagatgttt ctcacgcggc tcggcttcaa
     2341 ctcgaagacc gttattaccg gtgatgtgac tcaaatagac ctgccgaacg gtcgggcttc
     2401 gggcctcatc gaggtgcagg cgatcctgga tggcgtcgag ggcatccgtt tcattcattt
     2461 taccaaccgg gacgtggttc gtcacaagct cgtccagcag atcgtcaagg cttacgagcg
     2521 ctacgaggcg gcaaatgaac agcaaaaata agacggacgt gaagctcctg cgccggaaca
     2581 tggcgcagaa ctccggccgt gaagagcagg atgagggagg tgttccgcgc cccgccaggt
     2641 tcgccaatag cagcatcgcc gtcgccgtcg cagtcctcac ggcattcgtc cttcaaggaa
     2701 ccggagaccc gctcagcgtc tatgttggtc ttgccgccct cctgctggtc tttttttatc
     2761 ttttctcccg tgacgtcaaa cggtatcgtc ctgcgctcct gggagatacg aaaaagatcc
     2821 tcctgctcgc gctcctgttg tttgctaccg ttgccctgtc gcaacttgcc gtacggttct
     2881 cgacgcacct ggctcaagag ttccatctgg acgggaaaac cgtcgcgttt gctcttccgg
     2941 tgtccgccgg tgcaa
//
LOCUS       DASSLD010000108         3729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_543254,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000108 DASSLD010000000
VERSION     DASSLD010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543254"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VFK23_02015"
     CDS             <1..245
                     /locus_tag="VFK23_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HET7317889.1"
                     /translation="MAGLFATVAVNAAGANGAFFGNPRQFFVQAGAVSLVALYSFVAS
                     FVMLKVLDAAMGLRIESEQETEGLDISQHGEAGYSL"
     gene            complement(283..1152)
                     /locus_tag="VFK23_02020"
     CDS             complement(283..1152)
                     /locus_tag="VFK23_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7317890.1"
                     /translation="MFDFRLLFVAVVWGVNFSAVKLALADFHPLAFTVIRFGLAAVFL
                     VVVMLAGREPLAIERQDRFAVARLGFVGIALFNVFFMYGLKYTTAANSALLVSLSPLF
                     GALFGIVWGHESLTARAGAGLALSTAGVILIIRSHYGEMSFSAAGIAGDLVTLGAPVT
                     WAIYTAAAKPLLEKYSAVKITAYSMLAGCAPLLPLSLPGLLGQSWAALPLRAWLLMAF
                     SAFIAGGAAYALWYRGLKQRGVTRTIVYHYVTPFAAILFAALATGEKIQALQVAGGAA
                     ILVGVYLVQQQTR"
     gene            1293..2411
                     /locus_tag="VFK23_02025"
     CDS             1293..2411
                     /locus_tag="VFK23_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7317891.1"
                     /translation="MEASSYRIEDIAADILSGIIDGVVVTDVEGRVLIWNRAAEELTG
                     IAAADALGKNLRDVLADNPSVVGQVEKTLASGRSYSEYEAEIAVKYGPHHPVGLVTSM
                     LTDDRGKPTGAILTIRDQTGVRDLKERMRRSDRLATLGMIAAGIAHEIKNPLVGIRGA
                     AQLMKAEIRSGSREAPAAGKNMTEYLDVILKESDRLNKVLEGILDFTRLKPREAKSSN
                     IHSILDRVLLLNEDSARRRGIVLTRLYDPSLPEVYGSEDQLIQVFLNITRNAVEAMPK
                     GGKLTVMTRMSDLFTTVQSDGRKYQLMVVKVSDTGAGIKQEHLQDIFTPFFTTKDRGV
                     GLGLALSYQIVQEHLGTIRVESQENEGTTFSVYLPLTA"
     gene            2572..>3729
                     /locus_tag="VFK23_02030"
     CDS             2572..>3729
                     /locus_tag="VFK23_02030"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7317892.1"
                     /translation="MTTERVLIADDDESVRWVLKKALEKEGIETALAKDGNEALERLK
                     EGDISIVLMDIRMPGMGGLEALDKIRSAGDGPAVIIMTAQATMQNAIEAMRRGAFDYI
                     TKPFDLDEVNILIRKALEIRHLSQEVNALRAEVREKYEGGLVGNTAAMQEIYKTIGRV
                     AESDATVLIHGESGTGKELVARAIHYHSKRAGRPFVAVNSAAIPSELLESELFGHEKG
                     SFTGAVARKIGKFEAASGGTLFLDEIGDMNLPLQGKLLRVLQEKEFERVGGTEPIKTD
                     VRVLAATHHNLEKMVREKRFREDLFYRLNVIQINIPPLRKRKDDIVALAEFFMQKYQG
                     SDSKKKILTPETLKILRAYDWPGNVRELENAVQRAITLSQGDKIFPDSLPPQ"
BASE COUNT          824 a         1061 c         1217 g          627 t
ORIGIN      
        1 ttatggccgg attgttcgcg accgttgcgg tcaacgccgc gggcgcaaac ggtgccttct
       61 ttggcaaccc caggcagttc ttcgttcagg ccggagccgt gtccctggta gccctctact
      121 cgttcgttgc aagcttcgtg atgctgaagg tgcttgatgc ggcgatgggc ctcaggatag
      181 agtccgaaca ggagacggaa gggctcgaca tcagccagca cggagaggcg gggtattcat
      241 tgtagaggca aagcatcgac aggccgaggg ggagacgggg cgtcatctcg tttgttgctg
      301 aacaagataa acgccgacga ggatggccgc tccgccagca acctggaggg cttggatttt
      361 ctcgccggtt gcgagggccg cgaagagtat cgccgcgaag ggcgtgacat aatggtacac
      421 gatggtccgg gtgacgcctc tctgcttcaa accccggtac cacagggcat aggcagcgcc
      481 ccctgcgatg aaggcggaga acgccatgag taaccatgcc cggaggggca gtgcggccca
      541 gctctggccg agcaggcccg gcaggctcag gggcagaagc ggggcgcatc ccgcgagcat
      601 gctgtaggcg gtgattttga cggcggaata tttttcgagc agaggcttcg cggcggcggt
      661 gtagatcgcc caggtgaccg gcgcgccgag ggtcacaaga tcccccgcga ttcctgcggc
      721 ggagaagctc atttctccgt agtggctcct gatgatgagg atgacgccgg cggtcgagag
      781 cgcaagaccc gcaccggcgc gcgccgtgag gctttcatgt ccccatacga tgccgaacag
      841 cgctccgaac agcggcgaca gggacacgag aagcgccgaa ttcgctgcgg ttgtgtactt
      901 gaggccgtac atgaagaaga cgttgaacag ggcgatgccg acgaacccca gcctcgcgac
      961 ggcgaaacgg tcctgccgtt cgatggcgag cggctcgcgc ccggcaagca tcaccacgac
     1021 aagaaaaact gcggcaaggc cgaaccggat cacggtgaag gccagcgggt gaaagtcagc
     1081 gagcgctaac ttgacggccg agaagttgac gccccagacg actgcgacga agaggagacg
     1141 gaagtcgaac atgatcgtaa tgcggaatgg gggaacgtgg agtagaaaat cggaaagagc
     1201 gctgcctatt gacgttgccc gtcactccga actcgactcc actataccgg aaagaacttg
     1261 aatactcaaa tatattgtgt tatcaatgaa gcatggaggc atcaagttac cgcatagagg
     1321 acatcgccgc agacatcctg agcgggatca tcgacggcgt ggtggtcacc gacgttgagg
     1381 gccgcgtgct catatggaac cgggcggccg aggaactgac ggggattgcc gccgccgatg
     1441 ccctcgggaa aaacctgcgg gacgttttgg cggacaatcc gtccgtggtc ggccaggttg
     1501 agaaaaccct ggcctcggga aggtcctatt cggaatacga ggccgagatc gcggtaaaat
     1561 acggtccgca tcaccccgtc ggcctggtca cgagcatgtt gacggacgac cggggtaagc
     1621 ccacgggcgc gattctcacc atccgggacc agacgggtgt ccgggacctg aaggagcgca
     1681 tgcggaggtc ggaccgtctg gccacgcttg gaatgatcgc ggccggcatc gcgcacgaga
     1741 taaagaaccc gctggtcggt atccgcggtg ccgcgcagct catgaaggcg gaaatccggt
     1801 ccggaagccg ggaagccccg gccgccggca agaacatgac cgagtacctc gacgtgatcc
     1861 tgaaggaatc ggaccgcctc aacaaggtgc tggaagggat cctcgatttc acgaggctca
     1921 aaccccggga agcgaagagc tcgaacatcc acagcatcct ggaccgcgtg ctgctcctga
     1981 acgaggacag cgccaggcgt cggggcatcg tgctcacccg gctctacgac ccgagcctgc
     2041 ctgaggtcta cggcagcgag gaccagctca ttcaggtgtt tttgaacatc accaggaacg
     2101 ctgtcgaggc aatgccgaag ggcggaaagc tgacggtcat gacgcgtatg tccgacctat
     2161 tcacgaccgt gcagtccgac ggccggaaat accagctcat ggtggtgaag gtgagcgaca
     2221 ctggcgcggg gatcaagcag gaacatcttc aggacatctt cactcccttc ttcacgacga
     2281 aggaccgggg ggtgggcctg ggtctggcgc tctcgtatca gatcgtgcag gagcatttgg
     2341 gaacgatccg ggtcgaaagc caggagaatg agggaacgac cttcagcgtg tatctgccgc
     2401 tgacggcgtg attcaaatgc ggaatgcgaa attcggaatg cggaataaaa gactgtatcg
     2461 cctgagttct ttcgtctttt ttggtcattc cgcacaccgc attccgaatt ccgcatttgg
     2521 tcggatgcct cgtgatggtg gttgcaccgc agcattcttc gggaaactga aatgacaact
     2581 gagcgtgtat tgatagcaga cgacgacgag agcgtccggt gggtgctcaa gaaggccctc
     2641 gaaaaagagg gaatcgagac ggccctggcc aaggatggga acgaggcgct tgaaaggctc
     2701 aaggaagggg acatctcgat cgtgctgatg gacattcgca tgcccgggat gggcgggctt
     2761 gaggccctcg acaagatccg gtccgcgggc gacgggccgg cggtcatcat catgacggcc
     2821 caggccacca tgcagaacgc cattgaagcc atgcgccggg gggccttcga ttatatcacc
     2881 aagccctttg atctcgatga ggtgaacatc ctcatccgga aggctcttga aatcaggcat
     2941 ctctcgcagg aagtgaacgc gctccgggcc gaggtgcggg agaaatacga gggcgggctc
     3001 gtgggcaata cggccgccat gcaggagatc tacaagacca tcggccgcgt cgccgaaagc
     3061 gacgccacgg tcctgatcca cggcgagagc ggcacgggca aggaactcgt tgccagggct
     3121 attcactatc acagcaagcg ggcgggcagg ccgttcgtgg ccgtgaactc cgcggccatc
     3181 ccgtcggagc tcctcgagag cgagctcttc ggccacgaga agggctcctt taccggcgcg
     3241 gtcgcccgca agatcggaaa gttcgaggcg gcgtcgggag ggaccctgtt cctggacgag
     3301 atcggcgaca tgaacctgcc gctccagggg aagctcctgc gcgtgctgca ggaaaaggag
     3361 ttcgagcgcg tgggcggcac cgagccgatc aagaccgatg tgcgcgtgct cgccgcgacc
     3421 caccacaacc tcgaaaagat ggtgcgcgag aagcgttttc gcgaggacct gttctaccgc
     3481 ctcaatgtga tccagatcaa cattccgccg ctccgcaagc gcaaggacga catcgtcgcg
     3541 ctcgccgagt tcttcatgca gaaataccag ggcagcgact cgaagaagaa gatcctgacg
     3601 cccgagacgc tcaagatcct ccgcgcttat gactggccgg gaaacgtgcg cgagcttgag
     3661 aacgccgttc agcgcgccat caccctgtct cagggcgata agatatttcc cgactccctg
     3721 ccgccgcag
//
LOCUS       DASSLD010000109         2420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1423629, whole genome shotgun sequence.
ACCESSION   DASSLD010000109 DASSLD010000000
VERSION     DASSLD010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423629"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFK23_02035"
     CDS             <1..448
                     /locus_tag="VFK23_02035"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HET7317893.1"
                     /translation="PLLQMVAPDILDMDMPIEGVFHNNVIVKIRKRYAGHGKKVINTI
                     WGTGMLMLSKFVIVCDEDVNIHDYSEVTWKVMNHVDPQRDVVISDGPLDILDHSCPQI
                     GFGGKMGVDATRKGPGEGFLRPWPDEIRMDPQVQKRVEERWKEYGF"
     gene            591..1913
                     /locus_tag="VFK23_02040"
     CDS             591..1913
                     /locus_tag="VFK23_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET7317894.1"
                     /translation="MLLFDIILIFSLIILNGFFAASEISLISLRKSRVRHLVKSGHRV
                     ARRVQKLQEEPERFLSTVQIGVTLIGTLAAAVGGLIAAERLKPFFVAVPVPFIQKAAE
                     PLAIAIVTGLITYVTLIIGELVPKALAIRYSERIAFFMSAPIDFLSRALASILKVINL
                     STDCVLGALGFERGQEQTFISEDEVKYLIREGRKSGVFEPSEEDLIHSVFRFTDTLVR
                     EVMVPRTEIVAVEAGSDIDTILRTMNEKGFSRLPVYRETIDNIIGIVYLKDILTLHME
                     NRPLRLESALQKPYVVPPNKNVRVLLKEMREKRIHLALVGDEYGGTDGLVTIEDLIEE
                     IVGDIRDEQEKELKEIHEIAANRYVIDGRTDIGKVNAILGVQLPEDEFETMGGFVLGL
                     FGRLPSEGDQIRYNNLLFTVLRLRKNRISRVRVLKYAPEENQEPEKES"
     gene            2188..>2420
                     /locus_tag="VFK23_02045"
     CDS             2188..>2420
                     /locus_tag="VFK23_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET7317895.1"
                     /translation="MTTPLVLETNLEGLQPPRRGKVRDVYDLGDKLLIVATDRISAFD
                     VVLPNAVPEKGRVLTQISKYWFEQTEKIVKNHV"
BASE COUNT          581 a          640 c          695 g          504 t
ORIGIN      
        1 gccgctcctc cagatggtcg cgcccgacat cctggacatg gacatgccca tcgagggggt
       61 gttccacaac aacgtgatcg tgaagatcag aaaacggtat gcaggacacg gcaagaaggt
      121 gatcaacacg atctggggca caggcatgct catgctctcc aagttcgtca tcgtctgcga
      181 cgaggacgtg aacatccacg actattccga ggtgacgtgg aaggtcatga accacgtgga
      241 cccgcagcgc gacgtggtga tctccgacgg gccgctcgac atcctcgacc actcctgccc
      301 gcagatcggc ttcggcggca agatgggggt cgacgccacg cgcaaaggcc ccggggaagg
      361 cttcctgcgg ccgtggccgg acgagatcag gatggacccg caggttcaga agcgcgtgga
      421 agagcggtgg aaggagtacg ggttctagga aaagcaccaa atctcaaata tcaaatccca
      481 aataaatcaa attcaaatat cagattctag acgctggagt tgattttctt cggtcattgg
      541 agcttatttg agatttggaa tttgttattt ggagccttcc gttatcatct atgctcttat
      601 tcgacatcat cctgatattt tcgctgatca tcctgaacgg atttttcgcc gcgtccgaga
      661 tatcactcat ctcgctcaga aaaagcaggg tccgccacct cgtcaagagc ggccatcgcg
      721 tcgcgaggag ggtgcagaaa ctccaggagg agccggagcg cttcctcagc accgtccaga
      781 tcggcgtgac cctgatcggg acgctcgcgg ctgcggtagg cggcctcata gcggcggagc
      841 gcctgaagcc gttcttcgtc gcggttccgg tcccgttcat ccagaaagcc gcagagcccc
      901 tggccatcgc tatcgtgacc ggcctgatca cgtatgttac gctcatcata ggggaactcg
      961 tcccgaaggc gcttgcgatc cggtattcgg agcgtattgc cttttttatg tcggccccta
     1021 tcgattttct gtcgagggcg ctggcttcca tactgaaggt catcaacctc tcgaccgatt
     1081 gcgtgctggg cgctttaggg tttgagcgcg gccaggaaca gaccttcatt tccgaggacg
     1141 aggtgaagta tctcatccgg gaaggccgca agagcggcgt ttttgagccc tcggaggagg
     1201 acctgatcca cagtgtgttc cgtttcacgg acaccttggt gagggaagtc atggtgccgc
     1261 gcaccgagat cgtcgctgtt gaggcgggga gcgacatcga tacgatcctc cgcacaatga
     1321 acgaaaaggg cttttcccgt ctgccggtgt accgggagac catcgacaat atcatcggca
     1381 tcgtttacct caaggacatc ctgacgcttc atatggaaaa ccggccgctc aggctcgaaa
     1441 gcgcgctgca gaaaccttat gttgtcccgc cgaacaagaa cgtgagagtc cttctcaaag
     1501 agatgcgcga aaagcgcatc catctcgccc tggtgggaga tgaatacggc gggaccgacg
     1561 gcctggttac catagaggac ctgatcgagg aaatcgtcgg cgatatccgc gacgagcagg
     1621 aaaaggaact gaaggagatc cacgagatcg cggcgaaccg gtatgtcatt gacggcagga
     1681 cggacatcgg aaaagtgaat gcaatactgg gcgtgcagct ccccgaggac gaattcgaga
     1741 ccatgggcgg cttcgtgctg gggctcttcg gaagactgcc ttccgaagga gaccagatcc
     1801 ggtacaacaa ccttctgttt accgtgctcc ggctgcgaaa aaaccgcatc tcccgcgtcc
     1861 gggtgctgaa gtacgcgcct gaagaaaatc aggagcccga aaaagagagt tgaaaccacg
     1921 ggtcccgcga agacgcggga cacacagtta gtataaagaa agacaattga cgcgcaaagc
     1981 cgctaagtcg caaagaaaaa caaatcgtgt ttccggttta aattcaagat tccaaagccg
     2041 ttgattctct ttgcgtgctg tgcgactttg cgcgagatag acctatgcat tacaatttgt
     2101 tttatgattt aaatccgtgt tcatcggtgc gttccgtcga ggacggaacc cgtggttaca
     2161 ttttttttag atgatgaaga ggtgaaaatg acgacaccgc tggtacttga gacaaatctc
     2221 gaaggactgc agcctccccg ccggggaaaa gttcgtgacg tctacgatct tggggacaaa
     2281 ctgctcatcg tcgcgacgga caggatatcg gccttcgacg tggtcctgcc gaacgccgtc
     2341 ccggagaagg ggagagtcct gacccagata tcgaaatact ggttcgagca gactgaaaag
     2401 atcgtaaaga accatgtgat
//
LOCUS       DASSLD010000110         3341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_679877,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000110 DASSLD010000000
VERSION     DASSLD010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679877"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..737)
                     /gene="rpsD"
                     /locus_tag="VFK23_02050"
     CDS             complement(135..737)
                     /gene="rpsD"
                     /locus_tag="VFK23_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET7317896.1"
                     /translation="MARYIGSVCKLCRREGMKLFLKAERCYTDKCGVTRRSYPPGQHG
                     QARIKQSEYSLQLREKQKIRRVYGVLERQFRGYFEKAERMKGVTGDNLLQLLERRLDN
                     VVQRMGFAGAKKEARQLVRHGHLLVNGRKVNIPSYLLKAGDVVELREKSRGIAQIQQT
                     LAAVEKRGFPSWLEIDKAQFKGKVLSLPARDESTLPTVKE"
     gene            complement(810..1184)
                     /gene="rpsK"
                     /locus_tag="VFK23_02055"
     CDS             complement(810..1184)
                     /gene="rpsK"
                     /locus_tag="VFK23_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET7317897.1"
                     /translation="MAKKGKERKNVANGVAHIHASFNNTIVTITDTSGNVITWSSSGN
                     KGFKGSRKSTPFAAQLAAEAAAKKAMEHGMKQVEVYVKGPGTGRESAIRSIQAAGLEI
                     VAIKDVTPIPHNGCRPPKRRRV"
     gene            complement(1270..1662)
                     /gene="rpsM"
                     /locus_tag="VFK23_02060"
     CDS             complement(1270..1662)
                     /gene="rpsM"
                     /locus_tag="VFK23_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET7317898.1"
                     /translation="MARIAGVDLPKDKKIEIALTYIYGIGPTSARKILAEAGVDPNSK
                     SGNLTESDIVKIRAILDRSYTVEGDLRRDVTMHIKRLMDIGSYRGLRHRKGLPARGQR
                     TKTNARTRKGPKKSAMAGRGKKKGMAKK"
     gene            complement(1757..1870)
                     /gene="rpmJ"
                     /locus_tag="VFK23_02065"
     CDS             complement(1757..1870)
                     /gene="rpmJ"
                     /locus_tag="VFK23_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET7317899.1"
                     /translation="MKVRSSVRKICPKCSIIRRKGIVRVICENARHKQRQG"
     gene            complement(1932..2150)
                     /gene="infA"
                     /locus_tag="VFK23_02070"
     CDS             complement(1932..2150)
                     /gene="infA"
                     /locus_tag="VFK23_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET7317900.1"
                     /translation="MAKEDSIEVQGTVLETLPNAMFRVEMENGHKILAHISGKMRMHF
                     IKILPGDKVTVELTPYDLTRGRITYRFK"
     gene            complement(2207..2956)
                     /gene="map"
                     /locus_tag="VFK23_02075"
     CDS             complement(2207..2956)
                     /gene="map"
                     /locus_tag="VFK23_02075"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET7317901.1"
                     /translation="MIILKSLQEIEKIRQACLVVADVLDNIRALVQPGVSTQALDEFA
                     EQRILAAGAKPAFKGYRGYPKTLCTSVNYEVIHGIPSENVVLKQGDIVSIDVGAIVEG
                     FYGDAAITLPVGTIAPAAERLIRVTEESLYRGIDQARAGNRLYDISHAVQHHVEAHGY
                     SVVREFVGHGIGRSLHEDPQIPNFGPQGQGPRLKPGMVLAVEPMVNRGASATIVKEDG
                     WTAVTADGSLSAHFEHTVAVMPDGPWILTKR"
     gene            complement(2971..>3341)
                     /locus_tag="VFK23_02080"
     CDS             complement(2971..>3341)
                     /locus_tag="VFK23_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HET7317902.1"
                     /translation="YVLMIIFFAYFYTAIVFNPVDIADNLKKYGGFIPGIRPGKKTSD
                     YLFRVLNRITLGGALYLSAVCVLPDIFYKFFNVPFYFGGTSLLIVIGVALDTVSQIES
                     HLIQRHYGGFLKGVSIKGRR"
BASE COUNT          666 a         1042 c          812 g          821 t
ORIGIN      
        1 cctccagaac ggagtaaaga aacttttcac acgtaaagac cagggagcgt ccgtcaaagc
       61 ggacgcgcac cctgccctcg gcattattcg caatgtgttg aaggggctta cttcgaatag
      121 agctcgacga caagctattc ttttaccgtc ggcaacgtac tctcgtccct ggccggcaag
      181 gacaatacct ttcccttgaa ctgggccttg tctatctcca gccacgaggg aaatccacgt
      241 ttctcaacgg cggccagggt ctgctgaatc tgcgcaatgc ccctgctctt ctcccggagc
      301 tccacgacgt ctccggcctt cagaagatag gaagggatat ttacttttct gccgttgacg
      361 agcagatgtc cgtgccgcac cagttgacgg gcctcctttt tcgccccggc aaatcccatc
      421 cgctggacga cgttgtcgag cctgcgttcg aggagctgca ataaattatc gccggtcacc
      481 cccttcatcc gttcggcctt ttcaaaatag ccgcggaact gccgctcgag tactccatag
      541 acgcgccgga ttttctgttt ctccctgagc tgcaggctgt attctgactg tttgatcctg
      601 gcctgaccgt gctgtcccgg ggggtagctc ctccgcgtta cgccgcactt atccgtataa
      661 caacgctccg ccttcaaaaa gagcttcatg ccttcccgac ggcaaagctt acagaccgat
      721 ccgatatacc gtgccactga tacctcctgt gttaaacaaa ccgacccgtc agactcgtcg
      781 gacgagtcag acaagtccga atttctccgt tacacccttc tccgcttcgg cggcctgcac
      841 ccgttgtgag gaatcggcgt cacatccttg atcgccacaa tttcgagtcc ggcggcctgg
      901 atcgaccgga tggccgactc tctgcccgtc cccggtcctt taacatagac ctcgacctgt
      961 ttcatgccgt gctccatggc tttcttcgca gcggcttccg cggccaactg cgcagcgaag
     1021 ggggtgctct ttcgtgagcc cttgaatccc ttgttgccgg agctcgacca cgtgatgaca
     1081 ttgcccgacg tatccgtgat cgtcacgatc gtattattaa acgatgcatg gatatgcgcc
     1141 actccattgg caacattttt tctttctttt cccttcttcg ccattgctcc tcctcaaaaa
     1201 gcagttcaca gcctgtggct cttggcggca atctatggct ttgaaccttg cgctatgaac
     1261 tccagcaaac tacttctttg ccattccttt tttcttgccc ctgcccgcca tggcggactt
     1321 cttcggccct tttcgcgtcc gggcgttcgt tttcgttcgt tgacctcggg ccggcagacc
     1381 cttgcggtgc cgaagccccc ggtaactgcc gatgtccatc aaccgtttga tgtgcatcgt
     1441 cacatccctg cgcaggtctc cttccaccgt ataggagcgg tcgagaatag cccggatctt
     1501 gacgatgtct gactcggtca gattcccgct cttactgttc ggatcgacgc cggcctcggc
     1561 gagaattttc cgtgccgatg tcgggccgat gccatagatg tacgtgagcg caatctcgat
     1621 ttttttgtct ttggggagat ctaccccggc aatacgtgcc acatttcctc cttgagtatc
     1681 aactcatggc tagcagttcg gagcaactcc cgtcatccga aagctatgaa ctgcgaacca
     1741 tgagctacga gcttcatcac ccctgccgct gtttgtgccg ggcgttttca caaatcaccc
     1801 gcacaatgcc tttgcgtcga atgatggagc acttgggaca aatttttcgc actgatgaac
     1861 ggactttcat ggtctcttga ttccatttcc ttaacgtatt tatgcccaca attctgcggg
     1921 ccgcgcaatc cctatttaaa ccggtacgtg atcctgcccc tggtcagatc gtagggggtg
     1981 agttcgaccg ttactttatc tcccggaaga atcttgataa aatgcatcct catcttgccc
     2041 gatatgtgcg ccagaatctt gtggccgttc tccatctcga cgcgaaacat tgcgttagga
     2101 agtgtttcca gaaccgtacc ttgaacttct atcgaatctt cttttgccat atcctctcga
     2161 attccgcttc ccgcttcccg acgacttcag cctcccgcca cggcagctac ctcttcgtca
     2221 ggatccaggg accatccggc atcacggcga cggtatgttc aaaatgggcc gacaggctgc
     2281 cgtccgccgt aaccgccgtc cacccgtctt ccttcacgat cgtcgcgctc gcgcccctat
     2341 ttaccatggg ctccaccgcg agaaccatgc ccggctttag tcttggcccc tgcccctgcg
     2401 gcccgaaatt gggtatctgc gggtcctcgt gcaggctccg cccgattccg tggccgacaa
     2461 attcccgcac taccgagtaa ccgtgggcct cgacgtggtg ctggacggca tgtgagatat
     2521 cgtacagcct gtttccggcc ctcgcctgat cgataccccg gtagagcgac tcttccgtga
     2581 ccctgatcag gcgctcggcc gccggagcta tggtcccgac cggcagggta atcgcggcat
     2641 cgccgtaaaa cccttccacg attgccccaa cgtcaatgct gacgatgtca ccctgcttca
     2701 gcacgacgtt ctccgacggt atcccgtgaa tgacctcgta gttgaccgac gtacacagcg
     2761 tcttcgggta tccccggtag cctttgaacg ccggttttgc gcctgctgcg aggatccgtt
     2821 gctcggcgaa ttcgtcaagg gcttgcgtgc tgacccccgg ctggacaaga gcgcggatat
     2881 tgtccagtac gtctgcaacg acgagacagg cctgccggat tttttcaatc tcctgaaggg
     2941 acttgagaat gatcatgacc gctcccgggt ctagcgcctt cccttgatcg aaacgccctt
     3001 caggaaacct ccgtagtgcc tttgaattaa atgcgactct atctgagaaa ccgtatcgag
     3061 tgcaacacca attacgatca agagcgatgt cccgccgaaa tagaacggaa cattaaaaaa
     3121 cttgtaaaag atatccggca gcacacagac tgcggaaaga tacaacgccc cgccgagcgt
     3181 gatccggttc agcacccgga acaggtaatc ggaggtcttc tttcccggcc tgattccggg
     3241 gataaagccg ccgtacttct tcagattgtc cgcgatgtcc acagggttga acacgatcgc
     3301 cgtataaaaa taggcgaaga atatgatcat cagaacgtag a
//
LOCUS       DASSLD010000111        10399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_509992,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000111 DASSLD010000000
VERSION     DASSLD010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 10399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10399
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509992"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1956
                     /gene="msbA"
                     /locus_tag="VFK23_02085"
     CDS             217..1956
                     /gene="msbA"
                     /locus_tag="VFK23_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HET7317903.1"
                     /translation="MEIYKRLLVYLKPYRSRLLWSVLFMGITSGLISLQAYIVQPVLD
                     NVFLNKNMTLLILLPPALMIVVILKGASAYARDYLMGWIGQKIVNDIRDQLYSHLTSL
                     SFSFYTRTPTGVLISRIINDVNLVQGALTKAPSSLVQGIFTMMALTGYIIYKDWRLAA
                     FSVVVLPVLGIALSKFSKRFRHASTQMQEQYGDLTMHLHETISGIRIVKAFGMESYEN
                     GRFAERNKGLFNSLMRSIKTGAVSHPIMEVITTFGISTVILIGGYWIIRGNMTVGEFF
                     SFMTALYFLYRPIKDLNGVNSIVQDGVAAAKRIFEVLDTQAEIRDKPGAVDLSRDIKS
                     VEFRDLSFRYEDAPVLKGINLTVQAGEAIAIVGKSGGGKTTLVNLIPRFYDATGGSIL
                     IDGRDIRDVSIESLRSLTAIVTQQTFLFNDTVRANIAYGDRGRQFDDIIHAARSAYAD
                     DFIRALPQGYDTVIGESGVKLSGGQRQRIAIARALLKDAPILILDEATSSLDTESERE
                     VQKALERLMEGRTSFVIAHRLSTVMNADRIVVLKDGRIVEQGRHEELLSHGGEYKNLY
                     EQQFRDEPQVKTV"
     gene            2084..2746
                     /locus_tag="VFK23_02090"
     CDS             2084..2746
                     /locus_tag="VFK23_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7317904.1"
                     /translation="MSYIGWVVLYLWSRTVRIRKVNQEIPRALHDAGKNFIYAFWHGR
                     QFLLFHSHRNTGIVIPASESRDGEIQAQILKRFGFDVVRGSSKRKGERALLALVDGLR
                     KGKTIALAVDGPRGPLHEVKQGVTYLAGKLNKPIVPVVTGARRHWILGKIWDRYMLPK
                     PFTAGVVIYGNPIVVNGTANDELESKRRELEASLNSLMIKADGYFNTSEFGITLHPHK
                     HS"
     gene            2789..4051
                     /locus_tag="VFK23_02095"
     CDS             2789..4051
                     /locus_tag="VFK23_02095"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HET7317905.1"
                     /translation="MYVIYNIALAAATVLFFPVILFKLLTVPKYRGGLTQKLGRLRKG
                     VLKAVNGTRPIWIHAVSVGEVMAAHPLLRELRKKYPGRKLILSTVTVTGNYTARRRVP
                     EADAVFYFPFDYPCVIRRVIKGVNPEIVLVAETELWPNFFRELKRAGIPSAVINGRIS
                     PASHRNYLKFKPFFRQVFDNVTLFCMQSEGDAERIKDIGAGPEKVMITGNLKFDQKIP
                     TNAPNPVSVPAGRKIITAGSTHRGEEAALLDIFTQLRAKYPELTLIIAPRHPERFDEV
                     EGIINGAGYECQRRTKLKGPVRDVLLLDTIGELRTFYALCDIAFVGGSLVKVGGHNLL
                     EPAAMKKPVIFSRYMFNFKEISEALMSCGGGVMVKDKGELFAQFDRLLSEPESAKQMG
                     ERAFRVIEANSGSAKKTIDAIGRLLGAR"
     gene            4041..5114
                     /gene="lpxK"
                     /locus_tag="VFK23_02100"
     CDS             4041..5114
                     /gene="lpxK"
                     /locus_tag="VFK23_02100"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HET7317906.1"
                     /translation="MRAKSYLEDLMYGRKSSLFLGATLSVLSLLYRTGIGFRRALYKL
                     GVLRTRDLPCTVISIGNITLGGTGKTPMVIAVARLFAESQRRPAVVSRGYGRRNESET
                     LIISDGSTVLADAQSGGDEPVLIGTRLNGVPVIADRLRRRGAQVAVETFGCGIVILDD
                     GFQHIQLKRDLDIVLVDAAEPFGNGRLFPAGVLREPVGALKRAHAVVITRAETHGSLE
                     ELKKRIASVSDARIFTSVHRPVDLVHCRDSGRKPLSVLRGERVCAFSGIARPASFLDL
                     LKTLGAVIAADRSYPDHHAFSRSDLAEVYKMSADHRAGMIVTTEKDAVRLRELGPEGI
                     WALRIELVVNEQKEWEQFLLNTL"
     gene            5111..5695
                     /locus_tag="VFK23_02105"
     CDS             5111..5695
                     /locus_tag="VFK23_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7317907.1"
                     /translation="MNSAVFLDRDGTVIEEVGYLSDLKQLRLIPGAAAAIRGLNDAGF
                     KVVLVTNQSGVARGYFTEAFVRETHDLLEKMLGFEGARLDGVYYCPHHPKAGNSPYTA
                     ACDCRKPGTGMLEQAARELGIDIRASFVVGDKWSDVELGQRAGAHSVLVGTGYGPDDP
                     GNVRPAHLDDPDFTAGTITEAADWIIRQAAKDQR"
     gene            5692..6756
                     /gene="waaF"
                     /locus_tag="VFK23_02110"
     CDS             5692..6756
                     /gene="waaF"
                     /locus_tag="VFK23_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HET7317908.1"
                     /translation="MKLQAKKILVMRYRFIGDTVLTVPFLRNLRDAFPEARIDLMLEP
                     FSGQVIEGCPYVDRIIPFEIKTIHRYSSQSRRGKLAGYLQYWRQIRQERYDAAFVLKR
                     SLSSALLVWAAGVPRRIGFATEGREILLSDPVAYRHDQHEVRNFLDCLRTLDIPVSSE
                     ALELWPAPESDEKVAGLLSDAGWSPEDTKIIIHAAASLPAKQWPLERFAAVMKFLQQR
                     YHARFLYTGAKEDASLYREIEGQGPFHGLDLCGKTSLHENLSVYRACDLFFGVDSGPM
                     HMAAAVGVPVVALFGPTNELKWGPWGGGHTVLTKRLSCRPCKPHKCRNNACMREISVE
                     EAAEALDQKVATIMPKKARV"
     gene            6758..7534
                     /locus_tag="VFK23_02115"
     CDS             6758..7534
                     /locus_tag="VFK23_02115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7317909.1"
                     /translation="MTARLSIALITYNEEKNIRRTLESVKWADEIVVVDSGSTDGTAA
                     ICREYGAKVVHQDWLGFARQKNLAIDRTSGDWVLSLDADEPIEPELAQEIRSIITSPQ
                     PLDGYRIPRKTFFLGKQIRHGGWYPDYNLRLFRKGRGRFEERVVHEAISVQGRIGNVE
                     HAILHHAYPDLASYMDSINRYSSLSVDVMRKKGISRFKAGWINILFRPMLTFFHKYFL
                     RFGFLDGKHGLVLNLFHSYYVFAKYAKAWECRTADREQRA"
     gene            7546..8697
                     /locus_tag="VFK23_02120"
     CDS             7546..8697
                     /locus_tag="VFK23_02120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7317910.1"
                     /translation="MRVLFVNNFRRRGGGEEFLRDLLPALSAKGVKIGLVCRPATPLA
                     DMFKNSQVDVFPIERTGLSGLSAFIRTANVIRKNRYEIIAIQRVHDIIQSWLGSLLSG
                     RRPHLTYTVQVPEFVQSRILLRRVDEITTISRHIRDKIVSFDPGLASRTRIIHYGINL
                     DQFNTASVKRDMLRERFRLSPRTRIIGTVGDLWKNQIEFLDALAIIRREMPDIRFALV
                     ATEEDNEPAKAFKKRARELRLDDFLLWTGRLPKDEMREFYADIDLAINMHRNEGFGIW
                     VIEALAMGAPVISVNEGGVRDSLECCPAGILVHGGQDAVTASVLRILSDHDLRRKMSE
                     AGPLWVKERFNKQRMVDDYYQFYANLTSGTRYGAPGREQPECPHEQRNV"
     gene            8788..9894
                     /locus_tag="VFK23_02125"
     CDS             8788..9894
                     /locus_tag="VFK23_02125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7317911.1"
                     /translation="MKILFVNNVRYRCGGEEYLMDLLPGLSGKGATVGIVCRPNAPLA
                     TMFNGTSVHVYPAEKSGIRGIGSILKIARIIRQENYEIICIQRGHDIIQSLIASLLSG
                     RRTTLVLTVHIADFIKSRFLLGRMHRIVAISRYIRRKIEDFYPALSGRVSVINNGIDV
                     ITFNAANAKRGFIRGRYGISADTSLISTCGIMWKNQIEFLDALAEIKREIPRVRYLLL
                     TPLGDSPQVREFNDRAAELGLTDSILWLNSLPKGEMPSYYADLDLAVSTFRNEGFGLW
                     VVEALAMGIPVVAFDGGGVRDILEDSPAAVLIKDGAGEMAREVIRILRNGETLKRMAD
                     AAPKWVAGRFSRERMVEDYFNLFRGLSTSSSTLN"
     gene            10037..>10399
                     /locus_tag="VFK23_02130"
     CDS             10037..>10399
                     /locus_tag="VFK23_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7317912.1"
                     /translation="MYHNIGKPPKGVETPNLYVTPGMLKFQMWYLKQAGFHVVTVQDL
                     MRFIGEGRTGGRMVALTFDDGYRDFYENAYPVLKYYGYPSTVFVTPGLMGGENVWDCI
                     REKVRKPLMDGDTVAEISR"
BASE COUNT         2320 a         2918 c         3044 g         2117 t
ORIGIN      
        1 acaaaattgc taaacgactc cgcatattac ggacgggtgc aggcccggct cgccgacata
       61 cgagcaaagc tcggcgaatc ggggacgaac gcgcgggtcg catcgctcgt ccgggagttc
      121 ctgggagaag aaggaccgaa gcggtgaaag ggaccggttt tttgcgtcgg ctccgcgatt
      181 cgccgtttta tcgattcgat taacgtgttt aaaactatgg aaatctacaa aaggctcctc
      241 gtatacctga agccctaccg gtcgcggctc ctttggtccg tcctcttcat ggggatcacg
      301 agcggcctga tctccctcca ggcctacatc gtgcagccgg tcctggacaa cgtcttcctg
      361 aacaagaaca tgacgctcct catcctgttg ccgccggccc tcatgatcgt ggtcattctc
      421 aagggggcat cggcgtacgc gcgggactat ctgatgggct ggatcgggca gaaaatcgtg
      481 aacgatatcc gcgatcagct ctattcgcac ctgacgtctc tttccttttc cttttacacc
      541 aggacgccca ccggcgtcct catctcccgg atcatcaacg acgtgaacct tgtccagggg
      601 gcgctgacca aagcgccctc gagcctcgtg caggggatct tcaccatgat ggccctcacg
      661 ggatacatca tctacaaaga ctggcggctt gccgcattct ccgtggtggt cctgccggtc
      721 ctcggcatcg ccctctcgaa attcagcaaa cggttccgtc atgccagcac gcagatgcag
      781 gagcagtacg gcgacctgac catgcacctt cacgagacca tctcggggat ccgcatcgtc
      841 aaggccttcg gcatggagtc ctatgagaac gggaggttcg ccgaacgcaa caaggggctc
      901 ttcaattcgc tcatgcgctc catcaagacg ggggcggtct cgcacccgat catggaggtc
      961 atcaccacgt tcgggatctc gacggtcatt ctgatcggcg gctactggat catccgcggc
     1021 aatatgacgg tcggtgaatt tttctcgttc atgacggccc tgtattttct gtaccggccg
     1081 atcaaggatc tgaacggcgt gaacagcatc gtgcaggacg gcgtggcggc ggcaaagcgc
     1141 atcttcgagg tcctcgacac gcaggcggag atcagggaca agccgggcgc ggtcgacctg
     1201 tcccgtgaca tcaagagcgt ggagtttcgg gacctttcct tccggtacga ggacgcgccc
     1261 gtcctcaagg gcatcaacct cacggtgcag gcgggcgagg cgatcgcgat cgtcgggaaa
     1321 agcggcggcg gcaagacgac gcttgtcaac ctcatcccgc gcttctacga cgccaccggc
     1381 ggatcgatcc tcatcgacgg cagggacatc agggacgttt ccatcgagtc gctccgctcc
     1441 ctgacggcca tcgtcaccca gcagaccttt ctgttcaacg acaccgtgcg ggcgaacatt
     1501 gcttacggag accggggcag gcaattcgac gatatcatcc atgccgcccg gtcggcctat
     1561 gcagacgatt tcatcagggc cctcccgcag gggtacgaca ccgtcatcgg agagtccggc
     1621 gtaaagcttt ccggaggaca gcggcagcgg atcgccatcg cccgggcgct ccttaaagac
     1681 gctcccattc tgatcctgga cgaagcgact tcgtcgctcg ataccgagtc ggaacgcgag
     1741 gtgcagaagg ccctcgaacg gctcatggag ggcaggacga gtttcgtcat cgcccaccgc
     1801 ctgtcaaccg taatgaacgc agaccggatc gtcgtgctca aagacggaag gatcgtcgag
     1861 cagggcagac atgaggaact gctttcgcac ggaggggaat acaaaaatct ttacgaacag
     1921 cagtttcgcg acgaacccca ggtgaaaacg gtatgattct ggatactgga ttgattcttg
     1981 ataccggata ccggatagcg gacagggtgt tatccagcat ccggtatcag gcatccagca
     2041 tcggcttttt gagagaaact gcataatgct gagagcacgg atattgtctt acataggctg
     2101 ggtagtcctg tacctgtgga gcaggaccgt caggatcagg aaagtgaacc aggaaatccc
     2161 gcgggccctc catgacgcgg ggaagaattt catctatgcc ttctggcacg gaaggcagtt
     2221 tttgctgttc cacagccacc ggaacactgg catcgtgatc ccggcaagcg aaagccgcga
     2281 cggcgagatc caggctcaga tccttaagcg gttcgggttc gacgtggtgc gcggctccag
     2341 caaacggaag ggcgaacggg cgctcctcgc gctggtcgac ggcctccgaa aggggaaaac
     2401 gatcgcgctc gcggtggacg gtccgcgcgg ccccctccac gaggtgaagc agggtgtgac
     2461 ctatctggcc ggaaaactga acaagccgat cgttcccgtg gtgaccggcg caaggcggca
     2521 ctggatcctg ggaaagatat gggaccgcta tatgcttccc aagcccttta cggcaggtgt
     2581 ggtcatctac ggtaacccga tcgtcgtgaa cggaacagcg aatgacgaac tcgagtccaa
     2641 gcgccgggag ctggaagcgt ccttgaacag cctgatgatc aaggcggacg gatattttaa
     2701 cacttcggaa ttcggcatca ccctgcaccc gcacaaacac tcgtaaaacc gtgtaaagtt
     2761 cggaatgcgg aataattgac ggaacgacat gtatgttatt tataacatag cgctggccgc
     2821 cgcgacggtc ctcttctttc cggtgatcct gttcaaactc ttgaccgttc ccaagtaccg
     2881 gggggggctg acccagaaac tcggccgtct gcgcaaaggc gtcttgaagg cggtcaacgg
     2941 gacgcggccg atctggatcc acgcggtatc ggtcggcgag gtcatggccg cccatccgct
     3001 gctccgggag ttgaggaaaa aatatccggg gcgcaagttg atcctctcta ccgtgaccgt
     3061 gaccgggaat tatacggccc gccgaagagt tcccgaggcg gacgcggtct tttattttcc
     3121 gtttgactac ccttgtgtca tccgcagggt gatcaagggc gtcaaccccg agatcgtgct
     3181 cgtcgccgaa accgaactct ggccgaactt cttccgggaa ctgaagcgcg ccggcatccc
     3241 ttcggccgtg atcaacggga ggatatcgcc ggcctcccac aggaattatc tcaaattcaa
     3301 accgttcttc cgccaggtct tcgacaacgt gacgctgttc tgcatgcagt ccgagggaga
     3361 cgcggaaagg atcaaggaca tcggcgccgg tccggaaaag gtgatgatca cggggaacct
     3421 caagttcgac cagaagatac ccacgaacgc gcccaacccg gtctccgtgc cggcaggaag
     3481 aaaaatcatc accgccggaa gcacccaccg cggagaagag gccgccctgc tcgacatatt
     3541 tacccaactt cgggcaaaat atcccgaact gacgctgatc atcgccccac ggcaccctga
     3601 acgcttcgac gaggtggagg gcatcatcaa cggagcaggc tatgaatgcc agcgcaggac
     3661 gaagctcaaa ggtcctgtca gggacgtgtt gcttctcgac acgatcggcg agctcaggac
     3721 tttttatgca ctttgcgaca tagccttcgt gggcgggagt ctcgtcaagg tcggaggaca
     3781 taatcttctt gagccggctg cgatgaaaaa gccagtgata ttcagccgtt acatgtttaa
     3841 ctttaaagaa atatcagagg ccctcatgtc atgtggagga ggggtcatgg taaaagacaa
     3901 gggtgagctg ttcgcccagt tcgacagact cctttcggag cctgaaagcg caaagcaaat
     3961 gggcgagcgt gcgttccggg tgatcgaggc caactcaggc tccgcgaaga agacgatcga
     4021 tgcaatcggc aggctcctcg gtgcgcgcta agtcatacct tgaagacctg atgtacgggc
     4081 gaaaaagctc ccttttcctg ggggcaacat tgtccgtgtt gtccctgctg taccggaccg
     4141 gcatagggtt ccgtcgtgcg ttgtacaaac tgggtgtcct cagaacccgc gacctgccgt
     4201 gcacggtcat ttccatcggc aacataaccc tcggcgggac aggcaagacc cctatggtga
     4261 ttgccgtggc ccgtctcttc gcggaatcgc aaagaaggcc cgcagtggtg agccgaggct
     4321 acggtcgaag aaacgaatcg gagaccttga tcatctccga cgggagcacg gtcctcgcgg
     4381 acgcacagag cgggggggat gaaccggttt tgatcggcac ccgtctgaac ggcgtgcctg
     4441 tcatcgctga caggcttcgg cggcgcggcg cacaggttgc cgtggaaacg ttcggctgcg
     4501 gaatcgtcat ccttgacgac ggttttcagc acattcagct caagcgggac ctggacatcg
     4561 tgcttgtgga cgcagcggaa cctttcggga acggcagact ctttccggca ggcgttttgc
     4621 gcgagccggt gggcgctctc aagcgggccc acgcggtggt cattacgagg gcagaaacgc
     4681 acggttccct ggaggagttg aagaagcgca tcgcgtccgt atcggacgcg cgtatcttca
     4741 cctcggtcca ccggcctgtc gatctcgtcc actgccggga ctccgggcgc aaaccacttt
     4801 ccgtcctgcg cggtgaacgg gtgtgcgctt tttcgggcat tgcccggcct gcgtcgttcc
     4861 ttgacctgct gaagacgctt ggggccgtca tagcagcgga caggtcgtat ccggaccacc
     4921 atgctttttc acgttccgac ctggcggagg tctacaaaat gtccgctgat caccgggcgg
     4981 gcatgattgt caccaccgaa aaggacgctg tccgtctgcg ggagctgggg cccgagggga
     5041 tatgggcgct gcggatcgaa cttgtcgtca atgaacagaa ggaatgggaa cagtttctct
     5101 taaacactct atgaacagtg cggtatttct cgaccgagac ggcaccgtca tcgaagaggt
     5161 gggatacctc agcgacctga agcagctcag actcatcccc ggggccgccg ctgcaatcag
     5221 aggcctcaat gatgcggggt tcaaggtcgt cctcgtcacg aaccagtcgg gcgttgcgcg
     5281 ggggtatttt accgaggcct ttgtgcgtga aacgcacgac ctcctcgaaa aaatgctcgg
     5341 tttcgaggga gcacgtcttg acggcgtcta ttactgtccg caccatccga aggcggggaa
     5401 ctcgccctat acggccgcat gcgactgcag aaagcccggc accggcatgc tcgaacaggc
     5461 ggcccgggag ctgggtatcg atatccgggc gtcattcgtg gtcggggaca aatggagcga
     5521 tgtggaactc gggcagcgcg cgggagcgca ctccgtcctg gtcgggacgg ggtatgggcc
     5581 cgacgatccc ggaaatgtgc ggcctgcgca tctggacgat ccggacttca ccgccgggac
     5641 catcaccgaa gccgctgact ggatcatccg gcaggcagcg aaggatcaac ggtgaaactg
     5701 caagcaaaaa aaatacttgt gatgcggtac cgcttcatcg gcgatacggt gctgactgtg
     5761 ccctttctgc gtaacctgcg cgatgccttt cccgaggccc ggatcgacct catgctggaa
     5821 ccgttttccg ggcaggtgat cgaaggctgt ccgtacgtgg atcgcatcat ccctttcgag
     5881 ataaaaacaa tccaccggta cagcagccaa tcgcggcgcg gcaagctggc gggatatctt
     5941 cagtattgga ggcagatacg gcaggagcga tatgacgctg cattcgtgct gaagagatcg
     6001 ctttcgagtg cgctgctggt ctgggcggct ggagttcctc ggagaatagg ctttgccacc
     6061 gaaggaaggg aaatcctgct ttccgatccc gtggcctacc ggcacgacca gcatgaggtc
     6121 cggaacttcc tcgactgcct gcgcacgctc gatattcccg tctcttcgga ggcgcttgaa
     6181 ctctggcccg cgcccgaaag cgacgagaag gtggccggcc tccttagcga cgcgggttgg
     6241 agtccggagg acacgaagat catcatccac gcggcggcaa gtcttcccgc gaagcaatgg
     6301 cccctcgagc ggtttgcagc ggtcatgaaa tttctccagc agcgctatca cgcccgcttt
     6361 ctctacacag gtgctaagga agacgcgtca ctctaccggg aaatcgaagg ccaggggccc
     6421 tttcatggct tggatctctg cgggaagacc tctctccatg agaacctctc ggtgtatcgc
     6481 gcctgcgacc tgttctttgg agtcgattcg ggacccatgc atatggcggc ggcggtcggc
     6541 gttccagtag ttgccttatt cggtccgacc aacgaactca agtgggggcc ctggggcggg
     6601 ggccacaccg tcctcaccaa gaggctatcc tgccgcccct gtaaacctca taaatgcagg
     6661 aacaatgcgt gcatgcgtga gataagcgtt gaagaggcag ccgaggcctt ggaccaaaag
     6721 gtagcaacga taatgccgaa gaaagcgcga gtctgacatg acggcaaggc tatccatagc
     6781 cctcatcacc tataacgaag agaagaacat ccgacggaca ctcgagagcg tgaaatgggc
     6841 tgacgaaatc gttgtcgtgg attcggggag cacggacggc accgccgcga tctgccgtga
     6901 atatggggcc aaggtcgttc atcaggactg gctcggtttc gcacgccaga agaaccttgc
     6961 catagaccgc acctccggtg actgggtttt aagccttgat gccgacgagc cgatcgaacc
     7021 ggaactggcg caggaaatca ggagtatcat tacatccccg cagcctctgg atggataccg
     7081 tattccccgc aaaacctttt tccttgggaa gcagattcgc cacggcggct ggtatccgga
     7141 ttacaatctc cggctcttcc gcaaagggag gggacggttc gaggaacggg ttgttcatga
     7201 ggcgatcagt gttcagggac gaataggaaa cgtagagcat gccatcctgc atcacgccta
     7261 ccctgacctt gcttcctaca tggactcgat caaccgatat tcatcgctct ccgttgacgt
     7321 gatgagaaaa aaaggaatca gccgtttcaa ggcagggtgg atcaacatcc tgttccgccc
     7381 catgctgacc tttttccaca agtattttct tcggttcggt ttcctcgacg gaaagcacgg
     7441 gcttgtcctg aacctgtttc attcttacta cgttttcgcc aagtacgcca aggcgtggga
     7501 atgcaggact gcggatcggg aacagagggc ataggatagc atacgatgag ggtcctgttt
     7561 gttaataatt tcagaagacg gggcggaggc gaagagtttc tccgcgatct cctccccgct
     7621 ctttccgcaa aaggggtgaa gatcggactc gtatgccgtc ccgcgacgcc ccttgccgac
     7681 atgttcaaga acagccaagt cgacgtcttc ccgatcgaac ggaccggcct gagcggttta
     7741 agcgcattca tacggacggc gaatgtaatc agaaagaacc gatatgagat catcgccatc
     7801 cagcgtgtgc acgacatcat ccagtcctgg ctcggcagcc tgctttcagg caggagacct
     7861 catctcacct ataccgtcca ggtaccggaa tttgtccagt cacgtattct cctgcgccgc
     7921 gtagatgaaa ttactacgat ctcccggcat atcagggaca aaatcgtgtc cttcgatccg
     7981 gggcttgctt cgaggacccg catcattcat tacggcatta acctcgatca gttcaatact
     8041 gcatcggtaa agcgggacat gctgagggag cgcttcaggc tttcgccccg gactaggatt
     8101 atcgggacag taggagacct ctggaagaac cagatcgagt tcctcgacgc cctggcaatc
     8161 atccggagag agatgcccga tatccggttt gcgctcgttg caaccgagga ggacaacgaa
     8221 cccgccaaag cttttaaaaa acgggcccgt gaactgaggc ttgatgattt cctgctctgg
     8281 accgggaggt tgccgaagga tgagatgagg gaattttacg cggacatcga tcttgcgatc
     8341 aacatgcatc gcaacgaagg cttcggtatc tgggtgatcg aagccctggc catgggtgcg
     8401 ccggtcattt ccgtgaacga ggggggggtc cgtgattctc tcgaatgctg tcctgccggc
     8461 atcctcgtac acggcggcca ggacgccgtg actgcaagcg ttctccggat cctttctgat
     8521 catgatctga ggagaaaaat gtcggaagcg ggtccgctct gggttaagga acgcttcaac
     8581 aaacagagga tggtggatga ttattaccaa ttctatgcga acttgacatc gggtacccgt
     8641 tatggagcgc caggcagaga acagcctgaa tgtcctcatg aacagagaaa cgtatgacgc
     8701 gattatccat ggcttcgatt ccttgctttt aacaaggcat atatgtaaga tgtctcttga
     8761 tatttcaagg cagttccccg ggatgaaatg aaaattctct ttgtgaacaa tgtcagatac
     8821 cggtgcggcg gcgaggagta cctcatggac cttctccccg gtctgagcgg aaaaggcgca
     8881 acagtgggca tcgtgtgccg ccccaatgcg ccgctcgcaa ccatgttcaa cggcacgtct
     8941 gttcatgtct atcccgccga gaaatcggga atccgcggga tcggatccat tctcaagatc
     9001 gcgcggatca tcaggcagga aaactacgag atcatctgca ttcagcgcgg gcacgacatc
     9061 atccagtcct tgatcgcgtc cctcttgtcc ggcagaagaa cgactctcgt cttgactgtg
     9121 cacatcgctg attttatcaa gtcgcgcttt ctcctgggcc gcatgcacag gatcgtcgcg
     9181 atctcccggt acatccgccg gaagatagaa gacttctatc ccgcgttgtc gggcagggtc
     9241 tccgtcatca acaacggcat cgatgtcatc acattcaatg ccgcaaatgc gaaaaggggg
     9301 tttatcaggg gcagatacgg catctcagcg gacaccagcc tgatcagcac gtgcggcatc
     9361 atgtggaaaa atcaaatcga atttctggac gccctcgcag agataaaaag ggagataccg
     9421 cgcgttcggt atctgctgct cacccccctg ggagactcgc cccaggtgcg ggaattcaac
     9481 gaccgcgccg ccgaactggg cctgaccgat tccatcctgt ggctgaacag cctgccgaag
     9541 ggagaaatgc cctcttacta tgccgatctc gatctcgccg taagcacctt tcgcaacgaa
     9601 ggcttcggcc tgtgggtcgt ggaagccctg gcaatgggga tacccgttgt cgcctttgac
     9661 ggcggcggcg tgcgggacat tctcgaagac tcacccgcgg ccgttttgat taaagatggg
     9721 gcgggggaaa tggccaggga ggttatacgt attttaagaa acggggaaac gctcaaaaga
     9781 atggccgatg cggcaccgaa gtgggtcgcc gggcgattca gccgcgaaag aatggtggag
     9841 gattatttca atttatttcg ggggctttcc accagttcgt cgacgctcaa ttgatatatc
     9901 gaggagagaa gcgtttccct gctcttgcga aacgtcggga caatagggag tgacgacaca
     9961 ggagtaaacg ttaagccgct gcagctattc cgattcaaaa ggggacagag ccgaagtgtc
    10021 acacgcaatt attctcatgt atcacaacat cgggaaaccc ccaaaggggg tggaaacgcc
    10081 caatttatac gtaacgccgg gaatgcttaa attccagatg tggtacctga aacaggcggg
    10141 cttccatgtt gtcaccgtgc aggaccttat gcgtttcata ggggaaggcc gaacaggcgg
    10201 caggatggtc gccctcacat tcgacgacgg ttatagggac ttctatgaaa acgcatatcc
    10261 tgtcctgaaa tactacggtt atccttccac ggtttttgtc acgccggggc tcatgggcgg
    10321 ggaaaatgtg tgggattgca tacgagaaaa agtcaggaaa ccgctgatgg atggggacac
    10381 cgtcgccgaa ataagcaga
//
LOCUS       DASSLD010000112         5966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1228055, whole genome shotgun sequence.
ACCESSION   DASSLD010000112 DASSLD010000000
VERSION     DASSLD010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228055"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VFK23_02135"
     CDS             <1..696
                     /locus_tag="VFK23_02135"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET7317913.1"
                     /translation="AIAELRKYEKNTSEYRTVHAFLSAVEREATEVLLQSLAEEEDRA
                     RRIFILDLAKDLGKNHIALLGGHLSDERWYVVRNIVNILSETKTDQAIAFLRRAAEHK
                     NIRIRQEVIKGLLSIGGKKAAGVMAKFLRDNDADIMATAVRAFADLPGIGAEETKPLL
                     AFLEDRPLKKKDLEVSLETIGVLGKIGGKDAAEFLKRYDRIRWWKPRTLQRELRAAAA
                     RARAHIERRTADG"
     gene            689..2122
                     /locus_tag="VFK23_02140"
     CDS             689..2122
                     /locus_tag="VFK23_02140"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HET7317914.1"
                     /translation="MAEENSDSRGEARQSFYAKQRLINSARSAFQQLAAIMKNATLYP
                     ETHPFLMASADKLRSSVDELLVGRKEVAFYLVHGELFFEILSDPIDESLTQLMEQFTN
                     RGIGGIVFKPGLTSKELVGFARLLNNDQSFSTERGGVIDAIAREGISHIDLHQVLLVD
                     KKAGDAIKEGRKKATEVFQDAIAAVKEMVEAVHLDKTARMRKMNTMVQTMVDNILDNR
                     DALMGLTSIKMYDEYTFAHSVNTAILAVSLGTYLSFEKNQIAALGVAGLMHDIGKVNV
                     PHEVINKPGKLTEEEWQLVKQHPVEGALMLSDIPGVSKLAMVAAFEHHQHNDAFGYPK
                     VDGEMRQHPFSQIISLADAYEALTAARVYYSVQMPPDEAIRIILAKRGSNFNAAIVKA
                     FVNMIGIFPIGTLLKLDTGEAGLVIHQTRDLMRPRVLLLDKFDGSEKETGEETSLLET
                     SGGKYKRTATGIINPYAAGINIKQYLE"
     gene            2200..3702
                     /locus_tag="VFK23_02145"
     CDS             2200..3702
                     /locus_tag="VFK23_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HET7317915.1"
                     /translation="MKILLVYPECPETFWSFKYALRFISKHASLPPLGLLTVAAMLPA
                     GWDLRLADMNVRKLTDRDLRWADFVFLSAMSIQKVSVLSVIARCKALGVKVIAGGPLF
                     TAMPDEFIDVDHLILNEAEVTLPLFLEDLQKGRLRRVYSSTQWADLSKTPVPRFDLLN
                     KKRYASMNVQYSRGCPFDCEFCNITSLFGRTPRTKTGGQLLAEFERLYQGGWRHGVFI
                     VDDNFIGNKGKLKKEVLPAIISWMEERGHPFELSTEASINLADDEELMQLMVRAGFDT
                     VFVGIETPNEESLAECSKIQNRNRDLIESVKKIQRAGLEVQAGFIVGFDKDPTTIFER
                     LISFIQESGIATAMVGLLNAPKGTKLYNRLMSEGRLVKAATGDNTDFSINFVPKMPSD
                     VLLNGYRRIVHTIYSPKYYYARVREFLREYRPVKQKKIRLRWSYTKAGFKSVVRLGIV
                     GRERYQYWKLFLWSLLFRPRLLGMAITYSIYGFHFRKVFKRQEKFRGMGA"
     gene            3844..>5966
                     /gene="hrpB"
                     /locus_tag="VFK23_02150"
     CDS             3844..>5966
                     /gene="hrpB"
                     /locus_tag="VFK23_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HET7317916.1"
                     /translation="MKTSYPIDSVIPRLITTIRQNPSVLIIAPPGAGKTTRVPLALLD
                     VIPPEEGRIVMLEPRRIAAVSAARWMAKSIGEEAGDTVGYAIRFDTKRSAKTRIEVVT
                     EGILTRRLQADPALEGTAMVIFDEFHERSIHADLALALCLDVRKALRPELKILVMSAT
                     LDYGPLQALLEGAPVVLSAGKSFPVEDRWLADEQGPLPVRIARAVRTALRETLGDILV
                     FLPGAGEIRACAKELNGTLDLSGDRIALHTLYGDLPFDEQERAIMPSPDRRKIVLATN
                     IAETSLTIEGVQVVIDSGLTRILRYDPATGMNRLATVPVSRASAEQRKGRAGRLGPGV
                     CFRLYGSHELSGLPPFTQPEIIISDLSQLALELSLWGVKDPAALSWLDAPPRAAWDSG
                     MRLLAELGAIDGTGSITHAGRSMARLPVHPRLGRLMILAQELGCPRLGTDLAALLTER
                     DVFRHSSAVRTPEEPDVMERIEALRSWRQNKESGKADPSALRAVERTSKQLLRLLPDD
                     LREDDGVDPHAVSRLLLSAFPDRICKRRDEGGGRFVHMQGRGVRLSAESHLGGSPYIV
                     AVAADAGEKTEGYVHIAVPVTEDLIRRECGQRIETVRRVEWDWKENRIAAAVEERLGA
                     LLFSKRPFVPTDEEAAPILLDVVRTTPSVLTFSKEARQFQARVGLLGRVFPAEAWPDL
                     SDEQLLTKPQEWLLPWLGGVRTGQAL"
BASE COUNT         1247 a         1781 c         1824 g         1114 t
ORIGIN      
        1 gcaatcgcag agctccggaa gtacgaaaag aacacttccg agtaccggac ggtgcatgcc
       61 ttcctgtccg ccgtcgagcg ggaggcgacg gaggttctgc tgcagtccct ggccgaggag
      121 gaagaccggg ctaggcgcat attcatcctc gacctcgcga aggatctcgg caaaaaccat
      181 atcgcgctgc tcggcgggca tctgtcggac gaacggtggt atgtcgtgcg caatatcgtc
      241 aacatcctga gcgagaccaa gacggaccag gccattgcgt ttctccgaag ggcggcggaa
      301 cacaagaaca tccgcatcag gcaggaagtg atcaaggggc tcctgtccat aggcgggaaa
      361 aaggccgccg gagtgatggc gaagttcctg cgggacaatg acgccgacat catggcgacc
      421 gccgttcgcg ctttcgccga cctgcccggc atcggcgcgg aggagaccaa accgctgctt
      481 gcgttcctcg aggaccggcc gctcaagaag aaggacctgg aggtctcgct cgagacgatc
      541 ggggtcctgg ggaagatcgg cggtaaggac gcggcggaat ttctcaaacg atatgaccgc
      601 atccggtggt ggaagccccg gactcttcag cgtgagctgc gtgccgcagc ggcccgggcg
      661 cgggcgcaca tcgaaaggag aacggccgat ggctgaagag aacagcgata gccggggaga
      721 ggcgcgtcag tctttctacg cgaaacagcg tctgatcaac tcggccaggt cggccttcca
      781 gcagctggcg gccatcatga aaaacgcgac cctctatccc gagactcatc ccttcctcat
      841 ggcttccgcg gacaagctgc ggagcagcgt agacgagctg ctggtgggcc ggaaggaggt
      901 tgccttctac ctggtgcatg gtgagctgtt tttcgaaatc ctgtctgatc cgatcgacga
      961 gagcctcacg cagctcatgg aacagtttac gaacaggggc ataggcggta tcgtcttcaa
     1021 gccgggtctg acgtccaagg aactggtcgg ttttgcccgc ctgctgaaca acgaccagtc
     1081 gttctccacc gagcggggag gagtcatcga tgcgatagcc cgggagggca tctcacatat
     1141 agatcttcac caggtgcttc tcgtcgacaa gaaggcgggg gacgcaatca aggagggaag
     1201 gaaaaaggcg accgaggtct tccaagacgc cattgcggcc gtaaaagaaa tggtcgaggc
     1261 cgtgcatctc gacaaaacgg ccagaatgcg caaaatgaac accatggttc agaccatggt
     1321 cgacaatatc ctggacaatc gcgacgcgct catggggctt accagcatca agatgtacga
     1381 tgagtacacc tttgcccact cggtgaacac cgcgatcctg gccgtctccc tcggcaccta
     1441 cctgtccttc gagaagaacc agatcgcggc gctcggcgtc gccggtctca tgcatgacat
     1501 cggcaaggtg aacgtccccc acgaggtcat caacaagccg ggcaagctca cggaagagga
     1561 atggcaactg gtcaagcagc atcccgtcga gggggccctg atgctctccg acatccccgg
     1621 cgtttccaaa ctggccatgg tcgcggcctt tgaacatcat cagcacaacg atgcgttcgg
     1681 ttatccgaag gtcgacggcg aaatgaggca acaccccttc tcgcagatca tctccctcgc
     1741 ggacgcctat gaggcgctca cggccgcccg cgtgtattac agcgtgcaga tgccgcctga
     1801 cgaggcaatc cgtatcattc tcgcgaagcg cggctctaac ttcaatgccg ccatcgtcaa
     1861 ggcattcgtc aacatgatcg gaattttccc catcggcaca ctcctcaaac tggacacggg
     1921 cgaggccggc ctggtcatac atcagacgcg cgacctgatg cggccccggg tcctgctgct
     1981 cgacaagttc gacggctccg agaaagaaac cggagaggag accagtttgc tcgagacctc
     2041 gggagggaaa tacaaacgca cagccaccgg catcattaat ccttatgccg ccggcatcaa
     2101 cataaagcag tacctggaat aactgcagcg ggattccgtc ccatgcgggc ggactcagtt
     2161 ttccggtcgt cacatcgctg caagaggaga agaactgtca tgaaaatact gctcgtctat
     2221 cctgagtgtc ccgaaacgtt ctggagtttc aagtatgcgc tgcggttcat ctccaagcat
     2281 gcaagccttc ctccgctcgg gctcctgacg gtagcagcca tgcttccggc tgggtgggac
     2341 ctccggctcg ctgacatgaa cgttcggaag ctaacggaca gagacctccg gtgggctgac
     2401 tttgtttttt tgagcgccat gtccatccag aaagtgtcgg tcttgtctgt gatcgcacgg
     2461 tgcaaggcct tgggtgtaaa agtgatcgcc ggcggtcccc tgtttacggc gatgccggat
     2521 gagttcatcg acgtagacca tcttatcttg aacgaggcgg aggtaacgtt gccgctcttt
     2581 ctggaagacc tgcaaaaggg aaggctgcgc cgcgtctaca gctccacgca gtgggcagac
     2641 ctttcgaaga cgccggtacc tcggttcgat ctcctgaata aaaagaggta cgcctccatg
     2701 aatgtccagt attcgcgggg ctgccccttc gactgcgagt tctgcaacat cacatccctg
     2761 ttcggccgca ccccgcggac gaagaccggc gggcagctcc tcgcggaatt cgagcgcctc
     2821 taccaggggg gatggaggca cggcgtattc atcgtcgacg acaacttcat cgggaacaag
     2881 gggaagctca agaaggaggt gctgccggcc atcatctcct ggatggagga gcggggccat
     2941 cccttcgagc tctcgacgga ggcgtccatc aacctggcgg atgacgagga actgatgcag
     3001 ctcatggtgc gcgccggttt cgacacggtc ttcgtgggca tcgagacccc gaacgaggag
     3061 agcctggcag agtgcagcaa gatccagaac aggaacaggg acctcatcga gagcgtgaag
     3121 aagattcagc gcgccgggct tgaggtccag gcgggcttca tcgtgggatt cgacaaggac
     3181 ccgacaacga tcttcgaacg gctcatctcg tttattcagg aaagcgggat cgcaaccgcc
     3241 atggtagggc tcctgaacgc gccgaagggc acgaaactgt acaaccgcct catgagcgaa
     3301 ggccggctcg taaaggccgc aaccggcgac aacactgatt tctcgatcaa cttcgtgccc
     3361 aagatgcctt ctgacgtgct gttgaacggc taccgaagga tcgtccacac gatctattcc
     3421 ccgaaatact actacgcacg ggtgcgggag ttcctgaggg aataccggcc cgtaaagcag
     3481 aaaaagatcc gcctgcgctg gagctacacg aaggcgggat tcaagtccgt cgtgcgcctc
     3541 ggcatcgtcg gcagggagcg gtatcagtac tggaagctct tcttatggtc gcttctgttc
     3601 aggccgcgtc tcttggggat ggccataacg tactcgatat acggatttca tttccgcaag
     3661 gttttcaagc gccaagaaaa gttcaggggc atgggggcgt aggggctttt gggaaaagac
     3721 tgcaggggga agaggggagg gccagtaggt gggagaagac catggccact gccgagaggc
     3781 tcttccagtc ccctcttccc agcctctcac cttccacgct cgagattccc gcgttaaatc
     3841 cccatgaaaa catcctatcc catcgacagc gttattccaa gactcataac aacgattcgg
     3901 cagaacccct ccgtccttat catcgcgcct cccggagccg ggaaaacgac gagggtcccg
     3961 ctcgcgctac tcgacgtcat tccgcccgaa gaaggccgca tcgtcatgct cgaaccgcga
     4021 aggattgcgg ccgtttccgc cgcgcggtgg atggcgaaat cgatcggcga agaggcgggt
     4081 gataccgtcg gctatgctat tcgcttcgac acgaaaagat cggcgaagac acgcatcgag
     4141 gtcgtcactg aaggcatcct tacccgcagg ctccaggccg accccgctct cgagggcacg
     4201 gccatggtca ttttcgacga gttccatgaa cggagcattc atgcggacct ggccctcgcg
     4261 ctttgtctcg atgtccgcaa ggcgctccgg cccgaactca agattctcgt catgtccgcc
     4321 accctcgact acggaccgct ccaggcgctt ctggaaggcg cgccggtggt cttatccgca
     4381 ggaaagtcct ttccggtgga agatcgctgg ctcgcggacg agcaggggcc tctgcccgtg
     4441 cggattgcgc gcgcggtcag aacggcgctc agggagaccc tgggcgatat cctcgtgttc
     4501 ctgccggggg ccggcgagat cagggcctgt gcgaaggaac tgaacgggac gctggacctc
     4561 tccggcgacc gcatcgccct tcacaccctt tacggagacc tgccgttcga cgagcaggag
     4621 cgcgccatca tgccgtcgcc ggacaggcgc aagatcgttc tcgccacgaa catcgccgag
     4681 acgagcctta ccatcgaggg cgtgcaggtc gtcatcgaca gcggtctcac gcgcatcctc
     4741 cgctatgacc ccgcaacggg catgaatcgg ctggccaccg tccccgtctc gcgggcatcg
     4801 gcggaacagc gcaagggcag ggcgggaagg ctcggacccg gcgtctgctt tcgcctgtac
     4861 ggcagtcatg agctttccgg attgccgccg ttcacccagc cggagatcat catctccgat
     4921 ctttcccagt tggcgctcga actctccctc tggggtgtga aggacccagc cgccctttca
     4981 tggctcgacg cgccgccaag ggcggcgtgg gattcaggta tgcggcttct cgccgaactg
     5041 ggcgctatcg acgggacggg ttctataacc catgctggca ggtctatggc ccgcctcccg
     5101 gtccatcccc gtctgggccg cctcatgata ctggctcagg aactgggctg tccccgcctc
     5161 ggcacggacc tcgccgcgct tttgaccgaa cgggatgtct tccgccacag ctccgccgtt
     5221 cgcacgcctg aagagcctga cgtcatggag cggatcgaag ccctccgctc gtggcggcag
     5281 aacaaagagt ctggaaaagc agacccgtct gcgctccggg cagtggagag gacctcgaag
     5341 cagcttctgc gcctgttgcc cgacgatttg cgcgaggacg atggcgttga tcctcacgcg
     5401 gtatcccggc tcctgctctc ggcctttccg gacagaattt gcaaacggcg ggacgaaggc
     5461 ggcggacgat ttgttcatat gcagggaagg ggggtccgtc tttcggccga aagccacctg
     5521 ggcggaagtc cctatatcgt agccgttgcc gcggatgcag gtgagaaaac tgaaggctac
     5581 gtgcatatcg cggtgccggt taccgaggac ctcatccgga gagaatgcgg acagcgcatc
     5641 gagactgtcc ggagggtgga atgggactgg aaggaaaaca ggatcgctgc agccgttgag
     5701 gagaggctcg gggcactgct gttttcgaag aggccgtttg ttccgacaga cgaggaggcc
     5761 gcgcccattc ttttggatgt ggtgcggact accccttcgg tccttacttt cagcaaggag
     5821 gcgcggcagt ttcaggctcg ggtggggctc ctcggcaggg ttttccccgc ggaagcctgg
     5881 cccgatcttt ccgatgaaca gcttctgacg aaaccgcagg agtggctttt gccatggctc
     5941 ggcggcgttc ggaccggtca ggccct
//
LOCUS       DASSLD010000113         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_835917,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000113 DASSLD010000000
VERSION     DASSLD010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835917"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VFK23_02155"
     CDS             complement(<1..700)
                     /locus_tag="VFK23_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET7317917.1"
                     /translation="MKARVVITGLGAVTPLGNSMERTWKSIINSVSGIGRITKFDASD
                     FSCRIAGELKGFDPSGYMTAKETHRTDPVIQYALAAALMAVEDAGLTITPHSSLRTGV
                     LVGSGRGGVTTSERNLIAFMAKGPRAVSPFYTPMSLVNMASGYISMKLGAKGPCLDVS
                     TACATGTHALGEAMKLIQRGDADVMIAGGAEAALTPLILAGFCQARALSQWNEEPAKA
                     CRPFDRDRDGFVLAE"
     gene            835..1743
                     /locus_tag="VFK23_02160"
     CDS             835..1743
                     /locus_tag="VFK23_02160"
                     /inference="COORDINATES: protein motif:HMM:NF027583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HET7317918.1"
                     /translation="MKKAELTAKTVAELKALAKKKKISLPAGAKKDDIIKVLMASAVR
                     KKTGKPAAKKPVALKRPARRAAVPGPAEKTVKEPAKTPARKWEMPVGAEEPLMAQERV
                     AEAKFYTGPVPDQKSAPSTRPYEELPPGYSEEKIVLMTRDPYVAHAYWEATPARIERE
                     KSWLGWNSKLCIRIYDVTGVQFDGRNALGYYDQEVYEQTGSWYFDVGRPTHSFCADLG
                     LLSPEGKFLTLARSNYVTMPRDGVSDVVDEEWMVVDEEFWKLYGYPEGFPGGLSSQQA
                     QEIVKRRRMQEISSPGMSSREKVKRK"
BASE COUNT          492 a          542 c          604 g          455 t
ORIGIN      
        1 cctcagcgag cacgaagcca tcccggtccc tatcaaaggg cctacacgct ttcgccggtt
       61 cttcgttcca ctgtgacaat gccctggcct ggcagaagcc ggcaaggata agcggcgtga
      121 gtgcggcttc ggccccgccg gcgatcatca cgtccgcatc tccccgctgg atgagcttca
      181 tggcctcacc caacgcatgc gtcccggtag cgcaggccgt cgagacgtca aggcatgggc
      241 cctttgcccc gagtttcatc gaaatatatc ctgaagccat gttcacgagc gacatgggag
      301 tatagaatgg cgatacggcc cgggggcctt tggccatgaa tgcaatgagg ttcctctcgc
      361 tcgttgtgac cccgccgcgg cctgagccta ccagcacacc cgttcggagt gaggagtgcg
      421 gagtgattgt aagtccggcg tcttccaccg ccatcagagc tgctgccaga gcgtactgga
      481 tgacaggatc ggtcctgtgc gtttccttag cagtcatata cccggaggga tcgaatcctt
      541 tcagttctcc tgcgatacgg caggagaagt ccgatgcgtc gaattttgtg atccggccaa
      601 ttccggaaac actgttgatg atcgatttcc atgtcctttc catgctgttg ccgagcggcg
      661 tgaccgctcc aagtcccgtt atgacgacgc gagctttcat ggcgcgcagt ataagacttt
      721 tgtgcttgtt tgtcaaaaac ctgaggagca gagtacaggg aataagtttg acaatgccat
      781 attttgtggt atagtttagt ggagtttaaa tgctgaaaag agagggggtt tgagatgaaa
      841 aaagctgaat taacagcaaa gaccgtagca gaactcaaag ccctggcgaa gaagaaaaag
      901 atatcgctgc ccgctggggc aaagaaagat gatattatca aggtattaat ggcgtccgct
      961 gtgcgcaaga aaaccggaaa acctgccgca aaaaagccgg tggcactaaa gcgcccggca
     1021 cggcgcgcag cggttccggg tcctgccgag aagacggtca aggagcctgc aaagacgccg
     1081 gctcgtaagt gggaaatgcc cgtgggcgct gaggagccgc ttatggctca ggagcgagtt
     1141 gccgaagcca agttctatac aggccccgtt cctgatcaga aatcggcacc ctcaactaga
     1201 ccgtacgagg agcttcctcc tggctatagc gaagagaaga ttgtcctcat gacgcgggat
     1261 ccctacgtgg cccatgccta ttgggaggca accccggcac ggatcgaacg ggaaaagtca
     1321 tggttaggct ggaatagcaa actgtgcata cgtatttacg acgtgactgg cgtgcaattc
     1381 gacggccgca atgctctggg ctattatgac caggaggtct acgaacagac cggcagctgg
     1441 tattttgatg tcggaagacc aacccattca ttctgtgccg accttggcct gctttctccc
     1501 gaaggtaagt tcctcacgct cgcgcggtcg aactatgtca ctatgccgag ggatggcgtg
     1561 tccgatgttg tcgatgagga gtggatggtt gtggatgagg agttctggaa actgtacggc
     1621 taccccgagg gtttcccggg ggggctttcg tcgcagcagg cccaagagat cgtgaaacgc
     1681 agacgcatgc aggagatatc ctctcccggc atgtcctccc gtgagaaggt gaagagaaag
     1741 tagcggcatg cagcagggtt tcgtttccgg aaggccttgc cttttgtaac ggtggcagat
     1801 agtccatcct tcgttggatg gacttttctt ttgtaatttc ccggattacc agagaagagt
     1861 actttttatg agctggaaca ccccgcggaa caataaaaac gagcgagcaa gttccggctc
     1921 ggaaggcgat ggaggccgaa tggaaaaagg ttatctagca ctggtactgc acagccatct
     1981 gccctttgta cgacatcctg aacatgacca gttcctcgaa gaggactggc tctacgaagc
     2041 gattaccgaa acatatatcc ctctcatcaa cgtgttcgac ggcctggtga acg
//
LOCUS       DASSLD010000114         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1456051, whole genome shotgun sequence.
ACCESSION   DASSLD010000114 DASSLD010000000
VERSION     DASSLD010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456051"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VFK23_02165"
     CDS             <1..1195
                     /locus_tag="VFK23_02165"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HET7317919.1"
                     /translation="KMDAEDPLFILYTSGSTGKPKGVLHTTAGYLLYTNLTFKWIFDY
                     HEEDIHFCTADIGWVTGHSYIVYGPLSAGATSLMFEGIPTYPNPGRFWDIVDKHRVSI
                     FYTAPTAIRALMKEGEKWVSKHDLSSIKVLGTVGEPINPEAWMWYHKNIGREKIPIVD
                     TWWQTETGGILITGLPGAITAKPGSATRPFPGVVPQVVKEDGSPAGVNEGGYLCINKP
                     WPGMLRGTYGDPENKRIKEVYFSRFPGRYFTGDGARIDEDSDYWLMGRVDDVINVSGH
                     RLGTAEVESALVSHEAVAEAAVVGYPHDIKGEGIYVYVTLKDGQKPSDELKKILAGHV
                     RSVIGPIATPDKLQFAPGLPKTRSGKIMRRILRKIAHGQIEDLGDTSTLADPSVVESL
                     VKGKQ"
     gene            1393..>2017
                     /locus_tag="VFK23_02170"
     CDS             1393..>2017
                     /locus_tag="VFK23_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HET7317920.1"
                     /translation="MTVNKRINLMREFSVPLVSGVVIALVWANKAPESYHHFVHGNFL
                     GSLSFHFLTNDVFMVIFFGIAAVEITQSFLPGGDLYPIKKSVNPLLSTAGGVIGPALV
                     YLGLNNLIGTPELQRGWGIPTATDIAFAWLVARMIFGSNHPAIPFLLLLAVADDGIGL
                     AIIAVFYPDPNFPVEPSWLVLTLAGMAAALFLRTQGVKSYWPYIIAGG"
BASE COUNT          420 a          612 c          589 g          396 t
ORIGIN      
        1 gaagatggac gcggaagacc cgctgttcat tctctacacg tcgggttcga cgggcaaacc
       61 caagggggtt ctccacacga cggcaggcta cctgctgtat acgaacctga ccttcaagtg
      121 gatcttcgat tatcacgagg aggacatcca tttctgcacg gcggacatcg ggtgggtcac
      181 cgggcacagc tacattgtct acgggccgct ctccgcgggg gcaacgagcc tgatgttcga
      241 gggcatcccg acgtatccga atcccggaag gttctgggac atcgtggaca agcaccgggt
      301 cagcatcttc tacactgcgc ctacggccat ccgagccctc atgaaggaag gcgagaaatg
      361 ggtgtcgaaa cacgacctgt cgtccatcaa ggtcctcggc accgtgggag agccgatcaa
      421 ccccgaggcg tggatgtggt accataagaa catcggcagg gagaagatcc ccatcgtgga
      481 tacctggtgg cagaccgaga ccggcggcat cttgatcacc gggcttcccg gcgctattac
      541 ggccaaacca ggttctgcga cgagaccctt cccgggggtc gtgccgcagg tggtaaaaga
      601 ggacggttct cccgctggcg taaacgaggg cggctacctc tgcatcaaca agccctggcc
      661 cggcatgctc cggggcacct acggagaccc ggagaacaag cgcatcaagg aggtctattt
      721 cagccggttc ccgggcagat actttacggg tgacggggcg cgcatcgacg aggacagcga
      781 ctactggctc atgggccgcg tcgatgacgt catcaacgtc agcggccacc ggctcggcac
      841 cgccgaggtc gagtctgcgc tggtcagcca cgaggcggtc gcggaagccg ccgtggtcgg
      901 ctacccgcac gacatcaagg gcgagggcat ttacgtctat gtgaccctga aggacggtca
      961 gaagccctcg gacgagctca agaagatact cgccggccac gtccgctcgg tcatcgggcc
     1021 gatcgcgacc ccggacaagc tccagttcgc tcccggactc cccaagacgc gcagcggcaa
     1081 gatcatgcgc cggatcctgc gcaagatcgc ccacggccag atagaggatc tcggcgatac
     1141 ctccacgctc gccgacccgt cggtggtgga gagcctggta aagggcaaac agtaacgagc
     1201 caagttcttt ctgttgcagc gcgcaaggcc ggaaaacatc ccggcctttt ttttcgaact
     1261 tttcccgaaa atcgtcaaga ggctttccca actgccggag caggccgcct gaaaacgacg
     1321 ctttgtcatt gacatcccct ccggtcgctg ctagtatccc gccaatcagc gagattcaag
     1381 acatcaaggg gtatgacagt gaacaaaaga atcaatctga tgcgcgaatt ttccgtaccg
     1441 ctcgtaagcg gggtcgtcat cgcgctggtc tgggcaaaca aggcgccgga gagttatcat
     1501 catttcgtgc atggaaactt cctcggttcg ctcagttttc acttcctgac caatgacgta
     1561 ttcatggtca tttttttcgg catcgctgcc gtggaaatca cccagagttt ccttcccgga
     1621 ggggacctct accccatcaa aaagtctgtc aatccgctgc tctcaaccgc cggcggtgtt
     1681 atcgggcctg cgctggtata tctggggctc aacaacctga tcggtacgcc cgagttgcag
     1741 agagggtggg gaatccccac ggccacggac atcgcgttcg catggctggt tgcgaggatg
     1801 atcttcggca gcaatcaccc agcgattccc ttccttcttc tgctcgcggt ggcagacgac
     1861 ggtatcggac tggccatcat cgctgtattc tatccggacc cgaattttcc cgtggagcct
     1921 tcctggcttg tacttacgct tgcaggcatg gccgcggcct tgttcctgcg gacgcaagga
     1981 gtcaaatcct actggcccta tatcatcgct ggcggcg
//
LOCUS       DASSLD010000115         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_650876,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000115 DASSLD010000000
VERSION     DASSLD010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650876"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VFK23_02175"
     CDS             <1..641
                     /locus_tag="VFK23_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317921.1"
                     /translation="ADGVIFVADSQRAMMGANLESLTNLRDNFAETGSDLTDFPMVIQ
                     YNKRDLPDIMSIDEMNETLNPRNIPFYEAIATTGDGVLKTFTAMSKLVLQDMQRSPER
                     HNFMLRDIIGKAEKRTLRPKEQPKEQLPYEPSMPGAPMAEAKPEMAALEEAVDAGMKV
                     NLGKPFMEDRQIILPFDVLTMDREDQFQLRVAIDMESPLSKRFKVVVKNFRK"
     gene            750..1439
                     /gene="ispD"
                     /locus_tag="VFK23_02180"
     CDS             750..1439
                     /gene="ispD"
                     /locus_tag="VFK23_02180"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET7317922.1"
                     /translation="MSKVTALIPAAGMGRRMGKAVAKQFLPLGDMPMLAHTLLMFQRA
                     SEIDEIIPILSKEDMEICLKDIIEQYHITKVKTLVVGGKERQDSVQNGLQKLEKDASV
                     VLIHDGVRPFVTHDMIKDAVDHAKKGECVAVGVPLKDTVKEVNDKGMVVHTLERSRLW
                     AIQTPQAFPVKILRRAYEDSYKQNLYGTDDATLVERTGGKVRVIMGSYDNIKITTPED
                     LLLAEEILKRR"
     gene            1441..1917
                     /gene="ispF"
                     /locus_tag="VFK23_02185"
     CDS             1441..1917
                     /gene="ispF"
                     /locus_tag="VFK23_02185"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET7317923.1"
                     /translation="MRIGTGYDVHRLVEGRKLMIGGVDIPSERGLLGYSDADVLLHAI
                     CDALLGAAGLGDIGRHFPDTAPKYKGISSLSLLTEVYRLLTEGGFRVNNIDATIVAER
                     PKMAAHIPAMVANIASAVNADRSAVNVKATTTEGLGFAGRGEGIAAYAVCTIEHST"
BASE COUNT          508 a          533 c          612 g          393 t
ORIGIN      
        1 gcgctgatgg cgtgatcttt gtggcggact ctcagcgcgc catgatgggc gcgaacctcg
       61 aaagcctgac gaatctccgc gacaatttcg ccgaaacggg aagtgacctg actgacttcc
      121 ccatggtgat ccagtataac aagcgcgacc tgcccgatat catgtccatc gatgaaatga
      181 acgagaccct gaacccgcgc aatatcccgt tctacgaggc gattgcgaca acaggtgacg
      241 gcgtgcttaa gaccttcacc gccatgtcca aactcgtgct tcaagacatg cagaggtctc
      301 ctgaacgcca caacttcatg ctccgcgaca ttatcgggaa ggcagaaaag aggacgctac
      361 ggccgaagga acaaccgaaa gaacagcttc cctatgagcc ctcaatgccc ggagcgccca
      421 tggcagaggc caaacccgag atggctgccc ttgaagaggc cgtggacgca gggatgaagg
      481 tgaatctcgg aaagccgttc atggaagacc ggcagatcat ccttcctttt gatgtcctga
      541 ccatggaccg ggaagaccag ttccagctcc gcgtcgctat cgatatggaa tcaccgctgt
      601 cgaagcggtt caaggtagtc gttaagaact ttaggaaatg atcggttgac attgaagacg
      661 cggatttatc aagaccgatg cagcagggaa atcgatagca taggaaatca gtgtcacctg
      721 tttctggaga cccccgctgt gaggttacta tgtcaaaggt aacagcgctt atacctgcag
      781 ctggcatggg caggagaatg ggaaaggccg tggcaaagca gttcctgccg cttggtgaca
      841 tgcccatgct ggcgcacacg ctcttgatgt tccaacgcgc gtcggagatc gacgagatca
      901 taccgatcct ctcgaaggaa gatatggaga tctgccttaa ggatatcatc gaacaatacc
      961 atatcaccaa ggtaaagacc ctggtcgtgg gaggcaagga acggcaggat tccgtgcaga
     1021 acgggctgca gaagcttgag aaggacgcgt cggtcgtgct catccacgac ggggtgaggc
     1081 ccttcgtcac gcacgatatg atcaaagatg ctgttgatca cgccaagaag ggagagtgtg
     1141 ttgccgtcgg cgtgccactc aaggataccg taaaagaagt gaacgataag ggcatggtcg
     1201 tccacacgct ggagcggagc aggctctggg ccatacagac cccccaggct ttcccggtta
     1261 agatcctcag acgtgcttac gaagattcgt acaagcagaa cctctatggg acggatgatg
     1321 ccacgctcgt cgagcggaca ggcggcaagg tccgagtgat catggggagc tacgacaaca
     1381 ttaaaatcac tacgcccgag gacctcctcc tggcggaaga gatcctcaag agaaggtagt
     1441 atgagaatcg gcacgggtta tgatgtacac cgcctcgttg aaggaaggaa gcttatgatc
     1501 ggcggcgtgg atatcccctc cgaaagagga ctgcttggct actccgacgc ggacgttctg
     1561 ctccatgcaa tctgcgatgc gctgctcggc gccgcggggc tcggcgacat cggacggcat
     1621 ttcccggaca cggctccgaa gtacaagggc atatcgagtc tctccctgct taccgaggtg
     1681 tatcggctgc tgaccgaggg cggttttcgc gtgaacaaca ttgatgccac aatcgtggcg
     1741 gagcggccga aaatggcagc ccatatcccg gcaatggtcg cgaacatcgc atcggcggtc
     1801 aatgcagacc ggtctgcggt caatgtcaag gccacgacaa ccgaggggct cgggttcgca
     1861 ggcaggggcg aaggaattgc ggcatatgcc gtttgtacga tcgagcattc gacgtgagcc
     1921 gttgcatcgg aaggtgctag aggaggtgaa acatgcggta taccatcatc atgggtgttc
     1981 ttttggccgc ggttttctta atcggtgtgc aggcaagggc gcaggaggga agcagcgagc
     2041 agaaaa
//
LOCUS       DASSLD010000116         4481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_151482,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000116 DASSLD010000000
VERSION     DASSLD010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151482"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..375)
                     /locus_tag="VFK23_02190"
     CDS             complement(169..375)
                     /locus_tag="VFK23_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258239580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(337..339),aa:Sec)
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HET7317924.1"
                     /translation="MADKVMIFGKAGUPYTEKARSAYGAQAVYFDVKADSAKLEEMLM
                     LSRGERKVPVIVEDGKIAVGYGGS"
     gene            587..1180
                     /locus_tag="VFK23_02195"
     CDS             587..1180
                     /locus_tag="VFK23_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7317925.1"
                     /translation="MPARDDDAVLVSASQQGDLNSFETLVARHQKRMFNIAFRLTGNY
                     DEACEVVQDAFVSAYKNIRTFRGEAKFTTWLTTITVNLSKNRLKQIQSRRRHEAYSLD
                     ESVRTDDGEMAVDPPSHEPSVLDRLEKQDIRDRVQDCIKALEPDFREILVLRDLQDFS
                     YGEIGGMLAVPEGTVKSRLFRARELVRDCLKRFMGEL"
     gene            1180..2214
                     /locus_tag="VFK23_02200"
     CDS             1180..2214
                     /locus_tag="VFK23_02200"
                     /inference="COORDINATES: protein motif:HMM:NF021550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2275 domain-containing protein"
                     /protein_id="HET7317926.1"
                     /translation="MDHNDIRHKLSEYLDGSVTAEEKSLIEEHLKTCAPCSDALQELR
                     KTVEHIKSVEEVEPPAWMTQKIMANVRAEAEEKKGLFRRLFSPLAVKLPVQAVAVLFL
                     AVTAYYLYQNINPAEKYAKAPSEKSEAAKPASPMLSPQPEPKIRREPAAPAAKAPQAP
                     EYKALDMKMEYEKPAPPVPTGKIEAPGPAPAKPAEQPLPAKREEAAGKRTAESQAGAS
                     SATQEQASAQALRPETKLGSDAIERRAVKAKSLEKAGLVISLMVKDVEAAAGEVERLI
                     TKLGGSITMKEDLEAKKTFGVAIDVQKLQELRSNLKLIGEVRDDAVEPALHDKRVELK
                     IEIVASSTQP"
     gene            complement(2272..2760)
                     /locus_tag="VFK23_02205"
     CDS             complement(2272..2760)
                     /locus_tag="VFK23_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020586402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HET7317927.1"
                     /translation="MDVCPCGSTMEYAECCRPLISGEKRAETAEQVMRARYSAYVMKE
                     MEYLRTSLHPDHRSDYDEKGSRAWAERTAWHGIEIHDTVKGGPDDSEGQVEFTASFTE
                     NGIRQEYRERSTFRKDHGIWYFETGKVLPPKPVVRTAPKAGRNDPCPCGSGRKFKKCC
                     GQ"
     gene            2993..4450
                     /locus_tag="VFK23_02210"
     CDS             2993..4450
                     /locus_tag="VFK23_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HET7317928.1"
                     /translation="MKSKYVVVLAVLFLVSIGYCPVSSAGKKEAKTDAATSTLDDQTG
                     VALTVYNVNLGLVKDQRQIKLGKGVSDLRFMDVASQIMPTSVHIKSLIDPETLRVLEQ
                     NYEYDLLNPQKLLDKYVGREVKLYTKNPHTEREEVVTATLLSNNGQPIFKIGDEITCG
                     HPGRIIFPGVPEDLNAKPTLVWMIENSLPSAQRIEASYLTSGINWRSDYVVTLNEKDD
                     KADLSGWVTIDNHSGALYKNAKLKLVAGDVNRVRDEHEYRDKMMRVAEVAAAKAPAPQ
                     FKEDSFFEYHIYTLERPATVKDNQTKQISLVSADNIPVKKELLYSGANYYYYSRYGEA
                     MSNQKVGVFVEVANKKENNLGIPLPKGTVRVYKADKEGSLQFVGEDSIDHTPKDEKVR
                     IKLGEAFDVVGSRKQTDWKKIAYDTYEAAFEISLRNHKKEDVVVKVVEPIPGDWTMLA
                     SSHAYKKSEAFTAEFNIPVPKDQEVKLTYRVRMRY"
BASE COUNT         1096 a         1253 c         1235 g          897 t
ORIGIN      
        1 tggatatcaa cgatgaggag cacggcgtct tctctgttca gaataaattt atccggcccc
       61 atccgtactt catctccttg ataacgatcc cttttcgatt ttattccgca ttccgcattc
      121 caaattccgc attgattcat aaccggcaac tgccggccct cacacccctc aggacccgcc
      181 gtagccgacg gcaatcttcc cgtcttcgac gatgaccggg accttccgct cgcccctcga
      241 gagcatgagc atttcctcca gtttcgcact gtccgccttt acatcgaaat agaccgcttg
      301 agcgccatag gccgacctgg ccttttccgt gtacggtcag cctgccttcc cgaatatcat
      361 caccttgtcc gccatggagt gcctcctcga gtctttatta ttgataacgc cgtaataagt
      421 cggatttagt ggattttgac tattacgaga tcattattat tgaatactta ataataatat
      481 cattttacgg gtgcaaaagt aaactcgttg agatagaatc tgaatcgtgc aatcaaacgg
      541 aacaaattct ccggcacgct tgtcctatgg aacagaggga gaaggcatgc ctgctcggga
      601 cgacgatgca gtcctcgtct cggcctcgca acagggagac ctgaactcct tcgaaacgct
      661 cgtcgccagg caccagaaga ggatgttcaa catcgcattc cggctgaccg gcaattacga
      721 cgaagcctgc gaggttgtcc aggacgcctt tgtttccgca tacaaaaaca tccggacgtt
      781 ccggggcgag gcgaagttca cgacatggct gacgaccatc accgtgaacc tgtcgaaaaa
      841 ccggcttaaa cagatacagt cccggaggcg gcacgaagcg tactcgctgg acgaatccgt
      901 ccggaccgac gacggagaaa tggcggttga tccgccgtcg cacgaaccgt cggttttgga
      961 ccggctcgaa aagcaggaca tacgggacag ggtgcaggac tgcatcaagg ccctggagcc
     1021 ggactttcgc gagatattgg tccttcggga cctgcaggat ttttcctacg gagagatcgg
     1081 gggcatgctc gcagtcccgg aaggcacggt caaatcccgg ctgttccggg ctcgtgagct
     1141 ggtcagggac tgcctcaaac gattcatggg agaactttga tggaccacaa cgacatccgc
     1201 cataaactgt ccgaatacct ggatggatcg gttacggccg aagagaaatc gttgatagaa
     1261 gagcatctga aaacctgcgc tccgtgcagc gacgccctcc aggaactccg gaagaccgtc
     1321 gagcacatca agtccgtcga agaagtggag cccccggcat ggatgacgca gaagatcatg
     1381 gcaaacgtgc gcgcagaggc ggaagagaag aagggcctgt tccggagact gttctctccc
     1441 ctcgccgtca aattgccggt acaggccgtg gccgtcctgt tccttgccgt gaccgcttat
     1501 tacctctatc agaacatcaa tcccgcagaa aagtacgcca aagcgccgtc tgaaaaatcc
     1561 gaggcggcaa aaccggcctc cccgatgctt tctccgcaac ccgagccgaa gatacggcgt
     1621 gagcctgccg ctccggccgc caaggcgccg caagcacctg agtacaaggc cctcgacatg
     1681 aagatggaat acgagaagcc tgcgccgcct gtgccgaccg gcaagattga ggcgcccgga
     1741 cctgcaccgg caaaacctgc ggagcagcca ttacccgcaa aacgggaaga ggcagcggga
     1801 aagcgcacag ccgagtctca ggccggcgcg tcttctgcaa cgcaggagca ggcatccgct
     1861 caggcattac gtccggaaac gaagttggga tcggatgcga tagagcggcg ggcagtaaag
     1921 gcaaagtcgc ttgaaaaggc cggtctcgtg atcagcctca tggtaaagga cgtcgaagct
     1981 gctgccgggg aagtcgaacg gcttatcacg aagctcggcg gatcgatcac gatgaaagag
     2041 gacctcgaag cgaaaaaaac attcggggtc gcgatcgatg ttcaaaaatt gcaggagctc
     2101 aggagcaatc tgaagctgat cggagaggtc agggatgacg ccgtcgagcc ggcattgcat
     2161 gacaagcggg ttgaattgaa aatcgagatt gtggcgagtt caactcagcc gtgatgaata
     2221 aaagcagctc ctgactctgt gatctctgtg tggtccaatg ccttttcagg tttattgtcc
     2281 gcagcatttc ttgaacttcc tgccgctgcc gcaggggcag ggatcgttcc tgccggcctt
     2341 aggagccgtc cggacgaccg gcttgggagg aagaactttc cctgtttcga aataccagat
     2401 gccgtggtcc ttcctgaagg tggacctttc gcggtattcc tgcctgatac cattctccgt
     2461 gaacgatgcg gtaaattcaa cctggccttc gctgtcatcc ggcccgcctt ttaccgtgtc
     2521 gtgaatttca atgccgtgcc acgctgtccg ctccgcccag gcgcggcttc ctttttcatc
     2581 atagtccgac cggtggtccg gatgcaggga cgtgcggaga tactccattt ccttcatgac
     2641 ataggcgcta taccgggcgc gcatgacctg ctccgccgtc tcagcacgtt tctcgccgga
     2701 aatcagcggc cggcagcatt ccgcgtactc catagtggaa ccgcagggac aaacatccat
     2761 tcgtcgctcc tcttttaagc catgattatg gggacgaacc acggtagcat agatcctgcg
     2821 cagggtcaag aaaatgtctg acaaagaact tattaatcgg gtggggtaac gcagcctgat
     2881 cgctatcccg ttggactgcg atcggagacc cccttttcgt cgggaacaat ttcctcccct
     2941 cctttgtcta atggctaaaa gaagtccaat ccattgatgg aggaggcctt tcatgaagtc
     3001 caaatatgtt gttgtcctgg cagtgctgtt cctggtaagc atcggctatt gtccggtttc
     3061 atcggcaggg aaaaaagaag cgaagactga cgcggcaaca agcacgctgg atgaccagac
     3121 cggcgtggca ctcaccgtct acaacgtcaa tctcggcctg gtcaaggacc agcggcagat
     3181 caagctcggg aaaggcgtca gcgatctgcg gttcatggac gtggcgtcgc agatcatgcc
     3241 tacgagcgtc cacatcaagt ccctcattga tcccgaaacc ctgcgcgtgc tggagcaaaa
     3301 ctacgagtac gatctcttga atcctcaaaa gcttctcgac aaatacgtcg gcagggaagt
     3361 caagctctac accaagaatc cccatacgga gcgcgaagag gtcgtcaccg ccaccctcct
     3421 gtcgaacaac ggccagccca tcttcaagat cggagacgag atcacctgtg gccatccggg
     3481 caggatcatc ttcccgggcg tgcccgagga cctgaatgcg aaacccacgc ttgtctggat
     3541 gatcgagaac agcctgccct cggcacagag gatcgaggcg tcatacctta ccagcgggat
     3601 caactggagg tccgactacg tggtgaccct gaatgagaaa gacgacaagg cggacctttc
     3661 gggctgggtc actatcgaca accacagcgg cgccctgtat aagaacgcga agctcaaact
     3721 cgtggccgga gatgtgaacc gggtgaggga tgagcacgaa tacagggaca aaatgatgcg
     3781 tgttgccgag gtagccgcgg caaaagctcc cgctccccag ttcaaggaag attcattctt
     3841 cgagtaccat atctataccc tcgaacgtcc tgccacggtc aaggacaacc agaccaagca
     3901 gataagcctg gtttcagcag acaatatccc ggtcaagaaa gagcttctct actccggggc
     3961 gaattattat tactacagcc gttacggcga ggcaatgtcg aaccagaagg tcggcgtgtt
     4021 cgtcgaggtc gcgaacaaga aagagaacaa tctcggcatc cccctgccca aaggtacggt
     4081 aagggtttat aaggcggaca aggaaggaag cctgcagttc gtgggcgagg actcgatcga
     4141 ccacacgccc aaagacgaga aagtcaggat caagctcgga gaagcctttg acgtggtggg
     4201 aagcaggaag cagacggact ggaagaagat cgcctacgat acctacgagg cggctttcga
     4261 gatttcgctc aggaaccaca agaaggagga cgtggtcgtg aaggtcgtgg agcccattcc
     4321 cggcgactgg accatgctcg cctcgtcgca cgcctacaaa aaatccgagg cctttaccgc
     4381 cgagttcaat attcccgtgc cgaaagacca ggaagtgaag ctcacctacc gggtgaggat
     4441 gaggtattaa agcagttcgg agtttacagt tcggagttcg g
//
LOCUS       DASSLD010000117         5375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1520874, whole genome shotgun sequence.
ACCESSION   DASSLD010000117 DASSLD010000000
VERSION     DASSLD010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520874"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VFK23_02215"
     CDS             <1..998
                     /locus_tag="VFK23_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HET7317929.1"
                     /translation="GTAVKVDERGRVEWDQKTQMSSAKGVFLSGEVVTGPGSAIAAVA
                     NGHRAALAMHLYCQGETIEGKLPAQEKEKIAQMPADLVEKIAKEPRVKIKHLAPEVRC
                     DTMVHFEIGYDEAEALKEAGRCRGCGGGAVVDKNKCMACLTCMRICPYGAPVVTSHSE
                     IRPEYCQACGLCAPECPGEAISMVSYDIREHRNNMPSLLGKTDPKRQEPVIVAFMCTH
                     HVGVLGFETPKNIKTVPVHCTSRIDVLDILKAVESGADGVAVVRCGDATCKYRNIEPR
                     VNARVRRAQELVGALGIDKGRIEILSTSHGNGGNPYAAVCTEFSEKVKKIGLRTK"
     gene            1037..1738
                     /locus_tag="VFK23_02220"
     CDS             1037..1738
                     /locus_tag="VFK23_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HET7317930.1"
                     /translation="MIVGSQKPIDEIWSMVKDFKKVLVFGCNTCVAICHAGGEKEAET
                     IASLIRMRAAQEGKEMTVGHLGVMRHCEPEYFDPVMDEVKKYDLVLSTACGVGVNFLS
                     DRTGTIPVYPGINTSFYGAVEAPGFFKELCAGCGNCIVHLTGGLCPIARCSKTLMNGP
                     CGGTNKGKCEVSNEIDCIWYLIVERMKKLGTLEKLSEIQPARNWSTSRDGGPRRISLE
                     HIREFEEKEEAKAGK"
     gene            1872..2843
                     /locus_tag="VFK23_02225"
     CDS             1872..2843
                     /locus_tag="VFK23_02225"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HET7317931.1"
                     /translation="MKSGSNLEKVIASGKPAVTAELGPPMSADGHEVVKKAQLLKGFC
                     DAANITDCQTAVVRISSIAAATIAHQNGLEPVMQMTCRDRNRIAIQADLLGAAALGLK
                     NCLCLAGDHQKFSAAGKLKGHPGAKNVYDVDTCQLVGILKKMRDEGKQEGGDPLEVAP
                     KFFVGASWTPMADPIDFRPINLMKKVNAGADFIQTQGIYDMEGFRAQMAKIRNMGLHE
                     RTAILAGIIVPKSAMMLKYMDSSVAGVSVPKPLIDRMNKAKAAAGEDKKKAKELQEQE
                     GIKITIELIHQALETPGVKGVHIQAIEWESAIEGIVKQAGLYPRPQV"
     gene            2912..3712
                     /gene="panB"
                     /locus_tag="VFK23_02230"
     CDS             2912..3712
                     /gene="panB"
                     /locus_tag="VFK23_02230"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET7317932.1"
                     /translation="MPKITIHDIYKKKAEGRKITMLTAYDFPTAQIVDQAGIDMILVG
                     DSLGNVVQGVANTLPVTMDEMIYHTKMVSRAAQSALVVGDMPFLSYQTHKAEAIRNAG
                     RFLKEAGAEAIKLEGGTQMGETIRAIVDAGIPVVAHIGLTPQYVHMLGGFKVQGKDEA
                     AREKILADARAVQDAGAFSVVLEAMPASLAKEIREMLHIPTIGIGAGPDCDGQVLVLH
                     DILGLFDRFTPKFVKKYANLKDQALKAVREYKQEVESGSFPSQEHSFK"
     gene            3758..4468
                     /locus_tag="VFK23_02235"
     CDS             3758..4468
                     /locus_tag="VFK23_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET7317933.1"
                     /translation="MSLFCLRAKTISDAWFQLIYNIFDHSYTQKIQKGSFEKEQYRLQ
                     YAGLAVFIEHPYHDIVPVIPPALGIPSPTTMEYIDDYFANYLMDPGLAENETYKYASR
                     IQHPMPKGGTQLDRAIAMLKETPLTNQAIVEIGSPDDHDVCIGNDGKLDPPCLRLLDF
                     KAVPAGDELVLSLSCYFRSWDLWAGFPTNLGGLELLKRYVASEAGLKNGPIYAYSAGA
                     HIYGYQEEIARIRTLRQK"
     gene            4745..>5375
                     /locus_tag="VFK23_02240"
     CDS             4745..>5375
                     /locus_tag="VFK23_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HET7317934.1"
                     /translation="MGFTIAVAGKGGTGKTSLCGLTIRYLTEKKKGAVLAVDADANAN
                     LNDVLGVRIHATVGKLRESSLEAIKSTAPRPGGMSMEQLFDYQIQQALAESKGFDLLT
                     MGRPEGAGCYCAANNIIRKYMDKLADSYPYTVMDNEAGLEHLSRRTTQDTDLLMIISD
                     SSVRGVMTAGRIGELVKELNLNVKRSVLIVNRVQDGTSDDPSLLQVIRQQ"
BASE COUNT         1308 a         1446 c         1615 g         1006 t
ORIGIN      
        1 aagggactgc cgtcaaggtg gatgagaggg gccgggtgga atgggaccag aagacccaga
       61 tgagcagcgc aaagggcgtg ttcctgtccg gcgaggtcgt gaccgggccg ggttctgcca
      121 ttgccgccgt ggcaaacggc catcgtgcgg cgcttgcgat gcatctttac tgccagggag
      181 agacgattga agggaagctc cccgcacagg agaaggaaaa gatcgcacaa atgcccgcag
      241 acctggtaga gaagattgct aaggaaccgc gggtaaagat caagcatctc gctcccgagg
      301 tccgctgcga caccatggtc catttcgaaa tcggctacga cgaggccgag gctcttaagg
      361 aagcgggccg gtgccggggc tgcggaggcg gcgcggtcgt ggacaagaac aagtgcatgg
      421 catgcctcac gtgcatgcgc atctgccctt acggcgcgcc ggtggtaacc tcacattccg
      481 agatcaggcc cgagtactgc caggcctgcg ggctgtgcgc gcccgaatgc ccgggcgagg
      541 cgatcagcat ggtgagctac gatatcaggg aacaccgcaa caacatgccc tcactcctcg
      601 ggaagactga tccaaagcgg caggagccgg tcatcgtcgc ctttatgtgt acgcaccatg
      661 tgggcgtctt gggattcgaa acgccgaaga atataaagac cgtcccggtc cattgcacga
      721 gcaggatcga tgtcctcgat atcctgaagg ccgtggaaag cggtgccgac ggcgttgccg
      781 tcgtccggtg cggggacgcg acgtgcaagt accggaacat cgagccgcgc gtgaacgcac
      841 gcgtcaggcg ggcgcaggaa ctggtcgggg cgctggggat cgacaaaggc cgcatcgaga
      901 tcctctccac ctcccacgga aacggcggga acccctatgc tgcggtgtgt acggagtttt
      961 cggagaaggt gaagaagata gggttgagga cgaagtaatc gtttttcgat atcgatacaa
     1021 taaggagctt tgaactatga tcgttggatc acaaaaaccg attgacgaaa tctggagcat
     1081 ggtaaaggac ttcaagaagg tcctggtgtt cggctgtaat acctgtgtgg ccatctgcca
     1141 cgcgggcggc gagaaggagg ccgagaccat cgcctcgctc atccgcatgc gcgcggcaca
     1201 ggaaggcaag gaaatgaccg tgggtcacct cggagtgatg cgccactgcg agccggaata
     1261 cttcgatccc gtgatggacg aagtgaaaaa gtacgacctc gtgctctcca ctgcctgcgg
     1321 ggtgggcgtg aactttctgt ccgaccggac gggcacgatc ccggtctatc ccggcatcaa
     1381 cacctcattc tacggcgcgg tggaggcgcc gggcttcttc aaggaactct gcgccggctg
     1441 cggcaattgc atcgtgcacc tgacgggcgg cctctgcccc atcgcgcgct gctccaagac
     1501 gctcatgaac ggaccctgcg gcggtacgaa caaaggaaaa tgcgaagtct cgaacgagat
     1561 cgactgcatc tggtacctga tcgtcgagcg gatgaagaag ctcggcaccc tggaaaagct
     1621 ctcggagatc cagcccgcca ggaactggtc cacctcgcgg gacggcggtc cgcgccggat
     1681 atccctggag cacatccggg agttcgagga gaaggaagaa gcaaaggccg gaaaataatc
     1741 aaaccttatt ggacgctgat aaacgcagag cgcttcgcgc tacgcagata aaatattgaa
     1801 cgtacggaat tctcatctgc gtgcatcttc aaaaatctgc gtccacagat tttaagtttg
     1861 gaggaacaat aatgaaaagc ggaagcaacc tggaaaaagt tattgcgagc ggaaaaccgg
     1921 cggtaacggc ggagctcggg ccccccatga gcgccgacgg ccacgaagtc gtcaagaagg
     1981 cgcagctcct taagggattt tgcgacgcgg cgaatatcac ggactgccag accgcggtcg
     2041 tccggatatc gagcatcgcg gccgccacga tcgctcacca gaacggcctc gagccggtca
     2101 tgcagatgac ctgcagggac cggaaccgca ttgccatcca ggcagacctc ctgggcgctg
     2161 cggcgctcgg cctcaagaac tgcctctgcc tcgcaggaga ccatcagaaa ttcagcgcag
     2221 cgggaaagct caagggccat ccgggtgcga agaatgtgta cgacgtcgat acctgccaac
     2281 tggtcggcat cctgaaaaag atgcgcgacg agggcaagca ggagggcggc gaccccttgg
     2341 aggttgcccc gaaatttttt gtcggcgctt cctggacccc aatggccgat cctatcgatt
     2401 tccggccgat caatctcatg aagaaggtga acgcgggtgc ggatttcatc cagacccagg
     2461 gcatttatga tatggaaggg ttccgtgcgc agatggccaa gatccggaac atgggtctcc
     2521 atgagcgtac agccatcctt gccggtatca tcgtgccgaa gagcgcgatg atgctcaagt
     2581 atatggactc ctccgttgcc ggtgtgtcgg tgccgaaacc cctcatagac cggatgaaca
     2641 aggcgaaggc ggcggccgga gaggacaaga agaaggcaaa ggagcttcag gaacaggaag
     2701 gcatcaagat cacgatcgag ctcatccatc aggcattgga gaccccgggc gtcaagggcg
     2761 tgcatatcca ggccatcgaa tgggaaagcg ccatcgaagg gatcgtgaag caggcagggc
     2821 tgtatccgag gccgcaggtg taagcgcaac aaccggagta acgagtccgg agctcggggc
     2881 gaactaatca ccgccccaca tggactggcc aatgccgaaa attacaatcc acgacattta
     2941 caagaaaaag gccgaaggca ggaagatcac tatgctcacg gcctatgact tccccacggc
     3001 ccagatcgtt gaccaggcgg gtatcgacat gatcctcgtg ggggattcgc tcggcaatgt
     3061 ggtgcagggt gttgccaata cgcttcccgt caccatggac gagatgatct atcacacgaa
     3121 aatggtctct cgtgccgcgc aatcagctct ggtcgttggc gatatgccct ttctttccta
     3181 tcagacgcac aaggccgaag ccatccggaa cgcgggacga tttttgaaag aggccggggc
     3241 tgaggcgata aagctcgagg gtggaacgca gatgggtgag acgatcaggg ccatcgtaga
     3301 tgcgggtata ccggtcgtgg ctcatatcgg ccttaccccg cagtacgttc atatgctggg
     3361 cggattcaag gttcagggta aagacgaggc cgcccgggag aaaatcctcg cagatgcgcg
     3421 tgcggtccag gatgcgggtg cattctccgt tgtgctcgag gcaatgccgg cgtcgctggc
     3481 aaaggaaatc cgtgaaatgc tccatatccc gaccattggg atcggcgccg ggccggattg
     3541 tgacggccag gtgctcgtgc tgcatgatat ccttgggcta ttcgaccgct ttacgcccaa
     3601 gtttgtcaaa aagtacgcca acctgaagga ccaggccttg aaggccgtca gggaatacaa
     3661 gcaggaagtt gaatccggaa gctttccttc ccaggaacac agcttcaaat aatcaaaaag
     3721 gtccacgcgg atagcggttg tcgatgagga gaatctcatg tcccttttct gtttacgggc
     3781 caagaccata tcagatgcat ggttccagct gatttataat attttcgacc actcatatac
     3841 gcagaaaatt cagaaaggct cctttgaaaa agaacaatac cggcttcagt atgcgggact
     3901 cgccgtgttc atcgaacatc cctaccatga catcgtcccc gtcattccac cggccctcgg
     3961 cataccctct cctacgacga tggaatatat cgatgactat ttcgcgaatt acctgatgga
     4021 ccccggcctg gcggagaacg agacgtacaa gtacgcaagc agaattcagc atccgatgcc
     4081 gaagggaggg acgcaactgg accgcgccat agcgatgctg aaagagactc cgcttacaaa
     4141 ccaggctata gtggagatcg gatctccgga cgaccacgat gtttgcatcg gcaacgatgg
     4201 aaaactcgac cctccctgcc tgagactgtt ggatttcaag gcagtccccg caggcgatga
     4261 gttggtgctc tctttgagct gttacttccg ttcctgggac ctgtgggccg gatttcccac
     4321 gaatctcggc ggcctagaac tcctgaagag atatgtggcc tctgaagcag gcttaaaaaa
     4381 cggaccgata tatgcttata gcgcgggcgc tcatatttac gggtatcagg aagaaatcgc
     4441 ccgaataagg acgttgcggc aaaagtaatc aataagcgca tgaagacgac tttttatgaa
     4501 tgacatttat aagttaatta aaaaaaatca gcgacaactt tttcattttt cttgactcgc
     4561 aagtaaattg ccttatataa tagtaattct tcatcatttc atctcatttt ggccggccga
     4621 tcgaagaggt cagaagaagc gggagcaaag aaataaagga gccagggggc caggggccag
     4681 tggcgagggg aagcaatgca gatcctgacc cccggacccc ggtatctgga ccccgatgtc
     4741 agccatgggt tttacaattg cagtcgccgg caagggtggc acgggcaaga ccagcctgtg
     4801 cggccttacc attcgctatt tgacagaaaa gaagaagggc gccgttctcg ctgttgatgc
     4861 tgacgcgaat gcgaacctga acgatgtgtt gggcgtcagg atccacgcca cggtcgggaa
     4921 gctgcgggag tcctccctcg aagcgatcaa gtcgacggcc ccgcgtccgg gcgggatgag
     4981 catggagcag ctcttcgact accagatcca gcaggcgctg gcggagtcca agggtttcga
     5041 cctcctgacg atgggccggc ccgaaggcgc gggctgttac tgcgcggcga acaacatcat
     5101 tcgcaagtac atggacaaac tggccgactc gtacccctat acggtcatgg acaacgaggc
     5161 cggcctggag catctgtcga gacgcacgac gcaggatacg gacctcctca tgattatcag
     5221 tgattcgtca gtgcgcgggg ttatgaccgc cggccggatc ggcgaactgg taaaagaact
     5281 gaacctgaac gtcaagcgct ctgtccttat cgtaaatcgg gtgcaggacg gaacgagcga
     5341 cgatccctcg ctcctgcagg ttatcaggca gcaga
//
LOCUS       DASSLD010000118         3601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1035597, whole genome shotgun sequence.
ACCESSION   DASSLD010000118 DASSLD010000000
VERSION     DASSLD010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035597"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /gene="gltX"
                     /locus_tag="VFK23_02245"
     CDS             complement(<1..1113)
                     /gene="gltX"
                     /locus_tag="VFK23_02245"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET7317935.1"
                     /translation="MSTQTPVRVRFAPSPTGALHIGGVRTALFNWLFARHHGGKFILR
                     IEDTDQARSTDESIRIIIDGMKWLGMDWDEGPTVLPDGTVKGFRQTERMAIYREYADR
                     LLREGKAYYCYCSPEELEARRKAAMAAGKPPKYDRTCYGRREPVPGRTPVIRFHSADE
                     GETVVRDLIRGAVTFENKQLDDLIIMRSDGFPTYNFAVVVDDVTMKISHVIRGDDHLN
                     NTPRQIQIYQALGFYAPAFAHLPMILGPDKTKLSKRHGATAVTEYVDLGYLPEALVNY
                     VARLGWSHGDQEIFSVQELIEKFTLDSVGKAPSVFNQEKCLWLNHHYIQQADPGRLAA
                     LVLDLLRKDNIVSTGSGPDREWMKKLVKILTERSHTL"
     gene            1431..1877
                     /locus_tag="VFK23_02250"
     CDS             1431..1877
                     /locus_tag="VFK23_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317936.1"
                     /translation="MDIRVFTTKPVVEIEGVKYHHHANGGGMVAETAEVADTVYVGPF
                     AKICGKCRLKDEAFVFGNAWVFDKAEISGRAEIFGNAKVFGNAKISGDAKVFGFSSVY
                     GNAVVTGAAEVRDKAEIGGNTRLSGNARVGGEAKILGDVGVEKKAA"
     gene            complement(2081..2422)
                     /gene="atpE"
                     /locus_tag="VFK23_02255"
     CDS             complement(2081..2422)
                     /gene="atpE"
                     /locus_tag="VFK23_02255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HET7317937.1"
                     /translation="MKKFSVIMILVVGLMLLATAAFAEEAATHDAGKFAFFGTVAAAS
                     AIGIGIAALGTGIGMGLGIGRAVEGIARNPEASGKIMTTMIVGLALIESLAIYTLVVV
                     LILLYAKPFGV"
     gene            complement(2624..3322)
                     /gene="atpB"
                     /locus_tag="VFK23_02260"
     CDS             complement(2624..3322)
                     /gene="atpB"
                     /locus_tag="VFK23_02260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET7317938.1"
                     /translation="MMKEAPVLLHFNIPGLSEEAQIVVTYSWLAMAILIGLALVARFS
                     LKKTAPTGVQNLLETVVGGLENFVVDIMGTEGTHYLSLIGTLFLFILVCNLQGLIPGF
                     DSPTANINTTLALALVSFAATHYIGVKRHGIGYIKHFMGPMWGLAPLMFPIELISHFA
                     RVMSLTFRLFGNMVAKHKLLLVLALLAPYIAPVPILGLGLLVSFVQAGVFTLLTMLYL
                     YGSIEAAHLGGEHH"
BASE COUNT          789 a          944 c         1093 g          775 t
ORIGIN      
        1 gagcgtgtgg cttcgttccg taaggatctt caccagcttt ttcatccatt cgcggtcggg
       61 cccgctgccc gtggaaacga tgttgtcttt gcgcaggaga tcgaggacca aggccgcgag
      121 ccgcccggga tcggcctgct ggatatagtg atggttcagc cagaggcact tttcctggtt
      181 gaagaccgat ggagccttgc cgacactgtc gagcgtgaac ttttcgatga gctcctggac
      241 cgagaaaatt tcctggtccc cgtgcgacca gccgagccgg gcgacgtagt tcacgagtgc
      301 ttcaggaaga tagccgagat ccacgtactc cgtgaccgca gtcgcgccgt ggcgcttcga
      361 cagtttcgtc ttgtccgggc ccaggatcat agggagatgg gcaaaggccg gggcatagaa
      421 gcccagggcc tggtagatct ggatctggcg cggggtgttg ttcagatggt cgtcaccgcg
      481 gatcacgtgc gagatcttca tggtgacatc gtccacgacc acggcaaagt tgtaggtcgg
      541 gaatccatcg gagcgcataa tgatcaggtc gtcgagctgc ttgttttcga acgtgaccgc
      601 accccggatc aggtcgcgca cgaccgtctc gccctcgtcc gcggagtgga agcggatgac
      661 aggcgttctt cccgggacgg gctctcttcg gccgtaacaa gtgcggtcgt atttgggcgg
      721 cttgcccgcc gccatggcgg ccttccgccg cgcttcaagc tcctcgggcg agcagtagca
      781 gtagtaggcc ttgccctccc gcagaagccg gtcagcgtac tcacggtaga tggccatgcg
      841 ttcggtctgg cggaagccct ttacagtgcc gtccggcagg acagtcggac cctcgtccca
      901 gtccatcccg agccatttca tgccgtcgat gatgattctg atggactcgt ccgtggatcg
      961 cgcctggtcc gtgtcttcga tcctgagaat gaacttcccg ccgtggtggc gcgcgaaaag
     1021 ccagttgaac agggccgtgc gtacgccccc gatgtggagg gcgcccgtgg ggcttggtgc
     1081 gaaacggact ctaaccggtg tttgggtgct catttatgct ctgggctcct tgtacatgcg
     1141 tcaatttagc gctgtaagag gccattatag caaattatcg gatgaatcaa aagtaaattt
     1201 gatgttctta ctctttgcac cttcgtggtt tcacgggctg aaggagaaca ataggatgcg
     1261 cattgtatca cttgcagttg tttttctcga aaaaaagggc ttgtttttga caaaacggaa
     1321 tgccttggat atgatttaaa taattaataa ttgaaatttg aagtgcgcct ctggaagggg
     1381 cagaggcgcg cgcgtgcata tcgtcgcatt tttcaagtcg gaggtgcatt atggatatca
     1441 gggtcttcac gacaaagccg gttgtagaga ttgagggcgt caaatatcat catcacgcca
     1501 atggcggcgg catggtggcc gagaccgcgg aagtggcgga taccgtgtat gtcggtccct
     1561 ttgccaagat ctgcggcaag tgccggctga aggacgaggc ctttgtcttc gggaatgctt
     1621 gggtcttcga caaggccgag atttcgggcc gggcggaaat cttcgggaac gccaaggtct
     1681 tcggcaacgc gaagatctcg ggcgacgcaa aagtcttcgg attttccagc gtgtacggta
     1741 atgccgtggt gacaggcgct gccgaggtgc gcgacaaggc cgaaatcggc ggaaacacga
     1801 ggctctcggg caatgcacgg gtcggcggag aggccaagat cctgggcgac gtaggcgtgg
     1861 aaaagaaagc cgcgtgagaa atttcactag ccgtcatccc cgaagtcctc tatcggggag
     1921 ccggtttaat cgtgatagag ataagaacca tattcccggt aaattcattc gggaatgacg
     1981 tttttttgag gtctcctgcg cagcttcgtt gcgggaaatt aaaaaaggga cggttcattg
     2041 aaccgtccct ttttgttatt ccactggccc ccccaggtgg ttacacgccg aagggttttg
     2101 catagagcag gatgagcaca acgaccagcg tgtagatggc cagagactcg atcagggcca
     2161 gacccacgat catggtggtc atgatcttgc ccgatgcctc ggggttgcgg gcgatacctt
     2221 cgaccgccct gccgatgccc aggcccatgc cgatgccggt gccgagggcg gcgatgccga
     2281 tgccgatcgc ggaagcagct gccacggtgc cgaagaatgc gaacttgccc gcatcatggg
     2341 tcgccgcttc ctccgcgaac gccgccgtcg ccaagagcat caagccgacg acgaggatca
     2401 tgattactga gaactttttc atggattcgc ctcctttcca aaaattttaa atagatcttt
     2461 accggatatc accggggcgc taccgcccgg gcgcatcctc catgggagga acatcaagcc
     2521 ccggggtgat gtgaatgtcg gattgtctat tgtcgattaa tagacacttg ttagacgtct
     2581 cttcttccaa atcgacgatc atcaatcatc gatcaacaat cgcctagtgg tgttctccgc
     2641 cgagatgagc ggcctcgatg gagccgtaca gatagagcat cgtcaaaagc gtaaacacgc
     2701 ctgcctgcac gaaggaaacg aggaggccca gtcccagaat cgggaccggc gcgatatagg
     2761 gcgcgagcag cgcaagcacg agcaggagct tatgcttcgc caccatgttg ccgaagagac
     2821 ggaaggtgag ggacatgacg cgcgcaaagt gactgatcag ttcgatgggg aacatgagcg
     2881 gcgcgagtcc ccacatgggc cccatgaaat gcttgatgta gccgatgccg tgccgtttta
     2941 cgccgatgta atgggtcgcg gcgaacgaaa cgagggcgag ggcgagggtc gtattgatgt
     3001 tcgccgtggg tgaatcaaaa ccggggatga gcccctggag gttgcatacg agaatgaaca
     3061 ggaagagcgt tccgatgagc gacaggtagt gcgtgccctc cgttcccatg atgtccacca
     3121 cgaagttttc gagccccccg acgacggtct ccagaaggtt ctgcacgccg gtgggagcgg
     3181 tcttcttgag cgagaagcgc gccaccagcg cgagcccgat caggatggcc atggcaagcc
     3241 aggaataggt cactacgatc tgtgcctctt ctgaaagacc ggggatgttg aaatggagga
     3301 gaaccggcgc ttctttcatc atacgtacgc tccttgaaaa gacggtcctg atacaggcaa
     3361 aaatctacag gaacccctat tttttgtcaa gaaaaaaccc ggtaactcct cataaaattt
     3421 cttaataatc gcatatagga ttcttaatga aacgggatcg agatcgaatc agggctcggg
     3481 caggggggaa gtgacgccgc ttaaagcttc ggctcaaggc tgtttcgctg gtacccggca
     3541 tgccgccgat cgcgatgagg ttaccctcgc tcgttactac gccggtgagc agagcaaacc
     3601 t
//
LOCUS       DASSLD010000119         2521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_673424,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000119 DASSLD010000000
VERSION     DASSLD010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673424"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFK23_02265"
     CDS             complement(<1..543)
                     /locus_tag="VFK23_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7317939.1"
                     /translation="MKKPHAPLIIMILFGWLASAGAAEKITTLDEAYRAALSSNETIK
                     IAEEDVSQSNYRMDQAWSFLYPKLNARASYTRFNKTLPPEGGDFLFQPLDQYQAALVL
                     TQPLYTGGRTLAAYRAAKKLGETSTNSLGLTRQDLMLRVAEAYYGVLRAQKSVEISKR
                     SLERIEQHQQITKREAGTRRN"
     gene            complement(581..1180)
                     /locus_tag="VFK23_02270"
     CDS             complement(581..1180)
                     /locus_tag="VFK23_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7317940.1"
                     /translation="MDGDTRAAILAAARRVFAERGLDGTSVREVAEAANVNNAMIYYH
                     FKDKDDLYQSVLSDSFSALKAIWDNEIFKSQAPVRQKIQQYIEGAIRFHQNNEDLRRI
                     MAMEFAASGGTGGLVCEKYFADSYNRLVGIIKDGMRTGELKKFDPAHAVASLIGVIIH
                     NFILQPMAEHVNGKSVNLSSRKFGAFVTELFFNGLGSNT"
     gene            complement(1404..2198)
                     /locus_tag="VFK23_02275"
     CDS             complement(1404..2198)
                     /locus_tag="VFK23_02275"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HET7317941.1"
                     /translation="MRHSLHFVVWCIFIFTAAALAACGGKSSSAPETAPVAKVTRDAF
                     LTGSQVISIPPVSSRATGTFSLKLDSVTGQLSGSLKLSQMAHKVTAVGIREGAIGANG
                     DLIVSLVETPAGSGVWTLPADATALTTTQTDLFRNAGLYADVRTDVFPNGEIRGQFMS
                     FAANIQPIFDAQCVSCHDPGKQAQFTYLFLAAPYSFYSLVNQPATQSSGIRVIPYDAA
                     GSFLLSKTTGNGLPAGVSRMPPGGPFLSDTEENLIRTWIIMGALND"
     gene            complement(2230..>2521)
                     /locus_tag="VFK23_02280"
     CDS             complement(2230..>2521)
                     /locus_tag="VFK23_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317942.1"
                     /translation="QFCVDCHNGNGQAANTGLFLDTGDSYRLLVNQPATSPTATGIRV
                     TPLDPTHSVLYERISGTGLPAGAAQMPLLKPPLSAHDQNLIKSWIDMGARND"
BASE COUNT          484 a          706 c          745 g          586 t
ORIGIN      
        1 gtttctccgg gtccccgcct cgcgcttggt gatctgctgg tgctgttcaa tccgctccag
       61 cgatcgtttg cttatctcga ccgacttctg cgccctgagg acgccgtaat acgcctcggc
      121 gaccctgagc atgaggtcct gcctggtaag ccccaggctg ttggtgctcg tctcaccgag
      181 tttctttgcg gcgcggtagg ccgccagtgt ccggccgccg gtgtagagcg gctgcgtcag
      241 aaccagtgcc gcctgatact ggtccagggg ctgaaagagg aagtctcctc cttcgggcgg
      301 cagcgttttg ttgaagcgcg tataggacgc cctcgcgttc agtttcggat acaaaaagct
      361 ccaggcctgg tccatgcggt agtttgactg ggaaacatct tcttcggcaa tcttgatggt
      421 ttcgttgctg ctaagagccg cgcggtacgc ttcgtcaagc gtcgtgatct tttccgccgc
      481 tcccgcggaa gccagccaac cgaaaaggat catgataatg agtggggcat gcggtttttt
      541 cataggtgcg acccttaata cgaaataacg gtacggcagt ttatgtattc gatcccaggc
      601 cgttaaagaa aagttccgtc acaaaggcgc cgaacttcct gctggacaaa ttcacgcttt
      661 tcccgttcac gtgttcagcc atgggctgca ggatgaaatt gtggatgatg acgcctatca
      721 gcgacgccac ggcgtgagcg ggatcgaact tcttcagttc acccgtccgc atgccatcct
      781 tgataattcc aacaaggcga ttatagctgt ccgcgaaata tttttcacag accaatccgc
      841 cggtgccccc actcgcggcg aactccatgg ccatgatccg tcgcaggtct tcgttgttct
      901 gatgaaagcg gatggcccct tctatgtatt gttgaatctt ttggcggacc ggggcctggg
      961 acttgaatat ctcattgtcc cagattgcct tgagcgctga gaaggaatcg gagaggaccg
     1021 actggtaaag gtcgtccttg tccttgaagt ggtagtagat catcgcgtta ttgacgttgg
     1081 ccgcttcggc gacctcgcgg accgaggtgc cgtcgaggcc ccgctcggcg aacacacgcc
     1141 gcgccgccgc gaggatcgcg gccctcgtat cgccgtccat gcgccccgct ttcggccggc
     1201 gacccgccgt ccgtctctgg cttttcgatg atcggttcat agtaattttt tatttaacta
     1261 atcggttaat taattagaga caaacatacc tgcttccggt tgatttgtca agaccaattt
     1321 tgccggtggc gggtgcggtg ttttgcagat aaggacggga cccagcgggt atcgggggcc
     1381 catttcggat accgtgaggc gggtcagtcg ttcaacgccc ccatgatgat ccaggtcctg
     1441 atgaggtttt cctcggtgtc ggacaaaaac ggtccgccgg gaggcatccg gctcaccccc
     1501 gcgggcaagc cgtttccggt cgttttgctc aataaaaagc tgccggctgc gtcataaggt
     1561 atcacccgga tgccgcttga ttgcgttgca ggctgattca cgagcgaata aaaagaatag
     1621 ggcgccgcca aaaagaggta ggtaaactgg gcctgtttgc cgggatcgtg gcaggacacg
     1681 cattgcgcgt cgaatatcgg ctggatgttg gccgcgaagg acatgaactg accgcggatt
     1741 tctccgttcg ggaagacgtc cgtacgaaca tcggcgtaga gcccggcatt cctgaacagg
     1801 tccgtctgcg tcgttgttaa tgccgtggcg tcggccggca gggtccatac gccgctgccg
     1861 gcgggcgttt caacgaggga gacgatcagg tcaccgtttg ctcctatggc accctcccgg
     1921 attccgactg cggtaacctt atgcgccatt tgggacagct tcagcgaacc ggacaactgg
     1981 ccggtgacac tgtccaattt taggctgaag gtgccggttg cgcgtgatga taccggaggt
     2041 atcgatataa cctgacttcc tgtcaggaag gcgtcgcgcg ttaccttcgc gaccggggcg
     2101 gtctccggtg cactgctgct cttgccgccg cacgccgcga gagccgccgc tgtaaagatg
     2161 aagatacacc acaccacaaa atggagcgaa tgacgcatgg aatttctcct gttcctcgaa
     2221 tctgcgcaac taatcgttcc gggcccccat gtcgatccag ctcttgatca ggttctgatc
     2281 gtgcgcggag agcggaggtt tgagcaaagg catctgggcc gcgcccgcag gaagcccggt
     2341 tcccgatatt ctttcgtata agacgctgtg tgttggatcg aggggcgtca ccctgatgcc
     2401 ggtggccgtc ggcgatgtgg cgggctgatt caccagcaga cggtatgagt ctcccgtatc
     2461 caggaagagc ccggtatttg ccgcctggcc gttgccgttg tgacaatcga cgcagaactg
     2521 a
//
LOCUS       DASSLD010000120         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_92020,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000120 DASSLD010000000
VERSION     DASSLD010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92020"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="VFK23_02285"
     CDS             <1..1224
                     /locus_tag="VFK23_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7317943.1"
                     /translation="VLYQVFINILSFYVVALLSGYLSDRLLKTRQELHAKSMDYEDLR
                     TMQDHILKSVRSGILTMDLEGKITSCNPAAEYITGYTYAELKIRWQDLFGSSIKGLFG
                     HTNDLMERPFRFDGPIVKKNGDTAILGTSVSLLKDDKNTVHGIILIFQDITKLVEMEE
                     KVRRQEQLAVVGSLAAGIAHEIRNPLASLSGSIQVLQGELDLKGDDKHLMDIVVRETD
                     RLNTIISEFLEYARPQTAHAEKILLLPVLDETVMLLKNSHDFREGITIRAQVDPQVVI
                     NGDAQRMRQVFWNLLINACQAMPDGGEITVSAMPISRSDDDSGWCEIIIKDTGHGIAH
                     EYLDKIFDPFFTTKTGGTGLGLAIVERIIEDHGGTIEVESETGRGTKFRIRLPVLEEA
                     ASLLTRSGSIMDQLG"
     gene            1239..>2417
                     /locus_tag="VFK23_02290"
     CDS             1239..>2417
                     /locus_tag="VFK23_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7317944.1"
                     /translation="MSRILVVDDEQSMRDFLSIMLKKEGYDVVTAENGGGALIAIQAE
                     IFDLVITDVRMAGVDGIDVLKTVKEVSPETVVIMITAFATAETAVEAMKLGAYDYITK
                     PFKVDEIKLVIQKALEKRYLRKENILLRREIESRAGFENFIGTSAPMQRVFSLIRQVA
                     DTKSTVLITGESGTGKELVAKAIHFNSSRKGGPFVTVNCGALPETLLESELFGYMKGA
                     FTGATSNKQGLFEAATGGTIFLDEISATTPALQIKLLRVLQEREFMRVGGTVDIKMDA
                     RVIAASNRDLLSEVSKGTFREDLYYRLNVIPIPLPSLRERREDIPLLVEFFLKKFSGG
                     KEPKKITPEAVNLLMNQRWPGNVRELENTIERMVIMTANHTVSLENIPENIKSVQSSA
                     E"
BASE COUNT          646 a          576 c          675 g          520 t
ORIGIN      
        1 gtgctgtatc aggttttcat caatatcctt tctttttatg tcgtcgccct cctgagcggt
       61 tatctgtccg atcggctgct taaaacccgc caggaacttc acgcaaaaag catggattat
      121 gaggacctga ggaccatgca agatcacatc ttaaaaagtg tgaggagcgg gatcctcacc
      181 atggaccttg aggggaaaat aacatcctgc aaccctgcag ctgaatatat taccggatac
      241 acctatgcgg agctcaagat ccgctggcag gatctcttcg ggagtagcat caagggactg
      301 tttggccata cgaacgacct catggagcgc ccgttccgct ttgacggacc gatagttaag
      361 aaaaatgggg atacggccat actcggaacg tccgtttctc tgctcaagga cgataagaac
      421 accgttcatg gtatcattct tattttccag gatatcacca aactggtcga gatggaggaa
      481 aaggtcagga gacaggaaca gctcgccgtg gtcggcagcc tggcggccgg catagcacat
      541 gaaatacgga accctctcgc gtcgttgagc gggtcgattc aggtgttgca gggtgaactt
      601 gacctcaaag gagatgacaa gcacctcatg gatatcgtgg ttcgtgaaac ggaccgccta
      661 aacacaatca tctcggaatt tttggaatat gcacgaccgc aaaccgctca cgcagaaaag
      721 atcctgctct tgcccgttct tgatgagacc gtcatgctcc tcaaaaacag ccatgatttc
      781 agggagggga tcacgatcag agcgcaggtt gacccgcagg tagtcataaa cggtgatgcc
      841 caaaggatgc gtcaagtctt ctggaacctc ttaattaatg catgccaggc gatgccggac
      901 ggaggcgaga taacggtgag cgcgatgcca atctcacgca gtgacgacga ttctggatgg
      961 tgtgagatta tcataaagga taccgggcac gggatcgccc atgaatatct tgataaaata
     1021 ttcgatccgt tttttacaac aaagacgggc ggcacaggcc tcggactggc gattgtggag
     1081 cggattattg aagatcacgg aggcaccatt gaagttgaaa gcgagaccgg cagaggaacg
     1141 aaattccgta tcagacttcc cgtgctggaa gaggctgcct ctctgttgac caggagcggc
     1201 agtatcatgg accaacttgg ttaatggtat gcgagaaaat gagcagaata cttgtcgttg
     1261 acgatgagca gagcatgcgg gatttcctct cgatcatgct gaaaaaagaa ggctatgatg
     1321 tggttacggc cgaaaatggc ggtggtgcgc tcatagcgat ccaggccgag atcttcgacc
     1381 ttgtgatcac cgatgttagg atggctggcg ttgacggtat agatgtcctg aagacggtca
     1441 aggaggtatc tccggagacc gtggtgatca tgatcaccgc atttgcgacc gcagagaccg
     1501 ctgtcgaggc catgaagctc ggggcctatg attatatcac gaaacccttc aaagtcgatg
     1561 aaatcaagct ggtgatccag aaggcgctcg aaaaacgcta tctcagaaaa gaaaatattc
     1621 ttctccgaag agagatcgag tcccgcgcag gtttcgaaaa ctttatcgga acgagtgctc
     1681 ccatgcagag ggtgttctct ctcatacggc aggtggcgga taccaaaagc accgtgctca
     1741 taacggggga gagtggaacg ggcaaagagc ttgtggcgaa ggccattcac ttcaacagtt
     1801 cgcgaaaggg aggccccttc gtgaccgtaa attgcggagc cctgcccgag accctgcttg
     1861 agagcgagct ctttggttac atgaagggag cgtttaccgg cgcgacctcg aacaagcagg
     1921 ggctgtttga agcagccacc ggagggacta ttttcctcga tgaaatcagt gccacgacac
     1981 ctgcgttgca aatcaagctg ctgagagtgc tgcaggaacg cgagttcatg agggtcggcg
     2041 gaaccgtcga tatcaagatg gatgcgcggg taattgcggc gagtaacagg gatctactgt
     2101 ccgaggtctc aaaaggcaca ttccgcgaag acctctacta ccgtcttaac gtcatcccga
     2161 ttcctctccc ctcgcttcgc gaaagaaggg aagatatccc gctcctcgtc gaatttttcc
     2221 tgaagaaatt ttcaggtggg aaggagccaa agaagatcac cccggaggcg gtgaacctgc
     2281 tcatgaatca gcggtggccg ggaaatgtgc gggagctgga aaataccatt gaacgcatgg
     2341 taatcatgac ggccaatcac acggttagtc tcgaaaacat tccggaaaat ataaaaagcg
     2401 tacagtcaag tgcggag
//
LOCUS       DASSLD010000121         3253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_53023,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000121 DASSLD010000000
VERSION     DASSLD010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53023"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VFK23_02295"
     CDS             complement(<1..1002)
                     /locus_tag="VFK23_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7317945.1"
                     /translation="MQEARILIMDDEERERRRLEQYLGGKGYDVLAVGTVQDAVQAIR
                     RERFDVFLTDCNIPGVDALRTSDEARKINPDMAVVIMTAFGTIETAVRAIKAGAYDYL
                     AKPVDPEQLVVLIERISERRDLIRENTELKERLREKYKFDEIVSISHAMEEVLNLAGR
                     VAASNATVLLRGESGTGKELVAKAIHYHSPRAAFPLIKVNCAALPETLLESELFGHEK
                     GAFTGATARRIGRFEAADKGTIFLDEIGELAPGMQVKLLRVLQERAFERIGGNQTIKT
                     DVRVIAATNRNIEKALADGTFREDLYYRLNVVTIVIPPLRERKEDIPGLLDFFIKKYR
                     "
     gene            complement(1016..1963)
                     /locus_tag="VFK23_02300"
                     /pseudo
     CDS             complement(1016..1963)
                     /locus_tag="VFK23_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
     gene            complement(1960..2412)
                     /locus_tag="VFK23_02305"
     CDS             complement(1960..2412)
                     /locus_tag="VFK23_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7317946.1"
                     /translation="MTNLELLKKTMLFRGSTREDLEMVLGLFQEREIRPNANIFTEKM
                     PGEALFIVKSGAVKITMMAGEGEEMGLLALGPGDFFGELALIHEDTRLVNARAETAVD
                     LLLLTRKDFYALLELDPRTASRLLIAVSKLLAMRIQAYSDKLKELLLS"
     gene            complement(2523..>3253)
                     /gene="coaBC"
                     /locus_tag="VFK23_02310"
     CDS             complement(2523..>3253)
                     /gene="coaBC"
                     /locus_tag="VFK23_02310"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HET7317947.1"
                     /translation="VRGQGRLADTDRIIGELSAMFLSKDLSGKTVLVTAGPTRESIDA
                     VRYISNASCGKMGYALAAAARERGADVVLISGPTHLAVPLGVRAVPVVTAEEMRVAVL
                     EQYDHSDIVIMAAAVSDFKPARPSARKIKKEEASGVLQLERTVDILKALGDSGGKQLL
                     VGFAAETDDVEHNALKKMKDKNLDMIVVNDLLRDGSGFGTDTNAVTIFDRSGRRQELP
                     TMPKTDVARRIISAVVDLMNEEVP"
BASE COUNT          585 a         1014 c          952 g          702 t
ORIGIN      
        1 gcggtatttc ttgatgaaaa agtccaaaag gccggggatg tcttccttgc gttcgcgcag
       61 gggcgggatc acgatggtga ccacgttcag gcggtaatac aggtcttccc ggaacgtccc
      121 gtcggcgagc gccttttcaa tgttccggtt cgtcgccgcg atgacgcgaa catcggtctt
      181 gatggtctgg ttgccgccga tgcgctcgaa ggcacgctcc tggagaacac gcaggagctt
      241 tacctgcatg ccgggcgcca gttcgccgat ctcgtcgaga aatatcgtcc ccttgtccgc
      301 agcctcgaac ctcccgatcc gcctcgccgt cgccccggta aaggcccctt tttcatggcc
      361 gaacagttcg ctctccagga gcgtttcggg taaggccgcg cagttaacct tgatgagcgg
      421 aaaggcggca cgggggctgt gataatggat ggctttcgca accagttcct tgccggttcc
      481 actctcgccg cggagaagca ccgtagcgtt gctcgcagcg accctccccg ccaggttcag
      541 gacttcttcc atggcatggc tgatgctcac gatttcgtcg aacttgtact tctcccgcag
      601 gcgctccttg agttccgtat tctcgcggat caggtcgcgg cgctcggaga tccgttcgat
      661 gagcacgaca agctgttccg ggtcaacggg cttggcgagg taatcgtagg cgcccgcctt
      721 gatggcccgc accgccgttt ctatggtccc gaaggcggtc atgatgacta ccgccatgtc
      781 cgggttgatc ttgcgggcct cgtccgacgt cctgagcgcg tccacaccag ggatattgca
      841 atcggtgaga aagacgtcga aacgctcccg ccttatggcc tgtacggcgt cctgcacggt
      901 cccgaccgca agcacatcgt accccttccc gccgaggtac tgctccagtc gccggcgctc
      961 ccgctcctcg tcgtccatga tgagaatcct ggcttcctgc atgctgttcc ctcagtcaaa
     1021 tgtgcttgtt caatctctca tccagatcca gcacgatccc ttcccgcagg ccccaaacgc
     1081 tcacgagcat cgacatgcgg ccgaaccgcg ccatgacttc ccgggttatg acggccccgg
     1141 caatgatgat gtcttcccgt cccggctcaa gcccccggac gttctttcgt tcttcacgcg
     1201 tcagtctgct caacgcgaga atcatgtcgt cgatgccgtt gcgcgtcagc gtggacccgt
     1261 tgatcctggc gggatcgtac gcttcaagcc ccaggtttat cgaggccagc gtcgcgacgg
     1321 tacccgcggt gccgatgaaa agacccggcg ttacgctttg aagtacgccc aagcggctgt
     1381 ccagttcgtc cctgaccatg cctcacatcc gctcgagatc gttgccggaa ggcagatcgt
     1441 gcgtgaggaa ccgttcggtg aggtacacgg ctcccagcgg caggctggcg acgacggagt
     1501 ccccgtctcc gcgagcgacg atgacttcgg tgcttccccc tccgacatct atgacgcagg
     1561 aggcagccgg tgatgggctg cggccggcgt ccttcaggga gtgcctcacc cccaacaggg
     1621 taaggcgcgc ctcctcttca ccggaaacaa cctggatatc gattccggta tgtcctttga
     1681 cggtggagag aaattggcgg gtgttcgacg cattccggag ggcgctggtt ccgatcgcgg
     1741 aagtgtgctg gacgccgtgc atgcgaatgg cttccgcgaa atcaagaaga acgttcaccg
     1801 accgctccgc cgccccggcg tcgatcatac ccgagcgttc cagtccctgt cccaggcggg
     1861 tgattctccg gtcggagaaa acttcccgga gcgagccgcg gtcggtttcg gcgatgagaa
     1921 ggcggagcgt attggttccg atatcgatcc cggcaaggat cacgacagga ggagttcctt
     1981 gagtttatcg ctgtaggcct gaatgcgcat cgccagcagc ttcgaaaccg ctatgagaag
     2041 acgggaggcg gtccggggat ccagttcgag caaggcgtaa aaatccttgc gggtaagcag
     2101 caggagatcg acggccgttt cggcccgcgc gttaaccagc cttgtgtcct cgtgaatgag
     2161 cgcaagctcg ccgaaaaaat ctcccgggcc gagcgcgaga agtcccattt cctctccctc
     2221 cccggccatc atggtgatct tgacggcgcc gctcttcacg atgaagagcg cctcccccgg
     2281 catcttttcc gtaaagatat tggcattggg cctgatctcc cgctcctgga aaagccccag
     2341 gaccatttcc aggtcctccc tggtgctgcc cctgaagagc atggtttttt taaggagttc
     2401 aagatttgtc atgggtgtcc gccggcacgg acaggaaaac tttgcgcgat cattataggt
     2461 tggcttgttc tttttgtcaa gaatcaatga gaatgcggta ccggaaggca ggatgccgga
     2521 gcctacggaa cctcttcgtt catgagatcc acgactgcac tgatgatgcg tctggcgaca
     2581 tcggtcttcg gcatggtcgg cagctcctgc cttcttccgg aacggtcgaa gatcgtcact
     2641 gcgttcgtgt cggtcccgaa gcctgacccg tcccggagaa ggtcgttgac gacgatcata
     2701 tccaggtttt tatccttcat cttcttcaac gcgttatgct cgacatcgtc cgtttccgcg
     2761 gcaaagccta ccaggagttg tttcccgccg ctgtcgccca gcgccttgag aatgtcgacg
     2821 gtccgttcga gctgcagaac gccggaggcc tcttccttct tgatctttct cgcggacgga
     2881 cgggctggtt tgaaatcgga aacggccgct gccatgatga cgatgtcgct gtggtcatac
     2941 tgttcgagga ccgcgacgcg catctcctcg gcagtgacga cgggaacggc cctgacgccc
     3001 aaagggacag cgagatgggt cggccctgaa atgagcacca cgtcggcccc ccgttcacgc
     3061 gcggccgcgg ccagggcgta tcccattttc ccgcatgaag cgttgctgat gtacctgacg
     3121 gcatcgatgg attcccgggt cgggcctgct gtcacgagca ccgtcttgcc ggaaaggtct
     3181 ttcgacagga acatcgcgga cagctctccg atgatccggt cggtgtctgc gagtctgccc
     3241 tgccccctca cgc
//
LOCUS       DASSLD010000122         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1129031, whole genome shotgun sequence.
ACCESSION   DASSLD010000122 DASSLD010000000
VERSION     DASSLD010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129031"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VFK23_02315"
     CDS             <1..982
                     /locus_tag="VFK23_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006035153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7317948.1"
                     /translation="NLFYFKLLSYTPNYEHRTPNFLLRTMVNELAIKVDVDTDRGTRV
                     GVPNLLSLFDDFGIKATFLFSLGPDNTGRAIKRIFRPGFLGKVSRTSVVSTYGIRTLL
                     NGVLLTGPHVGRRNEGVMRRAREKGHEVGIHCYDHIRWQDGLGKMSREDVTKEFGRAR
                     REYERIFGEPAKTAGSAGWQANAYSLAAYDDAGLLYGSDSRGTHPFFPRVNGIVFRTI
                     QIPTTLPTLDELLGRPEYPDEKIVNHYLSLMKPGALNVLTVHAEIEGMRYRALFRELL
                     ERIKSKPVQVVRLDDHVHGLLKNPAVIPVCDLAAGTVDGRSGMLAVQSCR"
     gene            1202..>2016
                     /gene="mtnA"
                     /locus_tag="VFK23_02320"
     CDS             1202..>2016
                     /gene="mtnA"
                     /locus_tag="VFK23_02320"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HET7317949.1"
                     /translation="MMIPTVEWKDGIVRMLDQTKLPLETVYTDCRDYQTVARGIKELW
                     VRGAPAIGVSAAMGLALGARQIAAGSFEEFWPRFEEICGHMAATRPTAVNLFWAIERI
                     KAFVQSNKDKSLDEIRTMVVEESQRMLEEDIATNKKMGAYGAAFIADGDNIITHCNAG
                     SIATAGYGTAEGVMRAAREAGKNIHVYVDETRPMLQGARLTAWELMQEGIPCTLITDN
                     MAGYFMYRDMIDLAIVGADRVARNGDVANKIGTYGLAVLCKEHGIPFYVAAPVS"
BASE COUNT          439 a          597 c          597 g          383 t
ORIGIN      
        1 aaaccttttt tacttcaagc ttttgtcata tactccgaac tacgaacacc gaactcccaa
       61 cttcttgctc cgaactatgg ttaatgaact cgccatcaaa gtggacgtcg ataccgaccg
      121 gggcacgcgc gtcggcgtgc ccaacctcct ttccctcttc gatgatttcg gcatcaaggc
      181 cacgttcctc ttctccctcg gtcctgacaa tacaggccgg gcgatcaagc gcatctttcg
      241 tccggggttc ctcggcaagg tgagccggac aagcgtcgtc tcgacctacg gcatccggac
      301 actcctgaac ggcgtcctcc tgacgggccc gcacgtgggc cgcaggaacg aaggggtgat
      361 gaggcgtgcg cgggaaaagg ggcatgaggt cggaatccac tgctacgacc acatccgctg
      421 gcaggacggc ctcgggaaga tgagccggga ggatgtgacc aaggagttcg gcagggcgag
      481 gcgggagtac gaacggatat tcggagagcc ggcgaaaacc gccggttcag cgggctggca
      541 ggccaatgcc tatagccttg cggcgtatga tgacgcaggt ctgctttacg gaagcgacag
      601 ccggggtact cacccgttct tcccccgggt gaatggcatt gtattcagga ccatccagat
      661 acccacgacg ctccccacgc tggacgagct cctgggacgg cctgagtatc ccgacgaaaa
      721 aatagtaaac cattatctgt cacttatgaa gccgggcgcc ttgaacgtgc tcacggtcca
      781 cgccgagatc gagggcatgc gctatcgtgc actcttcaga gagcttctcg aacggatcaa
      841 gtcgaagccc gtgcaggttg tcaggctcga tgaccatgtg cacggtcttc tgaagaaccc
      901 cgccgttatc cctgtctgcg atcttgccgc gggaactgtt gacggacgga gcggcatgct
      961 tgctgtccag tcgtgccgat aaaattgccg gcatgccccc gcacttgtga aagactcccg
     1021 gatacgccct cgaccccatt tactgacggg ggaaaagaca gcagtatcga tgaaaaccca
     1081 atcctccgga gcggcgataa acgggtgttt tttcacagat tctttttgac aatcccctgg
     1141 tattccttta tatttagtta ggccggacca tccagccgca tcatcccgga aaaggagatt
     1201 tttgatgatc cctaccgtcg aatggaaaga cggcatcgta cgcatgctcg accagacgaa
     1261 actgcccctg gagacggtct acacggactg cagggactac cagactgtcg cccgggggat
     1321 caaggaactg tgggtccgcg gtgcgcccgc gatcggggtc tcggctgcca tgggcctggc
     1381 cctgggcgca cgacagatcg ccgccgggag tttcgaggag ttctggccca ggtttgagga
     1441 gatatgcggg cacatggccg cgacgaggcc gaccgcggtc aatctcttct gggccatcga
     1501 gcgcatcaag gcattcgtgc agtccaataa agacaagagt ttggatgaaa tcaggaccat
     1561 ggtggtcgag gaatcccagc gcatgctcga ggaagacatc gcgacgaaca aaaaaatggg
     1621 ggcatatggc gccgccttca tcgccgacgg cgacaatatc atcactcact gcaacgcggg
     1681 ttccatcgcc accgcgggat acggcactgc ggaaggcgtg atgcgcgcgg cgcgggaagc
     1741 ggggaaaaat atccatgtgt acgtagacga gacgcgcccc atgctccagg gggcacgcct
     1801 gaccgcctgg gagctcatgc aggagggcat cccctgcacg ctcattaccg acaacatggc
     1861 cggctacttc atgtaccgtg acatgatcga cctcgccatt gtcggcgctg accgcgtagc
     1921 gcgcaacgga gatgtggcga acaagatcgg cacctacgga ctcgcggtcc tctgcaagga
     1981 gcacggcatc cccttctacg tggcggcgcc ggtctc
//
LOCUS       DASSLD010000123         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1133628, whole genome shotgun sequence.
ACCESSION   DASSLD010000123 DASSLD010000000
VERSION     DASSLD010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133628"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1171)
                     /locus_tag="VFK23_02325"
     CDS             complement(179..1171)
                     /locus_tag="VFK23_02325"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7317950.1"
                     /translation="MTGADNKPAGWRKGLTRNVVVLGFVSLLNDGASEMIYPLLPAFL
                     TSVLGAGPAALGIIEGVAEATASLLKLYSGYLSDRVKRRKVWIVAGYSLSNVIRPLIA
                     FSTSWLHVLVLRFSDRVGKGLRTSPRDAIIADSTPPEFRGKAYGFHRAMDHSGAVIGP
                     LAATALLLVFHDDVKTIFLLSIIPGVLAVTMLLLGLAEKAVAGPRTGTATFNFRSAWA
                     EMTPGFRKYLFIILVFTLGNSTDAFLLLRAQQLGVSVVLLPMIWVVLHVVKMSFSVPG
                     GIISDRIGRKKVIVTGWLVYALVYAGFGFASAHWHAWALFAVYGIFFGLTEGVE"
     gene            1358..1807
                     /locus_tag="VFK23_02330"
     CDS             1358..1807
                     /locus_tag="VFK23_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317951.1"
                     /translation="MPDTVRVSDVENREKLKTYLAELFKKERYAEMTQAIGMHSLLID
                     PQHTMTDQAGLYHYVLDMIKERISKAKGKDYAAWYTGFPKILDQLVSARQIVIDEAAA
                     DELASHRAAYGPFKSLDDFFFWALDDRRLPLEQIVKYVDQTVTLAAC"
     gene            complement(1804..>2105)
                     /locus_tag="VFK23_02335"
     CDS             complement(1804..>2105)
                     /locus_tag="VFK23_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET7317952.1"
                     /translation="KAQTWAGETSLKERAALASLPELFITPDTGPMHLAAAAGARVLA
                     LFGPTAPWRTGPYGPSHVIVRAGRDCSPCFQRTCGRDVVCMSDISVEDVMRKVPF"
BASE COUNT          479 a          652 c          645 g          329 t
ORIGIN      
        1 tcatgcttgc caggagcgcc agcccggcgc ccatgccgaa tgccgccgcc gggccatccc
       61 gctgccaaac caatccgaac aggagcgagg ccggaaaggc gccggcgccg acaaccaggt
      121 ggtagagtcc gaaagacgac ccgcgaagat gggcaggggc gaaatcagcc accagcgcct
      181 attcgacccc ttcggtcagc ccgaaaaaaa taccgtacac cgcgaagagc gcccaggcat
      241 gccagtgcgc ggaggcaaag ccgaatcccg catagaccag ggcatagacc agccagcccg
      301 tcacgataac cttttttctg ccgatgcggt ctgagataat gccgcccgga accgagaagg
      361 acattttgac gacgtgcagg acgacccaga tcatgggcag gagcactacg gaaacgccga
      421 gctgctgggc gcgcagcagc agaaatgcgt ccgtggaatt gcccagcgta aacaccagaa
      481 tgatgaaaag atatttccgg aagccggggg tcatctccgc ccaggcagag cggaaattga
      541 acgtcgccgt tccggtgcga ggacctgcga cggccttttc agcgaggccc aggagaagca
      601 tcgtcacggc gaggacgccc gggatgatcg agagaagaaa aatcgtcttg acgtcgtcat
      661 gaaacaccag gagcagcgcg gtggcggcca gcggcccgat gactgcgccg gagtggtcca
      721 tggcgcggtg gaatccgtag gccttccccc ggaactccgg cggcgtcgaa tcggcgatga
      781 tcgcatcgcg gggcgaggta cggagcccct tgcctacccg gtccgaaaaa cgcagcacga
      841 ggacgtggag ccacgatgtg gaaaaggcga taagcggccg gatcacgttg gacagggaat
      901 aaccggcgac gatccagacc ttgcggcgct tgacgcggtc ggagaggtac ccggagtaca
      961 atttcagcag cgacgccgtc gcctcggcca cgccttcgat gatgccgagg gcggccggac
     1021 ccgctccgag caccgacgtg agaaacgccg gaagcagcgg atagatcatc tccgacgcgc
     1081 cgtcgttcag caggctgacg aagccgagga caacgacgtt tctggtaagg cccttgcgcc
     1141 atccggcagg tttgttgtcg gcaccagtca tgagtagtag cgactttacc cgattcagcc
     1201 tgttttttca agtaaacaat ggagctggcc cgcccggacg cccgcggcgg ggcctgctgc
     1261 cggtaccccg aaattccgct tgcctccgcg ccgcagttgc tatagaatcc cccctgattg
     1321 cgaaatccat cagccgatac caaggaggaa atgcgtaatg ccggataccg tacgggtctc
     1381 agacgttgaa aacagggaaa aactcaagac gtacctggca gagcttttca aaaaggaacg
     1441 ctatgcggaa atgacccagg ccatcggcat gcactcgctc ctgatcgacc cccagcacac
     1501 gatgaccgac caggccggcc tgtaccatta tgtcctggac atgatcaagg agcgcatctc
     1561 caaagccaag ggcaaagact atgcggcgtg gtataccggt ttccccaaga tcctggacca
     1621 gctggtgtcc gcgcggcaga tcgtgatcga cgaggccgca gcggacgaac ttgcatcaca
     1681 ccgtgcggca tacgggccct ttaaatccct cgacgatttc tttttttggg cccttgacga
     1741 ccgccgtctg ccgctcgagc agatcgtaaa gtacgtcgac cagaccgtga cccttgccgc
     1801 ttgctaaaaa gggaccttcc gcatcacgtc ttcaaccgag atgtccgaca tacaaacaac
     1861 atccctgccg cacgtccgct ggaaacaggg ggagcagtct cttcccgcac gcacgatgac
     1921 atgtgacggg ccgtagggcc cggtccgcca cggcgccgtg gggccgaaga gggccaggac
     1981 ccgcgcgccc gcagcggcgg caaggtgcat ggggcccgta tcgggcgtga taaacagctc
     2041 ggggagtgac gcaagggccg cacgttcctt gagcgaggtc tcccctgccc aggtttgagc
     2101 cttct
//
LOCUS       DASSLD010000124         3143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_476940,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000124 DASSLD010000000
VERSION     DASSLD010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476940"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1326
                     /locus_tag="VFK23_02340"
     CDS             121..1326
                     /locus_tag="VFK23_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011176674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7317953.1"
                     /translation="MSTLLSPFTALCTVGFFARLSYALARSPVLPLFALYLGAGPEAI
                     GFAVGISTVTGIFFKLPAGALSDIIGRKRTLLIGLLFFAFMPFTYLWVHSYYPLIIIR
                     FIHGLATAIYGPVSMAVVADIAGAKKGEMLSWFSSITIVGNLLGAPLGGFILHHAAGA
                     AAPSLKEFHYAYLLSGITGMMALVLSLGLLRDGKSDQGGASLREAFKQFVLGIKEVGS
                     DKRVVITSVMEGMQNMAVGALEAFLPIYAVKVAGLNEFQAGILWGVQVVTTILSKPVM
                     GKTSDKYGRTALIVTGLLICAVSFGAIPMLKSFSLLMLAAAFFGLGEAVVTSSGAALV
                     ADLCKEKHFGTAMGTFGTIFDIGHASGPILAGFLIARYDYLYAFWFMAALLVVAVPVF
                     VANVKVSRA"
     gene            1404..2243
                     /locus_tag="VFK23_02345"
     CDS             1404..2243
                     /locus_tag="VFK23_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7317954.1"
                     /translation="MTNGATCPIGGLEKILLATDRSEYSEGAIREAIKFASKCSSRIY
                     ASMTIETNPEYETIGSNVFEKEEGEATAYLDSIKARAVKEGVACETTLHESTDASQAI
                     VDEAAEKKVDMIVIGRHGRKGVAKALMGEVAAKVIAHAPCKVLVVPRAAQIEYRNILV
                     ATDGSAHAVAALNEAIAIAKRCGSRIIALSAMRDETEREEARNLSNKAAETAKKEGVE
                     AEAVTPTGRSFNAIVETAGGRGVDLIVMGTYGKTGLKKVLMGSSTEKVIGNAGCAVLV
                     VKA"
     gene            2263..2595
                     /locus_tag="VFK23_02350"
     CDS             2263..2595
                     /locus_tag="VFK23_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7317955.1"
                     /translation="MKRTITPSELNERPSEFTVIDVRRKADYDGNVILDAEWHNPDDV
                     EEWAKSLPTDKEIVLYCARGGSVSNKVLDSLLAVNISARYIEGGIEGWKQSGGKIAGR
                     NRREGDPL"
     gene            2640..3104
                     /locus_tag="VFK23_02355"
     CDS             2640..3104
                     /locus_tag="VFK23_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HET7317956.1"
                     /translation="MKWITRSHVKVDRVACPWLIKKFVDRDAEFFFVPADLVAAEAVR
                     LGATPFDVPGAELGHNGKECSFEAIMKKFNLSDDPALVLLSRIVNGADTDNTLYKQPE
                     GPGLNAIAEGFRHMNIADDHAINAAEWIVYDALYAYCKEMVAKGKPHGAFAA"
BASE COUNT          735 a          808 c          910 g          690 t
ORIGIN      
        1 agggaatctt taacctctgg tgttgaacca aggcattgtt agcacgcctt cgcgaccttt
       61 gcgccttcac ggttccaagg ttttagttcc ggctgtccgt gttaggagaa gagtacaatc
      121 atgtcaacgt tgttatcacc cttcaccgca ctctgcacgg tgggcttctt cgcccggctg
      181 tcatacgccc tggcgagaag cccggtgctt cctcttttcg cgctttatct tggcgcggga
      241 ccggaagcaa tagggttcgc ggtcggcata tcgaccgtga ccggaatctt cttcaagctt
      301 cccgccggcg ctctctcgga catcatcggc aggaaaagga ccttgctcat cggcctcctg
      361 ttcttcgcct tcatgccctt cacctacctc tgggtgcata gttactatcc gctgatcatc
      421 atcaggttca ttcacgggct tgcgacggca atatatggac ccgtgtcgat ggcggttgtc
      481 gcggatatcg cgggagccaa aaaaggggag atgctttcct ggttctcttc catcaccatc
      541 gtcgggaacc tgctcggagc gccgctcgga ggcttcatac tccatcatgc tgccggcgca
      601 gccgctccat cgctcaagga attccattat gcttatctct tgagcggcat caccggaatg
      661 atggcgcttg ttctctcgct cggtttgctc agggacggaa aatcagacca gggcggcgca
      721 agcttaagag aggcgttcaa acaatttgtt ttagggatta aagaggtcgg cagcgacaag
      781 cgtgttgtga tcacttcggt catggaaggc atgcagaaca tggccgtggg agcactcgaa
      841 gcattcctgc cgatctatgc cgtgaaggta gccgggctca atgaatttca agcgggcatc
      901 ctgtggggtg ttcaagtcgt cacgaccatc ttgtcaaaac cggtcatggg taaaacatct
      961 gacaagtacg gaaggacggc gcttatcgta accggccttt tgatctgcgc ggtttctttc
     1021 ggtgcgatcc cgatgttgaa gagtttctcc ctcctcatgc ttgcggcagc cttcttcggc
     1081 ctgggcgaag ctgtggttac gtcatccggc gcggccctgg ttgcggacct gtgcaaggag
     1141 aagcatttcg gcacggccat gggaaccttc ggcacgatct tcgatatcgg ccacgcatct
     1201 ggtccgattc ttgcgggctt tctcatcgcg cgttatgatt atctctacgc gttctggttc
     1261 atggctgcgc tgctcgtcgt tgctgttccg gtctttgtag cgaatgtgaa ggtcagccga
     1321 gcgtagaatg aaagcgacca attcccggat aggctatact ttcgtttaat gtacagcaaa
     1381 atcgataacc aaaggagacg aaaatgacaa acggagcaac gtgccccatc ggaggactgg
     1441 aaaagattct tttagcaacg gatcgttcgg aatacagcga gggggcgata cgtgaggcga
     1501 tcaaattcgc ctcgaaatgt tcaagcagaa tttacgcgtc catgacgatc gaaaccaatc
     1561 ctgaatacga gaccatcggt tcgaacgtat ttgaaaaaga ggaaggggag gcaactgctt
     1621 atcttgattc catcaaggcg cgtgccgtaa aagaaggcgt cgcatgcgaa acaacgctgc
     1681 atgaatcgac ggatgcttca caagccatcg tggacgaggc tgctgagaag aaagttgaca
     1741 tgatcgtcat cggcaggcac ggtcgcaagg gagtggcgaa ggccctgatg ggtgaagtcg
     1801 ccgcaaaagt gattgcccat gccccctgta aggtgctggt tgtcccaagg gccgcccaga
     1861 tagaatacag aaacattctc gttgccacgg acgggtcggc gcatgccgtt gcagcactga
     1921 acgaggccat cgccattgcg aagcggtgcg ggagcaggat catcgcgctc tccgcgatgc
     1981 gtgatgaaac cgagcgcgaa gaggccagga acctttccaa taaagctgct gagacggcaa
     2041 agaaagaagg agtcgaagcg gaggcggtca ccccgacggg gagatcgttc aatgccatcg
     2101 tcgaaactgc cggcggccgg ggtgtggatc tgatcgtcat ggggacgtac ggaaaaactg
     2161 gcttgaagaa ggtcctcatg ggaagctcta cggaaaaggt tatcgggaac gccggctgcg
     2221 cagtgctcgt ggtcaaggcg tgaaagaacg gggagaattc acatgaaaag aacgattact
     2281 ccaagcgagc ttaatgaaag accatcggaa tttaccgtta tagatgtgag gcggaaggcc
     2341 gactatgatg gaaacgtaat attagatgct gaatggcaca atcctgacga tgttgaagaa
     2401 tgggccaaat cgcttccgac cgataaagaa attgttctct actgcgcaag gggaggatct
     2461 gtgagcaaca aagtcctaga tagcctctta gccgtaaaca ttagtgcccg ttatatcgag
     2521 ggcggtatag aaggatggaa acagagcggc ggcaagatcg cgggaagaaa ccgccgcgaa
     2581 ggcgatcccc tttgaggggc aacttgttct ctgcgcacat tcaattaagg agagcaatca
     2641 tgaaatggat aacccggtca catgtcaagg ttgaccgcgt cgcctgtccc tggctcatca
     2701 aaaagttcgt ggacagggac gccgaattct tttttgtgcc cgcagacctg gtggctgccg
     2761 aggcagtacg cctgggcgcg actcccttcg atgttccggg ggcggagctg gggcacaacg
     2821 gcaaagagtg ctccttcgag gcgatcatga aaaaattcaa cctcagcgac gaccccgcgc
     2881 tcgtactgct ttcccggatc gtgaacggtg cggacacgga caacaccttg tataaacagc
     2941 ccgagggccc gggcttaaac gccattgccg aggggttccg gcacatgaat atcgccgacg
     3001 atcacgctat caatgccgcc gagtggatcg tatacgacgc gctttacgcc tactgcaagg
     3061 agatggtggc aaagggaaaa ccccatggcg catttgcagc gtgacgttgt ccttctcttc
     3121 gcggcgagga gcgtccgcct ctt
//
LOCUS       DASSLD010000125         7492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1128947, whole genome shotgun sequence.
ACCESSION   DASSLD010000125 DASSLD010000000
VERSION     DASSLD010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128947"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VFK23_02360"
     CDS             complement(<1..771)
                     /locus_tag="VFK23_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET7317957.1"
                     /translation="MRFFTIQNIRRITQGLFLLLFLFLFIQTESKGNDELGYPVRLFL
                     DFDPLILVTTLLSAHAAAKAFYFSLITIVITLIFGRVFCGWVCPLGTLHNLVGSIRKK
                     QPIDLSVKGYRVKYYILIAILASAVFTLQPVGIMDPLSLLIRSFSVSVYPLFNYGVRS
                     AFDAVYAVNPAGIAGVSEPVYAVLKKTILSFEQSFYNQGFFIGAIFFIVIGLNFIEKR
                     FWCKYLCPLGALLGILSRFSLLKRSVSEGCTSCGVCATT"
     gene            complement(944..1783)
                     /locus_tag="VFK23_02365"
     CDS             complement(944..1783)
                     /locus_tag="VFK23_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7317958.1"
                     /translation="MIKRKLQQAGAYGSLALFILFSAAPFYVMLITMFKKSHDLFSPT
                     NNPFWLNESPTLEHLDLLFNQTLFPTFLLNTFLVGIAVVVITVLLAAPAALSLSRVAG
                     SWGENVAIGIFLTYLVPPTLLFLPLSRVVSFLGLQNSLWSLVLVYPTFTVPFCTWLLM
                     SFFKAIPRDIEEQAMIDGCSRFSAMVRVILPLSVPGIISVVVFAFTLVAHEYIYALSF
                     IQDSAKKTIGIGMTTELVRGDVFYWGALMGGALIASIPVAILYTMFLDRIILGMAMGT
                     MRK"
     gene            complement(1785..2705)
                     /locus_tag="VFK23_02370"
     CDS             complement(1785..2705)
                     /locus_tag="VFK23_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7317959.1"
                     /translation="MKPGGLADRVAFLASAMLAPVALYIVLLVGFPFVMAFVYSFSIV
                     TVGNPDFHMIGFANFVTVITDPIFRKSLMNTCIFTFTSIGLVLVLSNMLSELLMRELR
                     FKWLIRFLIMLPWTAPIALGAIGFLWFLDSVFSPLDWLLRTWGLLGKPDALFGPMSNL
                     YWLGRPGLAMASVIVVHVWRLLPLATMIRIAGMNSIPPEILEAAQMDGARYYRRLFHI
                     VLPLLLPVISVTVLFSTLFTFTDMTVVWVLTKGGPPDNSTQVLSSLAFLKGIQGGDLA
                     QAAAIALFFFPVLAGISAVVLRIAKKAVVT"
     gene            complement(2702..3703)
                     /locus_tag="VFK23_02375"
     CDS             complement(2702..3703)
                     /locus_tag="VFK23_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7317960.1"
                     /translation="MANVQLIGVKKHFGGVKAVDGIDLEIADGEFLALLGPSGSGKTT
                     LMRLVAGLEQPTGGRIVIDGIVVNDLPPRERGIAMVFQSYALYPHKTVFGNISFPLTV
                     EKMPREDIRKKVEWAANLLGIGHLLDRMPSQVSGGERQRVAIARALVRRPKVLLMDEP
                     LSNLDAKVRHTAREELKLFQREINVTTIYVTHDQAEAMGLGDRIAVMHEGTIRQIGPP
                     EAIYHDPANIFVAGFVGIPPMNFIERGGEVIGFRPEAFLPADFIPEKGSMRFKMQVER
                     VEDLGSHRLVYGTVENTKVIASLSFRSTIAEGGEYDFSVMAQDLKHFDKKSCERVRK"
     gene            complement(3837..5255)
                     /locus_tag="VFK23_02380"
     CDS             complement(3837..5255)
                     /locus_tag="VFK23_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7317961.1"
                     /translation="MKKRITRRDFLKHAGMGAAGLAAGPAFLDKVFAQTPGRRSIRIL
                     QWSHFVPTHDKWYDQYAKQWGEKNNVDVVIDHIGLADLRSTFASEVAAGKGHDIVEFI
                     DPPSDFEPSVLDLTDLNQEAVKRFGKQIPVATRSTYNPNTRKFYGFCHGWTIDPGDYR
                     KSLWEKAGKPNGPTTWEDLLITGAKIKKEQGIQLGIGISQELDTNMAERTLLWSFDSS
                     IQDEHENVVLNNKNTVEAVKFMVELYQKTMTPEVFGWTAASNNQLLLAGKASYILNSI
                     SAYRSAQKDVPEVAKDVFFTPALKGPRGTAWACEHVIYVTVIPKFAEKNADLAKQFLL
                     DRCANYDRAMWESKLYNSPAFFNTTVQTGDRGYPAVAHAKTLRDLHNAWFGSDPFALP
                     GERTDKLLPLKEAEKWSTNIGFPGTANPAEGEIFGTYVLPGMFARVAQGKQTAEESVK
                     QAHAECQKVFDKWRAQGLLGKK"
     gene            complement(5459..6361)
                     /locus_tag="VFK23_02385"
     CDS             complement(5459..6361)
                     /locus_tag="VFK23_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HET7317962.1"
                     /translation="MDRRNFLKLAAMTGAGLSMPWGLEALAGSAGTAGSPDLAVAHGA
                     SPAEIVKAAIDALGGMGKFISRGDVVVVKPNIGWDRTPEQAGNTNPEVVAAVVRLCFE
                     AGAKKVRVFDRPVNDPRRCYVQSGIAPAASALGANVDFVDDRKFRDMAINGEALKSWP
                     LYTELFEADKVINIPIAKHHGSAKLTMSLKNWMGVMGGQRRQIHQKLDQSLVDLSTKI
                     KPTLTILDAVRILTANGPQGGSLEDVKKLDTVIAGVDPVAIDSFGATLFGMKGSDLGY
                     VRLGHERGLGQMDLSKVKIKKISV"
     gene            complement(6365..>7492)
                     /locus_tag="VFK23_02390"
     CDS             complement(6365..>7492)
                     /locus_tag="VFK23_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015849001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7317963.1"
                     /translation="IFRASSLLLAVSPSAPVLIAFRLLQGFGSAMIFGTGMAILTSVF
                     PAAERGRVLGMNVAAVYLGLSLGPTLGGFLTHQFGWRSIFLMNVPLGLLVIFFLATKL
                     KTLEWAEARGERFDFAGALLYSASLVSIMYGLSLLPRRMGIVLTAAGAAGAVMFVLWE
                     RSAESPLLDMDLFFHNATFAFSNLAALINYSATNAVAFLMSLYLQYLKGFTPQGAGMV
                     LVSQPLVMAVFSPLAGRLSDRIEPRVVASIGMAITASGLFLFTFLDESSSVGFDLFGL
                     VLLGFGFALFSSPNTNAVMSSIEKKFYGVGSATLGTMRLTGQMLSMGIAMVIFALSIG
                     SARITPEYYPLFLKSMKTGFVIFSTLCFAGIFASLARGKVR"
BASE COUNT         1590 a         2153 c         2329 g         1420 t
ORIGIN      
        1 tgtggttgcg cagacgccgc aggaggtgca gccttcgctc accgatctct tcaggagaga
       61 gaaccgggac aggatgccca aaagcgcacc cagggggcag agatacttgc accagaaccg
      121 tttctcgatg aagttcaggc cgatgacgat gaaaaatatc gcgccgataa aaaagccctg
      181 gttgtaaaac gactgctcga aggaaaggat ggtttttttg aggaccgcat acaccggttc
      241 ggacacgccg gcgatgcccg ccggatttac cgcatagacc gcgtcgaacg ctgatcgtac
      301 gccgtaattg aacaatggat aaacgctgac ggagaaggag cggatcagga gcgaaagcgg
      361 gtccatgatg cccacgggct ggagcgtaaa gaccgccgag gccaggatgg cgatcaggat
      421 atagtacttg acgcggtatc ccttcacgga gagatcgatc ggttgttttt tcctgatcga
      481 tccgacaagg ttatggagcg tgcccagggg acagacccat ccgcaaaaga ccctgccgaa
      541 gatgagcgtg atcactatgg tgatgaggga aaaataaaac gccttggccg cagcgtgggc
      601 cgagagaagc gtcgttacga gaatgagcgg atcgaaatcg agaaatagcc ggaccgggta
      661 gccgagctcg tcgtttcctt tggactcggt ctggatgaag agaaaaagaa agaggagcag
      721 gaagagccct tgggtaattc tgcggatatt ttggatcgtg aaaaaacgca ttgctcaccg
      781 gactttttaa atctctttca aattgacaat tgatattagc cgcttgtcgg cctgtttgca
      841 acacaaaact gagcaggcat tatcaccctg gatcggaaag gcgcaggtta atctcaatta
      901 ctcccgatag gatattaagg aatccggcag tcactcctct gcgtcacttc cgcatcgtgc
      961 ccattgccat tcccaggatg atgcggtcaa ggaacatcgt ataaaggatc gcgacgggaa
     1021 tgctggcgat cagcgctcca cccatgagcg cgccccagta gaacacgtcg ccccgcacaa
     1081 gttccgtcgt catcccgatg ccgatggtct tcttcgccga gtcctggatg aaggacaggg
     1141 cgtagatgta ctcgtgggct acgagcgtga aagcgaacac gaccacgctg atgatgccgg
     1201 gcacggagag cggcaggatc acccgtacca tggcgctgaa cctgctgcat ccgtcgatca
     1261 tggcctgttc ttcgatgtcc cggggtattg ccttgaagaa gctcatgagg agccaggtgc
     1321 agaacgggac cgtgaacgtg ggatagacga gcacaaggga ccagagtgag ttctgcagtc
     1381 ccaggaacga gaccacgcgg gaaaggggca ggaacagcag cgtcggcggc acgaggtagg
     1441 tgaggaagat gccaatggcc acgttctccc cccaggagcc ggcgacccgg gagaggctga
     1501 gcgcggcggg cgcggcaagg aggaccgtga ttaccacgac ggcaatgccg acgagaaagg
     1561 tgttcagcag gaacgtgggg aacagggtct ggttgaacag caagtcaaga tgctccagcg
     1621 tggggctttc gttcagccag aagggattgt tcgtcgggct gaagaggtca tggctcttct
     1681 tgaacatggt gatgagcatg acgtagaacg gcgcggccga aaacaggatg aacagggcaa
     1741 gcgagccgta ggcgcctgcc tgctgcagct tgcgctttat catatcaggt caccaccgct
     1801 ttcttggcga tccgcagaac cacggcagag atgccggcga gcacgggaaa gaagaacagg
     1861 gcgattgccg cagcctgggc aaggtcccct ccctggatgc ccttcaggaa ggcgaggctc
     1921 gaaaggacct gggtgctgtt gtccggcggc ccgcccttgg tcaggaccca gaccacggtc
     1981 atgtcagtga aggtgaagag cgtggaaaag agcacggtaa cgctgataac cgggagcagc
     2041 agcggcagga cgatatgaaa gagcctgcgg tagtaccttg ccccgtccat ctgcgcggct
     2101 tcgagaatct cgggggggat tgagttcatg ccggcaatgc ggatcatcgt ggccaggggc
     2161 agcaggcgcc agacgtgcac gacgatgacc gaggccatgg caagccccgg cctgccgagc
     2221 cagtacaggt tgctcatggg gccgaagagc gcgtccggct ttccgagaag accccaggtc
     2281 cggagcagcc agtccagggg gctgaacacc gagtcgagga accagagaaa gccgatggcc
     2341 cccagggcga tcggcgcggt ccagggaagc atgatcagga agcggatgag ccacttgaag
     2401 cggagttcgc gcatcaggag ctccgacagc atgttcgaga gcacgagcac caggccgatg
     2461 gaggtgaagg tgaagatgca ggtgttcatg agggacttgc ggaatatggg atcggtgatg
     2521 accgtgacga aatttgcgaa cccgatcatg tggaagtcgg ggtttcccac ggtcacgatg
     2581 ctgaagctgt agacgaaggc catgacgaag gggaagccga cgagcagcac tatgtacagg
     2641 gccaccggcg ccagcatggc tgaggcaagg aacgctacgc ggtccgcaag gccccctggc
     2701 ttcatttccg cacccgttcg caggatttct tgtcgaaatg tttcaggtcc tgcgccatga
     2761 cggaaaagtc gtactcccct ccctcggcaa tggtgctccg gaatgaaaga ctggcaatga
     2821 ccttcgtgtt ctcaacagtg ccatacacca gcctgtgcga cccgaggtct tccacgcgct
     2881 cgacctgcat cttgaagcgc atactgccct tctcgggtat gaaatcggcg ggcaaaaacg
     2941 cctcgggccg gaaaccgatg acctcgccgc ctcgctcgat gaagttcatg ggtggaatgc
     3001 ccacgaaccc ggcgacgaag atgtttgccg ggtcgtggta gatcgcttca ggcggtccga
     3061 tctggcgaat ggtgccctcg tgcatgaccg cgatccggtc gccaagcccc atagcctcgg
     3121 cctggtcgtg ggtcacgtag atggtggtca cgttgatctc gcgctggaac agtttgagct
     3181 cttcgcgcgc cgtgtggcgg accttggcgt cgaggttcga caggggctca tccatgagca
     3241 ggaccttggg acggcgaacc agcgcgcggg cgatggcaac gcgctggcgc tcgccgcccg
     3301 atacctggga tggcatccgg tccagcagat ggccgatgcc gagcagattc gccgcccatt
     3361 ccaccttttt acggatatcc tctctcggca tcttttcaac ggtcagcgga aaggatatgt
     3421 tgccgaagac ggtcttgtgg gggtagaggg cgtagctctg gaacaccatg gcgatgccgc
     3481 gctcacgcgg cgggaggtcg ttcacaacga tgccgtcgat gacgatgcgg ccgcccgtcg
     3541 gctgctcgag gcctgcgacg agcctcatga gcgtggtctt gccgctcccc gaggggccga
     3601 ggagcgccag gaattctccg tccgcgatct ccaggtcgat gccgtcgacc gccttcacac
     3661 cgccgaagtg ctttttaacg ccgatgagtt gaacgttcgc catttaatcc ccgaaaacca
     3721 cgggtcccgg aaatactggg acgcacggta aacggcagtc aagaattaac gcgcctgatg
     3781 ttccctcttt cctcgtttaa ccgtgttcat ccgtggtttc aatccgtacg aagaaactat
     3841 ttcttcccaa gcaggccctg tgcacgccac ttgtcgaaga ccttctggca ctccgcgtgc
     3901 gcctgcttta cggattcctc ggcggtctgt ttgccctggg caacgcgggc gaacatgccc
     3961 ggcaaaacat aggtgccgaa gatctctcct tccgcggggt tcgccgtgcc cgggaagccg
     4021 atattggtgc tccatttttc agcttccttg agcggcagca gtttgtcggt gcgctctccg
     4081 ggaagggcaa agggatcgct gccgaaccac gcgttgtgca ggtcccggag cgtcttggca
     4141 tgggcaacgg cgggatagcc gcggtccccg gtctgcactg tggtgttgaa gaaggccggg
     4201 gagttgtaca gcttgctttc ccacatggcc cggtcgtagt tcgcgcaccg gtcgagcagg
     4261 aactgcttcg caaggtccgc gttcttttcc gcgaacttgg ggatcaccgt aacgtagatc
     4321 acgtgctcgc aggcccatgc cgtgccgcgc ggacccttga gcgcaggggt aaagaacacg
     4381 tccttcgcga cctcgggaac gtccttctgg gcggaacggt aggcggagat ggagttcagg
     4441 atgtacgagg ccttgcccgc gagcaggagc tggttgttcg aggccgcagt ccagccgaag
     4501 acctcgggcg tcatggtctt ctggtacagc tcgaccatga acttcaccgc ttcgacggtg
     4561 ttcttgttat tcagcacgac gttctcgtgc tcgtcctgga tggagctgtc gaaggaccaa
     4621 agcagcgtgc gttcggccat gttggtatcg agctcctggg aaatgccgat gcccagctgg
     4681 atcccctgct ccttcttgat ctttgcgccg gttataagca gatcctccca ggtcgtgggg
     4741 ccgttcggtt tgccggcctt ctcccagagc gatttgcggt agtcgcccgg atcaatggtc
     4801 catccgtggc agaagccgta gaacttcctg gtgttgggat tgtaggtgct gcgggtggcg
     4861 acggggatct gcttgccaaa gcgcttgacg gcttcctggt tcaggtccgt caggtcgagg
     4921 acgctgggtt cgaaatcaga gggagggtcg atgaactcga cgatgtcgtg gcccttgccc
     4981 gcggccactt ccgaggcaaa agtgctccgc aggtcggcga gtccgatgtg gtcgatgacc
     5041 acgtccacat tgttcttctc gccccactgc ttcgcgtact ggtcatacca cttatcgtgg
     5101 gtgggaacga aatggctcca ctggaggatc ctgatggacc ttcggcccgg cgtctgggcg
     5161 aataccttgt cgagaaatgc cggaccggca gcgaggcccg ccgcccccat acccgcgtgc
     5221 ttcagaaaat cgcgccgcgt gatacgcttt ttcatgattc cctcctttgt actggttgag
     5281 atcgtcaaac gcccgaaggc gccttttcct ctcgcgcaaa aaggatgcag gatcgatgaa
     5341 ggcaggactg atgatggtca accgtaccac agccacagcc gcatgtcaat cagcgagcat
     5401 gcgggacgag accatcggga ttacgtgatg ccgtacttct tcagtttcgg agagagcgtt
     5461 aaacacttat tttcttgatc ttcactttac tcagatccat ctgccccagc ccccgctcgt
     5521 gtccgagccg cacatacccg aggtcgctcc ccttcatgcc gaagagcgtc gcgccgaagg
     5581 aatctattgc caccgggtcc acgccggcga tgacggtgtc gagcttcttt acgtcctcga
     5641 ggctcccgcc ctggggcccg tttgccgtca ggatgcgcac ggcgtcaagg atggtgagcg
     5701 tgggcttgat cttcgtcgag aggtccacga ggctctggtc gagcttctgg tggatctgtc
     5761 ttctctggcc tcccatcact cccatccagt tcttcaggga catcgtcaat tttgccgagc
     5821 cgtggtgttt cgcgatgggg atgttgatta ccttgtccgc ctcgaagagc tccgtataga
     5881 gcggccagga cttgagcgcc tcgccgttta tggccatgtc cctgaacttc cggtcatcga
     5941 caaagtcgac gttcgcgccc agtgcggacg ctgcaggagc aatgccgctc tggacgtagc
     6001 accgccgcgg atcgttcacc ggccggtcga acacccgcac cttcttggcg ccggcctcga
     6061 agcagagcct gaccaccgcg gcgaccacct cgggattggt gttgccggcc tgctccggtg
     6121 tccggtccca gccgatgttc ggcttgacga ccaccacatc tccccgggaa atgaacttgc
     6181 ccatgcctcc caaggcgtca attgccgctt tcacgatctc tgcgggagac gcgccgtgtg
     6241 cgactgcaag gtcgggactg cctgcggtcc cggcggaccc ggcaagcgcc tccagccccc
     6301 agggcatcga aagtcccgcg ccggtcatcg cggcaagctt cagaaaattc cttcggtcca
     6361 cctctcacct cactttccct cttgccagcg aagcaaaaat gccggcgaaa cagagggtgg
     6421 aaaaaataac gaaaccggtc ttcatgcttt tgagaaagag cgggtaatat tccggggtga
     6481 tccgcgcgct tccgatggac agagcgaaaa taaccatcgc gatgcccata gacagcatct
     6541 ggcccgtcag gcgcatggtg ccgagcgtcg ccgagcccac gccgtagaac ttcttttcga
     6601 tggaactcat gaccgcgttc gtgttcggcg acgagaacag ggcgaagccg aagcccagga
     6661 ggaccagacc gaacagatcg aatcccacgg aagaactctc gtccaagaag gtaaacagaa
     6721 aaagcccgga ggcggtgata gccatgccga tggacgcgac gaccctcggc tctatcctgt
     6781 cggaaagccg gccagccagg ggggaaaaaa ccgccatgac caggggctgc gacacgagga
     6841 ccatgcccgc tccctggggt gtgaaacctt tgaggtactg gaggtagagg ctcatgagga
     6901 acgcaacggc attggtcgcg ctgtagttga tcagcgccgc gagattcgag aaggcgaagg
     6961 tcgcgttatg aaagaagagg tccatgtcaa ggagcgggct ctccgccgac ctttcccaga
     7021 gaacgaacat caccgctccc gccgcgccgg ctgcggtcag gacgatcccc atcctgcgcg
     7081 ggagaagcga gagcccgtac atgatggaga ccagcgaggc gctgtagagg agggcgcccg
     7141 caaagtcgaa ccgctctccc ctggcttcgg cccactctag ggttttcaac ttcgttgcca
     7201 gaaaaaagat caccagcaag ccgagcggca cgttcatgag aaagatgctc cgccagccga
     7261 actgatgggt caggaacccg cccaaggtcg gaccgagcga cagtccgagg tagacggccg
     7321 ccacattcat gccgagcacg cggccgcgct ccgctgcggg gaacaccgaa gtcaggatcg
     7381 ccatgccggt cccgaagatc atggcgctgc cgaatccctg gagcaagcga aacgcgatga
     7441 gcaccggcgc ggacggcgac accgcgagca gaagcgatga ggccctgaag at
//
LOCUS       DASSLD010000126         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_314062,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000126 DASSLD010000000
VERSION     DASSLD010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314062"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VFK23_02395"
     CDS             complement(<1..259)
                     /locus_tag="VFK23_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317964.1"
                     /translation="MDSQQRSMLTYLGVPIERPNVAFFELTSCEGCQLQIVNNEATLF
                     NFLSLVNIVNFREVMTEKTDAFDIAFVEGSVTRADEVERLNL"
     gene            complement(259..1164)
                     /locus_tag="VFK23_02400"
     CDS             complement(259..1164)
                     /locus_tag="VFK23_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HET7317965.1"
                     /translation="MNTRLKFTDVVRDSRGYFRKNDLAIFEHSSKAEITSVHHLTAME
                     NLYRIRIINGEERKRFVFKPGQFLMLELPGIGEAPFAISSSPSSHGDVELCIRKIGNL
                     TNFLSRAGRGVRVGIRGPFGTSFPMEEMHGQNILLIAGGLGLAPLRSPIAYVQENRNL
                     FDNVNIIYGTKEPAQLLFTYQYDTWRKDDIELDIIVEKADHTWKGPVGLTTKILEHMA
                     SKSDKLIPSRTYAVVCGPPVMFKSVCNMLSEIGIPMQKMFVSLERRMHCGMGKCCRCN
                     VGSTYTCLSGPVFDYWTVMNLKEAI"
     gene            complement(1161..>2016)
                     /locus_tag="VFK23_02405"
     CDS             complement(1161..>2016)
                     /locus_tag="VFK23_02405"
                     /inference="COORDINATES: protein motif:HMM:NF028488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7317966.1"
                     /translation="YFLPYREDLATFEIEGSDWKKTVDYHCKAPTVFCGLHACDINAL
                     NRLDKVLMGNVYPMPYYAVKRRNTFIIGIDCRPQPFCFCRSMGADTVLHGFDMFVTDI
                     GGRYFVEILSAAAYDVLRSVSTRAPEEEDHRQYMKAVAEKDEMFTAHVDTTDLTKILD
                     MEFQSPVWNDWGDKCLGCGTCANVCPTCYCYGVEETVNVDLRSARKTKILYSCNLIDF
                     AEVAGGHNFRPERSSRLKYRYYHKHRGFVEAYEESLCVGCGRCGEACLAGITVPNVIA
                     SVRREGLK"
BASE COUNT          415 a          567 c          587 g          447 t
ORIGIN      
        1 ccagattgag acgctcgacc tcgtcggccc gggtgacgct cccttcgaca aaggcgatat
       61 cgaaggcgtc ggtcttctcg gtcatgacct cccggaagtt cacgatgttc accagggaga
      121 gaaaattaaa gagcgtcgcc tcgttgttca caatctggag ctggcatccc tcacaggacg
      181 taagttcgaa aaaagcgacg ttcgggcgct caatcgggac cccgagatag gttagcatcg
      241 atcgttgctg actgtccatc agatggcctc tttcaggttc atcacggtcc agtagtcgaa
      301 caccggaccg gaaagacacg tataggtgga ccccacgttg cagcggcagc actttcccat
      361 tccgcagtgc atgcgacgct ccagggagac gaacatcttc tgcatgggga ttccgatttc
      421 agacagcata ttgcatacgg acttgaacat gaccggcggg ccgcaaacga cggcgtaggt
      481 tctgctcggg atcagtttat cgcttttcga tgccatgtgt tcgagtatct tcgttgttag
      541 gcccacaggt cctttccagg tgtggtctgc cttctctacg ataatgtcca gctctatgtc
      601 gtccttgcgc caggtgtcgt actggtaggt gaaaagaagc tgcgccggtt ccttcgtccc
      661 gtagatgatg ttcacgttat cgaacagatt gcggttttcc tggacgtagg cgatcggcga
      721 acgcaggggg gcaaggccga ggccgccggc gatcaggagt atgttctgcc cgtgcatctc
      781 ctccatgggg aagctggtcc cgaagggacc ccgaatcccg actcgcacgc cgcggccggc
      841 ccgggacagg aagttagtga ggttgccgat tttgcgaatg cagagttcca cgtccccgtg
      901 gctactcggg gaagaggata tggcaaaggg ggcctcgccg atgcccggaa gctccagcat
      961 caggaactgg cccggcttga agacaaaccg tttacgctct tctccgttga tgatgcgtat
     1021 gcgatagagg ttctccatgg cggtgagatg gtgaacactc gtgatctccg ccttgctgga
     1081 atgttcaaag atcgcgaggt cgtttttccg gaagtatccg cgggagtccc ggacgacatc
     1141 ggtgaacttc agccgcgtat tcacttaagc ccctctcttc gcacgcttgc gatgacgttc
     1201 ggcacggtaa tgccggccag gcaagcctca ccgcagcgtc cgcaacccac gcagagcgac
     1261 tcctcgtacg cctctacgaa gccgcggtgt ttatgatagt agcggtactt gagccgggat
     1321 gacctctcgg gcctgaaatt gtgtccaccc gcgacctcgg caaaatcgat aaggttgcag
     1381 gaatagagaa tctttgtctt ccgcgcgctc ctgaggtcga cgttcacggt ttcttccacg
     1441 ccgtagcaat agcacgtcgg gcagacgttc gcgcacgttc cgcagcccag gcacttatct
     1501 ccccaatcat tccagacggg agactggaac tccatgtcaa gaatcttggt caggtccgtc
     1561 gtgtcgacgt gcgccgtgaa catctcgtcc ttctccgcga cggccttcat atattgcctg
     1621 tgatcttcct cttccggcgc acgcgtcgaa acgctgcgga ggacgtcgta ggcagcggcg
     1681 gaaaggatct cgacgaagta ccggccgccg atatcggtta caaacatgtc aaagccgtgg
     1741 agcacggtgt ccgcacccat ggaccggcag aagcagaagg gctgaggacg gcagtcgatg
     1801 ccgatgatga acgtgttcct gcgctttacg gcatagtagg gcataggata gacattgccc
     1861 attaatacct tgtcgagcct gttgagcgcg ttgatgtcgc atgcgtgaag gccgcagaac
     1921 accgtgggtg ccttgcagtg gtagtccacg gtcttcttcc agtcggagcc ttcgatctcg
     1981 aatgtcgcta ggtcttcccg gtacgggaga aaatat
//
LOCUS       DASSLD010000127         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_737531,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000127 DASSLD010000000
VERSION     DASSLD010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737531"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFK23_02410"
     CDS             <1..479
                     /locus_tag="VFK23_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7317967.1"
                     /translation="ENVPALVGLGKAAELAAAEVLHRMGYVAPLRDRLQKGLMEKIDH
                     LVINGHPKRRLPHNLNVSMWYVEGESMLLFLNMEGISVSSGSACTSRSLKSSHVLACI
                     GTDAAVANGTLLLTLGMGNTAEDIDYVVEKLPPIVQRLREMSPLYEDMIKKEAARK"
     gene            523..951
                     /gene="nifU"
                     /locus_tag="VFK23_02415"
     CDS             523..951
                     /gene="nifU"
                     /locus_tag="VFK23_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HET7317968.1"
                     /translation="MEQYSSKVMDHFAHPHNVGEIENADGVGTVGNPVCGDVMRLYIK
                     VEGNVITDAKFKTFGCGAAIATSSMVTDLVKGKTIDEALSISNAAVAEALGGLPKVKM
                     HCSVLAEEALKSALDDYYKKKGLTSPVKMPAEAGHEHEIG"
     gene            1072..1314
                     /locus_tag="VFK23_02420"
     CDS             1072..1314
                     /locus_tag="VFK23_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012963936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HET7317969.1"
                     /translation="MDIKDIKPDAVLDTVGLACPMPIYKASGRIKDMEPGQVLEVQSD
                     DEGIEKDMPAWCRLTGNEFLGLMRENGEYRVYVRKK"
     gene            1445..2503
                     /locus_tag="VFK23_02425"
     CDS             1445..2503
                     /locus_tag="VFK23_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7317970.1"
                     /translation="MQTISSLKEGDWVEEIYLVTSKQVSTAKNGVTYLALKLADKSGE
                     IDGRLWDNADEVAGKFEREDFVRIKGMASNYQGSMQIKMKTLEKVDDSRVDMANFLES
                     SPRNIDEMVRELRTVAASVKNPHLRKLMDSFLNDTAFMAVFRQAPAAKTLHHNYIGGL
                     LEHVVELMDLIRDLAKHFPSADADLLTAGAFLHDIGKVRELTIKKSIGYTTEGRLLGH
                     ISLGYEMVTEKVNAIPGFPGELAMLLKHIMLSHHGEYEFGSPKRPKIQEAIIINYLDD
                     LSAKINNFQATLKKENVAAGEWTGFSKMHDRYLYRQTACEGMAGEAAETEEMRPPGVK
                     AGRGSHGADRSGKLPLDI"
BASE COUNT          619 a          715 c          795 g          482 t
ORIGIN      
        1 ccgagaacgt gccggccctc gtggggctgg gcaaggccgc ggagcttgcc gcggcggaag
       61 tcctgcaccg gatgggctac gtcgcgccgc tccgggaccg gctgcagaag gggctcatgg
      121 aaaagattga ccatctggtc atcaacggcc acccgaagcg gcggctgccg cacaatctga
      181 acgtctccat gtggtatgtc gagggggaat cgatgcttct gttcctcaac atggaaggga
      241 tctcggtctc gagcggctcg gcgtgcacgt cgcgctcgct gaaatcgtcg cacgtgcttg
      301 cgtgcatcgg gaccgacgcc gcggtcgcga acggaacgct gctcctgacg ctcggcatgg
      361 gaaataccgc ggaagacata gactacgtgg tagagaaact gcctcccatc gttcaacggc
      421 tgcgggagat gtcgcctctc tacgaagata tgatcaagaa ggaagcggcc aggaaataaa
      481 accagcggcg ggcctgagcc cgcggctaca acggaggatc atatggaaca gtacagttcg
      541 aaggtgatgg atcatttcgc ccacccgcac aacgtgggag agatcgagaa tgccgacggc
      601 gtaggcaccg tgggaaatcc tgtctgtgga gatgtcatgc ggctctacat caaggtcgag
      661 ggcaacgtca ttaccgatgc caagttcaag accttcgggt gcggcgcggc catcgccacg
      721 agcagcatgg tgacggatct ggtaaagggg aagaccatcg atgaggcact ctccatatcg
      781 aacgcagcgg tggcggaggc gctcggcggg ctgccgaaag tcaagatgca ctgttccgtg
      841 cttgccgaag aggcgctcaa atccgccctc gacgactatt acaagaagaa aggcctcacc
      901 tcccccgtga agatgcccgc ggaagcgggg cacgagcacg agataggatg attcgattgg
      961 gaatgtgtcg gcttatggcg cgtagctcag aggaaaagcc gcgactatga gctacaagct
     1021 gtgagatcaa tttatacgta tctatccgtg gcgcagataa aggctgtatc aatggatatc
     1081 aaagacatca aacccgacgc cgtgctggac accgtaggtc tcgcctgtcc catgccgatc
     1141 tacaaggcct ctggaaggat caaggacatg gagccgggac aggttctcga agtgcagtca
     1201 gacgacgaag gaatcgaaaa ggacatgccc gcatggtgca ggctgacggg caatgaattt
     1261 ctcggtctca tgcgtgagaa cggtgagtac cgcgtttacg tgagaaagaa gtagaacccc
     1321 ccgctttgtc cgatcccccc tgttaccgtt gcatatattg atcgggccgc agccattgcg
     1381 tccgctttcc gtttgacaac ccagggtcga tagtttatta tcacagcacg aaaccggagg
     1441 aacaatgcag accatatcgt ccctgaaaga aggcgactgg gtcgaggaga tatacctcgt
     1501 cacgtcaaag caggtgtcga cggccaagaa cggcgtgacc tacctggcgc tgaagctcgc
     1561 ggacaaatcc ggcgagatcg acggcaggct gtgggacaac gccgatgaag tggccgggaa
     1621 gttcgaacga gaggatttcg tccgcatcaa ggggatggct tcgaactacc agggctcgat
     1681 gcagatcaag atgaagacgc tcgagaaagt cgacgactcc cgggttgaca tggccaactt
     1741 cctcgagtcc tccccgcgga acatcgacga aatggtgagg gagctcagga ctgttgccgc
     1801 ttcggtgaag aaccctcatc tccggaagct gatggactcc ttcctgaacg atacggcatt
     1861 catggccgtt ttcaggcaag ctccggccgc caagacgctc caccacaatt acatcggcgg
     1921 cctgctcgag catgttgtgg aactgatgga cctcatccgc gatctggcaa aacactttcc
     1981 ttcggccgac gcagacctgc tgaccgcggg ggcctttctg cacgacatcg gcaaggtcag
     2041 ggaactcacg atcaagaagt ccatcgggta cacgaccgaa gggcgcttgc tggggcacat
     2101 atcgctcggc tatgaaatgg tcaccgagaa ggtgaacgcc attcccggtt ttcccgggga
     2161 actcgccatg ctgctgaagc acatcatgct ctcgcatcac ggagagtacg agttcggatc
     2221 gcccaagcgg cccaagatcc aggaagccat cattatcaat tatctcgacg acctttcggc
     2281 aaagatcaac aacttccagg cgaccctgaa gaaggagaac gtggctgccg gcgagtggac
     2341 ggggttcagc aagatgcacg accgttacct gtaccggcag accgcctgcg aagggatggc
     2401 cggagaagcg gcggaaaccg aggaaatgcg gccccccggc gtcaaggcgg ggcgcggcag
     2461 tcacggggcg gatcgaagcg gcaagctgcc gctggatatt tgactaaatt caggccagga
     2521 gtcggaagcc aggagtcagc gagaaatctt ttgtgttctt ctgtattctg aattctgact
     2581 cctgactccc tcttttgagg tattcatgtt c
//
LOCUS       DASSLD010000128         3917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1362381, whole genome shotgun sequence.
ACCESSION   DASSLD010000128 DASSLD010000000
VERSION     DASSLD010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362381"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="VFK23_02430"
     CDS             complement(<1..1196)
                     /locus_tag="VFK23_02430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7317971.1"
                     /translation="MQNLHVISWNVTRSCNLACSHCYLPAAAGKATGPATSFNELTTR
                     EAFLLIDRISLVNPEVMLILSGGEPLLREDLFELSTYATGKGMMVVLGSNGLLIDDAA
                     AKKMRQSGVSGISISLDSVGPEIHDSVRSCAGAWEKAVAAIRRCRDAGLAVQINAVVT
                     TKNYNEMPALISFSRSLGAKVFSPFFLVCTGRGEKLTDLSPAQYEQVLSRIVEEQKRY
                     DDMKIRTRCAPTLRRILNEKNPRSSLLKTGAGQCRAGSSYCRISPEGDVTACPYMPVS
                     AGNVREREFKDIWEHSELFDSLRNPALKGKCGRCVFRLLCGGCRARAYAAYKDHLAED
                     PWCAHVPAGGEVIQPPSFGEDSNISSGKTLKPVWTAEAEDRLKKVPSFVRAMVRSAVE
                     RYASEN"
     gene            complement(1204..2199)
                     /locus_tag="VFK23_02435"
     CDS             complement(1204..2199)
                     /locus_tag="VFK23_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7317972.1"
                     /translation="MFKNIFIPIDNSKYSRYCTVIGTALAGQFGSQLTGSHVYSANLH
                     DRRFRDMETGLPGHYREEERLKKSRKVHDSLIGDGLRLISDAYLENFKQACRNSAVPF
                     NCKMVEGKNWLELVKDVRESGYDLVILGILGLGAMNGNLIGSVCERVVRKVGADVLVV
                     KSDTPLSGRVVVAIDGSEHSYSALHKALALAHKFDFTIEAVSAYDPFFHRRAFQALVG
                     VLSDEAGKMFRFKDQEKLHDEIIDDGLGKIYRDYLDRAVRMGQDEDIVVKSTLLAGKA
                     YDEIKKYLEKKPPSLLVVGRFGAHKTDEQDIGSTAENLLRLAPCNVLISGGVPEE"
     gene            complement(2393..3823)
                     /locus_tag="VFK23_02440"
     CDS             complement(2393..3823)
                     /locus_tag="VFK23_02440"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7317973.1"
                     /translation="MRFLPALIILAMLPATALPQEKGKVLYDQWCSQCHGEKGDGKGP
                     AADFVFPKPRDFTKGTYKFHSTASGEPPLDSDIIRSVRNGNPGTSMPPWKRFSDEEVK
                     AMVDYLKKFSAPDTFETKGTAYKVSPPSGSKEKLIAQGKELYEKTKCWECHGKAGRGD
                     GEKGWQEKFKDDWGNKIYPVDQTSPWEYRNGTGVNDIFVTISAGIEGTPMTSYGDTVP
                     EEKRWALAYYVKSEQKTRNLGLSLRVKKVEAVPSSTDDPVWQSVPALDLPMAGQVIFE
                     PRDFTTLITNARVRGVYTGSDVAILIEWTGKKHNTGDDGNPPDAARIQFPSKTPEGAL
                     PYFFMGDRKLGVNIWQWKASDNLGVRLIANGPDNLSQPETQDMKVTASYTDGLYRVMF
                     TRQLQAGDKNDSVFEVGKFTPFSIALYDGRSNEQDRKGAVSAWYYLVLEPPTPATVYI
                     FPAAAFIVFLGIGLLLHKMVRRKGQH"
BASE COUNT          778 a         1148 c         1058 g          933 t
ORIGIN      
        1 tcgttttcac tcgcataccg ttccaccgcg cttctgacca tggcgcggac aaacgagggg
       61 actttcttga gccgatcctc ggcctccgcg gtccagacgg gtttcaacgt cttgccggat
      121 gaaatgttcg aatcctcgcc aaaagagggt ggctgtatca cttcgcctcc ggcggggaca
      181 tgtgcgcacc agggatcttc cgcaagatga tctttataag cggcataagc cctcgccctg
      241 caccctccgc agagaaggcg gaagacgcac cgaccacatt tgcctttgag agcgggattt
      301 ctcaacgagt cgaagagctc cgaatgttcc catatatctt taaattcccg ttctctcacg
      361 ttgcccgctg aaacaggcat atacggacag gcggtaacat cgccttcggg cgagatcctg
      421 caataggatg atcctgcgcg gcattgcccg gcacccgtct tgaggagcga agatcggggg
      481 tttttttcat tcaaaatcct tctcaaggta ggggcgcacc gtgtccgtat tttcatgtca
      541 tcatatctct tctgttcttc tacgatgcgt gacagaacct gttcgtactg agcgggagaa
      601 aggtccgtca gtttctcacc tctgccggta cagacgagga aaaaggggct gaagactttt
      661 gccccgaggc tgcgggaaaa ggaaatcaac gccggcatct cgttatagtt ctttgtcgtt
      721 acgaccgcgt tgatctgaac ggctaacccc gcatccctgc agcgccgtat cgcggcaaca
      781 gctttttccc aggcacccgc gcaggaacgg acggagtcgt gaatctcggg accgaccgaa
      841 tccaggctga tactgatccc cgaaaccccg ctctgtctca ttttcttcgc tgccgcatca
      901 tcaataagga gcccgttcga accgagaacg accatcatcc ccttgccggt cgcatatgtt
      961 gaaagctcga agaggtcctc ccggagcagg ggctcgccgc ccgaaaggat cagcatgacc
     1021 tcggggttga cgagagaaat ccggtcgatg agcagaaagg cctctcttgt cgtcagctca
     1081 ttgaaggaag tcgcggggcc ggttgctttt cccgccgccg cggggagata gcaatggctg
     1141 cacgcgagat tgcagctcct ggtcacattc caggatatga catggagatt ttgcatagga
     1201 ttatcattcc tccggaacgc cgccgctgat cagcacattg cagggggcga ggcggagcag
     1261 gttctcggcg gtgctgccga tgtcctgctc atcggtcttg tgcgcgccga agcggcctac
     1321 gaccagaaga gacggcggtt ttttttccag atactttttt atttcgtcgt aggcctttcc
     1381 tgcgagaagc gtgctcttca ccacgatgtc ttcatcctgc cccatccgga ccgctcgatc
     1441 gaggtaatcc ctgtagatct tgcccaggcc gtcgtcgatg atctcgtcat gcagtttttc
     1501 ctggtccttg aacctgaaca tcttccctgc ctcgtctgac aggacgccga cgagcgcctg
     1561 aaaggccctc cggtggaaga agggatcata cgcggaaacg gcctctatcg tgaagtcgaa
     1621 cttatgtgcc agggccagcg ccttgtgaag agcgctgtag gaatgctcgc tcccatcgat
     1681 ggccacgacc acccgccccg aaagaggtgt gtcgctcttg accaccagga cgtctgcccc
     1741 gactttccgc acgacgcgct cacagacgct tccgatgagg ttcccgttca tcgcgccgag
     1801 acccaggatc cccaggatca caagatcata accgctctcg cgcacgtctt tcaccagttc
     1861 cagccagttc ttaccttcca ccattttgca gttgaacggg accgctgaat tgcggcacgc
     1921 ctgcttgaag ttttccagat aggcgtcgga gatcaaccgc aggccgtccc cgatgaggga
     1981 gtcgtgtacc tttcgcgact tcttcagtct ttcttcctcc cggtaatggc cggggagccc
     2041 cgtttccatg tccctgaacc gcctgtcgtg cagattcgcg ctgtagacgt ggctgccggt
     2101 cagttgcgag ccgaattgcc cggccagggc cgttccgatc acggtacagt aacgggaata
     2161 tttcgagttg tcgatgggga tgaatatatt tttaaacatt gaaggggcta cctcgcctta
     2221 cttcacccgg ataaaccgga gccaagaagc aaaccagcgg cgtctctcgc agagcacgca
     2281 gagtgaaatc aaaaactgga cttcaattta aaacggggat gatttggatc tatctctgtg
     2341 cgataaattc tttccattct gagaagggcc tcattcgcaa ctgactgagc agttaatgtt
     2401 gcccttttct ccggaccatt ttatgcagca gcagaccgat gccgagaaaa acgataaacg
     2461 ccgcagcagg gaagatatac actgtagccg gcgtcggtgg ctccagcacc aggtaatacc
     2521 atgcggaaac cgcgcctttc ctgtcctgct cgttgctccg tccgtcgtaa agggcgattg
     2581 agaagggtgt gaattttccg acctcgaaga cggagtcgtt cttgtccccg gcttgaagct
     2641 gccgagtgaa catgactcgg tacaggccgt ccgtatatga cgccgtgacc ttcatgtctt
     2701 gtgtttccgg ctgcgacaga ttatccgggc cgttcgcaat gagcctgacc cccaaattgt
     2761 ccgacgcctt ccactgccag atgttgacac cgagtttcct gtctcccatg aaaaagtacg
     2821 gcagggcgcc ttccggcgtc ttcgacggga actgaatgcg cgcggcgtcg ggaggatttc
     2881 cgtcgtcgcc ggtattgtgc tttttccccg tccattcgat cagtatcgcg acatcggaac
     2941 ctgtgtagac cccccgcacc cgggcgttcg ttatgagggt cgtaaagtcg cgcggttcga
     3001 agatcacctg gcccgccatc ggaaggtcga gagcggggac gctctgccag acgggatcat
     3061 cggtcgaaga ggggacggcc tccacttttt ttacccgcag ggaaagcccg aggtttctcg
     3121 tcttctgctc agacttgaca taataggcga gcgcccaccg cttctcttcc gggaccgtgt
     3181 ccccgtagga ggtcatgggc gtgccctcga tgcccgctga aatggtaacg aatatgtcat
     3241 taacgccggt gccgttccgg tattcccagg ggctcgtctg gtcgacggga tatatcttgt
     3301 ttccccagtc gtccttgaat ttttcctgcc agcccttctc gccgtctccc cgtccggcct
     3361 ttccgtggca ttcccagcac ttcgtctttt catacagctc ttttccctgg gcgatcagtt
     3421 tctccttgct gccggaaggc gggctcacct tgtacgcggt acccttcgtt tcgaaggtgt
     3481 cgggcgccga aaattttttc agataatcga ccatagcctt cacctcctcg tcgctgaaac
     3541 gtttccaggg cggcatggag gtgccggggt ttccgttcct gaccgaccgg atgatgtccg
     3601 aatccagcgg cggctctccg gaggcggtgg agtggaattt atacgtgcct ttcgtgaagt
     3661 cccgcggctt cggaaaaacg aaatcagcgg ccggaccctt gccgtcgcct ttttccccgt
     3721 gacactggct gcaccactgg tcgtagagga ccttcccctt ttcttgggga agtgctgtgg
     3781 cgggcagcat tgccagaatg ataagtgctg ggagaaacct catgatgaaa tttccctttc
     3841 tgatttacca tgtcctcggt ttgttgccgg tagcgtcata gataaagagt atcaccttcc
     3901 acatctgctc ttccttt
//
LOCUS       DASSLD010000129         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1487609, whole genome shotgun sequence.
ACCESSION   DASSLD010000129 DASSLD010000000
VERSION     DASSLD010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487609"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1553
                     /gene="mutS"
                     /locus_tag="VFK23_02445"
     CDS             <1..1553
                     /gene="mutS"
                     /locus_tag="VFK23_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET7317974.1"
                     /translation="ALKQSFLSMPKIRGMLEDSRAKLIFDLASEWDHLGDLYQLIDKA
                     IYDDPPFTLREGRLIKKGHDAGLDELRAISSEGKGFIAGIEQRERERTGIGSLKVSYN
                     RVFGYYIEVTKANLANVPQDFIRKQTLANAERFITPELKEYEEKVLGAEEKILDMEYR
                     LFQEVRESVAAQTVRVQDMARRISVLDALASLAEAAARGGYARPAIVDGDTLRIVEGR
                     HPVLERLDAQERFIPNDALLDCEANQLIILTGPNMAGKSTYMRQVAVIALMAQMGSFV
                     PAKEAQVGLVDRIFTRVGASDFISRGQSTFMVEMNETANILNNATDRSLIILDEIGRG
                     TSTFDGISIAWAVAEYIHTKLRARTLFATHYHELTELALTMDRVKNCTVAIKEWNDQI
                     IFLRRVTPGGADKSYGIQVARLAGLPQAVIARAKEVLANLEKAEFNDLGEPVAAPRTG
                     KGERENGGRGEKTGETAVEGTEPQLGLFASEAGLLFKELQDMDLDRMTPLEAMIKLQE
                     LKKRVEGS"
     gene            1734..2186
                     /gene="rplI"
                     /locus_tag="VFK23_02450"
     CDS             1734..2186
                     /gene="rplI"
                     /locus_tag="VFK23_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET7317975.1"
                     /translation="MQVILREDIEKLGKIGDMVKVADGYARNYLVPTKKAIEATPKNV
                     HAMEHAKKMVADRLRKLKKEAAADADNIKALAVTIKAKAGEEGKLFGSVTTMDIAEAA
                     KAQGVVIDKRKIALEEPIKRLGDYTVTVKLHADITAEFKVSVIAEESA"
     gene            2295..>2754
                     /locus_tag="VFK23_02455"
     CDS             2295..>2754
                     /locus_tag="VFK23_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HET7317976.1"
                     /translation="MSVETEVSLQKLPPQNIEAEQMVLGAVLIENDSINNVAAILSPD
                     DFYKDIHRRIFSVMLEMFERREAIDLVTLTDCLRGKVALESVGGASYLASLVSLVPTA
                     ANIKYHAKIIREKSVLRRLIHASTDIITQCYDDSRTVDSIDELLNTAERSI"
BASE COUNT          647 a          809 c          831 g          467 t
ORIGIN      
        1 tggcgctgaa gcaatccttc ctctcgatgc cgaaaatacg agggatgctg gaggactccc
       61 gggcaaagtt gattttcgac ctcgcgtcgg agtgggacca tctgggggat ctgtaccagc
      121 tcattgacaa ggccatttac gacgaccccc cctttacgct ccgggagggc agactgatca
      181 agaaaggaca tgacgccggg ctcgacgaac tgcgcgccat cagttccgaa ggcaagggtt
      241 ttatcgcggg catcgagcag cgcgagcggg agcgcacggg gataggctct ctcaaggtga
      301 gctataacag ggtcttcggc tactacatcg aagtgaccaa agcgaacctc gcgaatgtgc
      361 cgcaggattt catccgcaag cagacgcttg cgaacgccga gaggttcatc actccggagc
      421 tcaaggaata cgaagaaaag gtgctgggcg ccgaggagaa gatcctcgac atggagtacc
      481 ggctttttca ggaagtacgg gagtcggtcg cggctcagac ggtccgcgtt caggacatgg
      541 cgaggaggat ttcggttctc gacgcccttg cctcgctcgc cgaagccgcg gccaggggcg
      601 gctacgcaag gcccgcgatc gtggacggcg acaccctcag gatcgtcgag gggcgtcacc
      661 cggtgctcga acggttagac gcgcaggagc gcttcatccc gaacgacgcc ctgctcgact
      721 gcgaggcgaa ccagctcatc atcctcacgg ggccgaacat ggccggaaag tccacctata
      781 tgcgccaggt cgccgtgatc gcgctcatgg cccagatggg gagctttgtg ccggccaaag
      841 aggcgcaggt gggcctcgtg gaccggatct tcacgcgcgt cggggcatcg gacttcatct
      901 cccgcggcca gagcaccttc atggtcgaga tgaacgagac ggccaacatc ctgaacaacg
      961 ccacggacag gagcctcatc atcctggacg agatcgggag gggcacctct accttcgacg
     1021 gcatcagcat tgcctgggcg gtggcggaat atatccacac gaagctcaga gcgcggaccc
     1081 tcttcgccac gcattaccat gagctcacgg aactcgctct caccatggac cgggtgaaga
     1141 actgcaccgt ggccatcaag gaatggaacg accagatcat attcctccgc cgcgtcaccc
     1201 ccggcggtgc agacaagtcc tacggcatcc aggtcgcgag actcgcggga ctcccccaag
     1261 cggtgatcgc ccgggccaag gaagtgcttg caaacctgga aaaggccgag ttcaacgacc
     1321 tgggcgaacc cgtggcggcg ccgagaaccg gaaaggggga acgtgagaac gggggacgtg
     1381 gggaaaagac aggagagaca gcggtagagg gtaccgagcc gcaactcggg cttttcgcga
     1441 gcgaggcggg actcctgttc aaggagttgc aggacatgga tctggaccgt atgacgccgc
     1501 tcgaggccat gatcaagctg caggagctca agaagagggt ggaggggagc tgaaacaagt
     1561 ccagagttca aagtccagag ttcggagaac aaagcaaatc gcacgatccg cagagcccgc
     1621 accctgcaat tcgcaacccg aaaccgccat cctcgaattt ctcattgaca ctttgccccc
     1681 ctttctggta ctatcctgcc acaattatct actggaaagg agcagtctct gcaatgcagg
     1741 tcatattacg ggaagatatc gaaaaattgg gcaagatcgg cgacatggtg aaggtggccg
     1801 acggctatgc caggaactac ctcgtaccga caaagaaagc catcgaggcg acgccgaaga
     1861 acgtccacgc gatggagcac gcgaagaaaa tggtcgccga ccgtctccgc aagctgaaga
     1921 aggaagcggc ggccgatgcg gacaatatca aggcgcttgc ggtcacgatc aaggccaagg
     1981 ccggcgaaga gggcaagctg ttcggttccg tgacgaccat ggacatcgcc gaggccgcga
     2041 aggcccaggg tgtagtcatc gacaagagaa agatcgcgct cgaggagccg atcaagcggc
     2101 tcggcgacta taccgtcacc gtcaagcttc atgccgacat caccgctgag ttcaaggtgt
     2161 cggtcatcgc ggaagagagc gcttaaaagg cagtcgccgg gagccaggag ccaggagcca
     2221 ggagacagga tttttattct gacttctgac tcctgacttc tggcttcttt ttttccgaat
     2281 cccgaggttc ccccatgtcc gtcgagacag aagtctccct gcagaagctt ccccctcaga
     2341 acatcgaggc cgagcagatg gtgctcggcg ccgtcctgat cgagaatgac agcatcaaca
     2401 acgtcgcagc catcctctcc cccgacgatt tttataaaga tattcaccgg cgcatcttct
     2461 ccgtcatgct cgagatgttc gagcgccggg aggcaatcga cctagtgacg ctcacggact
     2521 gcctccgcgg caaggtggcg ctcgaatcgg tcggcggcgc atcctatctg gcgagcctgg
     2581 tgagcctcgt gcccacggct gccaacatca agtatcacgc gaagatcatc agggaaaagt
     2641 ccgttcttcg caggctgatc cacgcctcga ccgacatcat cacgcaatgc tacgacgatt
     2701 cacggaccgt ggacagcatc gacgaactgc tgaacactgc cgaacgctcc atct
//
LOCUS       DASSLD010000130         3022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1259640, whole genome shotgun sequence.
ACCESSION   DASSLD010000130 DASSLD010000000
VERSION     DASSLD010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259640"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFK23_02460"
     CDS             <1..190
                     /locus_tag="VFK23_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:1990904 - ribonucleoprotein complex
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET7317977.1"
                     /translation="ANKVQVKKAVEGIFKVKVADVTTVKNAGKKKRVGTRLGRRSDWK
                     KAYVTLKPGEKIDIFEGA"
     gene            191..1018
                     /gene="rplB"
                     /locus_tag="VFK23_02465"
     CDS             191..1018
                     /gene="rplB"
                     /locus_tag="VFK23_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET7317978.1"
                     /translation="MPVKSYNPTSPGRRFQTTSTFEEVTRSTPEKSLVVKISQSGGRN
                     NRGKVTVRYRGGWHKKKYRVIDFRRDKVGVPAKVAEIEYDPNRSARIALLHYADGEKR
                     YIIAPLSLKVGDAVLSGPQADIKPGNALPMTAIPVGTIIHNVELKKGAGGQLARSAGA
                     SVQLLAREGEYATLRLNSGEVRTVRSECMATIGQVGNLDHENISIGKAGRARWMGMRP
                     RVRGVAKNPVDHPLGGGEGKSSGGRPGCSPWGLPDGTKTRHNKVTSKYIITRRTKSR"
     gene            1088..1378
                     /gene="rpsS"
                     /locus_tag="VFK23_02470"
     CDS             1088..1378
                     /gene="rpsS"
                     /locus_tag="VFK23_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET7317979.1"
                     /translation="MPRSVKKGPYIEGSLMKKVEAMNTTGEKKIVKTWSRRSTITPEF
                     VGHTMAVHNGKKFIPVYVTENMVGHKLGEFSPTRTFKAHGGSKAAEKTTAPK"
     gene            1418..1747
                     /gene="rplV"
                     /locus_tag="VFK23_02475"
     CDS             1418..1747
                     /gene="rplV"
                     /locus_tag="VFK23_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET7317980.1"
                     /translation="MEASATIRHIRITPRKARQVIDLIRGKMVGDALNILTFTPLHAA
                     RVVKKLLDSAVANAEQKKVGDLDTLKVTKAFVNQGVTMKRMRARAMGRGNVIRKRTSH
                     ITLVLSA"
     gene            1767..2411
                     /gene="rpsC"
                     /locus_tag="VFK23_02480"
     CDS             1767..2411
                     /gene="rpsC"
                     /locus_tag="VFK23_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET7317981.1"
                     /translation="MGQKVHPIGLRLGIIKTWDSRWFSHKNYAALLHEDIKIRKIVKE
                     RLMHAGVSKVEIERAGQKAKINIHTARPGIIIGKKGAEVDKLKKDLEAMTGKQMYINI
                     QEIRRPELDAQLVAENIALQLERRIAFRRAMKKAVTSALRLGAQGIKITCAGRLAGAE
                     IARTEWYREGRVPLHTLRADINYGFFEAKTTMGQIGVKVWIYRGDVLQRPTTAA"
     gene            2457..2873
                     /gene="rplP"
                     /locus_tag="VFK23_02485"
     CDS             2457..2873
                     /gene="rplP"
                     /locus_tag="VFK23_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET7317982.1"
                     /translation="MLAPKRVKHRKMHKGRMKGTAYRGAEVSYGEYGLKVLEPGWISS
                     RQIEAARIAMTRYVKRGGKIWIRIFPDKPITKKPAETRMGKGKGAPEYWVAVVKPGRV
                     LYEMGGVDESVAREAFRLAAYKLPIATKFVSREAEI"
     gene            2870..>3022
                     /gene="rpmC"
                     /locus_tag="VFK23_02490"
     CDS             2870..>3022
                     /gene="rpmC"
                     /locus_tag="VFK23_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET7317983.1"
                     /translation="MKTRARELRDLSNEELQAKTADLKKELFNLRVQQAMGQIENPMR
                     LRSLRKD"
BASE COUNT          805 a          765 c          946 g          506 t
ORIGIN      
        1 tgccaacaag gtgcaggtga aaaaggccgt cgaagggatt ttcaaggtca aggtcgccga
       61 tgtaactacg gtaaagaatg caggcaagaa aaagcgggtc gggacgcggt tgggtcggag
      121 atccgactgg aagaaagcgt atgtgacgct gaagcctggt gaaaagatcg atatctttga
      181 gggtgcgtaa atgccggtaa agtcctataa tccaacgtct ccgggaagac ggttccagac
      241 cacctcgacg ttcgaagagg tgacgcgcag cacgcctgag aaatctctgg tcgtgaagat
      301 aagccagagc ggcggaagaa acaaccgcgg aaaggtaacg gttcgttacc ggggcggctg
      361 gcacaagaaa aaatacaggg tgatcgactt caggcgcgat aaagtcgggg ttcccgcgaa
      421 ggtagccgag atagaatatg atccgaatag atcggcccgt atcgcgctcc tccattacgc
      481 tgatggagag aagcgctata tcatcgcgcc gctcagtctc aaggtgggcg atgccgtgct
      541 ctccggccct caggccgaca tcaagccggg gaatgcgctc ccgatgaccg caatccccgt
      601 ggggaccatc atccacaacg tggagctcaa gaagggagca ggcggccagc tggcgcgaag
      661 cgccggggct tctgtgcagc ttctggcgcg tgaaggtgaa tatgccacgc ttcgtctgaa
      721 ctcgggggaa gtacgcaccg tccgctccga gtgcatggca accatcggtc aagtcggtaa
      781 cctcgatcac gagaacatca gcatcggcaa ggccggcagg gcgcgctgga tgggcatgcg
      841 gccgcgggtg cgtggtgtgg cgaagaaccc cgttgatcac ccccttggcg gaggggaagg
      901 aaaatcatcg ggcggcaggc ccggctgttc accctggggc cttcctgacg gaacaaagac
      961 gagacataat aaagtcacca gcaaatatat cataacgaga agaaccaaga gcaggtaacg
     1021 acacagcgac gaaggacgag acgaaaaagc gtccgacgtc cctcgttagt gtacaacgga
     1081 ggatacggtg ccgagatcag ttaaaaaagg cccttatatc gaaggaagcc tcatgaaaaa
     1141 agtagaggcc atgaatacga ccggagaaaa aaagattgta aagacttggt cccggaggtc
     1201 cacgataaca cctgaattcg ttggccacac catggcggtg cataatggca agaagttcat
     1261 tcccgtctat gtcaccgaga acatggtcgg acacaagttg ggggaatttt cgccgacccg
     1321 gacgttcaag gcgcacggtg gttcaaaggc cgcggaaaag acaactgctc cgaaatagaa
     1381 gcgggatctc gttgttcatg aaaaagagga tggtcagatg gaagcaagcg caacgataag
     1441 acatatacgc ataacaccgc gcaaagcgag acaggttatt gacctcattc gggggaagat
     1501 ggtgggcgat gccttgaata ttctcacctt tacgccgctc catgccgcgc gggtagtcaa
     1561 gaaacttctc gattcggccg tggccaatgc cgaacagaaa aaagtgggtg acctggatac
     1621 gctcaaggtg accaaggctt tcgtgaacca gggggtcacc atgaagcgga tgcgggccag
     1681 ggccatgggc cgcggcaatg taatcaggaa gaggaccagc cacataacgc tggtgctttc
     1741 agcgtaggaa taaaaacgga ggcatcatgg ggcagaaggt acatccgatc gggcttcggc
     1801 tcggaatcat taaaacgtgg gattcccgct ggttctccca caagaattac gcggcgttgc
     1861 tgcatgagga catcaagatc cgcaagatcg tgaaagagcg gctcatgcac gcgggcgttt
     1921 cgaaggtaga aatcgaacgg gccggtcaga aggccaagat caacattcac actgctcgcc
     1981 ccggtatcat catcggcaag aagggcgccg aggtcgacaa actgaagaaa gaccttgagg
     2041 ccatgaccgg caagcagatg tacatcaata tccaggagat ccgccgtccg gagctggatg
     2101 ctcaacttgt ggcggagaat atcgcactgc agctggagcg acgcatcgct ttccgcaggg
     2161 caatgaagaa ggccgtcact tcggccctcc gcctcggtgc acagggaatc aagataacct
     2221 gtgccgggcg cctggcggga gcggagatcg cgcgcaccga gtggtaccgc gaaggccgtg
     2281 ttccgctgca caccctgaga gcagacataa actacggatt ctttgaagcc aagaccacga
     2341 tgggccagat cggggtcaag gtctggattt accgcggcga tgtcctccag cggccgacca
     2401 cggcggcata accgattaca ccggaacacg ttccgagcga ttaaaggact gtcatcatgt
     2461 tagcacccaa acgagtcaaa cacaggaaga tgcataaggg aagaatgaag ggcactgcct
     2521 accgcggtgc agaggtgagc tacggcgagt acggcctcaa agtgctggag ccgggctgga
     2581 tctcaagcag gcagatcgag gcggcgcgta ttgccatgac ccgttatgtg aagcgcggcg
     2641 gcaagatctg gatcagaatt ttcccggaca agcctattac gaagaagccg gccgagaccc
     2701 gcatgggaaa aggcaaggga gcgccggagt actgggttgc ggttgtaaag cccggtcgcg
     2761 tgctctacga aatgggcggg gtcgacgagt ccgtcgcaag agaggcgttc cggctggcag
     2821 cgtacaaact gccgatcgcg acgaagttcg tttcccggga ggctgaaata tgaagacacg
     2881 agcacgggaa ctgagggacc tgtcgaatga ggaactgcag gcaaagacgg ccgacctcaa
     2941 gaaggagttg ttcaacctcc gggtccagca ggcgatgggc cagattgaaa atccgatgcg
     3001 cctccggtcg ctgcggaagg ac
//
LOCUS       DASSLD010000131         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1324417, whole genome shotgun sequence.
ACCESSION   DASSLD010000131 DASSLD010000000
VERSION     DASSLD010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324417"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFK23_02495"
     CDS             complement(<1..477)
                     /locus_tag="VFK23_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET7317984.1"
                     /translation="MSKLFSPMTLRGIEFKNRIFVSPMCQYSSPEGMPTDWHLVHLGS
                     RAVGGAGLVMVEATAVSREGRISPDDSGIWADRHAEAFAPIANFILEQRAVPGIQLAH
                     AGRKASTDAPWCGGRPLKPYERGWQPLAPSAIPFSDGFPIPREMTALDIEAVLGQFI"
     gene            complement(605..970)
                     /locus_tag="VFK23_02500"
     CDS             complement(605..970)
                     /locus_tag="VFK23_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317985.1"
                     /translation="MVRRTNRKVGIFAAIAALLLCLSFANVVVAIENLEGTPESIRGT
                     VIAIDTSLPMNILTLQSSEPGLSSPNKEITLFVDEQTVVTVCDTKTSLNDIRPGQEVQ
                     VSYFKLADLAFANSVYKGC"
     gene            complement(1146..>2065)
                     /locus_tag="VFK23_02505"
     CDS             complement(1146..>2065)
                     /locus_tag="VFK23_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019026955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7317986.1"
                     /translation="GLPEGIFSLVIGRNDVIGEAMLSDPRLPLISFTGSVPVGRHASE
                     VVAKRLGRTILELSGNNAIILTEHTDLNLALRAVVFGAVGTAGQRCTTTRRLIVHAAV
                     YDRVKDALIKAYEGLKIGSPLDEKNHVGPLIDKKAVDSFTDALKTGKEQGCRVVYGGE
                     VLSGAGYESGCYVVPAIVEAENHFPIVQKETFGPILYLIKYSGEVSRAIALHNGVTQG
                     LSSAVFTSDMREAETFLSPEGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDA
                     WKAYMRRQTVTINYGAELPLAQGIRFEVG"
BASE COUNT          432 a          622 c          553 g          458 t
ORIGIN      
        1 gatgaactgg ccaaggaccg cctcgatgtc cagtgccgtc atttcacggg gtatgggaaa
       61 gccgtcgctg aagggaatgg cgctgggagc gagcggctgc caaccgcgct catagggctt
      121 gagcggcctc ccgccgcacc acggcgcgtc ggtggatgcc ttgcgcccgg cgtgggcgag
      181 ctggattccg ggaaccgctc tctgttcgag gatgaagttg gcaatcgggg caaatgcctc
      241 ggcgtgcctg tccgcccaga tgccgctgtc atccgggctg atccggcctt cgcgacttac
      301 cgcggtcgcc tccaccatga caagacctgc accgcccacg gcacggctcc ccaggtgcac
      361 caaatgccag tccgttggca tcccctccgg gctggaatac tggcacatgg gagagacgaa
      421 gatgcggttt ttgaattcga tcccgcgcag ggtcataggt gaaaacagct tactcatttt
      481 gctcccgcct tcaatgatgt aaagtgaaag gatatatctc tgtactctgt actcactccc
      541 caaacctgat ccgtcaaaaa aacgccgcta cgtgcggcgt ttttccatgt tccctcattc
      601 agccctagca gcctttgtaa acggaatttg caaaggccaa gtccgccaat ttgaaatacg
      661 acacctggac ctcctggcct ggcctgatgt cgtttaatga cgtcttcgtg tcgcacaccg
      721 tcactacggt ctgctcatcc acgaaaagag tgatctcctt gttcggagac gacaggccgg
      781 gttcgctcga ctgcagagtc agaatattca tggggaggga cgtgtcaatg gcgataacag
      841 tgcccctgat actctccggc gttccttcca ggttttcaat cgccaccaca acattcgcaa
      901 aggacaggca aagaagtaag gcagctatgg ccgcaaaaat tcccactttc cggtttgttc
      961 gccgtaccat aatacgcgcc tcctttcttg cagaatttct tctttaattt aaatatattc
     1021 ccggttgcga gatttggcaa cagggggcta agcaaaaagg attattaacc gcgaatcgac
     1081 gcgaattaat gatttgcgtt cattcgcggt tccatactct gttgacacgg tcaaatatga
     1141 aatccttacc cgacctcaaa cctgatcccc tgcgccaaag gcagttctgc cccgtaattg
     1201 atggtaacgg tctggcgtcg catgtatgcc ttccaggcgt cggagccaga ttcccggccc
     1261 cctcctgtct ccttctcgcc gccgaaggcc ccgcctatct cggcccccga cgtgccgatg
     1321 ttgacgttgg caatcccaca gtcggacccc tcaggcgaca gaaacgtttc cgcctctctc
     1381 atatccgaag taaacacggc tgacgaaagc ccttgcgtaa cgccgttgtg gagggcgatg
     1441 gcccgggaaa cctctcctga atatttgatg agatagagta tcggtccgaa ggtctccttc
     1501 tgcacgatgg ggaagtggtt ctcggcctcc acaatagcgg gaaccacgta acagcctgac
     1561 tcataccctg ccccggagag cacctcccct ccgtatacaa cacggcaccc ctgttctttt
     1621 cccgttttaa gcgcgtcggt aaaagagtcc acagctttct tgtcgatcag gggtcccacg
     1681 tggtttttct catccagggg acttccgatc ttcaggcctt cgtaagcctt tatcagtgcg
     1741 tctttgaccc tgtcatacac ggccgcatgg acgatcagcc tgcgggtcgt tgtgcagcgc
     1801 tgtcctgccg tacccactgc cccgaagacc acggcccgaa gggcgaggtt aaggtcggtg
     1861 tgctcggtca ggatgatggc attattgccg gaaagctcga ggatggtcct tccgagccgt
     1921 ttcgccacga cctccgaagc atggcggccc acgggcaccg atccggtaaa ggagatgagc
     1981 ggcagacgcg gatcggatag catggcctcg ccgatgacat cgttccggcc gatcacgagg
     2041 gagaagatcc cctcggggag gccgt
//
LOCUS       DASSLD010000132         3821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_867684,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000132 DASSLD010000000
VERSION     DASSLD010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867684"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..476
                     /locus_tag="VFK23_02510"
     CDS             108..476
                     /locus_tag="VFK23_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7317987.1"
                     /translation="MLDFNSILVFSKDPKKLADFYKQVFQKDPDWSESGYYGFKAGNG
                     FITFGPHDKVKGRNANPERIMFNFETKDVKGEFERIKKLGAKAVAEPYNPSEDPKAMI
                     ATFADPDNNYFQLMTPWENK"
     gene            562..1830
                     /locus_tag="VFK23_02515"
     CDS             562..1830
                     /locus_tag="VFK23_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317988.1"
                     /translation="MNGFLRGTLLAALSGIAFALSGCGGSSNPSGAPAPTVVVQPLPL
                     PGPYAVACSNVVQDFSRVAVGEDVTSYWEGNPSDNGMTRYVTDLLADPGNTLIATVTA
                     PQDSNLFGSFAGQNVAFVVLACYPTTVDNPYDNYSLPTGKVVPHMQTGSDPPLFADNS
                     ARYPVVAFSHGYSGSPLSSDYISALSVFASYGYVVIAPFHGDLRFSDLKIDNLSDAIA
                     VASHLNNFTALQALRPLSISAALTLVLEHPQWQGHIDATQIGGFGASMGGETMMLLGG
                     AGLTTSYFDIGSSWKQVTHDPRIKAAVGYVPYFGQPVLPAFGRDQHGLDGINLTFLGI
                     SGTADTTAPILETEIGMSRLTGTRELVALAGVTHGFDVASTNDILTWTLTFLDAEVRG
                     NLAIQKKLSTMASVAGGGDDSVVIFYNGPP"
     gene            complement(1986..2579)
                     /locus_tag="VFK23_02520"
     CDS             complement(1986..2579)
                     /locus_tag="VFK23_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET7317989.1"
                     /translation="MIRSFVLADFITLANAGFGMAAIFLCLNHVTDSRANYTWAVFIL
                     LPLALVCDVLDGFVAHRLKRHSLLGADLDSLADIVSFGVAPAVLGFALGLQGMWDALI
                     LIFFVSCGISRLARFNATAEALSDGTGKVKYYEGTPIPSSIIIVIVLGIAFWQGRIDH
                     LLPFGSIRIGPWIFHPFTLLYAASGSAMISTFRIPKP"
     gene            3125..3565
                     /locus_tag="VFK23_02525"
     CDS             3125..3565
                     /locus_tag="VFK23_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1134 domain-containing protein"
                     /protein_id="HET7317990.1"
                     /translation="MKRFSAMVVVLGMVVTLAGLAFAAEREKTPDATLKLSEGQVALG
                     IGWSWGKGVLTMNGKDYPFKVGGLSVLDVGVTKAEAEGKVYNLKKIEDFNGTYVSAAA
                     EATVGVGVGATAMKNEKGVVIHLRPKTKGVNLKLAGEGVKFTLE"
BASE COUNT          886 a          993 c         1153 g          789 t
ORIGIN      
        1 tgcagcataa cgggattccg tcggaaataa tgagcgattg cgaggctggc tggagtggat
       61 ccttcgataa actggcaggg atcctctaat ctaaggaggt gaagattatg ctggatttca
      121 attcaatcct ggttttttcc aaggatccca aaaaactagc ggatttttac aagcaggttt
      181 ttcagaaaga tcccgattgg tcggaaagcg gttactacgg attcaaggct ggcaacggat
      241 tcatcacctt tggcccgcat gacaaagtca aaggtaggaa cgcgaacccc gaaaggataa
      301 tgttcaattt tgagaccaag gatgtaaagg gagaattcga aagaatcaaa aagctcgggg
      361 ccaaggcagt cgcggagcct tacaatccgt ccgaagatcc gaaagcgatg atcgcgacct
      421 tcgccgatcc ggacaataat tatttccagc tcatgacgcc ctgggagaat aaatgaacgg
      481 tcctgactgg agatggaaaa ttttgacaag cagcatggcc ggcatttttc ccgaaggggt
      541 tgccgtcgag gagattctgt catgaacgga ttcttgcggg gaacgctgtt ggcggccttg
      601 tcggggatcg cattcgcact ctccggatgt ggtggcagca gcaatccgtc aggcgccccg
      661 gcgccgaccg tcgtcgtgca gcctttgccg ttgccgggtc cctatgcggt tgcgtgcagc
      721 aacgttgtcc aggatttcag tcgagtggct gtcggcgagg acgtgacgag ctattgggaa
      781 ggaaatccgt cggacaacgg catgacgcgt tatgtgaccg atctgctggc ggacccgggg
      841 aatacgctga tcgccacggt gaccgcgccg caggactcga atctcttcgg ttcgtttgcc
      901 ggacagaatg tcgcgttcgt ggtgctcgca tgctatccca ctacggtcga caacccctat
      961 gacaactatt cgcttccgac cggcaaggtc gtgccacaca tgcagaccgg atcggatccg
     1021 ccgttatttg cggacaactc ggctcgttat cccgtggttg cgttttcgca tggctacagc
     1081 ggcagcccgc tctccagcga ctacatttcg gcgctgtccg ttttcgctag ttatggctac
     1141 gttgtcatcg ccccgtttca cggcgacctg cgcttttccg acctcaagat cgacaacctc
     1201 agcgacgcca ttgccgtggc gtcgcatttg aacaacttca cggcgctgca ggcactgcgg
     1261 ccgctttcga tttccgcggc gctcaccctc gtgctcgagc atccgcaatg gcaaggtcac
     1321 atcgatgcga cacagatcgg cggtttcggg gcgagcatgg gcggagagac gatgatgctg
     1381 ttgggcggcg ccggcctgac gacctcatac ttcgacatcg ggtcgtcgtg gaaacaggtc
     1441 acccacgatc cgcggatcaa ggcggcggtc ggctacgtgc cgtatttcgg gcagccggtt
     1501 ctgccggcat tcggacgcga ccagcacggg ctcgacggca tcaatctgac gttcctcggc
     1561 atcagcggga cggcggacac gaccgctccg atcctcgaaa cggagatcgg catgtcgcgc
     1621 ctcaccggga cccgcgagct ggttgcactc gcaggcgtga cccacgggtt cgacgttgcc
     1681 tcgaccaacg acatcttaac gtggacgctt acgtttctcg acgcggaggt tcgcggcaac
     1741 cttgcaatac aaaagaagct ctcgacgatg gcgagcgtgg cgggcggagg cgacgacagc
     1801 gttgtgatct tctacaacgg cccgccatag atgtgacgaa gacttgacgg cgaattgatg
     1861 ccaagtgacc gccctctcaa gcatttgcgg gggagcgaag aggggtatgc tttcccggaa
     1921 aagatccctt cgtgattttc gttgccggat gatcctcggt ttctgaggcc cgtgccttga
     1981 ttatctcaag gcttggggat gcggaaggtg ctgatcatcg cacttccgct cgccgcataa
     2041 aggagtgtaa aagggtggaa tatccatggc cctatccgaa tggatccaaa ggggaggaga
     2101 tggtcgatcc tgccctgcca aaatgcgatg ccaagaacga ttacaatgat gatactggag
     2161 gggatgggag tcccttcgta atatttcacc tttccggtcc cgtctgaaag cgcctccgcg
     2221 gtcgcattaa atcttgcaag ccggctgatt ccgcaactca cgaaaaagat taaaatcagg
     2281 gcatcccaca tgccttgcag tcccaatgca aaccccagga cagcgggcgc aacgccgaag
     2341 gaaacaatat cggccagtga atcgagatcg gccccgagaa gcgaatgccg ctttagacgg
     2401 tgagccacga acccatcgag aacgtcacag acaagcgcga gagggaggag gataaaaaca
     2461 gcccaggtat aattggcacg ggaatcggta acatgattca gacacagaaa gatagcggcc
     2521 ataccaaagc ctgcgtttgc caaggtgata aagtccgcca ggacaaatga tcggatcatc
     2581 gccatcgatt tttgggatgg gtccttcaaa ggtttattgg ctcctttctg taaggacgga
     2641 atttggtcat ccgattcaat ttcaatctgc ttttggatcc ccttaaggct tcattttatc
     2701 gaaaatgtat caatccgcac cgattaagtc cggcaaggaa ccgctttagg gggcatccct
     2761 gggggggatc tggaccgttt ggtcgcggaa ggttgccatg gagacgcccg aaactttcgc
     2821 gcatgagatt gaacctaaat taattggtag atccattccg attttgccaa ggaggtttct
     2881 atgtgccaga cctgtggatg tgaaccatgc aagacttgcg gtatggcgat caagaacggg
     2941 gtatgcgtcg gctgcggcaa gccatcggaa aaatgcactt gcaagaagaa ataggggagt
     3001 tcccgaaggc ataaacatca gataggcccc ggtgcgcgag atacgcagtt tgccggtgaa
     3061 cggggagtga cccgtgaact catcagggaa ggaggagaac atgaatatta tgaggagatt
     3121 caagatgaaa cgtttttcgg cgatggttgt ggtgcttggg atggtcgtga ccctggcagg
     3181 gctggctttc gcggcagaaa gggaaaagac gcccgacgcg acgctcaagc tttcggaagg
     3241 gcaggttgcc ttaggcatcg gatggagctg gggaaagggt gtcctgacaa tgaatgggaa
     3301 agactaccca ttcaaagtcg gagggctctc ggtactcgac gtgggagtca cgaaggccga
     3361 ggcggaaggc aaggtctata acctgaagaa gatcgaggac ttcaatggga cgtacgtgtc
     3421 cgccgccgcc gaagccactg tgggcgtcgg agtcggcgcc acggcgatga agaacgagaa
     3481 gggggtggtg atccatctcc gccccaaaac caaaggggtg aacctgaagt tggcagggga
     3541 aggcgtgaag ttcaccctcg agtagaatta gcggctggct caacggggtc ggggcactgc
     3601 cccggccccc cgaccgcctc ggtcgactaa aaaccggtcg gtggccccgg ggagtcagga
     3661 tcgcgggtat tggggccgag ggtcatgagt cccgcacgct catcgtggag gccacaatgg
     3721 aataccgacg actcggcaac accggtctca gcgtctctag aatctgcctc ggttgtatga
     3781 gctacggcga tcccgccgca atgatcgcgg gatcgactct g
//
LOCUS       DASSLD010000133         2331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_443934,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000133 DASSLD010000000
VERSION     DASSLD010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443934"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VFK23_02530"
     CDS             <1..511
                     /locus_tag="VFK23_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HET7317991.1"
                     /translation="AYLDEELEHRTLSEVREHLVEKMQEEKAIFAQLLDETYRASREV
                     QEKESEKVFIGGASQIMETPEFATVEKMRALFKAFEDKYMLLKLLDRTAAAQGIKVFI
                     GSENPYFEMQGCSLVVGSYHAGENIVGTLGVIGPTRMQYKQVIHVVDYTSKLLTKLLA
                     ERFQRGLEK"
     gene            508..1119
                     /gene="grpE"
                     /locus_tag="VFK23_02535"
     CDS             508..1119
                     /gene="grpE"
                     /locus_tag="VFK23_02535"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET7317992.1"
                     /translation="MRIDPEDMREDDGETAPSETADKEASETIEKLTQAVEQQTKAAA
                     EANEKYLRTYADFDNYRKRMQRDLADFRKYANEQLALELLSVVDHLGLALKHAAEAGE
                     ANDGLRQGVDMVYKQFRDILEKFGVKPFKAEGECFDPSKHDAVMQVTTAEAPENSVVQ
                     VFQEGYMYYDKVLRHAKVGVAKRPAGAPVGAEEKNAACDQKDS"
     gene            1165..>2331
                     /gene="dnaK"
                     /locus_tag="VFK23_02540"
     CDS             1165..>2331
                     /gene="dnaK"
                     /locus_tag="VFK23_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET7317993.1"
                     /translation="MAKVIGIDLGTTNSCVAIMEGGEPVVIANSEGSRTTPSVVAITD
                     SGERLVGQIAKRQAITNPDNTIFSIKRLIGRKYDTAEVQEARKHLPYKIVKADNGDAH
                     VEIRGKAYSPAEISAMILQKMKQTAEDYLGEKVTDAVITVPAYFNDSQRQATKDAGRI
                     AGLNVLRIINEPTAASLAYGLDKKKDETIAVYDLGGGTFDISILELGEGVFEVKSTNG
                     DTFLGGDDFDDRVIKWLAESFLKDSGIDLKKDKMALQRLKEAAEKAKIELSTVMETEI
                     NLPFITADASGPKHLLYKLSRSKYEQLVDDLVQKSIDPVRKALSDAGKTVDQINEAVL
                     VGGMTRMPKVISTVKDFFKKEPHRGVNPDEVVAIGAGIQGAVLTGQVKDVLLLDV"
BASE COUNT          575 a          626 c          742 g          388 t
ORIGIN      
        1 cgcctatctc gacgaggaac tcgaacaccg gaccctgagc gaggtcaggg agcatctggt
       61 cgaaaagatg caggaggaga aggcgatctt cgcgcagctc ctggacgaga cctaccgggc
      121 cagcagggaa gtgcaggaga aggaaagtga aaaggtcttc atcggcggcg cctcgcagat
      181 catggagacg cccgaatttg cgaccgttga gaaaatgcgc gctctgttca aggccttcga
      241 ggacaagtac atgctgctga aacttctcga ccggaccgcg gctgcgcagg ggattaaggt
      301 cttcatcggg tcggaaaatc cctatttcga gatgcagggt tgcagtctcg tggtgggcag
      361 ttatcatgcg ggcgagaaca tcgtgggcac gctcggggtc atcggaccca ccaggatgca
      421 gtacaaacag gttattcacg tagtggatta tacgtcgaag ctgctgacga agcttctggc
      481 ggaacggttt caaaggggct tggagaaatg aggatcgatc ctgaggacat gagagaagat
      541 gacggggaga ctgctccttc ggagacggcg gacaaggaag catcggagac gattgaaaag
      601 ctgacgcagg ccgtcgagca gcagacgaag gccgcggcgg aggcgaatga aaagtacctg
      661 cgcacgtatg ccgatttcga taattaccga aagcgcatgc agcgcgacct cgccgacttc
      721 cgcaagtacg ccaacgagca gctggccctc gagctcctct ccgtcgtcga tcacctgggg
      781 ctcgcgctca agcacgccgc cgaagcgggc gaagcgaacg acgggctccg ccagggtgtg
      841 gatatggtct ataagcagtt ccgcgacatc ctggagaagt tcggtgtcaa gccgttcaag
      901 gcggagggcg aatgctttga cccgtcgaaa cacgacgccg tcatgcaggt gaccaccgcg
      961 gaggccccgg aaaactcggt ggtgcaggtg ttccaggagg ggtatatgta ttacgacaag
     1021 gtccttcgcc atgcgaaggt cggcgtcgca aagaggccgg caggggcgcc agtgggagcg
     1081 gaagaaaaaa acgcggcgtg cgatcagaag gattcatagg aagcaccggc tcaccggtcg
     1141 atcaacatct tgaaggaggt ttttatggca aaggtaatcg gaatagattt aggcacgaca
     1201 aactcatgtg tcgccatcat ggaaggcggc gagccggttg tgatcgccaa ctccgagggg
     1261 agcaggacga ctccttcggt cgtggccatc actgacagcg gtgaacggct cgttggccag
     1321 atcgccaagc ggcaggctat caccaacccg gacaatacaa tcttctcgat caagcgcctc
     1381 attgggcgga aatatgacac ggccgaggtg caggaagcga gaaagcacct gccgtacaag
     1441 atcgtgaagg cggacaacgg cgacgcccac gtcgagatac ggggaaaggc atacagcccg
     1501 gctgagatct cggcgatgat cctccagaaa atgaaacaga cagccgagga ttatctcggt
     1561 gaaaaagtga cggacgccgt catcaccgtt ccggcctact ttaacgacag ccagcgccag
     1621 gcgaccaagg atgcaggaag gatcgcgggc ctgaacgtac tccggatcat caacgagccc
     1681 accgccgcgt cgctcgccta cggcctcgac aagaagaagg acgagaccat cgcggtctac
     1741 gacctgggag gcggcacctt cgacatctcc atcctggaac tgggcgaggg cgttttcgag
     1801 gtgaagtcca ccaacggaga caccttcctg ggcggcgacg atttcgacga ccgcgtcatt
     1861 aagtggctcg ccgaatcgtt cctcaaggat tcgggcatcg acctgaagaa ggacaagatg
     1921 gcgcttcagc ggctcaagga agccgcggaa aaagccaaga tagagctttc cacggtcatg
     1981 gagacggaga tcaacctccc cttcatcacc gcggatgcaa gcgggcccaa acacctcctg
     2041 tacaaacttt cgcggtccaa atacgagcag cttgtcgacg acctggtgca aaagagtatc
     2101 gacccggtga ggaaggcgct ttccgacgcg ggtaagaccg tcgatcagat caacgaggcg
     2161 gtgctggtgg gcggcatgac gcgcatgcca aaggtgatct ccacggtgaa ggatttcttc
     2221 aagaaagagc ctcatcgcgg cgtgaacccc gacgaggttg tggcgatcgg cgccggcatc
     2281 cagggcgcgg tgcttaccgg ccaggtaaag gacgtcctgc ttctggacgt g
//
LOCUS       DASSLD010000134         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1063226, whole genome shotgun sequence.
ACCESSION   DASSLD010000134 DASSLD010000000
VERSION     DASSLD010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063226"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /gene="gltA"
                     /locus_tag="VFK23_02545"
     CDS             complement(<1..1328)
                     /gene="gltA"
                     /locus_tag="VFK23_02545"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HET7317994.1"
                     /translation="MTEEKKTPKKIVPKKTPIPEQPPQVRRSNFQEVSLGYTPEMAMQ
                     EAARCLTCKTPTCVEGCPVNVPIPRFIKAIKEGDFKKAISIIKEANLLPAVCGRVCPQ
                     ESQCEKNCLLGKKFDPVAIGRLERFAADQSLSNGGPQAPQVAPKTGRKIAVIGSGPSG
                     LTCAYELARQGHSVVIYEALHEAGGVLVYGIPEFRLPKKIVHREIEVLKSMGVEIVTN
                     AVAGKLFTIDEIMKEFDACFIGSGAGLPKFMNIDGENLNGVYSANEFLTRINLMRAYQ
                     FPEFDTPVMRGDRVAVFGGGNVAMDAARTALRIGAEEVILIYRRSLTELPARKEEVHH
                     AQEEGIRFELCTNPVRILGNDQAQVRAVECVRMDLCELDESGRRSPKPIKGSEFEIPV
                     NVAIVALGTGANPLIPQSAKNLQVNKRLYIIADEETGRTSIEGVYAGGDIV"
     gene            complement(1332..2183)
                     /locus_tag="VFK23_02550"
     CDS             complement(1332..2183)
                     /locus_tag="VFK23_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HET7317995.1"
                     /translation="MPRILEKKLLRKPDVFYFKVAAPLISKKAQPGQFVILRLHAKGE
                     RIPISLADINPDEGTISLIVMAVGKTTTEMSVMKAGDDILDLCGPLGNPTHIDHAGKV
                     IVVGGGFGVAPLFPIARAFKAAGNEVVCVMGARSKDLLIYEQEMAEVCSRVLVSTDDG
                     SKGMKGVVTEALRQELEKGGTGLVVTVGPAIMMKFVSDTTRPFGVKTMVSLNPIMVDG
                     TGMCGGCRVTVGGQTLFACTDGPEFDGHQVDWGVLMNRQKTYVPEQRESFESWKQRHA
                     GVLCERG"
     gene            2503..>2719
                     /locus_tag="VFK23_02555"
     CDS             2503..>2719
                     /locus_tag="VFK23_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET7317996.1"
                     /translation="MSILAMILAGGKGERLHPLTMHRAKPAVPFGGIYRIIDFTLSNC
                     INSGIRKIAVLPQYKSLSLDKHLRLAWN"
BASE COUNT          554 a          803 c          729 g          633 t
ORIGIN      
        1 acgatatcgc ccccggcata cacgccctcg atgcttgtcc tgcctgtttc ctcgtccgcg
       61 atgatataga gtctcttgtt gacctgaaga tttttcgcgc tctgggggat caggggattt
      121 gccccggttc cgagagcgac aatggcgaca tttaccggga tctcgaactc cgagcccttg
      181 atcggcttcg gggaccttct gccggactca tcgagttcgc acaggtccat cctgacgcac
      241 tcgactgccc gcacctgggc ctggtcgtta ccgagtatcc gcacggggtt cgtgcagagc
      301 tcgaaccgga tcccttcctc ctgggcgtga tggacctcct ccttgcgtgc gggcagctcg
      361 gtgagcgaac ggcggtagat gagaatgacc tcttcggcac cgatgcggag agcggtccgg
      421 gccgcatcca tggcgacgtt gccgccgccg aagacagcga cgcggtctcc gcgcatgacc
      481 ggcgtgtcga actccgggaa ctggtacgcc cgcatcaagt tgatccgggt caggaactcg
      541 ttggcggaat agactccgtt caggttctcg ccgtcgatgt tcatgaattt cggcaagccg
      601 gccccgctcc cgatgaagca ggcgtcgaac tccttcatga tttcgtcgat ggtgaagagc
      661 ttgcctgcca cggcgttggt cacgatctcg acgcccatgc tcttgagcac ctcgatctca
      721 cggtgtacga tctttttcgg cagacgaaac tcggggatgc cgtagaccag gacgcctccg
      781 gcctcgtgga gcgcctcgta gatgaccacg ctgtgaccct ggcgggccag ttcataggcg
      841 caggtgagac ccgacgggcc gctaccaatg acggctatct tcctgccggt cttcggggct
      901 acctgaggtg cctggggccc gccgttcgaa agagactgat cggcggcaaa acgctcgagc
      961 cggccgatag ccaccggatc gaacttcttc cccaaaagac agtttttctc acactggctc
     1021 tcctggggac agacccgtcc gcagaccgcg gggagcaggt ttgcctcctt gatgatgctg
     1081 atggcttttt tgaagtcacc ttccttgata gccttgatga accgggggat cggcacgttg
     1141 accgggcagc cttcaacgca tgtgggcgtc ttgcaggtga ggcaccgggc cgcctcctgc
     1201 atcgccatct ccggcgtgta cccgagggac acttcttgaa aattggagcg ccggacctgc
     1261 gggggctgtt ctgggatcgg ggtctttttc gggacgatct tttttggagt tttcttttct
     1321 tcagtcatcg ctcacccccg ttcgcagagc acgcctgcgt gccgctgctt ccatgattca
     1381 aaggattccc gctgttccgg gacataggtc ttctggcggt tcatcaggac gccccagtcc
     1441 acctggtgcc catcgaattc aggaccgtcg gtgcatgcga acagtgtctg gccgcccacg
     1501 gttacgcgac agcccccaca catccccgtt ccgtccacca tgatagggtt caggctcacc
     1561 atcgttttca caccgaaggg acgggtcgta tcagaaacga acttcatcat gatcgccggt
     1621 cccacagtga ccacaagacc ggtgccgccc ttctcaagct cctgccggag cgcctcggtc
     1681 accacccctt tcataccctt gcttccgtca tcggtggaaa cgaggacgcg gctgcaaacc
     1741 tctgccattt cctgctcata aatcaaaagg tccttggacc tggctcccat gacgcagacg
     1801 acctcgttgc ccgctgcctt gaaggctcgt gctatgggaa agagcggtgc tacgccaaag
     1861 cctcctccca cgacgatgac cttgccggca tgatctatat gtgtgggatt acctaggggg
     1921 ccgcagagat cgaggatatc atcgcctgcc ttcatgaccg acatctccgt ggtcgtcttg
     1981 cctacggcca tgacgatcag gctgatggtg ccctcgtcgg gattaatgtc cgccaaagag
     2041 atcgggatcc gctcgccctt tgcatgaagc cgcaggatga cgaattggcc gggctgcgcc
     2101 ttcttcgaga tgagcggggc cgcgaccttg aaataaaaaa catccggttt tctaagcaat
     2161 ttcttttcca ggattcttgg catgttcgaa tagctccttt tacaagctgt agagattgtg
     2221 gtcgtcgggg tctgtcgcca attgaaaaag tactttgagg gatagaacag acgtgacttg
     2281 ctacaatagg taacatttta ccccttaatt cgcaaaattg aaagaaaaaa aacgaaatta
     2341 gttaaacagt tagtaatatg cgaaccaata taaattcacg aaaaaaccgt aaataattct
     2401 aataattttg aacgtatgta catcatcctt gtttgacagc atcattagct cctgctattc
     2461 taaatagtgt ttaaaggtat atatattttt aggagacgaa atatgagcat cctcgcaatg
     2521 atccttgccg gcggcaaggg agaacgtctt catccgctta ccatgcaccg ggccaaacct
     2581 gcggtgccct ttggcggcat ctatcgcatc atcgatttta ccctctccaa ctgcatcaat
     2641 tcgggcatcc gcaagatcgc cgtcctgccg cagtacaagt cgctttcgct ggacaagcac
     2701 cttcgccttg cctggaacc
//
LOCUS       DASSLD010000135         5545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1618160, whole genome shotgun sequence.
ACCESSION   DASSLD010000135 DASSLD010000000
VERSION     DASSLD010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618160"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFK23_02560"
     CDS             complement(<1..342)
                     /locus_tag="VFK23_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7317997.1"
                     /translation="MSFLKKGFVVGLVVLGIVALLVGFSIRATVEAADVKVIEVKEVP
                     AAKIPTFDATAYIAGHGGHLAILDMKTMKAPTDVEKDRIVLSEAGSEMEGKIAGMSFE
                     EVKKSGGSHGQA"
     gene            complement(812..1006)
                     /gene="rpmB"
                     /locus_tag="VFK23_02565"
     CDS             complement(812..1006)
                     /gene="rpmB"
                     /locus_tag="VFK23_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET7317998.1"
                     /translation="MAGTCQICGKGRLSGNKVSHSNNKTKKMSKPNLQFVKAVGPHGS
                     HVRMHVCTRCIRSGKVKKVS"
     gene            1190..1759
                     /locus_tag="VFK23_02570"
     CDS             1190..1759
                     /locus_tag="VFK23_02570"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7317999.1"
                     /translation="MQATLKLISEKGYLGTTTREIANEAGVTEITLFRNFGSKELLFE
                     EVLKTYTFLPRLKELLPKLEGLSCEDALVLIATRFLQTLKERKELIQIMYQEVTSYPE
                     KIKDVYNKFLAEVRSTLANYITTLQERGELRKDVTPEMAARVFLAMFFAYFRQEEIMR
                     PGGMKKQNMEKYIRDVVDIYMHGTLDGRR"
     gene            1783..3177
                     /locus_tag="VFK23_02575"
     CDS             1783..3177
                     /locus_tag="VFK23_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7318000.1"
                     /translation="MQGYVGAKKWRLEAALYILLIGAVPFHPLFAAEPEGAVPLLPAP
                     GESETRDHPDEGAAAKSETLTLGRCIEIALEKNPTILASRYGVEVNASRVGEARSGYY
                     PQLSASAAYTRINPVPVAPATSSGTFNQFSSSITLNQNLLDFGKTSSQVDVSKYNLEA
                     SRSDLDSTQDTIILNVKQAYYGVLQARRNRDVAADVIKQFQLHLDQAKGFYDVGTKAR
                     IDVIKAEVDLSNAHLDLINKDNAFKIAWVTLKNVMGVPDAPEGTYSIEEKLTFEKYEI
                     TLQEASNRALEKRPDYRSAISKRQAAEMNIDLQKSGYYPVLSANADYSWAEVDLPPYQ
                     LNHGWSGGVVLTVPLFSGFLTSHQVAEAKSNLYVLKANEESLRQQIMFEVRQAYLNLQ
                     AAEASIATAQLAADQAKENLDLANGRYAAGVGSPIEVSDAFATYVTAQANYTSALSNY
                     KIAQASIVKAMGER"
     gene            3251..4429
                     /locus_tag="VFK23_02580"
     CDS             3251..4429
                     /locus_tag="VFK23_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7318001.1"
                     /translation="MDTIRMWRVSPSGFLAVMMLVVVFLAGCSDGKAKQVPQRVVPVK
                     IGDVTRQDVPVQINAIGNVEAYNTVSVKAQVGGEVIDVHFKEGQDVKQGDLLFQIDPR
                     PYEMAIKQAEAQLARDAAQAKNAEEQAKRYAILVQKDYVSKDQYDQLRANADALAAAV
                     LADKANVEGLKLQYSFCTIKSPIDGRVGSVLVNKGNIIKANDLVMVTINKIMPIYITF
                     SVPEQNLADIKKYEAEGSLRVEAVIPGDEKRPAKGVLTFINNSVDTSTGTIQLKGTFE
                     NRDKRLWPGQFVNVLLRLTTERNAVVMPSAALQTGQQGQYVFVVKSDFTVESRQVAVS
                     RSLGDLAVVSQGASPGEKVVIDGQLNLIPGARVELKGENGGNPKPGEGNVKSQITNSK
                     "
     gene            4532..>5545
                     /locus_tag="VFK23_02585"
     CDS             4532..>5545
                     /locus_tag="VFK23_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7318002.1"
                     /translation="MHISEPFIKRPVMTTLVMSAILLFGIMAYRMLPVSDLPNVDFPT
                     VLVNASLPGASPETMASAVATPLERQFSTIAGLDNMTSVSGQGVAQITLQFNLSRNID
                     AAAEDVSAMIAKSARLLPPSMPTPPSFQKVNPADQPIIYVALSSPTLPLSAVDEYAET
                     AMAQRISMISGVAQVQVVGSQKYAVRARLDPRALASRGIGIDEVANAVSRGNVNLPTG
                     TLQGATKSFTIEASGQLMDAAAYRPLIVAYRSGNPVRLQELGMVVDSVENDKVASWFN
                     TAKEKQRAVILAIQRQPGTNTVEVVDSIKALLPVFRAQLPASVNLDILFDRSVSIRES
                     VNDV"
BASE COUNT         1339 a         1495 c         1549 g         1162 t
ORIGIN      
        1 tgcctggccg tgggaaccgc cggatttctt gacctcttcg aagctcatcc cggcgatctt
       61 gccttccatc tccgatcctg cctcggagag aacgatacgg tctttctcaa catcggtcgg
      121 tgccttcatc gtcttcatgt cgagtatcgc caggtggccg ccgtggccgg cgatgtaggc
      181 cgtcgcatcg aatgtcggga tctttgccgc aggaacttct tttacctcga tgaccttgac
      241 atccgcagct tcaaccgttg cgcgaatgga aaagcccacg agcagggcca ctataccgag
      301 aactaccaga ccgactacga accctttttt cagaaaactc atagttcctc ccctttgaat
      361 aaagttgacc gccataacgt gcccggatga tgcattattc ctttttcccg atgaaaatac
      421 caatccagct gatgacgatt ttgaagtcca cctccttact ctgcaaagca gggaatatag
      481 ggtcagtact tgattccgaa cagtgtttta actcgataat gatagtatac tcatccctcc
      541 ggtgtcaact tcaaattcta actttatatt acttcttgcc tgcaatttag tgtgttcttt
      601 agcatttttt atgattgcgt taagccttta agcagcacgc gtgtcatagt catagtcagc
      661 tgtggacccg tccttgttcc aaaccggcag ggaccgcgag gacgttttga ctaataaaaa
      721 ccaggatgtg aaatggggga gggaggacaa aaaaaatccc cagccgtacg aacggaacgg
      781 cgggggatat cataaggccc gtgtgagact gtcaactgac cttttttacc ttgccggagc
      841 ggatgcagcg cgtgcatacg tgcatgcgta catgagaacc gtgaggtcct accgctttca
      901 cgaactgcaa gttcggtttt gacatttttt tggtcttgtt gttcgaatgg ctcaccttgt
      961 tgccgctgag ccggcctttc ccgcatatct gacatgtgcc tgccacagtg atatcctccg
     1021 ttaatgcgac aatatttgct gcaatgcagg cgctaacata ccataagaga acagtccgcg
     1081 caagggaaaa ttatgcccgc atgccaaaat ttcttgacat ccatcagcac ttcccgtatt
     1141 gtaagtacat actaacagta tgagaacgtc gaatacaaaa gaattattgt tgcaggccac
     1201 cctgaaactt atttccgaga aagggtatct cggcaccacg acgagagaaa ttgccaacga
     1261 ggccggcgtg accgagatca ctcttttcag aaatttcggg tccaaggaac tgttgtttga
     1321 agaggtcctg aagacctata ctttcctgcc gaggctgaag gaactgctcc cgaaactgga
     1381 aggcctttcc tgcgaggacg cgcttgtgct gattgcaacc cggttcctcc agacgctgaa
     1441 ggagcgtaag gaactgatcc agatcatgta tcaggaggtg accagttatc ccgagaaaat
     1501 caaggatgtc tacaataagt ttctcgcgga agtaaggtct acgcttgcga actatatcac
     1561 gacattgcag gagcggggag aactgaggaa ggatgtgact ccggaaatgg ctgcgagggt
     1621 ttttctggcg atgttctttg cctatttccg gcaggaagaa atcatgcggc cgggcgggat
     1681 gaagaagcaa aacatggaga agtacattcg ggacgtggtc gatatctata tgcacggcac
     1741 attggacggc agacgatagg cgtggatcga gggagagtcg gtatgcaggg atatgttgga
     1801 gcgaagaagt ggaggctgga ggcagccttg tacatcctgt tgatcggggc ggttccgttc
     1861 caccccctct ttgcggcaga gcccgagggg gccgtcccct tgctgccggc acccggagag
     1921 tccgaaacaa gggatcatcc cgatgaaggc gccgccgcga agagcgagac attgacactc
     1981 ggccggtgta tcgagatcgc gctcgagaag aacccgacca tccttgcatc gcgctacggg
     2041 gtcgaagtca acgcgagcag ggtgggcgaa gcgcgctccg gctattaccc gcagctcagc
     2101 gcctcggcgg cctacaccag gatcaatccc gtcccggtcg cgccggcgac ttcctcggga
     2161 accttcaacc agttttccag cagcataaca ctcaaccaga acctgctgga tttcggaaag
     2221 acttcgtctc aggtcgatgt gtcgaaatat aatctcgaag cttcccgctc ggaccttgat
     2281 tcgacgcagg atacgatcat cctgaacgtg aagcaggcct attacggcgt cctccaggcc
     2341 aggagaaacc gcgatgtcgc tgctgacgtc ataaaacagt ttcaactgca tctcgaccag
     2401 gcgaagggct tttacgacgt cggaacgaag gcgagaatag acgtcatcaa ggcggaagtg
     2461 gacctgagta acgcgcacct ggatctcatc aacaaggaca atgcctttaa aatagcctgg
     2521 gtcaccctaa agaatgtcat gggggtcccc gatgcgccgg aaggcaccta ctcgatcgag
     2581 gaaaagctca cttttgaaaa atacgagata accttgcaag aagcgtcaaa tcgcgcgctg
     2641 gagaagcgac ccgattacag gtcagccatc tcaaaaagac aggcggcgga gatgaatatc
     2701 gatcttcaga aatcggggta ttatcccgtt ttatcggcaa atgcggacta tagctgggcg
     2761 gaagtcgatc tgccgcccta tcagcttaat cacggttgga gcggcggcgt ggtcctgacc
     2821 gttcccctgt tcagcggttt tctgaccagc catcaggtgg ctgaggcaaa atcgaacctc
     2881 tatgtcctga aggcgaatga agagtctctg cgacagcaga tcatgttcga ggtgcggcag
     2941 gcctatctga acttgcaggc cgccgaggcc agcattgcca cggcccagct tgccgccgat
     3001 caggccaagg agaacctgga cctggcaaac ggcagatatg cagccggcgt gggaagcccc
     3061 atcgaggtct cagacgcctt tgccacgtat gtaacggccc aggcgaatta taccagcgcc
     3121 ctgtccaatt acaaaatagc tcaggcgagt atcgtgaagg ccatgggcga gagataaatt
     3181 aaagaatgcg gagtatgaaa tgcggaatgc ggatttaaag gatcttatca aaggcggaga
     3241 aacaaggata atggacacca taagaatgtg gcgggtctct ccatccggtt ttctggctgt
     3301 catgatgctg gtagtcgtat ttttggccgg ttgttcagac ggcaaggcga agcaggtacc
     3361 gcagcgagtg gtcccggtga aaatcgggga cgtgacccga caggacgttc ccgtgcagat
     3421 caacgccatc ggcaacgtcg aggcctacaa caccgtctcg gtgaaggctc aggtcggcgg
     3481 ggaagtgatc gacgtacatt tcaaggaagg ccaggacgtg aaacagggag acctgctctt
     3541 tcagatagat cctcgtccct atgagatggc cataaagcag gccgaggccc agctcgcgag
     3601 ggacgcggcc caggcaaaga acgctgaaga gcaggccaag agatacgcaa tcctggtcca
     3661 aaaagactat gtctccaaag accaatacga ccagctccgc gcgaacgccg acgctctggc
     3721 ggcagcggtc ctcgcggaca aggccaatgt agagggcttg aaacttcaat actctttctg
     3781 cacgatcaag tctcccatcg acggccgcgt cgggagcgtc ctggtcaaca aaggcaacat
     3841 tatcaaggcg aatgacctcg tgatggtgac aatcaataaa atcatgccta tctacattac
     3901 tttttccgtg cccgaacaga acctcgcgga catcaagaaa tacgaggcag agggatcact
     3961 acgggtggag gcggtcatcc cgggcgacga aaaaagaccc gccaagggag tgctcacgtt
     4021 cattaataac tccgtggata cttcaacagg gaccattcag ctcaagggca ctttcgagaa
     4081 cagggacaaa cggctctggc ccggccagtt tgtgaacgtg cttctcaggc tcactacaga
     4141 gcggaatgcc gtggtcatgc cgtccgctgc gctccagaca ggccagcagg gacagtatgt
     4201 gttcgtggta aaatcggact tcacggttga atcacgtcag gtggccgtct cgcgttcgtt
     4261 gggcgacctc gccgtggtct cccagggggc ctcacccgga gaaaaagtgg ttattgacgg
     4321 acaactgaac ctgattcccg gcgcacgggt tgaactaaaa ggtgaaaacg gggggaatcc
     4381 gaaaccgggc gaggggaatg ttaaatccca aataacaaat tccaaataag cgccagatgc
     4441 ccgatgataa atgacaattc ctttgacatg tcatcggtgg gaggcattta tggagttttg
     4501 gtgcctcaga tttggagctt gacataattc tatgcatata tcagagccct ttataaaaag
     4561 gccggtcatg acgacgctgg tcatgtcggc aattctgctc ttcggtatca tggcgtaccg
     4621 gatgctgccg gtcagcgacc ttccgaacgt ggatttcccg accgtactcg tgaacgccag
     4681 ccttcccggg gcgagcccgg agaccatggc ctctgccgtg gcgacgccgc tggagcgtca
     4741 gttctcgacc atcgcgggac tcgacaacat gacttccgtg agcgggcagg gcgtcgcgca
     4801 gatcaccctc cagttcaacc tgagcaggaa catcgacgcg gcggcggagg atgtgtccgc
     4861 catgatcgcc aaatctgcgc ggctgctccc gccatccatg cccacgcccc ccagcttcca
     4921 gaaggtcaat cccgccgacc agccgatcat ctacgtcgcg ctcagttctc ccacgctgcc
     4981 cctctccgcg gtcgatgaat acgcggagac cgcgatggcg cagcgcatct ccatgatcag
     5041 cggcgtggca caggtgcagg tggtcggctc gcagaagtac gcggttcgcg cccggcttga
     5101 tccccgtgca ctggcgtcgc gggggatagg gatcgatgag gtggcaaatg cggtttcccg
     5161 ggggaacgtg aacctgccga cggggaccct tcagggggca acgaagtcgt tcaccatcga
     5221 ggcaagcggc cagcttatgg atgcggccgc ctatcgccct ctcatcgtgg cctaccgcag
     5281 cggcaacccc gtgcggctcc aggaactcgg catggtagta gacagtgtgg agaacgacaa
     5341 ggttgcaagc tggttcaaca ccgcaaagga aaaacaacgg gcggtgatcc tggccatcca
     5401 gcgccagccc ggcacgaaca ccgtcgaggt ggtggattcc atcaaggcgc tcctgcctgt
     5461 ctttcgcgcg cagctgccgg cgtcggtgaa tcttgacatt ctcttcgacc ggtccgtgtc
     5521 cattcgggaa tcggtcaatg atgtc
//
LOCUS       DASSLD010000136         3958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1529963, whole genome shotgun sequence.
ACCESSION   DASSLD010000136 DASSLD010000000
VERSION     DASSLD010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529963"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="VFK23_02590"
     CDS             complement(<1..1204)
                     /locus_tag="VFK23_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET7318003.1"
                     /translation="MGKQAVTKHNSTDRVRRYFADLGPGLITGAADDDPSGISTYSVT
                     GASFGYMPLWTALFSFPLMAAVQLMCARLGMVTGRGLAGVVRRYYPRWVLWSACTLLI
                     IANVFNIGADLGGMAEATEMVTGIKSYYWTPLYSILIILLLFFSSYRQFARIFKWLTM
                     VLFAYIAAAVLARPDWHSVLNATFVPRVEWSSAFLATFVGILGTTISPYLFFWQASQE
                     VENERAQGKRTVKQREGATIKELRVARTDVFTGMFFSNLVMYFIILTTAATLHAHGQT
                     NIQTAREAAEALRPFAGSGAYLLFTIGIIGTGILAVPVLAGSAAYAVAEAAAWRGTLE
                     DPPRLSPRFYGLIAVSMLLGLALDFVGFNAVRMLFYSAVLNGVLAPPLIVLVAILTSK
                     SEIMGIRVS"
     gene            1897..2190
                     /locus_tag="VFK23_02595"
     CDS             1897..2190
                     /locus_tag="VFK23_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HET7318004.1"
                     /translation="MSTYIWLVNWTDQGIRNVKETTKRAKSFKENAEKIGVNVQEILW
                     TLGRYDLVLVIDAPNDETISRLALGVGMGGNAKTETLKAFSAQAMDQILKGFP"
     gene            2316..>3958
                     /gene="polX"
                     /locus_tag="VFK23_02600"
     CDS             2316..>3958
                     /gene="polX"
                     /locus_tag="VFK23_02600"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HET7318005.1"
                     /translation="MINPQVANVFHEIADLLELSGDNPFRIRAYRRAAMNVESIPKDV
                     AAMSEEELQALPGIGKDLAHKIRQFVDTGRIDAHEELQKKIPRGLLEILRVPGIGPKT
                     ARLLFDKKNVKSLEELETLARSGNLTGLPGIQAKTEENIRKGIGQLRKGMERFPLGKA
                     LPIARGILEHLQKAVPGARFALAGSIRRWKETVKDIDILAAGKMPKVITKEFVDLPGT
                     REVLERGATKSSLLTEGGIQVDLRVVAEESFGAALQYFTGSKEHNVRLREMASRRGLK
                     INEYGVFREADSRRLGGREEEDVYKALGLPFVPPELREDAGEIEAAIEGRLPDLVSLA
                     DIRGDLHVHTKWSDGAHDLDALVGAARARKYGYIAITDHSKGLGVAHGLTEDRVRKQM
                     GMIDAANRKLKGFRILKGIEVDIRGDGTLDLPDTVLSELDIVVASIHSGFRQPGEQLT
                     KRLVSAIRNPFVSIIAHPTGRLLGEREAYPVNMETVISAAAKHGKALEINAYPLRLDL
                     SDAWARVAMQRRVPLAVSTDTHILSNLDFMGYGVSIARRG"
BASE COUNT          950 a         1113 c         1141 g          754 t
ORIGIN      
        1 aactgactcg tatacccatt atttcggatt tgctcgtcag tatcgccacc aagacaatca
       61 atggcggtgc cagcacccca ttgagcaccg ccgaataaaa gagcattctc accgcgttga
      121 atcccacaaa atccaacgcc agacctaaca gcatcgaaac ggcgatcaat ccgtaaaacc
      181 ggggtgataa tcgtggtggg tcttccaatg ttccgcgcca cgccgccgct tccgccactg
      241 cataagcggc agagcctgcc aggacgggaa cagcaagtat ccccgtcccg attatgccaa
      301 ttgtgaataa caggtaagct ccacttccag cgaaaggacg caacgcttca gccgcttccc
      361 gcgccgtttg tatgttggtt tgcccatgcg cgtgcaatgt cgccgcggta gtcagaatga
      421 tgaaatacat tacgagatta gagaaaaaca tccccgtaaa aacatccgtt cgggccactc
      481 tcaattcctt gatggtcgcc ccttcacgct gtttcactgt ccgtttccct tgcgcccgtt
      541 cgttttcaac ttcttgagat gcttgccaga agaaaagata gggcgagatg gtcgttccga
      601 ggatgccaac aaaggtcgca agaaacgcac tcgaccattc gacccggggc acaaaggttg
      661 cgttcaggac agagtgccag tctggtctgg cgaggacggc tgccgcaata taggcgaaga
      721 gaaccatcgt caaccatttg aagatgcgag cgaattgacg gtatgatgaa aaaaagagca
      781 gtaagatgat cagtatggaa tacagtggag tccagtagta cgacttaatt cccgtcacca
      841 tttcggtcgc ttcagccatg cccccgaggt cggcaccgat gttgaaaacg ttcgcaataa
      901 tcaagagcgt acacgcagac cacaacaccc aacgcggata atatcgtcgc acgactcctg
      961 ccagcccgcg ccccgtgacc ataccaagtc gcgcacacat caactgtaca gcagccatca
     1021 gcgggaaaga gaagagcgcc gtccataagg gcatatagcc gaaggacgcc ccggtcaccg
     1081 aataagtaga tatgcctgaa gggtcgtcat cggcggcacc ggtaattaac ccgggaccaa
     1141 gatcggcaaa gtaccgtcgt actctgtccg tcgaattatg ctttgtcaca gcttgttttc
     1201 ccatcgtaga gttattttaa ttcccttcgt ttcattttcg tatgaattcg ataaccagga
     1261 gtacgtgaag gaatcctccc atcgcgtacg acgtgttttc atcaggctat tcctgccagc
     1321 cagccttcgg aatcggcttc tgtccgaaat acgagtcgta atttgtctga atctctggtg
     1381 ggtgtttgtc ctatagcatg gagaggcaga accatttgaa aaatatcata aaatagtgtt
     1441 tgattgtatg aaaaaaagag atcttatcca atgccaaatc aggcaagagg cattaaaata
     1501 attattggcc cagtaatatt tcccggaatt cgagtttcgg gcgggaggcg cagcagaaac
     1561 gggaaggccc ggctgtggcc gccgggcctt cgcagagcgc ggggaaattc ggcgtggcgt
     1621 tccggtcgtt cggtacctga cttccgactg caaccccact tccgtgcgac gacaagcaga
     1681 gccaatccca gccaagctaa tctttcaact tcacaccagc cgccgcgctt catcatatgt
     1741 ataacgtaat tttagatccc ttgtcaagtg gttccctccc gattttccca agacagcttt
     1801 tcccccgcat aaaacgaaaa atctccggtt tgtaaaacct catcttattt ttaatcttcg
     1861 ccttgaccca catcagacat ggaaaggaga ggaacgatgt cgacctacat ctggctggtc
     1921 aactggacgg accaagggat ccggaacgtg aaggaaacaa ccaaacgtgc gaaaagtttc
     1981 aaggagaatg cggaaaagat cggagtgaat gtccaggaga tcctttggac attgggtcga
     2041 tacgacctcg tgctggtgat cgatgccccc aatgacgaaa caataagccg tctggcgctg
     2101 ggagtgggca tgggggggaa tgccaagacg gagactctca aggccttttc ggctcaggcg
     2161 atggaccaga tcctcaaggg ttttccgtag aagatcagag gagcgatgtc agggagacag
     2221 atcatcaccg atcatcccgg aaatccactc gttgtatcgt gcccggtccg gcggaataat
     2281 gaaaccgtga gtatcacggt ggacggggtg tcgtgatgat caatccccag gtggccaacg
     2341 tgttccacga aatcgcggac ctcctcgagc tgtccgggga caacccgttc cgaatccgtg
     2401 cctatcggcg cgcagcgatg aacgtcgaat cgatcccgaa agatgtggcc gcaatgagcg
     2461 aggaggagct tcaggccctc ccggggatcg gcaaagacct cgcccacaag atccggcagt
     2521 tcgtggacac gggccggatc gacgcccacg aggaactgca gaagaagatc ccccgcggcc
     2581 tgctcgagat tctgcgtgta ccggggatcg gcccgaagac cgcgcgcctc ctcttcgaca
     2641 aaaagaatgt gaaaagcctc gaggagcttg agacgttggc ccgctccggg aatctcaccg
     2701 gcctgccggg aatccaggcg aagaccgagg agaacatccg caaggggatc gggcagctcc
     2761 ggaaagggat ggagcggttc cctctaggca aggcgcttcc gatcgcgagg gggatcctgg
     2821 aacatctgca aaaagccgtt cccggtgccc ggttcgccct cgcagggagc atccggcgct
     2881 ggaaggagac ggtgaaggac atcgacattc tcgctgcggg aaaaatgccg aaagtgatca
     2941 cgaaggagtt cgtcgatcta ccggggacgc gtgaggtgct cgagcggggg gcgacaaaat
     3001 cctccctcct gaccgagggc gggatccagg tggatctgcg ggtagtcgcg gaggaatcct
     3061 tcggggcggc gctccaatac ttcaccggca gcaaggagca caacgtgcgc ctccgggaga
     3121 tggcgtcacg gcggggtctg aagatcaacg agtacggggt gttccgcgag gcggactcca
     3181 ggcgactcgg gggacgggag gaggaggacg tatacaaggc cctcgggctc cccttcgtac
     3241 cccccgaact gcgggaggac gccggggaga tcgaggccgc catcgagggg aggcttcctg
     3301 acctggtctc ccttgcggat atccggggag acctccacgt ccacacgaag tggtccgacg
     3361 gcgcgcacga tctcgatgcc ctggtcggag cggcgagagc acggaagtat ggatacattg
     3421 cgatcaccga tcactccaag gggctcggcg tggcgcacgg gctcaccgag gatcgggtcc
     3481 ggaagcagat ggggatgatc gatgcggcga accggaagct caaggggttc cggatcctca
     3541 aggggatcga ggtggacatc cggggcgacg gaacgctgga cctcccggat acggtgcttt
     3601 ccgagctgga tatcgtggtg gcctccatcc actcagggtt caggcaaccc ggtgagcaac
     3661 ttacgaagcg cctcgtctcc gccatccgga acccgttcgt gagcatcatc gcccacccca
     3721 ccgggaggct cctcggggag cgcgaggcat accccgtgaa catggagacg gtgatctcgg
     3781 cagcggcaaa gcatggcaag gcactggaaa tcaacgccta cccgctgcga ctggacctct
     3841 ccgacgcctg ggcccgggtg gcgatgcagc gccgcgtccc gctcgcggtc agcacggaca
     3901 cgcacatcct gagcaacctc gacttcatgg ggtacggcgt ctccatcgcc cgacgggg
//
LOCUS       DASSLD010000137         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1389897, whole genome shotgun sequence.
ACCESSION   DASSLD010000137 DASSLD010000000
VERSION     DASSLD010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389897"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1390
                     /gene="hemL"
                     /locus_tag="VFK23_02605"
     CDS             107..1390
                     /gene="hemL"
                     /locus_tag="VFK23_02605"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HET7318006.1"
                     /translation="MSGARSKKVFEEAKKHIPGGVNSPVRAFRSVGGEPLFIKKAKGS
                     KIYDADGKAYIDYVLSWGPMILGHAHPRVSAALKKAISNGTSFGAPTELEVTLAKLVK
                     KAVPSIEMVRMASSGTEATMSAIRVARGFTGRDKIVKFDGGYHGHGDCLLVKAGSGVA
                     TFGLPDSPGVPVDLAKYTLTVPYNDLAAVRDLAAREGEQIACIIVEPVAGNMGCVPPE
                     PGFLQGLRQVCDQYGIVLIFDEVMTGFRVAYGGAQQLYKIRPDLTTLGKVIGGGLPVG
                     AFGGRREIMEKVAPIGPIYQAGTLSGNPLAMTAGIETLKLLAKSGVYKALEKSSAQLE
                     QGLREAAREAGVAVTFNRVGSMFTGFFTEQKVKDFASAKTSDTARFGRFFLSMLKNGV
                     NLAPSQFEAAFMSLAHTKADIGKTVEAARKSLKGL"
     gene            1535..1783
                     /locus_tag="VFK23_02610"
     CDS             1535..1783
                     /locus_tag="VFK23_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HET7318007.1"
                     /translation="MEEKDRKLIRMLGVLSTVGLTMVFATVIGLYIGLKLDEWLGTSP
                     WLTALFLALGIAAGFRNLFVYAKRSQNELDEKDNDGKK"
     gene            1807..>2188
                     /locus_tag="VFK23_02615"
     CDS             1807..>2188
                     /locus_tag="VFK23_02615"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HET7318008.1"
                     /translation="MADESQIRRKAVSGIVKGVAKKTSLIVLCAAALSLAYSLVRPSG
                     ERWWFLPLSVLFGGALGLLNFRWLSVAVEKIYLRQGATPGLSNLAAIILSVLKLSIIF
                     IILYVVIKWQVMHIFGLVGGLTLCF"
BASE COUNT          462 a          620 c          660 g          446 t
ORIGIN      
        1 tttgccctaa aaatcaacgt ttttcattga caagcagcgc agattttcat attttatggg
       61 ggctggtttc ccggtttgac aacccgtcag cacgagagga gcatgcatgt caggagcaag
      121 atcaaagaag gtgtttgagg aggcgaagaa acacatcccg ggcggcgtga acagcccggt
      181 cagggctttt cggtctgtgg gcggcgagcc gctctttatc aagaaggcga aggggtcgaa
      241 aatctacgac gccgacggca aggcgtatat cgattacgtc ctctcctggg gcccgatgat
      301 cctggggcat gctcatccgc gcgtttccgc ggcgctcaag aaagcgatct cgaatggaac
      361 gagcttcgga gcgcccaccg agctcgaggt cacgctcgcc aagctggtca agaaggcggt
      421 cccctccatc gagatggtcc gcatggcgag ctccggcacg gaggccacta tgagcgctat
      481 cagggtggcc aggggattta cgggacggga caagatcgtc aagtttgacg gcggttacca
      541 cggccacggc gattgccttc tggttaaggc gggaagcggc gtggcgacct tcggtctgcc
      601 cgacagcccc ggcgtgcccg ttgatctcgc caagtatacg ctgacggtcc cgtacaacga
      661 cctcgcagct gtcagggacc tggccgcacg cgaaggggag cagatcgcct gcatcatcgt
      721 ggaaccggtg gcggggaaca tgggctgcgt tcccccggag ccgggatttc tccagggtct
      781 caggcaggtc tgcgatcagt acggcatcgt cctcatcttc gacgaggtga tgaccggttt
      841 ccgcgtcgcg tacggcggcg cccagcagct ctacaagata agacccgatc ttacgacgct
      901 cggcaaggtt atcggcggcg gcctgcccgt aggcgccttc ggcggccggc gcgagatcat
      961 ggagaaggtg gcgcccatcg gccccattta ccaggccggg actctctcgg gaaaccctct
     1021 ggccatgacg gcgggcatcg agacgctgaa gctcctcgca aaatccgggg tgtacaaggc
     1081 gctcgaaaaa agctcggccc agcttgaaca aggcctccgc gaagcggccc gcgaagcggg
     1141 cgtcgcggtc acattcaacc gggtcggttc catgttcacg ggatttttta cggagcagaa
     1201 ggtcaaggat ttcgcgagcg cgaagacatc cgatacggcg aggttcggaa ggtttttcct
     1261 gtccatgctc aaaaacggcg tgaaccttgc gccgtcgcag ttcgaggcgg cattcatgtc
     1321 cctggcgcac acgaaagcgg acatcggcaa aaccgtcgag gcggcgagga agagtttgaa
     1381 aggtctttaa gagcattcac cgcggagaac ccccgataac atatttcgag ggcaggctcc
     1441 gagatcgcag aggaagtcaa caggatttaa aattcaagat tcgaggtttt actctacgcc
     1501 ctttgtgccc tctgcggtaa aagacataga acttatggaa gaaaaagacc gcaagctcat
     1561 ccgcatgctg ggcgtcctga gcacggtggg gctgaccatg gtattcgcca ccgtgatcgg
     1621 cctttacatc gggctcaaac tggatgagtg gcttggcact tccccctggc tcacggcgct
     1681 cttcctggcg cttggcatcg ccgcgggatt cagaaacctg ttcgtgtatg ccaaacgcag
     1741 ccagaacgag ctggatgaaa aagataatga tgggaaaaaa tgactgattc ccgattagat
     1801 cactcgatgg cggacgaatc tcagatacga cggaaggcgg tttcaggcat cgttaaaggc
     1861 gttgcgaaaa agacctccct catcgtcctc tgcgccgcgg ccctctccct ggcatactct
     1921 ctcgtccggc cttccggaga acgctggtgg ttcctaccgc tgagcgtcct cttcggcgga
     1981 gcgctcggtc ttctgaactt ccggtggctc tccgttgctg tggaaaagat ttacctgaga
     2041 cagggagcga ccccggggct ttcgaacctt gccgcgatca ttctcagcgt cctcaagctt
     2101 tcgatcattt tcatcattct ctacgtcgtc atcaaatggc aggtcatgca catcttcggt
     2161 ctggtgggag ggcttacgct ctgcttcc
//
LOCUS       DASSLD010000138         3724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1617876, whole genome shotgun sequence.
ACCESSION   DASSLD010000138 DASSLD010000000
VERSION     DASSLD010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617876"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..746)
                     /locus_tag="VFK23_02620"
     CDS             complement(156..746)
                     /locus_tag="VFK23_02620"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HET7318009.1"
                     /translation="MPEVELPDLEELKESAGKRFSKRVALTTALYAVLLAITSLGGNN
                     ATKEMMLHQQMSSNQWSYYQAKSIKESFARERRESIEAILAERGAMMKPDARVAYERM
                     RETTTSDEARYAKEKEQILAQAKALEKERDVSRAKDPYFDFGEVLLQLAIVTASIAIL
                     SGSSPVYLFSIVMASLGVALSLNGFLLLVTLPFLTP"
     gene            complement(887..1324)
                     /locus_tag="VFK23_02625"
     CDS             complement(887..1324)
                     /locus_tag="VFK23_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HET7318010.1"
                     /translation="MKWVTREDAKVDRIACPWLIRRFVDKDAVFLYVPADQVLSVARR
                     EGANPYDVPGVELGHVDGRCSFESILHKYGLGNDPALVELAKIVHAADVEADIDSSPE
                     GRGLQAIAKGFSILHGKNDHRKIELETPMYDALYAWCRERARI"
     gene            complement(1500..1886)
                     /locus_tag="VFK23_02630"
     CDS             complement(1500..1886)
                     /locus_tag="VFK23_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318011.1"
                     /translation="MEKKLTITVEIDGDRKEIVVRDSAGNSRHLKSVLVTGGGVEEGS
                     LYLFGWGSCADLGWALAEGFRYSSESPRSTDAFYQRVFAHLARWIAKFHGWALEQPVD
                     PRMLLERWEAEDAAMSAEEACRNSTN"
     gene            2242..3543
                     /locus_tag="VFK23_02635"
     CDS             2242..3543
                     /locus_tag="VFK23_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET7318012.1"
                     /translation="MRRYFDFERHGTTYRKETLAGITTFMTMAYIIIVNPAILEAAGM
                     PRGPSMVATILSAAFGTVVMGLYARRPFAIAPYMGENAFIVFTVVKVMGYSWQAALGA
                     VFVAGVLFTLLTVLKVRSWLAEALPKSLKFSFSVGIGLFLAFIGLNETGIVRLGVPGA
                     PVSLGNLAEARALLSLFCLFLIVLMRIRRIPGAIVTGVLCTTFLSFLFNVTPAPVRWI
                     GFPPDVRPLLLKLDLSATLSPSFFPIILTIFVMAFVDTVGTLIGLSARAGLLDEKGNL
                     PDIEKPMLADALATTVAPLLGTTTTGAFIESAAGIEEGGRTGFTSLVVAVLFLLSLFL
                     APLLTSIPPHAYGAALIVIGSFMIAPVTKIDVNDPTELIPAFLTISLISFTFNIGVGM
                     TAGIISYPLLKVFSGQIREVPAGLWVLAVLSLTFYIFYPYR"
BASE COUNT          678 a         1156 c         1033 g          857 t
ORIGIN      
        1 attctttggc atttttattt tgggctcctc ctccgtccct ggatcaggca tgaagcattt
       61 atctgtattt tcagtacata ctgaaatata tatccatact gattaattgt caatacctgt
      121 catgcacctg gcctgaaacc agggagcagg tcgagtcacg gagtcaggaa cggaagggtg
      181 accagcagca ggaaaccgtt caacgacaac gccacgccga gcgacgccat gacaatcgag
      241 aacaggtaga cgggggacga cccggacagg atcgcgatcg acgcggtgac gatcgcgagc
      301 tgcagcagga cctccccgaa atcgaaatac ggatccttcg cgcggctgac atcacgttcc
      361 ttctcgagcg ccttcgcctg tgcgaggatc tgctccttct ccttggcgta tcgcgcctcg
      421 tcggacgtcg tggtctcccg cattcgctcg tacgcaaccc gggcatccgg cttcatcatc
      481 gcgccacgct cggccaggat cgcctcgatc gactcgcggc gctccctggc gaacgactcc
      541 ttgatcgact tcgcctgata gtacgaccat tggttgctgg acatctgctg gtgcagcatc
      601 atttccttcg tcgcgttgtt cccgccgagc gacgtgatcg ccagcaggac ggcgtaaagc
      661 gccgtcgtca gcgcgacgcg cttcgagaac cgtttccccg ccgactcctt gagctcctcc
      721 aggtccggaa gttccacttc tggcatcggt gctcgatacc tccttttttt gcttcgcaaa
      781 ccacggtggc ggcgcgcaag cgatcccatc cagggatcga gatcgaggag gaccaggagg
      841 acacgggtga cattcaccgt cccccttgtc cccgagaccg ttcccgtcag atcctggccc
      901 tctccctgca ccaggcgtag agggcatcgt acatcggtgt ctccagctca atcttccggt
      961 ggtcgttttt cccatggagg atggaaaacc ccttggcgat ggcctgaaga cctcgtcctt
     1021 ccggggacga gtcgatgtcg gcctcaacat ccgccgcatg gacgatcttc gcaagttcga
     1081 caagcgccgg gtcgttcccc aggccatact tgtgcaggat gctctcgaac gaacagcgcc
     1141 cgtctacatg gcccaactcg acgccgggga cgtcgtacgg gttggctccc tcccgccgcg
     1201 cgacggacag tacctgatca gccgggacat agaggaacac cgcatccttg tccacgaacc
     1261 gccggatgag ccacgggcat gcgatccggt ctactttcgc gtcttctctt gttacccatt
     1321 tcatggctct accttccgct ccaggctgcc ggtcaatcgg ggatcccggt cccagtcttc
     1381 aagcgcttga cctttttcgt gagatgcccg gactgttggc aaaggtccgg attccaccgt
     1441 atctctcggc gatcttcggt ccgcattcgt cgggggatcg gttgagggat cgaagggtct
     1501 taattcgtgc tgtttcggca ggcctcttct gccgacatcg ctgcatcctc cgcctcccac
     1561 cgctcgagga gcattcgcgg gtccacgggc tgttcgagtg cccagccatg aaatttggcg
     1621 atccaccgcg ccaggtgtgc gaatacccgc tggtagaatg catccgtgga gcgaggggat
     1681 tcgctggagt atcgaaaacc ttccgccaag gcccatccaa ggtcggcgca ggatccccag
     1741 ccgaacaggt aaagggagcc ttcctcgaca ccaccgcccg tcacaagaac gcttttcagg
     1801 tgacggctgt ttccggcact gtcccgtacg acgatttctt tccggtcgcc atcgatctca
     1861 accgtgatgg tcaatttttt ttccatggct tttccttctt ctgaaaagct tttttctacc
     1921 cccggaattg tcagttcgaa caatctccgt ataatttatc ggcatgaatt tcaagaaagt
     1981 tcaatttcga aatattgcat ggaaagggtt ttcttccaaa tagctgataa tgaaaggaat
     2041 atatccggaa ataccccccc ttctccccga aaaaaaaccg gttgtttgca cggggtttgc
     2101 ggtccatccg gattaccggt acaaaatgcg agagagacgt tcaggcaatt gttacattaa
     2161 gatctgggag gactgatcac cggtggcaag ccgccggggc cttgagttgc gctgatagcc
     2221 agagacgggg ggaggctgcg cgtgaggcgg tacttcgact tcgaacggca cggtacaacc
     2281 taccggaagg agacattggc ggggattacg accttcatga cgatggcgta catcatcatc
     2341 gtcaatcccg cgatcctcga agcggccggt atgccgaggg ggccctccat ggtggcgaca
     2401 atcctctccg ccgcgttcgg caccgtcgtt atgggtctat acgcacggcg tcccttcgcc
     2461 atcgccccgt acatggggga gaacgctttc attgtcttca ccgtggtcaa ggtcatgggg
     2521 tattcctggc aggccgcgtt gggcgccgtc ttcgtcgcgg gagtactgtt caccctgctg
     2581 acggtactta aagtccggag ctggctggct gaggcgctcc cgaaatccct caagttcagt
     2641 ttctccgtcg ggatcggcct gttcctggcg ttcatcgggt tgaacgagac cggtatcgtc
     2701 cggctgggcg ttcccggagc gcctgtgtcc ctggggaacc tggcggaggc ccgggcgctg
     2761 ctgtcgctgt tctgcctctt cctgatcgtc ctgatgagga tccggcgcat cccgggtgcg
     2821 attgtcaccg gcgtgctgtg caccactttt ctgtcgttcc tattcaacgt cacgcccgct
     2881 cccgtccgat ggatcggttt tccccccgat gtgcgccccc ttctgcttaa gctcgacctg
     2941 tcggcaaccc ttagcccgag cttttttccg atcatcctga cgattttcgt catggccttc
     3001 gtggatacgg tggggacgct gatcggcctg tccgcgcggg cagggctgct ggacgagaag
     3061 gggaacctgc ccgatatcga gaaaccgatg cttgcggacg cactggccac cactgtggcg
     3121 ccccttctgg ggacgacgac cacgggagcc ttcatcgaat cggcggccgg gatcgaggag
     3181 ggggggcgga ccggcttcac ttccctcgtc gtggccgtcc tgttcctgct ctctctgttc
     3241 cttgctcctt tgctgacctc tatcccaccc cacgcctacg gggccgcact catcgtcatc
     3301 ggcagcttca tgatcgcccc ggtcacgaag atcgacgtta acgatcccac ggagttgatc
     3361 cccgctttcc tcacaatctc cctcatcagc ttcaccttca acatcggtgt aggtatgacg
     3421 gcagggatca tctcctaccc gctgctgaag gttttcagcg gccagatccg ggaggtccct
     3481 gcaggtctgt gggttctggc ggtcctttcc ctgactttct atatcttcta tccataccgg
     3541 tagacaattc cccgggaatt ccagctagcc gctggatcgg ttaagcgggg agatgcccac
     3601 ctcgccccgg gttatcgcag gaagacgatc ggcaagtaca ccgaggtcgt cgaggtcacc
     3661 gtttcatagt cgtagccagt ttcgccggtg cctccaaagt cgctacttcg ccgggaggat
     3721 catt
//
LOCUS       DASSLD010000139         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1072875, whole genome shotgun sequence.
ACCESSION   DASSLD010000139 DASSLD010000000
VERSION     DASSLD010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072875"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..>2302
                     /locus_tag="VFK23_02640"
     CDS             325..>2302
                     /locus_tag="VFK23_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET7318013.1"
                     /translation="MTTRKVEQNGAACKENVFIQVAGVMNGSPAAKAGLQEDDVILTL
                     GGSPTCTDGGDTMLSFKKLIERQKTGVPLSLDILRKDQKFSLTVVLEEMPVNDQPEAN
                     HPGLEQCPERSSTLQRALGAENALPLFNTISNELYRRSNLAHNPGSAFEKKSNPLQLR
                     EVTYLMRHPQAAGEAARELSERISTPLSERDWQAGEVVRKAASLLDIELAPSLRLSEI
                     TFPALLHVMEETKKDVEKALGNLTKEEKELLQKAANDPTDDSQWNKVLDVSMKVDREG
                     LFRAFSPLLSFLTRDNLALLREDLAVRFPSKKGDILYEAMTPVGKVIVGGAGPNVYGD
                     DCALILDLGGDDLYLNNAGGSRPGMPVSLVIDFGGNDRYVGRENFSQGAGLLGGGFLL
                     DLGGSDTFVGLTGSQGAGFFGLGLLYHGDGDAGFSASRYSQGTGMMGIGLLTSRKGDS
                     KYLCSFGGQGLGLFGGAGVLIDEEGNDFYQLGGREPDFRDPQKATQSFGQGFGLGVRS
                     ERGVNAAPGGIGMLIDEQGNDTYLADYFAQGASYYYGLGILDDRAGDDQYFAGRYAQG
                     AGIHSSVGVLIDREGDDFYYSSYGVGQGMGHDYGVGFFEDDGGENRYQAGTLVQGAAT
                     NGSIGILIDPNGPDKLSYQSSGQAYAESENSMGIV"
BASE COUNT          509 a          644 c          701 g          448 t
ORIGIN      
        1 cagagagcac cgaggacccg gagaaatacg agaactcggg acgtaaaaat attcccaaac
       61 tctgtggtcc caaagcttca ccagctctgc ataatgtgcg tcgacgactc ttcatctgtt
      121 ccgatcatgc acaataacac caccatgccg aaatattata ttcctgcgat ttctctcgtt
      181 ctctgcctca tgtcctgcgc cggctatccg tcactcaccc gggaggccgc gccctcggcc
      241 gggcttcagc cctattgcga tctgctccat atgccatcaa ggccctatcc tccaatcgaa
      301 gcgagaccct acctcgggtg ctatatgact acccgcaaag tggaacaaaa cggagcggcg
      361 tgtaaggaga acgtctttat tcaggtcgcc ggggtcatga acggatcacc cgcggcaaaa
      421 gccggtctgc aggaggatga cgtcatactg acgctcggag gcagccccac ctgtaccgat
      481 ggaggcgaca ctatgttgtc cttcaagaaa ctgatcgagc gtcaaaagac cggtgttccg
      541 ctcagccttg atatcctgcg caaggaccaa aagttttccc tgaccgttgt actggaagag
      601 atgcccgtca acgaccagcc ggaggcaaac caccccgggc ttgaacaatg ccccgaacga
      661 agctcgacgc ttcaacgcgc gctcggcgct gaaaacgcgc ttcccctgtt caacacgatt
      721 tcgaacgaat tatacaggag gtcgaacctc gcgcacaatc ctggctcggc tttcgaaaaa
      781 aagtccaacc ctcttcaact tcgggaagtg acctatctga tgaggcaccc ccaggccgcg
      841 ggcgaagcgg ccagggaact gtcggagcgg atatctactc ctctttcgga acgtgattgg
      901 caggcgggag aggtcgtgcg aaaggccgcc tcgctgcttg atatcgagct tgctccatct
      961 ctgcggctgt cggagatcac gttccccgca ttgttgcatg tcatggagga gacgaaaaaa
     1021 gacgtcgaaa aggcgctcgg caatctgacc aaggaagaaa aggagctctt gcagaaggcc
     1081 gcgaacgatc ccacggacga cagccaatgg aacaaggtac tcgacgtttc gatgaaggta
     1141 gaccgcgagg gactgttccg ggcattttcc ccgctcctct ccttcctgac ccgtgataat
     1201 ctggcgctgt tacgggagga cctcgcggtc cggtttccct caaagaaggg cgacatactc
     1261 tacgaagcca tgacgcccgt cggcaaggtg atcgtgggtg gcgcggggcc gaatgtctac
     1321 ggggacgatt gcgcgctgat cctcgacctc ggcggagacg atctctacct gaacaacgcg
     1381 ggagggagcc gccccgggat gcccgtctct ctcgtgatcg actttggagg gaacgatcgt
     1441 tatgtgggca gggagaattt ctcgcagggc gcggggcttc tgggaggagg ctttctcctg
     1501 gatctcggcg gcagcgacac gttcgtcggg ctaacgggaa gccagggagc gggtttcttc
     1561 gggctgggac tcctctatca cggcgacgga gacgccggat tcagcgcgag ccggtattcc
     1621 cagggtacgg gaatgatggg catcgggctc ctgacgagca gaaaaggaga cagcaagtat
     1681 ctctgctcct ttggcggaca gggtctcggt ctctttggag gagcgggcgt cctgatcgac
     1741 gaagagggga acgattttta ccagttggga ggccgggagc cggatttccg cgatccccag
     1801 aaggccaccc agagctttgg ccaggggttc ggattaggag ttcgttcaga gagaggtgta
     1861 aacgccgcgc cgggcggtat cggcatgctt atcgacgaac agggaaacga cacgtacctc
     1921 gcggactatt tcgcccaggg cgcgtcgtat tactatggcc tgggcattct ggacgacagg
     1981 gccggcgatg accaatactt tgccggccgc tacgctcagg gcgcagggat tcactcctcg
     2041 gtcggggtcc tcatcgaccg ggagggggat gatttctact attcgtccta cggcgtgggg
     2101 cagggcatgg ggcatgatta cggcgtcggt tttttcgagg atgacggagg ggagaaccgc
     2161 taccaggctg ggaccctggt ccagggcgcg gcaacgaacg gaagtatcgg catcctgata
     2221 gacccgaatg gaccggacaa actgtcctat caaagcagcg gtcaggcata tgcggaatca
     2281 gagaacagca tggggattgt ga
//
LOCUS       DASSLD010000140         3822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1259176, whole genome shotgun sequence.
ACCESSION   DASSLD010000140 DASSLD010000000
VERSION     DASSLD010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259176"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1146)
                     /gene="queA"
                     /locus_tag="VFK23_02645"
     CDS             complement(103..1146)
                     /gene="queA"
                     /locus_tag="VFK23_02645"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET7318014.1"
                     /translation="MISSAVEQLRLADFDYDLPAERIAQEPSPERDRARLMIMDRGSG
                     AVQHRIFSDIDACLRPGDLLVMNDTRVFPCRLAATKPGGGKAEIFLLAEQERNVWHAL
                     VKGGPGPGKRLCCAAGAEAEILRDHADGSRTVRFHGVRDIRDVLGEIGSVPLPPYIKR
                     PATDRDRERYQTVYASREGAVAAPTAGLHFTKALLARLAAKGVEQASVTLHVGPGTFQ
                     PVRTENISLHRMHPEKFSISASAAERINRARAEGRRVIAVGTTSVRTIETASQKTGAV
                     ESGEGESALFIYPGYRFRATDGIITNFHLPKSTLLMLVSAFAGRERVLAAYRTAVAER
                     YRFYSYGDAMVIL"
     gene            complement(1291..2442)
                     /locus_tag="VFK23_02650"
     CDS             complement(1291..2442)
                     /locus_tag="VFK23_02650"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7318015.1"
                     /translation="MKEKSKTMKLIRRSVIFIVVLVIPSLSAGPAFSQSAEQNTYARH
                     VREGMAQMKSGDYRAAQDSFESAVRYYDSGIEAHAGLGMVYYNQREDRSAERELKRVV
                     ELDPRNAGAYEVLGEISYRNDDLETAISYWETAVTLDPSAARLRERLERVRKEHRTEK
                     DFSRDVTGHFLTKCEGREKIEAGRIILRILEDAYAEVGRALSFYPDREIQVILYSGEQ
                     FREVTDAPGWSAGIYDGKIRIPVGGIEQETPGLRRILYHEYTHAVVRSLTARCPTWLN
                     EGLAQYFEGREVDSRQRGALRRTAREGRLPSLSSLEGSFTGLDADRAAHTYLVSLSAV
                     RYMIDSFGLYRVRYVLDELAAGADAGKALTAGLALSSEEFERGWKKSLE"
     gene            complement(2509..2907)
                     /locus_tag="VFK23_02655"
     CDS             complement(2509..2907)
                     /locus_tag="VFK23_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005486990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET7318016.1"
                     /translation="MIRQLRVKTNSRTELVDITQGVQRLVAESGVRSGVCYVYVPHTT
                     AAVTINENSDPNVGRDILKELNKVIPFDDAYGHTEGNSAAHIKSSIIGVSRSVIVEEG
                     RLALGTWQALFFCEFDGPRDRRVYVKVMRD"
     gene            complement(3009..>3822)
                     /locus_tag="VFK23_02660"
     CDS             complement(3009..>3822)
                     /locus_tag="VFK23_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HET7318017.1"
                     /translation="ANTLFNLIISLVFLPLTGLFAKLITRLVPDKPEEKKFGPMYLDD
                     HVLGTPALALGQASREALRESDIVREMLTEVVHALQTDDQSVIEEIKNKDNLIDVLDR
                     HIRLYLTRLSSSRLSESQSRRAMAVLEMARNLESIGDIIDRNIMPLAVKRIVKGFTFS
                     QEGMDEIAAFHKKVLDNFDTAISAFATNDRDLADRVLRHKEELGAVERELIQTHFDRL
                     RRGYRESLETSHIHLDIIGNLARINSLITHIIYPIVEEKRGRGREDVGIEER"
BASE COUNT          717 a         1198 c         1109 g          798 t
ORIGIN      
        1 cgaccgccgg gataagaagc aacttggata gaatgccgcc gcgttcgttg ttcgtcatgc
       61 agttcttccc ttccgaaaac attccgcaat ccgaattccg cattataaaa tgaccatcgc
      121 atccccgtag ctgtagaacc ggtatcgctc cgccaccgcg gtccggtagg ccgcaagcac
      181 ccgttccctt ccggcaaagg cggacacgag catgagcagc gtcgatttcg gaagatggaa
      241 gttggtgatg atgccgtcgg tcgcccggaa gcggtatccg gggtagatga acagcgccga
      301 ttccccctcg cccgactcga cggcgccggt cttctgcgaa gctgtttcga tcgttcgaac
      361 gctggtcgtt ccgacggcga tcacacgcct tccctccgct cgtgcgcggt tgatcctttc
      421 ggccgctgac gcggagatcg aaaatttttc cgggtgcatt cggtgcaggg agatgttctc
      481 ggtgcgcacg ggctgaaagg taccaggccc cacgtggagc gtcaccgagg cttgttcgac
      541 ccccttcgcg gccagtcttg caagcagcgc cttcgtgaag tgaagacccg cagtcggggc
      601 cgccaccgcg ccctcccgcg acgcgtacac agtctgatac cgctcgcggt ccctgtccgt
      661 ggcaggccgt ttgatgtagg gaggaagcgg aacgctgccg atttctccca gaacgtcccg
      721 gatgtctcga acgccgtgga aacggaccgt ccgtgaccca tcggcatgat cccgaaggat
      781 ttcggcctcc gctcccgccg cgcagcaaag ccttttcccc gggcctggtc cgcctttcac
      841 gagggcatgc cagacgttgc gctcctgttc ggccagaaga aatatctcgg ctttgccgcc
      901 gcccggcttc gtcgccgcga gcctgcaggg gaagaccctc gtgtcgttca tgaccagaag
      961 atcgccgggc ctcagacagg catcgatgtc cgaaaatatc cggtgctgaa cggcgcccga
     1021 cccgcggtcc atgatcatga ggcgcgcgcg gtcacgctcc ggagaaggct cctgagcaat
     1081 gcgttcggcg ggaaggtcgt aatcgaaatc ggcaagcctc agctgctcca cggcggaaga
     1141 aatcatggcg ggataatacc ataggacggg acaaaatcca aattacaaat tgcaaatccc
     1201 aaataaattc caagtacaaa acgtttggaa tttgtctctt ggtatgtggg attgtattcg
     1261 agatttggtg cttgtcattt ggaatttgtt ttactccagc gacttcttcc agccccgctc
     1321 gaactcctcc gaggacagtg caaggcccgc ggtgagcgcc ttgcccgcat ccgcacccgc
     1381 tgccagttca tcgagaacgt atctcacgcg atagaggccg aatgaatcga tcatataccg
     1441 caccgcggag aggcttacca ggtaggtgtg cgccgcccgg tccgcatcca agcccgtgaa
     1501 cgatccttcc aatgacgaaa gcgacggcag ccttccctcc cgggcggtcc gcctgagcgc
     1561 gccccgctgc cgtgaatcga cctccctgcc ttcgaagtat tgcgccagtc cttcattcag
     1621 ccaggtgggg cagcgcgccg tgagggagcg cacgacggca tgggtgtatt cgtggtagag
     1681 gatcctcctg agcccaggcg tctcctgctc gatgccgccg acgggaatcc ggatcttgcc
     1741 gtcatagatg cctgcgctcc atccgggggc gtccgtcact tccctgaact gctccccgga
     1801 atacagaatg acctggatct cgcgatcggg atagaaggac agggcgcggc ccacctcggc
     1861 gtaagcgtcc tcgaggattc tgaggatgat cctgcccgcc tcgatcttct ccctgccttc
     1921 acatttagtg agaaaatggc ccgtaacatc gcggctgaaa tccttttccg tcctatgctc
     1981 tttccggacg cgttcgagcc tctcgcgaag gcgcgcggct gaggggtcca gcgtcacagc
     2041 cgtctcccag tacgaaatcg cggtctcaag gtcgtcattg cggtaggaga tctcgcccag
     2101 gacctcatag gcccccgcgt tccggggatc gagttccacg acgcgcttca actcccgctc
     2161 cgccgagcgg tcctcccgct ggttatagta gaccatgccg aggccggcat gggcctcgat
     2221 gccggagtcg taataccgga cggcgctctc gaaagagtcc tgcgcggccc ggtaatcgcc
     2281 gctcttcatc tgcgccatcc cctcccggac gtgccgcgca taggtattct gctccgcgga
     2341 ttgtgaaaaa gccggacccg cggagagcga cggtatgacg agaacaacaa taaatatcac
     2401 gctccgcctg atcagcttca tggtcttgct cttctctttc actccgaact ccgaactgtg
     2461 aacggagcct gcccccgaat gtctttatcg ggggaactcc caactgaatc agtccctcat
     2521 caccttcacg tagacccgcc ggtcccgggg tccgtcgaac tcgcagaaaa agagggcctg
     2581 ccacgtgccg agcgcgaggc ggccttcttc cacgatcacg gaccgggaga cgccgatgat
     2641 gctcgatttg atgtgcgcgg ccgaattccc ctcggtgtgg ccataggcgt catcgaaggg
     2701 gatgaccttg ttcagttcct tcaggatgtc gcgccccaca ttcgggtccg aattttcgtt
     2761 tatcgtcacc gccgcggtcg tatgcggcac gtaaacgtag cagacgccgg agcggacccc
     2821 gctctcggcc acgagacgct gcactccctg cgttatgtcg acgagctccg tcctggaatt
     2881 cgtcttgact ctgagctgtc tgatcatgcc ccccccgttc gaatgcggaa tgcggaatgc
     2941 ggaataaaac ccaaagccat agtagccttc ttttcattcc gcattccgaa ttccgcattc
     3001 caaatttatt atctctcttc tatccccacg tcctcgcgcc cccttcccct cttctcctcc
     3061 acgatcgggt agatgatgtg cgtaatgagg gaattgatgc gcgccaggtt gccaatgatg
     3121 tccaggtgga tgtggctcgt ctcgagcgac tcgcggtagc ccctgcggag ccggtcgaag
     3181 tgggtctgga tgagctcgcg ctccacggcg ccgagttcct ccttgtgccg gagcacgcgg
     3241 tccgcgaggt cccggtcgtt ggtcgcgaag gcggaaatgg ccgtatcgaa attatcgagc
     3301 accttcttat ggaacgcggc gatttcatcc atcccctcct gggaaaaggt gaaccccttg
     3361 acgatccgct ttaccgcaag gggcatgatg ttccggtcga tgatgtctcc gatgctctcg
     3421 aggttgcggg ccatctcgag aactgccatc gctcggcggg actgggactc ggagagacgc
     3481 gaggatgaca gccgggtgag atacagccgg atgtggcggt ccaggacgtc gatcaggttg
     3541 tccttgtttt tgatttcctc gatcacggac tggtcgtcgg tctgcagcgc atggacgact
     3601 tccgtgagca tttcccggac gatgtccgat tcgcggagcg cttcgcgcga tgcctgtccc
     3661 agcgcaagtg caggggtgcc caggacgtgg tcgtccaggt acatgggccc gaacttcttc
     3721 tcttccggct tgtccggcac cagccgcgtg atcagcttcg cgaacagccc ggtgagcggc
     3781 agaaacacga gggagatgat caggttgaac agggtgttgg cg
//
LOCUS       DASSLD010000141         3532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_775301,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000141 DASSLD010000000
VERSION     DASSLD010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775301"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VFK23_02665"
     CDS             <1..427
                     /locus_tag="VFK23_02665"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HET7318018.1"
                     /translation="LIFLHAMAMSEGTDNSIDNIVYNFSPDRSRWNNYAIDFDDTIYY
                     YDTKSVTKKNNNVKVWIKFGEPISDKKDTRIYKEAMALKEIDCSTRLIRSIEWNYISM
                     KNEFNKYTSPTKWEEIEPETADDALIDEVCTRPKKVKKR"
     gene            complement(468..1139)
                     /locus_tag="VFK23_02670"
     CDS             complement(468..1139)
                     /locus_tag="VFK23_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HET7318019.1"
                     /translation="MCGRFTLFEADQILSKEFGVSGFPPQSPRYNIAPSQRVAAVRVA
                     PEEGGRELALLRWGLIPSWSKDPSIGNRLINARAETAREKPSFRNAFRRHRCLIPANG
                     FYEWQRQERGKQPYYVRMRDERLFAFAGLWEKWESPDEGAIETCTILTTAANAVLAPI
                     HDRMPVILPSTAYARWLDPALRDADSLVPLLVPFPPEDMLALPVSPRVNAPTVDDEKC
                     IAPLS"
     gene            complement(1302..2207)
                     /locus_tag="VFK23_02675"
     CDS             complement(1302..2207)
                     /locus_tag="VFK23_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET7318020.1"
                     /translation="MFDVVGIGLNAVDYLVGLPRFPIPGEKLRMSGFTREGGGQVATA
                     LVALSRWGLRCKYVGNVGDDENGRISSLLLSNEGIDLAHVRTVPGASSQFAVILVEEK
                     TGERTILWDRDPKIRVSREDLPLDEIRRARALLLDGHDVPPSIIAARTARAAGVPVVL
                     DAEKVQEGTEELLPLCDHIVAAGHFPGLLIPGVSAENGAREMLRRYAPRTSTVTLGPR
                     GAFGCDGREEIFSPSIRVDALDTTGAGDVFHAGFLYALLEGLPFREILAFANTAAGLS
                     CRGMGGRSAIPTIEEIQAANASPDL"
     gene            complement(2289..3242)
                     /locus_tag="VFK23_02680"
     CDS             complement(2289..3242)
                     /locus_tag="VFK23_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7318021.1"
                     /translation="MKKRKLGNSGLVVAPLAFGGNVFGWTADEPTSFRLLDAFVAAGC
                     NLIDTADIYSAWVPGNKGGESETVLGKWLKASGNRRKVLIATKVGMEMGPNRKGLSKS
                     YILRAAEDSLRRLRTDYIDLYQSHADDPETPLEETMEAYAQLIRQGKVRAIGASNYTA
                     DRLTQALEVSARHGYPTYQGLQPLYNLYDRADYEKELEPLCLEKGVGVIPYSSLASGF
                     LTGKYRSEKDLLNRPRGESVKKYLNARGFRILGALDQVAAQHHSVPAVVSLAWLIARP
                     SVTAPIASATSLDQLTDLIEATKLELAPSSIELLNMASAVE"
BASE COUNT          777 a          990 c         1043 g          722 t
ORIGIN      
        1 attaatcttc ctgcatgcca tggcaatgtc cgaaggtacc gacaattcaa tagataatat
       61 tgtatataat ttttctcctg atcgtagcag atggaataat tatgcgatag attttgacga
      121 tactatatat tattacgaca ctaaaagtgt aacgaaaaaa aataataatg taaaggtgtg
      181 gataaaattt ggagaaccaa taagcgataa aaaagataca cgcatataca aggaagcaat
      241 ggcattaaaa gaaatagatt gcagcactcg cttaattaga tctatagaat ggaattatat
      301 atctatgaaa aatgaattta ataaatacac ttctccgacg aaatgggagg aaattgaacc
      361 tgaaaccgcg gatgatgcgt taatagatga ggtgtgcaca cggccaaaga aggtaaagaa
      421 aaggtaaaac agggaacgtc cctgacggat gtccggtcgt cggatggcta cgaaagaggg
      481 gcaatgcact tctcgtcgtc gacggtgggg gcattgaccc ggggactcac gggcaaggcg
      541 agcatgtctt ccggtgggaa cggaacgaga agcgggacca gagagtccgc gtctcgtagc
      601 gccggatcga gccaccgtgc gtacgccgtc gaaggcagga tcaccggcat ccggtcatgg
      661 atcggggcca ggacggcatt cgcagcggtc gtcaggatcg tgcatgtttc aatggcaccc
      721 tcgtcggggc tttcccactt ttcccacagg ccggcgaagg caaagagccg ctcatcgcgc
      781 attctgacat agtacggctg cttcccccgc tcctgccgct gccactcata gaatccgttg
      841 gcagggatca ggcaacgatg ccggcggaac gcattacgga aggacggttt ttcacgggct
      901 gtttccgcac gggcgttgat gagccggttg ccgatcgacg ggtctttcga ccaggaaggg
      961 atgaggcccc agcgaagaag ggcaagctcg cgcccgcctt cctcgggggc gacgcgtacg
     1021 gcggcaactc gctgggaggg ggcgatgttg tagcgcggcg actgtggggg gaagccggag
     1081 acgccgaact ccttggaaag gatctggtcc gcttcgaaca gggtgaaccg gccgcacatc
     1141 gatcgacctc catttcgacg aaagtattgt ttcccatccc accaggttcc atctacatta
     1201 tcgtgcaaga agtcgatggg ttgcggtggg aagaatcggt atccggcgag aacctgttga
     1261 ccggacggcg gggaacccct catgaggcag acgtcgcttt cctacaggtc cggtgatgcg
     1321 ttggccgctt ggatctcctc gatcgtcggg atcgctgacc gtccccccat gccgcggcag
     1381 gagaggccgg ctgcggtgtt ggcgaaggcg aggatctccc ggaaggggag cccttcgagg
     1441 agggcgtaca ggaacccggc gtggaagacg tccccggcgc ccgtggtgtc caacgcatcg
     1501 acgcggatgg aaggcgagaa gatttcttcc cggccgtcgc atccgaacgc tccgcgaggg
     1561 ccaagcgtca ccgtggacgt cctgggggca taacggcgca acatttcccg ggcgccattt
     1621 tcggccgaga ccccgggtat cagcaacccc gggaagtgtc ccgcggccac gatgtgatcg
     1681 cacagtggca ggagttcctc cgttccctcc tgaacctttt ccgcgtcgag aacgacgggg
     1741 acgccggcgg cccgggcggt cctcgccgcg ataatcgacg ggggtacgtc gtggccgtcg
     1801 agaaggagcg cccgcgcccg ccggatttcg tcaagcggca ggtcctcccg tgatacccgg
     1861 atcttcggat cccggtccca caggatggtc cgctcgcctg ttttttcctc cacgagaatc
     1921 accgcgaatt gggacgaggc gccgggaacc gtccggacat gggcgaggtc gattccctca
     1981 ttcgaaagga gcagggacga aatccgtccg ttctcgtcat ctcccacgtt cccgacatat
     2041 ttgcaccgca ggccccaacg ggatagcgcg acgagggcgg ttgccacctg gccgccgccc
     2101 tctcgcgtga aacccgacat acgcagcttc tcgcccggta tggggaacct cggcaggccc
     2161 acgaggtaat cgaccgcgtt gagtccgatt ccgacaacgt cgaacaccgg ttgcctcctt
     2221 tcgcccaact ggacccaaga tctgccgagg ttcgacaggg acgagaacgc gaagattctt
     2281 gagagagctt actccaccgc ggaagccata ttgagcaact cgatggaaga gggggccagc
     2341 tcgagtttcg tcgcctcgat cagatcggtc aactggtcca ggctcgtcgc gctcgcaatg
     2401 ggtgcggtga cgctgggacg cgctatcagc caggcaaggg aaacgaccgc gggaacggaa
     2461 tgatgttgag ccgccacctg atccagtgcg ccgagaatgc gaaaacccct ggcgttcagg
     2521 tatttcttga cggattcgcc tcgcggccgg ttcaaaagat ccttttccga ccggtatttc
     2581 ccggtgagaa atccgctggc aagcgaggag taggggatca cgcccactcc cttttccagg
     2641 caaagaggtt cgagctcctt ttcataatcc gcacgatcgt agagattgta taaaggttgc
     2701 aggccttggt aggtggggta tccgtgtcgt gcgctcactt ccaatgcctg cgttagtcgg
     2761 tccgcggtgt aattcgaagc gccgatcgcc cggacctttc cctgccggat caattgcgca
     2821 taggcttcca tggtttcttc caatggcgtc tcggggtcgt cggcatggga ctggtacaga
     2881 tcgatgtagt ccgtccgcag gcgcctcagg gaatcctccg ccgcgcgcag gatatacgat
     2941 ttcgagagac ctttccggtt cggtcccatt tccattccga ccttggtggc gatgaggacc
     3001 ttcctgcggt taccgctcgc tttcagccac ttgccgagta cggtctcgga ttcaccgccc
     3061 ttgttccccg gaacccaggc ggagtagata tccgccgtat cgatcaaatt gcatcccgcc
     3121 gccacgaacg cgtccaaaag tctgaaggat gtcggttcat cagcagtcca tccgaagaca
     3181 ttgcctccaa atgccagcgg agcgaccacc agtccggaat tccccaattt tcgtttcttc
     3241 atgggatcgt ttccccccgg gtaatgacta ttagagaaca ggcatggggc ggaggttccg
     3301 atacggaggc gtaatcaccc acctcagaag tcgattccgg caagttgcct tggccgaagg
     3361 atatttgacc ttgccccgtt tttaacggat acccgagtct gttttctacc gaacagcgtc
     3421 taatccatta tgccgccatc gtcatgaaaa gggagattgg ccatgagtac gacaaatccc
     3481 gatcgcatcg aggaaaaggt tctgcttcgc acgtcccggt cgcgtgtttg gc
//
LOCUS       DASSLD010000142         2840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_443438,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000142 DASSLD010000000
VERSION     DASSLD010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443438"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1357)
                     /gene="rimO"
                     /locus_tag="VFK23_02685"
     CDS             complement(53..1357)
                     /gene="rimO"
                     /locus_tag="VFK23_02685"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET7318022.1"
                     /translation="MVSLGCPKNQVDAEQMLGVLAASGFEITPDQAEANVIIVNTCGF
                     IESAKEESIEAVLDAARMKTEGRCAKVVVSGCLAQRYREELLRELPEADAVIGTAEVG
                     RIAEICGEALSGKEQLLRVPPPAMVYGLPRLSTTPLHYRYLKIAEGCSNRCSYCAIPL
                     IRGNFKSRPSASVIDEAQRLAGEGARELILLAQDSTAYRDGDADLAMLLKGLTRIPGV
                     EWVRLMYAYPGRIGDGLMDVIAGEEKVCKYLDIPIQHFDDKVLAAMNRRGSSADIRKT
                     IERLRERVPGIALRTSLIVGFPGETDAAFKRLLSFVKEAEFDHLGVFTYSPEEGTAAS
                     GLEPRVPADVAADRYHAIMKAQAKISLRKNRALIGATLRVLIDGMEDMALTGRLMSQA
                     PEIDGMVYLSETEAVAGEFVDVTITDATEYDMMGKAATISTK"
     gene            complement(1455..1913)
                     /locus_tag="VFK23_02690"
     CDS             complement(1455..1913)
                     /locus_tag="VFK23_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7318023.1"
                     /translation="MIDLLKKISLFENLTAAELGKIEKIVRKERYARDIAIFREGDPG
                     NRCYVITAGEVRISKFIPNIGEEALAVLKAGDYFGEMSLIDNFPRSAHAIANTDIEVL
                     AIGKADLDNVLITDRDLGYKLLWSFTRTLSKRLRETNEKMAGFLVLSGGF"
     gene            complement(2074..2448)
                     /locus_tag="VFK23_02695"
     CDS             complement(2074..2448)
                     /locus_tag="VFK23_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET7318024.1"
                     /translation="MNLFRKNIGQEGEDRAARYLVKLGYRILERNYRTPAGEIDLIAL
                     HQGTVVFVEVKTRTSDAYGAPELAVTPQKQRRMIKAALGYIKHRKFHQVPCRFDVVAI
                     SAATEKEVELIQNAFEMDHTRY"
     gene            complement(2484..>2840)
                     /locus_tag="VFK23_02700"
     CDS             complement(2484..>2840)
                     /locus_tag="VFK23_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET7318025.1"
                     /translation="ILQATIIAMERALELLAPKPDYLLLDALTLPRVPLPQKPLIKGD
                     CRSHSIAAASILAKVTRDRIMLELHEKYPLYHFQNHKGYGTAEHLALLREHGPCDAHR
                     KSFHPVAALLSRERIF"
BASE COUNT          515 a          849 c          848 g          628 t
ORIGIN      
        1 gtttggaatt tggttttttg atgcttgaca tttgtttgtt atttggtgct tgctatttgg
       61 tgcttatcgt tgccgccttt cccatcatat catattccgt cgcgtcggtg atggtgacgt
      121 cgacgaattc tccggccacc gcttcggtct cggaaaggta caccatgccg tcgatctccg
      181 gcgcctgcga catcagcctc cccgtgagcg ccatgtcctc catgccgtcg ataagcaccc
      241 gcagcgtggc gccgatcagg gcgcggttct tcctgagcga gatcttcgcc tgggccttca
      301 tgatcgcgtg gtatcggtcc gcggcaacgt ccgccgggac gcggggttcg agcccgctgg
      361 cggccgtccc ttcctcgggc gaataggtga acacgccgag gtggtcgaac tcggcttcct
      421 tgacgaaaga cagcagccgc ttgaacgccg cgtcggtctc cccggggaat cccacgatga
      481 gagaggtccg gagcgctatg cccggcacgc gctcccggag ccgctcgatc gtcttccgaa
      541 tgtccgcgga cgatccgcgc ctgttcatgg ccgcgagcac cttgtcgtcg aagtgctgga
      601 tcgggatgtc caggtacttg cagaccttct cctccccggc gatcacgtcc atgagaccgt
      661 cgccgatccg tccgggatag gcatacatga gcctcaccca ctctacgccc gggatccgcg
      721 tcaggccttt cagcagcatg gcgagatcgg catcgccgtc ccggtatgcc gtggagtcct
      781 gtgcgagcag tatcagttcc ctggccccct cgccggcgag cctttgcgcc tcgtcgatga
      841 cggacgcgga gggcctgctc ttgaaattcc cgcggatgag cgggatcgcg cagtaggaac
      901 accggttgct gcatccttcc gcgatcttga ggtaacggta gtgaaggggc gtagtcgaga
      961 ggcgcggaag gccgtagacc attgcgggcg gcgggaccct gagcagctgc tccttcccgg
     1021 aaagggcttc cccgcagatc tcggcgatcc tgccgacctc ggccgtgccg atgacggcgt
     1081 ccgcctcggg cagctcccgc aagagctcct cccggtaccg ctgggcaaga cagccggaga
     1141 cgactacctt cgcgcacctg ccctccgtct tcatccgggc ggcatcgagg accgcctcga
     1201 tggattcctc cttggcggac tcgatgaacc cgcaggtgtt tacgatgatg acgtttgcct
     1261 cggcttgatc cggcgtgatc tcgaagcccg atgccgccag cacaccgagc atctgttcgg
     1321 catccacctg gttcttgggg cagccgaggc tgaccatgcc tattttgtat gatttcttta
     1381 ccacggattt ctcaagaaaa cggattaacg gagtactata ccacttgacg aggacgctgg
     1441 caacagcgta tgcatcaaaa ccctccggac aggacaagga agccggccat cttttcgttc
     1501 gtttcgcgca gacgtttcga cagggtccgg gtgaacgacc agagcagctt gtatcccagg
     1561 tcccggtctg tgatcaggac attgtccagg tccgctttgc cgattgcgag aacctcgatg
     1621 tcagtgttgg cgatggcgtg ggcagagcgc ggaaagttgt cgatcagcga catctcgccg
     1681 aaatagtctc ccgccttgag cacggccagg gcctcctcgc cgatgttcgg gatgaatttg
     1741 ctgatgcgca cctcgcccgc ggtaatcacg tagcaccggt ttccgggatc gccctctcgg
     1801 aatatggcga tgtccctggc gtatctctcc ttcctgacga tcttctcgat ctttcccagc
     1861 tccgccgccg tgaggttctc gaacagggat attttcttca gcagatcgat catggagtct
     1921 ccgttctggc gctttgccac tgcgtctgtc attcccgccc cggcttaaat aatgctgggg
     1981 caggctccag cgggaatcca tttgggggga aaatggattc cgggtcaagc ccggaatgac
     2041 accaaaaggg gaaaagctct taggataact actttagtag cgcgtgtggt ccatttcgaa
     2101 ggcattctgg atgagttcca cttccttctc cgtcgcggcg ctgatggcca ccacgtcgaa
     2161 ccggcacgga acctgatgaa acttcctgtg tttgatatat ccgagagcgg ccttgatcat
     2221 gcgccgctgc ttctgcgggg tgaccgcaag ctccggcgcg ccataggcgt cgcttgttct
     2281 ggtcttgacc tccacgaaga ccaccgtccc ctggtgcagg gcgatcagat cgatctcccc
     2341 ggccggggtg cgataattcc gctccaggat cctgtagccc agttttacca ggtaccgggc
     2401 agcgcggtcc tcgccttcct gtccgatatt ttttctgaag agattcatat cacaaagatt
     2461 cgcacaacat ggttaaagga agatcagaaa atacgctccc ggctaagcag cgcagccacg
     2521 ggatggaagc tcttgcggtg cgcgtcacag ggcccgtgct cgcgaaggag cgcgaggtgc
     2581 tccgcggtcc catacccctt atgattttga aaatggtaga ggggatattt ctcgtggagc
     2641 tccagcatga tccggtccct ggtcaccttg gcaaggatgg aggcagcggc aatgctatga
     2701 ctccggcaat cgcctttgat gaggggcttt tgtggaagcg gaacgcgggg aagggtgaga
     2761 gcatcgagaa gaaggtagtc gggctttggc gcgagaagtt cgagcgcccg ttccatggcg
     2821 atgatggtcg cctgaagaat
//
LOCUS       DASSLD010000143         6537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1262265, whole genome shotgun sequence.
ACCESSION   DASSLD010000143 DASSLD010000000
VERSION     DASSLD010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262265"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1304)
                     /locus_tag="VFK23_02705"
     CDS             complement(81..1304)
                     /locus_tag="VFK23_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET7318026.1"
                     /translation="MSNIRMRLKRLLFSISSLIDLGQEATSAKDLSEKMKAALYVITG
                     TFSVPTAVLFVYNPQRKHLDFLAEKGYRDTDVRSLRLGVRPGDIKSFAMNSPHDVKDA
                     AKSSFFKVHEDLFTRLKIRMFIPLFAKGEFIGIILLGRKLGNASFRQGEKDVLTVIAH
                     QMAITLHHAQLFLELARKASENRRLYRNMHRIYHDTIQAFATAIDAKDGYTKDHSYRV
                     ASYAVAIAKELGWKKKDVEGIYIAGLLHDIGKIIIDTKVIRKGKGLTSYEMNEIRKHP
                     QISYDILSKVRFPWKNIDFFVRHHHERLDGKGYPDALVSEELSDGVKILALVDAFDAM
                     TTDRPYRQKLGLDETFREVIKCCGTQFDERITRTFFNLLLRELSGEVKDPQILPLLGQ
                     AQTVKLTAFRPRADV"
     gene            complement(1312..3267)
                     /locus_tag="VFK23_02710"
     CDS             complement(1312..3267)
                     /locus_tag="VFK23_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7318027.1"
                     /translation="MLFSQLQEKDFVDRREELDGLSARVLQADGGRARSVVLSGPRGI
                     GKTELLRHLFGRLFWKQDRVAPFQLTINPAVLSAAEFANAYLCRFLCQRLAFQKREQA
                     LLMSDGISLDALTSLVEEQEALWAKEILDEYVQNSGDPLDALQVALAAPRRSVVATGT
                     PVAVLIDDFHLLRGLHLSGDPDPRLVSLFEEPMSYGKTPHVITGNAAELQEMPVAGSL
                     ERMSLLPFSPEGVSSKVLSLLNNHEARTDAPPLLLRRLGGNPLYLDCVVTRACAANSP
                     DDKDFWNAYIREITEGTLSLSWTAAFKRAFPGLGMRRIAIEMAYKIYHSTEPLSSRRI
                     ARMFSLPESGAEDAAHALYLAGYIQGEFGVFRAVEDPVLKDIIDCLYRREVLGKSLPD
                     LEKDFLERLVPQKEHEVRFDITLPMAKDAELVAAQGLEQIGKSLGLSQDAVGQMQIAV
                     IEACINAMEHGRGTEGKVFVSISADDDRLEVSVESAGREFIIQETGEPFSDPGGRKTP
                     GRGWGIKLIKRFVDHIKFEKTSRGTKVVLVKKLAKSARDGIAVMMPRGYINDVGASRL
                     EQMREQLLNQGSKKLVVNFTDVQFINTIGLSIFLGIVQKTLEHRSSLCFTNMKKDHRE
                     IIEVAGLTKFIKIFKDENDAFHYLTGR"
     gene            complement(3404..5422)
                     /locus_tag="VFK23_02715"
     CDS             complement(3404..5422)
                     /locus_tag="VFK23_02715"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7318028.1"
                     /translation="MPDTITGGQLQDLFRTEMWRDHFTALSESLGFPLRIYSQSGALI
                     FAPPGPAPLCPAFRSPSSPFASRHVAECQPLAMNTLSTKKPDIFKCSANIMSFALPIE
                     YMDEKAVIFGQGSFSSYQDFREYLNIVNAEELETISLPAPTSFTSFEQVWKACVIVAD
                     SVKRLLQNTQETAALRTKFDRLKNIIGIWGALDGEQPEKLYNHMAGELASLLDIDRIS
                     VLIHDKKQGNYTNRFDLAAGESEICLSEQDSVVLGLIDGKSFVRTPGASGKRGAGHLK
                     GPGTRYFFSIMSSENLEGILVTAGRDLVEGDRQVISAFCRQAALYLENRRLQHKLARK
                     FERFAAISELTKSITPIQNYETLVRTILDMSAYLLKAERGSLMLIDQETDALLLEAKK
                     GMIDGVADKLRINRGEGIAGRVAAIGEPILVEDLESDPRIRQKNRGSYKTRSFVSVPL
                     KIDERIIGVLNLSDKMSGEVFNEDDLSLIQSFATQAAVVMERQVFINKTEELKKLTNT
                     DPLTGLPNRRYLFERLKNELARAERYNHPLSLLMLDLDGFKECNDSRGHLYGDGILRN
                     IADTLFQTVRSMDIVARYGGDEFIVILPETAEPLARDIAERVRGNIMRSVPDRHDAHD
                     AETHMLTASIGIVCYPDHGKTPEQLLENVDRALYRAKKLGKNRAEVYS"
     gene            complement(5748..6344)
                     /locus_tag="VFK23_02720"
     CDS             complement(5748..6344)
                     /locus_tag="VFK23_02720"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HET7318029.1"
                     /translation="MRKMMIPVMVVAMIVSCAALVSARQGAPAERSDETAEFDNPQAP
                     GGELSEERREEVRKKIEAVRIWRLTEALKLDAPTSSKLSALLSSFDQQRRDIMREQMT
                     TMKQLRHLLDSPKPDESKIKTALDRLEKNRHAMEALRDKEIGGVKDILTIEQQARFLL
                     FQQEFRREMQGMIANARKGGPGPRGGSRGEGPGRSQDR"
     gene            complement(6347..>6537)
                     /locus_tag="VFK23_02725"
     CDS             complement(6347..>6537)
                     /locus_tag="VFK23_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318030.1"
                     /translation="LDPDELDTAETWAGNELASISREVEQGVVNGADTDLYEELRQLN
                     AGEVERLSTMVEQWKQEG"
BASE COUNT         1296 a         1841 c         1837 g         1563 t
ORIGIN      
        1 tcaatatgaa gtgaagataa tgacagtccg gggcgttgtg aggccggggg cgggaaaaat
       61 tcggaacagg ggggtgaaaa ctacacgtct gcccttggac gaaaagccgt gagcttgact
      121 gtctgcgcct gtcccagaag ggggagaatc tgagggtcct tgacttcgcc gctcagttcc
      181 cggagcagca ggttgaaaaa cgttctcgtg atccgctcgt cgaattgcgt gccgcagcat
      241 ttaatgactt ccctgaatgt ttcatcgagc cccagcttct ggcggtacgg gcggtccgta
      301 gtcatggcgt cgaaggcatc gacgagcgcc aggatcttga ctccgtcact tagttcctcc
      361 gagacgagtg cgtcgggata tcccttccca tccaggcgtt catggtggtg gcggacaaag
      421 aagtctatat tcttccaggg gaacctgacc ttcgagagga tatcgtacga gatctgcgga
      481 tgttttctga tttcgttcat ctcgtatgac gtaagtccct tgcctttcct tatgaccttc
      541 gtgtcgatga tgatcttccc gatatcatga agcagaccgg cgatatagat gccctccacg
      601 tccttcttct tccagccgag ttccttcgcg atcgctacgg catagcttgc cacgcggtag
      661 gagtggtcct tggtgtagcc gtccttggcg tcgatggcgg tagcaaatgc ctggatcgtg
      721 tcatggtaga tccggtgcat gttcctgtag agtctcctgt tttccgaggc cttccgcgca
      781 agctccagga aaagctgcgc atgatgcagc gtgatcgcca tttgatgcgc gatcaccgta
      841 agaacgtcct tttccccttg gcggaatgac gcattgccga gtttcctgcc cagaagaatg
      901 atgccgataa attccccttt ggcgaacagc gggatgaaca tccgtatttt aagccgggtg
      961 aacagatctt catgaacctt gaaaaacgaa ctctttgctg cgtctttcac atcgtggggg
     1021 gaattcatcg cgaaagactt tatgtcgccg ggccgtacgc ccagacggag cgaccggaca
     1081 tcggtgtccc ggtaaccctt ttccgcgagg aagtcgaggt gtttccgctg ggggttgtat
     1141 acgaacagga cggcggtcgg cacggagaat gtcccggtta tgacatagag cgccgctttc
     1201 attttttccg acaggtcctt cgcggacgtt gcctcctgcc ccagatcgat cagggacgag
     1261 atgctgaaca gcagccgttt gagacgcatt ctgatgtttg acatcctgag ttcatctccc
     1321 ggtcaagtaa tgaaaagcgt cgttttcatc cttgaagatc ttgatgaatt ttgtgaggcc
     1381 cgccacctcg attatctcgc gatggtcctt cttcatgttc gtaaaacaga gcgagctcct
     1441 gtgttccagc gttttctgca cgatgcccaa aaaaatagag agcccgatgg tgttgataaa
     1501 ttggacgtcg gtaaaattca cgacgagctt tttcgatccc tggttgagta actgttctct
     1561 catctgctcg agtctcgaag caccgacgtc attgatgtac cctctgggca tcatgacggc
     1621 gatgccgtct cgcgcggact ttgccagttt cttgacgagt acgacctttg tcccgcgcga
     1681 cgttttttcg aatttaatgt gatcgacgaa gcgtttgatc aatttaattc cccatcctct
     1741 tcccggtgtt tttctcccgc ccgggtcgct gaagggctct cccgtctcct ggatgatgaa
     1801 ttcacgcccg gcgctttcaa ccgacacctc cagacggtcg tcgtcggcgg agatgctgac
     1861 gaagaccttt ccttccgttc cccttccgtg ttccatcgca ttgatgcagg cttcaatgac
     1921 ggctatctgc atctggccga ctgcatcctg gctgaggccc aggcttttcc cgatctgttc
     1981 gaggccctgc gccgcaacga gctcggcgtc cttggccatc ggcagggtga tgtcgaagcg
     2041 gacctcatgc tctttttgcg ggaccagcct ttccaggaag tccttctcca ggtccggaag
     2101 ggattttccc aggacttctc tccggtagag gcagtcgatg atgtccttca acaccggatc
     2161 ttcaaccgcc ctgaagacgc cgaactctcc ttggatgtaa cccgcgagat acagggcatg
     2221 cgccgcgtcc tcggcaccgc tttccggcag cgaaaacatt cgcgcgatcc ggcggctgga
     2281 aagcggctcg gttgaatgat aaattttgta ggccatttcg atggcgatcc gccgcatccc
     2341 caggcccggg aaagcgcgct tgaaggcggc cgtccaggag agggagagcg tcccttccgt
     2401 gatctcccgg atgtaggcat tccaaaaatc cttatcgtcg gggctgttcg ccgcgcaggc
     2461 gcgggtgacg acgcagtcca ggtacagggg gttgccgccc aggcggcgga gcagcagcgg
     2521 aggagcgtcg gtccgggctt catggttgtt caggagagag agcaccttgg aagaaacccc
     2581 ctccgggctg aaaggcagga gggacatgcg ctcaaggctt cccgcgacgg gcatctcctg
     2641 aagctcggcc gcattccccg tgatcacatg gggggttttc ccgtaggaca tcggttcctc
     2701 gaacagcgac accagtcggg gatcggggtc accgctcaga tgaaggcctc tcagcaggtg
     2761 aaaatcatcg atcaggaccg cgacgggagt ccccgtagca acgactgagc ggcggggcgc
     2821 cgcaagggca acttggaggg cgtccagggg atctccggaa ttttgcacgt actcgtcgag
     2881 aatctccttt gcccagaggg cctcctgctc ctcgacgagg gaggtaagtg catcgaggga
     2941 aatcccgtcg ctcatgagca gggcctgctc acgtttctgg aaggcgagcc gctggcagag
     3001 aaaccgacag agataggcgt tggcaaactc cgccgccgac agcaccgcgg gattgatagt
     3061 gagttggaag ggggcgacac ggtcctgctt ccagaagagc cggccgaaca ggtgtctgag
     3121 caactccgtt ttcccgatgc cgcgagggcc ggacagcacg acgcttcgcg cacgcccccc
     3181 gtccgcctgg aggacccgtg cggagagtcc gtccagttct tcccgcctgt cgacaaaatc
     3241 tttttcctgg agttggctga acagcatagt atgctatgtc ttccccatat ttgatatgga
     3301 tgcgtgaatc atatcgttgc gagttgcgcg atacgttacg ggttaaaccc gcaacgcatg
     3361 acctgcaacc tataccagct cttggtttcg gccggaggtc gagctatgaa tagacctctg
     3421 ccctgttctt ccccaacttc ttggctcggt acagcgcccg gtcgacgttc tcgagaagct
     3481 gttcgggcgt tttcccgtga tcgggatagc agacgatgcc gatgctggcc gtcagcatat
     3541 gcgtttccgc gtcgtgagca tcatgacggt ccggcaccga ccgcatgata ttgccccgga
     3601 cccgctccgc gatgtctctt gccagcggtt ctgcggtttc gggcaggatt acgatgaatt
     3661 catcgcctcc gtatcgcgcc acgatgtcca tggatctcac tgtctgaaac agggtgtccg
     3721 ctatgtttct caatatcccg tccccgtaaa gatggccccg ggaatcgttg cactccttga
     3781 atccgtcgag atcaagcatg agcagactga gggggtggtt gtaccgttcg gccctggcta
     3841 attcgttttt cagccgttcg aaaagatacc ggcggttcgg gaggcccgtc agggggtccg
     3901 tattggtcag ttttttgagc tcctcggttt tattgataaa cacctgccgt tccatgacga
     3961 cggccgcctg agtggcaaag gactgaatga gagacaggtc gtcttcattg aagacctctc
     4021 cggacatctt gtcagaaagg ttaaggacgc cgatgattcg ttcatcgatc ttgagcggga
     4081 cgctgacaaa ggaacgcgtc ttgtagctcc ctctgttttt ctgcctgatc ctcggatcgc
     4141 tttcaaggtc ttcgacaagg atgggttcgc cgattgcggc cactctgccg gcgatcccct
     4201 cgccccggtt gattctgagc ttgtccgcga cgccgtcgat catgcctttt ttcgcctcga
     4261 ggagcagggc gtccgtttcc tggtcgatca gcatcagaga gccccgttcc gccttgagca
     4321 gataggccga catgtcgagg atcgtcctga ccagcgtttc ataattctga ataggtgtta
     4381 tcgatttcgt cagctccgag atcgcggcga aacgttcgaa cttccttgcg agtttgtgct
     4441 gcaggcgccg gttctcaagg taaagggcgg cttgccggca gaacgcgctg atgacctgtc
     4501 tgtccccctc cacaagatcc ctgccggcgg tgaccagaat gccttcaagg ttttcactgc
     4561 tcatgatgga gaaaaaatag cgggtcccgg ggccctttag gtgaccggcg ccgcgttttc
     4621 ccgacgcccc cggggtgcgg acaaacgact ttccgtcgat caggcccagg acgactgaat
     4681 cttgttcact gaggcaaatc tcactctccc cggctgcgag atcgaaacga ttcgtataat
     4741 tgccctgttt tttatcgtgg atgagaacag aaatacggtc gatatcgagc agggaggcca
     4801 gttcgccagc catatggttg tagagttttt cgggttgctc tccatcgagc gccccccata
     4861 ttccgatgat gtttttcaga cgatcgaact ttgtcctcag ggctgccgtt tcctgggtgt
     4921 tttgcagcag tctcttcaca gagtccgcga caataacgca tgctttccaa acctgctcga
     4981 agctcgtaaa ggaggtaggg gcgggcaggg aaatggtttc aagctcctcc gcattcacga
     5041 tgttcaggta ttcgcggaag tcctggtaag aggaaaaaga tccctgcccg aagatgaccg
     5101 ctttctcgtc catgtactcg atcggaaggg cgaaactcat gatgtttgcg ctgcacttga
     5161 agatatccgg tttttttgtc gagagcgtgt tcatcgcaag aggttgacat tctgcgacgt
     5221 gacgcgaggc gaatggggat gaaggcgagc ggaacgccgg gcacaggggc gccggtcccg
     5281 gcggagcaaa gatcagcgca cccgactgtg aataaatgcg caggggaaaa ccgaggctct
     5341 cggacagggc cgtgaagtgg tcccgccaca tttcggtcct gaagaggtcc tgcaattgtc
     5401 caccggtaat cgtgtccggc acaaagcctc cgtgcgacac atctgctcaa gcgaagaacc
     5461 ctgtcaaacg gagagggtat cgctaacggg gcttagttta acatacgtca gtccgaatga
     5521 aaaggatttt ttttaatttt gtttttgtaa tacccaggtt tatttgtcga aatgccatgc
     5581 cagaatgtta caggaggtag cacaattcca tcttcctctg cgttctcaag cgagcaacag
     5641 ggaaaaacag gcattctttt acgaaaaaac gggtccctaa gaaaaaacta agtggacgac
     5701 tcatttttta ttgggcgtca tcatcacagc gcggcatgtt atctaaatca acgatcctga
     5761 gaacgacccg gtccttctcc ccgggagccg ccacgcggcc cgggtccgcc tttgcgggca
     5821 ttggcgatca tgccctgcat ctcgcgccgg aactcctgct ggaaaagcag gaaacgcgcc
     5881 tgctgttcga tcgtcagaat atccttcacg ccgccgatct ctttgtctct cagcgcctcc
     5941 atggcatgcc ggttcttttc cagcctgtcg agggcggtct tgattttcga ctcgtccggt
     6001 ttcggcgagt cgagcaggtg ccggagctgt ttcatggtcg tcatctgctc gcgcatgata
     6061 tcccttctct gctgatcgaa ggagctgagc agggcggaca gcttcgagct tgtcggggca
     6121 tcgagcttga gcgcttccgt gaggcgccag atcctgaccg cttcgatttt cttccttact
     6181 tcttcccgcc tttcctccga aagttctcct ccgggagcct gggggttgtc aaattccgcc
     6241 gtctcgtcag agcgctccgc gggtgctccc tgacgggcgg acacgagcgc cgcgcatgag
     6301 acaatcatcg ccacgaccat caccgggatc atcatctttc tcatcgtcat ccctcctgtt
     6361 tccattgttc gaccatcgtc gaaagccgct ccacttcccc cgcgtttaat tgtctcaatt
     6421 cctcatagag gtccgtgtcc gcgccgttca ccacgccctg ttcgacctcg cgggagatcg
     6481 aagcaagctc attgccggcc caggtctccg ccgtgtcgag ttcgtcagga tcaagct
//
LOCUS       DASSLD010000144         6019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_867037,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000144 DASSLD010000000
VERSION     DASSLD010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867037"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            430..1266
                     /locus_tag="VFK23_02730"
     CDS             430..1266
                     /locus_tag="VFK23_02730"
                     /inference="COORDINATES: protein motif:HMM:NF014078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HET7318031.1"
                     /translation="MHRSNAKAGLSQAAGPSYVPVAGKTYSLFGNDLSTEPYYDRVRQ
                     LADDLLRIVPGEESLLALIRKAGGRKWLLDRAARREDRSILSSILRVLGNALSPHTAN
                     VRTHLKGLSIRSVTDRRLRTTEEQYHLHMLEIELVNRVHRDRFLRSDFRIALLPHCLR
                     DFRRECRSVQGDLDSVCAGCSETCFLHRVSAILREAGIHPYIWRNAELSTLFRRLEGR
                     GSFGVLGIACIPELAWGMRACMKRKIPVVGIPIDANRCMRWMGRFDETSVNLDRLSEL
                     VN"
     gene            1988..2188
                     /locus_tag="VFK23_02735"
     CDS             1988..2188
                     /locus_tag="VFK23_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318032.1"
                     /translation="MSWLLIVGPVTAGALLTAAKGALDRAGAEKGSAIIARATFCTVV
                     AVGFGIWVAIPWIDILVHSLRN"
     gene            2319..2768
                     /locus_tag="VFK23_02740"
     CDS             2319..2768
                     /locus_tag="VFK23_02740"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7318033.1"
                     /translation="MDKPFVAENARERKRLTALVKQLTDEELQIPLEAGRTIAAVLAH
                     LAFWDQWSLMRLRRWKQRGVVASSVDLDAVNDTFLPFSLALEPRVAAQMAVSSADVID
                     RELEEISDELIRAIESLGDRFRLYRSIHRKMHLDEIEAALRSKRGTT"
     gene            2856..3533
                     /locus_tag="VFK23_02745"
     CDS             2856..3533
                     /locus_tag="VFK23_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7318034.1"
                     /translation="MAVDIFPISLGFDQCYVLKSEGVIAIDAGFPGKGEHFAQGLKRA
                     SINSHDVQLIVLTHGHWDHIGSAKDIKNITGGKLAMHHREASWLERSIKPLPPGITLW
                     GKVFIGIHKLFMPLITIAPVEVDVLLGDEGLSLADYGIPGKILFTPGHSSGSVSVLLE
                     TGEAFVGDLAMNRFPLRLSPGPAILGDEPDKISVSWKLLLEHGATTVYPAHGKPFPAD
                     VIRKALR"
     gene            complement(3762..4355)
                     /locus_tag="VFK23_02750"
     CDS             complement(3762..4355)
                     /locus_tag="VFK23_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET7318035.1"
                     /translation="MTRNPSREEAWTILTDHIKSPGLISHALAVEAVMRYIARKRGAD
                     EELWGVIGLIHDVDYEEHPDEHLRHATEILRRHGWPEECVRAVASHGWGLFTDVAPVT
                     DLEKTLFAVDELTGLIAAATLVRPSRSVLDLPVKSVLKKWKDKGFAAGVDRKVIEKGA
                     ALMEVPLDQLIADTIEGMKTVAETIGLKGNPDLSPSS"
     gene            complement(5157..5540)
                     /locus_tag="VFK23_02755"
     CDS             complement(5157..5540)
                     /locus_tag="VFK23_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318036.1"
                     /translation="MKTKGKIGGAVMLEEFRGWIHRHFNVVLSFVVGLTSGISILALV
                     YFSTRNDIAARALRYGVQITGDIDIAEDLLWFICGIVVGASVICLAWWRDSKAQLVGL
                     EKADVLNRILTDPKPPIVRKEEKEK"
BASE COUNT         1300 a         1590 c         1659 g         1470 t
ORIGIN      
        1 atctatgaga cccggtgggt gttttatttc tgaaaaatga tgctcaaatc ttgtgtccaa
       61 attgtgacca cgagaattca cgttcattca cagtccttca taacctatca aaaacattga
      121 gaaataattt tggtccaaat cctcaatgca aatctatttc ctttttataa tcaataggtt
      181 acgtgcaatt tttcgcgcct aataacccga aggtcattac gctgaattat actgttgggc
      241 gcgcaccccg aagatctatc tcttcaagtt gtcttactgg aaagagtatc cgatccaccg
      301 cactggcttg tggccgtctt gtgacaggat tacggaccct ttaggaaatc cccgtttggt
      361 tacatgcccc attctcgagg gcgccaaagg gtgctccgtg ggtcgatctg cggaggtata
      421 atgtaaaata tgcatcgttc gaatgcgaag gcagggttga gccaagctgc cggtccctca
      481 tatgtaccag tcgcaggtaa aacctactcg ctgttcggaa acgacctctc gacagaaccg
      541 tattacgatc gggttcgcca gcttgcggat gacctccttc gaatcgttcc aggagaggag
      601 tcactgctcg ccctgatccg gaaagccggc gggaggaaat ggcttctcga cagggcggcg
      661 cgacgggagg acagatcgat actttcctcc atcctccgag tgctgggcaa tgccctctct
      721 ccacatacgg caaacgtacg aacacacctg aaagggctgt cgatccgatc cgtgacggac
      781 cggaggctcc ggacgaccga agagcagtac cacctgcaca tgcttgagat cgaactggtg
      841 aaccgggtcc acagggaccg cttcctgcgt agcgatttcc ggattgcgct gctgcctcac
      901 tgtctccggg acttccggcg agagtgccga tcggtccagg gcgatctcga ttccgtctgt
      961 gcggggtgtt cggagacctg ctttctccat cgcgtcagcg caatcctgcg ggaagccggg
     1021 atccacccct acatttggag gaacgcggag ctctcaaccc ttttccgtag gctcgagggg
     1081 agggggtcct tcggtgtcct ggggatcgcc tgcatcccgg aattggcatg ggggatgcgc
     1141 gcctgcatga aacggaagat cccggtagtc ggaattccga tcgatgcgaa ccggtgtatg
     1201 cgctggatgg gccggttcga cgaaacctcc gtcaatctgg accgcctgag cgaactcgta
     1261 aattgatgcc gcatctcgac aacgcgctgc agtcgacacc gacaagccgc gcctctgagc
     1321 gtgagcgttg ctagttgggt atcagatttc accttcgggc acccaccccg aaggtcgatc
     1381 cgccgaagct atcattgttg aaaatgtatc cgatacccag tttgcgcata tgaccgtgat
     1441 gtgaccgagt taaagccttc ttggtacgct aagatctaat cgttcatttg gtaaaatacc
     1501 tacttacagg tcacggtcct cctgactttc tgctaccagt ttggaccttc gggtaggtaa
     1561 tagcatgatc ttcgaattac cagttaggtc cgccagcaca tcgtctaatt attgtttggc
     1621 gaatgaaata gagcgaaggg ctccaatctg aagcggagcg ttgatcgtga gaattttgac
     1681 ccgtagacag ctgtatgtcg gccttgttat gtccaatggt ctgctgttcg caattactct
     1741 ttgcttcact cccctaacta cagcgctcgg cgtcccttgg gttggctaca cctgggatga
     1801 cagtcatagg ttcgttattc cacacctggt gttgtcaggc cttctcttgg cgttctacgg
     1861 cttcggtgtc acgggtagtg tagacgatcg ccaagtcctg cgggtaagcg gttgggtgca
     1921 gccactgtca gttggaacct acttttgggc aatgctacag tggcccggtg gcgacgacgg
     1981 cggtggcatg tcttggctct taatcgtggg accagtgacc gcaggggctc tcctcaccgc
     2041 ggccaaaggt gcgttggata gagccggcgc cgagaaggga tcagccatca tcgcccgtgc
     2101 cacgttttgc acagtggttg ccgttgggtt cggcatctgg gttgcgatcc cctggatcga
     2161 cattctggtt cattcgttgc ggaattaaag tgcactgtgg cccaacaaat ggctgcaccc
     2221 gaccgtccgg ctgaagctga cggctcacct gggggacttc gtgagtgccc gcagcttagg
     2281 ctgagcgtta ggttggtgct cacgtcaaag gaggcttgat ggataagccg tttgttgcgg
     2341 agaacgcgcg tgagcgtaag agacttaccg ctctcgtcaa acaattgacc gatgaggaac
     2401 tacaaattcc ccttgaggcc ggaaggacaa tcgctgccgt ccttgctcat cttgcttttt
     2461 gggatcagtg gtcgctgatg cgcctgcggc ggtggaaaca acgcggcgtg gtggcatctt
     2521 cagtcgatct cgacgctgta aacgatacct ttctcccctt tagccttgct ttggagccaa
     2581 gggtggcagc gcagatggcg gtttcttcgg ctgatgtgat cgaccgcgaa ctggaggaga
     2641 tttccgatga attgattcgc gccatcgaaa gccttggaga taggtttcga ttgtaccggt
     2701 caattcacag aaaaatgcac ttggatgaga tcgaagctgc ccttcggagt aaacgaggca
     2761 caacctaact accggctgaa gctgatggct cgcctgattc tcgctgagcg cctgcagctt
     2821 agccagagcg ttagccgctc gaaaagagcc ttctgatggc agtggacata ttccccatta
     2881 gtcttggctt cgaccagtgc tacgtcctca agtcggaggg tgtgatagcg atagatgcag
     2941 gttttcctgg aaagggagag cacttcgcgc aaggacttaa gagggcatcc atcaactcgc
     3001 atgatgtcca attgattgtc cttacccacg ggcactggga ccatatcggg tccgcaaaag
     3061 atatcaaaaa catcaccggt ggaaaactcg ccatgcacca ccgggaggca agttggcttg
     3121 agcgatccat aaaacctctt ccccctggga ttactttgtg gggaaaagtg tttatcggaa
     3181 ttcacaaatt gtttatgccg ctgataacca ttgcacccgt cgaggtcgat gtgttgttgg
     3241 gcgatgaggg actgtctttg gccgactacg ggatcccggg caaaatcctt tttacaccag
     3301 gtcactcatc gggctccgta agtgtcctgc tggaaactgg tgaagcgttt gttggagacc
     3361 ttgccatgaa ccggtttcct ctccgtctct cccccggacc tgccatcttg ggtgatgaac
     3421 ccgataagat ttcggtgagc tggaagcttc tattggaaca cggcgcaaca actgtttacc
     3481 ctgctcacgg caaacctttt ccggctgatg tgatccgaaa ggctctccga tgacggggca
     3541 cctattcact caaattccgg atcccatggt ccgtccgggt gaccacgccg atgtaggtaa
     3601 cggaatgaat aagagattgg agggagaccg acaggatcag atgaataagg cgaaacaaaa
     3661 gatgttggag aataaaggat gcgttcgtca ggggacgtat cggatcgtct atgcgatttc
     3721 cgatgtgggt ttaattgtct gtatggcaag catggcgagt ctcaggagga tggcgagagg
     3781 tccgggttgc ccttgaggcc gatcgtttcc gcgacggtct tcatcccctc gatggtgtcc
     3841 gcgatcagtt gatcgagcgg tacctccatc agggccgcgc ctttctcaat caccttccgg
     3901 tctacacccg cggcaaaccc cttgtccttc catttcttca ggacgctctt gacgggcagg
     3961 tccaggacgc ttcgcgacgg ccggacgagc gtcgccgccg cgatcagccc cgtcagttca
     4021 tcgaccgcga aaagagtctt ctccaggtcg gtgaccggcg cgacgtccgt gaacaatccc
     4081 catccgtggg acgcgacggc ccgtacgcac tcttcgggcc acccgtggcg ccgtaagatc
     4141 tccgttgcgt gccggaggtg ctcatcgggg tgttcttcgt agtccacgtc gtggatcaat
     4201 ccgatgacgc cccacagttc ctcgtccgcg cctctcttac gggcgatata gcgcatgacg
     4261 gcctcgaccg ccaaggcgtg cgaaatgagg ccggggcttt ttatgtgatc cgtgaggatc
     4321 gtccatgctt cttcgcgcga cggattgcgg gtcatattat ttcccatctt atctgcaacc
     4381 attggatttg acaaagcaac aaattgctga attcgagcca gtcgcgggtt gccaagatgg
     4441 aagctggtga tccaactgca tcccggcaac tttgcggatc gggggagggg tggaccttcg
     4501 ggtaactaat tgtattgatt tgattataag aaggaaccgc ccctacattg acgccctgca
     4561 taggcggttt atcacaccac cttatcacac ccgaacaatc ccccgctggg gcccgtcata
     4621 tccgggcttt ttatcacgcg ataggaggtc ggcacaattt ctgcggaaca aacacctcac
     4681 tccttccccc attgtcgaaa cggttttttg cccattttat tcgctaatat cgccttcctc
     4741 ataagttcat gcgggagatg atggctgatc tttactgcac gggtgttccc gcatcggcaa
     4801 ttccatataa ctgccttgtc cgcgtgaata ccgattggat ggacagtttc caggtcgtct
     4861 acttttccgc aaatcgcgca gggaagggat ggtgtcatga cgtcacctct tggccttcct
     4921 tgatgtcggg aattttattc cggcccctgt gcggggatac ctattagacg cgcaaggggg
     4981 atattgtgtt ccttccgtac catgaaaaag ggccctgtgg gagtaatcat tacccgcgcc
     5041 gggagcgttt gcggacgggg ggaatctggg ggagttcgtc gtcggggagg gcaggttatt
     5101 cctttccgtg agtacgaaga tgtaccgccc gagccgttca cgccactatc ggctgttcac
     5161 ttctccttct cctccttccg aacaatcgga ggctttggat ccgtcaaaat acggttcaga
     5221 acgtctgcct tctccaggcc gaccaattgc gccttgctat cccgccacca agccaggcaa
     5281 atgactgagg cgccgacaac gatcccgcag ataaaccaga gcaggtcctc ggctatgtca
     5341 atgtccccgg taatttgaac accatatcga agcgctctgg cggcaatatc gttcctggtg
     5401 gagaaataca caagtgcgag gatggatatt ccagaagtta aaccgaccac gaaggacagg
     5461 acaacgttga agtggcgatg gatccaaccg cgaaactcct ccagcatcac ggcacctcca
     5521 atctttccct tcgttttcaa gtttcccgaa aggagggagt attgccggtc tgcgtctcgt
     5581 tgtgatcgtg cctgccgggg acttccttga cagattttgg ggggaatgag gggtaccctc
     5641 cttatcgcaa ggaagccctc aaccggcagg ctacctatta gatgccacgc gttccgggct
     5701 gattccggtg ggtgttggac tctgtatgtg ctgcgccacg cctctgttta ggaacacaca
     5761 gaaaaatcaa tgagcagaga aggtgataat tggattgttg aaaattcgct ggggtcgatg
     5821 aaagtcagaa ttgtagaaaa taataaattc ggtgtattgg accaatatgt aacaacgcct
     5881 tcaggtgttg aagtctatgt tcccccgcgg tttgggaaga tcactttcgg ataagtcagc
     5941 tggtgagttt gccgatcagc agatgcgtga tctgcgagga aggaattctg gcatattcgt
     6001 tgtacagatt cgccttttt
//
LOCUS       DASSLD010000145         2415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1421565, whole genome shotgun sequence.
ACCESSION   DASSLD010000145 DASSLD010000000
VERSION     DASSLD010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421565"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1412)
                     /locus_tag="VFK23_02760"
     CDS             complement(222..1412)
                     /locus_tag="VFK23_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7318037.1"
                     /translation="MARIVILGGSFGGLTAAFELKRLLGKKADVTVVSDDELFVFLPS
                     LPWLIMGWRRPEDITLTVSEILRPKGIEFVHEAASQIDAAGSKVITSKGREVPYDHLV
                     ISTGPHLSFDEIPGLGPDMGYTACTFTLDHAMKTGETWKQVLENPGPIVIGSAQMVSC
                     FGPSYELAFEMDAELRCRRMRHKVPILYITSEPYVGHMGVGGLGNSKTFMEHEFAERD
                     IKPLVSQTIQEVVPGEIRLKDGNKIPFKLAMIAPPFKGVPVVQPLGNPRGFIPVDRNY
                     RHTKHKNIFAIGVAMAIAPPEATPVPTGVPKTGFMTVKMAKTAAATIASDVTGKTPPV
                     ADELGVVCLMDMGNTAAMMKAYPVLPPRQSSFMKKTIWAKWLKVQFEKYFLWKMKRGL
                     SYLP"
     gene            complement(1511..1792)
                     /locus_tag="VFK23_02765"
     CDS             complement(1511..1792)
                     /locus_tag="VFK23_02765"
                     /inference="COORDINATES: protein motif:HMM:NF016003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsrH/TusB family sulfur metabolism protein"
                     /protein_id="HET7318038.1"
                     /translation="MIVMIKSAPDTSDGAIGLTLAKEGGVDLVLLQNGVYYAQKDRLG
                     DFRGTVYILDDDKRLRGLKDSEMDARVKTIGYDALIDILTGGEKVVGMF"
     gene            complement(1803..2156)
                     /locus_tag="VFK23_02770"
     CDS             complement(1803..2156)
                     /locus_tag="VFK23_02770"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HET7318039.1"
                     /translation="MATIAMILKRSPYGDINAAEAVRHALGAVSYEMDIDLILMDGGV
                     LLAKKGQDDAGTGFTNLEAVLKDCLDMGVPVYADDISLKMQSIGREDLVEGVRAASSK
                     EIAGMVKEAKTTMIF"
BASE COUNT          482 a          767 c          590 g          576 t
ORIGIN      
        1 cagaagaccg aagctgctct ttttcacgat ttccgcttca aaagtgtaag ttttcttcag
       61 ctccgcgacc gcggcgctga ttttgtttca ccggcccccc agcgggtcgt taaagtagag
      121 acggaccagc attgttcttc ctccttttgc cggattaccc ggcggctaac atatcaacaa
      181 agagtcaggt ccatatgggc agggctccta gagccttctt ttcatggcag atagctgagc
      241 ccccttttca tcttccagag gaagtatttc tcaaactgca ccttcagcca tttggcccag
      301 atcgtcttct tcatgaaaga actctgcctc ggcggaagga cgggataggc cttcatcatc
      361 gcggctgtat tccccatgtc catgagacag acaacgccga gttcgtccgc gaccggtggc
      421 gtcttgcccg tgacatcaga ggcaatggtc gccgcagccg ttttcgccat cttgactgtc
      481 atgaacccgg tcttggggac ccccgtcggc accggcgttg cctccggggg ggcaatcgcc
      541 atggcgacac cgatcgcaaa gatattcttg tgtttcgtgt gccggtagtt cctgtcaact
      601 ggaataaacc ctcgtggatt tccaagcggc tggaccacgg gcactccttt aaaaggcggc
      661 gcgatcatcg cgagcttgaa ggggattttg ttcccatcct tgagccgtat ctccccggga
      721 accacctcct ggatggtctg gctcacaagc ggcttgatgt cccgctccgc gaattcgtgc
      781 tccatgaagg tcttcgagtt cccgagtccg cccacgccca tgtggccgac atagggctcc
      841 gaagtgatat agagaattgg caccttatgt cgcatcctcc tgcaccgaag ctccgcgtcc
      901 atctcgaagg caagctcgta ggacggtccg aagcagctca ccatttgcgc ggacccgatg
      961 acgatcggtc cgggattttc caggacctgt ttccatgttt ccccggtctt catcgcatga
     1021 tcgagagtga aggtacaggc tgtatatccc atgtccggtc cgaggcctgg aatctcgtcg
     1081 aaagaaaggt gcgggccggt cgatatgaca agatgatcgt agggaacttc ccttcccttg
     1141 gaagtgatga cctttgatcc agcggcatca atctgacttg ccgcttcatg gacaaactca
     1201 atccccttcg gcctcaggat ctcgctcacc gtgagcgtaa tatcctcggg ccttctccaa
     1261 cccatgatga gccagggcaa agacggcagg aacacgaaca gctcgtcgtc gctcaccacg
     1321 gtcacatccg cctttttacc gagcagccgc ttgagttcaa aggccgctgt caatcctccg
     1381 aacgatccgc ctaatataac gatccgagcc atgactacct cccctgtttc agacgcgcag
     1441 agggggagac ggggtgactc ggcgagacca catcgacaat caccgcgtcc ccgtgtcccc
     1501 gcgtcggcca ttaaaacatc cccaccacct tctcgccccc tgtgaggata tcgatcaacg
     1561 cgtcgtaacc tatggttttt acccgcgcgt ccatctcgga atccttgaga ccccggagcc
     1621 tcttgtcgtc atcgagaatg taaactgtcc cgcgaaaatc tccaagccgg tccttctgtg
     1681 cgtagtagac gccgttctgc aacagaacaa ggtcgacccc tccctctttc gcgagggtca
     1741 agccgatagc gccatcggac gtgtcgggcg cgctctttat catcacgatc atggcgacct
     1801 cctcaaaata tcatcgtagt cttcgcttct ttcaccatcc ccgcgatctc tttgctgctg
     1861 gccgcccgta cgccttccac gaggtcttcc ctacctattg attgcatttt cagggatatg
     1921 tcgtccgcgt agaccggaac gcccatgtcc agacaatcct tgagcacagc ctcgagattc
     1981 gtgaacccgg tccctgcatc atcctgcccc ttcttcgcga gcagcacacc tccgtccatc
     2041 aggatcagat caatgtccat ttcgtatgaa accgcaccga gggcgtgccg cacggcctcg
     2101 gcagcattaa tatcgccgta gggagacctc ttcaggatca ttgctatggt agccattttc
     2161 ccctctgctc tccttttatt ccctattccg caatcggcgt tgtttcttac gctccgacgt
     2221 tgatccaaac gtcgctagcg ctcattgttt cgaacacccc tttgagcgag ccgacctcgg
     2281 tgcctgccag ataatcgtcc ttcgccgcac ggcgaaagtt gagacacccg cctcaaaggt
     2341 agaccttgag ccccttctgg ataagccgcg tgaactcctc ctcggcgttc aggattccct
     2401 tcgccttctg ctttg
//
LOCUS       DASSLD010000146         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_964778,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000146 DASSLD010000000
VERSION     DASSLD010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964778"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VFK23_02775"
     CDS             <1..706
                     /locus_tag="VFK23_02775"
                     /inference="COORDINATES: protein motif:HMM:NF022800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HET7318040.1"
                     /translation="PAESGRAVLQPRRELSDYSSILERGLFGEGKAPSSGPATATSAT
                     SYKLIGTVEGAVFSGAVLEDGTGQAFYRTNQKLPDGSVIVKVMRDKISLKRPDGVTEN
                     LEVVDETKIVPMKAAGTASEGIKKLSGGKFMVDQKEVLASTENMSQILTQARAVPYTE
                     QGKTVGFKLSEIVPGSIYEKIGLVNGDVIQRVNSQEVDDPAKFFQLYQGLRNERNITI
                     DLIRGGQRQTLNYEIR"
     gene            889..>2375
                     /locus_tag="VFK23_02780"
     CDS             889..>2375
                     /locus_tag="VFK23_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HET7318041.1"
                     /translation="MKNKAALLLLIGLVCAPVVYAQNEEPQEPAAAPAPSEAKTKPKK
                     SKARNDFITLNFTNIDIGALIKVMSELTHRNFILDERVTGKVTLMTPTKISPEEAYQV
                     FLSALEIKGFTAMEDGKVIRIIPAATARQSGLKVLEDGDFEGQGFVTKLIRMNFVNPT
                     EIVRTITPLITKDGSIIAYPPTNSLIITDAVSNIRKIESLINAMDVAAPEGKGKINVY
                     YLKNASAEDIAKLMQALVSRLPVPPAGAAAAAPAGPSTILEGAVTITSDKATNSLIIV
                     GSPADYETMKDVIQKLDIRRRQVYVEAAIIEMSLAKQRELGFEFQVPVKSSSLVDNAT
                     TVGAVGGTNFGNIGGAIVNGPAGLVATNGLTVGAIKGTFTFQGKQYLSIGALLHALQT
                     DGNVNVLSTPNLLTMDNQKAEIMVGQNVPFITGQTQNAVTGSQTLFNTVNRQDVGIKL
                     SLTPQIASDDNVRLEVNQEISDVIASTLTNQAGPTTSKRSASTTVV"
BASE COUNT          575 a          706 c          678 g          416 t
ORIGIN      
        1 tccggcggag agcggccgtg ccgttcttca gccgcggcgc gagctttccg actattcgtc
       61 cattctggag cgcggtctct tcggcgaagg caaggccccg tcgagcggtc ccgcgacagc
      121 gacgtcggcc acatcctata aactcatcgg caccgttgag ggcgcggtct tctccggcgc
      181 cgtgctcgag gacggcaccg gccaggcctt ctatcgcaca aaccagaaac tgcccgacgg
      241 atcggtgatc gtaaaagtca tgcgcgataa aatttccttg aagaggccgg acggcgtgac
      301 ggagaacctt gaagtcgtag atgagacgaa gatcgtgccc atgaaggcgg ccggcacggc
      361 aagcgagggc ataaagaagc tctcgggcgg caagttcatg gtggaccaga aggaggtgct
      421 ggcttccacc gaaaacatga gccagatcct gacgcaggcg cgggccgtgc cctacacgga
      481 acagggaaag accgtcgggt tcaagctcag cgagatcgtg cccggcagca tctacgagaa
      541 gatcggactg gtgaacggcg acgtcatcca gcgggtcaat tctcaggaag tcgacgatcc
      601 ggccaaattc ttccagctct atcaggggct ccggaacgag aggaacatca cgatcgacct
      661 gatccggggc ggccagcgcc agaccctcaa ctacgagata cggtaaattc agccacagag
      721 gagaaataaa atcctaagca cgaagcccta aatcctaaat aatgaaccgc gcacggcaaa
      781 ggagtttggt atttaataat ccgtcattgt ttagtatttc gagcttcggg cttagaattt
      841 gaacgatctc tgcatctctg tggcctattc tttttttgga ggatacgagt gaaaaataaa
      901 gcggccctgc tgctcctgat agggcttgtg tgcgcccccg tcgtgtatgc ccagaatgag
      961 gaaccgcaag agcctgcggc tgcccccgcc ccatccgaag caaagaccaa acccaaaaag
     1021 agcaaagcaa gaaacgactt cattaccctt aactttacga atatagacat cggcgccctc
     1081 atcaaggtta tgagcgaact gacgcacagg aacttcatcc tcgatgagcg ggtgaccggc
     1141 aaggtcacgc tcatgacgcc gacgaagatc tcgcccgaag aagcctacca ggtctttctc
     1201 tctgccctcg agatcaaggg cttcaccgcc atggaggacg gcaaggtcat ccgcatcatc
     1261 ccggcggcca cggcccgcca gagcggcctc aaggtgctcg aggacggaga cttcgagggc
     1321 cagggcttcg tgaccaagct catcaggatg aacttcgtca atcccactga gatcgtccgt
     1381 accattacgc cgctcattac gaaggatggc agcatcattg cctacccccc cacgaactcg
     1441 ctcatcatca ccgacgcggt ctccaacatc cggaagatcg aaagcctgat aaacgcaatg
     1501 gacgtggccg cgcccgaggg aaaaggcaag atcaatgtct actacctgaa aaacgccagc
     1561 gccgaggaca tcgcgaagct gatgcaggcg ctggtgtcgc ggctgcccgt gccgcccgcg
     1621 ggagcagcgg ccgccgcacc cgccgggcct tcgacgatcc tggaaggggc cgtcaccatc
     1681 acgtcggaca aggcgaccaa ttcgctcatc atcgtcggat cgccggccga ttacgagacg
     1741 atgaaggacg tgatccagaa gctcgacatc aggcgccgcc aggtctacgt ggaggccgcc
     1801 atcatcgaaa tgagcctggc aaagcagcgc gagcttgggt tcgagttcca ggtccccgtg
     1861 aaatcgagca gtctcgtgga caacgccacc acagtgggcg cggtcggagg cacgaacttc
     1921 ggcaatatcg gaggagcgat cgtgaacggg cctgccggac tcgtcgcgac gaacggcctg
     1981 accgtcggcg cgatcaaggg cacctttacg ttccagggga agcaatactt gagcatcggc
     2041 gcgctcctgc atgcgctcca gaccgacggg aacgtgaacg tgctgtcgac gccgaacctt
     2101 ctcaccatgg ataatcagaa ggccgagatc atggtcggcc agaacgttcc ttttattacc
     2161 ggccagacgc agaatgcggt aacaggcagt cagaccctgt ttaacaccgt aaatcggcag
     2221 gacgttggaa tcaagctgtc tcttacaccg cagatcgcct ccgatgataa tgtccggctt
     2281 gaagtcaatc aggaaatttc cgacgtgatc gcgtccacgc tgacgaatca agcaggaccc
     2341 acgaccagca agcgctctgc gagcaccact gttgt
//
LOCUS       DASSLD010000147         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_736922,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000147 DASSLD010000000
VERSION     DASSLD010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736922"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /gene="prfC"
                     /locus_tag="VFK23_02785"
     CDS             <1..479
                     /gene="prfC"
                     /locus_tag="VFK23_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015027306.1"
                     /note="stimulates the release of release factors 1 and 2
                     from the ribosome after hydrolysis of the ester bond in
                     peptidyl-tRNA has occurred; GDP/GTP-binding protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET7318042.1"
                     /translation="IPTFSPEVFKELVNLDPSKSKQLAKGIEQLTDEGVAQLFTQQQG
                     NRRIVGTVGVLQFDVLQYRLEHEYGAKCRFAPMAIHKACWITADDQAAMDKFCRFKSD
                     RVATDKDGNTVFLAESDWVLRNMIEANPGITFHFTSEFKREMQEENPARERALSRS"
     gene            complement(560..1912)
                     /locus_tag="VFK23_02790"
     CDS             complement(560..1912)
                     /locus_tag="VFK23_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HET7318043.1"
                     /translation="MRQTKIVLSDKEIPTKWYNIMADMPNLPKPPLHPGTKQPVGPDD
                     LKPIFPMDLILQEVSQERWIAIPEEVLDIYSLWRPSPLFRAHRLEKALGTPAKIYYKY
                     EGVSPAGSHKPNTSIPQAYYNKKAGIRRIATETGAGQWGSSMALAGKLFGLDVTVYMV
                     KVSYGQKPYRRIMMETWGATVHASPSKVTNAGRKFLEMDPDNQGSLGIAISEAVEDAA
                     THNDTNYALGSVLNHVCMHQTVIGLEAKKQMELAGDYPDIVIGCCGGGSNLAGVSFPF
                     IHDKIGGKNLRVIAVEPASCPTLTKGEFRYDFGDVAGMTPLMTMYTLGHDFMPPGIHA
                     GGLRYHGDSPLVSQLYHDKLIEARAYTQIPVFEAAMQFANTEGIIPAPESAHAIRGAI
                     DEALLAKEEGKQKTILFNLSGHGYLDLQAYADYHDGKLQDYEYPAEKIKEALAKLPVL
                     "
BASE COUNT          439 a          646 c          690 g          488 t
ORIGIN      
        1 gcattccgac gttttcgccg gaggtcttca aggagctggt gaacctcgac ccgtccaagt
       61 ccaagcagct tgcgaaaggc atcgagcagc tcacggacga aggcgtggcc cagctcttta
      121 cccagcagca gggaaacagg aggatcgtcg ggaccgtggg ggtgctccag ttcgacgtac
      181 tgcagtaccg cctggaacac gagtacgggg caaaatgccg cttcgccccc atggcgatcc
      241 acaaagcatg ctggatcacc gcagacgatc aggcggccat ggataaattc tgcaggttca
      301 aatcggaccg cgtcgccaca gacaaggacg gaaataccgt gtttctcgcc gaatcggact
      361 gggtcttacg gaacatgata gaggccaacc ccgggatcac gtttcacttc acctccgagt
      421 tcaagcgaga gatgcaggaa gaaaaccccg ctagagaacg agccctctca cgatcttaga
      481 tcacgccgcc gcacctttca ttgcgcacca taaaaaaagc cccccgcagg aagcgggggg
      541 cccgtaacta tcactgggtc tacagcaccg gcagtttggc cagagcttct ttaattttct
      601 cggcgggata ctcgtagtcc tggagcttgc cgtcgtgata atcggcgtag gcctgcaggt
      661 cgaggtagcc gtgcccggag aggttgaaga ggatggtttt ctgcttgccc tcctccttgg
      721 ccaggagcgc ctcgtcgatg gctccgcgga tggcgtgggc gctttcgggc gctgggatga
      781 tgccttccgt gtttgcgaac tgcatcgcgg cctcgaacac cgggatctgg gtgtacgccc
      841 gcgcctcgat gagcttgtcg tggtagagct gggagacaag cggcgagtcg ccgtgatagc
      901 gaagcccccc cgcatggatg cccgggggca tgaagtcgtg cccgagagtg tacatggtca
      961 tgagcggcgt catccccgcc acatcgccga agtcataacg gaactcgccc ttggtgaggg
     1021 tcgggcagga cgcaggctcg acagcgatca cgcgcaggtt cttgccgccg attttgtcat
     1081 ggatgaacgg gaagctgacg cccgccaggt tgctccctcc cccgcagcag ccgatgacga
     1141 tatcagggta atcacccgcg agttccatct gtttcttcgc ctcgagcccg atcacggtct
     1201 ggtgcatgca cacatggttc aggacggagc ctaaggcgta gttcgtatca ttgtgcgtag
     1261 ccgcgtcctc cacggcctcg gagattgcga tgccgagact cccctggttg tcggggtcca
     1321 tctccaggaa cttcctgccc gcgttcgtca ccttggaggg gctggcgtgt acggtcgcgc
     1381 cccatgtctc catcatgatc ctccggtagg gcttttgacc gtagctcacc ttcaccatgt
     1441 aaaccgtgac gtcgaggccg aaaagctttc ccgccagcgc catggacgaa ccccactggc
     1501 ccgcgccggt ctcggtggcg atgcgcctaa tccccgcttt tttgttgtaa tacgcctgag
     1561 ggatggaggt gttcggctta tgggacccgg ccgggctcac cccctcgtac ttgtagtaga
     1621 ttttcgccgg cgtaccaagg gccttttcga gccggtgggc ccggaacagc ggagaaggcc
     1681 tccacaaaga atagatgtca aggacctctt cggggatggc gatccagcgt tcctggctca
     1741 cttcttgaag gatgaggtcc atggggaaga tgggtttgag gtcgtcgggg ccgacgggct
     1801 gtttcgtgcc cggatggagc ggcggcttgg gcaggttcgg catgtccgcc atgatgttat
     1861 accacttggt cggtatttcc ttgtcggaaa gaacgatttt cgtctgtctc attacttcct
     1921 cctgcgcggt aaagtcgaat gcgggtgacg caaaggtgag ccattctata ctatttcagg
     1981 cggtttctca acggtatttt agggtggact gatgctgaat tcatcgggta tcgccgcttt
     2041 gtcatccccg aaagacttta tccccgattc aagcattcga ggacggatct ggcgggcggc
     2101 tgccggaaga tcagaggcct atggaaaaca tgctctccag atcgtggcgg gaatagacgc
     2161 ggaaggcgat gagcgtttcc gacttctgga taccttccac cttcagcatg tggttggtaa
     2221 cgagttcggc gagctggtcg ttgtccttga cccggatgat cac
//
LOCUS       DASSLD010000148         2829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_149811,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000148 DASSLD010000000
VERSION     DASSLD010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149811"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFK23_02795"
     CDS             <1..203
                     /locus_tag="VFK23_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318044.1"
                     /translation="DIPPDLLFNISLIFIGKLLSTTSAYAAERGLYETEPVTTLATAS
                     AGTCRWGQVWELGMRRRKSITF"
     gene            complement(241..627)
                     /locus_tag="VFK23_02800"
     CDS             complement(241..627)
                     /locus_tag="VFK23_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HET7318045.1"
                     /translation="MSKAGNAKRKRFAVQLSTGVRDVGKVKSALMFATLAQYSGCDTV
                     VYCVQDGADALVKGAIREEGLPPGVPTFEQRLREAREAGVRFQLCEQTAINRNLTERD
                     LIEGAEITGGIHLIVYALEYDGVLYF"
     gene            1002..1790
                     /locus_tag="VFK23_02805"
     CDS             1002..1790
                     /locus_tag="VFK23_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7318046.1"
                     /translation="MNQENLIALKDWFAGYAASFSTPAAEEQRNIAIKRDHTYEVCGN
                     ALRIARDLGLSEGEALLAEAVALLHDVGRFSQYRQYRTFDDSVSVNHAALGVKVLLET
                     KALEGLPQHDRDLIVRAITLHNVFSLPEGLDETTLLFARLIRDADKLDVMRVVLEFFG
                     QKEESRADAVGLGLPDEPGYSPVVVASLARREMAKKSDLTTLNDFKLLQLAWLYDLNF
                     ASSLRMVLERGYIDRLAGLLPDTDEIRRAVAVVRAAVDERLCVG"
     gene            2052..>2829
                     /locus_tag="VFK23_02810"
     CDS             2052..>2829
                     /locus_tag="VFK23_02810"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010871000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7318047.1"
                     /translation="MRNNIVSLGADELKYEIREIVAVAKEFARLGIEINWENIGDPVQ
                     KGEKLPDWIKEIITGMMKEDLTYAYSPTKGLDATREYLAQKANKNGGVKITKEDIIFF
                     NGLGDAISKVYGQLRKEYRVIGPSPAYSTHSSAEASHAGSEYVSYRLDPDNHWYPDLT
                     DLRMKVKYNPSISGILVINPDNPTGAVYPREVLLEIVAIAREYDLFVVCDEIYINLTY
                     NGTKAVPLADVINGVCGISMKGISKELPWPGARCGWIEVYN"
BASE COUNT          639 a          838 c          802 g          550 t
ORIGIN      
        1 aggatatccc ccccgacctt ctttttaata tctcgctgat cttcatagga aagcttcttt
       61 caactacttc cgcctatgcc gccgaaagag ggttatacga gacggaacca gtgaccacgc
      121 ttgcaacggc gagtgccgga acctgtcgat ggggtcaagt atgggagctc ggaatgaggc
      181 ggcggaaaag cataacgttc tgacgtcagt catgccccgg cattattcgc ggcgcgggac
      241 ctagaaatag agcaccccgt cgtactcaag cgcatagacg atcaggtgga ttccgccggt
      301 gatctcggcg ccttcgatca ggtctctctc ggtaaggttt ctgtttattg ctgtctgttc
      361 gcacaactgg aacctgaccc ctgcctcgcg ggcctctctc aggcgctgct caaaagtcgg
      421 gacccccggc ggcagcccct cttccctgat cgcacccttg acgagggcat cggcgccatc
      481 ctgcacgcag tagacgacgg tatcgcaccc ggagtactgg gcgagggtgg cgaacataag
      541 cgccgatttc actttgccca cgtcccggac cccggtggac aactgcacgg cgaatctctt
      601 tcttttcgcg ttaccggctt tgctcatatc accctccctc tgcggcggca agtcatgtca
      661 agcttcaacc acagggaaca cagagaacat cctgaattga gtgagtaaaa ccatctttag
      721 acctttcatg agcgcacaga gaatgttttt ggggtgcagg ttttccccgg gcattcctca
      781 gtgtcctctg tggtttcata cttgcctctt ccgttttcat aaaagtcatg acagaaccct
      841 ggcggcaacc tcacgccggc ggagaaagac aaggaaaccc tcgctgtatg atacgacgga
      901 cggcagaata ttgccatcaa agaggaggct cttcgaccgc ggcatgcttg actcgtctgt
      961 cccattgcag tacaatcacc cgtccccgga aacacgcgcc catgaatcag gaaaacctta
     1021 tcgcactcaa ggactggttt gccggctacg cggcctcttt ttccacgccc gccgcggaag
     1081 aacagcgaaa catcgccata aagcgggacc acacgtacga ggtctgcggg aacgccctgc
     1141 ggatcgcccg tgacctgggg ctctccgaag gggaagcgct cctcgccgag gcggtcgccc
     1201 tgctccacga tgtcgggagg ttttcccagt accggcagta ccgcaccttc gacgacagcg
     1261 tctcggtgaa ccatgcggcc ctcggtgtaa aagtgctcct ggagacgaag gcgcttgaag
     1321 gccttccgca gcacgaccgg gacctcatcg ttcgcgccat tacccttcat aatgtctttt
     1381 ccctcccgga aggactcgac gagacgaccc tgcttttcgc caggctcatc cgcgacgcgg
     1441 acaagctcga cgtcatgcgg gtggtactcg agtttttcgg gcagaaggaa gagagcaggg
     1501 ccgatgccgt cgggctcggg ctgccggatg aaccggggta ttctcccgtc gtcgtggcca
     1561 gtctcgccag gagggagatg gcaaaaaagt ccgatctcac caccctgaac gacttcaagc
     1621 tgcttcaact cgcctggctg tatgacctca attttgcttc gtcgctcagg atggtgctgg
     1681 agcgcgggta catcgaccgg ctggcaggtt tgctgccgga caccgatgaa atcagacgtg
     1741 ccgtcgccgt ggtccgggca gctgtggacg agaggctgtg tgtgggctga cggtcccggg
     1801 cgggagaaag ccaaaattag gctttactcc gcaccaacta ttttggtaag ataaacagca
     1861 catcaaaggc tgatttcacc acagaggatg cagagaaggt agagaccggt cagaggtata
     1921 agcgggaagc gagaaaacac cgaatcggag aaacggggaa atagggagaa aaaaatcgat
     1981 gcgaatgcac ggttttttca cccattcgcc gattcgcccc tcggcggttc gtttctctaa
     2041 aaaggagcac tatgagaaac aacatcgttt cactcggggc cgacgagctc aagtacgaga
     2101 tccgggagat cgtcgccgtt gccaaggaat tcgcaaggct ggggatcgag atcaactggg
     2161 agaacatcgg cgacccggtg cagaaggggg aaaagctccc ggactggatc aaggaaatca
     2221 tcaccggcat gatgaaggag gacctgacct acgcctattc gcccacgaag ggcctcgacg
     2281 ccacgcggga gtatcttgcc cagaaggcga acaaaaacgg cggcgtcaag atcacgaagg
     2341 aggacatcat cttcttcaac ggcctgggcg acgccatcag caaggtctac ggacaactcc
     2401 ggaaagagta ccgcgtcatc ggccccagtc cggcttacag cacgcactca tcggcggagg
     2461 cctcgcacgc gggttcggag tatgtctcct accggctcga tccggacaat cactggtacc
     2521 cggacttgac cgacctcagg atgaaggtga aatacaatcc gtccatctcc ggcatcctcg
     2581 tcatcaaccc cgacaatccc acgggcgcgg tctacccgcg cgaggtgctg ctcgaaatcg
     2641 tcgccatcgc gagagagtac gacctcttcg tagtctgcga cgagatctac atcaacctca
     2701 cctacaacgg gacgaaggcg gtgccgctgg ccgacgtgat caacggcgtg tgcggcatct
     2761 ccatgaaggg catatcgaag gaactcccct ggcccggcgc ccggtgcggc tggatcgagg
     2821 tctacaacg
//
LOCUS       DASSLD010000149         3168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_703389,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000149 DASSLD010000000
VERSION     DASSLD010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703389"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFK23_02815"
     CDS             complement(<1..270)
                     /locus_tag="VFK23_02815"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HET7318048.1"
                     /translation="MHQDKIFGINPVAEALRSGRPMQRILIAEQRKAGREVHEIVRLA
                     KERGVEVRWAPRDALNREAPNASHQGIVAIVSATQYAILDDVLRIP"
     gene            complement(401..1876)
                     /gene="cysS"
                     /locus_tag="VFK23_02820"
     CDS             complement(401..1876)
                     /gene="cysS"
                     /locus_tag="VFK23_02820"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET7318049.1"
                     /translation="MIKVYNTLSGKKEDFIPIKSGKVSMYACGVTVYDFSHLGHARGA
                     VAFDLIQRYFRRRGFDVTYVRNFTDVDDKIINRAKEEGISPAEVAAKYIAAYQEDMKR
                     LGVGRADVEPKATEHIAEMIDVIKGLVEKGHAYAVGGDVYFRVSSFKEYGKLSKRNLE
                     ELKAGARVEVDERKQDPLDFALWKSSKPGEPAWDSPWGPGRPGWHIECSAMGLRHLGE
                     TFDIHAGGKDLIFPHHENEIAQSEAYSGRKFVNYWLHNGFVNINQEKMSKSLGNFFTI
                     RDILNQYDAEVVRLFLLSTHYRSPIDFSDANLKDARAGLDRFYTMKEGIIKFLAGKET
                     PSVKPEEVIEVADRPLYEKIQNLARAFEEAMDDDFNTAFAIGLIYDLVRDINKYLADA
                     DKKNQDAVYCVLSAASASFENVSRTLGIFLRDPAEWFKEGRLADAKVTIPTEQIEEYI
                     HLRNEARARKDWAEADRIRKILDDGGVELFDRADGTVWKPK"
     gene            complement(1995..>3168)
                     /locus_tag="VFK23_02825"
     CDS             complement(1995..>3168)
                     /locus_tag="VFK23_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HET7318050.1"
                     /translation="SGLPPFTGGAVGFFSYDFVHQLESLPHTAVPDLDIPEAYFLFVD
                     LVVAFDHVQNKSWVIVNPGAREQELGFRRPGPREWEGLYDRAADRLTAVRSKLVAINS
                     ADDAPLSEKSIRRHFSLTPYMTQDRFESMVKRCKEYIAAGDIYQANLSQRLSAAIGDA
                     DPLRLYRMLRTVNPSPFAAFLDFDDLQLVSSSPERLVRLRDGIADTRPIAGTRRRGGD
                     EAETHELSAELLTNEKERAEHIMLLDLERNDLGKVCDYGTVAVDEMMVVEDYSHVIHI
                     VSNVRGSLAPGRDAFDLVRAVFPGGTITGVPKVRCMEIIDELEPVARGPYTGSIGYIS
                     NTGDMDLNIIIRTFVIKNGNAHVQVGAGIVADSDPAREYFETMQKAEALKKALELL"
BASE COUNT          642 a          898 c          882 g          746 t
ORIGIN      
        1 cggtatcctg aggacatcat ccagtatcgc atactgcgtt gccgatacga ttgcgacgat
       61 accttgatgc gaggcattcg gcgcctcccg gttcagagca tcgcggggcg cccatcttac
      121 ctcgacgccc cgttcttttg ccagacggac aatttcatgg acttcgcggc ctgccttccg
      181 ctgctcggca atcaggatcc gctgcatagg ccgaccggac ctcagggcct cggctaccgg
      241 attaataccg aatattttat cttgatgcat aggatgaagt gccaagtcgc agagatcata
      301 tagtatgaag gttcaaaaag ccagtgacgg aaacgctgaa gttttcgaat ttgtgttcgg
      361 gatttattta tgatatggat tgctttgtgc ttgtgaatgg ctatttcggt ttccagacag
      421 ttccatcggc ccggtcgaaa agttccacgc cgccgtcatc gagaatcttc cggatcctgt
      481 ccgcctcagc ccagtccttg cgggcccgtg cctcgttgcg aaggtgaatg tattcttcga
      541 tttgttcggt cgggatcgtg accttggcgt cggcgagacg gccttcctta aaccattccg
      601 cgggatcgcg aagaaaaatg ccgagggtcc tgctcacgtt ttcgaaggag gctgatgccg
      661 cggacaggac gcagtatacc gcatcctggt ttttcttatc cgcatctgca agatacttgt
      721 tgatatcgcg cacaagatcg tagatgaggc cgatggcgaa agcggtatta aaatcatcat
      781 ccatggcctc ttcaaacgcg cgggcaaggt tctgtatctt ctcgtacagc ggccggtctg
      841 cgacctcaat gacctcttcc ggcttgaccg agggcgtttc ctttcctgcg agaaacttga
      901 tgatgccctc tttcatggtg tagaaacgat cgagtccggc gcgagcatcc ttcaaattcg
      961 cgtccgagaa gtctatcggg ctgcggtagt gcgtcgacag gaggaacagc cggactacct
     1021 cggcatcata ctggttcagg atgtcgcgga tcgtgaaaaa gttgccgagc gacttggaca
     1081 tcttctcctg gttgatgttg acgaaaccgt tgtgaagcca gtagttcacg aacttcctgc
     1141 ctgaataggc ctcgctctgc gcgatctcgt tctcatggtg cggaaagatg aggtccttgc
     1201 cgcccgcgtg gatgtcgaag gtttcgccca gatgcctaag ccccatggcg gaacattcga
     1261 tgtgccagcc gggtcgcccc gggccccagg ggctgtccca ggcgggctct cccggcttcg
     1321 acgacttcca gagagcgaag tcgaggggat cctgttttcg ttcgtctact tcgacgcggg
     1381 cgcctgcctt cagttcttcg aggttccgtt tggagagctt gccgtactcc ttgaaactgg
     1441 atacgcggaa atacacgtcg cctccgacgg cataagcgtg ccccttctct acgaggccct
     1501 tgatgacgtc gatcatctcc gcgatatgct ccgtggcctt gggctccaca tccgccctgc
     1561 cgacgcccag gcgcttcatg tcctcctgat aggcggcgat atatttggcg gcaacctcgg
     1621 cgggactgat gccctcttcc ttcgcgcggt tgatgatctt gtcgtcgaca tcggtgaaat
     1681 tccgcacgta cgtgacgtcg aaccctcttc gccgaaagta gcgctggatc aggtcaaagg
     1741 ccacggcccc gcgcgcatgg ccgagatggg aaaagtcgta gaccgtcacg ccgcaggcgt
     1801 acatggagac cttgcccgac ttgatgggga tgaagtcctc tttctttccc gagagcgtgt
     1861 tataaacctt gatcatgaat aaaaaaatcc ctttttctgc gattgccgtt tttatgatga
     1921 acaggaaata tgattttatc cggtgtccat ctggtttgtc aagccttttc aatttcttaa
     1981 aaaaatgcct tacgctacaa caactcaagc gccttcttga gggcttcggc cttctgcatt
     2041 gtctcgaaat attcccgcgc gggatcggag tcggcaacga tccctgcgcc tacctgtaca
     2101 tgtgcgtttc cattcttgat cacaaacgtc cgaatgatga tattgaggtc catgtcaccc
     2161 gtgttcgaga tatagccgat cgatccggtg tacggcccac gcgccaccgg ttcgagttcg
     2221 tcgattattt ccatgcagcg caccttcgga acgcccgtta ttgtcccgcc cggaaatacg
     2281 gcgcgcacga gatcgaaggc gtctctcccc ggcgcaagtg aaccccggac attcgagacg
     2341 atatggatca cgtgggagta gtcctccacg accatcattt catcgacagc gacggtgccg
     2401 taatcgcata ctttgccgag atcgttccgt tcgaggtcta ggagcatgat atgctcagcg
     2461 cgctctttct cattcgtcag gagttcagcg gagagctcgt gcgtctccgc ctcgtcgcct
     2521 cccctccggc gcgtgcccgc aatcggcctc gtgtcggcga tcccgtcgcg gagccgcacc
     2581 aggcgctcgg gcgaggaact cacgagctgc agatcgtcga aatcaaggaa ggccgcgaag
     2641 ggagaaggat tgacggtccg cagcatgcgg taaaggcgaa gcgggtccgc gtcgccgatg
     2701 gctgcggata gccgctggga taggttggcc tgatagatgt ctcccgcggc gatatactcc
     2761 ttgcaccgtt tcaccatgga ttcgaagcgg tcttgtgtca tgtacggcgt aaggctgaaa
     2821 tgacggcgga tgcttttctc ggacaacggg gcatcgtccg cactgtttat tgctacgagc
     2881 ttcgagcgaa ccgctgtgag cctgtccgca gcccggtcat aaagcccttc ccactctcgc
     2941 ggcccgggtc gccggaaccc cagttcctgc tcccgggcgc cgggatttac gattacccag
     3001 ctcttgtttt ggacatggtc gaatgcgaca acaaggtcca caaataaaaa ataggcttcg
     3061 ggaatatcaa ggtcgggaac ggccgtatgc ggaagagatt caagctgatg gacgaagtcg
     3121 taggaaaaaa atcccaccgc cccgcccgtg aaggggggaa ggcccgaa
//
LOCUS       DASSLD010000150         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_344900,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000150 DASSLD010000000
VERSION     DASSLD010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344900"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VFK23_02830"
     CDS             <1..959
                     /locus_tag="VFK23_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7318051.1"
                     /translation="TKLLGLARKSKYEMTPINVNDVVYDTVKLLEQVVDKRISVSVEL
                     ANPLPTIMGDANQIEQIIMNFIVNARDAMPKGGRIVIHTSARTVVKGMLDVPPYVESG
                     DYVLLSVTDTGMGIPEEIINKIFEPFFTTKEQGKGTGLGLSMVYGAVKDHQGYLAVQS
                     TVGVGSVFTVYLPAAKPAASPLMKAPAKGKETLLVIDDEESLLEVMREALEGNGYKVF
                     ATSESNVALDIYRRIFHEISLVVTDIVMPHMDGKELIRQIKEINPEVKVLAVSGYTKY
                     VAGKEEIRDIEGFLHKPFESYYLLSVVRRILDAKPKNFLPPM"
     gene            complement(1096..1533)
                     /locus_tag="VFK23_02835"
     CDS             complement(1096..1533)
                     /locus_tag="VFK23_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET7318052.1"
                     /translation="MDELKNAQEGIAPAQIIQELRATFREEAYKLLKELEAALVEIKK
                     RPDDKKQIEKAFRAMHTITGSGGACEFHDIVDFTRQLETIFVRIKSGKARANREIVDL
                     ALSARDQIKAMLDSYYYGGSVDDSRADGILASLKNLAQGGRKA"
     gene            complement(1740..>2340)
                     /locus_tag="VFK23_02840"
     CDS             complement(1740..>2340)
                     /locus_tag="VFK23_02840"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET7318053.1"
                     /translation="ALIITEMVLPGMGGVDLFHLLRQSRRTAFIPVVFMIHPTDADSE
                     RRCLGTGAAGCITKPVQADELYRTVQSVIEPIRRQDIRIDTRLFVSVNDVPLDCEAGG
                     CEIELSEGGMYVPMDNPYARDKRITVRFNIRDRTICVEGSVVYSHASGVVPHKESGMG
                     LKFVNIEAADREFIRQFIRDEVMKDIRSSSAPETSDPGE"
BASE COUNT          520 a          675 c          658 g          487 t
ORIGIN      
        1 tcacgaaact gctgggcttg gcccgcaaga gcaaatacga aatgacaccg atcaacgtga
       61 acgatgtcgt gtatgacacg gtcaaactgc tcgagcaggt cgtggacaag agaatcagcg
      121 taagtgtcga gctggccaat ccgctgccga ccatcatggg cgacgccaat caaattgaac
      181 agatcatcat gaatttcatc gtcaacgcgc gcgatgcaat gccgaagggc gggcgcattg
      241 taatccacac ctcggcgagg accgtcgtca agggtatgct ggacgtgccg ccctatgtgg
      301 agtctgggga ctatgtgctg ctcagcgtca ccgacaccgg catgggcatc cccgaggaga
      361 tcatcaacaa gatcttcgaa ccttttttca cgacgaaaga gcagggaaag ggcaccggac
      421 tcggcctctc catggtctac ggcgccgtca aggatcatca ggggtatctc gcggtgcaga
      481 gcacggtcgg cgtaggaagt gttttcacgg tctacctgcc cgccgcaaag cccgcagcct
      541 cgccgctcat gaaggctccg gccaagggga aggaaacgct gctggtcatc gacgacgagg
      601 aaagcctgct ggaagtcatg cgggaggcgc tggaaggcaa cggatacaag gtgttcgcaa
      661 ccagcgagtc caacgtcgcc ctggacatct accggaggat ctttcatgag atctccctcg
      721 tagtcacgga tattgtgatg ccccacatgg acggcaagga gctcatacgc cagataaaag
      781 agatcaatcc tgaggtgaag gtgctcgcgg tttccgggta tacgaaatat gtcgcgggca
      841 aggaggaaat cagggacatc gagggttttc tccataaacc ctttgaatcc tactatcttc
      901 tttccgtagt gaggcgcatc ctcgacgcga agccgaaaaa cttcctcccc cccatgtgat
      961 gcgtcctttc acgacccatc gcacagagcg ttggtagtac gaacaaagta aaatcaagag
     1021 gaattgaagc cgcggaatgg cggaaaaaga ccacccggtc gatggcgggg aaacgcttct
     1081 atcagcggag accgttcaag ctttccgtcc gccctgcgcc aggtttttca gggaggcgag
     1141 aatgccgtca gcccgtgagt cgtcgacgga accgccatag tagtagctgt cgagcattgc
     1201 tttgatctga tcccgcgcag acaatgccag atcgacgatc tcccggttcg cccgcgcctt
     1261 cccgctttta atcctgacga agatcgtctc cagttggcgc gtgaagtcga caatgtcatg
     1321 aaattcgcac gctccgccgg agcccgttat ggtgtgcatc gcacgaaagg ccttttcgat
     1381 ctgtttctta tcgtcaggcc ttttcttgat ctcaaccaat gccgcttcga gctccttcag
     1441 gagcttgtag gcttcttccc ggaacgtcgc acgcagttcc tgaataatct gggcaggggc
     1501 aataccctcc tgggcatttt tcagttcatc cacggttcgc tcccgccgcg gcaatcaaca
     1561 cacggctctt ttccgcacca agattgcatg aagccggccg cgtcttgatc gatccgggcc
     1621 acacgtttgc cgcgtggacg gcctgtcacc gcttcagcgt ctcgatccgc agctttttat
     1681 tttttcgtca cgtgaaagat tgaagggaag ccgaagcaag attgcccggg ccaaaccggt
     1741 cactccccag ggtcggatgt ttcaggggcc gatgacgatc gaatgtcttt catgacttcg
     1801 tcgcggatga actgcctgat gaattcccgg tccgccgcct cgatattgac gaatttgagc
     1861 cccatgccgc tctccttgtg cggtacaacg ccggaagcgt ggctgtagac aacagagcct
     1921 tcaacgcaga tcgtgcggtc cctgatattg aaccgcacgg tgatccgttt gtccctcgca
     1981 taggggttgt ccatcggcac atacatgccg ccttcggaca attcgatctc gcatccgccg
     2041 gcttcgcagt cgagcggcac gtcgttgacc gacacgaaca accgagtgtc gatccggatg
     2101 tcctgacgcc ggatcggctc aatgacggac tgaacggtcc ggtagagctc atcggcctgc
     2161 accggcttgg taatgcaacc ggcagcgcct gtcccgagac atcgtctctc ggagtccgcg
     2221 tctgtcggat ggatcatgaa gacgacgggg atgaaagcgg tgcgtctgga ctgccggagc
     2281 aggtgaaaca ggtcaacccc acccataccc ggcaggacca tctcggtgat aatgagggcc
//
LOCUS       DASSLD010000151         3769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_312622,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000151 DASSLD010000000
VERSION     DASSLD010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312622"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2283)
                     /locus_tag="VFK23_02845"
     CDS             complement(<1..2283)
                     /locus_tag="VFK23_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7318054.1"
                     /translation="MGWFSEIFSDVRERIRGLGPRNTIAIPIVLLLLFIGAGILTAGY
                     YINKSVVHAVFEERESNKARNIHLTIESIVSTEVNRISSLAKILKNDTDIVYGLFHYS
                     GTQGDTGPLKSAMSQIYPKMNLRVFVMTDTNGKVLYRAGKQKVPGEENLEKMPAFKRA
                     LKGDQVITAIHTPEGFGICVIVPVYAFKKAKPSGLMILGNRIDDDFAKKIARETGTQV
                     FLATPDGVFAGSQANGFAEAFNPPMATKSLEEQRPIFHMDRKNYRSYTYVPMRIVDEQ
                     FCLVIETDTSVIKDLLAMNRTKMTQLGFILLIGITMVGFGLTLLLVWPLNRLRGKALE
                     TIREYSGAGVDTISRGNEFSTLVRANDIMLETIKNHLTERTRAEDALRETSHTLHALI
                     DASPLAVVVSDASGTVRVWNPAAVRMFGWTEAETIAHPNPLLSPGGNPELRETYSLVL
                     HGERISNTEICARTRAGSEIFLAFSGAPLFDTQGEIISMMAIMADITDAKTAEESLRR
                     SEEQLRQSLKMEAIGKLAGGVAHDFNNLLSVITGYSELLLTRTGGRNPARRELKEIHK
                     AAERAAALTQQLLAFSRRQILKPKRLRMNEVVENMGKMLLRLIGENIDFSTESQEDLW
                     TVRADPSQIEQILMNLCVNARDAMPAGGKLVVSTANVTLEEPLVDRELTIPSGRYATL
                     RVADNGSGMDEKILSKIFEPFFTTKDQGKGTGLGLATVYGIVKQSEGYIRVVCAPGEG
                     STFSVYLPAAEEEEHEAEEDR"
     gene            complement(2924..3169)
                     /locus_tag="VFK23_02850"
     CDS             complement(2924..3169)
                     /locus_tag="VFK23_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318055.1"
                     /translation="MQTVTVYRVDYARKTKYPIGVVLERRETERRNNYQDMLRLARRL
                     FALDPADFANIVIDISQVRRTFPEQHRGARPDLRETA"
BASE COUNT          713 a         1106 c         1072 g          878 t
ORIGIN      
        1 ccggtcctct tccgcttcgt gttcttcctc ctcggcagcc ggaaggtaga cggagaaggt
       61 cgatccttct ccgggggcgc agacgacgcg gatgtacccc tcgctctgtt tcacgatccc
      121 atacaccgtg gccaacccca ggcccgtccc tttcccctga tctttcgtgg taaagaacgg
      181 ctcgaagatc tttgagagga tcttctcgtc catgccgctc ccgttgtcgg caacccggag
      241 agtcgcgtag cggcccgaag ggatggtcag ctcccggtcc acgagaggct cctccagcgt
      301 gacgttcgca gtggaaacga ccagcttccc tcccgctggc atggcgtcgc gtgcgttgac
      361 gcaaagattc atcaggatct gctcaatctg gctcgggtcc gcccggaccg tccacaggtc
      421 ttcctgcgat tccgtggaga aatcgatgtt ttctccgatc agccgcagga gcatctttcc
      481 catgttctcc accacttcgt tcatgcgaag cctcttcggc ttcaggatct gccgccggct
      541 gaaggcgagg agctgctgcg tgagggccgc cgcccgttcg gccgccttgt ggatctcctt
      601 gagctcccgc cgggcgggat tccggccccc ggtgcgggtc aggaggagct cgctgtaccc
      661 cgtgatcacc gagaggagat tgttgaagtc gtgggcgaca ccccctgcca gcttcccgat
      721 ggcctccatc ttcaaggatt gacggagctg ctcctcgctt cggcgcaacg attcttccgc
      781 cgtcttggcg tcggtgatgt cggccatgat cgccatcatg gagatgatct ctccctgcgt
      841 atcgaacagg ggggcgcccg agaaggcgag gaagatctcc gaaccggcac gggtccgggc
      901 gcaaatctcc gtgttggaaa ttctttcccc atgcaggacg agactgtatg tctcccgcag
      961 ctccgggttc cctccggggg aaaggagggg gttcgggtgg gcgatcgttt ccgcctccgt
     1021 ccacccgaac atccggacgg ctgccgggtt ccatacgcgg actgttccgg aggcgtcgct
     1081 tacgaccacc gccagagggg acgcgtcaat cagggcatgg agggtatggc tggtttcccg
     1141 caatgcgtct tcggcgcgcg tccgctccgt aaggtggttc ttgatggtct ccagcatgat
     1201 gtcgttcgcc cgcaccagtg tcgagaactc gtttccccgg gaaatggtat cgacccccgc
     1261 tccggaatac tcccggatgg tctccaacgc tttcccgcgc aagcggttca acggccagac
     1321 gagcaggagg gtgagcccga atccgaccat cgtgatgccg atcaggagga tgaaacccaa
     1381 ttgggtcatc ttggtgcggt tcatcgccag gagatccttg atgacactgg tatccgtctc
     1441 gatcacgagg caaaactgtt cgtcaacgat ccgcatcggc acgtaggtat aggaacggta
     1501 gtttttcctg tccatatgga agatgggtcg ctgttcctcc aggcttttcg tggccatcgg
     1561 gggattgaat gcctcggcga aaccgttcgc ctgggatcct gcaaacacgc cgtcgggagt
     1621 cgccaggaat acctgggtgc ccgtctcccg ggcaatcttc ttcgcgaagt cgtcgtcgat
     1681 gcggttgccg agaatcatca gccccgaggg cttcgctttc ttgaaagcgt agacgggtac
     1741 gatcacacag atcccgaatc cctcaggggt gtggatcgcc gttatgacct gatccccttt
     1801 gagtgccctt ttgaatgccg gcattttttc cagattttcc tctcccggca ccttctgctt
     1861 ccccgcgcgg tacaggactt tcccgttggt atcagtcatg acgaagactc gcagattcat
     1921 cttcgggtag atctggctca tcgccgactt cagtggcccg gtgtcgccct gtgttccgct
     1981 gtaatggaac agcccataca cgatgtctgt atcgtttttc aggatcttcg ccaggctcga
     2041 gatcctgttc acttccgtgg aaacgatgga ttcgatcgtc aggtggatgt tcctggcctt
     2101 gttgctctcc cgctcctcga acaccgcatg gacaacggat ttgttgatgt agtagccggc
     2161 ggtgagaata cctgcgccga tgaagaggag gagcaacacg atggggatgg cgatggtgtt
     2221 ccgggggccc agcccccgga ttctctctcg gacatcgcta aagatttctg aaaaccagcc
     2281 caaacagata cctcgcgggc atagggatgc cttttcccct cttatcgggg gtaagtctct
     2341 aaaacttcaa tcattacacc ctcccagcaa gtcggcaaaa aaaggaaatt cctatccttt
     2401 tcggggagcc atggcaagcc tcattggggt tttcgctaaa ttcgcgcgtg acttgatccg
     2461 agaacgggtg cgggcggggt tggagaatcg tgtaccactt ttcaatcttc ctcaggatcg
     2521 tcactcggtc tggatggcgc gtcagactgc gattggtccc gatggaattc ctgccatgct
     2581 ttcttctccg ctgcgaacac ctcaggatcg actaattccc tgttactgaa gggcgaaatg
     2641 ccggttgacc caatccctct tctcgtccag taccagggcc tttctaacaa gcatctgcgg
     2701 tatatggtga ctaatctcaa ccgcacgagt gttattgcat cggcaattcc atatgaccgc
     2761 cttgtccaga tgaatcccga gtggattgac agtttccagg tcgtctacct gtccgcactt
     2821 cgcgcaggag atggatgggg tcatttcgaa acgcgagaac acgtcagccg gaagtgtatt
     2881 tggctagcct gcatgggccg caaacccccc agttgcgtta gagtcacgct gtttccctca
     2941 ggtccggtcg ggctcctctg tgctgctccg ggaatgtccg ccggacttga ctaatatcga
     3001 taacaatgtt ggcgaagtct gccggatcca acgcgaagag tctacgcgcc agccgcaaca
     3061 tgtcttggta gttgttgcgc cgttccgttt ctcgcctctc caggacaaca ccaatgggat
     3121 atttggtctt cctggcataa tccacgcgat agacagttac cgtttgcatc gttgtctcct
     3181 ggattctctg atttcttgca agataatcct tcccgtcagc ctccggtatc ggcatctgtc
     3241 tgaaatgcga gtcgcagttt gtctgattgt agggcaggtg tttgtcctat gacttagagc
     3301 atcagaagga tgtggatgat gaccggagat cgtccagcat cggactcatg cgagggatga
     3361 gggagcattc cgcaagcggg tcacatctta taagcagggg aagatgctcg gagtagctgc
     3421 ttcaacgggg cagccgggcc gaatggcccc tgagcccctg actgcgtaga tgccgcttgc
     3481 cccggcgggc aagttagcga ggtgccttac cctcatcccg ttaatcctgc cggggcacct
     3541 ctttccctcc cggggagaac ctccccccct ctaaataccc actgaaggcc cacttcattc
     3601 aacgatgtga gccgtagagg tgctggctac gctggggagg gggatgcctg tagttgcgcc
     3661 acgcggtagg aatcaatcgg gcgaaatctc actcaggtga atgctcgcca gggcggaggc
     3721 gtctaaaaaa tgaataagaa cagattcgct caggagcggt tcaccgctc
//
LOCUS       DASSLD010000152         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_447376,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000152 DASSLD010000000
VERSION     DASSLD010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447376"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="VFK23_02855"
     CDS             <1..938
                     /locus_tag="VFK23_02855"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318056.1"
                     /translation="VGFIFIEIDPALVDVNVHPAKAEIRFRNQSQIHDLIRDVIREGL
                     RKHGVPVIVSPPQADAGKGVQEALAEYLKRQTDAVLEMKHSPWYGRRKNDPRATEPQP
                     SFSAETDSLPAQTALLPVHDLFPLAQIHESFIIAQSKDGMALIDQHAAHERILFEKLQ
                     DELDGGGTSIQHLLVPVQIELGPAQSGLLTEYLPELAKLGFAVEDYGNGTFIIKSVPA
                     LFSGADYKKLLLDILDEVNVHGKSGKMEELRNSILSVMACHPAIKVHRKLTVTEMERL
                     LHDLYQCRMPHTCPHGRPTVIRFSIAEINRMFKRL"
     gene            1223..2173
                     /gene="miaA"
                     /locus_tag="VFK23_02860"
     CDS             1223..2173
                     /gene="miaA"
                     /locus_tag="VFK23_02860"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET7318057.1"
                     /translation="MMSNDKPILIIAGPTAVGKTDASLLLARETGAEIVSADSMQIYR
                     GMDIGTAKPTLEQRRLVYHHMIDIAAPDQPYSVGDYLRDARAAIDGITASGGVPIVVG
                     GTGLYIRALTRGLFHGPPADLDLRERLLRRESEGQPGTLYSDLVKADPEAAVKIHPND
                     LRRTIRALEVYYLRDRRLSEFHREHAFKERPFRFRMIFLARSRSELYPRIEQRVDRML
                     ADGLETEVRTLMDRGYGPDLSSMQGLGYKQFINHFLGRTTRDEAVALLKRDTKRYAKR
                     QFTWFRREPSAVWVDITGLDKAEDIAERIKKSIEISNKLV"
BASE COUNT          518 a          647 c          645 g          428 t
ORIGIN      
        1 ccgtcggatt cattttcatc gagatcgacc cggcccttgt ggatgtgaat gtccatccag
       61 ccaaggcaga gatccggttc cgaaaccagt cgcagatcca tgacctgata agggacgtga
      121 tccgggaagg tctccgcaaa cacggggtgc cggtcattgt cagccctccg caagcggatg
      181 ccgggaaggg agttcaggaa gcactggccg aatatctcaa gaggcagacg gatgcagttc
      241 tggaaatgaa gcactcaccg tggtacgggc ggcggaagaa cgacccgaga gcaacggagc
      301 ctcaaccgtc cttcagcgcg gagaccgata gcctgcctgc gcaaaccgcc ctgcttcccg
      361 tgcacgatct gttccccctc gcccagatcc acgaatcatt catcattgcc cagtccaaag
      421 acgggatggc gcttatcgac cagcatgcgg cccatgaacg cattctgttc gaaaaactgc
      481 aggatgagct cgatggggga gggacatcga tccagcacct cctggtgccc gttcaaatcg
      541 agctcggacc ggcccaaagc ggactgctca cggaatatct gccggagctc gccaagctcg
      601 ggttcgccgt cgaagactac ggaaacggca cgttcattat caagtctgtc ccggcgctct
      661 tttccggagc agattataaa aaattgctgc tcgacattct cgatgaagtg aatgttcacg
      721 gcaagagcgg aaaaatggag gagctgcgca actccatcct gagcgtcatg gcctgccatc
      781 cggcgatcaa ggtgcaccgg aagctcacgg tgacagaaat ggaaaggctt cttcatgatc
      841 tgtatcagtg caggatgcct catacttgtc cgcacggcag gccgaccgtg atacggtttt
      901 cgatagctga gatcaacagg atgttcaaga ggctgtagct ccctcggcag tcgaacggcg
      961 cttccggcgt gaacaggttc gctccaagac cgacgagtcg gagatcaccc tgaccaatgt
     1021 gtccattgag ggagatctgg cgatccagaa agcgcacagc cgtcccgacc tggccgggcc
     1081 atgctcggcg ggaaaacagc cgctctcccg gcgaaaaagg gttcaacgtg ctcttttccg
     1141 tcagccgcat ttcatgcctc ctacagaggg ctatgttcac ggatcccgta aaattacacc
     1201 cggtaaccct ctctgaaccc gtatgatgag taatgacaaa cccatcctga tcatcgccgg
     1261 tcccacagcg gtcggcaaga ccgacgcatc acttcttctc gcacgggaga ccggtgcgga
     1321 gatcgtgagc gccgattcca tgcagatcta ccggggcatg gacatcggaa cggcaaagcc
     1381 cacgcttgag cagcgaaggc tcgtctacca ccacatgatc gatattgcag ctccggacca
     1441 gccctacagc gtgggcgact atctcaggga tgccagggcg gccattgacg gcattacggc
     1501 ttcgggtggc gtccctatcg tcgtcggcgg gacaggcctc tatatccggg ccctcacgcg
     1561 cgggctgttc cacggtcctc cggctgacct cgacctgcgt gaacgtctgc tgcggcgtga
     1621 atcggaaggg cagccaggca ctctctattc agatcttgtg aaggccgacc ccgaagccgc
     1681 ggtcaagatc catcccaacg atctccggcg cacgatcaga gcgctggagg tgtattacct
     1741 gagagacagg aggttgtccg aatttcatcg tgaacacgca ttcaaggaaa ggcctttccg
     1801 gttccggatg atcttcctcg cgcggagcag gagcgaactg tatccccgca tcgaacagcg
     1861 cgtggaccgg atgctcgccg atgggctcga aaccgaggtg aggaccctca tggaccgcgg
     1921 gtatggtcct gatctctcgt cgatgcaggg ccttggatac aagcaattca tcaatcattt
     1981 cctcggcagg acgacgcggg atgaggcagt ggccctgctc aagcgcgaca ccaagcggta
     2041 tgcgaagcgc cagttcacct ggttcaggcg cgaaccgtct gcggtgtggg tggacatcac
     2101 ggggcttgac aaggcagagg atattgctga gcgcataaag aaaagcattg aaatttcaaa
     2161 taagttagta taagattaca aaaaatattg tgtagcggca agaggccgca agaggcccgc
     2221 accgcagagt tcctgatt
//
LOCUS       DASSLD010000153         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_736283,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000153 DASSLD010000000
VERSION     DASSLD010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736283"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..1065
                     /gene="fabG"
                     /locus_tag="VFK23_02865"
     CDS             292..1065
                     /gene="fabG"
                     /locus_tag="VFK23_02865"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010492623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HET7318058.1"
                     /translation="MLNSIAGRSVVVTGSSKGIGKGIARVFAEKGCKVLIVARHYDEA
                     QRAAIEIRERGGTAIAFAADISDQRAAREMASAACKYHGGIDILCANAGIYPMARLEE
                     MTLSDWEGVIRTNLTGTYLSVDACLPELKRTGRGRIIIISSISGPVTAIAGLSHYGAS
                     KAGQLGFMRAAALELAAAGVTVNAILPGNIATEGVDVLGPDYISQMTAAIPLGRLGSV
                     EDIAYAALFLASDEAAYITGQTITVDGGQLLPESKTAMG"
     gene            1068..2045
                     /locus_tag="VFK23_02870"
     CDS             1068..2045
                     /locus_tag="VFK23_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET7318059.1"
                     /translation="MLENLNRLAQASLSRYELSPRATATLINYTENSTYRVDDPDTGN
                     RFIFRIYRLGYHDRQTIESELRWMSALRRNAGVQTAETVSARDGATLLLLSDDVLPQP
                     RFGVMFTFLNGTEPPEDHLPESFDRLGEVAARMHLHSRTWDLPSSFKRPTWNFESMLG
                     GTRPIWGRWQDGIGLDPEREKLLGRLTEVVARRLERFGIGREQFGLVHADLRLANLLL
                     DGNKTQVIDFDDCGFGWYLYDLSTALTFMEERTDAQKLVGSWVRSYRRLAPLTKDEEG
                     EIPTFMMLRRMMVVAWIGSHSDTELARSEGAGYTFGTCRLASRYLDRFA"
BASE COUNT          448 a          626 c          692 g          436 t
ORIGIN      
        1 atggccatgg aggagagggg agctccctgc ttgtaggaat attcacttat cccaggacag
       61 gacctacggg ttactgatct gcctgagaca cccatcttct cagccagttc cacctgcgat
      121 ctaccgtctt tccttgctgg aatcgttggt agctcaatgg tacccgcccg ccgtatcttg
      181 ggaaaattct tatgcccggc aatacgccaa tggaccgcat ggtaccatct tagaaattat
      241 atggtgatac tccggtgttg caggctggaa gagacattaa aggggggggg catgctcaat
      301 tcgattgcgg ggcgctctgt tgtcgtcaca ggttcaagca agggaatcgg caaaggaatt
      361 gcgcgtgtct tcgctgagaa ggggtgcaaa gtactcatcg ttgcgcgaca ttacgacgaa
      421 gcacagaggg cggctattga aatcagggag cggggcggca cagcgatcgc ttttgcggca
      481 gatatatcag atcaacgcgc cgcacgcgaa atggcatccg cggcatgcaa ataccacggc
      541 ggcatcgaca tcctgtgcgc caacgccggc atctacccga tggccagact cgaagagatg
      601 accctttcgg actgggaggg agtcatccgg acgaatctta ccgggacata cctgtcggta
      661 gacgcgtgcc tccccgaatt gaagcgcacc ggaagaggcc gtatcatcat catctcctcg
      721 atatccggtc cggtcaccgc gatcgccgga ctcagccact acggcgccag caaggcgggg
      781 caactcggct tcatgcgggc cgccgcgctg gaacttgcgg cagcgggcgt caccgtcaac
      841 gcgattcttc caggaaacat cgcgaccgag ggcgtggacg tacttggccc ggactacatc
      901 tcccagatga cggcggccat acctctcggg cgccttggat cggtcgagga catcgcctac
      961 gcggcactgt tcctggcaag cgacgaggcg gcatacatca ctgggcagac catcacggtt
     1021 gacggcggcc agcttttgcc cgagtcgaaa acggcaatgg ggtgatcatg ctcgaaaatc
     1081 tgaaccgcct tgcccaggca tccttgtcgc gctatgagtt gtcgccccgg gccacggcga
     1141 ctctgatcaa ttacacggaa aactcgacct accgggtgga cgaccccgat acggggaacc
     1201 gtttcatttt ccggatctac aggctcggct accatgaccg gcagacgata gaatcggagt
     1261 tgagatggat gagcgcgttg cgtcggaacg caggcgtgca gaccgccgaa accgtctccg
     1321 ccagggacgg cgcgacgctt ctccttttga gcgacgacgt tcttccgcag ccacgatttg
     1381 gcgtcatgtt cactttcctg aacggcaccg agccgccgga ggatcatctt ccggaatctt
     1441 tcgacaggtt gggagaagtc gccgcacgga tgcaccttca ctccaggaca tgggatcttc
     1501 cttccagctt caagcgccct acatggaatt tcgaaagcat gctcgggggc acccgtccga
     1561 tctggggtcg ctggcaggat gggatcgggc tggatcccga gcgtgagaaa ctgctggggc
     1621 gcctgacgga ggtcgtcgcc cggcggttgg agcgattcgg tatcgggcga gagcagttcg
     1681 gcctggtgca cgcggacctg cggctggcga acctgctgct cgatgggaat aagacccagg
     1741 tcattgattt cgacgattgc gggttcggct ggtacctgta cgatctcagc acggcgttga
     1801 cattcatgga ggaacggacg gacgcccaaa agcttgtcgg ttcatgggtc cgcagctacc
     1861 gccggctcgc gcccttgacc aaggatgagg agggggagat cccgaccttc atgatgctcc
     1921 ggaggatgat ggtcgtggcc tggatcggtt cccattccga cacggagttg gctcggagcg
     1981 agggagccgg gtacacgttt ggcacgtgcc gtctggcatc gcggtacctc gatcgcttcg
     2041 catgagaagg ggctgaagga gaaaccggtg cggaagattc tttcgatcaa tgcattcgac
     2101 gcggccagga cggaagctct ggagtccggt atccgggacc tggtggcccg gcggcaccat
     2161 gcgttcgggg cgcatgcgct gttgtcgtat gacaacccgg tt
//
LOCUS       DASSLD010000154         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_160305,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000154 DASSLD010000000
VERSION     DASSLD010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160305"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..1000)
                     /gene="recO"
                     /locus_tag="VFK23_02875"
     CDS             complement(245..1000)
                     /gene="recO"
                     /locus_tag="VFK23_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET7318060.1"
                     /translation="MRSRTEAIIISSMNLGEADRLVTFFSLDRGMLKGVAKNARKSFR
                     RFGAGLEAFTASRLHLYEREHQELVRIESADILTQHPAIAADLGRAAAGAVMLELVKE
                     LAPPGERNAHAYLLLSHVLHLLNEGGDPLFLLGVFEIKFLSLLGYQPRLDHCLSCGTA
                     PRGEIIFLGMKGGILCPDCMVSSGDLQVRLSAGAVGFYYQALRMDMDKVSRLKPSSSI
                     MQELDNAFAAHTFHILGKRLKSTEFLKSVTSLS"
     gene            complement(1004..1591)
                     /locus_tag="VFK23_02880"
     CDS             complement(1004..1591)
                     /locus_tag="VFK23_02880"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HET7318061.1"
                     /translation="MKVLAFNGSPRRGGNTELLLAEAVRGVKEEGGDARVYTLQTLNL
                     KACMNCGHCDSAGRCIIKDDMQAIHDEIRSADRIIVASPIFFFSVSAQTKIMIDRCQA
                     FWAEKYVHKNPVKPGAFGRKGLLLLVGGMKRTEKNVGFACAEATVRAFFRTVNVQEHA
                     TLAYDQVDEKGAIMKHATALSEAYEAGRRLAAKSG"
     gene            complement(1588..2181)
                     /locus_tag="VFK23_02885"
     CDS             complement(1588..2181)
                     /locus_tag="VFK23_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET7318062.1"
                     /translation="MIKTVIFDFGGVLAEEGFREGLLAIAGKYGLDPEAFFADVDSLI
                     EKTGYLTGTTDETSFWNAVRAATGIGGDDGALRREILSRFVLRPEMVAEVDALRAAGL
                     SVSMLSDQTNWLDELDAETALSRHFDRVFNSYRMHKSKRDASVFRDVCAELSAAPGET
                     LFVDDNINHIRRAKSQGLRTIHFTGIDEFKKQLKERL"
     gene            2353..>2628
                     /locus_tag="VFK23_02890"
     CDS             2353..>2628
                     /locus_tag="VFK23_02890"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET7318063.1"
                     /translation="MHDKKEHVLSWIGLSSIPGVGRATFRKLVRHFGSAERSLAASPG
                     ELREVEGLSSKAADAVGAFDWRGPAEREVAKAEEAGVAIVTLDDPSYP"
BASE COUNT          498 a          825 c          779 g          526 t
ORIGIN      
        1 aaggaaggat tctggagaca tcggaaatct tcacggaagc ggaactcaca cacaggctcg
       61 cgcccgggac cgaaaggacg ttttacacga aatacggaga cctcttctcc tacgcctgcg
      121 tgctgctctc ccttctcctg ctcgccaggc tcccgaagaa aagccctgcc ccgtgaccgt
      181 cattccggcg atcgaacggc tgaccaagta cttcatacgc gaaggcattg cctctcccaa
      241 aaccttatga gagggacgtg accgacttca gaaactccgt ggacttgagc cttttcccga
      301 ggatgtggaa ggtgtgggcc gcgaaggcgt tgtcgagctc ctgcatgatg ctcgacgaag
      361 gcttgaggcg ggagaccttg tccatgtcca tcctgagcgc ctggtagtaa aacccgactg
      421 cgcctgcgga gagcctcacc tggaggtctc cggaggagac catgcagtcc gggcagagga
      481 tgcctccctt catgccgagg aagatgattt cgcccctggg cgcggtacca caggagaggc
      541 agtggtccag cctcggctgg tagccgagga gcgacagaaa cttgatctcg aagacgccga
      601 gcagaaaaag cgggtcccct ccttcgttca ggagatggag cacgtgggag aggaggagat
      661 acgcatgggc gtttcgttcg ccgggcggag ccagctcttt taccagttcc agcatgacgg
      721 ccccggccgc cgcccggccc aggtccgcgg cgatcgccgg gtgctgcgtg aggatgtccg
      781 ctgattctat cctgacgagt tcctggtgct cgcgctcgta caggtggagc cgcgaggccg
      841 tgaaggcctc gaggccggcg ccgaagcggc gaaaggactt acgcgcgttc ttggccacgc
      901 ccttcagcat gccgcggtcg agggagaaga aggtaacgag cctgtccgcc tcgcccaggt
      961 tcatgctgct gatgatgatc gcctcggtcc tggatctcat ggattacccg ctcttcgccg
     1021 cgagccttct gcccgcttca taggcctccg agagcgccgt cgcgtgcttc atgatcgcgc
     1081 ccttctcatc gacctgatcg taggcgagtg tcgcatgctc ctggacgttc acggtcctga
     1141 agaacgcgcg caccgtcgcc tcggcacagg cgaaccccac gtttttttcg gttctcttca
     1201 tcccgccgac caggagcaag agtcccttgc ggccgaaggc cccgggtttt acgggattct
     1261 tgtgcacgta tttctcggcc cagaacgcct ggcaccggtc tatcatgatc ttggtctggg
     1321 cgctgacgct gaagaagaag atcggggagg caacgatgat cctgtccgcc gacctgattt
     1381 catcatggat ggcctgcatg tcgtccttga tgatgcagcg gcccgcgctg tcgcagtggc
     1441 cgcagttcat gcacgccttg agattgagcg tttgcagcgt gtatacccgg gcgtcgccac
     1501 cctcctcttt tactccccgg accgcctccg cgagcaggag ttccgtgttc cctccgcgcc
     1561 tcgggctgcc gttgaacgcc agaactttca aagccgctcc ttcaactgtt ttttaaactc
     1621 atcgataccc gtaaaatgga tcgtcctcaa accctggctc tttgcgcgcc tgatgtgatt
     1681 gatgttgtcg tcgacgaaca gcgtttcccc gggggccgcg ctgagttccg cgcacacgtc
     1741 cctgaacacc gacgcgtcac gcttgctctt gtgcattcgg taggaattga acacgcggtc
     1801 aaaatgccgg gacagcgcgg tctccgcgtc gagttcatcg agccagttgg tctggtcgct
     1861 cagcatcgaa accgagagcc ccgcggcgcg aagggcgtcg acttccgcca ccatttccgg
     1921 ccggaggacg aagcgcgaga ggatctcccg ccgaagagcg ccgtcgtctc cgcctatccc
     1981 cgtcgccgct ctcaccgcgt tccagaatga agtttcatcg gtcgttccgg tcaggtagcc
     2041 cgtcttctca atgagcgaat cgacatcggc gaaaaaggcc tcgggatcga gcccgtattt
     2101 cccggcgatc gcgagcaggc cttcccggaa gccctcctcg gcaaggacac cgccgaaatc
     2161 aaagatgacc gtctttatca tggagttcgt cctctgcctt cttccgaagc tcgccgcgcc
     2221 gggcactgct catcgtgcgg ataaaatagc acatttcccg accttctgca aaaaaaactt
     2281 gactatctcc atcgacgctg tataattcga agccttgagc gaaaaacgac ggcatgcgcg
     2341 gcggacgacc acatgcatga caaaaaggag cacgttctct cctggatcgg actctccagc
     2401 attccgggag taggaagagc gacgttcagg aaactcgtca ggcatttcgg ttcggccgag
     2461 cgctcgctcg ccgcatcgcc cggcgagctg cgggaggtcg agggactttc gtccaaggcc
     2521 gccgatgcgg tcggcgcctt cgattggcgc gggcctgcgg agcgggaagt cgcgaaggca
     2581 gaggaagcgg gcgtcgccat cgtcacgctg gacgatccgt cctatccc
//
LOCUS       DASSLD010000155         4076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1160435, whole genome shotgun sequence.
ACCESSION   DASSLD010000155 DASSLD010000000
VERSION     DASSLD010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160435"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VFK23_02895"
     CDS             <1..300
                     /locus_tag="VFK23_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET7318064.1"
                     /translation="DVKDALTAIGIPGMTVIPETRGFGRQKGHVEISHGKEYDIAFVP
                     KVKIEVVVVDAMAEKVLTAIEEKTKTGTIGDGKIFVTTLDDVVRIRTHEHGEPAI"
     gene            539..2353
                     /locus_tag="VFK23_02900"
     CDS             539..2353
                     /locus_tag="VFK23_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HET7318065.1"
                     /translation="MQKIPPSFSSIIRSLGLVFGDIGTSPIYTLTVIFVLLKPTVPNV
                     VGVLSLIVWTLILLVTIQYAWLAMGLSRRGEGGTIVLREILAPLLKSGRQAAFVTLLS
                     YIGISLIIGDGVITPAISILSAVEGMTLIPGFESMPRTTIIIIAALIAFILFAVQKKG
                     TDKVAGSFGPITLVWFLALTASGLASLIHVPSVIKSINPYYALRFLSENGITGFFVLS
                     EVILCATGGEALYADMGHLGREPIVRAWHFVFIALVVNYLGQGAFLIAHPEARNVFFE
                     MVYHQSGFLYIPFLVLSILATVIASQAMISGMFSIVYQGIATRIMPMFKIDYTSMERR
                     SQIYIGSVNWFLLMAVVTIMFEFRQSANLAAAYGLAVTGTMTLTGIMMTWIYGLRRAY
                     GKMAASLLVMLVSATYLVSNTYKIPHGGYWSLIIASLPFSLIMLYRTGQKKLYYSLQP
                     LPLDVFLPSYLQIYRSASKIRGTALFFAKDALEMPPYIVHTIFKNNIIYEDNVIVSVV
                     RREDPFGVTGFFKNDIAPGLRAFEIQIGYMEVPDVEEILREAGINEKTIFYGVEDIIP
                     SNFIWQIFSIIKLLTPNIVQFYKLPPNKLHGVITRVEM"
     gene            2466..2603
                     /locus_tag="VFK23_02905"
     CDS             2466..2603
                     /locus_tag="VFK23_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318066.1"
                     /translation="MNDDTAVKMCDAVKSLNDIKPGSNAQITYYELAGMAVADFVYVP
                     C"
     gene            3052..>4076
                     /locus_tag="VFK23_02910"
     CDS             3052..>4076
                     /locus_tag="VFK23_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET7318067.1"
                     /translation="MAEEAAAPAAAAPAPAPKIDTGDTAWMTVATALVMLMSIPGLAL
                     FYGGLAKRKDSLNTMAMTFVTFCIVSVLWIIYGYSFSFVGDVRGLIGSASRLFLKGIG
                     PNSISDAAKTIPEYIFIAYQLTFAAITVALASGAYIERMKFSAWVIFSIVWMTVVYLP
                     VAHWVWGGGFLAKLGALDFAGGTVVHINAGIAALVGALMLRRRKDKAIIPHNLTLVVT
                     GAGLLWFGWFGFNAGSALAANGLAGAAFINTNTATAMAALAWMFVEWMHSGKPTVLGL
                     ASGAVGGLVAITPAAGFVNIGGALIIGIAAGVIPFFAVAKMKPAFGYDDTLDAFGIHG
                     VGGTIGA"
BASE COUNT          796 a         1202 c         1145 g          933 t
ORIGIN      
        1 gatgtgaagg atgccctgac cgccatcggc atcccgggca tgactgtaat ccccgaaaca
       61 agggggttcg gcaggcagaa ggggcatgtc gagattagcc atggtaagga gtacgatatt
      121 gcctttgtcc ccaaggtgaa gattgaggtc gtggtggtgg acgcgatggc ggaaaaagtc
      181 ctgaccgcta ttgaggaaaa gacaaagacg ggcacgatcg gcgacgggaa gatattcgtc
      241 acgacgctcg acgatgtcgt acggatcagg acgcacgagc acggagaacc ggcgatttga
      301 gaggtcgccg tttatcctcc ctgggccggc atgttccatt gcatgccggc ccttttttta
      361 tttccccgag cactctccag agatcgtttt ctccggaaga cgctccaccc agcgcatcat
      421 cgccgcgccg aagctcagga ttgcggcgcg ggccgggccg acgcgggcgt cggttataat
      481 ggctgtttac tttatcgtgc catatcggta aaataggccg atctgttcac cccgcgccat
      541 gcagaagatc cctccctcat tcagcagtat catcaggtcc ctcggcctcg tgttcggtga
      601 tatcggcacg agcccgatct acacgctcac ggtcatcttt gtgctcctga agcccacggt
      661 cccgaacgtc gtcggcgtcc tctccctgat cgtctggacg ctcatcctgc tcgtcaccat
      721 ccagtatgcc tggctcgcca tgggcctcag caggcgcggc gagggcggta ccatcgtgct
      781 ccgggaaatc ctcgcgccgc tcctcaaatc gggcaggcag gccgcgttcg tcacgctcct
      841 ctcctacatc gggatttccc tcattatcgg cgacggcgtg atcacgcccg ccatcagtat
      901 cctgtccgcg gtcgaaggca tgacgctgat tcccggcttc gaatccatgc cccggacgac
      961 cattattatt atcgccgcgc tgatcgcgtt tattctgttc gccgttcaga agaaaggcac
     1021 agacaaggtc gccgggtcct tcggaccgat aacgctcgtg tggttcctcg cgctgacggc
     1081 ctccgggttg gcgtccctca ttcatgtccc gtcggtgatc aagagcataa acccctacta
     1141 cgcgctccgg ttcctttccg agaacgggat caccgggttc tttgtcctct ccgaggtcat
     1201 tctctgcgca acggggggcg aggccctcta cgcagacatg ggccatctgg gccgggagcc
     1261 catcgtccgg gcgtggcatt tcgtcttcat cgcgctggtc gtcaattatc tcggccaggg
     1321 cgcttttctg attgcccacc cggaggcgag gaacgtcttc ttcgagatgg tctatcacca
     1381 gagcgggttc ctttatatcc cgtttctcgt cctgtccatt ctggccacgg tcatcgcctc
     1441 ccaggccatg atcagcggaa tgttctccat tgtgtaccag ggcatagcca cccggatcat
     1501 gcccatgttc aagatcgatt acacgtcaat ggagcggcgt tcccagatct acatcgggtc
     1561 tgtgaactgg ttccttctga tggccgtggt caccatcatg ttcgagttca ggcagtccgc
     1621 caacctcgcc gctgcctacg ggctggccgt caccgggacc atgaccttga ccgggatcat
     1681 gatgacgtgg atctacgggc tccggagagc atacgggaag atggcggcct cgctcctcgt
     1741 catgctggtt tcggcgacgt acctcgtttc caacacctat aagatccccc acgggggata
     1801 ctggtccctg atcattgcct cgctgccgtt ctcgctcatc atgctgtacc gtaccggtca
     1861 gaagaagctt tattattccc ttcagcctct gccgctcgac gtctttctcc ccagctatct
     1921 gcagatctac cggagcgcga gcaagatcag gggaacggcg ctcttcttcg ccaaggacgc
     1981 gctagagatg ccgccgtata tcgtccatac catattcaag aacaacatta tctacgagga
     2041 caatgtcatc gtgtccgtcg tgcgccggga agatcctttc ggcgtaacgg gctttttcaa
     2101 gaacgacatc gcacccgggc tccgagcctt tgagatacag atcggttaca tggaggtgcc
     2161 ggacgtagag gagatactgc gggaggcggg catcaacgag aagaccattt tctacggcgt
     2221 cgaggacatc attccctcga acttcatctg gcagatattc tcgatcatca agctgcttac
     2281 cccgaacatc gtccaattct acaagctccc cccgaacaag ctccatggcg taataacgag
     2341 ggtggagatg taggagagca tccaggtcct gtacgagaag gtagcagcgg ttgacacgtc
     2401 ccatccaacg ggaagcctga ggctgcattc acggataacc ggagaaccgg gccctgaata
     2461 ccttcgtgaa cgatgataca gccgtgaaga tgtgtgatgc ggtcaagtcg ctgaacgaca
     2521 tcaagcccgg cagcaatgcg cagattacgt attacgaact tgcaggcatg gctgttgcag
     2581 atttcgtcta tgtgccctgc tagtccggtt gccgtgacct gaaaagacgc cgattcttgc
     2641 ggcgtctttt ttttgcggct caagcaccac cccgccggtc aaaacggact cacgaaggga
     2701 tgtttagacc ccccgggttt tcgtgccggt ttcacggtat gagtatgctg ccccatgccc
     2761 aacaattaat catatgctta aaatagcatc cggggaaata gtgcgctgag gtctgcgcat
     2821 cggcccgtgc gtcgcgcagc atctctctca ttcaatgaaa tcaagatgat tggttcttga
     2881 cctccgaaga cttttctctg gcacatgcct tgcttattac cacatgcaga acaattaaac
     2941 acggcatcat caaacaagga ggaatgtact atgaaggtag cagtaatcag attgtttgcc
     3001 gttatggcga tattcctggc gttcgcggcg tttttcgcgc cgggcctgat catggcggag
     3061 gaagctgcag cgcctgcggc agccgcgccc gctcctgcac cgaagatcga caccggagat
     3121 acagcatgga tgaccgtggc caccgcgctg gtcatgctca tgtcgattcc gggtctcgcc
     3181 ctcttttacg gcgggcttgc gaaacgcaag gactcgctca acacaatggc catgactttt
     3241 gtgacattct gcatcgtgag cgtgctgtgg atcatctacg ggtattcatt ttcctttgtc
     3301 ggtgatgtcc gcggtctcat cggcagtgca tcgcggttat tcttgaaagg tatcgggccg
     3361 aacagcattt cggatgcggc aaaaacgatc cccgagtaca tcttcattgc gtaccagctc
     3421 acctttgcgg cgattaccgt ggccctggca agcggcgcct acatcgagcg catgaagttt
     3481 tcggcatggg tcatcttttc cattgtttgg atgacggtgg tgtatctccc ggtcgcccat
     3541 tgggtgtggg gaggcgggtt tttagcaaag ctcggggccc tggatttcgc gggagggacg
     3601 gttgtgcaca tcaatgccgg tatcgcggcc ctggtcggag ctcttatgct taggagacgc
     3661 aaggacaagg ccatcattcc gcacaacctg acactcgttg tgaccggcgc cggccttctg
     3721 tggttcggct ggttcgggtt caacgcaggt tcggcgcttg cggcaaacgg cctggccggc
     3781 gccgccttca tcaacacaaa cacggcgacg gccatggcgg ccctcgcgtg gatgttcgtt
     3841 gaatggatgc actccggcaa gccgacggtc ctcggccttg cctccggcgc ggtcggcgga
     3901 ctggttgcca tcaccccggc tgccggtttt gtgaacatcg gcggggcgct catcatcggc
     3961 atagcggccg gcgtgattcc cttcttcgcg gtggcaaaaa tgaaacccgc attcggttat
     4021 gacgataccc tcgacgcctt cggcatccac ggcgtgggcg gcaccatcgg cgcgat
//
LOCUS       DASSLD010000156         2125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_541934,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000156 DASSLD010000000
VERSION     DASSLD010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541934"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VFK23_02915"
     CDS             complement(<1..259)
                     /locus_tag="VFK23_02915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7318068.1"
                     /translation="MSLAQRDLFIRQIFTTVAPYYSLGTGFSFCFDQLWRRQAVARSG
                     IRAGDRVLDVCTGTGELALLLAQKVGAAGSVTGADFCEEMLE"
     gene            complement(276..1091)
                     /locus_tag="VFK23_02920"
     CDS             complement(276..1091)
                     /locus_tag="VFK23_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318069.1"
                     /translation="MALIKRFNAYHRVLHVVMALSFLGLVASGMPLKYSQAPWAAWLM
                     KLMGGFHAAGLVHRFCAALTFGYFAAHIGYVVYDIAIVRKFKFNPLGPESMVPWIKDI
                     EDILANFRWFFGRGPRPQFDRFTYWEKFDYWAVFWGVGMIGLSGLFLWFPEFFGRFFP
                     GWAFNIATVIHSDEALLAAGFIFTIHFFNTHLRPEKFPMDTVIFTGRLTRRELQHERP
                     LEYQRLTDGNRIDQIETGPQRVWLTDAGIMFGFALVLAGFFLLILILLGQFVY"
     gene            complement(1093..1962)
                     /locus_tag="VFK23_02925"
     CDS             complement(1093..1962)
                     /locus_tag="VFK23_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318070.1"
                     /translation="MKRYIDIPLFEQSVHGFLACAKCHIDVSGFPHKNAARVNCGICH
                     FLGREGAPAEQARAYKLSVHGRASAAGNTAAPACQTCHGSHYIFPPADTRSATNKSKI
                     PALCSQCHPGEFEVYSKSIHGRELLGKNNKKAPTCFDCHLEHLIPPTGDDKWKLDLIK
                     QCGGCHAEQMSTYRKTYHGKVTQLGYTTMAKCSDCHGSHDIVAVKDAASPLSGRNIVA
                     TCRKCHAGATAGFTKFYAHGEEKDRDKYPVLYYTWIFMTTLLISVFAFFLTHTFLWAY
                     RSLKERMNRKGEE"
BASE COUNT          476 a          580 c          645 g          424 t
ORIGIN      
        1 gctccagcat ctcttcgcag aagtctgcgc ccgtgacgga gcccgcggcc ccgaccttct
       61 gcgcgagcag gagcgccagt tcccccgtgc cggtgcagac gtcgagcacg cggtctcccg
      121 cgcggatccc ggaacgggcg acggcctgcc tgcgccagag ctggtcgaag cagaagctga
      181 agccggtgcc gagggaatag tagggagcga ccgtcgtgaa tatctgtctt ataaagagat
      241 cccgctgcgc taatgacatg gcatggcctc cgaaatcaat atacgaattg tcctaaaaga
      301 atcagaatga gcagaaagaa cccggcgagg acgagggcga agccgaacat gatgccggcg
      361 tccgtaagcc aaacccgttg cggtccggtt tcgatctggt cgatcctgtt cccgtctgta
      421 aggcgctgat actcgagggg ccgctcatgc tgcagttccc gcctggtaag tctccccgtg
      481 aaaatgaccg tgtccatggg gaatttttcc ggcctcaggt gcgtgttgaa gaaatgaatg
      541 gtaaagatga aaccggccgc aagaagcgcc tcatcgctgt gaatgacggt ggcgatgttg
      601 aaggcccatc ccgggaagaa gcgtccgaag aactcgggga accagaggaa aagccccgac
      661 aggccgatca tgccgacgcc ccagaacacc gcccagtagt cgaatttttc ccagtaggtg
      721 aaccggtcaa actgcggccg ggggccgcgg ccgaagaacc agcggaaatt cgctaatatg
      781 tcctcgatgt ccttgatcca cgggaccatg gattccggcc ccagggggtt gaatttgaac
      841 tttcttacga tggcgatgtc atacacgaca tacccgatat gggctgcgaa gtaaccgaag
      901 gtgagggcgg cgcagaaccg gtgcacgagc cccgcggcgt gaaatccccc catgagcttc
      961 ataagccagg cggcccacgg cgcctgggaa tacttgagcg gcatgccgga agcgacgagg
     1021 cccagaaagc tcagggccat gacgacgtgc agcacccggt gataagcgtt gaaccgtttg
     1081 atcaatgcca cgctactctt cccctttcct gttcattcgt tccttcagag accggtaggc
     1141 ccacaggaac gtgtgggtca gaaagaacgc gaacacgctt atgagcaggg tcgtcatgaa
     1201 gatccaggta tagtagagga caggatattt gtcccggtct ttctcctcgc cgtgggcgta
     1261 gaatttcgtg aatcccgccg tggccccggc atgacacttt ctgcaggtgg ccacgatgtt
     1321 ccggcccgaa agcggagagg cggcgtcctt cacggcaacg atgtcgtgag acccatggca
     1381 gtcggagcac ttcgccatgg tcgtgtagcc gagctgcgtc accttgccgt gataggtctt
     1441 gcggtaggta gacatctgtt cggcgtggca gcccccgcac tgcttgatca ggtccagctt
     1501 ccacttgtcg tcaccggtcg gaggaatcag gtgctccaga tggcaatcga agcaggtggg
     1561 tgctttcttg ttgtttttgc cgagaagttc ccttccatga atgcttttgc tgtaaacctc
     1621 gaactccccg gggtggcatt gagagcagag cgccgggatt ttcgatttgt tcgtcgccga
     1681 cctcgtatcg gcagggggaa agatgtaatg ggacccatgg caggtctggc aggccggcgc
     1741 cgcggtattt ccagccgccg aagcgcggcc gtggacgctc agcttatacg cccgggcctg
     1801 ttccgcgggt gcgccttccc tgccgaggaa atggcagatc ccgcagttga ccctggccgc
     1861 gttcttgtgg ggaaacccgg agacatcgat atgacacttc gcgcacgcga gaaaaccatg
     1921 gaccgattgt tcgaagaggg ggatatctat gtaacgtttc attccatgtt tgccgtggca
     1981 ttcggaacag gcatccttgg aaaaagcaga cgatgggaaa ataaaacatg agaagatgag
     2041 aacggttatg acgtgcttca atatgcatcg attcatgagt agaattgtag caaagaactg
     2101 tagtctgtca aggaaacgtg caaaa
//
LOCUS       DASSLD010000157         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_247831,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000157 DASSLD010000000
VERSION     DASSLD010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247831"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..>2034
                     /locus_tag="VFK23_02930"
     CDS             35..>2034
                     /locus_tag="VFK23_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET7318071.1"
                     /translation="MRRIHKRMAIHEIESLDDYVAYLHNNPQEIDILFNEFLIRVTSF
                     FRDPDAYKVLAEKALPQIFGGKRDDSSVRIWVPGCSTGEEAYSLAMLCHDYQRGRKGP
                     HRKVQIFATDIDTGAIDIARAGFYPESITVDISPDRLSRHFVKRGGAYRIREEIRDMV
                     VFAVQDIIRDPPFSRLDLVSCRNVLIYMGASLQKRVLALFHYALKADGILFLGSSETV
                     GNAADMYSGLDKKWKIFRALRADRLPMATGDFRRLRAPEERAWQGTPALAAPAANLGD
                     LMGRFLLERYSPPCCVVNDKGDILFIHGKTGKYLEPASGKASLNIIEMAREGIRIEIR
                     TGLRKAATQKKDVVYEGLQVKSNGGYRAVNLEIRYLAKPEPLESLLMVVFNEAAAARG
                     KKTARVKGGPSEKASERLAAMEYELKSTREHLQTTIEELETSNEELKSVNEEFQSANE
                     ELQSSNEELETSREELQATNEELVTVNTELQHKIDELSDSNSDIENLLASTRIATLFL
                     TSDLRIRRFTPSAVDVINIIQTDAGRPIGDLSLKVAYPELVDDAGAVLRTLFQKERSV
                     QQQDGRWFLSRIAPYRTLDGRIDGLVLTFVDITDQKRAEQLEANIVTYLHGIMDTVRE
                     PFMVLDRNLLVISANRAFYDFFRTAPEETEKRPVYELGNGQW"
BASE COUNT          454 a          627 c          604 g          349 t
ORIGIN      
        1 ccacgacttc actctgtaca agcggagcac cgtcatgcgc agaatccaca agcgcatggc
       61 aatccacgag atcgagtcgc tcgacgacta cgtcgcctat ctccacaata atccccagga
      121 gatcgacatt ctcttcaacg agttcctgat acgggtcacg agctttttcc gggaccccga
      181 cgcttacaaa gtgcttgctg aaaaggccct gccgcagatc ttcgggggca aacgcgacga
      241 cagcagcgtg cgcatctggg tgcccggctg ctccacgggt gaggaggcgt actccctcgc
      301 catgctctgc catgattatc agcgggggcg caaaggcccc caccgcaagg tccagatctt
      361 cgccacggac atcgacaccg gcgccatcga tatcgcccgg gcggggttct acccggagag
      421 cataaccgtg gatatcagcc ccgaccggct ttcacggcat ttcgtcaaac ggggcggggc
      481 gtacaggatc agggaagaga tccgggacat ggtggtcttc gcggtccagg acatcatcag
      541 ggacccgccc ttttccagac tcgacctcgt ctcgtgcagg aacgtgctca tatacatggg
      601 cgcctcgctc cagaagcgcg tcctggcctt gtttcactac gccttgaagg ccgacggcat
      661 cctgttcctg ggctcatcgg agaccgtggg caatgccgcg gacatgtact ccggcctgga
      721 caagaagtgg aagatctttc gcgctctgcg cgccgacagg cttcccatgg cgacaggcga
      781 ttttcgccgg ctcagggcgc cggaggaaag ggcatggcaa ggaactcctg cattggccgc
      841 ccccgcggcg aacttaggcg atttaatggg acggttcctt ctggagcgct attcgccgcc
      901 ctgctgcgtg gtgaacgaca agggcgacat cctgttcatt cacggaaaaa ccggcaagta
      961 tctcgaacca gcctcaggca aggcctccct gaacatcatc gagatggcgc gggagggcat
     1021 ccggatcgag atcaggaccg gcctccgcaa ggccgcgacc cagaaaaagg acgtggtcta
     1081 tgagggactt caggtcaaat cgaacggcgg gtaccgggcc gtgaacctcg agatcaggta
     1141 cctggccaag cccgagcccc tcgaaagcct cctgatggtc gtattcaacg aggccgccgc
     1201 ggcccggggg aaaaaaacgg cccgggtgaa aggaggcccc tcggagaagg caagcgagcg
     1261 gcttgcggcc atggaatacg agctcaagtc cacgagggaa catctccaga cgacgatcga
     1321 agaactggag acctcgaatg aagaactgaa gagcgtgaac gaagagtttc agtccgcgaa
     1381 cgaggagctt caaagctcca acgaggagtt ggagacctcg cgggaggagc tccaagccac
     1441 gaacgaggaa ctcgtaacgg tcaacaccga gctccagcac aagatcgacg aactttccga
     1501 ctcgaacagc gacatcgaga accttctggc ctcgacccgg atcgcgaccc tctttctgac
     1561 cagcgacctt cgcatccgcc gcttcacccc cagcgctgtg gacgtcatca atatcatcca
     1621 gacagacgcc ggcaggccca tcggcgacct ttccctgaag gtcgcctacc cggagctggt
     1681 ggacgacgcc ggggccgtgc tccggaccct gttccaaaag gagcgaagcg tacagcagca
     1741 ggacggtcgg tggttcctct cgcggattgc gccttaccgg acgttggacg gtaggatcga
     1801 tggcctggtc cttaccttcg tggacatcac ggaccagaag cgggccgaac agcttgaagc
     1861 gaatatcgtc acatacctgc acggcatcat ggacactgtg cgcgagccct tcatggtgct
     1921 ggacagaaac cttctcgtga tttctgcaaa ccgcgcattc tacgattttt tcaggaccgc
     1981 gcccgaagag accgagaagc ggcctgtcta cgagctgggg aacggccagt ggga
//
LOCUS       DASSLD010000158         3664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1029849, whole genome shotgun sequence.
ACCESSION   DASSLD010000158 DASSLD010000000
VERSION     DASSLD010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029849"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFK23_02935"
     CDS             <1..471
                     /locus_tag="VFK23_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007462595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET7318072.1"
                     /translation="GDAAGYVSDEKASVAACVGATWIAGGVVGTIELLARSLRTGGII
                     LIGEPFWRQLPPTEDVAKGCLAKSISDFLTLPELLASFGHLGYDVVEMVLADQDGWDR
                     YEAAKWLTMRRWLESNPGDEFAKDVRAKLTSERERYAAYTREYLGWGVFALMPR"
     gene            528..2000
                     /locus_tag="VFK23_02940"
     CDS             528..2000
                     /locus_tag="VFK23_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HET7318073.1"
                     /translation="MTLRGRISLILGSLIILLPLAGSAGAASPVFPIDRQFYPYYPSL
                     IRWEKSNAEFTPPGVCRDCHEKQYDEWNGSVHSLAFRDPVYQGELNKAVKAVGHEVSR
                     QCEGCHSAAGMVTGEIKGPGNAGLGEMALAGVSCDICHSISGVTHWQTPSHEPENGSF
                     VLSPGRDDNTGSHLVKRGPGKASADCGGGYHECVESELHVQADLCASCHQVYHYDSHF
                     PIESTYLEWKHGPYAQKDIHCQDCHMVDTDVFRRAADTFQKPQRSEYRHYFNGANYLL
                     AFLAAEAAKRAGDTTLSNTLMKQYEMAVGRLKLAADLEIGPVYQDGKLIEVNVRVRNL
                     RAGHNLPTSLTNIRQMWLEITAKDQDGKVVMSSGTVMPDGSLPGNTRLFNSEGMGDNF
                     HFAIDPWVVTAFSRHETISPRGYKDVRYGTPVTPDVKSISIEAKLRYRQAEQNVAEAL
                     LAAVPKDINLEKSYGLKVVPSLPVVDMVVKEAVFPTLRPR"
     gene            2198..2809
                     /locus_tag="VFK23_02945"
     CDS             2198..2809
                     /locus_tag="VFK23_02945"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET7318074.1"
                     /translation="MTHNLPQLPYAFDALEPYIDAKTMEIHHDKHHGAYVNNLNKALE
                     GHPELQKLTVEELLAQISKVPEAVRTAVRNNGGGHLNHSMFWKIMKKGGGGEPSGDLA
                     DAIKKTFGNFGDFRKTFTQAATTRFGSGWAWLLIRGGKITVESTANQDNPIMDGGKAI
                     FGLDVWEHAYYLKYQNRRPEYIEAWWNTVNWAQVADNFAKAKA"
     gene            complement(2946..3446)
                     /locus_tag="VFK23_02950"
     CDS             complement(2946..3446)
                     /locus_tag="VFK23_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318075.1"
                     /translation="MATEKQKEAAKNNIKKAQAAWKAMSSTQHARSQPEGRARKKPGT
                     TGEGKYYRIVVRSKEDFVTFRYHDVGEPGGIQRLAGKRSSGSWDDQAWLISKEMAHTE
                     GNTLVADTPDAKKILQVIGPAKHVKGDIFQGHPRKNIPEREKPTPAQRRARMENIRKA
                     QQARRK"
     gene            complement(3461..3604)
                     /locus_tag="VFK23_02955"
     CDS             complement(3461..3604)
                     /locus_tag="VFK23_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HET7318076.1"
                     /translation="MTISVSHLQPVAALIAGILILVIPRLLNYIVAIYLIIIGILGLT
                     HNM"
BASE COUNT          784 a         1006 c         1023 g          851 t
ORIGIN      
        1 ggcgatgctg ccggctatgt ctctgacgag aaggccagtg tggcagcctg tgtcggtgcc
       61 acttggatcg ccgggggagt cgtcggcact atcgagctac tggcgcggag cctgcgtacc
      121 ggagggatca tcctcatcgg cgagcccttc tggcggcagt taccgccgac ggaagatgtt
      181 gccaaggggt gtcttgccaa gtcaatctcc gactttctca cgcttccaga acttctcgcg
      241 tctttcgggc accttggcta cgacgttgtt gaaatggttc tggctgatca agacggctgg
      301 gacagatacg aggcggccaa atggctcacc atgcgccgat ggcttgaatc caatcccggc
      361 gacgagttcg cgaaagatgt tcgagccaaa ctgacctcgg aacgcgagcg ctacgccgct
      421 tacacgcgtg aatacctggg ctggggtgtg ttcgcgctga tgccgcggtg atgcgttggc
      481 gcatccggct ggataaccca ttttcggaaa cgcgaaagga gggttccatg accctgaggg
      541 gaagaatttc tttgattctc ggatcactca tcattctcct cccgctggcg gggtccgccg
      601 gtgcggcttc ccctgttttc cctatagaca ggcaatttta tccttactac ccgtcgctca
      661 tacggtggga aaaatcaaat gcggagttca caccgcctgg cgtctgcaga gattgccatg
      721 aaaagcagta cgacgaatgg aacggctccg tccatagcct ggcgtttcgg gatcccgtgt
      781 atcaaggcga gctgaacaag gcggtaaagg ccgtcggaca tgaggtttcc aggcaatgcg
      841 agggatgcca ttccgcggcc gggatggtaa cgggagagat caaagggccg ggaaatgccg
      901 gcttggggga aatggctctg gccggggttt cctgcgatat ctgtcattcc atcagcgggg
      961 ttacgcactg gcagacccca tcccatgaac ccgagaacgg ttccttcgtc ctttcaccgg
     1021 ggcgcgacga caatacgggg agtcatcttg tcaaacgagg ccccggaaag gcatccgcgg
     1081 attgtggcgg gggataccac gaatgtgtcg aatcggaact tcacgttcag gcggatcttt
     1141 gcgcctcctg tcatcaggta taccattacg attcccattt ccccatcgag tcgacctatc
     1201 tcgaatggaa gcacggaccg tatgcccaga aggacatcca ctgccaggac tgccatatgg
     1261 tggatacgga tgtgttcagg cgcgccgccg atacgttcca aaagccgcaa cgaagcgaat
     1321 accgccacta tttcaacggt gcgaattatc tgctcgcgtt tctcgccgcg gaagccgcca
     1381 agcgggcggg tgatacgaca ctgtcgaata cgttaatgaa acagtacgag atggcggtcg
     1441 ggagactgaa gctggcggcg gacctcgaaa tcgggcccgt atatcaagac ggcaagttga
     1501 tcgaggtgaa cgtacgggtg aggaatttgc gtgccggtca caaccttcct acctcgctca
     1561 ccaacattcg ccagatgtgg cttgaaatca ctgcgaaaga ccaggatggg aaagtcgtca
     1621 tgagcagcgg aacagtcatg ccggatggca gtctgccggg caacactcgt ctcttcaatt
     1681 cggaagggat gggagacaac tttcacttcg ctatcgatcc gtgggtcgta accgcctttt
     1741 cacgccacga gacgatctct ccgaggggat acaaggatgt ccgctacgga acccccgtga
     1801 cgccggacgt caagtcgatt tccatcgagg cgaaacttcg ttaccgtcaa gcggagcaga
     1861 atgtggccga ggcattgctg gcagccgttc ccaaggacat caatcttgaa aaatcctacg
     1921 gattgaaggt ggttccgagc cttccggtgg tggatatggt ggtgaaagag gctgtattcc
     1981 cgactctgcg accccgttag accttccgtc gtcagaaagg gagcccgtcc aaaccggtgc
     2041 tccctttttc tttgcgtcat gggtgctgat ttagctcctg gaatgacccc atggcttatg
     2101 gcggacaaat cctcgcgcgc ggaaacgatg ccgcgttctc gacgcatctt atcttgcaga
     2161 tgattccggt cacgaaccgg caggaaagga gatcccgatg acgcataatt tgccgcagct
     2221 cccatacgcc ttcgacgcgc tggaaccgta catcgacgcg aagacgatgg agattcatca
     2281 cgacaaacac cacggggcgt atgtcaacaa tcttaataag gctctcgaag gccatcccga
     2341 gcttcaaaaa cttacggtcg aggagctgct cgcacagatc agcaaagtgc cggaagccgt
     2401 tcgtactgcc gtgcgcaaca acggcggcgg ccatctgaat cattccatgt tctggaagat
     2461 catgaaaaag ggcggcggcg gtgagccgtc gggggatctg gccgacgcca tcaaaaagac
     2521 gtttggcaac ttcggggatt tcaggaaaac ctttacccag gccgcaacaa cgcgattcgg
     2581 gtccggttgg gcatggttgc tgatccgtgg aggaaagatc accgtcgaat cgaccgcaaa
     2641 ccaggacaat cccatcatgg acggcggcaa ggcgatcttc ggcctggacg tctgggagca
     2701 cgcctattac ttgaagtatc agaatcgacg ccccgagtac atcgaggcat ggtggaacac
     2761 cgtcaattgg gcgcaggtcg cggacaactt cgcgaaggcc aaggcgtaag tttcgttcat
     2821 tgcaatagca aaagggaact tggcttcgcg ccaagttccc tttttacgtc tttcccctga
     2881 gcggctgatt tctgtcccgc agcatggaca ctcttgccgc agatcgtcac atgactctct
     2941 ctatctcact tccggcgcgc ctgttgggct ttgcgaatat tttccattcg cgcgcgtctt
     3001 tgagcagggg tcggtttttc cctttcagga atattttttc tgggatgtcc ctggaagatg
     3061 tctcctttaa catgtttcgc aggtccgatc acctgcaata tctttttcgc atcgggagta
     3121 tcggcgacaa gcgtgtttcc ttccgtgtga gccatttcct tgctgatcaa ccaagcctga
     3181 tcgtcccagg agccgctgga tcttttcccc gccaatctct ggatccctcc cggttctccc
     3241 acatcatggt atctgaaggt gacaaagtcc tctttcgatc gaacgacgat gcgataatat
     3301 tttccttctc ccgttgtccc cggttttttt cttgcccgtc cctccggttg acttcttgcg
     3361 tgctgcgtcg atgacattgc tttccatgcc gcctgtgctt tctttatatt atttttcgca
     3421 gcttcctttt gcttttccgt agccattggt ttttcctccc ttacatattg tgagtcagtc
     3481 cgaggattcc aatgatgatg agatagatgg cgacgatata attcaaaagt ctcggaatga
     3541 cgaggatcag gattccggcg atcaaggccg ctacaggttg taaatgagag acgctgatgg
     3601 tcatgccgat tccgcctcct ttatgggatt tccctttccc tcccggtaag acgagcatcc
     3661 gggg
//
LOCUS       DASSLD010000159         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_410158,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000159 DASSLD010000000
VERSION     DASSLD010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410158"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFK23_02960"
     CDS             complement(<1..245)
                     /locus_tag="VFK23_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318077.1"
                     /translation="MGKRQLLFVTYRDENLEEGVSYAIELAKAMFEDITLLLVRKKSN
                     LMDKLGNLMTAVTFAEADEHDTARQILSGESVSPERH"
     gene            656..1267
                     /locus_tag="VFK23_02965"
     CDS             656..1267
                     /locus_tag="VFK23_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HET7318078.1"
                     /translation="MTEANNPGEEKAWQILASMSPEAVCRAADTSYNADTSSYTIKSL
                     GMDVVVSVPQRSMTSASDGSKVLLQRLGYFFRLSILWYLVSVKDVACTGTPVKLENIP
                     GGDIFTRGSHVLPLDKVAQKYGRDKSGFIGKGKRLGGEIVTGGDASIRLLPLPRIPVV
                     MTLWLEDEEFPPRVDLFFDTTCSMQMPVDMLWSVAMMSILIML"
     gene            complement(1371..1670)
                     /locus_tag="VFK23_02970"
     CDS             complement(1371..1670)
                     /locus_tag="VFK23_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318079.1"
                     /translation="MSDKGLRKAVLIGAVIGALVTLGIALSMDLFFADTLQGSWRDAA
                     AKDVTRMFGPACGQNVFAVMLVLAVVMAFLAGFGAMMGAAAGLIMNRFFKMLLKS"
     gene            complement(1721..>2281)
                     /locus_tag="VFK23_02975"
     CDS             complement(1721..>2281)
                     /locus_tag="VFK23_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318080.1"
                     /translation="PAKGDRAVFEELCYCILTAGSSAKMGMRTVEALRDFLHTGSLKE
                     LQARARAGRVRFWRVRPSYIVATREYLRSTCGMKLKKTLASITCPDARRDFLAKNKRI
                     KGIGYKEASHFLRNIGYSGYAILDKHIVNSLCDLGVIKTRKPPSGRDEYLTIERSMMR
                     FAKKLGIDMDHLDLLLWSRKTGEILK"
BASE COUNT          520 a          618 c          629 g          514 t
ORIGIN      
        1 tcgtgccgct ccggactgac ggattcgcct gacaggatct ggcgcgccgt atcatgctca
       61 tcggcttcgg caaacgtcac ggcagtcatg aggtttccaa gcttgtccat aaggttgctt
      121 ttcttccgga ccagaaggag cgtaatgtcc tcaaacatcg ccttggccag ttcgatggcg
      181 tacgatacgc cctcttccag gttctcatcc cggtaggtga cgaacaacaa ctgtctcttt
      241 cccattgtga tgacctccta taaattcgat aactgacata cttaatagca actgagatgc
      301 catcttaaaa cacgttgttt ttacggaata aaattcaata gagaaattag ctgctgtttc
      361 aaatttgaac ggcactgaaa caccatgagg taggcgggct gaagactaag gcgcgatgaa
      421 acgacattag gccttgtttg caggcataaa ctcaaaagga tctactataa tgagaaacac
      481 tcctgatcat tattcctgac cttgactgtt tcaatatgga acgggaatcc gctttgcaat
      541 cggggaaata tctggtagga tgaaaaaaat cgtgaatcgg ttcgaaatat agatattcag
      601 gtacgaccaa agaaggatat gcaacgaata ccatgacaaa tttaaagagg caagaatgac
      661 tgaagcgaac aatcccggtg aagaaaaggc gtggcagatc ctggcctcga tgagtcccga
      721 agcagtttgc cgggctgcgg atacatccta taacgcggac acttcctcgt acaccatcaa
      781 atccctgggg atggatgttg tcgtgtccgt tccacagagg tccatgacaa gtgcctccga
      841 cggcagcaag gtcctcctcc agaggctcgg ctattttttc cgtctctcga ttctctggta
      901 tctcgtgagc gtcaaggacg tcgcctgcac aggcacgccc gtcaagctcg agaacatccc
      961 ggggggcgac atcttcacca ggggatcgca cgttttgccg ctcgacaagg tggcgcagaa
     1021 atacggaagg gacaagtcag gattcatcgg gaaaggaaaa cgcctcggcg gggaaatcgt
     1081 aacgggggga gatgcctcaa tccgtctctt accgttgccg cggataccgg tcgtcatgac
     1141 gctctggctg gaggacgagg agttcccccc gcgcgtggac ctgttttttg atacgacctg
     1201 cagtatgcag atgcccgtgg atatgctctg gtccgtcgcg atgatgagca tcctgatcat
     1261 gttgtgaaat ccgctcgtca gaaccgggtc ttctcgacga ttttagggtg ggagctgtcc
     1321 gggggaaggg ggaatgtgtg gcgggagtct cactgccccg tccgggaaca tcatgatttg
     1381 agcaacattt tgaaaaatct gttcatgatg aggccggccg cggcccccat catcgcgccg
     1441 aaaccggcca ggaacgccat gacaaccgcc aggacgagca tcacggcgaa gacgttctgg
     1501 ccgcaggccg ggccgaacat cctggtcacg tctttagccg ccgcgtcgcg ccaggatccc
     1561 tggagggtat cggcgaaaaa aaggtccatg gaaagggcga tgcccagcgt aacgagggcg
     1621 ccgataaccg cgccgatgag aacggccttc ctgagtccct tgtcgctcat gggatcacct
     1681 tacgtttgat attccgcatt ccgcattccg aattcagcaa ttacttcagt atctcccctg
     1741 tctttcgcga ccacaggagc aggtccaggt ggtccatgtc gatgccgagt tttttcgcaa
     1801 accgcatcat gctccgttct atcgtgaggt actcgtcgcg tcccgagggc ggcttccgtg
     1861 tcttgatcac gcccagatcg cagaggctgt tcacgatatg cttgtccaat atcgcatagc
     1921 cggagtagcc gatgttccgg agaaaatggc tcgcctcctt gtagccgatg ccttttatcc
     1981 ttttgttctt cgccaggaaa tcgcggcggg cgtcggggca ggtgatggat gccagggttt
     2041 ttttgagttt catgccgcag gtggaacgaa ggtactcccg ggtcgcgacg atgtaggacg
     2101 gccgcacgcg ccagaaccgc acgcggcccg cccgtgccct cgcctgaagc tccttgaggc
     2161 tccccgtatg cagaaaatcc ctgagcgctt cgaccgttcg catgcccatc ttggcgctgc
     2221 tgccggcggt cagaatgcag taacagagtt cttcgaagac cgcccggtcg cccttcgcag
     2281 g
//
LOCUS       DASSLD010000160         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_768979,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000160 DASSLD010000000
VERSION     DASSLD010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768979"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="VFK23_02980"
     CDS             <1..1271
                     /locus_tag="VFK23_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET7318081.1"
                     /translation="GTYANLLTYNGAYPAPTIRVKRGDTLKVRLKNSLHPMMGQNILG
                     HDRNMTNLHTHGLHVSPSGNADNMMVMLMSGDTFDYEYDLSLQEPGTINFYHPHIHGS
                     VAEQYWGGMAGALLVEDGIQALAGFETHTLFIKDITLSGGAPEPYTSLMDYMHGKEGN
                     TVMVNGQVNPVLSIRPGQVQRWRVVNACNARFLKLSLEGHTLQIVGTEGGLLKNPYPL
                     SSILLSPGERLDILVKANQTAKNYRLLSLAYNRGGGSMGQQVTLMTLSCKGSRANDAV
                     PSLINPGAVRVDPVVAKTERVALSMGQGKGYVNGISFTMENYYSIHSHLGTHEVWEIV
                     NQSGMDHPFHQHVNPCQVLSVTGGDAAYASFLTKTPAWKDVVVIPKMGSAKILVPVMD
                     YAGMAMFHCHIVEHEDIGMMGMWHIMDMPM"
     gene            complement(1324..1791)
                     /locus_tag="VFK23_02985"
     CDS             complement(1324..1791)
                     /locus_tag="VFK23_02985"
                     /inference="COORDINATES: protein motif:HMM:NF025825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET7318082.1"
                     /translation="MFWSFSFHLMPDETILEDSTSRSLAILKSTYSVFLTNKRVIFRF
                     DGMGSSLAQSFLYREIDKVQPSKRLMINYLCVQTSGEQYFLHTPEPAYWAERILKMKK
                     SASQVPEVSRPAEPAGRTKKQDLVEMLTTLRKYNLLTDAELAGKIKLLEKQQL"
     gene            complement(1917..>2289)
                     /locus_tag="VFK23_02990"
     CDS             complement(1917..>2289)
                     /locus_tag="VFK23_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318083.1"
                     /translation="NDLAALAGFGIGIRGADANGVDVGVSAGIRKYLKVDDFAPVVGG
                     VFFYSNTKDGDSQDVSVMGEAGAEYFLHKQFSIEGKVGVGYVSRESKTTTTQGGFITT
                     TTRRESDLGTERLGISFNFYF"
BASE COUNT          515 a          674 c          643 g          457 t
ORIGIN      
        1 acggaaccta tgcgaacctc cttacctata atggagccta tcccgctccc acgatccgcg
       61 taaaaagagg cgataccctg aaggtgcgtc tcaaaaactc cctgcacccc atgatggggc
      121 agaacatcct gggccacgac cggaatatga cgaatctcca tacccacggc ctgcacgtat
      181 ccccgtcggg gaacgcggac aatatgatgg tcatgctcat gtcgggagac accttcgatt
      241 acgaatacga cctctcgctg caggagcccg gaacgatcaa tttctaccat ccccacatcc
      301 acggatcggt ggcggagcag tactggggcg gaatggctgg cgcccttctg gtcgaagacg
      361 gcatccaggc cctggcggga ttcgagacgc acaccctttt catcaaggac atcacgctct
      421 cgggcggggc gcccgagccc tacacatcgc tcatggatta catgcacggc aaggaaggaa
      481 acaccgtgat ggtaaacggg caggtaaatc ccgtgctctc catcaggcca ggccaggtcc
      541 agcgctggcg cgtcgtaaac gcctgcaacg cccggttcct gaaactgagc ctcgagggcc
      601 acacgctcca gatcgtcgga accgaaggag gccttctgaa aaacccctat cccctgtcgt
      661 cgatcctgct ttcaccgggc gagcgtctgg acatcctggt gaaagccaac cagacggcga
      721 agaactaccg gctcctttcc cttgcctaca accgcggcgg cggaagcatg ggccagcagg
      781 tgacactcat gacactttcc tgcaagggca gccgcgcaaa tgacgccgtc ccctcgctca
      841 tcaaccccgg cgccgtgcgc gtcgaccccg tagtcgcaaa gaccgaacgg gtcgcgctgt
      901 cgatggggca gggaaaaggg tatgtgaacg gcatatcctt taccatggag aactactatt
      961 ccatccattc tcatctcggg acgcatgagg tctgggagat cgtgaatcag agcgggatgg
     1021 accatccctt ccaccagcac gtgaacccgt gccaggtgct gtccgtgacc ggcggcgacg
     1081 cagcctacgc ttcgtttctg acgaaaacgc cggcatggaa ggacgtggtc gtcatcccta
     1141 aaatggggag tgcgaagatc ctcgtcccgg tcatggacta tgcgggaatg gcgatgttcc
     1201 actgccacat cgtcgagcac gaggacatcg ggatgatggg catgtggcac atcatggaca
     1261 tgccgatgta acagcccaaa caaggagtga tgcggaaaga tcgtttcaga aagccggtac
     1321 ggttcaaagc tgctgctttt cgagcagctt tatttttccc gccagctccg catcggtgag
     1381 gaggttgtat ttccggaggg tcgtaagcat ctcgacgagg tcctgctttt tcgtccggcc
     1441 tgccggttcg gccggccggg agacttccgg gacctgggat gcgctcttct tcattttcag
     1501 aattcgttcc gcccagtacg cgggttcagg cgtgtggagg aaatattgtt cgccggatgt
     1561 ctggacgcag agatagttga tcatgagcct cttcgaaggc tgaaccttgt cgatctcacg
     1621 atagaggaac gactgcgcca gtgaactccc catcccgtcg aaacggaaaa tgaccctctt
     1681 gttcgtgaga aagacggagt acgtggattt caggattgca agagaccggc tcgttgaatc
     1741 ttcaaggatg gtttcatcgg gcatcaggtg aaaggaaaag gaccagaaca tatcaaaaaa
     1801 gacctccccg ggagattcgg gtctccggaa gtgctgttca gtccgtaaag aggacctgca
     1861 taaatccccc ggcggtcagt cgccggggga atgtgacacg atgaaaaagg agggcgctag
     1921 aaataaaaat tgaagctgat gccgagcctc tcggttccga ggtcggactc cctcctcgtg
     1981 gtcgtcgtaa tgaaaccgcc ctgggtcgtc gtcgtctttg attcccgtga aacataaccg
     2041 acaccgactt ttccctcgat gctgaactgc ttatgcagga aatattccgc gccggcctcc
     2101 cccattacgc tgacgtcttg cgagtccccg tccttcgtat tgctgtagaa gaacacgccg
     2161 ccgacaacgg gcgcgaagtc gtccaccttc aggtatttcc tgattccggc ggacacaccg
     2221 acatcgactc cgttggcgtc agcgccccgt atgcctatgc cgaaacccgc gagcgcggcg
     2281 agatcgttc
//
LOCUS       DASSLD010000161         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1263747, whole genome shotgun sequence.
ACCESSION   DASSLD010000161 DASSLD010000000
VERSION     DASSLD010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263747"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..>2098
                     /locus_tag="VFK23_02995"
     CDS             346..>2098
                     /locus_tag="VFK23_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318084.1"
                     /translation="MQIVTLPYGKTGWSERTRIVDEILSSRATPPRIYDDVLILVPSS
                     RLKRAYGALFLDRIGRLFGNVSLVPPQVLTLYQFIRLLSAGLTGATLIDENSRLVLLE
                     GIVKGLMSGESAFGKNPDVLAPALSAAVADMIEQLTSAGVSAERLSEAVAESDFPDKA
                     SVRLLTRAFRRYEDLLAAKGLVDPDGLLSLITESFDTALISPYSRIVIDGLPDGGRLQ
                     SLLLGKIAANADCTVFIEAPSSESVMQAGLSHPLRLTREFLEDRGLAPGAASPGMTED
                     DLYVSGTLFSDRPFAEALERAPSVRSFRKRINLLSAINPREEATLIAREVKTTVRNGT
                     APGSILVAFPALDEYGPLIEEIFTDYGIPYNRALGRQLSASPVATAVISLLRACQEDF
                     SAPSLMRVMSSPLLKFGGQPSLAPALDRLLRDRRITGGKGKILQTLRHHASEEGGLDV
                     LAEALNDLFAALLPFEAQEPAPLSSWMERLDSLIAWAEVGGRVAAVKGPLNINLQAYR
                     KLSETLDSLDRAGRLFPEYRYTFREWLFLLRKTFAHERFQVPPEDEGGVQILGLGESA
                     GHEWSEIYLGGLIDGKFP"
BASE COUNT          419 a          656 c          576 g          447 t
ORIGIN      
        1 attcattgtc aataattatt acctcggccg gtcaacgtaa aacgctattt tattctgata
       61 aacgcgatga taaataatac ttcagcgaat cttaattgtc attcctgcga aagcaggaat
      121 ccattttcag aaagaggcaa aaaaaccaat ggatacccgc ttgctcgggt atgacaggtg
      181 gagttacgat gctttttttt tcgtatagta tctccctgta taaaagtttc gtcgcaggtg
      241 ataagagatg ttctatatcg tctctgagag atcattatat cgcttgtctc acttgtaatg
      301 ctaagcattg tctctttcgc acacttgtgg cataatcatc acacgatgca gatcgtcacc
      361 ctgccatacg gcaagaccgg atggagcgaa agaacgcgta tcgtcgacga gattctctcg
      421 tcccgagcga caccgccccg catctacgat gacgtcctca ttctcgtgcc ctcctcgcgt
      481 ctgaagcggg cctacggcgc tctctttctc gaccggatcg ggaggctttt tggaaacgtt
      541 tcgctcgtcc cgccccaggt actgaccctc taccagttca tacgccttct ctccgcgggt
      601 ctgaccggag cgacgctcat agacgagaac agcaggctcg tgctgctcga agggatcgtc
      661 aagggtctga tgagcggcga gtccgccttc ggcaaaaatc cagacgtcct cgccccggcc
      721 ctttccgcag ccgtggcgga catgatcgaa cagctcacct cggcaggggt ctccgcggag
      781 cggctctccg aagcggtcgc agaaagcgat ttcccggaca aggcctcggt gaggctgctt
      841 acgcgcgcct tccgcagata cgaggacctg cttgccgcaa aaggcctcgt ggaccccgac
      901 ggtctgcttt ccctgatcac ggaaagtttc gacaccgccc taatctcccc gtactcccga
      961 atcgttattg acgggctccc cgatggagga aggctccagt cgctgctgct cgggaagatc
     1021 gccgcgaacg cagactgcac cgttttcatc gaggcgccat cttccgaaag cgtgatgcaa
     1081 gccggtctct cccaccccct ccggctcacc agggaatttc ttgaggacag ggggcttgcc
     1141 ccgggggctg catcgcctgg catgaccgag gacgatttgt acgtctccgg gacgctcttc
     1201 tcggaccggc cttttgccga ggcgctggaa agggcgccct ccgtccggtc cttccggaaa
     1261 cggatcaacc ttctctccgc gatcaatccc cgcgaggagg cgaccctgat cgcccgggaa
     1321 gtgaaaacaa ccgtccggaa cggcactgcg cccggttcca tcctcgtggc ctttcccgcg
     1381 ctcgatgaat acggtccgct catcgaagag atcttcacgg actacggcat cccttacaac
     1441 cgggcgctcg gaagacagct cagcgcatcg ccggtcgcca cggccgtgat ctcgctttta
     1501 cgggcctgcc aggaagactt ctcggcgcct tccctcatga gggttatgag ttcgcccttg
     1561 ctcaaattcg gcgggcaacc gtcccttgcg ccggcgctcg accgcctctt gcgggacaga
     1621 cgtataaccg gcggcaaagg caaaatcctc cagacactca ggcatcatgc gagcgaagaa
     1681 ggcgggttgg atgtcctcgc ggaagcctta aacgacctgt tcgccgccct tctgcccttc
     1741 gaagcacagg aacccgcccc gctctcatcc tggatggagc ggctcgacag ccttatcgcc
     1801 tgggcggagg tcggcggtcg ggtggcagcc gtcaagggac cgctcaacat caatctccag
     1861 gcgtaccgga agctctctga gactctggac tctctggacc gtgcgggccg gctctttccg
     1921 gaataccgtt acaccttccg ggaatggctc ttcctgctca ggaagacctt tgcccatgaa
     1981 cggttccagg tccccccgga ggacgagggg ggcgtccaga tactgggcct cggagagagc
     2041 gcgggccatg agtggagcga gatctatctc ggcgggttga tcgacgggaa attccccc
//
LOCUS       DASSLD010000162         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1518498, whole genome shotgun sequence.
ACCESSION   DASSLD010000162 DASSLD010000000
VERSION     DASSLD010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518498"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VFK23_03000"
     CDS             <1..969
                     /locus_tag="VFK23_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HET7318085.1"
                     /translation="RKCRVVTRFRNTIGLEGRLSVRLQPNHPTDDPKGIAASILDGLF
                     YGAGDAVIGVNPATDNIGNAVVLLEMLDSLREKYDIPVQSCVLAHVTTTMEAIRRKAP
                     VDLVFQSVGGTEAVNKSFGVNLAMLEEARRMALELKRGTVGDNVMYFETGQGSALSAN
                     AHHGADQQTLEARAYGVARKFEPLLVNTVVGFIGPEYLYNGKQITRAGLEDHFCGKLL
                     GLPMGADVCYTNHADADQDDMDGLLTLLAVAGCNYIMGVPGADDIMLGYQSTSFHDAL
                     YLRKVLGLRPAPEFEEWLVRMNLVDSNLMPKIPPPDHRLLEYLGGV"
     gene            1115..1879
                     /gene="eutC"
                     /locus_tag="VFK23_03005"
     CDS             1115..1879
                     /gene="eutC"
                     /locus_tag="VFK23_03005"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HET7318086.1"
                     /translation="MEKMVYDDPWISLRKYTKARIALGRAGTALTTAELLRFRLDHAR
                     ARDAVLKDMDPSVVIRSLEKFQMPVPELTTRAHDRNEYIHRPDLGRRLSPESAALLAK
                     HRGDYDIALMIADGLSGIGVETNAGPFLDCLLALLRDAHYTLAPLCIIRQGRVAVSDE
                     VGSILGAKLSAILIGERPGLSSPDSMGIYMTFRPEPGLTDERRNCISNIRSEGLAWPA
                     AARKLMYFIRESLRLKLSGVKLKDDEDRDRGRLIEA"
     gene            complement(1908..2297)
                     /locus_tag="VFK23_03010"
     CDS             complement(1908..2297)
                     /locus_tag="VFK23_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET7318087.1"
                     /translation="MQQKRVTRWYRLLLAISLGVIFYLATTDREIPGVEDVNDKVEHV
                     FAFSVLALLADFSWPESGFGAVKILSLLAYGLAIEITQYFIPYRECSLFDLGADAVGL
                     LLYGLMVPLLMKVNWLKGRWRDEKPPL"
     gene            complement(2302..>2630)
                     /locus_tag="VFK23_03015"
     CDS             complement(2302..>2630)
                     /locus_tag="VFK23_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HET7318088.1"
                     /translation="AGAAMLTGSGRVSGIRREEGIAAGRRRKTAGAGELDRALFERLR
                     ALRKRIAEDNHVPPYIIFSDKTLHEMCRHFPVTADSMRRIAGVGDVKLERYGGKFIAE
                     IKAYRG"
BASE COUNT          563 a          811 c          770 g          486 t
ORIGIN      
        1 aggaaatgca gggtcgtgac caggttcaga aataccatcg gcctcgaagg ccgcctgtcc
       61 gtgagactcc agcccaacca tcccaccgac gacccgaagg gaatcgccgc gagtatcctg
      121 gacgggcttt tttacggcgc cggcgacgcc gtcatcgggg tcaatcccgc gactgataat
      181 atcggcaacg cggtcgtctt gctggagatg ctcgactccc tcagagaaaa atacgacatc
      241 cccgtccagt cctgcgtcct cgcccacgtt acgacgacca tggaggcgat caggcgcaag
      301 gcgcccgtgg accttgtctt tcaatccgtc ggcggaacgg aagcagtcaa taaaagtttc
      361 ggcgtaaatc tcgcgatgct cgaggaggcc cgccgcatgg ccctggaact gaagcgcggg
      421 accgtcggcg acaacgtcat gtacttcgag accggacagg gaagcgcact ctccgcgaat
      481 gcgcatcacg gggccgacca gcagaccctg gaggcaaggg cctacggcgt cgcaagaaaa
      541 ttcgagcccc tgctcgtcaa cacggtcgtc ggtttcatcg gtcccgaata cctgtacaac
      601 ggcaagcaga tcacccgggc ggggctggaa gatcatttct gcgggaaact gctcggcctt
      661 cccatggggg cggacgtctg ctacacgaac catgcggacg ccgaccagga cgacatggac
      721 ggcctcctga ccctgctcgc ggtggccggc tgcaactaca tcatgggcgt gcccggcgcg
      781 gacgacatca tgctcggcta ccagagcacg tccttccatg acgcgcttta tctccggaag
      841 gtgctgggac tccggcccgc accggagttc gaggagtggc tcgtcaggat gaacctggtg
      901 gattcgaacc tgatgccaaa gatcccgccg ccggatcatc gtcttctgga atatttgggg
      961 ggcgtataat attttacgtt attcaggttg tcattcccgc ccctgcttca atcatgctgg
     1021 ggcaggctcc aacgggaatc cactggcttt ttaaaaagac taaattcaaa atggattccc
     1081 gatagaagca ttcgggaatg acggattggt acacatggaa aagatggtat acgacgaccc
     1141 ctggatatca ctgcggaagt acacgaaggc caggattgcg ctgggaaggg ccggtaccgc
     1201 actcacgacc gcggaactgc tcaggttcag actcgaccat gcgcgggcgc gggacgcggt
     1261 attaaaagac atggacccgt ccgtggtcat aaggtctctg gaaaaattcc agatgcccgt
     1321 cccggaactg acgacacggg cacacgaccg gaacgagtac atccacaggc ccgatctcgg
     1381 cagacggctt tcgccggaat cggctgcgct gctcgcaaaa caccgcgggg actacgacat
     1441 cgccctcatg atcgccgacg gactttcggg gattggtgtg gaaacaaacg ccggaccttt
     1501 cctggattgc ctcctggctc tcctgcggga cgctcactac acgctcgcgc ctctgtgcat
     1561 catccgtcag ggcagagtgg ccgtcagcga cgaggtcggg agcatcctcg gggcgaaact
     1621 gagcgcgatc ctgatagggg aaaggccggg cctgagctcg cccgacagca tggggattta
     1681 catgaccttt cgccccgaac cgggccttac cgacgaaagg cgaaactgca tatcgaacat
     1741 caggtccgaa ggtctcgcct ggcccgcggc cgcacggaag ctcatgtatt tcatcaggga
     1801 gtccctgcgc ctcaagctct ccggcgtgaa gctcaaggac gatgaagatc gcgatcgggg
     1861 ccgcctgatc gaggcctgag agaacgcgtc gatgaacttg tgccccgcta gaggggcggc
     1921 ttctcgtccc gccatcttcc tttgagccag ttgaccttca taagcagcgg aaccatcaag
     1981 ccgtacagaa gaagccccac cgcgtccgcc ccgagatcga acaacgaaca ttcgcgatag
     2041 gggatgaagt actgcgtgat ctcgatggcc aagccgtaag cgagcaagga caggatcttg
     2101 acagcgccga agccgctctc cggccaggag aaatcagcaa gcagggcaag gacggaaaag
     2161 gcaaagacgt gttccacctt atcgtttaca tcctcgacgc ccggtatttc gcggtccgtc
     2221 gtggcgaggt agaatattac gccaaggctt atggccagca ggagccgata ccatcgggtg
     2281 actcttttct gctgcacgat cctaccccct gtatgccttg atctccgcga tgaattttcc
     2341 gccgtagcgc tccagcttga cgtcgcccac ccccgcgata cgccgcatgg agtcggctgt
     2401 cacgggaaag tgccggcaca tctcgtgcag ggtcttgtct gaaaaaatga tatacggcgg
     2461 tacatgattg tcttctgcga tcctcttccg gagcgcgcgc agcctctcga agagcgcccg
     2521 gtcgagctct ccggccccgg cggtctttct tcgccgaccg gcggcaatcc cctcttcgcg
     2581 cctgatcccc gatacccgcc cgcttccggt gagcatcgcg gcgcctgcag
//
LOCUS       DASSLD010000163         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_670985,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000163 DASSLD010000000
VERSION     DASSLD010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670985"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /gene="queF"
                     /locus_tag="VFK23_03020"
     CDS             complement(<1..215)
                     /gene="queF"
                     /locus_tag="VFK23_03020"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HET7318089.1"
                     /translation="MKYGTKAIQQAKLELWPNPSPDRNYVIDISYPEFTCLCPRSGYP
                     DFATIKVTYTPDRKVVELKSLKLYLNS"
     gene            338..1381
                     /locus_tag="VFK23_03025"
     CDS             338..1381
                     /locus_tag="VFK23_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET7318090.1"
                     /translation="MTHSFNCIATGIGSLPIAEPDRAAALSLRYLAEAPIWPQLPQRD
                     FREHMDSQYSEALPGIRLDAAKKRFFFDTSDDLTPELEKFFSRFIEKDYDFFRISAEY
                     APGFYSLLHALEKGLPPGARFVKGHITGPLTAGVSLKDQTGRDIVHHDVLFDAVVKGL
                     AMKAAWQVRELGRFGKPVIIFIDEPAMESLGSAFSAVSSEVVAEKLNEIIGIIHELGG
                     IAGIHCCGNADWPLLFNTDVDIVNFDAFGYMDKVMLYPGDIKKFLGRGGALAWGIVPT
                     AAFTGNETADQLVARLEAGLKRLETDGIARESLLRQCLITPSCGMGSLTPEAAEAILK
                     LVREVSDRMQKNI"
     gene            1558..2388
                     /locus_tag="VFK23_03030"
     CDS             1558..2388
                     /locus_tag="VFK23_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET7318091.1"
                     /translation="MPRYLHFTKFFLFSLALHALVVASALFLAEPAPLAPQYTPVRIV
                     NLPQQEVKQLPPLRQPEPARPVPPPSVPRSVVREKTLPPQAVPMPKKFGDSEEVTLPK
                     TSPVSPPAGMPGGAEQGAESGKTGPETGKTGPAPGKQTGPLPFLTQNDIDQLARKGMP
                     ARQPGDESVTLDTDEFKFISYNRWLKIKIESVLKYPELAAVSGYQGTLFIKFDILKDG
                     SLGGFEVLKGSGYKILDDEAERAIRSSAPFQPLPDDWSMERYSIRAAVIFYMNTGYIR
                     "
BASE COUNT          542 a          726 c          681 g          497 t
ORIGIN      
        1 aagctgttca ggtagagctt caggctcttc agctccacga ccttccggtc cggcgtatag
       61 gttaccttga tcgtcgcaaa gtcggggtat cccgagcgcg ggcagaggca ggtaaattcc
      121 ggataggata tgtcgataac gtaattccgg tccgggctgg gattgggcca gagttcaagt
      181 ttggcctgtt gaatcgcctt cgtgccgtat ttcatagcat tcataatacc ggttttaccg
      241 ttgcctgtaa aggcgaaact tggatttgag ttgttttttc ctatagttga atatataatg
      301 atcattacac tgttatttta cgacgacgag gtccttcatg actcacagct tcaactgcat
      361 cgccaccggc atcggcagtc ttccgatcgc ggagcccgac agggcagcgg ccctgtcgct
      421 ccggtatctc gcggaagcgc cgatctggcc ccagctcccg cagagggatt tccgggaaca
      481 tatggactcc cagtacagcg aggccctgcc cggcatcaga ctcgatgcgg cgaagaagcg
      541 gttctttttc gatacatcag atgaccttac gcctgagctg gaaaaattct tcagccggtt
      601 catagagaag gattacgact ttttcaggat atcggcggaa tacgctcccg ggttttactc
      661 cttgctgcac gcactggaga aaggccttcc cccgggagcg cggttcgtca aaggccacat
      721 caccggcccc ctgactgccg gcgtatcctt aaaagatcag accggcaggg acatcgttca
      781 ccatgacgtt ctcttcgacg ccgtggtcaa aggactcgcc atgaaggcgg cctggcaggt
      841 gcgggaactc ggccggttcg gcaagcccgt catcatcttc atcgacgaac ccgccatgga
      901 atcgctcgga tcggccttct cggcggtctc ttccgaggtc gtggcggaaa aattgaacga
      961 gatcatcggc atcatccacg aactgggcgg catcgccggc atccactgct gcggaaacgc
     1021 ggactggccc ctgctgttca acacggacgt ggacatcgtg aacttcgacg ccttcggata
     1081 catggacaag gtgatgctct accccgggga tatcaaaaaa ttcctgggcc gcggcggggc
     1141 gctcgcatgg ggtatcgtgc ccaccgcagc gttcacgggg aacgagaccg cggaccagct
     1201 cgtggcaaga ctcgaggccg gcctgaaacg cctggaaacc gatggcatcg cacgggagtc
     1261 cctcctccgc caatgcctca ttacgccatc ctgcggcatg ggaagcctca cgccggaggc
     1321 ggcggaggcg atcctgaagc tcgtccggga agtctcggac agaatgcaga aaaacatata
     1381 accacggatg aacacggatt aacacggata aaaccaaaaa cgataaattg gacgcagata
     1441 aaatccgata aacccggata aagacataga ttcagaattg aagaagtgtc ttttgagaaa
     1501 tgatttcatc cgcgtttatc agcgtaaatc tgcgtccaaa gttttttcat aacatacatg
     1561 ccccggtatt tacacttcac gaagtttttc ctcttttccc tcgcactcca cgcgctggtc
     1621 gtcgcatctg ccttgttcct ggccgaaccc gctccgcttg ccccccagta tacgcccgtc
     1681 aggatcgtca acctgccgca gcaggaggtc aaacaacttc ctcccctgcg gcagccggag
     1741 cccgcgcgtc ctgtaccgcc gccgtccgtc ccacgctccg tggtgcggga aaaaaccctc
     1801 cccccgcagg ccgtgcccat gccgaagaaa ttcggcgata gcgaggaggt cacgctgccg
     1861 aagacttcgc ccgtttcgcc gccggcgggc atgcccgggg gggctgaaca gggagcggaa
     1921 tcgggaaaga cgggaccgga gacaggaaag accggtccgg cgccgggaaa acagaccggg
     1981 cctctcccct ttctgaccca gaacgacatc gaccagctcg cgcgcaaagg catgccggcc
     2041 aggcagcccg gtgacgagtc ggtaacgctc gacaccgatg aattcaaatt catatcctat
     2101 aatcggtggc tcaagataaa gatcgaaagc gtgctcaaat accccgagct cgccgccgta
     2161 tcgggttacc agggaacgct ctttatcaag ttcgatatcc tgaaggacgg atcgctcggc
     2221 gggttcgagg tcctgaaggg gtccggatac aagatcctgg acgacgaggc ggaacgggcc
     2281 atccgttcat cggcgccgtt ccagccgctt cccgacgact ggagcatgga gcgctactcg
     2341 atccgggccg ccgttatttt ttacatgaat acggggtata tccggtgacc gacctcgtca
     2401 aaataggcgc cgcattcgcg gtgatcgtcc tcctgctccg cctgcg
//
LOCUS       DASSLD010000164         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_18086,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000164 DASSLD010000000
VERSION     DASSLD010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18086"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..634
                     /locus_tag="VFK23_03035"
     CDS             209..634
                     /locus_tag="VFK23_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HET7318092.1"
                     /translation="MIDINSALFFQLANFLVLLVVLNVALFKPIRQIMQERELDISSA
                     FGDAKTAQERMQHLLESYNASLAEAKQKAASTYNTIYQQGLDAHRDMIAAERAKAGEM
                     LDKARAEIAAASAAAQADLKKEAERLSQEITSKLLGRAV"
     gene            695..1285
                     /locus_tag="VFK23_03040"
     CDS             695..1285
                     /locus_tag="VFK23_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318093.1"
                     /translation="MKSIKKIIDAVFCTAGTWMFAAIAFAEEAGHEGHEEITFMGDWL
                     PRLVNFAIIAAVVVYFMRKPVRDFFKNRSAEIAKAMRESQEARERAAAALAEMERKIK
                     ELEAEMARMVADAKERGEKDRQALVDEGKKVAADVQAQVKQGIDIEVQKAKAALAVEA
                     AQLSVDLAEGKIKKTINNQDHERIVKEYISKVGGKG"
     gene            1282..1818
                     /gene="atpH"
                     /locus_tag="VFK23_03045"
     CDS             1282..1818
                     /gene="atpH"
                     /locus_tag="VFK23_03045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HET7318094.1"
                     /translation="MIRIIAKRYAKALVQLAEENKTVDQTRADLAAFVGAVDTQPAMQ
                     KLFASPVFTPQDKKSVIGELAGKLGMQATTQRFVEHLAETGRIRYVRDVHEAFLEILA
                     LRQNRAMARLTTAASINNGDLANIKKTLEALTGKQVDIDSKVDASLIGGAKAQIGSTI
                     YDGTIKNQLSKMRMKLVN"
     gene            1930..>2103
                     /locus_tag="VFK23_03050"
     CDS             1930..>2103
                     /locus_tag="VFK23_03050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940787.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET7318095.1"
                     /translation="MQIKAQEISEIIKKKISDFEKTVEVAEVGTVITVGDGIAKVFGL
                     DNVMSGELLELPGG"
BASE COUNT          526 a          580 c          615 g          382 t
ORIGIN      
        1 gcttttcggt ccggcacggt gctgcgaagg acctcttgtc cgcgttcgtt ccatcgtccc
       61 cgttccctcc tgattctata atcaaaatcc ctacgctcat ttatattatt tataggcaaa
      121 attcttgaca atgttttttc gttgtgttaa ctttttcccg tttcaaaggg tcaaacgagc
      181 gtttttcttt cccaggggcc atcgcgctat gatcgatata aacagtgcac tcttcttcca
      241 acttgccaac tttctcgtgc tgctcgtcgt attgaacgtt gctcttttca agcccatcag
      301 gcaaatcatg caggagcgcg aactggacat cagttcggcg ttcggagacg ccaagaccgc
      361 gcaggagcgc atgcagcacc tcctcgaaag ttataacgcc tccctggccg aggccaagca
      421 gaaggcggct tcgacgtaca acacgattta ccagcagggc ctcgatgccc atcgcgacat
      481 gatcgccgcc gagcgcgcca aagcgggcga gatgctcgac aaggcgcggg ccgagatcgc
      541 cgccgcgtcc gccgcggcgc aggccgacct caaaaaagag gcggagaggc tctcgcagga
      601 aatcacctcc aaactgctcg ggagggcggt ttaaaaacgg tagccgcagg cttcagcctg
      661 cgcgagatca gagttcagag tataaaacca aaccatgaaa agcataaaaa agatcataga
      721 tgcggtattc tgcacggcag gaacgtggat gttcgcggcg atcgcctttg cagaagaagc
      781 ggggcacgag gggcatgaag aaataacctt catgggagac tggctgccga ggctcgtcaa
      841 tttcgcgatc atcgccgcgg tcgtcgtcta tttcatgcgg aaaccggtcc gggatttttt
      901 caaaaaccgt tccgccgaaa tcgcgaaggc gatgcgggaa tcacaggaag cccgggagcg
      961 cgccgcggcg gccctcgccg agatggagcg caagatcaag gagctcgagg ctgaaatggc
     1021 acggatggtc gccgacgcga aggagcgggg cgagaaggac cggcaggcgc tcgtcgacga
     1081 gggcaaaaag gtcgccgccg acgtgcaggc ccaggtcaaa cagggcatcg atatcgaggt
     1141 tcagaaggcg aaggccgcgc tcgcggtgga ggcggcgcag ctctcagtcg atcttgcgga
     1201 aggcaagatc aagaaaacga tcaacaatca ggaccatgag cggatcgtga aggaatacat
     1261 ctccaaagtg ggaggcaagg gttgatccgg atcatcgcaa agaggtatgc caaggccctc
     1321 gttcagctcg ccgaggaaaa taagaccgtt gaccagacaa gggccgatct ggccgctttt
     1381 gtcggcgccg tggacaccca gccggccatg cagaagcttt ttgccagccc cgtattcaca
     1441 ccccaggaca aaaaatcggt catcggggaa cttgccggga agctgggcat gcaggcgacg
     1501 acccagcgct tcgtggaaca cctggccgaa acaggcagga tccggtacgt cagggacgtg
     1561 catgaggcgt tcctggagat ccttgcactt cgccagaatc gtgcgatggc gcgactcacc
     1621 accgcggcgt cgataaacaa tggagacctt gcaaatatca agaagacgct cgaagctctg
     1681 accggcaagc aggtggacat cgactcgaag gtcgatgcat ccctgatcgg gggcgcgaag
     1741 gcccagatcg ggagcactat ttacgacggg acgatcaaga accagctcag caagatgcgt
     1801 atgaagctgg tgaactgaat aaacttcagg agacagaaga caggagccag aagccagaag
     1861 aaaagcagaa cgcttttctc ctgactcctg cctcctggct cctgactcct ctctctacga
     1921 ggtgtaacaa tgcagatcaa ggcacaggaa atcagcgaga tcatcaaaaa gaagatctcc
     1981 gacttcgaga agaccgtgga ggtcgccgaa gtgggcaccg tgatcaccgt cggagacggc
     2041 atcgccaagg tcttcggact cgacaacgtc atgtccggcg agcttttgga actccccggc
     2101 ggc
//
LOCUS       DASSLD010000165         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_605328,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000165 DASSLD010000000
VERSION     DASSLD010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605328"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFK23_03055"
     CDS             complement(<1..430)
                     /locus_tag="VFK23_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7318096.1"
                     /translation="MAKSILIVEDEETLRESIKRIFTKEGYAVDGAESAEKGLILLET
                     NHYDVIISDIILPGMDGIEMLSRVREQNPDQIVIIITAYASLDTSVKALRAGAYDYIM
                     KPIIHEEIKQIVRNALKQKSLQTENTLLKRQIDREYDFSSI"
     gene            complement(489..>2135)
                     /locus_tag="VFK23_03060"
     CDS             complement(489..>2135)
                     /locus_tag="VFK23_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7318097.1"
                     /translation="GAMAGIIAGFSVWIYTLLIPALMRAGIIEAGGVMEGVFNSTLLK
                     PTALFGLEGLDRWSHSLFWGLFLNIGFYVGVSIFTRQSDAETHQAIVFVDSFSPLPLT
                     PAHRPANIAEVEDVLGQYIGPTEAREAVDAFLKKHRLNRESISGEWLLKLRQEAERIL
                     SGSLGTSISTLVFQDRTVLTHDEAMRVSDSIQQISRSLRLSRQELAEANRQLAMLKEF
                     SENIIESLPLGVATLDEGLSVKYWNSGMEMITGVEKSDALNTDASDLLTCLEPRLFTP
                     QIHEGEITCRRNFDPQIVLKGYVSRLTGAQKGYVLVLEDITEKKKIEEELFRATKHAS
                     VGRLAAGVAHEIGNPLASISSLVQELQTEELTAFTKESLTTINQHVHRIARIVRSLGD
                     FARLYPRQKVPTSLREILENTLNLVRYDKNFRKIEIHTDVRDTPALKIDPDQIQQVFL
                     NLILNARDAMPDGGKLDISIHQSDGNVEMQFSDTGHGIDPDVRDKVFDPFFSTKGPTK
                     GTGLGLSICYSIIKDHGGTIEIDSEKDKGTRFTIKIPVEG"
BASE COUNT          365 a          639 c          650 g          481 t
ORIGIN      
        1 tgatgctgct gaaatcatac tccctgtcga tctggcgttt gagcagtgtg ttctcggtct
       61 ggaggctctt ctgcttgagc gcgttcctga cgatctgctt gatctcctca tggatgatcg
      121 gcttcatgat ataatcgtaa gccccggccc ggagcgcctt caccgacgtg tcgagcgacg
      181 cgtaagccgt gatgatgatg acgatctggt ccgggttctg ctcccgcacc ctggaaagca
      241 tctcgatccc gtccatgccc ggcaggataa tgtccgagat gatcacgtcg tagtggttcg
      301 tttcgagcag gataagcccc ttctccgccg attccgcgcc gtcaacggcg tacccctcct
      361 tcgtgaagat ccgcttgatg gactcccgca gggtctcctc gtcttccaca atgagaatac
      421 tttttgccat tgaaacctcg ttttttcagc ctgccggctt gccagctcgc aagccgacgg
      481 aaattaatct acccttccac ggggatttta atcgtgaacc tcgtgccctt gtccttctcc
      541 gaatcgatct cgatcgtgcc gccgtggtcc ttgatgatgc tgtagcagat ggacaggccc
      601 agtcccgtgc ccttggtcgg ccccttggtg ctgaagaacg gatcgaagac cttgtccctc
      661 acgtcgggat cgattccgtg gcccgtgtcc gaaaactgca tctcgacgtt gccgtcgctc
      721 tggtggatcg atatgtcgag ctttccgccg tcgggcatgg cgtcccgcgc gttcaggatg
      781 aggttcagga agacctgctg gatctggtcc gggtcgatct tgagcgcggg cgtgtcccgc
      841 acgtcggtgt ggatctcgat cttccggaaa ttcttgtcgt accgcaccag gttcagcgtg
      901 ttctccagga tctccctgag gctcgtcggg accttctgac ggggatagag ccgggcgaaa
      961 tcgccaagac tccgcacgat cctcgcgata cggtgcacgt gctggttgat cgtggtgagc
     1021 gattccttcg tgaacgccgt gagttcctcg gtctgcagtt cctgcacgag cgaggagatc
     1081 gacgcgagcg ggttcccgat ctcgtgagcg acgcctgcgg cgagtctgcc gacgctcgcg
     1141 tgcttggtgg cacggaagag ttcctcctcg atcttcttct tctccgtgat gtcctcgagc
     1201 acgaggacgt accccttttg cgcgcccgtg agccggctca cgtacccttt caggacaatc
     1261 tgggggtcga aatttctcct gcaggttatt tctccctcgt ggatctgcgg ggtgaaaaga
     1321 cggggctcga ggcacgtgag caggtcggag gcgtccgtgt tgagggcgtc ggacttctcc
     1381 acgcccgtga tcatctccat gccgctgttc cagtacttga cggagagccc ctcgtccagg
     1441 gtcgccacgc ccaggggaag gctttcaatg atgttttcgc tgaattcctt cagcatggcc
     1501 agttgacggt ttgcctcggc gagttcctgc cgggacagcc tgaggctgcg cgagatctgc
     1561 tggatcgaat cggaaacgcg catcgcttcg tcgtgggtca ggaccgtccg gtcctgaaac
     1621 accagcgtgc tgatcgaggt gccgagcgaa cccgacagga tgcgctcggc ctcctgccgg
     1681 agtttcagca gccattctcc gcttatcgat tcccggttga ggcggtgctt cttgaggaag
     1741 gcgtcgaccg cttcgcgcgc ctcggtgggg ccgatatact ggccgaggac gtcctcgacc
     1801 tcggcgatat ttgccggacg gtgcgcgggc gtcaggggaa gcggcgaaaa tgaatcaaca
     1861 aaaacgatgg cctggtgcgt ctccgcgtcg gattggcggg tgaaaatcga gacgcccacg
     1921 tagaaaccga tgttcaggaa caggccccag aacagggagt ggctccaccg gtccagccct
     1981 tccaggccga agagcgccgt gggcttgagc agcgtggagt tgaagacccc ctccatgacg
     2041 ccgcccgcct cgatgatccc tgcgcgcatg agggcgggga tcaagagcgt gtagatccag
     2101 acgctgaaac ccgcgatgat gccggccatg gcgcc
//
LOCUS       DASSLD010000166         3832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_811430,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000166 DASSLD010000000
VERSION     DASSLD010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811430"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..478
                     /locus_tag="VFK23_03065"
     CDS             38..478
                     /locus_tag="VFK23_03065"
                     /inference="COORDINATES: protein motif:HMM:NF019473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7318098.1"
                     /translation="MTLQEKNIKTVKDLFETCKKGDYDAAMNYVTEDCDWWSPVTNAK
                     SEFISWATPRHGRAEVSSLFKEIYSKIKPIEVKPEHFTAQNDSVIVEGSSRGMANATG
                     CEFFINWVMVCTLSDGKIKRFRKYYDTAELSNAFLARCEVRKAA"
     gene            complement(569..1828)
                     /locus_tag="VFK23_03070"
     CDS             complement(569..1828)
                     /locus_tag="VFK23_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318099.1"
                     /translation="MKIVKAALLISTALIVSTSLLVGCGRGGKDNSSSNKTSNSTGIS
                     LSGTAATGAPIASATITLKDKNGSLKTALTGTDGKYYFDITGMAAPFLLKVPLGQSSL
                     YSVAASSGTVNVHPFTDLIIRNWYAGQGSDVDAAFGSAGNLPSPPTADDIATRETAVR
                     NILSTWLQQEGVDASSFNLITSSFDANETGFDEVLEYTEVEIDTAGRVTVSCTDPATG
                     IGNTMVSANIGNLTAADTTPPSDPVSLRAIGADRNKILLLWDASTDNVGIAGYNVYRG
                     NTRIGKAAYSVYFDSAGLAPGTAYCYQIEAFDGAGNTSASRSGQVCGSTLAVSDTTPP
                     SAPADLAVSQVSSTQISLAWTASTDDVFVLSYRVLRDGGELAKVPSTSFVDGDLAAHT
                     RYCYTVKAVDAAGNISADSSQICARTP"
     gene            2058..2423
                     /locus_tag="VFK23_03075"
     CDS             2058..2423
                     /locus_tag="VFK23_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HET7318100.1"
                     /translation="MKTKKNLIIVGDRVLIDPDEQMDKTSSGLYLPPTVKEKEKVLGG
                     HVVKTGPGYAIPDTTPGESWQSGNKEVKYVPLQAVEGDYAIFLKESSMEIEFEEKKYL
                     IVPHSAILALVRTELSEDN"
     gene            2685..>3832
                     /locus_tag="VFK23_03080"
     CDS             2685..>3832
                     /locus_tag="VFK23_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET7318101.1"
                     /translation="MKKNLTYKILESHLVEGELVPGREIGIRIDHTLLQDATGTLAML
                     EFEALGIPGVKAELAAQYVDHNLLQTDNKNADDHKFLQTAAARYRVHFSRPGNGVSHQ
                     VHMERFGVPGKTLLGADSHTPGAAGVSMLGIGAGGLDVALAMAGYPYYFPCPKILGVR
                     LTGSLPDWVSAKDVILEMLRRYDVKGCVGKIIEYYGPGVRTLSATDRETIGNMGTETG
                     ATTSIFPSDERTREYLIAQGRGDAWVPLSADDSAEYDEYVEIDLSKVEPLIARPSSPG
                     NVVPVREIAGIKVDQAIVGSSVNSSFRDLMVSARMLEGRRVHTNTTFHVNPGSRQVLE
                     NVYQAGGLLAFLTSGARLHEPGCQGCIGMGQAPGTGEVSLRTFPRNFPG"
BASE COUNT          892 a         1048 c         1097 g          795 t
ORIGIN      
        1 ttccgtgatg aaatataaaa aagaaaggag ttttgccatg acattacagg aaaaaaatat
       61 aaaaacggta aaagatttat ttgaaacgtg taagaaggga gattacgatg ccgccatgaa
      121 ctatgtgaca gaagactgcg actggtggtc tccggtaaca aatgccaaga gcgaattcat
      181 atcatgggcc accccccggc acggtcgcgc ggaagtctcc agcctgttca aggaaatata
      241 ttcaaagatc aaacctatcg aagtcaagcc ggaacatttt accgcccaga atgactccgt
      301 tatcgtcgaa ggatcatcgc gcggcatggc gaacgccacg ggatgtgagt ttttcatcaa
      361 ctgggtaatg gtgtgcacgc tgtcggacgg caagatcaaa cggttccgca aatactatga
      421 cacggctgaa ctatccaatg cgtttctggc aagatgcgaa gtacgcaagg cggcgtgaag
      481 tcgtaaagca agccgcaggc cgttgtcgct caaaagggga tattggttca aagagaatcg
      541 atatcccctt tggcgtttga ggatgggtct atggagtcct tgcacagatc tggctgctgt
      601 ccgcagaaat gtttcccgct gcgtcgacgg ccttgacggt atagcaatac cgagtatgcg
      661 cggcgagatc tccatcgacg aagctcgtcg aagggacttt tgcgagctct ccgccgtccc
      721 gcaatactct gtagctcaga acgaaaacgt catcggtaga agccgtccat gcaaggctaa
      781 tctgcgtcga ggagacctgc gatacggcaa gatcggcggg ggccgagggc ggcgttgtat
      841 cggaaactgc gagggtcgag ccgcatacct gaccgctcct ggaggcggat gtatttcccg
      901 ccccgtcgaa ggcctcgatt tgatagcagt acgcggtgcc gggcgcaaga cccgctgaat
      961 cgaaatagac tgagtaggcg gccttgccga tcctcgtatt tccccgataa acgttgtagc
     1021 ccgcgatgcc gacattgtcc gtcgaggcat cccagagcag gagaatcttg ttcctgtccg
     1081 cgcctattgc cctgagactg acggggtcgc tcggtggggt cgtatcggct gccgtcaggt
     1141 tgccgatgtt cgcggagacc atagtattac ctattccggt cgccggatcg gtgcacgaaa
     1201 cagtcacccg gcccgcggta tcgatctcga cctccgtata ttccaggacc tcgtcgaacc
     1261 cggtttcgtt cgcgtcaaag gacgatgtga tcaggttgaa ggacgatgca tcaacccctt
     1321 cctgctggag ccaggtcgac aggatgttcc tcactgcggt ctctcttgtt gctatatcgt
     1381 cggcagtagg cggcgacgga aggtttcccg cacttccgaa cgctgcatcc acatcactcc
     1441 cctggccggc gtaccagttc cttatgatga ggtcggtaaa agggtgaacg ttgaccgttc
     1501 cggaagaggc tgctacgctg taaagagagg actggcccag ggggactttg agaagaaacg
     1561 gagcggccat gcctgtaatg tcgaaataat atttaccgtc cgtacccgtg agggccgtct
     1621 tcagggagcc gttcttgtcc ttcagtgtta tcgtcgccga cgcaatgggc gcacccgttg
     1681 cggcagtgcc cgacagcgag atgccggtgc tgttgctggt tttattggac gatgaattat
     1741 ctttgccccc gcgtccgcag cccacgagca aagaggtcga gactatcagc gccgtggaaa
     1801 taagaagcgc cgcctttact atcttcacgt tatgccctcc tttagctacg atccttaata
     1861 caacatataa taacattaag caatgtctat gcctaagagg aagcaattga tattccttta
     1921 aataacaaat ggttataatg ttcaatcagg gctcgatggc tgaatttttt cagtttgggc
     1981 cagccaattt gaacaagaag gtcaattctc gattcactgc atgacaagtc aaaatacttt
     2041 cgtgtatact ctgttccatg aaaaccaaaa agaacctcat catcgtcggc gacagggtgc
     2101 tcatcgaccc tgacgagcag atggacaaga cctcatcggg cctttatctt cccccgaccg
     2161 ttaaggaaaa agagaaggtc ctgggaggcc atgtcgtaaa gacggggcct ggatatgcga
     2221 tcccggatac gacgcccgga gagtcctggc aatcgggcaa taaagaggtg aagtacgtcc
     2281 cgctccaggc ggtggaaggc gactatgcca tcttcctcaa ggaatcgtcg atggagatcg
     2341 agttcgaaga aaaaaagtac ctcatcgtcc ctcattcggc catccttgcg ctcgtaagga
     2401 cggagctatc ggaagataat tagcactgcc gtcatgtcct cctcggggta tcgctgctct
     2461 ttcatcgtcc ccaccccgcg cgtccacgcg gcgcgaccga ccgttcccgc agaaaacgcg
     2521 attttccccg gttctgcagc ggccgggagc cgtcctccag ccgataaatt gtggtaaaat
     2581 gaattaggaa aatcgaatga ggtgtctcct cgataaaaaa gataaaatca gtccagattt
     2641 gaggcggttg cggtctccgc ggcgggcggg agacggagaa gaccatgaaa aaaaacctga
     2701 cttataaaat tttggaatcc catttggtag aaggcgaact cgttccaggc agggagatcg
     2761 gcatccggat cgatcatacg ctcctgcagg acgcgacggg aacgctggcg atgctcgagt
     2821 tcgaggcgct gggcataccc ggagtgaaag ccgaactcgc ggcgcagtac gtggaccaca
     2881 accttcttca gaccgataat aagaacgccg acgaccacaa gtttctccag acggccgcag
     2941 ccaggtaccg cgtccatttc agcaggccgg gcaacggagt ttcacaccag gtgcatatgg
     3001 aacgcttcgg cgttccgggc aagacgcttt tgggggcgga cagccataca cccggcgctg
     3061 ccggggtatc catgctcggc atcggcgcgg ggggccttga cgtggccctg gccatggcag
     3121 gctatccgta ttatttcccc tgcccgaaga tactcggcgt ccggctcacg ggcagtcttc
     3181 ccgactgggt gagcgccaag gacgtgatcc tcgaaatgct gcggcgctac gacgtcaagg
     3241 ggtgcgtggg caagattatc gagtattacg gaccgggcgt ccgaaccttg tccgcgacgg
     3301 accgggagac catcggcaac atgggcaccg agaccggggc cacgacctcc atctttccct
     3361 ccgacgagcg cacccgggaa tacctgatcg cccagggccg gggagacgca tgggttccgc
     3421 tttctgccga cgacagcgcg gagtacgatg aatacgtcga gatcgacctt tccaaggtgg
     3481 aacccctgat cgccaggccg tcgtcgcccg gaaacgtggt gccggtgcgg gaaatcgccg
     3541 gcatcaaagt ggaccaggcc attgtgggaa gcagcgtgaa ctcctccttc cgcgacctca
     3601 tggtgtcagc ccgaatgctc gaaggcaggc gcgttcatac caacacgacc ttccatgtga
     3661 atcccggaag caggcaggtt ctcgaaaacg tgtatcaggc cggcgggctc ctcgcctttc
     3721 tgacttccgg cgcgcgtttg catgaacccg gctgccaggg gtgcatcggc atggggcagg
     3781 cgccgggcac cggcgaggtg agtctccgca ccttcccgcg aaacttcccg gg
//
LOCUS       DASSLD010000167         4775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1297192, whole genome shotgun sequence.
ACCESSION   DASSLD010000167 DASSLD010000000
VERSION     DASSLD010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297192"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="VFK23_03085"
     CDS             <1..1232
                     /locus_tag="VFK23_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HET7318102.1"
                     /translation="LPIRVITELAWHSLFARNMFVQATPEELERHIPRFTVIDLPSFH
                     AIPSVDGTNSETFIIISFAKKAVIIGGTSYAGEIKKSVFTVLNYLLPLEKNIFSMHCS
                     VNVGLKGDSAVFFGLSGTGKTTLSADPNRSLVGDDEHGWDERGVFNFEGGCYAKIINL
                     SKEAEPEIYETTRRFGTILENVAIDSRTRRIDLTDNSFTENTRASYPLTHIPNIVATG
                     STVHPSNIIMLTCDAFGVLPPIARLTKEQAMYHFLSGYTARVAGTEAGVKEPSATFST
                     CFGGPFLALNPTVYGELLREKIARHNVTCWLVNTGWSGGPYGVGSRMKIAYSRALINA
                     ALDGTLNVGSFEKDPVFGLDIPSSCAGVPAEVLNPRNTWFDKAKYDETAAKLVGMFKD
                     NFAKYAPHVLSEVANVL"
     gene            1285..3156
                     /locus_tag="VFK23_03090"
     CDS             1285..3156
                     /locus_tag="VFK23_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HET7318103.1"
                     /translation="MYQRVLLVDPATGFYKMKRYGFDRYFGPVDLGIHLSETYRSLNF
                     GVGVFAGSIFPGSNRLVVTGVSPCWQGYYISSMGGAGLVFNNLGINMLSLVGKAPVPS
                     VLYLNRNHGEEIEVEVVPIDVETVWKEGRTGVYSLTDRVYRMFGSRYENDLRILAVGP
                     AALQTDMGGIMSVPITGGKISHIDTWAGRGGFGSAMAREHGIVAVIYGGTAADEDFRD
                     RTVADEWFRNKYDQRLMQKDLEVTTKYRYDENLHTGGTFGVNFATTGGRIMAFNYRTI
                     HLTEEERTSFHQKFIVDHYLKQFNEETIVKRQQGTCGEPCAAVCKKMNGIYKKDYEPY
                     QTMGPLCGVFDQRAAEKLNHHCDAMGFDAISCGGVLAWLMDLLDEGLLTPEELGVTRM
                     PRWDAAGFDIVEDSMHNAELGCELIDAILERSGILDFREGVRKWSRIRSREKGTALHD
                     RLVYIAFSRRGWMVPNQYWVSGVLVPMAIMGKYYMIYSFDFISPRTLGRMCAERMKKE
                     LLLDNLGICRFHRGWAEELLPEVMGNLYDCRERFLRAIDVLASRLHSRNSPVFWESER
                     NIDFLHTFLKRKKEIDRDGHPELSAWLDKFCRDRVEAAREFWYETLKGIDESLREFF"
     gene            complement(3231..3803)
                     /locus_tag="VFK23_03095"
     CDS             complement(3231..3803)
                     /locus_tag="VFK23_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HET7318104.1"
                     /translation="MHVLFLAPVFVWAGCASPRSNFYSLGSTAKPVAASAGYSVAVGP
                     VTVPAEVDRPQMVVRVGPNEVVVEEFHRWASSLPDAIARAVAGNLSAALGTPYVSVYP
                     EPTSAGARYRVVIDVMGFDSAPGESASLDAVWAVRSARDGTSRAGRTLAREAPQEKGY
                     PALAAAHSRALGKMSSDIAEAIRALERGRP"
     gene            complement(3818..>4775)
                     /locus_tag="VFK23_03100"
     CDS             complement(3818..>4775)
                     /locus_tag="VFK23_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET7318105.1"
                     /translation="EGISVRTESVTALLVGGVAFETLPSGEVAESAPSTVFPLFRDRK
                     AALSPANKGVQRYVLRTRQSLRGLSVGAPVQFLGMTIGEVVHFGLEYNRESQDLRPRV
                     EIETYEHLIEEQLGKKAAEAIRKSTTPEGRHAIIQTMVVEKGLRAQLRPTSLLGGQLF
                     VALDYFPDAPKARIDWKQNPPEFPVVPGGAEELEEKVRSIMAKLDRVPVEEIGDDVRK
                     AVGSLDQTLQSATRTLDRVDGETLPEAKKTLEDLRRAIAAAERLLANTDNAFLGPDAA
                     AQQELRDALREIARSARAIRMLADYLEQHPETLIRGKNKENP"
BASE COUNT          934 a         1491 c         1440 g          910 t
ORIGIN      
        1 gcctcccgat tcgcgtgatc acggaactgg cgtggcactc cttgtttgcc cgcaacatgt
       61 tcgtgcaggc gacgccggag gaactcgaga gacatatccc tcggttcacc gtcatcgacc
      121 tgcccagctt ccacgccata ccttcggtgg acggcaccaa ctcggaaacc tttatcatca
      181 taagcttcgc aaaaaaagcg gtcataatcg gcggcaccag ttatgcggga gagatcaaga
      241 aatcagtgtt cacggtactt aactacctct tgccgctgga gaagaatatc ttttccatgc
      301 actgttccgt aaacgtcggt ctgaaggggg attcggcggt gttcttcggg ctctccggta
      361 ccggcaagac caccctctcc gccgatccga accgttcact ggtaggtgac gacgaacatg
      421 gttgggacga aaggggggtc ttcaattttg aaggggggtg ctacgccaag atcattaacc
      481 tgtccaagga agccgaaccg gagatctatg aaacaacccg cagattcggc accatcctgg
      541 aaaacgttgc cattgacagc cgtacccgcc gcatcgacct gaccgacaat tctttcaccg
      601 aaaacacccg ggcctcatac ccgctgactc acattcccaa catcgtggct acgggcagta
      661 ccgttcatcc gagcaatatc atcatgctga cctgcgatgc cttcggcgtg cttcccccga
      721 tcgcccgtct gaccaaggaa caggccatgt accacttcct gtccggctat accgccaggg
      781 tggccgggac cgaagccggg gtaaaggagc catcggcaac attctcaacc tgtttcggag
      841 gccccttctt ggcgctcaat ccgacggtgt acggcgagtt gctacgcgaa aagatcgcca
      901 gacacaacgt cacctgctgg ctggtcaaca cgggctggag cggcggaccc tacggagtgg
      961 gctcccgcat gaagatcgct tattcacgcg ctctgatcaa cgccgcactg gacggcaccc
     1021 tgaatgtcgg ctcgttcgag aaggatccgg tcttcggtct ggacatccct tcttcgtgtg
     1081 ccggcgtacc tgccgaggtg ctgaatccgc gcaacacctg gttcgacaag gcaaagtacg
     1141 atgagactgc cgcaaagctg gtcgggatgt tcaaggataa tttcgccaag tacgcgccgc
     1201 acgtattgtc agaggttgca aatgtcctgt gacaaccccc ccaacaaaga gagaggtctt
     1261 gcgcgctttc ggaggtaaat agcaatgtat cagagggtgt tgctggtcga tccggccacc
     1321 ggtttttaca agatgaaaag gtacggcttc gaccgctatt ttggtccggt cgacttgggt
     1381 attcaccttt ccgagacata ccggagcctc aatttcggtg tcggcgtttt tgccggctcg
     1441 atctttccgg gctcgaaccg cctggtggta accggggtct ccccttgctg gcaagggtat
     1501 tacatcagtt ccatgggtgg cgcggggctg gtcttcaata acctggggat caacatgctc
     1561 agcctggtgg gaaaagcccc ggtcccgtcg gttctctacc tgaaccgtaa ccacggagag
     1621 gaaatcgaag tggaggtggt tcccatcgat gtcgaaactg tctggaaaga gggacggacc
     1681 ggggtctact ccctcacgga ccgggtctac cggatgttcg gcagccgcta cgagaacgat
     1741 ctccgcatcc tcgcagtcgg cccggcagcc ctccagaccg acatgggggg aatcatgtcg
     1801 gttcccatca ccggggggaa gatcagtcac atcgatacat gggccggccg cggcggtttc
     1861 ggcagcgcca tggcccggga gcacggcata gtggctgtca tctatggcgg cacggcggcc
     1921 gacgaggatt tccgggaccg tacggtggcc gacgagtggt tcaggaacaa atacgaccag
     1981 cggctcatgc agaaggatct ggaggtgacc accaagtacc gctacgacga aaatctccat
     2041 accggcggca cgttcggcgt gaacttcgcc acaacgggcg gccggatcat ggcgttcaac
     2101 tatcgcacta tccatctgac cgaggaagag cgcacctcct ttcatcagaa atttatcgtc
     2161 gatcactacc tcaaacaatt caatgaagaa acgatcgtca aacgtcagca aggcacctgc
     2221 ggcgaacctt gcgccgcagt ctgcaagaag atgaacggta tctacaagaa ggactacgag
     2281 ccgtaccaaa ccatgggacc cctctgcggc gtattcgacc agcgtgccgc cgagaagctc
     2341 aaccatcact gcgacgccat gggcttcgat gccatctcct gcggaggtgt gctggcctgg
     2401 ctcatggacc ttctggacga agggctcctg accccggaag aactcggcgt cacccggatg
     2461 ccccgctggg atgcggcggg cttcgacatt gtcgaagact cgatgcacaa tgccgaactg
     2521 gggtgcgagt tgatcgacgc cattctggaa cggagcggca tcctggattt ccgggagggc
     2581 gtgcgtaaat ggagccgtat ccgttcccgc gagaaaggta cggcgcttca cgaccggttg
     2641 gtctacatcg ccttcagtcg acggggatgg atggtcccga accagtactg ggtttccggc
     2701 gtcctggtcc ccatggccat catgggcaag tactacatga tttacagctt cgacttcata
     2761 tcaccccgga ctctggggag gatgtgcgcg gagcgcatga aaaaagagct cctcctcgac
     2821 aacctgggaa tttgccgttt ccacaggggg tgggccgaag aactcctccc cgaggtcatg
     2881 ggtaacctgt acgactgccg ggagcggttc ctgcgcgcca ttgacgtgct ggccagccgc
     2941 ctccacagcc gcaacagccc cgttttctgg gaatcggagc ggaacatcga ctttctgcat
     3001 accttcctga aacggaagaa ggagattgat cgggacggcc accccgagct ttccgcatgg
     3061 ctcgataagt tttgtcggga ccgggtcgag gctgcacggg agttctggta cgagaccctc
     3121 aagggaatcg acgaaagcct gcgggaattc ttctgactca aacatttctt catctcgagc
     3181 accgccagac aactcaaagc ctacgggctg gtttgaccgg gcgcggcggc ttacggcctg
     3241 ccgcgctcca gcgcccggat cgcctccgca atatccgagc tcatcttccc gagggcgcga
     3301 ctgtgcgcgg cggcgagggc ggggtagccc ttctcctgcg gcgcttcgcg cgccaaggtg
     3361 cgtcccgccc gcgacgttcc gtcccttgcg gagcggaccg cccagacggc gtccagactc
     3421 gctgattccc cgggcgctga atcgaagccc atcacgtcga tgacgacgcg gtaccgcgcg
     3481 cccgcggagg tcggctcggg gtacacggac acgtacggcg ttccgagcgc cgcggagagg
     3541 ttcccggcga cggccctcgc gatggcatcc ggcaacgaag acgcccaacg gtggaactcc
     3601 tcgacgacga cctcgttcgg gccgacgcgc acaaccatct gcggccggtc gacctcggcg
     3661 ggtacggtga cggggccgac ggcgacggaa tagcccgctg acgcggcgac gggcttcgcg
     3721 gtggaaccca gcgaatagaa gttcgaccgc ggcgaggcgc accctgccca gacgaatacc
     3781 ggcgcgagga aaagtacgtg catggcgagc cggcgcatca gggattctcc ttgttcttgc
     3841 cgcggatgag cgtttcggga tgctgctcga ggtaatcggc cagcatgcgg atcgcacggg
     3901 cggagcgggc gatttccctc aaggcgtcgc gcagctcctg ctgcgcggcc gcgtcgggcc
     3961 cgaggaacgc gttgtcggtg ttcgccagca ggcgctccgc tgcggctatc gcccggcgga
     4021 ggtcctccag cgtctttttc gcctcgggga gggtctcccc gtcgacccgg tccaacgtcc
     4081 gggtcgcgct ctgaagcgtc tggtcgagcg acccgaccgc ctttcttaca tcgtcgccga
     4141 tctcttccac cggcaccctg tcgagcttgg ccatgatgct ccggaccttt tcctcgagct
     4201 cctccgctcc gcccggcacc acggggaatt ccggcgggtt ctgcttccag tcgatcctgg
     4261 ctttcggcgc gtcggggaag tagtcgaggg cgacgaacaa ctgcccgccg aggaggctgg
     4321 tgggtcgcag ctgggcgcgc aacccctttt cgacgaccat cgtctggatg atggcgtgtc
     4381 ttccctcggg ggtcgtcgac ttcctgatgg cctccgctgc cttcttcccc agttgctcct
     4441 cgatcaggtg ctcgtaggtc tcgatctcca cgcggggccg aagatcctgc gattcccggt
     4501 tgtactccag accgaaatgg acgacctccc cgatcgtcat cccgagaaac tggaccggcg
     4561 ccccgaccga cagcccgcgc aaggactgcc gggtacggag gacgtaccgc tggactccct
     4621 tgttggcagg ggaaagcgcc gccttccggt cccggaacag cgggaagacg gtgctcggcg
     4681 cgctctccgc aacctctccg gagggaaggg tttcgaaagc aacccccccg accaggaggg
     4741 ccgtcacgga ctcggtccgg acggaaatcc cctcc
//
LOCUS       DASSLD010000168         2832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_480618,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000168 DASSLD010000000
VERSION     DASSLD010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480618"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFK23_03105"
     CDS             <1..460
                     /locus_tag="VFK23_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7318106.1"
                     /translation="NPKDADAWYHLADVYERTASYREAADALNKVVALDPGRGSAYLK
                     LGTVYNRLGRHQEAVTNFQKATKFFPKDPVLHNNLAVSYGKLGKLNEEIKLLEKAISL
                     RPHYATARYNLAMAFLKQGKRDQALKQYAELEKFDAGTASALKKEIEKNR"
     gene            469..873
                     /locus_tag="VFK23_03110"
     CDS             469..873
                     /locus_tag="VFK23_03110"
                     /inference="COORDINATES: protein motif:HMM:NF017394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HET7318107.1"
                     /translation="MRYSRTIIAQALFSAVVMISAALAADVPCFQCGMIVDFGSKFTA
                     KIAAADRTLLFCDIGDLFAYLKRKKLSADGAFVKDYPSSEWVEAKAAYFVHDEKKFKT
                     PMGWGVAAFRDRGRAAEAGKVMDWDAAARAMQ"
     gene            870..1274
                     /locus_tag="VFK23_03115"
     CDS             870..1274
                     /locus_tag="VFK23_03115"
                     /inference="COORDINATES: protein motif:HMM:NF025745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4418 family protein"
                     /protein_id="HET7318108.1"
                     /translation="MKKLVALTALASGVLLVLVPRFILPACEYEGYPAMHCSDTARAE
                     YLIGALLVLVGGAAFLAKTAKLSLTGAAAALILYAAAYLLPDSIGYCRSPRMPCNYGM
                     VPAVRFIAAVGAATMIIACAGVVNSVRMKGKA"
     gene            1271..1933
                     /locus_tag="VFK23_03120"
     CDS             1271..1933
                     /locus_tag="VFK23_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018939302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7318109.1"
                     /translation="MIELKGLSKSYTISGKTIKAVQHVDLSIAQGEFVSIVGHSGSGK
                     STFLSLVGGIARPDDGTVSIDGTDIWKYSDVQLSKLRNDKFGFVYQFASLIPTLNAAE
                     NVLLPTVFGGKKTRADAVALLERVGLGDKTDRYPSELSGGEQQRVAIARAFINDPMIV
                     LADEPTGDLDEETEAEIMAFFEKINRERKVTMIMVTHSSELALRAPSRLRMKHGVLEK
                     LT"
     gene            complement(2043..2744)
                     /locus_tag="VFK23_03125"
     CDS             complement(2043..2744)
                     /locus_tag="VFK23_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HET7318110.1"
                     /translation="MNNDIPKEIERFDLSARIQHVIMFTTFLLLSFTGWGLKYAYQDP
                     ASAWIRFWGGAKSAGIIHRVAGITMLLDFIYHQFYLINRVRKGGFRLSLVPMPKDVVD
                     LFHNFMFFFGLRKERPYFGTFNYMQKFDYWAVYWGMFIIGASGFFLAFPVTVSSIFPA
                     STVQWIWDVLFAMHSDEALLAIVFILIFHFYNEHLRSDVFPMNWMWLTGKMTTEQLKH
                     HHPAEYDRLYGNKKR"
BASE COUNT          648 a          804 c          826 g          554 t
ORIGIN      
        1 aaatccgaag gacgcggatg cctggtatca tctcgcggac gtctatgagc gtactgcaag
       61 ttaccgggaa gcggcggacg ccctcaacaa ggtcgtcgcc ctcgatcccg gcaggggttc
      121 tgcctacctc aaactcggga cggtctacaa cagactcggc cgccaccagg aagcggtaac
      181 gaacttccag aaggcgacaa aattcttccc caaagatccg gtcctgcata ataaccttgc
      241 cgtatcctac ggcaagctcg gcaagctgaa cgaggaaata aaactgctgg aaaaggcgat
      301 ttccctgcgt ccgcattacg cgacagcccg gtacaatctt gcgatggcct tcctgaagca
      361 ggggaagcgc gatcaggcgc tgaagcagta tgctgaactc gagaaattcg acgcgggcac
      421 ggcctcggcc ctgaagaaag aaatcgagaa aaataggtag gacctctcgt gagatattca
      481 cgcacaatca ttgcgcaggc gctcttctcc gccgtcgtga tgatctccgc ggccctcgca
      541 gccgacgttc cctgtttcca atgcggaatg atcgtggact tcgggtcaaa gttcacagcg
      601 aagatcgccg cagcggacag gacgctctta ttctgcgata tcggcgacct gttcgcctat
      661 ctgaagagaa aaaaactgag cgccgacggc gctttcgtaa aggattatcc cagcagcgaa
      721 tgggtcgagg cgaaggcggc gtacttcgtt catgacgaaa aaaaattcaa gacccccatg
      781 ggctggggcg tcgcggcgtt ccgtgaccgg gggcgcgccg cggaagcggg aaaggtcatg
      841 gactgggacg ctgcggcaag ggcgatgcag tgaagaaact cgtcgcgctc accgcgctgg
      901 catccggcgt cctgcttgtg ctcgtaccga ggttcatcct cccggcttgt gaatatgaag
      961 gatatcccgc aatgcactgc tcggacacgg cccgggcgga atatctcatc ggcgcactgc
     1021 tcgtcctggt cggcggcgcg gccttcttgg cgaaaacggc caaactttcg ctcacgggcg
     1081 ccgcagccgc cctcattctc tacgccgccg cctatctgct tcccgacagt atcggatact
     1141 gccggagccc tcggatgccg tgcaactacg gcatggttcc cgcggtgcgg ttcattgctg
     1201 ccgttggagc ggccacgatg atcatcgcct gcgccggcgt cgtgaatagc gtcagaatga
     1261 aaggaaaggc atgattgagc tgaaggggtt gtccaaatcg tacacgatat cggggaagac
     1321 catcaaggcc gtgcagcacg ttgatttgag catagcacag ggagaattcg tctccatcgt
     1381 ggggcattcg ggaagcggca agagcacgtt cctgagcctc gtcggcggca tcgcacggcc
     1441 cgatgacgga acagtcagca tcgacggaac ggacatctgg aaatacagcg acgtacagct
     1501 ttcaaagctc agaaacgaca agttcgggtt cgtctatcag ttcgcgagcc tcatccccac
     1561 cctcaacgcc gcggagaacg tccttcttcc caccgtattc ggcgggaaaa agacgcgggc
     1621 cgacgcggtt gcactgcttg agcgggtcgg tttgggcgat aaaacggacc gatacccttc
     1681 ggagctctcc ggcggagagc aacagcgcgt tgccatcgcc cgtgcgttca tcaatgatcc
     1741 gatgatcgtt cttgccgatg aacccacggg cgacctcgat gaggagacgg aagcggaaat
     1801 catggcgttc ttcgagaaga tcaaccggga gaggaaggtc accatgatca tggtgaccca
     1861 cagttcggaa ctcgccctcc gcgccccttc gcggctcagg atgaaacacg gtgtccttga
     1921 aaaactgacc tgatctgcct tgcccacggg tccgggcgga aaagacgtct tacgtgcgca
     1981 gaaaaaaacg gcggcctttc ggtcgccgtt tttaactttc acccgtttga tgcgtttctt
     2041 tcctaccttt tcttattccc gtaaagtcgg tcatactccg caggatggtg atgtttgagc
     2101 tgttcggtcg tcatcttgcc ggtgagccac atccagttca tggggaacac gtctgaacga
     2161 aggtgctcgt tgtagaaatg gaagatgaga atgaacacga tggcaaggag cgcctcatcg
     2221 ctgtgcatgg cgaacaggac gtcccagatc cactggaccg tcgacgccgg gaagatcgac
     2281 gatacggtca ccgggaaggc gaggaagaac cccgacgcgc cgatgatgaa catgccccag
     2341 tacaccgccc agtagtcgaa cttctgcatg tagttgaacg tcccgaagta cggcctctct
     2401 ttccggaggc cgaaaaagaa catgaaatta tggaacaggt cgacgacgtc cttgggcatg
     2461 ggtacgaggc tcagacggaa cccgcccttg cgtacccggt tgatcagata gaactggtgg
     2521 tagataaagt cgagcagcat cgtgatgccg gcgacgcggt gaatgatccc cgcgctcttg
     2581 gctccgcccc agaagcggat ccaggcgctc gccgggtcct ggtacgcgta cttgagcccc
     2641 cagccggtga atgacaggag caaaaatgtc gtgaacatga tgacgtgctg gatcctcgcg
     2701 gagaggtcga atctctcgat ttccttcggg atgtcgttat tcatgcttgc ccccctttct
     2761 gaaaagcttc tcgcggatgt ccgcgaaaat atccaaaaga acgtggatgc agatgaacgc
     2821 gaacacgctc at
//
LOCUS       DASSLD010000169         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_506920,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000169 DASSLD010000000
VERSION     DASSLD010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506920"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..769
                     /locus_tag="VFK23_03130"
     CDS             146..769
                     /locus_tag="VFK23_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7318111.1"
                     /translation="MRLVIIEDDPLLLESLALLLGGEEGITVTGAYRSAEDAMKALGS
                     AKPEVMLTDLGLPGMSGVELIKKAKEEMPSLEIMAHTVFEDRERVFSAIKAGASGYIL
                     KGSSPREIIESIHSLYKGGAPMSPKIARKIIHEFHDDAAGEPFLLTQREIEIVKLVEE
                     GLTYKEIGERLSISTHTVHTHIKNIYEKLQAKGRNDAIIKARKKGII"
     gene            1110..1484
                     /locus_tag="VFK23_03135"
     CDS             1110..1484
                     /locus_tag="VFK23_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7318112.1"
                     /translation="MARILIVDDENLILYSLSAALRNDGSQVTAVTNGKDALSEIRSS
                     LFDICFLDIHLPDANGFDLMKIVLEISPATRIILMTAIDLDDVQMNYLRHNNGHYLPK
                     PFDLEEVRSIVKGIQADLASTV"
     gene            complement(1505..>2166)
                     /locus_tag="VFK23_03140"
     CDS             complement(1505..>2166)
                     /locus_tag="VFK23_03140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7318113.1"
                     /translation="TGLAHEIKNPLAGIKVTMEILAEEPHLSDENRDMLAKVIGEIKR
                     IEYLMKGLLNFAKPPRPQLAKTDVNAVLETVASLALKDRTPSRDGSHAVHVVKDYQQD
                     IPKVLADPMQLQQILMNLVLNAADAMPQGGTVFLKTHFSRASNSLHIDIADTGTGIDA
                     AVMESIFKPFFTTKPKGTGLGLAITKRLIEENGGSISIENRAEGGALFKIILPPTPDQ
                     A"
BASE COUNT          482 a          616 c          602 g          466 t
ORIGIN      
        1 ggaaaatatt gagcaacccc ggggatcaat gctgtcgccg gcgtgtcgaa tacgagggca
       61 accctgcggg ggtcgaaatc cccctcccca taaaataccc cgacaagggg atggaaatat
      121 agcccggttt gtggtaggtt atgtcatgcg tctcgtgatt atagaagatg atcccctgct
      181 tctggagagc cttgcgctgc ttttgggcgg ggaggagggg attacggtca ccggcgccta
      241 ccggtcggct gaagacgcca tgaaggccct cgggagtgcg aagccggagg tgatgctgac
      301 cgatctgggg ctgcccggca tgtccggcgt cgaactcata aaaaaggcga aagaggagat
      361 gccctctctc gagatcatgg cccacaccgt ttttgaagac cgcgagcggg ttttttccgc
      421 gatcaaggcg ggcgcctcgg gctacatctt gaaaggaagc agcccgcggg agatcatcga
      481 atccatccac tccctgtaca agggcggcgc gcccatgagc ccgaagatcg cgcgaaagat
      541 catccacgaa ttccacgacg atgctgcggg cgaaccgttc ctgttgaccc agcgcgagat
      601 cgagatcgtc aagctcgttg aggaagggct gacgtacaag gaaatcggag aacggttaag
      661 cataagcacc cacaccgtgc atacccacat caaaaatatc tacgaaaaac tccaggccaa
      721 gggcagaaac gacgccatca tcaaggcaag aaagaaaggc atcatttaat cccgcatgaa
      781 cagcaatcgt gccgtcctca gctccccccg cagcagacct tacgccgatt gtcgaaaacg
      841 tccgagccgc actaccggcg ccggctttaa gggtcgacca tccgcataat tgagtcagag
      901 aggaaaacgc ggacaaagta cgtggcgact tccatcgtgg tgaaatgacg catgtcgtgg
      961 gtgattttcc catattctga ggctccgcgg tgccacgccg gtccgggaag tttccaaaat
     1021 ccccgggaat acgggaatcc gtcatcgagt atgaaatttg catgaataat aacggtggga
     1081 atacgtattc ctgcggacat tcaaacacta tggccagaat tttaatcgtt gacgacgaga
     1141 atctgattct ctactccctc tccgcggcgc tccggaacga cggttcgcag gttacggccg
     1201 tcacaaacgg gaaagatgcg ttgagcgaaa tacggagttc gcttttcgac atctgctttc
     1261 tggacattca tctccccgac gcaaacggct tcgatctcat gaaaatcgtg ctggagattt
     1321 cgccggctac caggatcatt ctgatgacgg caatcgatct cgacgacgtg cagatgaact
     1381 atctacgcca taacaacggt cattatcttc ccaagccctt cgatctggag gaagtgcggt
     1441 ccatcgtcaa gggtatccag gccgacttgg cgtcaacggt atgagatgcg taggcttgcc
     1501 tgcctcatgc ctgatccggg gtcggcggca ggatgatctt gaagagcgcc cctccttctg
     1561 ccctgttttc gatcgaaatg ctccccccgt tctcctcgat caatcgcttc gttatcgcca
     1621 gccccaggcc cgtccccttc ggtttcgtcg tgaagaaggg cttgaaaatg ctctccatca
     1681 ccgccgcgtc tatgccggtc cccgtatccg ctatatcgat atggagggaa ttcgaagccc
     1741 tgctaaagtg cgtttttaga aacaccgtcc ccccctgcgg catggcgtct gcggcgttca
     1801 ggacgaggtt catgagtatc tgctgaagct gcatcggatc ggccagcacc ttcgggatgt
     1861 cctgctggta gtccttgacg acatggaccg catgcgaccc gtcccgggag ggtgttcggt
     1921 ctttcagcgc caggctagcc acggtttcga ggacggcatt cacatcggtc ttggcgagct
     1981 gcggccgcgg cggtttcgcg aagttcagaa gtcccttcat cagatattct attcgtttga
     2041 tctcgccgat caccttggcg agcatgtccc ggttttcatc cgacaggtgc ggctcctcgg
     2101 cgagaatctc catggtgacc ttgatgcccg ccagcggatt cttgatctcg tgcgccaggc
     2161 ctgtcg
//
LOCUS       DASSLD010000170         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_248436,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000170 DASSLD010000000
VERSION     DASSLD010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248436"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFK23_03145"
     CDS             <1..235
                     /locus_tag="VFK23_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014797661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318114.1"
                     /translation="NDLVGSTDKALYEFKGGHVGVFVGTKSQKELAPAISQWLHERSG
                     NGAKKPEPPVQDSPRVKGAVRKKAAGKEKGHSE"
     gene            415..1158
                     /locus_tag="VFK23_03150"
     CDS             415..1158
                     /locus_tag="VFK23_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019693342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7318115.1"
                     /translation="MELTGKKVVVTGGARGMGRKFALDLKAQGAKPFVVDVVQENLDA
                     LEKEAGIPGQVVDVGSEKSVEAFFEKFTAENGAPDVLINNAGITADALFIKQKGEEIT
                     KFPFSNWERVLNVNLTGVFLCAREAAYHMVKHKVKGVIVNISSISRGGNAGQTNYSAA
                     KSAVDAMTVTWAKELSRYGIRVGAVAPGYINTEMVAKIRPDVLEKLVQNIPVGRLGEM
                     EEISQAVQFIIRNNFFTGRILEVDGGMRI"
     gene            1266..>2188
                     /locus_tag="VFK23_03155"
     CDS             1266..>2188
                     /locus_tag="VFK23_03155"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET7318116.1"
                     /translation="MEAYIIGGVRTAIGRFNGSLTPLDAIDLGSAVIKEVVKRTAVKK
                     DAVDEVIMGNVLQAGLGQNPARQASIRAGLPENVPAFTVNKVCGSGLKTVVLAGQSIA
                     AGEADAIVAGGMEHMTNAPYVVKSQRWGQRLGNAEMIDTLVNDALWDTFYECHMGVTA
                     ENIAEKFGISRKDQDEFAATSQRKTEQAIKEGKFKDEIVPLRIKQEKGEDKIFDTDEH
                     PRFGTTCDALAKLKPAFRSEGTVTAGNASGINDGAAAVLVVSAEKMKEMKPAWAFKIR
                     GSSTAAIDPAFMGLGPIGACKNVISRSGITL"
BASE COUNT          534 a          573 c          710 g          371 t
ORIGIN      
        1 gaacgacctc gtgggaagca ccgacaaggc gctgtatgaa ttcaagggcg gccatgtggg
       61 cgtctttgtg ggcacgaaat cccagaagga actcgcaccc gcgatatccc agtggctcca
      121 cgaacggtcc gggaacgggg caaaaaagcc ggagccgccg gttcaggaca gcccccgggt
      181 aaagggcgct gtgcgaaaga aagccgccgg taaagagaaa gggcactctg agtaaccgta
      241 actgctcagg tatcaatatt tcgtcatccc cggggagtcc aggtgtttga gaactctgga
      301 ttccccccag ctttagtcat gcaaggcagg cttctaaaac gttcgggaat gacggctata
      361 ggagaagacc atatgaggca cgagcaacca gatattgaga gggagacacc aggtatggag
      421 ctcacaggca agaaggtcgt cgttacaggc ggcgcgcggg gcatgggaag gaagttcgcg
      481 ctggatttga aggcccaagg cgccaaaccc tttgtggtgg acgtagtcca ggaaaacctc
      541 gatgcccttg agaaggaagc cggcattccc ggacaggtgg tggacgtggg cagtgaaaaa
      601 agcgtggagg cttttttcga aaagttcacg gcggaaaacg gagcgccgga cgtgctgatc
      661 aacaatgccg gcatcaccgc cgacgccctt ttcatcaagc agaagggcga ggagatcacg
      721 aaatttccgt tttcgaactg ggagagggtc ctgaatgtca acctcacggg cgtctttctt
      781 tgtgcgcgag aggccgccta tcacatggtc aaacacaagg tcaagggcgt gatcgtcaac
      841 atctcctcga tcagccgcgg gggaaacgcc ggccagacca attattccgc ggcgaaatcc
      901 gccgttgacg cgatgaccgt gacctgggcc aaggagcttt cccggtatgg catccgggtg
      961 ggcgccgttg cgccgggcta catcaacacg gagatggtcg cgaagatacg gcccgacgtc
     1021 ctcgaaaagc tcgtgcagaa cattcccgtg gggaggctgg gagagatgga ggaaatctcc
     1081 caggcagttc aattcatcat caggaacaat tttttcaccg gcaggatcct cgaagttgac
     1141 ggcgggatga ggatttagtc aggtcggatg ccattctcgc cgaaatggaa agattcgcca
     1201 tgaagcacac gaaggttgcg aaggttacgg agtgacgatc tgagctgcgc aggaagagga
     1261 tatctatgga agcatatatc atcggcggcg tcaggaccgc catcggaaga tttaacggtt
     1321 cgctcacccc gctcgacgcc atcgacctcg gatcggcggt tatcaaagag gtcgtgaaaa
     1381 ggacggcggt caagaaagat gccgttgacg aggtgatcat ggggaacgtg ctccaggccg
     1441 gactggggca gaacccggcc aggcaggctt ccatacgggc gggcctcccg gagaacgttc
     1501 ccgccttcac ggtcaacaag gtatgcggat cgggcctgaa gacggtcgtg ctcgcgggcc
     1561 agtccattgc ggcgggtgaa gcggacgcca tcgtcgcggg cggcatggaa catatgacca
     1621 acgcccccta cgtagtgaag tcacagcggt ggggacagcg cctggggaac gccgagatga
     1681 tcgatacgct cgtcaacgac gccctgtggg acacgtttta tgaatgccac atgggggtca
     1741 cggccgagaa catcgcggaa aagttcggca tcagcagaaa agaccaggac gagttcgccg
     1801 caacgtccca gcggaagacg gagcaggcga tcaaggaagg caagttcaag gacgagatcg
     1861 ttccgctgcg catcaagcag gaaaagggcg aggacaagat tttcgacacc gacgagcacc
     1921 cccggttcgg caccacctgt gatgcgcttg ccaaactgaa gccggcgttc aggagcgagg
     1981 gaacggtgac ggccggcaac gcctcgggga tcaatgacgg ggcggcggcc gtgctcgtcg
     2041 tgtcggccga aaaaatgaag gaaatgaagc cggcctgggc gttcaagatc aggggctcgt
     2101 caacagcggc catcgatccg gcgttcatgg ggctcggccc catcggcgcc tgtaagaacg
     2161 ttatttcccg ctcggggata actttgaa
//
LOCUS       DASSLD010000171         3493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1061233, whole genome shotgun sequence.
ACCESSION   DASSLD010000171 DASSLD010000000
VERSION     DASSLD010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061233"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VFK23_03160"
     CDS             <1..1365
                     /locus_tag="VFK23_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET7318117.1"
                     /translation="YRDRDGYFFITGRSKEMIVLSTGKKIFPEELEKYYKKIPAIKEI
                     CLIQGERGIEAAVVPDFDYLRKMNLANSRETIAFAIEDLAKDLPPYKRIAGLKIFKDS
                     LPVTRLGKLRRSKVRELYERSGERAEERTPEAEAEMPADPVTKKLLACLGPFTSKKNI
                     VPDDNLELDLGLDSLARVELVVSVEKSFGISLPDSFGSEVFTVKDAATKIRELLASGP
                     VTGGQGVKMTWADILSQEPSEEMRGQLELDSGPLCSGGQHMLTLMLRCFLSTYGRLSI
                     RGTENLPEEGSFIVAPNHLSLADAPAVMCTLPWKITAQTFFLGTTDFFGGPVTSRIAK
                     LLHVIPVDMETRLSSAMQLSAHVLRRGKVLCVFPEGGRSRDGRIKEFKKGVGIIAREL
                     NIPVVPAAIRGTYEMLPTGRMLPKPAKVSVSFGRPIYPEGRDYDEIVKKLYEEVVKLQ
                     EEGK"
     gene            1452..2315
                     /locus_tag="VFK23_03165"
     CDS             1452..2315
                     /locus_tag="VFK23_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HET7318118.1"
                     /translation="MLSILRKIRIILEMIKFEHTIFALPFAFTGALLAARGIPSWGTI
                     LWITVAMVGARSAAMGFNRWADREIDAENPRTKTRALPRNLVSPGQVIVFIALSSAVL
                     VFAAYKLNPLCFYLSPLALGIVFLYSYTKRFTFLAHAFLGLAISGAPLGAWIAVTGRF
                     EAPAFVLALAVLFWLVGFDVLYALQDIEFDRKAGLHSIPQRFGVGRSLWISRASHAVT
                     MAALFWLASLLSLGWYYVAGVIISLCLIVYEHTLVREGDLSKLDFAFFNMNGYISVTI
                     FFFTLLDVLLR"
     gene            2625..3374
                     /locus_tag="VFK23_03170"
     CDS             2625..3374
                     /locus_tag="VFK23_03170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004140962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET7318119.1"
                     /translation="MNVRMEIISLCMVFAVSPVFGGEKTELKTDKDKLSYALGADMGS
                     NLKKFEIDIDADLYVKGMKDALSGNVLLTEQEIKNVIMSLQKDLQAKQQEKMKAQGEK
                     NKKEGEAFLAENKKKDGVKALPSGLQYKVITEGKGASPKATDTVTVHYKGTLTDGTEF
                     DSSYKRNAPATFPVNGVIKGWTEALQLMKEGSKWQLFIPASLAYGESGRAGIPPNSVL
                     IFDVELIKVGAKEKQEEKKMEEKKEEKKKEK"
BASE COUNT          791 a          972 c         1032 g          698 t
ORIGIN      
        1 taccgcgaca gggacgggta ttttttcatc accggcaggt ccaaggagat gatcgtcctc
       61 tccacgggca agaagatctt ccccgaagag ctcgaaaagt attataagaa gataccggcc
      121 atcaaggaaa tatgcctcat ccagggggag cgcgggatcg aggcggccgt ggtcccggat
      181 ttcgactatc tccggaagat gaacctggcc aactcgcgcg agacgatcgc tttcgcgatc
      241 gaggacctcg ccaaggacct gccgccgtac aaaaggatcg cggggctcaa gatattcaag
      301 gactccctgc cggtcacgcg cctgggcaag ctccgaagga gcaaggtcag ggagctctac
      361 gaacgatcgg gcgagcgcgc ggaagagagg acgccggagg cggaggcaga gatgcccgcg
      421 gacccggtaa cgaaaaaact gctcgcgtgc ctcgggccgt ttacctcgaa gaaaaacatc
      481 gtgcctgacg acaacctgga gctcgatctg ggtctcgact cgcttgcccg cgtggaactg
      541 gtggtaagcg tcgaaaaatc gttcggcatc agcctgcccg attccttcgg ctccgaggtc
      601 tttaccgtga aggatgcggc cacgaagatc agggaactgc tggcctcggg tcctgtcacg
      661 ggggggcagg gcgtcaagat gacctgggcc gatatcctgt cccaggaacc gtcggaggaa
      721 atgagggggc agctcgagct tgattcgggg cctctctgca gcggcgggca gcacatgctt
      781 acgctcatgc tcaggtgctt cctctcgacc tacggccgtc tgtcgatccg cggaacggaa
      841 aacctgccgg aagaaggatc cttcatcgtt gcgccgaacc acctgagcct cgccgatgca
      901 cccgcggtca tgtgcacatt accatggaag atcaccgcac agaccttctt cctcgggaca
      961 acggattttt tcggcggccc cgtcacgtcc cgtatcgcaa aactcctcca tgtgattccc
     1021 gtggacatgg agacgcggct ctccagcgcg atgcagctct ccgcccatgt tctgcgccgg
     1081 ggaaaggtcc tgtgcgtgtt ccccgaggga ggccgttccc gcgacggcag gatcaaggag
     1141 ttcaagaagg gcgtcgggat catcgccagg gagttgaaca tcccggtcgt ccccgcggcc
     1201 atccggggga cctatgaaat gctgcccacc ggcaggatgc tgccgaagcc ggcgaaggtc
     1261 tcggtgtcct tcggcaggcc gatatatccc gagggcaggg actacgacga gatcgtgaag
     1321 aagctctatg aggaagtcgt gaaactgcag gaagaaggga agtaaaccga agctgttgca
     1381 cattataagt taacaccgct caacccgcta gacccgtaac ccgaaaccaa caacatcatt
     1441 ttggtggaac gatgctgtcg atcctcagga aaatcaggat catcctcgag atgatcaaat
     1501 tcgaacatac gatcttcgcc ctgcccttcg ccttcacggg ggcgcttctt gcggcgcggg
     1561 gcattccgtc gtggggaacg atcctctgga tcacggtcgc catggtggga gctcgttcgg
     1621 cggccatggg tttcaaccgg tgggctgacc gggagatcga cgcagagaac cccagaacga
     1681 agacccgtgc tctgccccgg aacctggtgt cgccgggaca ggtcatcgtc ttcatcgccc
     1741 tgtcgtcggc cgtactggtc ttcgcggcct acaaactgaa cccgctctgt ttttatctct
     1801 cccccctggc gctcgggatc gtcttcctgt attcctatac caagcgcttt acgttccttg
     1861 cgcacgcatt cctggggctc gccatctcgg gcgcccccct gggggcctgg atagcggtca
     1921 cgggcaggtt tgaggctccg gcgttcgtgc tcgcgctcgc ggtgctgttc tggcttgtgg
     1981 ggttcgacgt actctatgcc ctccaggaca tcgagttcga ccggaaggcg gggctccact
     2041 ccataccgca gcgattcggg gtcggacgtt cgctctggat ctcccgggcg tcccacgccg
     2101 tgacgatggc cgcgctcttc tggctggcgt cgctgctctc tctcggatgg tactatgtcg
     2161 caggggttat tatttccctg tgcctcatcg tctacgaaca taccctggtc agggaaggcg
     2221 acctctcaaa gctcgatttt gcctttttca atatgaacgg ttatatcagc gtaacgatat
     2281 tcttctttac gctccttgat gtcctgctcc gataacgcct gctggtagtc ccttgtattt
     2341 tagcttcccc gcggctgcac agctgtcatt cccgcacctg cttcaatcat gctggggcag
     2401 gctccagcgg gaatccattg gcttttctga aggctaaatt caaaatggat tcccgagaga
     2461 accattcggg aatgacggta tggttgtcag atggtttttg ccccgggatt tcgatagaca
     2521 tgaacaatca ggcttccctc ccacccataa ttgacaaacg gccgaaaagc tgataagttc
     2581 gtaacattgc ccaaggaata atttcacgga taaaaggaga aagtatgaac gtacggatgg
     2641 aaatcatttc attatgtatg gtttttgcgg tgagccccgt attcggcggt gagaagacgg
     2701 agcttaaaac cgataaggac aagctgagct atgcgctggg cgcggacatg ggcagcaacc
     2761 tgaagaaatt cgagattgat atcgacgcgg acctctatgt gaaagggatg aaggacgccc
     2821 tttccggaaa tgttctttta acagagcagg agatcaagaa tgtcatcatg tccctgcaga
     2881 aggacctcca ggccaaacag caggaaaaga tgaaggcgca gggcgagaag aacaaaaaag
     2941 aaggcgaggc gttcctcgcc gaaaacaaga agaaagacgg ggtgaaggcc ctgccgagcg
     3001 gcctgcagta caaagtcatc acggaaggga agggcgcgtc ccccaaggcc acggacaccg
     3061 tgacggtcca ttacaagggg acgctcaccg acggcaccga gttcgacagc tcgtacaagc
     3121 gcaacgcgcc ggcgaccttc ccggtgaacg gcgtaatcaa gggctggacc gaggcgcttc
     3181 agctcatgaa agagggctcc aagtggcagc tcttcatccc tgcgagcctt gcctacggcg
     3241 agtccggcag ggcggggatc cccccgaatt ccgtcctgat cttcgatgtc gaactgatca
     3301 aggtcggggc gaaggaaaag caggaagaga agaagatgga agaaaagaag gaagagaaga
     3361 agaaggagaa gtaaggttct atccagggta agacgaagtt gagtcgacga gattgtcatt
     3421 cccgcgaaag cgggaatcca ttatgaattt atctttgtaa agccaatgga ttccgggtca
     3481 agcccggact gac
//
LOCUS       DASSLD010000172         5117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_17866,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000172 DASSLD010000000
VERSION     DASSLD010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17866"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1876)
                     /locus_tag="VFK23_03175"
     CDS             complement(137..1876)
                     /locus_tag="VFK23_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7318120.1"
                     /translation="MEKKTIRKRTLFNKDGSQTAKTLDVVLNNITQGIVVVGPDYSVL
                     AFNKQLETLLGLQEGVIAVGRDFRDILKVWADATGQTQNNLTRTLRELDLPKPFEIEF
                     PQTINGVKQWCRLAHNPLPSKVFVRTYADITATKKAAEQHRYDAFHDPLTGLPNRALF
                     MDRLQHVITASQRRQSDSNYAVFFLDMDRFKVINDSHGHTIGDQLLVAVGRRLADCIR
                     PGDTIARLGGDEFALLLQDINDPSYTASVAERIQEKLSVPLDIKGHKIFSSISIGIAL
                     GTESYERPEQVLRDADIAMYEAKARGNASYEIFDIKLHANILDRMQLVADLRGAVDTK
                     ELALHYQPIIDLKTQQLTGFEALVRWKHPKRGLIYPLEFIPLAEEQGLIYQIGAWILH
                     EACRELKLLQERHPAHPPLNMSINISGRQFSQQDLIGIISEVIAETGIDPHTLILEIT
                     ESMIMENVDAAMGTMKRLRNVGLQIHIDDFGTGHSSLSYLQLFPVSALKIDRSFISKL
                     TGKGDNQEIITHIVSLAKSLNFDVIAEGVEMEHQLANVKDLHCGYGQGFLFARPMAFS
                     DIDAWMQGQKHQI"
     gene            complement(2332..2697)
                     /locus_tag="VFK23_03180"
     CDS             complement(2332..2697)
                     /locus_tag="VFK23_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318121.1"
                     /translation="MPDTLLQRLASGTAALKDKAASKAERDVLAAYSRLMTTYEDGML
                     LCRYRNQLHQFQFVPQGRIYVSQELDPLVEKYGLATERHLYEPTGAYWRSIDGNSIRV
                     IWESAEVQTKNIENMVNYN"
     gene            complement(3114..4205)
                     /gene="ald"
                     /locus_tag="VFK23_03185"
     CDS             complement(3114..4205)
                     /gene="ald"
                     /locus_tag="VFK23_03185"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET7318122.1"
                     /translation="MIIGVTKEIKTEEFRVGLTPVGARELAESGHAVVVETGAGAGSG
                     FADDEYRRAGAEISGRETVFARADLIVKVKEPVPPEYPLLREGVAIFTYLHLAPNPEL
                     TKLLLSKKITGLAYETLEQGGRLPLLAPMSEIAGRMAPLMAAYYLQKFKGGSGVLPTG
                     VSGVRPGKIVVLGAGVVGANAARVGIGLGMETVVINKDTAGLTRIDEIFKGRVRTLPM
                     SAHTLEVEIADADIVIGAVLVPGGRTPVLITRRMLATMKRGSVIVDVSIDQGGCVETS
                     RPTTHDDPVYMVDGIVHYCVANMPGAYPRTSTLALTNATLPYIKTIAELGIEKAVQTD
                     PVIRSALNTYDGRIAHKALAESLGTAALR"
     gene            4378..4902
                     /locus_tag="VFK23_03190"
     CDS             4378..4902
                     /locus_tag="VFK23_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HET7318123.1"
                     /translation="MAEEKKETWLNYLALTTVVFAVCATLSTLKGGGYGSKAVLCQAQ
                     ASDQWSYFQSKSIKGYLYELQRDKLDLELKAGTRAAEEYRKKIEFYQNEIGRYKGEKE
                     DIKKKAEELEAQRNDAQRHSGAFGLAAMFLQIAILLSSIAALMKQKYFWFLGMAAGVF
                     GLVYFANGFFLFMK"
BASE COUNT         1091 a         1388 c         1415 g         1223 t
ORIGIN      
        1 aacagagagg ggtatttgtg gggtatcagt tatttaattc cagatgaagt cgtggttgtt
       61 gcatggaatg gtaacacaac agtacaggat gaagtactcg aagtattcgg ccgggaatcc
      121 tcaaccgggt gagatgttat atttgatgct tctgcccctg catccacgcg tcgatgtcag
      181 aaaacgccat ggggcgggca aagaggaaac cctggccgta tccgcagtgc aggtccttga
      241 cgttggctaa ttgatgctcc atttcgactc cttcggcaat cacgtcgaaa ttcaggctct
      301 tggccaggga tactatatga gtgatgattt cctgattgtc ccccttgccg gtaagtttgc
      361 tgataaaact gcggtcgatc ttgagtgcac tgaccggaaa gagctgcagg taactcaacg
      421 aagaatgccc cgtgccgaaa tcatcgatat ggatctgaag ccccacgttg cgcagccgtt
      481 tcattgtccc catggcggca tcgacgttct ccatgatcat gctctcggtg atttccaata
      541 tcaaggtgtg cggatctatg ccggtctcgg cgataacttc ggagatgatg ccgataagat
      601 cctgctgcga gaactgcctg cctgaaatat tgatgctcat gttgagcggt gggtgtgcgg
      661 ggtgccgctc ctgaagcagt ttcaactcgc ggcaggcttc gtggagaatc catgcaccga
      721 tctgataaat gagtccctgc tcctccgcca gggggatgaa ctccaggggg tagatgaggc
      781 cgcgcttcgg atgcttccag cggacgagtg cttcgaatcc cgtcaactgt tgtgttttca
      841 ggtcgatgat gggctgatag tggagagcaa gctctttagt gtcgactgct ccccgcaggt
      901 cggctacgag ctgcatcctg tccaggatat tcgcatgcag cttgatatcg aagatctcat
      961 aggatgcatt tccccgtgct tttgcctcgt acatcgcaat gtccgcgtcc cgcagaacct
     1021 gctccggccg ttcgtagctt tccgtgccta aggcgatgcc gatgctgatc gaagaaaata
     1081 ttttatggcc tttgatatcg agcggaacag acagtttttc ctggatccgc tcggcgacgc
     1141 tggccgtgta agacggatcg ttgatgtcct gcaggagaag ggcaaattca tcgcctccca
     1201 gccgtgcgat ggtatctccc ggccggatgc aatcggccag cctgcggccg actgcgacga
     1261 gcagttgatc gccgatcgta tgaccgtggc tgtcgttgat gaccttgaat cggtccatgt
     1321 cgaggaaaaa gaccgcgtag ttggaatcgc tctgccgccg ttgcgacgcg gtgatgacgt
     1381 gctggagccg gtccataaaa agcgcccggt tcgggaggcc cgtcagcgga tcatggaagg
     1441 catcataccg gtgctgttcg gccgcttttt tcgtcgcggt gatgtcggca taggtcctca
     1501 caaatacctt tgagggcaaa gggttatggg ccaggcggca ccattgctta accccgttta
     1561 tcgtttgcgg gaattcaatt tcaaagggct tgggcagatc aagctcccgt aaagtccgtg
     1621 tcaggttatt ctgtgtttgt cccgttgcgt cagcccatac cttgagaata tccctgaaat
     1681 cacggcctac cgcgatgacc ccctcttgca gccctaaaag cgtctccagc tgtttgttaa
     1741 atgccagcac ggaatagtcc ggcccgacaa cgacgatgcc ttgtgtaatg ttgtttaaaa
     1801 caacgtcaag agtcttcgct gtctgagagc cgtctttatt gaacaatgtt ctcttgcgaa
     1861 tagttttttt ctccataaca cctctcgccg cgggtaaagc ggtcataagc gcttcgaccg
     1921 atggttggtg ttaagcggct ttatcccgga gccgtccggt ccctcgatat acagggggtc
     1981 gtcccggtaa tccccgtggg aatcgaacgc cgctcagtgc gattaaaacc gaattatctg
     2041 cgacgaatat atgcctgatt cggcacgatg tcaacaacgg tgaatgggta tattttgggt
     2101 atgtgtaagc gattacggaa agtaacgctg gttcctgcga cgaatggcaa taccattccc
     2161 ggaagaacat tttgaggttt tgctgaatag gttggcgatt ctgtcgattc acctgttata
     2221 aggaccgtta tatgcaaaaa acggcgtgct cagccgtatc tacggctttt tcacattgcc
     2281 gcgtcagttc ggacagttga ttttgtccgg tcgaagaaaa ttgtaatgcc atcaattgta
     2341 attcaccatg ttttctatgt tctttgtctg gacctcagcg ctttcccaga tcacccttat
     2401 cgagttcccg tcaatactcc tccagtacgc cccggtgggt tcatacaagt gacgttctgt
     2461 tgccaggcca tatttttcaa ccagcgggtc gagttcctgg gacacgtaga ttcgtccctg
     2521 aggaacgaat tgaaattgat gcagttggtt ccgatatcgg cacagcagca tgccgtcctc
     2581 atacgtagtc ataaggcgcg aataagctgc cagcacgtcg cgttccgcct ttgaggccgc
     2641 tttgtctttt aatgcagccg tcccggaggc cagtctttgc agcaatgtgt ccggcacatc
     2701 acagggtttg tccgatctga gggagaccct caactcctgt acggttctgt ttaactcgga
     2761 gaattttttc ttgtctatcc cgggttccca tgaacaacct ggtccgaaaa aaatgatgac
     2821 cgataaaaat agaatgacca cgccaccctg tctcatgcac accctcattc caaaaaaatc
     2881 gtaattcgct gtcttaccca ccgtgttttt tgtttgaggc acaacggaag attatagggg
     2941 aatgaaagac ctctcaatag tgattccccg atgaggcgag aatactatac tttatcttaa
     3001 acaacaaggg gaaatatgaa caacaatggg gtgcttttct cccgcacagg ttgcggtata
     3061 gaccctttcc gtatccatcc cgtcagcttt ctcctgccgc cggtccgggg cggtcatcga
     3121 agcgccgcag tcccgagcga ctccgccagc gccttgtgag cgatccgtcc atcgtacgta
     3181 tttaacgcgc tcctgatgac ggggtcggtc tggaccgcct tttcgatgcc aagctcggcg
     3241 atcgtcttga tgtaggggag cgtcgcgttc gtgagcgcca gggtcgatgt ccgggggtac
     3301 gcgccgggca tgttcgcgac gcagtagtgc acgattccgt ccaccatata gacggggtcg
     3361 tcgtgggtcg tgggacgcga ggtttcgacg cacccgccct ggtctatgga aacatcgacg
     3421 atcacggagc ccctcttcat cgtggcgagc atccgacggg tgatcaaaac cggcgtcctg
     3481 ccgccgggca ccagcaccgc gccgatgacg atgtctgcgt ccgcgatctc cacctccagg
     3541 gtgtgcgccg acatgggcag ggtccgcacg cggcccttga agatctcgtc gatccgcgtg
     3601 agaccggccg tgtccttgtt gatcacgact gtttccatgc cgagcccgat gccgacgcgg
     3661 gctgcgttgg cgccgacgac cccggcgccg agaacgacga tctttcccgg gcgaaccccg
     3721 gagacgccgg tgggaaggac gcccgatccc cccttgaact tctggaggta gtacgcagcc
     3781 atcaaagggg ccattctgcc cgcaatctcg ctcatggggg caagaagcgg aagcctgccg
     3841 ccctgctcga gagtttcata ggcgagaccg gtgatttttt tcgagagcag gagcttcgtc
     3901 agctccgggt tcggcgcgag atggagatag gtgaagatgg cgaccccctc gcgcaggagg
     3961 ggatactccg ggggcacggg ttccttcacc ttgacgatca ggtccgcccg ggcaaagacc
     4021 gtttcccggc cggaaatttc agcgccggcc cgccggtact cgtcgtcggc gaaaccgctc
     4081 ccggcaccgg cgcccgtctc caccacgacc gcatggccgc tttccgcgag ctcccgcgcg
     4141 ccgaccgggg tcagtcccac gcgaaactct tccgtcttta tttccttggt cacgccgatg
     4201 atcatgcgcc gttccttata agtaaaaggc atcaaaacat gcaaaaaatc gtgcttacta
     4261 aaatggtagc atatactccg cctgtctgcc gttcagggac atagggggct tgactagcaa
     4321 atcggatttc ggtagtatgt ggcctcatca aaatcaactt tcagcggagg atttttcatg
     4381 gcggaagaaa agaaagagac ctggcttaat tatctggcgc tcaccacggt ggtcttcgcc
     4441 gtttgcgcga cgctctcgac cctgaagggc ggaggatacg gatcaaaggc ggtgctgtgc
     4501 caggcgcagg cctcggacca gtggtcgtac tttcagtcca agagcatcaa ggggtatctg
     4561 tacgaattgc agcgggacaa actcgacctg gaactcaagg ccgggacccg cgccgccgaa
     4621 gaataccgga agaagataga attctatcag aacgaaatcg gccgttacaa gggtgagaag
     4681 gaggacatca agaaaaaggc cgaagagctc gaagcacagc gaaatgatgc ccagaggcat
     4741 tccggggcat tcggcctggc agccatgttc ctccagatcg cgatcctctt atcatccatc
     4801 gcggccctca tgaagcagaa atatttctgg ttcctcggca tggcggccgg ggtcttcggc
     4861 ctggtctatt ttgcgaatgg gtttttcctg tttatgaaat agggaagctg cgtttcgggt
     4921 tgcgtggtgc gagttgcgag atatgcgttg caggttttaa cccgtacccc gcaacgcgca
     4981 atccgcaaca ttttttcttc atttcttaaa gtccaccgcc tggcctttct gcaggacgtc
     5041 gatgcttttg cgcaccatgg cagtcgacgt gcgttccttc acaagaaaca cctggacctc
     5101 gccgaccatc tccctcg
//
LOCUS       DASSLD010000173         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_572103,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000173 DASSLD010000000
VERSION     DASSLD010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572103"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="VFK23_03195"
     CDS             <1..1172
                     /locus_tag="VFK23_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET7318124.1"
                     /translation="QHLLKGVKIRVYPVGRLDYNTEGLLLLTNDGDFAHLITHPKHEF
                     PKTYRAKVKGVLEDRQIDELEKGIYLDDGKTAPAKVKKLSKEEANSWLEITIHEGRKR
                     QVRRMFDRVGHSVIKLKRVRTGNLMLGDLPEGSFRYLTSDEVQAMKELATKEWSGAAG
                     AREDQVKKREGSEPRGKGPAMAVGLRSREARVPRTGPSMDRDHKGEGKPFRPSFTPSV
                     KDGAVSSPLRVRGKFVAGEGRTGGAVRGAEGRRQTQEPRVPGQGASGRGPRRPLRDTG
                     NRRSQGAGFTGPKAGSPVFKEKSRMRTATGHPGHEESDRGVSKSSAFRPQGKKTTVAR
                     MRGKIFDTGPSRKPGTERPASPRAGDRKPARTFERHEIRKSTLQGASRKPYHTKK"
     gene            1208..1540
                     /locus_tag="VFK23_03200"
     CDS             1208..1540
                     /locus_tag="VFK23_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET7318125.1"
                     /translation="MVRKISTSPAKGKDAEHLRAQVIIHGIVQGVFFRAATRDEARRI
                     GVGGWVRNLPDGTVQALFEGEKKKVEEIIGWCHKGPPGAQVTRVDISWEPYQGEYRQF
                     DIRYGYID"
     gene            1583..>2011
                     /locus_tag="VFK23_03205"
     CDS             1583..>2011
                     /locus_tag="VFK23_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7318126.1"
                     /translation="MKTAMTILIGAILLVVALSLLLIYSNTHPPKYPLYVPPSQYKAD
                     YEDVVFTSEDGVKLTGWLVKPPLPHSPSSPAIIICHGVGANKSDFTEFAIALSRRGYE
                     VLLFDFRAHGGSGGSRTSLGYREQKDVLAALGYLKTRLEID"
BASE COUNT          478 a          567 c          637 g          329 t
ORIGIN      
        1 tgcagcacct gctcaaggga gtcaagatcc gggtttatcc cgtcggccgg ctggactaca
       61 acacggaggg gctcctgctc ctgacgaacg atggcgactt cgcgcacctg atcacgcacc
      121 ccaagcacga attccccaag acctaccggg caaaggtcaa gggcgtgctc gaagaccgcc
      181 agatcgacga actcgaaaaa ggcatctacc tcgacgacgg aaagaccgct ccggcgaagg
      241 tcaagaaact gagtaaggaa gaggcgaatt cgtggcttga aatcacgatc cacgaaggaa
      301 ggaaacggca ggtacggcgc atgttcgacc gcgtgggcca ctcggttatc aaactcaagc
      361 gcgtcaggac cggaaacctc atgttgggag accttcccga ggggtcgttc cgctatctga
      421 cttcagacga ggtccaggcg atgaaagaac tggcaaccaa agaatggtcg ggtgcagccg
      481 gggcgcggga agatcaagtc aagaagcggg aggggtcgga gcccaggggc aaggggccgg
      541 caatggccgt aggactgcgc tcgcgggagg cgagggtccc gaggacagga ccatccatgg
      601 accgtgatca caaaggtgaa ggcaagccct tccgaccttc ctttacccct tccgtcaagg
      661 acggggcagt atcctctccc ctccgtgtga gaggaaaatt tgttgcgggg gaaggccgga
      721 ccggtggcgc tgtgcgggga gctgaagggc gcaggcaaac ccaggaaccc agggtacccg
      781 ggcagggggc gagcggcagg ggtccgagac ggcccttgag ggacacaggc aacaggagga
      841 gccagggtgc cggattcacg ggccctaaag cagggagtcc ggtgttcaaa gagaaaagcc
      901 ggatgcgcac cgcaacgggg catccggggc atgaggaatc ggaccggggc gtgtctaaga
      961 gttccgcgtt cagaccgcaa ggcaagaaaa ccactgtggc cagaatgcgc ggaaaaatat
     1021 tcgacacggg tccttcacgg aaaccgggta ccgagcggcc tgcaagtccg cgtgctggag
     1081 atcgtaagcc tgcgcgcact tttgagaggc acgagatccg gaaatcgacg ctgcagggtg
     1141 ccagccgcaa gccctatcat acgaaaaagt aggcctcccc gggcggaaga agagcgcgag
     1201 caagaacatg gtcaggaaga tttcaacgtc acccgcaaag gggaaggacg cagaacacct
     1261 tcgcgcacag gttattatcc atggaatcgt ccagggggta ttttttcgcg ccgccacccg
     1321 ggacgaggca agacggatcg gcgtgggggg gtgggtcagg aaccttcccg acggcacggt
     1381 ccaggccctc ttcgagggag agaagaagaa ggtcgaggag atcatcggat ggtgtcacaa
     1441 aggcccgcct ggagcgcagg tgacccgggt ggacatctcc tgggagccgt accagggaga
     1501 gtacagacaa ttcgacatcc ggtacggcta tatcgattag atacggtact tctcatatcc
     1561 gacacgtcat caacgacttc tcatgaaaac cgccatgacc atcctgatcg gggccattct
     1621 actcgtcgta gcgctctcac ttctgctgat ctattcgaac acccacccgc cgaaatatcc
     1681 gctgtacgtg cctccctcgc aatacaaggc cgattatgag gacgtcgttt tcacgagtga
     1741 ggacggcgtc aaactcacgg gctggctcgt caagccgccc cttccacact cgccgtcgtc
     1801 tcctgccatc atcatctgcc acggcgtcgg cgcgaacaaa tcggatttta ccgagttcgc
     1861 aatagccctt tcgcgccggg gatatgaggt cctcctgttc gactttcgcg cccatggagg
     1921 gagcggtgga agccgaactt ctctcggtta ccgcgagcag aaggacgttc tcgccgcgct
     1981 tgggtatctg aaaacgcggc tggaaatcga t
//
LOCUS       DASSLD010000174         3691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1094327, whole genome shotgun sequence.
ACCESSION   DASSLD010000174 DASSLD010000000
VERSION     DASSLD010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094327"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..802
                     /locus_tag="VFK23_03210"
     CDS             47..802
                     /locus_tag="VFK23_03210"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET7318127.1"
                     /translation="MRLVRFNAGDRTACGVLDGDEVVELAGDFFGPLKTLTTRHRLSA
                     VRLLAPCVPGKIVAVGLNYRDHAAELGLAVPAEPVIFLKPATAVVGPGGDIRCPPMSA
                     QVDFEAELGVVIKDRVKAVGPGEAAEHILGYTCANDVTARDLQKKDGQWTRAKSFDTF
                     CPVGPWIETELDSGDLLIESFLNGDRRQSSRTSRFIFPVPFLVSFISQIMTLLPGDLI
                     ITGTPAGIGPMRPGDEVEVRIEGIGSLRNRVTR"
     gene            986..2227
                     /locus_tag="VFK23_03215"
     CDS             986..2227
                     /locus_tag="VFK23_03215"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HET7318128.1"
                     /translation="MTASETYIQSLFADRIGGKMFGKEQKIYKFEKIKRAKRAALAAN
                     PGKELIDFGVGEPDEMAFPGVVSELRYEAEKPENRGYADNGIQEFKDAAARYLLKVYG
                     VGGIDPQTEVLHGIGSKPVLAMFPSIFINPGDVTLMTTPGYPVMATHTRWYGGEVVNL
                     PLVEEKGFLPDLDGLSPDIKKRAKLLYINYPNNPTGAGATWTFFEKVVLFAKANNVVV
                     VHDAAYAALTYGKKPLSFLSVPGAKEVGVEIHSLSKAFNMTGWRLAFVAGSERMIAGY
                     GNVKDNYDSGQFKAIQKAGVYALDHPEITDEIAAKYERRLRAMTAVLADMGFHAKMPA
                     GTFYLYVRMPKGVRNGRAFANAEEFSEFLIAEKLISTVPWDDAGPYIRFSATFECKGR
                     EDEQRVLDEFKKRMSGLQFEF"
     gene            2323..2808
                     /gene="folK"
                     /locus_tag="VFK23_03220"
     CDS             2323..2808
                     /gene="folK"
                     /locus_tag="VFK23_03220"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET7318129.1"
                     /translation="MPITAYIGLGSNMGDKMATCRTALDLLSRAGRVARVSSFYCTEP
                     VGYADQEPFLNAVAELETDLSPLALLAACHVIEEELGRNRLFRWGPRTIDLDILLYGD
                     QVVDTGELTVPHALMAERRFVLAPLNEIGPDVVHPVSRKTVSTLLKELRDSHKVVKCE
                     E"
     gene            2946..3614
                     /locus_tag="VFK23_03225"
     CDS             2946..3614
                     /locus_tag="VFK23_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HET7318130.1"
                     /translation="MTAKPAPSLHTKYIVTEGPIGVGKTSLTTLLADELGARLVLEQA
                     ENNPFLTDFYKDPQRYRFQTQMFFLLNRFSQQQEMLQPDLFTRITISDYIFDKDRIFA
                     YLTLDEHELPLYEQLYKVLEPKIMKPDLVIFLQADTDTLLKRIKSRGRSFEKEVSRDY
                     IAAVNEAYNQFFFRYGDTPLLVINTSDIDFVHRREDLDDLLRQILSMKQGTQYYVPRS
                     RGKK"
BASE COUNT          809 a         1062 c         1115 g          705 t
ORIGIN      
        1 ggaagaaaag ctcctggagt ggatcgacaa gaagcaataa cgctggatga gactggtacg
       61 gttcaatgcc ggggacagga cagcgtgcgg cgtcctggac ggcgacgagg tcgtggaact
      121 cgcaggggat tttttcgggc cactgaagac gctcaccact cgccaccgac tttccgcggt
      181 ccggcttctg gcgccgtgcg tgccgggaaa gatcgtcgcc gtgggcctta attaccggga
      241 ccatgcggcg gaattggggc tcgcggtccc cgctgagccc gttattttcc tgaaacccgc
      301 gaccgcggta gtcggtcccg gcggcgacat ccgctgtccc ccgatgagcg cgcaggtgga
      361 tttcgaggcg gagctgggcg tcgtcatcaa ggaccgcgtg aaggcggttg ggcctggcga
      421 ggcggcggag catatcctcg gctacacctg cgcgaacgac gtgacggcgc gggaccttca
      481 gaagaaggac ggccagtgga caagggccaa gtccttcgat accttttgcc ctgtcgggcc
      541 gtggatcgag acggagctgg attccggcga ccttcttatc gagtcctttc tgaacggcga
      601 ccggcggcag tcgtcgcgga cgagccggtt catctttccg gtgccctttc tcgtgagctt
      661 catttcacag atcatgacgc tgttgcccgg ggacctcatc atcaccggga cgcctgccgg
      721 catcggtccg atgaggccgg gggacgaggt ggaggtgagg atcgagggga tcgggagttt
      781 gaggaacaga gtcacgagat aagcattttg gacttaaccg cgaataggcg ctaatgatca
      841 ccaataagct tgaggtcaaa agataaggca tttttggacg cagataaacg cggaagaatc
      901 ggatttacgc tgattaaaaa aatctgcatt catccgcgct gatcagcttc cgactatatt
      961 tgatttcgga ctggaggaac aacggttgac tgcaagcgaa acctacatcc aatccctttt
     1021 tgccgaccgg atcggcggaa agatgttcgg caaggagcag aagatctaca agttcgagaa
     1081 gatcaagcgc gcaaagcgcg cggcccttgc agcgaacccg ggaaaggagc tgatcgactt
     1141 cggcgtgggc gagcccgacg agatggcctt ccccggcgtg gtcagcgagc tccggtatga
     1201 ggcggagaag ccggaaaacc gcggatatgc tgacaacggc attcaggagt tcaaggacgc
     1261 ggcggcccgg tatctgctga aggtctacgg cgtcggcggc atcgaccctc agaccgaggt
     1321 gctgcacggg atcggcagca agcccgtgct cgccatgttc ccgtcgatct ttatcaatcc
     1381 cggagacgtg acgctcatga cgacgccggg ctatcccgtc atggcgaccc acacgcgctg
     1441 gtacggagga gaggtggtga acctgccgct cgtcgaagag aagggctttc tgccggacct
     1501 ggacggactt tccccggata taaagaaaag ggcgaagctc ctctacatca attacccgaa
     1561 caatccgacg ggcgcgggcg cgacctggac attcttcgag aaggtcgtct tgttcgcgaa
     1621 ggcgaacaac gtcgtcgtgg tgcacgacgc cgcctatgcg gcgctcacct acgggaaaaa
     1681 gccgctttcg tttctctcgg tgcccggcgc aaaggaggtg ggcgtcgaga tccattcgct
     1741 ttcgaaggcc ttcaacatga cgggctggcg cctcgccttc gtcgcgggca gcgaacgcat
     1801 gatcgcaggc tacggcaacg tgaaggacaa ctacgactcc ggccagttca aggccattca
     1861 gaaggcgggt gtttacgccc tcgaccatcc ggaaatcacc gatgagatcg ccgccaagta
     1921 cgagcggagg ctcagggcaa tgacggccgt ccttgcggac atgggctttc acgcaaagat
     1981 gcccgcggga acgttctatc tctacgtcag gatgccgaaa ggcgtccgga acggaagggc
     2041 cttcgcgaac gcagaggagt tctccgagtt tctgatcgcc gaaaagctca tcagcacggt
     2101 cccctgggac gacgcggggc cgtacatccg gttctcggct accttcgagt gcaagggcag
     2161 ggaggacgag cagcgggttc tcgatgaatt caaaaagagg atgagcgggc tgcagttcga
     2221 gttttaaacg cgatgagcat agagcaaagc gcatagcgtt gaatcaatcc tgtttgatgc
     2281 atcctgcgcc atgcgctctg cgctctgcgg tttgaatatt tcatgccgat taccgcgtac
     2341 atcgggctgg gctcgaacat gggggacaaa atggcgacgt gccgaacagc gctcgacctt
     2401 ttgagcaggg ccggccgcgt ggcccgggtg tcgtcgtttt actgcacgga gccggtaggt
     2461 tacgcggacc aggaaccgtt tttaaacgcc gtagcggagc tcgagaccga cctttctccg
     2521 ctggcgctcc ttgcggcctg ccatgtgatc gaggaggaac tggggcgaaa caggctgttc
     2581 cggtgggggc cgcgtaccat cgatctcgac atcctcctgt acggcgacca ggtggtcgac
     2641 accggcgaac tcaccgtccc ccacgcgctc atggccgaga ggcggttcgt gctcgccccg
     2701 ttgaatgaaa tagggccgga tgtagtgcac cccgtatcca ggaagaccgt ctcaaccctg
     2761 ctcaaggaac tcagggactc gcacaaggtg gtgaagtgcg aggagtaagg cggcagcaaa
     2821 gtcagtcatc cccgaatgct tctatcgggg atctggtgtt tcctcaaacg tcacaaaacc
     2881 gtattcccga tttaggcgtt cgggaatgac aatctgcgga tctattgcag ataatcataa
     2941 aagtcatgac cgcaaaaccg gccccatctc ttcatacgaa atacatcgtc accgaaggcc
     3001 ccatcggcgt cggaaagacg agcctcacga ccctcctggc cgatgagctc ggcgcccgcc
     3061 tggtcctgga gcaggcggaa aacaaccctt ttctgacaga tttctacaag gacccgcagc
     3121 gctaccgctt ccagacccag atgttctttc tcctgaaccg cttcagccag cagcaggaga
     3181 tgctccagcc cgacctgttc acgcggataa cgatcagcga ttacatcttc gacaaggacc
     3241 ggatcttcgc ctacctcacc ctcgacgagc acgagctgcc gctctacgag cagctctaca
     3301 aggtgctgga acccaagatc atgaagcccg acctcgtgat cttcctccag gcggacaccg
     3361 acacgctgct taagcgcatc aagagccgcg gcaggtcctt cgaaaaagaa gtgagccgcg
     3421 actacatcgc cgcagtcaac gaggcgtaca accagttctt cttccgctac ggcgatacgc
     3481 cgctcctcgt catcaatacc tcggacatcg atttcgttca tcgccgtgaa gatctcgacg
     3541 acctgctcag gcagatcctg agcatgaagc agggcacgca gtattacgtg ccgaggtcga
     3601 gggggaaaaa ataaactccg tcctgagtcg acagtcgttg gtcgtcagtc ggtagacacc
     3661 tgtcattagt ttcaagtctt cccgctccta a
//
LOCUS       DASSLD010000175         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1196089, whole genome shotgun sequence.
ACCESSION   DASSLD010000175 DASSLD010000000
VERSION     DASSLD010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196089"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VFK23_03230"
     CDS             complement(<1..970)
                     /locus_tag="VFK23_03230"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7318131.1"
                     /translation="MLRRVFVLFVGGFSVAASAQAVDPLPSWNDGGAKRAIVEFVQAT
                     TTQGGPQFIPPAERIATFDQDGTLWVEQPVYTQVMYCLDRVPAVVKAKPELANVEPFK
                     TVLSGNREAIAKLPEAALLKIVAATLTGMSVDEFQSEVKRWLVDARDPRWKKPYTDLT
                     YLPMQEVMIYLRANGYKTYIVTGGGQDFVRVYAEATYGIPPEQVVGTAAGVQYAYGRD
                     GKPILTKEPKLLLNDDKAGKPEGIHLMIGRRPVVAFGNSVGDAQMLEYTKAGSGTRLA
                     MIVLHDDAKREYVYGPAAGLPISKVGTFPQATYDQATRQGWIVIRMK"
     gene            complement(1123..>2381)
                     /locus_tag="VFK23_03235"
     CDS             complement(1123..>2381)
                     /locus_tag="VFK23_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET7318132.1"
                     /translation="FTGKWHLGEADYALPSAQGYDEMRYAGLYHLNAYTYADPTWFPD
                     MDPELRDMFNKVTIGSLSGKAGEKAHEDFKINGQYVNTPEKGVVGIPFFDEYVEKAGI
                     EFLEKAAKDPSKPFFVNINFMKVHQPNMPHPDFIHKSMSKSKYADSLVENDTRIGHVL
                     DKIRQLGLDKNTLVVWTTDNGAWQDVYPDAGYTPFRGTKGTLREGGNRVPAIAWWPGK
                     IKPNTKNFDIVGGLDLMATFAKVGGVDLPKNDREGKPIVFDSYDMSPILFGTGKSQRK
                     SWFFFTENELSPGAARVGNYKAVFNLRGDNGQATGGLAVDTNLGWKGPEKYVATVPQV
                     FDLWQDPQERYDIFMNNYTERTWTLVTITDEIKNLMKTYVQYPPRKLQSEAYTGPITL
                     TGYERVQQVREMLMKEGISLPMPTGN"
BASE COUNT          389 a          780 c          700 g          512 t
ORIGIN      
        1 ccttcatgcg gatgacgatc caaccctgcc ttgttgcctg gtcgtacgtc gcctgcggaa
       61 acgtcccaac cttgctgatg ggcagtcctg ccgcagggcc gtagacgtac tcgcgcttcg
      121 catcgtcgtg cagcacgatc attgcgagcc gtgtaccgct tcctgccttc gtgtattcca
      181 gcatctgcgc gtcgccgacg gagttgccga acgcaaccac gggcctccgt ccgatcatca
      241 ggtggatgcc ctcgggcttc ccggccttgt cgtcgttgag cagcagcttc ggctccttcg
      301 tcaggatcgg cttcccgtcc ctgccgtacg cgtactggac gcctgcagca gtccccacca
      361 cctgctcggg cggtatcccg taagtcgcct cggcatagac gcgcacgaag tcctgtcccc
      421 ccccggtgac gatgtaggtc ttgtagccgt tcgcccgcag gtagatcatc acttcctgca
      481 tcggcaggta ggtaaggtcg gtatagggct tcttccagcg cgggtctctc gcgtcgacaa
      541 gccaccgttt cacctcgctc tggaattcgt ccacggacat ccccgtcagc gtagcggcga
      601 cgatcttgag cagcgcggct tcggggagct tcgctatagc ctcgcgattg cccgacagca
      661 cggtcttgaa cggctcgacg ttggcaagct cgggcttcgc cttgaccacc gccgggacgc
      721 ggtccaagca atacatcact tgcgtgtaaa ccggctgctc gacccagagc gtgccgtcct
      781 gatcgaatgt cgcaatgcgc tcggccggcg gaatgaactg cggaccgccc tgggtcgttg
      841 tcgcctgcac gaactcgacg atcgccctct tggcgccgcc gtcgttccag gacggcaagg
      901 ggtcgaccgc ctgcgcactg gcagcaacgg agaagccgcc cacgaatagg acaaacactc
      961 ggcgcaacat ggcgcccgcc tggaatttca tcatggttgc atcaagactc cattcggttt
     1021 cggtcatcaa agtcaaaagc cgcacttccc agaacgggaa ggcggccttt gctcacttag
     1081 cgcgactcct cgcgggttgg cccagggccg cccgcctccg cattagttac ccgtcggcat
     1141 tggcaggctg atgccttcct tcatgagcat ctcccggacc tgctggacgc gctcgtagcc
     1201 cgtgagcgtg atcggaccgg tatacgcctc gctctggagc ttgcgcggcg gatattggac
     1261 atacgtcttc atcaggttct tgatctcgtc ggtgatggtg acgagtgtcc aggtgcgctc
     1321 ggtgtagttg ttcatgaaga tgtcgtaccg ctcctgcggg tcctgccaca aatcgaagac
     1381 ctgcggaacg gtcgcaacat acttttccgg ccctttccag ccgagattgg tgtcgaccgc
     1441 cagccccccg gtcgcctggc cgttgtcgcc gcggaggttg aataccgcct tgtagttgcc
     1501 gacgcgggcg gcacccggcg agagctcgtt ctcggtgaag aagaaccacg acttccgctg
     1561 cgacttgccg gtcccgaaaa ggatcggcga catgtcgtag ctgtcgaaga cgatcggctt
     1621 gccttcgcga tcgttcttcg ggagatcgac cccgccaacc tttgcgaagg tcgccatcag
     1681 gtcgaggcca cccacgatgt cgaagttctt cgtgttcggc ttgatcttgc ccggccacca
     1741 cgcaatcgcc ggcacgcggt tgccgccttc ccgcagcgtc cccttcgttc cgcggaacgg
     1801 cgtgtaaccg gcgtccggat agacgtcctg ccaggcgccg ttgtccgtcg tccagaccac
     1861 cagcgtgttt ttgtcgaggc cgagctgccg gatcttatcc agaacgtgcc cgatccgcgt
     1921 gtcgttttcg accagcgagt cggcgtactt cgacttcgac atcgacttgt ggatgaagtc
     1981 cgggtgcggc atgttcggct ggtggacctt catgaagttg atgttgacaa agaacggctt
     2041 gctcgggtcc ttcgccgcct tctccaggaa ttcgatgccg gccttctcga cgtattcgtc
     2101 gaagaaggga atgccgacca cgcccttctc cggcgtattc acgtactgcc cgttgatctt
     2161 gaagtcctca tgcgcctttt cgcccgcctt gcccgacagc gacccgatcg tcaccttgtt
     2221 gaacatgtcg cgcagctccg ggtccatgtc cgggaaccac gtcgggtcgg cgtaggtgta
     2281 ggcattgaga tggtagagcc ccgcgtagcg catttcgtcg tagccctgcg cgctcggcaa
     2341 cgcgtagtcg gcctcgccga ggtgccactt gcccgtgaag a
//
LOCUS       DASSLD010000176         3678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_376421,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000176 DASSLD010000000
VERSION     DASSLD010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376421"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /gene="acnA"
                     /locus_tag="VFK23_03240"
     CDS             <1..736
                     /gene="acnA"
                     /locus_tag="VFK23_03240"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141279.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HET7318133.1"
                     /translation="FGGITSARALAIFGDSLTTDHISPAGSIRESVPAGKYLIENGVS
                     KSDFQSYGTRRGNHEVMIRGTFANVRIKNLMLPPGPDGSRVEGGLTLYQPSGERMSIY
                     DAAMKYMALKTPTIVFGGEEYGTGSSRDWAAKGTRLLGIRAVIARSFERIHRSNLVGM
                     GVLPLQFQGRDSVQSLGIRGDEAFDILGMDDLKPQQEATLVIKRRDGSRWEVKLLSRI
                     DTPAEVDYYRHGGILPYVLRELIAGA"
     gene            806..2707
                     /locus_tag="VFK23_03245"
     CDS             806..2707
                     /locus_tag="VFK23_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HET7318134.1"
                     /translation="MMGRYEELFQKSIKDPEGFWAQAAEAITWITSWNKVLDDSNKPF
                     YRWFSGAELNTCFNAVDRHAMFGRADQAAIIYDSPVTNTVQKITYRELLDLVSRFAGA
                     LANLGVQKGDTIIIYMPMIPQAVIAMLACARLGAVHSVVFGGFAPHELAIRIDDAKPK
                     IIITASGAVEGKKILEYKPMLDRAIEAAAHKPEKNIIFQRQFVQASLNPQTDLDWNEL
                     MAKAAPVECTPVKATDPLYILYTSGTTGKPKGVVRDNGGHAVALRWSMRYIYGVQPGD
                     VYWAASDVGWVVGHSYIVYGPLLHGCTTVLYEGKPVGTPDAGAFWRVISQHGVKVLFT
                     APTAFRAIKREDPKGEFVKKYDLSRFKALFLAGERLDPDTYHWAHNLLRKPVIDHWWQ
                     TETGWPITANCMGIEEFPIKPGSSTKPVPGFDVTILDPNGKKLGPKETGLVTIKLPLP
                     PGTLPTLWQADARFEESYLRVFPGYYFTSDGGYIDEDGYVFIMGRVDDVINVAGHRLS
                     TGEMEEVVSTHKDIAECAVIGVHDELKGQVPLGLCVLKSGSNATETAIRKDLSQMIRE
                     NIGPIASYKETCVVKRLPKTRSGKILRGTMRKIADGEPYTVPSTIDDPATLTEIEDAL
                     KHVGYAKKH"
     gene            2868..>3678
                     /locus_tag="VFK23_03250"
     CDS             2868..>3678
                     /locus_tag="VFK23_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7318135.1"
                     /translation="MKSGFKLGDFELTWLNGGRFELDGGAMFGVVPKVLWQKKYPANA
                     DNCIPLHAWPILIKTPDGLLLIESGLGNKLTDKQKKIYRVQEEWSIEADLKKLGLARE
                     DIDYVILTHYDFDHAGGVVMQEPDERLSLSFPKAKHIIQKKEWEDVLNPNIRSINTYW
                     PVNNELLRGSKNLELVDGEAGVVSGIRVVRTGGHNEGHQIVKMESKGERAYHLGDILP
                     THAHFNPLWVMAYDNYPMDSIAMKEKWIRSAVAEEAWFTFYHDAFVLVARFD"
BASE COUNT          824 a         1091 c         1072 g          691 t
ORIGIN      
        1 gttcggcggt attacaagcg cgcgtgcgct cgccatcttc ggtgactcgc tcaccacaga
       61 ccatatcagc cccgcaggct cgatccggga gagcgtgccg gcaggaaaat atttaattga
      121 aaacggcgtt tccaaatcag acttccagag ctacggcacg cgccgcggca accacgaggt
      181 catgatacgg gggaccttcg ccaacgtgcg gatcaagaac ctgatgctgc cgcccgggcc
      241 cgacggaagc cgcgtcgaag gcggtctcac cctgtaccag ccctcgggag aacggatgtc
      301 catctatgac gcggcgatga agtatatggc cttgaagacg ccgaccatcg tgttcggcgg
      361 agaggaatac ggcacgggtt catcgagaga ttgggccgct aaagggaccc gtctcctcgg
      421 tatcagggcg gtcatcgccc ggagcttcga gcgcatccac cgctccaacc tcgtgggcat
      481 gggggtcctg ccgctccagt ttcagggcag agactccgtt caatctctcg ggatcagggg
      541 cgacgaagcc ttcgatatcc tgggcatgga cgacctcaag ccgcagcagg aggcgacgct
      601 cgtcatcaag cgtagggacg gctcgagatg ggaagtcaag ctcctctcgc gcattgacac
      661 gcccgcggaa gtggattatt atcggcacgg aggcattctc ccctatgtcc tgagggagct
      721 gatcgcaggc gcataaaata aggccgtatt ttttttgtcg accgggtcgc aaaaatgtgc
      781 actttaacac acagtaagga ggtttatgat gggaaggtat gaggagctct ttcagaaaag
      841 catcaaagac cccgaagggt tctgggcgca ggcggcggag gcaataacct ggatcacgtc
      901 ctggaacaag gtgctcgacg actcgaacaa gccgttttac cgctggtttt ccggcgccga
      961 gctgaacacc tgcttcaatg ccgtcgaccg gcacgcgatg ttcggcaggg ctgaccaggc
     1021 tgcgatcatc tacgacagcc ccgtcacgaa cacggtccag aaaatcacgt accgggaact
     1081 cctcgacctg gtctcccgct tcgccggagc actggcgaac ctgggtgtac agaagggtga
     1141 caccatcatc atttacatgc cgatgatccc gcaggcggta atcgccatgc tcgcctgcgc
     1201 gcgccttggc gccgtccatt cagtcgtgtt cggcgggttt gcgccccatg agctcgcgat
     1261 caggatcgac gatgcaaaac cgaagatcat catcaccgcc tcgggcgccg ttgaagggaa
     1321 aaaaattctc gaatacaagc ccatgctgga cagggcgatc gaggctgccg cgcacaagcc
     1381 cgagaagaac attatcttcc agcggcagtt cgtgcaggcc tccctgaacc cgcaaacgga
     1441 cctggactgg aatgagctca tggcaaaggc cgcgcccgtc gaatgcactc cggtcaaggc
     1501 caccgatccc ctctacatcc tttacacctc gggcacgacg ggcaaaccga agggcgttgt
     1561 ccgcgacaac ggcggccatg ccgtggccct tcgctggagc atgcgctaca tctacggcgt
     1621 gcaaccgggt gacgtctact gggccgcatc tgacgtgggc tgggtcgtgg gccactccta
     1681 catcgtctac gggccgctcc tccacggatg cacgacagtc ctctacgagg gcaagccggt
     1741 cggcacgccc gatgcgggcg cgttctggcg ggttatctcc cagcacgggg tcaaggtgct
     1801 tttcaccgct cccacggcct tccgcgccat caaacgggag gacccgaaag gggaattcgt
     1861 caaaaagtat gacctctcgc gattcaaagc cctctttctc gcaggtgagc gtctcgatcc
     1921 cgacacctac cactgggccc acaacctctt gcggaagccg gtcatcgacc actggtggca
     1981 gactgaaacg ggctggccca tcaccgcgaa ctgcatgggc atcgaggagt tcccgatcaa
     2041 gcccggctcg tccactaagc ccgttccggg cttcgatgtc acgatcctcg atcccaacgg
     2101 caagaaactc ggccccaagg agacgggctt ggtgaccatc aagcttcccc tccctccggg
     2161 tacgttgccc acgctgtggc aggccgacgc ccggtttgag gagtcctatt tgagagtgtt
     2221 tcccggctac tatttcacga gtgacggagg ctacatcgac gaggacgggt acgtcttcat
     2281 catgggccgg gtggacgacg tgatcaacgt tgccggccac cgcctctcca cgggcgagat
     2341 ggaagaagtc gtctcgacgc acaaggacat cgccgaatgc gcggtcatcg gcgtgcacga
     2401 cgagctcaag ggacaggtcc cgctggggct ttgcgtgctg aagtcgggct cgaacgcgac
     2461 cgagacggcg atcaggaaag atctctccca gatgatccgc gaaaacatcg gccccatcgc
     2521 ctcgtacaag gagacctgcg tggtaaagcg gctgcccaag acccgctcgg gcaagatcct
     2581 gcgcggtacc atgcgaaaga tcgcggatgg agagccgtac accgtgccat ccacgatcga
     2641 cgatccggcg accctgaccg agatcgagga cgcactgaag cacgtggggt acgcgaagaa
     2701 acattgagat ttcaggaagg taggaaggtg agaaggtggg atcagttgcg tttttttctc
     2761 acttcctcac ccgctcacct tcgaagtctt attcacgcat ctgcgttata attgactcat
     2821 cgaataatgc ggcttccggg ccgcattttt cttatgggga gtaaacaatg aaatcagggt
     2881 tcaagctcgg cgactttgaa ctgacctggc tcaacggcgg gaggttcgaa cttgacggcg
     2941 gcgccatgtt cggcgtcgta cccaaggtcc tgtggcagaa gaaatatcct gcaaatgccg
     3001 ataactgcat ccctctccat gcctggccga tcctgatcaa gacgccggac ggcctccttc
     3061 tcatcgaatc cggcctgggc aacaagctta cggacaaaca gaagaaaatc tatcgggtgc
     3121 aggaggagtg gtcgatcgaa gcggacctga aaaagctcgg ccttgcccga gaagacatcg
     3181 actacgtcat cctgacccat tacgacttcg accatgccgg gggcgtggtc atgcaggagc
     3241 cggacgaaag gctctctctc tcatttccga aggcgaaaca tatcatacaa aagaaggaat
     3301 gggaggacgt gctgaacccc aatatccggt ccatcaacac ctactggccg gtgaacaacg
     3361 agcttctgcg ggggagtaag aacctggaac tcgttgacgg cgaggccggg gtcgtttccg
     3421 gcatcagggt cgtccggacc ggcggacata acgagggaca ccagatcgta aagatggaat
     3481 caaaaggcga gcgcgcatac catctcggtg atatcctgcc gacacacgcc catttcaatc
     3541 ccctctgggt catggcctat gacaactacc ctatggattc catagcaatg aaggaaaagt
     3601 ggatcagaag tgcagttgcc gaggaggctt ggttcacctt ctatcatgac gcctttgtgc
     3661 ttgtcgccag atttgacg
//
LOCUS       DASSLD010000177         2866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_311475,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000177 DASSLD010000000
VERSION     DASSLD010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311475"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /gene="tuf"
                     /locus_tag="VFK23_03255"
     CDS             <1..972
                     /gene="tuf"
                     /locus_tag="VFK23_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET7318136.1"
                     /translation="YAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
                     LLARQVGVPYIVVYMNKVDMVDDPELIELVELEIRELLSKYQFPGDKTPIVKGSALKA
                     LQSESKDPNAPEYKSILELMVAIDSYIPEPKRDIDKPYLMPIEDVFSIAGRGTVVTGR
                     IERGIVRVGDEIEIVGIKPTQKTVVTGVEMFRKLLDQGQAGDNVGVLLRGIKKEEVER
                     GMVLAKPGSITPHTKFKAEAYILTKEEGGRHTPFFNGYRPQFYFRTTDVTGVVKLAEG
                     VEMVMPGDNMTCSVELITPIAMDQGLRFAIREGGRTVGAGVITQVIE"
     gene            1088..1240
                     /gene="rpmG"
                     /locus_tag="VFK23_03260"
     CDS             1088..1240
                     /gene="rpmG"
                     /locus_tag="VFK23_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET7318137.1"
                     /translation="MREIVLLACSECKRKNYSTTKNKRNTPDKLNLDKYCRFCRKHTV
                     HKETKA"
     gene            1278..1353
                     /locus_tag="VFK23_03265"
     tRNA            1278..1353
                     /locus_tag="VFK23_03265"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1311..1313,aa:Trp,seq:cca)
     gene            1389..1574
                     /gene="secE"
                     /locus_tag="VFK23_03270"
     CDS             1389..1574
                     /gene="secE"
                     /locus_tag="VFK23_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET7318138.1"
                     /translation="MFKRLMEFLQEVKLELKKVVFPTKKEVIGSTWVVITTVLICAFF
                     LGLVDLGLGRLMTLAFK"
     gene            1583..2128
                     /gene="nusG"
                     /locus_tag="VFK23_03275"
     CDS             1583..2128
                     /gene="nusG"
                     /locus_tag="VFK23_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET7318139.1"
                     /translation="MPMNWYVVHTYAGFENKVKTSILETVSKLELQHKIGQILVPTEE
                     VLSIKAGKKRKSQRKFFPGYILVEMEMDDNTWHIIKETPKVTGFVGGSTNPTPLSEDE
                     VKTILKQAEEGAVAPTQPLHAYQKGDRVRVIDGPFSSFIGTVEDVNPAHGRLRVMVSI
                     FGRSTPVELEFLQVEREKESK"
     gene            2184..2609
                     /gene="rplK"
                     /locus_tag="VFK23_03280"
     CDS             2184..2609
                     /gene="rplK"
                     /locus_tag="VFK23_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET7318140.1"
                     /translation="MAKEITAKIKLQVPAGKANPAPPVGPALGQHGVNIMEFCKTFNA
                     RTQKMGETIVPVIITVYSDRSFTFITKTAPAAELLKKAAGIVKGSGVPNKDKVGKVTR
                     AQIREIAQTKLPDLNAATVESAMRMIEGSAKSMGLEIAD"
     gene            2742..>2866
                     /locus_tag="VFK23_03285"
     CDS             2742..>2866
                     /locus_tag="VFK23_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET7318141.1"
                     /translation="MAGKKYKAVAALVDRAKKYGLEEAIGLAKKTARTKFDETVDL"
BASE COUNT          728 a          729 c          878 g          531 t
ORIGIN      
        1 tatgcccatg tcgactgccc cggccatgcc gactacgtca agaacatgat caccggggcc
       61 gcccagatgg acggcgccat cctggtggtc tcggccgcgg acggccccat gccccagacc
      121 cgggagcaca ttctgcttgc gcgtcaggtg ggggtgccct acatcgttgt gtacatgaac
      181 aaagtggaca tggtggacga ccccgagctg atcgagctgg tggagctcga gatccgggag
      241 cttctgtcca agtaccagtt cccgggcgac aagacgccga tcgtgaaggg gagcgcgctg
      301 aaggccctcc agagcgagag caaggacccg aacgcgccgg aatacaagag cattttagag
      361 ctcatggtgg ccatcgacag ctacattccg gagcccaaac gcgacatcga caagccgtac
      421 ctgatgccga tcgaggatgt gttttcgatt gccggccgcg gcacggtggt gacgggaagg
      481 atcgagcgtg gcatcgtgag ggtgggcgac gaaatcgaga ttgtggggat caagccgacg
      541 cagaagacgg tggtgacggg cgtcgagatg ttccggaagc tccttgatca gggccaggca
      601 ggcgataacg tgggggttct tctccggggc atcaagaagg aagaggtgga acgcgggatg
      661 gtattggcca agcccgggag catcacgccg cacacgaagt tcaaggcgga ggcgtacatt
      721 ttgacgaagg aagagggcgg ccgccacacg ccgttcttca acggttaccg tccgcagttc
      781 tacttccgga cgacggacgt gacgggagtg gtgaagctcg ccgagggcgt cgagatggtg
      841 atgccgggcg acaatatgac gtgcagcgtg gagttgatca cgccgattgc gatggaccag
      901 gggcttcgct tcgccatccg tgagggcggc aggaccgtgg gcgccggcgt tatcacccag
      961 gtgatcgagt agcgtaaaac gagggacgat agacggggga cgaaacagcg tgcgtccgtc
     1021 gtccgagcga gcgaagcgag tggtcgccca tcgttcgtcg taatttcaga agagtgaggc
     1081 catacaaatg cgggaaatag tacttctcgc ttgcagtgag tgcaagcgca aaaactattc
     1141 aacgaccaag aataaaagaa acacaccaga caagttgaac ctggacaagt attgccggtt
     1201 ctgtcgcaaa cacaccgtgc acaaagaaac caaggcatag ctctagtaaa cgcatggcgc
     1261 cgcggggttt aggacacagg ccagtagctc taatggtaga gctccggact ccaaatccgg
     1321 ctgctggggg ttcgagtccc tcctggcctg ccattttttt agagccctgt gcggagaaag
     1381 gcccggatat gttcaaaagg ttgatggaat tccttcaaga ggttaagctg gagctcaaga
     1441 aagttgtgtt tccgacaaaa aaggaggtca taggttctac gtgggtggtg atcaccaccg
     1501 tgctcatatg cgcctttttt ctggggctgg tagatttggg gctgggaaga ttgatgaccc
     1561 tcgcctttaa gtaaggatca ttatgccgat gaactggtat gtcgttcata catatgcagg
     1621 ttttgagaat aaggtaaaga ccagcattct ggaaaccgtt tccaaactcg agcttcagca
     1681 caagatcggc cagatcctcg tcccgaccga ggaggtgctg tccatcaagg ccggaaagaa
     1741 gcgcaaatcg caacggaaat ttttccccgg atatattctc gtcgagatgg agatggacga
     1801 caacacctgg catatcatca aggaaacgcc caaggtgacc ggctttgtcg gcggcagtac
     1861 gaaccctacg ccgctctccg aggacgaggt gaaaaccatt ctcaagcagg ccgaagaagg
     1921 cgcggtagcc cccactcagc cgttgcatgc ataccagaag ggggaccggg tcagggttat
     1981 cgacgggcct ttcagcagtt ttatcggcac ggtcgaagac gtcaatcccg ctcacggaag
     2041 actgcgggta atggtgagca tcttcgggcg ttcgacgccg gttgagctgg aatttcttca
     2101 ggtcgagcgc gaaaaggaat ccaaataatc aattcaggat actcgattca aaatagagtc
     2161 gaaatacgga caggagcatt tagatggcta aagagatcac ggcaaaaatc aaacttcaag
     2221 tccccgcagg aaaggctaat ccggccccgc cggtgggtcc cgcgctcggc cagcacggag
     2281 tgaatatcat ggagttctgc aagacattca acgccaggac ccagaagatg ggcgagacga
     2341 tcgttcccgt cattataacg gtatattcgg acaggtcgtt cacgttcatc acgaagacag
     2401 cgccggctgc agagctgctg aaaaaagccg cgggtatcgt caagggctcc ggcgtgccga
     2461 ataaggacaa ggtcggcaag gtgacccgcg cccaaatcag ggaaatcgcc cagaccaagc
     2521 tcccggacct caatgccgca accgtcgaat cagccatgcg catgatcgag ggctcggcca
     2581 agagcatggg gcttgagatc gccgattaac gacagactac gaaggttgaa ttgaaaattg
     2641 gatgttggaa tttaccattc tcaatatgca atcttcaatt ttgagtaaag aatttacatg
     2701 cgggaggctg acaacagccg ctaccaccgc gaggagggac catggcaggg aagaagtata
     2761 aagcagtggc ggcgctcgtg gaccgggcaa aaaagtacgg cctcgaggag gccatcggcc
     2821 tcgccaagaa aacggccagg accaaattcg atgagaccgt cgacct
//
LOCUS       DASSLD010000178         2435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_278926,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000178 DASSLD010000000
VERSION     DASSLD010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278926"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            415..765
                     /locus_tag="VFK23_03290"
     CDS             415..765
                     /locus_tag="VFK23_03290"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HET7318142.1"
                     /translation="MQVNLRVIALLFLILLSCCAAKRSGPEVSNGVVRFEFIAPGASS
                     VAVAGSFNRWDPRKNPLSGPDSKGVWSVTLELPAGRYEYLFVVDGNKWLPDPGTPSVD
                     DGLGGRNSIVEVGR"
     gene            899..1462
                     /locus_tag="VFK23_03295"
     CDS             899..1462
                     /locus_tag="VFK23_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7318143.1"
                     /translation="MDERTTSDEYDLIRRCLAGDTDRFSVLVDRYKSMMYSIAYRMLG
                     DQDAANDMAQESFIAAFTGLGNFQFGSKFSTWLYQILMNKCRDYLRAGKNKVSVDDIA
                     ELRSDPGDTPEQAAQAHELGDAVQKALDGLPPDYREVIVLKHIEGLDYDEISKIVGAS
                     VAALKVRAHRGREMLKDILERVGIRNE"
     gene            1455..2114
                     /locus_tag="VFK23_03300"
     CDS             1455..2114
                     /locus_tag="VFK23_03300"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318144.1"
                     /translation="MNKDIEIIHKYFDGDMTAAEKEAFLRRLSSEPELKKEFDALSRT
                     LRIVAESPRPAVPLSFTGDVMNRLRPAPVPWGKRIRSFLFASRTLRWNMATAFASALV
                     LIVSLLTVSRQYAPPTGPTGAAFRQTAQESAVTVRLNLYAPQAKNVAVSGDFNKWRVD
                     SDVMSRQEGGVWTIDIKLNPGVYTYMFVVDGKTWVTDPDAETYQDDGFGNKNAVMRVR
                     T"
     gene            2111..>2435
                     /locus_tag="VFK23_03305"
     CDS             2111..>2435
                     /locus_tag="VFK23_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318145.1"
                     /translation="MKTVRGLRESFFVIIILSSFVMIMGLGGAAASGQEVLPEKIKAA
                     VERSPLSGQGKAEILDAAGRALRAGIPADDVEVIISRGRDRGVGAGTTRELIDTAVKA
                     REKGLP"
BASE COUNT          512 a          678 c          747 g          498 t
ORIGIN      
        1 cgccgttttc ctctttttct cgacgagctc gttcgggccg tagcgcacca gccgctccag
       61 cgcttcttcg gcggtgaggc cgtcgcgtcc cgaattgagt tcggcaagcg catctttgac
      121 ggtcatctgg tgaaattgca aaggaccctc acttattctg tcacgcctgc tgcgaaactt
      181 gtcctcgaat gatttaatcg ggagcaggca tccctggtct cacaaaatgg attcctaccg
      241 gggcctgccg cgcatgattc cggccggggc ggaatgacaa aaagatttag taccaaaaag
      301 gtagcatggt ttcgtttgct tgtaaagctg cgaggtcatc gtccaccctg cgtgatatac
      361 ttaaataagt ttctttttgt cctgtacccg ttttcccgca aaggcagatc aatcatgcag
      421 gtcaacttgc gcgtcatagc actgctgttt cttatcctgc tgtcctgctg cgctgcgaag
      481 cggtcaggac cggaagtgag caatggcgtt gtgcggtttg aattcatcgc gcccggtgcc
      541 tccagcgtcg ccgttgccgg cagtttcaac cggtgggacc cgcgcaagaa tccgctttcc
      601 ggcccggaca gcaaaggcgt atggtccgtc accctggaac tgcccgcggg acggtacgaa
      661 tacctcttcg tagtggacgg aaacaagtgg ctgccggacc ccggcactcc ctcggtcgac
      721 gacgggctgg gggggagaaa ctctatcgtt gaagtaggaa gatgacggcc tgtaaccttt
      781 atgcccttca atcgtctcat agttaaaaag aggaaccagc ttcccgtttc cggcggaagc
      841 acggataagt cattgagagg acaggggctt cagccccctc atctcccggg agtccggcat
      901 ggatgaacgg acgacatcgg atgaatatga ccttatccgg aggtgcctcg caggcgatac
      961 ggacaggttt tccgtactgg tggataggta taagtcgatg atgtattcca tcgcatacag
     1021 gatgctcgga gatcaggacg cggccaacga tatggcgcag gagagtttca tcgccgcctt
     1081 tacgggcctc gggaattttc aattcggttc caaattttcc acgtggctgt accagatcct
     1141 gatgaacaag tgccgggact acctccgggc cgggaaaaac aaggtttccg tcgatgacat
     1201 cgcggaactc cgctcggacc ccggggacac gcccgagcag gccgcgcagg cgcatgagct
     1261 cggcgacgcg gtccagaaag cgctcgacgg attgccgccg gactatcgcg aggtgatcgt
     1321 gctcaagcac atcgaagggc tggattacga cgagatttcg aagatcgtgg gagcgagcgt
     1381 cgcggcactc aaggtgaggg cgcaccgggg cagggaaatg ctcaaagata tcctcgaacg
     1441 ggtaggaata aggaatgaat aaggacattg aaataatcca taagtatttc gacggcgaca
     1501 tgaccgcggc ggagaaagag gcctttctca gacgcctttc ctccgagccg gagctcaaaa
     1561 aagagttcga cgccctttcc cggacgcttc gcatcgttgc ggagagcccg cgacccgccg
     1621 ttcccctttc gtttaccgga gacgtcatga accggctgcg gcccgctcct gtcccctggg
     1681 gcaagcggat caggtcgttc ctgttcgcaa gcaggacgct tcgctggaat atggccaccg
     1741 cctttgcgtc ggcgctcgtt ctgatcgtca gccttctcac ggtttcccgg cagtatgctc
     1801 ctccgacggg cccgacgggc gccgcgttcc gtcagaccgc gcaggagtcc gcagtaaccg
     1861 tgcgcctgaa cctgtatgca cctcaggcga agaacgtcgc cgtgtccggc gatttcaaca
     1921 aatggcgggt cgacagcgat gtgatgagca ggcaggaggg cggggtctgg accatagaca
     1981 tcaagctgaa cccgggggtc tatacctata tgttcgtggt cgacggcaag acatgggtca
     2041 cggaccctga tgcggaaacg taccaggacg acggcttcgg gaacaagaac gccgtgatga
     2101 gggtaaggac atgaaaaccg tcagaggatt gagggaaagt ttttttgtta tcatcatcct
     2161 gtcgtcgttc gtcatgatca tgggcctggg gggagcggcc gcttccgggc aggaggtgct
     2221 gcccgaaaaa atcaaagcag ccgtcgagcg ttcaccgctt tcgggccagg ggaaagcgga
     2281 gattctcgac gcggcgggcc gcgctctccg ggccggcatc cctgccgacg atgtggaggt
     2341 tatcatatcc cggggacggg accggggggt cggcgccggt acaacccggg aactgatcga
     2401 taccgcggtc aaggctcggg agaagggcct gccgg
//
LOCUS       DASSLD010000179         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_350208,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000179 DASSLD010000000
VERSION     DASSLD010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350208"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..703
                     /locus_tag="VFK23_03310"
     CDS             32..703
                     /locus_tag="VFK23_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7318146.1"
                     /translation="MSNKAARNLFIIGSLIFFAIFLALTFDTLGQLDKRAPEITADVD
                     AGKLIWQKYDCIGCHTIFGNGSYFAPDMTKVVDRKPKGYLKKFIMDPKAVKATAAMPT
                     LGITSDEADKLIAFFEWVDKVDTNGWPPKPVLAAAAGVGGQELSPGQRVYRAQGCSAC
                     HAINGIGGTSGPELTKVGTRHDRAWFIGHFKDPDDFVKNSAMPKVTAPDEEIAQLADY
                     MLTLK"
     gene            786..2171
                     /locus_tag="VFK23_03315"
     CDS             786..2171
                     /locus_tag="VFK23_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET7318147.1"
                     /translation="MKYQSQKIAQYFYTFAILLFLVQVIVGIVAAIQFIWPDFFILKF
                     NIIRTLHINALVVGLLAGLMGATYYVVPEESETELWSYPLALFQFWATVVAVTAVVLG
                     YIWMGIDPQANKVIFGTPVLNEGREYIEAPRWADVLIVVSVLLFLLNNFMTMLKTKKW
                     TGIQGVLLGGLLFLALMYLPGMFYTKSMVKDQFWWWWVIHLWVEGAWEVIAGALLAFM
                     LMKVIGAKREVLEKWMYIEVGLVMFTGILGTGHHYYWIGTPKYWLWVGGIFSSLEPVP
                     LLLMVWDAFRTTREARNVTNRAGLYYTVAHAIFNFVGAGLWGVIHTLPQVNQWTHGTQ
                     ITTAHGHLAFYGAYVLLVMSMIYVTLPALRGVKEYDPGRAFASFWWMTISMVFIVLTI
                     TGAGMVQTYMERLMGLDYVAVKANYNLWFWILRAIFGVGFLVGVAIFVVDYFTLGKKP
                     YPALQEARERG"
     gene            2345..>2724
                     /locus_tag="VFK23_03320"
     CDS             2345..>2724
                     /locus_tag="VFK23_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7318148.1"
                     /translation="MKKILLIDDDAIFLRLLQEYVEERYPGLRIMTCNDSVKCLADIT
                     NDLDLLLVDLEMPGLDGSKVLSYAVSKGVSKNRIIILSARDAEYLHERFPMGSCLAVL
                     NKFEVQQQGVLDMIFKSLQEKSAG"
BASE COUNT          575 a          780 c          793 g          576 t
ORIGIN      
        1 ctttgtgaat cttattcaag gaggtgcata catgagcaac aaggcagcaa ggaatctctt
       61 tatcatcggc agtctcattt ttttcgcgat attccttgcc ctcacgttcg acacgctcgg
      121 acagctcgac aagagggcac cggaaattac cgcggatgtg gacgccggta agctgatctg
      181 gcagaagtac gattgcatag gatgccacac cattttcgga aacggttcgt acttcgcgcc
      241 ggacatgacc aaggtcgtgg atcggaagcc caagggatat ctcaagaagt tcatcatgga
      301 tcccaaggca gtgaaggcga cggcagccat gcccacgctc ggcatcacgt cggatgaagc
      361 agacaagctg atcgcgttct tcgagtgggt cgacaaagtc gacacgaacg gctggccgcc
      421 caagcccgtc ctggccgccg cggccggagt cggaggacag gaactctctc ccggccagcg
      481 cgtataccgg gcccagggct gctccgcctg ccatgcgatc aacggcatag gcggcacatc
      541 gggccccgag cttaccaagg tcggaaccag gcatgaccgg gcatggttca tcggacactt
      601 caaggacccc gacgattttg tgaagaattc ggccatgccg aaagtcacgg cgcccgacga
      661 ggagatcgcc caactggccg attacatgct gacgttgaag taattcccgg cacgacattc
      721 caacgattcg atccccgaca gattcgtcag gggatgacaa tacaagggag ggtggcacaa
      781 tgaccatgaa ataccaaagc caaaagatcg cgcagtactt ttacaccttc gcgatcctgc
      841 ttttcctggt ccaggtgatc gtcggcatcg ttgcggcaat ccagttcatc tggccggact
      901 tttttatcct gaagttcaat atcatcagaa cgctccatat caacgcgctc gtggtagggc
      961 tccttgccgg cctcatgggg gcgacgtatt acgtcgtgcc cgaggaatcg gagacggagc
     1021 tgtggagcta tcccctggca ctgttccagt tctgggcaac ggtcgtggcc gtcacggctg
     1081 tcgtgctggg ctacatctgg atgggcatcg acccccaggc gaacaaggtg atattcggca
     1141 cccccgtcct gaacgagggc cgggaataca tcgaggcgcc gcgctgggcg gacgtgctga
     1201 tcgtcgtctc ggtgcttttg ttcctgctca acaatttcat gaccatgctg aagacgaaga
     1261 agtggaccgg catacagggc gttcttttgg gcggcctcct cttcctcgcg ctcatgtacc
     1321 tgccgggcat gttctacacc aagagcatgg tcaaggacca gttctggtgg tggtgggtga
     1381 tccatctctg ggtcgaaggg gcctgggagg tgatcgccgg ggcgctcctc gcgttcatgc
     1441 tcatgaaggt gatcggcgcg aagcgtgagg tgctggaaaa gtggatgtac atcgaagtcg
     1501 gactcgtgat gttcaccggc atcctcggta cgggccatca ctactactgg atcggcacac
     1561 ccaagtactg gctttgggtc ggcggcattt tcagctccct ggaaccggtc ccgctgctcc
     1621 tcatggtttg ggacgccttc cgcaccaccc gtgaagcccg gaacgtgacg aaccgcgcgg
     1681 gcctctatta caccgtggcc cacgccatct tcaacttcgt cggcgccgga ctctggggcg
     1741 tgatccatac ccttccccag gtaaaccaat ggacccacgg cacgcagatc accacggccc
     1801 acgggcacct tgcattctac ggggcgtatg ttctgctcgt gatgtccatg atctacgtga
     1861 ccctgccggc cttgaggggc gtcaaagaat acgatccggg aagggccttc gcgtccttct
     1921 ggtggatgac catatcgatg gtcttcatcg tgctgaccat aaccggtgcg ggcatggttc
     1981 agacctacat ggagcgcctc atgggcctcg actacgtggc cgtcaaggcg aactacaacc
     2041 tctggttctg gatcctccgc gcgatattcg gcgtcggctt cctcgtcgga gtcgcgatct
     2101 tcgtggtgga ctatttcacg ctgggaaaga aaccgtaccc agcgctgcag gaggcgcggg
     2161 agcgcggata gcaaccgccg tttcatcgtt tacgtcaaag gggaccgcac attgcggtcc
     2221 cccttttttt gatggcggaa agccatatgc tttgcatcat cgcgtgaatt ctggtaaagt
     2281 acaggaaaag cgaattacgg accggcgagc gtcgtcgagt gctcgttcgg agccatgggg
     2341 aacgatgaaa aagatcctgc ttatcgatga cgatgccata tttctgaggt tgctccagga
     2401 gtacgtcgag gaacgttatc ccggtctccg gatcatgacc tgtaacgact cggtaaaatg
     2461 cctcgccgac atcacgaatg acctcgacct cctcctggtc gatctcgaga tgcccggcct
     2521 ggacgggtcc aaggtgctct cctatgccgt gtcgaagggc gtaagcaaga accggatcat
     2581 catcctgtcg gcgcgcgacg cggagtacct gcacgagcgt ttccccatgg gaagctgcct
     2641 cgccgtgctc aacaaattcg aggtccagca acagggcgta ttggacatga tcttcaagtc
     2701 tttgcaggaa aaaagtgcgg gaaa
//
LOCUS       DASSLD010000180         8965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_50188,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000180 DASSLD010000000
VERSION     DASSLD010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 8965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8965
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50188"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFK23_03325"
     CDS             <1..508
                     /locus_tag="VFK23_03325"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HET7318149.1"
                     /translation="PKKVIDLQQQMLLGVLIIILLAMGMWFFWNRQANKIATLQKEKI
                     AAEARVRDQENVLKEVKNVEEERKQYKEKIGIIQQLKKNQSGPVRLLDEISKALPPGV
                     NVVSITESNNNINIEGQAFTNNDVVRFVDNLKASSLLTDVTLLETSQAKLEGIDVYKY
                     KLQFVYKG"
     gene            511..1125
                     /gene="pilO"
                     /locus_tag="VFK23_03330"
     CDS             511..1125
                     /gene="pilO"
                     /locus_tag="VFK23_03330"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HET7318150.1"
                     /translation="MAAILDKIQKISPRTRLIVFLALIVLLLVAYILQFQIPMNTEIK
                     NLEQALSGLNAKIQDNDSKIRNLEALKSEVKILQTRLAELTEQLPPESEVSGLLRQIQ
                     NLVNQSGLVLKLWRPEKRRTHASGLYEEIPITLSLAGGYHNTAILFDRIRTLPRIVNV
                     LNLKMGGAKVAKDGSVNIEINCTAMTFAAVEKKGEATTSGNKTQ"
     gene            1163..1645
                     /locus_tag="VFK23_03335"
     CDS             1163..1645
                     /locus_tag="VFK23_03335"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HET7318151.1"
                     /translation="MSAWGCSGSNQAPATAPKPIAAKPVQKPISLPTPTVAEEKAQAP
                     YSYNAQGRRDPFASIVVKEEKLAKLGNRPPLERFNISEFKLTGVVWGGFGYNAMMEGP
                     DGKGYFVRVGTVIGLNKGIVKKITQDRMVIEEKFKNFSGETERKEIVVELRKKQEEMQ
                     "
     gene            1642..3954
                     /gene="pilQ"
                     /locus_tag="VFK23_03340"
     CDS             1642..3954
                     /gene="pilQ"
                     /locus_tag="VFK23_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HET7318152.1"
                     /translation="MTLTQPPKAYWLIFLIFLASMLHGCATTAPEPEKQGPQVVLTKI
                     EPREVDGKTEIAIEGTAPILQYTSFQLTEPLRLVIDIADADIGTLRDRLAVNKGAVTD
                     ITPSQKDNIARLEIGLAQAVESKVYQSDGSLVVEFVKPVEEAKTTPETASAVTETTPP
                     VSEQAPPPAAEKKETQQPERAAATVVTSVRASAGKAGVKVVITADGAMSPKAFMVSGN
                     KLVVDIPGTKSRVRPSVIPVRKGGLDKVRVGQHAAPDQKVRVVMDLTKTMQYTVTPDE
                     NTVLIAMSESSPAVTREQQPQERAAAQEQEKGAPVEKEVAAPQPPQQAVETAAAVPPV
                     KEQQTEAAVATGTGKFTGRKISLDLQDADLVNVMRLFAEVANLNVIVSPDVRGKVTVR
                     MVNIPWDQAMDIILKMNGLGYALEDNILRIASVSALTKEAEEELRSKEAKKKAEDLIT
                     RMVPINYATASAIEGTIKKSLSPRGETVTDTRTNTLIIKDIPRNVDEVVALIKLLDKP
                     IPQIMIEARIVEASLTFSRQLGVQWGGNFKADAAHGNPTGVTFPNSIGVTGGPTMGSV
                     PSGNGNFVVNMPAAAGAGTGGGAIGFTFGSLSKALNLDLVLSALESTGEGKIISSPRV
                     SALDNKEAKIEQGVSIPFSTTSAGGTQIQFIDAKLALTVTPHATPDKKIFMKITATKN
                     APDTSILGASGQPSIRKNEATTEILLADGETAVIGGILTTNKTVAYTKVPFFGDIPLI
                     GWLFKTKTTSENKGELLIFITPRVVKQEVI"
     gene            complement(4661..4813)
                     /gene="rpsU"
                     /locus_tag="VFK23_03345"
                     /pseudo
     CDS             complement(4661..4813)
                     /gene="rpsU"
                     /locus_tag="VFK23_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
     gene            complement(4824..6185)
                     /gene="der"
                     /locus_tag="VFK23_03350"
     CDS             complement(4824..6185)
                     /gene="der"
                     /locus_tag="VFK23_03350"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET7318153.1"
                     /translation="MQKPIIAIVGRPNVGKSTLFNRILGRNLAIVEDMPGVTRDRNYA
                     EAEWEGKRFLLVDTGGFEPETEDPMYLKMREQTTLAVEEADIIIFLMDGRQGLLPSDI
                     EVSHRLRASGKPVIYAVNKVDGARHEELIPDFYRLGIDAFFPLSALHGVGFSDLMDEL
                     WRMLPATPAGREDEEQVSIPRVAILGRPNVGKSSFVNSLIGEDRMIVSPTSGTTRDAV
                     DSEYRYYGRKYVLVDTAGIRSRGRIGQGVEKYSVMRAMKGLSRADVALVLIDAAEGVT
                     EQDERIAGLVNEAGKGLILVLNKWDLVPDKELAYKKFIADIQQRLKFAHYAPVLTISA
                     VTRQRITKVFDEIDAVIKEAEKRVPTAELNRVFEKLTAAHEPPLYRGKRVKYYYLTQV
                     AVKPPTFIAFVNYPEGVHFSYLRYIENGLRDAFGFRGTPLRLFAKRRREERSAEKNKV
                     LKS"
     gene            6507..7031
                     /locus_tag="VFK23_03355"
     CDS             6507..7031
                     /locus_tag="VFK23_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HET7318154.1"
                     /translation="MLRCVVLAACVFFIAATSAHAEFYRWVDKDGKEFFTNELEKVPQ
                     EYRESVQTVKPDESRVSVGDRPSAVVKPGGLSGEHKDKNGRGEEYWRKKAANLRLKLR
                     DQQDEYNFILKKLENQDQNPNQLAGKKSRSVSSLEKKKLKLEKEIAQTRRKLEVDLPE
                     EARRADAYPGWIRE"
     gene            7083..>8965
                     /gene="leuS"
                     /locus_tag="VFK23_03360"
     CDS             7083..>8965
                     /gene="leuS"
                     /locus_tag="VFK23_03360"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET7318155.1"
                     /translation="MDERYDFKKVEERWQRSWEEHRTFKVIEDASKPKYYCLEMFPYP
                     SGRIHMGHVRNYAIGDVVSRYKRMRGFNVLHPMGWDSFGLPAENAAIKHNIPPSKWTL
                     ENIAFMRDKQLKRLGLSYDWDREVTTCLEEYYRWNQWFFIRMFERGLAYKKLSYVNWC
                     PSCSTVLANEQVENGQCWRCGSLVTHKELDQWFFKITDYAEELLAHCDRLTGWPERVL
                     TMQRNWIGKSVGVEVDFAIEGTGEKLTIFTTRQDTLFGVTFMSIAPEHPMLESLIKGR
                     SRADDVRAFVQRVLREDKAVRTDEKQEKEGIFTGAYAINPMNGSKVPVWVGNFVLMEY
                     GTGAIMSVPAHDQRDFEFAKKYGLPVQVVIQSPGKDLMADTMKQAYVEDGIMANSGVF
                     DGLTNTAAKEKIADYIEQSGLGKRTVNWRLRDWGISRQRYWGTPIPIVYCDACGTVPV
                     PDKDLPVRLPVDVELTGKGRSPLAESKSFRETVCPKCGGKARRETDTMDTFVDSSWYF
                     LRYCSPRENKAPFDKKAAGYWMSVDQYIGGIEHAVLHLLYARFFTKVVRDLGLFDGDE
                     PFKNLLTQGMVIKDGAKMSKSKGNVVDPDHILAKYGADTARLFSLFAAPPERDLDWSD
                     QGV"
BASE COUNT         2237 a         2335 c         2508 g         1885 t
ORIGIN      
        1 tccaaagaag gtaatcgatc ttcaacagca gatgcttctc ggagttttga tcattatcct
       61 ccttgcgatg gggatgtggt ttttctggaa cagacaggca aacaagatcg caacccttca
      121 aaaggaaaag attgcagcag aggcgcgtgt gcgggatcag gagaacgtcc tgaaggaagt
      181 gaaaaacgta gaggaagaac gaaaacaata caaagaaaag atcgggatca ttcagcaact
      241 caagaaaaat cagtcgggac ctgtccggct gctcgacgaa atcagcaagg cgcttcctcc
      301 cggtgtgaat gttgtgtcca ttaccgagag caacaacaat atcaacatcg aaggccaagc
      361 cttcacgaac aacgatgtcg tccgttttgt cgataatctg aaggcatcca gtctattgac
      421 ggacgttacg ctattggaaa cgagtcaggc caaactcgag ggaatagacg tgtacaaata
      481 caaactccag ttcgtctata agggatagcg atggctgcga tattagacaa aatacagaaa
      541 atctcgccca ggacacggct gatcgtgttt ctcgccctta tcgtactgct cctcgtggcg
      601 tacatacttc aattccagat acccatgaat acggagatca agaatctgga gcaggcgctt
      661 tcaggcctga atgcgaagat tcaagacaat gacagcaaga tcagaaatct tgaagcattg
      721 aagagcgaag tcaagattct tcagacgcgt ctcgccgaac tcaccgaaca gctcccgccg
      781 gagtccgaag tgtcgggtct gctcaggcag atacaaaacc tcgtgaatca gtccggtctc
      841 gttctcaaac tctggaggcc tgagaaacga agaactcatg cgagcggtct gtatgaggaa
      901 atccccatta ccctctcgtt ggccggcggg taccataata ctgccatttt gttcgatcgc
      961 atcagaacat tgccccgtat cgtcaacgtc ctgaatttaa aaatgggagg agcgaaagta
     1021 gcaaaagacg ggagtgtcaa tatcgaaatc aattgtaccg cgatgacttt cgcggcggtg
     1081 gagaaaaaag gtgaagccac aacatccggg aataaaacgc agtaaataca ctcttccctt
     1141 gacgctgttc ctgttgcttg gcatgtccgc atggggatgc tccggctcga accaggcgcc
     1201 ggccacggcg cccaagccga ttgccgcgaa gccggtccag aagccgataa gtctgcccac
     1261 gccgacagtc gccgaggaga aggcgcaggc tccctattcc tacaatgcac agggaagaag
     1321 ggacccgttt gcctctatcg tcgtgaagga agaaaaactg gcaaaattgg gaaatcgtcc
     1381 gccgcttgaa cgattcaata tctcggagtt caagttgaca ggggtcgtct ggggcggctt
     1441 cggatataac gcgatgatgg aaggccccga cggcaaggga tactttgtac gcgtcggcac
     1501 cgtaatcggg ctgaacaaag gtatcgtaaa gaagattacg caagacagga tggtcattga
     1561 agagaaattc aagaattttt ccggtgaaac agagcgcaag gaaatcgttg ttgagctccg
     1621 gaaaaaacag gaggagatgc aatgacactt acccaaccgc cgaaggcgta ttggttaata
     1681 tttctcatct ttctcgcatc aatgctccat gggtgtgcga cgacggcgcc ggaacccgag
     1741 aagcaggggc cccaagtcgt tcttacgaaa attgagcccc gggaagtgga cgggaaaacg
     1801 gaaatagcca ttgaaggaac cgcgcccata ttgcaatata cgtcattcca actgaccgaa
     1861 cctctgcggc tggtcatcga catagccgat gccgacattg gcacattgcg ggaccgattg
     1921 gccgtcaaca agggagcagt caccgacatc acgccgagcc agaaagacaa tatcgcccgg
     1981 ctggagatcg gactcgcgca ggccgtagaa tcgaaggtgt atcaatccga cggtagcctt
     2041 gtcgtggaat tcgtcaaacc tgtagaagag gcaaagacaa cacctgagac agcgtcggct
     2101 gttacagaaa cgacgccgcc ggtctcagaa caggcccctc cgccggcagc ggaaaaaaag
     2161 gaaacccagc agcccgaaag ggccgcggca acggtcgtaa cgtcagtccg cgcctcagcc
     2221 ggcaaggcgg gggtgaaggt ggttatcacg gccgatgggg ccatgtcgcc gaaagccttc
     2281 atggtttccg ggaacaagct tgtcgtcgat atcccgggta cgaaaagccg tgtacggcca
     2341 agcgtcattc ccgtcagaaa gggcgggctc gacaaagtcc gtgttgggca gcacgccgcg
     2401 ccggatcaaa aggtgcgcgt ggtgatggac ctgaccaaga cgatgcagta cacggttaca
     2461 cctgatgaaa ataccgtgct tatagcaatg agcgagtctt ctcccgccgt gacccgcgaa
     2521 caacagcctc aggagagggc tgcggcgcag gagcaggaaa agggcgcgcc cgtggaaaag
     2581 gaggttgccg ctccccaacc gccccagcaa gcagtggaaa ccgcggcagc ggtccctccg
     2641 gtcaaggaac aacagaccga ggccgctgtg gcgaccggaa cgggcaagtt cacgggacga
     2701 aaaatctcgc tcgacctcca ggatgcggat ctggtgaatg tcatgcggct ttttgccgag
     2761 gttgcgaatc tgaatgtcat tgtctcacct gatgtccggg gaaaagtgac tgtgcgtatg
     2821 gtcaatattc cctgggatca ggcaatggac attatcctca aaatgaacgg tttggggtac
     2881 gccttggagg acaatatcct gcgcattgcc tccgtatctg ccttgacaaa ggaggctgag
     2941 gaagagttaa ggtcgaagga ggccaagaaa aaggcagaag atctaatcac gcgcatggtc
     3001 ccgataaatt atgcgacggc ttctgcaatt gagggtacga tcaagaagtc gctctctccc
     3061 cgcggtgaga ccgtaacgga tacgaggacc aacaccctca ttatcaagga tattccacgg
     3121 aatgttgatg aggttgtggc tctcatcaag cttctcgata aaccgatacc tcagatcatg
     3181 atcgaagcga ggatcgttga ggcgtcgctc actttcagcc gccaactcgg tgtgcagtgg
     3241 ggcggaaact tcaaggccga cgccgctcac ggaaacccga caggtgtaac cttccccaat
     3301 tcaataggcg tgaccggcgg accaactatg ggatcggtcc cttcgggaaa tggcaacttt
     3361 gtggtaaata tgccggcggc cgcaggagcc ggaaccggcg gcggtgctat cggcttcacc
     3421 ttcggttccc tcagcaaagc gcttaatctg gacctcgtcc tttcagcctt ggagtccacg
     3481 ggcgaaggca agatcatatc atcaccacgc gtcagcgcgc ttgacaataa ggaagccaag
     3541 atcgagcagg gcgtctctat tccgttctca accacgtccg cgggggggac gcagatacag
     3601 tttattgatg ccaagctggc gcttacggtc acgccgcacg caacgccgga caagaagata
     3661 ttcatgaaga ttactgctac gaaaaatgcg cccgatacct ccattctggg cgcgtcgggt
     3721 cagccttcca tacgaaaaaa tgaggcaaca acggagattc ttctggccga cggagagacg
     3781 gctgtgatcg gtggcatcct cacgaccaac aagaccgttg catacaccaa ggtcccgttt
     3841 ttcggcgata ttccccttat cggctggctc tttaagacca agactaccag cgagaataaa
     3901 ggagaattgt tgattttcat aacgccacga gttgtgaaac aggaagtaat ttagaaggag
     3961 aggtaaaatt cgaaaaacat atttaccaaa agacgtaaat gaatcgatat caggtcatca
     4021 taataagtaa tgcgttccta ttgaatgtgg aatagccttt aaacgctgct tggtgtctgg
     4081 agtttacgtt gtaaaattta ataaaaaaaa gaggtctttg caatgagaat aaccggcaag
     4141 ctttttgttg tcttaatcca gctgcttttg gtatcttctt taatttctgt ccacggctgt
     4201 ggtaaggagc taaaaggcca caccagtgga ggatgtccgg atagtacggc gccagacggg
     4261 gccacgataa tagctccgtc gagtctcact ggtcctttta tcaacagttc gacctgttat
     4321 cctactttag gctttacggt gaaagattca gggggtaacc ctatgaacaa cgtctgtgtc
     4381 gaaatattca gtgacgcaac aattgcgctt cattctggcg ctcctgattg cagcaacgta
     4441 gctgccaacc ctcaatcgtc aatggtagca aggacggaca acagtggaaa cgtgattgtt
     4501 gaactgttga ctggaccgac cccaaccgga gggactcatt ttgtgcaggt tacttccggt
     4561 gcattaacgg ccatagcaac cactgcagcg gcacaataga agattagaaa taagtttagg
     4621 catatcgcct tcaatacatt tcaggtcctg ttctgccggc tcatccgaag gtttttctgc
     4681 cgcctttttg cgactctctt ctcttccgct tctttttcag agaggatttc tcataaaagg
     4741 gccggttttt cagctcgcgg aacagcccgt catgttgcat ttaacgcttt cgcaggctaa
     4801 gggcttttcc aatattttta tcatcaactc ttaagcacct tgtttttctc cgcggaacgc
     4861 tcctccctcc gtctcttggc aaataaacgc agcggcgtgc ccctgaatcc gaatgcgtcc
     4921 cgaagaccgt tctcgatata gcgaagatag gagaagtgca caccctcggg gtagttgacg
     4981 aaggcgatga aggtgggcgg tttgaccgcc acttgggtaa gataataata ttttacccgt
     5041 ttaccgcggt agagaggagg ctcgtgggcc gcggtcagct tttcgaaaac gcgattcagc
     5101 tccgccgtag gcaccctttt ctccgcttct ttgatgactg cgtctatttc atcaaagacc
     5161 ttggttatgc gctggcgcgt cacggcggag atagtaagca ccggcgcata gtgggcgaat
     5221 ttgaggcgct gctgtatgtc cgctataaac tttttgtaag ccagctcttt gtctgggacc
     5281 agatcccatt tgttcaggac gaggatgaga cccttccccg cttcattcac gaggcccgcg
     5341 atccgctcgt cctgttcggt tactccttca gcagcgtcga taagcaccag tgccacatct
     5401 gcgcgagaca ggcccttcat ggcccgcatg acactgtatt tttcaacccc ctggccgatt
     5461 cttccgcggc tccggatgcc ggctgtatcg acgaggacgt attttcgccc atagtatcgg
     5521 tattcgctgt ccaccgcgtc cctggtcgta ccggacgtgg gactgacgat catgcggtcc
     5581 tcgccgatga gcgaattcac aaaggacgat tttcccacat tcggacgtcc caggatggca
     5641 acgcggggaa tactgacctg ctcctcatcc tccctgccgg cgggcgtcgc aggaagcatg
     5701 cgccagagtt cgtccatgag gtcggaaaag ccgacgccgt ggagggctga aagggggaaa
     5761 aaggcgtcaa tgccgaggcg gtagaagtcg ggaatgagct cttcgtgccg cgccccgtcg
     5821 accttgttta cagcgtagat cacgggtttt cccgaagccc ggagccggtg cgacacctcg
     5881 atgtcggaag ggagcagacc ctgcctgccg tccatgagga agataatgat gtccgcttcc
     5941 tccacggcga gcgtcgtctg ctcgcgcatc ttcagataca tggggtcctc ggtctccggt
     6001 tcgaagcctc cggtgtccac gagcaggaaa cgtttcccct cccattcggc ctcggcgtag
     6061 ttgcgatcgc gggtgacccc cggcatatct tcgacgatag cgaggtttct gccgaggatc
     6121 cggttaaaga gcgtggattt gccgacattc ggacgaccca ctattgcgat gattggtttc
     6181 tgcataagat ttccgatttc tgacgcgcat cttttccgtc atccccaatg cctctatcgg
     6241 caatccaggc gtcgtccccg cgaatgcggg tattaaaaaa caaagacacc cccgctcgcg
     6301 ctgccgcaca gcctgcattc ccgctaagca ctcataaacg ctgtcgcatg ctggattccg
     6361 gctcgaacca taccgggatg acggacgaga tgatcgttgt tctcttatag cattattacg
     6421 cacgtcaatt caagaaaaaa ccttgcgcat gccggttgtt tgtgttattt tttctacgct
     6481 acgattcttg cgtccaggag gccttcatgc tgagatgcgt tgtgctggcg gcgtgcgtgt
     6541 tcttcattgc ggcgacatcc gcccatgctg aattttaccg atgggtggat aaggatggaa
     6601 aagaattttt tacaaacgag cttgagaagg ttccacagga ataccgcgaa agcgtacaaa
     6661 cagttaaacc ggatgaatcc cgggtgagcg tcggggacag gccgtctgct gtggtcaagc
     6721 ccgggggcct gtccggggaa cacaaggaca agaacggcag gggagaagag tattggcgca
     6781 aaaaagcagc caatctgagg ctcaaactca gggaccagca ggatgaatac aatttcattc
     6841 tcaagaaact ggagaaccag gaccagaatc cgaatcagct cgccgggaaa aagagcagat
     6901 ccgtgtcgag ccttgaaaaa aagaagttga agcttgaaaa agagatagca cagaccagac
     6961 gaaagctcga agtagacctt ccggaggagg cgcgcagagc agatgcctat cccggatgga
     7021 taagagaata aaatcggttc ggagttcaaa gaatcagata ttagcgtgcc gggaggagtc
     7081 gtttggacga acgttacgat tttaaaaaag tcgaagaacg ctggcagcgt tcctgggaag
     7141 aacacaggac gttcaaagtg atcgaggacg cttcgaagcc gaaatactat tgcctcgaga
     7201 tgtttcccta tccgtccgga aggatccaca tgggccatgt gcggaactac gccatcggcg
     7261 atgtagtctc gcgctacaaa cggatgcggg gattcaacgt cctgcacccg atgggatggg
     7321 actctttcgg gctgccggcg gagaacgcag cgatcaaaca caacatccct ccctcgaagt
     7381 ggaccctcga aaacatcgcc ttcatgcggg acaaacagct caaacggctc gggctgtcat
     7441 acgactggga ccgtgaagta acgacctgcc tggaggagta ctaccgttgg aaccagtggt
     7501 tcttcatcag gatgttcgag cgcggtcttg cctataagaa actctcctac gtgaactggt
     7561 gtccgtcgtg cagcacggtg ctcgcaaacg aacaggtgga gaacggccag tgctggcgct
     7621 gcggctcgct ggtgacgcac aaggaactcg accagtggtt ttttaagata accgattacg
     7681 cggaggagtt gcttgcgcac tgcgaccggt tgacgggctg gcctgaacgg gtgcttacga
     7741 tgcagcggaa ctggatcggc aagtccgtag gcgtcgaggt ggacttcgcg atcgagggga
     7801 caggcgagaa actcacgata ttcacgacga ggcaggacac cctcttcggc gtgacgttca
     7861 tgagcattgc acccgaacat cccatgctcg aatctctgat caagggcagg agtcgggccg
     7921 atgacgtccg agccttcgtg cagcgcgtgc tgcgcgagga caaggccgtg cgcaccgacg
     7981 agaaacagga gaaggaaggc atattcaccg gagcttacgc aatcaacccg atgaacggca
     8041 gcaaagtgcc ggtctgggtt ggcaactttg tgcttatgga atacggcacg ggcgcgatca
     8101 tgtccgttcc cgcccacgac cagcgcgatt tcgagtttgc gaagaaatac ggtttgccgg
     8161 tacaggtggt catccagagc cccggcaagg atcttatggc cgataccatg aagcaggcct
     8221 atgtcgagga cggcatcatg gcgaactccg gcgtcttcga cgggcttacg aataccgccg
     8281 cgaaggagaa gatcgcggat tatatcgagc agtccgggct cggcaaacgc accgtaaact
     8341 ggcggctccg cgactggggc atttcacggc agcgctactg gggaacgccg atcccgattg
     8401 tctattgcga tgcgtgcggg acggtgcccg tgcccgataa ggatcttccg gtccggctgc
     8461 cggtagacgt cgagttgacg ggcaaggggc gctctcccct ggccgaatcc aagtcgttcc
     8521 gcgagacggt ctgccccaag tgcggaggga aggcccgtcg cgagacggac accatggaca
     8581 ccttcgtgga ttcgtcgtgg tattttctcc gctactgctc tccccgggag aataaagccc
     8641 ccttcgacaa aaaggccgcg ggctactgga tgagtgtgga ccagtatatc ggcggaatcg
     8701 aacatgcggt gcttcatctc ctgtatgcgc ggttttttac gaaagtggtt cgcgacctgg
     8761 ggctgtttga cggagatgag cctttcaaga acttgctcac ccagggcatg gtgatcaagg
     8821 acggcgccaa gatgagcaaa tccaagggga acgtggtgga ccccgaccat atccttgcga
     8881 aatacggagc ggacactgcg cggctttttt cgctgttcgc cgccccgccg gaacgcgacc
     8941 tcgactggag cgaccagggc gtgga
//
LOCUS       DASSLD010000181         4410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_40672,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000181 DASSLD010000000
VERSION     DASSLD010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40672"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VFK23_03365"
     CDS             <1..450
                     /locus_tag="VFK23_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HET7318156.1"
                     /translation="VYPSTALYDGRGAPLPWLQQMPDPMSTVVWDSWVEINPRTADEL
                     GIKFGDLVEVTSPQGALRVPAVIYPGIRPDMAAMPLGQGHKEMGRYAKGRGVNPLRLL
                     AMIDDKGKPTQPAWNATRVRIKRISEKGDLVVYGNPQGSFRSELIEI"
     gene            600..1319
                     /locus_tag="VFK23_03370"
     CDS             600..1319
                     /locus_tag="VFK23_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7318157.1"
                     /translation="MEKLTSPYKWGMVVDLDRCTGCGACVVACQAENNVAICGKKEIS
                     EGRDMHWLRIERFWVGEYPDVRVRFLPVLCQHCHDAPCEPVCPVYASYHNPDGLNGQV
                     YNRCVGTRYCGNNCPYAVRVFNFYDHEHSWPHPLNYQLSPDITVRSRGVMEKCTFCIQ
                     RIRRAKEESRAEGRLIRDGEVQPACAQTCPSEAIVFGNVADPGSRVSRLSRSPRSFRL
                     LEHLGTHPSVTYLKGGGREHV"
     gene            1312..2691
                     /gene="nrfD"
                     /locus_tag="VFK23_03375"
     CDS             1312..2691
                     /gene="nrfD"
                     /locus_tag="VFK23_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET7318158.1"
                     /translation="MSEMPDHELSAKSAKQVNDELLSSMRPPTFRWYLLIGGLSAVVA
                     WGTLAFAYQVMTGMSVTGKNRPVMWALYITGFVFWVGMSHSGTMVSAILRLSQANWRR
                     PILRAAEAMTVFTISIAALFPFIHLGRNWVVHYFLPYPNQRGLWPNFRSALLWDITAI
                     ATYIIGSSLFLYLGMIPDLAIVRDRTPNGWRKRMYSVLAMGWRGTAREWIVYQKASTL
                     MAALIIPVAVSVHSIVAWDFAVTVVPGWHSTIFPPYFVIGAITSGVAAVITLMIIIRR
                     VFHLEEYLTPVHFDNMGKLLLVICLLWSYAYFVDVNITWYAHEPIEWETFSFMAGKYT
                     PLLLLMLFGNSILPITVLCFKKLRRNVKVMLFVTLLINVCMFIERFLIIVPSLSHKNM
                     PFVWHTYSPSLVESSVNMAALAGFVLLYALFTKFFPIVAVTDVRELGVRHTDVEVGRT
                     KVHSITGEE"
     gene            2741..3250
                     /locus_tag="VFK23_03380"
     CDS             2741..3250
                     /locus_tag="VFK23_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HET7318159.1"
                     /translation="METLGTFKDVESAIRAVDSLIKAGFTEETITSLTSVPYPNGVIV
                     KSNQRTWFRWLSAAGGVIGAAAGFALAAGTAYLYPVQTGDKPIIAYFPTGIITYEITM
                     LFAILGAIGGMFLEMKLPPWREKVYDPAIGEGAIGISVSVDTGDRSTRAETIMKEAGA
                     LRTAVGGNP"
     gene            3247..3702
                     /locus_tag="VFK23_03385"
     CDS             3247..3702
                     /locus_tag="VFK23_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7318160.1"
                     /translation="MKRRSLLLIMASAPLLMAAIWMDDQQSYKPYKAPVLAPPAAAVP
                     VSGKEIVSPESELRNPVAATEASLSQGKILFEVNCAMCHGLTSTERGSVGKKLVPPPP
                     GLDHDMVKGLSDSTIFKAITFGFGRMPAFQDKLMPTERWNLVNFLRTRQ"
BASE COUNT          913 a         1278 c         1262 g          957 t
ORIGIN      
        1 gtctatccct ccaccgcttt gtacgacgga cgcggagctc cgcttccctg gctgcaacaa
       61 atgcccgatc cgatgtccac cgtggtctgg gacagctggg tagaaatcaa ccccaggacc
      121 gccgatgagt tgggaatcaa attcggcgac ctggtcgagg tgacatcacc ccagggcgcg
      181 ctgcgcgtgc cggcagtgat ttatcccggc atcaggcccg atatggcggc gatgcctttg
      241 gggcagggcc acaaagaaat gggacggtat gcgaagggaa ggggagtgaa tccgctccgg
      301 ctcctggcga tgatcgacga caagggaaag cccacgcagc cggcctggaa cgccacacgg
      361 gtgcgaataa aacggatttc ggagaagggg gaccttgtcg tgtacggaaa tccgcagggg
      421 agcttcagga gcgagcttat cgagatctag ggattgatga ttgttgattg tcgattgttg
      481 attgaaacga aatgatgcac ctgcgctctt caatcgcaaa tcgccagtcg ccaattatgc
      541 cgtttctcaa agagatatca ttctgccgca tcaaggttgt tagaagtcag gagtaagtta
      601 tggaaaagtt gacgagcccg tacaaatggg gaatggtcgt ggacctcgac cgctgcaccg
      661 ggtgcggggc ctgtgtggtc gcctgccagg cggagaacaa cgtggccatc tgcggaaaga
      721 aggaaatctc cgaaggccgt gacatgcact ggctcaggat cgagcggttc tgggtggggg
      781 agtacccaga cgtgcgcgtg cgcttcctgc cggtactctg ccagcactgc cacgacgcgc
      841 cctgcgagcc ggtatgcccc gtctacgcca gctatcacaa tcccgacgga ctgaacggcc
      901 aggtctacaa ccgctgcgtc ggcacccggt actgcggcaa taactgccct tatgcggtca
      961 gggtcttcaa cttctacgac cacgagcatt cctggccgca cccgctcaac tatcagctat
     1021 cgcccgatat tacggtccgc tcgcgcgggg tcatggagaa gtgcaccttc tgtatccagc
     1081 gtatccggcg ggcaaaggaa gagtccaggg ccgaaggtcg cttgatccgt gacggcgagg
     1141 tgcagccggc ttgcgcccag acctgcccgt ccgaagcgat cgtcttcgga aacgtggccg
     1201 acccggggag ccgagtatcg cgactttccc gcagtccgcg cagcttccgg cttctggagc
     1261 atctcggcac tcatccgtcg gtaacgtatc tcaagggagg ggggcgcgag catgtctgaa
     1321 atgcccgacc atgaactttc ggccaaaagc gcgaaacaag taaacgacga actgctctcc
     1381 tccatgcggc ctccgacctt tcgctggtac ctgctcatcg gcggcctgag cgccgtggtg
     1441 gcctggggca cactcgcctt tgcctatcag gtgatgaccg gcatgagcgt gaccggcaag
     1501 aaccgtccgg tcatgtgggc gctctatatc acgggcttcg tgttctgggt cggcatgtcc
     1561 cactccggca ccatggtctc ggcgatcctg cgtctttccc aggccaactg gcgcaggccc
     1621 atcctgcgcg cagccgaggc aatgaccgtg ttcaccatca gcatcgcggc cctgttcccg
     1681 ttcattcacc tgggcaggaa ctgggtcgtg cactattttc ttccctatcc caaccagcga
     1741 ggcctgtggc cgaatttccg ctcggccctg ctctgggaca taacggcgat cgcgacctac
     1801 atcatcggca gcagtctctt cctctacctg gggatgatcc ccgatctcgc aatcgtgcgc
     1861 gaccgcacgc ccaatggatg gcggaaaaga atgtattccg ttctcgccat ggggtggcgg
     1921 ggaacggcaa gggagtggat cgtctaccag aaggcctcga ctttgatggc ggccctgatc
     1981 atcccggtgg ccgtttcggt gcattcgatc gtggcctggg acttcgcggt taccgtggtc
     2041 cccggctggc acagcaccat ctttcccccc tatttcgtga tcggcgcaat cacgtcaggc
     2101 gtggcggcag tgatcacgct catgatcatt atccgccggg ttttccacct ggaggagtat
     2161 ctcacgccgg tccacttcga caacatgggc aagcttctgc tcgtcatctg cctgctctgg
     2221 tcctacgcct atttcgtcga tgtcaacatc acctggtatg cccatgagcc gatcgagtgg
     2281 gagaccttca gtttcatggc gggcaaatat acaccgctgc tgctcctgat gcttttcggg
     2341 aactcgatat tgccgatcac ggtcctctgc ttcaaaaagc tgcggcgcaa tgtcaaggtc
     2401 atgctcttcg tcacgctcct gatcaacgtc tgcatgttca tcgaacggtt cctgatcatc
     2461 gtgccgtcgc tctcgcataa gaacatgccg tttgtctggc atacgtactc cccctcactg
     2521 gtggagtctt ccgtcaacat ggcggccctg gccggcttcg tgctgctcta cgccctgttt
     2581 acgaagtttt tcccgatcgt ggcggtgacc gatgtgcggg aactcggggt ccggcatacc
     2641 gatgtcgaag tgggccgcac caaggtgcat tcgattacgg gggaggagta gtatcgaatg
     2701 cggaataaaa attgtgaaag gactgaataa ggtgcggcaa atggaaacgc tcggaacatt
     2761 caaggatgtt gagagtgcga tcagggcggt ggacagcctg atcaaggcgg gatttaccga
     2821 ggagacgatc acctcactca cgtccgttcc atatcctaac ggcgtgatcg taaagtccaa
     2881 ccaacgtacc tggttccgct ggctttccgc ggcaggcggc gtcatcggcg ctgcagcggg
     2941 ctttgccctg gctgcgggaa ccgcttatct ttatccggtt cagaccgggg acaagccgat
     3001 catcgcgtat ttccccacgg gcatcatcac gtacgagatc accatgctct tcgccatact
     3061 gggagctatc gggggcatgt tcctcgaaat gaagcttccc ccctggaggg aaaaagttta
     3121 cgatccggca atcggagaag gcgcaatcgg tatcagcgtc tccgttgata caggggaccg
     3181 gagcacgagg gccgagacga tcatgaaaga agccggggca ttacggacgg cagtgggagg
     3241 gaacccatga aacgacgctc gctcctgctc atcatggctt cggctccact gctcatggcg
     3301 gcgatctgga tggacgacca gcagtcctac aagccgtata aagcaccggt cctggccccg
     3361 ccggctgcgg ccgttccggt ttcaggcaag gaaatcgttt cgccggagtc cgagctcagg
     3421 aatcccgtgg ccgcgaccga ggcatcgctg tcgcagggga agatactttt tgaggttaat
     3481 tgcgccatgt gccacggact tacatcgacg gaacgaggtt ccgtcggcaa gaaactcgtt
     3541 ccgccgcctc ccggtctcga ccacgacatg gtcaaaggcc tcagcgattc caccattttc
     3601 aaggcaatta ccttcggttt cggccgcatg cccgccttcc aggacaaact catgcccact
     3661 gaacgttgga atctcgtaaa tttcctccga actaggcaat agctgcccat ccatacatac
     3721 ccggtctttt cttctggcac tgccacatcc tctcgcatgg ggataacgaa atgataaggc
     3781 cctactgcgt cggaggcgga ttcaatccgt agcacgcatg acagcgtgtt gtaattagaa
     3841 ggacgatcaa acagggaggg ggccgcaagg ctcccttttt gcctaatcta tataatatat
     3901 tacatattgc gaattgaccg gcctattcag attgacctac ccatagtgaa ctgataatga
     3961 atttttttgt ctgcaacttg tgcaagtggc tgtttttaaa gcgcatcgac ggagatcgcc
     4021 tctggcacgc cctttgcttt tgttctaaat gggttatcgc gccaccgagc gtccgcccca
     4081 tttttacggc aaccgtccct cccgttacgg ccgccaacct ggtcgtgacg gtgaatttta
     4141 attcggagca gactaattga tataatgagt attgaatctt ccgtgtcccg tccgcgacgg
     4201 gccaggaggc cgcgggaaaa aagagcccca tcttgcgtaa gcgcggcttg aaagagcggg
     4261 ggatcgccac atgatgcgaa aaaagtatct tcacacttac tggctgccgg ccgccgttgt
     4321 gctgttcgga ctgctgggtg ctgtcctgtt cacctggaca aaccatatca atgaaaagca
     4381 gcgggtcaac tatttgctgc tcgatgcgat
//
LOCUS       DASSLD010000182         3161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_114585,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000182 DASSLD010000000
VERSION     DASSLD010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114585"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VFK23_03390"
     CDS             <1..726
                     /locus_tag="VFK23_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HET7318161.1"
                     /translation="HFYMGLCYKMALDFDKAASMFRAVLDMNRDFVSEADAEWAVIQK
                     IQRAAPGTEIGKKIALIDKIDRADVAALFDQEMNLEKLFTKRGTKTYDTGFKAPTESS
                     TRMETETTTKMDPATDIADHWLKPSIDTVLKLQIRGLEAGPNHKFEPDKLITKGEFAL
                     MLEDILVKVTGDEKLATKFLGATSPFPDVRNDHYAFNAIMIVTSRGFLEADKATGEFK
                     AGEPVSGADALLAIREFKNQLKF"
     gene            786..2165
                     /locus_tag="VFK23_03395"
     CDS             786..2165
                     /locus_tag="VFK23_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HET7318162.1"
                     /translation="MRTFRHAIILILLLSCSRGAEASLKILEGEHTVVYDIVNPRGVA
                     FIPDYSDESFEQKVISQDEFSKRVRVTSRMAPMKTRVPFPVPPRMLSASVSRYLSPER
                     DRQSDHPSIVRLAGDITRGSRYAHEAANAVLSWVADNLTFDASVTVPADALSALRYKR
                     AYCVGYSNLTVALLRAAGIPARVAHGYLPPGYEWGFSKEYWGVKVNDGGFHAYLEIYY
                     PDTGWVFSDAEHAHNFVDPFHIILRLDGMDMPGAYQGGYLEVDKATFYTIFQEEDKTV
                     MVDELPTPREKRLGRRLDNLQHAALVSGRVTDRAGQPVPKGSVVLWKEGRGTAAPFSD
                     GRFAASVTKDGRYRVEVKGPGFAKSSQEQQVEQGKVYTMNFTLSPGGVIKGKVLNAAG
                     KPLAEGDVFYKDGGTSYGVSVDRDGAYKIEGLAPGSYKVSAVIGEREVSRMVQVEAGR
                     ETIADFLIK"
     gene            2193..>3161
                     /locus_tag="VFK23_03400"
     CDS             2193..>3161
                     /locus_tag="VFK23_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318163.1"
                     /translation="MNKRIITALFIALLTSSGSTWAQNGHMGDAVEQTSSGLINWTSG
                     EVYATGIGAPPAKATNAAQARAMAERAAFVVALRNLLETVKGVRVDSETVIENFMVTS
                     DVIRTRVDGIVKGARSVKTSYMSDGSVEIQVVMPLKGALMDALVPENFGRPAGMPAPQ
                     KPAASLQQPSKPAIPAPAPAKPAPPSKPEPKKPSNAAPLPAVPEKKPEQPKSSVPPLA
                     AVPEKKSEPQKQEEPQKQPEPSKQATPSEGEQTAQFKGGVATGLVIDGRGLGLRPALL
                     PKILDSQGREIYVGQVATRTNAVEQGVAGYAKDVKAAENNFRVTDNP"
BASE COUNT          690 a          937 c          974 g          560 t
ORIGIN      
        1 catttttata tggggctgtg ctacaagatg gcgctcgatt tcgacaaggc ggcgtcgatg
       61 ttccgcgcgg tcctcgacat gaatcgtgat tttgtcagtg aggcggacgc cgaatgggct
      121 gtgatccaga agatccagcg cgcggcgccc gggaccgaga tcggcaagaa gatagccttg
      181 atcgacaaga tagaccgggc agacgtcgcc gccctcttcg accaggagat gaacctcgaa
      241 aaactcttca ccaagcgggg cacgaagacc tacgacacgg gtttcaaggc cccgaccgag
      301 tcgagcacca ggatggagac ggaaacgacg accaagatgg accccgcgac cgatatcgcc
      361 gaccactggc tcaaacccag catagatacg gtgctcaagc tccagatacg ggggctcgag
      421 gcgggcccga accacaaatt cgaaccggac aagctcatca caaaaggtga atttgcgctc
      481 atgctcgaag acatcctcgt caaggtgacg ggcgatgaga agctggccac gaaattcctc
      541 ggggccacct cgcccttccc cgatgtgcgc aacgaccatt acgcgttcaa tgcgatcatg
      601 atcgtgacgt cgaggggttt cctggaagcg gacaaggcga cgggggaatt caaggccggc
      661 gaacccgtgt ccggggccga tgcgctcctc gcgatccgcg agttcaagaa ccagttgaaa
      721 ttctgatcga cgcgagtcga cgcccgctcc tgctcctgcg gggcgggcgt tttttttgat
      781 ttatcatgcg aacgttccgg catgcaatca tactgatcct gctcctgtcc tgttcccgag
      841 gcgccgaggc aagcctcaag atactcgaag gcgagcacac ggtcgtttac gacatcgtga
      901 acccgcgcgg cgtggccttt atccccgatt attccgacga atcattcgaa cagaaggtga
      961 tcagccagga tgagttcagc aaacgcgtgc gggtgacgtc ccggatggcg ccgatgaaga
     1021 cgcgcgtgcc ttttccggtc cctccccgga tgctctccgc ttcggtgtcg cgatacctct
     1081 cgccggaacg ggaccggcag tccgaccatc cgtcgatcgt ccgcctggca ggcgacatca
     1141 ccaggggcag ccgctacgcc catgaagcgg cgaatgccgt tctttcctgg gtggcggaca
     1201 acctgacctt cgacgccagc gtcacggtcc ccgcggatgc cctttccgcc ctcaggtata
     1261 aaagagcgta ttgcgtcggg tattcgaacc tcacggtggc gctgctccgc gctgcgggca
     1321 tcccggcccg cgtcgcccac ggctacctgc ctccgggata cgagtggggc ttttcaaagg
     1381 aatactgggg cgtgaaggtc aacgacggcg gtttccatgc ttatctcgag atctattatc
     1441 cggacacggg atgggtcttt tccgacgcgg agcatgccca taactttgtc gatccctttc
     1501 acatcatcct tcgcctcgac ggaatggaca tgccgggcgc gtaccagggc ggatacctgg
     1561 aagtggacaa ggccaccttt tataccatct ttcaggaaga ggataagacg gtcatggtcg
     1621 atgaactgcc gacgccgaga gagaagcggc tcggccggcg gctcgataac ctgcagcatg
     1681 cggcgctcgt gagcggccgc gtgacggacc gcgccgggca gccggtcccc aaaggttcgg
     1741 tcgtactctg gaaagagggg agagggacgg cagccccctt ctcggacggc aggtttgcag
     1801 cctccgttac gaaggacggc aggtaccggg tggaagtcaa aggacccggt tttgcaaagt
     1861 cctcgcaaga gcagcaggtg gagcagggaa aggtctatac tatgaacttt acgctgtctc
     1921 cgggcggcgt gataaaaggg aaggtcctca acgcagccgg caaacctctt gcggagggtg
     1981 acgtctttta taaggacggc ggtaccagct acggcgtctc ggttgacagg gacggcgcct
     2041 ataaaataga aggcctggct cccggatcgt acaaagtttc cgccgtgatc ggcgagcggg
     2101 aggtttcccg catggtgcag gtcgaggccg gcagggaaac catcgcggat tttctcatca
     2161 aataattttt ccggcgcatg gggtaaagga tcatgaacaa acgtattata accgcactgt
     2221 tcatcgctct gctaacgtcc tcaggttcga cgtgggccca gaacggccat atgggagatg
     2281 ccgttgaaca aacaagttcg ggcctgatca actggacttc gggagaggta tatgcaaccg
     2341 gcatcggcgc tcctccggca aaggcgacca acgccgcgca ggcccgcgcc atggccgagc
     2401 gagccgcttt tgtcgtcgcc ctcaggaacc tcctggaaac ggtaaagggc gtccgcgtcg
     2461 attccgagac ggtgatcgaa aacttcatgg tcacgagcga cgtgatcagg acgcgggtcg
     2521 acgggatcgt caaaggcgcc aggtccgtca agacgtcgta tatgtcggac gggtctgttg
     2581 aaatccaggt cgtcatgccg ctcaaagggg ccttgatgga tgccctggtg ccggaaaact
     2641 ttggaaggcc cgccggtatg cccgcgccgc agaaaccggc tgcctccctg caacagcctt
     2701 caaaaccggc aatccctgcc ccggcgcctg ccaagccggc ccctccgtcg aaaccggagc
     2761 ctaaaaaacc ttcaaacgct gcacctctcc ctgcggtgcc ggagaaaaaa ccggagcagc
     2821 cgaagtcgtc tgttccgcct ctcgcagcgg tgccggagaa aaaatcagag ccacagaaac
     2881 aggaggaacc gcagaagcag ccggagccgt cgaaacaggc aacgccgtct gaaggagaac
     2941 agacggccca gtttaaggga ggcgtggcga ccggcctcgt gatcgacggc agggggctgg
     3001 gactgcggcc ggcgctcctt ccgaagatcc tggattccca gggccgggaa atctacgtcg
     3061 gccaggtggc cacccggacg aacgccgtcg agcagggggt tgcgggatat gccaaggacg
     3121 tcaaagcggc cgagaacaac ttccgggtca ccgacaaccc g
//
LOCUS       DASSLD010000183         3238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1120325, whole genome shotgun sequence.
ACCESSION   DASSLD010000183 DASSLD010000000
VERSION     DASSLD010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120325"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1344
                     /locus_tag="VFK23_03405"
     CDS             130..1344
                     /locus_tag="VFK23_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HET7318164.1"
                     /translation="MKWRKQKEKIFLMACLTTVLTFGYQVGHAASTLAISPAGDGVFV
                     IQGAGIENAGAMDFTVLYDTSSFANPRVVQGALISGALMAVNDRTPGVVRLAIIRTTP
                     IQGSGVIATLTFDRRGQSAGKNPSLVVNKLTNVEGNPISAGATNDTSGTSQKQETGGE
                     PSVPGVSAAPGAPSAPVQPGVILLPGGGVAPEGKKDQTAGPQPETPAEDATASAKEPS
                     AVPQGITASLEPQKKKVQQFMSVLERFRKNDKERTAKAFLSFFDQVEANGFRQEPSVA
                     LSDGKATVKIYFVVPSSGGAAPDFALSGARLVSIKKDADRSNTWMIEALPEKGGYEAS
                     LTVSQGDVMLDYPLVLAPKIDLKEGSKPVTDEDFGRYLSGKRKDLNGDGKRDFLDDYI
                     FTANFIAARQKK"
     gene            1543..2145
                     /locus_tag="VFK23_03410"
     CDS             1543..2145
                     /locus_tag="VFK23_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7318165.1"
                     /translation="MDVKPITVLLVEDNDDDVVLIRKALAGAMKVPYVLKQVDGLSRG
                     MERLAGGDIDVVLLDLGLPDGRGMSTFSVLHKQSPDVPVIVLTGHDDEELAIEAVQKG
                     AQDYLVKGKVDGGLLGRSIRYAIERQKLSTQLKQSMKEINTLRGFLPICANCKKIRDD
                     RGYWTQIETYISKHSEAEFSHGLCPECMVRLYGRELGGKT"
     gene            2364..>3238
                     /locus_tag="VFK23_03415"
     CDS             2364..>3238
                     /locus_tag="VFK23_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7318166.1"
                     /translation="MVYLERFTPPFDRLEPWLMPWKTVIPPEANLDRRLSLMQDQFEA
                     SSRVAYYSLVVMIVSFAALSMYFSFVYQQKQRRARENMLLVLKNQEIARRNEFIRYIS
                     ATIGHEFKNNLGRIKRRIDLLPGLPAEALERIDDNLDKLFADIDIFRKISDKREAGLV
                     EFKSVDIEAMLGDLALHYNDFADIAVAGATAVPMIYAAPPLLKTVFENLIDNSIKYKK
                     TDQPRARVRVACEIDRDGTRRYVTISFRDEGIGMDEQQADRCFYKGRTTGEGWGQGLY
                     YVKYVIGLHAGKIRIG"
BASE COUNT          711 a          925 c          969 g          633 t
ORIGIN      
        1 ggagtcattc cggccttttt tattggattc ctcatgcatg ggcgtccatc tcgtgttatg
       61 atttgatttc cgttttgctt ttcgttccgg tataatgtta gcagcatttg ttcgggaggg
      121 ttcctaatca tgaaatggcg caagcaaaaa gaaaaaattt tccttatggc ctgcctgacg
      181 accgttctga cgttcggata ccaggtgggt catgccgctt ccacgttggc aatctcgcct
      241 gccggcgacg gcgtttttgt aatacaggga gcgggaattg aaaacgcggg ggccatggac
      301 ttcaccgttc tgtacgacac ctcgtcgttt gccaacccgc gggtcgtcca gggtgcgctc
      361 atctccggcg cgctgatggc cgtgaacgac aggacgcccg gcgtggtgcg gctcgcgatc
      421 atccgcacaa cgcccatcca gggaagcgga gtcatcgcga ctctgacgtt cgaccggaga
      481 ggacaatcgg ccgggaaaaa tccgtccctc gtggtgaata aattgaccaa cgtcgagggg
      541 aatcccattt ccgcgggcgc cacgaacgac acatcgggca cttcacagaa acaagagaca
      601 ggcggagagc cgtccgtccc cggggtatcg gcagcaccag gtgcgccgtc cgcaccggta
      661 cagcccggcg tcattctcct gccgggcggc ggtgtcgcgc cggaaggaaa gaaagaccag
      721 accgcggggc cgcagccgga gacgcccgcg gaagatgcca cggcctctgc aaaggagccg
      781 tccgcggtac ctcagggcat aacggcttca ctggaaccac agaagaagaa agtccagcag
      841 ttcatgagcg tgctggagcg tttccggaag aacgacaagg aacggacagc caaggccttc
      901 ctctcctttt tcgaccaggt tgaagcaaac ggcttcaggc aggagccatc agtcgcactg
      961 tccgacggca aggcgacggt gaaaatctac ttcgtcgtac cgtcgtccgg cggcgccgcg
     1021 ccggatttcg cgctcagcgg cgccaggctc gtttcgatca agaaggacgc cgaccgctcc
     1081 aatacctgga tgatcgaggc gctgccggag aagggcgggt atgaggcgag cctcaccgtg
     1141 tcccagggcg acgtcatgct ggattacccc cttgtcctgg cgccgaagat cgacctgaag
     1201 gaaggatcaa aaccggtgac ggatgaggac ttcggccgat acctgagcgg gaagagaaag
     1261 gacctgaacg gtgacggcaa gcgtgatttt ctcgacgatt acatctttac ggcgaacttc
     1321 atagctgcaa ggcagaagaa ataaaatcgt cgtagctgcc tggtatctct aagttcgtcg
     1381 tccccgaatg cttctatcgg gaacccagcg ccttccggaa tctctggatt cccgcaagac
     1441 aaacgcatcc ccacccctac ccctcccctt caaggggagg gaaaaaggga ggggatgggt
     1501 ctacgttcgg gaatgacgac tttttgcact gagagtgttc tcatggacgt caaacccatc
     1561 accgttctgc tcgtagaaga caatgacgac gacgtcgttc tcattcggaa ggccctcgcc
     1621 ggcgccatga aggtccccta cgtgttgaag caggtcgacg ggctgagccg gggcatggag
     1681 cgtctggccg gcggtgacat cgatgtcgtg cttctggacc tggggcttcc ggacggccgc
     1741 ggcatgagca ccttttcagt gctgcacaaa cagtcgcccg atgtcccggt gatcgtgctg
     1801 accggccatg acgatgagga gcttgccatc gaggccgttc aaaaaggcgc acaggactat
     1861 ctcgtgaagg gcaaggtcga cggcgggctc ctggggcggt ccatacgata cgcgatcgaa
     1921 cggcagaaac tgagcaccca gttgaagcag tcgatgaagg agatcaatac gctgcggggg
     1981 tttctgccca tctgcgccaa ctgcaagaag atccgcgacg atcggggcta ttggacccag
     2041 atcgagacct atatcagcaa gcattcggag gcggaattca gccacggcct ctgccccgaa
     2101 tgcatggtaa ggctctacgg acgggaatta gggggaaaaa catgagacag tcgagacccc
     2161 acccgtcgat ccccgccgtc aacgccgttt gaaagaatcg cggaacccac tgagccggca
     2221 agcagccggc tcattatttt tcctgttgta ttagcgggcc gtttggaata aattattcag
     2281 gctatgtctg aacgcgacat tcccgataag atccggggcc tggtgctctc ggccctgttg
     2341 atagtgctct ggcttatcgt ggcatggtct acctggagag attcactccc cccttcgacc
     2401 ggctcgagcc gtggctcatg ccctggaaga cggtgatccc tcctgaggcc aatctcgacc
     2461 ggcgcctttc cctcatgcag gaccagttcg aggcgtccag cagggtcgcc tattactccc
     2521 ttgtcgtcat gatcgtgtcc ttcgccgctc tgagcatgta cttttccttc gtctaccagc
     2581 agaagcagcg cagggcgcgg gagaacatgc tgctggtgct caagaatcag gagatagccc
     2641 ggcggaacga attcatccgg tacatctctg cgacgatcgg gcatgagttc aagaacaacc
     2701 tggggaggat aaaacgcagg atcgatctgc tccccgggct gcccgcggag gcgctggaac
     2761 gcattgacga taatctggac aagctttttg ccgacatcga tatcttcaga aaaatctccg
     2821 ataaacggga agcgggcctc gtggagttca agagtgtcga catcgaggcg atgctcggcg
     2881 atcttgcgct tcactacaac gacttcgccg atatcgccgt ggcgggggcg acggccgtac
     2941 ccatgatcta cgccgctccc cctctcttga agacggtctt tgaaaatctg atcgacaatt
     3001 ccatcaagta taaaaaaact gaccagcccc gcgcgcgcgt gcgcgtagcg tgcgagatcg
     3061 accgggacgg caccaggcgc tacgtgacga tctcgttccg ggacgaaggg atcggcatgg
     3121 acgaacagca ggcggaccgg tgtttttaca aggggaggac caccggcgag ggatggggcc
     3181 aggggctcta ctacgtcaag tacgtcatcg gcctccacgc cggaaagatc aggatcgg
//
LOCUS       DASSLD010000184         2770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_939479,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000184 DASSLD010000000
VERSION     DASSLD010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939479"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFK23_03420"
     CDS             complement(<1..609)
                     /locus_tag="VFK23_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET7318167.1"
                     /translation="MSGMETYLAGSPLVAVGAAFASGILAGLSPCVYPMIPIVTGYVG
                     GRAVKERAFCEHCGPVTNIEAIVAGPGGRTRARYFFLSLGYVFGMAAVYSLLGMIAAL
                     TGDFFGRISTSPWALLFVANIFILFALNLLDVVPFPAWFQGRSMKPAMGGVIGAFLIG
                     AVSGLVASPCTSPVLFALLTFVATSRSVVYGGVLLFAFSMGMG"
     gene            complement(702..1931)
                     /locus_tag="VFK23_03425"
     CDS             complement(702..1931)
                     /locus_tag="VFK23_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318168.1"
                     /translation="MSVLIKTCTRAAGATFRHLPAVTLIILFIFEGAFIARASEKEQA
                     APGELITEPSPTPPPAQPETEQIAPGEPITAPSPTPPPAQPETEQIAPENLLTAPSFT
                     APPAKPEEVTKPKLPAAKELKYVDMLHSEIVRRLLASAVWLDSFFADTEYLKEVNYSY
                     VRFRYEVFKEERSSVTFRPAVDFRLALPALEKKTNLVFSADPAEPAPGAAGAPVRTAA
                     ERFGTSAQSSVTAALQYIFKQDAQTSLLVRTGFQFVKLSPVILLAPRYRVLMPLDTWA
                     MRFTQEVIWKSRGSWQSDTRFDFERPLPYELFFRTSIDGLWAAQKNGYAYSLAFSLHQ
                     PLDPTHALDYEWINSYNTYPRGELTEVALRVRYRHSFLRDWLFFELAPQVRFPRDANF
                     DSTAGILFKFEAFFGKI"
     gene            2148..>2770
                     /locus_tag="VFK23_03430"
     CDS             2148..>2770
                     /locus_tag="VFK23_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7318169.1"
                     /translation="MFVVELVMQGVRGIRDLSRVRFQSGFNLVVAGNESGKTTAVDTL
                     QRLLFPNDGVGAMEHLVSKHTPDSSRGALVLCADDGAYYRVIQDFHRRAVNLSWYDAS
                     SKEFNLRYKDWNESAQFMAGLTAGVAEDDFARVFIFRREDAAGRTAPPLQAAVAAPID
                     RPKRGAAGGDAAGKAKIAELRETLRKAEEAADAEYRYQSAKLTLDEIG"
BASE COUNT          603 a          805 c          856 g          506 t
ORIGIN      
        1 acccatcccc atggaaaaag cgaacagcag gacgcctccg tacacgacgc tccggctcgt
       61 cgcaacgaac gtaagcaggg cgaagaggac cggtgaggtg cagggcgatg cgaccagccc
      121 cgaaacggcc ccgataagga atgctccgat gaccccgccc atggccggtt tcatcgagcg
      181 accctgaaac caggcgggaa aggggaccac gtccaggaga ttcagggcga acagaatgaa
      241 aatgttggcg acgaaaagca gggcccaggg gctggtgctg atcctcccga aaaaatctcc
      301 ggtcagcgcc gcgatcatgc cgagcaggga atagaccgcg gccatcccaa aaacgtatcc
      361 gagcgaaaga aagaaatatc tcgctctcgt tctccctccg gggccggcaa cgatcgcttc
      421 aatattggtc acgggaccgc agtgttcgca aaatgccctt tccttgacgg ccctgccgcc
      481 gacatatccc gtcacaatgg gaatcatggg atagacgcag ggggaaagtc cggcgagtat
      541 gcccgatgca aaggccgctc cgaccgccac gagcggagat cccgccagat aggtttccat
      601 accgctcata ccgttgttgc tccgtcacgc ccggcattac gcaatctgct cactttaagt
      661 ataactccga tcagtgataa ggccatgagt acttcgattt tttaaatctt cccgaagaat
      721 gcctcgaact tgaacaggat gccggccgtc gaatcgaagt ttgcgtcgcg cgggaacctg
      781 acctgggggg cgagctcgaa gaagagccag tcgcgcagga agctgtggcg ataccggacc
      841 cggagggcga cctccgtaag ctcgcctctg ggataggtgt tatagctgtt gatccactcg
      901 taatcgagag cgtgagtcgg gtccaggggc tggtgcagcg agaacgcaag gctgtaagca
      961 tagccgttct tttgcgcggc ccataagccg tctatgctcg tgcggaaaaa aagctcgtag
     1021 gggagcggcc gctcgaaatc aaaccgggtg tctgactgcc agctcccccg cgacttccaa
     1081 atgacttcct gcgtaaacct catcgcccag gtgtcgagcg gcataagaac ccggtagcgc
     1141 ggggcgagga ggatgaccgg ggacaatttt acgaactgga atcccgtccg tacgagtaag
     1201 ctcgtctgcg cgtcctgctt gaaaatatac tgcagtgccg ccgtaacgga actctgagcc
     1261 gacgttccaa aacgctccgc tgcggttcgg accggcgccc cggcggcacc gggggcgggt
     1321 tcggcgggat ctgccgaaaa gacgagattc gtcttcttct cgagcgccgg aagggcgagc
     1381 cgaaaatcga cggccggtct gaatgtgacg ctggaccgtt cctccttgaa tacctcatag
     1441 cggaaccgca cgtaggaata gttgacttcc ttcaagtatt ccgtatcggc aaaaaaggaa
     1501 tccagccata cggcgctggc aaggagacga cggacgattt cgctgtgtag catatccaca
     1561 tactttagct ccttagcagc cgggagcttg ggctttgtta cctcctccgg cttcgccggc
     1621 ggggccgtga aggagggcgc cgtaagcaga ttctctggtg cgatctgctc cgtttcgggc
     1681 tgtgccggag ggggcgtggg ggaaggcgcc gtgatcggtt ctccgggcgc gatctgctcc
     1741 gtttccggct gcgccggagg gggcgtaggg gagggctccg tgatcagttc gccgggagca
     1801 gcctgctcct tctccgatgc ccgggcgatg aacgcccctt caaagatgaa gaggatgatc
     1861 agcgtgacgg ccggcaggtg ccggaacgtc gccccggctg cccgggtgca tgttttaatc
     1921 aatacggaca aattaaggat tgtgctcctg attccctgat agtccggcgg cgcaaaccgc
     1981 gccgtcggtt catagccgat gcggagaaaa gaccgtcttt aaaaaggata ggcaatagag
     2041 cattgaatgt caagggataa agcacggtaa gcgccttttc cctgttgcaa cttgcggcta
     2101 cttgcgttat agtaatcaac cgaataacgg ggaaaggagt cgtgtccatg ttcgtcgtgg
     2161 agcttgtgat gcagggcgtc cgcggaatcc gggatctgtc acgcgtcaga ttccagagcg
     2221 gattcaacct tgtcgttgca ggcaatgaat cgggaaaaac cacggccgtc gacaccctgc
     2281 agcgtctcct gtttccgaat gacggggtgg gggccatgga acacctcgtg tcgaagcaca
     2341 cacccgactc ctcgcgaggc gcgcttgtgc tctgcgccga cgatggcgcc tactaccgcg
     2401 tgatccagga cttccacagg cgcgccgtga acctctcctg gtatgacgcg tcgtccaagg
     2461 aattcaatct gcggtataag gactggaacg aatcggcaca attcatggcc ggcctcacgg
     2521 ccggggtggc cgaagatgac ttcgcgcggg tattcatatt ccggcgcgag gacgccgccg
     2581 gccggaccgc gccgcccctc caggctgcag tcgccgcgcc catagacagg cccaaacgcg
     2641 gcgctgcggg cggggacgcg gccggcaagg cgaagatcgc cgagctccgt gaaaccctcc
     2701 gcaaggcgga agaggccgcc gatgcggaat accgttacca gtccgccaag ctcactctcg
     2761 acgagatcgg
//
LOCUS       DASSLD010000185         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1513587, whole genome shotgun sequence.
ACCESSION   DASSLD010000185 DASSLD010000000
VERSION     DASSLD010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513587"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VFK23_03435"
     CDS             complement(<1..896)
                     /locus_tag="VFK23_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318170.1"
                     /translation="MKITREAFEEYSLAQSAVPALDMFKPKAIPAVEPAPETEPTAAT
                     REAAGFAEAANEEPKPTEPSSPAVPDFLQEREEPSIPLPNPFEETSPLTEQTPLEEPP
                     ALHSPLPEEMRTEPVKEPEVAIEPAPRSEPVREAEPKQEPAPEPAAESKHETAVEGPD
                     AGLVSVPVPEQAPDVVTLETPAVTNGETGPAVMAKPAATAVSAAARVSVHEKAAPRKE
                     PAHAAVTMTPKPVIGTVASQPVRSGRMVPLLLAAAIILVLAGFGAYLYVRPSPPSVVH
                     EAGPVMVSTEGLQIINPAGSLDA"
     gene            complement(1169..1378)
                     /locus_tag="VFK23_03440"
     CDS             complement(1169..1378)
                     /locus_tag="VFK23_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007420811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HET7318171.1"
                     /translation="MTENEPVKKIRCPICKKEAAWRDNPFRPFCSERCRLIDLGKWAS
                     EDYRIPGEKKEVPEGEDDEKNKGGR"
     gene            complement(1421..>2051)
                     /locus_tag="VFK23_03445"
     CDS             complement(1421..>2051)
                     /locus_tag="VFK23_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318172.1"
                     /translation="VLSLVHFDTPGDVNGQAVLENIWKYLGGKIAVKDAGYTICDEEK
                     LELRAVHRDASRPESIVSEMECMVKAIVELGCRNFLWFWRNSMLLQWRRGVRGLEYCT
                     LFIMMKELAEALRAGAASCTLSDLERIRGLLLPFADKAKRLLLMERYAMQNGHITYEK
                     CDDPVIQSIREELFSRSKSHGGLFKRLLDEIDGSLFRVLTETRTRKGVS"
BASE COUNT          412 a          603 c          536 g          500 t
ORIGIN      
        1 gcatccagag aaccggccgg attgatgatc tgaagcccct cggtcgaaac catgacggga
       61 cccgcttcgt gcactacaga cggcggagaa ggccggacat acagatatgc cccgaacccg
      121 gccagaacca gtatgatggc cgcagccaga agcaggggga ccattctgcc ggaacggacg
      181 ggttgagacg caactgttcc tattacgggt ttcggcgtca tggtgacggc ggcatgggct
      241 ggctcttttc tcggtgccgc cttctcgtga actgagacgc gggcagccgc tgaaaccgct
      301 gttgccgcgg gttttgccat gacagccgga ccggtctccc cgttagtcac ggcgggtgtt
      361 tccagcgtta cgacatccgg cgcctgttcc ggcaccggca cggataccaa tccggcatcc
      421 ggtccctcca ctgccgtttc gtgttttgat tcggctgccg gctcgggcgc gggctcctgt
      481 ttcggttccg cttccctgac cggttcagac cgcggtgcgg gctcgatcgc gacttccggc
      541 tccttcaccg gctccgttct catttcctcc ggcaacggtg agtggagggc cggcggctct
      601 tcgagtggcg tctgttccgt caagggagaa gtttcttcga aaggattagg caggggaatc
      661 gacggctcct cccgttcctg caggaaatcg ggtacggctg gggatgaagg ctcggttggt
      721 ttcggttcct cgtttgcagc ttccgcaaag cccgctgcct cccttgtggc ggcggtcggt
      781 tccgtttcgg gtgcaggctc aaccgcgggt atggccttgg gcttgaacat atcgagcgcg
      841 ggaaccgcag attgagccag ggaatactcc tcaaacgcct ccctggtgat cttcacggat
      901 tcgccacaac gcgtacagga gaacttgacg cccctgccgg ttattttgtc atccgaaaca
      961 cgaaagagct tattgcactt tccacaccgg acttccatcc tgagcggcct cctggagaag
     1021 atagggacac taattcgaat ttctcatttt gcaacaatat cgaactactg caggacgcat
     1081 cccttaaatt ttgaggtttg aatttgtttg ggatttcgtg tctcgaagtt acgatccgac
     1141 ccattgtaat atattttaaa aaaattatct accgccctcc tttgtttttt tcatcatcct
     1201 ccccttccgg aacttccttc ttctccccgg ggatgcggta gtcctccgat gcccattttc
     1261 cgaggtcgat caacctgcat cgctcggagc agaaagggcg gaacggattg tcccgccagg
     1321 cagcctcctt tttacagatc gggcatctga ttttttttac aggctcgttt tccgtcatcg
     1381 gtccgtataa gaaaggtttg acgctttgcc cgggatgcgt tcatgacacg cccttccgcg
     1441 ttctggtttc cgtcagcacc ctgaacagcg acccgtcgat ttcatcaaga agccttttaa
     1501 acaacccgcc gtggctcttt gaccgggaaa aaagctcctc cctgatcgat tgaatcaccg
     1561 ggtcatcgca cttttcatag gtaatgtgtc cgttctgcat ggcatagcgt tccatcagga
     1621 gaagacgctt cgctttatcc gcaaagggca gaaggagccc cctgatgcgc tccagatcgg
     1681 ataaagtgca cgatgccgcc ccggctctca gcgcctccgc cagttccttc atcataatga
     1741 agagcgtaca gtactccagt ccccgcacac cccttctcca ctggaggagc attgaatttc
     1801 tccaaaacca caggaagtta cggcatccga gttcgacgat agctttaacc atgcattcca
     1861 tctcggaaac gattgattcc gggcgtgaag catcacgatg cacagcgcga agctcaagtt
     1921 tttcttcatc acagatagtg tatcctgcgt ccttcacagc aatttttccg ccgagatact
     1981 tccagatatt ttccaatacg gcttgaccgt ttacatcgcc cggcgtatcg aaatgaacca
     2041 gagagaggac c
//
LOCUS       DASSLD010000186         3600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1285795, whole genome shotgun sequence.
ACCESSION   DASSLD010000186 DASSLD010000000
VERSION     DASSLD010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285795"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..298)
                     /locus_tag="VFK23_03450"
     CDS             complement(14..298)
                     /locus_tag="VFK23_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009802569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HET7318173.1"
                     /translation="MTKAELVDKIAEKKPGLTRKQVEVVVNTVLDGIKDALSREDKVE
                     IRGFGSFRVRHRRAKEGRNPKTGETVQVPPKKVPFFKAGKEMREMVDGRI"
     gene            complement(393..1289)
                     /gene="sppA"
                     /locus_tag="VFK23_03455"
     CDS             complement(393..1289)
                     /gene="sppA"
                     /locus_tag="VFK23_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET7318174.1"
                     /translation="MKRNPLLIIIATALALGVSFVAILALVTYLSGGRRTPAPLAVGG
                     GNVALVKIDGLLVNSEDVVEELTDYSEDSSIKAIVLRIDSPGGGVVASQEIFNAVKNA
                     KKEGKKIVASMGSVAASGGYYVAAAADKIVANPGTLTGSIGVKMEFASVEKLLEKIGV
                     KGLVVKAGEYKDIGSPFRDMTDHEKQLLQGVIDDVHSQFIEAVSEGRGLPETDVKAIA
                     DGRIFTGRQALALKLVDRLGDLADSIKLAGTLAGIKGKPRVVEKRKKLTFLDYLKEES
                     AAWIGEVITKGLGGNTLSLQYR"
     gene            complement(1297..2991)
                     /locus_tag="VFK23_03460"
     CDS             complement(1297..2991)
                     /locus_tag="VFK23_03460"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET7318175.1"
                     /translation="MGDTGEHIDHEENSKATGQETEFEQLYNQSVKQFKSGTLVRGRV
                     LQIRSDFVMLDVGYKSDGIIPVEQFTEDEIKTMKPGDELEVILEAAEDSNGNIMLSRD
                     KAKKLQVWDDLNRAYQTGSPVSGKVLAAIKGGLSVDIGGVEAFLPGSQADIKPVQNSA
                     RLVGQTLEMRIIKMNSGRGNIVLSRRAILEQQQNLKKEATLSALAEGQIVSGVIKNIT
                     DYGAFVDLGGIDGLMHITDISWSRLNHPSEVLKVGERVDVVVLKYDREKQKVSLGMKQ
                     KTPDPWLSVAEKYPVGSRVRGKVMSLTDYGAFVELEHGVEGLVHVSEMSWTQKIKHPS
                     KVVSVGDMIEAQVLSVDPAGKRISLGLKQTTPNPWQTIGERHPVGSEIEGKVKTVTDF
                     GAFVGIEEGIDGLIHISDLSWTRHVKHPSEVLKKGQKTKAVVLALDPAKERISLGLKQ
                     LTPDPWDKAIPERYKVGQDEKVKVTKKADFGMFVELEHGIEGLIPASEIPKDSPEIKE
                     GDEVTARVIKVDRGDRKIALSIKAHIKGQDRSSLREFMKQQEKPDTSIGALLKERS"
     gene            complement(3055..>3600)
                     /locus_tag="VFK23_03465"
     CDS             complement(3055..>3600)
                     /locus_tag="VFK23_03465"
                     /inference="COORDINATES: protein motif:HMM:NF014456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET7318176.1"
                     /translation="GKAQRDAEALNKEGYQVVVVGEKKHPEVQSILGYAGDDSVVLET
                     ADDVDGTDLRPRVGVVAQTTQSYSNFSEIVLKLLRLSKELKVFNTICSSTKERQEAAR
                     VLARRVDVMLVVGGRNSANTTRLASLCREEGKPAYHIEVAEEIRPEWLEGISSVGVTA
                     GASTPDWVVDGVLERLKAIKT"
BASE COUNT          613 a         1151 c          960 g          876 t
ORIGIN      
        1 ttgaaaaagt gaattatatc ctgccgtcga ccatttcccg catttccttg cccgccttga
       61 agaagggcac tttttttgga ggcacctgga cggtctcacc ggtcttgggg ttcctgcctt
      121 ccttggcccg acggtggcgg acccggaagc tcccgaaacc ccggatctca accttgtctt
      181 cccgtgacag ggcgtccttg atgccatcga gcaccgtgtt cacgacaacc tccacctgtt
      241 tcctggtcaa tcccggcttc ttctcggcta tcttgtctac cagttccgcc tttgtcatgc
      301 aatgtcctcc tgataataaa gttcagggtt cagagttgga gtgttccact actttgaact
      361 ttgaactatg aactttgaac tgcttatgag tcctatcgat actggagcga cagcgtgttc
      421 ccgccgagcc ctttcgtgat gacctctccg atccaggccg cggactcttc cttcaggtaa
      481 tcgaggaacg taagcttttt ccgtttctcg acgaccctcg gcttcccctt gatccctgcg
      541 agcgtccccg cgagctttat actgtcggca aggtctccca gccggtcgac gagtttgagc
      601 gcgagcgcct ggcgcccggt gaagatgcgg ccgtcggcga tggccttgac gtccgtttcc
      661 ggcaggcccc tcccctcgga tacggcttcg atgaactggc tgtgaacgtc gtcgatgacg
      721 ccctggagca attgtttttc gtggtcggtc atgtcccgga acggcgaccc gatgtccttg
      781 tattcccccg cctttaccac cagccctttg acgccgatct tctccaggag cttctcgacg
      841 ctggcgaact ccatcttgac gccgatgctg ccggtgagcg tccccgggtt ggccacgatt
      901 ttgtccgcgg cggcggccac gtagtaccct cccgaagccg cgacgctgcc catcgatgcg
      961 acgatctttt tcccttcctt ttttgcgttc ttgacggcgt taaagatctc ctgagacgca
     1021 acgacgcccc ctccggggct gtcgatccgg agcacgatgg ctttgatgga cgaatcttcg
     1081 gaataatccg tcagttcctc caccacgtct tcggagttca cgaggaggcc gtcgatcttc
     1141 acgagcgcca cgttgccccc tccgaccgcg agcggagccg gtgtccgcct gcccccggag
     1201 agataggtga ccagcgccag aatggcgacg aacgacactc ccagcgccag ggcggtcgcg
     1261 atgatgatca gcaggggatt gcgtttcacg cgcggatcag ctccgttctt tcagcagcgc
     1321 cccgatgctc gtatcgggct tttcctgctg cttcatgaat tcccgcaggc tcgaccggtc
     1381 ctggcccttg atatgcgcct tgatggaaag cgcgatcttg cggtcgcccc ggtccacctt
     1441 gatgacgcgg gcagtcacct cgtcgccttc cttgatttcc ggtgagtcct tcgggatctc
     1501 cgacgccggt atcaacccct cgatgccgtg ctccagttcg acaaacatgc cgaaatcggc
     1561 ttttttcgtc accttgacct tttcgtcctg gccgaccttg taccgctccg gaattgcctt
     1621 gtcccagggg tccggcgtca gctgcttgag ccccagcgat atgcgttctt tcgcagggtc
     1681 aagggcgagg accacagcct tggtcttctg gcctttcttg agcacctcgg agggatgctt
     1741 gacatgcctg gtccacgaca ggtcggagat atggataagg ccgtcgatgc cctcctcgat
     1801 gccgacgaac gcgccgaaat ccgtgacggt cttcaccttg ccctcgatct ccgaacccac
     1861 gggatgccgt tcgccgatgg tctgccaggg attgggcgtg gtctgcttca agccgaggga
     1921 aatccgcttg cccgcggggt cgacggaaag aacctgcgct tcgatcatgt ccccgacgct
     1981 cacgaccttg gacggatgct tgatcttctg ggtccaggac atctccgata catggaccaa
     2041 cccttcaacg ccgtgttcca gctccacgaa cgcgccgtag tcggtcaggc tcatcacctt
     2101 gcccctcacc ctgctgccga cggggtactt ttccgcaacc gagagccagg ggtcaggggt
     2161 cttctgtttc attcccaaag acaccttctg cttctccctg tcgtacttga gcacgacaac
     2221 gtcgacccgc tcgccgacct tgagcacctc ggaaggatga ttgagtctgc tccaggatat
     2281 gtcggtaatg tgcatgagcc cgtcgatgcc gccaaggtcg acaaacgcgc cgtagtcggt
     2341 gatgttcttg atcaccccgc tcacgatctg gccttcggcg agagcggaga gggtcgcttc
     2401 ctttttcagg ttttgctgct gttccagaat ggccctgcgg gagagtacga tattgccgcg
     2461 gccggagttc atcttgatga tcctcatttc cagcgtctgg ccgaccagac gggccgaatt
     2521 ctggacgggc ttgatgtccg cctgggaccc cgggagaaac gcctcaacac cgccgatatc
     2581 gacggacaat ccgcccttga tggcggcaag cacttttccc gacaccggtg atccggtctg
     2641 gtaagcgcgg ttcaggtcgt cccagacctg aagtttcttg gccttgtcgc gtgagagcat
     2701 gatgttccca ttcgagtctt cggccgcctc gagtatcact tccagttcgt cgccgggctt
     2761 catcgtcttt atctcgtctt ccgtgaactg ttccacgggt atgatgccgt ctgatttgta
     2821 tcctacgtcc agcatgacaa agtcggaccg gatctgcagc acccgtccgc ggaccagggt
     2881 gccggacttg aactgcttga cggactggtt gtagagttgt tcgaactccg tttcctgtcc
     2941 ggttgccttg ctattctctt cgtgatcgat gtgctctcct gtgtcgccca tgaaacttcc
     3001 ctacctcccg aaagtaaatt ccctgttgct gctgtctgtc atttaaaaat gcgattaagt
     3061 cttaatggcc ttcaatcttt ccaaaacacc gtccacgacc cagtcgggcg tggaggcccc
     3121 ggccgtcacc ccgaccgagc tgatgccttc cagccactcc ggccggattt cctcggccac
     3181 ctcgatatga taggctggct ttccttcctc ccgacacaaa gacgcaagcc tcgtcgtatt
     3241 cgcgctgttg cggcccccca cgaccagcat gacatcgacc cgcctggcga gaacgcgggc
     3301 cgcttcctgc cgctccttgg ttgagctgca aatggtgttg aagaccttta attccttgga
     3361 aagacgcaat aactttagca caatttccga aaaattgcta tatgattgcg tggtttgcgc
     3421 cacgacaccg acccggggcc ggaggtccgt gccgtctaca tcgtcggccg tttccaggac
     3481 gacggagtca tcaccggcat agccgaggat gctctgcacc tcggggtgct ttttctcgcc
     3541 cactacgacg acctgatatc cttccttgtt gagcgcttcc gcgtcgcgct gcgcctttcc
//
LOCUS       DASSLD010000187         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_627483,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000187 DASSLD010000000
VERSION     DASSLD010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627483"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VFK23_03470"
     CDS             complement(<1..858)
                     /locus_tag="VFK23_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318177.1"
                     /translation="MKSNSKRERPLTSITGGCPALFFLVSAIFLSVMALPFPSLAGDS
                     LAEMARIEASDIVTIDGTRSLFKGYMSLGLLPEAASLLERRVRLGVFPAEAAAPLFDE
                     VVSAQGHFDEPERLVAVCETAIRSGIRTPLILYTYGTGLRGVRGRLGDASATLAQIET
                     EDVYRLLALYSLGQIAAGRGEAGTALDLFRRVEEGAGGVERGGFLAVRAARSRAELLL
                     SSGKPDDAAPVFEALLRKEGNPLDRIGLAAAGDNSVHVLEHLPAEMIAGRPLEDRVRF
                     LLLLGGVARK"
     gene            complement(836..1150)
                     /locus_tag="VFK23_03475"
     CDS             complement(836..1150)
                     /locus_tag="VFK23_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HET7318178.1"
                     /translation="MVGTGIRVADTFVSRLRGLLFTKSLGPGEGLWIRPCNSIHTFGM
                     RFEIDALFLGPDGKVVGRYERFRRNRISRVYWNARGVLELPAGTIERTGTEVGDEIQF
                     ET"
     gene            complement(1354..2310)
                     /locus_tag="VFK23_03480"
     CDS             complement(1354..2310)
                     /locus_tag="VFK23_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7318179.1"
                     /translation="MIYLVSFSIFLATILVACALYWWLETRSESLTQRLQEEVGKESD
                     SRTEKWLDAIRNRFGALKGLFSVKPAQETELLERLSSGKELTGARLLLNQAGYRGTGA
                     YHTYLVVRSALPAALVVLAILYAKGIGLTNQSTFLLLLVFGIVGFLLPDFVLRRKIHK
                     RQEEIQDALPDGLDLLVVCVEAGLGLNAAFIKITEEFKLSSPALSEEFDIVNREMVAG
                     KPRQEALRALSERTGVEDVKSLVAMLIQTEKLGTSLSQSLRVHSDSLRTRRRQRAEEA
                     AAKTTIKLVFPLVFLMFPALFIVILGPGAITIIKAFSPMVGK"
     gene            complement(2324..>2801)
                     /locus_tag="VFK23_03485"
     CDS             complement(2324..>2801)
                     /locus_tag="VFK23_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7318180.1"
                     /translation="GHSFTGAIQLVAQGMPVPIGPEFSKVFDEQNLGIPLRQALIGMT
                     DRVDILDVKFFVTAILIQRETGGNLAEIIDKIAYVIRERFRIHGQLKIFTAQARMTGY
                     ILSLLPIGVAVLIGMMNPDYMKPLWFEKIGRIMIAIAVILQILGMLAIRKIIRIKI"
BASE COUNT          592 a          882 c          789 g          538 t
ORIGIN      
        1 ttttcttgca acccccccta gaaggagcag gaaccggacc cgatcctcaa ggggacgccc
       61 ggcgatcatc tcggccggca gatgctcgag aacatgcacg gaattgtctc cggcggcggc
      121 cagaccgatc cggtccaacg ggtttccttc cttgcgtagc aaggcctcga acaccggcgc
      181 cgcatcgtct ggcttcccgg acgaaaggag caattccgcc cgcgatctcg ccgccctcac
      241 cgcgaggaaa ccgccccgtt ccacgccccc tgcaccctcc tccacgcgcc ggaagaggtc
      301 aagggccgtc cctgcttccc ctctccccgc tgcgatctgg ccgagagagt acagggcgag
      361 aagccggtac acgtcctccg tttcgatctg ggccagtgtc gcggaggcgt ccccgagccg
      421 tccccggacc cctcgcaacc ccgtgccgta ggtatacaga atcaggggtg tccgaatgcc
      481 gctgcgaatg gcggtctcgc agactgcgac gagacgctcc ggttcatcga aatgcccctg
      541 tgcgctgacg acttcatcga agagcggcgc cgccgcctcc gcggggaaca cgcccaggcg
      601 gaccctccgt tcgaggagcg atgccgcctc ggggagaagc cccagagaca tgtatccctt
      661 gaaaagagag cgtgtgccgt cgatggtcac aatatcggac gcctcgatgc gcgccatttc
      721 cgcaagggag tccccggcaa gcgacgggaa gggcaaggcc atgaccgaaa ggaagatcgc
      781 ggagacgaga aagaaaaggg cgggacaccc gccagttatg gaggtcagtg gcctttcacg
      841 tttcgaattg gatttcatcc ccgacctcgg tccccgttct ctcgatggtg cccgccggca
      901 gctcgaggac cccccgggcg ttccagtaga ccctggaaat gcggttcctg cggaaccgtt
      961 cgtaccgtcc caccaccttc ccgtccggac cgagaaagag cgcgtcgatt tcgaaacgca
     1021 tcccgaacgt gtggatgctg ttgcacggcc ggatccagag cccctccccg ggtccaaggc
     1081 ttttcgtgaa cagcagtccc ctcaagcgtg agacaaacgt gtcggcaacc cggattcccg
     1141 tccctaccat ggaactccgg gtcttgttcc aagctgccgg catccggctc taccggggga
     1201 aaacgctact tcttcttgct ggtccttcgg gatctcacgt aaatatagat tccgaggacg
     1261 accacaaaaa gcaccatcca tggcagggct gcattcagat tcatcgatta cctcccctga
     1321 ttaatgcgta tgtgcgttgt ttttcccccg cgttcatttg ccgaccatgg gagaaaaggc
     1381 cttgattatt gtgatggcgc ccggcccgag tatgacgatg aacaacgccg ggaacatgag
     1441 gaataccaac gggaatacga gcttgatggt cgtcttcgcc gcggcctctt ccgccctttg
     1501 ccggcgtcgc gtacgcaggg aatccgaatg tacccgcaat gactgtgaga ggctggtccc
     1561 gagcttctcc gtctggatca gcatggcgac gagagatttc acatcttcga caccggtccg
     1621 ttcggaaagt gcccggagcg cttcctgccg gggcttaccg gcgaccatct cccggttgac
     1681 gatgtcgaac tcttcgctca acgcaggact ggaaagcttg aattcctccg taatcttgat
     1741 aaaagcggcg ttcagtccca acccggcctc gacacagacg accaggaggt cgagcccgtc
     1801 gggcagtgca tcctggatct cttcctgacg tttgtgaatt ttccgccgga gaacgaaatc
     1861 cggcaacaga aacccgacga tcccgaacac gagaagcagc aggaaggtcg actgattcgt
     1921 taaaccgatt cctttggcgt agagaatcgc caggaccacc aaagcggccg gcaatgccga
     1981 gcgaaccaca agataggtgt ggtacgctcc ggtcccacgg tatcccgcct ggttcagcag
     2041 aagacgcgcg cctgttagct cctttccgga ggaaagcctt tccagaagtt cggtctcctg
     2101 ggccggcttg acggaaaaaa gtcccttaag tgcgccgaac cggttccgaa tcgcgtccag
     2161 ccacttttca gtgcgcgaat ccgactcctt gcccacctct tcctgcaacc gttgggtaag
     2221 cgattcactt cgagtttcga gccaccagta cagcgcgcac gctacgagga tcgtcgccag
     2281 aaagatggaa aacgagacaa ggtatatcat gctctccccc aactcagatc ttgatccgga
     2341 tgatcttgcg gatggccagc atcccgagga tctgcaagat caccgcgatc gcgatcatga
     2401 tccttccgat cttctcgaac caaagcggtt tcatgtaatc cgggttcatc atcccgatga
     2461 ggacggcgac cccgatcggt agaagcgaga ggatgtatcc ggtcatacgc gcctgggcag
     2521 tgaagatctt gagttggccg tgaatgcgaa atcgttcacg gatcacatag gcgatcttgt
     2581 cgatgatctc cgcgaggttt cctcccgttt ccctctggat caggatcgcc gtgacaaaga
     2641 acttgacgtc gaggatatcg acccggtccg tcatcccgat gagagcctgc cgaagaggaa
     2701 tccccagatt ctgttcatcg aacaccttcg agaactcggg gccgatcgga acaggcatcc
     2761 cctgggcgac gagctggatg gcgcccgtga aagagtggcc g
//
LOCUS       DASSLD010000188         3325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1408633, whole genome shotgun sequence.
ACCESSION   DASSLD010000188 DASSLD010000000
VERSION     DASSLD010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408633"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..910
                     /locus_tag="VFK23_03490"
     CDS             110..910
                     /locus_tag="VFK23_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7318181.1"
                     /translation="MPDNSPLTPKDIILSGKRVRVFTSGTGPALLLLHSAWGDAEMSW
                     SPVWSGLSESFTVIAPDLPGFGASESLDMPTLAANAHVMKELLDALKINAAIVAGNSF
                     GAAVAIEFASSFPQHTLCVVLVNGGYLPLIPGFLRSLISLPIAEKRFRAFMRNFNYSD
                     KAFAKAFPNPDKLPPLFFDRIRRNEEKQSRIVFDTFMRQTQSQMPPPVPTAMIWGTGD
                     RLVTMRQAGIIRKWLGNPDFVPIDGAGHMPQVERPSEFVEAMKKIGQM"
     gene            1280..1957
                     /locus_tag="VFK23_03495"
     CDS             1280..1957
                     /locus_tag="VFK23_03495"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7318182.1"
                     /translation="MLIFGAFLIAGPFTVIHLNISEPQALLWDGFLSALFFLQHSGMI
                     RSSFRTWLSSIIPAHYYSATYAIASGTVLTAVVLLWQPSHTTLLEVQGLLRMLFRAAF
                     LLAIAGFVWGVRALGEFDPFGRLSILSHLNDRQQASHLVVRGPYLWVRHPLYFFTLVL
                     IWATPSLSLDRLLFNVLWTFWIVLGSCLEEKDLVAEFGESYRHYQDTVPMLIPWRCPV
                     GRISKTP"
     gene            complement(2194..>3325)
                     /gene="hemG"
                     /locus_tag="VFK23_03500"
     CDS             complement(2194..>3325)
                     /gene="hemG"
                     /locus_tag="VFK23_03500"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HET7318183.1"
                     /translation="NRLHSLPEGVMLMVPTSFWPMAKSHLISWPGKIRMGMEVFVPKH
                     RYPDDESLASFVTRRLGRECLEKIAEPLVAGIHTSNPDNMSVLATFPRFVEMERKSGS
                     LILAMLAAMKSRPHATLSGPPPKPGAPKMTYFMSFKNGMQTLSQACVDYIGKDSISTA
                     APVTAIEPKGKGYAVVLESGETIAADHVIIASAAYDAAGMVKGFDTTLADELNRLEWS
                     SSSTVSIAFRREDVKVPLKGFGFIVPRVEGRRVNAATYSSIKWSYRGPDDHVLIRVFV
                     GGGHHEELVHELDDAGMVATVLEELDVILGLKADPTFSRVYRWYKGMPKYTVGHLDRI
                     AHLDRTLSAHPGLHLIGCSYKGIGIGDCIHEAQIAAEKILKC"
BASE COUNT          646 a          938 c          983 g          758 t
ORIGIN      
        1 gatagcttac tccaagtttt atctcgaact tcttgaccaa aaaactgatc ttgtgctact
       61 gtattctgaa gaactgatat atgtttgctc tattcaggag aacaaaatta tgcctgacaa
      121 ttctcctcta acgccgaaag atatcatact ctcgggaaaa cgggtccgcg tctttaccag
      181 tggaacaggg ccggcgctct tgctgctcca ctccgcctgg ggagacgcgg agatgagttg
      241 gtcgcccgtg tggagcggac tcagcgagtc cttcaccgtg atcgcgcccg acctgcctgg
      301 tttcggagca tcggaatcgc tcgatatgcc gacgctcgcg gcaaacgccc atgtcatgaa
      361 ggaactgctt gacgccctga agatcaacgc agccatcgtg gccggcaact ccttcggcgc
      421 tgccgtagcc atcgagttcg cctcgtcctt tccccaacat actctctgcg tcgtgctggt
      481 gaacggcggg tacctgcctc tgattccggg tttcctgaga tcgctgatct cccttccgat
      541 cgcggaaaag cgtttccgcg cgttcatgcg gaacttcaat tactcggaca aggcgttcgc
      601 aaaggccttc ccgaaccccg acaagctgcc ccccctgttt ttcgaccgga tacgccggaa
      661 cgaagagaag caatcacgga tcgtcttcga tacgttcatg cgacagacac aatcgcagat
      721 gccgccgccg gtccccaccg cgatgatctg gggtacggga gacaggctcg tcaccatgag
      781 gcaggcgggg atcatccgca aatggctcgg caatcccgac ttcgtgccta ttgacggtgc
      841 gggccatatg cctcaggtgg aacggccttc ggagttcgtg gaggcaatga agaagatagg
      901 acagatgtga aaaaacttcc agattattcg tgaagagtgc aagaagggtc aagtaagcgt
      961 cgaggaacta agacaaggga accgtcggtg tcgggtatgt gaaacccggc tggcaattgc
     1021 acgtcgaagc aggaaagagc tggggctaat tgggaactga gatcgcaagg cgggtaggag
     1081 tgaacacgtc aagcgtcaac cgtgtgcttg cggggctcga taaatcagca taaggatgca
     1141 taagcacctt gagaaccagc atcccaatgt ttccttcagc cgggccgttg ggccacggaa
     1201 ggacagactg atgcagtcag cgaatcgatt tgcagcttat ttgctaatcg tcttagccac
     1261 tctttctggc ggaggctcat tgctgatctt cggtgctttc ctgatcgccg ggcctttcac
     1321 cgttatccat ttgaacatct cggaaccgca ggcgttgcta tgggacgggt tcctgtccgc
     1381 actgttcttc cttcaacata gcgggatgat acgatcgtca tttcgtactt ggctttcgtc
     1441 cattatcccc gcccactact actccgcaac gtatgcaatc gcatccggca cagttctcac
     1501 cgccgttgtg cttctctggc agccatcgca cacgaccctt cttgaggtcc aggggctttt
     1561 gcggatgctg tttcgcgccg ccttcctcct tgccatcgcc ggttttgtgt ggggcgttcg
     1621 ggcgcttgga gaattcgacc cctttggccg gctctccatt ttatcgcacc tgaacgacag
     1681 gcagcaggca tcgcacttgg ttgtccgggg cccctatctc tgggtccgtc acccactgta
     1741 tttcttcacg ttggttctca tctgggcaac tccctccttg agtttagacc gtttactctt
     1801 caatgtgctc tggacattct ggatcgttct cggttcttgc cttgaggaga aagacctcgt
     1861 tgctgaattc ggcgagagct atcgtcacta tcaagacacg gttccgatgc tcattccgtg
     1921 gaggtgccct gttgggagga tttccaagac gccatgaaat aatgcattcc tggcctggaa
     1981 tggttgtgcc atgctgcctt tccctgcggt cagttcaccc aaccgctgca gccggctgct
     2041 tcgggcggcg gatcatcggg gagttggtga cggcacggga agtgcgggga tgttccggtg
     2101 agatgagtcg gacaagcagg agaataacaa cggcggggtc aaatggcccc gccgtctgca
     2161 ttttgtttca atggtcagtt tgtatcccgc ttctcagcac ttcaggattt tttccgcagc
     2221 gatctgcgcc tcgtggatgc agtcccctat gccgatgccc ttgtaggaac agccgatgag
     2281 gtgcagcccc gggtgggctg aaagggtgcg gtcgagatgg gcgatgcggt ccagatggcc
     2341 gaccgtgtac ttcggcatgc ccttgtacca gcggtacacc cgggagaacg ttgggtccgc
     2401 cttgagcccc agaataacgt cgagctcttc caataccgtg gcgaccatgc cggcatcgtc
     2461 gagttcatgc accagttcct cgtggtggcc gccgcccacg aagacgcgga tgaggacatg
     2521 atcgtcgggg ccgcggtagg accacttgat ggagctgtag gtcgcagcgt ttactctccg
     2581 tccctcgacc cgggggacga tgaagccgaa tcccttcaag gggaccttga cgtcctctct
     2641 gcggaaggcg atcgagacag tggaagagga ggaccattcg agccgattca actcgtccgc
     2701 gagcgtcgtg tcgaacccct tgaccatccc ggcggcatcg taggccgcgg acgcgatgat
     2761 gacatgatcg gcggctatgg tctcgccgct ttcgaggacc acggcatatc ctttgccctt
     2821 gggttcaatc gccgtgaccg gggcggcagt gctgatagag tccttgccga tgtagtcgac
     2881 gcaggcctgg gacagggtct gcatgccgtt cttgaagctc atgaagtagg tcatcttcgg
     2941 cgcgcccggt ttgggcgggg ggccggagag cgtagcgtgc ggccggctct tcatcgcggc
     3001 aagcatggcg aggatgaggc tgccgctctt gcgctccatt tccacgaagc gcggaaaggt
     3061 cgccaggacg ctcatgttgt ccgggttcga ggtgtggatg cccgccacga gcggttcggc
     3121 gatcttttcg aggcattccc ggcccaacct gcgcgtgaca aaggaggcaa gactttcgtc
     3181 gtcagggtac ctgtgcttcg gcacgaagac ttccatgccc atgcggattt tgccgggcca
     3241 ggagatgagg tgcgacttgg ccatgggcca gaacgacgtg gggaccatga gcatgacgcc
     3301 ttcgggcagg gagtggagcc tgttc
//
LOCUS       DASSLD010000189         2732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_669489,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000189 DASSLD010000000
VERSION     DASSLD010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669489"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1855
                     /gene="nadB"
                     /locus_tag="VFK23_03505"
     CDS             281..1855
                     /gene="nadB"
                     /locus_tag="VFK23_03505"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HET7318184.1"
                     /translation="MDYTEEHIQTDFLIIGGGVAGLRAAVELARSGEVLVVTKDAPSE
                     SSSEYAQGGVAVALSDEDEISIHFEDTIRAGDGLCLRKAVGTLVEEGPTRIRELIMWG
                     AEFDREGTKLAFTQEAAHSKRRILHAQGDSTGHEIMRVLIDKVRSSKNAAKIDFAFTR
                     DLIVTDGRCTGAVVCQQAHRKMLVISARAVLLATGGAGQLYERTTNPAVATGDGIAMA
                     YRAGATLMDMEFVQFHPTALYRAGAPQFLLSEAMRGEGGMLRNVSGERFMDRYHPARE
                     LAPRDVVTRAIHAEMAATGSSHVYLDMTHLNADYIIRRFPRIYRTCRNLGIDIAREFI
                     PVSPAAHYLMGGVRTDLWGATTVPGLFAAGETACTGVHGANRLASNSLLEGLVFGERA
                     GLAAARYAQKRIRRAAPPSVSAGRRKTEDRVAEQARAAEIRKALKKIMWEKVGIVRNR
                     KGLAAAQRQLREWERAMKGCGPDRNVFELRNMITAAALITRSALLREGSVGAHYRNDF
                     PAKGRNWKKRTVITKK"
     gene            complement(1911..2480)
                     /locus_tag="VFK23_03510"
     CDS             complement(1911..2480)
                     /locus_tag="VFK23_03510"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HET7318185.1"
                     /translation="MKRFMILVCVALLLTACYPVLNRELMREGSRDVSFDLLRDSPDA
                     YAGRLFILGGIIVNTKFMETGSQIEAMFVPVDSSGYLKERTHAQGRFLALYPRSRGLL
                     DPLVYKRGREITLAGSFLEIRKGKIDEMEYAYPVFEIRQIYLWEEGRYGPYSPYYYPY
                     YYPYYYNSPFLYDPWGRPYPNPFWPMPPW"
     gene            2601..>2732
                     /locus_tag="VFK23_03515"
     CDS             2601..>2732
                     /locus_tag="VFK23_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HET7318186.1"
                     /translation="MTSSKPKIKCFTLSTCSHCHRTKRFFRERGIEIEFTDVDLLTGE
                     "
BASE COUNT          601 a          807 c          836 g          488 t
ORIGIN      
        1 cggccgtcaa ggacggcggc agggtgaccc tggtcttcga accggacagc tttaaacttg
       61 cgtcgatcga cgggaagaac cccttccttg cccagccggg agagacgccg gggagtaaat
      121 aaacatttcg gaatgcggaa tggggaatga aagacgaaga ttcttagagt caactcattc
      181 cggactccct aaaccgcaat cgacagtagt tgcggatttc tgatgcaagg aacgcttgca
      241 cctttttctc attccgcatt ccgaattcca catttttaat atggactaca ccgaagagca
      301 catacagacc gattttctga tcatcggcgg gggcgtggcg gggctccgcg cggcagtgga
      361 actcgccaga agcggcgagg tcctcgtggt gaccaaggac gcgccttcgg agagcagcag
      421 cgagtacgcc cagggcggcg tggccgtggc gctttcggac gaggacgaaa tttcgatcca
      481 tttcgaagac acaatccgcg cgggcgacgg actctgcctc cggaaggccg tcggcacgct
      541 cgtcgaggag gggcccacgc gcatccgcga gctcatcatg tggggggccg agttcgaccg
      601 ggagggcaca aagctcgcct ttactcagga ggcggcccat tcgaagagac ggatcctcca
      661 cgcccagggg gactcgacgg ggcatgagat catgcgggtc ctgatcgaca aggtacgctc
      721 ctcaaaaaat gcggcaaaga tagacttcgc cttcacgcga gacctgatcg tgaccgacgg
      781 ccggtgcacc ggcgcggttg tctgtcagca ggcgcacaga aaaatgctcg tcatctcggc
      841 tcgcgccgtg cttctggcca cgggcggagc gggacagctc tacgagcgga cgacgaaccc
      901 cgccgtcgcg acgggagacg gcatcgccat ggcgtaccgg gccggcgcca cgctcatgga
      961 catggagttc gtacagttcc atcccactgc actctaccgc gcgggcgcgc cgcagttcct
     1021 gctctccgag gccatgcgcg gcgagggcgg catgcttcgg aacgtgagcg gcgagcgttt
     1081 catggaccgc taccacccgg ccagggagct cgcgccgcgc gacgtggtta cccgcgcgat
     1141 ccacgccgaa atggccgcaa ccgggtcgag ccatgtctat ctcgatatga cgcacctgaa
     1201 cgccgattac atcatccggc ggttcccccg catctaccgc acctgcagga acctcggcat
     1261 cgacattgcc cgcgagttca tcccggtctc tccggccgcg cactacctca tgggcggcgt
     1321 caggaccgac ctttggggcg ccaccaccgt ccccggcctt ttcgcggcgg gtgagacggc
     1381 ctgcaccggg gtccacggcg cgaaccgcct ggcgagcaac tcgcttctgg aaggactggt
     1441 gttcggcgaa cgcgccgggc tggcggcggc acgttatgcc cagaagcgga tcagacgcgc
     1501 agcgcccccg tccgtgtcgg ccggacggcg caaaaccgag gaccgcgttg ccgaacaggc
     1561 ccgggcggcg gaaatcagga aggcgctcaa aaaaatcatg tgggaaaaag tcgggattgt
     1621 gcgaaacagg aaaggtctgg ctgcggcaca aaggcagttg cgcgaatggg aacgggcgat
     1681 gaagggctgc ggtccggaca gaaatgtctt cgaactgaga aatatgatca ccgccgccgc
     1741 gttgatcacc cgctcggccc tgttacgcga aggcagcgtc ggcgctcact accggaacga
     1801 cttcccggcc aagggaagga attggaagaa acgaacggtg atcacgaaga agtaacgggt
     1861 tgaaaagacg acggataccc cttccgttcc cggtgaaaag cggaaccctc ctaccatggc
     1921 ggcatgggcc agaacgggtt cggatagggc cgcccccacg ggtcgtagag aaacggagaa
     1981 ttgtagtagt agggatagta gtaaggatag tagtacggtg aatacggtcc gtaccgcccc
     2041 tcctcccaga gatagatctg cctgatctca aaaaccggat aggcgtactc catttcgtct
     2101 atcttgccct tccgtatttc gaggaaggat cccgcaagcg ttatttccct ccctctctta
     2161 tacacgagag gatcgagcag gccccgggac cggggataga gcgcaaggaa ccttccctgc
     2221 gcatgcgtcc tttccttcaa gtatccggag gagtccacgg gcacgaacat cgcctcgatc
     2281 tgggatccgg tctccataaa cttggtgttc acgatgatgc cgccgagtat gaagagcctc
     2341 ccggcatagg cgtccgggga gtcccggagc agatcgaacg aaacgtcccg gcttccctcc
     2401 cgcatcagtt cgcggttcag taccggataa catgccgtca aaagcagcgc cacgcacacg
     2461 agtatcatga atcttttcat gtgattatta tcccatattc ggcgagaggg tgtcaaggcg
     2521 ggcctcgagc gccaaaatcg attgcatgaa aaggcttcgc catgttatac tgtccccgtc
     2581 gtgaccggga gaataattat atgacctcat ccaaaccaaa gataaagtgc tttaccctga
     2641 gtacgtgctc ccattgccat cggacgaagc ggtttttccg ggaaagaggg atcgagatag
     2701 agttcacgga cgtcgatttg ttaacgggtg ag
//
LOCUS       DASSLD010000190         2805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_627268,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000190 DASSLD010000000
VERSION     DASSLD010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627268"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VFK23_03520"
     CDS             <1..632
                     /locus_tag="VFK23_03520"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7318187.1"
                     /translation="STGMVGQLGLLIKNTLLAILNLFIMLFVIFFFFRDGKAYARELT
                     DILPLPRNHKKSIMEKLGNTFSAVLNGLFLVALLQGFMTGVGFFLFGVPYGIFWGFLA
                     MIASLLPFGGTALVWIPGALYLFLKGSTLKAVLLTVWGVLLVSTPDNFLKPILIGRKA
                     KLPTFFLFLGILGGIHVYGILGILFGPMIVTLLTAFVQIYREEYGEQQG"
     gene            797..1480
                     /locus_tag="VFK23_03525"
     CDS             797..1480
                     /locus_tag="VFK23_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET7318188.1"
                     /translation="MEDLAKSDTWRVFRIMAELVEGFEALNGVGPAVTIFGSARLPTG
                     SPYYNKCLKVAERLAQDGFAVISGGGPGIMEAANKGAQNAQGVSVGLNIDLPLEQTPN
                     QFQDIRVEFRYFFVRKLMFVKYAVGYVIFPGGFGTMDELFEALTLIQTKKIRSFPVVL
                     VGRRYWEGLFDWLRNTMLAEKNISPEDLALMHVVDEPEEVCAIINNRYKERVTGRRED
                     RRDKAKKGV"
     gene            complement(1483..2220)
                     /locus_tag="VFK23_03530"
     CDS             complement(1483..2220)
                     /locus_tag="VFK23_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET7318189.1"
                     /translation="MITFGRKTIIGLVLTGLVLAGLLYLLYANGFIEYFTDRRRLLNL
                     INAHRAYAALIFIGLQALQVVVAPVPGEVSGFVGGVFFGTARGVLYSTVGLTLGSWMA
                     FMLARFAGRPLVELVVKTETIKRYDYVMKHKGMFLAFLMFLIPGFPKDILCYLLGLGH
                     MGQRDFLVVSTSGRLLGTVLLTLGGAFFRHRRYGALFTLAGVCIFIILLAMIYRETIE
                     RWFRSMRAAQLIKHRARSRAKKDKQAT"
     gene            complement(2238..2483)
                     /locus_tag="VFK23_03535"
     CDS             complement(2238..2483)
                     /locus_tag="VFK23_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318190.1"
                     /translation="MIDRSVGRREVCPSCGADMHCCLNCKFYDPTVSKQCRETAAELV
                     TEKARANYCEYFVFADRAGVNSNAEEARRALDHLFRK"
BASE COUNT          636 a          762 c          800 g          607 t
ORIGIN      
        1 tttccacggg catggtcggg cagctcggcc ttttgatcaa gaacacgctc ctcgccatat
       61 tgaacctttt tatcatgctg ttcgtcatct tctttttctt ccgcgatgga aaagcttacg
      121 cccgcgagct cacggacatc ctccccttgc caaggaacca caagaagtcc atcatggaaa
      181 agctcggcaa caccttttcc gcggtgctga acggtctgtt cctcgttgct ctcctgcagg
      241 gattcatgac cggcgtggga tttttcctct tcggggtccc ctacgggatc ttctggggat
      301 tcctggcgat gatcgcgtcg ctcctgcctt tcggtggaac ggccctcgtc tggattcccg
      361 gtgcgctcta cctcttcctc aagggatcga ccctcaaggc cgtcctgctt acggtgtggg
      421 gcgtgctcct cgtgagcacg cccgacaatt ttctgaagcc gatcctgatc ggcaggaagg
      481 caaaactgcc aacctttttc ctcttcctcg ggatcctcgg aggaattcac gtctacggaa
      541 tcctgggcat ccttttcggc cccatgatcg taacccttct gaccgccttc gtccagattt
      601 accgcgaaga gtatggggag cagcagggat aggggccagg agaggaaagc gccaaataac
      661 aagctcccaa taacaaataa gttccaagca tcaaattcaa aatttcaaac gtgcgaacaa
      721 tgacttttga atttggaatt tatttggaac ttgtgatttg cattttgaaa tttatgcttt
      781 tcggagagga gcagcaatgg aagacctggc aaaatccgat acctggcgtg tgttcagaat
      841 catggccgaa ctggtggagg gcttcgaggc cctgaacggc gtcggacccg ccgtgacgat
      901 tttcggaagc gcgcgtctcc cgacgggttc gccttactac aacaagtgcc tgaaggtggc
      961 cgagcgtctg gcgcaagacg gcttcgccgt catctcggga ggggggcccg gcatcatgga
     1021 ggcggcaaac aagggcgcgc agaacgcaca aggcgtctcc gtcggcctga acatcgatct
     1081 tccgctggag cagaccccga accagtttca ggacatccgg gtcgaattcc ggtacttttt
     1141 cgtacgaaag ctgatgttcg tgaaatacgc cgttgggtac gtcatattcc cggggggctt
     1201 tgggaccatg gacgaactct tcgaagcgct tacgcttatt cagaccaaaa aaatccggag
     1261 cttccccgtc gtgctggtcg gcagacgtta ttgggaagga ctgttcgatt ggctccgaaa
     1321 tacgatgctt gcggagaaga atatcagccc agaagacctc gccctcatgc acgtcgtgga
     1381 cgagcccgag gaggtctgcg ccatcatcaa caatcgatac aaggaacgcg tgaccggcag
     1441 gagggaagac cggcgggaca aggccaagaa aggagtatga aatcaggttg cctgcttgtc
     1501 ttttttggcg cgcgagcggg cccggtgttt tatgagctgg gcggcgcgca tactgcggaa
     1561 ccatcgctcg atggtctcac ggtagatcat ggcgagcaga atgatgaaga tgcagacgcc
     1621 ggcgagtgtg aagagggccc cgtaccgccg gtgacggaaa aacgctcccc cgagcgtcag
     1681 cagcacggtg ccgagcagcc ggccggaggt agaaacgacg agaaaatcgc gctgacccat
     1741 atggccgagt ccgaggaggt agcagagaat gtccttgggg aatcccggaa tgaggaacat
     1801 gagaaaggcg agaaacatgc ctttgtgctt catcacatag tcataacgtt tgatggtttc
     1861 agtttttacg acaagctcga caagcggacg gcctgcgaac cgggcaagca taaacgccat
     1921 ccacgagccc agggtaagtc cgacggtcga atagagtacg cccctggcgg tcccgaaaaa
     1981 aactcctccg acaaaaccgg acacctcccc gggaacgggc gccacgacga cctgcagcgc
     2041 ctgcagcccg atgaagatca aagccgcata tgctcgatgg gcgttgatga ggttcagcag
     2101 gcgtcgccgg tccgtgaaat attctatgaa tccgttggca tagagaagat acaggagacc
     2161 ggccagcacg aggccggtca gtacgaggcc gatgatggtt ttcctgccga atgtaatcat
     2221 tccgaaagcc gcacccttta tttcctgaag agatggtcga gcgcgcggcg cgcttcctcg
     2281 gcgttggaat tgacgcccgc tctgtccgcg aacacgaaat attcgcagta atttgccctt
     2341 gctttctccg tcacgagctc cgcggcggtc tcacggcatt gtttggaaac ggtgggatcg
     2401 tagaatttac agttgaggca gcagtgcata tcggccccgc aggaggggca tacttccctc
     2461 ctgccgacgc tccggtcgat caagagggct ttcttgcagg catggcaggc agtcacgatg
     2521 acgtataata gcgtatcagt ccgttttaga caagttgaaa aatggaacgg ctggctctct
     2581 tgactctccg gatagtttcg tataatatga accgttagct ttcgtggcct tcacccgacc
     2641 cttgcgaacc cgccgtcgtg cagtaataga gaagcgagag caagaattaa agtctggtct
     2701 tatggtgcgc atactgctca ttatcgggct gctggcgtcg ttctcagtac cgggccccgc
     2761 ggcgcagaaa aaagccggcg gctctgctga aacgctgcat caatt
//
LOCUS       DASSLD010000191         3371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1614372, whole genome shotgun sequence.
ACCESSION   DASSLD010000191 DASSLD010000000
VERSION     DASSLD010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614372"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFK23_03540"
     CDS             <1..205
                     /locus_tag="VFK23_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318191.1"
                     /translation="ERALDIGRDGAGEDVSLLNSEFSRINANEGRELLRIEDVPVKRK
                     KQSVVLVPFAQIRVHSRQNGISF"
     gene            189..1310
                     /gene="tgt"
                     /locus_tag="VFK23_03545"
     CDS             189..1310
                     /gene="tgt"
                     /locus_tag="VFK23_03545"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET7318192.1"
                     /translation="MAFRFDLIKKDKDSPARLGKFITPHGEVNTPVFMPVGTQATVKT
                     LSPDDLVSVGAEIILSNTYHLFLRPGHELIREFGGVQKFMGWNRPVLTDSGGFQVFSL
                     AELRKITEEGVMFQSPLDGGARHLITPEYAVEIQEALGADIIMAFDECIPYPATRDYA
                     LESLERTLRWAGRCRDAKKSADQALFGIVQGGMYPDLRRRSAEALVEIGFDGYGLGGL
                     SVGETKPMMYEMIEATAPALPEDQPRYLMGVGTPEDLVEGVDRGIDMFDCVMPTRNAR
                     NGTFFTSFGKVVIRNSRYERDKDPVDPVCGCYTCRNFSRAYLRHLFNAGEVLALRLGT
                     IHNLYFYLHLMRAVRASIEQRRFKEFKKEFLAKRESQDN"
     gene            1432..1767
                     /gene="yajC"
                     /locus_tag="VFK23_03550"
     CDS             1432..1767
                     /gene="yajC"
                     /locus_tag="VFK23_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET7318193.1"
                     /translation="MFISEAFAMGAPGGAGAQGGGGGFSSLIMMVVIFAIFYFILIRP
                     QQKKMKEHKKLVEELKKGDRIITSGGIYGTVENSTPETLTVKIAEGVKVKITRGSVAA
                     VVKPEEEKE"
     gene            1888..>3371
                     /gene="secD"
                     /locus_tag="VFK23_03555"
     CDS             1888..>3371
                     /gene="secD"
                     /locus_tag="VFK23_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET7318194.1"
                     /translation="MKKSIQWRLAVIALSVLIALFLFLPSTPLREKLPAVWSKSVPKI
                     SLGLDLRGGSHLVMQVETDKAVESSVDNIVADLQTTATQQGLAVNFSRSGQDVVAKFS
                     ETLRDKVEKHVKDNYGILDVKSSGNGELSYSLKPAEVKRLKETAVTQALERTRRRIDK
                     YGVAEPAIYKQENDQIALQLPGIKNPQEAISFIKTAGRLEFKLLNTRADIQKALAGEV
                     PPDSEILYGEDTNEQGKIVKRPYLLYKQTLLTGDRLKEAKVGIDQYNKPAVSISFDSK
                     GAEIFDRITGENVGNQLAIVLDGVVHSAPRIQDRITGGNAQITGNFTHDEAAKLAIVL
                     RESLPAPMKIIQNITVGPSLGQDSIDNGVRAALIGALVVIVFMVVYYRMSGVIADYAI
                     ILNLVLLLGAMALLNATLTLPGIAGVILTIGMGVDSNVLMFERIREEIRAGKQPRPAV
                     DSGYDKAFLTILDSHVTTLITAAVLFMFGTGPIKGFAVSLSLGI"
BASE COUNT          737 a          947 c         1035 g          652 t
ORIGIN      
        1 ggagcgcgct ctcgatattg ggcgtgacgg cgccggagag gatgtgagtc ttttgaacag
       61 cgaattttcg cgaattaacg cgaatgaggg ccgtgaatta ttaaggatag aagatgtccc
      121 ggtaaagaga aagaaacaat ccgttgtgct tgttccattc gcgcaaattc gcgtacattc
      181 gcggcaaaat ggcatttcgt tttgatctta taaagaagga taaagattct cccgcccggc
      241 tgggaaaatt catcacccct cacggtgagg tcaatacgcc ggtgttcatg ccggtgggga
      301 cgcaggccac ggtcaagacg ctctcacccg acgacctcgt cagcgtggga gcggagatca
      361 tcctgtcgaa cacctatcac ctgttcctgc ggccggggca cgaactgata cgcgagttcg
      421 gcggggtcca gaagttcatg ggctggaacc ggccggtcct gacggacagc gggggattcc
      481 aggtcttcag cctggcggaa ctccggaaga tcaccgaaga gggagtgatg tttcagtctc
      541 ctctcgacgg cggcgccagg cacctgataa cgcccgagta cgccgtggag atccaggagg
      601 cactcggcgc cgacattatc atggcctttg acgagtgcat tccttatccc gcgacacgcg
      661 attacgccct ggagtcgctg gagagaacgc tgcgttgggc cggacgctgc agggatgcga
      721 agaagtctgc ggaccaggcg ctgttcggga tcgttcaggg cggcatgtac ccggacctcc
      781 gaagacgctc cgcggaggcg cttgtcgaga tcggatttga cggctacggc ctcggcggcc
      841 tttcggtggg cgagacgaag ccaatgatgt acgagatgat cgaggcgacg gcgcccgcgc
      901 tgccggagga tcagccccgc tacctcatgg gagtcggcac acccgaggat cttgtggaag
      961 gcgttgaccg cggcatcgac atgttcgact gcgtgatgcc tacgaggaac gcccggaacg
     1021 ggacgttctt cacctccttc ggcaaggtcg tcatcaggaa ttcgcggtac gagagggaca
     1081 aggatccggt cgacccggtc tgcggctgtt acacctgcag gaacttctcg cgggcgtatc
     1141 ttaggcactt gttcaacgcc ggagaggttc tggcgctccg gcttggaacg atccacaacc
     1201 tttacttcta cctgcacctc atgcgcgccg tcagggcgag catcgagcag aggcggttca
     1261 aggagttcaa gaaggagttc ttggcaaaaa gagaatcgca ggataattaa ccacaaagac
     1321 atagagacag atcggggagt ctcccacttg gggatttttc tctgagcctc ggcgtctccg
     1381 tggtggattc atgagttttt agatccggta ctatttcaag gagggaacgc catgttcatc
     1441 tcagaagcat tcgcaatggg tgcgccgggg ggcgccggag cgcagggggg cgggggaggc
     1501 ttttcgagcc tcatcatgat ggtggtcatt ttcgccatct tctatttcat cctgatccgt
     1561 ccccagcaga agaaaatgaa ggaacacaag aagttggtgg aggaactcaa gaagggcgac
     1621 cgcatcatca cctcgggggg catctacggg accgtggaaa acagcacgcc tgaaaccctg
     1681 acggtcaaaa tcgccgaggg agtcaaggtg aagatcaccc gcggctctgt ggccgccgtg
     1741 gtgaaacccg aggaagagaa ggaataattc tggaaggtga gagggtagga aggtaagagg
     1801 gtaggagaac atcaacgctt ggtttctttt cctactttcc caccttccta ccttcctccc
     1861 ctcgtgattt tcagggaggc aagtatcatg aagaagagta tccagtggag actggcggtc
     1921 atcgccctgt cggtgttgat cgcgttgttt ttgttcctgc cgtccacacc gctcagggaa
     1981 aaacttccgg cagtgtggtc aaaaagcgtg cccaagattt cgctcggcct cgatctccgg
     2041 ggcggcagcc atctcgtaat gcaggtcgag acggacaaag cggtcgaatc gtccgtcgac
     2101 aacatcgtgg ccgatctgca gacgactgcg acgcagcagg ggcttgccgt taacttcagc
     2161 cggtcaggcc aggacgtcgt agccaagttc tccgaaacgc ttcgggacaa ggtcgagaaa
     2221 cacgtcaagg acaattacgg gatcctggac gtgaaatcgt ccggaaatgg tgagctttca
     2281 tacagcctta agcccgccga ggtcaagcgg ctcaaggaaa cggctgttac acaggcgctc
     2341 gagcgtacgc gccggaggat cgacaagtac ggagtcgccg agccggccat ctacaagcag
     2401 gaaaacgacc agatcgccct ccagctcccg gggatcaaga atccgcagga ggccatttcg
     2461 ttcatcaaga cggcggggag gctcgaattc aagctgttga atacccgggc ggacatccag
     2521 aaggcgctcg cgggcgaagt accgcccgac agcgagatcc tctacggcga ggacaccaac
     2581 gagcagggca agatcgtgaa gcggccatac ctcctgtaca agcagaccct cctgaccggt
     2641 gaccggctga aggaagccaa ggttgggatc gaccagtaca acaagccggc ggtctcgatc
     2701 tccttcgaca gcaagggcgc cgagatattc gaccggatca cgggagagaa cgtcggcaat
     2761 cagcttgcca tcgtgctgga cggcgttgtg cattccgcgc cccggatcca ggaccggatc
     2821 accggcggaa acgcccagat cacgggcaat ttcacccacg acgaggccgc gaaattggcc
     2881 atcgtgctga gggagtcgct tcccgctccc atgaagatca tccagaacat caccgtcggc
     2941 ccgtctttgg gccaggactc catcgacaac ggtgtccgcg ccgccctgat cggcgccctc
     3001 gtggtgatcg tcttcatggt cgtctattac aggatgtctg gtgtcatcgc tgattacgcc
     3061 atcatcctga acctggtcct cctgttgggc gccatggcgc tcctcaacgc gaccctcacc
     3121 ctgccgggca tcgcgggagt gattctcacc atcggcatgg gcgtggattc gaatgtgctg
     3181 atgttcgagc gcatccgcga ggagatccgc gccggcaaac agccgcggcc cgcggtcgac
     3241 tcggggtacg acaaggcgtt cctgacgatc cttgattccc atgtgacgac gctcatcacc
     3301 gcggccgtcc tgttcatgtt cggcacgggg cccatcaagg ggttcgccgt cagcctgagc
     3361 ctcggcatca t
//
LOCUS       DASSLD010000192         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_72462,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000192 DASSLD010000000
VERSION     DASSLD010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72462"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..972
                     /locus_tag="VFK23_03560"
     CDS             100..972
                     /locus_tag="VFK23_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HET7318195.1"
                     /translation="MFEEQITSLPDDVFRLLRDFIHGYCGIYFDDGSKYLLERRLARR
                     LELHRLKSFEEYYHFLRYDRKREEELTALIDNLTTNETYFFRENAQLHAFSEEILPDL
                     RERLAERKTLRIWSAGCSTGEEPYTIAILLLESGDWWRDWQVEIIGSDINQRVLHAAR
                     KGVYKKGAHRATDPGMLAKYFTLDEKGDYRVIDRVKELVSFSYVNLLDPHKTSLIGGM
                     DVIFCRNVIIYFDREAKKKVIESFYHKLRDGGYLLLGHSESLINLSNAFVLRTLKHDM
                     VYQKPLRCRTDQER"
     gene            989..2074
                     /locus_tag="VFK23_03565"
     CDS             989..2074
                     /locus_tag="VFK23_03565"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HET7318196.1"
                     /translation="MTERNRIKALVVDDSAYNRTVISKMLSSDPDIEVVGSAVDGVDA
                     IAKTLRFLPDVITLDLEMPNMDGFSFLRWLMQERPTPVIVLSARSDSRSVFRALELGA
                     VEFLAKPESRVSQSIETVRDDLLFKVHAVLNLQMSKVKSTIELLAKEHAAPVVPKEDE
                     ARREQGPVEVVAIASSTGGPPALQAILTALPGDLSAAVVIAQHMPPGFTRSFAERLNK
                     ISSLVVREAAAGDRVEPGTVLIAPGGHHLVVKKDKDGLLTGLTARSEHDMYSPSADRL
                     LVSVAQACGHAALGVVLTGMGKDGAAGVVAIKKNRGQCLAESEESAVIFGMPQEAIKT
                     GAVDKVLPLGAMAEEIKKRCAPKKKPA"
     gene            2114..2794
                     /locus_tag="VFK23_03570"
     CDS             2114..2794
                     /locus_tag="VFK23_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HET7318197.1"
                     /translation="MAGIKDIKALAFDAYGTIFDVFSVTALCEELFPGKGSALAGLWR
                     AKQLQYSLLRSLMGRHRDFWDLTEDGLVYATKSLHLDLTPSARTRLMESYLHLALFPD
                     VKPGMAALKKKGLLLSILSNGDPKMLEAVVDNSGISGLFDTIISAKEAGIYKPSPRVY
                     MLGPERMKVSSEQLGFVSSNSWDISGAASAGLPTFWIQRIENEPPEELGFSADHTVQS
                     IGELAQIV"
BASE COUNT          632 a          919 c          897 g          537 t
ORIGIN      
        1 gcgcggacgg gggcagtcga cgccgccctg gaaacgatcg ccgcagagga cccggatgcg
       61 gcggtgaggc aggcggccaa agaggccctg gggagataaa tgttcgaaga gcagattact
      121 tcgctgcccg atgacgtctt caggctcctc agggatttca tccacggcta ttgcgggata
      181 tattttgacg acgggtccaa gtacctgctc gaacgccggc ttgcgcggcg cctcgaactg
      241 caccggctca aaagcttcga ggagtactat cacttcctga ggtacgaccg gaagcgggaa
      301 gaggaactca ccgcgctcat cgacaacctc accacgaacg agacctattt tttccgcgag
      361 aacgcacagc tccatgcctt ttccgaggag atcctgcccg acctgcgcga gcgtctcgcc
      421 gagcgcaaga cgctccgcat ctggagcgcc gggtgctcaa ccggcgagga accttacacc
      481 atcgccatac tcctgctcga atcgggggac tggtggcgcg actggcaggt cgagatcatc
      541 gggagcgaca tcaaccagcg ggtgctgcac gcggcccgga agggggtcta caagaagggc
      601 gcgcaccgcg cgacggaccc cggcatgctg gccaagtact tcaccctcga cgagaagggc
      661 gattaccgcg tcatcgaccg cgtcaaggag ctcgtttcgt tcagctacgt aaacctgctc
      721 gatccccata agacaagcct gatcggcggc atggacgtca ttttctgcag gaacgtgatc
      781 atctatttcg accgcgaggc gaagaaaaag gtgatcgaga gcttctacca caagctccgg
      841 gacggcggtt acctgctcct gggacattcc gagtctctga tcaacctctc gaacgccttc
      901 gtactccgca cgctcaagca cgacatggtt taccagaagc cgctccggtg caggacagac
      961 caggaacgat gattccgcag ggggaagcat gacagaaagg aacagaatca aggccctcgt
     1021 cgtcgacgat tccgcctaca accgcacggt gatctccaag atgctttcga gcgacccgga
     1081 tatcgaggtc gtcggcagcg cggtcgacgg cgtcgacgcc attgccaaga cgctccgctt
     1141 cctgcccgac gtgatcaccc tggacctcga gatgccgaac atggacggct tctccttcct
     1201 ccggtggctc atgcaggagc gtccgacgcc cgtgatagtc ctctcggccc gctccgacag
     1261 caggagcgtg ttccgcgccc tcgagctcgg cgccgtggag ttccttgcca agcccgagtc
     1321 ccgcgtgtcc cagtcgatcg agaccgttcg ggacgacctc ctcttcaagg tccacgccgt
     1381 cctgaacctc cagatgtcca aggtcaagtc gacgattgag ctcctggcga aggagcatgc
     1441 ggcgcccgta gtcccgaaag aggacgaggc gcggcgggag caggggccgg tcgaggtggt
     1501 ggccattgct tcgtccacgg gcgggccgcc ggccctgcag gccatcctga ccgccctccc
     1561 aggcgacctc tccgccgccg tcgtcatcgc gcagcacatg cctcccgggt tcacgcggag
     1621 cttcgccgag cggctgaaca agatttcgtc cctcgtcgtt cgcgaggcgg cggcggggga
     1681 ccgcgtggag ccgggcaccg tgctgatcgc gccgggaggg catcatctcg tcgtgaagaa
     1741 ggataaggac ggcctcctga cgggcctcac tgcccgcagc gaacacgaca tgtactcgcc
     1801 gtccgcggac cggctgctgg tctccgtggc ccaggcctgc ggccacgcag cgctcggggt
     1861 ggtgctgacc ggcatgggca aggacggcgc cgcgggggtg gtcgccatca agaagaatcg
     1921 gggccagtgc ctggccgaga gcgaggaaag cgccgtcatc ttcggcatgc cccaggaggc
     1981 catcaagacg ggcgccgtgg acaaggttct gccgctgggc gccatggccg aagagatcaa
     2041 gaagcgctgc gcgccgaaaa aaaagcctgc gtgagctgtt ttaaatatgg tcacggtaaa
     2101 aaagcagtgc tcaatggccg gaataaaaga tataaaggcg ttagcgttcg atgcctatgg
     2161 aacgatcttt gacgttttct cggtgaccgc gttgtgcgag gagctgtttc ccggcaaagg
     2221 gagcgcgctg gccgggctct ggcgcgcaaa gcagctccag tacagcctgc tccgaagcct
     2281 gatgggccgc caccgggact tttgggactt gaccgaggac ggcctcgtct acgcgacaaa
     2341 aagtcttcat ctcgatttga ctccgtcggc acgcacccgc ttgatggagt cttaccttca
     2401 cctcgccctc tttcctgacg taaagccggg tatggcggcg ctgaagaaaa aaggtctgct
     2461 cctgtccatc ctgtcaaacg gagatccaaa gatgctggag gctgtcgtag acaacagcgg
     2521 gatctcagga ctcttcgaca ccattatcag tgccaaagaa gcaggaatct acaagccgag
     2581 cccgcgggta tacatgctcg gccccgaacg catgaaggtg agcagcgaac agctaggatt
     2641 cgtttcctcg aactcatggg atattagcgg cgcggcatca gccggcctgc ccacgttctg
     2701 gatccagcgc attgagaacg agccgcccga ggaactcgga ttttcagccg atcacacggt
     2761 gcaaagcatc ggtgaactcg cgcagatcgt gtgaaaacat atcggaggcg catcataggg
     2821 ggaaggcgac tttgtgcctc ttttcagttt caagctaatg acctgcatcc tcgacatcat
     2881 cctgaactat tggagttccc atacttcgca acaatcacaa cttcatctgg aattaactaa
     2941 ctgatacccc acaaataccc ctttccggtg attgacaaca acgtc
//
LOCUS       DASSLD010000193         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_85926,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000193 DASSLD010000000
VERSION     DASSLD010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85926"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFK23_03575"
     CDS             complement(<1..253)
                     /locus_tag="VFK23_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HET7318198.1"
                     /translation="MAEMIEIRWHGRGGQGTVTAAKVLADACLSGGRNVQAFPEYGPE
                     RAGAPLRAYNRISEKALRMHCPVLNPGVVAVVDATLIDAI"
     gene            complement(343..1041)
                     /gene="recR"
                     /locus_tag="VFK23_03580"
     CDS             complement(343..1041)
                     /gene="recR"
                     /locus_tag="VFK23_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET7318199.1"
                     /translation="MDSKSEIDMKKEQDTTSQVSVRSRRRENAGDQDGKISGKALGRM
                     VEAFMKLPGIGRKSAERLAFAVLGMSQAEAAGIAEAILDLKNNSRYCTVCNNITEEEP
                     CGICRDEKRDPSRICVVEEPSNILILEKTGSFRGRYHALLGAISPLDGVGPEDLRIDG
                     LMVRLRSGAVKEVIIATNPTVKGETTALYLSKLIKPLGISVSRIAYGLPVGGDIEYAD
                     ENTVQRALEGRREM"
     gene            complement(1056..1376)
                     /locus_tag="VFK23_03585"
     CDS             complement(1056..1376)
                     /locus_tag="VFK23_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET7318200.1"
                     /translation="MSKNMLSNIMKQAQQMQERVKKLQEEAGGKTVEASSGGGMVTVV
                     ANGRQEVLSIKIDPSVVDPKDNEMLQDLVTAAVNEALRKSQDLMKEEMGKLTAGMGLP
                     PGMF"
     gene            complement(1595..>2056)
                     /locus_tag="VFK23_03590"
     CDS             complement(1595..>2056)
                     /locus_tag="VFK23_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318201.1"
                     /translation="SPHGISEQRATYVPAPKTAASQPRAVSTGRGEHADPWSGILSVL
                     KSRKPGLASALGHSRVIDQTDTDLVIGVHGTAFQVKKAEEPDSRNLIEQVAEEVLGRR
                     VRVKIQPVEAPAKPASAAKDPKKAIPDEHDALAQDVAKLFGGEVIERDREQ"
BASE COUNT          347 a          643 c          569 g          497 t
ORIGIN      
        1 tgatcgcatc aatgagcgtt gcgtccacga ccgccaccac gccggggttc agcacggggc
       61 aatgcatgcg gagcgccttc tcgctgatgc ggttataagc gcggagcgga gctcccgctc
      121 gctcgggtcc gtactccgga aaggcctgca cgttccgccc gccggaaagg cacgcgtccg
      181 cgagcacctt ggcagcggtc acggtcccct gtccgcctct tccatgccat ctgatctcaa
      241 tcatttctgc catacggtta cctccacaaa tcaagatcga cacgcgatac aacgcgggcg
      301 gcttttcggt tttcattccg cattccgcac tccgcattga acttacattt cccttcgtcc
      361 ttcgagcgcc cgctggaccg tgttttcatc ggcgtactcg atgtccccgc ccacgggaag
      421 gccgtaggct atgcgggaga cgctgatgcc gaggggtttg atcagtttcg aaagatacag
      481 ggccgtggtt tctcccttga ccgtcgggtt cgtcgcgatg atgacttcct tgaccgcgcc
      541 ggaccggagc cgcaccatga ggccgtcgat ccttaaatct tccgggccca cgccgtccaa
      601 cggagagatg gcgcccagga gcgcatgata acggccgcgg aagcttccgg tcttttcgag
      661 gatcaggatg ttgctcggct cttctacgac gcagatccgg ctcgggtccc gcttctcgtc
      721 gcggcagata ccgcagggct cctcttcggt gatgttgttg cagacggtgc agtaacggct
      781 gttgttcttg aggtccagga tggcctcggc gatccccgct gcctcggcct gcgacatgcc
      841 caggacggca aacgcgaggc gctcggcgct ttttctgccg atgccgggaa gcttcatgaa
      901 ggcttcgacc atccggccca gggccttgcc ggatattttc ccgtcctggt ccccggcatt
      961 ctcccgtctt ctacttctca ccgacacctg cgacgtcgtg tcttgttctt tcttcatatc
     1021 aatttcggat ttgctgtcca tgccgcaatc cgaaatcaga acatccccgg cgggagcccc
     1081 atgccggcgg taagtttgcc catttcttcc ttcatgagat cctgcgattt tctgagggct
     1141 tcgttcaccg ctgcagtgac caggtcctgg agcatctcgt tgtccttcgg gtcgaccacc
     1201 gaggggtcga tcttgatcga caggacttcc tgcctcccgt tggcgacgac ggtcaccatc
     1261 cctccgcctg aagaggcttc gacggttttc cctcccgctt cttcctggag ttttttgacc
     1321 cgctcctgca tctgctgggc ctgcttcatg atgttgctga gcatgttttt cgacatggga
     1381 aatcctggac aaacgagatt ataaaactgc taggaaagca tataataaaa gaattgaaac
     1441 ggttttgcaa caaaaaatgc ggggtttatg gatacataaa aaaaacaaat gtggcctgct
     1501 gcgtcaagcc ggatcgtaac cgcatgcccc tgcacgtcgc ttctgaagcc gcttcagaag
     1561 gccatcggct tattgctttt ttctcctcgg ccggtcactg ctccctgtct cgttcgatca
     1621 cctcgccgcc gaacaatttc gcgacgtcct gggccagggc atcatgttcg tcaggtattg
     1681 cttttttggg gtccttcgca gctgatgccg gtttcgccgg cgcttcaacg ggctggatct
     1741 tcacccggac ccgccgcccc agaacttctt cagcgacctg ctcgatcaga ttgcggctgt
     1801 cgggttcctc ggccttcttt acctggaatg cggtgccgtg cactccgatc accaggtctg
     1861 tatcggtctg atcaatcacc ctgctgtgac cgagcgcgga ggcgaggccc ggctttcgtg
     1921 atttcagcac ggacagtatc ccgctccagg ggtcggcgtg ttccccccgg cctgtcgaaa
     1981 cggcacgggg ctgcgatgcg gccgttttcg gagcagggac gtaggttgcg cgctgttcgc
     2041 ttatcccgtg cggtga
//
LOCUS       DASSLD010000194         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1605075, whole genome shotgun sequence.
ACCESSION   DASSLD010000194 DASSLD010000000
VERSION     DASSLD010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605075"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VFK23_03595"
     CDS             <1..349
                     /locus_tag="VFK23_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7318202.1"
                     /translation="MKQRCGQIISIGSIVGIQGREGQANYAASKAGLMGLTRACAREL
                     GPYNIRVNAVLPGYLLTDMGRKASNAVLERVRRENALGRPSDPEEVAEFVYRLSLMEN
                     VSGQIFNLDSRII"
     gene            467..1132
                     /gene="bioD"
                     /locus_tag="VFK23_03600"
     CDS             467..1132
                     /gene="bioD"
                     /locus_tag="VFK23_03600"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HET7318203.1"
                     /translation="MNKGIFITATDTGVGKTYIASGIAAELLRRGVDVGVMKPAETGC
                     SMRGGRLVPLDAKHLMKATGVNDPIALVNPYRFHRPLAPSVAARLSGKKIEPDKIISA
                     YRDLSRRHDFMIVEGAGGIMVPLRGAYTFLDLAQELAIPVLVVARPGLGTINHTLLTV
                     AALGQRRLRISGIVINYAVKQKSGLAENTNPEVIQRISGLKIVGTIPFGSYQFHDLVD
                     RLV"
     gene            1416..>2551
                     /locus_tag="VFK23_03605"
     CDS             1416..>2551
                     /locus_tag="VFK23_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318204.1"
                     /translation="MKKSFGALSAAAVLMLGLAVFFASCGGGGGGGSSAPTITPISTP
                     AQGAQSATAAVSTFRSMSNAGSSVTGMAGLGGLSLAPQIKMPVSTGSASTAAGKFAVR
                     FATVVKKAEAMRVSASGYPMPMTCSSGSGTIDMDASGTSMIMTSNNCRNGDIMTDGIM
                     SVSGISQTSNSGSGNITVGSDADPFIMTVYSGATSTVIDEVTTAVVGMTFTASGTSTA
                     DMSFSMTMNGYFDVVDHVTHTHERQVVNNLSLGGGTGSTTTIGSASYDVATLTLNGTM
                     TGTTYISDTDPAVSYQEGPNTFWNVVIVDKTPVFGTGDEYFSIVGTITVSVTPAKCID
                     GTFSITTDTAVQIDGVTGQTKAGRMTINGNVVATFNSDGSISVS"
BASE COUNT          628 a          669 c          703 g          551 t
ORIGIN      
        1 gatgaagcag cgttgcggac agatcatctc gatcggctcc atagtcggga tccaggggag
       61 ggaaggccag gcgaattacg cggcgtcaaa ggcgggtctc atgggattga caagggcctg
      121 cgcacgggaa ctcggtcctt ataatatcag ggtgaacgcg gtcctgcccg gttacctctt
      181 gaccgacatg ggaaggaagg cgtcaaatgc cgtccttgag cgggtccggc gcgagaacgc
      241 actgggacgg ccgtcggatc ctgaagaggt agccgagttt gtgtatcgtc tttccctgat
      301 ggagaacgtt tccgggcaga tatttaattt ggacagcagg atcatctgag cgaccgtgtc
      361 atgcccgaaa ggttctttcc cgatagattc attcgaggaa gaatacgatt cataaaacca
      421 taccccccga tggaaacatt cggggaagac ggaacaggat cagctcgtga acaaaggcat
      481 tttcataacc gcgaccgata cgggcgtggg gaagacgtat attgcgtcag gaattgccgc
      541 cgaactgctg cgtcgcggag tggatgtcgg cgtcatgaag cccgccgaga ccggctgcag
      601 catgcgcggc ggacggctcg tcccgctgga tgcgaaacac ctcatgaagg cgactggcgt
      661 caacgatcct atcgccctcg tgaatcctta ccgctttcac agacccctcg ccccttcagt
      721 ggcggctagg ttatccggga aaaagattga acccgataaa atcatctctg cctacaggga
      781 cctttcccgc aggcacgatt ttatgatcgt tgaaggagcc ggcggcatta tggtccccct
      841 gcggggagca tatacattcc ttgaccttgc acaggagttg gcgatccccg tgctcgtcgt
      901 ggccagaccg ggcctcggaa cgatcaacca cacgctgttg accgtagccg cactcgggca
      961 acggagactc aggatatcgg gcatcgtaat caattatgcg gtgaaacaga agtccggcct
     1021 ggcggaaaat acgaatcctg aggtgatcca aagaatttcc ggtttgaaga tcgtggggac
     1081 catcccgttc ggttcgtatc agttccatga tttagttgat cgtctcgtat aaagaccttc
     1141 cacgtcgact taaacctttt ttccctgact tctattaatc aaaaaatcaa taagttatca
     1201 gtgattattt catcttgcgc ttgaaaatac gcatcctttc tgcaatcaat gctgtaaaac
     1261 tcaataactt attaaataaa aaggtaataa tatatgttcg actttaattc ttcgactgtg
     1321 aggttaatca cagaaatata tctacaacat tagtattata aataggatat gaattcggca
     1381 aaacatacgc ttaaaaaata cacaaggagg gctcaatgaa gaaaagtttc ggtgcattat
     1441 cagcagcggc agtattgatg ctcggtcttg cggtgttttt cgcttcctgc ggaggcggcg
     1501 gtggcggagg cagcagcgca ccaaccatca cccctatcag cacgcccgcc caaggggctc
     1561 aatcggccac ggcagcggtc agcacctttc gttccatgtc gaatgcgggc tcatctgtga
     1621 caggcatggc gggcctggga ggcctttcgc tggcgcctca aataaagatg ccggtttcca
     1681 ccggttcagc gtcgactgca gctggcaaat tcgcggtgcg tttcgccact gtagtgaaaa
     1741 aagccgaggc catgcgagtc tcggcctcgg gatacccgat gccgatgacc tgtagctccg
     1801 gcagcgggac aatcgacatg gacgcctcgg ggacgtcgat gatcatgacc tccaataact
     1861 gcaggaacgg ggacatcatg accgacggta tcatgtccgt gagcgggatt tctcaaacct
     1921 caaattcagg aagcgggaac attactgtcg gatcggatgc cgatccgttt atcatgactg
     1981 tctactcggg cgccacgtcc acggtgattg acgaagtgac cacggctgtt gtgggcatga
     2041 ccttcacagc atcaggcact tccacggcag acatgtcgtt ttcgatgacc atgaacgggt
     2101 acttcgatgt tgttgatcat gtcacccata cgcacgaaag acaggtcgtg aacaacctct
     2161 cgctcggcgg cggcacgggc tccacgacga cgattggcag tgcgtcctat gatgtggcta
     2221 cactgaccct gaacggcaca atgacgggaa cgacctacat atcggacacg gacccggccg
     2281 tcagctacca ggaagggccg aatacgttct ggaacgtcgt gatcgtggat aaaactccgg
     2341 tgttcggcac gggagatgaa tatttcagca tcgtaggcac gataacggtc tccgtgaccc
     2401 cggccaagtg catcgacggc acgttctcga tcaccacaga cacggccgta cagatcgacg
     2461 gagtgacagg ccagaccaag gccggcagga tgacgatcaa cggaaacgtg gtcgcaacgt
     2521 tcaacagcga cggaagcatt tcggtctcca t
//
LOCUS       DASSLD010000195         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1523055, whole genome shotgun sequence.
ACCESSION   DASSLD010000195 DASSLD010000000
VERSION     DASSLD010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523055"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1512
                     /locus_tag="VFK23_03610"
     CDS             232..1512
                     /locus_tag="VFK23_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318205.1"
                     /translation="MSVVTLFGCSIIAQAEVPAPSPDWLTKNIRVLTMFDYQDPASST
                     QNPDNAFARLYRYSGEVDLRPDLIVDTPIVGWSFKPRVTYFYRWWEDGVTKGERDDEA
                     RFFVNEWMIQPKPTDNLFLSFGKEKLLWGNSFLVSPSNILFRDTEKVNPKAEVEGKYL
                     AQVTWLPSSSVTVNLIGRTQKEENALGESAQPVRSVKVDVVGGSYLVSLINYFQQHER
                     FRLGSYGQWTASDAVVLYYDGIVSKGTDVLYPVVDPGLQLSGEFQRTNAGSGRLYPAV
                     TAGGSYTFLSGETLSLEFLYNGAGYNDDEADAYYQLRQNAALHFLDSSPIGALSQKTL
                     VQSLFTGSDLLRRYYLMAQLQSREIKNVLNIIVRYTHGLEEHAGQTSSIIEWKMTDRL
                     EFFNINTVAVSRGRDTEFNALLDKSFLAGVEAHF"
     gene            1722..>2441
                     /locus_tag="VFK23_03615"
     CDS             1722..>2441
                     /locus_tag="VFK23_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7318206.1"
                     /translation="MAEPNKIIYSMVGVGKFYDKKPVLKDIYLSYFYGAKIGVLGLNG
                     SGKSSLLKIMASVDKEHVGEAVLSPGYTVGLLEQEPKLDPEKTVRQCVEEGVQEVVDL
                     LAEYNKINESFANPMSDDEMQKLIERQGEVQEKLDHANAWELDSRLEMAMDALRCPAG
                     DTPVKVLSGGEKRRVALCRLLLQKPDILLLDEPTNHLDAESVAWLEHHLQRYEGTVIA
                     VTHDRYFLDNVAGWILELDRGQ"
BASE COUNT          593 a          658 c          684 g          506 t
ORIGIN      
        1 tccgtgtgca gccgattccg gattcgacgt ttaatctgag tgtgctggtg agatgaattc
       61 ggaatgcgga atatgacaac ttcgtaaaag ccacggatcg tcattctgag cgcagcgaag
      121 aatctggcct tttcaggcca accagtccag acccttcgct tcgctcaggg tgacaggtta
      181 gggaatatta cgactttatc aaatacaacc atgagacatc cggcattcat agtgagcgtt
      241 gtaacccttt tcggctgttc aattattgcg caggcagaag tacccgcccc atcccccgac
      301 tggttgacga agaacatacg cgtactgacc atgttcgact accaggaccc cgcaagttcc
      361 acgcagaacc cggacaatgc ctttgcgcgg ctctaccggt attcgggcga agtcgacctg
      421 aggccggatc tgatcgtgga cacgcccatc gtaggctggt cgttcaagcc gcgtgtcact
      481 tacttttacc ggtggtggga ggacggcgta accaagggag aacgagacga tgaggccagg
      541 ttcttcgtga acgagtggat gattcagccc aagcccactg ataatctctt tctctccttt
      601 ggaaaggaga agctgctttg gggcaattca tttctcgtga gcccctccaa catccttttc
      661 cgggacacgg agaaggtgaa tcccaaggca gaggtggagg gaaagtatct ggctcaagtg
      721 acgtggctac cgagcagcag tgttacggtc aacctgatcg gccgcacgca aaaagaggaa
      781 aacgctttgg gagaatcggc gcagcccgtc cgctcggtca aggtcgatgt ggtcggcggg
      841 agttacctgg tgagcctaat aaactatttt cagcagcacg aacggttccg gctgggaagc
      901 tacggacagt ggacagcgtc ggacgccgtg gtcctgtact acgacgggat cgtgagcaag
      961 ggaacggatg tgctctatcc tgtcgtcgat ccgggccttc agctttcagg ggaatttcaa
     1021 agaaccaatg ccggctccgg caggctttat ccagcggtca cggcaggggg gagctacaca
     1081 tttctttcag gagaaacatt aagcctggaa ttcctttaca acggcgccgg gtataatgac
     1141 gatgaggcag atgcctatta tcaactcagg cagaacgctg ccttgcactt cttagacagc
     1201 agcccaatcg gggcgctctc tcagaagacc ctggtccaga gccttttcac cgggtccgat
     1261 ctcctgcgaa gatactatct catggcccag ctccagagca gggagataaa gaacgtcctc
     1321 aacatcatcg tccgatacac gcacgggctt gaggagcacg caggccagac gtcgtcgatc
     1381 atcgagtgga agatgacgga ccggctggag ttcttcaata tcaataccgt tgcggtaagc
     1441 aggggaaggg acaccgagtt caacgccctt ctcgacaaga gttttctcgc gggcgtggaa
     1501 gcgcatttct gagaaatctt cccgaaccag aaaagcctga acaaaaaagc ggtcccgcgc
     1561 agagcacgca gaagacatga aataaaatta tcgtgcaaac gattttctcc gcgcctcagc
     1621 gtcacgcgcg agctgttcct gccttgtttt tatcattgac ataaagctgt catacagtgt
     1681 acaaataagc ctcacaaatt cacggaggtc actggaacgc tatggctgaa ccgaacaaga
     1741 tcatctactc catggttgga gtgggaaaat tctacgacaa aaagccggtc ctgaaggaca
     1801 tctacctctc ctatttctac ggagcaaaga tcggggtgct gggtctgaac ggctcgggca
     1861 agagctcgct cctcaagatc atggcgagcg tggacaagga gcacgttggc gaggcggtgc
     1921 tctcgccggg ctatacggta ggacttctcg agcaggagcc gaagctcgat ccggaaaaga
     1981 ccgttcgtca atgcgttgaa gagggtgtgc aggaagtcgt cgatcttctt gccgaataca
     2041 acaagataaa cgaatccttc gccaatccca tgtccgacga cgagatgcag aagctcatcg
     2101 agcgccaggg cgaagtacag gaaaagctcg accacgcgaa cgcctgggag ctggattccc
     2161 gactcgagat ggccatggac gctcttcgct gcccggcagg cgataccccg gtcaaggtgc
     2221 tctcgggcgg cgaaaagagg cgcgtggccc tctgccgcct gcttcttcag aaacccgaca
     2281 tcctgcttct cgacgaaccc acgaaccatc ttgacgccga gtcagttgcc tggctggagc
     2341 atcatctgca gcgatacgag ggcacggtca tcgccgtgac ccacgaccgt tacttcctcg
     2401 ataatgttgc gggctggatt ctggaactcg accggggcca g
//
LOCUS       DASSLD010000196         3498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_562102,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000196 DASSLD010000000
VERSION     DASSLD010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562102"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VFK23_03620"
     CDS             complement(<1..475)
                     /locus_tag="VFK23_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET7318207.1"
                     /translation="MEYRSQKIAYWYFAVALLLFALQVVLGLWLAFNYSFTGPQSIVD
                     VFPFATARAMHTNLLVLWMLLGFMGGTYYIVPEETKSEIYSSGLAWYQLVALVATGVV
                     ALVGFIFGWTQGRPLLEIPTSLDFVVVVGALIFLFNVGMTMFKANRWTAIQGTLLG"
     gene            complement(485..1186)
                     /locus_tag="VFK23_03625"
     CDS             complement(485..1186)
                     /locus_tag="VFK23_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7318208.1"
                     /translation="MSNKTAFWIFLIGTVSSAILFLGLTWDTHRQVETLSHVDKLSDK
                     VVEGKRIFEKYNCNDCHTILGFGGYYAPDLTKVVQRVGEDGIRYRVKTPEKAFAASWR
                     KMPNEHVSDAEIDSLIAFFTWVGNVENGDWPPQDSKKRLSRSEERMVAGALASPGAAV
                     FQTRGCMNCHLLHGSGGTFGPALDTIGRTLGVEQIEHYIKDPKAVNPKAMMPPQTALS
                     EKEIEEVAAFLANLK"
     gene            complement(1272..1442)
                     /locus_tag="VFK23_03630"
     CDS             complement(1272..1442)
                     /locus_tag="VFK23_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318209.1"
                     /translation="MPSIFFSGYLSVETNAAATAQIPLTATLINRFFDIRHNVPLPAW
                     KERFMNEVSFGA"
     gene            1882..>3498
                     /locus_tag="VFK23_03635"
     CDS             1882..>3498
                     /locus_tag="VFK23_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7318210.1"
                     /translation="MAITRRGFMKICALGATAAAGSRFFKLAQSDEGVLHPGADVLEY
                     VRSTCSPNCTGACGFNAMVYKGRIQSLVQAADYPDTEYNPRGCLRGQSSLNLIYGPDR
                     LKQPLIRVGKRGEGKFKAVSWDEALDHVADRLAEIMQKYGPESVVGNIQVPGTGYVNK
                     GALMRLASMYDWSIIHAYTMNGDLPAFWPMTFGVQTEELESLEWTNSKYTLILGSNVM
                     VTRVPDAKFLNISRENGSKVVVVDPNYSPTASKADEWIQINPSTDAAFALGVVNVILN
                     EKLYDAEFIKTNTDMPLLVRLDNNKKLLAADVASLSARAGEQKKKIPEYRDLYVVYNK
                     NKKGFAIPDPESLKRDFDPALEGEFEVQLTDGRRVKVKPVFQLLSELMNKAYTPEKAA
                     RMIAPEESRVADYTEMIKRTARELATTRPLHIVYGASNYQWYHGDLKGRALSLIVTLT
                     GNLGKPGAGISTYAGQYRVRWPLGAWWGFKDKKNKWTNYLLWMNDEYRGGEEFKKYNK
                     EIPYPKNGVKALFYGWGNPFDQHNMANRMRE"
BASE COUNT          818 a         1001 c          986 g          693 t
ORIGIN      
        1 cgcccaggag cgttccctgg atcgcggtcc agcggttcgc tttgaacatg gtcatgccca
       61 cgttaaagag aaatataagg gcccctacga ccacgacgaa atcgagcgag gtcggtatct
      121 ccaggagcgg ccttccctgc gtccagccga agatgaaccc gacgagggcg acaacgccgg
      181 tagccaccag ggcgacgagc tggtaccagg cgagacctga ggaatagatc tcgctcttgg
      241 tctcttcagg cacgatgtag tacgtccccc ccatgaaacc gagcagcatc caaaggacaa
      301 gcaaattcgt gtgcatggcg cgagccgtgg caaaggggaa cacgtcaacg atgctttgcg
      361 gacccgtgaa gctgtaattg aaagcaagcc agagccccag gaccacctgg agcgcgaaca
      421 ggagcagggc aacggcaaaa taccagtagg ctatcttctg agagcggtat tccatcgtat
      481 cctcctattt taaattcgcc aggaacgcgg cgacttcctc gatttccttt tccgaaagcg
      541 ccgtctgggg cggcatcatg gctttcgggt tcaccgcctt ggggtctttg atgtaatgtt
      601 cgatctgctc gacgccgagt gtccggccga ttgtgtccag ggcaggaccg aaggtccctc
      661 cggagccgtg aagtagatgg cagttcatgc atccccgggt ctggaacaca gccgcgcccg
      721 gcgacgcaag cgctcccgcg accattctct cttcgcttcg cgaaagtcgc tttttgctgt
      781 cctggggcgg ccagtcgccg ttctcaacat tgcccaccca ggtgaagaac gcgatcaggc
      841 tgtcgatctc cgcgtccgaa acatgctcgt tcggcatctt gcgccacgac gctgcaaagg
      901 ccttttccgg agtcttcaca cggtaccgga ttccatcctc cccgacgcgc tggacgacct
      961 tcgtgagatc aggcgcgtaa taaccgccga acccgaggat cgtgtggcag tcgttgcagt
     1021 tgtacttctc gaatatccgc ttcccttcta caaccttgtc cgaaagcttg tccacatggg
     1081 aaagcgtttc gacctggcga tgcgtgtccc acgtgaggcc gagaaacaaa attgccgagg
     1141 acaccgtgcc gatcaggaaa atccagaaag cagtcttgtt tgacatcccg cacctccttc
     1201 tccgagaata gagtacgagc aatcataacg tatccgacca ccgataaagt atatcacagg
     1261 cacattgcgt gtcaggcccc gaacgagacc tcgttcatga acctttcttt ccaggccggg
     1321 agagggacgt tatgtcgaat atcgaaaaaa cggttgatca gcgtcgctgt gagcggaatc
     1381 tgcgcggtcg cggcggcatt ggtttcgacg ctgaggtacc cgctgaaaaa tatcgacggc
     1441 attctgacac cggcagctgt accacgcctt gacccatgtt cctgtcagtg tgcattatgc
     1501 aattattttc acaagaaata accaaaacgc atcagtgtgc agctttggtc ggcatgcacg
     1561 aaagccgttt tcgaccgtgc tatagttata aaaagagatc atttgcgaac tgtcatttga
     1621 tccgatcact gaccatcggt cacgcatcat tgcgcgcttc ttcctcgaac ggaagagggg
     1681 agacctccga cgatggacgg taattcctcg aaaaatattc acccgtcaga attcctgaac
     1741 tgtcaccctt gcggcatcgg acgtcgatgc gaaactcgaa cagggtacga cgccggtcgc
     1801 cgctgtcatc acatttcgat cgattctcat tatgcctgcg gaagattatt ctgcaggcgg
     1861 tccgtgtaaa ggaggtcgcg catggctatt acacgaagag gcttcatgaa gatctgcgcc
     1921 ctgggggcca cggccgcagc aggatcccga ttcttcaagc tggcccaatc tgacgaagga
     1981 gtattgcatc cgggagcgga cgtactcgaa tacgtgaggt cgacctgttc ccccaactgc
     2041 acaggcgcct gcggcttcaa tgccatggtc tataagggca ggatccagtc gctcgtgcag
     2101 gccgcagatt atccggacac ggagtataac ccgagggggt gcctccgggg gcagtcttcc
     2161 ctcaatctca tctatggtcc ggaccggctc aaacagccgc tcatcagggt cggcaagcgg
     2221 ggagagggca agttcaaggc agtgagctgg gatgaggccc tggatcatgt tgcggacagg
     2281 ctcgccgaga ttatgcagaa gtacgggccc gaatccgtgg tgggcaatat ccaggtcccg
     2341 ggaacgggat acgtgaataa aggggccctg atgcgccttg cctccatgta tgactggagc
     2401 atcattcatg cctacaccat gaacggcgat ctgccggctt tctggcccat gaccttcggc
     2461 gtgcagaccg aggaattgga gtccctcgaa tggaccaatt cgaaatatac cctgatcctc
     2521 ggttccaatg tgatggtcac gagagtgccg gacgcgaagt tcctgaacat cagcagggaa
     2581 aacgggtcca aggtcgtcgt cgtcgatccg aattacagcc ccacggcatc caaggccgat
     2641 gagtggatcc agatcaatcc gtccacggac gcagcctttg ctctgggcgt cgtgaacgtc
     2701 atcctgaacg aaaaactcta cgatgcggaa ttcatcaaga ccaacaccga tatgcccctc
     2761 ttggtccggc tcgacaacaa caagaagctt ctggccgcag acgtggcatc cctctcggcc
     2821 agggcggggg agcagaagaa aaagatcccc gaataccgcg acctttacgt tgtctataac
     2881 aagaacaaga aagggtttgc gattcccgat cccgagagcc tgaagcggga cttcgatcct
     2941 gcgctcgaag gggagttcga ggttcagctc acggacggcc gtcgggtgaa ggtcaagccg
     3001 gtgttccagc ttctgtcaga gttgatgaac aaggcgtaca ctcccgagaa ggcagcccgc
     3061 atgatcgctc ccgaggagtc gcgggtcgcg gactacacgg agatgatcaa aaggaccgcc
     3121 cgtgaactgg ccacgaccag gccgctccac atcgtgtacg gcgccagcaa ttaccagtgg
     3181 taccacgggg acctgaaggg cagggcgctc tcgctcatcg ttaccctgac cggaaacctc
     3241 ggcaagcccg gcgccggcat ctccacctac gcgggacagt accgcgtccg ctggcccttg
     3301 ggggcctggt ggggcttcaa ggacaagaag aacaaatgga cgaattacct gctctggatg
     3361 aatgacgagt accggggcgg cgaggagttc aagaaataca acaaagagat accctacccc
     3421 aagaacggcg tcaaggcttt gttctacggg tggggcaacc ccttcgacca gcacaacatg
     3481 gctaatagaa tgcgagaa
//
LOCUS       DASSLD010000197         4026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_495597,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000197 DASSLD010000000
VERSION     DASSLD010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495597"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2287)
                     /locus_tag="VFK23_03640"
     CDS             complement(<1..2287)
                     /locus_tag="VFK23_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7318211.1"
                     /translation="MKSIKFSLRYPAVTLLLTAAVIIVGIHAFLKMQRTEDPTITIRT
                     GIVAALYPGATSEQVEKQVTKTLEKHIFKFAEVRKEKTYSTSRPGVVIINVDLEDNVK
                     NSDQFWAKLRHEMNLVRATELPAGVMGPVVDSDFGDTVAVLIAVHGKRYGYRELRDYA
                     DKIQDEMRTVRDVGKVVTYGAQAEEIRITSSLERMAQYFADPRQVANALMQRNVIQSA
                     GHFEADGSKIPIRTTGVFNTENEVRSVLVDVSRDGHPVYIKDFADVERRYQDPTFLVR
                     YDGEPCLLLSVEMQKGKNIVELGEQLETVFQRLKVLLPPDVQLDLVANQPGVVKERIT
                     GLSHEFLLAIIAVIIVTIVLLPLRVALIASLAIPISLFGTLGAMESMGLALHQVSIAA
                     LIMVLGIVVDNAIVIVDNYVELLDRKVPLAEAAWRSASDVFVPVLAATITIIASFLPL
                     LILTGSVGEFIMALPITVAIALAVSLIVAVYVTPLLSSYFIKKGLHDHDAAGAQGTAK
                     KGLLDRLQDGYGVLISFFMKKKWLAVSLGVGAVVLGVALFAVVPQQFFPSAERNQFVI
                     DVWMPQGTRIEATDAVMGRIEKALASRKEIVHYATFVGQSAPRFYYNVNPQQPDGAYG
                     QFMINTESVEATSRLVKELRPGLAALAPEAMVIVKELQQGSQMEAPIEVRISGDDITE
                     LKRLGEKVRQILESIPSSQYVYRDYFNDSYMMDVKVNDELANRLGITDASVSQTLSGA
                     FDGGAVSTFWEGDRPVTIKLRL"
     gene            complement(2284..3477)
                     /locus_tag="VFK23_03645"
     CDS             complement(2284..3477)
                     /locus_tag="VFK23_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7318212.1"
                     /translation="MHHNEQHEKIFKYYFQQREGEIMKRIVIIAAGLLAITLPACSRS
                     TTAEQTVSAPMNVKIGKVNPIESHEEVALSGTIAPLAAPSNVSFLVSGKVLHVGPRES
                     DYVRKGEVLASIDPTDYQLALMAAAKQTDMARIAFERAEDEHRRMKMLYDSKSLAPND
                     YEKYKSAYESFGEQFEQATASEAIARKRMNDAVLLAPIDGFISKRAIEPGETAGPGRP
                     VFEIVNLDIVEISVGVPETDVHLVHVGQQAAVTLPALPGESFKGVVRLVNVSADPGTR
                     TFMTRIRIPNPKHTLRIGMVAEARIRGNKVVKMLTAPIEAIVRDPQGATMVYVYYPEQ
                     KRVYARRVEIGAPRDREMEIRSGLGGDEWIVLAGQTKLREGCEVSAVESKERSATTGE
                     EGVRK"
     gene            complement(3609..>4026)
                     /locus_tag="VFK23_03650"
     CDS             complement(3609..>4026)
                     /locus_tag="VFK23_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318213.1"
                     /translation="YVIEIMCYYFWHDRGNNGMQSRAKPGHQVQIFSAIPCVTASDAK
                     EEYCMQQRLRKILFNPVSLIGLITVVSNLGLLVLLSTVDALSGPYANLVIWLIMPWLV
                     FLGLVLIRIGIRPLTEQDAGRQGSRTSLPDRNDRQV"
BASE COUNT          805 a         1190 c         1084 g          947 t
ORIGIN      
        1 cgagccgcag cttgatggtg accgggcggt ccccttccca gaaggtgctc accgcgccgc
       61 cgtcgaaagc gccggacagg gtttgcgata cggacgcgtc cgttattccg agacggtttg
      121 caagctcgtc gttgacttta acgtccatca tgtacgaatc gttgaagtag tcccgataca
      181 catactggga agacgggatg ctctccagga tctgccgcac cttttccccc agtcgcttca
      241 gctccgttat atcgtcaccg gaaatccgta cctcgatcgg ggcctccatc tgggagccct
      301 gctggagctc cttcacgatc accatcgctt ccggcgccaa tgcggccagc ccgggacgca
      361 actcctttac cagccgtgag gtggcctcca ccgactcggt gtttatcata aactggccgt
      421 aggcgccatc gggctgctgc ggattcacgt tgtagtagaa cctcggagca ctctgaccca
      481 cgaaggtggc gtagtgaacg atctctttcc tggaagcaag tgctttttcg atccggccca
      541 tgaccgcgtc ggtggcctcg atccgcgttc cctggggcat ccagacatcg atcacgaact
      601 ggttgcgctc cgcggaaggg aaaaactgct ggggcacgac cgcgaagagc gctaccccga
      661 ggacgaccgc gcccactccc agggagacag ccaaccactt tttcttcatg aaaaagctga
      721 tcaagacacc gtacccatcc tggagccggt cgagcagacc cttctttgcc gtgccctggg
      781 cccccgccgc gtcgtggtcg tgcagcccct tcttgatgaa gtagctcgag agcagcggtg
      841 tcacgtagac ggccacgatg agagaaactg ccagtgctat cgctaccgta atgggaagcg
      901 ccatgatgaa ctcgccgaca ctgcccgtca ggatcaggag cggcaggaac gaggcgatga
      961 tcgtgatggt agcagcgagg accggtacga acacgtcgct tgcgcttctc caggcagcct
     1021 ctgcaagcgg caccttccgg tccagcagtt ccacatagtt gtcgacgatc acgatggcgt
     1081 tgtccacgac gatccccagg accatgatga gcgccgcgat cgagacctga tgtaaggcca
     1141 gccccattga ctccatcgcg ccaagggtcc cgaacaggct tatcgggatg gcaagggatg
     1201 caatgagagc gacccgcaga gggagaagta cgatggtaac gatgatcacg gcaatgatcg
     1261 cgagcaggaa ttcgtggctc agccccgtaa tccgctcttt tacgaccccc ggctggttgg
     1321 cgactagatc cagctgaaca tcagggggaa ggagtacctt gaggcgctgg aataccgttt
     1381 ccagctgttc gcccagttcg acgatgttct tccctttctg catctcaacc gacagaagca
     1441 ggcagggctc gccgtcgtag cgcaccagga acgtcgggtc ctgataccgg cgctccacgt
     1501 cggcgaagtc cttgatatat accgggtgcc cgtctctgga cacgtcgacc agtacgctgc
     1561 gaacttcgtt ttcggtgttg aaaacgccgg tggtcctgat gggaatcttc gatccgtcgg
     1621 cctcgaaatg tcccgcgctc tggatcacgt ttcgctgcat cagggcattc gccacctggc
     1681 gcgggtccgc gaaatactgg gccatccttt cgagactgct ggtgatccgg atctcctctg
     1741 cctgcgctcc gtaggtcacg acttttccca catcgcggac ggtgcgcatc tcatcctgga
     1801 tcttgtccgc gtaatcgcgc agttcgcggt atccgtaccg tttaccgtga acggctatca
     1861 gcacggcgac ggtatcgccg aaatcggaat cgaccaccgg ccccatgacg ccggccggga
     1921 gttcggtggc gcggaccagg ttcatctcat ggcgcagctt cgcccagaac tggtcggaat
     1981 tctttacgtt gtcctccaaa tcgacattta tgatgacgac gccgggacgg ctcgtcgaat
     2041 aagtcttttc cttccgcacc tcggcaaatt tgaagatgtg cttttcgagc gttttcgtta
     2101 cctgtttttc cacctgctcg gaagtcgctc ccgggtacag cgcggccaca attccggttc
     2161 tgatggtgat cgtcggatct tccgtccgct gcattttgag gaaggcatgg atgccgacga
     2221 ttattaccgc tgccgtcagt aagagcgtta ccgccgggta gcggagtgaa aactttatcg
     2281 atttcatttc cgcactcctt cctcgccggt tgtagccgac cgctcttttg attctacagc
     2341 cgatacttca cagccttccc tgagcttcgt ttgtcctgcc agcacgatcc attcgtcacc
     2401 gcccagcccg cttcttatct ccatttcccg atcgcgaggc gcaccgatct cgacacgcct
     2461 ggcgtacaca cgcttctgct ccggatagta cacgtagacc atcgttgcgc cctgaggatc
     2521 gcgcacaatc gcttctatcg gtgcggtgag catcttgacg actttatttc cccggatcct
     2581 tgcttctgca accatgccta ttctgagagt gtgcttgggg ttcgggatgc ggatgcgggt
     2641 cataaacgtg cgggtgccgg gatcggcaga aacattcacg agacggacga cccccttgaa
     2701 tgactccccg ggcaatgccg gcagcgtgac agcagcctgc tgtccgacat gaacgagatg
     2761 cacgtccgtc tcaggcactc cgacactgat ttctacgatg tcaaggttca cgatctcaaa
     2821 caccggccgt cccgggcctg ccgtttcccc cggttcgatc gcccgttttg aaataaatcc
     2881 atctatcggc gcgagaagca ccgcatcgtt catgcgtttc cgggctattg cttctgaagc
     2941 tgtggcctgc tcgaactgtt ctccgaatga ttcatatgcg gacttgtatt tttcataatc
     3001 gttcggggcc agactcttgg agtcgtacag cattttcatc cgcctatgct cgtcctcggc
     3061 tcgttcaaaa gctattcggg ccatgtctgt ttgtttcgcg gccgccatga gtgccagttg
     3121 gtaatccgtc gggtcgatgg aggccagtac ctcacctttc ctgacatagt cgctttctcg
     3181 cggtccgacg tgcagtacct tgccggatac cagaaaagac acattagatg gtgctgccag
     3241 aggagcaatg gtaccgctga gagccacctc ctcatgactc tctattggat tcaccttgcc
     3301 gatcttgaca ttcattggcg cactgaccgt ctgttcggct gttgtgctcc ttgaacatgc
     3361 aggaagggtg atggccaaca agcctgccgc aatgattact attcgtttca taatttctcc
     3421 ctctctttgc tgaaagtagt atttgaaaat tttctcgtgt tgttcgttat gatgcatggc
     3481 gggtccctgg aatttggctg gtcgcccgcg tgcctaaatg agaatagatc cctttcgtgg
     3541 gttggcgttt atcgagatcc attacactaa ttagttgtgt ccccccctct gtctcgaggg
     3601 agagatcgct atacctgacg gtcatttcga tcagggagac tcgttcgcga gccttgcctc
     3661 cccgcatcct gctcggttaa cggcctgatg cctatccgga tcaggaccaa cccaaggaag
     3721 acaagccacg gcatgattag ccagatgaca agattcgcat acggtccgga taacgcgtcg
     3781 accgtggata ggaggacgag gagcccgagg ttggagacga cggtgatcag gccgatgagg
     3841 gaaaccgggt tgaataatat tttcctaagt ctttgttgca tacaatattc ctccttcgcg
     3901 tcggacgccg taacgcatgg aattgcactg aaaatttgaa cttgatgtcc gggcttcgcc
     3961 cgactctgca ttccgttatt gccacgatcg tgccaaaaat aataacacat gatttcaata
     4021 acatac
//
LOCUS       DASSLD010000198         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1040761, whole genome shotgun sequence.
ACCESSION   DASSLD010000198 DASSLD010000000
VERSION     DASSLD010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040761"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFK23_03655"
     CDS             complement(<1..579)
                     /locus_tag="VFK23_03655"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HET7318214.1"
                     /translation="MKVKVLGCSGSETIGHMPPGLLVNDVMMLDAGTITAALGIDAQS
                     RITDILISHTHLDHIKSLLFLADNIIGRTKKPVVIRSIPEVLEAIRIHLMNNIIWPDF
                     TRIPTPNNPVLAYAPLTVGKTVTIAGLKVKAIPMNHPVPAVGFVVGDGTSSLVYSADT
                     GPNERLWKEASKARNLSAIIVDTSFPNSLDGIA"
     gene            complement(765..1577)
                     /gene="trpA"
                     /locus_tag="VFK23_03660"
     CDS             complement(765..1577)
                     /gene="trpA"
                     /locus_tag="VFK23_03660"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET7318215.1"
                     /translation="MSRIKNTFNRLKKKNETALIPYLMAGDPDLATTRTLIVEMEKAG
                     ADIIELGAPFSDPLADGPTIQKAAIRSLRHHTSIADVLSLVADVRKSSPIPLIIMTYY
                     NLIFHYGEERFVSDAAAAGVDGVILPDLPPEEAGALIPPAKKAGLDTIFLLAPTSTEE
                     RIKLVCKVSQGFVYYVSLTGVTGARAGVQSSVEESLRKIKAHTDKPVAVGFGISTPDQ
                     AAQITRWGADGVIVCSALVKVIEEHGSSPELIGKATAFTRALKQGVLAGKVA"
     gene            complement(1630..>2144)
                     /locus_tag="VFK23_03665"
     CDS             complement(1630..>2144)
                     /locus_tag="VFK23_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7318216.1"
                     /translation="DALVACVGGGSNAMGLFYEFIDETSVRMYGVEAGGLGIEPGRHA
                     ARFAGGSLGVLQGSKTFVLQDKDGQIELTHSVSAGLDYAAVGPEHAYYHDTGRIEYAY
                     AVDEEAMEAFDLLSRLEGIIPALESAHAVAYCLKLAPTLGKDKVVIVNLSGRGDKDVM
                     QVAKMKGVEL"
BASE COUNT          381 a          657 c          623 g          483 t
ORIGIN      
        1 ggcgatgccg tcgaggctgt tcgggaatga ggtgtcgacg atgatggccg aaaggttcct
       61 ggcctttgac gcttccttcc agagcctttc gttcggacct gtatcggcgc tgtacacgag
      121 ggacgacgtg ccgtctccca ccacgaaccc gacggcggga accgggtgat tcatggggat
      181 cgccttgacc ttgaggccgg cgatggtcac cgtctttccg accgtcagcg gcgcataggc
      241 cagcaccggg ttgttcggcg tcgggatcct cgtgaaatca ggccagatga tattgttcat
      301 caggtgtatc cggatcgctt cgaggacttc gggaatagac cggatgacca cgggtttttt
      361 ggtccggccg atgatgttgt ccgccaggaa caggagcgat ttgatatggt caaggtgcgt
      421 gtggctgatc agaatgtccg ttatcctgct ttgcgcgtcg atgccgagcg ccgcggtaat
      481 cgtccccgca tcgagcatca tgacgtcatt aaccagcaaa ccaggaggca tatggcctat
      541 cgtctcggac ccggaacaac ccagtacctt caccttcatg cgtcaccctt gatgatgcta
      601 tcattatagt gtgtctgctt catgcggggt cttatcttcg ctgatggact atcccctcta
      661 tcacctctcc ggacatcttc cgcatttcca ggaaccgcgg cggcctgtcc agcggtcttg
      721 attcatcaat gcaccctgcg gttgcacgtg cttcttcggt gagtttatgc aacctttccc
      781 gcaagcacgc cctgtttgag cgcccttgtg aacgccgtag ccttcccgat aagctcgggg
      841 gaactgccat gctcctcgat cactttgacg agcgcactgc atacgataac accgtccgct
      901 ccccaccggg tgatctgggc agcctggtcc ggcgtggata ttccgaatcc cacggcgacc
      961 ggtttgtccg tgtgggcctt gatcttcctg agggactcct cgacggaact ctggactccg
     1021 gcgcgtgcgc cggtaacgcc cgtgagcgaa acataataaa caaacccctg gctgaccttg
     1081 cacacgagct tgatccgctc ctcggtgctc gtcggcgcca ggaggaagat cgtgtcgagc
     1141 cccgctttct tcgccggcgg aatcagcgct ccggcctctt ccggtggaag gtccggcagg
     1201 atcacgccgt ccaccccggc ggcggcggca tcgctcacga accgctcctc cccgtaatgg
     1261 aagatcaggt tgtagtaggt catgatgatg agcgggatcg ggcttgactt ccgcacatcg
     1321 gcgacaaggc tgagcacatc ggcgatcgac gtatggtgcc ggagagaccg gatggccgcc
     1381 ttctggatcg tgggcccgtc cgccagggga tcggaaaagg gcgcgccgag ttcgataatg
     1441 tcggcgcctg ccttttccat ctcgacgatg agcgttctgg tcgtcgcgag gtcaggatcc
     1501 cccgccatga gataggggat aagcgccgtc tcgttctttt ttttgagcct gttgaaggtg
     1561 ttttttattc tcgacatgta cgaggtgtcc tgattttgat tttattccac attccgaatt
     1621 ccgctttcgt tacagctcca cgcccttcat cttcgccacc tgcatcacgt ccttgtcccc
     1681 gcgccccgag aggttcacga tgacgacctt gtccttcccc agtgtcggcg caagcttgag
     1741 acaataggcg accgcatgtg cgctctccag agccgggatg atgccttcga ggcgggacag
     1801 gaggtcgaag gcctccatcg cctcctcgtc gacggcatag gcgtactcga tgcgtccggt
     1861 atcgtggtag taggcgtgct ccgggcccac cgccgcgtag tccagtcccg ctgatacgga
     1921 atgagtgagc tcgatctgac cgtccttgtc ctgaagcacg aacgtttttg atccctggag
     1981 cactccgagg gagcctccgg cgaaacgcgc cgcgtgtctg ccgggctcga tgccgaggcc
     2041 gccggcctcg acgccgtaca tcctgacgga cgtttcgtcg ataaactcgt aaaacagccc
     2101 catcgcattg gagcctccac ccacgcaggc aacgagggca tccg
//
LOCUS       DASSLD010000199         3877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_337439,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000199 DASSLD010000000
VERSION     DASSLD010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337439"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /gene="lpxA"
                     /locus_tag="VFK23_03670"
     CDS             <1..645
                     /gene="lpxA"
                     /locus_tag="VFK23_03670"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HET7318217.1"
                     /translation="LIEGWTTIGERNQIHSFSSIGTPPQDIGYRDEETYLIIGDDNVI
                     RECATVHRATTKEDRKTVIGNRNFLMAYSHVAHDCKLGDYIIMANSVALGGHIEIGDY
                     AIVGGIVAVHQFARIGPYAIIGGQSAVTLDIPPYVSAAGNRAQLYGLNLIGLKRKGFT
                     DQTIATLKKAYKIIFRSGLTQEEAIQKTLDEIHGCPEVNVLVDFIRTSKRGITR"
     gene            733..1554
                     /gene="lpxI"
                     /locus_tag="VFK23_03675"
     CDS             733..1554
                     /gene="lpxI"
                     /locus_tag="VFK23_03675"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163092.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HET7318218.1"
                     /translation="MNKIGLIAGNGQFPIAFARAAKEKGMQVIAVAHEGETLPELAQW
                     VDGIFWIKVGQLGKLITIFHDQGVKDVLMAGGIKKTRLFAGGMPDLRGAAMLAKMIHK
                     KDDSILRSVARELESEGIMVRESTLCLDNIVARRGILTKKKPSKDERRDITFGWEMAK
                     EIGRLDIGQTVVVKDQAVLAVEAIEGTDEAIRRGGLLCGRDAVVVKVCKPQQDLRFDL
                     PAVGALTIQTMERVKASCLAVEADKTIIIERDTVVREAERAGISIVALHDPGDIS"
     gene            1551..2504
                     /locus_tag="VFK23_03680"
     CDS             1551..2504
                     /locus_tag="VFK23_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7318219.1"
                     /translation="MTKLRVGVVGVGYLGRFHAEKYAAFPDVELVGIVDPDLPRIGQI
                     ADTLRTRAFTDPLKLLGQVDAVSIVVPTILHYRIARPFLEQGVHVLLEKPVTVTLEQA
                     DDLIGLAAKKGLILQVGLIERFNPAVTAVRPLIKSPRYLTAERSAPFTVRCTDVSVVL
                     DLMIHDLDIVTDLAGSRPKEVSAAGAAVITGEIDMASARIVFENGCIADVVASRVSDE
                     KKRMLRVFEGGSVYHSDYQSQTASVSRKGTGPVPELSAQTLTGTGKRDTLADELRAFV
                     ESVRTGTMPMVSGAEGRRALALATAIESCIKRGITGFIPVS"
     gene            2528..3634
                     /locus_tag="VFK23_03685"
     CDS             2528..3634
                     /locus_tag="VFK23_03685"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET7318220.1"
                     /translation="MALPVPMVDLKVQYETAKQEITSAVLGVMESTHFILGPQGKALE
                     AEVAAYHDVRHAVSVASGTDALHLALIAAGITRGDEVITTPFTFIATAEAISYVGAVP
                     VFVDIDPVTFNIDAAKVEAAITKKTKAIVPVHLYGQPAEMGRLLSLARERNLQIIEDC
                     AQSFGAEYRGRKTGSFGDFGCFSFFPSKNLGGYGDGGMVITGDQTFAERLLSLRNHGS
                     RVRYYHDEIGYNSRLDEIQAAVLRVKFKRIDEYNERRRKNALSYNKHLSGAGVRTPAE
                     VQETRHVFHQYTIRVKNRDAVKQRLDEGKTTSSMIYYPVPLHLQAAYRDLGMKPGSLP
                     AAEQAAQEVLSLPMYPELTEEQIMTVAEAVKRAL"
     gene            3680..>3877
                     /locus_tag="VFK23_03690"
     CDS             3680..>3877
                     /locus_tag="VFK23_03690"
                     /inference="COORDINATES: protein motif:HMM:NF014714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HET7318221.1"
                     /translation="MKKVLIIAGEASGDLHGANLVREVRKQDPSVLFFGVGSRQMKDA
                     GVQILADASEISVVGATEVLTH"
BASE COUNT          869 a         1124 c         1200 g          684 t
ORIGIN      
        1 ctcatcgagg ggtggacaac gatcggggaa cgaaatcaga tccattcgtt ttcgagcatc
       61 ggaacgccgc cccaggacat cgggtaccgg gatgaagaaa cctacctgat catcggcgat
      121 gacaatgtca tccgcgaatg cgccaccgtt caccgcgcta cgaccaaaga agaccggaag
      181 acggtcatcg gaaacaggaa ttttctcatg gcctactccc acgtggccca cgactgcaag
      241 ctcggcgact atatcatcat ggcgaactct gtcgccctcg gcgggcacat tgagatcgga
      301 gattacgcca tcgtgggcgg catcgtcgcg gtccaccagt tcgcgaggat cggcccctat
      361 gccatcatcg gcggccaatc cgccgtgacg ctggacatcc cgccctacgt gagcgcggcg
      421 ggaaaccgtg cacagctgta tgggttgaac ctgatcgggc tgaagagaaa agggtttacg
      481 gaccagacca tcgcgacgct caagaaggcc tataaaataa tcttccgctc gggcctcacc
      541 caggaagagg cgatccaaaa gacgctcgat gagatccacg gttgcccgga ggtaaatgtc
      601 ctggtcgatt tcatccggac ctcaaaacgg gggatcacga ggtaggaaca caatgcggaa
      661 ttcggaatgc ggaatgcgga atgaaaggtc aatcggcaaa accgccgggt tcgtaacccg
      721 gctgtgcaaa gtatgaacaa aatcggttta atagcaggaa acggacagtt ccccatcgcc
      781 ttcgcccggg ccgcaaaaga aaaaggaatg caggtcatcg ccgttgccca cgaaggcgaa
      841 accctccccg agcttgcaca gtgggtagac ggcatcttct ggatcaaggt cggccagctc
      901 ggaaagctca tcacgatatt ccacgatcag ggagtgaagg acgtgctcat ggccggcggg
      961 atcaagaaga cgcgcctctt tgcgggaggt atgcccgacc tgaggggcgc ggccatgctc
     1021 gccaagatga tccacaagaa ggacgattcg atcctccgct ccgtcgcgcg agaactcgaa
     1081 tccgagggca taatggtgcg tgaatcaacg ctttgcctgg acaacatcgt ggcccggcgg
     1141 ggaattttga cgaagaaaaa accgtcgaag gatgaacggc gggacatcac gttcggctgg
     1201 gaaatggcaa aggagatcgg caggctcgac atcggtcaga cggtggtggt gaaagaccag
     1261 gccgttctcg ctgtcgaggc gatcgagggc accgacgagg cgatccgccg gggcggatta
     1321 ctctgcggac gggacgccgt cgtggtcaag gtctgcaagc cccagcagga ccttcgtttc
     1381 gacctccccg ccgtcggggc cctgacgatc caaacaatgg agcgggtcaa ggcctcctgt
     1441 ctcgcggttg aagcggacaa gaccattatc atcgaacgcg acacggtggt ccgggaagcc
     1501 gagcgggcgg gcatctctat cgtggcactt catgaccccg gggacatttc atgacgaaat
     1561 tgagggtggg cgtagtcggc gtcggatacc tcggccgctt tcacgccgaa aaatatgcgg
     1621 catttcccga cgtcgaactc gtcggcatcg tggaccccga cctgccccgg atcgggcaga
     1681 tcgcggatac cttgcggacg cgggccttca cggaccccct gaagctcctc gggcaagtgg
     1741 acgcggtgag catcgtcgtg ccgacgatcc tgcactaccg catcgcgcgg cctttcctgg
     1801 aacagggcgt ccatgtcctt ctcgaaaagc ccgtcaccgt gaccctagag caggcggatg
     1861 atctcatagg gctcgctgcg aaaaaaggac tgatactcca ggtggggctc atcgaacggt
     1921 tcaacccggc tgtgaccgcc gtcagaccgc tgatcaagtc cccccggtac ctgaccgcag
     1981 aacggtcggc gccgttcact gtccgctgca ccgacgtcag cgtcgtgctg gacctcatga
     2041 ttcacgacct cgatatcgtc accgacctcg cgggctcgag gccgaaagag gtgagcgcag
     2101 ccggcgcagc ggtcatcacc ggcgagatag acatggcatc ggcgcggatt gtttttgaaa
     2161 acggctgtat tgcagatgtc gtcgcgagcc gcgtctcgga cgagaaaaaa cggatgctgc
     2221 gggtgtttga aggcgggagc gtgtaccact cggattacca gtcccagacc gcgtcggttt
     2281 cgcgcaaagg gacgggtccg gtccccgaac tctcggccca gaccctgacc ggaacgggaa
     2341 agcgcgatac cctcgccgac gagctcaggg ctttcgtgga gagcgtcagg accgggacca
     2401 tgcccatggt atcgggcgct gagggaaggc gtgccctggc cctggcaacg gccatagaat
     2461 cctgtatcaa acgggggatc accgggttta ttccggtgtc ctagtcaaga aaggagtttt
     2521 tttcatcatg gccttacccg tacccatggt ggaccttaag gtccagtatg agaccgcaaa
     2581 gcaggaaata accagcgccg tgctcggcgt catggagtcc acccatttca tcctcggacc
     2641 ccagggaaag gcgctcgagg cggaggtcgc ggcttaccat gacgtcaggc acgctgtgtc
     2701 ggtcgcctcg ggcacggacg cgctgcacct ggcgctcatt gcggcgggta tcacgcgcgg
     2761 agacgaggtc attacgacgc cgtttacgtt catcgctacg gcagaggcga tttcctatgt
     2821 cggcgccgtg ccggtgttcg tggacatcga cccggtaacc ttcaatatcg acgcggcgaa
     2881 ggtcgaggct gcgatcacaa agaaaacaaa ggccattgtc ccggtccacc tctacggcca
     2941 gcccgcggag atggggcggc ttctgtcgct ggcgagggaa aggaaccttc agatcatcga
     3001 agactgcgca caatcgttcg gcgcggagta ccggggccgg aagaccggtt ccttcggcga
     3061 tttcggctgt ttcagcttct tccccagcaa gaacctgggc ggatacggcg acggcggcat
     3121 ggtgatcacc ggcgatcaga ctttcgcgga gcgcctgctg agcctgcgga accatgggag
     3181 ccgggtccgg tactaccatg atgaaatcgg atataacagc aggctcgacg agatccaggc
     3241 ggcggtcctc cgggtcaaat tcaagcgcat tgatgaatac aacgagagac gtcggaagaa
     3301 cgccctttcc tacaacaaac atctctccgg ggccggcgtc cggacccccg ccgaagtgca
     3361 ggagaccaga catgttttcc accagtacac gatccgggtc aagaacaggg acgctgtcaa
     3421 gcagcggctc gatgagggca agacaacatc ttcgatgatc tactatcccg tacccctcca
     3481 tctccaggcg gcgtaccggg atctcggcat gaagccaggc agcctgcccg ccgcggaaca
     3541 ggcagctcag gaagtcctgt cgcttccgat gtatcccgag ttgacggagg agcagatcat
     3601 gacagtggct gaggcggtga agagggcgct gtaaatcagg agtctgaagc cagaaaccag
     3661 gagtcagaag ttcgaacata tgaagaaagt cctcatcatc gccggcgagg catcgggcga
     3721 tctgcatggc gccaatctgg tgcgggaggt caggaaacag gacccttcgg ttctcttctt
     3781 cggggtcgga agcaggcaga tgaaggacgc gggcgttcaa attctggccg atgcatccga
     3841 gatatccgtc gtcggcgcca cggaagtgct gactcat
//
LOCUS       DASSLD010000200         5055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_757378,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000200 DASSLD010000000
VERSION     DASSLD010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757378"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..488)
                     /locus_tag="VFK23_03695"
     CDS             complement(54..488)
                     /locus_tag="VFK23_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HET7318222.1"
                     /translation="MLTTQTKQLRKKDKAVRFKRTPQRLAILEYLDGNTSHPSAEDIY
                     RAVGKKYASMSFATVYNTLHALTQAGALRELTIDPERKRYDPDTTRHHHLICLACGKI
                     FDVPEEIRVDLPKSMQEDFTVVANHVEFYGHCSPCGKKRKAP"
     gene            complement(623..1048)
                     /locus_tag="VFK23_03700"
     CDS             complement(623..1048)
                     /locus_tag="VFK23_03700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET7318223.1"
                     /translation="MVAEKGKMLLEIVAPSRQVVRSERVDEVIAPGSEGEFGVLPGHT
                     PFLTTLKVGMLSYREGSAWHHLAVDWGYAEVGPDRVVILAEGADRASEIDLSEARLAK
                     ERAERALAEKLEKTDYEKARIDLLRAMIRIQVAEKHGQK"
     gene            complement(1160..2581)
                     /gene="atpD"
                     /locus_tag="VFK23_03705"
     CDS             complement(1160..2581)
                     /gene="atpD"
                     /locus_tag="VFK23_03705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET7318224.1"
                     /translation="MSTGKIVQVMGAVVDCEFPVGSLPQLMNALRIDQAEDKSLGTPE
                     THLTLEVALHLGENRVRTVAMSSTDGLMRGMKVNDTGKPISMPVGKEVLGRIMNVIGE
                     PVDEMGPIQTKDTLPIHRSAPMLEELDPTKQLFETGIKVVDLLAPYTKGGKTGLFGGA
                     GVGKTVIIMELINNVAKQHGGFSVFAGVGERTREGNDLWAEMKESGVISKAALVYGQM
                     NEPPGARLRVGLSALTVAEYFRDTMGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVG
                     YQPTLGTEMGALQERITSTKKGSITSVQAIYVPADDLTDPAPATAFAHLDATTVLSRQ
                     IAELGIYPAVDPLDSTSRIMDPHVVGEEHYSVAQRVQLVLQRYKELQDIIAILGMDEL
                     SDEDKLTVSRARKIQRFLSQPFQVAEAFTGTPGKYVPLKETIKGFKELVDGKMDDIPE
                     QAFYMVGPIEEVFEKAEKLGYKR"
     gene            complement(2690..3556)
                     /gene="atpG"
                     /locus_tag="VFK23_03710"
     CDS             complement(2690..3556)
                     /gene="atpG"
                     /locus_tag="VFK23_03710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HET7318225.1"
                     /translation="MPSLRDIKRKITSVKKTQQITKAMKMVAAAKLRRAQERVIAARP
                     YSRKMLAVIANLAGRVERAQHPLLAKRAPKQVKLVVLTSDRGLCGAYNTNILRKAVEA
                     VRQFKADGKEVKVNVVGRKGRDFFRKRPGYTLGKVWSDLGMIDYDKAADVGKNIVERF
                     TAGETDEVYLLYNEFKSVMQQKVTLEKLLPVEPPAEADPFMAAVDYLYEPTPEAILSS
                     ILPKHIEVQVYRSMLESQASEMGARMTAMDSATRNAKDMIERLTLKFNKQRQAAITKE
                     ISEIVGGAEALK"
     gene            complement(3666..>5055)
                     /gene="atpA"
                     /locus_tag="VFK23_03715"
     CDS             complement(3666..>5055)
                     /gene="atpA"
                     /locus_tag="VFK23_03715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET7318226.1"
                     /translation="VFGLDNVMSGELLELPGGLMGMALNLEEENVGVVLMGDDSKIRE
                     GDIVKRTKRIAEVPVGDAIVGRVVDGIGQPIDGKGPIVSKEFRKIEVIAPGIVKRQSV
                     RESLQTGIKALDAMIPIGRGQRELIIGDRQTGKTAVAIDTIINQKGQNVTSIYVAIGQ
                     KRSTVAQVVKTLSEHGAMDYTIIVSATASDPAPLQFIAPYTGCTIGEYFRDSGKHALI
                     VYDDLSKQATAYRQLSLLLRRPPGREAYPGDVFYLHSRLLERAAKLSDKMGGGSLTAL
                     PIIETQAGDVSAYIPTNVISITDGQMFLESDLFYSGVRPAINVGISVSRVGGSAQIKA
                     MKQVAGTLRLDLAQYRELAAFAQFGSDLDKATLAQLNRGQRMVELLKQDQYVPMLVEH
                     QILVIFAGTNGYVDDVPVNAVKKFEAELLKFVTSKHQALLSDIKTKKQIDDDLKGRAK
                     AAIEEFKKTFTA"
BASE COUNT          881 a         1521 c         1493 g         1160 t
ORIGIN      
        1 atttcacgat ttgattgttc agggtttcgc acttgggatt tagtgcctga tctttacgga
       61 gccttgcgct tctttccgca gggcgagcag tgaccgtaga attcgacatg gtttgcgacc
      121 acggtaaaat cctcctgcat ggacttgggc aggtccacgc ggatctcttc ggggacatcg
      181 aatattttcc cgcaggcgag gcagatcaga tgatggtgcc gggtcgtgtc ggggtcgtac
      241 cgctttcttt ccgggtcgat ggtgagttct cggagcgccc ctgcctgcgt cagcgcatgg
      301 agcgtgttat acaccgtcgc gaacgacatg gacgcatact tctttcccac ggcccggtag
      361 atgtcctcgg ccgagggatg ggaggtgttc ccgtcgagat actccaggat cgcaagcctc
      421 tgcggggtcc gcttgaagcg cacggccttg tctttcttcc gtaactgttt tgtctgcgtc
      481 gtgagcatgt tgtttggaat gattataaag taatgcgggg catgtgtcaa gctttttcgg
      541 ccgggataaa aaaaggcaga ccccaagggg ctgcctttcg atgaacttgt ctcatcagag
      601 aacttgtcct ctctgacggg gtctacttct gtccgtgctt ctcggccacc tgaattctga
      661 tcatcgccct caagaggtcg atgcgggcct tttcgtaatc cgtcttttcc agcttctcgg
      721 cgagcgccct ttccgcgcgc tccttggcga gccgggcttc gctgaggtca atctccgatg
      781 cccggtccgc gccctccgca aggatgacga cccggtcggg gcccacctct gcataccccc
      841 aatcgaccgc gaggtgatgc catgcggagc cctcccggta ggaaagcatg ccgaccttga
      901 gcgtagtcag aaacggcgtg tggcccggca gcacgccgaa ttcgccttca ctgcccgggg
      961 cgatcacctc gtccacacgc tcggagcgga ccacctgccg gctcggcgca acaatctcga
     1021 ggagcatttt ccctttttca gcgaccatga tcttcttccg ttcctcgctc attaaaatca
     1081 ctgatgcagg atgcatgatc catgatgcat gattgagctt tctgttctat cctgcatcct
     1141 gtatcctgta tcttcagcgt taccgcttgt acccgagttt ctcggccttc tcgaatactt
     1201 cctcgatggg gccgaccatg tagaaggcct gctcggggat gtcgtccatc ttgccgtcaa
     1261 caagttcctt gaaacccttg atggtttcct tgagcggcac atatttaccc ggagtgccag
     1321 taaaggcctc ggccacctgg aagggctggg acaggaagcg ctggatcttc ctcgcccgcg
     1381 atacggtgag cttgtcttcg tctgagagct cgtccatacc caggatcgcg atgatgtcct
     1441 gcagttcctt gtaccgttgg agtacgagct gcaccctttg cgcaacagag taatgctctt
     1501 cgccgaccac atggggatcc atgatccggg acgtcgaatc gagggggtcc acggcggggt
     1561 agatgccgag ctccgcgatc tggcgggaaa gcacggtggt ggcgtccagg tgcgcgaagg
     1621 ccgttgccgg cgccgggtcg gtcaggtcgt ccgcgggaac atagatggcc tgcaccgagg
     1681 tgatggagcc cttcttcgtt gaggtgatgc gctcctgcag cgcgcccatt tcggtgccga
     1741 gggttggctg ataacccacg gccgacggca tacgaccgag gagcgccgac acctcggaac
     1801 ccgcctgggt gaaccggaat atattgtcga cgaacaggag cacgtcctgc cccatcgtat
     1861 cgcggaaata ctcggcaacc gtgagggcgg agaggccgac gcgaagtctc gctcccggcg
     1921 gctcgttcat ctggccgtag accagggccg ccttgctgat aacgcccgac tccttcatct
     1981 cggcccagag gtcattacct tcacgggtac gctcgcccac acctgcgaac acggagaacc
     2041 cgccgtgctg ctttgccacg ttattgatga gttccatgat gatcacggtc ttgcccacgc
     2101 ccgcgccgcc gaagaggccc gtcttgccgc ccttggtgta gggtgcgagg aggtcgacga
     2161 ccttgatgcc ggtctcgaag agctgcttcg tgggatcgag ttcctcgagc atcggggcgg
     2221 accggtggat gggcagcgtg tccttggtct ggatcggccc catctcatcc acaggttcac
     2281 cgatcacgtt cataatgcgg cccagcacct ccttgcccac aggcatggaa atgggcttgc
     2341 cggtgtcgtt gactttcata ccgcgcatga gaccgtcggt ggacgacatg gccacggtgc
     2401 gcacccggtt ctctccgaga tggagcgcaa cctcgagcgt cagatgggtc tcgggcgtac
     2461 cgaggctctt atcctcggcc tgatcgatcc tgagcgcgtt catcagttgg ggaaggcttc
     2521 cgacggggaa ctcacagtcc accaccgcgc ccatcacctg tactattttc cctgtgctca
     2581 ttgtaacccc ctgtttgata ttgctatgac tcggcgacgc ggcgaatggg cgagggggcg
     2641 aaaaagcttt ttctcccact cttcgtttcg ccccttctcc gcgtcgggtt tatttcaacg
     2701 cttccgcgcc gccgacgatc tcggagattt ccttggtgat cgcggcctgg cgctgcttgt
     2761 tgaacttcag cgtgagccgc tcgatcatgt ccttggcgtt ccgcgtggcg gagtccatcg
     2821 ccgtcatgcg cgctcccatc tcgctggcct ggctttcaag catggaacgg tatacctgaa
     2881 cctcaatgtg cttcggcaag atcgaactca ggatcgcctc cggtgtcggc tcatagaggt
     2941 aatcgacagc agccatgaac ggatcagctt cagccggcgg ttctaccggc aggagcttct
     3001 cgagcgtcac cttctgctgc atcacgctct tgaactcgtt gtagagcaga tacacctcat
     3061 cggtctcgcc cgccgtaaaa cgttcgacga tgttcttgcc gacatcagcc gccttgtcat
     3121 aatcgatcat gccgagatcc gaccacacct tgcctagcgt atagccggga cgtttgcgga
     3181 aaaagtcgcg tccttttcta cccacgacat tcactttcac ttctttgcca tcggccttga
     3241 actgtctgac ggcctcgacg gctttgcgca ggatattggt attgtacgcg ccgcagagcc
     3301 cccgatccga ggtgagcacg acaagcttca cctgcttggg cgcccgtttc gcaagaagcg
     3361 ggtgctgggc cctctccacg cggcctgcga ggttcgcgat caccgcgagc atcttgcgcg
     3421 aataaggccg cgccgcgatc acgcgttcct gggcgcgccg cagcttcgcc gcagccacca
     3481 tcttcatggc cttggtgatc tgctgggttt tcttgacgct cgtgatcttt ctcttaatat
     3541 ctcttaagct gggcatgaaa agtcctttag cttctttctc accgcggagg tcgcggagta
     3601 cgccgaggta aagcctgttt catttatttc tctgcgatct ctgcgttctc tgcggtaaaa
     3661 gtcttttacg ccgtgaacgt cttcttgaac tcctcgatag ccgccttcgc gcgtcccttg
     3721 agatcgtcgt cgatctgctt tttggttttg atatcgctca agagcgcctg gtgtttgctc
     3781 gtcacgaact tcaggagctc ggcctcgaac ttcttcaccg cattcaccgg gacgtcgtcc
     3841 acatagccat tggtccctgc gaagatcacg aggatttggt gctcaaccaa cataggcacg
     3901 tactggtcct gcttaagaag ttcaaccatg cgctgaccgc ggttcaattg ggccagtgtg
     3961 gccttgtcca ggtcgctgcc gaactgggcg aacgccgcga gctcgcggta ctgcgcgagg
     4021 tcgagccgaa gggtaccggc cacctgcttc atggccttga tctgggcgct gccgcccacg
     4081 cgcgacaccg agatacccac gttgatggcc ggacgcacgc cggagtagaa gaggtcggac
     4141 tccaggaaca tctgtccgtc ggtgatggag atcacgttcg tcgggatgta ggcggacacg
     4201 tcgccggcct gggtctcgat gatcggaagt gccgtgagcg atccgccgcc catcttgtcg
     4261 gacagctttg ccgcgcgctc gaggagacgc gagtggagat agaacacgtc gccgggatag
     4321 gcttcgcggc ccggagggcg gcgaaggagc agtgagagct ggcggtaggc cgtggcttgc
     4381 ttcgacagat catcatagac gataagggcg tgcttgccgc tgtcgcggaa atactcgccg
     4441 atagtgcagc cggtgtacgg ggcgatgaac tggagagggg cggggtcgga agcggtggcc
     4501 gacacgatga tggtatagtc catagccccg tgctcgctga gggtcttgac gacctgcgct
     4561 acggtggagc gcttctggcc gatggccacg tagatgctgg tgacgttctg gcccttctgg
     4621 ttgatgatgg tgtcgatggc aacggcagtc tttccggtct ggcggtcgcc gatgatcagc
     4681 tcgcgctggc cccggccgat ggggatcatg gcatcaaggg ccttgatgcc ggtctggagc
     4741 gactcgcgca ccgattgccg ctttacgata cccggcgcga taacctcgat cttgcggaat
     4801 tccttcgaga cgatgggacc cttgccgtcg atgggctggc cgattccatc taccacgcgg
     4861 ccgacgattg cgtcgcccac gggcacctcg gcgatcctct tggtccgctt cacgatgtcg
     4921 ccttcgcgga tcttcgagtc gtcgcccatg agcacaacac cgacgttctc ttcctcgagg
     4981 ttgagcgcca tgcccatcag gccgccgggg agttccaaaa gctcgccgga catgacgttg
     5041 tcgagtccga agacc
//
LOCUS       DASSLD010000201         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_798077,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000201 DASSLD010000000
VERSION     DASSLD010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798077"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VFK23_03720"
     CDS             <1..918
                     /locus_tag="VFK23_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET7318227.1"
                     /translation="YSLVAASGAVALLLFGRIRTETILEGVDWTLLLFFSGLFVVMHG
                     VEASGLAAAVIAKAGDLTSLSSFWQIAGLSTVSVVLSNLVSNVPAVMLLKSLTQSLGN
                     GHVAWLALAMSSTLAGNLTLIGSVANLIVAQQAARRVEIGFIEYFRVGALITLVTVAV
                     GVLVLFVEVRMAHGAEANAAKSGLSLKTVAITPVDSASATREYRIVLLCDTDASRERG
                     LQGFRPLKRDEAALFVFQKPVAVTFWMGSVAYPIDIIFVGPDRKVVRVYPNCRPRSPE
                     YYPSTAPVSWVIETAAGSGIRSGDRVSFK"
     gene            1047..1892
                     /locus_tag="VFK23_03725"
     CDS             1047..1892
                     /locus_tag="VFK23_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7318228.1"
                     /translation="MRMVAAAGLLSAAIPAGPACAGERSTAVVIGGTGSALGGMKEIA
                     KAFRKEHPEITVSFVPSLGSSGGIEAALSGSLDLALSARPLTDSEQERGASAVEYART
                     PFLFTTKDASHAASLTLRKVASIYGGEAPQWPDGTPLRLVLRPEKDMDTLLLRSMSPE
                     MDIAVQKAVRRQGMLVAVTDQENADALVKGKGAFGATTLAQILAEKRPLIPLTLDGVA
                     PGVDALAEGRYPFSKVFYAISTPRSSSATRQFIEYLVSPKARAILARTGHLVSARPGT
                     AGRRE"
     gene            1906..>2674
                     /locus_tag="VFK23_03730"
     CDS             1906..>2674
                     /locus_tag="VFK23_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7318229.1"
                     /translation="MKRITAAAIRTTTRLALGLSLVLALLAPLGYLTMAYERQRSVIE
                     TEAEAAASALAQLISANPEFWRYEMPRLESFLSRRPLDGHREIRRIVGLDNAVLAERT
                     DALDRPFVTRSSPLYDSGKVVARIEISRSLRPILIRTLGLALLGLGFGAGAFILLWTF
                     PLRALQGTLRSLQESEAKFRAIAETAADGVIVMDGGGRVAYWNHAAEQMFRHSVHEVM
                     GEDLHRLISPPSYHEQFRRGFALFTRTGQGPAIGNTLE"
BASE COUNT          489 a          800 c          822 g          563 t
ORIGIN      
        1 tattcgctcg ttgccgcgtc cggtgccgtg gcgcttctgc tcttcgggag gatcaggacg
       61 gagacgattc ttgaaggcgt cgactggacg cttctgcttt ttttctcggg tctgttcgtg
      121 gtgatgcacg gagtggaggc atcgggcctg gcagcggcag tgatcgcaaa ggccggtgat
      181 cttacgtctc tctcctcctt ctggcagata gccggattgt ccacggtttc ggtcgttctc
      241 tcgaacctgg tcagtaacgt tcccgccgtg atgctcctga aatcgcttac gcagtcgctg
      301 ggcaacggtc atgtcgcgtg gctggcgctg gccatgagca gcacccttgc cggcaacctg
      361 accttaatcg ggtcggtcgc gaacctcatc gtcgcgcaac aggcggcaag acgcgtcgag
      421 atcgggttca tagaatactt tcgcgtcggc gcgctcatca cgctcgtgac ggttgccgtc
      481 ggggtcctgg tcctgttcgt cgaagtacgc atggcccatg gggcggaagc gaacgcggcc
      541 aaatccggtt tgtcgcttaa aacggtcgcc ataacacccg ttgacagcgc gtcggccacg
      601 cgggagtacc gaatcgtgct cctctgcgat acggacgctt cccgcgaacg gggcctgcag
      661 ggctttcgtc cgctcaagcg cgatgaagcc gccctgttcg tatttcaaaa accggtagca
      721 gtcaccttct ggatgggaag cgtcgcctat cccattgata tcatctttgt cggccctgac
      781 agaaaggtgg tccgggttta tccgaactgc cgtcccagga gcccagaata ctatccttcg
      841 accgcgccag taagctgggt gatcgagacc gcggccggtt caggaatcag gtccggggac
      901 agggtgagct tcaagtagga tctttttgat tacgaaaaat caagacagat tattgcactt
      961 tatcgttgtt catgtgataa taacccatgc ttgctcaaaa acagtccgtt ggtcacgtct
     1021 cccttcgggt caggatgatg ttttggatgc gcatggtcgc ggccgcaggt ctgctgtccg
     1081 cggccattcc ggccggccct gcgtgcgccg gcgagagaag tacggccgtc gtgatcgggg
     1141 gcaccggtag cgccctgggt ggcatgaagg agattgccaa ggccttccga aaagagcatc
     1201 cggaaatcac ggtctcgttc gtgccgagcc tcggcagcag cggggggatc gaggcggctc
     1261 tttcggggtc ccttgatctt gcgctctccg cccgtcctct cacagacagt gagcaggaac
     1321 gaggcgcatc ggcagtcgag tacgcccgca cgccttttct cttcacgacg aaggatgctt
     1381 cgcatgcggc ttccctgacg ctccggaagg tcgcctcgat ctacgggggg gaggcgccgc
     1441 agtggccgga cggaactccg ctgcgtctgg ttcttcggcc ggaaaaagat atggacaccc
     1501 ttctcctgag gtcaatgtct cccgaaatgg acattgccgt tcaaaaggcg gtgagacgac
     1561 agggaatgct cgtcgccgtc acggatcagg agaacgccga tgccctcgtc aagggcaagg
     1621 gggccttcgg cgccacgacg ctggcccaga tcctcgccga gaagaggccg cttatcccgc
     1681 tgacgctgga cggtgtggcg ccgggcgttg atgcgcttgc agagggtcgc taccctttct
     1741 ccaaagtctt ttacgcaatc tccacgcccc gcagctcttc ggcgacgcgt caatttatcg
     1801 aatatctggt ttccccgaag gccagggcca ttcttgcgag gacgggccat ttggtctccg
     1861 cgaggccggg gaccgcaggc cgccgcgaat gaacaggggt aaaccatgaa gcgaataaca
     1921 gcggcagcca tcaggacgac aacgcgcctt gccttagggc tttccctcgt tcttgcgctc
     1981 ctcgctcccc tcggctatct gacaatggct tacgagcggc agagatcggt gatcgagacc
     2041 gaagctgagg cggccgcatc tgcccttgcc cagctcatca gcgccaatcc cgaattctgg
     2101 cgctacgaaa tgccgcgatt ggaaagcttt ctgtcgcgcc gtcccctgga cgggcatcgg
     2161 gaaatccggc gcatcgtggg cctcgacaac gccgtccttg cggagcgcac agatgccctt
     2221 gatcgcccct ttgtcacacg ctccagtccc ctgtacgatt cgggaaaggt cgtagcgaga
     2281 atagagatca gccgctccct ccggcctatt ctgataagaa ccctgggtct ggcgctgctc
     2341 gggctcggtt tcggcgccgg cgcctttatc ctcctctgga cgtttcccct gcgtgcactc
     2401 caggggacgt tgagatcgct gcaggaaagc gaggccaagt ttcgagccat cgccgaaaca
     2461 gccgcggatg gagtgatcgt gatggacggc gggggacgcg ttgcgtattg gaatcacgca
     2521 gcggaacaga tgttcaggca ttctgtgcat gaggtcatgg gcgaagacct tcaccggctc
     2581 atcagcccgc cgtcctatca tgagcagttc cggcggggtt tcgccctttt cacgaggaca
     2641 ggacaaggac cggcgatcgg gaacaccctc gagt
//
LOCUS       DASSLD010000202         2534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_864236,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000202 DASSLD010000000
VERSION     DASSLD010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864236"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="VFK23_03735"
     CDS             complement(<1..177)
                     /locus_tag="VFK23_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HET7318230.1"
                     /translation="MLFVLFATALFSLLGCPPQESKPVKTVTIPDGTIEPAEWGKAYP
                     TEYELWKKTEVPEPA"
     gene            complement(253..717)
                     /gene="nrfH"
                     /locus_tag="VFK23_03740"
     CDS             complement(253..717)
                     /gene="nrfH"
                     /locus_tag="VFK23_03740"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HET7318231.1"
                     /translation="MDRARTWKVIKVIALACIAGAAVMVFLMFGPPKLLAKSESPDFC
                     SKCHVMGPEYQAWSHMGAHRRKLCVDCHLPNGNMGIHYIWKALDGLKDVLFFYSGSVP
                     ERIRLTAHGEKVLQANCIRCHESSVEFINHERKCWECHRRLIHTRSGSMETL"
     gene            complement(1158..2276)
                     /locus_tag="VFK23_03745"
     CDS             complement(1158..2276)
                     /locus_tag="VFK23_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HET7318232.1"
                     /translation="MCDTREVKSPDITATKTIPVGEAVGMVLAHDVTEIRKDEFKGRA
                     FKKGHIIRAEDIDHFRKLGKEHLFVLSLGSDEIHEDDAALALASAIMGAGVRIEGEPR
                     EGKINIIAERDGLLKVNRDSLLEFNMLGDVMCATRHGNTVVKKGRVVAGTRAIPLVVK
                     RGIIEEAISIGERARSSGTPLVEVKELRKPKTGIVITGNEVYYGKIRDAFAPVITKKI
                     EELGGDIVGIYYAPDDAVFIEQRLRELVNSGADLLIATGGMSVDPDDVTRFAIRNLGA
                     ADMTYGSAVLPGAMVLVAYLSKEHGAAGMEKESSAMIPILGIPACGMYHKITVFDLLL
                     PRVLAGDKIGRRELAELGHGGLCLNCKTCRYPVCPFGK"
BASE COUNT          569 a          704 c          603 g          658 t
ORIGIN      
        1 cgccggctca ggcacttccg tctttttcca gagctcatat tccgtcggat aggccttccc
       61 ccattcggcg ggctcgatcg ttccgtccgg aatggtcacg gtcttgacgg gtttgctttc
      121 ctgcggggga caccccaaca gggaaaataa agcggttgcg aacagcacga acagcattga
      181 tacaacagat gatctcttca tggctgaacc tccttcaggg gatcaggatg atattcgctg
      241 agattgatat ggtcatagtg tttccattga gccgctccgg gtatgaatga gcctgcggtg
      301 acattcccag cactttcttt catgattgat gaactcgacc gagctttcat ggcagcggat
      361 gcagttggcc tgcagtacct tctcgccgtg agcagtcaat ctgatccgtt ccggaacact
      421 gccggagtaa aaaaatagca catctttcaa tccgtccagt gccttccaga tatagtggat
      481 gcccatattt ccgtttggca agtggcagtc cacgcacagt tttctccggt gtgctcccat
      541 gtgcgaccac gcttgatact ctggccccat cacgtggcat ttggagcaaa aatcagggga
      601 ttctgatttg gcaagcagtt tgggcggacc gaacatgagg aaaaccatca ctgcggcccc
      661 ggcgatgcat gcaagggcaa taactttaat aactttccag gttctggccc tatccatctt
      721 attcgcctcc ttttaataaa aatacctcac tatcaattgg ggcgcaaatt gtagcaataa
      781 attgaataaa aaatttgttc tttaccgatg acatatgaga gattcgacat gctgatttta
      841 ttttatcata aaaaagataa gatgttcctt aatctcgtga gagtttttgc ctatttaggg
      901 gaaacacgta gcatgagaca tgatatgcac tattgtggtg atctttccaa ccggttccgc
      961 attctagaat atgccagtat gccctgtgtt ttgacacggg gataaatggc aaccttgcca
     1021 aatggatcgc tataaaaaaa tggcctccca ctttatgggg aggccatttt cccgatgttt
     1081 tcatacagaa agaaaaccct acaaggcatc aaggcgtttt tttattctcc tcttcggaga
     1141 tgttcaatga gtctttgtta ttttccgaac gggcagaccg gatacctgca cgttttacag
     1201 ttgagacaga gtcctccatg ccccagttca gcgagctccc ttctgccgat cttgtctccg
     1261 gcaagcaccc tcggtagaag aaggtcaaaa accgtgatct tatgatacat gccgcaagcg
     1321 ggaatgccga ggatggggat catggcagag gattcttttt ccatacccgc tgcgccatgc
     1381 tctttgctga gataggctac cagcaccatc gcacccggca agacggccga gccgtacgtc
     1441 atatcagcag cgccaaggtt acgtatcgcg aaccttgtta catcatcggg atccacggac
     1501 attccgccgg tggcaatgag gaggtccgca ccagaattaa ctagctcacg cagtctctgc
     1561 tcgatgaaga cagcatcatc aggcgcatag tagatgccga cgatatcgcc gcctaactct
     1621 tcgatctttt tagttatcac gggcgcgaag gcgtctcgta tcttcccgta atagacttca
     1681 ttgccggtga tcacgatgcc cgttttcggt tttctcagct ctttgacctc cacaagtgga
     1741 gtgccggaac tccgggcccg ctcaccgatc gatatcgcct cctcgattat gccccgctta
     1801 acgacgagcg ggatcgccct cgtaccggcc acgactcgac ccttcttcac aacagtgttg
     1861 ccgtgacgcg tggcgcacat cacatcgccg agcatgttga attcgagaag ggagtcccgg
     1921 ttcaccttga ggagcccgtc ccgttcggct ataatattga tcttcccttc ccgtggctcg
     1981 ccctcgatcc taactcccgc gcccataatt gccgaggcga gcgctaacgc tgcatcgtcc
     2041 tcatgtattt catcggagcc gagcgagagg acgaagagat gctccttccc gagcttccgg
     2101 aagtggtcga tgtcttcagc gcgtatgatg tggcctttct tgaatgccct gcctttgaat
     2161 tcgtccttcc tgatctccgt cacgtcgtga gccaggacca tgccgacagc ctctccaacg
     2221 ggtatagtct tcgtcgcggt gatgtccggg cttttaacct cgcgggtatc gcacatgtct
     2281 tctctccttg ccgtgtacag atatgagttg aaccccactc ccgaatatct cttcaaattt
     2341 tttgtccacc acttccctta aaccatcttc catgtgctca aacctatgga ggaattcccg
     2401 ggcgtcactg gtgaggaccg cgcagcgcgg ttgatcgaac catgggcgtc tatcgcttcg
     2461 agcaaaaagc gtcggcccct gccgaacacc ggctcgcctt tcgcctctat ccagaccttg
     2521 gatctgatct ccaa
//
LOCUS       DASSLD010000203         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_483473,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000203 DASSLD010000000
VERSION     DASSLD010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483473"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1989
                     /locus_tag="VFK23_03750"
     CDS             262..1989
                     /locus_tag="VFK23_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HET7318233.1"
                     /translation="MSRRLQEKTDALIAAERGAVIKPQGADIAVALAYPNTYHVGMSN
                     LGLHQIYALLNSRPDTACERVFLPDDEDLEEYEKSGTKLFSLESRRQVREFDILAFSV
                     SFEQDYLNILEMLRLAGIHADKRERTGDDPLLVLGGICSFFNPEPISDFFDVVIVGEG
                     EEVAGEFIDMFKAHRGRDRAALLRALGTIPGVYVPEFYDVVYNADGTIRTRTKLESAA
                     PDRIEKQTTKDIGRLPAASVIFTPDTEFSDRYLAEITRGCGRHCRFCMAGYIYLPPRN
                     LAIDRAREQAEKADDLCGRIGLVGAAISDYPEIGELCNVITGGVSVSSLRADSVSEAL
                     IARLAKSGHKTISIAPEAGSERLRRVINKGISDGDTLRAADMVFGSGIPNLKLYFLIG
                     LPTETQEDVDGIISLAEQVREVQLRHARQRGRIGRITLSVNSFVPKPFTPFQWEPMET
                     VEALNKKLRTLEKAVRKIGNMNIIHDLPKWEHVQALLSRGDRRVGRLIRTAHDKGGDW
                     KGAARELGMDTDFYAARRRGFDEVLPWDFIDIGVRKEYLMNEYGRALEGKFTPPCKVG
                     SCKTCGVCR"
BASE COUNT          501 a          663 c          697 g          429 t
ORIGIN      
        1 gcggagcagg actcccatga atcaattttt gtcgttctga ggcgaagccg aagactctgg
       61 catttaactg cttagagctt gtcccgagtt tgccgaaggg ataaactcag cggagctcct
      121 cgtctttctt gacagttgcc agaaccttcg cttcgctcag ggtgacacaa gagaatcggt
      181 gcctcgggct cttcaatccg caggattgag ccggccgagt cccgcattgc atcatgatgc
      241 cccgcatagt ataatcccct catgtcccgg agactgcagg aaaagaccga cgcgctcatt
      301 gcggccgagc gcggggccgt gattaagcca cagggcgcgg acattgcggt cgccctggcc
      361 taccccaata cctaccacgt gggcatgtcg aacctcggcc tccatcagat ctacgccctc
      421 ttgaactccc gtcccgacac cgcctgcgag cgggtgttcc ttcctgacga tgaagacctc
      481 gaagagtacg agaagagcgg cacaaaactc ttcagcctcg agagcaggag gcaggtacgg
      541 gaattcgaca tcctcgcctt ctccgtgtcc tttgagcaag attacctgaa catactcgaa
      601 atgctccgtc ttgcggggat tcatgctgac aagcgcgaac gcaccggaga cgacccgctc
      661 ctcgtcctgg gcggcatctg ctccttcttc aaccccgagc cgatttctga ttttttcgac
      721 gtcgtgatcg tgggcgaggg cgaagaagtt gcgggagagt tcatcgatat gttcaaggca
      781 cacaggggcc gggaccgcgc ggcgctgctt cgcgccctgg gaactatccc cggggtgtac
      841 gtgcccgaat tttacgacgt cgtgtacaat gcggacggaa cgatcaggac ccgcacgaag
      901 cttgagtccg ctgccccgga ccggatcgag aagcagacga cgaaggacat cggtcgtctg
      961 cctgcggcat cggtcatctt cactccggac accgagttca gcgacagata tctggccgag
     1021 ataacccggg gctgcggcag gcactgcagg ttctgcatgg cgggatacat ctacctcccg
     1081 ccgcgaaatc tcgcaatcga cagggccaga gaacaggcgg aaaaggccga tgatctctgc
     1141 ggcaggatcg ggctcgtggg cgcggcgatc tcagactacc ccgagatcgg agagctgtgc
     1201 aacgtcatta caggcggggt ctcggtgtcc tcgctccgcg cggactcggt gagcgaggcg
     1261 ttgattgccc ggctggccaa aagcgggcac aagacgatct ccatcgcgcc ggaggcggga
     1321 tcggagaggt tgaggagggt catcaacaag gggatctccg acggggacac cctccgcgca
     1381 gccgacatgg tgttcgggag cggcattccg aacctcaagc tctatttcct catcggcctt
     1441 cccaccgaga cgcaggagga cgtggacggc atcatcagcc tggcagaaca ggtgcgggag
     1501 gtgcagctca ggcacgcccg gcagcggggc aggatcggcc ggatcacgct ctccgtgaac
     1561 tccttcgtgc ccaagccttt tacaccattc cagtgggagc ccatggagac ggtcgaggcc
     1621 ctgaacaaaa agctccgcac cctcgagaag gcggtcagga agatcgggaa catgaacatc
     1681 atccacgacc ttcccaaatg ggagcatgtc caggcgctcc tctcgcgcgg cgaccgccgg
     1741 gtcggcaggc tcatccggac cgcccacgat aaaggcggag actggaaggg agctgcgcgg
     1801 gaactcggga tggatacgga tttttacgcg gcccggagac gcggcttcga tgaagtcctg
     1861 ccctgggatt tcattgacat cggcgtgcga aaagaatatc ttatgaacga atacggacgg
     1921 gcgctcgaag gaaagtttac gccgccctgc aaagtcgggt catgcaagac gtgcggggtg
     1981 tgcaggtgac agcgcgtgct ttacttgaat gtcgatatta tatgcacgct gtcatccccg
     2041 aatggttaaa tcggggatcc attttgaatt ttgcttttag aaaagccaat ggattcccgc
     2101 tttcgcggga atgacggcga agacggaagt tctgaggatt acgaacgacg tatgacagcg
     2161 aacacaagtc aagtcggcat cagcatagcg attcccgccc ttgacggact gggaatcgta
     2221 cagtatgcct ttacgacccg gcaaaagggc ctcggcgcga gaacgcaggg ggtaagacag
     2281 cccggcgact
//
LOCUS       DASSLD010000204         2772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1492640, whole genome shotgun sequence.
ACCESSION   DASSLD010000204 DASSLD010000000
VERSION     DASSLD010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492640"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            439..840
                     /locus_tag="VFK23_03755"
     CDS             439..840
                     /locus_tag="VFK23_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318234.1"
                     /translation="MLEKRSAGDAIVSYCTKCKMGLDHVVVAMEGETVVKVKCRTCGS
                     AHKFRDPADVKKPRAAKKKEDAQKAAETLWENCLSSSRGKERTYDMGCKFRIGDVVLH
                     NTFGKGVVRKIYSNKCDVLFKDKERLMASAN"
     gene            968..2137
                     /locus_tag="VFK23_03760"
     CDS             968..2137
                     /locus_tag="VFK23_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET7318235.1"
                     /translation="MRKFPILITCPKGIPPFLKAELSALNFPVLSEHAAAVGTQGTFD
                     DAMRMNLRLRTAHRVLVELREFSANNADELYDRVAAINWEAYLPEDGYLCVTSSVENP
                     TIRDTRFATLKCKDAIVDRLKERCGRRPDSGPDRDKSVVHLYWKDSRVALYLDTSGEP
                     LSRRGYRKIPLAAPMQETLAAAVVIATGWDGTVAFVNPMCGSGTLAIEAVQIALGRAP
                     GLLRSNFGFMHLKGYDQAFWNELREEAKSEARKSVPGRVIATDISPDAVEAARKNAAT
                     AGVDHLIEFDVCDFAETPLPEGGVVVMNPAYGERMGEIARLEVTYKGIGDFLKQKCKG
                     YTGYVFTGNPGLAKKVSLKPKRSLQFFNSGIECRLLEYDLYEGTRKIKKPKPMPA"
     gene            complement(2174..>2772)
                     /locus_tag="VFK23_03765"
     CDS             complement(2174..>2772)
                     /locus_tag="VFK23_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7318236.1"
                     /translation="DVCSRIKALGRQCTAVRADVSSASDVSRLVETVRRELGTVTILV
                     NNAGIARTHTLNDITEQDWDSLMNVNLKAAFLVTQSVVSGMLTARWGRIINISSAAAQ
                     MGGVVGPHYAASKAGLIGLTHYYASHLAKEGITVNTIAPGPVRSEMSANLPQLNPSMV
                     PVGRFGEPEEVATVAVLLALNGYITGQTINVNGGRYMS"
BASE COUNT          645 a          803 c          808 g          516 t
ORIGIN      
        1 gaacaaaccg tcgcggataa gaatgtaacc ggaagcgaac aagaacccga gtatgtccgg
       61 cagcctggac agccccatga tgatgaggag atcgacaaat cccgcaaccg ctctggttgt
      121 taggtctgcc cgctgcaagg accctcctag ttagtgtaaa ccgattcata ctaacagaat
      181 gatacgtcga tttcaagcgc aaaaagggac ggttctgttg aaaagaaacg aatacccccg
      241 acccgcggcc cggtccgaca gtactgggtg caccacgtct ggaaaatgag ttagagcttt
      301 tttcttacga atggtgtata atgatatgtt tcaattttcg gccggggcgg ggcagatttg
      361 ccggtcccgt gaaccacaca cggtccgcga ttcctggttc aagcgcgcca aactcttaag
      421 cggaaaaagg agatttccat gctggaaaaa cgttcagccg gagacgcaat cgtatcctac
      481 tgcaccaagt gcaaaatggg ccttgatcat gtcgttgtgg ccatggaagg ggagacggtc
      541 gtcaaggtaa aatgcaggac ctgcggcagt gcgcacaaat tcagggaccc cgccgatgtg
      601 aaaaaaccga gggccgcgaa gaaaaaagaa gacgcgcaga aggcggccga aacgctctgg
      661 gaaaactgcc tgtcctcgtc aagagggaag gagcgcacct atgacatggg gtgcaagttc
      721 cgcatcggcg atgtcgtgct gcacaacacc ttcggcaagg gcgtcgtacg gaagatctac
      781 agcaacaaat gcgacgtact cttcaaagac aaagaacgcc tgatggcgtc ggcgaactga
      841 agcaaaatac gagggacgat ggacgaggga cgaaaaagaa aaagtccctt cgtccctcgt
      901 cctagtgagc gaagcgaacg gtcgtccctc gtccgtcgca tgctctccgc ggcaatcccc
      961 ccttcccatg cgcaaattcc ccatcctcat cacctgcccc aagggcatcc ccccctttct
     1021 caaagccgag ctttccgccc tgaactttcc ggtgttgtcc gagcatgcag ccgcggtcgg
     1081 gacccaggga acctttgacg acgccatgcg gatgaacctc cggcttcgca cggcccatcg
     1141 cgtcctcgtc gaactccggg agttcagtgc aaataacgcg gacgagctct atgaccgggt
     1201 cgcagcgata aactgggaag cgtaccttcc cgaagacggc tacctctgcg tgacctcgag
     1261 cgtggaaaac ccgaccatca gggacacgcg cttcgcgacc ctgaagtgca aggacgccat
     1321 cgtggaccgg ctcaaggagc ggtgcggaag gcggcccgat tcgggcccgg accgggacaa
     1381 aagcgtggtg catctctact ggaaggacag ccgcgtggcc ctctatctcg acacctcggg
     1441 cgaaccgctc tcccggcggg gctaccggaa aattcctctc gcggcaccca tgcaggagac
     1501 gctcgccgcc gcagtcgtca tcgcgacggg ctgggacggg accgtcgcct tcgtcaatcc
     1561 catgtgcgga agcggaacgc tcgccatcga ggcggtgcag atcgcccttg gccgcgcgcc
     1621 ggggcttttg cggagcaact tcggctttat gcacctgaag ggatatgatc aggctttctg
     1681 gaacgaactg cgtgaagagg cgaagagcga agcgcgaaaa agtgttccgg gacgagtcat
     1741 cgccacggac ataagccccg acgcggtaga ggcggctcga aaaaacgccg cgacagcggg
     1801 cgtggaccat cttatcgaat tcgacgtatg cgatttcgcg gagacgccgc tgcccgaagg
     1861 cggtgtcgtc gtcatgaacc ccgcttacgg cgagcgcatg ggtgagatcg cgagacttga
     1921 agtgacgtat aaaggaatcg gagactttct caagcagaag tgcaagggat acaccggata
     1981 cgtattcacg ggaaaccccg gccttgccaa aaaggtgagc ctcaagccga agcggagtct
     2041 tcagttcttc aacagcggca tcgagtgccg gcttctggag tacgatctgt acgaagggac
     2101 cagaaagatc aaaaaaccca agccaatgcc cgcctgagat cgatcttccg cttttgccgc
     2161 acattaattt cccctaactc atatatcttc cgccgttcac attgatggtc tgcccggtga
     2221 tataaccgtt caaggcaagc agcacggcga cggtggcgac ttcttccggc tctccaaagc
     2281 gtccgaccgg gaccatgctc gggttaagct gcgggaggtt cgccgacatt tcagaccgga
     2341 ccggtcccgg ggcgatggta ttcacggtaa tcccctcctt tgcgaggtgc gaggcatagt
     2401 agtgtgtgag cccgatgagc cccgcttttg atgccgcata atgggggccg acaactcctc
     2461 ccatctgggc cgcggccgac gatatgttga tgattctgcc ccagcgcgcg gtcaacatgc
     2521 cggaaactac cgactgggtc acgagaaagg ccgctttgag gttgacgttc atgagggagt
     2581 cccagtcctg ctccgtgatg tcattgaggg tatgagtccg cgcaatgccc gcattgttta
     2641 ccaggatggt cacggttccc agttcgcgcc ggacggtttc cacgagccgc gagacgtccg
     2701 atgccgacga gacgtcagcc cgcacggccg tgcattgtct gccgagcgcc ttgattctgc
     2761 tgcatacatc ct
//
LOCUS       DASSLD010000205         4101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_822162,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000205 DASSLD010000000
VERSION     DASSLD010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822162"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /gene="hisI"
                     /locus_tag="VFK23_03770"
     CDS             <1..427
                     /gene="hisI"
                     /locus_tag="VFK23_03770"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HET7318237.1"
                     /translation="KNRSLLRGRFFYARLWTFPADCVKTSFMIIPGNLKYDEKGLIPA
                     VIQDWKTGEVLMVAYMNAESLKRTVETSLTWFWSRSRQKFWQKGETSGNVQRVKDILY
                     DCDQDTLLLKVEQTGPACHTGSRCCFFRSFSDVAQGEKK"
     gene            424..777
                     /locus_tag="VFK23_03775"
     CDS             424..777
                     /locus_tag="VFK23_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HET7318238.1"
                     /translation="MSLDPNCIFCRIIEKKIPAKIVYEDDLVTAFEDTNPQAPVHTLV
                     VPKKHIPDVHSMTEADRELVGHLFFAAQKVADDRGLDSKGYRLVINNGRGAGQTVFHI
                     HLHLLSGRRFTWPPG"
     gene            1185..1553
                     /locus_tag="VFK23_03780"
     CDS             1185..1553
                     /locus_tag="VFK23_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HET7318239.1"
                     /translation="MENKEFVFILTHSYDAIERSAGALQLATNMAAFDAKIDFFLMNE
                     GVHLARKGFAESVSWQHGFSPVAELMKSLIEDFDCKFYICASCVKPYGLEGAELIKNS
                     EVKPGSFLGEMLMKRQGLTF"
     gene            1617..2192
                     /locus_tag="VFK23_03785"
     CDS             1617..2192
                     /locus_tag="VFK23_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7318240.1"
                     /translation="MKNIANFLFEAGMLKRTPRSGFQFLGSGAESVAEHIFRTSYIAY
                     ALGKQAVDVDVDRMIKMCLFHDLPEARTGDLNYVNKKYVEANEKKAVEDLARTLPFGD
                     ELRELILEFIEGKTEEAKLARDADQLEMILALKEYKDLGNKYADEWLDFSLKRLQTDA
                     ARELATTILETDSSLWWFSDKGDWWVSGGKR"
     gene            complement(2133..2807)
                     /locus_tag="VFK23_03790"
     CDS             complement(2133..2807)
                     /locus_tag="VFK23_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HET7318241.1"
                     /translation="MTKNFTGPVDAILLIGPTGSGKSPLGDILESRGFLGRRSHHLDF
                     GSELRSAVSGIGASSYTHEEQEFIGGVLERGLLLENEHFVLAKKIIGLFLERSRFRRG
                     DVMVLNGIPRHEGQARDVASIATVHSLVVLDCSVEAVLLRIRENTGGDRTGRADDDHA
                     LVENKLRIFNERTAPLIEHYRKAGSRIYRIDINEKTTTASAYHRLSTLAAADPPITLV
                     TEPPQR"
     gene            complement(2804..3241)
                     /locus_tag="VFK23_03795"
     CDS             complement(2804..3241)
                     /locus_tag="VFK23_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7318242.1"
                     /translation="MKKGIDYIGVGVGAVIFDSDGRVFLAKRGREARNESGKWEFPGG
                     GVEYGETLEQAIMREVREEYGIEIEVLDLLDVVNHLIPEERQHWVSPTFLCRIASGTP
                     CIREPHKCDDIGWFAIDEIPEEKLSLASKKSLSSLRKRNELKR"
     gene            3389..3526
                     /gene="rpmH"
                     /locus_tag="VFK23_03800"
     CDS             3389..3526
                     /gene="rpmH"
                     /locus_tag="VFK23_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET7318243.1"
                     /translation="MSETTAQKSNLKQKRAQGFRKRMSSPTGREVINRRRAKGRKRLA
                     V"
     gene            3547..3915
                     /gene="rnpA"
                     /locus_tag="VFK23_03805"
     CDS             3547..3915
                     /gene="rnpA"
                     /locus_tag="VFK23_03805"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET7318244.1"
                     /translation="MPYTYRKSERIRKNTEFISTMKGKRLSFDGLSLFYTRNDIGNFR
                     VGISISKKLANAAKRNRLRRRIRSCVMKALAGHSLGYDLVFVARREISAAEYDTIMKT
                     VETVLHRTALRGQRPEGTRP"
     gene            3912..>4101
                     /gene="yidD"
                     /locus_tag="VFK23_03810"
     CDS             3912..>4101
                     /gene="yidD"
                     /locus_tag="VFK23_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET7318245.1"
                     /translation="MNRFFIAIIRRYRRHISPFLPQACRFTPSCSEYAIEAFEKKSAP
                     RAIWLTALRIAKCHPFHPG"
BASE COUNT          954 a         1106 c         1112 g          929 t
ORIGIN      
        1 gaaaaaccgg tcccttctgc ggggccggtt tttttatgct cgtctctgga catttcccgc
       61 cgattgtgtt aaaaccagtt tcatgataat tcccggaaac ctcaagtacg atgaaaaagg
      121 cctcatcccc gccgtgatcc aggactggaa aacgggcgag gtcctgatgg tggcctatat
      181 gaacgccgag tcgctcaagc ggaccgtcga gactagtctc acctggttct ggagccgctc
      241 gcgccagaag ttctggcaga agggcgagac ctcgggaaac gtccagcgcg tgaaggacat
      301 cctgtatgac tgcgatcagg atacgctgct cctcaaggtg gagcagaccg gccctgcctg
      361 tcatacggga tcgcgatgct gcttcttcag gagcttttcc gacgttgctc agggggaaaa
      421 aaagtgagcc tcgatcccaa ctgcatcttc tgcaggatca tcgaaaaaaa aatacccgca
      481 aaaatcgtgt atgaagacga tcttgttacg gccttcgaag acacaaaccc gcaggccccg
      541 gttcacacgc tggtcgttcc caagaagcat attcccgatg ttcacagcat gaccgaggca
      601 gaccgggaac tcgtcggaca cctttttttc gcggcgcaga aggtcgcaga tgaccggggc
      661 cttgattcaa agggctaccg cctggtcatc aacaacggcc gcggtgcggg ccagaccgtg
      721 ttccacatcc accttcacct tctgtccgga cgccgtttca cctggccccc gggataataa
      781 caccggtctg tgggttgttg aaggacaaga tgtggaaaac gcggatatgc cggttgtcaa
      841 cagggctgtc ctcagaaatt aacaaacaac cgcatcgctt cttcttttct tttttgtttt
      901 tcctttcaac cggttctccg gtgttccaac atattcacaa cctctactat tacgactact
      961 aattttatta atatatatcc ataatagatt cttactgacg atacctgtcg ataaccaccg
     1021 ttgcaatacg ctgttcgatt ccggattaac aatgcaataa aaatttttta ttagagaagc
     1081 ggtggcaagc atggtatctt tacgtcatca aaagctctct ctccgtcatt tccctgaatc
     1141 ggggatgacg tcagagacac gtcttgaacc atagggagga gtatatggaa aacaaggagt
     1201 ttgtttttat tctgacccat tcgtatgatg cgatcgaacg ttcggcaggg gcgctccagc
     1261 ttgcaaccaa catggcggcc tttgacgcga agattgactt ctttctgatg aacgaaggag
     1321 tccacctcgc gcgcaagggg ttcgccgagt ccgtatcgtg gcagcacggt ttttctcctg
     1381 ttgcggagct tatgaaaagc ctgatcgagg atttcgattg caagttctac atatgcgcgt
     1441 cctgcgtaaa accctacggg ctggaaggcg ctgaattaat caagaactcc gaagtaaagc
     1501 cgggctcctt tctcggcgag atgttgatga agaggcaggg gctgacgttc tgacggcgtt
     1561 tccctccgca atgaagaata tttacagatt ccgcaaaaca tggtataaac cctgccatga
     1621 aaaatatcgc gaatttcctg tttgaggccg gcatgctcaa acgcacgccg cggagcggtt
     1681 ttcagttcct cggttccggc gcggagtccg tggcggaaca tatcttccgg acctcgtata
     1741 tcgcctacgc cctcgggaaa caggctgtgg acgttgatgt cgaccgcatg atcaagatgt
     1801 gcctgttcca cgacctgccg gaggcgagga ccggagacct caattatgtg aacaagaaat
     1861 atgtcgaggc gaacgagaaa aaagcggtcg aagacctcgc gcggacactg ccgttcggcg
     1921 acgagctccg ggaattgatc ctggaattca ttgagggaaa gacagaagag gcaaaactgg
     1981 cgcgcgacgc ggaccagctt gagatgatcc tcgctctcaa ggaatataaa gacctcggca
     2041 acaaatacgc agatgagtgg ctggattttt cgctcaagcg gctgcaaacc gatgccgcgc
     2101 gcgagctggc aacgaccatt cttgaaaccg attcatcgct gtggtggttc agtgacaagg
     2161 gtgattggtg ggtcagcggc ggcaagcgtt gagagccggt gataggcgga cgcggtcgtc
     2221 gttttctcgt tgatatcgat gcggtaaatg cggcttcccg cctttcggta gtgttcgatg
     2281 agcggcgcgg tccgctcgtt gaaaatcctg agtttgtttt caacaagcgc gtggtcgtcg
     2341 tccgcgcgcc cggtcctgtc cccgccggta ttttccctga tcctcaggag aacggcttca
     2401 acggagcagt caagaaccac gagcgagtgg accgtggcga tcgatgcgac gtctcgtgcc
     2461 tgcccttcgt gacggggtat gccgttcagg accatcacat cgccacggcg gaagcgggag
     2521 cgttcgagga aaagaccgat gatcttcttc gcgagaacga agtgctcatt ctcgagaagc
     2581 aggccccgct cgagcacacc cccgatgaat tcctgctcct cgtgcgtata ggaagaggcg
     2641 ccgatccccg agacagcgga gcggagctcg gagccgaagt ccagatgatg ggacctcctg
     2701 ccgagaaaac cgcgcgattc gaggatatcg ccgagcgggc tttttccgga gccggtcggc
     2761 ccgatgagga ggattgcatc gacgggaccg gtaaaattct tcgtcatcgt ttaagctcgt
     2821 ttctctttct caggctgctc aaactttttt tcgacgcgag ggaaagcttc tcctcgggga
     2881 tttcatcgat cgcaaaccag ccgatatcgt cgcatttgtg cggctcccgg atgcaggggg
     2941 ttccgctcgc aatcctgcac aggaatgtcg gtgaaaccca gtgctgccgt tcctccggaa
     3001 tgaggtggtt tacgacgtcg aggaggtcca ggacctcgat ctcgatgccg tattcttccc
     3061 tgacctcgcg catgatcgcc tgttcgaggg tttcgccata ctcgacgccg cccccgggga
     3121 attcccattt gcccgattcg ttcctcgcct ccctgcctcg tttggcgaga aaaacacggc
     3181 catcggagtc gaagatcacg gcgcctactc ccacgccgat ataatcgatg ccttttttca
     3241 taatctgttt tgtttattcg ctgcaatgta ccaaaaatga cggacaaagt caaaaccctg
     3301 tttccacatg gttttttgct tgacattgtt tcgttttagc ttataatacc cccctgttcg
     3361 taatatccat aacgaaggag cagtcacgat gtcggagaca acagcacaaa aaagcaatct
     3421 caagcagaag cgggcgcagg gcttccgcaa gcggatgagc tcgcccacag gcagggaggt
     3481 catcaaccgc cgaagggcca agggaagaaa gcgtctcgcc gtatagcaca ccgcggtaaa
     3541 ggaccgatgc cgtacacata ccgcaagagc gaacggatac gaaaaaatac cgaatttatc
     3601 tctacgatga aaggaaaaag actgtcattc gacggtcttt ctctttttta tacccggaac
     3661 gacatcggga atttcagggt cgggatttcc atcagcaaga agctggcaaa cgcggccaag
     3721 agaaacaggt tgagacgtag gatacgaagc tgcgtcatga aggccctggc gggacactcg
     3781 cttgggtatg acctggtctt cgtggcgcga cgggagatct cggctgcaga atatgatacg
     3841 atcatgaaga ccgtcgagac cgttcttcac aggacggccc ttcgaggtca gcgaccggaa
     3901 ggcaccagac cgtgaaccgt tttttcatag ccatcatccg ccggtaccgg cgtcatattt
     3961 cgcctttcct gccgcaagcc tgccggttca ccccctcgtg ttctgaatac gctatcgagg
     4021 cctttgagaa gaagagcgcg ccgcgggcga tctggttgac tgccctgcgg atcgcgaagt
     4081 gccacccctt tcatcccggc g
//
LOCUS       DASSLD010000206         2835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_921164,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000206 DASSLD010000000
VERSION     DASSLD010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921164"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VFK23_03815"
     CDS             complement(<1..399)
                     /locus_tag="VFK23_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HET7318246.1"
                     /translation="MVIRISDIPPEGLTLELAHDLDLFDQGTATTAFQAVIGIRPASR
                     GILRVTGKIHSSPVLECSRCLEKFTFAIDTELDIELAPVSALGAAAEHELAGGELDTE
                     FYEGDVIEPVDLIKEQLLISIPMVPLHSPEC"
     gene            complement(532..2016)
                     /gene="bioA"
                     /locus_tag="VFK23_03820"
     CDS             complement(532..2016)
                     /gene="bioA"
                     /locus_tag="VFK23_03820"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HET7318247.1"
                     /translation="MHYNSDREKQLAADSSGELLLLLRAMNEREERFLPSPRMKRQAA
                     AENLEKLDKKYIWHPFTQMQDWEKDPQIIIESGAGSTLTDISGKTYLDGVSSLWVTVH
                     GHRKKEIDRAVGAQLGRVAHSTLLGLSNVPAVLLAEKLVRTAPKGLTRVFYSDSGSTA
                     VEIALKIAFQYWQQKGPEHQKKTGFLSLAGAYHGDTIGSVSVGGIDLFHAIYKPLLFQ
                     TRKIPSPHCYRCTYGLTDPDCQKTCFVHAERTIQKYASVTAALIIEPLVQGAAGMLTH
                     PPGFLKKIRELCTKYKILMIADEVATGFGRTGKMFACEHEGVSPDILCVAKGLTGGYL
                     PLAATLATEAVYQGFLGEYHEFRTFFHGHTYTGNPLACAAAMASMNLFEREKTLTKLR
                     PKIVLLAKELKRFHELKHVGEVRQRGFMVGVELVKNRETKEPYPLAEKIGIRVSTECR
                     KLGLLIRPLGNVIVLMPPLSVSLKDLKRMTDIIYDAVRRVTEGV"
     gene            2150..>2835
                     /locus_tag="VFK23_03825"
     CDS             2150..>2835
                     /locus_tag="VFK23_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015902201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7318248.1"
                     /translation="MLTKKKLYVSAAVILVLGVFVGLILSSHVGIMSTLPAKSQISSK
                     TIDILTQLSEAQSEVAAVATPSVVNISTTRVIKSREEAPFDLFDDPFFKRFFGDQFPH
                     PNIPKEHKEQSLGSGVIVSDDGYIVTNNHVIEKAQEIKVLLLNKKDYTAKLIGADPKT
                     DIAVIKIDAKDLPALSWGDSNKLKVGEIVFAIGNPFGLNQTITMGIISAVGRANVGIA
                     DYEDFIQTDAA"
BASE COUNT          583 a          789 c          824 g          639 t
ORIGIN      
        1 gcattcggga ctatgaagcg ggaccatggg gatggagatg agcagctgtt cttttatgag
       61 atcgacaggc tcgattacgt ctccctcgta aaactccgta tcaagttcgc caccagccaa
      121 ttcgtgctcc gcagcggcgc cgagggcgct caccggggcg agttcgatat ccagttccgt
      181 gtcgatggca aaggtaaatt tttccagaca gcggctgcat tcaagcacgg gggaagaatg
      241 tatttttccg gttacgcgca ggatgccgcg cgacgcaggc ctgatgccga tgactgcctg
      301 aaacgctgtc gttgccgtgc cctggtcgaa gagatcgaga tcatgagcca attccagggt
      361 gagcccctct ggcggtatgt cgctgattct gatcaccatt tctcctgctc cgggggctgc
      421 aagtgacgaa ttatatagag aagctcccga tttgtcaaga aaaaataccg gtgagacagc
      481 atcgcggcat caaagggggg gccggaacgg agcacgggga ggaagcccgt ttcacacgcc
      541 ttccgttacg cgccgaacgg cgtcatagat gatgtcggtc atgcgcttca ggtccttgag
      601 ggaaaccgac agcggcggca taagcacgat cacgttgccg agcggccgga tgagaagtcc
      661 tagtttgcgg cattcagtgg acacgcggat gccgattttt tcggccaggg gatagggctc
      721 cttcgtctct ctgttcttca ccagttcgac gcctaccatg aacccccgct gtctcacctc
      781 tcccacgtgc ttcagttcat gaaaccgttt cagctctttt gcaagcaaca ctatcttggg
      841 acgaagcttc gtgagcgttt tttcccgctc aaaaaggttc atgctggcca tggcagctgc
      901 gcaggcaagg gggttcccgg tataggtatg gccgtggaaa aaggtcctga attcgtgata
      961 ctcgccgagg aagccctggt agacggcctc ggtcgccagc gtagccgcaa gaggcaggta
     1021 gccgccggtg agtcccttgg caacgcagag aatgtccggg gagacgcctt catgctcgca
     1081 ggcgaacatt ttcccggtcc tgccgaaacc cgtcgcgacc tcgtccgcga tcatcagaat
     1141 cttgtatttc gtgcacagtt cacggatctt tttcaggaat cccggcgggt gcgtgagcat
     1201 cccggcggcg ccctgcacca ggggttcgat aatcagggct gccgtcacgg aggcgtactt
     1261 ctggatggtc ctctcggcgt gcacgaaaca ggtcttctgg caatcggggt cggtcagccc
     1321 ataggtgcac cggtagcaat ggggagacgg gattttgcgg gtctgaaaca ggagcggctt
     1381 gtagatcgca tgaaagaggt cgatcccgcc cacgctgacg gacccgatcg tatcgccgtg
     1441 atacgccccg gcgagcgaga ggaacccggt ttttttctga tgctcggggc ccttctgctg
     1501 ccagtattga aaggcgatct tgagcgcgat ctcgacggcg gtggaaccac tgtcggagta
     1561 aaacaccctg gtaagcccct tcggcgcagt tctgaccagt ttctcggcga gaagaaccgc
     1621 cggaacgttg gaaagcccga gaagggtgga atgggcgacc ctgccgagct gcgcgccgac
     1681 ggccctgtcg atttcttttt tccggtggcc gtgaaccgtc acccagagcg aggacactcc
     1741 gtcgagatag gttttcccgc tgatgtcggt gagcgtgctt cctgccccgc tttcgatgat
     1801 gatctgcgga tctttttccc agtcctgcat ctgggtaaag ggatgccaga tgtatttctt
     1861 gtccagtttt tccagattct cggcggcggc ctgtcttttc atgcgtggcg acggcaagaa
     1921 acgctcctcc ctctcgttca tggcccgcag cagcaaaagc agttcccctg agctgtcagc
     1981 tgcgagctgt ttttcccggt cgctattata gtgcacaggt gttttttgtc aatcataaaa
     2041 aaggcttgaa aagaatcgtt tctttccggt ataatggctg gatgatggga actccttgct
     2101 ggtacccgtc acagtcctgt tccagccact ccggaggtcg tttgcaatca tgctcaccaa
     2161 gaagaagttg tatgtttcgg cggcggtcat tctcgttctc ggcgttttcg tcggacttat
     2221 tctttcctcc catgtcggca tcatgtcgac gcttcccgcc aagagccaga tatcgtccaa
     2281 aacgatcgat atcctgacgc agctttccga agcccagtcc gaggtcgccg ccgtcgcaac
     2341 gccttccgtg gtcaatatat ccacgacgcg ggtgatcaag tcacgggagg aagcgccgtt
     2401 cgatcttttt gacgaccctt tcttcaagcg gtttttcggc gatcagttcc cgcaccccaa
     2461 catcccgaaa gagcacaagg agcagagcct gggttcgggc gtgatcgtct ctgatgacgg
     2521 ctacatcgtc acaaacaacc acgtgatcga gaaggcgcag gaaatcaagg tgctcctgct
     2581 gaataagaag gactatacgg cgaagctgat cggtgcggac ccgaagaccg acatcgccgt
     2641 catcaagatc gacgccaagg atttaccggc gctctcctgg ggagattcca acaagctcaa
     2701 agtgggcgag atcgtgtttg cgatcggtaa ccccttcggt ttgaaccaga ccattacgat
     2761 gggcatcatc agcgcggtcg ggcgggccaa cgtgggcatt gccgattatg aggacttcat
     2821 ccagacggac gcggc
//
LOCUS       DASSLD010000207         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_148853,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000207 DASSLD010000000
VERSION     DASSLD010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148853"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..446)
                     /gene="rpsU"
                     /locus_tag="VFK23_03830"
     CDS             complement(174..446)
                     /gene="rpsU"
                     /locus_tag="VFK23_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET7318249.1"
                     /translation="MEVRVQSRDIEKALRALKKKLQLDGVFGELKKRRFYEKPSVKKK
                     KKQLEAQKRKRKLLKRSRPSDHSPRPGSGYATRPLQRTAVPEKVGT"
     gene            complement(437..760)
                     /locus_tag="VFK23_03835"
     CDS             complement(437..760)
                     /locus_tag="VFK23_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET7318250.1"
                     /translation="MAKKLYVGNLSYSTHDEDLREAFSKIGEVLSVTLITDQATGRSK
                     GFGFVEMATDEDADKAIAALNGTSFMERTITVNEARPKTERGRPSGGRGDRGGFSGGR
                     NRSWR"
     gene            complement(1168..>2256)
                     /locus_tag="VFK23_03840"
     CDS             complement(1168..>2256)
                     /locus_tag="VFK23_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET7318251.1"
                     /translation="RISGLPLTPYYSASKLRWIRENIESSKQPDAVFGPLSSFLVRQL
                     TGNKSDVVDHTNAARTQLMNIRTLSWDEELLGIFGLTGVGLPKIVPSEYDFGKMSTPA
                     GEVPLLACCGDQQAAMIGLGVLEKGDSGINLGTGGFLMTNTGGELVPAEGIMASVHYS
                     DGKEVRYLLEGSVNAAGDALEWMRMNLGLFNDYGEVDDLCWKAATDTVAFVGLNGTGA
                     PHWEQDISSAFHGLGPQSTSADLVRAVVEGFVFFIRDIAEEIRKHGIEPRNFVLAGGL
                     SSLSYLSQIQADILKTDVRVSASREASALGAAFLAGMRHGVWNSDDIRRMTAQGETVN
                     GEANPGLERRYKKWKELHRMTRELDNIS"
BASE COUNT          435 a          686 c          608 g          527 t
ORIGIN      
        1 agtatagtga aacggttggg agaggtcaat taaaaaaaat aatatcagag cggtactttt
       61 tattgtcgcg cggattgatt accacttgaa tgccgacatg gcaactgtaa gggacagaaa
      121 acagctattc ctgtattgag agttcatcgt cggccgtgct gcgtgtcggg ccgttacgtt
      181 cccacttttt ctggcacggc tgttcgctgc agcggacgcg ttgcgtaccc gcttcccggc
      241 ctcggcgagt gatcggacgg gcgcgagcgc ttgaggagct ttcgtttccg cttctgcgcc
      301 tccaactgtt tcttcttctt tttcaccgag ggcttttcgt aaaagcgccg cttcttcagc
      361 tccccgaaca ctccgtcgag ctggagtttc tttttcaggg cccgcagggc cttttcgatg
      421 tcccgggact gaacccttac ctccaagacc ggttccttcc gccgctgaac ccgccgcggt
      481 cacccctgcc gccgctcggg cgcccgcgct ccgtcttcgg acgggcctcg ttcaccgtga
      541 tcgtccgctc cataaacgac gtaccgttca gggccgcgat cgccttgtcg gcgtcctcat
      601 ccgtcgccat ctccacaaaa ccgaacccct tggacctgcc ggtcgcctga tcggtgatca
      661 gggtgacgga caggacctcg ccgatcttcg agaacgcctc gcgcaggtcc tcatcgtggg
      721 tcgagtacga cagattgcct acataaagtt tcttcgccat aactcctcct gtataaattc
      781 aagttcgcgc tgccggtcac cctgcgcggc gcgtttactt cccaatcgcg agaacgcgca
      841 gcatcccgcg ggacttggta aagccgcatc gtttgccaac tatgcttgct gtcgcttttt
      901 gagacgagaa aaggagagag caggaagagt cagtaatcga tgatgaggcg ccgacgacgg
      961 cggcctgtct tcggaacctg tcgcacccca ttgtttattt attatacctg tttcagcgtc
     1021 agatgcaagt attttccgat caactcgcca ggaaagaatc catccacgtt gttcaggaca
     1081 gcggttaaga aggcctcctc gcatcaggca attagtttaa ctcagaggag atattctcta
     1141 atgacgatca tcgggtggtt accggcctta cgaaatatta tccagctctc tcgtcatccg
     1201 gtgcagttcc ttccactttt tgtatcgtcg ttcaagtcca gggttcgctt cgccgttcac
     1261 cgtctcaccc tgggccgtca ttctcctgat gtcatcagaa ttccatacgc cgtgccgcat
     1321 gccggccaga aatgccgcgc ccagggcgga tgcctccctg gaggcgctta cgcgaacatc
     1381 cgttttgagg atgtctgcct ggatctggct caaatagctg agtgacgata accctcccgc
     1441 aagcacgaag ttccgcggtt cgattccgtg cttcctgatc tcctcggcga tgtccctgat
     1501 aaaaaagacg aagccctcaa cgacggcgcg caccaaatct gccgacgtgc tttgaggccc
     1561 cagaccgtga aacgcgctcg agatatcctg ttcccagtgc ggcgcgccgg tgccgttcag
     1621 gccgacgaac gccaccgtgt cggtcgcggc cttccagcag agatcgtcca cctctccgta
     1681 atcattgaag agcccgaggt tcattctcat ccattcgagg gcgtcacccg cggcgtttac
     1741 gctcccttcg aggagatagc gcacttcttt cccgtccgag taatgtaccg aggccatgat
     1801 gccctctgcc ggcacgagtt cgccgcccgt gttcgtcatc agaaacccgc ccgttccgag
     1861 gttgattccc gagtctcctt tttcgagcac gccgagcccg atcatggcag cctgctggtc
     1921 gccgcagcag gcgagaaggg ggacctctcc cgccggggtg ctcattttcc cgaagtcgta
     1981 ctcagaggga acgatcttcg gaagccctac accggtgaga ccgaagatcc cgagcagttc
     2041 ctcgtcccag gaaagggtcc tgatattcat gagctgggta cgggcggcgt tcgtgtggtc
     2101 gacgacgtct gacttgtttc cggtgagctg ccggaccagg aagctggaga gcggcccgaa
     2161 tactgcgtcc ggttgtttcg aggactcaat attttccctg atccagcgaa gcttggaagc
     2221 cgaatagtag ggagtgagcg gaagaccgga tatccg
//
LOCUS       DASSLD010000208         4204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_300389,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000208 DASSLD010000000
VERSION     DASSLD010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300389"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VFK23_03845"
     CDS             complement(<1..863)
                     /locus_tag="VFK23_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HET7318252.1"
                     /translation="MERYVCIHGHFYQPPRENPWLEAIELQDSAHPYHDWNERITAEC
                     YAPNSAARLLDGEQKIIDIVSNYEKISFNFGPTLLSWMEKATPDAYRAILDADRRSGE
                     QFSGHGSAIAQAYNHIIMPLANSRDKRTQAIWGIRDFERRFGRSPEGMWLPETAVDVE
                     TLDVLAGAGTKFTILAPHQAAHIRESGREDWLDVSGGRVDPTRPYLCRLLSGREIVLF
                     FYDGPISRAVAFERLLTRGEDFAGRLMGGFSNERTWPQILHITTDGESYGHHHRFGDM
                     ALAYALHDIEAG"
     gene            complement(863..2695)
                     /gene="treZ"
                     /locus_tag="VFK23_03850"
     CDS             complement(863..2695)
                     /gene="treZ"
                     /locus_tag="VFK23_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HET7318253.1"
                     /translation="MKLGAHLQDEICSFLVWAPFSNEVEVKIVFPEEVTVPLEKDDRG
                     YWSGMLERASSGMRYIFRLDGGKERPDPASRFQPLGVHGPSQIVDPDEFAWQDNGWTG
                     VVLDDYVIYELHVGAFTREGTFESVVPHLDYLRDLGITALELMPVAQFPGTRNWGYDG
                     VYPFAVQNSYGGPTGLKKLVNACHERGLAVILDVVYNHLGPEGNYLWDFGPYFTDKYK
                     TPWGDAINFDGPHSDEVRRYFIENALSWITDFHIDALRIDAIDGIFDFSAKHFLEELG
                     DAVHARAGELGRRVYVIAESDLNDVRVINPKERGGYGLDAQWNDDFHHALHTLITEER
                     GGYYEDFGDISHLEKAYREGFVYSGEYSVFRKRRHGSSSRHLSARHFVIFSQNHDQVG
                     NRMRGERLSSLASFEALKLAAAAVLLSPNIPLLFMGEEYGEDAPFMYFVSHSDYDLIE
                     AVRKGRKEEFRAFSWGGEHPDPQSEETFLASRIDPAKGKGGRYNALLRYYGMLLTLRR
                     TIPALKEPDRDRLEVSVIPDTGVITLHRWDRTRTSRVYCLFNFKKNSVEAFLAAEGTW
                     KRVLDSADSSWNGPGSLFPEQLREQGRGTVRAQSVVVFVKEEDR"
     gene            complement(2692..3921)
                     /locus_tag="VFK23_03855"
     CDS             complement(2692..3921)
                     /locus_tag="VFK23_03855"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET7318254.1"
                     /translation="MAHPKVLAFVLAGGKGERLAPLTDLRSKPSVPFGGRFRIADFVL
                     SNLVNSHIYSVYLLVQYKSQSLIEHVRQNWVLSPVIRDHFVAVVPPQMRMGPEWFQGT
                     ADAVYQNVNLIEDQDPELVIVFGADHIYRMDIRQMIDFHLEKGAEVTLAARPVPIGQA
                     ADFGVILTDRERRITGFQEKPERPAPMPDDPGRSYVSMGNYIFNRDVLVDSLVKAQKE
                     GRHDFGAHIIPSLVGPGRVYAYDFATNIIPDAKSYEEKGYWRDVGTIPSFYQAHMDML
                     GRTPLLDLHNRSWPIQPAGYKGPAATILRGEIQNSIISEGVLVEDARIVNSVIRRDVV
                     IEDGAVVENSIIMDHTIVGKGCRLKGVIADKFNVFTEGLVLGDDVEKDRLYSGIHIDP
                     SGIRIIPKAGRTAGQGR"
BASE COUNT          791 a         1273 c         1251 g          889 t
ORIGIN      
        1 ccggcttcga tgtcgtgaag ggcgtaggcc agggccatat cgccgaaccg gtggtgatga
       61 ccgtaggatt cgccgtccgt cgtgatgtga aggatctggg gccacgtcct ctcattggaa
      121 aaaccgccca tgagacggcc cgcaaaatcc tcacccctgg tgagcagccg ctcgaacgcc
      181 acggcgcggg atatggggcc gtcgtagaag aaaaggacga tctcccttcc cgacagcagc
      241 ctgcacagat agggccgcgt ggggtcgacc ctgccgccgc tgacatcgag ccaatcttcc
      301 ctgccggact cacggatgtg cgcggcttgg tgaggggcga ggatggtaaa ctttgtaccg
      361 gccccggcga ggacatccag agtctccacg tccaccgccg tctcgggcag ccacatccct
      421 tccgggctgc gtccgaaccg gcgctcgaag tccctgatgc cccatattgc ctgcgtccgc
      481 ttgtcgcggc tgttcgccag gggcatgatg atatgattgt atgcctgggc gatggcgctt
      541 ccgtggccgg agaactgctc cccgctcctc cggtccgcat caaggatcgc tcggtaggcg
      601 tcgggcgtcg ctttctccat ccacgacagg agcgtggggc cgaaattgaa gctgatcttc
      661 tcgtaattgc tcacgatgtc gatgattttc tgttccccgt caagaagccg cgcagccgag
      721 ttcggtgcgt agcattccgc cgtgatgcgt tcgttccagt catggtaggg atgggcggag
      781 tcctggagct cgattgcttc gagccagggg ttctcccgcg gaggctgata gaaatggccg
      841 tggatgcaga cgtatcgctc cattaccggt cttcctcttt gacgaataca accacgctct
      901 gcgcgcggac ggttccgcgg ccctgttcac ggagctgttc aggaaacaag gagcccggtc
      961 cgttccagga actatccgcg gaatcaagca ccctcttcca ggtcccctcc gccgccagaa
     1021 aggcctctac gctgttcttt ttgaaattga agagacaata gacacggctg gtcctggtcc
     1081 tgtcccatcg atgcagggtg atgactcccg tgtccgggat gacgcttacc tcgaggcggt
     1141 cacggtccgg ttccttgagc gcgggtattg tccgtcgcag cgtcaagagc ataccgtagt
     1201 atcggagaag cgcgttatac cggccgccct tgccctttgc cgggtcgatc ctggacgcaa
     1261 ggaaggtctc ttcgctctgc gggtcgggat gctcgccgcc ccaggagaag gccctgaatt
     1321 cctccttcct tcccttgcgg accgcctcga tgagatcgta gtcggagtgg ctcacgaaat
     1381 acataaacgg cgcgtcttct ccgtactcct ctcccatgaa caggagggga atgttgggag
     1441 aaagaagaac tgccgctgcg gcaagcttca aggcctcaaa ggaagcgagc gaggacagcc
     1501 gttcgccgcg catgcggttc ccgacctggt cgtggttctg ggaaaaaatg acaaagtgcc
     1561 gcgcggacag gtgcctggag gaactcccgt gccttcgttt tctgaaaacc gagtactcgc
     1621 cggaatatac gaaaccctcg cggtacgcct tctccagatg ggagatatcg ccgaagtcct
     1681 cgtagtatcc gccccgctct tccgttatga gcgtatggag ggcgtgatgg aaatcatcgt
     1741 tccactgggc atcgaggccg tagccgcccc gctccttcgg gttgatgacc cgtacatcgt
     1801 tcaggtcgct ctcggctatg acgtaaacgc gccggcccag ttcccctgcc ctggcatgaa
     1861 ccgcgtcgcc gagttcctcc aggaaatgtt tcgcgctgaa atcgaagatg ccgtcaatgg
     1921 cgtcgatgcg aagtgcgtca atatggaaat cggtaatcca ggaaagcgcg ttttcgatga
     1981 aataacgcct cacttcgtca ctatgcgggc cgtcgaagtt tatcgcgtcc ccccagggcg
     2041 ttttgtattt atcggtgaaa taggggccga aatcccagag ataattcccc tcgggaccga
     2101 ggtggttata gaccacgtcg aggattacgg caaggccccg ctcatgacag gcgttcacga
     2161 gctttttaag gcccgttggc ccgccgtagg agttctgtac ggcgaaggga tagacgccgt
     2221 catagcccca gtttctcgtc cccgggaatt gcgcgaccgg catgagttcc agggccgtga
     2281 taccgaggtc cctgagataa tcaaggtgcg ggacaaccga ctcgaacgtt ccctccctgg
     2341 tgaaggcgcc gacgtgaagt tcgtagataa cgtagtcgtc gaggacaacc ccggtccatc
     2401 cgttgtcctg ccaggcaaat tcatcgggat cgacgatctg ggacgggccg tggacgccca
     2461 ggggctggaa ccgggaagcg gggtccggtc gttctttgcc gccatccagc cggaagatat
     2521 accgcattcc cgacgacgcc ctttccagca tgccgctcca ataccccctg tcatcctttt
     2581 ccagagggac cgttacctct tcgggaaata cgatcttcac ctccacctcg ttcgagaagg
     2641 gggcccagac gaggaatgag cagatttcgt cttgaaggtg tgcgccgagt ttcatctccc
     2701 ctgccccgct gtccgcccgg ccttcgggat gatcctgatg cccgacgggt cgatgtggat
     2761 cccgctgtag agacggtcct tttcaacgtc gtcaccgagc accagtccct ccgtgaagac
     2821 attgaacttg tccgcgatca cccctttgag cctgcagccc ttgccgacta tggtgtgatc
     2881 catgatgatg ctgttctcca cgacggcgcc gtcctcgatc acgacgtcgc ggcggatgac
     2941 ggaattgacg atcctggcgt cttcgacgag cacaccctcg gaaatgatgc tgttttggat
     3001 ttctccccgg agaatcgtgg cggccggccc cttgtacccc gcgggctgaa tcggccagct
     3061 cctgttgtgc aggtcgagca gcggagtccg tcccagcatg tccatatgcg cctgatagaa
     3121 cgaagggatc gtccccacgt ctcgccagta gcccttctct tcgtaggatt tcgcgtccgg
     3181 gataatgttg gtcgcaaaat cataagcgta cacccttccc gggcccacca gcgaaggaat
     3241 gatgtgagcg ccgaagtcgt ggcgtccctc cttctgggcc ttgaccagtg agtcgacgag
     3301 gacgtcccgg ttgaagatgt aattccccat ggaaacgtat gacctgccgg ggtcgtccgg
     3361 catcggcgcg gggcgctccg gtttttcctg gaacccggtg atccttctct cccggtccgt
     3421 caggatgacc ccgaagtccg cggcctgtcc tatcgggacg ggtctggcgg ccagggtcac
     3481 ctcggcgccc ttttccaggt ggaaatcgat catctgccgg atgtccatgc gatagatgtg
     3541 gtcggccccg aaaacgatca ccagctcggg gtcctggtct tcgatgaggt tgacgttctg
     3601 gtacacggcg tccgccgtgc cctggaacca ctccggcccc atgcgcatct ggggcgggac
     3661 caccgccacg aaatggtctc ggatgacggg cgagagcacc cagttctggc gcacgtgctc
     3721 gatcagggac tgggacttgt attggaccag gagatagacg gagtagatat gggagttcac
     3781 caggttgctc agaacgaagt ccgcgatccg gaacctgcct ccgaagggca cggaaggctt
     3841 ggagcgcaga tcggtgagcg gcgcgaggcg ttctcccttg ccgcccgcca gcacgaatgc
     3901 cagaactttc ggatgggcca ttgccttcct ttcgggattc cgtgaatcag cggagctgcg
     3961 ctatttcgct gtaataattg acacgttcat gaaattttac tcgttgcgag ttgcgcgttg
     4021 cgggtttcga gtttaagccg caacccggaa cccggaacat cttctgcttt ccctcacgcc
     4081 ctcctgagaa gccgctcgat gctgcggatg gggatcacga tccagtcggg ccggttgtcg
     4141 agctcacggc ccagatcgtg cacggccctt tccatgagaa aacaccggag aagcgtctcg
     4201 acct
//
LOCUS       DASSLD010000209         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_90004,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000209 DASSLD010000000
VERSION     DASSLD010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90004"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /gene="rpsD"
                     /locus_tag="VFK23_03860"
     CDS             <1..225
                     /gene="rpsD"
                     /locus_tag="VFK23_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="primary rRNA binding protein; nucleates 30S
                     assembly; involved in translational accuracy with proteins
                     S5 and S12; interacts with protein S5; involved in
                     autogeneously regulating ribosomal proteins by binding to
                     pseudoknot structures in the polycistronic mRNA; interacts
                     with transcription complex and functions similar to
                     protein NusA in antitermination; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET7318255.1"
                     /translation="SYLLKAGDVVELREKSRGIVQIQQTLAAVEKRGFPSWLEIDKAQ
                     FRGKVLSLPARDESTLPTVKEQLVVELYSK"
     gene            338..1360
                     /locus_tag="VFK23_03865"
     CDS             338..1360
                     /locus_tag="VFK23_03865"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET7318256.1"
                     /translation="MLKKAKDFQMPKKLQYDQKKITDTYGKFTAEPLERGFGTTLGNS
                     LRRVLLSSLEGSAITSLKISGVQHEFSSIPGVIEDTTDLILNLKEVRLKLHTDKPKTL
                     LLKAKGPGEVTAKDIEADAEVEILNPDLHLATIDKNGKVELEMRARRGRGYVSAEKNK
                     EPGQSIGVIPVDSIFSPIRKVNFKVENARVGQETDYDKLILEVWTDGSIKPDDAVAHA
                     SKLLKDHLSIFIHLPEEEEELLVTDEEIKKAPSFNENLLRSVNELELSVRAANCLKNA
                     GIETIAEMVQKTESEMLKTKNFGRKSLNEIKEILAEMGLSLGMKVDDKIVGEARRLLA
                     EKPSEA"
     gene            1385..1807
                     /gene="rplQ"
                     /locus_tag="VFK23_03870"
     CDS             1385..1807
                     /gene="rplQ"
                     /locus_tag="VFK23_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET7318257.1"
                     /translation="MRHGCAGRQFGRNSGHRKALLRMLVTSLLKNEKIETTSAKAKEI
                     RPLAEQMITLAKRGDLHARRQALSFIQDDSVVGNLFSQIGPRFASRNGGYTRIVPTRN
                     RMGDAAPMAVIELVERQKKEAAEPKASGKAKAAKKASS"
BASE COUNT          612 a          583 c          638 g          500 t
ORIGIN      
        1 tcctatcttc tgaaggccgg tgacgtcgtg gaactccggg agaagagcag ggggattgta
       61 cagattcagc agaccttggc tgccgttgag aagcgtggat tcccctcgtg gctggagata
      121 gataaggccc agttcagggg aaaggttttg tccttgccgg ccagggacga gagcacgctg
      181 ccgacggtaa aagaacagct tgtcgtcgag ctctattcga agtaagtccc ttttatacat
      241 tacgaataat gccgagggca gcgtgagcgg ccgctttgac ggacgttccc tggtctttat
      301 cgtgtgaaaa gtttttttac tccgttctgg aggacccatg ctcaaaaaag ccaaagattt
      361 ccagatgccc aaaaagctcc aatatgatca gaagaagata accgatacgt acggcaagtt
      421 tacggccgag cctttggaac gcggttttgg gaccacgctg ggcaactcgc tgaggcgagt
      481 ccttctttcc tcgctggaag ggtctgcaat tacgtctctg aagatctcag gcgtgcagca
      541 tgagttttcg agcataccgg gtgtgatcga agatactacg gatctgatcc tgaatctcaa
      601 ggaggtcagg ctcaagctcc acaccgacaa accgaagacc ctgttactca aagccaaggg
      661 accgggcgag gtcacggcaa aagatatcga ggcggatgcg gaggtcgaga ttctgaaccc
      721 ggacctgcat cttgcgacaa tcgataagaa cggcaaagtg gaacttgaga tgcgcgcccg
      781 gcgcggcagg ggatacgtct cggcggagaa gaacaaggag cccggtcagt cgatcggagt
      841 catcccggtc gattcgatct tttccccgat ccgcaaggtc aatttcaagg tggagaatgc
      901 ccgtgtcggc caggagacgg actatgataa gctgatcctg gaggtctgga ccgacggcag
      961 cataaagcct gacgatgccg tcgcgcatgc gtcaaagctt ttgaaggacc acctctccat
     1021 ctttatccac ctccccgaag aggaagagga actcctggtt acggatgagg agatcaagaa
     1081 ggccccctcg ttcaatgaaa atcttctccg aagcgtcaat gagctcgagc tctccgttcg
     1141 cgcggccaac tgcctcaaga atgcgggcat agaaaccatc gcggagatgg tgcagaagac
     1201 cgagagtgag atgctcaaaa cgaaaaattt cggaagaaaa tcgctaaacg aaataaaaga
     1261 aatcctcgca gagatgggac tctcgctggg gatgaaggtt gacgataaaa ttgttggcga
     1321 agccagacgg cttcttgcag aaaaaccgag tgaagcgtaa tatcaacaga gtaaggatgc
     1381 caaaatgaga catggatgcg caggaaggca attcgggaga aactccgggc accgtaaggc
     1441 gctgctccgg atgctcgtta cctcgttgct gaagaacgaa aagatcgaga cgacatcggc
     1501 caaggcaaag gaaatccgcc ccttggcgga acaaatgatt accctcgcca agagggggga
     1561 tttgcacgcg cggcggcagg ccctcagctt tattcaggat gattccgtgg tcggtaatct
     1621 tttttcccag atcgggccgc ggttcgcgtc ccggaacggc ggctataccc gcattgtgcc
     1681 gacccgcaac cgcatgggcg atgcagcgcc catggcagtg atcgaactgg tcgagcggca
     1741 gaaaaaagag gctgcggaac ccaaagcttc aggaaaagcc aaggcggcga agaaggcatc
     1801 ctcctaggaa agaccctggc cggaagcggc cagggggcaa ccctccccaa aacttcgtcg
     1861 gatagggcag cggtaaattt ccgcctcaac gatattcatg tttaacccgg cataaccagg
     1921 atagtttcca tgccgggacc tccgttccca agcaaagacc gcttccacac gtatattcta
     1981 ttcgcgttta ccgataccct tcctctaata ctattgacct ctattggaga aagacatctt
     2041 atcctccatt caagaatgac tacctatcca atttgaacgg tttattctaa gcgtcctcgg
     2101 acttactcga cgaagagtga ttgcctaact agttgtatta ttgcactata tttaataaat
     2161 aatgcgcggc atgtcccttg cacatttatt attactacaa ttaatcttgc tagtaatcac
     2221 tgaaaatttg tgaggaggta ggatatgcgt aagtccttat tgccgttatt gtttgtgctg
     2281 gtggctgttc tagccttgac cgcatgtcac aggggcggaa gcagcgcacc gcc
//
LOCUS       DASSLD010000210         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1180758, whole genome shotgun sequence.
ACCESSION   DASSLD010000210 DASSLD010000000
VERSION     DASSLD010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180758"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1402
                     /gene="ftsH"
                     /locus_tag="VFK23_03875"
                     /pseudo
     CDS             1..1402
                     /gene="ftsH"
                     /locus_tag="VFK23_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946288.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
     gene            1412..>2020
                     /locus_tag="VFK23_03880"
     CDS             1412..>2020
                     /locus_tag="VFK23_03880"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET7318258.1"
                     /translation="MGPVLRISGRGTLFLTVSSLTSGQAAAIRNIIRAVDPPTSGAGP
                     GPAPSFSLSGSPEGITVSGSPDFVRRMARGISARSGLSSGLAGKIESLLDNYLRSDYK
                     IECRGKILDLGARTHIMGVLNVTPDSFSDGGRYARPDQALACAREMAAAGADIIDIGG
                     ESTRPGADPVPEEEELRRTVPLIERLAAELTVPISIDTYKSSV"
BASE COUNT          422 a          645 c          631 g          322 t
ORIGIN      
        1 ctacttcggc aaaagcaagg cgaagctcct gagcgaaagc tccaagaagg tcacctttaa
       61 tgaagtcgcc ggcatggatg aggccaaggt cgaggtccag gaaatcagcg agatcctgaa
      121 ggaccctcag aagttccaga agctcggcgg caagattccc aagggcgtgc ttctggtcgg
      181 ccctccggga acgggcaaga cactcctcgc gcgggcaatc gccggcgagg ctggcgtgcc
      241 gttcctctcg atcagcggat ccgacttcgt cgagatgttc gttggcgtgg gcgcttcgcg
      301 cgtccgcgac ctcttcgagt agggcaagaa gaacgcgccc tgcatcatct tcatcgacga
      361 gatcgatgcg gtagggcggc accgcggtgc gggcctgggc ggcggccatg acgaacgcga
      421 acagaccctg aaccagctcc tggtcgagat ggacggcttc gaatcgaacg agggtgtcat
      481 cctcgtggcg gccacgaacc gccccgacgt gctcgacccc gcgctcctca ggccgggcag
      541 gttcgaccgc caggtagtga tcccccgtcc cgatctgaat ggcagggtcg gcgttttgaa
      601 ggtccatacc cagaagatca agctcgcgcc tcacgtagac cttgaatcca tcgcgcgcgg
      661 caccccgggc ctctcggggg cggagttggc gaacctcgtg aacgaggccg cgctcctggc
      721 aggcaggatg ggcaagacgc aggtggaaaa cgacgacttt gaccgtgcca aggacaagat
      781 cctcatgggc ctcgaacgca agagcatgat aatcagcgac gaggagaagc ggaacacggc
      841 aacccatgaa gccggtcaca cgatggtcgc aaagctcatt ccgggcaccg acccggtgca
      901 caaggtctct atcatcccgc gcggtatggc cctgggcgtc acgctccagc ttcccacggc
      961 cgaccgctac tcctacgacc gcgagcacct cttgaacaac atcgcgatcc tcctgggagg
     1021 cagggcggcg gaagagatcg cgctccagca tatgaccacc ggcgcaggca acgacatcga
     1081 acgcgctacc gcgctcgcgc ggaaaatggt gactgaatgg ggcatgagcg acaaactggg
     1141 ccccctcgcc tacggcaaga aggaagagca gatattcctg ggacgcgaga tcgcccagca
     1201 ccgcgactac agcgagcaca cggccatcga gatagacgag gaagtgaagc gtattgtgtc
     1261 cgataactac gagcgggcca agaagctgat cacggaccgc cgggaaacgc tcgacaggct
     1321 gaccgcggcc ctgcttgaaa aggaaaccct cgacggcccc gagatcgagt tgatcgtcca
     1381 cggccagccg cagacggtgt aggaggcggg catggggcct gttctccgga tctccggccg
     1441 tggaactctc ttcctgaccg tgagcagctt gacatccgga caggcggccg ccatccgcaa
     1501 tatcatcagg gcagtagacc caccaaccag cggggcaggt cccggacctg ccccgtcttt
     1561 ttccctttcc ggctcgccgg agggcatcac ggtctccggt tccccggact tcgtgcggcg
     1621 catggcgcgc ggtatctcgg ccagaagcgg cctttcctca gggctcgcgg gaaaaatcga
     1681 atctctgctt gacaactatt tgcgctcgga ctataagatt gagtgcagag ggaagattct
     1741 cgacctcggc gcccgaacgc atatcatggg ggtcctgaat gtgacccccg attccttttc
     1801 cgacggcgga cggtacgccc gcccggacca ggccctcgcc tgtgcgcgag agatggcggc
     1861 tgccggcgcg gacatcatcg atatcggcgg tgagtccacc cgtccgggag ccgatccggt
     1921 gccggaggaa gaagagctgc ggaggaccgt ccccctcatc gaacggctcg ccgccgagct
     1981 gaccgtcccc atttccatcg acacctacaa atcatcggtc
//
LOCUS       DASSLD010000211         4352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_427252,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000211 DASSLD010000000
VERSION     DASSLD010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427252"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /gene="gpmI"
                     /locus_tag="VFK23_03885"
     CDS             complement(<1..977)
                     /gene="gpmI"
                     /locus_tag="VFK23_03885"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HET7318259.1"
                     /translation="MAVKRVLLIVLDGWGVNMNKEGNAIAAAKAPVYERLIADYPHAE
                     LEASGEAVGLPDGQMGNSEVGHLNLGAGRIVYQDSTRISKSIRDGAFSRNPALLAALE
                     KVKKNRSSLHLMGLLSDGGVHSRLDHIFAMFDLVKAQGITNVFFHAFLDGRDTPPSSA
                     IRYVRQLEDHLSRIGIGRIASLSGRYYAMDRDKRWERVQKAYEAVVLGEGIRKYSAVE
                     AIEQSYEHNRTDEFVLPTVILDPSTNKPLSTMRTGDAVVFCNFRSDRAREITRALTDP
                     EFQGFSRGAVPKLATFVCLTTYDDTFDLPVAFSPVKLTNILGEVLSNRGL"
     gene            complement(1091..2398)
                     /locus_tag="VFK23_03890"
     CDS             complement(1091..2398)
                     /locus_tag="VFK23_03890"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7318260.1"
                     /translation="MRFISTLLLIGIVIVVILLREFNSDLITIYLLPSKSFEISKSTV
                     LILSLAIGAGLVFLVYLTRDLRRFLRGLSVQREQKKRMKVQELYTKGLNAMLAKRNAE
                     AANYFQKVLAVDPNHVDTLLRLGICKLRDKMPQEAILVHQKALGFAPNNQEVLFALAA
                     DYEETKRFDDALEMYKRILNRDSSNLTAIIRMRDLYQRLSQWEELYETQSKLVANPLT
                     PSEMEIEHRKLVGLKYEFGRSLLEAGDLERAKKIFRGVIKLDKDFIPAYLGLGEVYFE
                     ESKASEAAELWEKAYKMTSSTLLLHRLEDLYLRQGEPGKAIELYMKAVNWNPQDVRLK
                     FFLGKLYYRLEMVDEAFDILSTVDWGDKEFPDVHKLLGNIYLRRGSLGLAASEFKKAL
                     GFKKQIIIPYTCSNCDLRTKDWSGRCPNCGKWNTFGVNLEKTC"
     gene            complement(2483..2953)
                     /locus_tag="VFK23_03895"
     CDS             complement(2483..2953)
                     /locus_tag="VFK23_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET7318261.1"
                     /translation="MRVAVVCVGKTRERFIQEGIVKYLRYLKPYTDVEIKELKEEKIQ
                     DLRDAPRIRKREAEKIFKAASPGTYLAALDERGSEYTSHEFAHFLNGLMESGKKEMTF
                     VIGGAMGLDESVAEKANKVIALSRLTLTHEMARLVFLEQLYRAFTIIKGKTYHY"
     gene            complement(2950..3315)
                     /gene="rsfS"
                     /locus_tag="VFK23_03900"
     CDS             complement(2950..3315)
                     /gene="rsfS"
                     /locus_tag="VFK23_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET7318262.1"
                     /translation="MEAAQLCAEAADNKKAFDILILDLRGLTYITDYFVICSGNNTTQ
                     VSAISEEVGHILAKAGEHPSHVEGGAESNWVLMDYGDVVVHIFEEQTRMFYSLEKLWS
                     DAPRIPVAIRPRALQGASS"
     gene            complement(3320..3979)
                     /gene="nadD"
                     /locus_tag="VFK23_03905"
     CDS             complement(3320..3979)
                     /gene="nadD"
                     /locus_tag="VFK23_03905"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET7318263.1"
                     /translation="MTSRIGILGGTFNPIHYGHLAAAEEVRDRLKLDRILFIPSSIPP
                     HKHEEDVPPAEHRMEMVRLALAGNPCFEASDLEIRRGGRSYTIDTVEMLLQSHPGAAL
                     FFITGVDSFLEIQTWNRWDRLVSLCSFVVISRPGYGFRDLAKIDFMKSAVPELSGLDR
                     GARSQWVVKSGAITVYLETVPLYDISSTDIRKRMREGRSIKYHLPEHVETYIINHKLY
                     A"
     gene            complement(4085..>4352)
                     /gene="proA"
                     /locus_tag="VFK23_03910"
     CDS             complement(4085..>4352)
                     /gene="proA"
                     /locus_tag="VFK23_03910"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222928.1"
                     /note="Catalyzes the phosphorylation of L-glutamate during
                     the proline biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HET7318264.1"
                     /translation="MAHIAAHGSQHSEAIVTNDYRNAMRFHREVDASAVFVNASTRLN
                     DGFEFGLGAEIGISTTRIHARGPMGREELTSTKFIVFGNGQIRR"
BASE COUNT          861 a         1250 c         1226 g         1015 t
ORIGIN      
        1 agcccccggt tgctcagcac ctcgccgagt atgttcgtga gtttgaccgg actgaacgcg
       61 acggggagat cgaacgtatc gtcataggtg gtgagacaaa cgaacgtggc aagtttcggg
      121 accgctcccc tgctgaaacc ctgaaattca gggtcggtaa gcgctcgcgt gatctcgcgg
      181 gctcgatccg agcggaagtt gcagaagacg accgcatcgc cggttcgcat ggtcgaaaga
      241 ggcttgttcg tgctcgggtc gaggatcacc gtcggcagca caaattcgtc ggttcggttg
      301 tgctcgtagc tctgttcgat cgcctcgacc gcggagtatt tccggatgcc ctcccccaga
      361 accacggcct cataggcctt ctgaacgcgc tcccatcttt tgtcccggtc catggcatag
      421 taccgtcccg acaggctcgc tatgcggccg atgcctatcc tgctcaaatg gtcctcgagc
      481 tgcctgacat accggatggc gctcgagggc ggcgtgtctc tcccgtcgag aaaggcgtgg
      541 aagaagacgt tcgtgattcc ctgcgccttc acgagatcga acatcgcgaa gatgtggtca
      601 agacggctgt gcacgccgcc gtcggagagg agccccatga gatggagcga tgaccggttc
      661 tttttcactt tttccagcgc tgcaagcagt gccgggttgc gggaaaaagc accgtctctg
      721 atggacttgc tgatcctcgt cgaatcctgg tagacgatcc tccccgcgcc gaggttcagg
      781 tgtccgacct cggaattgcc catctgtccg tcgggaagac ccaccgcctc gcccgatgcc
      841 tccagttccg catggggata gtccgcgatg aggcgctcat agacaggcgc cttcgctgct
      901 gcaatggcat tcccctcctt gttcatgttg acgccccatc cgtcgaggac gatcagcagc
      961 acccgtttga cagccatgaa gactccttaa aataaaggag tcagaagcca gcagtcggga
     1021 gtcagaatgg accgaaacga cttttccgtc tcctgacgcc tggctcctga ctcctgactc
     1081 ctcctgtttt ttagcaggtc ttttccagat tgaccccgaa ggtgttccac ttgccgcagt
     1141 tcggacaccg tccggaccag tctttggtcc tcaggtcgca gtttgagcaa gtgtagggga
     1201 tgatgatctg cttcttgaaa cccaatgcct ttttgaactc cgacgcggcc agcccgaggg
     1261 aaccccttct caggtagatg ttccccagga gtttgtgcac atcgggaaac tccttgtcgc
     1321 cccagtccac ggtggaaagg atatcgaagg cctcatccac catttcgaga cgatagtata
     1381 gcttgcccag gaaaaacttg agtcttacgt cctgcgggtt ccagttcacg gccttcatgt
     1441 aaagttcgat ggccttgccg ggttctccct gcctcaggta gaggtcttcc agacggtgga
     1501 gcagcagggt cgacgaagtc atcttgtagg ccttctccca gagttcggcc gcctcagagg
     1561 ccttgctttc ctcgaaatag acctcgccga gtcccaggta tgcggggatg aaatccttgt
     1621 ccagttttat caccccgcgg aatatcttct ttgcccgttc caggtccccg gcttccagaa
     1681 gggacctgcc gaattcatac ttgagcccga cgagtttgcg gtgctcgatc tccatttccg
     1741 aaggcgtcag gggattggca acaagcttgc tctgggtttc gtacagttcc tcccattgac
     1801 tcagccgctg gtagaggtcg cgcatcctga tgattgcggt caggttggac gagtcccggt
     1861 tcaggatcct cttgtacatc tccagggcgt catcgaaccg tttcgtctct tcgtaatcgg
     1921 cggctagtgc gaacaacact tcctggttgt tcggggcaaa accgagcgcc ttttgatgga
     1981 ccaggatcgc ctcctggggc atcttgtcgc ggagtttgca gatgcccagg cgcagcaggg
     2041 tgtccacgtg gttcggatcg acggcaagga ccttctggaa atagttggcg gcttcggcat
     2101 tgcgtttcgc gagcatggca ttcaggccct tcgtgtaaag ctcctggact ttcatgcgct
     2161 ttttctgttc gcgctgaacg ctcaaccccc gcagaaaacg cctcaggtcc cgcgtgagat
     2221 agaccagaaa tacgaggccg gccccgatgg ccagagagag aatcaggacg gtggacttgg
     2281 agatctcgaa ggacttcgac gggagcaggt agatcgttat caggtcggaa ttgaactcgc
     2341 gcagcagaat cacgacgata acgatgccta tcaacaggag ggtgctgatg aatctcatcc
     2401 gcggtccttt ctcacatgat aatgacgggt tctgttgcct gagactcttg gataattccg
     2461 aattccgcat tcggcattgg ggctaataat ggtacgtctt gcccttgatg atcgtaaatg
     2521 cccggtagag ctgttcgaga aaaacaagcc tcgccatctc atgggtgagg gtaagccggg
     2581 acagtgcgat caccttgttt gctttttcgg caacgctctc atccaggccc atggcccccc
     2641 cgatgacgaa cgtcatttcc ttctttccgg actccatcag cccgttcaga aaatgtgcga
     2701 actcgtgaga ggtatattcg gagccccgct cgtcgagcgc tgcgaggtag gtccccggag
     2761 acgccgcctt gaatattttt tccgcctcgc gctttctgat gcggggcgcg tccctgagat
     2821 cctgtatctt ttcttctttg agttctttga tttctacgtc ggtatagggt ttgagataac
     2881 ggaggtactt gacgatcccc tcctggatga accgctctct ggtttttccc acgcagacga
     2941 ctgccaccct catgaagagg cgccctggag tgcccgcggc cttatggcga cgggtattcg
     3001 cggagcgtcg ctccagagtt tttcaaggct gtagaacatc cttgtctgct cttcaaagat
     3061 gtgcacgacg acatcgccgt agtccatgag cacccagttt gactcggccc ctccctctac
     3121 gtgggaagga tgttctcccg ccttcgcaag gatatgcccg acctcctcag aaatggcgga
     3181 cacctgcgtc gtgttgtttc ccgagcagat cacgaaataa tcggtgatgt aagtgagccc
     3241 gcgaaggtcc aggatgagaa tgtcgaacgc ttttttgttg tcggctgcct cggcgcaaag
     3301 ctgcgccgct tccaaagaat tacgcataga gcttatgatt tattatatag gtttcaacgt
     3361 gttcaggcaa gtgatatttg atgctcctgc cctccctcat cctcttgcgg atgtcggtcg
     3421 aggaaatatc gtaaaggggc accgtttcca gatagacggt gatcgccccc gactttacga
     3481 cccactggga ccgggcgccg cggtccagac cggaaagttc ggggacggcg gacttcatga
     3541 aatcgatctt cgcgaggtcc ctgaagccgt aacccggccg tgagatcacg acgaagctgc
     3601 acagggagac gagcctgtcc cagcggttcc aggtctgtat ctcgaggaac gaatccacac
     3661 cggtgatgaa aaacaaggca gcaccgggat gggattgaag cagcatttcg accgtatcga
     3721 tggtatacga cctgccccct cgtctgattt ccaggtccga cgcttcgaag caggggtttc
     3781 ccgcaagggc caagcggacc atttccatcc ggtgttcggc cggcggcacg tcctcttcgt
     3841 gcttgtgggg cggtatggaa gaagggatga acagtatccg gtcaagcttc agacggtccc
     3901 ggacctcttc cgcggcggca agatggccgt aatggatggg gttgaacgtc ccgccgagga
     3961 tgccgattct tgatgtcaag tgaccccctt gtcaaggccg acggacgatg ggcgaccgtt
     4021 cgcttcgctc acttggacga aggacgaaaa agactttgcg tccctcgtcc cctcgtccct
     4081 cgctctatct cctgatctgt ccgtttccaa acacgatgaa tttggtcgag gtgagttctt
     4141 cccgccccat agggccgcgt gcatggatcc tcgtcgtgga aattccaatc tcggcgccga
     4201 ggccaaactc gaatccgtcg ttcagccgcg tcgaggcgtt cacgaaaaca gcagacgcgt
     4261 ccacctcacg atggaaccgc attgcgttcc ggtagtcgtt ggttacgatt gcctcggagt
     4321 gctgcgagcc gtgggcagcg atgtgcgcca tc
//
LOCUS       DASSLD010000212         2845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_942649,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000212 DASSLD010000000
VERSION     DASSLD010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942649"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..997)
                     /locus_tag="VFK23_03915"
     CDS             complement(215..997)
                     /locus_tag="VFK23_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase accessory protein
                     CooC"
                     /protein_id="HET7318265.1"
                     /translation="MKIAITGKGGVGKTTLAGLLARLYAAEGKKVLAVDADPDANLAS
                     ALGISPERAARALPLAEQADLIEERTGSRPGKPGGMFSLNPRVDDIPDTYAISHEGVR
                     LLVVGKSKEAGSGCYCPENVLLRRLISHLILKLDEVVILDMEAGIEHLTRGTAGGVNA
                     FIVVVEPGQRSMQTARQIESLAHDLGIKDVFVVGNKIRQESEKDFITAHLKGMTFLGF
                     MSYTADAVAADLDGKSPYDASPATRDEAKKIKAALDSLIKLP"
     gene            1246..2301
                     /gene="rlmN"
                     /locus_tag="VFK23_03920"
     CDS             1246..2301
                     /gene="rlmN"
                     /locus_tag="VFK23_03920"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET7318266.1"
                     /translation="MSNSRLNIKSLSREELRSMVSLLGLERYRADQVLKWIYSGYASS
                     FDDMTNIAKKDRATLSETFLIPRPAIAKTEASSDGTRKFLFELEDGHTVESVLIPDED
                     RQTLCISSQIGCRQACRFCLTGSGGFVRDLKAFEITDQVLEVSRLVKREGTRGVTNIV
                     LMGMGEPLANFDEVLKALKIITSEAGLGFSPRRVTVSTDGLVPEIGKLGKSGIKVNLA
                     VSLNATTDEVRDRIMPVNRRYPIKELLDSCRRFPLEPRRRITFEYVLLKGVNDSVEDA
                     LRLAKLLRGIKCKVNLIPFNPFPGSEFKRPDEKAVRRFQKVLLDHHYTAPVRESRGRD
                     ISAACGQLREKEAAASV"
BASE COUNT          659 a          768 c          764 g          654 t
ORIGIN      
        1 gctcaatttc acggctcttg cccctgcgca catcgcgcgg caggctgtcc ctcgtttccg
       61 cagcttgttt tctcagagac cttttcaaat gggggcttgc gactgataat tttttgaaat
      121 gactctataa tcgttactta tagtaacata gaacaaaata gtcgtccagt cttttttttt
      181 catgggtctc atggtcctta tttcgggcct acgtctacgg tagtttgatt aaactgtcga
      241 gggcggcctt gatttttttt gcttcgtctc ttgtggcagg cgaggcgtcg tacggagatt
      301 tgccgtcgag gtcggccgcg accgcgtcgg cggtgtaact catgaagccg agaaaggtca
      361 tccccttaag atgggcggtg ataaaatcct tctccgattc ctgccggatc ttgtttccga
      421 ccacaaaaac atccttgatg ccgaggtcat gcgcgagcga ctctatctga cgcgcggtct
      481 gcatgcttcg ctggccgggc tccaccacga cgatgaatgc attgacgccg cccgcggtgc
      541 cgcgggtcag gtgttcgatg ccggcctcca tgtcgaggat gaccacttcg tccagcttga
      601 gaatcaaatg actgatgagc ctccgcagga gcacgttctc ggggcagtag catcccgaac
      661 ccgcctcttt ggacttcccc acgaccaaaa gcctcacacc ctcatggctg atggcgtagg
      721 tgtcgggaat atcatccacc cgcgggttga ggctgaacat gccgccgggc ttgccgggtc
      781 gggagccggt gcgctcctcg atcaggtccg cctgctccgc aagcggcagg gcgcgggccg
      841 ccctctcggg tgaaataccc agggccgagg ccaggttcgc gtcagggtcg gcgtccaccg
      901 ccagcacctt cttgccctcc gccgcgtaca gccgcgccaa aaggcctgcc agtgtggtct
      961 tgccgacgcc gcccttgccg gtaatcgcga ttttcaatca aatcacctcg aaaagtagcg
     1021 aataaaataa catctatcga gcgggagtgt caagatgtat ttggctgccc tgccgtcggg
     1081 cgggtgtgtg gcaacgaggg aatgcgagat cattacgcga gagacctaag cctgcggagg
     1141 ggctcagagc cggccctagt tgatcaacgg taatctttac acggtaatct tgccaacttg
     1201 cttttccggg ataaaggtgt tacagtccgt caaggcgatg aatgcatgtc gaattcccgt
     1261 ttgaatatca agtcattgtc ccgcgaggaa ctccgctcca tggtatctct gctcgggctc
     1321 gaacgttacc gggctgacca ggtcctcaaa tggatatatt caggttatgc atcctcattt
     1381 gatgacatga cgaacatcgc aaaaaaggac cgtgcgaccc tgtcggagac ctttctcata
     1441 ccgagaccgg cgatagccaa aacggaggcc tcttccgacg gcacgagaaa attcctcttc
     1501 gagctcgagg acgggcacac ggtcgaaagc gtcttgatcc ccgacgagga ccgccagacg
     1561 ctctgcatat cttcgcagat cgggtgccgt caggcatgcc gcttctgcct gaccgggagt
     1621 ggggggttcg tgagagacct gaaggccttc gagatcaccg accaggtatt ggaggtttcc
     1681 cgcctcgtaa agcgggaagg aacgcggggc gtcaccaaca tcgtcctgat gggaatggga
     1741 gagccgcttg cgaacttcga cgaagtcttg aaagccctga agatcattac atcggaggca
     1801 gggctcgggt tttcaccgag acgcgtgacg gtttctaccg acggtctggt tccggaaata
     1861 gggaagctgg gaaaatccgg gataaaggtc aacctggccg tatccctgaa cgccacgact
     1921 gacgaagtac gggacaggat catgccggtc aaccgccgct atccgatcaa agagctcctg
     1981 gattcctgca gacgtttccc tcttgagccg aggcgcagga tcaccttcga atacgtgctg
     2041 ctaaaaggag tgaatgattc cgtcgaggac gccctgcgcc ttgcgaaact cctccgggga
     2101 atcaagtgca aggtgaactt gatcccgttc aatccttttc ccggaagcga gttcaagagg
     2161 cctgatgaga aggcggtgcg ccgtttccag aaagtcctcc ttgatcacca ttacactgca
     2221 ccggtccgtg agtcgcgcgg cagggatata tccgcggcgt gcgggcaatt gcgggagaaa
     2281 gaagcagcag cttcagtata atttttttta tcactctgtc gaactaagcc ataaagctca
     2341 gcagccatgc gagctgacaa cggtcgtgat gaatatcagg accaaagact tacctcaccc
     2401 atcaaattcc ctccaataaa aaatcctaag tactaccctg cagaactgac attctcaacc
     2461 ttcccgccta ttcgatacag tcgattacca ttgcgaccaa agattgatca taaccactca
     2521 aaagtatgct cgaaaacaaa atccaaagta ctatctctcc gatgcataat gttgccgtgt
     2581 tgcgcaactt gtgttatctt aaaagagagg gagatttcaa acacaaactt tcggcatggt
     2641 aatgtctctg ttatgatagc ggcaatattg aaaagagtca ttcgcgaaca ttcattctac
     2701 acagagaaaa ggttactatg cggaaaggag gtataaggct ccatgaagac gaatcgtaaa
     2761 agtctcatta taatagcgct ggtaatggcc gtggcgtttc ttccggtttc agtaggacgg
     2821 gcatttgctg ctgctatcga tgtac
//
LOCUS       DASSLD010000213         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_691219,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000213 DASSLD010000000
VERSION     DASSLD010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691219"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VFK23_03925"
     CDS             <1..1246
                     /locus_tag="VFK23_03925"
                     /inference="COORDINATES: protein motif:HMM:NF025016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HET7318267.1"
                     /translation="INYVVFGGRLLRNVHKWAGEGMIIFVMLHMARVFYTGSYKQGRE
                     FNWVVGIALLVCTFGINLSGYMLPWDQLAYWALVIVSNIIQSPKEITDSLGITQYFDI
                     GGLSKELFLGGLHAGKESLTRVYLLHIMLLPMLLFIFLGIHFWRIRKDGGLTKPEHFE
                     PSKGDREYLSPPEKKGGIFTTNKTYGLMELARGRTPAVGKDIEHEVLSWPNLLIAEAA
                     LFMLCLAAVVVYAFFIDAPLKELANPMIPENPAKAPWYFLGLQELLSYSAFMGGVGLP
                     GLALLGLALLPYLDREQDHVGVWFSNQQGKKTALEALVVATVVLIGILAFVINFGWFR
                     AWWPNVPQLVITLINPGNIWVAFMIAWTVFVIKRTGSTRMGAIALFTMFLVGFVILTY
                     VGTELRGPNWAFYWSTSQWPPH"
     gene            1322..>2327
                     /locus_tag="VFK23_03930"
     CDS             1322..>2327
                     /locus_tag="VFK23_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7318268.1"
                     /translation="MRNRFYFWVLFLSGAVLLIFTVIAGYREFSPEWAGYQSEYKKEL
                     IKTVKDEAAKSKARSLPIEIQQLYLGDLNRIDRCASCHIGYDNPLMAKAKAPLKEHSG
                     DYLKVHSPEKFGCTVCHYGQGRATNRKEAHGLGHDTHWDFPIIPAKYVQSACAQCHDS
                     AMLQAKGWDQPARGEKLVNEKGCRGCHKLNNVGGVLGKALEGVGSKPIAYFPMGAVQG
                     EKTTYSWFKQHFVDPRNLVPESEMKVIATDEEADLLTTYVLSLRAGEMPKQYRRFAAT
                     DGSKQPADGETLYKQYCIACHGEGKDSIFDETFSRTVPAIKNPAMLRALDNKHLKKIV
                     S"
BASE COUNT          523 a          641 c          670 g          493 t
ORIGIN      
        1 catcaattac gtcgtgttcg gcggaagact gctccgaaac gtgcacaagt gggcgggaga
       61 aggcatgatc atctttgtca tgctccacat ggcgcgggtc ttctacaccg ggtcctacaa
      121 acaggggcgg gagttcaact gggtggtggg aatagcgctc ctcgtctgca ccttcgggat
      181 caacctgtcg ggatatatgc tcccctggga ccaactcgcc tactgggcgc tcgtcatcgt
      241 ctcgaacatc atccaatcgc ctaaggaaat caccgactcc ctgggcatca cgcagtattt
      301 cgacatcggc ggcttgtcca aagagctctt cctgggcgga ctgcacgcgg gcaaggagtc
      361 ccttacccga gtctacctgc tccacatcat gctccttccg atgttattat tcatctttct
      421 cggcattcat ttctggagga tcagaaagga cggaggcttg acgaaaccgg aacactttga
      481 gccgtctaaa ggcgacaggg aatatctttc acctcccgaa aagaagggcg gcatattcac
      541 gacgaacaag acctacgggc ttatggagct ggctcgcggc aggacgccgg cggtcggcaa
      601 ggatatagag catgaggtcc tgagctggcc caatctcctt atcgccgagg cggcgctctt
      661 catgctctgt ttagccgcgg tggtcgtcta tgcattcttc atcgacgcgc cgctcaagga
      721 actcgccaat cccatgatac cggaaaatcc ggccaaggcg ccctggtatt tcctcggatt
      781 gcaggaactc ctctcctatt ctgcgttcat gggcggcgtg ggccttcccg gactggcgct
      841 tctgggactg gcgctgttac cctacctcga ccgggaacaa gaccatgtgg gtgtctggtt
      901 ctccaatcag caaggaaaga aaaccgcgct cgaggctctc gtcgttgcaa cagtagtgct
      961 catcggaatt ctggcttttg taatcaattt cggctggttc agggcctggt ggccgaatgt
     1021 gccgcagctt gttattaccc tgattaatcc cggtaatatc tgggtcgctt tcatgatcgc
     1081 ctggacggtc ttcgtgatca agaggaccgg ttcgacgcgg atgggcgcca ttgccctctt
     1141 cacgatgttt ctcgtgggtt ttgtgatcct tacctacgtg gggacggaac tccgggggcc
     1201 gaactgggcc ttctactggt ccacgagcca gtggcctccc cattgatgcg ggggcttagc
     1261 aaggcagcag cagagaattt cgtcgtcatt agcagatata cacattgaga aggggagaac
     1321 tatgcgcaac aggttttatt tctgggtatt gttcttgtcg ggagcggtcc tgctcatctt
     1381 cacggttatt gcaggttacc gtgaattctc tcccgaatgg gcaggttatc agtcagagta
     1441 caaaaaagaa ctcataaaga ccgtgaagga cgaggcggcg aaaagcaagg cccgttccct
     1501 gccaatcgag atccagcagc tatacctcgg cgacctgaac cggatcgatc gatgcgcgag
     1561 ctgccatatc gggtatgaca acccgctcat ggcgaaagca aaagcgccgc tcaaggagca
     1621 cagcggcgac tacctcaagg tgcattctcc cgagaagttc gggtgcacgg tgtgccatta
     1681 cggccagggc cgagcgacca acaggaagga ggcgcacggc ctcgggcatg acactcattg
     1741 ggactttccc atcatcccgg caaaatacgt ccagagcgct tgcgcccagt gccacgattc
     1801 cgcaatgctt caagcgaagg gatgggacca gcccgccagg ggcgaaaaac tggtcaatga
     1861 gaagggatgc aggggttgtc ataagttgaa caacgtaggc ggcgtcctcg ggaaagccct
     1921 cgaaggcgtg ggctcaaagc ccattgcgta cttccccatg ggagcagtcc agggagaaaa
     1981 aacgacttac tcgtggttca agcagcattt tgtcgatccg cgcaacctcg ttcccgaaag
     2041 cgagatgaaa gtcatcgcta cggacgagga ggcggacctg ttgacaacct atgtgctgtc
     2101 gctcagggcc ggagaaatgc cgaagcagta ccggcgtttt gcggcaacgg acggctctaa
     2161 gcagccggct gacggcgaga ccctgtataa acagtactgc atagcctgtc acggcgaggg
     2221 caaagacagc atttttgacg agacattcag ccgcacggtg cccgcgatca agaacccggc
     2281 catgctgagg gcgctggata acaagcacct gaagaagatc gtctcgg
//
LOCUS       DASSLD010000214         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1604474, whole genome shotgun sequence.
ACCESSION   DASSLD010000214 DASSLD010000000
VERSION     DASSLD010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604474"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VFK23_03935"
     CDS             <1..568
                     /locus_tag="VFK23_03935"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7318269.1"
                     /translation="DRYTSYKKRCEQILSEGAEGVEYVVIRPGVVYGPGSRHLGNLVK
                     TVKRLGPLGFPWIGRGRAVAPFIHVKDLARAIYLAGIEPAASGQVINLTDGADNTWSD
                     FFHAIAGALGVSLRVLPVPPLFLSAPALFFDIAGLLWGSRLDLNTYLRYVTTDLLFDN
                     GKAQRLLRWKPEYTLLRGVEEMVREYGM"
     gene            653..1204
                     /locus_tag="VFK23_03940"
     CDS             653..1204
                     /locus_tag="VFK23_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013295388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7318270.1"
                     /translation="MDTRFRGYDSGGGAMNALETIFSRRSVRLYTPKPVSHESIHDIL
                     KAAMSAPSAGNERPWHFIVLTDRALLDEIPKFHPYSAMLKYAQAAILVCGDLTLERHA
                     GYWVLDCAAATENILLAAHAKGLGAVWCGVYPTEDRVQNLRRLLKLAEHVVPFSLVPL
                     GFPAEVKETRDRYDGSRVHENKW"
     gene            1398..>2542
                     /locus_tag="VFK23_03945"
     CDS             1398..>2542
                     /locus_tag="VFK23_03945"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HET7318271.1"
                     /translation="MKMQKLRQLLRPSGFKVGLGLTVLSLVIYAMGFPFLNAIELKAY
                     DLHFLSRGKTQPGSEVVIATIDEKSIDRIGRWPWPRTKIAELIDKLGKYGARVVAFDV
                     FFSEPDQSSGAATFRDLQARATDRAAKAALEAAARAADNDARMTAALRRNQNVTLGYF
                     FYLKKEDSKFRKEKTGSEAEFILPSSFSSIRYLEQEKQPPALIKAFDVVQNIPQLAAA
                     TSSFGYLNIIPDEDGTVRWVPLAVQYGDEIYPHISLEAVRRYLGSPPLILNAASYGVN
                     SIQIGRLVIPTDEWGRLLVNYRGPQKTFPHYSIIDVLDGTVPKEALKDKIVLVGATAI
                     GIYDMRVTPFSGTFPGVEIHANTIDNILRTDAIKRPDLITLFDVTAIL"
BASE COUNT          527 a          766 c          740 g          509 t
ORIGIN      
        1 agaccggtac acatcgtaca agaaacggtg cgaacagatc ctttccgaag gggcagaggg
       61 cgtggagtac gtcgtgatca ggcccggtgt ggtctacggc ccgggctccc ggcacctcgg
      121 gaacctcgta aagacggtca aaaggctcgg ccccctcggg ttcccctgga tcggcagggg
      181 ccgtgccgtt gcgcccttca tccacgtcaa ggacctggcg cgcgccatct atcttgcggg
      241 catcgagcca gccgcctccg gtcaggtaat caacctgaca gacggagcgg acaacacctg
      301 gtcggacttc tttcatgcca tagcaggcgc actcggcgta tcgctgaggg tccttcctgt
      361 tccgccgctt tttctgagcg cgccggccct gttcttcgac atcgccggtc tgttgtgggg
      421 ctccaggctt gacctcaaca cctatctccg gtatgtaacg accgacctcc tgttcgacaa
      481 cgggaaggcg cagcggcttt tgcgctggaa gccggagtac accctgctgc ggggagtcga
      541 agagatggtg cgggagtacg gaatgtaacg gcactaatac tacagcaaaa tccttttttg
      601 tcattcctgc aaaagcagga atccatttta aaatttctcc ggggtaaggc caatggatac
      661 ccgctttcgc gggtatgaca gcgggggtgg agccatgaat gcccttgaaa ccatcttttc
      721 acgccgcagc gtgcggcttt acacgccgaa acccgtttct cacgagtcga tccacgatat
      781 tctgaaggct gcgatgagcg cgccttcagc aggcaacgag cggccctggc atttcatcgt
      841 gctcacggac cgcgccctcc tcgacgagat cccgaaattc catccttatt cggcgatgct
      901 gaaatacgcg caggccgcca tcctggtctg cggagacctg acactcgagc ggcacgccgg
      961 ctactgggtc ctcgactgcg cggctgcgac cgagaatatt cttctggccg cccacgcaaa
     1021 aggtctcggc gcggtgtggt gcggagttta tccgacggag gaccgggtcc agaacctccg
     1081 gagactgctc aagctggcgg agcacgtcgt gcctttttcg ctggtccccc tcggctttcc
     1141 ggccgaagtg aaggaaacaa gggaccgcta tgacggttcg cgggtccatg agaacaagtg
     1201 gtaatgccct gtcgattggt atggtatttt gccctgtctt ttcccgatca gatgcctaaa
     1261 aaatcccgcc gcggccctgt actttttttt gcatatgagc taaagtgttt gccaatataa
     1321 ttacgaaatc gtattttcac ctcggaaacg cgggcggcaa tgacggtgaa gaacagcgat
     1381 ccagggagtc aagaatcatg aaaatgcaaa agctgaggca actcctcagg ccttccggct
     1441 tcaaggtcgg gctgggcctc acggtcctgt ccctggtcat ctacgcgatg gggttcccgt
     1501 tcctgaacgc gatcgagctg aaggcctacg acctccattt cctgtcgcgg ggcaaaacgc
     1561 agcccggcag tgaggtcgtg atcgcgacca tcgacgagaa gagcatcgat cgcatcggcc
     1621 ggtggccctg gccgcggacg aagatcgcgg agctgatcga caagctgggc aaatacgggg
     1681 caagggtcgt ggcttttgat gtcttctttt cagagccgga ccagagttcg ggggccgcga
     1741 cattccggga tcttcaggca agagcgacgg accgcgctgc gaaagcggcg ctggaagcgg
     1801 cggcccgggc agcggacaac gacgcgcgga tgaccgcggc ccttcggaga aaccaaaacg
     1861 tcacgctggg ctacttcttc tacctgaaga aagaagacag taaattccgg aaggagaaaa
     1921 cggggagcga ggccgagttc atcctcccgt caagcttttc gtccattcgc tatctggagc
     1981 aggaaaaaca gccgccggcg cttatcaagg cgttcgatgt tgtgcagaac atcccgcagc
     2041 tcgcagccgc gacctcgagc ttcggctacc tcaacatcat cccggacgag gatgggacgg
     2101 tccggtgggt gccgctcgct gttcagtacg gcgacgaaat ctacccgcat atttccctcg
     2161 aggccgttcg ccggtacctc ggctctcccc cgctcatcct gaacgccgct tcctacgggg
     2221 tcaactccat ccagatcggc cggctcgtta tcccgacgga cgaatggggc aggctcctcg
     2281 tcaattaccg ggggccgcag aagaccttcc cccactattc cattatcgac gtgctggacg
     2341 gcacggttcc gaaagaagcg ctgaaggaca agattgtcct cgtcggggcc accgccatcg
     2401 gcatctacga catgcgggtc acgccctttt ccgggacctt ccccggcgtg gagatccacg
     2461 cgaacacaat cgataacatt ctgagaaccg acgcgatcaa gagacccgac ttgataacgc
     2521 tctttgatgt gaccgccatc ct
//
LOCUS       DASSLD010000215         4798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1422582, whole genome shotgun sequence.
ACCESSION   DASSLD010000215 DASSLD010000000
VERSION     DASSLD010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422582"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..1022
                     /gene="ftsE"
                     /locus_tag="VFK23_03950"
     CDS             279..1022
                     /gene="ftsE"
                     /locus_tag="VFK23_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET7318272.1"
                     /translation="MIQLYRVTKYYEGVPALSNVSFSVDKGEFVFVTGQSGAGKTTLL
                     KIIFCAEHADEGQIIIQGLNTFRVKASSIPYLRRNMGFVFQDYKLLPQKTIFENIALA
                     LKVVGAPYGEIERRAFAVLKKVGMQDKRHLTPPKLSGGEQQKVAVARALVNEPQVLLA
                     DEPTGNLDQQSAQEVFRLFRDINLKGTTVLVATHDWETVRKMQRRIITMERGKVTDDG
                     RQAGRKPAVQPAAKPDECAAGASPREGEA"
     gene            1019..1906
                     /locus_tag="VFK23_03955"
     CDS             1019..1906
                     /locus_tag="VFK23_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HET7318273.1"
                     /translation="MRGYGPFFFLSEAFHGLRANSMVNLLAVGTISMAMLIVGFFLIV
                     FFNVQSTLNNLGDRLEMSVYLKDGLTEREKEFLLHRIKSEPGVRQASLLTKAEALAQF
                     KKGLKGQEALVEGLGENPLPDSFELNIDRRYAGADRIEAMAKKFSAYVGVEDVTYGKQ
                     GAAMISALYRLLAYGGTALAVLLGVSVVFIISNSIRLALYSRGEEIELMQWIGATRAF
                     VQIPFLIEGALLAVLGSALAIGILAVLYSLLPQEIVLFLSKSNGLDFLPPSVVAYMII
                     GGGLLGLAGGLVSVSSFLE"
     gene            2010..3191
                     /locus_tag="VFK23_03960"
     CDS             2010..3191
                     /locus_tag="VFK23_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET7318274.1"
                     /translation="MKGPRLFLCAALLALGAVIAASPGFGEGGPSETKQEFERIKREM
                     KEKKKEIKRAQSREHSVLSALEKIDRDILAGSTEFANQRKRLEEAETAEREIETSSER
                     LGRELAEMKRVYAMRLRALYRMSRSGTAVIDPSDGLDGVIKQVKYLRCIAERDRTIIR
                     DYSNALMRMRMQRADLAQKKEEILRSRRAVEAQKTELEAKRRQKSAILTAVRQQKGLQ
                     EQTLRELEEASASLWAMIGTDERERRSAAARKPAAGPHEEGAPGPRGRLPWPAEGPLL
                     TRFGMQRHPQFGTMVFRRGIEIEVREGETVRAVEDGQVAYADWYRGYGKLVILDHGGG
                     LYTLYGNLSKLDLKKGQRVSRGQAIGLAGETGSLKGAKLYFEIRRKGEAEDPLLWLAK
                     K"
     gene            3292..4704
                     /locus_tag="VFK23_03965"
     CDS             3292..4704
                     /locus_tag="VFK23_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET7318275.1"
                     /translation="MQNIPEDDEMIRINKKKFLFAALVILLTGVLIGQQMVQAKSDPY
                     EDLRNFTQALEIVKRNYVENPDSRQLIEGALRGMVSSLDPHSSYMTERQFKEMNMDIK
                     GEFTGVGIQIGVKNQQLTIIAPIEDTPGFRAGLAAGDKIMKINDEWTKDMSIEQAVDK
                     MRGPKGTSVKLMIFRESWDKPREFSIVRDVIKVVSVKSKMLDDGVGYVKIIQFQGQTA
                     DELEKALKGLEAKGMKKMVLDLRNDPGGLLDSSVDVSGKFLPKDSLVVYLQGRQKTDR
                     KDFTTTSSQTPRNYPVVVLVNTGSASASEIVAGALQDSKRALIVGTQTFGKGSVQTVF
                     PLDGGGLRLTTAKYYTPSGRSIQNVGITPDIEVKLPTVKEPKDGENVHVVVREKDLDR
                     HLKNETVTEEKKKKEQPAGGEDFAMEVMPKEDKDDIQMQKAIEILKPAVLAADVFKNV
                     PPPVKKTAESEKDNVNKETD"
BASE COUNT         1122 a         1325 c         1522 g          829 t
ORIGIN      
        1 tccgcgtcgg ggtaaaggat tccttcggca cctccggcga ctacgagggg ctcatgaggc
       61 attacggcct ctccgcggaa cacatcgccg cagcggtgaa ggaagtcata aagaagaagc
      121 gctgatatgc gggacaggaa aattccagat cctaagcatc aagtctcaaa taagctccaa
      181 aaaaccaaat gataaattcc aagcatttcg gtcattggag ctttgccatt tggtatttat
      241 ttgggatttg gatattgtga tttgagattt gatccacaat gatccaactc taccgggtga
      301 ccaaatacta tgaaggcgtt cccgccctct ccaacgtgag tttcagcgtg gacaagggag
      361 agttcgtatt cgtgaccggc cagagcgggg ccggcaagac caccctcctc aaaatcatct
      421 tctgcgcgga acacgccgac gaaggacaga tcatcatcca gggattgaac accttccgcg
      481 tgaaggcctc gagcattccg tacctccgga gaaacatggg gttcgtgttc caggactata
      541 aactcctccc ccagaagacc atcttcgaaa acatcgccct ggcgctcaag gtcgtgggcg
      601 cgccgtacgg cgagatcgag cggcgggcct ttgcggtcct gaaaaaggtc ggcatgcagg
      661 acaagcggca cctcacgccc cccaagcttt cgggcgggga gcagcagaag gtggccgtgg
      721 cccgtgctct cgtgaacgag ccccaggtgc tccttgctga cgagcccacg ggaaacctgg
      781 accagcagag cgcccaggag gtgttccgcc tgttccggga catcaacctg aaaggcacga
      841 cggtcctcgt ggcgacccat gactgggaga ccgtaaggaa aatgcagcgg cggatcatca
      901 ccatggagcg gggaaaggtg acggacgacg ggcggcaggc cggacggaag cccgcggtgc
      961 aacctgctgc gaagccggat gaatgcgcag ccggagcgtc cccgagggag ggagaagcat
     1021 gagaggatac ggcccgttct ttttcctctc ggaggccttc cacgggctcc gcgccaacag
     1081 catggtgaac cttctggctg tcggcaccat cagcatggca atgctcatcg tcggtttttt
     1141 cctgatcgtt tttttcaatg tccagtcgac gctcaacaac ctgggggacc ggctcgagat
     1201 gtcggtgtac ctgaaggacg gcttgacgga gcgcgagaag gaatttctcc tccaccggat
     1261 caagtcggag ccgggagtca ggcaggcctc cctgctgacc aaggccgagg cgctcgccca
     1321 gttcaaaaag ggcctgaagg gccaggaggc gctcgtcgag ggcctgggtg aaaatccgct
     1381 gccggactcc ttcgagctga acatcgaccg ccggtatgcc ggggcggacc ggatcgaggc
     1441 catggcgaag aaattctcgg cctatgtcgg cgtagaggac gtgacctacg ggaagcaggg
     1501 cgcggccatg atatcggccc tctacaggct cctcgcctac ggcggcacgg ccctggcggt
     1561 ccttctcggg gtgtccgtcg tatttatcat ttcgaactcc atcaggctgg cgctctactc
     1621 gcggggagag gagatagagc tgatgcaatg gatcggggcg acccgggcgt tcgtccagat
     1681 cccgttcctg atcgagggcg cgctcctcgc ggtactcggc tccgcactcg cgatcgggat
     1741 cctcgccgtg ctgtattccc tgctgccgca ggaaatcgtg ctcttcctgt ccaaatcgaa
     1801 cggcctggac tttttgcctc cgtcggtggt agcatatatg attatcggag gcggcctgct
     1861 gggtctggca ggaggcctgg tctccgtaag cagtttcctg gagtagccct cttcatcgca
     1921 gcggcccgtt ccgggcccgg ccgaagggat aacaaccggt gcgtccagat aggcccctac
     1981 agaaaccgcc aaagacatcc ggaaggaaga tgaaaggccc ccgcctgttc ctctgcgccg
     2041 ccctcctggc actcggtgcg gtcatcgctg cttcccccgg gttcggcgag ggcgggccat
     2101 cggagacgaa gcaggaattc gagcgcatta agcgcgagat gaaggagaaa aaaaaggaga
     2161 tcaagagggc gcagagcagg gagcattcgg tcctctcggc cctcgaaaag atcgatcgcg
     2221 acatcctcgc gggaagcacc gagtttgcca accagcgaaa gcggctggag gaagccgaga
     2281 ccgcggagcg ggagatcgag acgagcagcg agaggctcgg acgtgaactg gcggaaatga
     2341 agcgcgtcta cgccatgagg ctccgggcgc tgtaccggat gagccggagc gggaccgccg
     2401 tgatcgaccc gtcggacggc ctcgacggcg tgatcaaaca ggtcaagtac ctgcgctgta
     2461 tcgcggagcg ggatagaacg atcatcaggg actacagcaa cgcgctcatg aggatgcgca
     2521 tgcagcgggc ggacctggcg cagaaaaagg aggagatact ccggagcagg cgggccgtag
     2581 aagcgcagaa aacggagctc gaagcaaaga gacggcagaa gtccgcgatc ctgacggccg
     2641 tgcggcagca aaagggattg caggagcaga cgctgcggga actcgaggag gcatccgcaa
     2701 gcctctgggc catgatcggg accgacgaac gggagcggcg ctccgccgcg gcgcgaaagc
     2761 ccgccgcagg acctcatgaa gagggggcgc ccggccctcg aggcaggctg ccctggcccg
     2821 ccgaggggcc gcttttgacg cggttcggca tgcagcggca cccccaattc gggaccatgg
     2881 ttttccgcag gggaatcgag atcgaggtgc gcgagggcga gacggtccgc gctgtcgagg
     2941 acggacaggt ggcatacgcc gactggtacc ggggatacgg caaactcgtg attctcgacc
     3001 atggcggcgg tttatacacg ctttacggga acctctcgaa actcgatctt aaaaaggggc
     3061 agagggtttc ccggggccag gcaatcggcc tcgccggtga gacgggaagc ctgaaaggcg
     3121 cgaaattgta ctttgagatc cggcggaagg gggaggcaga ggaccctctc ctgtggctgg
     3181 cgaagaaata aatgccgccg cacccgtgtt cagcggccga ataatttttt tgcattttgc
     3241 aggcagtcat gttatttttg atatatattc tgcttattag ggagatgaaa tatgcagaac
     3301 attccggagg atgatgaaat gattcgcatc aacaagaaaa aattcttatt tgccgcactg
     3361 gtgatactgc tgaccggggt cctgatcggg caacagatgg tgcaggccaa gtccgatccc
     3421 tacgaagacc tgaggaactt tacccaggcg ctggagatcg tcaagcgcaa ctacgtggaa
     3481 aacccggact cccggcagct gatcgagggc gccctccggg ggatggtgtc aagccttgac
     3541 ccgcactcgt cgtatatgac cgagcggcag ttcaaggaaa tgaacatgga catcaagggc
     3601 gagttcacgg gcgtcgggat ccagatcggc gtcaagaacc agcagctgac gatcatcgcc
     3661 ccgatcgagg acacacccgg attccgggcc ggtctcgcgg ccggcgacaa gatcatgaaa
     3721 atcaacgacg agtggaccaa ggacatgtcg atcgaacagg cggtcgacaa aatgagaggc
     3781 cccaagggca cgtccgttaa gctcatgata ttccgggaga gctgggacaa gccccgcgag
     3841 ttctcgatcg tccgcgatgt catcaaggtt gtcagcgtca agtccaagat gctcgacgac
     3901 ggggtcggct atgtgaagat aatccagttc caggggcaga cggcggatga actggaaaaa
     3961 gcgctgaagg gcctcgaggc aaaggggatg aaaaagatgg tcctcgacct tcggaacgat
     4021 ccgggcggcc tcctggactc atctgtggat gtgagcggaa agttcctgcc caaggacagc
     4081 cttgtggttt atctgcaggg caggcagaag acggaccgca aggactttac gacgaccagc
     4141 tcgcaaacgc cccggaacta tcccgtggtc gtgctggtga acacgggcag cgcctccgcg
     4201 tccgagatcg tcgccggagc gctccaggac tccaaacggg ccctcatcgt gggaacacag
     4261 accttcggca aggggtcggt ccagaccgtg ttcccgctcg acggcggcgg cctcaggctg
     4321 acgacggcca agtactatac gccgagcggc cgctcgatcc agaatgtcgg catcacgccc
     4381 gatatcgagg tgaagctgcc gacggtcaag gagccgaagg acggtgagaa cgtccacgtg
     4441 gttgtgcgcg aaaaggacct cgatcgccac ctgaagaacg agacggtcac cgaggagaaa
     4501 aagaagaaag agcagcccgc aggcggggaa gactttgcca tggaggtcat gccgaaggaa
     4561 gacaaggacg atatccagat gcagaaggca atcgagatcc tgaagcccgc cgtgcttgct
     4621 gccgacgtgt tcaagaacgt accccctccg gtgaagaaaa ccgcggaatc ggaaaaggac
     4681 aacgtcaata aagaaactga ttagcagctg acgcaattca aaaaaaagtt ataccaaatg
     4741 aaagcaggat gttatatcct gcttttattt tttttaatgg ggaaaaatca gatatggt
//
LOCUS       DASSLD010000216         2653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_366246,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000216 DASSLD010000000
VERSION     DASSLD010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366246"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFK23_03970"
     CDS             <1..271
                     /locus_tag="VFK23_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965927.1"
                     /note="part of a complex that catalyzes the cleavage of
                     acetyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HET7318276.1"
                     /translation="SKVPSWLAVMDNEGLSVLTAWAAGKFTASKIAQFITESGADKNI
                     NHKELIIPGYVAVLSGALEEKLPGWKITVGPREANQLPTFLKARA"
     gene            386..2587
                     /gene="acsB"
                     /locus_tag="VFK23_03975"
     CDS             386..2587
                     /gene="acsB"
                     /locus_tag="VFK23_03975"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HET7318277.1"
                     /translation="MSKIIATGAILGSHYYVKQAEALVEKALSEKGADFKFEFPDTAY
                     FLPQMFTMTGFEVRTVGDMKTALEKHVKPLLTDVPEDTLYKPYLGEALDAGMAALFAQ
                     EMIMAVRYIYGQEPVRDDSIGLTYHGFISDTILRNLGIQLVDGSMPGYVAIIGAADND
                     DQALEMARDLQQKNILTLMCGNVNGNSFTKQLLRKGVQLGWDTRLVPLGPEVEHAIYA
                     LNWAARAGITFGGMKGGDYQKILKYSKDKVFAFAMVLGPLNDRIWTTGAGAINMGFPA
                     VANTDIPVVHPTGVTIYEEVEKELDPKKIIQRCFEVRGLKITVSKPPIPVAFGPAFEG
                     ERIRKEDMHIEFGGQRTPAFEWLRTVDLGAIEDGKVIIVGTDADARYQKGGQMPLGVL
                     VEVAGRKMQKDFEPVLERKIHHFVNEAQGIWHMGQRDQNWMRVSKAALNEGYVLKHFG
                     DILTTQLKHKFNNIVDKVQVTLYVDDADVKKLLAGAQKAYLERDVRLASMTDESVDTF
                     YSCILCQSFAPNHVCVVSPERLGLCGAYNWLDAKAAYEIDPNGANQPVLKGETKDPVK
                     GRWAGVDEYVYKNSHQALEYFNAYSIMDAPMTSCGCFECIVAIVPEANGVMVVNRGYT
                     GMTPIGMKFSTLAGTVGGGAQSPGFMGVGRFFLTSKKFLSAEGGFKRIVWMTKNLKES
                     FAEQFKTRAEEEGVPDLLDKIADETVAEDSEKLVAFLTEKGHPALTMESMF"
BASE COUNT          587 a          800 c          821 g          445 t
ORIGIN      
        1 cagcaaggtg ccgtcgtggc tcgcggtcat ggacaacgag gggctgtcgg ttcttacggc
       61 atgggctgca ggcaagttca ctgccagcaa gatcgcgcag tttatcaccg agagcggcgc
      121 ggacaagaat ataaaccaca aggagctcat cattcccgga tacgtcgccg tcctctccgg
      181 cgcgctggag gaaaagctgc cgggctggaa gatcaccgtc gggccgcgcg aggcaaacca
      241 gctcccgacg ttcctgaagg caagagcgta aatactgtca accgcagagg acgcagagag
      301 cgcagaggaa tcctcgaagc gactcacgtt tctccgcgac cgcggcctcc gcggttaaaa
      361 aatagtgtca caaaaggagt gaaccatgtc aaagatcatt gcgaccggag ccatcctggg
      421 ctcacactac tacgtcaagc aggcagaagc actcgtcgag aaggccctca gcgagaaagg
      481 ggcggatttc aagttcgagt tcccggacac cgcatatttc cttccgcaga tgttcaccat
      541 gaccgggttc gaagtgcgga ccgtcgggga catgaagacc gctcttgaga agcacgtgaa
      601 gccgcttctc accgacgtac cggaagacac gctgtacaag ccctatctgg gagaagcgct
      661 cgacgcgggc atggcggccc tctttgccca ggagatgatc atggccgtcc gctacattta
      721 cggccaggag ccggtgaggg acgactccat cggactcacg tatcatgggt tcatctccga
      781 cacgatcctc cggaatctgg gcatccagct cgtggacggc tccatgccgg gatacgtcgc
      841 tatcatcggc gcggcggaca acgacgacca ggccctcgag atggcccgcg acctccagca
      901 gaagaacatc ctcaccctca tgtgcggaaa cgtcaacggt aatagtttca ccaagcagct
      961 cctccgcaag ggcgtgcagc tcggctggga cacccgcctg gttccgctcg gaccggaagt
     1021 ggagcacgca atctacgccc tgaactgggc ggcccgcgcg gggatcacct tcggcggcat
     1081 gaagggcggc gattaccaga aaatcctgaa atactccaag gacaaggtgt tcgccttcgc
     1141 catggtgctg ggccccctga acgaccggat ctggaccaca ggcgccggcg ccatcaacat
     1201 gggcttcccg gcggtggcga acacggacat cccggtggtg catccgaccg gcgtcacgat
     1261 ctatgaagag gtcgagaaag agctcgatcc caagaagatc atccagcggt gcttcgaagt
     1321 gcgcggtctc aagatcacgg tctcgaagcc gccgatcccc gtggccttcg gccccgcgtt
     1381 cgagggcgaa cgcatccgca aggaagacat gcacattgag ttcggcggcc aacgcacgcc
     1441 ggccttcgaa tggctccgca ccgtcgatct cggcgccatc gaggacggca aggtgatcat
     1501 cgtcggcacg gacgcagacg cccggtacca gaagggcggt cagatgccgc tcggggttct
     1561 ggtcgaggtc gccggccgga agatgcagaa ggacttcgag ccggtcttgg agcgcaagat
     1621 ccaccatttc gtgaacgagg cccagggcat ctggcacatg ggccagcgcg accagaactg
     1681 gatgcgggtg agcaaggccg cgctgaacga aggatacgtg ctcaagcact tcggcgacat
     1741 cctgacgacc cagctcaagc acaagttcaa caacatcgtg gacaaggtcc aggtgactct
     1801 ctacgtggac gacgccgacg tgaagaaact gctcgcaggc gcgcaaaagg cttacctcga
     1861 gcgcgacgtg cgtctcgcat ctatgaccga cgagtcggtc gataccttct actcctgcat
     1921 cctctgccag agctttgcgc cgaaccatgt gtgcgtcgta tcgccggaac ggctcggcct
     1981 ctgcggcgcc tataactggc tcgacgccaa ggcggcgtac gagatcgacc cgaacggagc
     2041 gaaccagccg gtcctcaaag gcgagaccaa ggacccggtg aaggggcgct gggcgggggt
     2101 tgacgaatac gtgtacaaga actctcacca ggcgctcgaa tacttcaatg cctactcgat
     2161 catggacgcg cccatgacct cctgcgggtg cttcgaatgc atcgtggcca tcgtgcccga
     2221 ggccaacggc gtgatggttg tgaaccgcgg gtataccggc atgacaccaa tcggcatgaa
     2281 gttctcgacc ctggccggca ccgtgggcgg cggcgcccag tcgccgggct tcatgggtgt
     2341 gggccggttc ttcctcacga gcaagaagtt cctctcggcc gagggcggat tcaagcgaat
     2401 cgtctggatg accaagaacc tcaaggagtc ctttgccgag cagttcaaga cacgggccga
     2461 ggaagagggc gtgcccgatc tcctcgacaa gatcgccgat gagacggtag cggaggattc
     2521 ggaaaagctg gtggcgtttc tgaccgagaa aggccatccg gcgctgacga tggagtcgat
     2581 gttctaacat tcgaaaacgg gtagcataac gatgcgcccc ttcgggggcg ctttacttct
     2641 cgaaggccgg atg
//
LOCUS       DASSLD010000217         2843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1473815, whole genome shotgun sequence.
ACCESSION   DASSLD010000217 DASSLD010000000
VERSION     DASSLD010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473815"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..878)
                     /locus_tag="VFK23_03980"
     CDS             complement(39..878)
                     /locus_tag="VFK23_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7318278.1"
                     /translation="MIETHELTKFFNGLGAVQSVTFRVEKGEVFGLLGPNGAGKTTTI
                     RMLTTLLAPTSGTAAVAGHDVTADPLGVKRSIGVVPQMLNLDIDLTCSENLEYHGRLH
                     QMRPNDRRARTEELLRFVGLWEKKDTPVEHLSGGMRRRLLIARGLMHRPAVVFMDEPT
                     VGLDPQARRMIWGLIEALKRGGITILLTTHYIEEADALCDRVAIMRAGRIIALDAPGV
                     LKDGVGKFAVECLGRYDIPRRFLQTREAALEAGRDMCKDVVIRDVTLEDVFISLTGER
                     IEG"
     gene            complement(1001..2380)
                     /locus_tag="VFK23_03985"
     CDS             complement(1001..2380)
                     /locus_tag="VFK23_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7318279.1"
                     /translation="MSNETILIVDDEESVRKSLADVMRDEGYEVVTAGSAREGLDLVN
                     EAQPALVLLDIAMPEMDGIETLRRIREMRPDLPVVMVTGHGTIETAVKTTKMGAYDFL
                     VKPPELAHLTLVVKHGLEEHRLREENETLKRSMERKSEIVGESGKIKALKQQIALAGP
                     TNGWVLIHGESGTGKEIVARAIHRASRRSSGPFIEVNCAAIPQELIESELFGHERGSF
                     TGATGMKRGKFELADDGTIFLDEIADMSLATQAKVLRVLEGQEFQRVGGVKTLKADVR
                     VIAASNKNLADEIKKGAFREDLYYRLNVIPLEVPPLRERADDIPRLVRHFLTEFSSEY
                     RQKPKDIDEEALSLFVRYPWPGNVRELRNFIERLIIMVPGPVLAARDVPPPVSGAPLA
                     AKQQDVALPVRPRAATLRDARAEFEREFISQKLREFGGNVSKTADAIDVERSNLHRKI
                     KALGIEVED"
     gene            complement(2513..>2843)
                     /locus_tag="VFK23_03990"
     CDS             complement(2513..>2843)
                     /locus_tag="VFK23_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7318280.1"
                     /translation="RLGRRLFDGLKQAGLKVHLNGHPEKKLPNTWSISFEGFVATEVM
                     EVLSGVAVSPGAACHGDLVEPSHVLSAMGINPALARGAIRFSLGRETTEAEIDTVVEM
                     LRKNLKR"
BASE COUNT          490 a          927 c          816 g          610 t
ORIGIN      
        1 gttattccgc attccgcatt ccgaattccg caatggattc atccctcaat cctctccccc
       61 gttaagctga tgaacacgtc ttccagcgtg acatcgcgta tgacgacgtc cttgcacatg
      121 tcgcggccgg cctcgagcgc cgcttccctg gtctggagga accgccgcgg gatgtcgtat
      181 cttccgagac actcgaccgc gaactttccg accccgtcct tgagcacgcc cggcgcgtcg
      241 agcgcgatga tccttcccgc ccgcatgatg gccacgcggt cgcagagcgc gtccgcctcc
      301 tcgatgtaat gcgtcgtcaa gaggatcgtg atgccgcccc tcttgagggc ctcgatgagc
      361 ccccagatca tccgccgcgc ctggggatcg agccccacgg tcggttcgtc catgaacacg
      421 acggcgggcc ggtgcatgag cccccgcgcg atcaggagcc ttctgcgcat cccgcccgac
      481 agatgttcga cgggcgtgtc cttcttctcc cagaggccga cgaaacgcaa gagttcctcg
      541 gtccgcgcgc gcctgtcatt cggcctcatc tggtggaggc gcccatgata ttcgaggttc
      601 tcggagcagg tcaggtcgat gtcgaggttc agcatctgcg gcacaacgcc gatcgagcgc
      661 ttgacgccca gggggtctgc ggtcacgtca tggccggcaa cggccgcggt gccggaagtc
      721 ggcgcgagca gcgtcgtcag catgcggatc gtcgtggtct tgcccgcacc gttcggtccc
      781 agaaggccga agacctcgcc tttttccacg cggaaagtca ctgactggac cgcaccgaga
      841 ccattgaaga atttggtgag ttcgtgggtt tcgatcatga gatgatatac actaccacgg
      901 attgacgcgg acggataaaa cctttgaagt ctggattccc gataaaagca ttcgggaatg
      961 acggtttttt taattccgca ttccgcaatc cgcattcggt ttagtcctcc acctcgatcc
     1021 ccagtgcctt gatcttgcga tggaggttgc tgcgctccac gtctatggcg tcggcggtct
     1081 tgctcacatt cccgccgaac tccctgagtt tctgcgagat aaactcgcgc tcgaactccg
     1141 cgcgggcgtc cctgagcgtc gcggcgcgag gacggacggg taaagccacg tcctgctgct
     1201 tcgccgccaa cggtgcaccg ctgaccggcg gcggcacgtc ccgcgccgcc agaacgggac
     1261 cgggcaccat gatgatcagc cgctcgatga agttccgcag ctcgcgcaca ttgcccggcc
     1321 acgggtaccg gacaaagagc gaaagcgcct cttcgtcgat gtccttgggc ttctgccggt
     1381 actcgctcga aaactccgtc aggaagtgac gcaccagccg cgggatatca tctgcgcgct
     1441 cgcggagcgg cgggacctcg agagggatca cgttcagacg gtaatagaga tcctcgcgga
     1501 aggccccctt ctttatttcg tcggccaggt tcttgttcga tgccgcgatt acccgcacgt
     1561 cggccttgag cgtcttcacc cctccgaccc gctggaactc ctgcccttcc agaacgcgca
     1621 agaccttggc ctgcgtggca aggctcatgt ccgcgatctc atccaggaag atggtaccgt
     1681 cgtctgcaag ctcgaacttt ccgcgcttca tgccggtggc gccggtgaac gagccccttt
     1741 cgtggccgaa aagctcgctc tcgatgagtt cctggggaat ggcggcgcag ttcacttcga
     1801 tgaaaggccc cgaagaccgt ctgcttgccc gatggatggc gcgcgcgacg atttccttgc
     1861 ccgttccgct ttcgccgtgg atcaggaccc agccgttcgt gggccccgcg agcgcgatct
     1921 gctgcttgag cgccttgatc ttcccgctct cgccgacgat ctcgctcttc cgttccatgc
     1981 tccgcttgag cgtctcgttc tcctcgcgga ggcggtgctc ttccaggccg tgtttgacga
     2041 ccagggtcag gtgcgccagt tctgggggct tgaccaggaa gtcgtaggcg cccatcttcg
     2101 tggtcttgac cgcggtctcg atggtcccgt gcccggtaac catgaccacc ggcaggtcgg
     2161 gcctcatctc gcgtatcctc cggagcgtct cgatcccgtc catctcgggc atggcgatgt
     2221 ccaggagcac gagcgccggc tgcgcctcgt tcacgagatc gagcccttct ctggccgagc
     2281 ccgcagtcac gacctcgtag ccttcgtcgc gcatgacatc ggcgagggac ttgcgcacgc
     2341 tttcctcgtc gtcgacgatg agtatggttt cattggacat aatttttaat aatgccgaaa
     2401 acgccggata taaccgcaga gtcgcggagg aagcagagaa aactcatcgg ctttcccaaa
     2461 tcctttctct gcgtctcagc ggtctatttt gacttctcag ggcaattcgt ttttatcgtt
     2521 tcagattttt ccggagcatt tcaacaaccg tatcgatctc cgcctccgtc gtctcccttc
     2581 cgaggctgaa gcgtatcgcg ccccgggcga gcgctgggtt gatacccatg gcggagagca
     2641 catgtgacgg ttcgacgagg tccccgtggc acgctgcacc cggggacacg gccacgccgg
     2701 agagcacctc cataacttcg gtcgcgacaa acccctcaaa gctgatgctc caggtgttcg
     2761 gcagcttctt ttcgggatgt ccgttcaaat ggactttcaa cccggcctgc ttcaatccat
     2821 caaataatcg gcggccaagc ctg
//
LOCUS       DASSLD010000218         4600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1515187, whole genome shotgun sequence.
ACCESSION   DASSLD010000218 DASSLD010000000
VERSION     DASSLD010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515187"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..684
                     /locus_tag="VFK23_03995"
     CDS             61..684
                     /locus_tag="VFK23_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318281.1"
                     /translation="MRISIFRYLLSTFVLALMLLPSCSPTTKLASVWRDNGYQNHPKK
                     MMIIGELKNPGNRRLFEDEMVKQLKALGTDAVVSYAAIPERTGVDRDTIAAKMNEFGA
                     DAVLIARVVDKKTVSTYVPGTIRPGYPGYGGGWHGYYAYSQSYTVQDEFAVLQTNLYD
                     LKADKLIWTAVSETWITENNESLIRTFAKVIIDRLVSDKMLAAGPAK"
     gene            737..1357
                     /locus_tag="VFK23_04000"
     CDS             737..1357
                     /locus_tag="VFK23_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318282.1"
                     /translation="MKSRIIHAASVAIISFGLLASCTGTKLVAQWKDDAYQGYTAKIF
                     VIGLSKEYGPRTLVEDEFVHQLKARGNDAIASYTVFPREEKPDKEVVLKKVQELGADV
                     IIVVKFLKKEVGDTHTPVRRYAVPQGFDTSWDSYYGGVSTDVGVRDVSYDYDAITMET
                     TLFQTATRKPIWSALSQTTYQGGPMKQIKPFTTAIVKNLVQERLIR"
     gene            1359..2012
                     /locus_tag="VFK23_04005"
     CDS             1359..2012
                     /locus_tag="VFK23_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318283.1"
                     /translation="MNRSVLVTILLCVLLVSIGCASMQVKQVWIDETYQGGQPKNVLI
                     IAVMRQPTTRRMMESEFVKHFKMKGIGAVESFRALTTDTLEGDAARDGIVAAVKDRGI
                     DAVLTVRSAGSRIEERNIPGMTITTGVGMPYGSYGAWGGYTTVMASFPEPSSPTTQGY
                     SAEQKFLSLETQLYDVRTEKLIWSALSEMRISGPPQEEIKDFVSLMSRELFRAKPFR"
     gene            complement(2049..>4600)
                     /locus_tag="VFK23_04010"
     CDS             complement(2049..>4600)
                     /locus_tag="VFK23_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HET7318284.1"
                     /translation="TVTLQHIDVSLLPRPRLIVRAVGISFRGTLTGTLESAHIYPELL
                     PLLRGRIHIAKVLLERPDLVMEFSEHRERRQEKKRPVPPGEQRAKIASAIASIRSLAP
                     ELVAEVDEGRLAVTVDNKQVMFVRGLQARLALPPNGFDIAITGNVDHWGTISASGRFL
                     STDNDLLEIKGLSLSGGRSSLSDLSGRVSWEKAPSLKITSGRSVIFLQDLTERLSSVE
                     AVHNALKSFHTLKGTINLTSLTYAGQLLHPEHGTIEASGSVANVIVDAAILPGPVKVN
                     SGTFKATMDSVSVTDARAELLDASFVTSILISGVSHNEYSIDVSVTGNTGPATVRWAS
                     AKFDLPPKLTLRAPLSLSQTRFRRQKDGKSSLRGTLKVQNGPLVSTDIHWDERELLIK
                     RIDIQDEGSKASITLHRRGKLLDVSFAGSIHEQTLNRLFDQSSFRHGWIQGDLKAHVA
                     LDRPQESAAEGSLEGKDLLIPWTFKTPLEIKSIRLSARDKTVTIGSSDLTLANTHFTL
                     AGTVTASARQYIVDGDIGASTVVIDTLQKALEDDAKPGGPGAKPDGALQKKPLPLQGT
                     LRCKVDNLSYGKFVLNPVQATVVLAPDEIRVDHLEATFCGITVRGGLSLAGGDISVGL
                     KPDAKGLQLEPALTCLAGKDVKISGVFDLSGTLSARGKPDALVSSLQGSAEFTAREGH
                     FYRSPTLAKILSLLNVSDLLRGNLSDFRAKGLSYRSISIRAGIRSGKLDLQELVIDSS
                     AMNIVGQGVIDLKDSTVSMTVLVAPFTTVDKIVSKIPVVNYILEETLVSIPVTMSGRI
                     SDPEVKFMPASAVGDGVLGILKRTLKAPVKIIEPVLPGERKKEGASDPREG"
BASE COUNT         1044 a         1237 c         1352 g          967 t
ORIGIN      
        1 cgcagtgtgc ccgagccatg cacgctcctc caaggcaggg attttcaaag gaggcaacac
       61 atgaggatct caatattccg ttatctcctg tcaacgttcg tgctggcact gatgcttctg
      121 ccgtcctgct caccaacgac aaaacttgcc tctgtttgga gggataacgg ataccagaat
      181 catccgaaaa aaatgatgat cattggggaa ctcaagaatc cggggaaccg aaggcttttc
      241 gaggacgaga tggtgaaaca gcttaaagct ttggggaccg atgccgttgt cagctatgcc
      301 gcaatccctg agcggaccgg ggtagatcga gacacgatcg ccgcgaaaat gaacgagttt
      361 ggggctgatg ccgtgctcat cgcacgggtt gtggacaaga agacggtgtc gacctatgtc
      421 cctggtacga tcaggcctgg ctatccaggg tacgggggcg ggtggcacgg ctactatgcc
      481 tacagtcaaa gctatacggt ccaggacgaa tttgccgttt tacagacgaa cctctatgac
      541 ctcaaggccg acaagctcat ctggaccgcc gtgtcggaga cctggattac ggagaacaac
      601 gagagcctga tacggacgtt cgccaaggtc attatcgatc gtttggtaag tgacaagatg
      661 ctcgcggcag gaccagcaaa atagcgaacg aacggccggc ccaggaaagg acctgccacg
      721 gaaaggaaac agcgtcatga aaagcagaat catacatgcg gcaagcgtcg cgatcatttc
      781 tttcggtctg ctagcctcat gtaccggaac gaagctggtc gctcaatgga aggacgatgc
      841 ataccagggg tatacggcga agattttcgt catagggctt tcgaaggagt acgggccccg
      901 gacgctggta gaggatgagt tcgtacatca gctcaaggcg cgcgggaacg acgccatcgc
      961 aagctacact gtgttcccgc gcgaggaaaa accggataag gaagtggtcc taaaaaaggt
     1021 acaggaactg ggcgccgacg tgatcatcgt ggtgaagttc ctcaaaaaag aggtgggaga
     1081 cacgcacacc cccgtccgac ggtatgccgt accgcagggt tttgacacga gctgggacag
     1141 ctattacggc ggtgtttcga ccgatgtggg cgtgagggac gtgtcctacg attatgacgc
     1201 gatcaccatg gaaacgaccc tcttccagac tgcaacacgc aagcccatct ggtcggcgct
     1261 gtcccagacc acctaccagg gcggtcccat gaagcagatc aaaccgttca ccaccgcaat
     1321 tgtgaaaaat cttgttcagg aaaggctgat ccgatagcat gaacaggagc gtattggtca
     1381 ctatcctact ctgtgtgctg ctggtgagca ttggttgcgc ctcaatgcag gtgaagcagg
     1441 tttggatcga tgaaacctac cagggaggac agccgaagaa cgtccttatc atcgcggtga
     1501 tgaggcagcc cacgaccagg aggatgatgg aaagcgagtt cgtgaagcat tttaaaatga
     1561 aggggatcgg cgcagtggaa agcttccggg cccttacgac cgatacgctg gaaggtgacg
     1621 cggcgcgcga cggcatcgtc gctgctgtca aggatcgggg gatcgacgcc gtcctgacag
     1681 tcagatctgc cggcagccgg atcgaagaac ggaacatccc gggcatgacc atcacgaccg
     1741 gcgttggcat gccatatggg tcctatggcg cttggggtgg ttataccacc gtcatggctt
     1801 ctttcccgga gccgtcctcg ccgacgacgc agggatactc tgcggagcag aagttcctgt
     1861 cccttgaaac ccagctgtac gatgtacgga ctgagaagct catctggtcg gcattgtcgg
     1921 aaatgcgcat ctccggcccg ccgcaggaag agatcaagga ctttgtctcg cttatgtcca
     1981 gagaactgtt cagggcgaaa ccatttcgct agctccactc cgaactgtac cgttaaaaaa
     2041 cttccctctc acccttctct cggatcactg gccccttctt tttttcgctc tcccggcaag
     2101 accggttcga ttatcttgac cggggccttc aaggtccgct tcaggatgcc gagcacgcca
     2161 tcaccgacgg cagatgcggg catgaacttc acctcagggt cgctgattct gccgctcatg
     2221 gtcacgggta tggaaacgag ggtttcctcg aggatatagt tcaccacggg gatcttgctg
     2281 acgatcttgt caacggtcgt aaagggggcc actagcaccg tcatgctgac cgtggaatcc
     2341 ttcagatcga tcacaccctg gccgacgatg ttcattgcgg aagaatcaat gaccaattcc
     2401 tgcaggtcga gcttcccgct tcggataccg gccctgatcg agatcgatcg ataggaaagc
     2461 cctttggccc ggaagtcgga aaggtttccc ctcagcaggt ccgatacgtt cagcagcgac
     2521 aggatctttg ccaacgtggg tgatcggtaa aaatgaccct cccgggcggt aaactcggcc
     2581 gatccctgga gcgaagaaac aagagcatca ggttttccgc gggccgagag ggtaccgctc
     2641 agatcgaaga caccgcttat tttcacgtcc ttcccggcaa ggcaggtgag cgcaggctcc
     2701 agctgaagtc ctttcgcatc aggcttgagg ccaacgctga tgtccccgcc ggcgagtgaa
     2761 agaccgccac gcacggtgat cccgcagaac gtggcctcaa ggtgatcgac cctgatctca
     2821 tcaggggcca gaacaactgt tgcctgtaca gggttgagaa caaatttgcc gtaggagagg
     2881 ttgtccactt tgcaacggag agtgccctgc aggggcagcg gttttttttg caatgctccg
     2941 tccggcttcg ctccaggacc acccggtttc gcatcatctt ccagcgcttt ctgcaatgta
     3001 tcgatgacga ccgttgatgc gccaatgtcc ccatcgacaa tgtattgtcg cgccgatgct
     3061 gtgacggttc cggcaagggt gaagtgcgta ttcgccagcg tgagatcgga cgatcctatg
     3121 gtaacggtct tgtcgcgggc gcttagccga atacttttga tttctaatgg cgttttaaag
     3181 gtccagggaa tgaggagatc tttgccctcg aggcttccct ctgcagcgga ttcctgtggc
     3241 cgatccagcg cgacatgggc cttgaggtcg ccttgtatcc atccatgacg gaacgaggac
     3301 tggtcgaaca agcggttcaa agtttgttcg tgtatgcttc ccgcaaaaga gacatcaagg
     3361 agcttgcccc gtcggtgaag cgtgatggaa gccttggacc cctcgtcctg gatgtcaata
     3421 cgtttgatca gcagttcccg ctcatcccaa tggatatccg tagagaccag agggccgttc
     3481 tgcactttca gggtgccgcg gagcgacgat ttgccgtctt tctgccttcg gaaacgcgtc
     3541 tgcgaaagcg aaaggggggc acggagggtg agcttaggcg gcaggtcgaa tttcgctgaa
     3601 gcccaccgta ccgtagcagg accggtgttt cccgttacgg atacatcaat cgagtactcg
     3661 ttatgactga ctcctgaaat aaggatggat gtcacaaaag acgcgtcgag aagctccgcc
     3721 cgggcatcgg ttacggaaac gctgtccatc gttgccttga acgtgccgct gttgaccttg
     3781 accggaccgg ggaggattgc tgcatcgaca atgacattcg cgacgctccc tgaagcctcg
     3841 atggtcccgt gctcaggatg gaggagctga cccgcatagg taaggctggt gaggttgatg
     3901 gtgcctttca gggtatgaaa cgatttcagt gcattgtgga cggcttcgac ggatgaaagc
     3961 cgctccgtaa ggtcctgcag gaagatcacc gatcttcccg aggtgatctt gagagagggg
     4021 gccttctccc aactgacccg accggagaga tcggaaagcg atgagcgacc ccctgacagg
     4081 gagaggccct ttatctcgag gaggtcgttg tctgtcgaca ggaatcggcc acttgccgat
     4141 atcgttcccc agtggtcgac gttgcccgtg atcgcgatgt cgaagccatt cggcgggagc
     4201 gcaaggcgtg cctgcaggcc cctgacgaac attacctgtt tattgtcaac agtaacggca
     4261 agacgtccct cgtccacttc ggcgaccagc tcaggagcga gggaccggat cgaggcgatc
     4321 gcggaagcga tctttgcccg ttgctccccc ggtggcacag gccttttctt ttcctgcctc
     4381 cgttcacgat gctcggagaa ctccatgaca agatctggtc gttccagcag taccttcgcg
     4441 atgtgtatcc gtccccgaag aagaggaaga agctccggat agatatgcgc cgattccagc
     4501 gtgcccgtca gcgtaccgcg gaatgagatg ccaaccgcac ggacaatgag tcgggggcgt
     4561 gggagtagag agacatcgat gtgttgtaag gtaacggtat
//
LOCUS       DASSLD010000219         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_626183,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000219 DASSLD010000000
VERSION     DASSLD010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626183"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFK23_04015"
     CDS             complement(<1..655)
                     /locus_tag="VFK23_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HET7318285.1"
                     /translation="MKTVFLYSERFAGFDYGSGHPLKPMRLKLTCELIKACGLLDSPD
                     PRIVEPPAASVEDLLAWHTAEYVDILKAANGGTYVPGAEEFGLGPGDNPIFAGMFDWS
                     RLIAGASLMAADLVDSGEAVVAFNISGGLHHAMSGHASGFCYVNDPVLAIKLLIDRGR
                     RVAYIDIDAHHGDGVQEAFYATDRVLTISLHETGRVLFPGSGFEQETGTGAGKGYSVN
                     "
     gene            complement(875..1696)
                     /locus_tag="VFK23_04020"
     CDS             complement(875..1696)
                     /locus_tag="VFK23_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HET7318286.1"
                     /translation="MARFKTPLEIYALLPKTNCGECGTATCLAFAAAILKEEKQPADC
                     PRLDEGALARLGSGVGRQVNMESIRDEQLKALKDKISEQDINSRAGLLGARSNGKCVT
                     IRCLGKDFEVDGKGAVSSQCHTHAWFTLPLLDYILHCEGAEPSGRWVPFRELPRGKTW
                     NPLFERRCEKPLKRIADGHSDLFEALVAMFSGTSTGRALDSDISVVLFPLPRVPIMIC
                     YWRPEDGMESELHLFFDDTAERNLPVESLFTMATGIVRMLERIMYRHTDGKSELD"
     gene            2084..>2812
                     /locus_tag="VFK23_04025"
     CDS             2084..>2812
                     /locus_tag="VFK23_04025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HET7318287.1"
                     /translation="MTAIAGKIRSKPSALLEGAVPGAMDAAALDKFGPPQVLSLHTLP
                     VPVPGQGEVLIELHAAGVGVWDASVRDGSWRPFDRPKFPLIPGTDGAGVVVMKGPRVR
                     RFDIGDRVWAYHYANPKGGFYAEYTAVDVEHVGSMPRRLNFLEAAAGATTGLTALQGI
                     DDALRVAKGETVLIFGATGAVGTLAIQFAKRKRARVLATASGKEAAALAKKLGADGIF
                     DARGDDALERLKALAPDGINAALVL"
BASE COUNT          572 a          835 c          857 g          548 t
ORIGIN      
        1 ggttgacgct gtatcccttc ccggctccgg ttccggtctc ctgctcaaac cctgatcccg
       61 gaaaaagcac gcgtcctgtt tcatgcagag aaatggtcag gacccggtcc gtcgcgtaga
      121 atgcctcctg cacgccgtcg ccgtgatggg cgtcgatgtc gatataggcg acccttctcc
      181 cccggtcaat caggagcttg atcgcaagaa cagggtcgtt gacatagcag aacccggagg
      241 catgtcccga catggcatga tggagcccgc ccgagatatt gaacgcgacc acagcctctc
      301 cgctgtcaac gaggtcggcc gccatgaggg aagctcctgc gatgagcctc gaccaatcga
      361 acatgccggc aaagatcggg ttgtctccgg gaccgagccc gaactcttcg gcgccgggaa
      421 cgtacgtacc gccgttcgcc gccttgagga tgtcgacata ttccgcggtg tgccacgcga
      481 gcaggtcctc gacactcgct gcaggcggct ccacgatccg cgggtccggg gagtcgagca
      541 ggccgcatgc cttgatcagc tcacaggtca gcttcagccg catgggcttc aagggatggc
      601 cggacccgta atcgaacccc gcgaaccgtt cggagtatag gaagacggtt ttcacggatt
      661 aactctgtct ttattttacc tctcaaatca tagtccggcg caaagtcgtt aaaccgcaga
      721 ggacgcagag gggaaagcaa ggcgagggag tcggaagatt taggccgtgg aaccgcgaat
      781 ggcgcgaatt aacgcgaaga gacaagcagt cattagcatg cggtcgcgtt cattagcggt
      841 gaagccttgg gtttaaaacc agtcatcgag ccggtcagtc cagctcgctc ttgccgtctg
      901 tgtgccggta catgatccgt tcgagcatgc gcacgatccc ggtcgccatc gtgaaaagcg
      961 actccacggg gaggtttcgc tccgctgtgt cgtcgaagaa gaggtgaagc tccgactcca
     1021 tgccgtcctc cggcctccag tagcaaatca tgatcggcac cctgggcaga ggaaagagga
     1081 caacgctgat gtcggagtca agcgcgcggc ccgtggaggt cccgctgaac atagccacga
     1141 gggcctcgaa caggtcgctg tggccgtccg cgatccgctt gagaggcttc tcgcagcgcc
     1201 gctcgaagag cgggttccag gtcttgcccc tcggaagctc gcgaaaaggc acccacctgc
     1261 ccgagggctc cgccccctcg cagtggagga tataatcgag aagaggcagg gtgaaccatg
     1321 catgcgtgtg gcactgcgac gagacagcgc ccttgccgtc gacctcgaag tccttgccca
     1381 ggcacctgat cgtaacgcac ttgccgttac tcctggcgcc gagcagccct gcccgggaat
     1441 taatgtcttg ttccgagatc ttatccttga gcgccttcag ctgttcatcc ctgatgctct
     1501 ccatgttgac ctgcctgccg accccgctcc cgagccgggc gagcgccccc tcatccaggc
     1561 gcgggcagtc ggccggctgc ttttcttcct tcaggatcgc ggcagcaaag gcaaggcagg
     1621 tcgctgtgcc gcattctccg caattcgtct tcgggaggag cgcatagatc tcaagagggg
     1681 ttttgaaccg tgccatgcga catggtaccg ggtttcagcg cataaggcaa ccgggaagta
     1741 cgaagggaca ttcggccgtg cccgcgggct catcctttcg tccaattgac gtgcgacatc
     1801 cgctcttaca tactttaatc agaatttaga gcaaggctat ttaaccacgg agaacgcaga
     1861 gggaaaacaa ggcgagggat tcaaaagatt cagatttcgg atttaaaacc gcgaatggcg
     1921 cgaattagcg caaatagacc cgcaatcatt agcgtgtgtt tgcgttcttt agcggttagg
     1981 catcggttta aacaaaacat caaagccgtt gccatcctct tttctcatct ctcggtgacc
     2041 tcggtggtga atgattttga taataccaga gggaggtatt cctatgacag ctattgcggg
     2101 taaaatacgt tccaaaccat cagcattact ggaaggggcg gtccccggag cgatggacgc
     2161 ggccgccctg gataagttcg gaccgccgca ggtcctctcg ctccatactc ttccggtccc
     2221 ggtgcccggg cagggtgaag tgctgatcga gcttcacgcc gccggtgtgg gcgtgtggga
     2281 cgcctcggtg cgcgacggct catggcggcc gttcgaccgt ccgaagtttc cgctcatccc
     2341 gggaacggac ggcgctgggg tcgtggtcat gaaaggaccg cgcgtcaggc gcttcgacat
     2401 cggcgaccgc gtgtgggctt atcattatgc caatccgaag ggcgggttct acgcggaata
     2461 cacagcagtg gatgtcgagc acgtgggaag catgccccgg aggctcaatt ttctggaggc
     2521 ggccgccggc gcaaccacgg gactgaccgc gctccagggg atcgacgacg cgcttcgtgt
     2581 cgctaagggc gagaccgtgc tcatcttcgg ggccacggga gcagtgggca ctctggccat
     2641 ccagttcgcg aagcgcaagc gcgcccgtgt cctggccaca gcttcgggca aagaagcggc
     2701 cgcgctcgcg aagaagctcg gagcggacgg aatattcgac gcccggggcg acgatgcgct
     2761 cgaacggctc aaggccttag cgcccgacgg tatcaatgcg gccctcgttc tt
//
LOCUS       DASSLD010000220         2852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_105848,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000220 DASSLD010000000
VERSION     DASSLD010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105848"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VFK23_04030"
     CDS             <1..828
                     /locus_tag="VFK23_04030"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7318288.1"
                     /translation="HLRQAEAFFRAGSRPRFDVTRAEVDVNTARLGMINAGNDVRLRT
                     IALYNAMGTDPKGDIEIEDILSRPTAFPSLDQMQAEALNNRPEMQKATADIQAAQARV
                     RAAESNYLPTLSANGAYNWAHGRSETTLPAGPLGGGEFQSDIRNSWNAGIVLSMPLFE
                     GGLTQGRVSEARANLRALEAQRDGLRQSILLEVNQAFADLDSASARISVMESSRQKAK
                     ENLELAEGRYQAGISQSLEVTDAQVAYIKAETDNAQALYDYQLAAARLAKATGKRDL"
     gene            971..1429
                     /locus_tag="VFK23_04035"
     CDS             971..1429
                     /locus_tag="VFK23_04035"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7318289.1"
                     /translation="MPSKEVIFTINLPVKSVWSFMTDRLEVGCLFPGCKGVKILNDLD
                     SVWTIKMSLGPFSRMIEMHAHTTEMVEQKRMAWTATHEHLITSGIVTFRKVSEEETEI
                     TYHLEGHVTGHFSFLQEIVVAEKLGEVCRSFMKNIKARLEYKAEQDPKGS"
     gene            1431..2528
                     /locus_tag="VFK23_04040"
     CDS             1431..2528
                     /locus_tag="VFK23_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7318290.1"
                     /translation="MALLLKLAKNKFVWIAVCAVLIVALIAMNAKKPVAVRIVELKPG
                     ELRVVINATTTSTVKSETEVTLSAQRTGRVVKLPITEGDVVKAGALIAQLDLTEESVQ
                     TANVLAQSKATYDEAEKNLKRSEDLFNKGMISQQDFDAVRRAYDVAKSQYRAAEADEG
                     VKRDYSVIRAPFRGVVAKKFTEVGELLLPGKQIVTIVDPDRIYVLATIDEVDVGRLNL
                     GQQVGITVDAFPGEKFSGRIRRISPIVSGGKLETRTADVWIYFDEKQPRIKPGMSADV
                     EILVTTLKDVLSVPSQAVIEREGKKQVFVADGSPLKPGDKTIARLRPVEIGETNWSYT
                     QIVSGIKPGEHIITTPEATGLKDKAKVKIEE"
     gene            2626..>2852
                     /locus_tag="VFK23_04045"
     CDS             2626..>2852
                     /locus_tag="VFK23_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015119128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7318291.1"
                     /translation="MIELSNITKIYRMGEVDITVLSGVCLSIKQGEFVSVMGPSGSGK
                     STLMNIIGCLDRPTSGTYRFEEREISSLSDD"
BASE COUNT          679 a          788 c          871 g          514 t
ORIGIN      
        1 catctgcggc aggcggaggc ctttttccgc gccggctcaa ggccccggtt cgatgtgacc
       61 cgcgcggagg tcgatgtcaa caccgcccgt ttgggaatga tcaatgccgg gaacgatgta
      121 cgcctgcgga ccatcgcgct gtacaacgcc atggggaccg acccgaaagg cgatatcgag
      181 attgaggata ttctttcccg gccgacggcg ttcccttcgc tcgatcagat gcaggctgag
      241 gccctcaata accggcccga gatgcagaag gcaaccgcgg acattcaggc ggcccaggcg
      301 cgcgtcaggg ctgcggagtc gaattatctg ccgaccctct ccgcgaacgg ggcatacaat
      361 tgggcccatg gaaggtcgga gacgacgctg ccggccgggc cgcttggcgg cggggaattt
      421 cagagcgaca tccggaacag ctggaatgcc ggcatcgtgc tttccatgcc gctctttgag
      481 ggcggcctga cgcaggggag agtgagcgag gcccgggcaa acctccgcgc cctcgaagcg
      541 cagcgggacg ggctccggca atcgatcctg ctcgaggtta accaggcctt cgcggacctc
      601 gacagcgcct cagcccggat ttccgtaatg gagtcctccc ggcagaaggc gaaagaaaat
      661 cttgagctcg ccgagggaag ataccaggcc ggcatcagcc agtcgctcga ggtcacggat
      721 gctcaggtgg cttacatcaa ggcggagacc gacaacgcgc aggcactgta cgattatcag
      781 cttgcagccg cgcggctggc caaggcgacg ggcaaaagag atttgtgatt gtctatggca
      841 tcataaaaaa agcgtcattc ccgaacgctt taatcgggaa tccaggggtt ctaaaaagac
      901 actgggtccc cgaaaaaggc attcggtgac gacgaactat ttaactgagg tgttaaagcg
      961 gggttaaaat atgccgtcaa aggaagtcat cttcaccatc aatctgcccg tcaagagcgt
     1021 ctggtctttc atgacggacc gcctggaggt aggctgcctg tttccgggct gcaagggggt
     1081 caagatcctg aacgatctgg attccgtctg gacgatcaaa atgtcgctgg gccccttcag
     1141 ccgcatgatc gagatgcatg cgcacaccac ggagatggtc gaacagaagc ggatggcgtg
     1201 gaccgcgacc catgaacacc tcatcacctc gggcatcgtc acgtttcgaa aggtttcgga
     1261 ggaagagacc gaaatcacct accacctcga gggacatgtg actggccact tctcctttct
     1321 ccaggaaatc gtcgtcgccg agaaactggg cgaggtatgc cggtccttca tgaaaaacat
     1381 caaagcaagg ctcgaataca aggcggaaca ggatccgaag ggatcgtagt atggctctgt
     1441 tgctcaaatt ggcaaaaaac aaattcgtgt ggatcgccgt ttgcgccgtc ctgattgtcg
     1501 cgctcatcgc gatgaacgcc aaaaaacccg ttgcggtcag gatcgtcgag ctcaaaccgg
     1561 gggaactccg cgtcgtcatc aacgcgacga cgacgtcgac ggtaaagtcg gagaccgagg
     1621 tgacgctctc ggcccagcgg acgggcaggg tcgtcaaact tccgattacg gaaggggacg
     1681 tcgtaaaggc cggggcgctc atcgcgcagc tagacctgac cgaggagtcc gtgcagacgg
     1741 caaacgtcct tgcccagagc aaggcgacgt acgacgaggc ggaaaagaac ctgaagaggt
     1801 cggaagacct gttcaacaag ggcatgatct cccagcagga tttcgacgcc gtccggaggg
     1861 cctacgatgt ggcgaagtcc cagtaccgcg ccgcagaggc cgatgaaggc gtgaaacgcg
     1921 actactcggt catcagagcc cctttccggg gagtcgtggc gaagaaattc acagaggtgg
     1981 gcgaactgct cctgccgggc aaacagatcg tgacgatcgt ggatcccgac cggatctacg
     2041 ttctggcgac gattgacgag gtcgatgtgg gaaggcttaa cctggggcag caggtgggca
     2101 tcacggtcga cgcttttccg ggtgaaaagt tctccggcag gatcagacgg atttctccca
     2161 tcgtgagcgg cgggaagctg gagacccgca ccgccgatgt gtggatctat ttcgacgaga
     2221 aacagccccg gattaaaccc ggaatgtccg ctgatgtgga aatcctggtg acaacgctga
     2281 aggacgtcct gtccgtgccg tcccaggcgg tcatagaacg cgaggggaaa aagcaggttt
     2341 tcgtagcaga cgggagcccg ctcaaaccag gcgataaaac gatcgcgagg ctccgtcccg
     2401 tggaaatcgg cgagaccaac tggagctata cccagatagt cagcggcata aaacccgggg
     2461 aacacatcat cacgacaccg gaggccacgg ggctcaagga caaggcgaaa gtgaagattg
     2521 aggagtaagt tcggagtttt gagtttgtaa ttcggagtaa aggcaggcat aattccggga
     2581 tttcactccc aactccgaac tttgaactct agactggttt aaacagtgat cgagctttcc
     2641 aacatcacca agatctaccg catgggagag gtcgacatca ccgtcctctc cggtgtctgc
     2701 ctgagcataa aacaggggga attcgtctcc gtcatgggcc cctcgggatc cggcaaatcg
     2761 acgcttatga acatcatcgg ctgtcttgac cgtcctacct ccggcacgta ccgtttcgag
     2821 gaacgggaaa tcagctcgct cagcgacgac ga
//
LOCUS       DASSLD010000221         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_137989,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000221 DASSLD010000000
VERSION     DASSLD010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137989"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VFK23_04050"
     CDS             <1..1061
                     /locus_tag="VFK23_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET7318292.1"
                     /translation="VAGLAMVLVSNADAAEKKVPQGRTLVVGTKEAPPFSMKAADGTW
                     TGISIDLWRDIAAQLNLAFEFRETDLRGLIDGVTDGSLDVAIAALTLTSEREKVMDFS
                     HVYFHTGLGIAVSKKANTPWRDVFRTFNSPAILKFVVGLFLFLLLFGSLIWRLERKKN
                     PEQFGGDMKAGIISGLWWSMVTMSTVGYGDKAPITHGGRCVAILWMLVSIFVISVLTA
                     HITSSLTLIQMRSSINGPQDLIHVRVGSVAESTSSDYLGKNRIPFYSYKTASEGLHAM
                     TEGKIQALVYDKPILQYLVQKDYPGKLEVLPYTLHEEDYGIALREGSPLREPINQVLL
                     EIINSPAWQDTLSLYLGR"
     gene            complement(1246..1788)
                     /locus_tag="VFK23_04055"
     CDS             complement(1246..1788)
                     /locus_tag="VFK23_04055"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 family protein"
                     /protein_id="HET7318293.1"
                     /translation="MAGGSENPDTRRNFILQQGLDAPAQEGCPVRFSALMFQPRLVGL
                     FTLLAVILQSPTIFLVLSGILWWNALIPRLNLFDVVYNRTLANRSGAVRLEPAPPPRR
                     FAQGMAGSFALTIGVLLLLQLGTAALVVQVLLLAALAALIFGRFCLGSFLYHLLRGRS
                     DFAIGTLPWKPGSSHGSPSK"
     gene            complement(2060..2398)
                     /locus_tag="VFK23_04060"
     CDS             complement(2060..2398)
                     /locus_tag="VFK23_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318294.1"
                     /translation="MAGSWAGRTGVSEPQPREGDDWETRFEAWTRRMRVDLPAFRAYA
                     KAVYEATDAYLAALSDAELSREVDLSTMGLGMQPVRFVLDNILLGHAYCHCGEISALK
                     GIQGKKGYPF"
BASE COUNT          549 a          686 c          708 g          485 t
ORIGIN      
        1 tggtcgcggg actggcgatg gtcctggttt ccaacgccga tgccgcagag aagaaggttc
       61 cacagggccg caccctggtg gtcggaacga aagaggcgcc acctttttcc atgaaggctg
      121 ccgatggcac ttggaccgga atcagcatcg acctctggcg cgacattgcc gcacagctga
      181 acctggcctt cgagtttcgc gagacagacc ttcggggcct catcgacggg gtcaccgacg
      241 gttctctgga tgtcgccatc gccgcgttga ccttaacctc ggaacgggag aaggtcatgg
      301 acttctctca cgtctacttt cacacgggcc tcggcatcgc cgtctccaaa aaggccaata
      361 ccccctggcg agatgtcttc cggaccttca actcccccgc gattctgaaa ttcgtagtgg
      421 ggctgtttct gtttttgctc ctgttcgggt ccctcatttg gcggctcgaa cggaagaaaa
      481 acccggaaca gttcggcggc gacatgaagg cggggatcat ttcgggtctc tggtggtcta
      541 tggttaccat gagcaccgtc gggtacggag acaaggcccc tatcacccac gggggacgat
      601 gtgtggcgat cctctggatg ctcgtcagca tcttcgtgat ttcggttctc accgcgcata
      661 tcacttcgag cctcacgttg atacagatga ggtcgtcgat caatgggccc caggacctga
      721 ttcatgtacg cgttggctcc gtcgcggaat caaccagcag cgactacctg ggaaagaacc
      781 ggataccgtt ctattcctac aaaacggcat ccgaggggct ccacgcaatg acagagggaa
      841 aaatccaggc gctggtgtac gacaagccga tccttcagta cctggttcag aaagattacc
      901 cgggaaaact ggaggtcctg ccatacacct tacacgagga ggattacggg atcgccctgc
      961 gggaggggag tccactgcgc gaaccgatta accaggttct gctggaaatc atcaattcgc
     1021 cagcgtggca ggacaccctt tccctatatc tgggccggtg aaagaaggta atcaaagggg
     1081 gagttcccgt atgaccaatg aacagatcag accggaagag gctcgggagc ggaaggccct
     1141 ctggaagaag tgggacccta cctttgtgaa ccactgttgg gaacagtgta cggctttggg
     1201 tgggataaca ttcaccgcgg tgagtggcca tgataaaaac gctggtcact tcgaggggga
     1261 gccgtgagac gatcccggtt tccacggaag agtgcctatc gcgaaatcgg atcttccgcg
     1321 aaggagatgg tataggaacg aacctaagca gaaccggccg aaaatcagtg cggcaagtgc
     1381 cgcaagtaac aggacctgca cgacgagggc agccgtcccc agttggagca gaagcagcac
     1441 accgatggtc aaggcgaagg aacctgccat cccttgggcg aatcggcgag ggggcggagc
     1501 aggctccagg cggacggcgc ccgacctgtt tgccaaagtc cggttgtaga ccacatcgaa
     1561 gagattcagg cggggaatga gtgcgttcca ccaaaggatc ccggacagga ccagaaagat
     1621 cgtgggcgac tggaggatga ccgccagaag ggtgaacaac ccgaccaatc gcggttgaaa
     1681 catgagtgcg gagaaccgga cgggacaacc ctcctgcgct ggtgcatcca gtccttgctg
     1741 gagaatgaaa ttcctcctgg tatccggatt ttccgatccg cctgccatga tctctcctgt
     1801 aaccaatcct ctttccattc gataacccga gggacatcgt cgattcgctc tctgtttcat
     1861 gcgaggaaag aacggtagga gtaacatgga gtactttgga aaactcgcga actgcctccc
     1921 tattgagcca actggacgag gtgaatttca tggggggcca tacagaccgc aaaccccgca
     1981 aacaactcgc ttttctgggt gaaaagaggg ttttgctgtg acgctgttga cggggaaacc
     2041 acttctttaa gaggagttct cagaacgggt accctttctt tccctgtatc cccttgaggg
     2101 ccgagatctc gccgcagtgg caatacgcgt gaccgagtag tatgttgtcc aggacaaatc
     2161 tgaccggttg cattcccaat cccatcgtgg acagatccac ctcccgcgat agttcggcgt
     2221 ccgatagagc ggcaaggtag gcgtcggttg cctcgtagac cgccttggca tatgcccgga
     2281 acgccggcag atccacccgc atccgtcgag tccacgcctc gaaccgggtt tcccagtcgt
     2341 ccccctccct cggctgcggc tcggataccc cggtccttcc ggcccaggat cccgccataa
     2401 gcggtgcgac tcccttgaac agggaatg
//
LOCUS       DASSLD010000222         3585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_684828,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000222 DASSLD010000000
VERSION     DASSLD010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684828"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VFK23_04065"
     CDS             <1..750
                     /locus_tag="VFK23_04065"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET7318295.1"
                     /translation="LVATMTPDMIMPSAGCVLQDKLGARKAAAFDIYAACSGFIYGMA
                     VANAFIRSETYRNVLLVGAEVLSRFMDWEDRTTCILFGDGAGAVVLQRYAGKRGILST
                     HLHSDGSMSDLIHIPAGGSQHPASHDTIHKRMHFIKMKGNETFKAAVRALEGVVQETL
                     KHNRVKSEDIDFLVPHQPNLRIIQAMAQRLNMPMEKVVLTLPKHGNTSAASIPMALDE
                     AVRDGRIRDNNLVLFEAFGGGLTWASALVRW"
     gene            891..2048
                     /locus_tag="VFK23_04070"
     CDS             891..2048
                     /locus_tag="VFK23_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7318296.1"
                     /translation="MDYLLTEEQIMIRDLARQIAVEKIVPVRAELDEHNKFPADIMKA
                     MAQADLFGIYIPEEYGGLGKKSLELCIAVEELSRACVGVSTSFAANALGTYPILLHGS
                     DAQKKKYLPDIAAGRKYVAFGLTEANAGSDASGVRTTGKLDGNEYVLNGTKQWITNGG
                     EADVYTVIAITDKAKGPRGASAFIVEKGTPGFTFGKKENKMGIRASSTTELIFNNCRI
                     PRENLIAKEGMGFIVAMKTLDSSRTGVGAQGVGVAQGAIDEALQFAKQRVQFGQPVAA
                     FQAVQHMLADMQTQTEAARSLVYSVARYIDSGAKDVSRASAMAKLFATDVAMKVTTDA
                     VQVMGGSGYMKEYPVEKMMRDAKILQIYEGTNQIQRNVIALDMIKQASKAK"
     gene            2141..2926
                     /locus_tag="VFK23_04075"
     CDS             2141..2926
                     /locus_tag="VFK23_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET7318297.1"
                     /translation="MHIIVLVKQVPDASEVKINRETNTLIRDGVPSIINPFDTYAIEE
                     ALRLREKHGGKVTAVTMGPPQAAEALKEAVSLGVDDVVLLSDRAFAGADTWATSYVLS
                     QGIKKIGAYDLVIAGKQAIDGDTAQVGPETADMLGIPFVAYIRKIDAVEGKKMTAERL
                     MDEGYDVVETSLPALITVVKEINQPRLPSLKGKMKAKNLKVAGWTAGDIGADPNKCGL
                     KGSPTKVVKIFPPAPRGQREILSGSLEEKVATVVRKLKEQSLI"
     gene            3033..>3585
                     /locus_tag="VFK23_04080"
     CDS             3033..>3585
                     /locus_tag="VFK23_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET7318298.1"
                     /translation="MSVRIISDKCECCETCVTACPFGAIEMRDGKAFITEACTMCGAC
                     VEVCEFKAIERTEEAAGPAADLSAYKGVWIFAEQHRGGIASVALELLGEGRKLADKRK
                     AKLSAVFIGSGIRDKAQELIARGADIVYVADDPALKDFNDDSYAAILTTLAHQHKPEI
                     ILAGATAIGRSFFPKVAAALATGL"
BASE COUNT          816 a         1073 c         1065 g          631 t
ORIGIN      
        1 ctggtggcca cgatgacgcc ggacatgatc atgccaagcg ctggatgcgt gctccaggac
       61 aaactcggcg ccaggaaagc cgccgctttc gacatttatg ccgcctgttc cggcttcatt
      121 tacgggatgg ccgtcgcgaa cgcgttcatc aggtctgaaa cgtaccgaaa cgtccttctc
      181 gtcggagccg aagttctgtc gcggttcatg gactgggagg accgtacgac atgcatcctc
      241 ttcggggacg gcgcgggcgc ggtggtgctc cagcggtacg cgggcaagcg cggcattctc
      301 tcgacccatc tgcattccga cggatcgatg agcgacctga ttcacatccc cgccggaggg
      361 tcccagcatc cggcctccca cgacaccatc cacaagagaa tgcacttcat caagatgaag
      421 ggcaatgaaa ccttcaaggc tgcggtgcgg gccctggaag gcgtggttca ggaaacgctg
      481 aagcacaaca gggtcaaatc ggaggacatc gatttcctcg tgccgcacca gcccaatttg
      541 aggatcatcc aggccatggc ccagcgtctg aacatgccca tggaaaaggt cgtgctcacg
      601 cttccgaagc acggaaacac ctcggcggcg tcgatcccca tggccctcga tgaagcggtg
      661 cgggacggca ggatacggga caacaatctc gtgctgttcg aagccttcgg gggcggcctg
      721 acctgggcct cggcattggt gagatggtag aaaaaaaaga taagcacaaa atttcaaaca
      781 ccaaattaca aataaatccc aaacgtcaat aatcaaaatc aaacgtcgga attgtaaatc
      841 tggaatttgg aatttatttg ggatttggaa ttttcctaag ggggttttta atggattacc
      901 tgctgaccga agaacagatc atgatacgcg atctcgcccg acagatcgcg gtggaaaaga
      961 tcgtgcccgt gcgcgccgaa ctcgacgagc acaacaagtt ccccgcggac atcatgaaag
     1021 ccatggccca ggcggacctg ttcggcattt acatacccga ggaatacggc ggtcttggga
     1081 aaaaaagcct cgagctctgc atcgccgtcg aagaactgtc ccgggcctgc gtgggcgtct
     1141 cgaccagttt cgcggccaat gccctcggga cctaccccat cctgctccat ggatccgacg
     1201 cgcagaagaa aaaatatctt cccgatatcg cggcgggcag gaaatacgtg gcattcgggc
     1261 tcacggaggc gaacgcgggc agcgacgcga gcggcgtgcg gaccacggga aaacttgacg
     1321 gcaacgaata cgtgctgaac ggcacgaagc agtggatcac gaacggcggc gaggccgacg
     1381 tctacaccgt catcgccatc accgacaagg ccaagggccc ccgcggtgcg tcggccttca
     1441 tcgtcgaaaa agggaccccc ggcttcacct tcggcaaaaa ggagaacaag atgggcatcc
     1501 gcgcctcttc cacgaccgag ctcatcttca acaactgccg catcccccgg gaaaacctga
     1561 tcgccaagga aggcatgggg ttcatcgtcg ccatgaagac gctcgacagc tcccgcaccg
     1621 gcgtgggcgc gcagggcgtg ggcgtcgcgc agggggccat cgacgaggcc ctgcaattcg
     1681 cgaagcagcg ggtgcagttc ggccagcccg ttgcggcctt tcaggccgtc cagcacatgc
     1741 tcgcggacat gcagacccag accgaggcgg cccgttcgct cgtatactcg gtcgcgcggt
     1801 acattgactc gggggcgaag gacgtttccc gggcgtccgc catggccaag ctttttgcca
     1861 ccgacgtcgc gatgaaggtg accacggacg ccgtgcaggt catgggcggt tcgggctaca
     1921 tgaaggaata ccccgtcgag aagatgatgc gcgacgcgaa gatcctccag atctacgaag
     1981 gcaccaatca gatccagcgc aacgtcatcg ccctggacat gatcaagcag gcatcgaagg
     2041 cgaaataacg gccgttggtc atgagtcatt agtcatgagg acttcgactc ttgacttctg
     2101 actattgact gttggctgtt gactcttttc ggagaaaagc atgcacatca tcgttttagt
     2161 caaacaggtc cccgacgcgt cggaagtcaa gatcaaccgc gagacgaaca cgctgatccg
     2221 cgacggcgtg cccagcatca tcaatccctt cgacacgtat gccatcgagg aggcgcttcg
     2281 cctgcgggaa aagcacggcg gcaaggttac cgctgtcact atgggtccgc cccaggccgc
     2341 cgaggcgctc aaggaggctg tttccctcgg cgtggacgat gtcgtcctcc tctcggaccg
     2401 ggcatttgca ggcgctgata cgtgggccac gtcctacgtt ctctcgcagg ggatcaagaa
     2461 gatcggcgcc tacgacctcg tcatcgccgg caaacaggcc attgacggcg acacggccca
     2521 ggtgggaccc gagaccgccg acatgctcgg cattcccttc gtcgcctaca tcagaaagat
     2581 cgatgcggtc gaagggaaaa agatgacggc cgagcgactc atggacgagg gatacgacgt
     2641 ggtagagacc tctctccccg cactcatcac cgtcgtgaag gagatcaacc agccgaggct
     2701 tccctcgctc aagggcaaga tgaaggcaaa gaacctcaag gtggcgggct ggaccgccgg
     2761 cgacatcggc gccgacccga acaagtgcgg cctcaagggc tccccgacaa aggtcgtcaa
     2821 gatctttccg cccgccccgc gcggccagcg ggaaatcctc tccgggagcc tggaggaaaa
     2881 ggtcgcgacg gtcgtccgga agttgaaaga acaatcgtta atctaatgcg gaatgcggat
     2941 ggtggaatgc ggaatgaaat actcataatg agtcgttcct gcccttccct ctgcgtcctc
     3001 tgtggtgagc tgtttttttc aaggagcaac ccgtgagcgt tcgaatcata tcggataaat
     3061 gcgagtgctg cgaaacatgc gtgaccgcgt gtcccttcgg agccatcgaa atgagggacg
     3121 gcaaggcctt catcaccgaa gcatgcacca tgtgcggggc ctgcgtcgag gtctgcgagt
     3181 tcaaggccat cgagaggacg gaggaagcag ccggccccgc cgccgacctt tccgcgtata
     3241 agggcgtgtg gatcttcgcg gagcagcacc ggggcggcat tgccagcgtg gcgctggaac
     3301 ttctcggaga aggaaggaaa cttgccgata aaagaaaagc caagctgtcg gccgtattca
     3361 tcggttctgg catcagggac aaggcgcagg aactcatagc ccgcggcgcc gatatcgtgt
     3421 atgtcgccga cgatcccgcg ctcaaggact tcaatgacga ctcctacgcc gcgatcctga
     3481 cgacgctcgc gcaccagcac aagcccgaga tcatcctcgc gggcgccacg gccatcggcc
     3541 ggtccttctt ccccaaagtc gcggccgcgc tcgcgaccgg cctca
//
LOCUS       DASSLD010000223         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_554836,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000223 DASSLD010000000
VERSION     DASSLD010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554836"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1850
                     /locus_tag="VFK23_04085"
     CDS             <1..1850
                     /locus_tag="VFK23_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7318299.1"
                     /translation="GEIDLMPDVAYTAERERQYSFHKIPVLSVWSQVYAPRNSGIQSI
                     LDLNGKRVAALEQTIQLETFERLTNSFGLKITLVPVPDYRTEFEMIAAGKVDAGVTNR
                     FYGLMYARKFRLEDTPIMFDPAPFFFAAPRAASRELLDVIDRRLSDMKSNPNSAYYAS
                     LKRWTSEEVQFKLPAWLKILGLALGAATVLTLIVVIRRQHAAETALRKEKAFADTIID
                     SVPGAFYVIDEAGRLVRWNRYLSKLNDLSGEELRGMDSIRNIHEDDKNILARSIAEAF
                     STGEAEAEARIRTREGERYFLFTGRRIQAGGSPLVVGSGIEITGRKRAEQELVQYKEH
                     LEELVEERTTTLQETNAKLQREIGERSRAQDELRAALDDLAVAKERAEAADRLKSAFL
                     ATMSHELRTPLNSIIGFTGILRQGLAGPLNEEQRKQLAMVQNSADHLLALINDVLDIS
                     KIEAGQLQVASEPFSLRGSIQKIVQAVQPLAGKKGLKLTAEIAVGVEVITSDQRRVEQ
                     ILLNIVSNAIKYTERGEVRIACGMRENSVSTTVADTGIGIAKEDLAKLFMPFYQVESG
                     LTRRYEGTGLGLSICKRLVELLGGTIRVESELGRGSSFSFTLPVQRRAS"
     gene            1847..2230
                     /locus_tag="VFK23_04090"
     CDS             1847..2230
                     /locus_tag="VFK23_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7318300.1"
                     /translation="MNKKILYIEDNAQNLYLVTYILEKNGYEVVGARDGQEGIAAASR
                     ELPALILLDIQLPGMDGYTVARKLRDKPELVGIPIVAVTSYAMVGDRERALGAGCTGY
                     IEKPINPETFLTQIERHLPSGGPLP"
BASE COUNT          501 a          640 c          707 g          397 t
ORIGIN      
        1 cgggcgaaat agacctcatg cccgacgtgg catataccgc ggagcgtgaa cggcaatact
       61 ccttccacaa aatccccgtc ctgtccgtct ggtcccaggt gtatgccccc cgaaatagcg
      121 ggatccagtc catattggac ctgaacggaa agcgtgtcgc cgccctggag cagacgatac
      181 agcttgagac cttcgagcgg ctcacgaaca gcttcggact gaagatcacc ctcgtgccgg
      241 tgccggatta caggacggag ttcgaaatga ttgccgcggg caaagtcgat gccggcgtga
      301 ccaaccgttt ctacgggttg atgtatgcaa gaaaattcag gctggaagac acgccgatca
      361 tgttcgatcc agcgcctttt ttcttcgcag cgccccgggc ggcttcccgc gaattgctgg
      421 acgtcatcga ccgccgtctt tcggacatga agagtaaccc caactctgcg tactatgctt
      481 cgctgaagcg atggacgtcc gaggaggtgc agtttaaact gccggcatgg ctcaagatcc
      541 tcggcctcgc tctcggcgcg gcaacggtgc tcaccctgat cgtcgtgatc aggcggcagc
      601 acgccgcgga gaccgctctc cggaaagaaa aggcttttgc ggatacgatt attgacagcg
      661 tacccggcgc gttctacgta atcgacgagg cggggcggct tgtccggtgg aatcggtacc
      721 tgtcgaagct gaacgacctg tccggggagg aactgcgggg aatggacagc attcgcaaca
      781 ttcatgaaga tgacaagaac attctggcgc gttccatcgc ggaagcattc agtacggggg
      841 aagccgaagc ggaagcgcgg atccggacca gggaaggcga acgctatttt ctctttaccg
      901 gcaggcggat ccaagccgga ggaagtccgc tcgtcgtcgg cagcggaatc gagatcaccg
      961 gccggaagcg ggccgaacag gagctggtgc agtataaaga acacctggaa gagcttgtgg
     1021 aggagcggac cacgacgctg caggagacca atgccaaact gcagcgcgag atcggagagc
     1081 ggtcgcgcgc gcaagatgag cttcgcgccg cgctcgacga cctcgccgtc gccaaggagc
     1141 gggccgaggc cgcggaccgg ctgaaatcgg cgttccttgc caccatgtcg cacgagctcc
     1201 gcacacccct caactccatc atcggcttca ccggcattct gcggcagggt ctggccggtc
     1261 ccctgaatga ggaacagcga aagcagctgg caatggtcca gaacagcgct gaccaccttc
     1321 tcgccctgat caacgatgtg ctggatattt ccaagatcga ggcaggccag ctccaggtcg
     1381 cgtccgagcc gttcagcctg aggggatcga tccagaagat cgttcaggcg gtgcagccgc
     1441 tggccgggaa gaaaggattg aaacttacgg ccgagatcgc ggtgggagtg gaggtcatca
     1501 cgagcgatca gcggcgggtc gagcagatcc tcctgaacat cgtcagcaat gccatcaaat
     1561 acaccgaacg cggggaggtg cggatcgctt gcggcatgcg cgagaattcg gtcagcacga
     1621 cggttgccga tacaggcatc ggcatagcga aagaggatct ggcaaaactg ttcatgccgt
     1681 tttaccaggt tgaaagcggc ctcacgagac gctatgaggg caccggtctg ggcctgtcca
     1741 tctgcaagcg gctggtcgaa ctgctgggcg gaacaatccg ggtggagagc gaattgggca
     1801 ggggcagctc gttcagtttc accctgcccg ttcaaaggag agcatcgtga acaagaagat
     1861 cctttatatc gaggacaacg cacagaacct ctatctcgtg acgtatatcc tcgagaagaa
     1921 cggctacgag gtcgtcggag cccgggacgg acaggagggg atcgccgccg cgtcacggga
     1981 gctgccggcg ctcattctgc tggacattca gctgcccggg atggacggct atacggttgc
     2041 gagaaaactg cgcgacaagc cggagctcgt cggcattccc atcgtggccg ttacctccta
     2101 cgcgatggta ggcgaccggg agcgggccct gggagcgggg tgcaccgggt atatagaaaa
     2161 accgatcaat cccgagacct ttctcactca gatcgaacga cacctgccgt caggaggacc
     2221 tttgccatga gccgtgtgat gatcg
//
LOCUS       DASSLD010000224         4666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_837703,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000224 DASSLD010000000
VERSION     DASSLD010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837703"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="VFK23_04095"
     CDS             <1..1325
                     /locus_tag="VFK23_04095"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET7318301.1"
                     /translation="MGKITRLIILFLALPVALGLSGCGGGGSSAPPTGGLNITIIDGT
                     TANPQKGIAHARVILLDAESKPVQTYFTDNTGIVSQSKLPIGDYQLKISAQGYNSSPP
                     PRVPPLPVKVKQGETTTATIELFPIDTNLVVGAISGTVRSGGNPVAGALVIAMGAGVS
                     YTTISAADGSYVLFNVVEGNVDVTAYIKGLNFPTLSAVTVTADTTTTGQDLVASGVAN
                     GTISGNIQFVAGGTAKATDVTLIDRETREVIPGMRVYTDANNNYTMSGLPNGDFEIIA
                     SMLNDGNVIDPDEAVTQGDPLVTVTDGVVDPSTRSFKITGAVVMDTPSTPANNVVPEL
                     TATPTFTWHSESSYSSASGFAIEVINESGDAVWGGFGGLDPVVGVPTVTVSAGTYTIN
                     YAGPALQSGRYYQLRIYAMKDDNNLILYPNGYKLISVTENLDGVFKVK"
     gene            1911..2906
                     /locus_tag="VFK23_04100"
     CDS             1911..2906
                     /locus_tag="VFK23_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7318302.1"
                     /translation="MSPFIFRVRRMWLLTGTVLMLTALTPRLGHAEVARVELHTFQST
                     TLTDQEFLTGEKEGKPVILAGELRIPKPGSDRLPAVVLVHGSGGVSGYVDDWAQWFNA
                     MGVATFVFDSFTARGIVNTNTDQDQLGRLAMIVDTYRALELIAKNPRIDPERIALMGF
                     SRGGTATLYASMKRFQRMHGPAGLAFAAYIPFYPQCGVTFIDDENVADKPIRIFHGAA
                     DDYVPVAPTRLYVERLRKAGKDVQLTEYAGAQHVFDWVMLKTPMKLAQGQTARNCQLE
                     EAADGRIINSNTKQTFGWNSDSCVERGVTIGYNAQAHSEAQKAVRELVNTVLKPK"
     gene            complement(3155..3700)
                     /locus_tag="VFK23_04105"
     CDS             complement(3155..3700)
                     /locus_tag="VFK23_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HET7318303.1"
                     /translation="MKRQDVIAMTGMVLVALVAGSALTWAETSPLVPENLRPPATEVL
                     SLGTKAIGVQIYECNVSKDEPTRFEWTFKAPEADIFDMAGNKIAKHYAGPTWESNDGS
                     KVVGEVKAKDNGPDPDAIPWLLMSAKSTSGTGVFSQVKSIQRVHTVGGRAPTEACSQA
                     QAGKVARVGYKATYNFYVAKP"
     gene            complement(3874..4209)
                     /locus_tag="VFK23_04110"
     CDS             complement(3874..4209)
                     /locus_tag="VFK23_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318304.1"
                     /translation="MKTSTVGLVLAVILMVVAGIGFGIAQAGGNHKDGPELSFEDQEA
                     LGQGSSSSPYADDRPVLSFGDEMQLRNPTETGSLPDTSNTDSSVKYEIIDGVTWYRVE
                     GKLYELYGP"
     gene            complement(4407..>4666)
                     /locus_tag="VFK23_04115"
     CDS             complement(4407..>4666)
                     /locus_tag="VFK23_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318305.1"
                     /translation="RGVYQVPKNEMRISSDAGRSLNNTGRTAFPVVLEVVAMAFSAHP
                     AMANPPRANPESFNASRRVNPLDIVFLVVFMISCPSVIGLA"
BASE COUNT         1093 a         1255 c         1258 g         1060 t
ORIGIN      
        1 tcatgggaaa aataaccagg ctgataattc tgtttctggc tcttccggtg gccttggggc
       61 tgagtggctg cggtggagga ggaagcagcg cgccgcccac aggcgggctc aacatcacca
      121 tcatcgacgg gacgacagca aatccgcaga agggtattgc tcacgcccgt gtcatcctgc
      181 tcgatgccga aagtaaaccg gttcagactt actttaccga taacactggt atcgtctctc
      241 aatcaaaact gccaataggt gactaccagc tgaagatcag tgcccagggc tacaattctt
      301 ccccgccgcc gcgggtacct ccactgccgg tgaaggtgaa acagggtgag accacgacgg
      361 ctaccattga actcttcccg atagatacga atctcgttgt cggcgcgatc agcggaacgg
      421 tcaggtcggg cggtaacccg gttgctggcg cgctggtcat tgccatgggt gcgggggttt
      481 cctacaccac gatctccgcc gccgatggca gttacgtcct gttcaatgtc gtggaaggca
      541 acgtcgacgt taccgcctac atcaagggtc tgaatttccc gaccctgagc gcggttacgg
      601 tcaccgccga tacgaccacg accggccagg atctcgttgc cagcggtgtg gcaaacggaa
      661 caatcagcgg caacatccag ttcgtcgctg gggggactgc caaagccacc gatgtcacgc
      721 tgatcgaccg agagacgcgc gaggttatac cgggcatgcg cgtatacacc gatgcgaaca
      781 acaactacac gatgagcggt ctgcccaatg gcgatttcga aatcatcgcg tcgatgctga
      841 acgacggcaa tgttattgat ccggatgaag cagttaccca aggcgatccg ttggtcacgg
      901 tcactgatgg ggttgtcgat ccttccacaa gaagtttcaa aatcactgga gctgtcgtga
      961 tggatacacc ctcgactccg gccaacaatg tggttccgga gctgaccgca acacctacgt
     1021 tcacgtggca ttcggagtcc agttattcaa gtgcgagtgg ctttgccatc gaagtcatca
     1081 atgaatccgg tgacgcggtc tggggcggat ttggtggcct cgatccggtt gtaggtgttc
     1141 ccacggtgac cgtctccgca ggtacctata caatcaatta tgcaggacca gcattacagt
     1201 ccggccgtta ttaccagttg cgtatttacg cgatgaagga tgacaataat ttgatcctgt
     1261 accccaatgg ctacaagctt atctctgtca ccgaaaatct cgacggggtg ttcaaggtga
     1321 aataattgat cgcggacagc agaaaaacgg ggccttttgg ccccgttttt cttggaagtc
     1381 gatccatttc tctgtcccag gtggggagcc atataagact cgaacactta cgtgtccccc
     1441 cctcaaaatt gcgtagccta agctggattt gcattcgaca acggagacag actgaatagt
     1501 ttttgtatag tagacacccc aaaacacccc aaaacaggac aaacgtcaga aaattgacgg
     1561 catgaaaatc ttaataaaga caaaagatta ggggtcaccg ggtgttcccg gtgaccccat
     1621 ttggccggtt tcggattaag cggccggggt tcgaatccct cagggcgcgc cagcaaattt
     1681 aatccgggca tcctttttcc ggggtgcctg gatctttatt ccccactgga acccgaccat
     1741 cccaccgtag catctttaat ttaaaaaagg gcaaccgtca atcttaatgg catgccccgg
     1801 aaaccgagga gcttattaaa atggggattc gtggattgac ggagattccc ctctcttatt
     1861 attgcttgcc aataaacacc tttctactag ggaggcacga ggaagaagct atgtcccctt
     1921 tcatttttcg ggttcgaagg atgtggctgc tcaccggtac ggtcttaatg ttgacggctc
     1981 ttaccccgag gcttggccat gcggaagtgg cacgagtaga attgcacacg tttcagtcca
     2041 ccactttgac cgatcaggag ttcctgaccg gggaaaagga aggaaagcca gtcattctcg
     2101 ccggggaact gcggattcca aagcccggat ccgatcgctt gcccgcggtt gtgctggttc
     2161 atggctcggg tggcgtatcg ggttacgtgg atgactgggc tcaatggttc aatgcgatgg
     2221 gggttgcgac ctttgtattc gatagcttca cggcacgagg cattgtgaac acaaacaccg
     2281 atcaggatca acttgggaga ctagccatga tcgtcgatac ctaccgggct ctggaattga
     2341 ttgcgaagaa tccgcggatc gacccggaaa ggatagccct catgggattc tcccgagggg
     2401 gcacagccac cctctatgcc agcatgaagc gcttccagcg catgcatggt ccggctggcc
     2461 tcgcattcgc ggcatacatt ccgttctatc cgcaatgcgg ggtgacattc atcgatgacg
     2521 agaacgtcgc agacaagccg attcgaattt ttcatggcgc cgccgatgac tatgtcccag
     2581 tcgcaccgac ccggttgtat gtggagcggt tgcgcaaagc ggggaaggac gttcaactga
     2641 ccgaatacgc tggtgcacaa catgtcttcg attgggtgat gctcaagaca ccgatgaagc
     2701 tggcccaggg gcagacggca cgcaactgcc aacttgaaga ggcggccgac ggtcggatca
     2761 tcaacagtaa taccaagcag acgtttggat ggaacagcga ctcatgcgtg gagcgaggtg
     2821 tgactattgg atacaacgcc caggcgcaca gcgaggcgca gaaggcggta cgcgagttgg
     2881 tgaataccgt gcttaagccg aaatgaaggc ggcgttgcgg gcccttccac gaacccgacg
     2941 gcgtatcaag tacgatatgc gtccatgcgt ccgcgatggg gagccatgat gcgatcccaa
     3001 tattggaatt agggtaaggg gagcgtttct tcccagtagt accgggaaaa tcgccaaggt
     3061 gatcgtcttc gatatgggac acccggtggc ggcggcgacg gggtagaggg ctaccggtac
     3121 acgctccgtc accgggtcag ctgcattttc ctgcctacgg cttggcaacg tagaagttat
     3181 acgtagcttt gtagcctacc cgagccactt tgcccgcctg cgcttggcta caggcttcgg
     3241 tgggcgctct gccgccgacg gtatgcacac gttggatgct cttcacctga ctgaatactc
     3301 cggtccctga agttgatttc gcgctcatca gcaaccatgg aatggcgtcg gggtctggac
     3361 cattgtcttt ggccttcact tcaccaacca ccttgcttcc atcattcgat tcccaggtcg
     3421 ggccggcata gtgtttggcg atcttgttgc ctgccatatc aaatatgtca gcttccggtg
     3481 ccttgaaggt ccactcaaac cgtgtcggct catctttgct gacgttgcac tcgtagatct
     3541 gcacaccgat ggcctttgtc ccaagcgata gcacttcggt cgccggaggc ctcaggtttt
     3601 cgggtaccaa aggactcgtc tcggcccagg taagcgcgct gcccgcgaca agcgcgacga
     3661 gtaccatccc cgtcattgct attacatcct gccttttcat ctcagggtcc tcctgaacgc
     3721 cagggatttg aattgaattt ttccaaggtc ctgccaaaaa ggtgcctcgc accagagccc
     3781 ggttttatcc tgccgggcgc cttaaaaaga aaaaatccgg gcacctccaa caaggatgcc
     3841 cggatgcacg cgccctgtct cgcccggcca atgttatgga ccgtatagtt cgtaaagttt
     3901 tccctcaacc ctgtaccacg taaccccatc gatgatctcg tattttacgg aggaatccgt
     3961 attgctcgtg tcaggcaagc taccggtttc tgtaggattc ctaagttgca tctcatctcc
     4021 gaagctcaat accgggcgat catctgcata tgggcttgaa gaactgccct gtccgagtgc
     4081 ctcctggtct tcgaaactca gctctgggcc gtccttatga tttcctcccg cctgtgcgat
     4141 tccgaaaccg atgcctgcaa caaccatcag aatcactgcc aaaaccaacc caaccgtgga
     4201 cgttttcatt tttctccccc tcctttccgg ggttcggtta gggaacgcgg acaaatcttg
     4261 ttcgtatgat ttctcattac cagagaaagt ttcctcgatg gataaaatag aaaaggccat
     4321 ggggcaaggg aacaagcagg aaaaagtcgt ttgaggatat taataaattc cgatatatta
     4381 ttacaaggaa agagggaggg gaaacatcaa gctaatccaa tgacggaagg acaagagatc
     4441 atgaagacta ctaaaaaaac aatgtccaaa ggattgacac gacgagacgc attgaagctt
     4501 tcagggttgg ccctgggcgg gttcgccatt gcaggatgcg cagagaaggc catcgcgacg
     4561 acctccaaaa ctactgggaa ggctgttcgc cctgtattgt taagtgaacg tccggcgtcg
     4621 gatgaaatgc gcatctcatt ttttggaacc tggtatacac ctcgac
//
LOCUS       DASSLD010000225         2820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1539134, whole genome shotgun sequence.
ACCESSION   DASSLD010000225 DASSLD010000000
VERSION     DASSLD010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539134"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1865
                     /locus_tag="VFK23_04120"
     CDS             <1..1865
                     /locus_tag="VFK23_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7318306.1"
                     /translation="FFSLLVITVGFLPVFTLQAQAGRLFKPLAYTKTFAMLFSSFLAV
                     TLTPVLMTLFIRGKIRSEEQNPVSRILRRLYEPAASLALKYKKTVIVVAIVIMAATVY
                     PFMKLGSEFMPPLYEGSLLYMPVTVPPPSISVVSELLQLQDKLLMRIPEVSQVFGKAG
                     RAETATDPAPLEMFETVVNLKPESQWRPGMDVEKLKNEMNDALTIPGVANSFTMPIKN
                     RIDMLATGIRTPVGVKVLGPKLDEIERIGIEIENAIKGIKGTRSAYAERVNTGYFIDF
                     TVKREEAARYGLTVDDVQQVIQTALGGMSATTTIEGRERYTVNVRYARELRDSIDKLK
                     RVLVPVASMNAETATRNAESMGPAQTPQLTQVPLVQLADIEVVKGPTGIKSEAGLLAV
                     YVYVDFSGRDVGGYVEEAKQKVASAVKLPAGYRLEWSGEYEYLVKTRERLKIVIPVTL
                     LVIFVLIYMNTKSVTKTAIVLLAVPFSLVGSFWLLYFLNYNMSIAVWVGIIALAGLDA
                     ETGVVMLLYLELAYEQWKKEGRLRTVADLKDAVMHGAVKRIRPKIMTVSVILAGLIPI
                     MFSTGAGADIMKRIAAPMVGGVITSTVLELVIYPAIYVIWKERDLKRASGAGSP"
     gene            1862..2077
                     /locus_tag="VFK23_04125"
     CDS             1862..2077
                     /locus_tag="VFK23_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318307.1"
                     /translation="MTIRDMRKAELKKILKTVDEELTREQFPLEGRQTHALLRLSQRL
                     KTKIASHPDDEIYKLVMSWYEKDTGAR"
     gene            2232..2726
                     /locus_tag="VFK23_04130"
     CDS             2232..2726
                     /locus_tag="VFK23_04130"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HET7318308.1"
                     /translation="MVYVLNLIEKLTLEQARTLSPVELKELMLYFSWGLGAFQSINLF
                     SGHPLTREAVADLRSAVDHLFREPRACGKSKWASFQAAEKFIKGYIASRGGEADKQHG
                     LLELVSDAYSLGLRPLNPDNLEVLMCDSRVSSGEASVSVEEAVEAHHASIDICANIAS
                     SARR"
BASE COUNT          593 a          848 c          841 g          538 t
ORIGIN      
        1 tctttttttc attactggtc atcacggtcg gattcctgcc ggtgttcacg cttcaggccc
       61 aggcgggcag gctcttcaag ccccttgcct ataccaagac cttcgccatg ctgttctcct
      121 ctttcctcgc ggtcactctg acaccggttt tgatgaccct gttcatccgg ggcaaaatca
      181 ggagcgagga gcaaaacccc gtgagccgca tcctgcgcag gctctacgag cccgcggcct
      241 cactcgcgct caagtacaag aagacggtca tcgttgtcgc aattgtcatc atggccgcga
      301 ccgtttatcc cttcatgaag ctcgggagcg agttcatgcc tcccctgtac gaggggtccc
      361 tgctctacat gccggtcacg gtcccgccgc cgtccatttc cgtggtttcc gaactcctgc
      421 agctccagga caagcttttg atgcggatcc cggaggtgtc ccaggtcttc ggcaaggccg
      481 gcagggccga gacggccacc gaccccgcgc cgctcgagat gttcgagacg gtggtgaacc
      541 tgaagcccga gtcgcagtgg cgtcccggca tggacgtgga gaaactcaag aacgagatga
      601 acgacgcgct caccatcccc ggcgtggcca actccttcac catgccgatc aaaaaccgga
      661 tcgacatgct cgccaccggc atccggacgc ccgtcggggt caaggtgctg ggcccgaagc
      721 tggacgaaat cgagaggatc gggatcgaga tcgaaaacgc gatcaaaggc atcaaaggca
      781 cgcggagcgc gtacgcggag cgcgtgaata ccggctactt catcgacttc accgtcaagc
      841 gggaggaggc ggcgcggtac ggcctcaccg tggacgacgt gcagcaggtg atccagaccg
      901 ccctcggcgg catgagcgca accaccacga tcgaaggccg ggagcgatat accgtgaacg
      961 tgcgctacgc gcgggagctt cgcgattcca ttgataagct gaagcgcgtg ctcgttccgg
     1021 tcgcatccat gaacgcggaa actgcaacac gcaacgcgga atccatgggg cccgctcaaa
     1081 ccccgcaatt aacccaggtc cccctggtcc aacttgcgga catcgaggtt gtgaaggggc
     1141 cgacgggcat caagagcgag gcggggctcc tggcggtata cgtttacgtg gacttctccg
     1201 gcagggacgt gggtgggtac gtggaggagg ccaagcagaa ggtggccagc gccgtgaaac
     1261 tgccggcggg ataccggctg gagtggagcg gcgagtacga gtaccttgtc aagacccgtg
     1321 agcggctgaa gatcgtgatc ccggtcactc ttctcgtcat cttcgtgctg atctacatga
     1381 acacaaaatc ggtgaccaaa acggccatcg tgctcctggc cgtgcctttt tcgctcgtgg
     1441 ggtccttctg gcttctgtat ttcctgaact acaacatgag cattgcggtc tgggtgggga
     1501 tcattgccct cgcgggcctt gacgcggaga cgggtgtggt catgctcctc tatctcgagc
     1561 ttgcctatga gcagtggaag aaagagggaa ggctccgcac cgtggcagac ctgaaggacg
     1621 cagtgatgca cggcgcggtc aagcgcatcc ggcccaagat catgaccgtc tccgtgatcc
     1681 tcgcgggcct cattcccatc atgttctcaa ccggcgcggg cgccgacatc atgaagcgga
     1741 tcgccgcgcc catggtcggc ggcgtcatta cctcgacggt cctggagctg gtcatctacc
     1801 ctgcgatcta tgtgatctgg aaggaacggg atctgaagcg agcgtccggt gcggggtccc
     1861 cgtgacgatc cgcgatatgc gcaaggctga actgaaaaaa atcctcaaga ccgtcgatga
     1921 ggaattgacc agggaacagt tccccctcga aggccgacag acgcatgcct tgctgcgtct
     1981 gtcccagcgc ctcaagacga agatagcctc ccatcccgac gacgagatat acaagctcgt
     2041 catgtcctgg tacgaaaaag atacgggagc aaggtgaagg cggcccgaac gatcggcgac
     2101 atggctatcc tcatccgggg agagctctat aagatgtcga tccccccatc cgcggtcagg
     2161 cgcagatcgt cgtcgatccg aatgtcgaga cgaagaagcg cgttcagaaa agcagcgccc
     2221 cggaccccga cgtggtctat gtgctgaacc tgatcgagaa gctcacactc gagcaggcca
     2281 ggacattatc tcccgttgaa ctcaaggaat tgatgctgta tttcagctgg ggcctcgggg
     2341 cctttcaatc catcaatctg ttctcaggcc atcccctgac ccgcgaggcc gtagcggact
     2401 tgagaagtgc ggtcgatcat ctgttcagag agccccgggc ctgcggaaag tcaaaatggg
     2461 cgagctttca ggcggccgag aaattcatca aaggatacat cgcgtcgcgg ggaggtgagg
     2521 cggataaaca gcatggcctt ctggagcttg tctcggatgc ctatagtctg ggtctccgtc
     2581 cgctcaatcc ggacaacctc gaggtcctca tgtgcgattc tcgagtgagc tccggcgaag
     2641 cctcggtgtc ggtcgaggag gcggtggagg cgcaccacgc atcgatcgac atctgcgcaa
     2701 acatcgcgag cagcgcaagg cggtagcctt cccgtcaacc cttccaagtc ccccttgcca
     2761 tgatccgaaa attcggatac agttacccta tcgccttcat tgcacctgaa agcagaagtc
//
LOCUS       DASSLD010000226         5244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_6248,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000226 DASSLD010000000
VERSION     DASSLD010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6248"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..90)
                     /locus_tag="VFK23_04135"
     tRNA            complement(15..90)
                     /locus_tag="VFK23_04135"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55..57),aa:Ile,seq:cat)
     gene            complement(281..2107)
                     /gene="rpoD"
                     /locus_tag="VFK23_04140"
     CDS             complement(281..2107)
                     /gene="rpoD"
                     /locus_tag="VFK23_04140"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET7318309.1"
                     /translation="MAQKEHQSMSKKGKAGNSKKSGAAGTERNYVPFEEHGETIPHED
                     VSTEQLDSIISLFGDGDLDIDISDSRHDGKVRQPAEDELEDGVEPDEVGLDDAVEDEV
                     EEEDLEVGIGKTDDPVRLYLKEMGTVPLLSKEGEVAIAKRIEDGKREAAQVLYSLPFS
                     LQDVRDLGDKLRKGKISIHEVIHAGDDDFDDLQTQNEEELHARVLKLISEIDRHSKDL
                     TTLSAHVHNSRSTEARKKSLRDKMQTTRYALFDKLEALNIHCRQTDRLVLKLREMVSE
                     VLLSEREVHGLERKTALSLDELKDVFRKMRQGERELKNAARRLEIKQETLLVLEKTYR
                     NAAKRVKKIEEDCGVPAEDLKKALRAYERAEERAKIAKSELVEANLRLVVSIAKKYTN
                     RGLQFLDLIQEGNIGLMKAVDKFEWQRGYKFSTYATWWIRQAITRAIADQARTIRIPV
                     HMIETMNKLIRTSRGLVQDLGREPTTEEIAASMKLPVEKVRKIMKIAKEPISLETPIG
                     EEEDSHLGDFIEDKKVASPLESAIRKNLHQQVRKVLNTLTEREEKVLRWRFGIGEQTD
                     HTLEEVGQEFDVTRERIRQIEAKALRKLRHPSRSKQLKSFME"
     gene            complement(2158..3948)
                     /gene="dnaG"
                     /locus_tag="VFK23_04145"
     CDS             complement(2158..3948)
                     /gene="dnaG"
                     /locus_tag="VFK23_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET7318310.1"
                     /translation="MFSDDIINRVRDSVDIVDLVSGYVSLRKAGKNHVGLCPFHAEKT
                     PSFSVNPDKQIFHCFGCGVGGDVFKFLELQEGLNFPEAMKSLAGKAGIALPADTRARP
                     DRKAEDERAALLAVIAAAAGYFQQELEGPAGSAARAYLAKRGVSDQAVKDFRLGFARP
                     EWDGLLRHLRQKGHAPALMEKAGLVVKRSEGDGWYDRFRGRIIFPISDISGNVIAFGG
                     RVMDDSLPKYLNSPETPLYSKSNVLYCLDKAKDTARKLGYFIIVEGYLDALACHQFGA
                     RNAVATLGTALTEGHLRLMRRFAQNLVLIFDPDPAGVKAALRGLDLFVASGMKVNVVS
                     LPDGDDPDTFLNKNGYEAFASRLKGSEKFMDFVLGQVVRGGRNLSIDEKVEKAGEMLG
                     VIAKLPSGIERDYYLKKTAESLGVDEAVLRQEMPKQATRPHHAAAAASASAVGRGQRP
                     KAEEILIHLMLKDEAIARTLKGQLNSQDFSDPLFRRVAQRVFEVLDDAGRLDTRILLI
                     DGDEELNNIISHYSVLETTYHDSEQNCRDCVDVIKQRDPEKKMKFLCRAIQEAETRGD
                     TDEVARLMREQNELGKRPDRKLGKRVPGSK"
     gene            complement(3950..>5244)
                     /locus_tag="VFK23_04150"
     CDS             complement(3950..>5244)
                     /locus_tag="VFK23_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET7318311.1"
                     /translation="SLMAQSGLPVTAAEGSELPCFGSVFADIGDEQSIEQDLSTFSSH
                     AGHIAAILRQADGDSLVLLDELGAGTDPSEGAGLGAAVLESLIDRGSVTLVTTHHNAL
                     KLFGSEGRGAVNAAMEFDPATLLPTYRMIPGRPGRSYGLDMARKIGVPDEVIRKARAR
                     ISEDDTRLEDLLRQVEADTQRLTAEREAIEEELAASRRERAEAAVALSKARGEAREIK
                     ARAKAESREVVASLRQRLRELSRAAITGQTELRKQAAEVEALAAKLEPETFEQEQDAA
                     TAPELRPGDRVRIPRWNRSATVLASARGLLELDVDGKKLKLPHREVMPIEPLSGGQTA
                     YRGPGWSAELAEEEAPSDRLNIIGLRVSEGLAELERFIDRAGAVGLSMVTVIHGLGTG
                     ALKSAVMDYLKNHPQVAAIRPGEPAEGGAGVTVAELKK"
BASE COUNT          850 a         1737 c         1474 g         1183 t
ORIGIN      
        1 cccgccggtg aagttggtgg gcccaccagg actcgaacct gggaccaacc ggttatgagc
       61 cggtagctct accagctgag ctatgggccc tgctgagctt caaattccgg attgccggca
      121 ctgggccacg aggcgcaaaa cgcgaaaaat tttgtgcctc gttcctggag ccgtttggcg
      181 gtgtaatgct ggtgtacccg taaggtgccc gcctcgaaac ttggagcacc atattgtcac
      241 aacgcaccgg aaatgtcaag acattgccag cctgtgacgg ttactccatg aagctcttga
      301 gctgcttgct cctgctcgga tgacgcagtt tccggagcgc cttggcctcg atctggcgaa
      361 tgcgttcgcg cgtgacgtcg aattcctgtc cgacttcctc gagcgtgtgg tccgtctgct
      421 cgccgatacc gaaccgccac cggagcacct tttcctcgcg ctctgtcagc gtattcaata
      481 ccttccgcac ctgctgatgc aggttcttgc ggatggccga ctcaagcggc gacgcgacct
      541 tcttgtcctc gatgaaatcg cccaggtggc tgtcttcttc ctcgccgatc ggggtctcga
      601 gcgaaatggg ttccttggca atcttcatga tcttgcggac cttctcgacg ggcagcttca
      661 tggatgccgc aatctcctcg gtcgtcggct cccggccgag gtcctggaca aggccgcgcg
      721 acgtgcggat gagcttgttc atggtctcga tcatgtgcac aggaattcgg atggtgcgcg
      781 cctggtcggc gatggccctg gtgatcgcct gtcgtatcca ccaggtggcg tacgtgctga
      841 acttgtaccc gcgctgccac tcgaacttgt ccacggcctt catgaggccg atgttgcctt
      901 cctggatgag atcgaggaac tgaagcccgc ggttcgtgta cttcttcgcg atggaaacga
      961 cgagccggag gttcgcctcg accagctcgc tcttggcgat cttcgcccgt tcctccgccc
     1021 gttcgtaggc ccggagggcc ttcttgaggt cctcggccgg gacgccgcaa tcctcctcga
     1081 tctttttcac gcgtttggcc gcgttccggt aagtcttttc gagaacgagc agggtctcct
     1141 gctttatttc gagcctgcgc gccgcgttct tcagttcccg ctccccctgc ctcatttttc
     1201 tgaagacgtc cttcaactcg tccagggaaa gagcggtctt ccgttccaga ccgtggacct
     1261 cccgctccga cagcagcacc tcactcacca tttccctgag cttcaggacc aggcggtccg
     1321 tctggcgaca gtgaatattg agcgcctcaa gcttgtcgaa aagggcgtac cgcgtcgtct
     1381 gcatcttgtc ccggagcgat ttcttccgcg cctcggtgct ccggctgttg tgcacgtggg
     1441 ccgagagggt cgtgagatcc ttgctgtggc gatcgatctc gctgatcagt ttcagcacgc
     1501 gggcgtgcag ttcctcctcg ttctgtgtct ggaggtcgtc gaaatcatca tccccggcgt
     1561 gtatgacttc atggatgctg atcttcccct tcctcagttt atcgccgagg tcgcgcacgt
     1621 cctggagcga gaagggaagg ctgtagagga cctgcgccgc ctcccgcttc ccgtcctcga
     1681 tccttttcgc aatcgccacc tcgccttcct tggacaagag cggcacggtt cccatctcct
     1741 tgaggtagag gcggacggga tcatcggtct tgccgatgcc gacctcgagg tcttcctcct
     1801 cgacctcgtc ctcgaccgca tcgtcgaggc cgacctcgtc gggctcgacg ccgtcctcca
     1861 gttcgtcctc ggcgggctgg cgaaccttgc cgtcatgacg cgagtccgaa atgtcgatgt
     1921 caaggtcccc atcgccgaag agcgagatga tgctgtcgag ctgttccgtc gacacgtctt
     1981 cgtgcggaat agtttcgccg tgttcctcga acgggacgta gttccgctcc gtccctgccg
     2041 cgcctgattt tttcgaattg cctgccttgc ccttctttga catgctctgg tgctcctttt
     2101 gcgccacgtt atgtgcagcc gatgacgtct aatgatttag acctgttctc tttgctacta
     2161 cttactgccg ggaacgcgtt tgccgagctt tctgtccggt cttttgccaa gttcattctg
     2221 ttcacgcata agccgcgcaa cttcgtccgt gtcaccccgc gtctctgctt cctgaatcgc
     2281 ccggcacaga aacttcattt tcttttccgg gtctctctgc ttgattacat cgacgcagtc
     2341 ccggcaattt tgctcagaat cgtgataggt ggtctcaaga acggaatagt gtgaaattat
     2401 attattcaat tcctcatcgc catcaatgag caatatcctc gtatcaagcc ggcccgcatc
     2461 gtccaggacc tcaaagacgc gctgcgctac ccgccgaaac agcggatcgc tgaagtcctg
     2521 cgagtttaac tgccctttca gggttcgtgc aatcgcttcg tccttcagca tcaggtggat
     2581 caatatctct tcagccttcg gcctctgtcc gcggcctact gccgacgcgc tcgctgcagc
     2641 cgcagcatga tgcggccttg ttgcctgctt cggcatctcc tgcctcagga cggcctcgtc
     2701 aacgcccagc gactccgcgg tcttcttcag ataataatcc cgttcaatcc cgctcgggag
     2761 cttggctatg acccccagca tctcgccggc cttctccact ttctcgtcga tagagaggtt
     2821 gcggccgccc cgcacgacct ggccgagcac gaaatccatg aatttctccg accccttgag
     2881 gcgggatgcg aaggcctcat agccgttctt gttcaaaaat gtgtctggat catcgccgtc
     2941 gggaagggac acgacgttta ccttcatacc gctcgccacg aacagatcga ggccgcgaag
     3001 agccgccttc acgccggcag ggtcgggatc gaagatgagc acgaggttct gggcaaaccg
     3061 ccgcatgagc cggaggtgcc cctcggtcag ggccgttccc agcgtcgcga cggcgttcct
     3121 cgcgccgaac tggtggcatg caagtgcgtc aagatatccc tcgacaatga tgaagtatcc
     3181 cagtttccgg gccgtgtcct tggccttgtc caggcagtag aggacgttgc tcttgctgta
     3241 gagcggcgtc tcgggcgagt tcaggtactt gggcaaactg tcgtccatca ccctgccgcc
     3301 gaaggcgatc acgtttccgg agatgtccga aatcggaaag atgattctgc cccggaaacg
     3361 gtcgtaccag ccgtctccct cactccgttt gacgacgagc cccgctttct ccatgagagc
     3421 cggcgcatgg cccttctgtc tgagatgccg gagaagtcca tcccactcgg gcctggcaaa
     3481 gccaagtcgg aaatctttta ctgcctggtc gctgacgccg cgcttcgcaa ggtacgcccg
     3541 cgccgcgctt ccggccggcc cttcgagttc ctgctggaag tagccagcgg ctgcggcaat
     3601 gaccgcgagg agagcggcgc gctcgtcctc cgctttcctg tcgggcctgg ccctggtgtc
     3661 cgccggcagc gcgatccccg ccttccccgc cagactcttc atggcctcgg gaaagttcag
     3721 cccttcctgc agctccagaa acttgaacac gtctccgccc acaccgcacc cgaaacagtg
     3781 aaagatctgc ttgtccgggt tgacggaaaa cgacggtgtc ttttcagcat gaaagggaca
     3841 gagcccgacg tggttctttc ccgctttcct cagggaaaca taaccggaga cgagatcgac
     3901 gatatcaacg ctgtcccgga cccggtttat gatgtcatcg gagaacacgt tatttcttca
     3961 actccgccac cgtcacgccc gccccgccct cggcgggctc accgggccga atcgccgcga
     4021 cctgggggtg attcttcaga taatccatga ccgccgactt gagcgctccc gtgcccagac
     4081 cgtgaatgac cgtcaccatc gacaacccga cggctcccgc gcggtcgatg aaccgctcaa
     4141 gctcggcgag tccctcactc acccggagcc cgatgatgtt cagccggtcg gacggcgcct
     4201 cctcttcagc gagctcggcg ctccagcccg gcccccggta tgcagtctgc ccaccgctca
     4261 gcggctcgat cggcatgacc tcccgatgag gcagtttgag tttctttccg tccacgtcga
     4321 gttcgagcag accccgcgcc gaagcgagca ccgtagccga ccggttccac cgcggtatgc
     4381 gcacccggtc gccgggacgc agttcggggg ccgttgcggc gtcctgctcc tgctcgaagg
     4441 tctcgggctc gagttttgcg gcgagcgctt cgacttctgc ggcctgcttc ctgagctccg
     4501 tctgtccggt tatggccgcc cgggaaagct cgcggagcct ctgcctaagc gacgccacga
     4561 cctctcttga ttccgctttc gcccgggcct ttatctcccg agcctctccc cgcgctttgc
     4621 tgagcgccac tgccgcttcg gccctctccc tgcgtgacgc ggcaagctcc tcttcgatgg
     4681 cctcgcgctc tgccgtaagc ctctgcgtgt cggcctccac ctgcctcagc aggtcttcga
     4741 gcctggtgtc gtcctcgctg atgcgggccc gggcctttct gatcacttcg tccggcacgc
     4801 cgatcttacg cgccatgtcc aggccgtagc tcctgccggg cctgccgggt atcatccggt
     4861 atgtgggcag gagcgtcgcg ggatcgaact ccatggccgc attcacggcg cctcttccct
     4921 cggacccgaa gagcttgagc gcgttgtggt gcgtcgtgac aagggtcacc gaaccccggt
     4981 caatcaggct ctcgagcacc gctgctccga gtcctgcgcc ttctgacggg tcggtgccgg
     5041 ccccgagttc gtcgaggagc acgagcgaat ccccgtcggc ctgccggagg attgcagcaa
     5101 tgtggcccgc atgggacgag aacgtgctca agtcctgctc gatgctctgt tcgtcgccga
     5161 tgtcggcaaa gacggagccg aagcatggca gctcgcttcc ctccgcggcc gtcacgggca
     5221 agccggactg cgccatgagc gaca
//
LOCUS       DASSLD010000227         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_170950,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000227 DASSLD010000000
VERSION     DASSLD010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170950"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1412)
                     /locus_tag="VFK23_04155"
     CDS             complement(129..1412)
                     /locus_tag="VFK23_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7318312.1"
                     /translation="MNVARRVFEFLKLSSQAHARWDYEVSMSILKNRKKMLILLVALL
                     PILVIMFAEAGDMLGSKTAYAPAFYSTNIFLVSIAIGLCAGLITGCIGAGGGFIITPA
                     LMAAGVKGILAVGTDLFHIFAKAIMGTSVHKKLGNVSVKLAVAFLVGSAGGTFIGGAL
                     NKALYDKDPLLSEAFISLVYAVLLGFLGFYALADFLKSRKGKDTGDAHGGSSGLNELS
                     KKVQKINIPPAICFDEDFCQGGRKISGVIVALGGVVVGIMAAIMGVGGGFITFPMFVY
                     VFGVSSMTTVGTDILQIIFTAGLAAIGQYAIYGYVFYTLAMGMLLGSLIGIQVGALTT
                     KVVKGIHIRGFYAVSILAGFVNRVATLPKKMVELEMINLSQPVVKGIETFGNIVFWIV
                     VSLFAVWVIGKFIANLPTLRGEDERVKPALVKEEV"
     gene            complement(1442..>2022)
                     /locus_tag="VFK23_04160"
     CDS             complement(1442..>2022)
                     /locus_tag="VFK23_04160"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7318313.1"
                     /translation="ELVKNGYLKKQQGKGTFVTHVLQDLGVTMKTKLTEDMFGEGVKA
                     KKEVLAKGPKKPSEEMRSVLQLNGNGEEDNTLYTILCKRVVNGETAYLEESYVPLSVF
                     PGIEDEDVCRKPFYDLIQEKGVKKIFKVVQTIEVSEVRGEAAVLLKVKEGASGLLLHR
                     LLIGSDSSPIAYTRLLGMGKKYKLMTEFERIK"
BASE COUNT          478 a          560 c          517 g          467 t
ORIGIN      
        1 tttcgtcggc gaactcgagc ccgttcttcc cctggaacac aggcgagaag atcatgaata
       61 gcacaccgag aaaggtcaca gcgaacaatg caccgatatt gaacggcttt gaatttttga
      121 tcaacatgtt atacctcctc ctttacgagc gcgggcttca cacgctcatc ttcgccccga
      181 agcgtgggga gattagcgat gaattttccg ataacccata cggcgaatag ggagaccacg
      241 atccagaaga cgatatttcc gaaggtctcg atacctttga ccacgggctg ggagagattg
      301 atcatctcga gctccaccat cttcttcggg agagtcgcaa cccgattgac gaacccggcg
      361 aggatcgata cggcatagaa gccgcggata tgaatgcctt tcacaacctt cgtggtcaac
      421 gcgccaacct gaatgccgat cagagagccc aggagcatgc ccatggcaag ggtgtagaac
      481 acgtacccgt agatcgcgta ctgtccaatg gcagcgaggc cagccgtgaa gatgatctga
      541 agtatgtcgg tgcccaccgt ggtcatggag gaaacaccga agacatacac aaacatgggg
      601 aaggttatga acccgccgcc cacgcccata atggccgcca taatgccgac aaccacgcca
      661 cccaaggcca cgatcacgcc ggagatcttt ctacctccct ggcaaaagtc ctcatcaaag
      721 cagatcgccg gggggatatt gatcttctgt acttttttag aaagctcgtt caggcccgat
      781 gaaccgccat gcgcgtcgcc ggtgtccttg cctttacgtg acttcaggaa gtcagcaagg
      841 gcgtagaatc cgaggaagcc gagcagtacc gcgtaaacga ggctgataaa ggcctccgag
      901 agaagagggt ccttgtcata gagcgccttg ttcagtgcac cgccgatgaa agttcctccg
      961 gctgagccga cgaggaacgc gaccgcgagc tttaccgaaa cgttgccgag cttcttgtgg
     1021 acagatgttc ccataatcgc cttggcgaag atgtgaaaga ggtcagtgcc gacagcgagg
     1081 atgcccttca ctccggctgc catgagcgcc ggcgtgatga tgaacccgcc tcctgccccg
     1141 atgcatccag tgatcagtcc cgcgcagagg ccgatggcga tagagactag gaagatgttg
     1201 gttgagtaga aggctggtgc gtatgcggtt ttgctgccga gcatgtctcc ggcttcggcg
     1261 aacatgatga ccaggattgg cagaagcgcg acaagcaaaa tcaacatctt cttcctgttc
     1321 ttcaggatgc tcatcgacac ttcgtagtcc cagcgggcgt gggcttgtga tgatagcttc
     1381 agaaattcga aaacccttcg tgctacattc atggtaccct ccttattcat tggtatggat
     1441 tttatttaat acgttcgaac tcagtcatga gcttatattt tttccccata cccaaaagcc
     1501 gggtataggc gatggggctt gagtcagagc ctatgagcag cctgtgaagc aaaagcccgg
     1561 aagcgccctc cttgaccttg agcaatactg cggcttcacc cctgacctcc gacacttcga
     1621 tggtctgcac gaccttgaag atctttttta cccctttttc ctggatcagg tcatagaagg
     1681 gtttgcggca gacatcctcg tcctcgattc ctgggaatac agacagtgga acatacgact
     1741 cctcgaggta tgccgtctcg ccgttcacga cccgcttgca gaggatagtg tagagagtat
     1801 tgtcttcctc gccattccca ttcaactgga gaacggacct catctcctct gacggctttt
     1861 ttgggccttt tgcaagcacc tcttttttcg ccttcacccc ctccccgaac atgtcttcgg
     1921 tcaatttagt cttcatcgta acacccaggt cctgaagcac atgggtgaca aaggtccctt
     1981 taccctgctg ttttttgaga tatccgtttt taacaagctc gg
//
LOCUS       DASSLD010000228         2515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_725247,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000228 DASSLD010000000
VERSION     DASSLD010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725247"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..581)
                     /locus_tag="VFK23_04165"
     CDS             complement(156..581)
                     /locus_tag="VFK23_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HET7318314.1"
                     /translation="MIDINVSLLIQLVNFIVLLIALNFILFNPIRQIMQEREQGISSA
                     LEDAKAAQHRMQSLLEHYNSSLAESKQKATVTYNTIYQQGLDAQRDMISAERAKAGEM
                     LDKARAEIAAASNTARTDLQKEAERLAQDITSKLLGRAV"
     gene            complement(795..2216)
                     /gene="gltA"
                     /locus_tag="VFK23_04170"
     CDS             complement(795..2216)
                     /gene="gltA"
                     /locus_tag="VFK23_04170"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HET7318315.1"
                     /translation="MEAETKKAKRILQKKTPIPELDPRARRSNFDEVALGYTPELARQ
                     EAGRCLQCREPKCVTGCPVNVPIPWFIKAIHDGDFTRAIDIIKEVNLLPAVCGRVCPQ
                     ESQCEKLCTLSKKFEPVGIGRLERFAADREMMDGRAKVPVPEPRSGKRVAIIGSGPSG
                     LTCAYDLARRGHAVTIFEALHEPGGVLVYGIPEFRLPKKIVAREIEILKCMGVEIRTN
                     AVIGKLISIDEIIDEFDACYIGTGAGLPRFMGIEGENLNGVYSSNEFLTRINLMRAYR
                     FPEYDTPIKRGDRVAVVGGGNVAMDAARTALRLGATGVVMIYRRSLAELPARKEEVHH
                     AQEEGIRFELCTNPVRIIGEHGTVTAVECVRMDLCGLDESGRRSPKPVAGSEFHIPVD
                     IVIMAVGTGAHPLIPQSTKNLTVNKKKYIIADEETGRTSRDGVYAGGDIVTGSATVIS
                     AMGAGRKAARAMHEYLMQKESKP"
     gene            complement(2342..>2515)
                     /locus_tag="VFK23_04175"
     CDS             complement(2342..>2515)
                     /locus_tag="VFK23_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HET7318316.1"
                     /translation="CRVTVGGETLFACTDGPEFDGHLVDWDILMNRQKKYAPEQKESF
                     ESWHQRHRGALCE"
BASE COUNT          542 a          731 c          646 g          596 t
ORIGIN      
        1 gatgccaaaa ttgaccagcc tcggcagcca gtctcccaaa aaagtcatct cttctgcagg
       61 accttcttcc gcggccatgg caatgacgct ggcaaacatc accgcgagcg tatagatacc
      121 aagcatgatc tttttcatca ccaagctcct cacctctaca ccgcccttcc gagcagtttg
      181 gaggtaatat cctgcgccag ccgctctgct tctttctgaa ggtcggtgcg agccgtgttg
      241 gacgccgccg caatctcggc ccgcgccttg tccagcatct cgccggcttt tgcgcgctcc
      301 gcagagatca tatctcgctg tgcatcaagg ccctgctggt agatcgtatt ataggtgacg
      361 gtggccttct gcttggattc ggcgagcgag gaattgtagt gttccagcag gctctgcata
      421 cggtgctgcg cggccttggc atcttcaagc gcagagctga tgccctgctc gcgctcctgc
      481 atgatctgcc gtatgggatt gaagagaatg aaattaagtg caataagcaa tacgatgaaa
      541 ttgacgagtt ggataaggag tgaaacgtta atatctatca ttgtgaatcg cctcttttgc
      601 ggttaaagag aaacatctgt ttgttgcatg aaagtttaaa aaaactaaca taaccctaaa
      661 ccattgtcaa gatttttgcc tataaataat ataaatggct gcaaagtttt tgattataga
      721 aaaatcacgc gaaaaaacag gctgtcagag gcattcatgg tagaattgtt cggggaaaaa
      781 gaagagcagg aacgctaggg cttggactct ttctgcatca gatactcatg catcgctcga
      841 gcagccttcc tgcctgcgcc catggccgaa atgactgtag ccgagccggt tacgatgtcg
      901 ccgccagcat acactccatc cctgcttgta cggccggttt cttcgtcggc gataatgtat
      961 ttcttcttat tgacggtcag gttcttcgtg ctttggggaa tcagcggatg agcgccggtc
     1021 cctaccgcca tgatcacaat gtcgacaggg atatgaaatt ccgaacctgc caccggtttc
     1081 ggagatctcc tgcctgactc atcaagcccg cacaggtcca tccggacgca ctcgacggcc
     1141 gtgactgttc catgctctcc gattatccgg acgggattgg tgcagagttc gaagcggata
     1201 ccctcttcct gggcgtggtg cacctcttcc ttccgggccg gaagttccgc gagtgagcgg
     1261 cggtaaatca tgactacccc ggtcgcgccc agccggaggg ctgtccgggc ggcgtccatg
     1321 gcaacgttgc cgcctcccac caccgccacg cggtctcccc gcttgatggg agtatcatat
     1381 tcaggaaaac ggtaggcccg catgagattg atgcgggtaa ggaactcgtt cgatgaatag
     1441 acaccgttca ggttctcgcc ttcgatcccc atgaagcgcg gcagtcctgc ccccgtcccg
     1501 atatagcagg catcgaactc atcgatgatc tcatcgatgc tgatgagctt gcctatgacg
     1561 gcattggtcc tgatctccac acccatgcat ttcaggattt caatctcacg ggccacgatc
     1621 ttcttaggga gccgaaattc cggaatgccg tagaccagga ccccgcccgg ctcgtgaagg
     1681 gcctcgaaaa tcgtcaccgc atgcccgcgc cgtgccaagt cataagcaca ggtcagaccc
     1741 gaggggccgc tcccgattat agcgaccctt ttaccgctcc gcggctcagg gaccggcacc
     1801 ttcgcgcgcc cgtccatcat ctcccggtcg gccgcaaacc gttcgaggcg gccgatgccc
     1861 acaggctcga attttttcga cagggtgcag agcttttcac actggctctc ctgcgggcag
     1921 accctgccgc agaccgcggg caaaaggttg acctctttga tgatgtcaat agccctcgtg
     1981 aaatcgccgt catggatcgc cttgatgaac cagggaatcg ggacattcac cgggcagccc
     2041 gtgacacact tcggctccct gcactgaagg caccgccctg cctcctgcct tgccagctcg
     2101 ggtgtatagc ccaatgcgac ctcgtcaaaa ttagaccgtc tggcacgagg atccagttca
     2161 gggatgggcg tttttttctg caaaatcctt tttgcttttt tcgtttctgc ttccatatgc
     2221 gataagtatc tgttcactct gttagagaaa gccgctgact cctgtcgaag atgtacatcc
     2281 ctccctggag atggtgggct aaatatcagt cataatcgtc agggaccttt gtcatcggag
     2341 ttcactcgca gagcgcccct ctatgccgtt gatgccacga ctcgaacgat tctttttgct
     2401 cgggagcgta ctttttctgg cggttcatga gaatgtccca gtccacgaga tgaccgtcga
     2461 actcgggacc atcggtacag gcaaacaggg tctctccacc caccgtcacc cggca
//
LOCUS       DASSLD010000229         5223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1389064, whole genome shotgun sequence.
ACCESSION   DASSLD010000229 DASSLD010000000
VERSION     DASSLD010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389064"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VFK23_04180"
     CDS             <1..503
                     /locus_tag="VFK23_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318317.1"
                     /translation="LTEIARQNAIGTDYHLNEAPHQIVNTDHFKHQEFTLGITPEEYE
                     EVSDLLDWLIARQREGWTMVNSIVHLQTMKERMRGRLPRWDCRAGHNGALIRPDGTLS
                     PCFDLITYDHDWGRIWEPRFEREELNGVKETCLPNCSSTCYHTMASYYNIHTIPQWIL
                     KHARMG"
     gene            complement(510..1169)
                     /locus_tag="VFK23_04185"
     CDS             complement(510..1169)
                     /locus_tag="VFK23_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7318318.1"
                     /translation="MEPIPDPGVRLFCIPVLAVAFLLSSILLPAEAGAGRGWEITPYV
                     GYAIGGGFEDNSTGTKLDIRDGGNFGVVLDLSDTPETQYELFYGFQRTKVDSGGTFGG
                     DPLFDLDIHYLHLGGTYMFPRDKIRPYIAGGLGATHFVPNGSGLNPKTFFSLSLGGGA
                     KIPLSDHVGLRFEGRGFLTVLPDSTEIFCVSSGGAACRVSVKGDVFGQFLLLAGITFS
                     L"
     gene            complement(1265..1663)
                     /locus_tag="VFK23_04190"
     CDS             complement(1265..1663)
                     /locus_tag="VFK23_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318319.1"
                     /translation="MLNKRYFMLAAVTFALSLLAGGCAKEKSPPQPQPAKQEIARPEK
                     KAVPKPRAKLFTGTIEALDEEAGTLTLKGPKGTMRFHAHGKGKKQLDDLDIGDKVIVK
                     HIDMIPLSIVRPRTSKSVLASMTSPAIDPP"
     gene            complement(1769..1999)
                     /locus_tag="VFK23_04195"
     CDS             complement(1769..1999)
                     /locus_tag="VFK23_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318320.1"
                     /translation="MKKLLSVLFAAVFALTVSGFALAADNAAKAPKTETTVEKTEKKV
                     EKKKTTKKGKKSKKKTETKEEVTTETPAPVKK"
     gene            2406..4040
                     /locus_tag="VFK23_04200"
     CDS             2406..4040
                     /locus_tag="VFK23_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET7318321.1"
                     /translation="MAGSLPVAFLIAVCLCLPAQAQKVSPHSIPPLQKVAEKPTAESM
                     DDPLGRNTPFGTVMGFIKAVEGEDLNRSTEYLDTQQLPKRARMLAQELAAVLDAADLQ
                     DLSRKPQGNTEDDLPPNRERIGVVKTGSGSHEIFLDRTQRGKESPIWLFSADTLKWVP
                     RIHGELHVGWIERHISGTFLETRFLGQPLWALFGIILALPLTFAIARLATRLLLPVMQ
                     ASLRHVIPRAVDYPADRFQWPLCLLFMAVFFYALSLISFSAVSRVFWGYVAASVATIA
                     LTWISLRLIESAGGLFEGGPQSRLGSGRIAMARLLDKLSKALVIFFGALILFYIAGVN
                     LTAVLAGVGIGGIAIALAAQKSLENIFGAMMIVSDKAIRVGDFCRAGEYTGTVEDIGL
                     RSTRIRTLGRTVVSVPNGQLITMSLENFSERDKFLFHHRIHLGYETSAEQLRGCLSDI
                     RTMLREHTKAEMVTARVSLTAFLDSSIEIEIFAHLLEPSDEAFLTAQEGLLMKVMDIV
                     RANGAKFALPLPALSPAEGKDHKSINTKGFSSVSNG"
     gene            4319..4783
                     /gene="nrfH"
                     /locus_tag="VFK23_04205"
     CDS             4319..4783
                     /gene="nrfH"
                     /locus_tag="VFK23_04205"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HET7318322.1"
                     /translation="MRLAGRRKGLLLGVLAVSAIIAAALFLAVGPPKLLAKSDQPDFC
                     RQCHVMESNYEAWVHTGAHRRSKCVDCHLPNNNTGLHYVWKSIDGLKDVLFFYSGRVP
                     ERITLSAHGGTVLQANCVRCHETTVMMTDTDRKCWGCHRRVSHQRSGAMETL"
     gene            4799..>5223
                     /locus_tag="VFK23_04210"
     CDS             4799..>5223
                     /locus_tag="VFK23_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005984199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HET7318323.1"
                     /translation="MKVRQEFLCGLAAWLAAAALVLSGCQPPKTEVVRPVRIAEGEID
                     PAAWGKAYPVNYDLWKKTEEPTPAGKSKYKRGFNPDKITYDKLSEFPYMALLFNGWGF
                     GVEYNEPRGHAYMMKDQINIDPSRVKAGGVCLTCKTPYAP"
BASE COUNT         1091 a         1590 c         1488 g         1054 t
ORIGIN      
        1 tgctcacgga gatcgcccgc cagaatgcga tcggaacgga ctatcacctc aacgaggccc
       61 cgcatcagat cgtcaatacc gaccacttca agcaccagga gttcaccctg ggcatcaccc
      121 cggaggaata tgaggaggtg agtgatctcc tggactggct cattgccagg cagagggaag
      181 ggtggaccat ggtcaattcc atcgtgcacc tgcagaccat gaaggagagg atgcgtggcc
      241 gtctcccccg ctgggactgt cgcgccggac ataacggcgc cctgatacgc cccgacggaa
      301 cactctcccc gtgtttcgac ctgatcacct atgaccacga ttggggaagg atctgggaac
      361 cgagattcga acgggaagag ctcaatgggg ttaaggagac ctgcctgccg aattgctcct
      421 ccacctgcta tcacacgatg gcttcctatt acaacataca caccatcccc cagtggatcc
      481 tgaagcacgc ccggatgggc tgacccgcct cacaacgaaa aggtgatccc cgccaacagc
      541 aggaactggc cgaacacgtc ccccttgacg ctcacgcgac aagcagcgcc gccggacgag
      601 acgcaaaaaa tctccgtgga gtccggcagg accgtcagaa accccctccc ctcgaagcga
      661 aggccgacgt gatcggatag cggtatcttg gctcccccgc ccagcgagag ggagaagaac
      721 gttttcgggt tcagcccgct cccgtttggt acgaaatgag ttgcgccgag tcctccggca
      781 atgtaaggcc ggatcttatc ccggggaaac atgtaggtcc cccccaggtg gaggtagtgg
      841 atgtccaggt cgaaaagggg atccccgccg aacgtacccc cgctgtcgac tttcgtccgc
      901 tggaatccgt agaagagttc gtactgggtt tccggggtat ccgacaagtc gaggacgacc
      961 ccgaagttcc ccccatccct gatatccagc ttggtgccgg tactgttgtc ctcaaaacct
     1021 ccgccgatgg cgtagccgac gtacggcgtg atctcccaac cccgtcctgc tcctgcctcc
     1081 gcgggaagca gaatcgatga aaggaggaac gcgaccgcca gcacgggaat gcagaacagg
     1141 cgcacaccgg ggtcaggaat cggctccatg gtttcctccg ggaacctccc tcccccggcc
     1201 caggcgccgt cgaaggttcc ccgcccgggg gtgcaggaac gtcccgatga ttaccccacc
     1261 aagatcaggg tggatcgatc gcgggagaag tcatgctggc caagacactt ttgctcgtgc
     1321 gaggcctcac gatagagagg gggatcatgt cgatgtgctt cacgattacc ttgtccccga
     1381 tatccaagtc atccagctgc ttctttccct tcccgtgggc atgaaaacgc atcgtcccct
     1441 tgggtccctt cagagtcagg gtccctgcct cctcgtccaa ggcctcgatc gtccccgtaa
     1501 ataatttcgc gcggggcttc gggacagctt tcttttccgg tctcgcaatc tcctgcttcg
     1561 cgggttgggg ctggggaggc gatttttcct tggcgcaccc acccgctagg agagagaggg
     1621 caaacgtcac cgctgcgagc atgaaatacc gtttattcaa catattcctc aaggtgaaat
     1681 aaaaaaaggg cagggagatt gtgtccccct gcccgtggcg tcctcgtcct tgtggggcgg
     1741 gacggtcctt ccgttgtgac cgcttggact acttcttcac cggagcggga gtttctgtcg
     1801 tcacttcttc cttcgtctcg gtcttcttct tcgacttctt ccccttcttc gtcgtcttct
     1861 tcttctccac cttcttctcc gtcttctcta ccgtcgtctc cgtcttcggc gccttcgcag
     1921 cgttgtcggc tgcgagcgcg aaaccggaca cggtcagggc gaaaacagcg gcgaacagaa
     1981 ccgaaagcag tttcttcatt tctttccctc catgggagat ttgaaatctg ggaaaggtta
     2041 atacaaggac agtgccaatc cagcagattg tgaaactatt taaaaagatg tgtctttttc
     2101 cttcaacccg aaatcgtctc cagcggtgcg ttccccaaaa gaggcactgc atccccattt
     2161 gggggaacac cgttcccgca agaccccaat ttcacccgga ccggattgaa taagtctggt
     2221 cctggggcac ccatcctcat cgggaatcat ctaaaggtca cgtcgtacca aaagaggtgc
     2281 tccatggatc caaaccgtgc cacgtcggta ccccgatgaa gcagagggcc gtcatccggt
     2341 aacccatgct ctcgatccgg aatctcttcc gtaggaaatc gccggccctg cgagcagggg
     2401 gaatcttggc cggctcgctc ccggtggcat tcctcatcgc agtgtgcctg tgccttccgg
     2461 ctcaggcaca gaaggtatcg cctcattcga tccctcctct tcagaaggta gcggaaaaac
     2521 cgactgctga gtcgatggac gaccctttgg ggagaaacac cccctttgga acggtcatgg
     2581 gattcataaa agccgtggaa ggcgaggact tgaaccgctc cacggagtac ctggacacac
     2641 agcagctccc gaaacgggcc cgaatgttgg cccaggaact tgcagccgtc cttgacgcgg
     2701 cagatcttca ggatctgagc aggaagcctc aagggaacac ggaggacgac ctgccgccca
     2761 accgggagcg catcggcgtg gtgaagacag ggtcgggctc ccacgaaatc tttcttgacc
     2821 ggacgcaacg ggggaaggag tctccgatct ggttgttctc cgccgacacc ctgaagtggg
     2881 tgccccggat ccatggcgaa ctccatgtcg gctggatcga gcggcacatc tcggggacgt
     2941 ttctcgagac ccggttcctc gggcagccgc tctgggcctt gttcggcatc atcctggccc
     3001 tgccgttgac cttcgccatt gcccggctgg ccacccgtct gctcctgccc gtcatgcaag
     3061 cgtcgcttcg ccatgtcatc ccccgggccg tcgactaccc cgcggacagg ttccagtggc
     3121 ccctatgcct gctgttcatg gcggtgttct tttacgccct atcgctcatc tccttttcgg
     3181 cagtcagccg ggtcttctgg gggtatgtag cagcgagcgt cgcgacgatc gcgcttacct
     3241 ggatcagctt gcgcctgatc gaaagcgcag gaggattatt tgaaggggga ccccagtcgc
     3301 gcctcggatc aggcagaatc gccatggcac gtctcctgga caagctgtcc aaggcgctcg
     3361 tgatattctt cggcgcgttg atcctcttct atatcgccgg ggtcaacctt acggccgtcc
     3421 ttgccggagt gggcatcggc ggcatcgcca tcgcccttgc ggcgcagaaa tcgctggaaa
     3481 acatcttcgg cgcgatgatg atcgtctccg acaaagcgat tcgcgtgggc gatttttgcc
     3541 gggcgggcga atacacgggc accgtggagg acattggcct gcgctcgacg cgcatcagga
     3601 cgctcggtag gaccgtcgtt tccgttccga acggtcaact catcaccatg agcctcgaga
     3661 acttctccga gagggacaag ttcctcttcc atcaccggat tcatctggga tacgaaacgt
     3721 ccgcagaaca gctgcgcgga tgcctctcgg atattcgaac catgctccgc gagcacacaa
     3781 aggccgaaat ggtgaccgcc cgggtgagcc tcacggcatt tctcgactcg tccatcgaga
     3841 tcgagatctt cgcccatctc ctagaaccgt cggacgaggc atttctgacc gcccaggaag
     3901 ggctccttat gaaggtcatg gatatcgtca gagccaacgg cgcaaaattc gcgctcccgt
     3961 tgcccgcact ctctccggcg gaaggaaagg accataaatc cataaacaca aaagggtttt
     4021 cttccgtttc gaacgggtaa ggttctgacg cattgtaaac ccccgtggca acctgctacg
     4081 gagggagtcg aacctcgcgc gccgggcgat ccgctataaa ccacatcaat cggggcggta
     4141 tctccgatca catctctttt cccccgcgtc cgaaaggaca tggggcgaac cggtcaagac
     4201 ttttcttcac cccggagact ttcataatgt atactgtata caatttaagg tgacgcaagc
     4261 gacgggacct ggcaccggtc gatcctgccc cccggggaac gggagaggag ggatgaagat
     4321 gaggctggcc ggaagaagga agggtcttct ccttggcgtt ctcgcggttt ccgcaatcat
     4381 cgctgccgct ctcttcctgg cggtgggccc gccgaagctg ctggcaaaga gcgaccagcc
     4441 ggatttctgc aggcagtgcc acgtcatgga atccaactac gaggcgtggg tccacaccgg
     4501 tgcgcataga agaagcaaat gcgtcgactg tcatcttccc aacaataaca ccgggcttca
     4561 ctatgtctgg aagtcgatcg acggactgaa agacgtgctc tttttctatt cggggagggt
     4621 gcccgagcgg atcacgctgt cagcccacgg agggaccgtc ctgcaggcga attgcgtgcg
     4681 ttgccatgag accaccgtca tgatgaccga tacggatcgg aaatgctggg gctgccacag
     4741 gagagtctcg catcagcgca gcggcgcgat ggaaacgtta taaacaagga ggaccgggat
     4801 gaaggtgcga caggaatttc tgtgcggact ggccgcctgg cttgcggcgg cggccctggt
     4861 tctttccggc tgccagccgc cgaagacgga ggttgtgagg cccgtgagga tcgccgaggg
     4921 ggaaatcgac cccgcggcgt ggggaaaggc gtaccccgtc aactacgatt tgtggaagaa
     4981 gaccgaggaa ccgacgcccg ccgggaaaag caaatacaaa agagggttca acccggacaa
     5041 gatcacctac gacaagctgt cggaatttcc ttacatggct ctcctgttca acggttgggg
     5101 gttcggggtc gaatacaacg aaccgcgggg ccacgcctac atgatgaagg atcagatcaa
     5161 tatcgatccc tcgagagtga aggccggcgg cgtctgcctc acgtgcaaga ctccttatgc
     5221 ccc
//
LOCUS       DASSLD010000230         3870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_472455,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000230 DASSLD010000000
VERSION     DASSLD010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472455"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VFK23_04215"
     CDS             <1..1011
                     /locus_tag="VFK23_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7318324.1"
                     /translation="GADNGACEHLVKAENASLRARDLTRQLLTFSRGGAPVRKKASLR
                     EVIKESAGFALRGSDVTCEFSIPDDLWPVEMDEGQIGQVVRNLVINANHAMPDGGTIR
                     ITCRNVRVPEGVLPLKKADYVRVSVEDRGTGIAKEHLPKIFDPYFTTKPKGSGLGLAA
                     SYSIIKNHDGYITVESEPGVGTTFHFYLPASDTRGRAAPARVDEQPSAGGGRILVMDD
                     EEIVRDVVADMLKHLGYEVEVARDGVEMLIRYMEARNSDKTFDAVIMDLTVPGGMGGK
                     DAVRKLLEVDPAVKAIVSSGYADNQIMADYKNYGFAGVIAKPFVINHLAATLDSVIKP
                     SA"
     gene            complement(1062..1799)
                     /locus_tag="VFK23_04220"
     CDS             complement(1062..1799)
                     /locus_tag="VFK23_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET7318325.1"
                     /translation="MRTAMLSDIHANLEALTAVLDRLKSLKVDDIVCLGDTVGYNANP
                     NEVLDILRKEKAQCIIGNHDACAAGLEEPEDFNPLARAAVLWTREKLTEENRQFLAGL
                     PRERRIRDFFIFHGSIHDTDRYILYRNDAKDNFELLAGLPGPVSVGFFGHTHVRVSLI
                     EQHGIISAALAIEDLDLVPGKRYLINPGSVGQPRDGDPRPAFAVYDDRDRRVTFHRVE
                     YDIRACQDKIISAGLPPRLAERLQWGM"
     gene            2049..2774
                     /locus_tag="VFK23_04225"
     CDS             2049..2774
                     /locus_tag="VFK23_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET7318326.1"
                     /translation="MHAKIKIGCCGFPVSRERYFRAFEVVEIQQTFYQPPEDRLAAKW
                     RAEAPEAFEYTLKAWQLITHEPSSPTYRKLKAPIAGSKEKFYGSFKPTAEVFSAWERT
                     RTIAELLRARVIVFQCPPSFIPSAEHKKNMRTFFSSVRRDGFVFAWEPRGTWMPDEIR
                     NLCEELDLVSIVDPLKAAPQYGTIRYFRLHGKGGYRYKYTDGDLAALKRATETMQETY
                     VLFNNIFMYDDAVAFKELRSRGR"
     gene            2930..>3870
                     /locus_tag="VFK23_04230"
     CDS             2930..>3870
                     /locus_tag="VFK23_04230"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET7318327.1"
                     /translation="MEDYDNLLAELAELAGILPEYYDIFGNRHVASRETKVAILSELG
                     FPAGTREDIIRGIEKKTLRLWRAVLDPVTVISVRDQPYRLAVHLPMPEGKEQATGIVL
                     TVEDEEGKKQTIDFPAGTLKAEDQRMIDNRRFVRILLSIPSREIGYYTLSVACSHPEP
                     VFGNGACPLERSARLIIAPDSCYMPEQLRTGRTWGIALNLYALRSERNWGVGDLGDLR
                     RLVVWASQLEAGLVGINPLHAIPNTFPFGISPYSPVSRLYRNLIYIDMDSVADLDRIP
                     GSKQTAEKIATLRKSERIDYEGVAAVKQEMLEQAYEL"
BASE COUNT          854 a         1084 c         1154 g          778 t
ORIGIN      
        1 ggcgccgaca atggcgcatg cgaacacctg gtcaaggcgg aaaacgcttc tctccgggcg
       61 agggatctga cccggcagct tctcaccttt tcccggggag gggccccggt gagaaaaaag
      121 gcctctctca gggaggtcat aaaggagtcg gccggctttg ccctgagggg ctccgatgtc
      181 acgtgcgagt tttccattcc cgacgacctg tggccggtgg aaatggacga gggtcagatc
      241 ggccaggtcg tccggaatct ggtcatcaat gccaaccacg ccatgccgga cgggggaacg
      301 atcaggataa cctgcaggaa cgtccgcgtt ccggaagggg ttctgccgct gaagaaggcg
      361 gattatgtcc gggtgtccgt cgaagaccgg gggacgggaa tagcgaagga acatcttccg
      421 aaaatattcg acccctattt tacgacaaag ccgaaaggca gcggcctcgg gctcgccgcc
      481 agttattcca tcatcaagaa ccacgacgga tatattacgg tggaatcgga accgggggtg
      541 ggaacgacgt ttcattttta tctgcccgct tcagacacga ggggccgcgc ggccccggcc
      601 cgcgtcgacg aacagccttc cgccggaggg gggaggatcc tcgtgatgga cgatgaagag
      661 atcgtgaggg acgtcgtcgc cgacatgctg aaacatctgg gatatgaggt tgaggttgcc
      721 agggacgggg tcgaaatgtt gatccggtac atggaggcca ggaattcgga caagacgttt
      781 gacgcggtca tcatggattt aacggtcccg ggaggcatgg gcggcaaaga tgccgtcagg
      841 aaactcctgg aagtcgaccc cgcggtgaaa gcgatcgtat cgagcggtta cgcggacaat
      901 cagatcatgg ctgattataa aaactacgga tttgccggcg tgatagcaaa acccttcgtg
      961 atcaatcacc tggccgcgac actggattca gtaataaaac cttccgcctg aacgcccctt
     1021 ccttgagtcc gtgatcgtcc gcggaaaacc gggcacttct ctcacattcc ccattgaagc
     1081 cgctccgcga gccgcggcgg caggcccgcc gagatgatct tgtcctggca cgcgcgtata
     1141 tcgtactcca cgcggtggaa cgtcaccctt cggtcccggt cgtcatagac ggcaaaggcc
     1201 gggcgagggt ccccgtcgcg gggctggccc acgcttcccg ggttgatcag ataccgcttt
     1261 cccggaacca ggtccaggtc ctcgatggcg agcgccgccg agattatgcc gtgctgttct
     1321 atcagagaca ccctcacgtg cgtatggccg aagaacccga ctgacaccgg ccccggaagg
     1381 ccggcgagga gctcgaaatt gtccttggcg tcgttccggt aaaggatgta gcggtccgtg
     1441 tcgtggatgg atccgtggaa aatgaaaaag tcgcgaattc ggcgctcgcg cggcaggccc
     1501 gcgaggaatt gcctgttttc ttcggtgagc ttttcccgtg tccagaggac ggcggcccgg
     1561 gccagggggt tgaagtcttc gggctcttcg agaccggcgg cgcaggcgtc atgattgccg
     1621 attatgcact gggccttctc cttccggagt atgtccagga cctcgttggg gtttgcgttg
     1681 tatcccaccg tgtccccgag gcagacaatg tcatccacct tgagcgattt cagccggtcc
     1741 aggaccgcag tcagcgcttc aaggttcgcg tgaatatcgg agagcatcgc ggtacgcatg
     1801 tcgcatccac ctctcaagca actcttttcc tgactaaatt ctagcaaatc cagccgcaag
     1861 tgcataaaca tggaaagggc agttggacgc tgatgtgcgc agattttcgc ggataaaccg
     1921 ggaaacttcg agaggatatc tactcgatat ccgggtgatt tctttagtca acgagtcttc
     1981 gctcgtctta cgaaagatcg aaaaaatttc cggtacaata gcaataaaga gtcggttact
     2041 ggaccatcat gcatgcgaag atcaaaatcg gctgctgcgg cttccctgtt tcgcgggagc
     2101 gctacttcag ggctttcgag gtcgtggaga tacagcagac cttctaccag ccgccggagg
     2161 accgccttgc ggcaaaatgg cgcgccgaag ccccggaggc gttcgagtac acgctcaagg
     2221 catggcagct cataacccat gaaccttcaa gtccgacgta ccggaagctc aaggccccga
     2281 tagcgggttc gaaggagaaa ttctacggat cgttcaaacc gacggccgag gtcttttccg
     2341 cgtgggagag gactcgcacg atagccgagc tgcttcgcgc gcgggtcatc gtcttccagt
     2401 gtccaccgag cttcattccg tccgcggagc ataaaaagaa catgaggaca tttttttcat
     2461 cggtcaggag ggatgggttc gtttttgcct gggaaccgcg gggaacatgg atgcctgatg
     2521 aaatacgaaa tctttgcgag gaactggatc tcgtatctat tgtggacccg ctcaaggccg
     2581 cgccgcagta cggaacgatc cgctacttcc ggctccacgg caagggcggc taccggtaca
     2641 aatatactga tggagattta gcagcgctca aacgcgcgac ggagaccatg caggaaacct
     2701 acgtactgtt caacaacata ttcatgtatg acgacgcagt tgcgttcaag gagttgcgga
     2761 gccgggggcg ctgacggttc cctcccctat tcgaggtatg ccggtcccat catcgcatag
     2821 gtgcccgaaa tcattcacgt gggcaactct ccccgaaacc ctccccggta aacccatcga
     2881 ccgcggcacg aaaagaaatc tctcgggcaa aacgtggtaa tatctgagca tggaagatta
     2941 cgataatctc ctggccgagc tggccgagct tgccgggatt ctccccgagt actacgatat
     3001 cttcggcaac aggcatgtcg cttcccggga aacaaaggtc gcgatcctgt cggaactggg
     3061 cttcccggcc gggacccggg aagacatcat ccggggaatc gagaagaaaa cattgcggct
     3121 ctggcgcgcc gtgctggatc ccgtaacggt catctctgtt cgagaccagc cgtaccgcct
     3181 ggccgtgcac ctgccgatgc cggaaggaaa ggagcaggca accgggatcg tactcaccgt
     3241 tgaggacgaa gaggggaaga agcaaacgat cgattttccg gccggcacgc tcaaggctga
     3301 agaccagcgg atgatcgata accggcggtt cgtaagaatc ctgctttcga ttccctccag
     3361 ggaaatcggg tattacaccc tttccgttgc ctgctcgcac cctgagccgg tgtttggcaa
     3421 tggcgcctgc ccgctcgaac gatcggcccg tctgatcatt gcgccggatt cgtgctatat
     3481 gccggaacag ctccggacag gcagaacctg gggtattgcg ctcaatctct atgccctgcg
     3541 ttcagaacgg aactgggggg tcggcgatct gggcgacctc cggaggctgg tcgtctgggc
     3601 gtcacagctg gaagccggtc tggtcgggat caacccgctt catgccattc ccaatacgtt
     3661 tcccttcggg ataagcccct attctcccgt cagcaggttg tacagaaacc tcatctatat
     3721 cgacatggac agcgttgccg atctggatcg tattcccggg tcgaagcaga ccgctgaaaa
     3781 aatcgcgacc ctcagaaaaa gcgagcgcat cgactatgag ggcgtagcgg ccgttaaaca
     3841 ggagatgctt gaacaggcct atgaactgtt
//
LOCUS       DASSLD010000231         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_659498,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000231 DASSLD010000000
VERSION     DASSLD010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659498"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1728)
                     /gene="pheT"
                     /locus_tag="VFK23_04235"
     CDS             complement(10..1728)
                     /gene="pheT"
                     /locus_tag="VFK23_04235"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET7318328.1"
                     /translation="MPTIDIKKRDLDGLIGRKLSLNVLEKKLMLAKAELKEYNAETDE
                     LKVELSDSNRPDLWSAEGVARQIRIFLSGKPESYSFFDRGQKATRVITVAKDMKSVRP
                     YIAACTASGIRMNDDVLAQMIQSQDKLAEIFGRKRATVSIGIYELDRIAFPVSYGLAD
                     PGSVSFTPLGMEEKLTLAEILERHPKGIAYGGIVKPFSRYPLLTDSMGRVLSFPPIIN
                     SREIGEVKPQTRNVLVEVTGTDLRMTALTLNILAASFYDRGAEVQPVLVSYPYDTEFG
                     RRVSFPVDTTGKFNVPLATFSAGLGEDVSAADAKKYLSLYGHSVSAKKGVVTVSVPPY
                     RDDIMHPMDLVEDFAISRGYESFEPVMPRQSTVGSLSLLELLSDNVRGIMVGAGFQEI
                     MSNILCSREELAYAMPEGEKLVEVDNVMSLAYSVLRNRVVPSLLSVEAASSKAFYPHR
                     IFEAGEVARFADDENMGSRTELNLGALIAHPGANFSEMHSTLDILLYYLGVEYRLAPA
                     EHPLYMPGRCGSIAAAGVNLGFIGEVRPGVLERTQITMPCAAFEITLNAILELGNSRS
                     QRTQTG"
     gene            complement(1847..>2669)
                     /locus_tag="VFK23_04240"
     CDS             complement(1847..>2669)
                     /locus_tag="VFK23_04240"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET7318329.1"
                     /translation="NPPRQGIGRKHPYKEFLDFVKYKFIGMGFEEMRGPLVENEFWDM
                     DALFMPQFHPARNIHDVYFVKEPAASREVEQPFARRVAAAHKDGGKTGSTGWRYQFDL
                     ERTKRLILRSQGTAVSARTLASGPKIPGKYFSIARCFRYDAVDATHAPDFYQVEGIVL
                     GEDINFTALLGLLKLFGREIARAGEFQFRPAYFPFTEPSVELHVKHPELGWMELGGAG
                     IFRPEVTKPLGVDVPVIAWGLGLDRMAMVALGIQDIRDLFSRDLDFIRTKKIKLD"
BASE COUNT          521 a          813 c          791 g          544 t
ORIGIN      
        1 agttgaagat tatccggttt gtgtcctctg cgacctggaa tttcccagct ccaggatggc
       61 gttcaaggtg atctcgaagg ctgcgcaggg catggtgatc tgcgttcgtt cgagcacccc
      121 gggccggacc tcgccgatga agccgaggtt cacgccggct gcggcgatcg acccgcagcg
      181 gcccggcatg tagagcggat gctcggcggg tgccagccgg tactccaccc ccagatagta
      241 gagcaggatg tcgagcgtcg aatgcatttc cgaaaaattc gcgccgggat gggcgatgag
      301 cgcgccgagg ttcagttccg tgcgcgaccc catgttttca tcgtccgcga agcgcgcgac
      361 ctctccggcc tcgaagatcc ggtggggata gaaggccttc gagcttgccg cctcaacgga
      421 gaggagcgac gggacaaccc ggttccggag cacggagtag gcgagcgaca tgacattgtc
      481 cacttcgacc agtttctccc cctccggcat ggcataggcg agttcttccc gggagcagag
      541 gatgttggac atgatctcct ggaagccggc gccgaccatg attccgcgga cgttgtccga
      601 gagcagttcg aggagtgaaa gcgaacccac cgtcgactgc ctcggcatga ccggctcgaa
      661 cgattcatat cccctgctga tcgcgaaatc ctcgaccagg tccatcggat gcatgatatc
      721 gtcccggtag ggaggcacgg agacggtgac cactcccttc ttcgccgaga ccgaatggcc
      781 gtacaaactc agatatttct tcgcgtccgc ggcggaaaca tcctccccca gcccggccga
      841 gaacgtcgca agcggcacgt tgaattttcc cgtcgtatca acagggaatg aaacgcgcct
      901 tccgaattcc gtgtcgtagg gataggacac gagcacgggc tggacctcgg cgccccggtc
      961 gtaaaagctc gcggcgagga tgttcaaggt gagcgccgtc atccgcaggt ccgttcccgt
     1021 cacctcgacg agcacgttcc tcgtctgcgg cttcacctct ccgatctcgc ggctgttgat
     1081 gatcggcgga aacgagagca cccggcccat gctgtccgtc aggaggggat accgggaaaa
     1141 gggttttaca atgccgccgt aggcgatgcc cttgggatgg cgttcgagga tctcggcgag
     1201 cgtgagcttc tcctccatcc cgaggggcgt gaacgacacc gatcccgggt ccgccagccc
     1261 gtacgagacg ggaaacgcga tgcggtcgag ctcgtagatg ccgatcgaca ccgtggccct
     1321 cttccttccg aagatctcgg cgagtttgtc ctggctctgg atcatctgcg ccaggacgtc
     1381 gtcgttcatg cgtatgccgc tcgcggtgca tgcggcgatg taggggcgca cggacttcat
     1441 gtcctttgcc acggtgatca cccgcgtggc tttttgtccg cgatcgaaga acgaatacga
     1501 ttccggcttt ccggacagga agatccgtat ctggcgcgca acgccctcag ccgaccagag
     1561 gtccggacgg ttgctgtccg aaagctccac cttgagttcg tcggtctcgg cgttgtactc
     1621 cttgagttcc gccttcgcga gcatcagctt tttctcaagg acgttcaggg aaagctttcg
     1681 accgatgagg ccgtccaggt cgcgtttttt gatatctata gtaggcatac gttaataaca
     1741 ccactgatgt catacctgcg aaagcaggta tccattagct attctaagac aacatcaaca
     1801 tggattcccg ctttcgcggg aatgacaaat aatatagatg tcgtggtcaa tcgagtttta
     1861 tttttttcgt cctgataaaa tccaaatccc ttgaaaacaa atcgcggata tcctggatcc
     1921 ccagggccac catggccatg cggtcgaggc cgaggcccca ggcgatgacc ggcacgtcga
     1981 cgccgagggg cttggtgacc tcgggtcgga atatccccgc accgccgagt tccatccatc
     2041 ccagttccgg gtgctttaca tgaagttcca ccgagggctc ggtgaaggga aaatacgcgg
     2101 gccgaaactg gaattccccc gcccgggcga tctcccggcc gaacagcttc aaaagcccga
     2161 gcagcgcggt gaagttgatg tcctcgccga gaacgatgcc ctcgacctgg tagaaatcgg
     2221 gcgcgtgcgt ggcgtcgacc gcgtcgtagc ggaaacagcg ggcgatcgaa aaatattttc
     2281 ccggtatctt gggtcccgac gccagggtcc gggccgacac tgccgtgccc tggctccgga
     2341 gaatcaggcg ttttgtccgc tccaggtcga actgataccg ccagcccgtg gaacccgtct
     2401 tccctccgtc cttgtgcgcc gccgcgaccc ttctcgcgaa gggctgctcg acctcccggg
     2461 atgcggcagg ctccttcacg aagtacacgt catggatgtt tctggccgga tggaactgcg
     2521 gcatgaacag cgcgtccatg tcccagaact cgttctcgac gagcggcccc cgcatttcct
     2581 cgaaccccat gccgatgaac ttgtacttca cgaagtcgag aaactcctta tagggatgct
     2641 tcctgccgat gccctggcgc ggcggattg
//
LOCUS       DASSLD010000232         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_560580,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000232 DASSLD010000000
VERSION     DASSLD010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560580"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VFK23_04245"
     CDS             <1..355
                     /locus_tag="VFK23_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HET7318330.1"
                     /translation="QDLFSLSRNHYDRIGHFMQGFVPALLAREILLRGGFVTNRRMAA
                     FLAVCIAMAISAWYELIEWAAAVILGQGADAFLGTQGDPWDTQEDMFMCFIGAIVALL
                     TLSREQDRQMANFTR"
     gene            453..632
                     /locus_tag="VFK23_04250"
     CDS             453..632
                     /locus_tag="VFK23_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318331.1"
                     /translation="MVVDLEKKEVDLLVNELEHATIPNLREFIASGLREEHREELKQD
                     EVVLKHLLEKLKMAA"
     gene            743..991
                     /locus_tag="VFK23_04255"
     CDS             743..991
                     /locus_tag="VFK23_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318332.1"
                     /translation="MVDIKLEVTIYAGYRGSERPKSFLFEGNTVEVAGIRQMWVEEEH
                     KTRMQKRFFMLTGNDAYEYTLYHELESGEWFLRKREKK"
     gene            complement(1020..>2549)
                     /locus_tag="VFK23_04260"
     CDS             complement(1020..>2549)
                     /locus_tag="VFK23_04260"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HET7318333.1"
                     /translation="PVRAGKLNHETAFVLEKFGVPPSAEIDSLVATVSDLDLKRPIAV
                     GVRDSIQTLALMMREQGVRSVPVVDESGRLAGVVGLKDIARHYIDSVGFADLSKAPIE
                     LDILLTTLDGRVISNTKQISVLTGRVLIAAMQRGTMLNRVSPGDVVVAGDQNDVHLEL
                     IRVGCSALIVTDGAPIANEVVAAARQKDVLLISSRHPAFATVQLMTMSEPVSSIMTTA
                     CPTVGLYTPMAEVRSKIVESDYRSVVVVDSDNRLIGFITRTDLLKPVRKIAVLVDHNE
                     LSQAVDGIEEAEILEIIDHHRVGDISTTAPIYVYNDPVGSTCTVVAGIMFLYQTHIPK
                     EIAGLLLSGVLSDTLLLTLSTTTERDRQEARRLAEIAGVDIQSYARELLHASINLEDK
                     TAAELIAADFKEFLIGGKRLGVSQMMSLDCAEIDARERELVAELERLRAANNYDLTVL
                     LVLNPLGKVQERILLRGEAWIVEKAFGVKVVNDTCTVPRVMSRKKDFIPAIGQVLSLG
                     R"
BASE COUNT          530 a          730 c          790 g          499 t
ORIGIN      
        1 ccaggacctg ttttcgctca gccgaaatca ttacgacagg atcgggcact tcatgcaggg
       61 ctttgtcccg gcgctccttg cgcgcgagat actccttcgg ggcggctttg tgaccaaccg
      121 acgcatggcc gcctttcttg cggtatgcat tgccatggcg atcagcgctt ggtacgagct
      181 tatcgaatgg gctgcggccg taattctcgg gcagggcgcc gatgctttcc tgggcaccca
      241 gggagatccg tgggacacac aggaggacat gttcatgtgc ttcatcgggg cgatcgttgc
      301 cctgctgacc ctgtcgcggg agcaggacag gcagatggca aacttcacgc gatgaactct
      361 tcatacggaa gtattacctc ttttctttaa gcctctccgg ggtggtaaaa ttagagtatt
      421 agagagtgtt tggagaaaag aggtgaatgc ttatggtagt cgatctggaa aagaaggaag
      481 ttgatctgct cgtcaatgaa ctggagcacg ccaccattcc gaacctgagg gagtttatcg
      541 catcagggct gagggaggag cacagggaag agctgaaaca ggacgaggta gtactgaagc
      601 atctccttga aaagctcaaa atggccgcct gaccggaaca ggtccggcga aacgctgcac
      661 ggtgcaaagg gcagccctga gaaatcatga aaagggacgc agaagaggta tatgcgttcc
      721 tttttttatg aggtcctaat caatggtcga tatcaagctg gaggtcacga tctacgcggg
      781 ctaccgcggc tccgaacgtc ccaaatcttt cctgttcgaa ggaaacacgg tggaggtggc
      841 gggtatccgg caaatgtggg tcgaggaaga gcacaagacg aggatgcaga agcggttttt
      901 catgctcacg ggaaacgacg catacgaata cacgctctat cacgaactcg aatccgggga
      961 atggttcctg aggaagaggg aaaagaaata aggctgttgg gagtgcccct caaagatggt
     1021 catcgcccca gggaaagaac ctgcccgatc gccggaatga aatccttctt tcgcgacatg
     1081 acccgcggga ccgtgcaggt atcgttcacc accttcacgc caaacgcctt ctctacgatc
     1141 cacgcctcac cgcgaaggag aatccgctcc tgcaccttgc cgaggggatt taaaaccaga
     1201 agcacggtaa gatcgtagtt gttcgccgca cgcaggcgct ccagctccgc gaccagttcc
     1261 cgctcccggg cgtcgatctc cgcgcagtcg agcgacatca tctggctcac gccgagcctt
     1321 ttcccgccga tcaggaattc cttgaagtcc gccgcaatca gttccgccgc ggtcttgtcc
     1381 tcgagattga tgctcgcgtg caggagctcc cgggcatagg actggatgtc gacgcctgcg
     1441 atttcagcaa ggcggcgggc ctcctggcgg tcccgctccg tggtggtcga gagcgtcaga
     1501 agaagggtat ccgataaaac cccggaaagc aggagcccgg cgatctcctt cgggatgtga
     1561 gtctggtaga ggaacatgat ccccgcgacg accgtgcagg tgcttccgac ggggtcgttg
     1621 tacacgtaga tcggcgccgt ggtcgagatg tctcccacgc ggtgatggtc gatgatctcg
     1681 aggatctcgg cctcctcgat gccgtccacg gcctgcgaaa gctcgttgtg gtcgacaagg
     1741 accgctatct tgcgcaccgg cttcaggagg tccgtccggg ttatgaagcc gatcaggcgg
     1801 ttgtcgctgt ccaccacgac cacggaccgg tagtccgact ccacgatctt cgaccggacc
     1861 tccgccatgg gggtgtacag ccccacggtc gggcacgcgg tcgtcatgat cgatgacaca
     1921 ggctcggaca tggtcatgag ctgtaccgtg gcgaaggccg gatggcgcga ggaaatgagg
     1981 agcacatctt tctgccgtgc ggccgcgacg acttcgttgg caatcggcgc gccgtcggtc
     2041 acgatcaagg cggaacatcc cacccggatg agctcgagat gcacgtcgtt ctggtcgccc
     2101 gccaccacga catcgccggg gctcacccgg ttcagcatgg tcccgcgctg catggcagcg
     2161 atcaggaccc tgccggtcag gaccgatatc tgcttcgtgt tgctgatgac gcggccgtcg
     2221 agggtcgtga ggaggatgtc gagctcaatc ggcgccttcg aaaggtccgc gaagcccacg
     2281 ctgtcgatgt aatggcgggc gatgtccttc aggcccacca cgcctgcaag cctcccggat
     2341 tcgtccacga ccgggacgga ccggacgccc tgttcgcgca tcataagcgc cagtgtctgg
     2401 atggagtccc ggacgcctac ggcgatgggc cgcttgaggt cgagatcgct caccgttgcc
     2461 acgaggctgt cgatctccgc cgagggggga acgccgaact tctcaagcac gaaggcggtc
     2521 tcgtggttca gcttcccggc gcgcacggg
//
LOCUS       DASSLD010000233         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_242542,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000233 DASSLD010000000
VERSION     DASSLD010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242542"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /gene="lepA"
                     /locus_tag="VFK23_04265"
     CDS             complement(<1..1395)
                     /gene="lepA"
                     /locus_tag="VFK23_04265"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET7318334.1"
                     /translation="MASTNIRNFCIIAHIDHGKSTLADRILEYTGALSDREMAAAGTD
                     QVLDDMDLERERGITIKAHAVRLRYTASDGREYILNLIDTPGHVDFTYEVSRSMAACE
                     GALLVVDASQGVEAQTLANAYLAIDHNLEMIPVINKIDLPSANIEEVKRQIEDVVGLD
                     ASGAIPASAKEGIGTKEILEAVVKHVPSPAGNADAPLRALLFDSWYDTYQGVVILVRV
                     VDGSVRPKQKIQLYSNAAVYEVQSVGVFTPKMQQKSELSQGEVGCIIAGIKRVSDAKI
                     GDTIMDADRPADAPLPGYKDVKPMVFAGIYPVVSEAYADLRDALEKLRLNDSSFSYEP
                     ETSLALGFGFRCGFLGLLHMDIVKERLEREFKLDLIPTAPTVVYRVTTNKGEIVMVDN
                     PAKLPNAQDIQRIEEPIIMASLITPEQYVGSLIALCQERRGVQKEITYITKDRVLLTY
                     ELPLNEIVMDFYDKL"
     gene            complement(1537..2490)
                     /locus_tag="VFK23_04270"
     CDS             complement(1537..2490)
                     /locus_tag="VFK23_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7318335.1"
                     /translation="MGRVVIGVDLGGTNLRTALLSPDGDILAKHKEPTYASEGWKNVV
                     TRLIDSIKGQQESAGKRGLSVTAVGVGVPGVIQSDRGIVVKSPNFPDWNNLPIKDSLE
                     KALNLPVTVENDANAAALGEQWRGAGCGIKSMILLTLGTGVGGGIILNNQIWQGADGM
                     AGELGHMTIIPDGRRCGCGNTGCLEMYASARGIVQSYRERRVQALPSSGEITSEQVYE
                     AARTGEADALEVMQGMGRLLGIGIANLINIFNPEMIVIGGGVKDAWPLFIGTARDEIR
                     NRAFEVPAARTEIVPSRLGDDAGMVGAAAAALQNMEKKEKK"
     gene            2706..>3308
                     /locus_tag="VFK23_04275"
     CDS             2706..>3308
                     /locus_tag="VFK23_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET7318336.1"
                     /translation="MTAMQNRDFSVTLWLFLAATAVLVGGAAGYTVFSVADLPQVRNL
                     EEYQPSIISRVYADNHKLLAEFYLENRTPVALEDVPENLIKALIATEDVRFYSHWGID
                     YRGIARALYRNLRARRVIEGGSTLTQQLAKVLFLTPERSYARKLKELAVALRIEQCYT
                     KREILTFYLNQVYFGNGAYGVEAAARIYYGKSARDLNIAEC"
BASE COUNT          644 a          993 c          902 g          769 t
ORIGIN      
        1 cagtttgtcg tagaagtcca tcacgatctc gttcagcgga agctcgtagg tcaggagcac
       61 cctgtccttg gtaatgtaag tgatttcctt ctggacgccg cgccgttcct ggcaaagggc
      121 gatgagcgac ccgacgtact gctccggggt gatgagcgag gccatgatta tgggctcctc
      181 gatgcgctgg atgtcctggg cgttcggcag ttttgccgga ttgtccacca tcacgatctc
      241 gcccttgttc gtggtcaccc ggtagaccac ggtcggggcc gtcgggatga ggtcgagttt
      301 gaattcccgt tcgagccgct ccttcacgat gtccatgtgc agcagcccca gaaaaccgca
      361 gcggaaacca aaaccgaggg cgagcgacgt ctcgggttca tagctgaacg atgaatcgtt
      421 caaccgcaat ttttcgagtg cgtccctgag gtccgcgtag gcctcgctca cgaccgggta
      481 aatgccggcg aagaccatcg gcttcacatc cttgtacccg gggagcgggg catcggccgg
      541 gcggtcggcg tccatgatcg tgtcgccgat cttggcgtcc gaaacgcgtt tgatgccggc
      601 aatgatgcac cccacctcgc cctgcgatag ttccgacttc tgctgcatct tgggagtaaa
      661 cactcccacg gactggactt cgtaaacggc ggcattcgag tagagctgga tcttctgctt
      721 cgggcgcacg ctgccgtcca ccacccgcac gagaatgacc acgccctgat aggtgtcgta
      781 ccatgaatcg aacaggagcg cgcgaagcgg tgcgtcggcg ttccccgcgg gcgacggaac
      841 gtgttttacc accgcttcga ggatctcttt ggtgccgatg ccttctttgg ccgaggccgg
      901 gatggcccct gaggcgtcca gtcccaccac gtcctcgatc tgcctcttta cctcttcgat
      961 gttcgcgctc ggcaggtcga tcttgttgat taccgggatc atctcgagat tatggtcgat
     1021 cgccaaatag gcgttggcca gcgtctgggc ttccacgccc tgcgatgcgt ccaccacaag
     1081 gagcgcgcct tcgcatgccg ccatgctgcg cgagacttca taggtaaagt cgacgtgtcc
     1141 cggcgtatcg atcagattca ggatatactc ccggccgtcc gatgccgtgt atcgaagtcg
     1201 aacggcgtgg gccttgatgg taatgccccg ctcccgctcg aggtccatgt catccagcac
     1261 ctggtcagtg cctgcggcgg ccatttcccg gtcggaaagc gctcccgtgt actcaagaat
     1321 cctatcggcg agtgttgatt tgccgtgatc gatgtgtgcg ataatgcaaa agttcctgat
     1381 attcgtgctt gccatacttt ctgagaacca aattacaaat cccaaacccc aaatcacaaa
     1441 tacattccaa attcaaaaag tcaatatccg aaacgtttgg aattttgaat ttgatgtttg
     1501 gagcttattt gttatttgtt gattgatatt tggtgcttac tttttctcct tcttttccat
     1561 attctgcagc gctgctgccg cagcgccgac catcccggcg tcgtcgccga gacgcgacgg
     1621 gacgatctcc gtccgcgcgg cggggacttc aaaggcccga ttcctgattt cgtcgcgtgc
     1681 cgtgccgata aacagcggcc aggcgtcctt gaccccgccg ccgatcacga tcatttcggg
     1741 gttgaagatg ttgatgaggt tcgcgatgcc gatcccgagc aggcgcccca tgccttgcat
     1801 gacctccagc gcgtcggcct ctcccgttcg cgcggcttca tagacctgct cggacgtgat
     1861 ctctccggat gacggcaagg cctgtacccg ccgctcccgg tagctttgca cgataccgcg
     1921 tgccgacgca tacatttcaa ggcatccggt attgccgcac ccgcagcgtc tgccgtcggg
     1981 gatgatcgtc atgtggccga gttctcccgc catcccgtcg gcaccctgcc agatctgatt
     2041 gttcagtatg atccctccgc cgacgccggt accgagcgtc aacaggatca tgcttttgat
     2101 tccgcaaccc gcgccccgcc actgctcacc cagcgccgca gcattagcgt cgttttcgac
     2161 ggtgaccggc aggttgagag ccttctccag cgagtctttg atcggcaagt tgttccagtc
     2221 ggggaaattg ggcgacttca ccacaatccc gcggtcgctc tggataacgc cggggacacc
     2281 gaccccgacg gcggtcacgg agaggcctct cttccccgcg gattcctgct gccctttgat
     2341 actgtcgatc agccgtgtga ccacattttt ccagccttcg gatgcgtacg taggttcctt
     2401 gtgcttcgcc aggatgtcgc cgtcgggact gagaagcgcc gtcctcaggt ttgttccacc
     2461 gagatctaca ccgataacga cacggcccac acctgccctc ctaactactg aaaaaatatg
     2521 ggttatacga aagatgttta tactatcata ctgccgcaga aatgtaaata tttttattag
     2581 aattctctgt ccatcctatt ccctctcatc gaacatgccg tccatgcctt acaggctttt
     2641 catcgctgca atcgggcagt ggtctcattc ttcacacttt gcttttcggc agacagggat
     2701 gtataatgac cgccatgcaa aacagggatt tctctgtcac gctctggcta ttcctggcag
     2761 cgaccgccgt tctggtcggc ggggcggcag ggtatactgt attttccgtc gcagacctgc
     2821 cgcaggtacg caacctagaa gaatatcagc caagcatcat ctcgcgcgtc tatgccgaca
     2881 accacaagct tctcgccgag ttttatctgg agaacaggac gccggtcgcc ctcgaggatg
     2941 tccctgaaaa tctgatcaaa gcgctcatcg cgacggaaga cgtgagattt tacagccatt
     3001 ggggcatcga ttaccgcggg atcgctcggg cgctgtaccg gaacctccgt gcgcggaggg
     3061 tgattgaagg cggcagcacg ctgacgcagc aactcgccaa ggtgcttttc ctcactccgg
     3121 agcggagtta tgcgcgaaag ctcaaggaac ttgcggtcgc gctcaggatc gaacagtgct
     3181 atacaaaacg cgaaatcctt actttctatc tcaaccaggt ttatttcggc aacggcgcct
     3241 acggtgtgga agcggcggcc cgtatctatt acgggaagtc ggcaagggac ctgaatatcg
     3301 ccgaatgc
//
LOCUS       DASSLD010000234         2433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_551933,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000234 DASSLD010000000
VERSION     DASSLD010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551933"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFK23_04280"
     CDS             <1..198
                     /locus_tag="VFK23_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007362692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HET7318337.1"
                     /translation="SHWGMPVSTTHVVSSAILGVGATKRLSAVRWGIAGKIVMAWVLT
                     LPVCIGMAWGIQKLLIASHLG"
     gene            complement(195..827)
                     /locus_tag="VFK23_04285"
     CDS             complement(195..827)
                     /locus_tag="VFK23_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET7318338.1"
                     /translation="MKCKIDLHVHSKYSGDNDADPEEAVIRAIESGLQGIAFTEHYYY
                     EASEQAETLKEKYGGRILILRGVEFSADEGHCLVFGADTDKLGIKYAPVEDVIRFVSA
                     AGGVVIPSHPYRPGTSLGDLVRNMNGITGLEGYNGANMHSFNARAIKAAKAMHIPYTG
                     GSDAHEPREVGSCYTEFRHAVSYDNFIDSLKAGDYLGVDTRKISRLAGLF"
     gene            complement(1128..2330)
                     /locus_tag="VFK23_04290"
     CDS             complement(1128..2330)
                     /locus_tag="VFK23_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HET7318339.1"
                     /translation="MRTASTEFFSKLRAALSETFSAFQRNNDLTAASSLAFSATLALI
                     PSLFLLTFVLGAVIGSSADALARTQELLRQLIPAYSQDIVREVQSISSHLGAIGLVNS
                     LVLLWSITPLVADLRISLGTVFRKKPTRPFLLEKLFDVALGIVFLMGVSAIAVAGIVV
                     TLLETRTNLHIPLGWFERVVPFLFMVAVVFALYALFSKGVNKLNLLIGAVATSLLWFA
                     MRPLFHLFLLYNPGYGFAFGSFKSLFVVIIWIYFSLVVFLLGAEFAASLGRDETVFIK
                     KLMEGKKGVPARIISKYVLSREEGSVIFSEGETASEMYSVLKGRVSLRRGGMEIAVIT
                     EGKCFGGMSFLLSSPRMVEAVALEDVDLVSIDNENINKLMNEYPAYIIEILREMAERM
                     RDSNKVID"
BASE COUNT          554 a          681 c          691 g          507 t
ORIGIN      
        1 agccattggg gcatgccggt cagcacgacc catgtcgtct catccgcgat cctcggcgtc
       61 ggcgcgacga aaagactatc ggccgtccgc tggggcatcg cgggcaagat cgtcatggcc
      121 tgggtgctga cgctgccggt ctgcatcggc atggcctggg ggattcagaa actgttgatc
      181 gcctctcacc tcggctagaa gaggcctgcc agcctcgata tcttcctcgt atccacacca
      241 aggtaatctc ccgccttcag cgagtcgata aaattatcat acgacacggc gtggcggaac
      301 tcggtatagc aggagcccac ctcgcggggt tcatgcgcgt ccgatccgcc ggtatagggg
      361 atatgcattg ccttcgccgc cttgatcgcc cttgcgttga aggagtgcat gtttgcgccg
      421 ttgtatcctt caaggccggt aatgccgttc atgttccgca cgaggtcgcc gaggctcgtg
      481 cccggtcggt agggatggga cgggatcacg acgcctccgg ccgcgctgac gaagcgtatc
      541 acgtcctcaa cgggagcata cttgatgccc agcttgtccg tgtcggctcc gaataccagg
      601 cagtggcctt cgtctgccga gaactcgacg ccccgaagaa tcaggatccg tccgccgtac
      661 ttctccttca gggtctcggc ctgctccgac gcttcataat aataatgctc ggtgaaagcg
      721 atcccctgaa gaccggactc gatggcccgg attacggctt cctcgggatc ggcgtcgttg
      781 tcgccgctgt acttcgaatg aacgtgaagg tctatcttgc atttcatggg tataaggtat
      841 accacgtcgg cggctgcaat ggcaacatca aaatgcgtga tactaacacg gataaaacgg
      901 aactataatc ttggtaccgc caaggcgcta aggtcgcgaa ggaaagctaa attctttcgg
      961 gtttaaaaat caagaaagac ttcctttgcg ttcttcgcac cttcgcggta aatttttttt
     1021 ctctgagaat attttgattc tttagttact gaaaggagca cattgggacg cagataagac
     1081 cggatatgga aatctgcgtt catccgcgtc cgactgccgt ataaaaatca atcaatcacc
     1141 ttgttcgagt cccgcatccg ttccgccatt tcgcggagaa tttcgataat gtaggcgggg
     1201 tactcgttca taagtttgtt gatattctcg ttatcgatag agaccaggtc cacgtcttcc
     1261 agcgccaccg cctcgaccat gcgcggagac gatagaagga acgacatgcc tccgaagcac
     1321 ttcccctctg tgatcaccgc gatctccatt ccgccccttc tgagcgatac ccttcccttg
     1381 aggaccgagt acatctccga tgctgtctcc ccctcgctga agatgacgct tccttcttca
     1441 cgggacagga cgtacttgct gatgatcctc gcgggcacgc ctttcttgcc ttccatgagt
     1501 ttcttgatga agaccgtctc atcccgcccg aggctcgcgg cgaattccgc cccgagcagg
     1561 aacacgacga gggaaaaata tatccagata atcacgacga agagcgactt aaaggacccg
     1621 aaagcgaaac cgtagcccgg attgtacaga aggaacaggt ggaacagagg acgcatggcg
     1681 aaccagagaa gcgacgtggc cacggcgccg atgaggagat tgagcttgtt gacgcccttc
     1741 gagaacaggg cgtagagcgc aaagacaacg gccaccatga aaaggaaagg aacgacgcgc
     1801 tcgaaccagc caaggggaat gtgcagattt gtccttgtct ccagaagggt aacgacaatg
     1861 ccggccacag cgattgccga cacccccatc aggaacacga tgccgagggc gacgtcgaac
     1921 agcttttcca gtaggaaggg gcgcgtcggt ttcttccgga aaaccgttcc gagcgatatc
     1981 cggaggtccg caacgagcgg cgtgatgctc cagagcaaaa cgagggaatt caccaggccg
     2041 atggcgccga gatgggacga tatggactgg acctcccgca cgatgtcctg gctgtaggcg
     2101 gggatcagct gacgcagaag ttcctgcgtc ctggccaggg catccgccga cgacccgatc
     2161 acggcgccga ggacgaaggt aagcagaaag agagatggaa tgagcgcaag ggtggcggaa
     2221 aacgcgaggg atgatgccgc ggtcaggtca ttgttcctct gaaaggcaga gaacgtttcg
     2281 gacagggctg cccggagttt ggagaaaaat tcagtggacg ccgtgcgcat ggggcgtaaa
     2341 tgtctttcgt aatctgatcc ggctcttgtt taagctcccg atccgctgcc ggtcctcatc
     2401 cgtgatggcg acaaacttca gccccatacc ggg
//
LOCUS       DASSLD010000235         4770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_855129,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000235 DASSLD010000000
VERSION     DASSLD010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855129"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFK23_04295"
     CDS             complement(<1..350)
                     /locus_tag="VFK23_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7318340.1"
                     /translation="MIQVRPVYVICMREFIKFFREKSRLLGTLARPVLWLFVVGNGMS
                     SLIRPQTGFSYLQFIFPGMIGMTILFSSIFSSISIVWDREFGFMKEMLVAPISRLSIV
                     VGKALSGTLISVAQA"
     gene            complement(347..1330)
                     /locus_tag="VFK23_04300"
     CDS             complement(347..1330)
                     /locus_tag="VFK23_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7318341.1"
                     /translation="MSIITVQNLSKQFKTLKAVDGISFDVQEGEVFGFLGPNGAGKTT
                     TINILCTLLSPSSGSATIAGFDCTKDPDRVRTAIGIIFQDTTLDVGLTAYENLKFHAY
                     LYNLDRRLAEKRIDEMLSVVELQGRKHDLIKNFSGGMKRRLEIARGLLHYPRVLFLDE
                     PTLGLDPQTRNTIWDFIDALRKKEKITVFMTTHYMEEAENCGRIAIIDHGRIIALDTP
                     EKLKQMVHGDVIHVVTTDNMRAMEELQRNFHIAAREENGGLVLETEKGDEFIPRMIHA
                     LPVRTVSVSLKKPTLNDVFLKLTGRTIRDDVLQDSRETTREFVRSHRRAQR"
     gene            complement(1522..3057)
                     /locus_tag="VFK23_04305"
     CDS             complement(1522..3057)
                     /locus_tag="VFK23_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HET7318342.1"
                     /translation="MLPACGQKVERNPNTFVESSIGDARRLNPVIANDASSGEINDQI
                     FNGLVKYDKNIKLIGDLAERWTIENGGKTITFYLRKGVKWHDGVEFTAADCLFTYQKL
                     IDPNVATPYSSSYMDIKKAEVVGKYVFRVTYNEPFSPALESWAMGIIPKHLLEGRDIN
                     TADFNRHPVGTGPYRFREWLSAQKIVLDANDEYFEGRPNIDQFIYRIIPDSSTMFQEL
                     LFGGVDMMGLNPLQYLRKSESPRIRENYRKFRYPANAFTYMGYNLRSPLFSDMKVRQA
                     LSYAIDRQGIIEGILLGIGRPCTGPFSTVSWAYNPNAKSYEYDPERARRMLEEAGWKE
                     KSGGIREKNGRPFRFTIMTNQGNNERIRTAQIIQQNLKAVGIKVDIRVMEWQAFLEQI
                     DKRSFDVIILGWSMSRDPDLYDIWHSSKTRKGEYNFIGYSNPTVDRLLVAGRRTFDIE
                     KRKKIYYRIHAILAEEQPYAFLYVPDATPIVHKRFKGIEVAPLGIFYNFREWYVPKDR
                     MQW"
     gene            complement(3268..3609)
                     /gene="secG"
                     /locus_tag="VFK23_04310"
     CDS             complement(3268..3609)
                     /gene="secG"
                     /locus_tag="VFK23_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET7318343.1"
                     /translation="MHIFLVIVHVIVSAILIGMVLLQKGKGADIGAAFGGASQTVFGP
                     RGAQSFLAKLTTGAAIIFMFTSLGLALTTTKKSSIMPDVQPPAQTAPAVPLAPPMQSA
                     PAPAPAPIPAK"
     gene            complement(3721..4482)
                     /gene="tpiA"
                     /locus_tag="VFK23_04315"
     CDS             complement(3721..4482)
                     /gene="tpiA"
                     /locus_tag="VFK23_04315"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018999481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET7318344.1"
                     /translation="MNKTRMPIIAGNWKMNKTTAEARDLAAKLAPLVSGVKDREIVLA
                     PTFTALQTVAEAIRGTNMSLAAQNLHWEDKGAFTGEISAEMLLDLGCKYVIIGHSERR
                     QYFGETDETVNKKMKQALKKGLLPIMCIGETLTEREAGKLNDIITRQVTGGLKDISTA
                     DMKKVVIAYEPVWAIGTGKTATPEQAEEVHALIRQKVKALYDEGTAEGLRIQYGGSVT
                     PDNVSSLMAKPDIDGALVGGASLKPESFAALVNFK"
     gene            complement(4621..>4770)
                     /gene="pgk"
                     /locus_tag="VFK23_04320"
     CDS             complement(4621..>4770)
                     /gene="pgk"
                     /locus_tag="VFK23_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET7318345.1"
                     /translation="VGGGDTDVAVHRAGVSDSITFISTGGGASLELLEGKELPGLAAL
                     TDDRE"
BASE COUNT          879 a         1355 c         1420 g         1116 t
ORIGIN      
        1 gcctgggcga ccgagatcag cgtgcccgag agcgccttgc ccacgacgat ggagagacgg
       61 gagatgggcg ccacgagcat ctccttcatg aacccgaatt cccggtccca gacgatagag
      121 atggaggaaa agatcgacga gaacaggatg gtcatgccga tcatgccggg aaagatgaat
      181 tgcaggtacg aaaagcccgt ctgcggcctg atgagcgagc tcatgccgtt tccgaccacg
      241 aagagccaga gcacgggccg ggcgagcgtt cccaggagcc ggctcttttc gcgaaagaac
      301 ttgatgaact cgcgcatgca gatgacgtag acgggccgga cctggatcat cgttgcgccc
      361 tccggtggct ccgcacgaac tcgcgggtcg tttccctgct gtcctgcagt acgtcgtccc
      421 tgatcgtcct ccccgtgagt ttgaggaaca cgtcgttcag ggtgggcttc ttgaggctca
      481 ccgacacggt ccgcacggga agggcgtgaa tcatccgggg gatgaattcg tcaccttttt
      541 cggtttcgag gaccagcccg ccgttctcct cccgcgcggc gatatggaag tttctttgaa
      601 gctcctccat ggcacgcatg ttgtccgtgg tcaccacatg gatgacgtct ccgtgcacca
      661 tctgcttcag cttctccgga gtgtcgagag cgatgatcct gccgtggtcg atgatcgcga
      721 tgcggccgca gttttccgcc tcttccatgt aatgggtcgt catgaaaacg gtgatcttct
      781 ctttcttcct gagcgcgtcg atgaagtccc agatcgtgtt ccgcgtctgc gggtcgaggc
      841 ccagggtcgg ctcgtccaga aacagcacgc gcgggtagtg aaggagcccc cgggcgatct
      901 cgagacgcct cttcataccg ccggagaagt tcttgatgag gtcgtgcttg cggccctgga
      961 gttcgaccac ggaaagcatc tcgtctatcc gtttctcggc cagcctcctg tcgaggttgt
     1021 agaggtaggc gtgaaacttg aggttttcgt acgcggtcag gcccacgtcg agggtcgtgt
     1081 cctggaaaat gatgccgatg gccgtgcgca cccggtcagg gtccttcgtg cagtcgaatc
     1141 cggcgatcgt cgccgatccc gatgagggtg aaagcagcgt gcagaggatg ttgatggttg
     1201 tcgttttgcc cgcgccgttc ggcccgagga aaccgaagac ctcgccttcc tgcacatcga
     1261 acgagatgcc gtcgacggcc ttgagggttt tgaactgctt cgaaagattt tgtacggtga
     1321 tgatagacat gattttcaat aaagttcgta gtttgcagtt agtagtgagg tgaactactc
     1381 agtttgtcat tcccgcgcaa gcgggaatcc attggccttt tttcaaggtc gaattcaaaa
     1441 tggattcccg ataaaaagca ttcgggaatg acaggagggt tcgcagtcac tacgaactcc
     1501 caactcccaa ctccgaacca gctaccattg cattctgtcc ttcggcacgt accattcccg
     1561 gaagttgtag aagatgccaa gcggcgcgac ttcgattccc ttgaaccgtt tgtgcacgat
     1621 gggcgtggcg tcgggcacat agaggaaggc gtagggctgc tcctcggcca ggatcgcgtg
     1681 gatgcggtag tatatcttct tcctcttctc gatgtcgaag gtgcgccggc ccgcgaccag
     1741 caggcggtct accgtcgggt tgctgtatcc gatgaaatta tattcgccct tcctggtctt
     1801 gctcgagtgc cagatgtcat agaggtccgg gtcgcggctc atgctccagc ccaggatgat
     1861 gacgtcgaag gaccgcttgt cgatctgttc gaggaaggcc tgccattcca tgaccctgat
     1921 gtccaccttg atgcccacgg ccttcaggtt ctgctggatg atctgcgcgg tgcggatgcg
     1981 ctcgttgttt ccctgattcg tcatgatcgt gaaccggaag ggcctgccgt ttttttcccg
     2041 gatgccgccg gatttttctt tccagccggc ttcctcgagc atgcggcgtg cacgctccgg
     2101 gtcgtactcg tagctcttcg cgttcgggtt gtaagcccag gagacggtgg agaaggggcc
     2161 ggtgcaaggg cgtccgatgc cgaggaggat gccctcgatg atgccctggc gatcgatggc
     2221 gtacgagagc gcctggcgga ccttcatgtc cgagaacagg gggctccgca gattataccc
     2281 catatacgtg aacgcgttcg cgggataccg gaacttgcgg taattctccc tgatgcgggg
     2341 cgattcgctc ttcctcaggt actgcagggg gttcagaccc atcatgtcca cgccgccgaa
     2401 gagcagttcc tggaacatgg tggagctgtc ggggatgatg cggtagatga actggtctat
     2461 gttcggcctg ccttcgaagt attcatcgtt cgcgtcgagc acgattttct gggccgagag
     2521 ccattccctg aaccggtaag gccccgtgcc aacgggatgg cggttgaagt cggcggtgtt
     2581 gatgtccctg ccttcgagca ggtgcttggg gatgatcccc atggcccagc tttcgagggc
     2641 cggggaaaag ggctcgttgt aggtcacacg gaagacgtat ttgcccacca cctccgcctt
     2701 cttgatgtcc atgtaggagc tgctgtaggg cgtggccacg ttggggtcga tgagcttctg
     2761 gtaggtaaag agacagtccg ccgcggtgaa ttcaacgccg tcgtgccact tgacgccttt
     2821 gcggagatag aaggtgatcg ttttgccgcc gttttcgatg gtccaccgct cggccagatc
     2881 gccgatgagc ttgatgttct tgtcgtactt taccagtccg ttgaagatct ggtcgttgat
     2941 ctcgcccgat gaggcgtcgt ttgcgatgac cgggttcaac cgcctggcgt ccccgatgga
     3001 actctcgacg aaggtgttcg ggttccgctc gaccttctgg ccgcaggcgg ggagcataca
     3061 ggatgcgagt gcgagagcca gcggcaacca tcgacgacgc agtgaaggac ggattccttt
     3121 tttcaggaaa gaaatgaatc tccctgtgtt cctctgcgac ctctgtggta gatgtctttt
     3181 ttccgggatg tcgtttttca taataaaaaa gaaacgggta ggcagggcct acccgttcac
     3241 gcgtcacatc aagcgttcat gccggaacta ttttgccggg atgggcgccg gagcgggcgc
     3301 gggcgccgac tgcatgggcg gggccagagg gaccgcgggg gccgtctggg ccggcggctg
     3361 aacatccggc atgatcgagc tctttttcgt tgtcgtcagg gcgagtccga gcgaggtgaa
     3421 catgaaaatg atcgctgcgc cggtggtcaa tttggcaaga aagctctgcg ctcccctcgg
     3481 gccgaaaacg gtctgtgacg ctccgccgaa ggccgcgccg atgtccgcgc ccttgccctt
     3541 ctgcagcagg accattccga tcaggatggc cgagacgatg acatgaacta ttaccaggaa
     3601 tatatgcatt gctttctcct tatgttctct ttatcgcgga gacgccgaga cacagagaaa
     3661 aaattataat cagattgaac tgttttctct gcatcctctg cggctctgcg gttaaaaacg
     3721 ttacttgaag ttcaccagcg ccgcgaacga ttccggcttc agactcgctc cgcccaccag
     3781 tgccccgtcg atgtcgggct ttgccatgag cgaagacacg ttgtccgggg tcacgctccc
     3841 gccgtactgg atgcgaaggc cttcggcggt tccctcgtca tacagggcct ttaccttctg
     3901 gcggataagg gcatggacct cttccgcctg ctcgggagtc gccgttttcc ccgtgccgat
     3961 ggcccagaca ggttcgtagg cgatgacgac ctttttcatg tctgcagtgc ttatgtcctt
     4021 caacccgccg gttacctgtc tcgtgatgat gtcgttcagc ttgcccgctt cgcgttcggt
     4081 cagggtctcg ccgatgcaca tgatcgggag cagtcccttc ttgagcgcct gcttcatctt
     4141 cttgttcact gtttcgtcgg tctcgccgaa gtactgcctg cgctcactgt ggcctataat
     4201 aacatacttg catcccaaat ccaacagcat ttcagcggaa atctcgccgg tgaaggcccc
     4261 tttgtcctcc cagtggaggt tctgggccgc aaggctcata ttcgtgcccc tgatggcctc
     4321 ggccacggtc tgaagggccg tgaatgtcgg ggcaagcacg atctcccggt ccttgacgcc
     4381 cgacacgagc ggcgcaagct ttgcggccag gtcccgcgcc tcggccgtcg ttttgttcat
     4441 cttccaattg ccggcgatga ttggcattcg tgtcttgttc atatgacatc cttctttccg
     4501 ctgatcgcgc aatgattgcg attcgagcca cgaaggtgag aaggtaggaa ggtgagaagg
     4561 taagagcaat cacactgctt tcacaccttc tcaccttcct accctcctac cttcaggtta
     4621 ttattcccta tcgtccgtca gggccgcaag gcccggcagt tccttgcctt ccaggagttc
     4681 gagcgatgct cctccgccag tggaaatgaa ggtgatactg tcggacacgc ccgcccggtg
     4741 cacggccacg tccgtatcgc cgccgcccac
//
LOCUS       DASSLD010000236         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_890391,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000236 DASSLD010000000
VERSION     DASSLD010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890391"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VFK23_04325"
     CDS             complement(<1..453)
                     /locus_tag="VFK23_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET7318346.1"
                     /translation="MKCTGRISFIGIALIVVLPFIAGPVAAAETKPNILVLYGDDIGQ
                     ANLSAYTMGLMGYRTPNIDRIAKEGMIFTDYYGEQSCTAGRSAFITGQSVFRTGLSKV
                     GVPAAPEGMNALDPTIAELLKPLGYATGQFGKNHLGDRNEHLPCVHGFD"
     gene            complement(804..>2145)
                     /locus_tag="VFK23_04330"
     CDS             complement(804..>2145)
                     /locus_tag="VFK23_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HET7318347.1"
                     /translation="KAYPVNYDLWKKTEEPTPAGKSKYKRGFNPDKITYDKLSEFPYM
                     ALLFNGWGFGVEYNEPRGHAYMMKDQINIDPSRVKAGGVCLTCKTPYAPKLEKEKGLE
                     YYSKPWSEVHAMIPAPHREIGVACIDCHDPKDMSLKISREFTLSKALESIKVEPGKLS
                     RQDMRSLVCAQCHVTYSIRKDENMKSVGMYFPWQGSKWGNISVENVIRQIRNDPSVGE
                     WKQNVTGFKLAFIRHPEFELFSANSVHWKAGVSCADCHMPYTKVGAYKVSDHRVTSPL
                     KNDLKACMQCHTESADWLRDQVITIQDRTASLMLRSGYATATAAKLFEMANDAEAAGK
                     KIDKELYAKAKDYYMEAFYRTSYVGAENSVGFHNPTEATRILGDATAFAGKAEGLLRQ
                     ALAKAGINVPEVVNLELNKYLEGRGQKKLVFQKNVEFKDPFGVQEKLLTVQQTK"
BASE COUNT          375 a          626 c          621 g          523 t
ORIGIN      
        1 gtcgaaaccg tgcacgcagg gaaggtgctc gttcctgtca cccagatggt tctttccgaa
       61 ctggccggtg gcgtagccta aaggtttcaa cagttcggca atggtcgggt ccagggcgtt
      121 catcccttcc ggcgccgccg gcacaccaac cttgctcagg ccggtgcgga acacgctttg
      181 gccggtgatg aacgccgaac gtcccgcggt gcagctctgt tctccgtagt agtccgtgaa
      241 gatcatgccc tccttggcga tgcgatcgat attcggtgtg cggtacccca tcaggcccat
      301 cgtgtaagcg ctgagattgg cctgtccgat atcgtctcca tagagaacaa ggatgttggg
      361 tttcgtctcc gctgccgcta cgggcccagc gatgaaaggc aacacaacga tcagggcgat
      421 gccgataaac gatatcctgc cggtgcattt cattgcttca ccccccttgt tctccggtcc
      481 acttcaccag ttcgatacca cccggccttc cggggcttgc ggcccgaaat ggagatcgag
      541 ccggtcggat gttccctcca ttatataaat aaaagattct tatgtattta tataatgcat
      601 ccgggaagcc cgggtcaatt ctgatgacgt acctgccggt ggttcgtgtg gcgatggcat
      661 gtaaagcgta aatcttcctg acttctccac cggttatcga tgggaagccg ggcggtccgc
      721 acccccgtct ttcccgcggg gggcggtctc gagagtacgc aggctgggca ggatggccca
      781 gcctgcgtat ggcgctgagg gggctatttc gtttgctgaa ccgtcaggag cttttcctgg
      841 accccgaaag gatccttgaa ttccacgttt ttctggaaga cgagcttctt ctgccccctg
      901 ccttcgagat acttgttcag ttcgagattc accacctccg gcacgttgat gccggccttt
      961 gccagcgcct ggcggagaag gccctcggct tttccagcga aggccgtcgc gtctccgagg
     1021 atcctcgtgg cctccgtcgg gttgtggaag ccgacggagt tttccgctcc gacataggaa
     1081 gtccggtaga aggcctccat gtaatagtcc ttggccttcg cgtagagctc cttgtcgatc
     1141 ttcttgcccg ccgcttccgc atcgttcgcc atctcgaaca gcttggctgc cgtggcggtg
     1201 gcgtacccgg agcgcagcat gagggatgcg gtccggtcct ggatcgtgat cacctggtcc
     1261 cgtaaccagt cggcggattc ggtgtggcac tgcatgcagg ccttcaaatc gtttttcagg
     1321 ggactcgtca cgcgatggtc ggaaaccttg taggcgccga ccttggtgta cggcatgtgg
     1381 cagtccgcgc aggagacgcc ggccttccaa tgcacgctgt tggcggagaa aagctcgaat
     1441 tccggatggc gaatgaaggc cagcttgaag cccgtcacat tctgcttcca ttccccgacg
     1501 gacggatcgt tgcggatctg ccggatgacg ttctcgacgg aaatgtttcc ccatttgctc
     1561 ccctgccacg ggaagtacat gccgacggac ttcatgttct cgtctttccg gatgctgtag
     1621 gtgacgtggc actgggcgca gacgaggctg cgcatatcct ggcgggaaag cttccccggt
     1681 tcaaccttta tgctttccag cgctttcgag agggtgaact cccgggagat cttcagggac
     1741 atatccttcg ggtcatggca gtcgatgcag gcgaccccga tctcccggtg aggtgccggg
     1801 atcatcgcat ggacttcgct ccatggcttg ctgtagtact ccagtccctt ctccttctcc
     1861 agcttcgggg cataaggagt cttgcacgtg aggcagacgc cgccggcctt cactctcgag
     1921 ggatcgatat tgatctgatc cttcatcatg taggcgtgtc cccgtggttc gttgtattcg
     1981 accccgaacc cccaaccgtt gaacaggaga gccatgtaag gaaattctga cagcttgtcg
     2041 taggtgatct tgtccgggtt gaaccctctt ttgtatttgc ttttcccggc gggcgtcggt
     2101 tcctcggtct tcttccacaa gtcgtagttg acgggatacg ccttt
//
LOCUS       DASSLD010000237         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1408880, whole genome shotgun sequence.
ACCESSION   DASSLD010000237 DASSLD010000000
VERSION     DASSLD010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408880"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VFK23_04335"
     CDS             complement(<1..708)
                     /locus_tag="VFK23_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HET7318348.1"
                     /translation="MSPDRIKTVYVVGHTTPDTDSVCSSIAYAYFKNATDKRFLYAPA
                     RAGTLNHETVFVLEKFGVPPSAELDSLVATVSDLDLKRPIAVGVRDSIQALALMMREQ
                     GVRSVPVEDESGRLAGVVGLKDIARHYIDSVGFADLQKAPIELDILLKTLDGRVISNT
                     KQISVLTGRVLIAAMQRGTMLNRIRPGDVVVAGDQNDVQLELIRMGCSAVIVTDSAQI
                     SSEVAAAAREKEVLLISS"
     gene            973..1911
                     /locus_tag="VFK23_04340"
     CDS             973..1911
                     /locus_tag="VFK23_04340"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET7318349.1"
                     /translation="MLQATDKIWMNGSFVRWDDAKVHILTHSLHYGTGVFEGIRCYKA
                     DAGSAVFRLPEHVNRLFDSAHILQMEMPFTREVVSEAILETIRINKIDACYIRPLAYI
                     GYGAMGVFPKENPVQVSIAVWPWGSYLGDDGIQNGIRVKISSFSRPHVNATMVRAKTT
                     ANYANSLLAKREALKDGYDEAMLLDTDGYVAEGSGENVFMVRKGVIKTPPLTSILEGV
                     TRDTVMQLAAERGMRLVEERFTRDELYVADEAFFSGTAAEITPIREVDNRAIGAGKPG
                     PVTKELQAVFFDIVHGRDSKHADWLTPVSKQAAVKR"
     gene            2081..2165
                     /locus_tag="VFK23_04345"
     tRNA            2081..2165
                     /locus_tag="VFK23_04345"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Leu,seq:gag)
BASE COUNT          489 a          699 c          694 g          488 t
ORIGIN      
        1 agacgagatg agaagcacct ctttttcccg ggccgccgca gcgacctcgc tggaaatctg
       61 ggcgctgtcg gttacgatga cggccgaaca acccatcctg atgagctcga gctgcacgtc
      121 gttctggtcg cccgcgacca cgacgtcccc gggacgtatc cggttcagca tggtcccgcg
      181 ctgcatggcg gcaatcagga ccctcccggt caaaaccgag atctgcttcg tgttgctgat
      241 gacgcggccg tcgagcgtct ttaggaggat atcgagctcg atcggcgcct tctggaggtc
      301 agcgaagccg acgctgtcga tgtaatggcg ggcaatgtct ttgaggccca cgacgccggc
      361 cagcctgccg gactcgtcct cgaccggtac cgagcggaca ccctgctcgc gcatcatgag
      421 cgccagggcc tggatggagt cgcggacgcc cacggcaatg ggccgcttga ggtcgaggtc
      481 gctcaccgtg gccacgaggc tgtcgagctc ggcggagggg gggacgccga acttttcgag
      541 cacgaagacg gtctcgtgat tcagcgtccc ggcgcgcgcg ggggcgtaga ggaaccgctt
      601 gtccgtcgcg ttcttgaagt aggcgtaggc gatggaagag cacaccgaat ccgtgtccgg
      661 cgtcgtatgt ccaaccacat agactgtttt gattcgatca gggctcatac agtttccttt
      721 cggcaaggct gcccaatccg ccgggtgagt catctcttcc cccttcacgg tcctttgccc
      781 gactcaatcc gcgtttatcc ggtttaatcc gcgtccaaat gcctcttcga gaatccatat
      841 accagtatta ccgtaccgcg gcgagggtgt caagcggtaa tcggtccgcg tccggggaat
      901 gtacgaaaaa cagccgataa tccgcaaaaa aacgattgac aattccatgc cgggctgtta
      961 taaatgtcgt ctatgcttca ggcaacggac aagatatgga tgaacggttc cttcgtcagg
     1021 tgggacgatg ccaaggtcca tatcctgacc cattcgctcc actacgggac cggggtattc
     1081 gaaggcatcc gctgctacaa ggccgatgcc ggttccgcgg tattccggct gccggagcat
     1141 gtgaaccggc tgtttgactc agcgcacatc ctgcagatgg agatgccctt cacccgggag
     1201 gtcgtgagcg aggccatact cgaaacgatc cgcatcaaca agatcgacgc gtgctatatc
     1261 aggccgcttg cgtatatcgg ctacggcgcc atgggggtct ttcccaaaga gaaccccgtg
     1321 caggtgtcga tcgcggtctg gccctggggc agctatctcg gagacgatgg catccagaac
     1381 ggcatccgcg tcaagatatc gtcgttttcc cggccccatg tgaacgcgac catggtccgc
     1441 gccaagacaa cggcgaatta cgcgaactca ctcctggcca agcgcgaggc gctcaaggac
     1501 ggctacgacg aggcgatgct gctcgacacc gacggttacg ttgccgaggg cagcggcgag
     1561 aacgtgttca tggtccgcaa gggagtgatc aagaccccgc cgctcacctc gatcctcgaa
     1621 ggcgttaccc gggacacggt catgcagctc gcggcggagc gggggatgag gctggtggaa
     1681 gaacggttta cgcgtgacga actgtatgtc gccgacgagg ccttcttttc cggtaccgcg
     1741 gccgagatca cgccgatccg cgaggtggac aaccgcgcca taggcgcggg caagccgggg
     1801 ccggtgacca aggagctgca ggcggtcttc ttcgacatcg tccatggccg agatagcaag
     1861 cacgcggatt ggcttacccc ggtctccaaa caggccgcgg tgaagaggta aattttttta
     1921 ccacggtgtt cacaaaccga ggaaatacta aatacgaagc tcgaaattct aaataatatc
     1981 gaatgccgaa aaaaaataat tagaacattt gaattttacg attgtttagg gtttagtgct
     2041 tcgtgcttca aatttttccc tgtaattagt ttcagcaagt gccgaagtgg tggaactggt
     2101 agacacgcac gtttgagggg cgtgtggggc aacccatggg ggttcgagtc cccccttcgg
     2161 caccatcttt tcaccggaac acacgacgcg gcttttcgtc agtccactcc gttttcctga
     2221 ttcatcctga tgaaaaaact tctccccata ctaatcctga tctggtccgt cgccctcttt
     2281 tttccggcgg ccgggcttgc cctgcctccg caaaacagcg gcttcggaat cgtcaccgcc
     2341 cccctggcca atgttcacgc cgagcccctg
//
LOCUS       DASSLD010000238         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_373571,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000238 DASSLD010000000
VERSION     DASSLD010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373571"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /gene="coaBC"
                     /locus_tag="VFK23_04350"
     CDS             complement(<1..628)
                     /gene="coaBC"
                     /locus_tag="VFK23_04350"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HET7318350.1"
                     /translation="MERRDMLSGKRILLGVTGSIAAYKAADILRRLIERGAEVRVAMT
                     RHAAQFISPFTFETLSGMPVLCDEFAPSERTSIAHIDAPNGLDAALIAPATANIIGKI
                     ASGIADDALTSALMALGCPLLIAPAMNDRMYCNAVLQRNIAALRELGVRFIEPGTGSL
                     ACGVRGQGRLADTDRIIGELSAMFLSKDLSGKTVLVTAGPTRESIDAVR"
     gene            complement(634..1068)
                     /locus_tag="VFK23_04355"
     CDS             complement(634..1068)
                     /locus_tag="VFK23_04355"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET7318351.1"
                     /translation="MQKTAADLISLPIEMNDKIADTKFRLVHIASQRVRQLSAGAPPV
                     VISRSIKDATVALEEAVAGSFKILVGEEAKKAKEEFQKREEQARIEEQLSAKEEEIRK
                     ELSAYLSESQEEEGAEEALGEEPVEEEAEESTEEEAAEEAEE"
     gene            complement(1214..1822)
                     /gene="gmk"
                     /locus_tag="VFK23_04360"
     CDS             complement(1214..1822)
                     /gene="gmk"
                     /locus_tag="VFK23_04360"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET7318352.1"
                     /translation="MKRTRGLLFVVSAPSGAGKTSLCQSVTGSLENLTHSISYATRKP
                     RPGEIDGRDYHFVGTERFQEMVKAGDFAEWAEVHSNLYGTSRRVIDDMIAKGLDVILD
                     IDTQGAAQIKAKYDDAVFIFIMPPSLDILEERLRNRKSDHEDEIRKRMRRAREEIKDF
                     RMYDYVIVNRDFDRALSELRSIVIAERCRTRLADTEWIERMM"
     gene            complement(1819..2070)
                     /locus_tag="VFK23_04365"
     CDS             complement(1819..2070)
                     /locus_tag="VFK23_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HET7318353.1"
                     /translation="MINNVVNIGYGNMVMASKVVAVLSPDSAPMRRLKEEAKERKMLI
                     DATQGRKTRAIIITDSDHIILSAVQVETLMQRFLERGDQ"
     gene            complement(2179..>2376)
                     /locus_tag="VFK23_04370"
     CDS             complement(2179..>2376)
                     /locus_tag="VFK23_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HET7318354.1"
                     /translation="LSQFRAVLQESSPEGVGRRLDFLIQEMGRETNTIASKAMDAGIS
                     LDVVNIKAELEKVREQIQNIE"
BASE COUNT          410 a          747 c          644 g          575 t
ORIGIN      
        1 acctgacggc atcgatggat tcccgggtcg ggcctgctgt cacgagcacc gtcttgccgg
       61 aaaggtcttt cgacaggaac atcgcggaca gctctccgat gatccggtcg gtgtctgcga
      121 gtctgccctg ccccctcacg ccgcaggcaa ggctccccgt ccccggctcg atgaacctta
      181 cccccagttc cctcagggcc gcgatattcc tctgcagaac cgcgttacag tacatccggt
      241 cgttcatggc cggcgctatg aggagcgggc aaccgagcgc catgagggcc gaggtcaggg
      301 catcatcggc gatccctgag gcgatcttgc cgatgatgtt ggccgtcgcc ggagcgatga
      361 gggccgcatc aagaccgttc ggtgcgtcga tgtgcgcgat agaggtccgt tcggagggag
      421 cgaactcatc gcacagcacc ggcatgccgg acagcgtttc gaaggtgaag ggggatatga
      481 actgggccgc atgcctcgtc atggctacgc ggacctctgc gccccgctcg atgagcctgc
      541 gcaaaatgtc cgccgccttg tacgcggcga tgctgcccgt cacgccgagc agtatccgct
      601 tccccgatag catatcgcgc cgttccaccc gtgctattcc tcggcctctt ccgccgcttc
      661 ttcctcggtg ctctcctcgg cctcttcctc gaccggttct tcgccgaggg cctcttccgc
      721 gccctcttct tcctgagatt cggagagata agcgctcagt tccttgcgaa tttcctcttc
      781 cttggcggag agctgttcct cgattctggc ctgttcttcc cgtttttgga actcctcttt
      841 ggccttcttc gcctcctcgc cgacgagtat cttgaagctt cccgcaacgg cctcctcgag
      901 ggcaacggtc gcgtccttga tggaccgtga aatgaccacc ggcggggccc cggcggagag
      961 ctgtcttacc cgctgcgatg cgatatgcac caggcggaac ttggtatcgg cgatcttgtc
     1021 gttcatttcg atggggagtg aaattaaatc tgctgctgtc ttttgcatcg gacacctcct
     1081 tggattttcg attgatgatt gatgattgat gatcgatgat tgatgatcga tgattgatac
     1141 ggcattgctg attgcaggtc cctgagtgct tccgccctgc aatcgacaat catcgatcaa
     1201 caatgatcaa gccctacatc attcgctcga tccattccgt atcggccagc cgggttctgc
     1261 accgttcggc gatcacgatc gatctgagtt cggacagcgc ccggtcgaaa tcgcggttga
     1321 cgatgacata gtcgtacatc ctgaaatcct ttatctcctc ccgcgccctg cgcatccgtt
     1381 ttcttatctc atcttcatgg tcggacttgc ggttgcgaag cctctcctcc agtatgtcga
     1441 gcgaaggcgg catgatgaaa ataaacacgg catcgtcata tttcgccttg atctgcgcgg
     1501 cgccctgcgt gtcgatgtcg aggatgacgt cgagtccttt tgcgatcatg tcgtcgatca
     1561 cgcggcggga cgtcccgtac aggttggaat ggacctccgc ccattccgca aaatcgccgg
     1621 ccttcaccat ctcctggaac cgctccgtgc cgacgaagtg gtagtccctc ccgtcgatct
     1681 cgccgggccg gggcttccgc gtggcatagg atatggagtg cgtaagattt tccagtgatc
     1741 cggtgacgga ctggcacagg ctcgtcttgc cggctcccga cggcgccgat acgacgaaca
     1801 ggaggccccg cgttctcttc actgatcacc tctttccaga aaccgctgca taagggtctc
     1861 cacctgcacg gccgagagga tgatatggtc gctgtcggtg ataatgatcg cacgcgtctt
     1921 tctcccctgt gtcgcgtcga tcagcatctt ccgctccttt gcctcctcct tcagcctccg
     1981 catcggcgcc gagtcgggcg agaggaccgc cacaactttg gacgccatga ccatgtttcc
     2041 atatccgatg ttgacgacat tattgatcat agcttgcgtc tcatcgttcc tcgcctgaag
     2101 gtgaatcagt tcccggcccg cggttcacgg acgactgttc aatcgcgagc catcaattac
     2161 gggctgcgcg ctgattttct attcgatgtt ctgaatctgt tcccggacct tttccagttc
     2221 cgctttgata ttgacgacat ccagggaaat ccctgcatcc atcgccttgg aggcgatcgt
     2281 gttcgtctcc ctgcccattt cctggatcag gaaatcaagc ctcctcccga ccccttcggg
     2341 cgaagactcc tgcagtacgg cgcggaattg tgacag
//
LOCUS       DASSLD010000239         2737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1245494, whole genome shotgun sequence.
ACCESSION   DASSLD010000239 DASSLD010000000
VERSION     DASSLD010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245494"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="VFK23_04375"
     CDS             <1..1542
                     /locus_tag="VFK23_04375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7318355.1"
                     /translation="PSSTSPWSPDCAFLFFSARIALPANWHAPFLFTLSFVAVLPFWS
                     EPELIVDASRYFTQAKHLEVYGLGFFLREWGRTIQAWTDSPLVPLLYGLLFKAFGEHR
                     FVIQLFTTTLFSLTAVITYLIGKTLWNRETGFSAGLLLLGIPYLLTQVPLMLVDVPTM
                     FFLTLAVFMFLDAAGRGGRLRMTAAVAAIVLAALSKYSAWLMLSVLVVALAVFTVGGA
                     TLRDFRPARRGIVILLAATALACLILLFKSDIVIGQLALLRAYQKPGLDRWTESFRST
                     FLYQVHPFITLSALASVVVAARKRDIRFAVVCWLVLLVLALGIRRIRYILPLFPMLTL
                     MAGYGLQLIRREELKRFIVFGTAAASLSVGYFAYLPLAGSMSVTNLKHAGGFLNTLDV
                     PEVEVITLASRDPVANPAVAVPLLDLFTDKHIVYHYVPDSFPAREEVERSSLRFTWEY
                     RNPAYYSDERRALGAAPIVVISGASNGPLPITVSQRIEGRRLAEEFIADENIFQYTVG
                     VRIYH"
     gene            1955..>2737
                     /locus_tag="VFK23_04380"
     CDS             1955..>2737
                     /locus_tag="VFK23_04380"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HET7318356.1"
                     /translation="MIIISLLYYKANVYYMYLVAYIWFGIAYGMMLQYGRFCFASASR
                     DLFAAGVPRMAVGILIALIFFSVIQATLQATNMSTFHPAPFGIHTLLSGMIFGVGMVL
                     AGGCASGSLYKIGEGNGTSILSILGISFGQAIFVDVGGPFLKLVPDAWKVSSAAKNLP
                     PDKVRSWFDFYLSGYVWDKPSIQLSHTQTFSNALPGASKFFVADALISAIIPALILIV
                     VIYAFYIRKGFLKKRAKQTGQPTSFSDNFAGIWRMLTASKRTS"
BASE COUNT          553 a          794 c          682 g          708 t
ORIGIN      
        1 ccctcgtcta cctcgccctg gtcgccggac tgtgcatttc tttttttttc ggcacggatt
       61 gcgcttcccg ccaactggca cgcgcccttt ctgttcacgt tgtcctttgt cgccgtcctg
      121 ccgttctgga gtgagccgga actcattgta gacgcttctc gctatttcac ccaggcgaag
      181 catctcgagg tgtatggtct cgggttcttc ttgcgggaat ggggccggac tatccaggcc
      241 tggactgatt caccgctcgt gccgctcctc tatgggctgc tctttaaggc attcggagaa
      301 caccggttcg tcattcaact gtttaccacg acgcttttct ccctgacagc cgtcatcacc
      361 tacctgatcg ggaaaacact gtggaaccgg gaaacgggat tttccgccgg ccttctcctg
      421 cttggcatac cctacctctt aacgcaggtc ccgctcatgc tcgtcgatgt gccgaccatg
      481 tttttcctca cgctcgccgt cttcatgttc ctggacgcag cggggagagg cggccgcctc
      541 cggatgacag cagcggttgc ggctatcgtt ttggctgcac tgtccaagta ctccgcctgg
      601 ctcatgctct ccgtgctcgt cgtggcgctc gccgttttta cagtgggcgg cgcaaccctg
      661 cgggacttcc ggcccgcccg caggggtatt gtcatcctgc tcgccgctac ggcgcttgcc
      721 tgcttgatcc tgttgtttaa atccgatatc gttatcggcc aactcgccct cctgcgcgcg
      781 tatcaaaaac cgggcctcga ccggtggacg gagagcttcc gctcgacatt cctttaccaa
      841 gtccatccgt tcataaccct ctcggcgctg gcgtccgtcg tcgttgcagc acggaaaaga
      901 gacatacgct tcgccgtagt ctgctggctc gtcctgcttg tcttggcgct cggcatcaga
      961 aggatacggt atatcctccc cctgtttccc atgctgacgc tcatggccgg ctacggactg
     1021 cagttaatca gacgggagga gttgaaacgc tttatcgttt tcggtacggc agccgcatca
     1081 ctgagcgtgg gttatttcgc atacctgccc cttgccggaa gcatgagcgt cacgaacctg
     1141 aaacacgccg gcgggtttct gaatacgctc gacgtcccgg aagtcgaggt cattacgctt
     1201 gcgtctcggg atcctgtcgc caatcccgcc gttgcagttc cccttctcga cctgttcacg
     1261 gacaagcata ttgtatatca ctacgtgcct gacagcttcc ctgcccgcga ggaagtagaa
     1321 cggtcgtctc ttcgtttcac atgggagtac cggaacccag cgtattacag cgatgaacgt
     1381 cgcgcgctgg gagctgcccc catcgtcgtc atttccggtg cctcgaacgg ccccctgcca
     1441 ataaccgttt cgcaaagaat cgagggccgc cgattggccg aagagttcat tgccgatgaa
     1501 aatattttcc agtacacggt cggagtcaga atctatcatt agcacttcgc gaaattttgc
     1561 acccgaagcc gaaaaaaacc tggacacggg acattgcaaa attacgtttt ttattgcaat
     1621 atcaagcaac tatgcaacat atagggaaaa ataatgcttg ccatggatta ttgattgttc
     1681 tattatataa gcctacaggt aaattacttc tcagaatttt gttaaccgct tttttcagtt
     1741 caatttaatg ttaaccgtgt ttaaaacaaa atatgctctt tacagttagc gttaacgtta
     1801 acagcgaaag gggggaaggc actctcaatc tacaataaag aaggcacaac caagataggc
     1861 ttctgagctt tgtctttcaa cgctatttta agggggaaat agtattatgg aagagaaaag
     1921 aaatcagata ttggcctttg cggtgacagg tttgatgatt atcatcagcc tcctttatta
     1981 caaggcaaat gtatactaca tgtatctggt ggcgtacatt tggttcggaa tcgcatatgg
     2041 tatgatgctt cagtacggca ggttctgttt cgcctctgct tcacgggacc tgttcgccgc
     2101 cggcgttccg agaatggccg tcggcatcct catcgcgctc atctttttca gcgtcattca
     2161 ggcgacgctc caggcgacca acatgagcac gttccatccg gccccgttcg gcatccacac
     2221 cctgctgtcg ggcatgatct tcggcgtcgg gatggtcctt gccggggggt gcgcctcggg
     2281 gtccctgtac aaaatcgggg aaggtaacgg cacctcgatc ctctccatcc tcggcatatc
     2341 cttcggccag gccatcttcg ttgacgttgg cgggcctttt ctgaagctag tgcccgatgc
     2401 ctggaaggta tcatcggcag cgaaaaatct tcctcccgat aaagtcaggt cctggttcga
     2461 cttctacctc tccggctatg tctgggacaa accatccatc cagctttcgc acacgcaaac
     2521 cttctcaaat gccctcccgg gagcctcaaa gttcttcgtt gcggacgcgc tcatcagcgc
     2581 gatcattccg gctctcatac tgattgtcgt catctatgcc ttctacatca ggaagggctt
     2641 cctcaaaaaa cgggctaaac aaacggggca accgacgagc ttttccgaca attttgccgg
     2701 gatctggaga atgctgactg cgtccaagcg gacctcg
//
LOCUS       DASSLD010000240         3039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1408248, whole genome shotgun sequence.
ACCESSION   DASSLD010000240 DASSLD010000000
VERSION     DASSLD010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408248"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VFK23_04385"
     CDS             complement(<1..992)
                     /locus_tag="VFK23_04385"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET7318357.1"
                     /translation="MPASLTPLPGLTVKGKKLRLPIIQGGMGVGVSLSPLASAVAREG
                     GVGIVSSACLDRLLSKRENKRFNTYEAVYREVSLAKSAGGFAGINIMVALVRDYEDSV
                     RGALDAGADVIISGAGLPLGLPAIQQPKDTALIPIVSSARALELICKKWERLHYRPDA
                     VVLEGPLAGGHLGFRMDQIDLEANKLENLLPPVKDTAMKYGDIPVIVAGGIYMHDDIV
                     RFLAMGADGVQMGTRFLATDESSASAAYKKAVLDAREEDILVAHKPGSPCGLPFRVIK
                     QSRMYVSALRRLRQPKCDKGYVLLKDADGNFTRCPAKESNEDCFCICNGLLSSAG"
     gene            complement(1045..2154)
                     /gene="had"
                     /locus_tag="VFK23_04390"
     CDS             complement(1045..2154)
                     /gene="had"
                     /locus_tag="VFK23_04390"
                     /EC_number="1.1.1.368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxycyclohex-1-ene-1-carbonyl-CoA
                     dehydrogenase"
                     /protein_id="HET7318358.1"
                     /translation="MSDDRKPDVIKSWQMTAEDSGQGGNPVRRLVRKDIPAPELKDGE
                     ALVEIAGCGVCGTDIGYFFDGIPTAAKPPLILGHEISGTVVAGEKSWIGREVIVPTII
                     PCRECEVCKKGRPNRCLRQKMPGNSYGVYGGFASHIPVPSRELCAVPRRGSFSLAELA
                     VVADAVATPYQAARRAGIGPGDRVVVMGVTGGLGIFLAQWAKFLGAETVVGVGRNARK
                     LEKALSFGVDFTINASGKSREDIKMEFFLECRRRKLDPKHSWKIFEMTGHRQAQEAAL
                     DLVGFAGMLVVVGYGAESIPCQLSRFMALDAELRGTWGCPPEHYPFILERVLSGDIRV
                     SELVETRPMSGIAATLEELKGQGSPDKRIVLIPDF"
     gene            complement(2151..>3039)
                     /locus_tag="VFK23_04395"
     CDS             complement(2151..>3039)
                     /locus_tag="VFK23_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HET7318359.1"
                     /translation="SDERIEGLRELTGIIHGRGGNIAIQISHGGFESRPELIGDTSFK
                     VPSVLEGMKRAKLEAEMSRSDIAQMVESFAGAAKRAKAADFDAVQIQASHGYLISQFL
                     SPLSNRRTDDYGGAVQNRARFLFEICESVRRAVGDAFPVLVKMNASDCVEGGLGVDDA
                     LWIMKELARMGVDAIEVSGGVASSADQGPSRKNIRAGQGEAYFASFAKTVREAVSPVP
                     VMAVGGIRSFETASNLLASGAADLVALSRPLICEPGLPNRWLGGDRAASACISCSRCF
                     FPGLHGKGIACQQKRRAQK"
BASE COUNT          547 a          997 c          876 g          619 t
ORIGIN      
        1 ccggccgaac tcaggaggcc gttgcagatg cagaaacagt cctcgttgct ttcctttgcg
       61 gggcagcggg taaaattgcc gtccgcatct ttcaaaagca catatccctt gtcgcatttg
      121 ggctgcctga ggcgccggag cgccgacacg tacatgcgtg actgtttgat cacccggaag
      181 ggaagtccgc agggagaacc cggcttgtgc gcgacaagga tatcctcttc tctagcgtcc
      241 aatacggcct tcttataggc ggccgatgcg ctgctctcat ccgtcgcgag gaatctcgtt
      301 cccatctgta cgccgtccgc tcccatggcg agaaatcgta caatgtcgtc gtgcatataa
      361 attcctcccg cgacgatcac ggggatgtcc ccgtacttca tcgccgtgtc ttttacggga
      421 ggcagcaggt tttcgagttt gttggcttcg agatcgatct ggtccatcct gaagccaaga
      481 tgcccgccgg ccaaaggacc ttcaagcacg acggcatcgg gccggtaatg tagcctctcc
      541 cacttcttgc agatgagttc gagggcccga gcggaagaga cgatcgggat cagtgccgta
      601 tccttcggct gttggatggc gggaaggccc aggggcaggc cggccccgga aataatgaca
      661 tccgctcccg cgtccagggc gccccgtacg gagtcctcat aatcccggac gagcgcaacc
      721 atgatgttta tgcccgcaaa acctccggca gactttgcaa gcgacacttc ccggtagact
      781 gcttcatacg tgttgaatct tttgttctcg cgtttcgaca ggagccgatc gaggcaggcg
      841 ctcgatacaa tgccgactcc cccttcccgg gcaacagcgc tggcgagggg ggagagcgac
      901 actccgacgc ccatgccgcc ctggatgatg ggcagtcgta attttttccc cttgacagta
      961 agacccggaa gcggcgttag cgaagctggc atgacggtcc tcttgatatg aatccgtgca
     1021 ttggtagtgt ttacactctt acccctaaaa atcgggaatt aatacgatcc ttttatccgg
     1081 gctgccctgt cctttaagct cttccaacgt agcagctatg ccgctcatcg gcctcgtctc
     1141 cacgagttcc gataccctga tgtctcctga cagcacccgc tccaggatga agggataatg
     1201 ttcgggagga cagccccaag tgcccctcag ctccgcgtca agggccatga acctcgagag
     1261 ctggcaggga atgctctcgg ctccgtagcc caccaccacg agcatccccg cgaaaccgac
     1321 caggtccaga gccgcctcct gggcctgccg gtggcccgtc atctcgaata tcttccagct
     1381 gtgtttgggg tcgagctttc tgcgtcggca ttcgaggaaa aactccatct tgatgtcctc
     1441 gcgcgacttc cccgaggcgt tgatggtaaa gtccacgccg aacgagagag ccttttccag
     1501 tttccgggcg ttccttccca cgcccacgac ggtctcggcg ccgaggaact tggcccactg
     1561 ggccaggaat atccccagtc cgccggtcac acccatcaca acgacccggt cgccggggcc
     1621 gatcccggct ctccgcgcgg cctggtaggg cgtcgccacg gcgtcggcga ccacggcaag
     1681 ctcggcgaga gagaagctcc cccttcgggg cacggcgcag agttccctcg acggtaccgg
     1741 tatgtgcgag gcgaagcctc cgtacacgcc gtagctgttg cccggcatct tctggcggag
     1801 acaccggttg ggcctgcctt ttttacagac ttcgcactcc ctgcaaggga tgatggtcgg
     1861 gacgatgact tcgcggccta tccacgactt ttcgcccgca accacggttc cgcttatctc
     1921 atgaccgagg atgagggggg gcttcgccgc cgtcggtatg ccgtcaaaaa agtacccgat
     1981 gtccgtgccg cagacgccgc agccggcaat ctcgaccagg gcctcaccgt ctttcagctc
     2041 cggcgcgggg atgtccttcc tcacgagcct ccggacgggg tttccgcctt gtccggagtc
     2101 ttccgccgtc atctgccatg acttgatcac atcgggcttg cgatcgtcgc tcacttctgg
     2161 gctctcctct tctgctggca tgctattcct ttgccgtgca ggcccgggaa aaagcagcgg
     2221 ctgcacgaaa tgcaggcaga ggctgcgcgg tcgccgccga gccaccggtt cggaaggccg
     2281 ggctcgcaga tgagcggtct ggacagggca acgaggtcgg cagcgcccga agccaggagg
     2341 ttacttgccg tctcgaagga tcgtatgcca cccaccgcca tcaccggaac gggggaaacc
     2401 gcttcccgca ccgtcttggc aaaggaggcg aagtaggcct ctccctggcc cgcgcggatg
     2461 tttttcctcg acggcccctg gtcggccgat gaggcgaccc cgccgctcac ctcgatggcg
     2521 tcgacgccca tccgcgccag ctctttcatg atccagagcg cgtcatcgac gccgaggccg
     2581 ccttccacgc agtccgaggc gttcatcttg accagcacgg ggaacgcatc gccgacggcg
     2641 cgcctcacgg actcgcagat ctcgaagaga aacctagccc ggttttgaac cgcgcctccg
     2701 tagtcgtcgg tcctcctgtt cgaaagcggc gacaggaact ggctgatgag atacccgtgg
     2761 ctcgcctgga tctggacggc gtcgaaatcc gccgccttgg ccctcttcgc ggcccccgcg
     2821 aacgattcca ccatctgcgc gatgtcggac ctcgacatct cggcttcgag tttcgcgcgt
     2881 ttcattccct cgaggaccga gggcactttg aagctcgtat cgccgatcag ttccggcctg
     2941 ctctcgaacc cgccgtggga tatctggata gcgatgttgc ctccccggcc gtggatgatg
     3001 ccggtcaatt cgcgcagccc ttctatccgc tcgtccgac
//
LOCUS       DASSLD010000241         3570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_908824,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000241 DASSLD010000000
VERSION     DASSLD010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908824"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VFK23_04400"
     CDS             <1..806
                     /locus_tag="VFK23_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7318360.1"
                     /translation="NDTKGRQSKPIMLLLETKNLTKHFGGLSALDDIDMRVERERIVS
                     IIGPNGAGKTTFFNCITGMYQPTKGTVIFHDRDVTGMTPHEITSLGVARTFQNIRLFG
                     GMSCLENVTVAAHCRTTAGVFGAIFRPEKVKREERDMVFRAMDILKFVGLENKHDALA
                     ANLSYGDQRRLEIARALGTEPHLLLLDEPAAGMNPQETQELMALIRKIRDNGVAILLI
                     EHDMKVVMGISDRVIVLDHGVKIAEGLPRDIQNDPAVIEAYLGKENVHA"
     gene            799..1500
                     /locus_tag="VFK23_04405"
     CDS             799..1500
                     /locus_tag="VFK23_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7318361.1"
                     /translation="MLELRDVIVSYGSIDALKGISMTVRKGEIVALIGANGAGKSTTL
                     MAASGIAALRSGAVFYEGKRISGLPAHEIVRMGISQVPEGRRIFGRMTVRENLEMGGF
                     SRNGKEIEKDIQRVLTMFPVLAERSGQMGGTLSGGEQQMLAIGRALMSRPSLLLLDEP
                     SLGLAPIIVSRIFAIIREINEQGTTVLLVEQNAKAALRLAHRAYVLETGKIVMQGEAS
                     QLEKDPGIKKAYLGE"
     gene            1574..2323
                     /gene="truA"
                     /locus_tag="VFK23_04410"
     CDS             1574..2323
                     /gene="truA"
                     /locus_tag="VFK23_04410"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET7318362.1"
                     /translation="MKNIKLILEYDGTNYHGWQSQAAPGRDIPTIQDTLERALKTLSG
                     EDVKTCSSGRTDAGVHALGHAANFKTASGIPPKAWAPALNHLLPRDIRVLESREVPPD
                     FHSRYSALAKTYRYRILNRRAPTALYRNHAWHVNLPLNVARMRRAVAFLVGKHDFSAF
                     RGSGCSAKTPVRTMKSALIRRTGEFIDISLEADAFLQYMVRNITGTLVEVGLGRFTPE
                     DVQRMLASRDRTLSGKTAPPQGLYLIDVSYP"
     gene            2629..3525
                     /locus_tag="VFK23_04415"
     CDS             2629..3525
                     /locus_tag="VFK23_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318363.1"
                     /translation="MRNWLNSVLLLLFTLVLVPRAGAAPDAATCLGCHGSMEGAVNVN
                     QDKYTKSVHGAFDCVQCHMVLKGAQHQGLSGKADKTTQDLAEAIAVKSKIDPIAQAAC
                     VQCHPDVYEAYKGSVHGKNVMEKKSPDGPVCTSCHGTPHYIQPKTSKDSAVNHFNVVK
                     ACGTCHEEKHMAEKYGFSPLVMERYLESFHGRKLKVGHAGAPSCVNCHGSHDIKSAKD
                     PASPVSGQNKIATCGKCHSGATPKFVAAITHKPLHPIAHFTELALIVLTMSVFAFICI
                     HVLLDIFADIREKLFRKGGKHE"
BASE COUNT          825 a         1005 c         1040 g          700 t
ORIGIN      
        1 ggaatgacac aaaaggacgg caatctaaac cgattatgct gttgctcgaa accaaaaacc
       61 tgaccaaaca cttcggcgga ctctccgcat tggacgacat cgacatgcgg gttgagcggg
      121 aacggatcgt gagcatcatc ggcccgaacg gcgccgggaa aacgactttt ttcaattgca
      181 tcaccggcat gtaccagccc acgaagggca cggtcatttt ccacgaccgg gacgtcaccg
      241 gcatgacccc gcacgagatc acgtcccttg gggtcgcgcg cacatttcag aacatccgtc
      301 tgttcggagg catgtcttgc ctggaaaacg tcacagtagc cgcccactgc cggacgacag
      361 ccggggtttt cggcgccata ttcaggccgg aaaaggtgaa gcgggaagag cgcgacatgg
      421 tgttccgggc catggacatc ctgaagtttg tcggactcga gaacaagcac gatgcactgg
      481 cggcgaacct gtcctacggc gaccagcggc ggctggagat agcgcgggcc ctcggcacgg
      541 aaccgcacct gctgcttctc gacgagccgg ccgcgggcat gaatcctcag gagacgcagg
      601 aactcatggc gctgatccgg aagatccggg acaacggcgt tgcgattctc ctgatcgagc
      661 acgatatgaa ggtcgtcatg ggcatctcgg accgggtcat cgtgctggac catggcgtca
      721 agatcgccga ggggctgccc cgggacatcc agaacgaccc tgccgtgatc gaggcctatc
      781 tcggcaagga gaacgtccat gcttgaattg cgcgacgtca tcgtgtccta tggcagcatc
      841 gacgcgctca aggggatcag catgacggtc cggaagggcg agatcgtcgc cctgatcgga
      901 gcgaacggcg ccggcaaatc cacgaccctg atggccgcct ccggcattgc ggcgcttcgc
      961 agcggagcgg tcttctatga aggaaaacgg atttcaggcc ttcccgcaca cgagattgtc
     1021 cggatgggga tctcgcaggt gcctgaaggg aggcggatat tcggacgcat gacggtccgg
     1081 gaaaacctgg agatgggcgg tttctcccgg aacgggaagg agattgagaa ggacattcag
     1141 cgcgtgctta cgatgtttcc ggttctcgcc gagaggtccg ggcagatggg cggcacgctt
     1201 tctggcgggg aacagcagat gctcgccatc ggcagggcac tcatgtcacg gccgagcctg
     1261 cttctcctcg acgagccttc cctcggactg gcgcccatca tcgtgagcag gatattcgcc
     1321 atcatcaggg agatcaacga gcagggaacg accgtgctcc tcgtcgaaca gaacgcaaag
     1381 gcggccctcc ggctcgcgca ccgggcctac gtattggaga ccggcaagat cgtgatgcag
     1441 ggggaagcct cgcagctcga gaaagacccg ggaatcaaga aagcatatct tggagagtag
     1501 ccaggagtca gaatccagaa gcagcataac agcatttctc ctgactcctg tcttctggct
     1561 cccgacttct gcaatgaaaa acatcaaact catccttgaa tacgacggca cgaattacca
     1621 cggctggcag tcacaggcgg cgcccggacg ggacatcccg acgattcagg atacgctcga
     1681 gcgggcgctc aagaccttgt ccggggaaga cgtgaagacc tgttcatcgg gaagaacgga
     1741 tgccggcgta cacgcgctcg gccacgcggc caatttcaag acagcgagcg gaattccgcc
     1801 gaaggcctgg gcgccggcgt tgaatcatct tcttccccgc gacatccggg ttctggagtc
     1861 ccgtgaagtc cccccggatt ttcactcccg gtacagcgcg ctcgcgaaga cgtaccggta
     1921 tcgtatttta aacagacggg ctccaacagc actgtatcgg aatcatgcat ggcacgtcaa
     1981 tcttccgttg aacgtcgcgc gtatgagaag ggcggtcgct tttcttgtcg ggaaacatga
     2041 tttctcggcc tttcgcgggt cgggctgcag cgcaaaaacg ccggtccgaa cgatgaaatc
     2101 agccctgatc aggagaaccg gggaattcat cgatatatcg cttgaggcgg acgctttttt
     2161 gcagtacatg gtgcgaaata tcacgggaac tcttgttgag gtcggtctgg gcagattcac
     2221 tccggaggac gttcagcgca tgcttgcgtc gcgcgaccgg accctgtccg gaaagactgc
     2281 cccgccgcag ggactttacc tgatagatgt ttcttatccg tgaaaaatat caaccactgt
     2341 ttgtttgcaa atgtgacaga aatacacata aattctcaat tatgcaaaaa tcggtgaggc
     2401 ttcatccatt gataaatcaa cgtgttattc gtggcacgta acgtgcaaaa gatggtcacc
     2461 ggatttgtgc tgcgtcctgg gtgaatttaa gcttttcatc ttcatgaaat tctgatatcc
     2521 tatcaaagtt ttgtgttccg gtttgtacgg gtcaggtcaa ttacgctgca agcaggtccg
     2581 cttatctcta cacattttac tttacacgat agcgcgaaag gagaacgtat gaggaattgg
     2641 ctgaactctg tactgctgtt gctgttcacc ctggtcctgg ttccccgggc tggcgcagcg
     2701 cccgacgccg ccacatgcct cggctgtcac ggatcgatgg aaggggccgt caatgtgaac
     2761 caggacaaat acaccaaatc ggttcatggc gctttcgatt gcgtccagtg ccacatggtc
     2821 ctgaaagggg cgcagcacca gggactttcg ggcaaggccg acaagaccac gcaggatctg
     2881 gccgaggcaa tcgctgtgaa aagcaagatc gatcccattg cccaggcggc atgcgtgcag
     2941 tgccatcccg acgtctacga ggcctacaag ggaagcgtgc acggaaagaa cgtcatggag
     3001 aagaagtccc cggacggccc ggtctgcacg agctgccacg gcacgccgca ttatatccag
     3061 ccgaagacca gcaaggactc ggcggtcaat catttcaacg tcgtcaaagc atgcgggacc
     3121 tgccatgaag agaagcacat ggcggagaaa tacggattct cgccgctcgt catggaacgc
     3181 tatctggaga gcttccacgg aaggaagctc aaggttggcc atgcgggcgc gccgtcgtgt
     3241 gtgaactgcc atggttctca tgacatcaaa agtgccaagg accccgcttc acccgtctcc
     3301 ggccagaaca agatagcgac ctgcggaaaa tgccacagcg gggcgacgcc caagtttgtc
     3361 gccgcgatca cccataaacc gctgcacccc atcgcccatt ttaccgagct tgcgctcatt
     3421 gtccttacga tgagcgtgtt cgcgttcatc tgcatccacg ttcttttgga tattttcgcg
     3481 gacatccgcg agaagctttt cagaaagggg ggcaagcatg aataacgaca tcccgaagga
     3541 aatcgagaga ttcgacctct ccgcgaggat
//
LOCUS       DASSLD010000242         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1310364, whole genome shotgun sequence.
ACCESSION   DASSLD010000242 DASSLD010000000
VERSION     DASSLD010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310364"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VFK23_04420"
     CDS             complement(<1..286)
                     /locus_tag="VFK23_04420"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HET7318364.1"
                     /translation="MTPRELLITGAKELGIDLSVEQVNSLFIYLAELKKWNRKINLTA
                     IRDERDVIIKHVLDSLSYIRGFGQTERVRLLDMGSGAGFPALPIKVVRPGI"
     gene            complement(362..2239)
                     /gene="mnmG"
                     /locus_tag="VFK23_04425"
     CDS             complement(362..2239)
                     /gene="mnmG"
                     /locus_tag="VFK23_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET7318365.1"
                     /translation="MSQQKKYDVIVIGAGHAGCEAALAASHMGCAALMLTMNLDSIAL
                     MSCNPAIGGLAKGHLVKEIDALGGEMAKIIDRTGIQFRVLNRSKGPAVRGTRAQADKQ
                     RYRLAMKESVERQSGLDVKQGLVERIIVEDGRVVGVETNIGVQYTAKAVIIATGTFLK
                     GLIHIGLVHYPSGRAGEFPSVGLSDSLSEMGFEIGRLKTGTPARLNGRTIDFSKMKSQ
                     PGDDPAPFFSLTEKSHPLPQMPCYLTYTNINTHKIILNNLHHSPLYAGVIKGIGPRYC
                     PSIEDKVKRFSDRERHQVFLEPEGLDTEEYYANGVSTSLPYEVQTEMYRTIPGLEDVE
                     IMRPAYAIEYDFAPPTQIRHTLETKRVAGLFFAGQINGTSGYEEAAAQGLMAGINAAL
                     TIQKRPPLVLSRSEAYIGVMIDDLVTRGTAEPYRMFTSRAEYRLILREDNADMRLRQK
                     GWEIGLVSEENYHMFTQKHEDIEREVGRLKKTWIKPDPQVNDILSERGSAELSADASL
                     DQLLKRPEIEYKDIIEISPPPEFLTPVAAEQVEIQSKYEGYIQRQLQQVERFSKLEQK
                     QIPADIDYDAITGLGSEVKQKLNQVRPVSLGQASRISGVTPAAISLLMVALEKRKRGR
                     I"
     gene            complement(2438..>2833)
                     /locus_tag="VFK23_04430"
     CDS             complement(2438..>2833)
                     /locus_tag="VFK23_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET7318366.1"
                     /translation="ETRGRNTVAVINKSDLPRKLEPLEWPGVQISLSCKSGEGIDDLR
                     KAVAEPVMKGAAGQREHPWAVNRRHRTALEQARGSLEKALESIGADLSPEFVALDLRE
                     ALDHLGLIIGATYTDDILDRIFNDFCIGK"
BASE COUNT          595 a          808 c          685 g          745 t
ORIGIN      
        1 agattcccgg cctcacaacc ttgattggaa gcgccgggaa accggcgccg gaccccatgt
       61 caagaagcct cacccgctcc gtctgaccga atccccggat gtaggaaagg gaatcaagca
      121 catgctttat gatcacgtcc cgctcatccc ttatggctgt caggttgatc ttcctgttcc
      181 acttcttgag ttctgccaga tagatgaaaa gcgaattaac ttgttcgacc gaaagatcga
      241 tgccgagctc ttttgcgccc gttatcaata actcccgcgg cgtcatgagt caccgctcgg
      301 cctatgacgc gtttcatccc tgcccaaact tcctcctccg tattccgaat tccccattct
      361 cctatatcct tcccctcttc cttttttcca acgccaccat gagaagagag attgcggccg
      421 gagtcacgcc cgatatcctc gatgcctgcc ccagcgatac cggccggacc tggttcagtt
      481 tttgtttcac ctcgctgcca aggccggtta tagcgtcgta gtcaatatca gcgggtattt
      541 gtttctgctc cagttttgag aagcgttcca cctgctggag ttggcgctgg atataccctt
      601 cgtacttcga ctggatctcg acctgctccg cagcgaccgg ggtaagaaat tccggcggcg
      661 ggctgatctc aatgatatct ttatattcga tctccggtct ctttaatagc tggtctagcg
      721 atgcatcagc ggacagctcg gctgatcccc gctccgagag gatatcattg acctgcgggt
      781 caggctttat ccatgttttt tttagtcttc ccacttccct ctcaatatcc tcgtgttttt
      841 gggtaaacat atgataattt tcttcagaaa cgagccctat ttcccaccct ttttgcctca
      901 atcgcatatc cgcattgtct tccctgagga taagccggta ttcggcacgc gatgtgaaca
      961 tacggtacgg ttccgcagtg cccctggtaa ccaggtcatc aatcatgaca cctatatagg
     1021 cttcggagcg ggaaagcacg agaggcggtc ttttttgtat cgtgagcgcg gcattgattc
     1081 ctgccataag cccttgggcg gcagcttctt catacccgga cgtgccgttt atctggccgg
     1141 cgaaaaaaag acctgccacc ctcttcgtct cgagagtgtg cctaatctgc gtggggggcg
     1201 caaaatcata ctcgatggcg taggcaggcc tcatgatctc gacatcttcg agccccggga
     1261 tggtccggta catttccgtc tggacttcgt aaggaagact cgttgagacg ccgttcgcgt
     1321 aatattcttc cgtatcgaga ccttccggtt cgagaaatac ctggtgccgc tcgcggtccg
     1381 agaatctctt gaccttatct tctatcgacg gacaataccg tggcccgata cccttgataa
     1441 caccagcata aagtggggaa tggtgtagat tattcaatat tattttatgg gtatttatat
     1501 ttgtgtaggt taaataacag ggcatttgtg ggagaggatg gcttttttcc gttaaagaga
     1561 aaaaaggggc aggatcatct cctggctgag atttcatttt agagaagtca attgttcttc
     1621 cgttgagtct ggccggagtg cctgttttta gtcttcctat ttcaaaaccc atttcactca
     1681 agctatcgga aagtccgaca gacgggaact cccccgccct gcctgaggga taatgcacaa
     1741 gcccgatgtg aatgagacct ttcaaaaaag tcccggtggc gatgatcacc gcttttgcgg
     1801 tatactgaac gccaatgttc gtctccacgc ccacgaccct gccgtcttcg acgatgatcc
     1861 tttccacaag cccctgcttc acgtcaagtc ctgattgacg ctctaccgat tctttcatgg
     1921 cgagcctata gcgctgcttg tcagcctggg cacgcgttcc gcgcacggca ggacctttgc
     1981 tccggttcag aacccggaat tggatacccg tgcggtctat aatcttcgcc atctctccac
     2041 ccagggcgtc gatctctttc accaggtgcc ccttggcgag gccaccgata gcaggattgc
     2101 aggacatgag agcaatgctg tccagattca tggtgagcat gagcgcggcg cagcccatgt
     2161 gggaggctgc aagcgccgcc tcgcagcccg cgtgccccgc gccgatcaca atgacgtcgt
     2221 atttcttttg ttgtgacatt ctaatttgac tccgaaacag agcaatcgcg gagacaggaa
     2281 aaaatgaagc actaaattcc aagctcgaaa cgctatacaa tatcaaatct caacaaattt
     2341 gatcatcagt gttttttagc atgtttcgga ttgagaactt cgtgcctggg atttcctcta
     2401 ctccataccc caactgaact attcgtattt taatttatta ctttccgatg cagaaatcgt
     2461 tgaatatccg gtcgagaatg tcgtccgtat acgtcgctcc gatgatgagg cccagatgat
     2521 ccagggcctc gcgaaggtcc agggcgacaa attcgggaga tagatcggcc cctatggact
     2581 caagcgcctt ttccagactg cccctcgcct gctccagcgc ggtccggtga cgccggttca
     2641 cggcccaggg gtgttcccgc tgacccgcgg cccccttcat gaccggttcg gcaacggcct
     2701 tcctgagatc gtcgatgccc tccccgcttt tgcacgacag ggatatctgg actcccggcc
     2761 actccagcgg ttcgagtttt ctcggtaggt ccgatttgtt gatgaccgcg accgtgttcc
     2821 tgccccgcgt ttc
//
LOCUS       DASSLD010000243         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1245199, whole genome shotgun sequence.
ACCESSION   DASSLD010000243 DASSLD010000000
VERSION     DASSLD010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245199"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..718
                     /locus_tag="VFK23_04435"
     CDS             278..718
                     /locus_tag="VFK23_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7318367.1"
                     /translation="MRTFVFGLILSLALVPLGGSYSGASENHARHGGSGTNPLIEEMV
                     ILDSVFCKVVSAVALGDGARVHKALESMHGAMEKTHEGVHAGTVTLPRNNDRMKEFVN
                     LDLKFHEKLEALAEAGERNDQEKMLALTKELIGRCVECHRTFRR"
     gene            777..1790
                     /gene="dusB"
                     /locus_tag="VFK23_04440"
     CDS             777..1790
                     /gene="dusB"
                     /locus_tag="VFK23_04440"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET7318368.1"
                     /translation="MLLNRILGNNPLVLAPMAGITDLPFRLICKEMGAGLVFSEMVSV
                     EALIREHRRTHGMLRTDPAERPVVFQIFGSRPASMAEAAHIVSQGEVDFIDINMGCPV
                     PKVLKSGAGSALLRDLELAKEIMSAVVKASNVPVTVKIRLGWDSKNIVAVDLARAAED
                     AGVAAVTIHGRTKAQGFSGTADWNMIRSVKESVSIPVIGNGDVRSAGDVRRMLDETGC
                     DGVMIGRAIQGNPWIFRDAKRYLETGVVPAHPSYDERQAVMLRHLNDMIRLVGENSGV
                     REMRKHLCWYTKGLPGGSEFRQRINLLERMTDVRNAISSYFHALSTTSNLLCHSRESG
                     NPF"
     gene            1851..2246
                     /locus_tag="VFK23_04445"
     CDS             1851..2246
                     /locus_tag="VFK23_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003317410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HET7318369.1"
                     /translation="MVLSNKRSENDDANRLIAETRRDRELREKDYRERALRMYPHICA
                     RCGREFSGKRLRELTVHHKDHNHDNNPPDGSNWELLCLYCHDNEHQKYLDEQSFSKTT
                     PGGDTEPRSTYKPFAELGNLLKGKKNPIE"
     gene            complement(2369..>2598)
                     /locus_tag="VFK23_04450"
     CDS             complement(2369..>2598)
                     /locus_tag="VFK23_04450"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HET7318370.1"
                     /translation="YKDEFVRHKILDLLGDMFLVGMPIIGHIVAHKSGHGLNARMAAK
                     LLASPESWVLVGSPVRLEVKHQEPQLLQAAL"
BASE COUNT          627 a          707 c          727 g          537 t
ORIGIN      
        1 attattgtca aggattttct cataaaacta aacatattct gccctttcgg tttattttac
       61 aagagaaaaa tcgtcgcata ccttgttaaa tgatttacaa accaattgac taagactaaa
      121 gctgcattaa ccccttattg atttgcattc atctcgggga gggttttcac tctaacccac
      181 tgccagggtc tccctccttc gagtataatt aaaaaaatcc ttattgtaac cgacaattga
      241 gaacccagcc gtttcgtatc catggtgagg aggaaacatg agaaccttcg tctttggttt
      301 gatcctgtct ctggcgctcg tccccttggg aggaagctac tctggagctt ctgaaaatca
      361 tgcccgccac ggcggatcag gaacaaatcc ccttatcgag gaaatggtca tcctcgacag
      421 cgtcttctgc aaggtcgttt ccgcagttgc gctcggggat ggagcgcggg tgcacaaggc
      481 gctcgagtcc atgcacgggg ccatggaaaa aacccatgaa ggggtccatg ccggaacggt
      541 aacgcttccc agaaacaatg accgcatgaa ggaattcgtt aacctggacc tgaagttcca
      601 tgagaaactg gaagctctgg ccgaggccgg agagcgcaac gatcaggaga aaatgcttgc
      661 actcacgaag gaacttatcg gccgctgcgt cgaatgtcac cggacgttca ggcggtaagg
      721 gcgtacctgc ggcgaaagat tcttgttttc ccacgcattt cggttataat ggctccatgc
      781 ttctcaaccg catactcgga aacaatcccc ttgtcctcgc gcccatggcc ggtattacgg
      841 acctcccctt ccgtctgatc tgcaaggaaa tgggcgcggg cctcgtattt tcggaaatgg
      901 tgagcgtcga agcgttgatc cgggagcaca ggcgcacgca tgggatgctc aggacagacc
      961 ctgccgagcg cccggtggta tttcagatct tcggttcccg gcccgcatcc atggccgagg
     1021 ctgcgcacat tgtgtcgcag ggagaggtgg atttcatcga catcaacatg ggttgtcccg
     1081 tcccgaaggt cctgaaaagc ggcgcgggct cggcgcttct gcgggacctc gaactggcga
     1141 aagaaatcat gtctgcggtc gtgaaggcct cgaacgtgcc ggtgaccgtg aagatccgcc
     1201 tgggttggga ctcaaagaac atcgtggccg tggatcttgc cagggcagct gaagacgccg
     1261 gtgtcgcagc agtgaccatc cacggcagga ccaaggcaca gggcttctcg ggaaccgctg
     1321 actggaacat gatcagatcc gtcaaggaat ccgtttccat tcccgtgatc ggcaacggcg
     1381 atgttcgctc ggccggggac gtcagaagaa tgctggacga aaccggctgc gacggcgtca
     1441 tgatcggcag ggcgatccag gggaatccct ggatattccg cgatgcaaag cggtatcttg
     1501 agacaggcgt cgttcccgcg catccctctt atgatgaacg gcaagcggtc atgctccgcc
     1561 atttgaacga catgatcaga ctcgtcggcg agaatagcgg cgtgcgcgaa atgcgcaagc
     1621 acctgtgctg gtatacgaaa ggcctgcctg gagggtcgga gttcaggcag agaatcaatc
     1681 tccttgagcg catgacggat gtgagaaacg ccatttcaag ttattttcat gccctcagta
     1741 caacgtcgaa tctcttatgt cattcccgcg aaagcgggaa cccattttga agataattcg
     1801 ataaaagccg atggatacct gcgttcgcag gtatgacagc taaaatacgc atggtattga
     1861 gcaacaaacg atcagaaaac gacgacgcca atcggcttat cgccgagacc cggcgcgacc
     1921 gcgagctccg ggaaaaggac taccgggagc gggcgctcag gatgtatccg cacatctgcg
     1981 cgcggtgcgg acgggaattc tcgggcaaaa ggcttcgcga gctcacggtc caccacaagg
     2041 accataatca cgacaacaac ccgcccgacg gcagcaactg ggaacttctc tgcctgtact
     2101 gccacgacaa cgagcatcag aaatatctgg acgaacagtc attcagtaaa acgacgccgg
     2161 gcggcgatac ggagccgcgt tccacgtata aaccgtttgc cgagctgggg aacttgctga
     2221 agggcaaaaa aaatccgatc gaataggatg gcatttttca caacgggact gccggatcac
     2281 aagcccaggc ccggacgatc gcataaaaca aaagagccgg aggcaccaat gtgctccggc
     2341 tccggttctt tgcgggatac cgtctcggtc acagtgcggc ctgcaacaac tgcggctcct
     2401 gatgtttgac ttccagcctg acgggcgaac cgaccaggac ccagctctcc gggctcgcga
     2461 ggagcttggc cgccatccgc gcgttcaggc cgtgtccgga cttatgggcc acgatgtgtc
     2521 cgattatggg catgccgaca aggaacatgt cgccgagaag atcgaggatc ttgtgtcgga
     2581 cgaattcatc cttgtacc
//
LOCUS       DASSLD010000244         2571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_195659,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000244 DASSLD010000000
VERSION     DASSLD010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195659"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1863)
                     /locus_tag="VFK23_04455"
                     /pseudo
     CDS             complement(76..1863)
                     /locus_tag="VFK23_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223229.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
     gene            complement(1962..>2571)
                     /gene="rpe"
                     /locus_tag="VFK23_04460"
     CDS             complement(1962..>2571)
                     /gene="rpe"
                     /locus_tag="VFK23_04460"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET7318371.1"
                     /translation="RLGEEIKAVEAAGADLIHVDVMDGHFVPNITIGPLIVEAARRST
                     KLLLDVHLMITNPELYIADFAKAGADYITVHCETAFHLNRLVQSIREHKGLKAAVSLN
                     PATPLTALDYILSELDMVLVMSVNPGFGGQAFIPSALDKIAKLRKRIDELGLHTEIEV
                     DGGVKPENAASVIAAGADILVAGSAVFGKKDYAAAIRGIRGE"
BASE COUNT          446 a          768 c          770 g          587 t
ORIGIN      
        1 cgtctccgca gcaaaaaaag cggcggtgga aaagtttccc accgccgctt tttcgttttc
       61 ctgtatgcta caacgctact ccgcccggac caggtcctcg aacctgatgt ccacgccgaa
      121 cacgccgatg atgtcgctct cttcgttgct gatgggcgcc gacacggtga tgcagagcgc
      181 gccggtgatc ttcgaggtgt agaagtccga cacgaagctc ctgccgtcct tcatggggct
      241 gatgaaccag ggccggtcgg agaaattgtc gcccagcccc gagtgttcga actgcgcctt
      301 gtactggatc tgggtgatgt tcttcgtggt tttgttccct tcacggttca ccacgtaggc
      361 gaactggatg aagggattgt cctcgagaag cttctggatg actacctcct gcttcgcatg
      421 gtccatggag cgcatttcct tgcgctccac catgccctcg accaggtgca gggcgatctc
      481 ctgcgccttc tttttgagct tgtccaggtc cgagacgaag tattcgggaa gatacttctt
      541 cgccttctcc aggagttcgt tgtgcgagat gctcgtggtt ctgccctgct cgtactgctc
      601 catcacccat ttgtttatct tggccaggcc ggggtgcctt ttgtcgatct tcttttccgc
      661 atcgaggtcc aggtggctgt tcacccaatg agcgatgccg gcgacaccgg acttgtcggt
      721 gatgatgacg cccatgggcc gcttgaggat gttcgtcgtg tcgaagatgt tatagatttc
      781 ctcgttcttc atcatgccgt cggcatggat gccggcggag gtcgagttga actcggcgcc
      841 tacgaagggg tagttggagg ggatggcaaa gcccagttcg ctccggaaat actcggccat
      901 ctcggtgatc gccgtggtgt cgatgcccgc gatgttgccg gtgaggccga tgtaatcgat
      961 gagcatggcc tcaaccggcg tgttgcccgt ccgctcgccg aaccccagga gagcgccgtt
     1021 caccgccgaa catccgtaca tccaggccgc cgtcccgttg acgaggccct tgtgaaagtc
     1081 gttgtggccg tgccattcga gctgctccga aggcacaccg ccgtcgtgta tcagggccta
     1141 gaatatcccc gggacgcccc gcggcagggc cgtgccgggg aacgggatgc cgtagcccat
     1201 ggtgtcgcag gccctgatct tgaccgggat cttcgactcc tccgagagcc tcatgagctc
     1261 ctgcgcaaag gggatgacga agccgtagaa gtccgcccgg gtgatgtcct cgaggtggca
     1321 gcgcggggtg atgccctgct cgagcgccgt cgtgacgatg tccaggtact gctggagggc
     1381 gtccttgcgc gtcatgttaa gcttcaggaa aatatggtag tccgagcagg acgtgaggat
     1441 gcctgtctcc ttgagaccca tgtccttcac cagcttgaag tccttcttgt tcgcgcggat
     1501 ccagcccgtg atctcaggga agcggtatcc gcgctccctg cagagcctga ccgcctcgcg
     1561 gtctttttcc gaatacagga agaactcggt ctggcggatg acgccgctgg gcccgctcat
     1621 gcggtgcagg aaatcgaaga tgtcggcgat ctgctggggc gtatacggcg gccgggactg
     1681 ctgcccgtca cggaaggtgg tgtccgtgat ccagatgtcc ttcgggatat tggtctggat
     1741 gagccggtcc tcgagcacca cccggggcac gctcgtatag gggaacatgt cgcggtacag
     1801 ttccggcccg ctcacgttct tgagctcgca agggactttc attctctgtt ttttgatggt
     1861 catgggaaac tcctgataga aaatcaaatt caaagctcaa aactcaggcc atgtgccgtt
     1921 tcgatacttt gaactttgta ctgtgaactt tgaacttttt gctactctcc ccttatcccc
     1981 cgtatcgccg ccgcatagtc cttcttgccg aaaaccgcag agcccgctac caggatgtcc
     2041 gcaccggccg cgatgaccga cgccgcgttc tcgggtttca ctccgccgtc cacctcgatc
     2101 tcggtgtgaa gacccagttc gtcgatccgt ttccggagtt tcgcgatctt gtccagcgcc
     2161 gaggggatga aggcctggcc tccgaacccc gggttcaccg acatgacgag caccatgtcg
     2221 agctcggaga gaatatagtc cagcgccgtg agcggcgttg ccgggttgag cgatacggcc
     2281 gccttcaggc ccttatgctc cctgatggac tgcacgagac ggttcaggtg gaacgccgtc
     2341 tcgcagtgca cggtaatgta gtcggccccc gccttggcga aatcggcgat gtacagctcg
     2401 gggttcgtga tcatgaggtg cacgtccagc aggagcttcg tgctcctacg ggcggcctcc
     2461 acgatgagcg gcccgatggt gatgttcggc acgaaatgcc cgtccatcac gtcgacatgg
     2521 atcaggtccg cgcccgcggc ctcgaccgcc tttatctcct cgcccagacg g
//
LOCUS       DASSLD010000245         2106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_39489,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000245 DASSLD010000000
VERSION     DASSLD010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39489"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VFK23_04465"
     CDS             complement(<1..894)
                     /locus_tag="VFK23_04465"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HET7318372.1"
                     /translation="MAKKPPARKKGKGGGDRWIVGGIAICLVVIALALWGPLRKFRAP
                     AEKRTAPHAKQEEPAVIAKPPASKKSPAKDTPTRDGGAGPALLGSAAPGKTVMVAIVI
                     DDLGRDIKQAKEILSLPANVTFAVMPGLTQSQKIAELARNNNREILLHLPMEYRSKNG
                     TPAPGMLRSDMTPMELLNTVSDDVASVPGAVGINNHEGSALTENKEAMKFLMAELKAR
                     NLMFLDSLTSPKSVAYQTAREFGLKSARRDVFLDNDSNNPASIRKQLDELVEIARKNG
                     KAIGIGHPHPATVIELRKWLAD"
     gene            1073..2101
                     /locus_tag="VFK23_04470"
     CDS             1073..2101
                     /locus_tag="VFK23_04470"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008427989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET7318373.1"
                     /translation="MGFTNVRQIPKAKDIIEKMPLSAELKKVKKEKDKEIQGVFTGAV
                     DKLLRIIGPCSAHDEDAVCDYISRLAKVQDEVKERIVVIPRIYTNKPRTTGIGYKGMA
                     HQPNPKQEPNIVEGIKAIRKMHIRALRESHLPAADEMLYPNNYADLEDVLSYVAVGAR
                     SVENQQHRLTCSGLDVPVGMKNPTSGDFDVMFNAVQASQAPHVFSYNGWEVKTSGNPL
                     THCVLRGAMNQFGQSIPNYHYEDLIRVAKTYKERAFANPVIIVDTNHANSNKQYKDQP
                     RIAKEVMRTREHSELVRKMVKGFMIESFIEEGSQKVSENVYGKSITDPCLGWKDTERL
                     IKQLAELV"
BASE COUNT          436 a          611 c          633 g          426 t
ORIGIN      
        1 atccgcgagc cactttctga gttcgatgac ggttgcgggg tgaggatggc cgatgccgat
       61 cgccttgccg tttttccggg cgatttcaac cagctcgtcg agctgtttcc gaatggaggc
      121 gggattgttg ctgtcgttgt ccaggaacac gtcgcgcctt gcggacttga ggccgaactc
      181 ccgcgcagtc tggtaggcca cgctctttgg gctggtgaga ctgtcaagga acataaggtt
      241 ccgcgccttc agctccgcca tcaggaactt catggcttcc ttgttctccg taagcgctga
      301 gccctcgtgg ttgttgatgc ccacggcgcc ggggaccgac gcaacatcgt cgctcaccgt
      361 gttcagtagt tccatcggcg tcatatccga acgcagcatg cccggcgccg gcgtcccgtt
      421 cttgctccgg tactccatgg gcaggtggag caggatctcc cggttgttat tccgcgccag
      481 ttcggcgatt ttttgggact gggtcagccc cggcatgact gcaaaggtaa cgttggcggg
      541 tagtgagagg atttccttcg cctgctttat gtcccggccc aggtcgtcga tgacgatggc
      601 aaccatgact gttttcccgg gcgccgcgct tccgagcagc gccggccccg cccctccatc
      661 gcgcgtaggc gtatcttttg ccggtgattt tttggatgcg ggaggcttgg cgatgactgc
      721 cggctcctcc tgctttgcgt ggggcgccgt ccttttttcg gccggggcgc ggaacttgcg
      781 caggggcccc cacagggcga gcgcgatcac cacaagacag atggcgatcc cccctacgat
      841 ccagcgatcg ccgccgcctt tccccttttt ccttgccgga ggtttctttg ccatggcaag
      901 ctttttatac aatattacgg aagaaaaggc cagagttttt tgatccgcgg agggcggtcc
      961 cgctctttgg cgcgttacgg acctcttttt ggcttgactt agccctcaac ggattttata
     1021 ctgcccctga cgcacgcggg accgtggtgc gaagagagga gtacactgga atatgggctt
     1081 cacgaatgtc cggcagatcc cgaaggccaa agacatcatc gaaaaaatgc cgctttcagc
     1141 ggaactgaaa aaggtcaaaa aagagaagga caaggaaatt cagggcgtgt tcaccggcgc
     1201 cgtggacaag ctccttcgca tcatcggacc ctgctcggcc cacgacgagg acgccgtgtg
     1261 cgattatatc tcgcgtctcg cgaaggtgca ggatgaggtg aaggaaagga tcgtcgtcat
     1321 cccccgcatc tatacgaaca aaccccgcac aaccggcatc ggctacaagg gcatggcaca
     1381 ccagccgaat ccgaagcagg agccgaacat cgtggagggg atcaaggcca tccggaagat
     1441 gcacatccgg gcgctcaggg agtcccatct cccggccgcc gatgagatgc tctatcccaa
     1501 caactacgcg gacctggaag acgtgctctc ctacgtggcg gtcggcgcgc gctcggtgga
     1561 aaaccagcag caccgcctga cctgcagcgg cctcgacgtc ccggtgggca tgaagaaccc
     1621 gacgtcagga gatttcgatg tgatgttcaa cgcggtccag gcgtcccagg cgccgcatgt
     1681 gttcagctac aacgggtggg aggtcaagac ctcaggaaat ccgctgaccc actgtgtgct
     1741 ccggggagcg atgaaccagt tcggccagag tatccccaac taccattacg aggacctcat
     1801 cagggtagcc aagacgtaca aggaacgcgc cttcgccaat cccgtgatca tcgtggacac
     1861 gaaccacgcc aactcgaaca agcagtacaa ggatcagccg cgaatcgcaa aagaggttat
     1921 gcggacacgt gagcattccg aactggtgcg caagatggtg aaaggcttca tgatcgaaag
     1981 cttcatcgag gagggcagcc agaaggtgtc ggaaaacgtg tacggcaagt ccattaccga
     2041 cccgtgcctg gggtggaagg acacggagcg gctgatcaaa cagttggcgg agttggtata
     2101 agtcaa
//
LOCUS       DASSLD010000246         4446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_36872,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000246 DASSLD010000000
VERSION     DASSLD010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36872"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFK23_04475"
     CDS             <1..189
                     /locus_tag="VFK23_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318374.1"
                     /translation="SNVTSGLPKDLKSIVASAYLSYLAGAGLSAAMVNVNDAEIMKVV
                     YLIKTFRDEITFAAGDLA"
     gene            complement(282..1286)
                     /locus_tag="VFK23_04480"
     CDS             complement(282..1286)
                     /locus_tag="VFK23_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET7318375.1"
                     /translation="MKLPRTYLRPLRYAVQFGFLLLTLYIGWRFYEFVLHFEAGSPFV
                     ERPASVDAFLPIGGLMAFKYFILTGTVDPVHPAGFVLFAAILGVSLVMKKGFCGWICP
                     VGTLSQYFWMVGEKIFGRNFRITGFSDTSLRSIKYVLMGFFLLAIGVMPMWSMAMFFI
                     GDYYKTVDVRMMMFFTEMSTVTMWVLIALAALSFLYKNVWCRYLCPYGALLGLVSRLS
                     PFKVRRNEEKCGHCHACTRHCPTHIDVENTAVVKSAECYGCLTCVSRCPSEGALNLTV
                     STGKKVGIVKAWLFPVVLVALFYLVVGIGMAAGKWHSKVPYEDYKELIPEVQKEYSKR
                     "
     gene            1545..2936
                     /locus_tag="VFK23_04485"
     CDS             1545..2936
                     /locus_tag="VFK23_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET7318376.1"
                     /translation="MSNDLDGFFHPKTVALIGATDNPLKPGAAILENLSHYRGAVFPV
                     NPKHQTLLGRRCYPSVASVPDEIDLAIVALPAGLVEQELARLHDKGISRAIVISSGFA
                     EAGGPGIDLQKRLSETAKSFGIRIIGPNALGIFNTDNGFDSFFVSRHRVSRPAAGRLS
                     IISQSGAITVILMEAFARDDIGIAKAVNYGNRLDIDDADCLDYFADDPQTGAVAMYME
                     SVAEGRKFLKSAKAFVAKKPLIVWKAGKHELGASAVASHTATLAGRYGLYQAAFRQAG
                     AVEVQGFDHMIDAAKAVALMEHPCFGNRLLIVTNGGGMAVAASDQAQQEGFSMPPVPE
                     QVRKRLDAALPLFFGKNNPIDLTGSGSNEDYYTALTEALPHYDAAVIIVLMGSTTVTD
                     QATELIARACRDAKKPVTCCILQGLGYTREAMNTLLRHGIPVYPSPERAVRALAVLRK
                     ATHPEIKIEGAKR"
     gene            3054..3770
                     /locus_tag="VFK23_04490"
     CDS             3054..3770
                     /locus_tag="VFK23_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET7318377.1"
                     /translation="MTTPSDIIRAALHEGRKTLFEHEAKELARSVGIAVPKYILAGAA
                     DPQAVAEAGESLGFPVVLKAVSPDILHKTDAGAVMLNITSRSMLAASVTAMKNMISAR
                     APGAVVNAFLVEKMMPQGVELLIGGMRDEQFGPSVAFGLGGVWVEALKDAVFGILPMS
                     HEETVDMISKTRAGMFLRGFRGGPALDEEAVHLIITNLSNLLFDHPEIREIDMNPVRV
                     YPRGAMALDVRVVLNNPSSS"
     gene            3894..>4446
                     /locus_tag="VFK23_04495"
     CDS             3894..>4446
                     /locus_tag="VFK23_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318378.1"
                     /translation="MNRTKSFNELTFLRAEVKRLRKALMRQSPGIDVLLKRRGFKVYK
                     KEPPEDLLVPSRKYLAGYYRMLHKYSFRLLLRDVIKHQEFFTLEDVTRYATPEVTREY
                     LDYLLSVRLIKKRPKGYTLAGGPITSFGATLEWFIAEILKREFGAETVWGVKFKRPRV
                     GGDYDVIARFDGSLMYLEVKSSPP"
BASE COUNT          950 a         1339 c         1326 g          831 t
ORIGIN      
        1 tcgaatgtga cctccggcct gccgaaggac ctgaagtcca tcgtggcctc ggcctatctc
       61 tcctacctcg cgggcgcggg actgagcgca gccatggtga acgtgaacga cgccgagatc
      121 atgaaggtgg tgtacctcat caagacgttc cgtgacgaga tcaccttcgc cgccggggac
      181 ctggcgtaga tcagaaattg ggaaggtagg aagttgagaa ggtgcgtgta cggacttcac
      241 aacttcttac cttctcaccc tctcaccttc gtggtactca ctcacctctt cgaatattcc
      301 ttctgtactt ccggaatgag ttccttgtag tcctcatagg ggaccttgga atgccacttg
      361 cctgcggcca tgccgatgcc gaccacgaga tagaagagag caacaaggac gacgggaaac
      421 agccaggcct tgacgatgcc gacctttttg cccgtactca ccgtgagatt gagcgcgcct
      481 tccgagggac agcgggagac gcaggtaagg cacccgtagc attccgcgga tttgaccacg
      541 gcggtgttct ccacatcgat atgggtgggg cagtgcctgg tgcaggcgtg gcagtggccg
      601 cacttctcct cgttgcgcct caccttgaag gggctcaacc gtgacacgag gcccaggagc
      661 gccccgtagg gacagaggta tcggcaccag acgttcttat acaggaagga aagcgcggcc
      721 agcgcgatca ggacccacat cgtcaccgtc gacatctcgg tgaagaacat catcatccgc
      781 acgtccacgg tcttgtaata gtcgccgata aagaacatcg ccatcgacca catgggcatg
      841 accccgatgg caagcagaaa aaagcccatc agcacgtatt tgatcgaccg aagcgaggtg
      901 tccgaaaaac ccgtgatgcg gaagttcctg ccgaagatct tttcccccac catccagaag
      961 tactgcgaca gcgtcccgac ggggcagatc cagccgcaga aacctttttt catgaccagc
     1021 gacacgccca gaatggccgc gaacaggacg aaccccgcgg gatggacagg atctacggtg
     1081 ccggtaagga taaaatactt gaaggccatg agcccgccga tcggaagaaa tgcgtcgact
     1141 gacgcgggcc gttcgacaaa ggggctgccc gcctcgaagt gcaggacgaa ctcgtagaag
     1201 cgccagccga tgtaaagcgt gaggaggagg aatccgaatt gcaccgcgta gcggagcgga
     1261 cggaggtatg ttctcggtaa tttcatgggt ggcataatac cgtgttcggg cagggaaatc
     1321 tatgacaaag gtcataatgc ggcctggtcg cgataaacac cctctccttc aaggagaggg
     1381 ccgagatgag gatgggtttc ttgccgatcc gacgagaccc atccccaccg taatcctccc
     1441 cttgaagggg aggaaacgtt ctcacacctg tgcgaacagg taaattacct gaaatgtgta
     1501 gcacatttgt tgcaagcgtc tcatgagtat gctatttatt tagcatgtcc aacgacctcg
     1561 acgggttctt ccatccgaaa acggttgccc tcatcggcgc cacggacaat cccctgaaac
     1621 ccggcgcggc gatcctggag aacctgtcgc attaccgcgg cgccgtcttc ccggtgaacc
     1681 cgaagcacca aacgctcctg ggacgcaggt gttacccctc ggtggcgtcg gtccccgatg
     1741 agatcgatct cgccattgtc gcgttgccgg ccggcctcgt ggagcaggag ctcgcgcgcc
     1801 ttcacgataa gggaataagc cgcgcgatcg tgattagctc cggctttgcc gaggcgggag
     1861 ggcccggcat cgaccttcaa aagcggctct cggaaacggc gaagagcttc ggcatccgga
     1921 tcatcggacc gaacgcactc ggcattttca acacggacaa cgggttcgac agctttttcg
     1981 tgtcccgtca ccgcgtttcc cgtcccgctg cgggaaggct ttcgatcatc tcccagagcg
     2041 gcgccatcac ggtgatcctg atggaggcct tcgcccgcga cgatatcggg atcgccaagg
     2101 ccgtaaacta cggaaaccgg ctcgacatcg atgacgcaga ctgcctcgac tatttcgccg
     2161 atgaccccca gaccggagct gtggccatgt acatggaatc cgtggcggag ggaagaaagt
     2221 tcctaaagtc cgcgaaggcg ttcgtggcga agaaaccgct gatcgtctgg aaggcgggca
     2281 agcacgagct gggcgcctcc gcagtagcat cgcacacggc gacgcttgcc ggcaggtacg
     2341 gcctctacca ggccgccttc cgtcaggccg gggccgtcga ggtacagggt ttcgaccaca
     2401 tgatcgacgc ggcgaaggcc gtcgcgctca tggagcatcc ctgcttcgga aacaggctcc
     2461 tcattgtcac gaacggcgga ggcatggccg tcgcggcgtc ggaccaggct cagcaggagg
     2521 ggttctcaat gcctcctgtc ccggaacagg tcaggaaaag actcgatgcg gccctgcctc
     2581 tcttcttcgg caagaacaat cccatcgacc tcaccggaag cgggagcaac gaagattact
     2641 acaccgcgct tacggaggcc ctcccgcatt atgacgcggc cgttatcatc gtgctcatgg
     2701 gatcgacgac ggtgaccgac caggcgacgg agctgatcgc acgcgcgtgc cgcgacgcga
     2761 aaaagccggt cacctgctgc atcctccagg ggctgggtta cacgcgggag gcgatgaaca
     2821 cgctcctcag gcacggcatc cccgtgtacc cgtcgccgga gagggctgtg agggcgctgg
     2881 ccgtgctgag gaaggcgaca cacccagaga tcaaaatcga gggggcgaaa cggtgaacgg
     2941 gggagtaggc gacacagcaa atcttggctg cttttttttc gccccctcgc cgattcgccc
     3001 ctcgcccact cgttgcctcg gatggcagaa gctcattgtt cgataatagc attatgacga
     3061 caccctcaga catcattcgg gcggcactcc acgagggccg caagacgctc ttcgagcacg
     3121 aggccaagga gctcgcgcgt tcggtaggca tcgccgttcc gaaatatatt ctggccggcg
     3181 ccgccgatcc ccaggctgtc gctgaggcag gcgagtcgct cggatttccc gtggtgctca
     3241 aggccgtgtc ccccgatatc ctgcacaaga cggacgcggg cgcggtgatg ctgaacatca
     3301 cttcccggtc gatgcttgcg gcctcggtta cggcgatgaa gaacatgatc tcggcgcggg
     3361 cgccgggggc ggtcgtgaac gcgttcctcg tcgagaagat gatgccgcag ggcgtcgaac
     3421 tcctgatcgg cggcatgcgg gacgagcagt tcggcccgtc cgtggccttc ggtctcggcg
     3481 gcgtctgggt cgaggcgctc aaagacgccg tcttcgggat cctgcccatg agccatgaag
     3541 agactgtcga tatgatttca aagacccggg caggcatgtt cttgaggggc ttccgcggcg
     3601 gacccgcgct cgacgaagag gccgtgcacc tgatcatcac caacctttcg aatctcctgt
     3661 tcgatcatcc cgagatcagg gagatcgata tgaaccccgt gcgcgtctac ccccgcgggg
     3721 ccatggcgct ggatgtcagg gtcgtactga acaacccatc ctcctcctaa cctcccccct
     3781 tgaagggggg aggcactcgc atgttccctc cccttcattg ccgataaaac gggccacgct
     3841 gaggggaggg caagggtggg gatgggtctc gaccgtggac gacttaaatt accatgaacc
     3901 gcaccaaatc cttcaacgaa ctcaccttcc tccgggccga ggtcaagcgg ctccgcaagg
     3961 cactcatgcg gcagtcgccg ggcatcgatg tccttttgaa gcgccgcggt ttcaaggtct
     4021 acaagaaaga acccccggaa gaccttctgg tcccttccag gaaatacctg gccggttatt
     4081 accggatgct ccataaatat tccttccggc tcctgctgcg cgatgttatc aagcaccagg
     4141 agttcttcac cctcgaagac gtcacgcgct acgccacgcc ggaggtgacg cgggagtacc
     4201 tcgactatct cctgtcagtg agactgataa aaaaacggcc gaagggttat accctcgctg
     4261 gagggcccat taccagcttc ggcgccacgc tggaatggtt catcgccgaa atcctcaagc
     4321 gggaatttgg tgccgagacg gtctggggcg tgaagttcaa gaggccgagg gtgggcgggg
     4381 actacgacgt gatcgccaga ttcgacggct cgttaatgta cctggaagtg aaatcttctc
     4441 ccccga
//
LOCUS       DASSLD010000247         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_230247,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000247 DASSLD010000000
VERSION     DASSLD010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230247"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /gene="nrfD"
                     /locus_tag="VFK23_04500"
     CDS             complement(<1..505)
                     /gene="nrfD"
                     /locus_tag="VFK23_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET7318379.1"
                     /translation="MGNLIKNFFIALRQIFIGDRTYYLWVAFLSFFILLGLSAYFDQL
                     NRGLITTAMRDQVSWGLFISNFTFLVGVAAAAVLLVIPAYLYNFKPIKEIVLFGELLA
                     ITAITMCILFILVDMGQPARVWHILPIIGAMHFPASLLAWDVVVLNGYLAINSIIAFY
                     VLYRLSIG"
     gene            complement(517..1443)
                     /locus_tag="VFK23_04505"
     CDS             complement(517..1443)
                     /locus_tag="VFK23_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7318380.1"
                     /translation="MSKIMKKKDGIHLDRRSFLKGAALGLAGAALPLNKADASFWESF
                     FQKHFKEMNDAEIKQVLSRLEREYEEKYKKAIKVGNEKPMPGVLFGYGLDLSRCIGCR
                     RCEYACVDENNQSKDPQIHWISVLRFKKGEKWVDLEGSEKYYNPELVPEEEYFYMPVQ
                     CQQCENPPCVRACPTQATWKEPDGIVVVDYNWCIGCRYCMAACPYGARHFNWTEPGRK
                     PEEINPQTHYLGNRPRYKGVVEKCTFCIQRTRNGRYPACVEACPVGARKFGNLLDPNS
                     EIRYAIEHKRVSRLKEDLNTNPKFYYFFAYGR"
     gene            complement(1476..1967)
                     /locus_tag="VFK23_04510"
     CDS             complement(1476..1967)
                     /locus_tag="VFK23_04510"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET7318381.1"
                     /translation="MLWLIVFLAVIGIPVVAASEEQDIPKKFFVPPPPFSPGIFPCSQ
                     CHQGMPVNRRPRKLEQMHQDITLKHMPGGWCFDCHNPDNRDKLRLANGKLISFEESYN
                     LCGQCHGTILREWKAGLHGKRTGMWNGEKQYRLCVHCHWPHDPQFKPIKPLPPPVRPT
                     DIK"
BASE COUNT          414 a          575 c          609 g          481 t
ORIGIN      
        1 tcccgatcga cagccggtag aggacataga aggcgatgat gctgttgatg gcgagatagc
       61 cgttcaatac gaccacgtcc catgcaagca gcgatgcggg gaaatgcata gcgccgatga
      121 tcggcaggat gtgccagacg cgcgcgggct gtcccatgtc gaccaggatg aacaggatgc
      181 acatcgtgat ggcggttatg gccaggagtt ccccgaagag cacgatttcc ttgatcggtt
      241 tgaagttgta gaggtacgcc ggaatgacca gcagcacggc cgcagctgca acgccgacaa
      301 ggaacgtgaa gttcgagatg aacaggcccc aggagacctg gtcccgcatg gccgtcgtga
      361 tgaggccgcg gtttagctgg tcaaaatagg cgctcaggcc gaggagtatg aagaatgaaa
      421 gaaacgccac ccagagataa taggtgcggt ccccgatgaa tatctgacgc agggcgataa
      481 aaaagttttt gatcaaattg cccatgagcg agcctcctac ctgccgtatg cgaagaaata
      541 gtagaatttg gggttggtgt tcaggtcttc cttcaagcgg gacacccgct tgtgctctat
      601 ggcgtaccgg atctcgctgt tggggtcgag gaggttcccg aacttccgcg cgccgaccgg
      661 gcaggcctcg acgcacgccg gataccgccc gttccgcgtt ctctggatgc agaacgtgca
      721 cttctccacc acccccttgt agcgcgggcg gtttccgaga tagtgcgtct gcggattgat
      781 ctcctcgggc ttccggcccg gttccgtcca gttgaaatga cgcgcgccgt agggacaggc
      841 ggccatgcaa tagcgacagc cgatgcacca gttgtaatcg acaaccacga ttccgtccgg
      901 ttctttccag gtggcctgcg tgggacaggc tctcacgcag ggcgggttct cgcactgctg
      961 gcactggacc ggcatgtaga agtactcctc ctcgggcacg agttcgggat tatagtattt
     1021 ctccgaaccc tcgaggtcga cccatttctc gcccttcttg aaccggagga cggagatcca
     1081 gtggatctgc gggtctttgg actggttgtt ctcatcgacg caggcatact cgcatctcct
     1141 gcagccgatg caccgggaaa gatcgaggcc gtagccgaac aggacccccg gcatgggctt
     1201 ttcatttccc accttgatcg ctttcttgta tttttcctca tattcccgtt ccaggcggct
     1261 caggacctgt tttatctcgg cgtcgttcat ttccttgaag tgcttttgaa agaacgattc
     1321 ccagaatgat gcatcggcct tgttcaacgg gagcgcggct cccgccagac cgagtgccgc
     1381 tcctttgaga aagctcctcc ggtcgagatg gattccgtct tttttcttca tgatttttga
     1441 catcggtctc ctcattatcc tcgccgtgtt tttctttact tgatgtcagt cggcctcacc
     1501 ggcgggggaa gcggtttgat cggcttgaac tgcggatcgt ggggccagtg gcaatgcacg
     1561 cacaggcggt actgcttctc gccgttccac atgccggtcc tcttcccgtg caggcccgcc
     1621 ttccactcgc gaaggatcgt gccgtggcac tggccgcaga gattgtagga ctcctcgaaa
     1681 ctgatcagct tgccgttcgc aagccgaagt ttgtcccggt tgtccggatt atggcagtcg
     1741 aagcaccagc cgcccggcat gtgtttcagg gtgatatcct ggtgcatctg ctccagcttt
     1801 cgcgggcgtc tgttgaccgg catgccttga tggcactggg agcaggggaa aatgcctggt
     1861 gaaaagggag gaggaggcac gaaaaacttc ttggggatat cctgctcttc tgaagcggcg
     1921 accaccggga tcccgatgac cgcgaggaac acaatgagcc aaagcatacg tcctgatttc
     1981 attttgatgt gcatgtcgac ttcccctttt taagtggggt ggctgctcgg cgaacggagg
     2041 caatcctctc taatcgttct tccgtgatat caggaatat
//
LOCUS       DASSLD010000248         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1099686, whole genome shotgun sequence.
ACCESSION   DASSLD010000248 DASSLD010000000
VERSION     DASSLD010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099686"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /gene="mraY"
                     /locus_tag="VFK23_04515"
     CDS             <1..789
                     /gene="mraY"
                     /locus_tag="VFK23_04515"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET7318382.1"
                     /translation="WVVLFATLAFGGIGFWDDYLKVVKKRSTGLRAFQKFGLQIMASL
                     AIAFFLYFDKSDPNSTILSLPFMKQLFLDLGWFYVPFVVIVIVGSSNAVNLTDGLDGL
                     AIGLVGIASTANAVIVYLGGNRVIANYLNILYIPGSGELAIFCGAMLGASLGFLWYNA
                     HPAEVFMGDVGSLSLGGALGTLAVVTKHELILIIVGGIFVAETVSVMLQVASYKLTGK
                     RIFKMAPIHHHFEQIGWPESKVIVRFWVVGIILALVSIGSLKLR"
     gene            941..>2102
                     /gene="murD"
                     /locus_tag="VFK23_04520"
     CDS             941..>2102
                     /gene="murD"
                     /locus_tag="VFK23_04520"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET7318383.1"
                     /translation="MTVVGLARSGVAAARALDALGAIVTVTDRKPLAELGAQLAALGS
                     GRIAVEAGGHPERIFLGADLIVLSPGVPKIPPVLEARRRGVKVISELELAWLLADSPF
                     VGITGTNGKSTVTTLVGLMLERAKKKVLVAGNIGNALTEDVSLLRGRDWIVAELSSFQ
                     LEDIDTFRPKVAAVLNVTQDHLDRYHDIEEYAEAKARIFMKQGREDFLVLNFDDPLVK
                     SYARRARARVVPFSRLLRFNPGACVRDGFLEYNGRRIMRVEEIRIKGVHNLENALAAA
                     ALARCAGADDESVAAVLREFPGLEHRLEFVREKNGVTYINDSKGTNVGAVVKSVEGFT
                     QPVILIAGGLDKGSDFSPLAELFKRKVKLLILIGKAAEKMGKALGTATETVYA"
BASE COUNT          375 a          642 c          677 g          408 t
ORIGIN      
        1 tgggtcgtgc tcttcgccac ccttgccttc ggcggcatcg ggttctggga cgactacctc
       61 aaggtcgtca agaaacggtc caccggactc cgggcctttc agaagttcgg gctccagatc
      121 atggcgtcgc tcgccatagc ctttttcctc tactttgaca agagcgaccc caacagcacg
      181 atcctgtctt tgccgttcat gaagcagctg tttctcgatc tgggctggtt ttacgtgcct
      241 ttcgtcgtca tcgtgatcgt cggctcctcc aacgccgtca acctgaccga cggcctggac
      301 ggcctggcga tcgggctcgt cgggatcgcg tccaccgcca acgcggtgat tgtgtatctg
      361 ggcgggaaca gggtcatcgc caactatctc aatatcctct atatccccgg cagcggcgag
      421 cttgccatct tctgcggagc catgctgggc gcgagcctcg gattcctctg gtacaacgcg
      481 cacccggccg aggtgttcat gggcgatgtg ggctccctct ccctgggcgg cgccctcggc
      541 accctggcag tggtgaccaa gcacgagttg atcctgatca tcgtcggcgg cattttcgtg
      601 gccgagaccg tctcggttat gctccaggtg gcgtcgtaca aactgacggg aaaacggatc
      661 ttcaagatgg ccccgatcca tcatcatttc gagcagatcg gctggcccga gtccaaagtc
      721 atcgtccggt tctgggtcgt ggggatcatc ctggcgctcg tctcgatcgg gtcgctgaaa
      781 ctgaggtagt ggcggaaatg tgagaaggta ggaagataag aaggtaagaa ggtaggaaag
      841 aagcggcgat gcaggttttt tcccaccctc ctaccctccc accttcccac gttcggattt
      901 aacaggaggt atggtgcaac cggattttag ggggaaaaaa gtgacggtgg tcggccttgc
      961 gcggagcggc gtcgccgctg cgcgggccct cgacgcgctc ggcgcgatcg tgacggtcac
     1021 ggacaggaag cccctcgcag agctcggcgc gcagctcgcg gccctcggca gcggccgcat
     1081 cgccgtcgag gcgggcggcc atccggagcg catcttcctc ggggccgacc tcatcgtgct
     1141 ttctcccggc gtcccgaaga tccctccagt cctggaggca cggcgccgcg gcgtgaaggt
     1201 gatcagcgag ctggagcttg cctggctgct cgccgattcc cccttcgtgg gcatcacggg
     1261 cacgaacggc aagtccacgg tcacgacgct agtggggctc atgctggaac gggcgaagaa
     1321 aaaggtgctt gtggcgggga acatcggaaa cgccctcacg gaggacgtct ccctgctccg
     1381 gggccgggac tggatcgtcg ccgaactgtc gagctttcag ctcgaggaca tcgatacctt
     1441 ccggcccaaa gtcgccgctg tcctgaacgt gacgcaggac caccttgacc ggtaccatga
     1501 catcgaagag tacgcagagg caaaggcgcg gatcttcatg aagcagggga gggaggattt
     1561 tctcgtcctc aatttcgacg atcccctcgt gaagtcctac gcccggcgcg cccgggcaag
     1621 ggtggttccc ttttcccgtc tgctgcggtt taatccgggt gcctgcgttc gggacggctt
     1681 tctggagtac aacggcaggc gcatcatgcg ggtggaagag atcaggatca agggcgtgca
     1741 caatctcgag aacgcccttg ccgccgcggc gcttgcccgg tgcgcgggtg cggacgacga
     1801 gtccgtcgcc gccgtgctcc gcgagtttcc cggcctcgag caccggctcg agttcgtgcg
     1861 cgaaaagaac ggcgtcacct acatcaacga ttccaagggt acgaacgtgg gggccgtggt
     1921 gaagtccgtc gagggtttca cgcagccggt gatcctgatc gccggcggcc tcgacaaggg
     1981 gagcgacttc agccccctcg ccgagctctt caagcggaag gtgaaactcc tgatcctgat
     2041 cgggaaggcc gcggagaaga tgggcaaggc gctcggaacg gcgacggaga ccgtgtacgc
     2101 gg
//
LOCUS       DASSLD010000249         2360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_434171,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000249 DASSLD010000000
VERSION     DASSLD010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434171"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /gene="rtcA"
                     /locus_tag="VFK23_04525"
     CDS             complement(<1..974)
                     /gene="rtcA"
                     /locus_tag="VFK23_04525"
                     /inference="COORDINATES: protein motif:HMM:NF003246.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HET7318384.1"
                     /translation="MPPSNHNIIKIDGSIGEGGGQILRTSLALSCVLRLPVEIVNIRR
                     ARQKPGLQPQHLTAVNAAAAVCRAKVEGARLSSTAIRFLPDAVVGGDYYFDVSEKKGS
                     AGSAPLVLQTILPPLCFADGPSTVTVVGGTHVPWSPTAHYLEQVFLPMLARIGADASV
                     FLDTWGWYPVGGGKLTARIRPVKEPLPLRVTERGMLARVGGISAVSNLPREIAVRQRD
                     RAIRTLSQKGIDAEIELVNAPSPGKGTLVFLLSEFENVRVGFSALGASGKRAEEVAEE
                     ACRYLFEYLDAGALLDPHLADQLVHYLALARGASEFTTSRITKHLLTN"
     gene            1233..1694
                     /locus_tag="VFK23_04530"
     CDS             1233..1694
                     /locus_tag="VFK23_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET7318385.1"
                     /translation="MSNTSVILRCRACRTLNRVPGEKLASRPHCGQCKAQLDFPRAPL
                     NVTAASYDREVNDWPEILVAEFWAKWCGYCRMMEPSINDLAARRAGRLKVIRVDVDSE
                     QPLARRFTIKATPTFILYRNGRQLARMDGAPQQQGELENWIDRMTAQTASP"
     gene            1845..>2360
                     /locus_tag="VFK23_04535"
     CDS             1845..>2360
                     /locus_tag="VFK23_04535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7318386.1"
                     /translation="MHRSRTIPTAVALALFLLWITPGLLGRDLWKADEPYSFGLVHHI
                     VETGDWVIPTIAGQPFMEKPPLFYLTAAGFVRLFSSWIEPHDAARLASALFMLLTTVF
                     MGLAARELMGEKAAGMTIVLLVGSTGLQVTSHKLITDLALLTGIAAALYGFTLFLRRP
                     ALGGFWIGTGTG"
BASE COUNT          470 a          720 c          680 g          490 t
ORIGIN      
        1 atgttcgtga gcaggtgctt cgtaatgcgg ctcgtcgtga attccgacgc gccccgcgca
       61 agggcgaggt agtggaccaa ctggtccgcc aggtgcggat cgagcagggc gccggcgtcc
      121 aggtattcga acaggtatct gcacgcctcc tcggccactt cctccgccct tttgccagag
      181 gcgccgaggg cgctgaagcc cacacgcacg ttctcgaact cggagagcag aaagacgagc
      241 gtgcccttgc ccggcgaagg cgcgttcaca agctcgattt ccgcgtcaat gcccttttgc
      301 gaaagcgttc tgattgcccg gtcccgctgc cggaccgcga tctcgcgcgg caggtttgaa
      361 acagcggaga tgccgcccac gcgcgcgagc atcccccgtt cggtcacacg gagcggaagc
      421 ggttccttca ccggcctgat gcgtgcggtc agtttccctc ctcccaccgg ataccaaccc
      481 cacgtatcaa ggaagacgct ggcgtccgct ccaatacgcg cgagcatagg aagaaacacc
      541 tgttccagat aatgcgcggt aggactccag ggcacgtgcg tgccgccgac caccgtgacc
      601 gtagacggtc catccgcaaa acaaagcggc ggcaggatcg tctggaggac gagtggcgcg
      661 ctcccggcgc tccctttttt ttcggaaacg tcgaaatagt aatcaccgcc gacgacagca
      721 tccggcagga agcggatggc cgtggacgaa aggcgcgccc cttcgacctt cgcccggcag
      781 acggcagctg ctgcattaac agcggtgagg tgctgcggct ggagacccgg cttttggcgg
      841 gctctgcgga tattgacgat ctctacgggg aggcggagga cgcaggagag tgccagagag
      901 gtcctgagaa tctgtccccc gccctctccg atggaaccat cgatcttgat tatgttgtga
      961 ttcgatgggg gcatgtcgat acctgattat acacgaatct ttcaggcagt ttcgtcccgc
     1021 atgcggcctg ttcgctgcat actgtgaaaa gcaaatttgg ccgcgaaaag gcgcaagatg
     1081 cacaaaatta ggaaccggaa atggatatat gttttttgcg gctcttacgc atctctgcgg
     1141 ccaaatatct tttgtgttgt ggttgcggtc aaaggccttg ctatgatata ttcttagcat
     1201 ttaagaaaac ttcgtattcc gaaggacatc ccatgtcgaa cacttccgtc atcctgcggt
     1261 gcagggcctg cagaacgctg aaccgcgttc ccggggagaa actggcgagc cgtccccact
     1321 gcggacagtg caaggcgcag ctcgactttc cccgtgcacc cctcaatgtg accgcggcga
     1381 gctatgaccg tgaggtaaat gactggcccg agatcctggt tgcagaattc tgggcgaaat
     1441 ggtgcggata ctgccgcatg atggagccct ccatcaacga tctcgctgcc cggcgcgcgg
     1501 gcaggctcaa ggtgatccgg gtggatgtgg actccgaaca gcccctcgcc cggcgtttca
     1561 cgatcaaggc aaccccgacc ttcatcctct accggaacgg ccggcagctc gcgcgcatgg
     1621 atggcgcacc ccagcaacag ggcgagctgg aaaactggat agaccgtatg acggcacaaa
     1681 cggcatcccc gtagggacgt tccctttgag cgcggttatc cctcctgcgt atcctggttc
     1741 gtcatgtttt cgaaaaatgt tcgtacgttt tgaacatcat gcctcagtct ggtatacaat
     1801 atatagaata taggcttgct tcgctaaaag tccactgaca aaatatgcat agatcgcgaa
     1861 ccataccaac cgccgttgcc ctcgccttat tccttctctg gattacgccg gggctcctgg
     1921 gccgggacct ttggaaggct gatgaacctt acagcttcgg cctcgtgcac catatcgtcg
     1981 agaccggcga ctgggtcatc ccgacgattg ccggccagcc tttcatggaa aagccccctc
     2041 tcttttatct caccgcggca ggcttcgtcc gccttttctc ctcctggatt gagcctcatg
     2101 atgccgcgag gctcgcttcc gccctgttca tgctcctgac cacggtgttc atggggcttg
     2161 cagcgagaga actgatgggg gagaaagccg ccggaatgac gattgtgctc ctcgtcggaa
     2221 gcacgggcct ccaggtcacg tcgcacaaac tgatcaccga tctcgcgctc ctcacgggca
     2281 ttgccgccgc cttgtacggc ttcaccctct tcctgaggcg gcccgcactg ggtggcttct
     2341 ggatcgggac cggcaccggc
//
LOCUS       DASSLD010000250         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_560163,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000250 DASSLD010000000
VERSION     DASSLD010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560163"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFK23_04540"
     CDS             <1..330
                     /locus_tag="VFK23_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET7318387.1"
                     /translation="AKALEQVRSALAGVVAEPGGTGHAARTTIATVAGKTGTAQVIGQ
                     KVAGRKLSAKTGDHAWFVAYAPVEHPKIAVAVVVEHGGHGGSAAAPIAKRVIEEYMKN
                     VNQTLVR"
     gene            305..1435
                     /gene="rodA"
                     /locus_tag="VFK23_04545"
     CDS             305..1435
                     /gene="rodA"
                     /locus_tag="VFK23_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HET7318388.1"
                     /translation="MLTKRWFAKLPWGMIALVFGIACIGLTAVYSATYTLRGPSPLFY
                     KQLIWISLGIVIMFVSIIPDYHTVGRYAYVLYAISLVLLVLVMAFGRTGMGAQRWLAI
                     GPFVFQPSEFAKLSVTLALARFFAEDPKRGDYGLKDLSVPGAMVLVPLVLVLKQPDLG
                     TAIMLLLTASLIAMLAGIHLRALAVVLLSAAVVITLVFFVSPVRNKIWGSLKPYQQNR
                     IWAFVDPGSDPLGSGYHANQSKIAVGSGQITGKGYRKGTQSQMAFLPERHTDFIFAVI
                     AEETGFVGSSTIVLLYLALLLVGVDTAKNAKDRLGVLMAGGIVSMISLYVFINVGMAV
                     GIVPVVGVPLPLVSYGGTSILATFLALGLLLNIQTRRFELFY"
     gene            1520..>2117
                     /locus_tag="VFK23_04550"
     CDS             1520..>2117
                     /locus_tag="VFK23_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HET7318389.1"
                     /translation="MLEDFLPLVSKPGRYIDSEINAVHKDLSRVATKVCLFFPDAYEV
                     GMSHLGLRILYHILNKRPDTACERVFSPWPDYEEKLRASGRPLRSLESGLPLAEFDII
                     GVTLQYELSYSNILAGLELAGIPLRSSQRNDGHPVIVAGGPCAVNPEPLSDFVDVFFI
                     GEAEEAIHELIETRQAERSRSAFLEVISRHEGFYVPALG"
BASE COUNT          374 a          687 c          618 g          438 t
ORIGIN      
        1 gccaaggcgc tcgaacaggt gcggtccgcc ctggccggcg ttgttgcaga accgggcggc
       61 accggccatg cggctcgtac gacgatcgcg acggtggcgg gaaaaacagg caccgcccag
      121 gtcatcgggc aaaaagtggc cggccggaag ctttcggcaa aaaccggcga ccacgcgtgg
      181 ttcgtcgcct atgcgccggt ggaacatccg aagatcgccg tggcggtggt cgtcgaacac
      241 ggcgggcacg gcggctcggc ggcggcgcct atcgcaaaaa gagtcatcga ggagtacatg
      301 aaaaatgtta accaaacgct ggttcgctaa gctgccctgg ggcatgatcg ccctcgtgtt
      361 cggcatcgcc tgcatcgggc ttacggcggt atacagcgcg acatacacgc tccgcggtcc
      421 gtcgccgctc ttttataaac aactcatctg gatatccctc ggcatcgtca tcatgtttgt
      481 ttcaatcatt cccgattatc acacggtggg ccgctatgcc tacgttctgt acgccatctc
      541 gctcgtgctc ctcgtcctcg tcatggcgtt cggacggacc ggcatgggag cgcagcggtg
      601 gcttgccatc ggccccttcg tttttcaacc gtcggaattc gcgaaactct cggtcacgct
      661 cgccctggcg cgcttctttg ccgaggaccc gaagcggggc gactacggcc tcaaagacct
      721 gagcgtgccg ggcgcgatgg ttctcgtccc cctcgttctg gtgctcaagc agccggacct
      781 cggcacggcg atcatgctgc ttctgacggc ctcgctcatc gccatgctgg cgggcatcca
      841 cctgcgggcc cttgcggtcg ttctgctctc ggccgcggtc gttataacgc tggttttttt
      901 cgtttcgccg gtgaggaata aaatctgggg ttctctcaaa ccctatcagc agaacaggat
      961 ctgggccttt gtcgatccgg gctccgatcc tctgggcagc ggctaccacg cgaaccagtc
     1021 gaagatcgcg gtgggctcgg gccagatcac gggcaaggga taccgcaagg ggacgcagag
     1081 ccagatggcg tttcttccgg agcgccatac ggacttcatc ttcgccgtca tcgccgagga
     1141 gaccgggttt gtcggctcct cgaccatcgt cttgctctac ctggcccttc tcctcgtcgg
     1201 cgtcgatacc gcaaaaaacg caaaagaccg actcggcgtt cttatggccg ggggcatcgt
     1261 ctccatgatc tcgctctacg tattcatcaa cgtgggcatg gccgtgggca tcgtgcccgt
     1321 tgtcggcgtg cccctcccgc tcgtgagtta cggcggcacg tccatcctcg cgacctttct
     1381 cgctctcgga ttgctcctca atatccagac gcggcggttc gagctgttct attaacatca
     1441 ggggccaggg tcccgtagca cgggtccagg aatcaaaaac ctggaccccg gaccccggac
     1501 cccggaccct ggggttttca tgctcgaaga ctttctcccg cttgtatcga aaccgggcag
     1561 gtacatcgac agcgagatca acgcggtcca caaggatctc tcgcgggtcg cgacgaaggt
     1621 ctgcctcttc tttccggacg cctatgaggt gggcatgtcg cacctgggcc tgcgcatcct
     1681 ctaccacatc ctgaacaaga ggccggacac ggcctgcgag agggtcttct ctccctggcc
     1741 ggattacgag gaaaaactgc gggcgtcggg gcgcccgctc agaagcctcg aatcgggcct
     1801 ccccctggcc gagttcgaca tcatcggcgt cacgctccag tacgagctct cctattccaa
     1861 catccttgcc ggcctggagc tcgccggtat tcccttacgt tcatcgcaga gaaacgacgg
     1921 acatcccgtc atcgttgcgg gcggcccctg cgccgtgaac ccggaaccgc tctccgattt
     1981 cgtcgacgtt ttcttcatcg gcgaagcgga agaagcgatc catgaactca tcgaaacaag
     2041 acaggccgag cggtcccggt ctgcctttct cgaggtaatc tcgcggcacg aaggctttta
     2101 cgtccccgct ctcggca
//
LOCUS       DASSLD010000251         3436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_586783,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000251 DASSLD010000000
VERSION     DASSLD010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586783"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VFK23_04555"
     CDS             complement(<1..625)
                     /locus_tag="VFK23_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HET7318390.1"
                     /translation="MPDELLLIDKVYKIFPVKAGLGRTLAATAVDGVTLTVVRGEAVG
                     LVGESGCGKSTLGRLALRLLEPTSGAVRFEGKDLGALDKQELRELRKNMQIIFQDPFA
                     SLNPRMRIIDIINEPLVIHGLARKNELQKKGAALLEKVGLTADALRRYPHEFSGGQRQ
                     RIGIARALASGPSFIVADEPVSALDVSVQAQIINLLEDLRDEMKLSFL"
     gene            complement(692..1651)
                     /locus_tag="VFK23_04560"
     CDS             complement(692..1651)
                     /locus_tag="VFK23_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7318391.1"
                     /translation="MDLLEIQGLSTHFFTRAGVIRAVDDLSLRLSKGRVLGLVGESGC
                     GKTVTALSILNLVPYPGKIVSGKILFEGSDLRLISADQMRAIRGAQISMIFQEPMTAL
                     NPVFTVGSQIAEVLTTHRHASKKEALDSSVELLRSVGIPSPEKRAHEYPHQLSGGMRQ
                     RVMIAMAIACRPSLILADEPTTALDVTIQAHILELLSTIQAEMGMAMVLVTHDLGLIA
                     ERAHEVAVMYAGRIVEQADTGELFANPQHPYTRGLMASIPKPGEERRKRLSTIAGSVP
                     RLHDLPKGCSFVPRCDIRTGKCETGPQLVEVKPGHLVRCWEAK"
     gene            complement(1666..2391)
                     /locus_tag="VFK23_04565"
     CDS             complement(1666..2391)
                     /locus_tag="VFK23_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7318392.1"
                     /translation="MPPKLDDLYKVGIEAFDKGDYEKAERLFHEILIANPMFADIQNK
                     LGIIYNQTNRLALAAEAFEKALAINPGYTEASLNLAITYSDLGKYEKAREVFERAAHF
                     TDATGRVMMSTSGNDPFIKGKLADEHFRLGNMYYDLRLLDEAIEEYQKALRLSPNFAD
                     IITRLGISYRDKGLYDAAIKEFNRAKECNPKYIPARLHLGITYYSQGFYGLAEEEWRE
                     ALAIDPANGAIRTYLNFVKPQNS"
     gene            2741..>3436
                     /gene="lpxC"
                     /locus_tag="VFK23_04570"
     CDS             2741..>3436
                     /gene="lpxC"
                     /locus_tag="VFK23_04570"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HET7318393.1"
                     /translation="MIRYQRTLKKSVSCEGIGLHSGKIVKMTIKPAPAGSGIVFSRTD
                     LDGAEVQAIAANTAATSYATTLRQNRISVMTVEHLLAAFAGLNIDNAYVEIDAEELPI
                     MDGSARPFVRLIADAGRQSQVVPQPMLKVISPVFVREGDKQVAVWPAETAGISYFIDY
                     NHPMLKEQSFQYQQSEEAFLCEVSDARTFGFANDVKSLQANGLAKGASLDNAVALGED
                     SILNEDGLRYKDEF"
BASE COUNT          679 a          980 c          998 g          779 t
ORIGIN      
        1 acaggaagga gagcttcatc tcgtcacgca ggtcttcgag gaggttgatg atctgggcct
       61 gcacggaaac gtcgagggcc gagacgggct cgtccgccac gatgaacgac ggcccggagg
      121 ccagcgccct cgcgatgcct atcctctgcc tttgaccgcc ggagaattcg tgcgggtacc
      181 ggcgaagcgc gtcggccgtc agccccacct tctcgaggag cgcggcgcct tttttctgaa
      241 gctcgttttt tcttgccagc ccgtggataa cgagcggttc gttgatgata tcaatgatgc
      301 gcatgcgcgg gttgagcgac gcgaaggggt cctggaagat gatctgcatg ttcttccgga
      361 gctcccgcag ttcctgtttg tcaagggcgc ccaggtcttt tccctcgaag cgcacggccc
      421 cggaggtggg ctccagcagc ctgagcgcga gcctgccgag cgtagacttg ccgcatccgc
      481 tctcgccgac gaggcccacc gcctcgcccc gtacgacggt gagcgtgacg ccgtcaacgg
      541 ccgtcgccgc gagcgtcctg cccagccccg ccttcacggg gaagatttta taaactttgt
      601 cgataagaag taattcgtcc ggcatagcta ttgtcgattg gcgattgttg actgtcgatt
      661 gaaaatccgc attccgaatt ccgcatccgg ttcacttcgc ttcccagcac cgtaccaggt
      721 gccctggctt tacttccacg agttgcgggc ctgtctcgca tttgccggtt ctgatatcgc
      781 agcgcggcac aaaggagcag cccttcggca ggtcatgaag ccggggcacg ctccccgcaa
      841 tcgtgctgag ccgtttcctt ctctcttctc cgggcttggg tatcgacgcc atcagccccc
      901 gtgtataggg atgctgaggg ttcgcgaaca gttccccggt atccgcctgc tcgacgattc
      961 gtcccgcata cattaccgcc acctcgtggg cccgttcggc gatcaggccg aggtcgtggg
     1021 tgacaagcac catggccatg cccatctcgg cctgtattgt gctcagcagt tccaggatgt
     1081 gcgcctggat cgtgacgtcg agtgcggttg tgggctcgtc tgcaaggatc aatgatggcc
     1141 tgcatgcgat ggccatggcg atcatcaccc gctggcgcat gccgcccgag agctggtgcg
     1201 ggtattcatg agcacgcttt tccggagagg gtatgccgac cgagcgcaac agttcgacgg
     1261 aggagtcgag ggcctctttt ttcgatgcat gccggtgggt ggtcaatacc tctgcgatct
     1321 ggctgcctac ggtgaaaacg ggattgagcg ccgtcatggg ctcctggaag atcatcgata
     1381 tctgcgctcc gcggatggcg cgcatctgat cggcggagat cagacgcagg tccgagcctt
     1441 cgaagaggat ctttcccgat acgattttgc cgggataagg gacgaggttc aggatggaca
     1501 gcgccgtcac cgtcttgccg caccccgact cgccgaccag gccgaggacc cgccccttac
     1561 tgagtctgag gctaagatcg tcgacggccc tgatcacccc ggcacgggtg aaaaaatgcg
     1621 tggagagacc ctgtatttcg agaaggtcca acgaagaggc tccgctcagg aattctgggg
     1681 ctttacgaaa ttcaaatatg tccgtatggc accgttggca ggatcgatag cgagcgcttc
     1741 ccgccactct tcttcggcga gaccgtaaaa accttgtgaa taataggtta tgccgaggtg
     1801 caggcgcgcc gggatatatt tggggttgca ctcctttgcg cggttgaatt ctttgatcgc
     1861 ggcgtcgtac aaccccttgt cacggtagga gatgccgagg cgggtaatga tgtccgcgaa
     1921 gttcggcgag aggcggagtg ccttctggta ctcttcaatc gcctcatcaa ggagcctcag
     1981 atcgtagtac atgtttccga ggcgaaagtg ttcgtcggcg agcttgccct tgatgaaggg
     2041 gtcgtttccc gacgtggaca tcatgactct tcccgtcgca tcggtgaagt gcgcggcccg
     2101 ctcgaagacc tcgcgtgcct tctcgtactt gccgaggtcg ctgtacgtga tggcaagatt
     2161 gagagatgcc tcggtatatc cgggattgat cgccagcgct ttttcaaacg cttctgccgc
     2221 gagcgcgagc ctgttggtct gattgtagat gatgcccagc ttgttctgga tatccgcaaa
     2281 catcgggttc gcgatcagta tctcgtgaaa caggcgctcc gctttttcat aatcgccctt
     2341 atcgaatgct tctatgccca ctttatacag atcgtcgagc tttggaggca tggttgctcc
     2401 atgagaggta aattcgccgc ttttttcagc gcgtggaaaa aatttagcat accttgacct
     2461 gatatgtcaa cggcaatcca agagatatgt ggcgaggtac gcggcaagcc tctatggccg
     2521 cagacgatga aaattcccgc gcccctttat ttccactcct ctcccgttcg agtctgtggc
     2581 tccctggcaa cagcatccga agatatgaga ggcttttttg ccacaaaata aactccctgg
     2641 agattaagct gttgtttcag tgactatatt tcacggcgca ttcatggcac gcctattgct
     2701 ttagtatgca ttgtttttgt atgcgaagaa aggatcgctc atgattagat atcagcgcac
     2761 cctcaagaag agtgtctcct gtgaaggcat cgggctccat tccggtaaaa tcgtgaagat
     2821 gacgataaag ccggctccgg caggttcggg tattgtgttc agccgtaccg atttggacgg
     2881 cgccgaggtt caggcgattg cagccaacac cgcggctacg agctatgcaa ccacgctccg
     2941 gcaaaaccgc atatccgtca tgaccgttga gcaccttctg gcggcattcg caggcctcaa
     3001 tatagacaac gcctatgtcg agatcgatgc cgaagagctt ccgatcatgg acgggagcgc
     3061 ccggcccttt gtccggctca tcgccgatgc cggcagacag tcacaagtag tccctcagcc
     3121 catgctcaaa gtaatcagcc cagtcttcgt tcgcgaaggc gacaagcagg tcgccgtctg
     3181 gccggccgag accgccggca tatcgtactt cattgactac aaccatccga tgctcaaaga
     3241 gcagagcttt caatatcagc agtcggaaga ggcgttcctg tgcgaggtgt cggatgcccg
     3301 gacgtttggg ttcgcgaacg acgtcaagag cctccaggct aatgggcttg ccaagggcgc
     3361 ttccctggat aacgccgtgg ccctcggcga ggactccatc ttaaacgagg acggcctcag
     3421 gtacaaggat gaattc
//
LOCUS       DASSLD010000252         2613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_772852,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000252 DASSLD010000000
VERSION     DASSLD010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772852"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFK23_04575"
     CDS             <1..306
                     /locus_tag="VFK23_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7318394.1"
                     /translation="VVKVDVPADRGLITGMYGKALFSIGKRNAILVPRSSIVEMSGVS
                     GVYVVSSEGNAVFQMVQPGEEQGGMIEAVTGLKAGDRVIVAGQVSGMDGRKVVVAQN"
     gene            402..>2613
                     /locus_tag="VFK23_04580"
     CDS             402..>2613
                     /locus_tag="VFK23_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7318395.1"
                     /translation="MKLNISGKITQYFINSQLTVLLMAATAVLGLFALWFTPREENPQ
                     IVVPAANVMVMMPGSSAKEVEELVSKKLEAKLWEIPGVEDVYSVSMNSMSVVTVKFYV
                     GQDKERSLVKLYDKLMSNMDFAPPGASQPLVKPIDVDDVPVVAITLSNSPGASYDDAR
                     LRLVADHVLDELRKVPGVGNTAVIGGRSRQVRVTLDPLRITGYGLSPLQIAGAINVSN
                     ANLLAGDLDHGSKKLALETGGFFEKAGDVRNAVVGVANNKPVYLGDVADVDDGFEETV
                     KLTRIALVGGHTGHGEAVASGGAAGEQPAVTVALAKRQKLNAVKISNEILAKLDELKK
                     TQIPPGIAVTVTRNDGKTANDAVNELVFHLVVSIIVVIILLLFTLGWREAAIVALAIP
                     LTLFITLAVGMVAGQTINRITLFALILSLGLLVDDAIVVVENIHRHYKLQQHSRLQGA
                     ILAVNEIGMPTILATLTVVLAFIPMAFVTGMMGPYMMPIPFNVPVAMITSLVVAFIVT
                     PWASYRLMKAGHDSTPPPLEETRIYRMYRGVLAPLLANGGKRRMFLGVIAVVFLAAMS
                     FPLFQWVKFRMLPKANKNTFLVSIDMPAGTVIEDTDRAARAVGDYIRRMPEVKDYETF
                     VGTGSVIDFNGLLRGGAFREASHYADVRVNLVDKEDRAVSSEKIVLRMRPDIEKLARE
                     SGANIKLVEDPPGPPVRATVVAEIYGPDYNRQREVAADMRTLFAGTDQVVDIDDS"
BASE COUNT          513 a          804 c          813 g          483 t
ORIGIN      
        1 gtggtgaagg tcgatgtccc ggccgaccgg gggcttatca ccggcatgta cggcaaggcc
       61 ctgttcagca tcggaaaaag gaacgccatc ctggtgcccc gctcgtctat tgtcgagatg
      121 tccggcgtct ccggcgtgta cgttgtctcg tccgaaggaa atgccgtctt ccaaatggtc
      181 cagccaggcg aggagcaggg cggcatgatc gaggcggtga cgggcctcaa ggccggagac
      241 cgggtgatcg tggccgggca ggtgtccggc atggacggcc ggaaggtcgt cgtggcacag
      301 aattgaaatg cggaatgcgg aatggaaacc ctgaaaccct ttcctgactg ctctcttcgt
      361 gcccatccgt gtttatccgt ggttaaaaac aggattatct tatgaagctc aatatcagcg
      421 gcaagataac gcagtacttc atcaactctc agcttaccgt gctcttgatg gcggcaacag
      481 cggtactggg cctcttcgcg ctctggttca ccccgcgcga ggagaacccc caaatcgtcg
      541 tgcccgcggc gaacgtgatg gtaatgatgc ccggctcctc ggccaaagag gtcgaggaac
      601 tggtgtcaaa aaaacttgag gccaagctct gggagatccc gggagtcgag gacgtgtact
      661 cggtctcgat gaactcgatg tccgtggtga cggtcaagtt ctacgtgggc caggacaagg
      721 agcgttctct cgtgaagctc tacgacaagc tcatgtccaa catggatttt gctcctcccg
      781 gcgcttccca gccgctcgtg aagcccatcg acgttgacga cgtgccggtc gtggcgatca
      841 cgctttcgaa cagccccggc gcaagctatg acgacgcccg gctccggctc gttgcggacc
      901 atgtgctcga tgaattgaga aaggtcccgg gcgtggggaa caccgccgtg atcggcggcc
      961 ggagccgtca ggtgcgtgtg acccttgacc ccttgcggat caccggttat gggctctctc
     1021 cccttcagat cgccggcgcc atcaacgtct cgaatgcaaa cctcctcgcc ggcgacctcg
     1081 accacggcag caagaaactc gccctcgaga cgggagggtt ctttgaaaaa gccggcgacg
     1141 tcaggaacgc cgtcgtcggc gtcgcgaaca acaagcccgt ttacctgggc gatgttgccg
     1201 acgtggacga cgggtttgag gagacggtga agctgacgcg catcgccctg gtcggcggcc
     1261 atactggcca cggtgaagcg gtggcctccg gcggcgcagc gggtgaacag ccggccgtga
     1321 ccgtggcgct cgcgaagcgg cagaagctga acgccgttaa aatttcgaac gagatccttg
     1381 cgaagctcga cgagctcaag aagacgcaga tcccgccggg gatcgccgtg accgttacgc
     1441 gaaacgacgg caaaaccgcg aacgacgcag tgaacgagct cgtgttccac cttgtggtct
     1501 cgatcatcgt ggtcatcatc ctgctcctgt tcactctcgg gtggcgcgag gcggccatcg
     1561 tggccctggc catcccgctc acgctcttca tcaccctggc cgtgggcatg gtggcgggcc
     1621 agacgatcaa ccggatcacg ctcttcgccc tgatcctctc cctcggcctc ttggtggacg
     1681 atgcgatcgt cgtggtcgag aacatccacc ggcattacaa gctgcagcag cactcgagac
     1741 tgcagggcgc gatcctggcc gtaaacgaga tcggcatgcc gacgatcctc gcgaccttga
     1801 cggtagtgct cgcgttcatc cccatggcct ttgtcaccgg aatgatgggc ccctacatga
     1861 tgccgatacc gttcaacgtg ccggtggcca tgatcacctc gcttgtggtc gcctttatcg
     1921 tgacgccctg ggccagctac aggcttatga aagcgggcca tgactcgaca ccacccccgc
     1981 tcgaagagac caggatttac cggatgtaca ggggcgtcct tgcgccgctg ctcgcgaacg
     2041 ggggcaaacg caggatgttt ctcggcgtca tcgccgtcgt gttcctcgca gccatgagct
     2101 tcccgctgtt ccagtgggtg aagtttcgca tgctgcccaa ggcgaacaag aacacctttc
     2161 tcgtgtccat cgatatgccc gccggaacgg tgatcgagga cacggaccgg gccgcacgcg
     2221 ccgtgggaga ctacatccgg cgcatgccgg aggtgaagga ctacgagacc ttcgtgggca
     2281 ccggttccgt catcgacttc aacggcctgc tccggggcgg ggccttccgg gaggcgagcc
     2341 actacgccga cgtccgggtt aacctggtag ataaggaaga ccgcgccgtc tcaagcgaga
     2401 agatcgttct caggatgagg cccgacatcg aaaaactggc gcgggagtcc ggcgcgaaca
     2461 tcaaactcgt cgaagacccg cccgggccgc cggtccgcgc cacggtcgtc gcagaaatat
     2521 acggcccgga ctacaaccgg cagcgtgagg tcgccgctga catgaggacg ctcttcgccg
     2581 ggaccgacca ggtcgtagac atcgacgaca gcg
//
LOCUS       DASSLD010000253         3518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_592846,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000253 DASSLD010000000
VERSION     DASSLD010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592846"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..912)
                     /locus_tag="VFK23_04585"
     CDS             complement(106..912)
                     /locus_tag="VFK23_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318396.1"
                     /translation="MADFISKHRSAGAFIGKYNFSRELLLFFDVQSLYFFQRPCAELS
                     LPALITLCLRKPAAISAGYGKLAERLAEVLPRRGGEVRLSEPSGEIVFHGNRAKGVRT
                     ARDVVDAKKVLICSSEKSMSSLFLGIRDEVVPVGMEQNVLYLPDYERPRDFLACSLSK
                     KSDASAAPEGMRALAVTFHGSSDPQRGPMDRVARLVPFLKDFIVFSRDFRSSEASAVL
                     PDLSFKPLQSRAGEPLLFKGSRGNVYLLHEARHAPHQVISAARRFVEKTA"
     gene            complement(1066..1326)
                     /locus_tag="VFK23_04590"
     CDS             complement(1066..1326)
                     /locus_tag="VFK23_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET7318397.1"
                     /translation="MSTYDLAVVGAGLGGLAAAALFSAKGKKVLVSSPHASLAEALGV
                     MAADGFLFPSGPVLLYGFEPGGAFNQLFRDSGIHDWEPAHAA"
     gene            complement(1421..3511)
                     /gene="pnp"
                     /locus_tag="VFK23_04595"
     CDS             complement(1421..3511)
                     /gene="pnp"
                     /locus_tag="VFK23_04595"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET7318398.1"
                     /translation="METEIGGKRLILETGVMAKQASGAVVARYGDTVVLSTAVASKVE
                     RENVDFLPLTVDYQEKAYAAGRIPGGFFKREGRQTEKEILSSRLIDRPIRPLFPEGYY
                     FDTQIIASVLSIGDESSMDLMGIIASSAALSISEIPFRGPIGAVRVGFIDDKFVINPG
                     QKELETTRLNLVVAGTADAIMMVEGGATELSEDQMLEALETAHREIKKIVALQNELIA
                     KVGVPKRQVKVVEIDKDLAVQVAALAMERLLTAIVIPNKMERQKTLDALLDEIKQKLK
                     KEDDPTRGRQIATIFFDLEKDEVRKIILEKNVRADGRRPDEIRPISSSVGLLPRTHGS
                     ALFTRGETQALVVATLGTSEDEQRIDSLEGEYFKTFMLHYNFPPFSVGETKPLRGPGR
                     REVGHGALAERALKTMVPTKLEFPYTIRLVSDILESNGSSSMATVCGGTLALMDAGVP
                     IKAPVAGIAMGLIKEGEKVIILSDILGLEDHLGDMDFKVTGTSKGITALQMDMKIEGI
                     TIEVMRTALKQAKDGRLHILGKMLEALAEPRKTLNPFAPRIITMQINPDKIKDVIGSG
                     GKVIRSIVEQTGAKIDIEDSGIINIASSDEAAANKAKEIIRGIVQEAEVGKLYMGKVR
                     KIMDFGAFVEIFPGTDGLLHISQISDRRIEKVTDELKEGDEVLVKVLEIDRQGKIRLS
                     RKEAMRDKEKAKAK"
BASE COUNT          695 a         1049 c          975 g          799 t
ORIGIN      
        1 gtcattcaga cgcgtgctaa atcgaccttt gaaggtatgc ggccgtatca ccggcatcac
       61 acttatattt tgaagggcta gacccccaga aaaagcaata cgcccctacg ctgtcttttc
      121 cacaaatctc cgcgctgccg aaatcacctg gtgcggtgca tgccgcgcct cgtggagaag
      181 atatacattt cctcttgatc ccttgaagag aagcggctcg cctgcccgcg actgcagggg
      241 cttgaacgac aggtcgggga gaacagcgga tgcctcagaa gagcgaaagt cccgcgaaaa
      301 aacgataaaa tcctttaaaa acgggacgag gcgagcgaca cggtccatgg gaccacgctg
      361 tgggtcagag gacccatgga atgtgacggc aagcgcgcgc attccctcgg gcgcggcgga
      421 ggcatcgctc tttttgctga gtgaacaggc gagaaaatca cggggccgtt cataatccgg
      481 aagatacagg acgttctgct ccatgccgac gggcacgact tcgtcccgaa tgccgaggaa
      541 gagggaagac atgctctttt cggaggagca gatcaggacc tttttcgcat cgacgacgtc
      601 tcgtgctgtc ctgaccccct tcgcccggtt accgtgaaac acaatctccc cggacggctc
      661 gctcaggcgc acctcgccgc ccctgcgggg aagcacttcc gcaagtcgtt cggcgagttt
      721 cccgtatccg gcggagatgg cggccggttt gcgtaagcag agcgtaatca gcgccggaag
      781 cgaaagctcg gcgcaggggc gctgaaaaaa atagagggac tgcacgtcaa aaaaaagtaa
      841 taattccctg ctgaagttgt atttcccgat aaacgccccg gcagacctgt gttttgaaat
      901 gaagtcggcc acgcggctat gagagttccg gaccgcagct ttgctcagat cacgatagaa
      961 ttttacgagg gattgaattt cccggggaaa ctctctcctc agttcctcat gtgtttcttc
     1021 ctggtccaga gagaccgtga ttctcctgtc cggcagcgcg acttgttagg cagcatgcgc
     1081 aggttcccaa tcatggatgc ctgagtcacg aaaaagctga ttgaatgcgc cgcccggctc
     1141 aaaaccatac agaagcacag gccccgatgg aaacaggaac ccgtccgcgg ccatgacgcc
     1201 cagggcttcg gccagggacg cgtgcggcga actcaccagt actttcttgc ccttggcgct
     1261 gaaaagcgcg gcagcggcga gccctcccag gcccgctccc acaacggcca gatcgtatgt
     1321 tgacacgctt cctccggttc cgtgaaaata aaaaaaggaa ataggctctg cgagaaaccc
     1381 tatttcctct cctgaacgtc cctatccgca cccgcagtga ttacttggct ttcgcctttt
     1441 ccttgtcccg cattgcttct tttcgtgaga gacggatctt gccctgtcgg tcgatctcca
     1501 ggaccttgac gaggacctcg tctccctcct tcagttcgtc agtcaccttt tcgatccttc
     1561 ggtcggaaat ctgcgatatg tgaagcagcc cgtcggtgcc cgggaatatc tcgacaaagg
     1621 cgccgaagtc catgatcttt cttaccttgc ccatgtagag cttgccgacc tccgcctcct
     1681 gcacgatgcc cctgatgatc tcctttgcct tgttggccgc ggcttcgtcg gaggacgcaa
     1741 tgttgatgat gccgctgtct tcgatgtcga tcttggcgcc tgtctgctcc acgatgctcc
     1801 tgatcacctt gccgccgctc ccgatcacgt ccttgatctt gtccggattg atctgcatgg
     1861 tgatgatccg gggcgcaaag ggattgagcg tcttgcgggg ttctgccagg gcttcgagca
     1921 tcttcccgag gatgtgcagc ctgccgtcct ttgcctgttt gagggccgtc cgcatgacct
     1981 cgatagtgat gccttcgatc ttcatgtcca tctggagcgc cgtgatgccc ttgctcgtac
     2041 cggtgacctt gaagtccatg tcgcccagat ggtcttcgag gccgaggatg tcggaaagaa
     2101 tgatgacctt ctccccttcc ttgatgagcc ccatggcgat gccggcgacc ggcgccttga
     2161 tcggcacacc cgcgtccatg agtgccaggg tgccgccgca aaccgtcgcc atcgaggacg
     2221 atccgtttga ttcgaggatg tcggagacga ggcggatggt gtacgggaat tccagcttgg
     2281 ttgggaccat cgtcttcaag gcccgttccg caagggcccc gtgacccact tcacgccttc
     2341 caggtccacg caggggtttg gtttcaccca cgctgaaagg cgggaaatta tagtgtagca
     2401 tgaaggtctt gaaatattcc ccttcgagtg agtcgatccg ctgttcgtcc tcggaggtcc
     2461 cgagcgtggc cacgaccagg gcctgggtct cgccgcgggt aaagagagcg gagccgtggg
     2521 tccggggcag caggcccacg gaggagctga ttggcctgat ctcgtcgggt cttctgccgt
     2581 cagcgcggac attcttttcc aggataatct tccggacctc gtccttctcc aggtcgaaga
     2641 atatcgtcgc tatctgacgg cccctcgtcg gatcatcttc cttcttcagc ttctgtttga
     2701 tctcgtccaa aagcgcgtcc agcgtctttt gtcgctccat cttgttcgga ataacgatgg
     2761 cggtcagcaa acgttccatg gccagcgcgg ccacctggac ggccaggtcc ttgtcgatct
     2821 caacaacctt gacttgtcgt ttgggcaccc ctactttcgc tataagctcg ttttggagcg
     2881 caacgatctt cttaatttcg cggtgcgcgg tttcaagggc ctcgagcatc tggtcctcgg
     2941 acagctctgt cgccccgcct tccaccatca tgatagcgtc ggccgtgccg gccaccacga
     3001 ggttaagacg ggttgtttcg agttccttct ggccgggatt gatgacgaac ttgtcgtcaa
     3061 taaaacccac ccgcactgcc ccgatgggtc ccctgaaggg gatctcggat atggagaggg
     3121 ccgctgatga agcgatgatg cccatgagat ccatggagct ttcatcgccg atcgagagca
     3181 ccgaagcgat gatctgcgta tcgaagtaat atccctccgg gaacaacgga cgtatcggcc
     3241 ggtcgatgag gcgcgacgac agaatttcct tttcggtctg cctcccttcg cgcttaaaaa
     3301 atccgcccgg tatccttccg gctgcatagg ccttctcctg ataatctacg gtgagcggaa
     3361 gaaaatcgac gttctcacgc tccaccttcg aggcaacggc cgtcgacagg acgacggtgt
     3421 caccgtaccg ggcaacgacg gcgccgcttg cctgcttggc catgacccct gtttccagga
     3481 tcagccgttt gccgcctatt tctgtttcca ctgttgtt
//
LOCUS       DASSLD010000254         4615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1410537, whole genome shotgun sequence.
ACCESSION   DASSLD010000254 DASSLD010000000
VERSION     DASSLD010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410537"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VFK23_04600"
     CDS             complement(<1..741)
                     /locus_tag="VFK23_04600"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HET7318399.1"
                     /translation="MTLPAESQDTKRYLANWKEERDSAALYRIMADHEKNRKIAEVYR
                     KLAETEDTHAENWAKRLQEAGGDVPLYRPSLRTRVLMRLIRHFGVTAVVPALSRREIQ
                     GSRDYGRQPEAADMVRAEQSHARLLREITKTTQGGMEGSSLARLEGRHRSTGGNALRA
                     AVLGASDGLLSNFNLIAGAAGAELSNRNILFTGFAGLLAGGISMALGEWISVQSTREL
                     YQKQISSEKEEIAAAPEEEVEELVLIYQA"
     gene            complement(750..1253)
                     /gene="larC"
                     /locus_tag="VFK23_04605"
     CDS             complement(750..1253)
                     /gene="larC"
                     /locus_tag="VFK23_04605"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HET7318400.1"
                     /translation="MKKKARLLFAQADHVSGELTGFAVGKIMELGARNVQLISSVTKK
                     NRPGNIIIIDTDDDHEEAISRFLARELKISGYHRIETSHIFQQVTFSQKDLVIRTNGK
                     RRTFCCEVKIIGDPSDPLSVDVEHEALVKLQKVLKAQKKELSLSDLKTAIESRLRSSG
                     KKIVLEV"
     gene            complement(1262..3031)
                     /locus_tag="VFK23_04610"
     CDS             complement(1262..3031)
                     /locus_tag="VFK23_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318401.1"
                     /translation="MEKEIIAAFRAATAKPDYLTVDKLDAGLGGWGAFNLGVWAAANV
                     IAKEMKKGKHMKLEPSDEPHTDVDEIARKAVRKLMECGADPANAALSAAVLLYWSGVN
                     VQCGMPCPNRKLGAVTRMAAGIPSGRVSSMPTEKQNNKISGFAATLAIYNALDKEHLA
                     PYDSAFLPLGAGGPIIGHSAIGEDHLFPKLGQKLAGIGTKAMMKAYASAGMRPNKWFS
                     ALFGTTAALEIIHPDAYVGEEFGSFLVTRTPETTAQAAVEASGLPARIHIRGTGEELN
                     TAQVVGDLAIILKDSGTPTVVGMIMFAELCSIIQEGSAIGAGRSGGPLVIPLHHWVTS
                     PTLALYLQGKGASQEEIVAVISKTMDMHFQRDQAITATNLLARKAETVEKGPLTDAIL
                     AATEPGFTNAVATRAIYAYTEMKRGKTLQEVVREIQKKLIESLAGSVAKAMSKRLNRE
                     IEYIRFKEVRPGSGRRTHTFAQRHFAFDAYIDVEVKIDGKVYNLDNVLAHAAPEALMH
                     TDKETIDAISAVSPAIIDLLCSGATSLDTTVCACVAAAMGMDPKEAAKEAAAAANVML
                     ALPAPSLKKATQLASDIMKGLDF"
     gene            complement(3064..4101)
                     /locus_tag="VFK23_04615"
     CDS             complement(3064..4101)
                     /locus_tag="VFK23_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET7318402.1"
                     /translation="MNKPREAIFHALGGGKADRIPAALIGGGMWSVRDFGTSFRELST
                     DAPQMAGMITATADRLRSDIVYVGSGYPNFPVAALGGKIKYRDVGTPDLEAPIVSSEQ
                     DLAKLDLSLIEQDEVLRTIKEAYGMVRSKLGGEYVVTVTAWGPFTLGARLMGEEAMMK
                     ALYKAPSFVRAAVSVAAEMLMRFYDSLLSPGMLDVILLGEPTASGDLVSRKQFEQFVL
                     PPVKEFTGWAKGKGAHTILHICGNTTDRLDLYPLTGAGCISLDHKTDMAKAKEALRGK
                     MCFAGNVDPVKVLLQGTVREVEDACRNVIKTAGTDGGFILMPGCDIPPTVPYENIRTF
                     MQTAREWEWQE"
     gene            complement(4199..>4615)
                     /locus_tag="VFK23_04620"
     CDS             complement(4199..>4615)
                     /locus_tag="VFK23_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7318403.1"
                     /translation="TLEVLESAIMAYPERTLDNIKRLDATGVLLSVDDFGTGYSSLSH
                     LQKLPVDEMKIDKSFVYDMDRSANDATIVRSMIELAHNLGLKVTAEGVETQQVWDMLK
                     SHGCDLAQGYLMSRPMTVDDLVRWAKESAWGLGKEG"
BASE COUNT          832 a         1366 c         1333 g         1084 t
ORIGIN      
        1 ggcctggtaa attaaaacca gttcttcgac ctcttcttcg ggcgcggcgg caatctcctc
       61 cttttcgctg ctgatctgtt tctggtacag ttcacgcgtg ctctggaccg agatccactc
      121 gccgagtgcc atcgatattc ctccggcaag caggcctgcg aagccggtga acaggatgtt
      181 cctgttggag agttcagccc ctgccgcgcc cgcgatgagg ttgaagttgg aaagcagacc
      241 gtcgcttgcg cccagcactg ccgcgcgcag ggcgtttcca cccgtggacc gatgacggcc
      301 ctcaagccgc gccagcgagc tcccctccat ccccccctgg gttgtctttg ttatttcccg
      361 caggagccgg gcatgggatt gttccgcccg gaccatgtcg gcggcttcag gctgcctgcc
      421 gtaatcgcgg gagccttgta tctctctcct cgaaagcgcg ggaacaacgg cagttacgcc
      481 aaaatgtcga atgagccgca tgagcacgcg cgttcgaagc gagggacggt acagaggcac
      541 gtcgcctccg gcttcctgta atcgcttcgc ccagttctcg gcatgcgtat cttcggtttc
      601 ggccagtttc cggtaaactt cggcaatctt ccggtttttt tcgtgatccg ccattatccg
      661 gtagagcgcc gcgctgtcgc gttcttcttt ccaattggca agatagcgtt tggtgtcctg
      721 tgactcagcc ggtaatgtca tgatatgcgc tagacctcca ggacaatttt ttttccggac
      781 gaccgcagcc tggactcgat cgccgtcttc aggtcgctga gggaaagttc ctttttttgc
      841 gccttgagga ctttctgcag cttcacgaga gcctcgtgct cgacatcgac ggacaggggg
      901 tcagatgggt cgccgatgat cttgacctcg cagcaaaacg tccgtctttt cccgttcgta
      961 cggataacga ggtccttttg cgaaaaggtg acttgttgaa aaatatggct ggtctcgatc
     1021 ctgtggtatc ccgatatctt gagctccctg gcgagaaacc gggagatcgc ttcttcatgg
     1081 tcgtcgtcgg tgtcgatgat gatgatgttg ccgggacggt ttttcttggt aacggaggag
     1141 atgagttgaa cattccgcgc cccaagctcc atgatctttc cgacggcaaa tcccgtcagt
     1201 tcaccggaaa catggtctgc ctgggcaaag aggaggcggg ccttcttttt catgtccgtt
     1261 ttcaaaaatc gagtcctttc atgatatcgg aggccagttg agtggccttc ttgaggctgg
     1321 gcgccggcag cgcgagcatc acgttggccg cggcggccgc ctctttcgcc gcctctttcg
     1381 ggtccatacc cattgcggct gccacgcagg cgcaaacggt cgtatcgaga gaggttgcac
     1441 ctgagcacag aaggtcgatg atggccgggg agacggcaga gatggcgtcg atcgtctcct
     1501 tgtcggtatg catgagggct tcgggcgcag catgcgccag cacattatcg aggttgtaga
     1561 cctttccgtc tattttcacc tccacgtcga tgtaggcgtc gaaggcaaag tgacgttggg
     1621 caaaggtgtg ggtcctcctg cccgacccgg gacggacctc cttgaaacgg atgtattcga
     1681 tttcccggtt gagcctttta ctcatggcct tcgcgaccga accggcaagc gattcgatca
     1741 gttttttctg gatttcacgg acgacctcct ggagggtttt cccccgcttc atttcggtat
     1801 atgcatagat cgccctcgtg gcaactgcgt tcgtaaaacc cggctcagtt gcagcgagaa
     1861 tcgcatcggt gagcggcccc ttctcgactg tctcggcctt gcgtgcgagc aggttcgtcg
     1921 cggtgattgc ctggtcgcgc tgaaagtgca tgtccatggt cttgctgatg acagcgacga
     1981 tctcctcctg ggaagctcct ttcccctgca ggtagagggc aagggtggga gaagtaaccc
     2041 agtggtgaag cggtatcacg agcggtcctc ccgatctccc cgcgccgatt gcggacccct
     2101 cctggatgat ggagcagagt tcggcgaaca tgatcatgcc gacgaccgtc ggggtgccgg
     2161 agtccttaag aatgatcgca aggtctccga caacctgggc tgtattcagc tcttcacctg
     2221 ttccacggat atggatcctg gccggcaagc cggaagcctc gacagcggcc tgggccgtcg
     2281 tttccggcgt tcgagtgacg agaaagctgc cgaactcctc tcccacgtag gcatcggggt
     2341 gaatgatctc gagcgcagcc gttgttccga acagcgcgga gaaccattta ttgggccgca
     2401 tgcccgcgga ggcataggcc ttcatcatgg cttttgtccc gatcccggcg agtttttgtc
     2461 ccagcttcgg gaacagatga tcttcgccga tggctgaatg gcctatgatg gggccgcccg
     2521 cgccgagggg aagaaatgcc gagtcatagg gggcgagatg ctccttgtcg agggcgttgt
     2581 agatcgcgag cgtggcggca aagccggata tcttgttgtt ctgtttctcc gtgggcatgg
     2641 aagagacgcg gcccgagggt atgcccgcgg ccattctcgt taccgcgccg agtttccggt
     2701 tcggacaggg catgccgcac tggacgttta ctccggacca atacaggagc accgctgccg
     2761 agagcgcggc atttgccggg tccgctccgc actccatgag cttcctgacc gcttttcgcg
     2821 cgatctcgtc cacatcggtg tggggttcgt ccgagggctc cagcttcatg tgcttgccct
     2881 tcttcatctc ttttgcgatg acgttggcgg cggcccagac gccgagattg aaggcccccc
     2941 atccgccgag gccggcgtcc agcttgtcca cggtcaggta atcgggcttt gccgtggccg
     3001 ccctgaatgc cgcaatgatt tctttctcca tgtttgactc cttgtgaggg cgtttgagtc
     3061 aggtcattcc tgccattccc attcccgggc cgtctgcatg aacgttcgga tattctcata
     3121 ggggacggtg ggcgggatgt cgcagcccgg catcagaatg aatccgccgt cggtgcccgc
     3181 agtcttgatc acgttcctgc aggcatcctc cacttcccgg accgttccct gaagcaggac
     3241 cttcaccggg tccacgtttc ccgcaaaaca catctttccg cgaagcgctt cctttgcctt
     3301 tgccatgtcc gtcttatggt cgaggcttat gcagccggcg ccggtcagcg gatagaggtc
     3361 cagcctgtcg gtggtgttgc cgcagatatg gagaatcgta tgggcgccct tcccttttgc
     3421 ccagcccgta aattccttca ccgggggaag gacaaattgt tcgaactgct tccgcgaaac
     3481 aaggtctccc gaagcagtcg gttcgcccag caggatgacg tcgagcatgc ccggcgacag
     3541 gagtgaatca tagaaacgca tgagcatctc agcggcaacg ctcactgccg cccggacaaa
     3601 agaaggcgct ttgtagaggg ccttcatcat ggcttcttct cccatgagcc ttgccccgag
     3661 ggtaaagggg ccccacgcgg taacggtaac gacatattcc ccgccgagct tggacctgac
     3721 catgccgtaa gcttccttga tggtccggag cacttcgtct tgttcgatca gcgacaggtc
     3781 gagtttcgcg aggtcttgtt ctgacgaaac gatgggagct tctagatcag gggtgccgac
     3841 gtcgcggtat ttgatcttcc ctcccagcgc ggcaacggga aagttgggat atcctgaacc
     3901 gacgtaaacg atgtcgctgc gcagcctgtc cgccgtcgct gtgatcatcc cggccatctg
     3961 cggagcatcg gtggagagtt ctctgaaaga agtgccgaag tcgcggacac tccacatgcc
     4021 gccgccgatg agcgccgccg gaatcctgtc cgctttccct ccgccaaggg catggaatat
     4081 ggcttcccgg ggtttattca tctttgcggt cctcgtcgtt taccgcctgt caaaacaata
     4141 tcatgaagac attgtacggt ttgtgtgccg cggcgaaatc ctattccgta ttctcccctc
     4201 atccttcctt cccaagcccc caggccgact ccttcgccca ccggaccaga tcatcgaccg
     4261 tcatgggcct gctcatgagg tacccctggg ccaggtcgca gccatgggac ttgagcatgt
     4321 cccatacctg ctgggtttcg acgccttccg cggtgacttt caatcccaga ttgtgcgcca
     4381 gctcgatcat cgagcgaacg atcgttgcgt cgttcgcgct cctgtccatg tcatacacga
     4441 acgacttgtc gatcttcatc tcgtccacgg gaagcttctg gagatgcgac agtgaggaat
     4501 aacccgtgcc gaaatcatcg acggaaagta gcacccccgt ggcatcaagg cgtttgatat
     4561 tgtccagcgt acgctccgga taagccatga tcgcgctttc caggacctcc agggt
//
LOCUS       DASSLD010000255         2517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1208192, whole genome shotgun sequence.
ACCESSION   DASSLD010000255 DASSLD010000000
VERSION     DASSLD010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208192"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VFK23_04625"
     CDS             complement(<1..766)
                     /locus_tag="VFK23_04625"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7318404.1"
                     /translation="MSGMKELDRHVWSRIAEVVQQNRKSVIIAAGVLAAAAAALLFTG
                     SCSQREHAAAVHREEDHQEKQSVVKLSAEAQKTAGITVRTAAPEPLSAPIEATAAVEL
                     NGDKVANVSARTSGRLVRITALQGDVVKENQPLAYFDSPELGQAWAEYSKAKGRVEAS
                     RKNVSREETLFEKKISPEKDVIRARQELAEAEADLSFAREKFHLLGVDIDRFEMKREG
                     EHQHPLIAIASPISGTVIERTATQGEVVGPEKAIFTI"
     gene            complement(763..1989)
                     /locus_tag="VFK23_04630"
     CDS             complement(763..1989)
                     /locus_tag="VFK23_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7318405.1"
                     /translation="MTRMITFACSLVLFAHPVLAVTLGEAVDTALKNNPDLQSVRLEK
                     DAAQGRLDQAGLWLRSNPVIEGSLSRKDTVPGGGEKVTNHTFALSQEFEIAGQRGLRI
                     DAARGGMEKANQEIRDRERVLIADVKDAFAKALAAKRKVELASEAVRLQEQLAGSAMI
                     KFQAGEISQLEQNLADVELGKARREHLLAEKERREALLGLQALLGSPPKSDFSVEGDL
                     PIEPPSIPDKEKLRTLTASQRPDVLSASAEVQQARSAMKLAGREAIPSFTITGFYNQD
                     DKLNETGVMLSFPLPLFDRKQADRKEAAAKAGQALIKQASLERTLEREIEEAFANLVS
                     ASEELSLFKKDVLGKALENLSLMNLAFKEGKFGFFEVRLAQKDTIDAQFAYLESQVRL
                     QIALNAMEKVTGGSLR"
     gene            complement(2102..>2517)
                     /locus_tag="VFK23_04635"
     CDS             complement(2102..>2517)
                     /locus_tag="VFK23_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318406.1"
                     /translation="EDVTGLFSPCLMKKIPVICFTMQFRAFLIMLLLAMVVAAPVVDA
                     TICEDCNHAAPVLDPSQRLIDPAGSAATISGAPGTDSPGTESAQDLCPFCSHSVAATV
                     SPACYIPSTISNAFPLPKLLALFNLSKSITKPPQN"
BASE COUNT          484 a          771 c          696 g          566 t
ORIGIN      
        1 cgattgtgaa gatggccttt tccggcccca ccacctcgcc ctgggtcgcc gtccgctcga
       61 tcaccgtgcc gctgatgggc gaagcgattg cgatgagcgg gtgctggtgc tcgccctcgc
      121 gcttcatctc gaaccggtcg atatcgaccc cgaggaggtg gaacttctca cgggcgaagg
      181 acaggtccgc ttcggcctcg gcaagctcct gccgggcacg gatcacgtcc ttttccggag
      241 agatcttctt ctcgaaaagc gtttcctccc gactgacgtt tttccgtgag gcctcgaccc
      301 tgccttttgc cttggaatac tcggcccatg cctggcccag ctcggggctg tcgaagtacg
      361 caagaggctg gttctccttc accacgtcgc cctgtaacgc cgttatcctg acgagcctgc
      421 ctgaggtccg cgcgctcaca ttcgcgacct tgtccccgtt cagctcgacc gcggcagtcg
      481 cctcaatggg agcggaaaga ggctccggcg cggcggtccg gaccgtgatc cccgcagtct
      541 tctgcgcttc cgccgagagc ttcacgacgc tctgtttttc ttgatgatcc tcttccctgt
      601 gaaccgcggc cgcatgctcc cgctgtgagc atgatccggt aaagagcagc gcagcagcgg
      661 ccgccgcaag gacaccggcc gcgatgataa ccgacttcct gttctgttgt actacctcag
      721 cgatccggct ccatacatgc cggtccagtt ctttcatccc gctcatctca gacttcctcc
      781 tgtcactttc tccattgcgt tcaacgcgat ctgcagccgc acctgtgatt ccaggtaggc
      841 gaactgcgcg tcaatcgtat ccttttgcgc gagccgtacc tcgaagaacc cgaacttccc
      901 ctccttgaat gcgaggttca tgagactcag attctccagt gccttgccga gcacatcctt
      961 cttgaagaga gaaagctcct cggacgcaga cacgagattc gcaaaggcct cctcgatctc
     1021 ccgctccaag gttcgctcca gcgacgcctg cttgatgaga gcctggcctg cctttgcggc
     1081 ggcttctttc cggtccgcct gcttccggtc gaataacggc agcgggaagg acagcatgac
     1141 gcccgtttcg ttcagtttat cgtcctggtt gtaaaatcct gtgatcgtga agctcgggat
     1201 agcctccctg cctgcaagct tcatggctga tctggcctgc tgcacctccg ctgacgccga
     1261 cagaacgtcc ggacgctgtg aagcggtaag cgtccgaagc ttttctttgt ccgggatcga
     1321 tggaggttca atcggcaaat ctccttccac ggagaaatcg gatttcggcg gtgatcccag
     1381 aagagcctgt aagcctagca gagcttctcg acgttccttt tcggcaagca gatgttccct
     1441 cctggctttg ccgagttcaa catcggccag attctgttcg agctgggata tctctcccgc
     1501 ctggaacttg atcatggccg agccggcaag ctgttcctga agacgtaccg cctcggaagc
     1561 aagttctact tttcgcttcg cagcaagcgc ttttgcaaaa gcatccttta catcggcaat
     1621 caggacgcgc tcccggtcac gaatttcctg attggccttc tccatgccgc ctcgggcggc
     1681 atcgatccgt aatccccgtt gtccggcgat ttcgaattcc tgggaaagtg caaacgtatg
     1741 gttggtcacc ttttctcctc cgccaggtac ggtgtctttt ctcgacaggc tgccttcaat
     1801 cacgggattg cttctgagcc aaagccccgc ctgatcgagc ctgccctggg ccgcgtcctt
     1861 ctccagcctc acggactgaa ggtcgggatt gttcttgagc gccgtgtcca cagcttcgcc
     1921 cagagtgacg gcaaggaccg ggtgcgcaaa tagtactaga ctgcacgcaa acgtaatcat
     1981 tcgtgtcatg aatacacctc gcttggctgc aatgaatgag ggaaaagcgc gagcgcgcga
     2041 caacgcacgg catgacgcag gtcacgcgga cgcaggcggc tgcatcgtga agctgagggg
     2101 gttagttttg aggcggcttc gtgatggact tcgagagatt aaacaaagcg agcaacttcg
     2161 gaagagggaa cgcattgctg atcgtggacg gaatgtagca ggccggcgat actgtggctg
     2221 cgacgctatg ggaacagaac ggacaaaggt cctgcgccga ctccgttccc ggggaatccg
     2281 tgcccggtgc gccggaaatc gtggcggcag aacctgccgg atctatcagg cgctgcgacg
     2341 ggtctaaaac aggagctgca tggttgcaat cctcgcagat tgtcgcgtcg accacgggtg
     2401 ccgcgacaac catcgccaaa agcaacatta tgaggaatgc cctgaactgc atagtgaaac
     2461 atattacggg aatttttttc attaggcaag gggaaaacaa gcccgtaacg tcttctc
//
LOCUS       DASSLD010000256         5256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_201201,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000256 DASSLD010000000
VERSION     DASSLD010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201201"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1871)
                     /gene="fusA"
                     /locus_tag="VFK23_04640"
     CDS             complement(<1..1871)
                     /gene="fusA"
                     /locus_tag="VFK23_04640"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET7318407.1"
                     /translation="MKNDDVKEVRNIAILSHGAAGKTSLADAMVYAGGAVDIMGSVDN
                     GTSVFMHEPEEMSRKITITSSLGFTDWKGVRINIIDTPGYINFLEETKGTLRAADGAV
                     IIVSAISGVKAETEKIYKFACDYEISRIAFISKLDKERADFFRAVGDMEKYFCKGALV
                     LQLPIGLEEDFSGVVDLIRMKALYFAKDGSGKTEEKEIPDAMKADAAAYRKKLVEQIA
                     ETEDSLLEKYLDKGDLSHEELITGLKHGTLVGGLLPVLCGSPVRNMGIQQLMDAVVTC
                     LPSPVERAGGVAVRGKDPKTGQEIVRKPVPEEPLAALVFKTVIDPFAGKLSMVRVFSG
                     TLKADSTVYNSTRQTKEKIGSLFLVQGKKQVSVPALHAGQIGAVAKLKETQTGDTLCV
                     EHPAIVMDFAKFADPVMSYAVAPKTRGDEDKVSIGLHKLLEEDPTLKFAYDDQTKEMV
                     LSGMGQVHLEVTLEKLKRKFGADVTMKTPKVPYKETIRAKASAQGKYKKQTGGHGQYG
                     DAWLEIEPLPRGSGFEFVDNIVGGVVPRQYIPAVEKGVVEALHEGVLAGYPVVDIRVS
                     LYDGSYHTVDYSEMAFKVAASMAFKKAVEAAKPVLLEPIMSVEVISPDDTLGAVIGDL
                     "
     gene            complement(2084..2350)
                     /locus_tag="VFK23_04645"
     CDS             complement(2084..2350)
                     /locus_tag="VFK23_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318408.1"
                     /translation="MRLIKNPDEARRLARTILSDILLYNQAKVKEGIEQDSLFDVLTE
                     ELAEGRKYYESMVDEEIRRASNYFNEAVVDVLIKQGGKIKSDIW"
     gene            complement(2364..2834)
                     /locus_tag="VFK23_04650"
     CDS             complement(2364..2834)
                     /locus_tag="VFK23_04650"
                     /inference="COORDINATES: protein motif:HMM:NF016160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HET7318409.1"
                     /translation="MRFALCAMRCLGGIMIEEEGVVAEVEGNIAKVAVLKKSACEQCA
                     AAGVCHPGDQEYLEAANPLNAVKGQKVKVVIAPQMYLKASLILYGIPMTALIAGAIIA
                     KNIAVPYVGEERSDLWAFIAGMACTVISFVFIRSYNKKVEKTQKYKPVIVEILS"
     gene            complement(2878..4473)
                     /gene="cimA"
                     /locus_tag="VFK23_04655"
     CDS             complement(2878..4473)
                     /gene="cimA"
                     /locus_tag="VFK23_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HET7318410.1"
                     /translation="MKSKVQLYDTTLRDGAQAEDVNFSVEDKIRIARKLDQFGVHYIE
                     GGWPGSNPRDVEFFGEMRRVKLKRAKLAAFGATRRAKLRVSDDPSIKSLVASGAPVAT
                     IFGKTWDLHVHKALRTTLEENLAMISDSVAYLKKRLDEVVYDAEHFFDGYKANPEYAT
                     LTLLAAENAGADCIVLCDTNGGTLTHEVEEIIQQVKRRIKTPFGIHAHNDAELAVANS
                     LAAIRMGAVQVHGTVNGYGERCGNANLCTIIPSLKLKMGIDCVSDGNLAKLRDLSRYV
                     DELANLPHRKRQPYIGDSAFAHKGGVHVDAIAKSALTYEHIAPELVGNRRRILVSDMA
                     GKSNILQKAAELGTPLTKDSPELAGILRKVKELENEGYEFEGAEGSLELLMLRAGHNY
                     ESVFSMFDRIDYRVLTEKRKVDPHPVSEATITVEVGGNVEHTAAWGNGPVNALDKALR
                     KVLPGYFPGRGLENVRLIDYKVRVLTAAEGTAARVRVLIESGDGRNKWGTVGVSENVI
                     EASWQALVDSIEYKLLRSKKKSA"
     gene            complement(4607..>5256)
                     /locus_tag="VFK23_04660"
     CDS             complement(4607..>5256)
                     /locus_tag="VFK23_04660"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET7318411.1"
                     /translation="LDKISYDEMLELASLGAKVLQTRSVEFAKKYNVPVRVLSSFNDN
                     PGTLVTKEDSDMEKVVVSGVAYDKNQVKVTVQGVPDKPGVAARIFNTVSDNNVVVDMI
                     IQNIGEGGLTDMSFTVPKTDSKKIMEVMKKVVTEIGAKDVNIQDDIAKISIVGVGMRS
                     HSGVAAKMFSALAKEGINIMMISTSEIKISCVIDAKYTELAVRVLHDAFEMEKAA"
BASE COUNT          957 a         1620 c         1459 g         1220 t
ORIGIN      
        1 aggtcgccga tcacggcgcc gagcgtgtcg tcgggagaga tcacctcgac gctcatgata
       61 ggctcgagga ggaccggttt ggccgcttcc acggccttct tgaaggccat gcttgccgcg
      121 accttgaacg ccatttctga atagtccacc gtatggtagg agccgtcata cagcgagacc
      181 ctgatgtcca cgacgggata ccccgcgagg acgccctcgt gcagggcctc gacgactccc
      241 ttctcgacag cggggatata ctgccggggg accacgccgc ctacgatgtt gtccacgaat
      301 tcgaacccgc tcccgcgtgg cagaggctcg atctcaagcc aggcgtcacc gtactggcca
      361 tggccgcccg tctgtttctt gtacttgccc tgtgcgctcg ccttggccct gatggtctcc
      421 ttgtacggga ccttcggcgt cttcatggtc acatccgcgc cgaatttccg cttgagcttc
      481 tcgagcgtca cctcgaggtg gacctgcccc atcccggaaa gcaccatttc cttcgtctga
      541 tcgtcatagg cgaacttcag ggtgggatct tcctcgagga gcttgtgaag gccgatgctc
      601 accttgtcct cgtccccccg ggtcttggga gccacggcgt aggacatcac ggggtccgcg
      661 aatttcgcga agtccatgac gatcgccggg tgctcgacgc acaacgtgtc gccagtctgc
      721 gtctccttga gttttgccac ggcgccgatc tggcccgcgt gaagggccgg tacggacacc
      781 tgcttcttgc cctggaccag gaacagggaa ccgattttct ccttggtctg ccgggtggaa
      841 ttgtagaccg tggagtcggc cttgagcgtc cccgaaaaga ccctcaccat ggaaagctta
      901 ccggcaaagg gatcgatgac ggtcttgaaa acgagcgcgg caagcggctc ttcggggacg
      961 ggcttgcgca cgatctcctg gccggtcttc gggtccttgc ccctgacggc cacaccaccc
     1021 gcccgttcca ccggcgaagg caggcatgtg accacggcgt ccatgagctg ctggatcccc
     1081 atgttcctta cgggggaacc gcagaggacc ggcaggagtc cgccgaccag cgtgccatgc
     1141 ttgagccccg tgatcagttc ttcgtgcgag aggtcaccct tgtcgagata tttttcgagc
     1201 agactgtctt ccgtttccgc aatctgctcc acgagcttct tgcgataggc ggccgcgtcg
     1261 gccttcatgg cgtcggggat ctctttttcc tccgttttgc cgctgccgtc cttggcgaag
     1321 tagagggcct tcatcctgat gaggtcgacg acgccgctga agtcctcttc caacccgatg
     1381 gggagttgaa ggaccagggc gcctttgcag aaatacttct ccatgtcgcc cacggcgcga
     1441 aagaaatcgg cccgttcctt gtcgagcttg ctgatgaagg cgatgcggga gatctcgtag
     1501 tcgcaggcga atttatatat tttttcggtt tcggccttga cgcccgaaat ggcggagacg
     1561 atgataacgg cgccgtcggc ggcgcgcagg gtccctttgg tctcttcgag aaagttgatg
     1621 tagcccggag tgtctatgat gttgatccgc acgcccttcc agtccgtgaa gcccagggaa
     1681 gaggtgatgg tgatcttcct cgacatttcc tcgggctcgt gcatgaaaac cgatgtgccg
     1741 ttatcgacgc tgcccatgat gtcgacggcg cccccggcat agaccatggc gtcggcaagc
     1801 gaggtcttgc cggccgcccc gtgtgacagg atggcgatgt tgcggacttc cttcacatca
     1861 tcatttttca taagacgctc ctttgagtga taggtagaac accgtgtatc ttatgaacag
     1921 tctcagaaca ggaacaacag gatcgtgatt caggaagctg tctttcccga gtatgaaatc
     1981 agagccctga cgctgaacca tatccccgat aagggcacgg gggatggcaa gcgtttgctg
     2041 ccggagtttc tgaaaagact atggcgagac gattactatg agtttaccag atatcggact
     2101 ttattttccc tccctgtttg atcaggacgt cgacgacggc ctcgttgaaa tagttgcttg
     2161 cgcggcggat ttcctcgtcc accatgcttt cataatattt gcggccttcc gcaagctcct
     2221 ccgtcaggac gtcgaacagg ctgtcctgct cgattccctc cttcaccttc gcctggttgt
     2281 acagaaggat gtccgacagg atcgtacgcg cgagccttct cgcctcatcg gggttcttga
     2341 tcagtctcat atcagacgtc cgctcaggaa aggatctcca cgattaccgg tttgtacttc
     2401 tgggtcttct cgaccttttt attataggac cgaatgaaca cgaaggaaat aacggtgcac
     2461 gccatacctg caatgaacgc ccatagatcg gaccgctcct cgccgacgta gggcacggcg
     2521 atatttttcg cgatgatggc gccggctatc agagcggtca tggggattcc gtaaaggatg
     2581 agggacgcct tgaggtacat ctggggcgcg atgaccacct tgaccttctg tcccttcacg
     2641 gcgttcaaag ggttcgcagc ctccaggtac tcctggtcgc caggatggca cacaccggcg
     2701 gcggcgcact gttcgcacgc gctctttttg aggaccgcca ccttggcgat gttgccttcc
     2761 acctcggcga cgaccccttc ttcctcgatc atgatcccac ccaagcaccg catggcgcag
     2821 agcgcaaagc gcatggcgtt atgctcgcct tccgcgccat gctctctgcg ctatgcccta
     2881 tgcgcttttc ttcttcgacc tcagcagctt gtactcgatg ctgtccacca gcgcctgcca
     2941 gctcgcctcg atcacgttct cggacacacc cacggtgccc catttattcc tgccgtctcc
     3001 cgattcgatc agcacgcgca cccgggcggc cgtcccctcg gccgccgtca gcactcgtac
     3061 tttgtagtcg atcagccgta cgttttcgag ccccctcccc gggaaatacc ccggcagcac
     3121 cttgcgaagg gccttgtcca gggcgttcac gggaccgttg ccccaggcgg ccgtatgctc
     3181 gacgttcccg cccacttcga ccgtaatggt cgcttcgctc accgggtgcg ggtcgacttt
     3241 ccgcttctcc gtcaaaacgc ggtaatcgat ccggtcgaac atgctgaaca ccgactcgta
     3301 attgtggccc gcgcggagca tgagaagttc gagcgagccc tcggcgccct cgaactcata
     3361 accctcgttc tcgagttctt tgaccttcct gaggatgccg gcgagttcgg ggctgtcctt
     3421 cgtgagcggt gtgcccagct ctgcggcctt ctgaaggatg ttgctctttc ccgccatgtc
     3481 cgacacgagg atgcgccgcc tgttcccgac gagttcaggg gcgatatgtt cataggtcag
     3541 ggcgctcttt gcaatggcat ccacgtgcac cccgcccttg tgcgcaaaag cgctgtcgcc
     3601 gatgtagggc tgccgcttgc ggtgaggcag gttcgcaagt tcatccacat accgggacag
     3661 gtccctcagc ttcgcaagat tgccgtcgga gacgcaatcg atccccatct tgagcttcag
     3721 ggacgggatg atggtgcaga gattggcgtt cccgcaccgc tcgccgtagc cgttcaccgt
     3781 tccgtggacc tgcacggccc ccatcctgat cgcagcgagc gagtttgcca cggccagttc
     3841 cgcgtcgtta tgtgcatgaa tcccgaaggg ggttttgata cgcctcttta cctgctgtat
     3901 aatctcttcg acttcgtgcg tcagcgtacc gccgttcgta tcgcataata cgatacaatc
     3961 cgcgcccgcg ttctccgccg cgaggagcgt aagggtcgca tactcgggat tggccttgta
     4021 tccgtcgaaa aaatgttctg cgtcgtagac aacctcatcc aggcgcttct tcaggtacgc
     4081 caccgaatcg gaaatcattg caagattttc ctcgagcgtg gtcctgagcg ccttgtgcac
     4141 gtgaaggtcc cacgtctttc cgaagatggt tgcgaccgga gcgccggacg cgacgaggga
     4201 tttgatgctc gggtcgtcgg agaccctgag tttcgcgcgg cgcgtggcgc cgaaagcggc
     4261 gagcttcgcc cttttcagtt tgacgcggcg catctctccg aagaactcga cgtcgcgcgg
     4321 gttggaaccc ggccagccgc cctcgatata gtggacgcca aactggtcga gctttcgagc
     4381 aatgcggatc ttgtcctcga ccgagaagtt aacatcctcc gcctgggccc cgtcacggag
     4441 ggtggtatcg tagagttgga cttttgattt catgacgcgt ggtgtttttc cgctaacact
     4501 tctcttgagc tcaatgcgga attcggaatg cggaattcgg aataaaaaaa ccgcatattg
     4561 taagataatt tacattccgc attccgcatt cgccattccg aattcgtcac gccgccttct
     4621 ccatctcgaa cgcatcgtgg aggacccgga ccgcgagttc cgtatacttg gcgtcgatca
     4681 cgcaggagat cttgatctcc gaggtgctga tcatcatgat gttgatgcct tcctttgcca
     4741 aggcggagaa catcttcgcg gccacgccgg agtgcgaccg catgccgacc ccgacgatcg
     4801 agatcttcgc gatgtcatcc tgaatattca cgtctttggc cccgatctcg gtcacgacct
     4861 tcttcatgac ctccatgatc ttcttggaat cggtcttggg cacggtgaag gacatgtccg
     4921 taagtcctcc ttcgccgatg ttctgaatga tcatgtccac cacgacgttg ttgtccgaaa
     4981 cggtgttgaa gatccttgcc gcaacgcccg gcttgtccgg aacaccctgc accgtcacct
     5041 tcacctggtt cttgtcgtac gccacgccgg acaccaccac cttctccata tcggaatcct
     5101 ccttggtcac gagggtgccc ggattatcgt tgaagctcga gagcaccctc accggaacgt
     5161 tgtatttttt cgcgaactcc accgaacggg tctgcagcac cttggccccg aggctcgcca
     5221 gttcgagcat ctcgtcatag gaaatcttgt cgagct
//
LOCUS       DASSLD010000257         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_563188,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000257 DASSLD010000000
VERSION     DASSLD010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563188"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /gene="rsxC"
                     /locus_tag="VFK23_04665"
     CDS             <1..1241
                     /gene="rsxC"
                     /locus_tag="VFK23_04665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HET7318412.1"
                     /translation="ASLPKRAIIPLSQHIGAPTKALVVIGQEVKKGEKIGETTGFVSA
                     PVHASISGKVVALGSFPHILGMDIPAVVIESDGKDEWVGGLKENPDYMMLSSDELKKL
                     VQDAGIVGMGGATFPTHVKLSPPKEKPIDVVILNGAECEPYLTSDHRLMLEKSKEIVE
                     GLKILMRILNVSRGYIGIEANKPDAIESMTKAAAGSPEVKVWPVKVKYPQGAEKMLIK
                     AIAGRTVPAGALPMDVGVVVQNVGTAEAIYNAVRYSKPLIERYVTVTGRGVREPKNFL
                     ARIGTPFSQMIEEAGGLTDDAAKVIAGGPMMGMSQYTLDVPVIKGTSGITVLPKNEVS
                     TNSYGPCIRCGRCIDACPMKLQPSYIGLYIEKGHYEDAKDYNLMDCFECGSCTFVCPA
                     NRPMVQWVKKAKRELAKKKN"
     gene            1317..>2110
                     /locus_tag="VFK23_04670"
     CDS             1317..>2110
                     /locus_tag="VFK23_04670"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HET7318413.1"
                     /translation="MEAPAQVQPVQPKEPPKLIVSIGPHMHDLESTAKIMWTVSGALL
                     PATLMSVYYFGMPAIMVILVCLATSLLAEAGMQWLLKKPITLSDGSAFLTGLLLAMNL
                     PANAPLYIPFVASIVAIVIAKHLFGGLGYNIFNPALVGRAFVLVSFAKIMTTYVSPAA
                     TFMALDAKTTATPLVLLKEEGMAKLLEIYHTKAALYQDLFIGNRAGSLGETSVIALLL
                     GAAFLMMKRYITWHIPIPFILTVGVVTWLFGGKEGLMTGDPIFHMMS"
BASE COUNT          456 a          644 c          611 g          399 t
ORIGIN      
        1 ccgcatccct tcccaaaaga gcaatcatcc ccctcagcca gcacatcgga gccccgacca
       61 aggcgctcgt cgtcatcggg caggaagtca agaagggcga gaagatcggt gagaccacgg
      121 gcttcgtatc cgcgcctgtc catgcgtcga tctccggcaa ggtggttgcc ctggggagtt
      181 tcccgcacat cctcggcatg gatattcctg cggtcgtgat cgaaagcgac ggcaaggacg
      241 agtgggtcgg cggtctcaag gaaaatcccg attatatgat gctcagctcc gacgagttga
      301 agaaactcgt acaggatgcc ggcatcgtag gtatgggtgg cgccacgttc cccacccatg
      361 tcaagctttc accgcccaag gagaagccga tcgatgtcgt gatcctgaac ggcgctgaat
      421 gcgagcccta tctgacctcg gaccaccgcc ttatgctcga gaagtcgaag gagatcgttg
      481 aaggcctcaa gatcctcatg cggatcctga atgtgagcag gggttatatc gggatagaag
      541 ccaacaagcc cgatgccatc gagtcgatga ccaaggctgc tgccggttca ccggaggtca
      601 aagtatggcc ggtcaaggtc aagtacccgc aaggcgcgga aaagatgctc atcaaggcta
      661 ttgcgggcag aacggtgccg gccggagccc tgcccatgga tgtgggcgtg gtcgtgcaga
      721 acgtcggcac ggccgaggcc atctacaacg cagtccgcta cagcaagcct ctcatcgagc
      781 gctatgtgac cgttaccggc aggggcgtca gggagccgaa gaacttcctc gcccgcattg
      841 gcacgccgtt ctcccagatg atcgaggaag cgggcggact gaccgacgat gcggcaaagg
      901 tgatcgccgg cggccccatg atgggcatga gccagtatac tctcgacgtt cccgttatca
      961 aggggacatc gggcattacg gtccttccca agaatgaagt cagtacgaat tcctatggtc
     1021 cctgcatccg ctgcggccgg tgcatcgacg cctgccccat gaaactccag ccatcctaca
     1081 tcggacttta catcgagaag ggacattacg aagacgccaa ggactacaac ctgatggact
     1141 gctttgaatg cggctcctgc accttcgtat gccccgccaa ccggcccatg gtgcagtggg
     1201 tgaaaaaagc caagagggaa ctggcgaaga agaaaaacta acgggcaggg agcatagggc
     1261 gacgagcaga gggttcgact ccgtgccccg tgcaccatgc aagaggagtg atgttcatgg
     1321 aagcccccgc acaggtacaa ccggtacaac cgaaagaacc acccaaactg atcgtctcga
     1381 tcggccctca catgcacgac ctggagagca cggccaagat catgtggacg gtaagcggcg
     1441 cgcttctgcc cgccacgctc atgtcggtgt attatttcgg catgccggcc atcatggtca
     1501 ttctcgtctg tctcgcgacg tcgcttctgg ccgaagccgg gatgcagtgg ttgctgaaga
     1561 agcccattac gctttcggac gggagcgcgt tcctgacggg ccttctcctt gcgatgaacc
     1621 tgcccgccaa cgcgccgctc tacatcccgt tcgtcgcgtc catcgtggcc atcgtgatcg
     1681 ccaagcacct cttcgggggg ctcggctaca acatcttcaa cccggcgctc gtcggccgcg
     1741 ccttcgtcct ggtcagcttc gcgaagatca tgaccaccta tgtgtcgccg gccgcgacgt
     1801 tcatggcgct cgatgccaag accacggcca ctcctctcgt cctcctcaag gaggagggga
     1861 tggcgaaact cctcgagatc tatcacacga aagcggcatt atatcaggac ctgttcatcg
     1921 ggaaccgggc cggctccctc ggcgagacct cggtcatcgc cctgctgctc ggagcggcgt
     1981 tccttatgat gaaacggtac atcacctggc atatcccgat cccgttcatt ctcacagtcg
     2041 gggtagttac ctggctcttc ggcggcaaag agggcctcat gaccggcgat ccgatcttcc
     2101 atatgatgtc
//
LOCUS       DASSLD010000258         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_494065,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000258 DASSLD010000000
VERSION     DASSLD010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494065"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..309
                     /locus_tag="VFK23_04675"
                     /pseudo
     CDS             70..309
                     /locus_tag="VFK23_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            312..1322
                     /locus_tag="VFK23_04680"
     CDS             312..1322
                     /locus_tag="VFK23_04680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7318414.1"
                     /translation="MKQRISIIIPNYNGGRTIGICLDSVFAISDEEREVIVVDDCSRD
                     GSLDIVRKYPCRVVQMERHAGASAARNAGAFNSSGDILFFIDADCILQRDALSIIRRC
                     FQEYASGTVFGGTYSWAPYDPGFFNAFQSGFIHYFETKNAGAPDYLATHALAVSAETF
                     RKVGGFTEGFLPILEDGEFSHRLRRSGHRLVMIPGLQVRHIFNFSLGRSLKNAVRKTR
                     YWIEYSLPHKDLFADSGTASKELKINGTVWLANAVLALLFLLTGKPWLLAPILALWSA
                     GIFVNRHLFGAFFRAGGAGFAVMAALYYSVVYPSAVWLGAFGGFVQHFRGARSGVQSE
                     QA"
     gene            1341..>2370
                     /locus_tag="VFK23_04685"
     CDS             1341..>2370
                     /locus_tag="VFK23_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7318415.1"
                     /translation="MYSPFKYIRDIFWKGRPIHLTFFITRRCNSRCPFCFYLKADDVP
                     SGTPPELSLSEIEKISSSLGSLLWLAFSGGEIFLRDDLVEIAETFYRNNTPAIMLFPT
                     NGLLPEVIRERMETIVRSCPRSVVALKLSLDGLGEAHDALRKTPGSFAKTIQTYEAVS
                     GMVERYPNFELGINTVFCSENQDAMDEIIDYVGGLKYIRTHTISLVRGNLSDKHYAGA
                     DINKYHEAVLKLEKNLREGRSAVYRFRGGRIKAAQDILQRRLIDRTAREHRRTIPCYA
                     GRLNLVLSEYGEVFPCELLQDSFGNVRDHGYDILKVCDSVKARGVIASIADGRCHCTH
                     ECYFMTNIL"
BASE COUNT          466 a          671 c          690 g          543 t
ORIGIN      
        1 gaggtggtcc tgatcctggc ctcattgttt ccttctgcgg ccggcaggga gattgacttc
       61 tcggcccagt acagcccgag gcccgaatgg tatttccttc cgctctatca actcacgaaa
      121 tatttcccgg gcaggtggac gttcgtgggc gccgtggccc tcccggggct ggccttcggt
      181 cttctgctcc tggcgccctt tctggacggc ggcagagaga ccgcgctgcg ccagcggaag
      241 accgccgcgg ccgtggggtt cggacttttg gttgcagtga tggtcctgac gattgcatcg
      301 ctgctctaac gatgaaacag cgtatatcca tcatcattcc caattacaac ggcggccgga
      361 caatcggaat atgccttgat tccgtctttg cgatcagcga cgaggaacgg gaggtcatcg
      421 tcgttgacga ctgttccagg gacggttcgc tggacattgt caggaagtat ccctgccggg
      481 tcgttcagat ggagaggcat gccggcgctt cggcggcgcg gaatgccgga gcgttcaaca
      541 gctcgggcga tattctgttt tttatcgatg ctgattgtat cctgcagcga gacgccttgt
      601 cgatcatccg gagatgtttt caggaatacg cgtcgggaac ggtcttcggc ggcacctatt
      661 cttgggcccc ctacgatccg ggattcttca atgccttcca atccggtttc attcattact
      721 tcgagaccaa gaacgccgga gcgcccgact acctcgcgac gcacgccctc gccgtcagcg
      781 cggaaacctt caggaaggtc ggcggcttca ctgagggttt tctgccgatc ctcgaggacg
      841 gtgagttcag tcatcgcctg aggagaagcg gccacagact cgtcatgatc cccggccttc
      901 aggtccggca tattttcaat ttttcgctcg ggcggtctct gaaaaacgct gtcaggaaga
      961 cccgctactg gatcgagtat tcattgccac acaaagacct gtttgccgat tccggaacgg
     1021 cgtcgaagga gctcaagata aacgggacgg tctggctggc gaacgccgtc ctggcgctgc
     1081 tttttctgct gaccggaaag ccgtggcttc tggcgcccat cctcgcgctg tggagtgccg
     1141 gaatcttcgt gaacaggcac ttgttcgggg cgttcttccg ggcgggcgga gccggctttg
     1201 ccgtcatggc ggccttgtat tattcggttg tgtatccgtc ggcggtgtgg cttggggcgt
     1261 ttggcgggtt tgtgcagcac tttcgggggg cccgttccgg agtgcagtct gaacaggcat
     1321 gagttaaatt ggtgcaacga atgtactcgc cgttcaaata cataagagac atattctgga
     1381 aaggaagacc gatccatctt acgttcttca tcaccagacg gtgcaattca cgatgcccct
     1441 tctgcttcta cctgaaggct gatgatgtcc ccagcggtac gccacctgag ttgtcgctat
     1501 ccgagatcga aaaaatatcg tcttcgctgg gcagtctcct gtggctcgcc ttttccggag
     1561 gggagatctt tctgagggac gatcttgttg agattgccga gaccttttac cggaacaata
     1621 caccggctat catgctgttc cccacgaacg gactccttcc cgaggtgatc cgcgagagga
     1681 tggagacaat cgtgcgctcc tgtccccgga gcgtcgttgc gctcaaactg tccctcgacg
     1741 gtctgggaga ggcccatgac gcccttcgca agacaccggg aagttttgcg aagaccattc
     1801 agacatacga ggccgtcagc ggcatggtcg aacgttaccc gaatttcgaa ctcggaatca
     1861 atacggtctt ctgctcggaa aatcaggacg ccatggatga gatcatcgac tacgtggggg
     1921 gattgaaata catcaggacg cacaccatct ctcttgtccg cggcaacctt tccgacaaac
     1981 actatgccgg tgcggacatc aacaaatatc atgaggccgt cctaaagctt gaaaagaacc
     2041 tgcgggaagg aaggtcagcc gtgtatcgct tcaggggcgg ccgcatcaag gcggcccagg
     2101 atatcctcca gcgccggctc atcgatcgaa cggcgcgcga gcatcggagg acgattccct
     2161 gctatgcggg caggctcaat ctcgtgctct ccgaatacgg ggaggtcttc ccctgtgaac
     2221 tgcttcaaga ctcgttcggc aatgtccggg accacggata cgacatcctg aaggtctgcg
     2281 actcggtgaa agcccgcggc gtgattgcct cgatcgcgga cggcaggtgc cactgcaccc
     2341 acgaatgcta tttcatgacc aatatcctgt
//
LOCUS       DASSLD010000259         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1474936, whole genome shotgun sequence.
ACCESSION   DASSLD010000259 DASSLD010000000
VERSION     DASSLD010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474936"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFK23_04690"
     CDS             <1..465
                     /locus_tag="VFK23_04690"
                     /inference="COORDINATES: protein motif:HMM:NF036850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET7318416.1"
                     /translation="RAVGFCLALAKAERIVFHDVDAGKAARLARDIRKKTGVISDAIS
                     SHGLSAAARDAHCLINATPLGLKKSDPLPLGRELLLPHHLVCDLVYNPPETGLLRAAK
                     DRGARRLPGIGMLLFQGVIAFEIWTGAQAPVAVMKNALARQIAARARETGSR"
     gene            745..2586
                     /gene="pilB"
                     /locus_tag="VFK23_04695"
     CDS             745..2586
                     /gene="pilB"
                     /locus_tag="VFK23_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HET7318417.1"
                     /translation="MSGRLGSMLVSSGLITDEQLKKALSVQKTEGGRLGSILVKLGFV
                     QEDKLMTFLSKQYGVPYVDLSKFEINPAVIKHIPAEVAQKYRIMPINRAGATITIAMV
                     DPSNIFAIDDIKFMTGYNVEAVVATEGAIVEAVKRYYGGARNLVSAKPAGGAASASQK
                     EEKKLTIEAKDYALDEMQMEDSAIDDIDVGGVVDVNDFDDLVHGAVDDLEVVEDQSLD
                     QLSGEVEAPIVKLVNGILLRAAKLGVSDIHVEPYEKKFRVRYRLDGVLSTVMGLPLKI
                     RNAIISRIKIMASLDIAERRLPQDGRIKLKMGRNKVMDYRVSVLPTLFGEKIVLRLLD
                     KSNLQLDMTKLGFDPDQLTDFKEALGRPFGMVLVTGPTGSGKTTTLYSALSELNKDSD
                     NLMTAEDPVEFNLMGINQVQMKDEIGLNFAAALRSFLRQDPDIIMVGETRDYETAEIG
                     VKAALTGHLVLTTLHTNDAPSTINRMLNMGVEPFLVSSAVILIVAQRLVRKACANCKK
                     PEKLPVQTFIDAGFTPEEAKDVVSYKGTGCDVCNRTGYKGRVALYEVMPIREEIKELI
                     LQGASVFDIKKQAIAGGMKTLRRSGLLKVKTGLTSLEEVVENTVPDS"
BASE COUNT          620 a          757 c          763 g          484 t
ORIGIN      
        1 cgggccgtgg gcttctgcct cgcgctcgcg aaggcggaaa ggattgtctt ccacgacgtc
       61 gacgccggga aagccgcaag gctcgcacgt gatatccgga aaaagaccgg cgtcatttcc
      121 gatgcgatat cttctcacgg cctttccgcc gccgcaagag atgcccactg cctgatcaac
      181 gccacgccgc tcggactcaa aaagagcgat ccgctcccgc tcggcaggga actgctcctg
      241 ccgcatcacc tggtatgcga tcttgtctat aatccgcctg agaccggatt gctcagggcg
      301 gcgaaagacc ggggcgcgcg gcgcctcccc ggcatcggca tgcttctgtt ccagggagtc
      361 attgcgttcg aaatctggac cggggctcag gcccctgttg cggtcatgaa aaacgcactt
      421 gcccgccaga ttgctgcacg cgcgcgggag acgggttcga ggtgaggtca catcaaagtc
      481 gactgcaagc tcaccatttc atcattccca agcgaagcac gtcgagagtc tcgtgaataa
      541 tttagagacg ccccccccag caggcgggac gggttcataa gccggaatga caccaggaaa
      601 gatcgaatat gggaagaaat gcttttgtga tcaggaacac aaccctcgga atgagcagtc
      661 ttatcttgac agggaatgag tattaaagta atataatcac tgattttttt cagttgaagg
      721 agttttgaag gagtttctat ggcgatgtcc ggaaggctcg gctctatgct tgtgtcgtcg
      781 ggcctgataa ccgatgagca gctcaagaag gcgctctcgg tgcaaaagac ggagggcggc
      841 aggctgggtt ccattctggt caaactgggg tttgtccagg aagacaagct gatgaccttc
      901 ctgagcaagc agtacggagt gccttacgtc gacctcagca aattcgagat caaccccgcc
      961 gtcataaagc atatacccgc tgaagtggcc cagaaatacc gaatcatgcc gatcaaccgg
     1021 gccggcgcca ccatcactat agccatggtg gacccctcga acattttcgc catcgatgac
     1081 atcaagttca tgaccggtta caacgttgaa gcggtggtgg cgaccgaggg ggcgatcgtc
     1141 gaagcggtta aaagatatta cggcggtgcg aggaacctcg tttccgccaa accggcgggg
     1201 ggcgccgcgt ctgcctctca aaaagaagaa aagaagctga ccatcgaagc caaggactat
     1261 gccctggacg aaatgcagat ggaagattcg gcaatcgatg acatcgacgt cggcggcgtc
     1321 gtcgatgtca acgatttcga cgaccttgtc cacggcgcag tcgatgacct cgaggtcgtg
     1381 gaagaccaga gcctcgacca gctttccgga gaagttgaag cgcccattgt caaactcgtg
     1441 aacggcatcc tcctgcgcgc ggcaaagctg ggcgtctcgg acatccatgt cgaaccctat
     1501 gaaaagaagt tccgcgtaag gtaccgcctg gacggcgtcc tgagcaccgt gatgggactt
     1561 cccctcaaga tcaggaacgc catcatttcc cggatcaaga tcatggcgtc cctcgacatc
     1621 gccgagcgcc ggcttcccca ggacggcagg atcaagctca agatgggaag gaacaaggtc
     1681 atggattacc gggtttcagt gctcccgacc ctcttcggcg aaaagatcgt gcttcgcctg
     1741 ctcgacaagt cgaacctcca gctcgacatg acaaaactgg gtttcgatcc ggaccagttg
     1801 accgatttca aggaggcgct cggcaggccc ttcggcatgg tcctcgtgac cggaccgaca
     1861 ggaagcggca agacgaccac cctctattcg gcactctccg agctcaacaa ggactccgat
     1921 aacctcatga ccgccgagga cccggtggag ttcaacctca tgggcatcaa ccaggtgcag
     1981 atgaaggacg agatcgggct caacttcgcc gccgcgctcc gttcgttcct ccggcaggac
     2041 ccggacatca tcatggtggg agagacccgc gactacgaaa ctgccgagat cggcgtcaag
     2101 gcggcgctta ccgggcacct cgtgctcact acgctccata cgaacgacgc cccgagcacc
     2161 atcaaccgca tgctcaatat gggcgtcgaa cccttcctcg tgtcgtcggc ggtcatcctc
     2221 atcgtggcgc agcggctcgt gcgcaaggcc tgcgccaact gcaaaaagcc ggagaaactt
     2281 ccggtgcaaa cctttatcga cgcgggtttc acgcccgagg aagcgaagga cgtcgtcagt
     2341 tacaagggca cgggctgcga tgtctgcaac aggaccggct acaagggcag ggtcgcgctc
     2401 tacgaggtta tgccgatcag ggaagagatc aaggaactga tcctgcaagg ggcgtccgtc
     2461 ttcgacatca agaaacaagc catagcgggg ggcatgaaaa ccctgcgaag aagcggcctc
     2521 ttgaaggtga agacggggct tacctccctc gaagaagtgg tggagaatac ggtccccgat
     2581 tcatgaggga aacgcggcgt gcggcgaaac cgaacgacgt tccg
//
LOCUS       DASSLD010000260         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1083143, whole genome shotgun sequence.
ACCESSION   DASSLD010000260 DASSLD010000000
VERSION     DASSLD010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083143"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..455
                     /locus_tag="VFK23_04700"
     CDS             306..455
                     /locus_tag="VFK23_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HET7318418.1"
                     /translation="MLWTIAVILLVLWAVGLITAYTLGGFIHILLGLAVVVILINIIQ
                     GRKAV"
     gene            926..1342
                     /locus_tag="VFK23_04705"
     CDS             926..1342
                     /locus_tag="VFK23_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318419.1"
                     /translation="MITHLVVFLIGALSGASGHYLGTKYTEQRHKKESDIIASKKLEH
                     LKNIMPKLIKEMSEDIKSDKTKIVREFVVLQNKRTGIGGMGQPRFAYYEDEHDNLKGE
                     IDILEEKSYVVDVTPGNIPMYRMTEEFVNLLLKYGK"
     gene            complement(1499..>2109)
                     /locus_tag="VFK23_04710"
     CDS             complement(1499..>2109)
                     /locus_tag="VFK23_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7318420.1"
                     /translation="ARIETERLKKEAGKALTEAEQARAAAAVEAAQAAQAKLEAEQAR
                     LAAQAEAEKAVKAKADADQLMKELSELKAQQTERGIVLTIGDVLFATGKANLSPAANK
                     SVAKLAEFLRKYPTRNVLIEGHTDSVGSDDFNLALSQQRANSVKARLVADAVDPARIT
                     TVGYGKKYPVASNDTKAGKAQNRRVDLIILNEGVKAETQMRQ"
BASE COUNT          517 a          530 c          584 g          478 t
ORIGIN      
        1 gcgctgagct actccgaaag agtggatcga agagcgagtt tttcggatca aggaggcacg
       61 ggtttcccca ccacaatgcc ccaatcctgg ccccgcgcgt cttccgccga gtaccaatca
      121 aggcatagga caaacaccgg accgagaatc agacagacca cgattcgcgt ttcggacaga
      181 agccgatagc gaaggctgga gggcaggatt accgtgtgaa tatacgataa tgcgtgcgct
      241 tgccgaatga aggacaaggg gaaagcaggc atcgatgttg ggcgataggg gggaggtgac
      301 gaaaaatgct ctggaccatc gcggtcattt tgcttgtact ttgggcagta ggattgatca
      361 ccgcgtacac gcttggcgga ttcattcaca ttctcctggg cctcgcggtc gtggtgatac
      421 tgatcaatat cattcaaggt cgaaaggccg tgtagcgaac atggattgca actctgagga
      481 gaggaggtca agcgatggta gccagaaagt tgttcatgtt ttatgtagtt gtaatgattc
      541 ccatgggtct cttaagcctt agccttgccg cgggaatccg tagcccgcga gaagaagggg
      601 gagaataaat tcatctaaca gcacttggga ccccggagga cgcgatgatt tgcccgaact
      661 gtaaacgcga tgccaaaacc gtgaagaccc aaggggtcct tcgatatagg aacatgtatg
      721 gaaaagagat cgctgttcca tggtggggag aatattgtaa ggagtgtggg cataagttcc
      781 acactctcga acaggaagaa atgcacagtc gtgagaaaga gaaaatggtg aaagaatccg
      841 aggagtcaaa ataatacaat ccttcctcca ccggcagggc gtccgcttgc tatgatggcg
      901 aggatattga tccggaggtc aaatcttgat tacccacttg gtcgttttcc tcatcggagc
      961 cttgtctgga gcctctggtc attatctcgg aactaaatac acggagcagc gacataaaaa
     1021 agaatcggat attattgcga gtaaaaagct tgagcatcta aaaaatatta tgccgaaatt
     1081 aataaaagaa atgtcggaag acataaagag tgataagaca aagattgtaa gagagtttgt
     1141 tgttcttcaa aacaaaagga caggaatagg tggcatgggc cagccaaggt ttgcatatta
     1201 tgaagacgaa cacgacaacc ttaaggggga aattgatatt ttagaagaaa aatcctatgt
     1261 cgtagatgta acacccggaa atattccaat gtatcgtatg accgaagaat ttgtgaatct
     1321 attattgaaa tatgggaaat agaaaaccga agatcgtccg attgcgatga tggtgaggct
     1381 cttattggaa gaggcgatgt ggagtcggga cgcatcgggg catattgggg aagggcgggg
     1441 aaacctcccc gcccttccat tggagaatca tccgaagatg cgaccttcta cgcgtcggtt
     1501 actgccgcat ctgggtctcg gccttgaccc cttcgttcag gatgatcaag tccacgcgac
     1561 ggttctgcgc cttgccggcc ttggtgtcgt tgctggccac tgggtatttc ttcccgtacc
     1621 ccacggtggt gatccgtgcg gggtccaccg cgtctgcaac cagcctcgct ttcaccgagt
     1681 tcgccctctg ctgggagagg gccaggttga aatcatccga cccgacgctg tccgtgtggc
     1741 cctcgatcag cacgttcctg gtcggatact tccttaaaaa ttcggccagt ttggccacgc
     1801 tcttgttcgc ggcaggggaa agattcgctt ttccggtggc aaacagcacg tccccgatcg
     1861 tcaggacgat cccccgctcc gtctgctggg ccttcaactc cgagagctcc ttcatgagct
     1921 gatcggcatc ggccttggcc ttgaccgcct tctccgcttc cgcctgggcg gccaaccggg
     1981 cctgctccgc ctccagcttc gcctgcgcgg cctgggccgc ctcgaccgcc gccgccgccc
     2041 tggcctgttc cgcctcggtc agcgccttcc ccgcttcctt cttcaaccgt tccgtctcga
     2101 tcctggcac
//
LOCUS       DASSLD010000261         3366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_93619,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000261 DASSLD010000000
VERSION     DASSLD010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93619"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VFK23_04715"
     CDS             <1..567
                     /locus_tag="VFK23_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HET7318421.1"
                     /translation="DTTLDYESLGKVGSIMGSGGMVVLDETDCMVNVAKYFLQFTQSE
                     SCGKCVPCRVGTKRLLEILTRITQGKGREGDIDLLQTLSSDVKTSSLCGLGQSAPNPV
                     LSTIKYFRDEYEAHIYEHRCPAGVCKDLLTYSILEEFCKGCTACTRVCPAGAIMGEKK
                     KPHKIVQDLCIKCGACFETCKFKAIKKG"
     gene            573..3245
                     /gene="fdhF"
                     /locus_tag="VFK23_04720"
     CDS             573..3245
                     /gene="fdhF"
                     /locus_tag="VFK23_04720"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1608..1610,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HET7318422.1"
                     /translation="MKLTINNISITAPEGVTILDAARRNDIYIPTLCDDPRLEPHGGC
                     RLCLVQVKGMPRPVTACTTPATEGMEVETSNEPIERQRRTIVELLLSDHPNDCMVCEK
                     AGDCTLQELAYFYDLRSNRFWGERRRYTKKDMNPFIERDMEKCILCGKCVRVCDEVQG
                     LGAIDISGRGFTAKVTPAFEKDLDCEFCGQCVSICPTGALMSKQALGKGRQKDVRQVE
                     TVCSYCGCGCNLTLHVSRNEVVRVTSRRDTINEGWLCVKGRFGQGFINSPDRLTTPLI
                     KKDGAFVPATWDEALGYIAERLSAIKKKHGPDAIGGLSSARCTNEENYLFQKFMRAAI
                     GTNNVDHCARYUHSTTVAGLATVFGSGAMTNSIAEIENNDVLFIIGSNTKENHPIVAL
                     RMIKAKRKGAKLIIADPRRVPLVRFADIWMQHKPGTDVALLNGMMHVILKEGLADKDF
                     IASMTEGFDEAFRKNLDEFTPEAASKITGVPAETIMKAARLYAGSAKAGIYYTMGITQ
                     HAHGTENVFSIANLALLTGNLGKESAGVNPLRGQNNVQGSTDMGCIPNNYPGYQRVSI
                     PAIQQKFEELWKAKLSANEGLTAPEMLDAAEKGSLKALYVMGENPVVSDPNVAHTVHA
                     LKALDLLVVQDIFMTETAELAHVVLPGTVFAEKDGTFTNTERRVQRVRHAVNPPGQAM
                     KDSQIIMELSKRMGYEMHYPHTADIFREIRRAWSALEGMSYARLQDGGLQWPCPTTDH
                     PGTPYLFKGGFPRGKGRFTLVTYKPSIEQSDKEYPFILTTGRQLFQYHTGGMTRRVKP
                     IDTVSPESYVEINPADAQALAIEGGAKVKVSSRRGSITVKAVVSKRPAQGVVFIPFHF
                     KEAAANVLTSSTSLDPIAKIPSFKVSAVRIEKAL"
BASE COUNT          730 a         1050 c         1018 g          568 t
ORIGIN      
        1 gacaccacgc tggactatga atcgctcggc aaggtcggct ccatcatggg ctcgggcggc
       61 atggtggtcc tggacgagac cgactgcatg gtaaacgtgg ccaagtactt cctgcagttc
      121 acgcagtcgg aatcctgcgg aaaatgcgtg ccgtgcaggg tcggcaccaa gcgacttctc
      181 gagatactta cccggatcac ccaaggaaag ggcagggagg gggacatcga ccttctccag
      241 accctctcca gcgatgtgaa gacctcgtcg ctttgcggac tcggccagag cgcgccaaac
      301 ccggtcctct ccacgatcaa gtatttccgc gacgagtacg aggcgcacat ctacgaacac
      361 cgctgccccg caggcgtctg caaggatttg ctcacgtatt ccattcttga ggaattctgc
      421 aagggctgca ccgcctgcac gcgcgtctgt cccgccggcg ccatcatggg cgagaaaaag
      481 aaaccacata agatcgtgca ggacctgtgc atcaagtgcg gcgcctgttt cgagacctgc
      541 aagttcaagg cgatcaaaaa agggtaacga taatgaaact gacgatcaat aacatttcca
      601 taacggcccc cgagggcgtc accatccttg acgccgcccg gcgaaatgac atctacatcc
      661 ccacgctctg cgatgatccc cggctcgagc ctcacggcgg ctgcaggctc tgcctggtcc
      721 aggtgaaggg catgccccgg ccggtgacgg cgtgcaccac gccggcgacg gaaggcatgg
      781 aggtggagac ctcgaacgag ccgatcgaac gccagcgccg gaccatcgtc gaactcctcc
      841 tctccgacca tccgaacgac tgcatggtct gcgagaaggc cggcgactgt acgctccagg
      901 aacttgccta cttctacgat ctccgctcca accgcttctg gggcgagcgg cgccggtaca
      961 ccaagaagga catgaacccc ttcatcgagc gcgacatgga gaagtgcatc ctgtgcggca
     1021 agtgcgtccg ggtctgcgac gaggtccagg gcctgggagc gatcgacata tcaggcaggg
     1081 gtttcaccgc caaggtgacg ccggccttcg agaaggattt ggactgcgag ttctgcggac
     1141 agtgcgtcag catctgtccc accggtgcgc tcatgagcaa gcaggcgctg ggcaagggca
     1201 ggcagaagga cgtgaggcag gtcgagaccg tgtgctccta ctgcgggtgc gggtgcaacc
     1261 tgactctgca cgtctcccgg aatgaagtgg tgcgcgtcac ctcgcgccgg gacacgatca
     1321 acgaaggctg gctctgcgtc aaggggaggt tcggccaggg tttcatcaac agcccggacc
     1381 gtctcacgac gccgctcatc aagaaggacg gcgcgttcgt ccccgccacc tgggacgagg
     1441 cgctggggta catagccgaa aggctctccg ccatcaagaa gaagcacggg cctgacgcca
     1501 tcgggggact ctcctcggca cgctgcacga acgaggaaaa ctacctgttc cagaaattca
     1561 tgcgcgcggc gatcggcacg aacaacgtcg accactgcgc ccgctactga cacagcacga
     1621 ctgtggccgg tctggccacc gtattcggat ccggcgccat gacgaattcg atcgccgaga
     1681 tcgagaacaa cgacgtgctg ttcatcatcg gctcgaacac caaggagaac cacccgatcg
     1741 tcgccctccg gatgatcaag gcgaagagga aaggggcgaa gctcatcatc gccgatcccc
     1801 ggcgggtgcc gctcgtccgt ttcgccgaca tctggatgca gcacaagccc ggcacggatg
     1861 tcgcccttct gaacggcatg atgcacgtga tcctgaagga aggcctcgcc gacaaggact
     1921 tcatcgcgtc catgaccgag ggcttcgacg aggcattcag aaagaacctt gacgaattca
     1981 cgcccgaggc cgcctcgaaa atcaccggcg ttcccgcgga aacgatcatg aaggccgcgc
     2041 gcctctacgc cgggtccgcc aaagccggga tctactacac tatggggatt acgcagcatg
     2101 cccacggcac ggagaacgtg ttctccatcg ccaaccttgc gctcctgacc gggaacctcg
     2161 gcaaggagtc ggcgggcgtc aatcccctgc gcggccagaa caatgtccag ggctcgacgg
     2221 atatgggctg cattcccaac aactatccgg gataccagcg cgtgagcatc ccggcaatcc
     2281 agcagaaatt cgaggaactc tggaaggcga agctctccgc caacgagggg cttaccgcgc
     2341 cggagatgct cgatgcggcc gagaagggct cgctcaaggc gctgtatgtc atgggcgaga
     2401 accccgtggt ttccgacccc aacgtggcgc ataccgtgca tgcgctcaag gcgctcgact
     2461 tgctggtcgt ccaggatatc ttcatgaccg aaacggccga gctcgcccat gtggtcctgc
     2521 ccggcacggt gttcgcagaa aaagacggca cattcacgaa cacggagcgc cgcgtgcaac
     2581 gtgtccgcca cgccgtgaac ccgcctgggc aggccatgaa ggattcgcag atcatcatgg
     2641 agctttcgaa gcggatgggc tacgagatgc actatccgca tacggccgac atattccgcg
     2701 agatacgccg ggcatggtcc gcccttgagg gcatgagcta cgccagactg caggacggag
     2761 ggcttcagtg gccctgcccc acgaccgacc atccgggtac gccgtacctg ttcaagggag
     2821 ggttcccgcg cggcaagggc aggttcacgc tggttaccta caaaccgtca atcgaacagt
     2881 cggacaagga atatcctttt atcctgacca cggggcggca gctgttccag tatcacaccg
     2941 gcggcatgac ccgccgcgtg aagccgatcg acaccgtgtc gccggagtcg tatgtcgaga
     3001 tcaatcccgc cgacgcgcag gccctcgcga tcgagggcgg cgcgaaggtg aaggtcagtt
     3061 cgcggagggg ttccatcacg gtgaaggcgg tggtttccaa gcggcccgcg cagggcgttg
     3121 tgtttatccc gttccacttc aaggaagcgg ccgccaatgt cctgacgagc agcacctcgc
     3181 tcgatcccat agccaagatc ccgtcgttca aggtctccgc agtgcggatc gaaaaagcgt
     3241 tatagcaggc ccgcccggga ggagcagagc gccgggtacc cgatgattca atttcatggc
     3301 taggattcta tctccaaggt gaattttaac gattttttcc agcgaggggg acacgatggc
     3361 cgacaa
//
LOCUS       DASSLD010000262         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1242441, whole genome shotgun sequence.
ACCESSION   DASSLD010000262 DASSLD010000000
VERSION     DASSLD010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242441"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /locus_tag="VFK23_04725"
     CDS             complement(<1..1652)
                     /locus_tag="VFK23_04725"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7318423.1"
                     /translation="MEAASRACVTGGRIPLMPGFRSLQGRILTFFLGLFILVYVLAFL
                     AVNTAVIRNAHAQIKNELLAGGRVFDRLIKVRTDRLVLAARTLSGDYGFKTTFAAGDK
                     PTLLSAMRNHLGRIQADVMQLVSMDGNITADTLHPAATPEKFYLPGLIETAEQSGEAS
                     AIVFIEGRLYQVVVVPLLAPVPVAWICMGFTIDDTLARELQSLHTVDVTLVRERPREL
                     PVIFGSTLSGDLRQVFAAAVYSAAWDPEGTAITDMRGVKYVSLSLELGKQGDSSVKVF
                     LQRSLEQALLPFNRLRKILLVLFVAGIGIILAVGVLIAGSVTKPVRLLAESARSIEEG
                     DYTHTVPPGRQDELGRLSSAFNQMIGAIAQREERIKFQAYHDTLTGLPNRAYLHQHLE
                     KAVAAARAEKAPLALIAMDISRLRDVNAALGHATGDVILQKVGSLLGSSSRDFAVVAR
                     IGGDEFAVLLRAGQGIEGAVLYVQKALQQLETPVLVSETPIQIEASFGVAAYPEHGDD
                     ADALLRHAETAMHLAKATTRGYFVYVPESEERRLRQLTLLGEL"
     gene            complement(1604..>2354)
                     /locus_tag="VFK23_04730"
     CDS             complement(1604..>2354)
                     /locus_tag="VFK23_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318424.1"
                     /translation="PTRSIRQASSTLTDVRLLRKERMISSLRHRWVLLAFLAAGLTAF
                     ATGASAMNVRVTVQDDAGNPVKDAVVYATNLDKNAPLPHPVREAVMDQQDEEFVPYVL
                     PVQAGTTVYFPNKDNIRHHVYSLFRPKKFELMLYKNSPPAPVLFDQPGVVVLGCNIHD
                     WMIAYILVLETPWFAKTGDDGKAELRNLPNGAYEIRAWHPLMKETTESTAKNVTAKEG
                     TGGDVDLEFRITRKPEWRPHRVPASPGGGYR"
BASE COUNT          475 a          706 c          713 g          460 t
ORIGIN      
        1 agctccccca gcagggtcaa ttgacggagc cgccgttcct ccgactcggg gacatacacg
       61 aaataaccac gcgtcgtggc cttcgcaaga tgcatcgccg tctccgcatg tctcaagagc
      121 gcatcggcat catccccgtg ctcgggatag gccgccaccc cgaagctcgc ttcgatctgg
      181 atgggggtct cgctgaccag gaccggcgtt tcgagctgct gcagtgcttt ctgcacatag
      241 agcacggcgc cttcgatgcc ctgccccgcg cgcaggagca ccgcaaactc gtctccgcct
      301 atgcgcgcca caacggcgaa atcccgggat gaactaccga gaagggaccc cactttctgc
      361 aggataacat cgcccgtcgc atggccgagc gcggcattca catccctaag gcggctgatg
      421 tccatcgcaa tgagtgcaag aggggccttt tccgcgcggg cggcggcgac cgccttttcg
      481 aggtgctggt gcagatacgc ccggttcggc aggccggtca gggtatcgtg atatgcctgg
      541 aatttgatcc gctcttcccg ctgggcgata gccccgatca tctggttgaa tgccgaagac
      601 agtctgccga gttcatcctg cctgcccggc ggcacggtat gcgtgtaatc gccctcttcg
      661 atgctgcggg cgctttccgc gagcagacga acgggctttg tcactgatcc ggctatgagg
      721 acccctacgg caaggatgat gccgataccg gcgacaaaaa gaacgagcag gattttccgg
      781 aggcgattga acggaaggag ggcctgttcc agggagcgct gcaggaaaac ttttacggac
      841 gaatcaccct gtttgccgag ctccagggaa agacttacat atttgacgcc ccgcatgtcc
      901 gtgatggcgg tcccttcggg atcccatgcc gctgaataga ctgccgcagc gaagacctgc
      961 cgcaggtccc cggatagggt cgaaccgaag atgaccggga gctctcgagg gcgttcccgc
     1021 acgagcgtga catcaactgt atggagggac tgaagttccc gggccagggt atcgtcaatc
     1081 gtgaagccca tgcatatcca cgctacgggc accggcgcca gcaagggaac gacgacaacc
     1141 tgataaaggc ggccttcgat gaatacgatt gccgacgcct ctcccgactg ctccgcggtc
     1201 tcgatcagcc cgggaaggta aaatttctcg ggcgtcgcgg ccggatgcag cgtgtccgcg
     1261 gtgatgttgc cgtccatgga aacaagctgc atgacgtcgg cctgtatgcg gccaaggtgg
     1321 ttgcgcatcg cggaaagcag cgtgggcttg tcgccggcgg cgaaggtggt cttgaatccg
     1381 taatcgcccg ataaagtcct ggctgccaga accaggcgat ccgtacggac cttgatgaga
     1441 cggtcaaaaa ctcttccgcc agccagcagt tcattcttga tctgtgcgtg ggcattccgg
     1501 atgacggcgg tgttgacggc aagaaatgcg agcacataga caaggatgaa caacccgaga
     1561 aagaaggtca ggatgcgtcc ttgaaggctc cggaagcccg gcatcaacgg tatcctcccc
     1621 ccggtgacgc aggcacgcga tgcggcctcc attcgggctt ccgcgtgatc cggaactcga
     1681 ggtcgacatc gcccccggtc ccctccttcg cggtcacgtt ctttgccgtg gattccgtgg
     1741 tctccttcat gagagggtgc caggcgcgga tctcgtaagc gccgttcggg agatttcgca
     1801 gttccgcctt tccgtcatcg ccggttttgg cgaaccacgg ggtctccaac acgaggatgt
     1861 aggcgatcat ccagtcgtgg atgttgcagc cgagcacgac gacccccggc tgatcgaaca
     1921 gaacaggcgc gggaggcgag ttcttgtaga gcatcagctc gaattttttc ggtcgaaaga
     1981 gcgaatagac atgatgcctg atgttgtcct tgttcgggaa atatacggtc gttcccgcct
     2041 ggacaggcag cacgtaggga acgaactctt catcctgctg gtccatgacc gcctcacgga
     2101 ccgggtgcgg cagcggcgca ttcttatcca ggttcgtcgc atatacgact gcgtccttca
     2161 ccggatttcc ggcatcgtcc tgcacggtca cacgcacgtt catcgcggag gcgccggtgg
     2221 cgaatgccgt caaaccggcg gccaaaaacg ccaggagaac ccatcgatgg cgcagtgatg
     2281 aaatcattct ttcctttcgc agcagccgca catcagtaag tgtagacgat gcctgcctta
     2341 tagagcgtgt cggg
//
LOCUS       DASSLD010000263         2271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1259521, whole genome shotgun sequence.
ACCESSION   DASSLD010000263 DASSLD010000000
VERSION     DASSLD010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259521"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VFK23_04735"
     CDS             complement(<1..122)
                     /locus_tag="VFK23_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HET7318425.1"
                     /translation="MDGSSGKRVCVIGAGIAGLVTSKVLKEDGFEVVVFEKQPEP"
     gene            401..1852
                     /locus_tag="VFK23_04740"
     CDS             401..1852
                     /locus_tag="VFK23_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HET7318426.1"
                     /translation="MLKKITSTFILLFWFVMLGLLVERAYLRPSAVVALDAASEEGVR
                     STDEWFGIYQKGAKIGYAHTSVRPEADTYHLYEESELDILALGAVQRVKTVINSYTTR
                     NFLLKYFDFSLQSDATVMKMKGAVVGRKLILDVMTGDQTRTERITLASPPYLSPNIKP
                     AILLAGLETGRKYRFPVFNPATMSTEDADITVESRDRIKVGEAEQNVYRLRESFQGME
                     TTSWVNQVGETLKEESALGYVLLKEPMAEAKKRDKGGPVVDIISLTMIPSDPIKDSSK
                     VTYLRARLKGAPLAGFQLDGDRQALTGDVVEIRGNETPFDYRLPYSSGEMAGYLLPNA
                     LIQSDDRKIIEQAGKILSGEKDARSAARKLNAWVYTVISKKPVVSIPSAIEVLKQREG
                     DCNEHTALYTALARASGIPTRMAAGIVYLEDGFYYHAWPEIWIGAWLAVDPTFNQFPA
                     DATHIRFVTGNLDRQSGIVKLVGKLKVEVLEYR"
     gene            1935..>2271
                     /locus_tag="VFK23_04745"
     CDS             1935..>2271
                     /locus_tag="VFK23_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7318427.1"
                     /translation="MIKLINLTKKYGRLTAVNTVNLEVAAGEVFGFLGPNGAGKTTTI
                     RMMAGLLQPTSGIALIGGYDVQKEPLKAKYITGFIPDRPYLYEKLTATEFMFFVARLY
                     EMNDSRKRIG"
BASE COUNT          537 a          647 c          624 g          463 t
ORIGIN      
        1 ggctccggtt gtttttcgaa cacgacgacc tcgaacccgt cttccttgag cacctttgac
       61 gttacgagtc ccgcgatgcc cgctccgatg acgcacacgc gcttgccgga agatccgtcc
      121 atggatgacg ctccttgagt gatgcttgcc gctgctgata ttccgtctta taacggaact
      181 gtccaggcgg tttttatttg gttccgtcat tcccgaatgc tttaatcggg aatccagtgg
      241 ttttgaaagg cactgggtcc ccgacagaga cattcgggga cgacgaaacg agggcttaag
      301 caataacttg ctatcaatta tagaataagc gaatctggtt tgacaatacc ggctttagcg
      361 gttaacataa cacaaaagca acacactgta tttattcatc atgctcaaga aaatcaccag
      421 tacgttcatc cttctgttct ggttcgtcat gctgggcctc ctcgtcgaac gcgcctatct
      481 gcggccgtcg gcggtcgtcg ccctggatgc ggcatcggag gaaggcgtcc gctcaacgga
      541 cgagtggttc ggcatctacc agaagggcgc aaagatcggc tatgcccaca cgagcgtgag
      601 gcccgaagcc gatacctacc acctgtacga ggaatcggaa ctcgacattc tcgccctggg
      661 cgccgtgcag cgtgtgaaaa cggtcatcaa cagctataca accagaaact tcctgctcaa
      721 gtatttcgat ttttccctgc agtccgatgc gaccgtcatg aagatgaagg gcgcagttgt
      781 cggcagaaag ctcattctgg acgtcatgac cggggaccag acccgcacgg agagaattac
      841 cctcgcctcg ccgccctatc tttcgccgaa cataaagccg gccattcttc tggccggact
      901 cgaaacgggc aggaaatacc gcttccctgt ttttaatccc gcaaccatga gcaccgagga
      961 cgccgatatc acggtggagt ccagggaccg catcaaggtc ggcgaagctg agcagaatgt
     1021 ttaccggctc agggagtcct tccagggaat ggagacaacg tcgtgggtga accaggtcgg
     1081 tgaaacgctc aaggaagaaa gcgccctcgg ctacgtcctt ctgaaggagc ccatggccga
     1141 ggctaaaaag cgggacaagg gagggccggt cgtcgatatc atatcgctca caatgatccc
     1201 ttccgatccg atcaaggact cctcgaaggt tacctacctg cgggccagat tgaaaggcgc
     1261 ccccctcgcg ggtttccaac tggacgggga ccggcaggcg ctcaccgggg acgtcgtcga
     1321 gatccgggga aacgagaccc cctttgacta ccgtttgccc tattccagcg gcgaaatggc
     1381 cggttacctg ctgccgaacg cgctcatcca gagcgatgac aggaagatca tcgaacaggc
     1441 cgggaaaatc ctgtccggtg aaaaagacgc acggagcgcc gcccggaagt tgaatgcctg
     1501 ggtatatacc gtgatcagca aaaagccggt agtgagcatc cccagcgcca ttgaagtcct
     1561 gaaacagcgc gaaggagact gcaacgagca taccgccctg tacaccgccc tcgcccgcgc
     1621 gtcgggcatc ccgacccgca tggctgccgg gatcgtctac ctcgaagacg gtttctatta
     1681 tcatgcctgg cccgagatat ggatcggcgc atggctggca gttgacccca ccttcaacca
     1741 gttccccgcc gacgccaccc acatcaggtt cgtgaccggg aacctcgacc ggcaatcggg
     1801 catcgtcaaa ctggtcggga aactgaaggt ggaggtgctt gagtatcgat agattgtcga
     1861 ttgttgattg ttgatttagt atcacggttt gatcagtgtc aatccgtgtt catccgtggt
     1921 taatattcag gaacatgatc aaactcatca acctcacgaa aaaatacggc aggctcacgg
     1981 cggtgaacac cgtcaacctt gaagtcgcgg cgggcgaggt tttcggattt ctcggtccga
     2041 acggtgcggg aaagacgacg acaatcagga tgatggcggg gctcctccaa cccacgagcg
     2101 gcatcgccct gatcggcggc tacgacgtgc agaaagagcc gctcaaggcc aaatacatca
     2161 ccggcttcat tccggacaga ccctacctgt acgaaaaact cacggcaacg gaattcatgt
     2221 tttttgtggc caggctctat gaaatgaacg attcgaggaa gcgcatcggc g
//
LOCUS       DASSLD010000264         6316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_464447,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000264 DASSLD010000000
VERSION     DASSLD010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464447"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..1988)
                     /gene="hflX"
                     /locus_tag="VFK23_04750"
     CDS             complement(393..1988)
                     /gene="hflX"
                     /locus_tag="VFK23_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET7318428.1"
                     /translation="MPPDIVITPEAARALTEASAQIRRQVGLIIDRSGSIRNVIIGTN
                     KDILIPDLSEHRLGSRRLRGVRFIHTHLKDQPLNQEDLTDLALLRLDMIAALAVRADG
                     LPGDISIAHLVPPNPEEKLYEVLPPLPVHTVGVDAAEFIADIERQMEKAQAAVEVAKK
                     DRAILVSVSKKSRHEQEESIEELKELARSDGITVLDTVVQRLHDINPRYLLGEGKLKE
                     LIIRSQQLGADLIVFDQNLTPAQVKAIGDITEMRVIDRTQLILDIFARRAHSGDGKVQ
                     VELAQLKYRLPRLSERSTALSRLTGGIGGRGPGETRLEVDMRRARDRIRRLEKQLDAL
                     GRARDQRRERRVRSDIPIVSIAGYTNAGKSTLFNALTKSEVKAENLLFATLDTATRRL
                     RFPRDREVVITDTVGFIKDLPKDLLGAFRATLDEMQDADLILHVVDLSNPRFEQQMES
                     VNALLEEIGLDHIPQLIVLNKADLVNPLWAKAIATRFGGVVCSAINPATFGDLRQKIE
                     KRVWKEDESSGFPARETDSVQSS"
     gene            complement(2162..2695)
                     /locus_tag="VFK23_04755"
     CDS             complement(2162..2695)
                     /locus_tag="VFK23_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318429.1"
                     /translation="MPTKKRGKSSSKKAAKRKPAKKALKKKTLAKKSPPRKKAAKKKA
                     LKKKAAIKPAPKKSVKKVIPSAMPAVSAKEITVEPGPPPGPLPPLEEPARNESAVGTV
                     THYYSHLNVAVVQINKGELKTGDTIHVKGHSTDFMQTVESMEYEHRHVDQASAGQSVG
                     IKVKDHAREHDIIYLLG"
     gene            2955..4397
                     /locus_tag="VFK23_04760"
     CDS             2955..4397
                     /locus_tag="VFK23_04760"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HET7318430.1"
                     /translation="MKKRFSAVLFVFFVSAVSIFMASTSVYATNGMNLEGYGPIATGM
                     GGASMAYDNGTAAVMNNPATLGLMPEGNRLDVALGYLGPHIKATCNAGPCAGADASSS
                     ATSFWMPAIGWIQKSGPFAYGIGVFAQGGMGTEYGADSFLALGSGDKVRSELGVGRAI
                     IPFTVDVNKNFTVGVTADFVWATLDLKMALPGTEFLAMAQGTSAFGSASGSLVNSLGG
                     AVQAGQIGVPEWTRFDFSDKSDFTGAAKGTGFAGKIGGVYKVSDAVSVGATYHSKTEL
                     GDLEATGATLSMKASGPATGGQAIVVPITGKIQVKDFQWPQTIGVGVAYQATDKLMIA
                     ADYKWINWADVLKSFKLTFTADANQSGAAGPGGFNLGGQSVDAILFQNWKNQNVFEIG
                     AGYKFTPEFTGRIGFNYANNPIPDQLMNPLFPAIEKSHVTIGAGYMITKASSVDASFT
                     YAPEVKQTNGQQVTVAHSQTNAQVMYSFRF"
     gene            4475..5383
                     /locus_tag="VFK23_04765"
     CDS             4475..5383
                     /locus_tag="VFK23_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318431.1"
                     /translation="MKSLLRKLMLTALFMILLVNAAGAEELLKPFILGSRGPGSVAEK
                     ADVVKAALTGQGFTVAGEYSPYPDAVVIVATNDELKNTAAKSERGGFGAAVRVSVTKV
                     KDEVQVAYTNPVYMANAYRMQGDLVDVAAKLAAALGKKEDFGAKGLTAKKLRSYHYTV
                     GMEYFDEPSVLAEYKSFEEAAAAVEAGLAAGKGGVTKVCRIDIPGKKEAVFGVGMKAA
                     SDADKFMDDKFIMSEIDFKDLKSTAHLPYEMLVSGNKVYALYARFRIAISFPDLSMMG
                     SNSFMHIMDSPKAIRKALTEAAGGKM"
     gene            5623..>6316
                     /locus_tag="VFK23_04770"
     CDS             5623..>6316
                     /locus_tag="VFK23_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET7318432.1"
                     /translation="MKILIIKLSAIGDVAQTLPALEALKKIHPASEITWVVEEAAAGV
                     LEGHPLIDRLLVSRRKAWFRSLMNPLVLARGMGDMVRFVRELRRTRYDIAIDFQGLLK
                     SGIVIGLARADRKIGFDRTRELSYLFLNERLPAYDVERHALERYLDVARYLGAKDPST
                     SCLLPLEKERPVISKRVGEIRAPGRLLVAVNPVARWKTKLWQERKFAELADRLIREKN
                     AVVIFTGSPGDRQ"
BASE COUNT         1359 a         1700 c         1846 g         1411 t
ORIGIN      
        1 aacaaattgt atttaagtta agcaatcaaa atgccacaac ggaaaattcc caggtatcat
       61 tttgatttat aatataactt atgataaatc gaacgttatt ttttttatga cctgaaagtg
      121 tttttgtatg gtatatccta cagacacgtc tcatcataac ctaagatttt aagaaaagcc
      181 catcaaaata tccgattatg gtgtgtatga aatatcatac ccacaggaaa gacaccgtgg
      241 ctcattgtac acaggaccga aatgcaataa tttgcaatat cttcattgag gagcatctgc
      301 agcaaaatca ttgaaatgaa caacaatcag gtgggatttt tttaggtgtg caacacgaaa
      361 atgtttcggt taaacaaggt aaaaaataac tactacgaac tctgaacgga gtcagtctca
      421 cgtgcaggaa accctgaact ttcatcttcc ttccaaaccc tcttctctat tttctgccgc
      481 aaatccccga acgtagccgg gttgatggca gagcagacca cgccgccaaa cctggtcgcg
      541 atggcttttg cccacagagg gttcacgagg tcggccttgt tgagcacgat aagctggggg
      601 atgtgatcaa ggccgatctc ctccaggagg gcgttcaccg actccatctg ctgctcgaaa
      661 cgcggattgc tcaagtccac gacgtgaagg atcaggtccg catcctgcat ctcgtccagg
      721 gtcgcccgga atgcccccag caggtccttc ggcaggtcct tgatgaaccc cacggtgtcg
      781 gtaatgacca cttcgcggtc gcgcgggaag cggagcctgc gcgtggccgt gtcgagcgtg
      841 gcgaagagca ggttttccgc cttgacctcc gacttcgtga gggcgttgaa cagcgtcgac
      901 ttgcccgcgt tcgtatagcc tgcgatcgag acgatgggga tatcgctcct tacacggcgc
      961 tcccgcctct ggtccctggc cctgcccagg gcatcgagct gtttttccag ccttcgtatc
     1021 ctgtcccgcg cgcgccgcat atcgacctcg agccgcgttt ctccggggcc cctgccgccg
     1081 atgcctcccg tgagacgcga gagggcggtc gaacgttcgg aaagtcgggg cagccggtac
     1141 ttgagctgcg cgagttccac ctggaccttg ccgtcgccgc tgtgcgcccg cctcgcgaag
     1201 atgtcgagga tgagctgggt gcggtcgatg acccgcatct cggtgatgtc cccgatggcc
     1261 tttacctgcg ccggcgtcag gttctggtcg aagacgatga ggtccgcccc caattgctgg
     1321 gagcggatga tgagctcctt caattttccc tcgcccagaa ggtatctggg attgatgtcg
     1381 tgcagccgct ggaccaccgt gtccaggacc gtgatgccgt cggagcgggc gagttccttg
     1441 agctcctcta tcgattcctc ctgttcgtgc ctgcttttct tcgacacgga aacgagtatg
     1501 gcccggtcct tcttcgcgac ttcaaccgct gcctgggcct tctccatctg gcgttcgatg
     1561 tcggcgatga attcggctgc atcgaccccg acggtatgga cggggagcgg cggcaggacc
     1621 tcatacagtt tctcttcggg attcggcggg acgagatggg cgatcgagat gtccccgggc
     1681 agcccgtcgg cccgcaccgc aagcgcggcg atcatatcca gccgcaagag ggcaaggtcc
     1741 gtgaggtctt cctggttcag aggctggtcc ttgagatggg tatggatgaa gcggactccg
     1801 cgaagacgcc tgcttcccaa acgatgctcg gaaagatcgg gaatgagaat gtctttgttt
     1861 gttccgatga tcacgttccg aatgctgccg gaacggtcga tgatgaggcc gacctgcctc
     1921 ctgatctgcg ccgaggcctc ggtgagggcg cgggcggctt cgggggtgat gacgatgtcc
     1981 ggaggcatct ttctccggta cagccgctcg agcagtctga tttcagcagg cttgaggccc
     2041 gttatgttgc catggatagc ggagatggat ggctcctttc ataaattccg gtagaaacag
     2101 atacagcgca gggactctct cctgcgctga tctcattgta tcatacgtga agcaatgcct
     2161 gttaccctaa cagatagatg atatcgtgtt cccgcgcatg gtcttttacc ttgatgccga
     2221 cgctctgacc ggcagatgcc tggtcgacgt gtcggtgctc atactccatg gactcgacgg
     2281 tctgcatgaa gtccgtggag tggcctttga cgtggatggt gtccccggtc ttgagttccc
     2341 ctttgttgat ctggactacg gcgacgttca ggtggctgta atagtgcgtt acggtgccga
     2401 ccgcggattc gttcctggcg ggctcttcca gcggaggaag cgggcctggg ggagggcccg
     2461 gctccaccgt gatttctttc gcggataccg ccggcatggc cgaggggatt acttttttta
     2521 cggacttttt gggtgccggc tttatcgcgg ctttcttctt gagcgctttc ttttttgcgg
     2581 cttttttccg gggaggagac tttttcgcga gggtcttctt tttcagggcc tttttggcag
     2641 gttttcgctt agcggctttt ttcgatgaac ttttgcccct tttttttgta ggcatcagga
     2701 acctcctcct cttttatgat gtcatgccgg actcgatcgg gccgcgccag actttgtcgg
     2761 agaatcgttt ttttcggccg agatggcaaa attgtaacaa aaacacgcta aaaagcaagc
     2821 caaaaaaagc atgaccgcct ctatatgaat gcttgacaga aagcggcaaa tgtgaaataa
     2881 tcgcgcgaaa ttgttatcga gtatcgaaac aacgaaatgg ggtggcaata aagccagagg
     2941 ggaggaggaa aagtatgaag aaacgttttt ctgcagtgct gtttgtgttt ttcgtaagcg
     3001 cagtatcgat cttcatggct tcgacatctg tgtatgctac caatggtatg aatcttgagg
     3061 gttacggtcc gatcgcgacc ggcatgggcg gcgcgtccat ggcctacgac aacgggactg
     3121 cagcagtcat gaacaacccc gccaccttag gcctgatgcc ggaagggaac cggctcgacg
     3181 tagcgctcgg ctacctgggg ccccatatca aggctacctg caatgccggg ccgtgtgcgg
     3241 gagcggatgc gtcgtcgtcc gcaacttcat tctggatgcc ggccatcggc tggatccaga
     3301 agtccggccc gttcgcctac ggcatcggtg tattcgcgca gggcggcatg gggacggaat
     3361 atggcgccga ctcattcctg gccttgggct cgggtgacaa ggtccgttcc gaactgggcg
     3421 tcggcagggc gatcatcccg ttcaccgttg acgtcaacaa gaatttcacg gttggtgtaa
     3481 ccgctgattt tgtctgggcc acgctggacc tgaaaatggc tctcccgggc actgaatttc
     3541 ttgcgatggc gcaggggacc agtgcattcg gatctgcaag cgggtctttg gtcaatagtc
     3601 tcggtggcgc ggtgcaggcg ggtcaaatcg gagtacccga gtggacgcgc tttgattttt
     3661 ccgataaaag cgattttacc ggtgcggcaa agggcaccgg ttttgccggc aagatcggcg
     3721 gcgtctacaa ggtaagtgac gccgtcagcg tcggggcgac gtatcacagc aaaaccgaac
     3781 tgggagacct ggaagcaacc ggcgcaacct tatcgatgaa ggcatcgggc ccggccacgg
     3841 gaggccaggc aatcgtcgta cccatcaccg gcaagatcca ggtaaaggat tttcagtggc
     3901 cgcagactat cggtgtcggc gtcgcctatc aggcaacgga caaactcatg atcgcagcgg
     3961 actataaatg gatcaattgg gccgatgtct tgaaaagctt caaactgacc tttacggccg
     4021 atgcaaatca gtcaggtgcg gccggccccg ggggctttaa tttaggggga cagtccgttg
     4081 acgccattct gttccagaac tggaagaacc agaacgtgtt cgaaatcggt gcgggatata
     4141 aatttacgcc tgagttcacc ggtcgcatcg gcttcaacta cgcgaacaat ccgattccgg
     4201 accagctcat gaacccgttg ttcccggcaa tcgaaaagag ccacgtgacc ataggagcgg
     4261 gctacatgat cacaaaggca tcgagtgtag atgcgtcgtt cacctatgcg ccggaagtga
     4321 aacagaccaa cggccagcag gtgaccgtcg cgcacagcca gaccaacgcg caggtcatgt
     4381 acagcttccg gttctaagtg aggaaagtaa acacatgaat tcatcaagag ggctacggga
     4441 gactgtagcc ctcttttttt gaaaaggagg cactatgaag tctctattgc gaaagctgat
     4501 gttgacggca ttattcatga tcctgctcgt aaatgcggcc ggcgccgagg aacttctcaa
     4561 acccttcatt ctcggatccc gcggccccgg ttccgtcgcg gaaaaagcgg acgtggtcaa
     4621 ggccgccctt acggggcagg gattcacggt tgccggggaa tactcgccct atcccgacgc
     4681 cgtcgtcatc gtggcgacga acgacgaact gaagaatacg gcggccaaat cagagcgcgg
     4741 cggtttcggc gccgcggtcc gcgtctcggt taccaaggta aaggacgagg tgcaggtggc
     4801 gtacacgaac cccgtgtaca tggccaacgc ataccgtatg cagggcgact tggtggacgt
     4861 cgccgccaaa ctcgcggccg cccttggcaa aaaagaggat ttcggggcaa agggcctcac
     4921 agccaagaaa ctccgctcct accactacac ggtcggcatg gagtatttcg atgagcccag
     4981 cgtgctcgcg gaatacaaga gcttcgaaga ggctgcggca gccgtcgaag caggccttgc
     5041 ggcgggaaaa gggggcgtta cgaaagtgtg ccggatcgac atcccgggaa agaaagaggc
     5101 ggtattcggc gtaggaatga aggccgcctc agacgccgac aagtttatgg acgacaagtt
     5161 catcatgagc gaaatagatt tcaaggacct caaatccacg gcgcacctgc cctatgagat
     5221 gctcgtttcc ggcaacaagg tctacgcgct gtacgcccgg ttccgcatcg ccatcagttt
     5281 cccggaccta tccatgatgg ggagcaacag cttcatgcac atcatggaca gtcccaaggc
     5341 aatccgcaag gccctgaccg aggccgcggg agggaagatg taaagcaact aattttgtca
     5401 ttcccgaatg tttctatcgg gaatccattt tgaatttact ttcgaaaaag cctatggatt
     5461 cccgctttcg cgggaatgac aaacggggaa gcttaacaac tgcatctcta tgttaaacct
     5521 cctccctgcc gggaggaggt tttttatgcg gctgcgatca tggcattgtt tcgtggttga
     5581 ctgtgtaaga cggatggatt atgatcacgg cacaagccta caatgaaaat tcttatcata
     5641 aaactttccg ccatcggcga tgtggcgcag accctgcccg ctcttgaagc gctcaagaaa
     5701 atccatcccg ctagtgaaat cacctgggtt gtcgaagagg cagccgcggg cgtcctcgaa
     5761 ggccatccgc tcatcgaccg cctgctggtc tcccgcagga aagcgtggtt ccggtcgctg
     5821 atgaatccgc tcgtgctcgc gcggggcatg ggggacatgg tccggttcgt ccgcgagctc
     5881 cgccgaacgc ggtacgacat cgcaatagac ttccagggtc ttctgaaaag cgggatcgtg
     5941 atcggccttg cgcgggccga ccgcaagata ggcttcgacc gcacgcggga actttcctat
     6001 cttttcctga acgagcgcct gccggcctat gacgtcgagc ggcatgcgct cgaacgttat
     6061 ctggacgtcg cacggtatct cggcgcgaag gacccgtcaa catcgtgtct gctgccgctt
     6121 gaaaaggagc gtccggttat ttcgaagcgg gtcggagaga ttagggcgcc gggacggctg
     6181 ctcgtggccg tgaacccggt cgcgcgctgg aagacgaaac tctggcagga acggaagttt
     6241 gcggagttgg cggaccgcct gattcgggaa aagaacgccg tcgtgatctt cacgggaagc
     6301 cccggcgacc ggcagg
//
LOCUS       DASSLD010000265         5920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1048288, whole genome shotgun sequence.
ACCESSION   DASSLD010000265 DASSLD010000000
VERSION     DASSLD010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048288"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /gene="ligA"
                     /locus_tag="VFK23_04775"
     CDS             complement(<1..1080)
                     /gene="ligA"
                     /locus_tag="VFK23_04775"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET7318433.1"
                     /translation="MEKIPADIKNEIEKLVKELNEHNYRYYVLDAPVISDEAYDRMFR
                     RLQELEEKYSYIPPDSPTQRVGAPPLDKFEKVRHTELMLSLDNAFSHDEVREFDQRVK
                     RFLKSDREVEYTVEPKYDGLAMELTYKNGVLDHASTRGDGIEGEDVTRNIRTIKAIPL
                     VIAGVDVPQEIDIRGEVYMDIDEFEKMNREREKEGEAAFANPRNAAAGSIRQLDSSIT
                     EKRKLHMACYGAGAMKGIEFKSQAEFVTWLKKARFPVPAVFDVVTGIDKAVESIKKIE
                     EKRPAFPFETDGAVVKVNDFRLQKELGIKTREPRWAIAYKFPAHQGTTVIEDIIASVG
                     RTGAITPIAMLRPVRIGGVTVSRSTL"
     gene            complement(1216..2598)
                     /locus_tag="VFK23_04780"
     CDS             complement(1216..2598)
                     /locus_tag="VFK23_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7318434.1"
                     /translation="MDTILIVEDKESMLDMLKQTLEGEGYNIITARDGAEGIKKLADE
                     RIGVVLTDLKLPKKDGFEVLRAVKQESPLLPVIVMTAFGTIETAVKAVKEGAYDFLTK
                     PFDTDHLLVLIKRGLDTTRLAAENILLREEFAEKLGMPKIIGKSVQMQDITAKIQKVA
                     PTNATALIHGESGTGKELFARAIHYLSARKDAPFIAINSAAIPRELLESELFGYERGA
                     FTGADARKLGKLELADKGTVFLDEIGDMDMALQAKLLRVLQEQVIERVGGGKPIKIDV
                     RVVAATNKDIEKAVAGKLFREDLFYRLNVFPITIPPLRERREDIPALAQNFLNKYSAE
                     VRKGPLTISDEAMEILMKSPWKGNVRELENVVERAVIYADGGVIRAQDLGISESSIID
                     ALAEHVPMDGPLQDVAAAATRIAESRRIRQVLKQTGGNKSRAAEILQVSYKTLLTKIK
                     DYQLEDKGDS"
     gene            complement(2620..3882)
                     /locus_tag="VFK23_04785"
     CDS             complement(2620..3882)
                     /locus_tag="VFK23_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7318435.1"
                     /translation="MTGQTLLILLPGILAVIIAAIAIWSFMRRYRRELGVAKEQREKT
                     TDTSFIINAFHEVTKQLKEKEKELERLKSIAEQHAENIESYNENILQCVTSGVMTFDA
                     ECRLTTINRAAEETLSLGKDDVIGKTCLELFGHNEISRAVRNTLEGHVPSARMEDRLE
                     RSGGGLWLGFNTAVLADRQNEVIGVILSFTDLTEVKRLQAQMELRERLTALGEMSAGI
                     AHELRNPMAVISGYLNLLSKKTDQAGQEVTRKIAEEISGMNRIIGDLLTFARPASLNR
                     ARVNVKELLEGCLASARQAAAPDQPFETKVRIEEIEASVDEVLMRQAFTNLIQNALEA
                     MPGGGTVFLEAGMRNDELAVVIRDTGTGIPPAIVKKIFLPFYTTKDKGVGMGLALAHK
                     VITSHGGRIEVESKEGKGTTFTVMLPRG"
     gene            complement(3879..4706)
                     /locus_tag="VFK23_04790"
     CDS             complement(3879..4706)
                     /locus_tag="VFK23_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET7318436.1"
                     /translation="MITAAAFAFGLVIGSFLNVCIVRLPRDLSVVKPSSHCPVCKTPI
                     RFYDNIPIISFLMLRANCRSCGEPISWRYPLVELMNGLLYVWIVREFPAGGEALLIMA
                     FCSSLIVITFIDLDHQIIPDVITLPGMLVGLALAPFFMYPLADPLPFHLGEFVPHAGR
                     YLTGFLNSLIGLVLGGAPLLAIGWIWEKLRHVEAMGGGDVKFMAMVGSFFGWKGALLT
                     IMLGALSGSVVGVLLIMLKRHKMEKVIPFGPFLAAGALTNAFYGPDIISWYLGLMRL"
     gene            complement(4703..5071)
                     /locus_tag="VFK23_04795"
     CDS             complement(4703..5071)
                     /locus_tag="VFK23_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318437.1"
                     /translation="MTIWEKTVLNIEKGGRKLTVAAATFSEWAKAELAVIRTRIRIDE
                     AQRGIEELQRQIGRKITDLRKQDALPKSTEQLLKDEAISIALAEIADREQELEGLMTE
                     LKSIRIDYKTAVKQTEDTLP"
     gene            complement(5312..>5920)
                     /locus_tag="VFK23_04800"
     CDS             complement(5312..>5920)
                     /locus_tag="VFK23_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET7318438.1"
                     /translation="SDLKAATEMASHLQKDVFPEWKVGLLHGRLKAGEKEAVMAAFKA
                     GETHILVSTTVIEVGVDVPNASVMVIEHPERFGLAQLHQLRGRVGRGSYQAYCILMGP
                     RMFAEEARERLTAFARTTDGFRIAEEDLRQRGPGEFFGTRQSGLPDLRAANIIRDADL
                     LEQARFEAFGLLKQDPQLAFHPLLRELLHRKWQGRLGLISVG"
BASE COUNT         1070 a         1831 c         1680 g         1339 t
ORIGIN      
        1 cagcgtggat cgtgaaacgg taaccccgcc tatccgcacg ggccggagca tggcgatcgg
       61 cgtgatcgcc ccggtccttc ccacgctcgc gataatgtcc tcgatgaccg tagtcccctg
      121 gtgggcgggg aatttatagg ctatggccca tcgcggctcc cgggtcttga ttcccagttc
      181 cttctggagc ctgaagtcgt tcaccttgac gaccgccccg tccgtttcaa aggggaatgc
      241 cggcctcttc tcttcgatct tttttatcga ttctacggcc ttgtcgattc ccgttacgac
      301 atcgaaaacc gcaggtacgg ggaagcgggc ctttttgagc caagtcacaa actccgcctg
      361 gctcttgaat tctataccct tcatcgcacc ggcgccgtaa caggccatgt gaagcttccg
      421 cttttccgtt atcgacgagt caagctgccg gatcgaaccc gctgcagcgt tccggggatt
      481 cgcaaaagcg gcttctcctt ctttttcccg ctccctgttc attttctcga actcgtcgat
      541 gtccatatac acttcgcccc ggatgtcgat ttcctgaggg acatcgacgc ccgcgatcac
      601 gagcgggatc gccttgatcg tcctgatgtt ccgtgtgacg tcctccccct cgatcccgtc
      661 tccgcgcgtc gaggcatgat cgagtacgcc gttcttatag gtcagttcca tcgcaagacc
      721 gtcgtattta ggttcgaccg tgtattcgac ttcccggtcc gatttcagga accgcttcac
      781 cctctggtcg aactcccgga cttcgtcgtg tgagaacgcg ttgtcgagcg agagcatgag
      841 ctcggtatgt ctcacttttt cgaacttgtc gagaggcggc gcgcctacgc gctgcgtggg
      901 agaatccgga gggatatagc tgtacttctc ttcaagctct tgcagtcgtc ggaacatccg
      961 gtcatacgct tcgtcggata tgaccggcgc gtccaggacg taatagcggt agttgtgctc
     1021 gttcagttcc ttgacgagct tttctatttc attcttgata tcggcgggaa tcttttccat
     1081 gatgtcatga accaggcgga atgaggcgat tttgtgaaat caaatcgaaa agagacgatt
     1141 ccgggcaagc cggaatgaca acaaaaaatg ctcctgcgag acctcggtac cctgaaccgt
     1201 ggaagactct gaacgttaac tgtctccttt atcctccaac tgatagtcct tgatcttggt
     1261 gaggagcgtc ttgtagctca cctggaggat ctccgccgcc cgcgacttgt tcccgccggt
     1321 ctgtttgagg acctgcctta tgcgacgcga ctccgcgatg cgcgttgcgg ccgcggccac
     1381 gtcctgcagg gggccgtcca tgggcacatg ctcggcgagg gcatcgatga tgctcgactc
     1441 gctgatcccg aggtcctgag cgcggatcac cccgccgtcc gcatagatca cggcgcgctc
     1501 gaccacgttc tcgagctccc ggacattgcc cttccagggg gacttcatca ggatttccat
     1561 ggcttcatcg gagatggtca gcggcccttt cctgacctcg gcagaatact tgttcaggaa
     1621 gttctgggca agggccggaa tgtcctccct ccgttccctg agcggcggga tcgtgatggg
     1681 gaacacgttc aggcggtaga acaggtcctc ccggaacagc ttgccggcga ccgccttctc
     1741 gatgtccttg ttcgtcgctg ccacgacccg cacgtcgatc ttgatcggtt tgccgccgcc
     1801 cacgcgctcg atcacctgct cctgcagcac gcgcaagagc tttgcctgca gcgccatgtc
     1861 catgtcgccg atttcgtcga ggaagactgt gcccttgtcc gcaagttcga gtttgccgag
     1921 tttcctcgcg tccgcccccg tgaaggcgcc gcgctcgtac ccgaagagtt cgctttccag
     1981 cagttcgcgg gggatggccg cggaattgat cgcgatgaac ggggcgtcct tgcgggcgga
     2041 gaggtagtgt atggcgcggg cgaacaactc cttgcccgtc ccgctctcgc cgtggatcaa
     2101 tgcggtggcg ttcgtcggcg ccaccttctg gatcttcgcg gtgatatcct gcatctgtac
     2161 gctcttgccg atgatcttgg gcatgcccag cttctcggcg aattcctccc tgagcaggat
     2221 gttctccgcc gcgagacgcg tcgtgtcgag ccccctcttg atgagcacca gcagatgatc
     2281 ggtgtcaaag ggcttggtca gaaaatcgta cgccccttcc ttgacggcct tcaccgctgt
     2341 ctcgatggtc ccgaaggcgg tcatgacgat aaccggcagg agcgggcttt cctgcttcac
     2401 cgcgcgaagg acctcgaagc cgtccttctt gggcagcttg aggtccgtca gtaccacgcc
     2461 gatgcgctcg tcggcgagct tcttgattcc ctccgcgccg tccctggccg taatgatgtt
     2521 atacccctcg ccttccaggg tctgtttgag catgtcgagc atgctctcct tgtcttcgac
     2581 gatcaatatg gtgtccatgt tcacctatcc gggaaacaat catcctctcg gcagcattac
     2641 cgtaaacgtc gtccccttcc cttccttgct ctctacttct attctccccc catgggaggt
     2701 aatgacctta tgcgccagag cgagtcccat gccgacaccc ttgtccttgg tcgtatagaa
     2761 cgggagaaat attttcttga cgatcgccgg aggaatgccc gtccccgtgt ccctgattac
     2821 gacggccagt tcgtcgtttc tcatcccggc ctccagaaaa acggttcccc ctccgggcat
     2881 ggcctcgagg gcgttctgga tgaggttggt aaatgcctgt ctcatcagca cctcgtcgac
     2941 ggaggcctct atctcctcga tccggacctt cgtctcaaag ggttgatccg gcgctgccgc
     3001 ctgccgggcg cttgccagac agccttccag gagttccttg acgttcaccc gcgcgcggtt
     3061 gagcgacgcc ggccgggcaa aggtcaggag atctccgatg atgcggttca taccgctgat
     3121 ttcctctgcg attttccgcg tgacctcctg gcccgcctga tcggttttct tggagagcag
     3181 gttgaggtag cccgaaatta ccgccatcgg gttccgcagt tcgtgcgcga tgccggcgga
     3241 catctcgccc agggccgtaa gccgctccct gagctccatc tgcgcctgca gccgcttgac
     3301 ttccgtaagg tccgtaaacg agaggatgac cccgatcact tcgttctgcc ggtcggcgag
     3361 aacggccgtg ttgaagccga gccacaggcc gccgccgctg cgttcgagcc tgtcctccat
     3421 gcgtgccgac gggacgtggc cctcgagcgt gttacgcact gcgcggctga tctcattgtg
     3481 gccgaacaat tcgagacagg tttttcctat tacgtcgtcc ttgccgagac tcagcgtttc
     3541 ctcggccgcg cgattgatcg tcgtcagcct gcattcggca tcaaacgtca tcaccccgct
     3601 ggtgacgcac tgaaggatgt tttcattgta gctttcgatg ttctctgcgt gctgctccgc
     3661 gattgacttt agccgttcga gctccttctc tttttccttg agctgcttgg taacttcatg
     3721 aaaggcgttg atgatgaacg aggtgtccgt cgttttttcg cgctgctctt tcgcaacgcc
     3781 gagttcccgg cgataccgcc gcatgaaact ccagatcgcg atggccgcga taataacggc
     3841 cagtatcccg ggcagcaata tcagcagtgt ctggccggtc ataagcgcat caggccgagg
     3901 taccacgaga tgatgtcggg tccgtaaaag gcgttcgtca gcgcgccggc ggcgagaaag
     3961 gggccgaagg ggatcacctt ctccatcttg tgccgcttca gcatgatcag caggacgccg
     4021 acgacggatc cggagagcgc accgagcatg attgtaagaa gggcgccttt ccagccgaag
     4081 aagctcccga ccatcgccat gaacttgaca tcgccgccgc ccatcgcttc gacgtgcctg
     4141 agtttttccc agatccagcc gatggccagg agcggggcgc cgcccagcac aagaccgatg
     4201 agggaattga gaaacccggt gaggtaccgt cctgcgtggg ggacgaactc tcccagatga
     4261 aacggcagcg ggtcggcgag gggatacatg aaaaacgggg caagggccag ccccacgagc
     4321 atgcccggga gggtgatgac gtcggggatg atctggtgat ccaggtcgat gaacgtgatc
     4381 acgatcagcg acgagcagaa cgccatgatc aggagcgcct ccccacccgc cgggaattca
     4441 cggacaatcc agacgtagag aaggccgttc atgagttcga cgagcggata gcgccacgag
     4501 atcggctcgc cgcagctccg gcagttggcc cggagcatga ggaaactgat gatcgggatg
     4561 ttgtcgtaaa atcgtatggg cgtcttgcaa acggggcagt gcgaggacgg cttgacgacg
     4621 gaaaggtccc gggggaggcg tacgatgcag acgttcagaa aacttccgat gacgagcccg
     4681 aacgcgaaag cggcggcggt gatcatggga gagtgtcctc cgtctgtttc accgcggttt
     4741 tgtaatcgat gcggatgctc ttcaactccg tcatcagccc ctcgagctcc tgctcccggt
     4801 cggcgatttc ggccagcgcg atgctgatgg cctcatcctt cagaagctgt tccgtggact
     4861 tcggcagcgc gtcctgtttc ctgaggtccg tgattttccg tccgatctgg cgttgcagtt
     4921 cttcgatccc ccgctgcgcc tcgtcgatcc tgatccgggt ccggataacg gcgagctccg
     4981 cctttgccca ctccgaaaaa gtggcggccg caaccgttag cttccggccc cccttctcta
     5041 tattcaggac cgttttttcc cagatggtca taataaaacc tcaatgcgga atgtgaaatt
     5101 cggatggccg atgaaacggg ccacgccgat gcggaatgaa cctcttcccc tatccgcatc
     5161 agtctgtacc ggtaaccgaa tcgtggacga ggagcccatt ttcgacgaaa ataatgcgcc
     5221 ggcttttata acgctgtgca gatttaattc cgaattccac attccaaatt ccgcatctgt
     5281 accgctactc tatcagaata cctcaaaaat atcaaccaac gctgatgagc cccagcctcc
     5341 cctgccactt cctgtggagc agttcgcgga gcaggggatg aaaggcgagc tgcgggtcct
     5401 gcttcaggag tccgaaggcc tcgaaccttg cctgttcgag caggtccgcg tcgcggatga
     5461 tgttcgccgc gcgaaggtcc ggcaggcccg actggcgcgt tccgaagaat tcgccgggac
     5521 cgcgctggcg cagatcctct tcggcgatcc tgaaaccgtc ggtggtgcgc gcgaaggcgg
     5581 tgagccgctc cctggcctct tccgcgaaca tccgggggcc catgaggatg cagtacgcct
     5641 gatagcttcc cctgcccacc ctgccgcgaa gctggtggag ctgggcgagt ccgaagcgct
     5701 cgggatgttc gatcaccatg acgctcgcgt tcggcacgtc cacgccgacc tcgatcaccg
     5761 tggtcgatac caggatgtgc gtctcccccg ccttgaaagc ggccatgacc gcttccttct
     5821 cgcccgcctt cagccgtccg tgcagaagtc ccaccttcca ttccggaaag acatctttct
     5881 gaagatggga cgccatctca gtcgcggcct tgaggtcgct
//
LOCUS       DASSLD010000266         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_681067,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000266 DASSLD010000000
VERSION     DASSLD010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681067"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..2146)
                     /gene="fusA"
                     /locus_tag="VFK23_04805"
     CDS             complement(89..2146)
                     /gene="fusA"
                     /locus_tag="VFK23_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET7318439.1"
                     /translation="MGIMAHIDAGKTTTERILYYTGVSYKIGEVHDGTAVMDWMVQEQ
                     ERGITITSAATTAFWRDFRINIIDTPGHVDFTIEVERSLRVLDGAVAAFDSVSGVEPQ
                     SETVWRQADKYHVPRLAFMNKMDRIGADFYMSVQSMIDRLGANPVPIQIPVGAEDKFR
                     GCVDLVKMKSVLFDDETLGAKYIVEDISADLLPKAKEYREKMIEKLADVDDVIMEKFL
                     NGEQPTAAEIMAAIRKGTIAMKIFPVICGSAFKNKGVQLLLDAVVDYLPSPLDIPPVK
                     GVNPVSGEELVRNASDSEPFAGIAFKIMTDPFVGQLTFVRVYSGTLKTGSYVYNSTKS
                     TRERVGRLLKMHANKREEIQEIYAGDICAAVGLKSTLTGHTLCDENKPVILEAMTFPE
                     PVISVAIEPKTKADQEKLGFSLQKLMQEDPSFRVFTDQETGQVIISGMGELHLEIIVD
                     RLLREFKVDANVGKPQVAYRETIRKKVTAEGKYVRQTGGRGQYGHAVLELEPQEPGKG
                     FEFINKVVGGAVPKEYVAPIQKGIVEAMENGVLAGYPMVDIKVTLIDGSYHDVDSSEM
                     AFKIAGSMGFKEGANKASPALLEPIMAVEVVCAEEYMGDIIGDLNSRRGRVQGMNMRG
                     AGRVITAQVPLSEMFGYATDIRSKTQGRATYTMQFAHYEEVPKNISEQIIAKVKGETV
                     KAK"
BASE COUNT          443 a          646 c          535 g          523 t
ORIGIN      
        1 catgaggttt cgtcctctca aatttcgcct ttgccatgct gtccgcctcc tgtcgggaag
       61 tgaaaaaata atccagtaga aaccatgact actttgcctt aaccgtctcg ccttttacct
      121 tggcgatgat ctgctccgaa atgttcttgg ggacttcttc gtagtgcgcg aattgcatcg
      181 tgtacgtcgc ccggccctgg gtcttggacc tgatgtccgt tgcgtagccg aacatttccg
      241 agagaggcac ctgcgctgtt atgacccttc cggcccccct catgttcatg ccctgcactc
      301 taccgcggcg agaattcagg tcgccgatga tatcgcccat gtactcttcg gcacagacaa
      361 cctcaactgc catgatcggc tcgagcaaag ccggcgaagc cttgtttgct ccttctttaa
      421 aacccatgga accggctatt ttaaatgcca tttccgacga atccacgtca tgataggacc
      481 cgtcgataag tgtaaccttg atatcaacca tgggataccc ggcgagcact ccattctcca
      541 tggcctctac gatacccttc tgtatcggcg caacgtattc cttaggaaca gctcccccaa
      601 ctaccttgtt gataaactca aaaccttttc ccggttcctg aggttcaagc tcgagtaccg
      661 catgaccata ctggccgcgg ccgccggtct ggcgaacgta cttgccctct gcagtaacct
      721 tctttcggat cgtttcgcga tacgcgactt gcggctttcc aacgttcgca tctaccttga
      781 actcacgcaa gaggcggtca acgatgattt ccagatgaag ctctcccatc ccggagataa
      841 tgacctgccc cgtctcctga tcagtaaaaa cacggaatga cgggtcttcc tgcatgagct
      901 tctggagcga gaaacctagc ttttcctggt ctgccttggt cttgggctcg atggccacgg
      961 aaatcaccgg ctccgggaat gtcatggctt ccaggatgac gggtttgttt tcatcacaaa
     1021 gcgtatgccc cgtgagcgtg ctcttgagtc ccaccgccgc gcagatatcg ccggcatata
     1081 tctcctggat ctcttcgcgt ttgttcgcgt gcattttgag gagcctgcca acacgctccc
     1141 tggtactctt ggtagagttg tacacatacg acccggtttt cagcgtccca gaataaaccc
     1201 ggacgaacgt gagttggccc acgaagggat cggtcatgat cttgaacgct atacccgcaa
     1261 agggctcgct gtcagaagca ttgcgcacca gttcctcacc gctaaccggg ttgacgcctt
     1321 tcacaggcgg aatgtccaaa ggagacggga ggtagtccac gaccgcatcg agcaggagct
     1381 gcaccccttt gtttttaaat gccgagccgc atatcacggg gaatatcttc atggcgatcg
     1441 tgcccttgcg gatagccgcc atgatctctg ctgccgttgg ctgctctccg ttcaggaact
     1501 tttccatgat tacgtcgtcg acgtctgcga gcttctcgat catcttctcg cggtactctt
     1561 ttgccttggg caagagatcc gcagagatgt cttccactat atattttgca ccaagggtct
     1621 cgtcgtcgaa caggacagac ttcatcttga ccaggtcaac gcagccgcga aacttgtcct
     1681 ctgcgccgac agggatctga ataggcacgg gattggctcc gagccggtcg atcatcgact
     1741 ggacgctcat gtagaagtct gcgccgatgc ggtccatctt gttcatgaaa gcaagacggg
     1801 ggacatgata tttatccgct tgacgccaca ccgtttctga ctgaggctca acgccgctca
     1861 ccgagtcgaa ggccgcaacc gctccgtcga gcacgcggag cgaacgctcc acctcaatcg
     1921 taaagtccac atggcccggc gtatcgatga tgttgatgcg gaagtcgcgc cagaacgccg
     1981 tcgtcgccgc cgaggtaatc gtgatgccgc gctcctgctc ctgcaccatc cagtccatga
     2041 ccgccgtacc gtcgtgcact tcgccgatct tgtaggaaac accggtatag taaaggatcc
     2101 gctcggtcgt cgtcttcccg gcgtcgatat gagccatgat cccgatg
//
LOCUS       DASSLD010000267         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_196522,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000267 DASSLD010000000
VERSION     DASSLD010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196522"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..523
                     /locus_tag="VFK23_04810"
     CDS             119..523
                     /locus_tag="VFK23_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HET7318440.1"
                     /translation="MAIQWTEDLATDVPEIDMQHKELFSRINRLLEACNQGQGRSEVG
                     KTLAFLEEYVLIHFATEEKIMTGRLYPEYAGHKAQHVQFMSNLDALKQQFAKEGPTVH
                     LTLLTNHTVVDWLRNHIKKLDRDLGAFLKKRT"
     gene            793..1407
                     /locus_tag="VFK23_04815"
     CDS             793..1407
                     /locus_tag="VFK23_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:961..963,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET7318441.1"
                     /translation="MSEHCCGIRVGQAVPDFKLETFDPSKGDFGEVSLAAQKAAKKWT
                     VLFFYPADFTFVUATEFAALAEQYDEFKKAGADVITVSTDTKFVHLAWQRDEKMLEKV
                     KYPMGSDRTGKVARMFGVYDDETGHALRGTFIISPDGTLLNSEVNFYNMGRNVEEILR
                     KINANVHLASSPAEACPAQWRKEGDKTLKPSAAMVGRVSVAMKK"
     gene            1409..1828
                     /locus_tag="VFK23_04820"
     CDS             1409..1828
                     /locus_tag="VFK23_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET7318442.1"
                     /translation="MLITRETDYAVRTVLYLARNRDRTANVTEVAHAMHIPKSFLAKV
                     LQRLVRHHILTSMRGVGGGFRLAKKPSEISLLAIMEAVQGPAAINVCAIDSKRCSLSA
                     TCSVHPVWVDIRQELEKRLRRQTMDKLAGNKKSPAGK"
     gene            1864..>2368
                     /locus_tag="VFK23_04825"
     CDS             1864..>2368
                     /locus_tag="VFK23_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET7318443.1"
                     /translation="MNDYQYDDQTVKGFIVSSIFWGVVGIVIGLWISVQMWLPSWNLP
                     PYFTFGRMRVVHTNGLAFGLGVGAIFGVSYYIVMRLCKRPLLFPKLARFHLWLFNAAI
                     ALAALSLFAGMNQSLEYAELEWPLDVGVVILWVIFAVIIFGTVIKRKEEQMYISLWFI
                     LATVVAVA"
BASE COUNT          545 a          682 c          635 g          506 t
ORIGIN      
        1 tggagtttcc gatcaccgct tgacaatggt ttcttccgtg gtacattaaa aaaaaaatgc
       61 tcacacgcgt cctgccggtc cgggcgacgc atccgaaggc aaccgcagag gaggcttcat
      121 ggcgattcaa tggacggaag atctggcgac cgatgtaccg gaaatcgaca tgcagcataa
      181 agagctgttc agccgcatca accggctgtt ggaagcctgc aatcaaggcc agggccggtc
      241 cgaggtcgga aagaccctcg cgtttctcga agaatacgta ctgatccatt tcgccacgga
      301 ggagaagatc atgaccgggc gtctctaccc cgagtacgcg ggacacaagg cgcagcacgt
      361 tcaattcatg tcgaacctcg acgccctgaa gcagcagttc gcgaaggaag gccctaccgt
      421 gcatctcact ttgctcacca atcatacggt agtcgactgg ctccggaacc atatcaagaa
      481 gctcgaccgc gacctcggtg ccttcctgaa gaaacgcacg tgacgatatt cctattttcc
      541 cctttcttcg aaactttccc ccccgtccac cccttctact gtgtgtgata tactgatcta
      601 ttgggaacaa tgaaatagcc tggctttttt tagaaaagcc caaaccgcgt cacgtgatgg
      661 ctccggattg cgcgtctccg caacttccgg ctactttatg atttttcaca aaatcgctat
      721 tgacaaattt tcttaaagtg ataatttagg gtctgaaata tcctaattac tcttccccag
      781 gaggtaagaa ccatgtctga acattgctgc ggcatccgag tcgggcaggc cgtgcccgac
      841 ttcaaactcg aaaccttcga tccctcgaag ggcgatttcg gcgaagtaag cctcgcggcg
      901 cagaaggcgg cgaagaagtg gaccgtcctc ttcttttacc ccgccgactt tacctttgtc
      961 tgagcgacgg aattcgctgc tctggcggag cagtacgatg aattcaagaa ggccggtgcc
     1021 gatgtcataa cggtcagcac ggacacgaaa ttcgtgcacc ttgcttggca gcgcgacgaa
     1081 aagatgctcg aaaaagtcaa gtacccgatg ggttccgacc gcacgggaaa agtggcgcgc
     1141 atgttcggcg tctatgacga cgaaacaggc cacgccctgc gcggcacctt catcatcagc
     1201 cctgacggca ccctgctcaa ctccgaagtc aatttctaca atatgggaag aaacgtcgaa
     1261 gaaatcctga gaaagataaa cgcgaacgtg cacctcgcct cctctcccgc cgaagcctgc
     1321 ccggcgcagt ggagaaagga aggagacaag acgctgaagc cttccgcagc gatggtcggc
     1381 agggtttccg tggcgatgaa gaagtaatat gctaataacc cgagaaacgg attacgccgt
     1441 ccgcacggtc ttgtatcttg cgcggaaccg ggaccgtacc gcgaacgtca cggaagtggc
     1501 ccacgcgatg cacataccga agagcttcct tgccaaggtc ttgcagaggc tggtgcgcca
     1561 ccatattctt acctcgatgc gcggcgtggg cggcgggttc cgtcttgcaa aaaaaccgtc
     1621 ggagatcagc ctgctcgcca tcatggaggc cgtgcagggg cctgcagcaa tcaacgtctg
     1681 cgccatcgac agcaaacggt gcagcctgag cgcaacctgt tccgtccacc cggtatgggt
     1741 ggacatccga caggagctgg aaaagcggct caggagacag accatggaca aacttgccgg
     1801 taacaagaaa tcgccggccg ggaaatagga ccccaccagt ccttcacgta aggaggcaac
     1861 agtatgaacg attatcagta cgatgatcag acagtgaagg gtttcatcgt ctcctcgatc
     1921 ttctggggcg tggtcgggat tgtgatcggg ctctggatct cggtgcagat gtggctgccg
     1981 tcgtggaacc tcccgcccta tttcaccttc ggacgtatgc gcgtcgtgca cacgaacggg
     2041 cttgcattcg ggctcggggt cggcgcgata ttcggggtgt cctattacat cgttatgagg
     2101 ctctgcaaga ggcctctcct ctttccaaaa cttgcgcggt ttcatctctg gctctttaac
     2161 gccgccattg cgctggcggc cctctccctc ttcgccggaa tgaaccagtc cctcgaatat
     2221 gccgaactcg aatggcccct cgacgttggc gtcgtgatcc tctgggtcat tttcgccgtc
     2281 atcattttcg gcaccgtcat caagcgcaaa gaggagcaga tgtacatctc cctctggttc
     2341 atccttgcga cggtcgtcgc cgtcgccg
//
LOCUS       DASSLD010000268         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1452485, whole genome shotgun sequence.
ACCESSION   DASSLD010000268 DASSLD010000000
VERSION     DASSLD010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452485"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFK23_04830"
     CDS             complement(<1..294)
                     /locus_tag="VFK23_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HET7318444.1"
                     /translation="MKKQRVIIMGAAGRDFHNFNMVYRGSPDFEVVAFTAAQIPMIGG
                     RLYPPGLAGPLYPSGIPIRPEEELPELIRKFRVDQVAFSYSDVSHEYVMHKASL"
     gene            582..1226
                     /locus_tag="VFK23_04835"
     CDS             582..1226
                     /locus_tag="VFK23_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010295724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7318445.1"
                     /translation="MKRLLMVLSILTLLAAIALPVQAKSHEKAGTPHLTQSSVDLRMA
                     MRQLWEEHVMYTRNYIISAVAGLEDAGAVAERLLRNQDDIGNAIKPLYGEEAGKKLSA
                     LLRDHILIAADIVKAAKAGDNDGVAAGQKRWTANADEIAAFLSGANPKWSQQTLKDML
                     YKHLALTTTEVVSRLKKDWAADIEAYDKGHVHMLMFADVLTDGIVKQFPKKFSK"
     gene            1307..1504
                     /locus_tag="VFK23_04840"
                     /pseudo
     CDS             1307..1504
                     /locus_tag="VFK23_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
     gene            complement(1636..2886)
                     /locus_tag="VFK23_04845"
     CDS             complement(1636..2886)
                     /locus_tag="VFK23_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7318446.1"
                     /translation="MVVNPPTGPITAYRRYWLPELVVLAAAALGSIILFAATDLDITT
                     IRPFYHPDLAEPWPVANHPVWSLFYQSAPWVTASLALAGTALSVAGSLREQSKRLRLY
                     GIFILLCVVVGPGLVINVALKDHWGRPRPRQITEFGGRLDYAQPFVPTGAYGKSFPCG
                     HCSVGYLYAAGWWVWRRRRPALDVVSLVCGLVLGTLLGIGRMAAGAHFLSDAVWSALL
                     AYGIAHALYYYALRVPAREDGRAAVYPLILGSRHLKTTVIAGAVLTGLLIIGGGMLAN
                     PYFHELRACEPPAGFPPPRSIEILVDQLDVELHLIADAEGGISCSGTVHGFGLPGNGI
                     DSKAEFRMGPVPVLRYRVLLEGWFTDIDGVGSFRVPVREVRSIMVRAGRGDITVVDET
                     GGHLTAKEGPALDLHTVRGRVRYR"
     gene            3095..>3293
                     /locus_tag="VFK23_04850"
     CDS             3095..>3293
                     /locus_tag="VFK23_04850"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET7318447.1"
                     /translation="MVVGGGPAPGINGVISAVTYEAAKQGLDVVGVHDGFKWLSKGDT
                     GHATNLCKSDISRIHMRGGSVI"
BASE COUNT          741 a          938 c         1024 g          590 t
ORIGIN      
        1 gagcgatgcc ttgtgcatga cgtactcgtg cgacacgtcg ctgtaggaaa aggcgacctg
       61 gtccacccgg aatttcctga tgagttcggg aagctcctct tcgggccgga tagggatgcc
      121 gctgggatag agggggcccg caagcccggg gggatagagc cgtcccccga tcatgggtat
      181 ctgggctgcg gtgaaagcca cgacctcgaa gtccggactg ccccggtaga ccatgttgaa
      241 gttgtggaag tccctccccg cggcccccat gatgatgacg cgctgttttt tcatgaccgt
      301 tcacccctgc cgccagttcg aaacgttccg aactcccgct ccctctcatc gctccccgca
      361 atgggcggtg tacggaaaca agaatagtga ctgtgctgag cagacttaaa ataactatac
      421 cgcgatcggt cattgtttgc cggtgatgga gttgctgatt cctgcggggg agatgcgcac
      481 gatccgggat tcttcactcc tcccccgcat tcgatgcatt ctgattcagg gatgctataa
      541 ttatcagaaa gcattcatct acacaaaaag gaggatctca catgaaacgg ttacttatgg
      601 tactttcaat tctgacgctg ctagcagcaa ttgccttgcc ggttcaggcg aagagccatg
      661 aaaaggcggg aacaccgcat ttgacccagt catccgtgga tttacgcatg gcgatgagac
      721 aactgtggga agagcatgtc atgtacaccc ggaactatat catcagtgcc gtggcaggtc
      781 ttgaagatgc cggtgccgtt gctgaacggc tgctcaggaa ccaggatgac atcggcaatg
      841 cgatcaaacc gctttacggc gaggaggcgg ggaaaaagct gtccgccctt ctccgggacc
      901 atattctgat cgcagcggac attgtgaagg ccgccaaggc cggcgacaat gacggcgtcg
      961 cggcaggcca aaagaggtgg accgcgaacg cggatgagat cgccgcattc ctgagcgggg
     1021 caaaccccaa gtggtcgcag cagacgttaa aagacatgct gtataagcac ctcgccctta
     1081 ctacgaccga ggtcgtatcg aggttgaaaa aggactgggc ggccgatatc gaggcgtacg
     1141 acaaagggca tgtacacatg ttgatgtttg ccgatgtgct gaccgacggc atcgtgaaac
     1201 aatttccgaa aaaattctca aaatagccgc gtaacttcac gaagagtggg gccctgacac
     1261 agagggcccc gctctttctg ttgatagtgc gagggaagtc aggaacatga taaagatgaa
     1321 acgcgtctac gatcctccgg cgccgggtga cggcaggcga atcctgatcg accggctgtg
     1381 gccgagaggc ctcaaaaaag aagatgcgaa aatcgacgag tggctgaagg aactcgctcc
     1441 gagcacctca cttcggaagt ggttcggtca cgatcctgaa aagtggggcg agttccggag
     1501 gcggccctca aggagtatat cgaggccgga atgagcggct ccgggaagaa agcctcataa
     1561 cgcggagtgc atgcagcctg tttcgggcat gatgagcggc ggctggagta attccgaaga
     1621 acgatagcgt tttttctacc ggtatcggac ccttccccgc acggtgtgaa ggtcgagcgc
     1681 gggaccttcc ttcgctgtca ggtggcctcc tgtctcgtct acgaccgtga tgtccccgcg
     1741 tcctgcgcgt accatgatcg acctcacctc ccggaccggc acgcgaaagc tccccacgcc
     1801 gtctatgtcc gtgaaccagc cttcgagcaa gactcgatac cgcaggaccg gaacgggccc
     1861 catccggaat tcagcctttg agtcgatccc gttgcccggc aggccgaagc cgtggaccgt
     1921 gccagagcag gaaatccccc cttccgcgtc agcgatcaga tggagctcaa catcgagctg
     1981 gtccacgagt atttcgatgc tgcgtggcgg aggaaagccg gccggcggct cacaggcccg
     2041 cagttcgtgg aaatacgggt tcgcgagcat accgcccccg atgatcagaa ggccggtgag
     2101 gaccgcgccg gcaatcacgg tcgttttcag atgcctgctt cccaggatga gcgggtagac
     2161 ggcggcgcgg ccgtcttcgc gcgccgggac ccggagcgcg tagtagtaaa gggcatgggc
     2221 aattccgtac gccaggagtg cggaccacac ggcgtcggac aaaaagtgcg cgcccgcagc
     2281 catgcgtcct attccgagca aggtgcccag gaccaggccg cagacgagag aaacaacgtc
     2341 cagcgccgga cggcgccgtc tccacaccca ccagccggcc gcatagagat atcccaccga
     2401 gcaatgcccg caggggaaag acttgccgta tgcaccggtc gggacgaagg gctgcgcata
     2461 gtcaagcctc ccgccgaact cggtgatctg ccggggccgc ggccggcccc agtggtcttt
     2521 cagggccacg ttgatgacca ggcccggtcc gacaacgacg cacagaagga tgaaaattcc
     2581 gtacagcctg agccgtttcg actgttcgcg cagggagccg gcgacagaca gggcggtgcc
     2641 cgccagggcc agggacgccg tcacccaggg ggccgactga tagaacaggg accagaccgg
     2701 gtggttcgcc acgggccagg gctcggcgag atcaggatga tagaacggtc gtatggtagt
     2761 gatatcgagg tccgtggccg cgaacaggat gatcgagccg agggcggcag ccgccaggac
     2821 gacaagttcg ggaagccagt agcggcggta agccgtgatt gggcctgtcg gcggattcac
     2881 aaccatgttt tcatcccctg ctgtaagtat agccctcaaa aaacagcatg gagaagtccc
     2941 ggtgcacggg acattcccat tttggctcga caaatgccct taaccgtatt gacaattccg
     3001 acctgatttg ttacagttgc gcgtgcgaac ggcacgagga taaatcaaaa aaacgcgaaa
     3061 ttaccaggag gcgattatgg caggcaagct cgcagtggtg gtcggtggag ggcccgcccc
     3121 ggggatcaac ggtgtgatca gcgccgtgac ctatgaggcg gccaagcagg gcctcgacgt
     3181 cgtcggcgtg cacgacgggt tcaagtggct ttccaagggc gacaccggcc acgcgacgaa
     3241 cctgtgcaag agcgacatct cgcgcatcca catgcgcggc gggtcggtca tcg
//
LOCUS       DASSLD010000269         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_716162,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000269 DASSLD010000000
VERSION     DASSLD010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716162"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFK23_04855"
     CDS             <1..435
                     /locus_tag="VFK23_04855"
                     /inference="COORDINATES: protein motif:HMM:NF028509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HET7318448.1"
                     /translation="VMAQSAVKDECVALCKDAAKFINEKGVDAAIAEIGNKEGKFVTK
                     NTYVFLMDLEGNRLAHPYARPEDPKIMKLFDQKDTTGKLFVQEYITVAKSKGEGWTEY
                     TYPTPEELKKPTPYKEKKSSKKISYVYRVPGKDLMVIAGFFE"
     gene            529..1038
                     /locus_tag="VFK23_04860"
     CDS             529..1038
                     /locus_tag="VFK23_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318449.1"
                     /translation="MKRWGLFLAMLLSVTVLLAPATQAADEVAGRVVYHTQKVEMMEA
                     GDVPGHIVGVLQQSGLTFYTKGPASGQIATRMMNTSYDVVKWIGSYTAYIVDSFQDGS
                     TLIYKASGTITPIDGGNRTAFEGTYEITGGAGRFEGKKGKGSFKGERIGSPKTGGDSY
                     ADFTGTEWK"
     gene            complement(1111..1557)
                     /locus_tag="VFK23_04865"
     CDS             complement(1111..1557)
                     /locus_tag="VFK23_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET7318450.1"
                     /translation="MRFMMMMIPRVYQPDTPPGEKAGEGFAPPAEAVASMMKYNEELA
                     KAGALIALDGLHPSSKGARVAFSGGKPKVTDGPFTEAKEVVGGYWMIEAKSKEEAVGW
                     ARRCPAADGDVIEVRQVFEMSDFPLDVREAADNPTVQAEIEKRKAS"
     gene            complement(1570..2190)
                     /locus_tag="VFK23_04870"
     CDS             complement(1570..2190)
                     /locus_tag="VFK23_04870"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET7318451.1"
                     /translation="MRPEGIPRCGWVGGEPLFRTYHDREWGVPVHDDRLLFEFLVLEG
                     AQAGLSWITILKKRDAYRAAFGGFDPKVVAGFDVGKVSELLANPGIVRNRLKVESAIT
                     NARSFLKVQEEFGSFDAYQWRFVDGRPIRNAWRSMKEIPASTPVSDAMSRDLKRRGFR
                     FVGSTICYAHMQAVGMVNDHTVDCFRWREVGRKPGIHRRIEHITKP"
     gene            complement(2187..>2469)
                     /locus_tag="VFK23_04875"
     CDS             complement(2187..>2469)
                     /locus_tag="VFK23_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318452.1"
                     /translation="DKSVQRMLNFKGWAPEEIRSINAPTLVMIGDHDIVRPEHAVLMF
                     RLLPNAQLAVLPGTDHMTIVNRTDWLVPMIETFLDSPMPPARSNGNVKR"
BASE COUNT          550 a          671 c          729 g          519 t
ORIGIN      
        1 gtgatggcgc agagcgcggt aaaggatgag tgcgttgcct tgtgcaagga cgcagcgaaa
       61 ttcatcaacg agaagggagt cgatgcggcc atcgcggaga ttggcaacaa agaaggcaag
      121 ttcgtaacga aaaacaccta cgtatttctc atggacctcg aagggaacag gcttgcccat
      181 ccctatgcga ggcctgaaga tccgaagatc atgaaattat tcgatcagaa agatacgacc
      241 gggaagctgt tcgttcagga gtacatcacg gttgccaaat cgaagggcga ggggtggacc
      301 gaatacacgt acccgactcc agaggaattg aaaaagccca ccccgtacaa ggaaaagaaa
      361 tcctcgaaaa aaatcagcta cgtctatcgc gtccctggca aggacctcat ggtgatcgca
      421 ggcttcttcg aataggtgcg gggatttctt ccgtgggagc gggcaaaata gagacccgtt
      481 tcagtctaag gggattctgc gacgaatacg ctgatcgagg aggtaaaaat gaaaagatgg
      541 ggattgttct tggcgatgtt gctctccgtt acggttcttc ttgcacctgc gacccaagcg
      601 gcggacgagg tcgcagggcg ggttgtctac catacgcaaa aggttgagat gatggaagcg
      661 ggcgacgttc caggtcatat tgtgggagtc ctccaacagt ccgggttgac tttttacaca
      721 aaggggcctg caagtgggca aatcgcaaca aggatgatga atacatccta tgacgttgtg
      781 aaatggatag ggagctacac agcatatata gtggattcat ttcaagacgg ttcgaccctg
      841 atttataagg ccagtggcac tattacaccg atagacggag ggaatcggac agcatttgaa
      901 ggcacatacg agattaccgg tggagcggga aggtttgaag ggaagaaagg gaaaggctcc
      961 tttaagggag agcgcattgg ttcgccaaaa acaggtggcg attcgtacgc ggacttcacc
     1021 ggcaccgaat ggaagtagca gtaactctgt agccatagtt atggaggacg gagcttaacg
     1081 gctccgtcct ctgaccattg atgtgcggcg tcaagatgct ttgcgcttct cgatctccgc
     1141 ctgcaccgtc gggttgtccg ccgcttcccg gacgtcgaga gggaagtccg acatttcgaa
     1201 cacttgacgg acctcgatga catcgccgtc cgcggcgggg cagcgcctgg cccatccgac
     1261 cgcctcctcc ttcgatttcg cctcgatcat ccaatagcct ccgaccactt ccttcgcctc
     1321 ggtgaacggc ccgtcggtca ccttgggctt cccgccggaa aacgcgaccc gggcgccctt
     1381 cgaacttggg tgaagcccgt cgagcgcgat gagcgcaccg gctttcgcaa gttcctcgtt
     1441 gtacttcatc atgctggcga cggcttcggc gggaggggcg aacccttccc ccgccttctc
     1501 ccccggaggg gtatcgggtt gataaacccg cgggatcatc atcatcatga atcgcattgt
     1561 cattcctcct tatggtttgg tgatgtgctc gatgcgtcga tgaattcccg gtttccggcc
     1621 tacctcccgc catcggaaac aatccaccgt gtgatcattc accatgccga ccgcctgcat
     1681 gtgggcgtag cagatggtgc tgccgacgaa gcggaaaccg cgccgtttca ggtcgcgact
     1741 catggcgtcc gatacgggtg tgctggcagg aatttccttc atgcttcgcc aggcgttccg
     1801 gatcggcctg ccgtccacaa agcgccactg gtaggcgtcg aaggagccga actcctcctg
     1861 caccttcagg aaggagcggg cattggttat tgcggattcc accttcaggc gattgcgtac
     1921 gatgccgggg ttggcaagta gttccgatac tttccctaca tcgaaaccgg ccactacttt
     1981 ggggtcgaag ccgccgaagg cggcgcggta ggcatcccgt ttcttcagga tcgtgatcca
     2041 ggagagcccg gcctgggctc cctccaggac caggaactcg aacaagagcc ggtcgtcgtg
     2101 gaccggtacc ccccattccc tgtcgtgata agtccgaaaa agcggttcgc cgccgaccca
     2161 gccgcagcgg gggatgcctt cgggtctcat cgctttacat tcccgttcga tcgagctggc
     2221 ggcatgggcg aatcgagaaa cgtctcgatc atcgggacca gccaatcggt gcggtttaca
     2281 atcgtcatgt gatccgtccc aggtagtacg gcgagctgag cgttcgggag caggcgaaac
     2341 atcaggacgg catgttcggg acggacgata tcgtggtcac cgatcatgac cagtgtcggg
     2401 gcgttgatcg accggatctc ttccggcgcc cagcccttga aattcagcat ccgttgcacg
     2461 ctcttgtcg
//
LOCUS       DASSLD010000270         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1026939, whole genome shotgun sequence.
ACCESSION   DASSLD010000270 DASSLD010000000
VERSION     DASSLD010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026939"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VFK23_04880"
     CDS             <1..504
                     /locus_tag="VFK23_04880"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HET7318453.1"
                     /translation="FYIKAHVPVPPVSFYEEFPQIENGVGMVADFLGRAGRTRKPAAV
                     PPIRATLVTGVSFSRILKDTAVGLSGIRGLSLRLLTVRNRFFGDSVTVAGLLTGSDIA
                     AALEGRRLGDLVVIPSEALKEDDGMFLDGMSLKQLSCRFGVTMAPAGSFRNLVNLLKK
                     GREGLTA"
     gene            501..1358
                     /locus_tag="VFK23_04885"
     CDS             501..1358
                     /locus_tag="VFK23_04885"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET7318454.1"
                     /translation="MISGKTKIYGIFGFPVEHTFSPGMHNAAFRKLGMDACYVPFAVN
                     PSRLKDAVKAVIPLGLGGLNITVPHKEKVIAWLDQLSEEARLIGAVNTIEVKEGRLIG
                     HNTDGRGFIRSLRDGAGFDPKGKSVLIAGAGGAARAVGFCLALAKAEKIAVYDVDAGK
                     AARLARDIRRKTGIAAEALSSRMLAAASNEAHCVINATPLGLKKNDPLPLGRELIRPH
                     HLVCDLVYNPPETGLLKAAKDRGARRLPGIGMLLYQGVIAFEQWTGARAPVPVMKYAL
                     ARQIAGRAR"
     gene            1443..>2194
                     /locus_tag="VFK23_04890"
     CDS             1443..>2194
                     /locus_tag="VFK23_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HET7318455.1"
                     /translation="MGMSGRLGSMLVSSGLITDDQLKKALSVQKTEGGRLGSILVKLG
                     SVQEDKLMTFLSKQYGVPYVDLNKFEINAVVIKHIPAEVAQKYRIMPINRTGATITIA
                     MVDPSNIFAIDDIKFMTGYNVDAVVATEGAILEAIKKYYGGAKSLLASKSGAAAAAQK
                     EEKKLTIEAKDYSLDDTQNEDSIIDDIDVGGVVDVDDFDDLVHGAVDDLEVVEDQALD
                     QLSGEVEAPIVKLVNGVLLRAAKLGVSDIHIEP"
BASE COUNT          455 a          652 c          677 g          410 t
ORIGIN      
        1 ttttacatca aggctcacgt gcccgttccg cccgtgtcct tctatgaaga atttccccag
       61 atcgaaaacg gcgtcggcat ggtcgccgac ttcctgggac gcgcgggacg cactcgcaag
      121 ccggcagcgg tccccccgat ccgcgcgacc ctggtgaccg gcgtttcctt cagcaggatt
      181 ctgaaggaca cggcggtggg cctcagcggg atacgcggcc tgtcgctgcg cctgctcacc
      241 gtccgcaacc gtttttttgg agactcggta accgttgccg gcctcctgac cggaagcgat
      301 atcgcagcgg ccctggaggg cagacggctg ggagatctcg tggtcattcc ctccgaggca
      361 ctcaaggaag acgacggcat gttcctcgac ggaatgagcc tcaagcaact aagctgccgt
      421 ttcggcgtga cgatggcgcc cgcgggaagc ttccggaatc tggttaacct gctcaaaaag
      481 ggaagggagg gactgacagc gtgatttccg gcaagaccaa gatatacggg atcttcgggt
      541 tccccgtgga gcataccttt tctcccggca tgcataacgc ggccttcagg aagctcggca
      601 tggatgcctg ttatgtccct ttcgcggtga atccatcccg gttgaaggac gcggtgaaag
      661 cagtgatccc cctggggctc ggcggattga acatcacggt gccgcacaag gagaaggtca
      721 tcgcctggct ggaccagctc tcggaggagg cgcgcctgat cggggcggta aacaccattg
      781 aagtgaagga ggggcgcctg atcggtcata atacggacgg gcgcgggttc atccgctcgc
      841 tccgcgacgg ggccgggttc gacccgaaag gcaagtcagt tctcatcgca ggcgcaggcg
      901 gcgccgcccg ggccgtgggt ttctgcctcg ccctggcgaa agcggagaag atcgccgtct
      961 acgatgtcga tgccgggaag gccgccaggc tcgcgcgcga tatccggagg aagaccggga
     1021 ttgcagccga ggcgctgtcc tcccgcatgc tcgccgcggc ctcgaacgag gctcactgcg
     1081 tgatcaacgc aacgccgctc gggttgaaaa agaacgaccc gctcccgctc ggcagggaat
     1141 tgattcgtcc gcaccatctg gtgtgcgatc tcgtgtacaa tccgcccgag accggcctgc
     1201 tgaaggcggc caaggaccgg ggcgcccgga ggctcccggg catcggaatg ctcctgtacc
     1261 agggggtcat tgcattcgaa caatggacgg gcgcacgggc ccccgttccg gtcatgaaat
     1321 acgctttggc gcggcagatc gccggtcgcg cccgatgacc ggtagagaca gtcatatctt
     1381 gacataccgt gaacattaaa gtaatattga gggtgatttc ttccggaatt taaggagttc
     1441 ccatgggaat gtcaggaagg ctcggctcca tgcttgtatc gtcgggcctc ataacggatg
     1501 accagcttaa gaaggcgctc tccgttcaaa aaacggaggg cggccggctg ggttccatcc
     1561 tggtcaagct ggggtctgtg caggaagaca agctgatgac ctttctcagc aagcagtacg
     1621 gcgtgcccta cgtcgacctg aacaaattcg agatcaatgc cgttgtcatc aagcacatcc
     1681 ccgctgaggt agcccagaaa taccgcatca tgccgatcaa ccggaccggc gccaccatca
     1741 ccatcgctat ggtggacccc tcgaacatct tcgccatcga tgacattaag ttcatgaccg
     1801 ggtacaacgt tgatgccgtc gtggcgaccg agggggcgat cctcgaagcg atcaagaagt
     1861 attacggcgg tgcaaagtct ctcctggcct ccaaatcggg cgccgcggcg gccgctcaaa
     1921 aagaagagaa gaagcttacc atcgaagcca aggattattc tctcgacgat acgcagaacg
     1981 aagactcgat tatcgacgat atcgacgtcg gcggcgtcgt cgacgtcgac gatttcgacg
     2041 acctcgtcca cggagcggtc gatgacctcg aagtcgtcga agaccaggcc ctggaccagc
     2101 tgtccggcga agttgaggcg cccatcgtca agctggtgaa cggtgtcctt ttacgggcgg
     2161 ccaagctggg cgtctccgac atccatatcg agcc
//
LOCUS       DASSLD010000271         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_453165,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000271 DASSLD010000000
VERSION     DASSLD010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453165"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2186)
                     /locus_tag="VFK23_04895"
     CDS             complement(<1..2186)
                     /locus_tag="VFK23_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7318456.1"
                     /translation="MLEKALKESRKFLETIIETEPECVKLVASDGTLIMMNRAGLDMI
                     QVGSLDRAKGRSIYPLILPEYREAFVKLTEEVFQGRPGTLTFEMKGVKGRRLWLETHA
                     VPLRNESNEIIALLGITRDVTGRKRDEATILEQKRFAENLVTNSAVATFVLSAQHRVL
                     FWNKACEELTGKSAADMVGSDDHWKAFYDSRHPTLADILIDGETEQAASLYSVFAPSV
                     LIPSGLRAEGWFPRVNNRDRYLAFDAAPVYDGKGALSVVIETIHDVTEHRKAEEALMQ
                     EKKFSDAVIDNLPGIFYICDERGSLIRWNKNVQEVTGYTGEELSRITMSGLFLEDREL
                     VARKMKEVYDAGKAFAEARVLTKTGERIPFLLTAFRLLIHDERYLVCVGIDVSERKRL
                     EDQLRHAQKMESIGTLAGGIAHDFNNILTAVIGYGNLLQKKIGEDQSLRFYTEQILTA
                     ANRAAGLTRGLLAYGRKQILNPQPVNLNEIIRNMENLLARLIGEDVELTSILSEKDLI
                     VLADTGQIEQVLMNLATNARDAMPGGGSLYLDTGAIELGEDEAKAHGLQRAGAYALLT
                     VSDTGTGMDEKTRERIFEPFYTTKEVGKGTGLGLAMVYGTIRQHNGSVEVESDPGKGA
                     AFKIYLPLVQVASDASQPGVLPPIAGGTETILVAEDDEAVRNLTAGVLTQAGYTVIQA
                     ENGEAAVNKFSENRAAVRMLILDVIMPRKNGKEVYDKIRIFKPDIKA"
BASE COUNT          490 a          776 c          702 g          631 t
ORIGIN      
        1 gccttgatgt cgggtttgaa aatcctgatt ttatcgtaca cttctttgcc gttcttcctc
       61 ggcataatga catccaggat aagcatgcga accgcggccc ggttctcact gaatttgttg
      121 accgccgcct ctccgttctc agcctggatc accgtgtacc cggcctgcgt caacacgccg
      181 gcagtaaggt ttctcacggc ctcatcgtcc tcggcaacaa gtatggtctc tgtgccccct
      241 gcaatgggcg gaagaacgcc gggttgcgag gcgtccgacg cgacctgcac taacggcaga
      301 taaatcttga acgccgcccc cttccccggg tcgctttcga cctccacgga cccattgtgc
      361 tgccgaatgg tgccgtatac catggccagc cccagtcccg tgccctttcc cacctctttt
      421 gtggtataga agggctcgaa gatccgctca cgggtctttt catccatgcc ggtgcccgtg
      481 tcggagaccg taaggagggc atacgcgccg gccctttgga gaccgtgcgc cttcgcctcg
      541 tcctcgccca gttcgatggc ccccgtgtct aggtagaggc tcccgccgcc gggcattgca
      601 tcgcgagcgt tcgtggccag gttcatcagg acctgctcga tctgccccgt gtccgccagg
      661 acgatcaagt ccttctcgct caggatgctc gtgagttcca cgtcttcccc gatgagcctc
      721 gcgagaagat tctccatatt ccggataatt tcgttgaggt tcaccggctg cgggttcagg
      781 atctgcttcc tgccgtaggc aagcaatccg cgggtcaggc cggcggcccg gtttgccgcg
      841 gtaagtatct gttccgtata gaaccggagc gattggtcct cgcctatctt cttttgaagc
      901 aggttcccgt agccgatgac ggctgtaaga atattattga agtcgtgagc gatcccgccg
      961 gcgagggtgc cgatggattc catcttctgg gcgtggcgaa gctggtcctc gagccgtttg
     1021 cgctccgaaa cgtcgatgcc cacgcacaca agataccgct cgtcgtggat aagaagccgg
     1081 aatgccgtca acagaaaagg tatgcgctcg ccggtcttcg tcaggaccct ggcctccgca
     1141 aacgccttgc cggcgtcata gacctccttc attttacgcg ccacaagctc cctgtcctcg
     1201 aggaaaagac cggacatggt tatgcgcgac aactcttcgc cggtataacc cgtcacctcc
     1261 tgtacgttct tgttccaccg gatcagcgac ccgcgctcgt cacagatgta aaatatcccc
     1321 ggcaggttgt cgatgacggc atcggagaat tttttctcct gcatcagggc ttcttcggcc
     1381 ttgcggtgct cggtgacgtc atggatggtt tcgatgacga ccgacagcgc tcctttgccg
     1441 tcatagacgg gggccgcgtc aaaggcgagg tagcgatctc tgttgttcac ccgcggaaac
     1501 cagccttccg cacgcaggcc gctgggaata agaacggagg gggcgaagac ggaatagagc
     1561 gaagcagcct gctccgtctc gccgtcgatg aggatgtccg caagcgtagg gtgcctgctg
     1621 tcgtaaaacg ccttccagtg gtcgtcgctg ccgaccatgt ccgcggcaga tttgccggtc
     1681 agctcctcgc aagccttgtt ccaaaacagg acgcggtgct gggcgctcag cacaaaggtc
     1741 gccaccgcgg agttggtcac caggttctca gcaaagcgtt tctgctccag gatcgtggct
     1801 tcatctcttt tgcggcccgt aacatcgcgc gtgatcccca ggagggctat gatctcattg
     1861 ctttcgttgc ggaggggaac agcatgggtc tcgagccaga gccgccttcc cttcacgccc
     1921 ttcatctcga aggtcagggt ccccggcctg ccctgaaaaa cctcttccgt aagctttacg
     1981 aaggcctccc ggtattccgg caggataaga ggataaatcg atctgccctt ggcccggtcg
     2041 agcgaaccca cctggatcat gtccaggccg gcgcggttca tcatgatgag ggtgccgtcg
     2101 gatgcgacaa gcttgacgca ttccggctcg gtttcgataa ttgtctcgag aaatttcctg
     2161 ctttctttga gtgctttctc cagcacgccg ctgccggagc ccgggttagc ggaagcggtg
     2221 ccgcgtatta tcttttcctg agtcatatga tcgtatgttc tgcggcctta aaccgctcgt
     2281 tcgtaaaaac actcatctca atcctatgct tctctttagt atagcaaatg cccgggtctg
     2341 cctcgggcgc agcgggatat gtttttttaa gtcaataatc ctaaaaaagc ggttggacgc
     2401 cgataaacgc tgattttctc ggattaaatc gagagacacg tatgtttgga actttatcca
     2461 aatggcgagc ctttaataaa gaagaaatgg catttattta tagaccatat ccgctgtaga
     2521 cttcatccgc gtgtatccga tcatatttgc gtcccaattg ccgtttttct tcggcccttc
     2581 tttcacctgg tccgttttt
//
LOCUS       DASSLD010000272         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_430325,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000272 DASSLD010000000
VERSION     DASSLD010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430325"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1268)
                     /locus_tag="VFK23_04900"
     CDS             complement(108..1268)
                     /locus_tag="VFK23_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HET7318457.1"
                     /translation="MYEQLTSIITQILSHTVHVFANKLVPLAEAGGSTEATGLVPVST
                     VYVTLIVLAGLGIVFGVALAIIAARFVVKVDPKVEQVRETLPGANCGACGFAGCMGYA
                     EAVVGNPDVAASMCAPGKSPVAEKIAVITGKKAEKMEPKIARVFCQGGTSLSQRKFIY
                     TGVQDCTAAVLAAGGDKSCQYGCLGYGTCMRACPFDAITMSADNLPQINPDKCTACGK
                     CVAACPKQVIELAQASKAVVISCHSRDKGVDVKKKCQVGCIACGICVRTCPVNAIAIE
                     NNLARIDHSKCIVCGLCVKKCPTSAIFDYIPVRPKAFIDPAKCAGIDMCEKVCPVNAI
                     SGDVRQVHVVNQEKCIGCRMCEPRCPKKAITMVEASAAAAKKPAKAQEPVAV"
     gene            complement(1358..1942)
                     /locus_tag="VFK23_04905"
     CDS             complement(1358..1942)
                     /locus_tag="VFK23_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HET7318458.1"
                     /translation="MLQHVLTIFIASALINNFVFSRYLGLCIFFGVTKKMETSIGMSF
                     TFTTVMLISASLNWLIYNYVMVPYHLGFLKIVIFIGVIAAFVQAADTIMKKVAPALYY
                     KLGIYLALIVTNCVILAVPLLNADENYSLFESMAMAVGSGLGFSLALIIMASIREKLE
                     LADVPKSFVGLPISFVLTGLIALAFLGFSGMITL"
BASE COUNT          456 a          638 c          619 g          456 t
ORIGIN      
        1 aaggaatcag cccgcaccgt gtcggtggtt tttttacggg gcgaataaaa aaggggatgc
       61 attacgggca tcccctttct catgacatcg tgcgttcgtg cagaccgtta gacggcgacg
      121 ggctcctgcg ccttcgcggg cttcttcgcc gcggcggcgg aggcctctac catggtgatc
      181 gctttcttgg ggcaccgggg ctcgcacatc ctgcagccga tgcacttctc ctggttgacg
      241 acatggacct ggcggacatc tccggagatg gcgttcaccg ggcagacctt ctcgcacatg
      301 tcgatgcccg cgcacttggc gggatcgatg aaggccttcg gcctgaccgg gatatagtcg
      361 aagatcgcgc tggtgggaca tttcttcacg cagagtccgc agacgatgca cttgctgtgg
      421 tcgatccgcg cgaggttgtt ctctatggca atggcgttca ccgggcacgt ccttacgcat
      481 atgccgcatg cgatgcagcc cacctgacac ttcttcttta cgtcgacacc cttgtccctg
      541 ctgtggcagc tgatcacgac cgccttggac gcctgggcga gttcgatcac ctgtttcgga
      601 caggcggcca cgcactttcc gcaggcggtg cacttgtcag ggttgatctg cggcaggttg
      661 tcggcgctca tggtaatcgc gtcgaagggg caggcgcgca tgcaggtgcc gtagccgagg
      721 cacccgtatt ggcaggactt gtccccgccg gcggcaagta ccgccgcggt gcagtcctgc
      781 acgccggtgt aaatgaactt ccgctgggag agggaggtgc ccccctggca gaagacacgc
      841 gcgatcttgg gctccatctt ctcggccttc ttgcccgtga tcacggcgat cttctccgcg
      901 accggggatt tacccggcgc gcacatgctg gccgcaacgt cggggttgcc tacgactgcc
      961 tcggcatagc ccatgcagcc cgcgaatccg caggcgccgc aattggcgcc gggcagtgtc
     1021 tcgcggacct gctccacttt cgggtcgacc ttcacgacga accgtgcggc gatgatggcc
     1081 aaagccacgc cgaaaacaat acccagtccc gcaagaacaa tcaacgtaac atagacggtc
     1141 gaaacgggca cgagcccggt cgcctccgtc gagccgccgg cctcggcgag ggggaccagc
     1201 ttgttggcga agacatgcac ggtgtgcgag agaatttgtg taatgatact cgtcaattgt
     1261 tcatacatgg gtttgtacct tcagcttagt tattagaagc cacggacccc gggtgcggtt
     1321 ttttaatccg cattccgcaa tccgcaatca gcattcgtta cagcgttatc atccccgaga
     1381 atcccaggaa cgccagggcg atcaagccgg tcaacacaaa ggatatcggc agccccacaa
     1441 atgacttggg cacatctgca agttccagct tctcccgtat gctcgccatg atgatgaggg
     1501 ccagactgaa ccccaggccg gagcccactg ccatggccat gctctcgaaa aggctgtaat
     1561 tttcatcggc gttcagaagc ggcacggcca ggatgacaca gttcgtcacg atcagcgcaa
     1621 ggtagatgcc cagcttgtag tacaatgccg gggcgacttt cttcataatc gtgtccgcag
     1681 cctgcacgaa ggcggcgatc acgccgataa aaataacaat cttcaggaac ccgaggtgat
     1741 agggaaccat gacatagtta taaataagcc agttcaggga cgcggaaata agcatgacgg
     1801 tcgtaaaggt aaagctcatt ccgatcgatg tttccatctt tttcgtcacg ccgaaaaaga
     1861 tgcagaggcc gagatagcgg gaaaacacga agttgttaat gagcgccgat gcgatgaata
     1921 tggtcagaac atgctgcaac ataggaaccc tccgggtgaa acgatttcgg atttcagaca
     1981 gtgtggcccg tttgcttggc cattgcgtat ttcggatttt aattccgcat tccgcacttc
     2041 ctaatgtcct ccgctgctcg cacccttgcc gccgaagaac ttccggtcga tccagttgag
     2101 ccagcccatc acgagaccca tggcaaagaa cccgcctgtc ggcatgatca tgatgaacac
     2161 cggtttgcc
//
LOCUS       DASSLD010000273         6516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_825184,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000273 DASSLD010000000
VERSION     DASSLD010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825184"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VFK23_04910"
     CDS             complement(<1..768)
                     /locus_tag="VFK23_04910"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET7318459.1"
                     /translation="MTSRRTDWTGTFVPVVTPFTRTGELDEPSIRRVLDLLIEEGVDG
                     IIVAASTGEWFSLSNEERVRLFAIAFEQVNKRVRLLAGTAAMATRDAVFLTDAARKLG
                     LDGALILPPPYVLPSERETVAFFAAVAEVGLPLMLYNNPSRTQVNINTRLLGKLVGFD
                     AVVALKDSVKDLGQLGETLRAWKDELAIFTGMEPYAAPCLSRGATGVVAMTPNVMGRE
                     AVQLPVLVAKDRGAELLPLQEKIDRFYERMYQWGYNPY"
     gene            1880..2851
                     /locus_tag="VFK23_04915"
     CDS             1880..2851
                     /locus_tag="VFK23_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HET7318460.1"
                     /translation="MGAITDLPDGPIHILVSSCLLGEKVRYDGGHKRDPFLVETLGRF
                     VTYVPICPEVECGLPTPREAMRLQGDPADPRLVTSRTGVDHTGKMQSWIRRKMLDLEG
                     LDLCGYICKKDSPSSGMERVKIYGDTGIPAKTGAGMFTKAFMDRFPLIPVEEEGRLQD
                     PVLREMFVEMVFTLRRLRDRIRQGKTRRNLVEFHTDHKLLILAHGRTRYDEMGKLVAH
                     AKELSLEELYRRYLQLLMEALRLRTSSAKCADVLMHMMGHLRRFLTPDEKQELLEVIG
                     RQRNRLIPLLVPVTLLRHYVRKYDVAYLKRQVFLNPHPVELMLRNHV"
     gene            2844..3869
                     /locus_tag="VFK23_04920"
     CDS             2844..3869
                     /locus_tag="VFK23_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET7318461.1"
                     /translation="MFDIGMKPVLVIGATGYVGARLVARLVKRGVPVRAAGRSRQKLL
                     VRPFSHDPLAEVATCDVLDLPSLREACAGCSVVYYLVHSMDPGQQEFSDADREGAQNM
                     VRAAELEGVKRIIYLGGLGDPGANISKHLRSRIEVGEILVGGKVPVTILRAAMIIGGG
                     SGSYEILRYLVDRLPVMITPRWVSTESQPIAIRNVLDYLIGVLEVSTTTGGTFDIGGP
                     EVVSYRRLMDLYAEEAGLRKRWIIPVPVLTPRLSSYWIDLVTPAPAYLARPLAEGLSS
                     RLVCGDDRIRRLVTVELLDCRSAIRAAIPGRRRHTAGTGGAEGTEGQDEAKIIPGDPE
                     WVGKVLA"
     gene            4156..4719
                     /locus_tag="VFK23_04925"
     CDS             4156..4719
                     /locus_tag="VFK23_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HET7318462.1"
                     /translation="MGERRLARLNEEFDVRVKRRFLEIHPETPAEGTPISELGYPPDQ
                     WTRMMENLERMGKAEGIGFSERTFTTNSHKALLLAEAAKEEGPEVFEALNEGLFRAYF
                     TEGRNIGDPQVLRDVAQAAGVPDRRLEQAWSDSAYEERLARDHAAATEIGITGIPVFI
                     IDGRWILEGAVPVEMLREAAKKILGKS"
     gene            complement(4808..5227)
                     /locus_tag="VFK23_04930"
     CDS             complement(4808..5227)
                     /locus_tag="VFK23_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET7318463.1"
                     /translation="MKSYRKELWFDIPGRRAFVNITPQVEASLRESGIKEGLLLCNAM
                     HITASVFINDDESGLHKDYDRWLERLAPHEPVSQYFHNRTGEDNGDAHLKRQVMGREV
                     VVAVTGGELDFGPWEQIFYGEFDGGRRKRVLVKVIGE"
     gene            5300..5893
                     /locus_tag="VFK23_04935"
     CDS             5300..5893
                     /locus_tag="VFK23_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET7318464.1"
                     /translation="MAHKPTRAEAWTILTDHVKSPSLLSHALAVEAVMRHVARKKFAD
                     EELWGVIGLVHDVDYEEHPTEHLRHAPEILRSQGWPEECIRAVASHGWGLCTDLEPVT
                     DMEKTLFAIDELTGLVAAAALVRPSRSVLDLPVKSVLKKWKDKTFAAGVDRKVIEKGA
                     ALMGVPLEELIADTIEGMKTVAETIGLKGNPGGGSIP"
     gene            complement(5982..6215)
                     /locus_tag="VFK23_04940"
     CDS             complement(5982..6215)
                     /locus_tag="VFK23_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318465.1"
                     /translation="MKVRVVCYEGYKAEETPRAFLLGERRVEVVRILDRWRGEDHEYV
                     KLDGSDGARYILRYDRSRDEWEIQMMESPPSTA"
BASE COUNT         1372 a         1944 c         1864 g         1336 t
ORIGIN      
        1 gtaaggattg tatccccact gatacatcct ctcataaaac cggtctatct tttcctggag
       61 tggaagcaat tccgcccctc tatccttagc aacaagcaca gggagctgca ccgcctcccg
      121 ccccatgacg ttgggcgtca ttgctaccac accggttgcg cctctcgaga ggcacggcgc
      181 agcgtaaggc tccataccag taaagatggc cagctcatct ttccaggccc ggagcgtctc
      241 cccaagctgc cccaaatcct tcacgctgtc cttgagcgcc accacagcgt caaaacccac
      301 aagcttccca agaaggcgcg tgttgatgtt gacctgcgta cgcgatggat tgttgtaaag
      361 catcagtgga aggcccacct ctgcaaccgc cgcgaagaac gccaccgtct ctcgctcgga
      421 tggtaggacg tacggagggg gcaggatcaa cgccccgtca agccctagtt tccgggctgc
      481 atccgtcaag aatacagcat cccgggtcgc catcgccgca gttccagcca gcaatcgaac
      541 cctcttattg acctgctcaa atgcaatcgc aaacaaacgg acgcgctcct cgttggatag
      601 ggaaaaccac tcgcccgtgc tggcggcgac gatgatcccg tccaccccct cctcgatgag
      661 caagtcaagg acgcgtctaa tggacggctc atccaactcg ccggtccggg tgaacggcgt
      721 cacgacaggc acaaaggttc cagtccagtc tgttcttcta ctcgtcatta ttgctcttac
      781 acctttgtct ggatttttca ggcaggcttt gtcaaattcc gacagcgcct ccttcctgta
      841 tcataaccga acaaggaaat attctgttcc cccagccgaa tttttacaat gtcatcgtca
      901 gccatatatg catgcaagac gtttgtatga atttttcgcg actcattgat aaagtcctcc
      961 agatcagatt tccgatgggt tttacacgta atcaatgatc gcgtggtctg atcgccgtca
     1021 ttaggccaac tgtatgaagg gatttcgcac aaaacgaaac cagaaatccg gtcacaagaa
     1081 ggtcactggc cgatatttgg gatcgaatat tatttcaacg gaggccactt ggagggatcg
     1141 accttcgggg tgggtgtgaa atccagtaac gctcaggctg agctgcgggc actcacgacg
     1201 ttcgccaggt gagccgtcag cttcagcctg tggttaggcg ccattctttt tattgagtcg
     1261 aataatatcc gtgtgaattt tatccaattt atttaatata tcctttgatg taacactgtt
     1321 cttatatgca ttgataataa gtttaacgga aacaagaaaa acacccgttt caagaagcaa
     1381 atcgtgtgtg aaacctttat agaaaagagc tgcaataaac agtattagcg taataaatat
     1441 aacggttagt gatccaaaat cgaaatgttt cattgtttaa atacttcctc cattttgaat
     1501 gtgcgcctaa cagttgatta gacggcagca ttaataacag tgcatgtaca gcaaccgtat
     1561 agcacccttg ttaggcggtg ggtgtatagc ttgactgaga gccagaccct tgcaggcagg
     1621 tccgtttcca cgagtatata cactaaccaa tttattttca gtcacaagcc gggcacaaac
     1681 cgtagggtac ggtctgatac gctttctagc atggcaaatt tttaggatcg accttcgggg
     1741 tgcgcatctt acggccacaa ggagaccgga aacttgcctc ccctgttgcc atccaaatgt
     1801 ataacttccg cgtgaggagg cagaccggtg atcttccgtt tgttgatccg tatcggtccc
     1861 tccggcaagg tatagcattt tgggcgccat aaccgatttg ccggacggtc ctatccatat
     1921 cctcgtcagc tcctgcctgc tgggagaaaa ggtccgctac gacggcggac acaagcgcga
     1981 tccttttctc gtggagacct tgggacggtt cgtcacgtat gtcccgatct gcccggaggt
     2041 ggaatgcggg cttccgactc cgcgcgaggc gatgcgcctt cagggagatc ccgccgatcc
     2101 gcgactggtc acctcaagaa caggtgtgga tcacacagga aaaatgcaga gctggattcg
     2161 caggaagatg ctggatctcg agggcctgga tctttgcggc tatatctgca agaaagattc
     2221 gccgagttcg gggatggagc gcgtaaaaat ctacggcgat accgggatcc cggcgaagac
     2281 cggagctggg atgttcacga aggccttcat ggatcgtttt ccgctgatcc ccgtggagga
     2341 ggagggccgg ctacaggatc cggtcctgcg ggaaatgttc gtcgagatgg tgttcaccct
     2401 gcgccggctg cgcgaccgga ttcggcaggg gaagacccgc cggaacctcg tggagttcca
     2461 cactgaccac aaactcctga tccttgccca cggcaggacc cgttacgacg aaatggggaa
     2521 gctggtggcc catgcgaagg aactatccct cgaggaactt tacaggcgct accttcaact
     2581 cctgatggag gcgcttcggc tgcgcacctc ttccgccaaa tgcgccgacg tgctgatgca
     2641 catgatgggg catcttcggc ggttcctgac tccggatgag aagcaggaac ttctcgaggt
     2701 gatcggccgg cagagaaatc gcctgatccc gctgctcgtg cccgtaacgc tgcttcggca
     2761 ctacgtccgc aaatacgatg tcgcctacct taagaggcag gttttcctca atccccaccc
     2821 tgtcgagctg atgcttcgga accatgtttg acatcgggat gaaacccgtt ctcgtcatcg
     2881 gagcaaccgg ctacgtggga gcccggctcg tcgcgcgcct ggtgaaacgc ggggttccgg
     2941 ttcgcgccgc gggtcgaagc cggcaaaagc tcctcgtccg ccccttttcc catgaccccc
     3001 tcgccgaggt cgccacttgc gacgttctgg acctgccctc gctccgggag gcctgcgcgg
     3061 gttgttccgt cgtctactac ctggtccact cgatggatcc aggacaacag gagttctccg
     3121 atgcggatcg ggaaggggcg caaaacatgg tccgggcggc cgaactcgag ggcgtgaagc
     3181 ggatcatata cctgggggga ttgggggatc caggcgcgaa tatcagcaaa catctccggt
     3241 cccgcatcga agtgggagag attctcgtag gagggaaggt cccggtgacc atcctccggg
     3301 ccgcaatgat catcggcggg ggaagcggct cgtacgagat attgcgctat ctcgtcgacc
     3361 ggcttcccgt aatgatcacg ccccggtggg tctccacgga atcccagccg atcgcgatcc
     3421 ggaacgtcct cgactacctc atcggcgtcc tcgaagtctc cacgacaacg gggggaacct
     3481 tcgacatcgg gggccccgag gtggtttcct acaggcgtct catggacctc tacgcggagg
     3541 aggccggcct ccggaagcgg tggatcattc ccgtcccggt gttgaccccc cggttgagtt
     3601 cctactggat cgaccttgta acccccgctc ccgcgtacct cgccaggccg ttggcggaag
     3661 ggttgagcag ccggcttgtc tgcggcgatg accgtatccg ccggctcgtc accgtggagc
     3721 tcctggattg ccgcagcgcc atccgcgccg cgatcccggg caggcggcga catacagcgg
     3781 gtaccggcgg ggccgaggga accgaaggac aggatgaggc gaaaatcatt ccgggagacc
     3841 ccgaatgggt tggtaaagtc ctggcatgaa gcttgagaac atccataccc tgtgtcatct
     3901 ccccgttccc atttcgaagg cctggaattt cttcgccgac ctccggaacc tagaacggat
     3961 cactccgtcc ttcctctcga agatacgttc gggaggctga ctgtccctcg cggctgaaac
     4021 tttgccccgc cggagggctt cgaaccgttg ttggaaccga atcgctcaca aggaggttcg
     4081 ccgaccatgc ccgccgggaa aaaacccatg ctgccggtga cggtgttcac cgactacatc
     4141 tgaccgttct gctacgtggg cgagaggcgt ctcgcccgtc tcaacgagga attcgacgta
     4201 cgcgtgaagc ggcggtttct ggagatccac ccggagaccc ccgccgaagg tacgcccatt
     4261 tccgagttgg gctatcctcc cgatcagtgg acccggatga tggagaacct cgagcggatg
     4321 ggaaaagcgg aggggatcgg tttctcggaa cggacattca cgacgaactc ccacaaagcc
     4381 ctcctcctcg cggaggcggc gaaggaagag ggaccggagg tcttcgaggc gctcaacgag
     4441 ggtctgttcc gcgcctactt caccgagggg aggaatatcg gtgacccgca ggttctccgg
     4501 gacgtggcgc aggcggcggg cgtccccgat agacggttgg aacaggcgtg gtccgattcg
     4561 gcctacgagg aacggttggc ccgcgaccac gcggcagcaa cagagatcgg catcacgggg
     4621 atccccgttt tcatcatcga cggccggtgg atcctcgagg gcgccgtacc cgtggagatg
     4681 ctccgcgagg cggcgaaaaa gatcctaggg aagtcatgac cggtatctgc aagcggctgg
     4741 aattatttac gataaaccgt cgcaggccaa agccggcgcg gcaagcgcag tcgatttccc
     4801 cgacgcttca ctcgccgatg accttgacca gcacccgctt ccgccgtccc ccgtcgaact
     4861 cgccgtagaa gatctgctcc cacgggccga agtcgagttc gccgcccgtg accgccacca
     4921 ccacctcccg ccccatcacc tgccgcttga ggtgcgcgtc cccgttgtcc tccccggtcc
     4981 ggttgtggaa gtactgcgag accggctcgt ggggcgcgag ccgctccagc caacggtcat
     5041 agtccttgtg gaggccgctc tcgtcatcgt tgatgaagac ggaggcggtt atgtgcatgg
     5101 cgttgcacag cagaagcccc tccttgattc cgctttcccg cagggatgcc tcgacttggg
     5161 gagtgatgtt gacgaacgcg cgtcgtccgg gaatgtcgaa ccaaagctcc ttccggtagc
     5221 tcttcacgtt ccgcctcctc gccgtgcggg atgacgctat atcgcgcaga cgattgcatt
     5281 tcgataatat ggatacatta tggcccacaa acccacccgc gcagaagcgt ggacgatcct
     5341 cacggatcac gtgaaatccc ccagtctcct gtcgcacgca ctggcggtcg aggcggtcat
     5401 gcgccatgtc gcccggaaga aattcgccga cgaggaactg tggggcgtca tcgggctcgt
     5461 ccacgacgtg gactacgaag aacaccccac ggaacacctc cggcacgcgc cggagatcct
     5521 caggagccag gggtggcccg aggagtgcat acgggcggtc gcgtcccacg gttgggggct
     5581 atgcacggac ctcgaaccgg tcaccgacat ggagaagacg cttttcgcga tcgacgaact
     5641 gacgggactg gtcgccgcgg ctgcgctcgt ccggccgtcg cgaagcgtcc tggacctacc
     5701 cgtcaagagc gtcctgaaga agtggaagga caagacgttt gccgcgggtg tggaccgcaa
     5761 ggtgatcgag aaaggcgccg cactgatggg ggtaccgctc gaagaactga tcgcggacac
     5821 catcgagggg atgaagaccg tcgcggaaac gatcggcctc aagggaaacc cgggcggggg
     5881 atccattcct tgagacaccg cccaaacggc gacaggggac cttgttgtca gagtctttcc
     5941 cggtacactt ccttgcgatg gtcaaccttt actcctggtg atcaggcggt ggacggaggc
     6001 gattccatca tctggatctc ccactcgtcc cgggaacggt cataccggag gatgtagcgg
     6061 gcgccgtcgg agccgtcgag ctttacatac tcgtggtcct ctccccgcca tcggtccagg
     6121 atgcgtacca cctcgacacg gcgctccccg agcaggaagg ctcgaggcgt ctcctccgcc
     6181 ttgtaccctt cgtagcagac cacgcgaacc ttcattccct gctccaacaa cgaaatcggt
     6241 tatccagtca gaagacactc ccagatccga tttatggatc gtatcctctt tcaaacaaga
     6301 taacttgagg aaaccgacct tcgggttggc tgcatgaagg tccaagaata gcgggtcggg
     6361 tcggatgtcg cggggagtcc cgcgttccat catggatccg gatccgctgc gcatcccgca
     6421 tcgagttcgt atgtctccgg gcgtactacc ttaccgtcat cgatccagga gggcgcaccc
     6481 ccgccgacga tgcagaggac cctttctttc ctgagc
//
LOCUS       DASSLD010000274         3661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_685667,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000274 DASSLD010000000
VERSION     DASSLD010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685667"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1174)
                     /locus_tag="VFK23_04945"
     CDS             complement(107..1174)
                     /locus_tag="VFK23_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7318466.1"
                     /translation="MKKKKISLIAASAVIVFAIVVFVAYSRASNDRSETAVATAVVEK
                     RDMGSLVQATGIIKPKVGAEVKVGARITGKVEHLYANIGDRVKKGQVLVRLEQDDLKA
                     RADQAEAAYLEAAAAFDKAKIDLERDKPLAQQGYISQQNIDVLQNAYDMTKARLLKAK
                     ADQDYAKAQLSYATITAPISGTIASVTTQQGETVAAGLNAPTFITIIDLNKLEVNAYV
                     DETDVGKIGVGQEALFTVDTFADKDFKGRVTAIYPRAVLQENVVNYITLISIENSEGK
                     LKPDMTANVTITLKTKKGVLAIPGAAVTREGGKKYVMLQAKDGKAARREVKTGWKEGP
                     YLEITSGLTEGDVVVTNEGKK"
     gene            complement(1174..2547)
                     /locus_tag="VFK23_04950"
     CDS             complement(1174..2547)
                     /locus_tag="VFK23_04950"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7318467.1"
                     /translation="MAVITAFFSAPTGAAERRLPGSPSKQTAGAETVKADALPGNGTT
                     SPRLLTLDQCIEIALANNPGLQIEQEKIIELENDYSIASAGLYPKITASAFYTRVNRD
                     RVGIQPIMQYTEESLGQVKLKQLLYDGGKTRYSGRAASKAADAQRDSAEATRLDTVYA
                     VSQAYYRVLEARELVRVGENSRSQREAFFKLTDSYQKAGKATRIESLKAEAQLLDAER
                     SLVQAHEALRISELILKKTIGVGVEAQINIADGLPDSFAEPGNEEFLLAQMYENNPDL
                     KKSAQFKEQARLSIDSASGAYMPEISLQGNYGYRDRDVGGAGDEWLAGVFLEWPLFEG
                     GITRAQVGKARARYREIEWSDRAVHDQVQVDLSQALGDIRTALASIRSSKRLVEAQDE
                     AYKAVVEFYKRGKATYLEVLTADTDLTQAKAAYVLAVGDYQAASARLDRVTGKRYAAS
                     ERQGRLP"
     gene            complement(2976..3467)
                     /locus_tag="VFK23_04955"
     CDS             complement(2976..3467)
                     /locus_tag="VFK23_04955"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HET7318468.1"
                     /translation="MKSSAFVKFALAASLVLNLSFLTAAGYTYYQQSRTWTSPFGTKM
                     EKGRFLFEELSLKPEQLKALRERTLPFRAEIDKRSIEIGDKRKALIALLRQDNPDQKA
                     VNAAIAGINRMQEEMQERIAAHMLEVKASLDRDQQQKFFDLIENTMAQTGQVGCPPGD
                     LHH"
     gene            complement(3457..>3661)
                     /locus_tag="VFK23_04960"
     CDS             complement(3457..>3661)
                     /locus_tag="VFK23_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318469.1"
                     /translation="FAKFAEAAVLLFVIFIGIAAGSFIMKGLPSQNATSLASSMSLDL
                     FDAAPPGSLGGAYLAMTEANHEK"
BASE COUNT          682 a         1086 c          966 g          927 t
ORIGIN      
        1 gcccccgttc ttccgatatg tcttggttat gtccctcagt tcgatcatgt atctatcctc
       61 cgttttggct tgacgattct aattccgcat tccgaattcc gtaatgttat ttctttccct
      121 cattcgtcac caccacatcc ccttccgtga gacccgaagt tatctcaaga tacgggcctt
      181 ccttccatcc cgtcttgacc tcgcggcggg ccgccttgcc atccttcgcc tgaagcatta
      241 catatttctt tccgccctcg cgcgtgactg cggcgcccgg gatcgcgagc accccttttt
      301 tcgtcttgag cgtgatcgtc acgttcgcgg tcatgtcagg cttgagcttt ccctcggagt
      361 tctcgatgct gatgagtgtg atatagttga ccacgttctc ctgcaacacc gcccgtgggt
      421 agatcgcggt caccctgcct ttgaaatcct tgtcggcaaa ggtgtctacg gtaaaaagcg
      481 cttcctgacc gaccccgatc ttgcccacgt cggtctcgtc cacataggcg ttcacttcga
      541 gcttgttcaa atcgatgatt gtaatgaagg tcggcgcgtt cagcccggcg gcgacggtct
      601 ctccctgctg ggtggtcacc gacgcgatcg ttccgctgat cggtgcagtt atggtcgcgt
      661 acgagagctg cgccttggca tagtcctggt ccgccttggc tttgagcagc cgcgctttcg
      721 tcatgtcgta cgcgttctgc agaacgtcta tgttctgctg agaaatatat ccttgttgcg
      781 cgagcggctt atcccgttcc agatcgatct tcgccttgtc gaatgcagca gccgcctcca
      841 ggtaggccgc ttcggcttga tcggcccggg ccttgagatc gtcctgctcg agccggacaa
      901 ggacttggcc tttcttaacc cggtcgccga tgttggcgta gagatgctcg accttgccgg
      961 tgatccgcgc cccgaccttg acttcggccc cgaccttggg tttgatgatg cctgttgcct
     1021 ggaccaggga tcccatgtcc cgcttctcca cgaccgccgt cgcgacagcg gtctccgacc
     1081 tgtcattgct cgccctggaa taggcgacaa aaacgactat ggcgaacacg ataaccgcgg
     1141 atgccgctat gagactgatc ttcttctttt tcatcacggt aatcttccct gcctttcgct
     1201 tgctgcatag cgctttccgg ttacccggtc aagacgcgct gaagcagctt gataatcccc
     1261 gacggcgaga acatacgcgg cctttgcctg ggtcaggtcc gtatcggcgg tcagcacctc
     1321 caggtatgtc gctttgcctc gtttataaaa ttcgacaaca gccttatatg cctcgtcctg
     1381 tgcttcgacc agtcttttgg aggaccggat ggaagcgagc gccgttcgga tgtcccctaa
     1441 ggcctggctg agatcgacct gcacctggtc gtgcaccgcc ctgtcactcc actcgatctc
     1501 gcggtatcgg gcgcgcgcct tgccaacctg tgcgcgggta atgccgccct cgaacagtgg
     1561 ccactctaaa aatacgcctg ccagccattc gtcgccggca ccgcccacat cccggtcccg
     1621 atagccgtag ttcccctgga ggctgatttc cggcatatag gcccccgaag ctgagtctat
     1681 ggacaacctc gcctgctcct tgaactgcgc gctcttcttc aggtcgggat tattctcgta
     1741 catctgcgcg agcagaaact cttcatttcc aggttctgcg aagctatccg gcagtccgtc
     1801 agcaatgttg atctgcgcct caacgccgac gccgatcgtc tttttcagga tcaattcgct
     1861 gatccgcaat gcctcgtgtg cctggacaag gctcctttcc gcgtccagga gttgggcttc
     1921 tgccttgagc gattcaattc gtgttgcctt tcccgctttc tgatagctgt cggtcaattt
     1981 gaagaacgct tcccgctggg accttgaatt ctcgccgaca cgtacaagtt cgcgcgcctc
     2041 caatacccgg tagtaggcct ggctcacggc atagaccgta tcgagccggg tcgcttcggc
     2101 ggagtcccgc tgcgcgtcgg ctgcttttga tgctgctcgg ccgctatatc tggtcttccc
     2161 gccgtcatag aggagttgct tcaacttgac ctgcccaagg gattcctcgg tatactgcat
     2221 gatcggctgt atgccgacgc gatcccggtt gaccctagtg taaaaggcgc ttgcggtaat
     2281 tttgggatac aggcctgccg aagcaatgct atagtcgttc tccagttcta tgatcttctc
     2341 ctgctcgatc tgcagaccgg gattgtttgc aagggcgatc tcgatgcact ggtcaagggt
     2401 aaggagcctc ggggacgttg taccgtttcc cggcagagcg tcggctttaa ccgtctcagc
     2461 tccagccgtc tgtttggatg gcgaaccagg caaccgcctt tccgctgcgc cggtgggggc
     2521 cgaaaaaaac gctgttataa ccgccactgc aataatggct gctatcttcc ggcctcgcgc
     2581 cccgtcaaaa aaagccggga tacgttgtaa aattcgacag gtcatctttg ttctcctgtc
     2641 tgattaaacg ggcgaccttc ccttttgttc cgtaaaatat ttctgacgga tggatttgct
     2701 tctgatgaag tcgtcgacgg gaaatgcgtt cggaaggcgg gtcagctctc acagaatgat
     2761 gaagttgata tgaacacagg ggaagcgttc acgtcaaacg atgtttcttc gggcatttcg
     2821 ttgattcgct taaggacgaa tattgccttg acagagaaca gccggtttta ataaccagtg
     2881 gatccacatc gatgagcctc gagtgtgacc ggcgcagtca ggacgcggca agtccgatga
     2941 gacaacttca ccctttaaag cattcctgga tcagatcagt ggtgcaaatc ccccggcggg
     3001 catccgacct ggccggtctg cgccatggtg ttttcaatca agtcaaaaaa cttctgctgc
     3061 tggtccctgt cgagggaggc cttgacctcc agcatgtgcg ccgctattct ctcctgcatt
     3121 tcctcctgca ttctgttgat tccggcaatg gcggcattca cggctttttg atcggggttg
     3181 tcctgccgca ggagcgcgat caacgccttt cttttatctc cgatttctat gcttcgcttg
     3241 tcgatttctg cccggaaggg taacgtccgc tccctcaggg ccttgagctg ttcaggtttc
     3301 aaggacagct cttcgaacag gaacctccct ttttccatct tcgtgccgaa gggggaggtc
     3361 caagtccggg actgctgata ataggtatat cccgcagcag taaggaacga aagattcagg
     3421 accagcgagg cggcaagggc gaacttcacg aacgcgctac ttttcatggt tcgcctccgt
     3481 catggcaaga tatgcgcccc ccagagaacc cggaggcgcg gcatcgaaga ggtcaaggga
     3541 catcgaggac gccaggctgg tcgcgttctg agaaggaaga cctttcatga tgaacgaacc
     3601 agccgcgata ccgataaaaa tgacgaagag caggacagcc gcttccgcga atttggcgaa
     3661 c
//
LOCUS       DASSLD010000275         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1341657, whole genome shotgun sequence.
ACCESSION   DASSLD010000275 DASSLD010000000
VERSION     DASSLD010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341657"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..605
                     /locus_tag="VFK23_04965"
     CDS             90..605
                     /locus_tag="VFK23_04965"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7318470.1"
                     /translation="MARKYAVSAIAIFTVFTVLGSGISVAKPNAGNPITAQVPTFADR
                     SKIPASGKQGPPAKAATITGKWKPGEALFKKWCQSCHGPRGTDKVKNPGSDDGTVPPL
                     NPIDPAFASKTPSAFAANIDRIIQHGSIPDGPKPELFMPNWGDSRALSQQEIADVEAY
                     VMRLNGVRKGK"
     gene            766..1611
                     /locus_tag="VFK23_04970"
     CDS             766..1611
                     /locus_tag="VFK23_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005982928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7318471.1"
                     /translation="MLDEDKRELSKGGAGRHLGAFLTAPFAFFGRLAILWINSSGAAV
                     IFFLLAFQKIFRPKQLAKIVQQVYYIGARSSLIIMLVGLFTGMVLGLQMHSALVNVGA
                     QGYLGTIVALSLTRELGPVLTAVMITARAGSAITAEIGIQRISEQVDALSTMRIDPLR
                     YLVSPRIAAAILSFPVLTAMFDFVGIIGGYISGVMIMGANSGTYIYRVQASVEMKDII
                     HGFVKAVVFAVVVSTVCCFQGYFAHMREDSHGAKAVGLATTSAVVLSCVLILLSDYIV
                     TSLLI"
     gene            1622..>2159
                     /locus_tag="VFK23_04975"
     CDS             1622..>2159
                     /locus_tag="VFK23_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7318472.1"
                     /translation="METPLIEFKDVTKRFEGRTVLENVNLQIFEGQVTTIIGLSGSGK
                     SVLLKHIIGLMKPDDGTILFRGKPLSTVKKSETSSLRISYMFQDNALFDSMTVYENIA
                     LPLRETTNLDKAEIDRRVMSRIEQTELGEAAQRYPSELSGGMQKRAALARALVVDPQI
                     VLFDEPTSGQDPVRKNAIL"
BASE COUNT          480 a          633 c          570 g          476 t
ORIGIN      
        1 gaaggtatga ggtatcataa cttcctggca aacggtgtcg ggctctatgc tgaacgtcgc
       61 actcgattcc aacgaagggg ggatccatca tggcgaggaa atatgccgta tccgctattg
      121 cgatcttcac ggtgttcacg gtcttgggat cgggaatctc cgtggcgaaa ccgaacgccg
      181 ggaatcccat taccgctcag gtgccgacct tcgcagatcg atccaagatc cccgcgtccg
      241 gcaaacaggg acctcccgcc aaagccgcca cgattacggg aaaatggaaa ccaggggagg
      301 cccttttcaa gaaatggtgt cagtcctgcc acggcccccg gggcaccgac aaggtgaaaa
      361 atcccggctc cgacgacgga accgtgccgc cgctcaaccc catcgacccc gctttcgcga
      421 gtaaaacccc gtccgccttt gcagcgaaca tcgatcggat catccagcat ggctccattc
      481 ccgatgggcc caaaccggag ctcttcatgc cgaattgggg ggacagccgg gccctttccc
      541 aacaggagat cgcagacgtc gaagcctatg tcatgcgcct aaatggggtt cgcaagggaa
      601 aatagtgtcg aacaagtcgc cgacgtcact ttccgggacc gaagtattac gcgacaaggt
      661 ccttgtggta acatattaaa atgtatagtt ttagtgcgaa atcggggatt ctatctgcat
      721 ctcacaccgg aatgaatttc ctgcgccagt ccaggccctt tcaagatgct tgatgaagac
      781 aaacgagagc tctccaaagg tggggcgggg cggcatcttg gcgcattcct gaccgctcca
      841 tttgcctttt tcggaaggtt ggcgatcctc tggatcaaca gttcaggcgc cgcagtaatt
      901 ttcttccttc tggcatttca gaagatattc cgacccaagc agttagccaa gatagtccag
      961 caggtctatt acatcggcgc acgatccagc ctgatcatca tgctcgtcgg tctgttcacg
     1021 ggcatggtcc ttggcctcca gatgcacagt gccctggtga atgtcggcgc gcaagggtat
     1081 ctcggcacca ttgtagccct gtctctcacc agggaattgg ggcccgttct taccgccgtc
     1141 atgattacgg caagggcagg ttcggccatc accgcggaga tcggtatcca acgcatctcc
     1201 gagcaggttg acgccctctc caccatgcgc atcgacccgc ttcggtacct cgtcagcccc
     1261 agaatcgccg ccgccatctt aagcttcccc gtgctcaccg ccatgttcga cttcgtaggc
     1321 attatcggag gctacatttc cggagttatg atcatggggg caaattccgg cacgtatatt
     1381 tatcgcgttc aggccagcgt ggagatgaaa gatatcatcc acggtttcgt caaggcggtg
     1441 gtcttcgccg tcgtcgtctc caccgtctgt tgttttcagg ggtattttgc gcacatgcgt
     1501 gaggacagtc atggcgccaa ggccgtcggt ctcgcaacaa cctcggccgt cgtgctttcc
     1561 tgtgtgctca ttctgctgtc cgactatatc gtgacctcac ttctcatctg aagacaggcc
     1621 catggaaacg ccgttaatcg aattcaagga tgtgaccaag cgcttcgagg gccgaaccgt
     1681 ccttgaaaac gttaatctgc agatttttga ggggcaggtc acgacgatca tcggcctcag
     1741 cggttccggc aagagcgtgc tcctcaaaca tatcatcgga ctcatgaaac ccgatgatgg
     1801 gaccattctt tttcggggta aaccgctttc cacggttaaa aaatcggaaa catcctcgct
     1861 ccggatcagc tacatgtttc aagacaatgc gctctttgac tccatgaccg tgtatgagaa
     1921 tatcgccctc cctcttcggg aaacgacgaa cctggacaaa gcggaaatcg accgccgggt
     1981 catgtcaaga atcgaacaga cggaacttgg cgaagcggcc cagaggtacc cctcggaact
     2041 ctccgggggc atgcaaaagc gtgcggccct ggcgcgggcc ctggtcgtcg atcctcaaat
     2101 cgtcctcttt gacgaaccca cttccggaca ggaccctgtc cggaagaatg ccattctga
//
LOCUS       DASSLD010000276         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1374280, whole genome shotgun sequence.
ACCESSION   DASSLD010000276 DASSLD010000000
VERSION     DASSLD010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374280"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1558)
                     /locus_tag="VFK23_04980"
     CDS             complement(<1..1558)
                     /locus_tag="VFK23_04980"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7318473.1"
                     /translation="MAEQKTDDLTKPYRPYGADIKETRPYSPCRAACPAHTDVQAYVG
                     LIAQGRYNEAFEVITAPNPIASVCSMICHHPCEQSCRRCGVDEPLSVRHLKRFAIEKS
                     TDYRRAKRRLVAKTRNKSVGIIGSGPSGLTAANDLANLGYTVTIYERHPALGGMLVSA
                     IPPYRLPREVLKEDIDDVVSKGIEVKTNCEIGKDIKLDDLTKKHNAVLVAIGLSQSRS
                     IPIPGVDGPGVLLAIPYLTDVAFGKKPALGKKVLVIGGGNVAMDVARSARRLGVKDVS
                     MVCLESAEEMPAWKWEVDEAVEENIKINHRWGPKAVKREGEKVLGLEVKKVKSVFDAT
                     GRFNPAYDENNTTLLKADTIIITIGQMSNISFLKGTAVKVDERGRVEWDQKTQMSSAK
                     GVFLSGEVVTGPGSAIAAVANGHRAALAMHLYCQGETIEGKLPAQEQEKEKIAQMPAD
                     LVEKIAKEPRVKIKHLAPEVRCDAMVHFEIGYDEAEALKEAGRCRGCGGGAVVDKNKC
                     MACLTCMRICP"
     gene            complement(1683..2246)
                     /gene="folE"
                     /locus_tag="VFK23_04985"
     CDS             complement(1683..2246)
                     /gene="folE"
                     /locus_tag="VFK23_04985"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET7318474.1"
                     /translation="MDIKKIEKGVKLIIEGIGEDPNRPGLKSTPQRVAEMYTEIFSGI
                     SLNTAQLLKPMAGESHDEMVMIRDIPFYSVCEHHLLPFFGKAHIAYIPGAGKIVGIGS
                     LAKALEVFAKRPQVQERLTAQFIDLIVQRLRPKGAMVVIDAEHLCMSMRGVKKPGARV
                     VTSAVRGSFRSKESTRMEMLELLTKRD"
     gene            complement(2355..>2680)
                     /locus_tag="VFK23_04990"
     CDS             complement(2355..>2680)
                     /locus_tag="VFK23_04990"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET7318475.1"
                     /translation="KDFDTDVSPGTWGIQEPFESRPVPLTEIDLIITPGAAFDESGNR
                     LGYGAGYYDRMLPSFPGMTVALAFEAQIVPQVPADPHDVPVKKIVTEKRVIDPSIKQQ
                     APNLK"
BASE COUNT          506 a          808 c          751 g          615 t
ORIGIN      
        1 aggggcagat acgcatgcag gtgaggcagg ccatgcactt gttcttgtcc accaccgcgc
       61 cgcctccgca gccccggcac cggcctgctt ccttgagtgc ctcggcctcg tcgtagccga
      121 tctcgaaatg gaccatggca tcgcagcgga cctcgggggc gagatgcttg atcttgaccc
      181 gcggttcctt agcgatcttc tctaccaggt ctgcgggcat ctgtgcgatc ttttcctttt
      241 cctgttcctg tgcggggagc ttcccttcaa tcgtctctcc ctggcagtaa agatgcatcg
      301 caagcgccgc acgatggccg tttgccacgg cggcaatggc cgaacccgga ccggtcacga
      361 cctcaccgga caggaacacg ccttttgcgc tgctcatctg ggtcttctgg tcccattcca
      421 cccggcccct ctcatccacc ttgacggcag tccctttgag gaacgagatg ttcgacatct
      481 ggccgatggt tatgatgatc gtgtccgctt tgaggagcgt cgtattgttt tcgtcatagg
      541 cggggttgaa cctgcccgtg gcgtcgaaca cggatttaac cttcttcacc tcgaggccca
      601 ggactttctc gccctcgcgc tttaccgcct tggggcccca gcggtggttt attttgatgt
      661 tttcctcgac ggcctcgtca acctcccatt tccaggcggg catctcttcg gcgctctcca
      721 ggcagaccat cgaaacgtcc ttgacgccaa gcctgcgggc gctgcgggca acgtccatgg
      781 caacgtttcc gccgccgatc acgaggacct tcttcccgag tgccggcttc tttccgaacg
      841 ccacgtcggt caaatagggt atggcgagaa gcacacccgg accgtccacg ccggggatgg
      901 ggatcgatcg gctctgggaa aggccgatag ccacgagcac ggcgttgtgc ttcttcgtaa
      961 gatcgtcgag cttgatatcc ttgccgatct cgcagttcgt cttcacctcg atgcccttcg
     1021 aaaccacgtc gtcgatatct tccttcagaa cctctctggg cagccggtag ggggggatgg
     1081 ccgagaccag catgccgcca agggccggat gacgctcgta gatcgtaacg gtgtagccca
     1141 ggttagcgag gtcgttggcc gccgtgagcc cggatgggcc ggaaccgatg atgccgacgc
     1201 tcttgttcct tgttttcgcc acgagtcttc gctttgcgcg gcggtagtcc gtggacttct
     1261 cgatggcgaa gcgcttgagg tgacggaccg acagaggctc gtccacgccg cagcggcggc
     1321 acgactgctc gcaggggtga tggcagatca tcgagcagac cgacgcgatc gggttcggcg
     1381 cggtgatgac ttcgaacgcc tcgttatacc ttccctgggc gatgaggccc acgtaggcct
     1441 gcacgtcggt atgcgccggg cacgccgccc ggcaggggga gtagggtctt gtttccttta
     1501 tatcggcgcc atagggccgg tacggcttcg ttaaatcgtc tgtcttctgt tccgccataa
     1561 cactctcctg ccttggattc acattaactt tgtattatct gacattaaag tgaaaataga
     1621 aacaaaaaaa taatcgttac cgcgtctcga aactctttga tttgctactt ttggccttcc
     1681 cgtcaatccc gcttcgtcag cagttccagc atctccatgc gcgtggattc cttgctgcgg
     1741 aagctgcccc gaaccgccga agtgacgacg cgggcgcccg gtttctttac accccgcatg
     1801 ctcatgcaca ggtgttccgc gtcgatcacg accatggcgc ctttcggcct gagccgttgg
     1861 acgatcaggt cgatgaactg cgccgtcagc ctctcctgga cctgaggcct cttggcgaac
     1921 acctccaggg ccttggccag gctgccgatg cccacaatct tacccgcgcc ggggatatag
     1981 gcaatgtgcg ctttgccgaa gaacggaagc agatggtgct cgcagaccga ataaaacggg
     2041 atatcgcgga tcatcaccat ctcgtcgtgg ctctctccgg ccatgggctt gaggagctgc
     2101 gccgtattca gggaaatacc ggagaagatc tccgtataca tctccgccac gcgctgcggg
     2161 gtactcttga gccccggccg gttcggatcc tcgccgatgc cttcgatgat gagcttaacg
     2221 cccttttcaa tcttcttgat atccatgcga gaaaaaagaa aaaacaaatt tcaaatccca
     2281 gatatcaaac aattccggta ttcaggggat caatgaccga aacgaatgaa gtttcgaatc
     2341 gtggagcttg aattttattt gagatttgga gcttgttgtt tgatgcttgg atcgatgacc
     2401 ctcttctccg tcacgatctt cttgaccgga acatcatgcg gatcagcggg gacctgcggt
     2461 acgatctgcg cctcaaaggc aagcgctacg gtcatccctg gaaaagaagg gagcatcctg
     2521 tcgtaatacc cggcgccgta accgagccgg ttgccgctct catcgaacgc cgcgcccggt
     2581 gttatgatga ggtcgatctc cgtaaggggc acaggacgcg attcaaaggg ctcctgaata
     2641 ccccaggtgc cgggagacac atccgtgtca aagtccttga
//
LOCUS       DASSLD010000277         2816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_90609,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000277 DASSLD010000000
VERSION     DASSLD010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90609"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..477
                     /locus_tag="VFK23_04995"
     CDS             184..477
                     /locus_tag="VFK23_04995"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HET7318476.1"
                     /translation="MKKKRIILIVPILTSVLVTLATAKMYQYTDKNGVIVITDNPAAG
                     SNAGEVKMKNDRVFRSAPRRSEPAPPADSAPAEEQRRKRGYSDVNVVMYMTSW"
     gene            490..693
                     /locus_tag="VFK23_05000"
                     /pseudo
     CDS             490..693
                     /locus_tag="VFK23_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
     gene            713..2107
                     /locus_tag="VFK23_05005"
     CDS             713..2107
                     /locus_tag="VFK23_05005"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7318477.1"
                     /translation="MSIRTIRFLGAAREVTGSCYLLTLPKRSILVDCGIVQGASDSRD
                     RNREPFAFDPAGIDRLFLTHAHLDHSGLIPRLVKEGFRGRIVTTTATADLIGPMLLDS
                     AKIQENDAERMSMRSLRKGGETVEPLYTTEDVEKVLPLIDRAKYGQVTDTGMGVRYCF
                     LDAGHILGSGSLALWLDTTAGAKKIIFSGDIGRKGAPIINDPVLATEADYVVMESTYG
                     NRLHKNSADTTRELAEAITTTFTKSGNVLIPAFAIGRTQDVLYLLNKLVREGTLPKLT
                     VTIDSPLAEKATQIYLRHPELYDEEAKRLIAGNRIGDAIDIRFTHSAEESMALNKIKS
                     GSIIIAGSGMCEGGRIRHHLKHNLWRPECSIIFVGFQAQGTLGRRIVDGAQEVTIWGE
                     EIAVRARVWTIGGFSAHGDQKELLDWLSAFRSRPTVFVTHGEESAALTFADLVRKRYG
                     FTTHVPEPGSQFAL"
     gene            2154..>2816
                     /locus_tag="VFK23_05010"
     CDS             2154..>2816
                     /locus_tag="VFK23_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HET7318478.1"
                     /translation="MKKIAVNGLGRIGRLVLRQYLSNKPEHVTLVAVNSPAPVEELAY
                     LIKYDSVHGRARFDIKTGPDYLQLGSTRVAVSHERDPEILPWRALGVDIVLECTGLFL
                     KREDATKHLTAGASKVIISAEAKNEDLTVVLGVNEKAYNPQKHHILSNASCTTNCLAT
                     AVKVLNDAFGIDYLMATTIHAYTASQALIDSSSPKHRRRGRAAAVSLIPSSTGAATAT
                     EKV"
BASE COUNT          656 a          819 c          849 g          492 t
ORIGIN      
        1 agggaaggca cgctgaagta ctttgactgg tatgacaact ccgatcccaa acccaacaag
       61 tagtaaatgc ggaattcgga ctgccaaatg cggagataaa tcataaaaat aggcgactgg
      121 aagcggcgac ggcagttcag gcggatttcc gctgtaagga atcatggcga aggggagagg
      181 acgttgaaga aaaaacggat cattctgata gtcccgattt taacgtctgt gctcgtcacg
      241 cttgcgacgg cgaagatgta ccagtatacg gacaagaacg gcgtcattgt gattaccgac
      301 aatcccgctg cagggtcgaa cgcgggagag gtcaaaatga aaaatgaccg ggttttcagg
      361 tccgcgccga ggcgctccga accggccccc ccggcagact ctgcgcccgc ggaggagcag
      421 aggcgcaagc gcggctacag cgatgtgaac gtagtcatgt acatgacaag ctggtgaggg
      481 tattgcaaaa aggcgggcgt gtacgtccgc tcactcggcg caaaccttgt ggaacataat
      541 atcgagaccg atcaggagaa gaaagaagaa atgaaaagaa aaagcggggg ttccacgtct
      601 gtcccgcttc tggacattga gggccagatc atccgcggtt tcagccagga tgcgataaag
      661 gccgccatag acaggagtct ggtgcggcga tgacctgggc aaggaggcca tcatgagcat
      721 ccgtacgatc cgttttctcg gtgcggcacg agaggtgacc ggctcgtgct acctgctgac
      781 tttgccgaag agaagcatcc tcgttgactg cgggatcgtg cagggcgcga gcgactcccg
      841 cgaccggaac cgcgagcctt ttgcctttga ccctgcgggg atcgaccgcc tctttctgac
      901 ccacgcccac ctcgaccact ccggcctgat tccccgcctg gtgaaggagg gtttccgggg
      961 caggatcgtc acgaccacgg ccacggcgga cctgattggc cccatgctgc tcgactcggc
     1021 aaagatccag gagaacgacg ccgagcggat gtccatgcgg tcgctgcgga agggcggcga
     1081 aacggtggag ccgctttaca cgaccgagga cgtggagaag gtgttgccgc tcatcgatcg
     1141 tgccaagtac ggccaggtca ctgacaccgg catgggcgtc cggtattgct ttctcgacgc
     1201 aggtcacatt ctaggctcag gcagcctggc cctgtggctc gatacgaccg ccggcgcgaa
     1261 gaaaatcatc ttctcgggcg acatcggccg gaagggggcc cccatcatca acgaccccgt
     1321 gctggcaacg gaggccgact acgtggtcat ggaatccacc tacggcaacc ggctgcacaa
     1381 gaacagcgcg gataccaccc gggaactcgc cgaggcgatc accactacgt tcacaaagag
     1441 cggaaacgtg ctcatccccg cctttgccat cggccgcacg caggacgtcc tatacctgct
     1501 caacaaacta gtgcgcgagg ggacgctgcc gaaacttacc gtcacgatcg acagcccgct
     1561 cgcggaaaag gcaacgcaaa tctacctcag acacccagaa ctctatgacg aggaggcgaa
     1621 gcggctgatc gccggcaaca ggatcggcga tgccatcgac atccggttca ctcactccgc
     1681 cgaggaatcc atggccctca ataagatcaa gtccgggtcc ataatcatcg ccggcagcgg
     1741 catgtgcgag gggggacgca tcaggcatca cctgaagcac aacctgtggc ggccggagtg
     1801 cagcatcatc ttcgtcgggt tccaggcgca gggcactctc ggccggcgga tcgtggacgg
     1861 tgcgcaggag gtcacgatct ggggcgagga gatagcggtc cgtgcacggg tctggaccat
     1921 cggcgggttc tcggcccacg gcgatcagaa ggagctgctg gactggcttt cggcgttcag
     1981 gagcaggccg acggtgttcg tgacccatgg tgaagaatcg gcagctctca cctttgcgga
     2041 cctggtgcgg aagcggtacg gattcacgac ccacgtgccc gagcctggat cgcaattcgc
     2101 tctgtaaaga cacgtatggc cgcatcgaaa catacgcatt aaggaggcct ttcatgaaaa
     2161 aaatcgccgt taacggtctc gggcgcatcg gacgcctggt cttgcgtcag tatctttcga
     2221 acaagccgga gcacgtgacc ctcgttgcgg tcaacagccc ggccccggtc gaagagcttg
     2281 cctaccttat caaatacgac tctgtccacg gacgggcccg cttcgatata aaaacaggcc
     2341 cggattatct ccaactcgga tcgacgagag tggcggtttc ccatgaaagg gaccctgaaa
     2401 tattgccttg gcgggccttg ggtgttgaca tcgttttgga atgcaccggc cttttcctca
     2461 aacgggaaga cgcaaccaaa cacctgacgg ccggcgcatc gaaggtcatt atcagcgctg
     2521 aagcgaaaaa tgaagacctg acggtggtcc tgggagtgaa cgaaaaagcc tacaatcccc
     2581 aaaaacacca tattctctcc aacgcgtcct gtacgacgaa ctgtctggca acggccgtca
     2641 aggtgctgaa tgacgccttc gggatcgatt acctgatggc cacgacgatc catgcctata
     2701 ccgccagcca ggccctcata gacagctctt ccccgaagca ccggaggcgg ggacgggccg
     2761 ccgctgtttc gctgataccg tccagcaccg gggccgccac ggccacggag aaggtg
//
LOCUS       DASSLD010000278         3611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1408387, whole genome shotgun sequence.
ACCESSION   DASSLD010000278 DASSLD010000000
VERSION     DASSLD010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408387"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VFK23_05015"
     CDS             complement(<1..917)
                     /locus_tag="VFK23_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7318479.1"
                     /translation="MNIVVIRPTYNEKDNIGLMIDALREQFRRIPHDMSILVVDDNSP
                     DGTADVVRAHSGTARDLFLITGKKEGLGAAYIRGMKHAIQELHADAVMEMDADFSHKP
                     EDVPRLIEELDRGADFVIGSRYVPGGKIPEDWGFLRRMISRWGNVFARYVAGMYGVRD
                     CTAGFRAIRSSLIDKIDPDTLRVKGYAFQISLLHEAILNRAVVREVPVEFVDRERGQT
                     KLGLSDIVEFMLNAWWIRLERSKTFLKFSVVGAIGVVVNLGSFTVLMNLGLNKYLASP
                     VAIEISIIANFLMNNYWTFAKRDMNSRVH"
     gene            complement(914..2092)
                     /locus_tag="VFK23_05020"
     CDS             complement(914..2092)
                     /locus_tag="VFK23_05020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HET7318480.1"
                     /translation="MPVEGKKAVRTAMAGWIVLFLAVSAIVVLDGKRSVVPSYRAGAL
                     SWIGGQPLYDGTGVGDFVYLPQAAVLYVPFALFPPVAGEVLWRLGMIAVFAAGLYRFG
                     RLAGERSEGEFFPLLTVVAIPLVWDCARNGQATLAITGLMLLAVVDREQGRLWRATAW
                     LSLGIAIKPLVLVLALLVMATDRRMSWRLLAGMGVTALVPFLTQSPGYVVQQYAACVE
                     NMTTAAHVGVSVRGWTSPFTALRALGIADVPENIQTTIRVGGAAATLLLCFSLKRRLE
                     PSRAALYLFTLSVLYLMLFSPRTENNTYSMLSPALAAFLASAYFDENRRGEALLIASL
                     SLLLAGSRIVERLVAPHAEQVWLSPAIAVAFAAYTLVRLFLDAAHPLTGEKVPHTGPV
                     "
     gene            complement(2117..2650)
                     /locus_tag="VFK23_05025"
     CDS             complement(2117..2650)
                     /locus_tag="VFK23_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7318481.1"
                     /translation="MKKTAGVLTVVFVVMMSFSCQQRQEQKQQPVVYTPAAPPAQMQI
                     DQYQAAVKQSPKSAPAWINLGDVLMDAQRFAEAIEAYDKALALDPKNVNVLVDQGTCY
                     RGIGKFDKAVEMYQKAIKIDPKFPNAHRNLGVVLSIDLHKNAEGAKEFQKYLELKPDA
                     HDAESIRQTIRQLEARK"
     gene            complement(2788..3105)
                     /locus_tag="VFK23_05030"
     CDS             complement(2788..3105)
                     /locus_tag="VFK23_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HET7318482.1"
                     /translation="MSEFKNVSVVREANIYFDGKVTSRTVLFPDGSKKTLGVMLPGDF
                     EFGTADKELMEILSGELDVLLPNETAWKPVKGGQSFEVAANSKFKLKVKKLTDYCCSF
                     IKG"
     gene            3332..>3611
                     /locus_tag="VFK23_05035"
     CDS             3332..>3611
                     /locus_tag="VFK23_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET7318483.1"
                     /translation="MDKIAKRQVIVIGKYSAWIVLPLLAFLALPAIMAPAEEGPWEVK
                     KPAPVERSEVAAAALDNRVYVIGGFLREGIGDLVEEYDPAEDRWTERAP"
BASE COUNT          740 a         1105 c         1020 g          746 t
ORIGIN      
        1 atgtggaccc tgctgttcat gtcccgcttc gcaaaggtcc agtaattgtt catcagaaaa
       61 ttcgcgatga tcgatatttc gatcgcgacg ggcgaggcga ggtacttgtt cagtcccaga
      121 ttcatgagca ccgtgaacga gccgaggttc acgacgacgc cgattgcgcc gactacggag
      181 aatttcagga aggtcttgct ccgctcgagc cggatccacc acgcattcag catgaattca
      241 acgatgtccg agaggcccag tttcgtctga ccccgctccc ggtccacgaa ttcaaccggg
      301 acttcccgca ccactgcccg gttcaggatc gcctcgtgca gaagcgatat ctggaacgcg
      361 tagcccttga cccggagcgt atccgggtcg atcttgtcga taagggaaga ccgtatcgcc
      421 cggaaccccg ccgtgcagtc cctgaccccg tacatccccg ccacatagcg ggcgaacacg
      481 ttcccccacc gggagatcat ccttcgcagg aaaccccagt cctccggtat cttcccgcca
      541 gggacgtacc tgctgccgat cacaaagtcc gctccgcggt ccagttcctc gatgaggcgg
      601 gggacgtctt ccggcttgtg ggagaagtcc gcgtccatct ccatcactgc gtcggcatgg
      661 agctcctgga tggcgtgctt catgcccctg atataagccg cccccaggcc ttcttttttc
      721 ccggtgatga gaaaaagatc ccgcgccgtt cctgagtgcg ccctgacgac atccgctgtg
      781 ccgtcgggcg agttgtcgtc gacgaccagg atgctcatgt cgtgcgggat tctccgaaac
      841 tgctcccgga gcgcgtctat catgagcccg atattgtctt tttcgttata ggtcggacgg
      901 atgactacta tgttcataca ggacccgtat gaggaacctt ttccccggtg agaggatgtg
      961 ccgcgtccag aaacagccgg accagcgtgt aagcggcaaa cgccacggct atggccggtg
     1021 acagccatac ctgctccgcg tggggcgcga ccagccgctc gacgatcctg ctgcctgcca
     1081 acagaagcga aagcgaggcg atcagcaggg cctcccctct ccggttttcg tcaaagtacg
     1141 ccgaggcaag aaaggcggcg agcgcgggac tgagcatgga gtaggtgttg ttctcggtcc
     1201 gcgggctgaa cagcatgaga tagaggaccg aaagcgtaaa gaggtagagc gctgcgcgtg
     1261 acggctccag gcgccgcttc agggagaaac acagaagcag cgtagccgcg gccccgccga
     1321 cccggatcgt ggtttggatg ttttccggca cgtcggcgat gccgagagcc cggagcgccg
     1381 tgaacggaga cgtccacccc ctgaccgaga cgcccacatg cgccgcggtc gtcatgttct
     1441 ctacgcaggc tgcatactgc tgcaccacat agcccggcga ctgcgtgaga aacggcacga
     1501 gggcggtcac tcccatgccc gcaagcagcc gccagctcat cctgcggtcg gtcgccatga
     1561 ccaggagcgc gagcaccagc accagaggct tgattgcaat gccgagcgac agccaggccg
     1621 tcgcccgcca aagacggccc tgctccctgt ccaccaccgc aagcagcatc aggccggtga
     1681 tcgcgagcgt tgcctggccg tttcgggcgc aatcccatac cagcggaatg gctactactg
     1741 tcaggagagg gaaaaactcc ccctccgagc gttctcccgc gagcctgccg aaccggtaga
     1801 gtcccgccgc aaacacggcg atcatgccca gccgccacag cacctcgccg gcaaccgggg
     1861 ggaaaagcgc aaagggcacg tacagaacgg cggcctgcgg aagatagacg aagtcgccta
     1921 cgccggtccc gtcatacagg ggctgtccgc cgatccagct cagcgcgccg gcccggtaag
     1981 agggaacaac ggaccgtttg ccgtcgagga caacgatagc ggagaccgcg agaaacagca
     2041 cgatccatcc ggccatcgcg gtccgcaccg cctttttccc ctccaccggc atcttatgtt
     2101 ccttccgggc gcatttttat ttgcgggctt ccaattgacg aatcgtctgc ctgatcgact
     2161 ccgcgtcgtg tgcatcaggc ttcagttcga gatatttttg gaactctttg gccccttccg
     2221 cgttcttatg caggtctata ctcaggacaa cgccgaggtt ccggtgggca ttggggaatt
     2281 tgggatcgat ctttatcgcc ttctggtaca tttcaacggc cttgtcgaat ttgccgatgc
     2341 cgcggtagca ggtcccctgg tccaccagca cgtttacgtt tttcggatcg agggcaagcg
     2401 ctttgtcgta ggcttcgata gcctcggcga agcgctgcgc atccatgagt acgtcgccga
     2461 gattgatcca agccggcgcg ctcttgggcg actgcttcac tgcagcctgg tactggtcga
     2521 tctgcatctg cgcgggcggg gctgcgggcg tgtacacgac cggctgctgc ttctgttcct
     2581 gccgctgctg gcatgagaag gacatcatta cgacgaacac gacggttaaa acacctgcag
     2641 tctttttcat acatcctccc aaatccgaca aaatgataaa agacgaaaaa gcgatttttt
     2701 accgcagagg cacagaggaa accacttcaa attttcaagt tttttctccg tgtctccgcg
     2761 tctccgcggt agatcttgac tcttgtgcta tcccttgatg aacgaacagc agtagtccgt
     2821 cagctttttt accttcaatt tgaattttga gtttgccgcc acctcgaagg actggcctcc
     2881 cttgacgggt ttccatgccg tctcgttcgg caggagcacg tcgagttcgc ccgacaggat
     2941 ctccatgagc tccttgtccg cggtgccgaa ctcgaaatcg cccggcagca tcacgccgag
     3001 agtctttttg gacccgtcgg gaaaaagcac ggtcctgctc gtcacctttc cgtcgaaata
     3061 gatgttcgct tccctcacta cgctcacgtt tttgaactcg gacatgcatg gcccctttct
     3121 tacggtatcc cgcactgtct tttgtacact gtctcgccgt gccctgtcaa ccataaaaaa
     3181 agtttcccgc tacgagtttc gcgtcaacgg cataattatg ccgttgacgg aagacgggaa
     3241 acttaagcac cggggttgtt taagcaactt cttgacaacc tgcaacaccg gaactagatt
     3301 ttaattaaga tcagaaaggc gttccgaggc gatggacaag atagcgaaga ggcaggttat
     3361 tgtcatagga aagtattccg catggattgt tctgcccctg cttgcgttcc tggcccttcc
     3421 ggcgattatg gccccggccg aggaagggcc gtgggaagtg aaaaagcccg cgcctgtcga
     3481 gcgctcggaa gtggccgctg cggccctcga caacagggtc tacgtcatcg gaggtttcct
     3541 cagggagggg ataggagacc ttgtcgaaga atacgacccg gctgaagacc ggtggacgga
     3601 aagagcccct c
//
LOCUS       DASSLD010000279         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1113124, whole genome shotgun sequence.
ACCESSION   DASSLD010000279 DASSLD010000000
VERSION     DASSLD010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113124"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2109)
                     /locus_tag="VFK23_05040"
     CDS             complement(<1..>2109)
                     /locus_tag="VFK23_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET7318484.1"
                     /translation="LYISPLKALNNDVQRNLLKPLRDLREYFSRAGVSFPEIRVLTRS
                     GDTPGEERRKMIKRPPEILITTPESLNLLLSSRSGRSLLGGIATVILDEIHEVAGTKR
                     GTHLITAVDRLVPLTGEFQRIALSATVNPPGRVADFIGGSRKIGKGKDSHYEKRPVSI
                     IRSSTAKDYELRVSFPPDAREKTADGSWWPALIDAFRENIFRHRSTLLFANSRRTTEK
                     VTRLINERSPSELAYAHHGSLAREIRLAVEQKLKNGELRAIVATNSLELGIDIGDLDQ
                     VVLIQTPRSVSSALQRIGRSGHSVGEVSRGLLFPTHGRDFIDAAVMARAILDQDIEEV
                     RPIEAPLDILAQVILSMTAMERWDIDDLYAFLKTSYPYRNLPRRHFDLVLEMLAGRYA
                     DSRLRELKARVSLDRVDNTVQAHSEAPYLIYTSGGTIPERGYFDLRLKDTRAKIGELD
                     EGFVWERSLGETFTLGTQLWRIEKITHNDVEVVPVRSTAGIFPFWKAEEQDRDFHFAE
                     RVGLFLERADARLDSPGFKQELLERHAMEEAAAAELIGFLKLQKEASGTELPHRHHLL
                     IEQVEDPASRGDLKQVILHTFWGGRVNRPFALALSQAWEEKERKPLQFILNDDAVLLM
                     IPQEFRTEDLFSLVTPENIEALLRRKLEQTGFFGARFRENAERALLLPKSGFKRRMPL
                     WLIRLRSKELLAAVKAYGDF"
BASE COUNT          369 a          646 c          688 g          406 t
ORIGIN      
        1 ggaagtcccc ataggctttc acggctgcga gcagttcttt cgacctgagc cggatgagcc
       61 agagcggcat cctccgcttg aagccggatt tggggagcaa gagggcccgc tccgcgttct
      121 cccggaaccg ggcgccgaaa aatccggtct gctcgagctt cctgcgcagc agggcctcga
      181 tattctccgg cgtgacgagg gaaaagagat cctcggtcct gaactcctgc ggtatcatga
      241 ggaggactgc gtcgtcgttc aggatgaatt gaagaggctt tctctccttc tcctcccacg
      301 cctgggagag cgccagggca aagggccggt tcaccctgcc gccccagaag gtatgcagga
      361 tcacctgctt caaatcgccc cggctagcgg ggtcttcgac ctgttcgatg aggaggtgat
      421 ggcggtgcgg gagctccgtg ccggacgcct ccttctgcag tttcagaaac ccgatgagct
      481 ccgctgcggc cgcctcttcc atggcgtgcc gctccaggag ttcctgcttg aatccgggcg
      541 aatcgagccg cgcatccgcg cgttcaagaa agaggccgac ccgctcggca aagtggaaat
      601 cgcggtcctg ctcctcggcc ttccagaacg ggaagatacc ggccgtcgaa cgaacgggga
      661 ccacctccac gtcgttgtgc gtgatctttt cgatgcgcca gagctgggtg ccgagggtga
      721 aggtctcgcc cagggaacgc tcccatacga acccctcgtc cagttcgccg atcttggcgc
      781 gggtgtcttt gagccgcagg tcgaagtagc cgcgctcggg aatggtgccg cccgacgtat
      841 agatgaggta aggcgcttcg ctgtgcgcct ggaccgtgtt gtccacgcgg tccagcgaga
      901 cccgtgcctt gagttccctg aggcggctgt ccgcatagcg gccggcgagc atttcgagaa
      961 cgaggtcgaa gtgccttcgc ggaaggttgc ggtagggata agaagttttc aggaacgcgt
     1021 acaggtcgtc gatgtcccac cgctccatcg cggtcatcga gaggatgacc tgcgcaagga
     1081 tgtcgagcgg cgcttcgatg ggccggacct cttcgatgtc ctggtccagg atcgcccgcg
     1141 ccatgaccgc cgcgtcgatg aagtcccggc cgtgggtggg aaagagcagg ccgcggctta
     1201 cctcgcccac ggaatgaccg gagcggccga ttcgctggag cgcagaggag accgatcgcg
     1261 gcgtctggat caggaccacc tgatcgaggt ccccgatgtc gatgccgagc tccagcgagt
     1321 tcgtcgccac gatggcccga agctccccgt tcttgagctt ctgctcgact gcaagacgga
     1381 tctcccgcgc aagcgatccg tggtgggcat aggcgagttc ggagggcgac ctttcattga
     1441 tcagccgcgt caccttctcc gtcgtcctcc tgctgttcgc gaagaggagc gtcgaccggt
     1501 gccggaagat gttctcgcga aaggcgtcga tcagggccgg ccaccaggaa ccgtcggccg
     1561 tcttctcgcg cgcgtcgggc gggaaggaaa ctctgagctc gtagtccttt gccgtggagg
     1621 accggatgat ggagacaggc cttttctcgt aatgactgtc tttccccttc ccgattttgc
     1681 gggagcctcc gataaagtcc gccacccgcc cggggggatt gaccgtcgcg gaaagcgcga
     1741 ttcgctgaaa ttctccggtg agcggaacga gcctgtccac cgcggtgatg aggtgcgtcc
     1801 cccgcttggt cccggcaact tcgtggatct catcaaggat caccgtggcg atcccgccca
     1861 gcagggagcg gccgctccgg gacgagagga gaaggttgag cgactcgggc gtcgtgatga
     1921 gaatctccgg cggccttttg atcatcttcc tccgttcctc gccgggcgtg tcgccgctcc
     1981 gggtgagcac cctgatctcc gggaaagaca cccccgcccg ggagaaatat tcgcgcaggt
     2041 cccggagcgg cttcagcagg tttcgctgga catcgttgtt caaggccttc agaggagaga
     2101 tgtagagga
//
LOCUS       DASSLD010000280         4315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1371772, whole genome shotgun sequence.
ACCESSION   DASSLD010000280 DASSLD010000000
VERSION     DASSLD010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371772"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VFK23_05045"
     CDS             complement(<1..751)
                     /locus_tag="VFK23_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318485.1"
                     /translation="MNEHTLRVLEFDKLIAIVAGYAASAAGAAAVRRAHPANRPDAVA
                     ALLAETSEFTRILESGETPPLDGIPEVGQAVEKLGIAGVVLTPAELLAVAAILAAGRR
                     IRKFFQRIEGKGTVSHPFAPLLCSQAAFIRPLKDIEDAVFAAIDERAEVRDSASPDLR
                     KLRRQIVRTRDDILDRMSGMLQDSGFQKVIQEPVITIRDDRYVLPLKPNFRQNLKGVV
                     HGQSGSRATLFVEPLEVLEQNNRLAELRMEER"
     gene            complement(845..1288)
                     /locus_tag="VFK23_05050"
     CDS             complement(845..1288)
                     /locus_tag="VFK23_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET7318486.1"
                     /translation="MGLREKIDADVKEALKSGAKDRLSTLRMLNAALKNKQIDKRRPL
                     TEDEVVETVRSLIKQRRDSVEQFAKGGRQDLVDKETAEIKVLESYLPQQLSREEIEAM
                     VRDVITSTAAQGAKDMGKVMKALIPMIGGRADGKLVSELVKNALG"
     gene            complement(1361..1561)
                     /gene="rpsU"
                     /locus_tag="VFK23_05055"
     CDS             complement(1361..1561)
                     /gene="rpsU"
                     /locus_tag="VFK23_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET7318487.1"
                     /translation="MPSIRLRENEPFEAALRRFKKSIEKEGVLSEVKKREHYEKPSVK
                     RKKKALAARKKAAKTARMYRQG"
     gene            complement(1795..3000)
                     /gene="argJ"
                     /locus_tag="VFK23_05060"
     CDS             complement(1795..3000)
                     /gene="argJ"
                     /locus_tag="VFK23_05060"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HET7318488.1"
                     /translation="MRILDTKNFFVPGYKAWGITCGIKKNEKKDLAIIFSDREASVAG
                     VFTKNRVKAAPVLLDMVKVRSGKGQAIVVNSGCANACTGKRGLADARETAEVAARELG
                     VKPDAVLVASTGVIGEFLPMAKVSTGIATAAGLLVGNGWEQAAEAIMTTDIYPKLSIV
                     QEEIGGKTATVAGIAKGSGMIHPNMATMLCFIVTDAAIAAPLLRRALISSTERSFNSI
                     TVDGDTSTNDTILCMANGAAGNKRIAAGSKDFRKFQSCLDAVMRALARQVVRDGEGAT
                     KFVEIEVRNARNPREAKQAAMTVAKSSLVKTALFGEDANWGRIMAAVGYSGVDIDEAR
                     TDIHIGKAKIVEKGLSAGKAAERDAALALKQREVQIVIDLHKGKGSATVWTCDLSYEY
                     VKINAAYRS"
     gene            complement(3075..4127)
                     /gene="argC"
                     /locus_tag="VFK23_05065"
     CDS             complement(3075..4127)
                     /gene="argC"
                     /locus_tag="VFK23_05065"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET7318489.1"
                     /translation="MTSSNVKIAIVGSSGYTGGELYRLLIHHRFATITAVTSEKSAGK
                     PLTTIFPHLAGLTDLLCEPLDPDVIAGKADFIFLALPHVTAQEAAFRFRRLGKKVVDL
                     SADYRLSDPALYEKWYEHCHQYPDLLKQAVYGLPELHREEIKAASLIANPGCYPTSAI
                     LGLAPLVKAAAIDLTTIIIDSKSGVSGAGRSPSLAHHYPEVNEGLMAYKIGTHRHTPE
                     IEQEVSTLAGKPVTLSFTPHLVPMNRGILTTIYASLQERADAGRLHALYREFYEREPF
                     VRVLAPGQFPNVRNVRGSNFCDIGVYADVRTNRAVVIAAIDNLVKGASGQAIQNMNLM
                     MGYDEREGLAGAGLFP"
BASE COUNT          830 a         1316 c         1187 g          982 t
ORIGIN      
        1 cgcgctcttc catgcgcagt tcggcaagcc tgttgttctg ctccagcacc tcaaggggtt
       61 ccacgaagag cgtggcccgg gaacctgact gcccgtgcac cacacccttc aggttttgcc
      121 tgaagttggg tttgagcggc aacacgtacc ggtcgtcgcg gatcgtaatg accggttcct
      181 ggatgacctt ttgaaaaccg ctgtcctgca gcatgccgga catgcggtcg agaatgtcgt
      241 ccctcgtccg cacgatttgc ctgcggagct tccggaggtc cggactggcc gagtctctga
      301 cctcggcacg ctcgtcgatg gcggcgaata ccgcgtcctc gatatccttg agcggccgta
      361 tgaaggcggc ctgcgagcac aaaagggggg caaagggatg ggacaccgtg cccttgcctt
      421 cgatccgctg gaaaaatttt ctgatccgtc tgcccgcggc gaggatcgcg gcaacggcga
      481 gcagttcagc cggcgtcagg acgacgcctg caatgccgag cttctcgacc gcctgcccga
      541 cttcgggaat gccgtcgagt ggcggcgttt cgccgctttc caagatccgc gtaaattcac
      601 tcgtctccgc gagaagggcg gcaactgcat ccggcctgtt tgcgggatgc gccctcctga
      661 cggcagctgc gcccgctgcc gacgcggcgt aaccggcgac gatggcaatg agcttgtcaa
      721 actcgaggac ccggagggta tgttcgttca taaaaaaaat cagggggccc cggtttccgt
      781 ctccatggcc ccctgttccg ctccgctgtg agtaaatggc acgccgtctg cgcgaaccag
      841 gtcgttatcc cagtgcattt tttacgagtt cgctcaccag tttcccgtct gcgcggcctc
      901 cgatcatggg gatgagcgcc ttcatgactt tgcccatgtc cttggcgccc tgtgccgcag
      961 tgcttgtaat aacgtcccgg accatcgcct cgatctcctc gcgcgataac tgctggggca
     1021 gataggattc aagcaccttg atttcggcgg tttccttgtc tacgaggtcc tgccgtccgc
     1081 ctttcgcaaa ctgctcgacc gaatccctcc gctgcttgat aagagaacga acggtctcga
     1141 ccacctcgtc ttcggtgagc ggccgacgtt tgtcgatctg cttgttcttg agggcggcgt
     1201 tcagcatccg cagtgtcgag agtctgtcct tcgcgccgga cttaagcgcc tctttcacgt
     1261 cagcgtcgat tttctcacgc agccccatta cgccgttccc ctcactgtgt ctcgccggac
     1321 cccgcgtcga gcaatacgac ctgcccgctc ccggcgacga ttagccctgc ctgtacatgc
     1381 gcgcagtctt agcggctttt ttgcgtgcgg ccaatgcctt tttctttcgc tttacactcg
     1441 gcttctcgta atgctctctc ttcttgacct cggaaagcac gccttctttc tcaatacttt
     1501 ttttaaatct acggagagcg gcctcgaatg gctcgttctc ccgcaatctg atgctcggca
     1561 ctatcgctac acccccctcc taatgcaaat cattgtaata ccagcatttt tccgcttcaa
     1621 tcgcaaagaa tgcggccgtg gaaataagtg cataattgcg acggtcagtg aatcggcata
     1681 atcatcaaaa ttaacaagtc caccttggaa tgtcaagtga aattttgtca gattttcagc
     1741 gctttttgtc tcagaataag ggcccccttc acggcccgaa acgacagaaa agcatcagct
     1801 ccggtaggcg gcattaatct tcacgtattc gtaggacaag tcgcaggtcc agaccgttgc
     1861 gcttcctttc cccttgtgca ggtcgatgac aatctggacc tcgcgctgct tgagcgcgag
     1921 cgccgcatcg cgttcagccg ccttgcccgc gctcaggcct ttctcgacga ttttggcctt
     1981 gccgatatgg atgtccgttc tcgcctcatc tatatccacg ccggaatatc ccacggctgc
     2041 catgattcgc ccccagttcg cgtcttctcc aaaaagcgcc gtcttgacaa ggctggattt
     2101 cgcgaccgtc atagccgctt gcttggcctc gcggggattc ctggcatttc tcacttcgat
     2161 ctccacgaac tttgtagcgc cctcgccgtc acggaccacc tgccgggcaa gcgcgcgcat
     2221 cacggcgtcc aggcaagatt gaaacttcct gaaatccttg ctgccggcgg caattctctt
     2281 gttccccgct gccccgttcg ccatgcagag tatcgtgtcg ttcgtgctcg tatcgccgtc
     2341 caccgtgatc gagttgaaag acctttccgt ggaagagatg agcgctctcc tgagcagggg
     2401 agccgcgatc gcggcatcgg tcacgataaa acagagcatt gtagccatgt tcgggtggat
     2461 cattccggaa cccttggcga tacctgcaac ggtggccgtc tttccgccga tttcctcctg
     2521 cacgatggac agcttcggat agatatcggt ggtcatgatc gcctccgcgg cctgctccca
     2581 gccgttgccc accagcaggc cggccgcggt tgcgatgccc gtgctgactt tcgccatggg
     2641 cagaaactcc ccgatgacgc cggtcgatgc aaccagcacg gcatcgggtt tcacgccaag
     2701 ttcgcgcgcc gcgacttcag ccgtctcgcg agcgtccgca aggccgcgct tccccgtgca
     2761 ggcgttggcg catccgctgt tgacgacgat ggcctgtccc ttgccggatc tgactttcac
     2821 catgtccaga aggacggggg cggccttcac gcggttcttg gtaaagacgc cggcgacgga
     2881 ggcctcgcgg tcagagaaga tgatcgcaag gtctttcttt tcgttcttct tgatcccgca
     2941 ggtgatgccc catgccttgt aaccgggcac gaaaaaattc ttggtatcca gaatcctcat
     3001 gattttaccc ctgttccttg cgaagattat gatgaatcgg ggcaacggtg atatcctccg
     3061 gtccccgttt tgcctcaggg gaacaatccc gcgccggcga ggccttcccg ttcatcgtag
     3121 cccatcataa ggttcatatt ctgaatggcc tgtcccgatg cgcccttgac gaggttgtcg
     3181 atggccgcga tcacaacggc cctgttcgtc ctgacgtcgg catacacgcc gatgtcgcag
     3241 aaattggacc ctcgaacgtt ccgcacgttt ggaaactgac cgggggcaag gacccgtacg
     3301 aagggctctc gttcgtagaa ctcacggtac agggcatgca acctgccagc atcggctcgt
     3361 tcctgcagtg aggcgtagat agtggtcaga ataccgcggt tcatcggaac aagatggggg
     3421 gtgaacgaca gcgtcacggg ctttcctgcc agcgtcgaga cctcttgttc gatctccggc
     3481 gtatgacgat gggtcccgat cttatacgcc atcaggcctt cgttcacctc gggataatgg
     3541 tgtgccagcg acggactgcg gccggcgccg gagacccccg attttgaatc gatgatgatc
     3601 gtcgtgaggt cgatggcggc cgctttcacg agcggggcaa gccccagaat agcgctcgtg
     3661 ggatagcacc ccgggttcgc aatgagcgat gccgccttga tctcttcccg gtgcagctcc
     3721 ggcaggccgt acacagcctg tttcaggagg tccggatact ggtgacaatg ctcgtaccat
     3781 ttctcgtaga gcgccggatc actgagccgg taatcggcgg aaaggtccac gacctttttg
     3841 ccgagccggc gaaaccggaa ggcagcttcc tgtgcggtga cgtgaggaag ggccaggaaa
     3901 atgaagtccg ccttgcctgc aatcacatca ggatcaagcg gctcacacag aaggtccgtc
     3961 aatcccgcta gatgggggaa gatggttgta agcggcttac ccgcggattt ttccgatgtg
     4021 actgcggtaa tcgtcgcaaa acgatggtgg atcagcagcc ggtaaagttc accgcctgta
     4081 tagccgcttg accccacgat cgctatcttt acgtttgaac tggtcatatc atcaacgaac
     4141 tgcggcagtc gacattgccg gacaaccctc gagaaggata actgtctgaa tgccgggaaa
     4201 cggtttatac gtacatgacg caggcgccca acttgatacg aaaaaaagca aaataaaagg
     4261 gaaggcatct ctaccttccc ttgtcgaaca gggcctgtaa caacgagaac acaga
//
LOCUS       DASSLD010000281         2644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1265083, whole genome shotgun sequence.
ACCESSION   DASSLD010000281 DASSLD010000000
VERSION     DASSLD010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265083"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VFK23_05070"
     CDS             <1..878
                     /locus_tag="VFK23_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET7318490.1"
                     /translation="ISAVMIPSMAVFADPAAPVETPLNEERTASVPPQLKPPTEDVAQ
                     LDETEDLPEERAFDIPMVLNDSVEKHLDYFTTRGRDKFQLYLDRSARFMPMMKDIFRE
                     KNLPEDLVYVALIESGFNPYAVSWCKATGAWQFMSGTAKLYGLKIDWWIDERKDPIKS
                     TKAAAELLKDLHDQFGSWPLALASYNAGAGKVQRAVLKTRSDDFWDLKASRFIRAETK
                     NYIPKYMAATIIAKNPEGYGFTPGDHKPFKYDEVTLEESVDLRLVARCAECTYEEIKE
                     LNPELKRWVTPPPLR"
     gene            913..1320
                     /locus_tag="VFK23_05075"
     CDS             913..1320
                     /locus_tag="VFK23_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7318491.1"
                     /translation="MFLANFSAVPPEQKIKWERHEVKKGETLAGLAKKYNTSAEAIRD
                     VNGLRKNRLSPGKHLLIPVEMNETADAGYQNPGGESRQQEILYRVRRGETLAKIARKH
                     DVSVADIKEWNKGIGNSLRTGQKIKLIVDMGQI"
     gene            complement(1427..2152)
                     /locus_tag="VFK23_05080"
     CDS             complement(1427..2152)
                     /locus_tag="VFK23_05080"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7318492.1"
                     /translation="MPTIKKRGYAAPKQPEQEIITIAHIVSDFLAKYRKQFTVAVSVL
                     AVIIVLWAGFALMRSQREQKAGPLVAAAYEVYSPANGASGDYGKALELYRDIQKKYSG
                     TKSAAIAQYYIGNCLMNLGRPDEAIKEYQAFIGKYAGEKLLSGLVYQRMAYAYQTLGK
                     LEDAKKAFEQAESLLGPGVSTVELARLYEAAGNKPEADRKYKVVADKLVGTSWAMDAM
                     GKVQTQPTTPQPMAVPSSPKEAK"
     gene            complement(2339..>2644)
                     /locus_tag="VFK23_05085"
     CDS             complement(2339..>2644)
                     /locus_tag="VFK23_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HET7318493.1"
                     /translation="ILAHPGLISRDEAKLAAKNGVCLEITSRKGHSLSNGHVARGAIA
                     AGAALVIDTDTHEPGDLITDEFAENILLGAGLNKERIEEVFRNSRELASQASARLQK"
BASE COUNT          590 a          763 c          710 g          581 t
ORIGIN      
        1 taatcagcgc tgtcatgata ccttccatgg ccgtgttcgc cgacccggcc gcgccggttg
       61 aaacaccgct gaatgaagaa cgcaccgctt ctgtaccccc ccagctaaaa ccgccgacgg
      121 aagatgttgc gcagttggat gaaaccgaag acctgcccga ggaaagggca ttcgacattc
      181 ccatggtcct gaatgacagc gttgagaaac atctggacta cttcaccact cggggaaggg
      241 acaaattcca gctgtatctg gatcgctccg ctcgttttat gccgatgatg aaagacatct
      301 tccgcgagaa gaacctgccc gaggacttgg tctatgtcgc actgatcgaa agcggtttca
      361 acccgtatgc ggtctcctgg tgcaaagcga ccggcgcctg gcagttcatg tccggtacag
      421 ccaaactcta cggtctcaag atcgattggt ggattgacga acgtaaggac cccatcaaat
      481 cgacgaaggc cgccgccgaa cttctgaagg acctgcacga ccaattcggt tcctggccgc
      541 tcgccctcgc ctcctacaat gcaggggcgg gcaaagtaca gcgggcggtg ctcaagaccc
      601 ggtccgacga cttctgggac ctcaaggcct cgcgcttcat cagagcggaa acgaagaact
      661 atatccccaa atacatggct gcaacgatca tcgccaagaa cccggaagga tacggtttta
      721 ctccgggaga ccacaagcct ttcaaatacg atgaagtgac attagaggaa agcgtggacc
      781 tccgcctcgt cgctcgctgc gcggagtgca cctatgaaga aatcaaggaa ttaaaccccg
      841 agctcaagcg ttgggtcacc ccccccccac tacgataggt atgtactcag gattccgccg
      901 ggaaagaagg atgtgtttct ggcaaacttc tccgctgtcc cgcccgagca gaagatcaaa
      961 tgggagcgcc atgaagtgaa gaagggcgaa accctggccg ggctggcgaa gaagtacaac
     1021 acctccgctg aggcaatccg cgatgtcaac gggctccgta aaaacaggct ctcgcccggc
     1081 aagcacctcc tcattcccgt cgaaatgaac gagacggcgg acgcgggtta ccaaaacccc
     1141 ggcggggaga gcaggcagca ggagatcctc tatcgcgtcc ggcgcggcga gaccctcgcc
     1201 aagatcgcca gaaaacacga tgtcagcgtt gccgatatca aggaatggaa caagggtatt
     1261 ggaaattcgc tacggacagg ccagaaaata aagctgatcg tcgatatggg ccagatatga
     1321 ccgttattgc acgctgacag ggccggggat acagattccc cggccttttt tgtttgtaga
     1381 aaaccttcag gcgcgccctg caggactccc gcaatccggc agtcctttat ttcgcttctt
     1441 tgggggacga gggcacggcc ataggctgtg gggtcgtcgg ctgcgtctgc accttcccca
     1501 tcgcatccat tgcccaggat gttcccacga gtttatcagc cacaacttta tacttcctgt
     1561 cggcctcggg cttgtttccc gctgcctcgt agagtcgggc gagttctacc gtggaaacgc
     1621 ccggtccgag caaggattcg gcctgttcga acgctttctt cgcatcttcc agctttccca
     1681 gcgtctgata cgcgtaggcc attcgctggt agacgaggcc ggaaagcagc ttttcacccg
     1741 cgtatttacc gatgaaggcc tggtactcct tgatcgcttc atccggacgg ccgagattca
     1801 tgaggcagtt tccgatatag tattgcgcta ttgcagcgct cttcgtcccg gagtactttt
     1861 tctgaatgtc ccggtacaat tcgagggcct tgccgtaatc tccggacgca ccgtttgcgg
     1921 gactgtagac ttcatacgcg gctgctacga gcggtccggc cttctgctcc cgctgggaac
     1981 gcatgagcgc gaaacccgcc cagagcacga ttatgacggc aagtaccgac acggcgaccg
     2041 taaactgctt ccggtatttc gccagaaagt cggagacgat atgcgcgatg gtaatgatct
     2101 cctgctccgg ctgtttgggc gctgcatagc ctctcttctt gatcgttggc atcagtacat
     2161 cctccccttc gaaaatataa aaaagtatct atttggtcgt cattctcgcc cctgcgttaa
     2221 tcatgctggg gcaggcttca tcgggaatcc attggctttt ctaaatgctg aagttcaaaa
     2281 tggattcccg ttagaaccat tcggaatgac aaattatttt tcaagacaat caggatggct
     2341 acttttgcag ccttgcactt gcctgggaag ccagttcccg ggagttccgg aacacctctt
     2401 cgatccgttc cttgttcagc ccggcgccca gaagtatgtt ctccgcgaat tcatcggtga
     2461 tcagatcgcc gggttcatgg gtgtcggtat cgattacgag tgcagctcct gctgcgattg
     2521 cgccccgggc cacgtgtccg ttcgagaggc tgtgaccctt tcgcgaggtt atctccaggc
     2581 aaactccgtt tttcgcggca agtttcgcct cgtccctcga aatgagccct ggatgggcaa
     2641 ggat
//
LOCUS       DASSLD010000282         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_623842,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000282 DASSLD010000000
VERSION     DASSLD010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623842"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFK23_05090"
     CDS             complement(<1..225)
                     /locus_tag="VFK23_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET7318494.1"
                     /translation="MVQNASSALTDMMNRAIAREIQVSVQYMWQHVMAKGLDSAEISD
                     VFEDVSIEEMKHAEKIAERLFYFGVVPTTKP"
     gene            552..1040
                     /locus_tag="VFK23_05095"
     CDS             552..1040
                     /locus_tag="VFK23_05095"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7318495.1"
                     /translation="MSETKAIIETKFGNITLKFFPDVAPGHVKNFIDLAKKGFYDGTT
                     FHRVIPGFMIQGGDPNSKNPDKRTHGTGGPGYTIKAEFNSKPHKRGTLSMARAASPDS
                     AGSQFFICVKDSPFLDRQYTVFGEVVSGMEVVDKIVSQPRDAADNPNDRIEMKVKIEE
                     TK"
     gene            1161..>2312
                     /locus_tag="VFK23_05100"
     CDS             1161..>2312
                     /locus_tag="VFK23_05100"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET7318496.1"
                     /translation="MRETALQVRLLNKNIKKIVVAYSGGLDTSVIIMWLKETYGCEVI
                     AFTADIGQGEELEPLREKAVRTGASKIYIDDLREEFVRDFVFPLLRANAVYEGRYLLG
                     TSIARPLIAKRQIEIAVREGAEAVCHGATGKGNDQVRFELTYLALKPDVKIVAPWREW
                     TMKGREELIAYAAKHGIPVTATVSKPFSTDRNLLHISYEGGILEDPWREPTGDMYTMA
                     VPVEKAPDKSEYVEVDYERGNPVAINGKALSPAALLAEANRIAGAHGVGRVDLVENRY
                     VGMKSRGVYETPGGTLLHAAHRAVESITMDREAMHLRDGLMPRYAELVYYGYWFAPER
                     SMLQAMVDESQKNVAGTARIRLYKGNCDTVGRKSAVSLYNPELATFEADA"
BASE COUNT          500 a          718 c          673 g          421 t
ORIGIN      
        1 gggcttggtc gtggggacga cgccgaaata gaaaagccgc tccgcgatct tctccgcgtg
       61 tttcatctcc tcaatggaga cgtcctcgaa cacgtccgaa atctcggccg agtccagccc
      121 ttttgccatg acgtgctgcc acatgtactg gacgctcacc tggatctcgc gggcaatcgc
      181 ccggttcatc atatcagtca acgcgctgct tgcattctgt accatgaccc tgttctcctt
      241 tcgtgatgcg acgttcgttt cggagcgata gaaaaataat atcagaaaac aggagaaatg
      301 ggaagcgacg aatccggacc ccggagctcc gcaagcctcg ggtttcgttt gacaaccccc
      361 cggctttctg gtaaaaatgt atatctttga cctttgatgc gcattccatg ccggcttccg
      421 gcacaggagg ttgtcgattg atgaaaaaag cggctctcgt tctcgtctgg ctgtcggtat
      481 gcgtgctctt cacctcggcg tcgttcgccc agcagcagcc gaaaaaattt accccacagg
      541 agatcaaaaa aatgtcggaa accaaggcaa tcatcgaaac gaaattcggc aatatcacgc
      601 tcaagttctt ccccgacgtg gccccggggc acgtcaagaa cttcatcgac cttgcaaaga
      661 agggctttta cgacgggacc acgttccacc gcgtgatccc cgggttcatg atccagggcg
      721 gcgacccgaa ttcgaaaaat cccgacaaac ggacgcacgg caccggcggc cccggatata
      781 ccatcaaggc cgagttcaac agcaagccgc acaagcgcgg cacgctctcc atggcgaggg
      841 cggccagccc cgacagcgcc gggtcccagt tcttcatctg cgtgaaggac tcgccgtttc
      901 tcgaccggca gtacaccgtc ttcggcgagg tcgtgagcgg gatggaggtc gtggacaaga
      961 tcgtgtccca gccccgcgac gcggccgaca acccgaacga ccgcatcgag atgaaggtga
     1021 agatcgagga aaccaagtaa aaacctttct ctcgcaatga cgcagagtgc gcaaaggaaa
     1081 tctttaaatc ctggttttaa cccagggatc tgcatttctt tgcgtgctta gcgggctcag
     1141 tgtggccctc ttcttggcca ttgcgcgaga ccgctttaca ggttcgttta ttgaataaaa
     1201 acatcaaaaa aatcgtggtg gcctactccg gcgggctcga cacgtcggtc atcatcatgt
     1261 ggcttaagga aacgtacggc tgcgaggtga tcgcctttac cgcggacatc ggccagggcg
     1321 aagaactgga gccgctgcgc gaaaaggccg tcaggaccgg agcgagcaag atctacatcg
     1381 acgacctgcg cgaggagttc gtccgcgact tcgtgttccc gctgctgcgc gcgaacgccg
     1441 tctatgaggg ccgctacctt ctgggcacct caatcgcccg tccgctcatc gccaagcggc
     1501 agatcgagat cgcggtccgg gaaggcgccg aagctgtctg tcacggcgcc acgggcaagg
     1561 ggaacgacca ggtccggttc gagctcacgt accttgcgct caagcccgac gtgaagatcg
     1621 tcgccccctg gcgcgagtgg accatgaagg gccgcgagga gctcatcgcc tacgccgcga
     1681 aacacgggat cccggtgacg gcgacggttt cgaaaccctt ttccacggac aggaacctcc
     1741 tgcacatcag ctacgagggc ggcatcctgg aagacccctg gcgggaaccg acgggcgaca
     1801 tgtacacgat ggcggttccc gtcgagaagg cgccggacaa gtccgaatat gtcgaggtgg
     1861 attacgagcg gggaaacccc gtcgccatca acggaaaagc gctttccccg gcggcgctcc
     1921 ttgccgaggc gaaccggatc gccggcgccc acggcgtcgg ccgcgttgac ctcgtggaaa
     1981 accgctacgt cggcatgaag tcccgcggcg tctatgagac gccgggcggc acgctcctcc
     2041 acgccgcgca ccgtgccgtc gaatccatca ccatggaccg cgaggccatg cacctgcggg
     2101 acggcctcat gccgcgctac gcggaactgg tctattacgg ctactggttc gcacccgagc
     2161 gctccatgct ccaggcgatg gtggacgaat cgcagaagaa cgtcgcgggc acggcgcgta
     2221 tcaggctgta caagggaaac tgcgacaccg tgggccgcaa gtccgccgtg tcgctctaca
     2281 accccgaact cgccaccttc gaggcggacg cg
//
LOCUS       DASSLD010000283         3392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_300725,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000283 DASSLD010000000
VERSION     DASSLD010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300725"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFK23_05105"
     CDS             complement(<1..324)
                     /locus_tag="VFK23_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HET7318497.1"
                     /translation="MSENINIGGQAVLEGVMMRSPHAFTVAVRKGGRPGAEIAVLRET
                     IQPLSERYPLLKKKIIRGSAALFEALWLGIRALNFSANEAMEDEDGKKEEISPLAMAG
                     TMIAAL"
     gene            complement(481..681)
                     /gene="rpmE"
                     /locus_tag="VFK23_05110"
     CDS             complement(481..681)
                     /gene="rpmE"
                     /locus_tag="VFK23_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HET7318498.1"
                     /translation="MKEGIHPDYKVTTIHCACGEAFQIRSTKENVKLDICSNCHPFYT
                     GKQKLIDTEGRVDKFRKKYKKA"
     gene            complement(882..2129)
                     /gene="rho"
                     /locus_tag="VFK23_05115"
     CDS             complement(882..2129)
                     /gene="rho"
                     /locus_tag="VFK23_05115"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET7318499.1"
                     /translation="MNLVELKGKNIAELTTMADELKVEGVSGMRKQELIFAILQAQTE
                     KNGMIFGEGVLEILPDGFGFLRAPDYNYLPGPDDIYVSPSQIRRFNIRTGDTVSGQIR
                     PPKEGERYFALLKVDAINYEDPEISRSKILFDNLTPYYPIKKIKLEHSPTDYTTRAMD
                     LVTPIGMGQRGMIVAPPRTGKTMLLQAIAKAILKNHPDIKLIVLLIDERPEEVTDWQR
                     QVKAEIISSTFDEPPQRHVQVADMVSERAKRLVEHKKDVVILLDSVTRLARAHNAVIP
                     ASGKVLSGGLDANALQRPKRFFGAARSIEDGGSLTILATALVDTGSRMDDVIFEEFKG
                     TGNMELHLDRKLVDKRVFPAIDINASGTRKEELLVDKDDLNRMWILRKVLNPLSTVES
                     MEFLLEKLKQAKTNRDFLDSMNK"
     gene            2841..3293
                     /locus_tag="VFK23_05120"
     CDS             2841..3293
                     /locus_tag="VFK23_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HET7318500.1"
                     /translation="MIEGRREFVKKSVYGVLALLGLAFLIPGIKLLTPVGREEKETVF
                     FPLIAEDDLPRAGVKKAELLFSVSGKERKTRVFIVPAPEGLAVFSATCSHLGCLVNYH
                     KEKQEFVCPCHGGRYDLSGKNIGGPPPAPLTRFPVKRREGMIMVGVKV"
BASE COUNT          648 a          897 c         1002 g          845 t
ORIGIN      
        1 gagagccgcg atcatggtcc ctgccatcgc aagggggctg atctcctctt tctttccgtc
       61 ttcgtcctcc atcgcttcgt tggccgagaa gttgagcgcc cggatgccga gccacagggc
      121 ctcgaaaagg gcggccgagc cgcggatgat ctttttcttg agcagtggat accgttcgct
      181 gagcggctgg atcgtctccc gaaggactgc gatctccgcc ccgggtctgc cccctttgcg
      241 cacggcgacg gtaaaggcgt gcggtgagcg catcatgacg ccctcgagca ccgcctgtcc
      301 gcctatgttt atgttttctg acaagacagg aacctcatca atgaggctgg attcccgata
      361 ggatcattcg ggaatgacag atttgcaatt ccgcaatccg ccacataatg cccctcacct
      421 tcgtccgctc ccctcagggg agagggtggg gtgaggggtg ggccgaattc cgtattcggt
      481 ctacgctttc ttgtattttt tccggaattt gtcgacgcgg ccttcggtat caatgagctt
      541 ctgttttccg gtgtagaagg ggtgacagtt cgagcagatg tcgagcttca cgttctcctt
      601 ggtggaccgg atctggaacg cttcaccgca tgcgcagtga atcgtcgtca ccttgtagtc
      661 gggatggata ccctctttca ttgtcctcat ctcctcttcg tagaaaagcc gagccacagc
      721 ggtaggctgt ggctctggcg ttcaaggccg aaataatata ccaaaccgtg caaaaagaca
      781 agaaaaaaat ggccccgatg ctggctgcta gatcctggat gccggatgaa tgctttgttt
      841 ccagacatca agcatccggt atccagaatc cagtatcggt cctatttgtt catcgagtcc
      901 aggaagtccc gattggtctt ggcttgtttg agcttttcga gcaggaactc catgctttcg
      961 acggtggaca gcgggttcag gaccttccgg aggatccaca tgcgattcag gtcatccttg
     1021 tccacgagga gttcttcctt gcgggtgccg gaggcattga tgtcgatggc cgggaacacc
     1081 ctcttgtcca cgagctttcg gtcgaggtgg agttccatgt tgcccgtgcc cttgaattcc
     1141 tcgaaaatca cgtcgtccat gcggctgccg gtgtccacaa gcgcggttgc aaggatcgtc
     1201 aggcttccgc cgtcctcgat gctgcgggcg gctccgaaga accgcttcgg ccgctggagc
     1261 gcgttggcgt ccaggccgcc ggagagcacc ttgccggagg cggggatgac ggcgttgtgc
     1321 gcccgggcca ggcgggtcac gctgtccagc aggatcacga cgtccttctt gtgctcgacc
     1381 agccgctttg cccgctcgct caccatgtcg gccacctgca cgtgcctctg cggcggttcg
     1441 tcgaaggtgg aactgatgat ctcggccttc acctgtcgct gccagtccgt gacctcctcg
     1501 ggccgctcgt cgatcaggag cacgataagc ttgatgtcgg gatggttctt caggatcgcc
     1561 ttggcaatgg cctgaagcag catggtcttg ccggtccggg gaggcgcaac gatcatgccg
     1621 cgctggccca tgccgatggg ggtaacgagg tccatggcgc gggtcgtata atccgtcgga
     1681 gaatgttcga gtttgatttt tttgatcggg taatacggcg tgaggttgtc gaagaggatc
     1741 ttgctgcgcg atatctcggg gtcttcgtag ttgatcgcgt cgaccttgag aagggcgaag
     1801 tatcgctccc cctctttcgg cggtcggatc tggccggaga ccgtatcgcc tgtccggatg
     1861 ttgaaccgcc gtatctgaga cggagaaaca tagatgtcgt ccgggccggg caggtaattg
     1921 tagtccgggg cccggaggaa gccgaagccg tccggaagga tctcgaggac gccctcgccg
     1981 aagatcatgc cgttcttttc cgtctgggcc tggaggatcg cgaagatcaa ctcctgtttg
     2041 cgcatgccgc tgacgccctc gactttcagc tcgtccgcca tggtggtaag ttcggcgatg
     2101 tttttccctt tgagttctac taagttcatg tgactactcc ttagattccc ggccttatag
     2161 gtcagatgag gtctgtgtat gtggttaaaa cgagttgtgg tttatgggtg atcatgcagt
     2221 cgagagaacg ggtacatcgt caatcaatgt tcatggaatt tactgcaaca gtgtttgcca
     2281 ttgccgcagt ctctaccggg ggtacggcgc gtttggaccg aggggaccgt gttcccgcgc
     2341 cttctgttgc ccgacatgat ttgatccaag aggattacgc cggcgcaccc tgctcttgcg
     2401 cagtcgcatt cgaaaacgtc gtgtgcagtc attcagctaa tctaaaaaaa cccggattcc
     2461 cgcccggaac gctacgggaa tgaaaaagag gtcatttgcc agggcctcga tggtaattat
     2521 catgaataag gggagtgttg tctcgggtcg tcggtttgcg tggttttcgt gataatggtg
     2581 atgttacgta aacgacggct gccagttgcg tgtgtgctcc ggatgatacc ttcctgtttg
     2641 tatgctatct tctctggtta attacctgat tgtgtctcgt gtaatgagtt gggtttggta
     2701 atacctggga ttgtcggcta ggcctgcgct ttggtgatgt tctttggcaa taaaaatact
     2761 gtaccgcaga aaaggctatt tgtcaacaga ttattttttt taccgttgac atgtctcttg
     2821 cgacagtagt ataaaaactc atgatagagg gccgccggga attcgtcaag aagtcggttt
     2881 acggagtgct cgcgctgctc ggactcgcct tcctcattcc cggcatcaaa ttacttacgc
     2941 ccgtcggcag ggaagagaag gagacggtct ttttcccgct cattgccgag gacgacctcc
     3001 cccgcgcggg cgtgaagaag gcggagcttc tcttctcggt ttccggcaag gaaagaaaga
     3061 cccgggtctt catcgtcccg gctcccgaag ggctggcagt gttctccgcc acctgctcac
     3121 acctcggctg ccttgtcaac taccataaag agaagcagga attcgtctgt ccctgccacg
     3181 gcggtagata cgacctgtcc ggaaaaaaca tcggcggtcc gccgcctgcg ccgctcacgc
     3241 ggttcccggt caaaagacgg gaaggcatga tcatggtggg ggttaaagta tagttcggag
     3301 ttgggagttc ggagtaaaga ctatctgtta tgaaaatttt caactatctt gatacgcgga
     3361 tggggtttac aagccggcac gggagggtgc tg
//
LOCUS       DASSLD010000284         3787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_70793,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000284 DASSLD010000000
VERSION     DASSLD010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70793"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..910
                     /gene="argF"
                     /locus_tag="VFK23_05125"
     CDS             2..910
                     /gene="argF"
                     /locus_tag="VFK23_05125"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007961601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET7318501.1"
                     /translation="MLAVSDLAPKDIDNILKRAAVLKKMLRQGKARPTLKGKTLGMIF
                     EKSSTRTRVSFEVGMYQLGGLALFLSSADLQIGRGEPIADTARTLSRYVDGIMIRTFS
                     QSTVKELARHATVPVINGLSDLHHPCQALADLLTIREKKGKLKGLKLAYVGDGNNVAN
                     SLIEACVKTGMHISIACPHGYEPDAAVMRDATREAARTGSELRIVHAPLKAVKDADAI
                     YTDVWASMGQEAEHAVRVRAFRGYQIDAKLMKAARPGAFAMHCLPAHRGEEISAEVID
                     GPRSVVFDQAENRLHAQKAVLELLMK"
     gene            1089..1796
                     /locus_tag="VFK23_05130"
     CDS             1089..1796
                     /locus_tag="VFK23_05130"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET7318502.1"
                     /translation="MKPLIGITMNMEVQPSRTINVLDQDYGKVVVKAGGIPVPVLGIE
                     GSVPDLIRRLDGFIFTGGDDIDPRFYKEKPLAGARMTFSPDGRTRFEIGLFKAAAKAK
                     KPLLAICYGAQLVNVALGGRLYQDISQQVPHPIKHGPARAGERVFHDVDILEGTMLSR
                     ILGTPRIRVRSAHHQSVKNPGRGLHLSAVARDRVIEALEPRTNQFLIAVQWHPEKTPN
                     DRYSRKLFEALISAAKK"
     gene            1741..3408
                     /locus_tag="VFK23_05135"
     CDS             1741..3408
                     /locus_tag="VFK23_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7318503.1"
                     /translation="MTDIPGSSSRPSSPLRKNSPGPGAGKRLPLLLLLLFLAALLILA
                     SAVGCHRTDEGRLPGYLYLRLNNNPTTLDPALITDVQGGGIAAKLFNGLVRFSENLDI
                     VPDLARSWQLSADQMTYTFRLRPDVLFSNGRRVTARDVKYSFERVLIPQTKAPLTWVL
                     DKIEGANDVIAGKTTDLTGVRVVNDYTLTIRLVKPFGPFLSLLAMTTAYVVPREDVER
                     LGGDFGTRPVGSGPYTLHEWKHGQHVVLAAREDYFEGRPKLNGIYYRIIPEDLTAVME
                     FETGRLDVLLIPSSEYRRYTTDPAWRDLVHGRPGLNSYYLGLNCTHPPFNDIRVRHAV
                     NYAIDRLHILNTVFEKRGVLATGPVPPGLWKRHILPRALKGYPYDPDKAKELIRQAGA
                     EGATVNIYITADPEVLDIVEVIQGYLAKVGLKAKITQLDWSAFKQAVNEGEPDAFWLS
                     WWADYPDPENFLYPLFHSASVGSAGNRTRCLDPEVDRLIDKAQHTMDERQRYRLYRQA
                     EDRIVQNAPWVFMWHRADYFVVQPWVQDFKIYPIYSIDKRVDVTVKR"
     gene            complement(3477..>3787)
                     /locus_tag="VFK23_05140"
     CDS             complement(3477..>3787)
                     /locus_tag="VFK23_05140"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HET7318504.1"
                     /translation="VVAIHLRDVHNVPSLTQEMVGNIKQAFSKAEPIAVPPEQDKLVL
                     KEVSCKDLGMNCGFRYIAQTEELIADGVAVHAREAHGINVFTAEMATKVKNLAHPWKG
                     "
BASE COUNT          795 a         1197 c         1058 g          737 t
ORIGIN      
        1 cctgctcgcc gtttcggacc ttgcgccgaa agacatcgac aacatcctga aacgcgccgc
       61 agtcctgaaa aaaatgctgc gtcagggcaa ggcccggccg accctgaagg ggaagaccct
      121 cggcatgatc ttcgaaaagt cttcgacgag gacgcgggtt tccttcgaag ttggcatgta
      181 ccagctcggt ggcctggccc tcttcctgtc gtccgccgac ctccagatcg gccgcggaga
      241 accgatcgcc gatacggcgc gcactctctc ccggtacgtc gacgggatca tgatccgcac
      301 attttcccaa tctacggtca aggaactggc gcgccatgcg acggttcccg tgatcaacgg
      361 cctgtccgac ctccaccacc cctgccaggc ccttgcggac ctcctcacca tccgggaaaa
      421 aaagggaaaa ctgaaggggc tgaaactcgc ctacgtgggc gacgggaaca atgtggcgaa
      481 ttccctcatc gaggcctgcg tcaagaccgg gatgcatatc tccatcgcct gccctcacgg
      541 ctacgaaccc gatgcggccg tgatgcggga cgccacgcgc gaggccgcgc ggacgggctc
      601 ggaactcagg atcgttcatg ctcccctcaa ggcggtgaag gacgcggacg cgatctacac
      661 cgacgtctgg gcgagtatgg gccaggaggc ggagcatgcc gttcgcgtgc gggcgttcag
      721 gggataccag atcgacgcga agctcatgaa ggcggcacgg cccggggcgt tcgcgatgca
      781 ctgccttccg gcgcaccgcg gggaggagat ttctgccgag gtgatcgacg gcccgcggtc
      841 cgtcgtcttc gaccaggccg agaaccgcct ccatgcgcag aaggctgtgc tggagctgct
      901 gatgaaatag gatcggatgc cgatttccgc tgttttaacc gcattcatcc ggtaaaatta
      961 taagaagaaa taaagacaac tgacttgacg caatagttct caaattacaa tctcacgctg
     1021 acaatcctcc ggaaatgtgc tatatatgac gaagtctgca cccgcactct atttccggag
     1081 gtgaacgcat gaaacctctc atcgggatca cgatgaacat ggaggtccag ccctcacgga
     1141 ccatcaacgt tctggaccag gactacggaa aggtagtcgt gaaggcgggc ggcattccgg
     1201 tgccggtcct cggcatcgag ggctccgtcc ccgatctgat caggcgtctt gacggtttca
     1261 tctttaccgg aggcgacgac atcgatcccc ggttctacaa ggagaaaccg cttgccggtg
     1321 cgcgcatgac gttctctccc gacgggcgga cccggttcga gatcgggctt ttcaaggcag
     1381 cggcgaaagc caaaaaaccg ctcctcgcca tctgctacgg cgcgcagctc gtcaatgtag
     1441 cgctcggcgg tagactgtat caggacatca gccagcaggt ccctcacccg ataaagcacg
     1501 gccccgcccg cgccggggag agggtgtttc acgacgtcga tatcctggaa ggcacaatgc
     1561 tctcgcgcat ccttggcacg ccccgcatcc gcgtgcggag cgcacaccat cagagcgtga
     1621 aaaaccccgg ccgggggctc catctctccg ccgtcgcgcg cgaccgggtt atcgaagccc
     1681 ttgagcccag gacaaaccag tttctgatcg ccgtacaatg gcatccggaa aagacaccca
     1741 atgaccgata ttccaggaag ctcttcgagg ccctcatctc cgctgcgaaa aaatagcccc
     1801 gggccggggg ccgggaagcg gctgccgctc ctcctgctcc ttctgttcct cgccgcgctc
     1861 ctcatccttg cctccgctgt cggctgccac aggaccgacg agggacgtct ccccggctat
     1921 ctctacctca ggttgaacaa taatccgacc accctcgacc cggcgctcat aaccgatgtc
     1981 caggggggag gcatcgcggc gaagctcttc aatggactgg tccgtttcag tgaaaacctc
     2041 gacatcgttc ccgacctcgc ccggtcgtgg caattgtccg ccgaccagat gacctacacg
     2101 ttccggctcc gccctgacgt tctcttcagc aacggcagga gggtcacggc ccgggacgtc
     2161 aagtattcct tcgagcgcgt actcatcccg cagacaaagg ctcccctcac ctgggtcctc
     2221 gacaaaattg aaggcgcaaa cgacgttatt gccggaaaaa cgacggacct caccggcgtg
     2281 cgcgtggtaa acgactatac ccttaccatc aggctcgtca agcccttcgg gccgttcctg
     2341 tcgctgcttg ccatgaccac ggcctacgtc gtgccccggg aggatgtcga gcgcctcggc
     2401 ggggatttcg gcacgagacc cgtgggcagc ggtccctata ctctgcatga atggaaacac
     2461 ggacagcacg tggttctcgc cgctcgggaa gactacttcg agggacgtcc caaactgaac
     2521 ggcatttact accgcatcat tcccgaggac ctgacggcgg tgatggaatt cgagaccggc
     2581 cggctcgacg tactcctcat cccttcttcc gagtaccggc gctatactac ggatcccgcc
     2641 tggcgggatc tcgtacacgg caggccggga ctgaacagct attacctcgg cctcaactgc
     2701 acccatcccc ccttcaatga catcagggtc cgtcacgcag tcaattatgc catagaccgg
     2761 ctgcacatcc tgaacaccgt ttttgaaaag cgcggcgttc tggcaaccgg tcccgttcct
     2821 cccgggcttt ggaaacgcca catccttcct cgcgccttga aagggtatcc ttacgacccg
     2881 gataaggcga aggaactgat ccgtcaggcc ggcgccgaag gggcgaccgt caatatctac
     2941 attactgcgg accctgaggt tcttgacatc gtcgaagtga tccagggcta ccttgcgaag
     3001 gtgggtttaa aggcgaagat cacacaactg gattggagcg ccttcaagca ggcggtaaac
     3061 gaaggagagc ctgatgcctt ctggctctcc tggtgggcgg actacccgga cccggaaaac
     3121 tttctgtacc cgctctttca ttcggcgagc gtgggttcgg ccggtaacag gacaaggtgc
     3181 ctcgacccgg aagtcgaccg tctgatagac aaggcccagc acactatgga cgagcgtcaa
     3241 cggtaccgcc tctaccggca ggccgaggac cgaatcgtcc agaacgctcc gtgggtattc
     3301 atgtggcacc gggccgacta cttcgtggtt cagccctggg tacaggactt caagatctat
     3361 cccatctatt ccatcgacaa gcgggtcgat gtaacggtaa aacgataaaa aaagccgcgc
     3421 ctacggcgtg gctgcaatga actttgcact ttgtactccg aactctgtac tttattctag
     3481 cccttccacg gatgggcgag atttttgacc ttcgtggcca tctcggctgt aaatacgttg
     3541 atgccatggg cctcccttgc atgaaccgcc acgccgtcag cgataagctc ctcggtctgg
     3601 gcaatgtacc tgaatccgca attcattccg aggtccttgc acgatacctc cttgaggacg
     3661 agtttatcct gttccggagg aaccgcaatg ggttcggcct tgctgaatgc ctgcttgatg
     3721 ttgcccacca tttcctgcgt caatgacgga acattatgca cgtcccgcag gtgaatggcg
     3781 acaacgt
//
LOCUS       DASSLD010000285         4187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_330176,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000285 DASSLD010000000
VERSION     DASSLD010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330176"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFK23_05145"
     CDS             <1..251
                     /locus_tag="VFK23_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     cytochrome b subunit"
                     /protein_id="HET7318505.1"
                     /translation="RPDVFLMVVRSFEQIGISGVYLIALAALGLHLLHGIQSSFQTWG
                     LNNERTLPVIERTGFVASVVLFIWYAAIPVSILMKIFK"
     gene            353..2266
                     /locus_tag="VFK23_05150"
     CDS             353..2266
                     /locus_tag="VFK23_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HET7318506.1"
                     /translation="MILDGKIPSGPLPEKWDKRRAEMKLVNPSNKRKYRIIVVGTGLA
                     GASAAASLGELGYNIDAFCFQDSPRRAHSIAAQGGINAAKNYPNDGDSIERLFYDTIK
                     GGDFRSREANVYRLAQVSANIIDQCVAQGVPFARDYGGYLATRSFGGAQVSRTFYARG
                     QTGQQLLLGAYGALSRQVKVGAVTLHPRTEMLDLVLVNGEAKGITVRDLVTGTISSYA
                     ADAVCLATGGYTNVFFLSTNAQGNNVTAAFRAYKRGAFFANPCFTQIHPTCIPVTGEH
                     QAKLTLMSESLRNDGRVWVPKKKGDVRPAAEIPENERDYYLERKYPAYGNLAPRDISS
                     RAAKEVCDEGRGVGPGGRGVYLDFSDAIKRLGEPVIRERYGNLFDMYERITAENAYRQ
                     PMRMYPAPHYAMGGLWVDYDLQSNIPGLFVLGEANFSDHGANRLGASALMQGLADGYF
                     VIPYTISNYLAQTKPGAVTASHPEFIKSWEDAEAFTKKLLAIKGKKTVHELHRELGKI
                     MWDYVGMTRSKESLKTALKQIPELRAEFWENVVIPGNETDLNQELERAGRLADFLEFA
                     ELLTRDALDRDESCGAHFRVEHQTPDGEAVRNDKDFSYVAAWGYKGPDKEPELHKEPL
                     LFEYAHLAVRSYK"
     gene            2410..3210
                     /locus_tag="VFK23_05155"
     CDS             2410..3210
                     /locus_tag="VFK23_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HET7318507.1"
                     /translation="MNLTLYVWRQKGPSDKGRLVQYEATDVSSDMSFLEMLDTVNESL
                     IKKGEEPIAFDSDCREGICGMCGQVVNGVPHGPRKKTTVCQLHMRHFKDGDTIYIEPF
                     RARAFPVIKDLVVDRGAFDRIIQAGGYVSVHTGGVPDGNAILVPKRDADIAMDAAECI
                     GCGACVAACPNGSAMLFTSAKVSHLALLPQGRVEAARRVCVMTEEMLGAGFGNCSNHY
                     ECESVCPKGIRVKFIAKLNREFIKAQAPGSGCRDGAAEPGTAPADEED"
     gene            3331..>4187
                     /locus_tag="VFK23_05160"
     CDS             3331..>4187
                     /locus_tag="VFK23_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7318508.1"
                     /translation="MKRFMYVLALALMVSLFAGYVHAADTFDLTIPLPLTGKPAKFGE
                     IMKRSYEMAAEEINAKGGVKGKKIVLSFEDSSGKPETARAIVEKLIDSKKQPIVVGEY
                     TSACAKAVAAVAEERKTPYLVVASADDDITQKNYKYVFRQNQVNAHYGDAVVSFLKEV
                     VKPKTIAILHESTAFGTSGAEAMEKDAAKIGAKVVIKEKFETGAIDFRPLLSKVKAAQ
                     PDVVYTVAYANEAQLLMKQAKEMRVDAKLYSGGAAGFAIPEFIKGAQDAAEYVVSATL
                     WTPQLKYPGA"
BASE COUNT          925 a         1275 c         1228 g          759 t
ORIGIN      
        1 gcaggcctga tgtcttcctg atggttgtgc gaagcttcga gcagatcggc atttccggtg
       61 tctatctcat tgcgctcgcg gctctggggc ttcacctcct ccacggcatc cagagctcgt
      121 tccagacctg gggattgaac aatgagagaa cacttccggt gatcgaaaga acagggttcg
      181 ttgcatcggt cgttctgttc atctggtatg cagcgatacc ggttagcatt ttaatgaaaa
      241 tatttaaatg aaattcgaag cacgctctgc taaatatgaa acaataaccg aacacctaca
      301 ctcaagacga gtttaggatt tcgaaaatgg aattcggatt tgagatgaca tcatgatcct
      361 tgacggcaaa ataccttccg gtccgctccc ggagaaatgg gacaagcggc gcgccgagat
      421 gaagctcgtg aacccctcga acaagaggaa gtatcgcatc atcgtcgtcg gcacgggcct
      481 tgccggcgct tccgccgctg cctcgctcgg agagctgggc tacaacatcg acgccttctg
      541 cttccaggac tcgccccgcc gcgcccacag catagcggcc cagggcggaa tcaacgcggc
      601 caaaaactac ccgaacgacg gggacagcat cgagcgcctc ttctacgaca cgatcaaggg
      661 cggcgacttc cgctcccgcg aggcgaacgt ttaccgcctc gcccaggtga gcgccaacat
      721 catcgaccag tgcgtggcac agggcgtccc cttcgcccgc gactacggcg gatatctcgc
      781 gacccgctcc ttcggcgggg cccaggtctc gcgcaccttc tacgcgcgcg gtcagaccgg
      841 gcagcagctc cttttggggg catacggcgc tctctcgcgc caggtcaaag tgggcgcggt
      901 gaccctccat ccccgcacgg agatgctcga tctcgtcctc gtaaacgggg aggccaaggg
      961 catcacggtg cgcgacctcg tgaccggcac gatctcatcc tacgccgcgg acgccgtctg
     1021 tcttgcgacc ggcggctata ccaacgtctt cttcctctcc acgaacgccc agggcaataa
     1081 cgtgacggcc gccttccggg cgtataagag gggggcgttc ttcgccaacc cctgcttcac
     1141 ccagatccac cccacctgca tccccgtgac cggcgagcac caggccaaac tcacgctcat
     1201 gtcggagtcc ctcagaaacg acggcagggt ctgggtgccg aagaagaaag gcgatgtccg
     1261 gcctgctgcc gaaatcccgg aaaatgagcg ggattattat ctcgaacgaa aatatcccgc
     1321 gtacggcaat ctcgcgccgc gggatatctc ctcgcgcgcg gcgaaggagg tctgcgacga
     1381 aggccgtggc gtcggacccg gcggccgcgg cgtgtacctc gatttctccg atgccatcaa
     1441 gcgtctggga gaacccgtga tccgcgagcg ctacggaaac ctcttcgaca tgtatgagcg
     1501 catcaccgct gaaaacgcct accggcagcc catgcgcatg tatcccgcgc cccattacgc
     1561 catgggtggg ctctgggtgg actacgatct ccaaagcaac attcccggcc tcttcgtcct
     1621 gggggaggcg aatttttcgg accacggtgc gaaccgcctc ggggcgagcg ctctcatgca
     1681 gggccttgcc gacgggtatt tcgtgatccc ctacacgatt tccaactatc ttgcacagac
     1741 gaagccgggg gcagtgactg catcccaccc ggaattcatc aagtcatggg aagacgcgga
     1801 agccttcacc aagaaactcc ttgccatcaa ggggaaaaag accgtgcacg aactccaccg
     1861 cgaactcggc aagatcatgt gggactatgt cggcatgacc cggagcaagg agagcctgaa
     1921 gaccgcgctc aagcagatcc cggagctgcg cgccgagttc tgggaaaacg tcgtcattcc
     1981 gggaaacgaa acggatctga accaggaact cgaacgggcg ggccgccttg cggacttcct
     2041 cgagtttgcc gaactgctga cgagggacgc ccttgaccgc gacgagtcct gcggggcgca
     2101 tttccgcgtc gagcaccaga cgcccgacgg cgaagccgtc agaaacgaca aggacttcag
     2161 ctatgtggcc gcatggggat acaagggccc ggacaaggag ccggaacttc acaaggaacc
     2221 gctcctcttc gagtacgcgc atctggcggt gagaagctat aaatagaatg cggaattcgg
     2281 aatgcggagt gcggaataaa agcgtcattc ccgcccctgc ttcaatcatg ctggggcagg
     2341 ctccagcggg aatccagggt ttctggctcc cgggtcaagc cctgaatgac agtcaaggat
     2401 gatttcttta tgaatctgac cttatacgtc tggcgtcaga aagggccttc tgacaaaggt
     2461 cgcctcgtcc aatacgaggc gacggacgtg agctccgaca tgtcctttct cgagatgctc
     2521 gacaccgtga acgaaagcct tatcaagaag ggcgaagagc ccatcgcctt cgacagcgac
     2581 tgccgcgagg gcatctgcgg catgtgcggc caggtcgtga acggcgtccc ccacggcccg
     2641 aggaagaaga ccacggtctg ccagctccac atgcgtcatt tcaaggacgg tgacacgatc
     2701 tacatcgagc cgttccgcgc ccgcgctttc cccgtgatca aggacctcgt cgttgaccgg
     2761 ggtgccttcg acaggattat ccaggccggg ggctacgtct cggtccacac cggcggcgtg
     2821 ccggacggca acgcgatcct ggtccccaag cgcgacgccg atattgccat ggacgcggcc
     2881 gagtgcatcg gctgcggggc ctgcgtcgct gcctgcccga acggttccgc catgctcttt
     2941 acttccgcga aggtatcgca ccttgccctg ctcccgcaag ggcgggtcga ggcagcccgc
     3001 cgcgtctgcg tcatgaccga ggagatgctc ggtgcgggct tcggaaactg cagcaaccac
     3061 tacgaatgcg aatccgtctg ccccaagggc atcagggtga agtttatcgc gaaattgaac
     3121 cgggagttca tcaaggctca ggccccggga agcggatgca gggacggagc agcggaaccc
     3181 gggacggctc cggccgacga ggaagactga tacgattgat gatcggcgat tgtcgattgg
     3241 tgattaaaaa cgaaagaaca tcaaaacctc ctttcaatca acaatcatca atcaacaatc
     3301 atcaatgcat tctaatctaa ggagggaacg atgaaacggt tcatgtatgt tctggcactg
     3361 gcactgatgg taagtctttt cgccgggtac gtgcacgcag cggacacgtt cgatctcacg
     3421 atcccgctgc cgctcaccgg caaaccggcc aagttcggcg agatcatgaa acgttcctat
     3481 gagatggcgg cggaagagat caacgccaag ggcggtgtga agggcaagaa gattgtgctt
     3541 tcgttcgagg actcgtccgg caagcctgag acggcccgtg ccatcgtcga aaagctgatc
     3601 gattcgaaaa agcagcccat tgtcgtcggt gagtacacct ccgcctgcgc aaaggccgtc
     3661 gcagcggtgg ccgaagagcg gaagaccccc tatctcgtcg tggcgagcgc cgacgacgac
     3721 ataacccaga agaactacaa gtacgtgttc cgccagaacc aggtgaacgc ccattacggc
     3781 gacgcggtcg tctcctttct taaggaagtt gtgaagccca agaccatcgc catcctccac
     3841 gagagcacgg ccttcgggac ttcgggcgcc gaggcaatgg agaaggacgc ggcaaagatc
     3901 ggggctaagg tggtgatcaa ggagaaattc gagaccggcg ccattgattt caggcccctc
     3961 ttgtccaagg tgaaggcggc gcagcccgat gtcgtctata ccgtcgccta tgccaacgag
     4021 gcccagcttc tcatgaagca ggccaaggaa atgcgcgttg atgccaagct ctattccgga
     4081 ggcgcagcgg gctttgcgat ccctgagttc atcaagggcg cgcaggacgc ggccgagtat
     4141 gtggtaagcg ccacgctctg gaccccccag ctcaagtacc ccggagc
//
LOCUS       DASSLD010000286         4083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_390351,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000286 DASSLD010000000
VERSION     DASSLD010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390351"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFK23_05165"
     CDS             complement(<1..230)
                     /locus_tag="VFK23_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_248595823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET7318509.1"
                     /translation="MRVLRCIAVLTLFVLPIAAFSPLNAHAAETKKAKTIDELAKMYD
                     STGCKTCHEDIYKEWEQSIHSRSIFGTGRTAAT"
     gene            complement(399..1811)
                     /locus_tag="VFK23_05170"
     CDS             complement(399..1811)
                     /locus_tag="VFK23_05170"
                     /inference="COORDINATES: protein motif:HMM:NF023281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3373 family protein"
                     /protein_id="HET7318510.1"
                     /translation="MKKVFLLVISLLLLTAGFAAAADDASWLEIGGDYQYRFDSLKGT
                     VHDYYEFQDALAAQLGGTAPAITKGYDVKNDSLMTNRFGLNLRANAMEDVSVKARLIM
                     YKVWGHETSTPVNGSFFSDRATGSNDGTIGHIPQDNTLRVDYAYATWSNIGEMPIWFS
                     VGRRPTTGGIPTNIKNNIEKTGTAGVPGILVDFAFDGATLGYAPEIEALPGSFVKLCY
                     GKGFDSGFVTTAGSSLKDVEFYGVNVAVVDSEALKAQVQYQRGAHIFDAPSDGSALTG
                     PVRHNLGDIDWVGGVVMGKVSDLNLFVSAAGSQTHPNNQMFEVPGPTGPIPVAGLLYD
                     NGSPADSKIGQAIYVGGRYDFAATGTKIGAEYNHGTKNWIGMVPAAEDRWTSKLGTRG
                     NVYEVYVIQELRNKPIAKKGQAFFKLGYQAYKFDYTGSNNWVGAPKKISDLTASPANA
                     QMLVPIEKATDIYLTFEVRF"
     gene            complement(2106..2423)
                     /locus_tag="VFK23_05175"
     CDS             complement(2106..2423)
                     /locus_tag="VFK23_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318511.1"
                     /translation="MKNETVKSGMKIGATVGGLVFLVFGIMPGFYFGSYGTLILLQKL
                     MGGTVEPTLLVRAVIVMGIVVGIACAAAVSIVVGGLLGTAMGFIVSAPASVRSRQAAT
                     EKA"
     gene            complement(2426..2740)
                     /locus_tag="VFK23_05180"
     CDS             complement(2426..2740)
                     /locus_tag="VFK23_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318512.1"
                     /translation="MKITIRRQGAYIGAGAGLVLFALFGLLPGSLLGGAAGIKIAGML
                     FGLPLEPGLLSRAIVLASMLAGVLVAGTAIVTASSTIGWLFGAAFGSSPQENTLASAE
                     RR"
     gene            complement(3000..3440)
                     /gene="cynS"
                     /locus_tag="VFK23_05185"
     CDS             complement(3000..3440)
                     /gene="cynS"
                     /locus_tag="VFK23_05185"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HET7318513.1"
                     /translation="MNTPEITERLLAAKKQKGLSFAELGKTIGRDEVWVAALLYRQAS
                     ASEEEAQKLAAALDLGPDAAEALTEFPVKGLGTVVPSDPLLYRFHEILQVYGMPLKAV
                     IHEKFGDGIMSAVDLTLTVHKEHTLKGERVRVTLSGKFLPYKKW"
     gene            complement(3627..>4083)
                     /locus_tag="VFK23_05190"
     CDS             complement(3627..>4083)
                     /locus_tag="VFK23_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HET7318514.1"
                     /translation="QAAGGDVRGRQSAALIVAGKPTGRPWADRTFDLRVDDHPEPLGE
                     LRRLLVLQRAYNHMNAGDAAMEHKNHAKALEEYGAAAGLAPDNLEIVYWHAIALVNMG
                     RIKEAIPLFRKVFARDKNWVTLTPRLAEAGLLPDDRGLTDRICGVDDEA"
BASE COUNT          814 a         1224 c         1118 g          927 t
ORIGIN      
        1 gttgccgcgg tcctgccggt gccgaagatg gaccgggagt ggatggactg ctcccactcc
       61 ttgtagatgt cttcgtgaca cgtcttgcag ccggttgagt cgtacatctt tgcaagttcg
      121 tcgatggtct tcgccttttt cgtctccgcg gcatgagcgt tcagaggaga gaacgccgca
      181 atgggcagga cgaataaggt gagtactgcg atgcatctta aaaccctcat acatttcctc
      241 ctttcatggg atgagaaccc cgtgataccg gggccgacaa caattcatga atgcccgcta
      301 ctttgtgaaa cttcccgtaa cagcccgcgg gggaagcggt tacagcgaaa tttaccggga
      361 agtatagcat agtttatttt tttcatccga ctccaatcct agaaccggac ctcaaaggtg
      421 agatagatgt ccgtcgcctt ttcgatcgga acaagcatct gggcgttcgc ggggctcgcg
      481 gtcaggtcgg agatcttttt cggagcgccg acccagttgt tgctgccggt atagtcgaac
      541 ttgtacgcct gataaccgag cttgaagaag gcctggcctt tctttgcgat gggtttgttc
      601 cggagttcct ggatcacgta gacctcatag acgtttccgc gggtcccgag cttgctcgtc
      661 caccggtcct cagcggcagg caccatgccg atccagttct tcgtcccgtg gttgtactcc
      721 gcgccaatct tcgtgccggt ggcggcaaaa tcatagcgtc caccaacgta gatcgcctgg
      781 ccgattttgc tgtcggcggg gctgccgttg tcatagagca gtccggctac ggggatgggg
      841 cccgtcggtc cgggtacctc aaacatctga ttgttcggat gggtctggct cccggcggcg
      901 gagacgaaga ggttgaggtc gctcaccttg cccatcacca cacctccgac ccagtcgatg
      961 tctccgagat tgtgcctcac ggggccggtc agcgcgctcc cgtcggaagg cgcatcgaag
     1021 atgtgcgcgc ccctctggta ctgcacctgc gccttcagtg cttctgaatc gacgacggcg
     1081 acgttcacgc cgtaaaattc gacgtccttg agcgacgagc ctgcagtcgt tacgaagccg
     1141 ctgtcaaacc ctttgccgta gcagagcttc acaaacgagc ccggcagcgc ctcgatctcc
     1201 ggcgcgtagc ccaaggttgc gccgtcgaag gcgaagtcga cgaggatgcc gggtacgccc
     1261 gctgttccgg tcttctcgat gttgttcttg atgttggtcg gaatgccgcc ggtggtgggc
     1321 cgcctgccca cggagaacca gatcggcatc tcgccgatat tgctccaggt ggcataggca
     1381 taatcgaccc gcaacgtgtt gtcctgggga atgtgtccta tcgtcccatc gttcgaaccc
     1441 gtcgccctat cggagaagaa cgacccgttg accggtgtcg aggtctcatg accccatacc
     1501 ttgtacatga taagacgtgc cttgacgctg acatcctcca tggcgttggc tctcaggttc
     1561 agtccgaacc ggttcgtcat gagggaatca ttcttgacgt cgtacccttt cgtaatggcc
     1621 ggcgcggtgc cccccagttg cgcggccagc gcatcttgaa actcatagta gtcgtgcacc
     1681 gtgcccttga gcgagtcgaa ccggtattga taatctccgc cgatttcgag ccatgaggcg
     1741 tcatcggccg ctgccgcaaa acccgcggtc agaaggagca gcgaaattac aagcaaaaaa
     1801 actttcttca ttgcgtaata cctccgatta aatttaaatg cagtaaagca gtactgttgc
     1861 cgtacttccc cgttcacccc ctttcagaaa aaggaataag tacgcagccg ttatgagcat
     1921 gcgcacatca aagtgatctc ccctgttatt gtttcgttgt ttctaacttt tgaaactata
     1981 agggctaaaa aataccgctc atggcgagcg ggtcgtaaaa acaacgggga gtaagattca
     2041 cgcttataaa ttcagggcag cctttgacgg ctgccctgaa ttttatttcg cgcttcggac
     2101 ttgacttacg ccttctctgt tgcggcctgc ctgcttctca ctgacgccgg agccgacacg
     2161 atgaacccca ttgcagtgcc caggagtccg cctacgacga tgctcaccgc ggccgcgcag
     2221 gcgataccga ccacgatccc catcacgatg accgcgcgga ccagaagggt cggctccacc
     2281 gtcccgccca tcagcttctg gagcaggatc aatgtcccgt aggacccgaa gtagaacccc
     2341 ggcatgatgc cgaacaccag aaataccagc ccccccaccg ttgccccgat cttcattccc
     2401 gattttaccg tctcgttctt catgactatc tcctttcagc cgaagccaaa gtattttcct
     2461 gcggtgatga tccaaaggcc gcgccgaaaa gccagccgat ggtggatgat gccgttacga
     2521 tcgccgtccc ggccacgagc acgcctgcga gcatggatgc gagaacgatc gcccgcgata
     2581 ggagacccgg ttccaggggc aggccgaaca acatgcccgc gatcttgatg cctgccgcgc
     2641 ctcccaggag gctgccgggc aaaagcccga agagcgcgaa cagaaccaat cccgctcccg
     2701 cgcctatata tgcaccctgt cttcttatgg tgatcttcat tttcgcgtcc tccctttcgt
     2761 tcggtcttgc ctagcgttat tgcacactgt atgccaaaag ataaggcgct gaattttcag
     2821 gaaagaggtg gtggagatgt tttgcagaag tgagaagcgg ggagtatttt ttttagagat
     2881 gcaaaaaaac gatgttatac gccacgcttt tatggtcgat catgcagctg gcagggagat
     2941 cgctccggag cgatgtcgga cgaaatccgg tccggataag cgcgtgtgca cagagcctct
     3001 taccacttct tatagggaag gaattttccg gacagggtaa cccgcaccct ctcgcctttc
     3061 agggtatgct ccttatgaac ggtcagcgtc aggtcaacgg cgctcatgat cccgtcgccg
     3121 aatttctcat ggatcacagc cttgagcggc atgccgtaga cctgcaggat ctcatggaag
     3181 cgatacagga gcgggtcgga aggcacgacg gtccccagcc ctttcacggg gaactcggtg
     3241 agtgcttcgg ctgcatccgg tccgagatcg agcgcagccg ccagtttctg cgcctcttcc
     3301 tcggaggcgc tcgcttggcg atagaggagc gccgcaaccc agacctcgtc gcggccgatc
     3361 gtcttgccca gctcggcgaa gctcaggcct ttttgttttt tggctgccag cagtctctcg
     3421 gtgatttcag gcgtgttcat ctgtctctcc tcttcacgag atcacgtaaa tgactttaca
     3481 acccacggct tacattctga gtcaactttc cagagtcaaa aacgaaaacc gattttcgcg
     3541 caaaggcgcg gagacgcagc gagagaaata atcgaccgtt tcggctttcc cctgcgcctc
     3601 tgcgcgagat cgctgtggct ttgaattcac gcttcatcat cgactccaca gatgcgatcg
     3661 gtcagtcccc tgtcatccgg gagcagcccc gcctccgcaa gcctcggtgt cagcgtcacc
     3721 cagtttttgt cccttgcgaa gaccttccgg aagagcggaa tggcttcttt gatccgcccc
     3781 atgttcacca gggcgattgc atgccagtac acgatctcca ggttgtccgg cgcgagccct
     3841 gctgcggcac cgtattcttc gagcgctttc gcgtggttct tgtgctccat ggccgcgtct
     3901 cccgcgttca tgtgattata tgcgcgctga agcacgagca ggcgccgcaa ctcccccagg
     3961 ggctcgggat ggtcatccac ccggagatcg aacgtccggt cggcccaggg acgtcccgtg
     4021 ggctttcccg cgacgatgag cgccgccgat tgcctgcccc gtacgtcccc gcccgcggcc
     4081 tgt
//
LOCUS       DASSLD010000287         5105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1063986, whole genome shotgun sequence.
ACCESSION   DASSLD010000287 DASSLD010000000
VERSION     DASSLD010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063986"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3419
                     /gene="mfd"
                     /locus_tag="VFK23_05195"
     CDS             <1..3419
                     /gene="mfd"
                     /locus_tag="VFK23_05195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET7318515.1"
                     /translation="MVLPVINDAGAGNKIVEVSELWGASKALFLFGLQKESNRPIIIV
                     TATEDSAGVLIEDLAFFVQKMPHDAQGEGAIEIRMFPSWGVLPFEADSPDSRTVGERM
                     RFLYCLISDSPGIYVVPAQALMQKLPPWELFADSVKTITPASRIEPDDLAASLIATGY
                     EPASLVTRVGEFSRRGGIIDFFSPLHDNPVRIEFFGDAIESLRAFDPESQRSTGELKE
                     AVVLPVRELIVKDPSDRPELSTSPGAEFLAPFFYNMETLFRYVPEDSLFVRIEPDDIT
                     NEMRVHGRKIEEGRAEETRDRRVLPEAREIYLDEEGITAGLSHFPVVDISLLGTGSGY
                     RMDTKSASWLGVRLTKPLEQDSQTSGSAEGTMAGLVEKLKGLREFNRVCIVSGTGEGA
                     ARMKKLFMEFGLGVALTGPAPASDPGRGPWPVMVTTGRLSEGFSWQDVGLVFITEEEI
                     FGRKIHPPRAGARPTKVAPFLSTFKELKPGDFVVHTDYGVGEYQGLTHISVDGFETDF
                     LTLRYEPDAKLHVPLYSLDKVQKYVGVEGAAPKLDRLGAPAWAKTKEKVKKDILAMAQ
                     ELVAIYAAREAQNRAPFTQPDNLYREFEAAFPYEETQDQQRAIEDVLADMQRPRPMDR
                     LVCGDVGYGKTEVALRAAFKAVEDGMQAAVLVPTKILADQHERTFSERLAPFSVRVEM
                     LSRFRTRSEQQETLKGLRSGTVDIVIGTHRLLQKDVVFKNLGLLVVDEEHKFGVKHKE
                     RIKEFKKLVDVLTLTATPIPRTLHMSLAGIRDLSIIQTPPLDRQAIQVVLARFGKRVI
                     REAIMNELVRGGRVFFVQNRVQGIERMADFIQNLVPEAKVGIGHGQLPEHEIEDVMTK
                     FIKGELNVLVTTTIIESGIDIPAANTIIINRADRFGLADLYQIKGRVGRSREKAYAYL
                     LTPADELLSDHARKRLRAIQELSELGAGFRIAAQDLETRGAGNLLGKQQSGHIAAVGI
                     DLYTQMMEEAVAGLRGEETSPEPDTQINMRASAFIPEDYIGDVSMRLAAYKEISAVSD
                     EAMLKDLADGLRDRYGELPEPAANLLEIMSVKIAAKRAGVARIDAGKESLTITFTGQA
                     AISPDKVMALLRKNRGRIRLIPEYTLQVSLPDETLRTASESVKKCLQELL"
     gene            3506..4198
                     /locus_tag="VFK23_05200"
     CDS             3506..4198
                     /locus_tag="VFK23_05200"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET7318516.1"
                     /translation="MDRLKLAVSISMLALILSATGCNKATSSSDGTVLAKVNRTTITT
                     AEFKKQLEELAPQMQQAVMSDPKARKEFLEDLIGIELVLQEAKRQGLDKDAEYKKRQE
                     TLKKELERRVQEDAKNELFNTVLKKEIGDKISKIAAPTDQEVKDYFNKNHDKIDTAAG
                     KKVSLKEVEPQLKMRLMQEKRRDLYLEYAKGLKAKAKITIDEKALDQAATALTQHKDV
                     DLSNMTAQPAPK"
     gene            4226..>5105
                     /locus_tag="VFK23_05205"
     CDS             4226..>5105
                     /locus_tag="VFK23_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET7318517.1"
                     /translation="MKNTRLTHLLAPALLLGLVFFSCKKPAAEKPAAPPVPASADVRV
                     VATVNGEPITLAEFQERYSRSGLKPDPEAALEVKREFLNRLIDRMMMLREAQRKRIKI
                     APQNITARIDAICAEEGKDVKDILSDLGLEFEKWKSDVWEDMMIERLVARDVNSHVSV
                     AQAEIRRYYEANRQEFDKPDQVRARQIVVETEQEAMKVREQLLAPGADFAAVARQTSK
                     APEAGKGGDLGFFAKGEMPAEFNVVFDLPKGGISGVVKSPYGYHIFKLEDMRKAGMIS
                     IADASREITDRLRHDKQ"
BASE COUNT         1187 a         1498 c         1518 g          902 t
ORIGIN      
        1 acatggtact gcccgtcatc aatgatgccg gggcaggaaa taaaatcgta gaagtgagcg
       61 aactctgggg ggcctccaag gccctctttt tattcggcct ccaaaaagaa tcgaaccggc
      121 ccatcatcat cgttacggcg accgaggatt ctgcaggtgt gctcattgaa gatctcgcgt
      181 tcttcgtcca aaaaatgcct catgacgccc agggggaagg cgcgatcgag attcgcatgt
      241 ttccctcctg gggggtgctg cccttcgagg ccgattcgcc cgacagccgc accgtgggcg
      301 agcgtatgcg tttcctctac tgtctgatct ctgattctcc cggcatttac gtggttccag
      361 cacaggcttt gatgcaaaag cttccgccct gggagctctt cgcggactcc gtaaagacca
      421 taacgccggc ctcccgaatc gagcctgacg accttgccgc ctctttgatt gccacaggct
      481 atgaaccggc gtccctggtc acccgcgtgg gagagttcag cagaagggga gggatcatcg
      541 atttcttctc tccgctccat gacaaccccg tccgcatcga attcttcggc gacgccatcg
      601 agtcccttcg cgcctttgat cccgaatccc agcgctcgac tggagagctc aaagaggcgg
      661 tcgtccttcc ggtgcgggaa ctcatcgtca aggatccgtc ggaccggcct gagctgtcca
      721 cctcgccggg cgccgagttt ctcgccccct tcttctacaa tatggaaacc ctgttccgct
      781 atgtgccgga agacagtctt ttcgttcgga tcgagcccga tgacatcacg aacgagatga
      841 gggtgcacgg caggaagatt gaagaaggca gggcggagga aacacgggac agacgcgtcc
      901 tgcccgaggc ccgggagatc tatcttgacg aggaggggat cacggccggg ctctcgcact
      961 ttccggtcgt ggatataagc ctgctcggta cgggctccgg ataccgcatg gacacaaagt
     1021 ccgcctcctg gttgggcgtc aggcttacca agcctctcga acaggacagc cagacaagcg
     1081 ggtccgccga gggcaccatg gcgggtctcg tggaaaaact gaaggggctc cgtgaattca
     1141 accgcgtctg catcgtctcc ggcaccgggg agggggcagc gcggatgaag aagctcttca
     1201 tggaattcgg actcggcgtg gcgctcaccg ggccagcccc ggcctccgac ccgggccggg
     1261 ggccctggcc ggtcatggtg acgaccggca ggctctcgga ggggttttcc tggcaggatg
     1321 tcggcctcgt cttcatcacc gaagaggaaa tattcggcag gaagatccac ccccccaggg
     1381 ccggggcaag gcccacaaag gtagcccctt tcctctctac cttcaaggag ctcaagcccg
     1441 gcgacttcgt ggtccatacg gactacggcg taggcgaata ccagggactc acacacatca
     1501 gtgttgacgg gttcgagacc gacttcctga ccctccgtta cgaaccagac gccaaactgc
     1561 atgtccctct ctacagcctc gacaaggtcc agaaatatgt cggcgtcgaa ggcgcggccc
     1621 cgaagctcga ccggctcggc gcgcccgcct gggcaaagac aaaggaaaag gtaaaaaagg
     1681 acatccttgc aatggcgcag gaactcgtgg cgatctacgc cgcgcgtgag gcccagaacc
     1741 gtgcgccctt tacccagccc gacaacctct accgtgagtt cgaggccgcc tttccctatg
     1801 aagagacgca ggaccagcag agggccatcg aggatgttct tgccgacatg cagcgaccgc
     1861 ggcccatgga ccggctcgta tgcggcgacg tggggtatgg aaaaacggag gttgcgctcc
     1921 gggccgcgtt caaggccgtt gaggacggga tgcaagcggc tgttctcgtg cccacgaaga
     1981 ttcttgcgga ccagcatgag cgcacctttt ccgagcgtct cgccccgttc tcggtccggg
     2041 ttgagatgct ttcgcgcttc cgcacccgct ccgagcagca ggagacgctt aaaggtctga
     2101 gaagcgggac cgtagatatc gtgatcggca cgcaccgcct gctccagaag gacgtagtct
     2161 tcaagaacct gggccttctt gtggtggatg aggagcacaa gttcggcgtc aagcacaagg
     2221 agaggataaa ggaatttaaa aaactcgtgg atgtgctgac tctcacggca acaccgatac
     2281 cccgcacgct ccacatgtca ttggcgggca tcagggacct ttcgatcatc cagacgccgc
     2341 ccctcgaccg gcaggcgatc caggtggttc ttgcgcgctt cggcaagcgg gtgatccgcg
     2401 aagccatcat gaacgagctt gtccgcggcg gtcgggtctt ctttgtgcaa aaccgcgtcc
     2461 agggcatcga gcgcatggct gatttcatcc agaaccttgt acccgaggca aaagtgggta
     2521 ttggccatgg gcagctcccc gagcatgaaa tcgaggacgt gatgacgaag ttcatcaagg
     2581 gagaactcaa cgtgctggtg accactacga tcatcgagtc ggggatcgac atccccgcgg
     2641 caaacacgat cattatcaac cgggccgacc gcttcggcct ggcagacctc tatcagatca
     2701 agggcagggt cgggaggagc agggaaaaag cttacgccta tctgcttaca cccgctgatg
     2761 aactgctttc cgaccatgca cgcaagcgac tccgggccat ccaggaactc tccgagctcg
     2821 gggctggatt ccgcattgct gcacaggacc tcgaaacgcg cggcgcggga aaccttctgg
     2881 gcaaacagca atcgggccac atcgcagccg tgggcatcga tctctatacg caaatgatgg
     2941 aagaggccgt cgccgggctt cgcggggagg agacgtcgcc ggagcccgac acgcagatca
     3001 acatgcgcgc ctccgcgttc attcccgagg actatattgg cgacgtaagc atgcggctgg
     3061 ccgcgtacaa ggagatctcg gctgtttcgg acgaggccat gctcaaagac ctcgcggacg
     3121 ggcttcgtga ccgctacggc gagctgcccg aacccgcagc gaatctcctc gaaatcatga
     3181 gcgtcaagat cgccgcgaaa agggccggcg tcgccagaat cgacgccggc aaagagagcc
     3241 tgacgatcac cttcaccgga caggcagcga tctcaccgga caaggtcatg gccttgctga
     3301 ggaagaacag gggaaggatc agactcattc cggaatatac gcttcaggtc tctctgcccg
     3361 acgagacgct ccggaccgcg tctgaatcgg tgaaaaagtg cttgcaggaa ctgctctagt
     3421 atgttataaa gtcctttgtt cgtctcacac cacaaaccac ttctagttct aaagacttaa
     3481 attcactcac ggaggaaacc attacatgga tagattgaaa ctggcggtaa gcatatcgat
     3541 gctcgccctc atcctgtccg ccacgggatg caacaaggca acatcatcct cggacggtac
     3601 ggtccttgcc aaggtgaacc ggaccacgat cacgaccgcg gagttcaaga agcagctcga
     3661 ggagctggct ccgcagatgc agcaggcagt catgtccgat cccaaggccc ggaaggaatt
     3721 tctcgaagac ctgatcggga tcgagctcgt gctgcaggaa gcgaagcggc agggactcga
     3781 caaggacgcc gagtacaaaa agaggcagga aacgctcaaa aaggagcttg agcgcagggt
     3841 ccaggaggac gccaagaacg aactcttcaa caccgtcctg aaaaaagaga tcggcgacaa
     3901 aataagcaaa atcgccgcac cgacggacca ggaggtcaag gattatttca acaagaacca
     3961 cgacaagatc gatacggcgg ccgggaaaaa ggtcagtctc aaggaagtcg agccgcagct
     4021 caagatgcgg ctgatgcagg aaaagagaag ggacctctac ctcgaatatg cgaagggcct
     4081 caaggccaag gccaagatta cgatcgacga aaaggcgctc gatcaggcgg cgaccgctct
     4141 gacccagcac aaggacgtcg acctgtcgaa catgacggcg cagccggcgc cgaagtaaga
     4201 atccgcaccg tcacaatagc gcaccatgaa aaacacccga cttacccatc tcctagcccc
     4261 ggcgcttttg ctggggctag tatttttttc atgcaagaaa ccggctgcag aaaagcccgc
     4321 agctcctcct gtccccgctt cggccgatgt cagggttgtg gcgaccgtca acggtgaacc
     4381 gattacgctc gccgaatttc aggaacgata ttcccggtcg ggactcaaac cagacccgga
     4441 agccgcactc gaggtgaagc gggagttcct caaccggctc atcgaccgca tgatgatgct
     4501 gcgcgaggcg cagcgaaaac ggatcaagat cgcgccgcag aatatcaccg ccaggatcga
     4561 tgcgatttgt gcggaagagg gaaaggatgt caaggacatt ctttccgacc tcgggctcga
     4621 attcgagaag tggaaatctg acgtctggga ggacatgatg atcgaacggc tggtcgcccg
     4681 ggatgtgaat agtcatgtct ccgttgcaca ggccgagatc aggcggtact acgaggcgaa
     4741 cagacaggag ttcgacaaac cggaccaggt ccgtgcgcgc cagatcgtcg tagaaaccga
     4801 gcaggaggcc atgaaggtga gggaacagct ccttgctccg ggagccgact tcgcagccgt
     4861 ggcgcggcag acgtcaaagg cgccggaagc gggaaaggga ggcgacctcg gtttttttgc
     4921 gaagggtgag atgccggcgg agttcaacgt cgtattcgac cttcccaagg gcgggataag
     4981 cggggtcgtc aagagcccct acggatacca catcttcaag ctcgaggaca tgcgcaaggc
     5041 cggaatgatc agcatcgccg acgcctcccg ggaaattaca gatcggctgc gccacgataa
     5101 gcagg
//
LOCUS       DASSLD010000288         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_236469,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000288 DASSLD010000000
VERSION     DASSLD010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236469"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..749
                     /gene="sodC"
                     /locus_tag="VFK23_05210"
     CDS             216..749
                     /gene="sodC"
                     /locus_tag="VFK23_05210"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HET7318518.1"
                     /translation="MKSLVIVVVFLLASLFAFAAVVSAEEALVQMNLITDQGIGKSIG
                     TVTLKDTPGGLVLTPALSGLAPGTHGFHVHEKPDCGPGTKDGKAVAGLAAGGHFDPGA
                     TGKHEGHMGKGHLGDLPALVAGADGAATTPVTAPRLKLSDVKGRSLMIHAGGDNFADS
                     PAPLGGGGARMACGVIK"
     gene            complement(837..1706)
                     /locus_tag="VFK23_05215"
     CDS             complement(837..1706)
                     /locus_tag="VFK23_05215"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015129432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7318519.1"
                     /translation="MKDKAEFTRGAVVITGTSTGIGRACALHLDRLGFRVFAGVRTSR
                     DAESLQRESARRITPLLTDVTEKASVRAAADAVTAELGDKGLSGVVNNAGIVVSGPLE
                     FLPESEIRKQFEVNVMGPIMVVQAFLPLLRKARGRIVNMSSISGRMALPFLGPYAASK
                     FALEAITDSLRVELLPWEIKVSLVEPGAVKTPIWEKSRVAAETTARNFPPGAYELYGR
                     ATAAFRSETEKADKAGVETEVVVRAVEHALTAKEPKTRYLVGRHVRLQASLKSALPDR
                     LFDWFISRRLEGS"
     gene            1881..2876
                     /locus_tag="VFK23_05220"
     CDS             1881..2876
                     /locus_tag="VFK23_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HET7318520.1"
                     /translation="MMKKNMFTIVAIIFTAFVSLAAGATDRVLIKEWDVPTPDSFPHD
                     PAVGPDGSLWYTGMFVNVLGRLDRTTNMIKEYRLKVPGSGPHGLAADKKGNIWFTANA
                     KGYIGRLDPQTGGVKEYYMPDPAARDPHSLAFDEKGMIWFTVQVGNFVGRLDPRSGAI
                     TLKPSPTRGSRPYGIAVNSKGVPFFCELGTNKIGSIDPVTMKISEYPLPEGARPRRLA
                     VAGDDMIYYTDYRRGYLGRLDAKTGKVVEWPSPGGRDSGPYGITITPDGAVWYSETEV
                     QPNAVVRFDPRTNGFGRWNVPSGGGVIRNMAATPGGDVYIACSGVNKIGIVTVEK"
BASE COUNT          630 a          818 c          865 g          604 t
ORIGIN      
        1 gacacctcgg aatgagctcc acgaaaaata tcagttttcc agacaggcca gatccttcac
       61 ttcgttcagg atgacaacct aaaacgtttt tcaaccggaa tgagacgaga tgttttgtca
      121 tttctgcgat caactgcatc ataccggtgt gctataatca cactgtcgga tcaaaacgtt
      181 gcgggtcacc atcataatac gaaggaggaa cggtcatgaa aagcttggtc atcgttgtcg
      241 tattcctgct cgcttcgctg tttgcctttg cagccgttgt ttctgcggag gaagccctgg
      301 tgcagatgaa cctcatcacg gaccagggga ttggcaagtc catcgggacc gtgacactga
      361 aggacacccc gggagggctt gtattgaccc cggcattgag cggactcgcg ccggggaccc
      421 acggattcca tgtacatgaa aagcccgact gcggtccggg gacgaaggac ggcaaggccg
      481 tggcaggatt ggcggcagga ggacatttcg atccgggtgc aaccggcaag catgagggcc
      541 atatgggcaa ggggcatctg ggcgacctgc ccgcgctggt ggctggcgcg gacggcgcgg
      601 caacaacacc ggtaacagcc ccgcgtctca agctctccga cgtgaaaggc cggtcgctca
      661 tgatccatgc gggcggcgac aacttcgcgg acagcccggc gccgctcggc ggtggcggcg
      721 caaggatggc gtgcggggtg attaaataag aaagagcgtg ctgttgaatt gcagcgtgtc
      781 gaactttaaa aggcggggga tgaccccgcc ttttctttgt ccgccgttca tgtcctttat
      841 gagccttcga gcctgcggct gatgaaccaa tcgaacaggc ggtcgggcag agcgctcttg
      901 agagatgcct gaaggcgcac atgacggccc acaaggtacc gggtcttcgg ttccttcgcc
      961 gtgagtgcat gttcgacagc gcgtacgaca acttctgtct cgacacccgc tttgtcagcc
     1021 ttctccgttt cggaacggaa tgcagcggtc gcgcggccgt agagttcgta ggcccccggt
     1081 gggaagttcc gggccgttgt ttccgcggct acacgggatt tttcccatat cggcgtcttc
     1141 acggccccgg gctcgacgag cgagaccttg atttcccagg gcaaaagttc aacgcggaga
     1201 gagtccgtaa tcgcctccag ggcaaatttg gacgccgcgt aaggtccgag gaagggcagt
     1261 gccattctgc cggaaatcga gctcatattc acgatgcggc ccctcgcctt gcgaagaaga
     1321 ggcaggaacg cctggacgac cattataggg cccatgacgt ttacctcgaa ctgctttcgt
     1381 atttcggatt cgggaagaaa ctccagcggg ccgctcacca cgatgcccgc attattcacc
     1441 acgccggaca ggcctttatc tccgagctct gcggtcaccg cgtccgcagc ggcgcgcacc
     1501 gaggcttttt cggtcacgtc cgtgagaaga ggagttattc tccgcgcaga ctcacgctga
     1561 agcgattcag cgtcccggct tgttctcacc cccgcaaaga cgcgaaatcc cagtctgtcg
     1621 agatggagag cgcaggccct cccgattccc gtggacgtgc ccgttatcac gaccgcgccc
     1681 cgtgtaaatt ccgctttatc cttcatgatg gtctccctca gcgtgaaaga tgtgtccgac
     1741 gacatcaact ataacataat cgcatcattg ttcgaggaaa acgggacttc gcgtgcatgc
     1801 ctcatgcact gaatgtatgc ggagtgtgct aggattattt caggaagaca tcgtaaatct
     1861 gttgcagtaa tccggaggcc atgatgaaaa agaatatgtt tacgattgtc gctattattt
     1921 ttactgcatt cgtatcactc gcggcaggcg cgacggaccg ggtattgata aaagaatggg
     1981 atgtgccgac cccggactca ttcccccacg accccgccgt ggggccggac ggatcgcttt
     2041 ggtacaccgg catgtttgtc aacgttttgg gaaggctgga ccggacaact aatatgatca
     2101 aggaatatcg tttgaaggtc cctggttcag gtcctcacgg actcgcggcg gacaaaaagg
     2161 gaaacatttg gtttaccgcc aacgccaagg gctacatcgg caggctcgat ccgcagacag
     2221 gcggggtgaa ggaatattac atgcctgacc ctgcggcccg tgacccgcat tcgctggcct
     2281 tcgacgaaaa aggcatgatc tggtttaccg tgcaggtcgg caactttgtc ggaagactcg
     2341 atccccgctc cggagctatc accttgaagc cctcgccgac gcgcggttcg agaccctatg
     2401 gcatcgctgt gaactcgaaa ggggtccctt ttttctgcga actcgggaca aataaaattg
     2461 gaagcatcga ccccgttacc atgaagatat ccgaatatcc tctgccggag ggggccaggc
     2521 cgcggcggct ggccgttgcc ggcgacgaca tgatttacta cacggattac cgccgcgggt
     2581 atctcggccg gctcgacgcg aagaccggca aagtcgttga gtggccctcc cccgggggcc
     2641 gcgattcggg gccctacggc atcacgatta ctccggacgg cgcggtgtgg tacagcgaaa
     2701 cagaggtaca gccgaacgcg gtcgtacgat tcgatccgcg aacaaacggt tttggcaggt
     2761 ggaacgtccc ctcgggcgga ggggtgatcc gcaatatggc tgcaactccg ggtggggatg
     2821 tctacatcgc ctgcagcggc gtcaacaaga tcgggatcgt gaccgtagag aaatgatctg
     2881 ttccgggcag cccgcattcc ggagaaagga atatcta
//
LOCUS       DASSLD010000289         2456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1602186, whole genome shotgun sequence.
ACCESSION   DASSLD010000289 DASSLD010000000
VERSION     DASSLD010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602186"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VFK23_05225"
     CDS             complement(<1..1034)
                     /locus_tag="VFK23_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HET7318521.1"
                     /translation="MSPRSKREYLAAIVKRYRSASRSIKTVILNEFCSTCGYHRKHAL
                     RLIRTFRRFTAKTPAKRGRRPLYDPELLRTPLSRIWKAANLPCSKRLKAIIALWLPGY
                     IKTYGPIPEKTAIALLRISAPTIDRVLRPVRAEYAGHGRSTTKPGTLLKRHIPVKVNQ
                     WDESRPGFLEADTVAHCGSTTDGMFVNTIDCVDIATGWTEQRAVWGKNHQDVITQIKD
                     IERCLPFPILGFDVDNGSEFLNHHLLRYLTDNRVRPVQFTRSRAYHKDDNAHVEQKNW
                     THVRQWIGYDRLDRPGFVSLMNELYTSEWRLFLNFFCPSVKLIEKKRIASKTVKRYDK
                     PKTPYQRVMES"
     gene            1192..>2456
                     /locus_tag="VFK23_05230"
     CDS             1192..>2456
                     /locus_tag="VFK23_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04442 family protein"
                     /protein_id="HET7318522.1"
                     /translation="MIHEIKLHGQANEKLDYFVTITGADLSSRYCYESLPEGDRFFSG
                     RNEFVITPTGISYTGTGGGLCEYMFGVDLPLKDLLRKDVSNRLVMYGAFYDKSDNITF
                     TNNTSGKESFDHVFLTGNAVSNYYFFVHTSRRGEIRDLQRDILRSIGKQLKRSTTVGA
                     ADDTRLCREIFEALDDPKALVFLFRLLNRRNEEYFNAFNRLYAAHKSMTTKSLMELEE
                     MAHNYGIVPYQQERIKIDGMYKLPENKKIVDEYKDILISVTEKGEVNPSELAKLSRLR
                     TLALRLNIPHNLFDTLDELLLKDMNIVEIEEPDYVRETRAIFEGLFFQTDKIKGHIST
                     EDLVKLLLAKQKSTLVRDQAFEGLLLDTVRACDEHARDRNDTSVLEVMSSILTYFDRF
                     DSTATAINNLAFMENSVLTEDNIRSIFGN"
BASE COUNT          567 a          699 c          654 g          536 t
ORIGIN      
        1 gactccatta cccgctggta tggtgttttg ggcttatcat agcgtttcac ggtttttgat
       61 gcaatacgct tcttctcgat cagctttacc gaagggcaga agaagttcag aaacagccgc
      121 cactcagagg tatagagctc gttcatgagg gaaacgaacc ccgggcgatc cagacgatca
      181 tagccgatcc attgccgcac atgagtccag ttcttctgct ccacatgggc attatcgtcc
      241 ttgtgatagg ccctgctcct ggtaaactga accggacgga ccctgttgtc ggtcagatag
      301 cggagcaggt ggtggttcaa gaactcactg ccgttatcaa cgtcaaaacc cagaatcggg
      361 aacggcaggc agcgctcgat atccttgatc tgggtgatga cgtcctggtg gtttttgccc
      421 cagacggcac gctgttccgt ccatccggtt gcgatgtcca cgcagtcgat agtattgaca
      481 aacatgccgt ctgtggtgct tccgcagtgg gcgacggtgt cggcttccag gaagcctggc
      541 cttgattcat cccattggtt cactttgacc gggatatggc gtttcaggag cgttcccggc
      601 ttggttgtac ttctgccgtg gcctgcatat tccgcacgga caggccttaa gacccggtca
      661 atggtcggcg cagagatccg gagcagtgct atggcggttt tctccgggat cgggccataa
      721 gttttgatat accccggcag ccacagggca atgatggcct tcaggcgctt tgagcacggc
      781 agattagccg ccttccagat gcgggaaagc ggtgtcctaa gcaattccgg gtcatagagc
      841 ggccgtcttc cccgtttggc cggggttttt gccgtaaacc tccggaaggt ccgaatgagc
      901 ctcagcgcgt gcttacggtg ataaccgcag gtagagcaga actcattcaa gatgaccgtt
      961 ttaatactcc gggaagctga ccgatatcgt tttacaatgg cggccagata ctcccgttta
     1021 gatcgtggac tcatgggaac gtaccctcct aatcgaggta tagttccggt aacatgcatt
     1081 atgagtcaac gatagttttc aaagcacgct tgggtaacat ttaatatgag tcaattcgcg
     1141 catggatttt tatttaaaaa atcaagccgg tttgctataa tacggactgg tatgatccac
     1201 gaaatcaaat tgcatggcca ggccaacgaa aagctggact acttcgtcac catcacgggc
     1261 gcggatctga gcagcaggta ctgctacgaa tcgcttcccg agggcgatcg gttcttctcc
     1321 ggcaggaatg aatttgtcat cacgccgacc ggcatttcct acacggggac cggcggcggg
     1381 ctctgcgaat acatgttcgg cgtggacctg ccgctcaagg acctcttgag gaaggacgtc
     1441 tcgaaccgtc tggtcatgta cggcgccttc tacgacaagt ccgacaatat aactttcacg
     1501 aacaacacct ccggcaagga gtcctttgac cacgtcttcc tcaccggcaa cgcggtctca
     1561 aactactatt tcttcgtcca cacgtcgcgc cggggagaaa tcagggacct ccagcgggat
     1621 atcctgagga gcatcggaaa gcagctcaag cgaagcacga cggtgggcgc cgcggatgac
     1681 acccgcctct gccgcgagat tttcgaagcc ctggacgatc ccaaagccct cgtgttcctc
     1741 ttccgcctgc tgaaccgccg aaacgaggaa tatttcaacg ccttcaatcg gctctatgcg
     1801 gcgcacaagt ccatgaccac caagtcgctg atggaattgg aagagatggc gcataattac
     1861 gggatcgtcc cctatcagca ggaacgcatc aagatcgacg ggatgtacaa acttcccgag
     1921 aacaagaaga tcgtcgacga atataaggac atcctcatct cggtgacgga aaagggcgag
     1981 gtgaacccgt ccgagttggc caagctctcg cggctccgca cgctcgccct gcgcctcaac
     2041 attccgcaca acctcttcga cacgcttgat gaacttctcc tgaaggacat gaacatcgtc
     2101 gaaatcgagg agcccgacta cgtccgggag actcgcgcga tcttcgaagg gctgttcttc
     2161 cagaccgaca agatcaaggg gcacatctcc acagaagacc tcgtcaaact cctcctggcc
     2221 aagcagaagt caaccctggt ccgcgaccag gccttcgagg gcctgctcct ggacacggtc
     2281 cgcgcctgcg acgagcacgc ccgcgatcgg aacgacacga gcgtgctcga ggtcatgagc
     2341 tccatcctca cctatttcga ccgtttcgac agcacggcga cggccatcaa caacctcgcc
     2401 ttcatggaaa attcggtgct taccgaggac aacatcagga gcatattcgg caacaa
//
LOCUS       DASSLD010000290         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_395471,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000290 DASSLD010000000
VERSION     DASSLD010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395471"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFK23_05235"
     CDS             complement(<1..264)
                     /locus_tag="VFK23_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HET7318523.1"
                     /translation="MTRHIPSTRRAILILLAGLLFACKSDTLSYRTEPVTRGDIQATV
                     TATGTVNAVTTVQVGTQVSGTIKTLYVDFNSRVKKRQLIAQIDP"
     gene            complement(274..>2068)
                     /locus_tag="VFK23_05240"
     CDS             complement(274..>2068)
                     /locus_tag="VFK23_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7318524.1"
                     /translation="SLVAVFIPVLFMGGILGRLLHEFAVVISVSIIVSGVVSLTLTPM
                     LASRFLRPHGDERHGRVYMALERFFDGMRNGYKTSLAWVLQHRSLTLAATVALFIATI
                     FLFIIMPKGFLPTEDTGQIFAFTEAQQGISFPDMARHQQQLVDIIAQDPNVEAFMSSI
                     GAGGPNAAGNTGRIFMRLKPRSERTMSADEIIQSLRKKTAGVIGIRIFLQNLPPIRIG
                     GTLTKSQYQYTLQSPDTDELYRVAPLLEAKLRTIPQLQDVNSDLQIKNPQVNLEIDRD
                     KASALGVTAEQIEIALSTAYSSPQISTILAPTNQYQVIMELLPEYQMDPTSLSMLYVR
                     SSSGKLVPLNVVTKPTVGTGPLLVNHVGQLPAVTLSFNLRPGVSLSEAVALVDKASRE
                     TLPASVTTSFQGTAQAFQSSTQNLFLLLIMAVLVIYIVLGILYESFIHPLTILSGLPA
                     AGVGALLTLMLFGKELNIYGYVGVIMLIGIVKKNAIMMIDFALEAERKEGKGPAEAIF
                     EGALVRFRPIMMTTMAALMGTLPIALGLGAGAEARRPLGLAVVGGLVFSQLLTLYITP
                     VIYTYLDTFQRRFGRLAIFRTREEIPNAGHE"
BASE COUNT          409 a          585 c          658 g          416 t
ORIGIN      
        1 tgggtcgatc tgtgctataa gctgcctttt tttcacgcgg gaattgaaat caacatacag
       61 ggtcttgatc gtgcccgata cctgtgtgcc gacctggacc gtcgtaaccg cgttgacggt
      121 ccctgttgcc gtcacggtcg cctggatgtc gccgcgcgtt accggttcgg tccggtagga
      181 cagtgtgtcg cttttgcagg caaagaggag gcccgcgagg agaatgagaa tcgctctcct
      241 ggttgacggt atgtgcctgg tcatgtgcgt tccttactca tgtcccgcat tcggtatctc
      301 ctccctggtc ctgaagatcg cgagccggcc gaaacgcctc tggaacgtgt ccaggtacgt
      361 gtaaatgacc ggcgtgatgt acagggtcaa aagctgcgaa aagacaaggc cgccgaccac
      421 ggcgagcccg aggggcctgc gcgcctccgc tcccgcgccc aggcccagcg cgatgggcag
      481 cgttcccatg agggctgcca ttgtggtcat catgatggga cggaacctga cgagggcgcc
      541 ctcaaagatc gcctccgccg ggcctttccc ttccttccgc tccgcctcga gcgcgaaatc
      601 gatcatcatg atggcgttct tcttcacgat gccgatgagc atgatcacgc cgacgtaccc
      661 gtagatgttc agttccttgc cgaagagcat cagggttaaa agcgccccca cgccagcggc
      721 gggcagtccg gaaaggatcg tgagcggatg gataaagctc tcatagagga tccccagcac
      781 gatatagatg accaggaccg ccatgattaa aaggagaaac aggttctgcg tggatgattg
      841 gaacgcctgc gcggtcccct ggaagctcgt cgtgaccgaa gccgggagcg tttcccgcga
      901 cgccttgtcc acgagagcaa ccgcctcgct cagggacacg cccggcctca ggttgaagga
      961 gagggtcacg gccgggagtt gtccgacatg attgacgagg agcggtcccg tacccaccgt
     1021 cggcttggta accacattga gcggcaccag tttgccggag gatgaacgca cgtagagcat
     1081 ggacagcgag gtcggatcca tctgatactc cggcaagagt tccatgatga cctggtactg
     1141 gttggtcgga gcgaggatcg tagatatctg cggcgagctg tacgccgtgc tcaaggcgat
     1201 ttcgatctgt tccgcggtaa cgccgagtgc ggaagccttg tcgcggtcga tctcgaggtt
     1261 cacctgcgga ttttttatct gcaaatcgct gttcacgtcc tggagctgcg ggatcgtccg
     1321 aagctttgcc tcgaggaggg gcgcgacgcg gtagagctcg tccgtgtccg ggctctggag
     1381 cgtatactgg tactggctct tggtgagcgt gccgccaatg cggatcggcg gcaggttctg
     1441 caggaaaatc ctgatgccga tgacgcctgc cgtctttttc cggagcgact ggatgatctc
     1501 gtcggcggac attgtcctct cggaacgggg tttgagcctc atgaagatcc ggccggtgtt
     1561 tccggcggca ttcggacccc cggcgccgat cgacgacatg aacgcctcga cattggggtc
     1621 ctgggcgatg atgtcgacga gctgctgctg atgcctcgcc atatcgggaa aggagatccc
     1681 ctgctgcgcc tcggtaaatg cgaagatctg tcccgtgtct tccgtcggga ggaatccctt
     1741 gggcataatg atgaacagga atatcgttgc aatgaagagc gccaccgtag cggcaagggt
     1801 caggctgcga tgctggagca cccaggcgag gcttgtctta tatccgttcc gcatgccgtc
     1861 gaaaaaccgc tcaagggcca tgtatacgcg cccatggcgc tcgtctccgt gaggacggag
     1921 aaaccggctc gccagcatcg gcgtgagtgt cagggacacg acgccggaaa cgataatgga
     1981 tacgctgatc acgacggcga actcatggag gaggcggccg aggatgcctc ccatgaagag
     2041 cacggggata aagaccgcga ccagggag
//
LOCUS       DASSLD010000291         3526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1043205, whole genome shotgun sequence.
ACCESSION   DASSLD010000291 DASSLD010000000
VERSION     DASSLD010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043205"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..669)
                     /locus_tag="VFK23_05245"
     CDS             complement(235..669)
                     /locus_tag="VFK23_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7318525.1"
                     /translation="MRNGSSWRRGRYPYTADETTDRQVLCYTNAYISKKGEHEMRKIL
                     LGFFVLVFCCSAGYVFAEKGSDKGEAKFKALCAVCHPDGGNVMNPKKTLHKQDREANN
                     VKTADDIVAKMRNPGPGMTKFDEKTVSNKDAHAIAEYVLKTF"
     gene            complement(638..889)
                     /locus_tag="VFK23_05250"
     CDS             complement(638..889)
                     /locus_tag="VFK23_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318526.1"
                     /translation="MVIANEEIKELIAEVPEGHRHLRTTLVLQDGREITLQEATVANL
                     VRAYITVKTHPARDSLRLHGRRLASRKEGFAEWQLLETG"
     gene            complement(1116..1976)
                     /locus_tag="VFK23_05255"
     CDS             complement(1116..1976)
                     /locus_tag="VFK23_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET7318527.1"
                     /translation="MNNYLKIIAAMLIWSTWGPMIRWLALPPVVVLFYTSLVASFTVP
                     TFLAARGELNLAGVGKSWWLFILLALSSITNNITYFYALGHTTVSNAVFTHYTAPLFV
                     ALLAPLMISERLRKVTLLSLPIALAGMSLIVMSGGGLRVDSAHTPGIIAGTVSGVAYA
                     FIIIFSRKLSQMLMHHKAVVVLLWITTITLSPVVFLTEHHIDLRGGVLLFLTGILHST
                     TAPLLYFNALRKVIAQHAAILGYMEPLAAIPMAFLLLSETPAVTSLAGGALILLSGYL
                     VIRARADSES"
     gene            complement(2113..3522)
                     /gene="ahcY"
                     /locus_tag="VFK23_05260"
     CDS             complement(2113..3522)
                     /gene="ahcY"
                     /locus_tag="VFK23_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET7318528.1"
                     /translation="MESSMSTKDYVVADINLASWGRKEIAIAETEMPGLMAIRAEFAK
                     TQPLKGARITGSLHMTIQTAVLIETLKALGAEVRWASCNIFSTQDHAAAAIAAGGTPV
                     FACKGESLRDYWDYTHRIFEWPDGGYSNMILDDGGDATLLLHLGSRAEKDSSVLDKPD
                     SEEATVLFASVKAKLKQDPKWYSTRLAKIKGVTEETTTGVHRLYQMHEKGELKFPAIN
                     VNDSVTKSKFDNLYGCRESLVDGIKRATDVMVAGKIAVVCGYGDVGKGSAQALRALSA
                     QVWVTEIDPICALQAAMEGYRVVTMDYACDKADIFVTATGNYHVIDHQHLVKMKNQAI
                     VCNIGHFDSEINVASLEKYQWEEIKPQVDHIIFPDKKRIILLAKGRLVNLGCATGHPS
                     YVMSSSFANQTIAQIELFTHTDKYPVGVYTLPKQLDEKVARLQLKKLNAQLTTLTDQQ
                     AAYIGVPKEGPYKSGHYRY"
BASE COUNT          725 a          928 c         1028 g          845 t
ORIGIN      
        1 tcatataagg acctcctata aggaaattta attacaatac aagtgtaaag tgtagattaa
       61 agtaacaaag ttgtcaagat caaccctaaa atattagtcg ttaaacggtt gttaattttt
      121 gcaacttctg cagtcggata aacacggatt gagaacgctc cgtcattttt ttgggcgctc
      181 ttcagatcac ctttttacga atcagtatca ggaagtcctt tgcaagttct tgtattagaa
      241 cgttttcaga acatattctg caatggcatg ggcatccttg ttagacactg tcttttcgtc
      301 gaacttggtc atgcctgggc cggggtttct catctttgca acaatatcgt ccgcggtctt
      361 gacattgttt gcttcccggt cttgcttgtg cagggttttt ttcgggttca tgacattccc
      421 cccgtcggga tgacacacgg cgcacagtgc cttgaatttt gcctccccct tgtccgatcc
      481 cttttcagcg aatacatacc cggcagagca gcagaaaacc agcacgaaga agcccaacaa
      541 aatttttctc atttcatgtt ctcctttttt tgaaatatag gcattagtat agcataacac
      601 ctgcctgtcc gttgtctcat cggcggtgta tggataccta ccccgtctcc aggagctgcc
      661 attccgcaaa tccttctttc cgtgaagcca gtctccggcc gtgaagacgc aggctgtcac
      721 gggcgggatg ggtctttacg gtaatgtacg cgcgtacaag atttgccact gtcgcttcct
      781 gcagggttat ttcccttccg tcctggagga caagcgtcgt cctcaggtga cggtgacctt
      841 cagggacctc tgcgatcagt tccttgatct cttcgtttgc aatgaccatg gaatgcctcc
      901 gtcctattca gtggttttac taatttatgc aagaatcgta agagtcttct tagccgcaaa
      961 atgcccataa tgcggagaga aagtcgtgag ttttttcgaa ttatctattt gctttttgtg
     1021 atttatgtgg ccaatcttga ctttacggct tcatcaataa ttatagatga tcaaacgctt
     1081 ccggcggccg gggcggaaga atcccctgta tcggttcagg attcagaatc ggcgcgggcg
     1141 cgtatcacga gatagcctga cagaaggatc aacgcgccgc cggcgaggga ggtgaccgcg
     1201 ggggtctccg acaggagcag gaacgccatg gggatggcgg cgagcggttc catgtagccg
     1261 aggatcgctg cgtgctgcgc gataaccttc cggagggcgt tgaaataaag cagcggtgcg
     1321 gtggtggaat gcaggatgcc ggttaggaaa agaaggaccc ctcccctgag gtcaatatga
     1381 tgttccgtga ggaagaccac cggggacagc gtgatggtcg tgatccacag gaggacaaca
     1441 acagccttgt gatgcatgag catctggctt aattttctcg agaagatgat aatgaaggcg
     1501 taggcaacgc cggataccgt gcccgcgatg atccccggcg tgtgagcgct gtcgacccgg
     1561 agtccgccgc ccgacatgac gattagagac atgcccgcga gggcgatcgg cagcgacagg
     1621 agggtcactt tccggagacg ctcggaaatc atgagcgggg cgagaagggc cacgaacagc
     1681 ggcgcggtgt agtgcgtgaa cacggcattc gacaccgtgg tgtggccgag cgcgtaaaaa
     1741 taggtgatgt tattcgttat cgatgaaagc gcgagaagga tgaacagcca ccatgatttc
     1801 ccgacgccgg cgaggttcag ctcgccccgc gcggccagga acgtgggcac cgtgaagctt
     1861 gcgacaagag acgtataaaa aaggaccaca acgggaggca aagccagcca gcggatcatg
     1921 ggaccccatg tgctccagat cagcatggct gcgatgattt ttaggtagtt gttcatgtca
     1981 attcggaatg cggatggccg atgaaacggg ccatgctgat tcggaatgcg gaataaagtc
     2041 gaaaaaaatg cggaatgcaa ggcgtattaa acgttccttc acattccgca ttccgaattc
     2101 ggaaaagccc gtttagtagc ggtagtgccc cgacttgtac ggtccttcct tgggcacccc
     2161 gatgtaggcg gcctgctgat cggtcagtgt ggtcagctgc gcgttcagtt ttttcagttg
     2221 caggcgcgcg accttttcgt ccagctgctt cggcagcgtg tacacgccca ccgggtactt
     2281 gtcggtatgc gtgaacagtt cgatctgggc gatggtctga ttggcgaagg aggacgacat
     2341 cacgtacgac gggtggccgg tggcgcagcc gaggttcacc agacggccct tggccagcag
     2401 gatgatgcgc ttcttgtccg ggaagatgat gtggtcgacc tgcggcttga tctcttccca
     2461 ctggtatttc tccaatgagg cgacgttgat ctcggagtcg aagtggccga tgttgcagac
     2521 gattgcttgg ttcttcatct tcaccaggtg ctggtggtcg atgacgtgat agttccccgt
     2581 ggccgtcacg aagatatcgg ccttgtcgca ggcgtagtcc atggtcacca cgcggtagcc
     2641 ttccatcgcg gcctgcagcg cgcagatcgg gtcgatttcg gtgacccata cctgggcgga
     2701 caatgcgcgc aacgcctggg ctgagccctt gcccacgtcg ccgtagccgc agaccacggc
     2761 gatctttccc gcaaccatta catcggtggc gcgcttgatg ccgtccacca gcgattcgcg
     2821 gcagccgtag aggttgtcga atttcgattt tgtgaccgag tcgttgacgt tgatcgcggg
     2881 gaacttgagt tcgcccttct cgtgcatctg gtagaggcga tgcacgcccg tggtggtctc
     2941 ctcggtcacg cccttgatct tggccaggcg cgtcgagtac cacttcgggt cctgcttgag
     3001 cttggccttg acgctggcaa acagcaccgt cgcttcctcg ctgtcgggct tgtcgagcac
     3061 cgagctgtcc ttctcggccc tgctgccgag gtgcagcagc agtgtggcgt cgccgccgtc
     3121 gtcgaggatc atgttcgagt agccgccgtc cggccattcg aagatacggt gcgtgtagtc
     3181 ccagtagtcc ctgagcgatt cgcccttaca ggcgaacacc ggcgtgcctc ccgcagcgat
     3241 ggcggcggca gcgtggtcct gggtcgagaa gatgttgcac gaagcccagc gcacttccgc
     3301 gccgagcgcc ttcagcgtct cgatcagcac ggcggtctgg atggtcatgt gcagcgaccc
     3361 ggtaatgcgc gcgcctttca gcggctgcgt cttggcgaat tctgcgcgga ttgccatcag
     3421 gccgggcatt tcggtttcgg cgatggcgat ttctttgcgg ccccacgagg ccaggttgat
     3481 gtcggcgacg acgtagtctt tggttgacat agagctctcc attcaa
//
LOCUS       DASSLD010000292         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_754323,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000292 DASSLD010000000
VERSION     DASSLD010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754323"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /locus_tag="VFK23_05265"
     CDS             <1..154
                     /locus_tag="VFK23_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET7318529.1"
                     /translation="LSARDAEYLHERFPMGSCLAVLNKFEVQQQGVLDMIFKSLQEKS
                     AGKGKG"
     gene            complement(190..600)
                     /locus_tag="VFK23_05270"
     CDS             complement(190..600)
                     /locus_tag="VFK23_05270"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET7318530.1"
                     /translation="MTDAQHTGPPEGPAGEFIEKRLEERFSVPPVYERYITLRVKSGD
                     EFATCVLGNFSRSGILFVSQAPFEADSHAECVIATPSLLSREVVFGIRVKYCLPKEGS
                     FFVGAAIETVADRLLFDIFAEVHDYIVQRQDDVY"
     gene            765..1709
                     /locus_tag="VFK23_05275"
     CDS             765..1709
                     /locus_tag="VFK23_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET7318531.1"
                     /translation="MNITAYRRVTQIAFVLLIFLMPVFDIFRYDTAAHELIVFGNSWG
                     LGLKDGFAADQSATAALQVAAQFFLKAILPWLIFLALFPLLGYFTGRFFCGWLCPEGA
                     LFELADFLTLRIIGRRSLYAKKPNDPEVPRRGRIKYAGVALLSAVIIPLLGGVALTGY
                     FVAPKTIWHQLMTWNFTFGVKAGIIGVAIYMLITSVLVRHILCKFVCAAGLMQMLFGW
                     ISPVSLRLRMDAGRISECTDCRGCDRACFMNVVPRKNKRDISCVNCGACVVACRKELG
                     AERGLFELGFGTKKAAAASRVPSPAGEAAYAESVEQPR"
     gene            1902..>2259
                     /locus_tag="VFK23_05280"
     CDS             1902..>2259
                     /locus_tag="VFK23_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HET7318532.1"
                     /translation="MAIQWTEDLATNVPEIDAQHKELFSRINRLLEACNQGQGRSEVG
                     KTLAFLEEYVLIHFATEEKIMTGRLYPECAGHKAQHLQFMSNLDALKQQFAKEGPTVH
                     LTLLTNHTVVDWLRNHI"
BASE COUNT          500 a          653 c          635 g          471 t
ORIGIN      
        1 cctgtcggcg cgcgacgcgg agtacctgca cgagcgtttc cccatgggaa gctgcctcgc
       61 cgtgctcaac aaattcgagg tccagcaaca gggcgtattg gacatgatct tcaagtcttt
      121 gcaggaaaaa agtgcgggaa aaggaaaggg ctgaagagaa agctcaaggc aacgcccctg
      181 agacatcggt taataaacgt cgtcctggcg ctgcacaatg taatcgtgaa cttcggcaaa
      241 aatgtcaaac aagagccggt cggcaacggt ttcgatcgcc gctccgacga aaaaggaacc
      301 ctctttgggg agacaatatt tgacgcgaat gccgaagacg acttcccggg acaagaggct
      361 tggcgtcgcg atgacgcact ccgcatgcga atcggcctca aagggcgcct ggctcacgaa
      421 caaaatgccc gatctgctga agttgcccag gacacaggtt gcgaattcat cgccggattt
      481 cacccgcaag gttatatagc gttcgtatac ggggggaacg ctgaagcgtt cttccagccg
      541 cttttcgata aattcccctg ccggtccttc aggcggaccc gtatgctgtg catcggtcat
      601 ggtcatttcc ttatatagct taaggagaga atgactttag aaaaaacttt tccatttttc
      661 ttagcagata gacggcaaaa tagcaagtgg aaaagggtga agtaattccc cgggctatga
      721 cctgcatcat aacccttttc ccccgcctgc ggtatatatt gagcatgaac attaccgctt
      781 acagacgagt gacccagata gcgttcgttc tcctgatctt ccttatgccg gtgttcgaca
      841 tattccggta cgatacggcg gcccacgagt tgatcgtctt cggaaattcc tggggcctgg
      901 gtctgaagga cggcttcgcc gcggatcaga gcgccacggc cgccctccag gtcgccgcac
      961 aattcttcct gaaggccatt ctcccctggc tcatatttct cgcactgttc ccgctcttgg
     1021 ggtatttcac cggccgcttc ttctgcggct ggctctgccc cgagggcgca ctcttcgaac
     1081 tcgcggactt cctgacgctg cggatcatag gcaggagaag cctgtacgcg aaaaaaccga
     1141 acgaccccga ggtcccgcgc cggggaagga tcaaatatgc cggcgtcgcg cttttgagcg
     1201 cggtcataat ccccctgctt ggcggagtgg cgctgaccgg ctacttcgta gccccgaaaa
     1261 cgatatggca tcagttgatg acgtggaact tcaccttcgg cgtcaaggcc gggatcatcg
     1321 gcgtcgcgat ctacatgctc atcacctcag tcctggtgcg tcacatcctc tgcaagttcg
     1381 tctgcgccgc gggcctcatg cagatgctgt tcggatggat cagtcccgtg tctttgaggc
     1441 ttcgcatgga tgcggggcgg atctcggagt gcaccgattg cagaggctgc gacagggcct
     1501 gtttcatgaa cgtggtcccg agaaagaaca agcgggacat ctcgtgcgtg aactgcggcg
     1561 cctgtgtcgt ggcctgccgg aaggaactcg gcgccgaaag gggcctgttt gaactcggct
     1621 tcgggacaaa gaaagctgcg gccgcaagcc gcgtgccgtc gccggcaggt gaagcagcgt
     1681 acgctgaaag cgtggaacag ccgcgctgaa cctccgccgc ttcctgaatc gtatagacgc
     1741 cgatgaccgg cctccggtct ctctccccct cttccgaaac attcatggag tttccgatca
     1801 ccgcttgaca atggtttctt ccgtggtaca ttaagaaaag tgctcacacg cgtcccgccg
     1861 gtccgggcga cgcatccgaa ggcaaccgca gaggaggctt catggcgatt caatggacgg
     1921 aagatctggc gaccaatgta ccggaaatcg acgcgcagca taaagagctg ttcagccgca
     1981 tcaaccggct gctggaagcc tgcaatcagg gccagggccg gtccgaggtc ggaaagaccc
     2041 tcgcgtttct cgaagaatac gttctgatcc atttcgccac ggaggagaag atcatgaccg
     2101 ggcgtctcta ccccgagtgc gcgggacaca aggcgcagca ccttcaattc atgtcgaacc
     2161 tcgacgccct gaagcagcag ttcgcgaagg aaggccctac cgtgcatctc actttgctca
     2221 ccaatcatac ggtagtcgac tggctccgga accatatca
//
LOCUS       DASSLD010000293         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_331913,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000293 DASSLD010000000
VERSION     DASSLD010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331913"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..1637)
                     /locus_tag="VFK23_05285"
     CDS             complement(240..1637)
                     /locus_tag="VFK23_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7318533.1"
                     /translation="MRGIDRSKKWGLAVFAALVFGTVPLHPLFGEEAAQPGSPLPAAG
                     QSEKNNDRSGGGKTASVETLTLSRAIEIALEKNPTILAARFGVDVSSNRVGEARSAYY
                     PQLSASAAYTRINPVPGSSTFGGNETFNQFSSSVTLNQNILDFGKTSSQVDVSKYNLE
                     ASRDDLQSTQDSVIQSVKQDYYGVLQARRNRDVAEDVIKQFQLHLDQAKGFYDVGTKA
                     RIDVIKAEVDLSNAKLDLINKDNAFKIAWVTLKNVMGVPDAPEGTYTIEEKLTFEKYT
                     ITLDEAVSHALDKRPDYKSAVAKRQAAESNISLQQSGHYPVLSGNASYNWAEIDQSPY
                     QLNHGWSAGVVLTVPLFSGFLTSHQVAEAKANLYILKANEESTRQQIMFDVRQAYLNL
                     QAAEASIATAQLAADQAKENMDLANGRYAAGVGSPIEVSDAFATYVTAQANYTSALSN
                     YKIAQASIERAMGAR"
     gene            complement(1624..>2139)
                     /locus_tag="VFK23_05290"
     CDS             complement(1624..>2139)
                     /locus_tag="VFK23_05290"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7318534.1"
                     /translation="TLFRHFGSKELLFEEVLKRYTFLPKLKELLPELEGMSCEEALVL
                     IATRFLTTLKERKSLIKIMYSEVTSYPDKIKEVYDRFIHEMRATLAAYFEDLKQRGIL
                     RSNLSAELAARLFLWILFSYFRSEEIMRPSGMKKHVMEKEIREMIDIYTHGTMNCDHK
                     KGHGGERYAGN"
BASE COUNT          425 a          617 c          548 g          549 t
ORIGIN      
        1 cgggacgttc tgctgagtca cgtcgcctgt ttttaccggc accacccgct ggggcgtctg
       61 tttagccttg ccgtcggaac agcctccggt aaaagcgaaa aggagaaaca gggcgatgaa
      121 tgccgtgctg ccgcctaatg atccgtattt catatctctt ctgaaacctt tcttcattgt
      181 cgatttttgt ccgcgtcgga caaggcacag aggccccctg cccgtcccgc cgttccccgt
      241 caccgtgctc ccatcgccct ctcgatgctt gcctgggcga tcttataatt ggacagagcg
      301 ctggtatagt tcgcctgggc cgtgacatac gtggcgaacg cgtcggagac ctcgatgggg
      361 cttcccacgc ctgctgcgta tctgccgttt gcgaggtcca tgttctcctt ggcctgatcg
      421 gctgcaagct gggctgttgc aatgctggcc tccgccgcct gcaggttcaa ataggcctgc
      481 ctgacatcga acatgatctg ctgccgcgta gactcttcat ttgccttcag gatatagaga
      541 ttggcttttg cctccgccac ctgatgactg gtcaggaaac cgctgaacaa ggggaccgtc
      601 aggaccacgc cggcgctcca accgtgattg agctgatagg gcgactggtc aatttccgcc
      661 caattgtagc tcgcgtttcc cgaaaggacg ggataatgcc ctgactgctg caggctgata
      721 ttcgattccg ccgcctgccg ttttgcaacg gcagacttgt agtcgggcct cttatccagg
      781 gcgtggctaa ccgcttcatc cagggttatc gtatatttct cgaatgtgag cttttcctcg
      841 atcgtatagg tcccctccgg cgcatcaggg actcccatga cattcttaag ggtgacccag
      901 gcgattttaa aagcattgtc tttattgatg agatccagtt tagcgttgct caggtctact
      961 tccgccttga tgacgtctat tctcgccttc gttccgacat cgtagaagcc cttcgcctgg
     1021 tcgaggtgca gttgaaactg tttaataacg tcctcagcga cgtcgcggtt cctcctggcc
     1081 tggaggacgc catagtaatc ctgtttgacg ctctggatga ccgaatcctg cgtcgattgc
     1141 aggtcatcgc gggatgcttc gagattatac ttcgaaacgt cgacttgcga ggaagtctta
     1201 ccgaaatcga ggatattctg gttgagcgtc acgctgctcg aaaactgatt aaacgtctca
     1261 ttgccgccga acgttgagga tcccggcact ggattgatcc tcgtataggc agccgatgcg
     1321 ctgagttgcg ggtaatacgc ggaacgcgcc tcacccaccc tgttcgagct cacgtctacc
     1381 ccgaaccgtg cggcaagaat cgtggggttt ttctcaagcg cgatctcaat tgcccgactg
     1441 agcgtcaggg tctcgaccga cgcggtcttg ccgcctccgg agcggtcatt atttttttcg
     1501 gattgtcccg ctgccggcaa cgggctgccc ggttgcgccg cctcttcccc gaaaagggga
     1561 tggagcggaa ctgtcccgaa taccagtgca gcgaagaccg ccagccccca tttcttcgat
     1621 cgatcaattc cccgcatatc gttctccccc atgccccttt ttatgatcgc agttcattgt
     1681 tccgtgggta taaatatcga tcatttcacg gatctccttc tccatgacgt gttttttcat
     1741 ccccgaggga cgcatgattt cttccgatct gaaataggaa aagagaatcc ataaaaacag
     1801 gcgggcggcc aattctgcgg acagattgct tctcaggatg ccgcgctgct tcaaatcctc
     1861 gaaataggcc gccagcgtcg ccctcatctc gtggataaac cggtcataga cctccttgat
     1921 cttgtccgga taactggtca cttcagaata catgatcttt atcagcgatt ttctctcttt
     1981 cagcgtcgtg agaaaacggg ttgcgatcaa gaccagggct tcttcacagg acattccctc
     2041 cagctcggga aggagttcct tcagcttcgg taaaaaagta taccttttca ggacctcttc
     2101 gaacagcaat tccttggacc cgaaatgcct gaacagggt
//
LOCUS       DASSLD010000294         5247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_362533,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000294 DASSLD010000000
VERSION     DASSLD010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362533"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..496)
                     /locus_tag="VFK23_05295"
     CDS             complement(116..496)
                     /locus_tag="VFK23_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318535.1"
                     /translation="MNERYKIRETKFFLAKMEESFHDRETFRYYLSAFLSAARSVTQY
                     AREESRLKGRLQWYEETIAGNDVLSHGSQTMVRNRFADWPGSEDRIGSSQRYSEDLIN
                     LSHRYIEELEKFVQAGIEEGILSG"
     gene            complement(514..897)
                     /locus_tag="VFK23_05300"
     CDS             complement(514..897)
                     /locus_tag="VFK23_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318536.1"
                     /translation="MKQLRVLMASAEELVEPSFRKAIEEGSHHLTILNPYEKKGEFLA
                     EAKSGGYDVIVLTNFGSPLDFSMGLISLLRLACKARVIVMSRVADRRKGPPRKGGVPF
                     YLFPLSRKQILKAVEKAAGMSELSR"
     gene            complement(1966..2127)
                     /locus_tag="VFK23_05305"
     CDS             complement(1966..2127)
                     /locus_tag="VFK23_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318537.1"
                     /translation="MLENRDLRSTDLPELPTYFLDVSSSGNLIMGASGTAQDTGTNNK
                     IFLQSSDGK"
     gene            complement(2229..2798)
                     /locus_tag="VFK23_05310"
     CDS             complement(2229..2798)
                     /locus_tag="VFK23_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318538.1"
                     /translation="MKKSFAITVVVPLSFAMFLVGVCIAGPPESTPTKPFVAQWTGTL
                     YNVGVCVPDTSKVLTVNFGQGSATLLGSASFVFMYCIDPITISGSGWGIVTTANGDKL
                     YERITNLTVTFGDPVAGTPTTWSEDEELLGGTGRFENARGDSKSSGIWTSAEGFPVGF
                     TVPPPMLLAIPPDTGVQNWIGTTKGEVRF"
     gene            complement(3018..4760)
                     /locus_tag="VFK23_05315"
     CDS             complement(3018..4760)
                     /locus_tag="VFK23_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318539.1"
                     /translation="MKKILCTYLPASLLLAFLAMILAACGGGGGGGDSGTTPTPTKSV
                     VITGTVPGTVAIVYELATGKEADRNVASGTPKTFSLSVAPGDYYLMFIENEGTNTQRS
                     FAFRNVTGGNVFTFKANTTLDLGVLVFNNYPRTAMPLIDRISGNDNVAESFKPEANFS
                     PGAGEWIETRKFVNSTCSGHSPGTAVTGNVKIAHGFGIVTYSPAETTETAIGVANVNT
                     AILTASAGALETIYLTMQSDGSLAGTYSKAGYGGECSEDATITAVLSTSPPPATTLTG
                     LSINGPSSMPEYGTAIYTATANWSDTSTSMVTPTWSVNSQIASISPFGVFSGQGGIAS
                     DQTVTIAATYSYGEITETATMSVTVTNFTGGISNPYLSRFEARLLSGEIFFEENVDAG
                     GMYESSLFVFNADSSFREYRYKNPPDTSEYVTGTWSIGASGEAILSYVGGKTFTWTLL
                     GWSFWTKMWVSVDDGTGTPSIVNLEISGPGPYPFNSSLIRGTYVNQYGDTWIFNSNGT
                     GSTTGSGGSTFTWSVDAGILKVFFPNGYVGWMYQLVSENTSTDYPVMEWAFVLNTPAD
                     DFHLYYGGMRLTRQ"
     gene            complement(4910..>5247)
                     /locus_tag="VFK23_05320"
     CDS             complement(4910..>5247)
                     /locus_tag="VFK23_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318540.1"
                     /translation="IVIRGTTVPEPLGLPVSRAHGRAMDSYVVRIYRRAGRKSRFLIG
                     TAEVAGTERKIAFSNIEELWEILRHREGRDLCAPPSPRRRLRKEVMSATAASDLEESA
                     EDVRQIKLT"
BASE COUNT         1183 a         1496 c         1343 g         1225 t
ORIGIN      
        1 tcagaaagcg tgcatcgatg acaaattcgg tctctgcctt cgcgatgggg ggagcaatgt
       61 atccgaccag aagaagaaca gtcaagatta cggtaagggc tctacgcata gcgcctcacc
      121 ccgataagat tccttcttca attcccgcct ggacgaactt ctccaactcc tcaatgtatc
      181 ggtgcgacaa attgatcagg tcttcagagt atcgttgcga cgaaccgatc cggtcttcag
      241 acccgggcca gtcagcgaac cgattccgga ccatcgtctg gctgccatga ctcaatacat
      301 cattcccggc gatagtctct tcataccatt ggagcctgcc cttcaacctt gattcctccc
      361 gggcatattg ggttacagac cgggccgcac tcaggaacgc gctcaagtag tatcggaacg
      421 tctcgcggtc gtgaaacgac tcttccatct tcgccaggaa gaacttggtc tcacgaatct
      481 tgtatcgctc gttcatctcg attcacgccc ccgctatcga gataattccg acatcccggc
      541 cgccttctcc acggccttaa gaatctgttt cctcgacaaa gggaacagat agaagggaac
      601 accgcctttc cggggaggac ccttccgtct gtccgccaca cgggacataa cgatcaccct
      661 cgctttacag gccaaacgca ggagagagat gagtcccatc gagaagtcca gcggggagcc
      721 gaaatttgtc aggacgatta catcgtaccc gcccgacttc gcctcggcga ggaactcccc
      781 cttcttctcg tagggattca aaatcgtgag atgatggctg ccttcttcga tggctttcct
      841 gaagctcggc tccacaagtt cctccgctga tgccatcagg acgcgcagtt gcttcatgaa
      901 cagcccccga gaaggaacat cgtaccgggt agtaatcgca ccctgtgcat cttttcggcg
      961 gttccgggtg gattcttcag gcaaaaaggg ccttaggtag aaaagtgggg aggctggatg
     1021 tatatacccc tgacttcggt cccctctgcg gctacgatca atactgctaa acgatagagg
     1081 cgatgcggag ggatttggat tttgaaaatg aagatgagga gggattttct ggtgatattc
     1141 cctcgaaacc gtgacgatac cctcgtgcgt acgaatcgat gtaagggtcc ccgctgccgc
     1201 caccagagtg gacgataagc aaagagtgct cttcggccct ttcatccgaa acaccgagaa
     1261 aatgaacctc tatccctcgc tgatatcctc ggagatactc agcccgcagc ggttctgaag
     1321 tatcgatctt ccggtcgatc attctgaaca gcgcctcgca aacattcttt gccaacatta
     1381 tccccccccc ttccggcatg ttctcttccc ttgggatatt cttcggcagt taagggcatt
     1441 atcttcaact gtttgacggc gagtaggtca gccggtataa ttcggtttca aacccttgca
     1501 gccatcctta tacccacgag cgtaagaatg aacgtcgcgg ctgccgtttc tacgggcgat
     1561 atcggccaat gaataggtat ccgggggtga tgccgactga gggttactga aatgaaattt
     1621 gatcccctct tgatatcccc tcccgtattc cactttcctc ggtgagcaac ctgtcatcat
     1681 tcgatctgcc agaaggaggc agttctggaa ttccgagtaa gtcattttcc ctcccacgcc
     1741 ccggcaatta ttaacttctt caaattcatt cggcagttac ggacaaaatc tttatgaaat
     1801 ttttagcggg cgaatgggat gaatagtggt gtgagtaaaa aagatgaggg gctacggttt
     1861 cccgtaaccc tgtctgttcg gacttccccg aacaaaaaaa gggcgggttt gcggtcccgc
     1921 cctctcgtca gccgaaggaa tcggccccgc gtcttagcgg cgtaactatt tcccgtcgga
     1981 gctctggagg aagatcttgt tgttggtgcc ggtgtcttgg gcggtcccgg acgcacccat
     2041 gatcaggtta cctgacgaag acacatccag aaagtaggtc gggagctccg gcaggtctgt
     2101 cgacctcaaa tcgcgattct cgagcattac gttattgctg gagctcaaga gcgcgaccaa
     2161 cggctctgtg tagtcgccct caaatggaat ctcatggcgc acctccatta agcgatgcga
     2221 gcgggagctt agaacctgac ctctccttta gtagtgccta tccagttctg gactcccgta
     2281 tccggcggta tcgcgagcaa cattggaggt ggaactgtaa acccaacggg aaacccttct
     2341 gccgatgtcc aaattcccga cgatttgcta tcaccacgtg cattctcgaa ccttcccgtc
     2401 ccaccgagga gttcctcatc ttctgaccat gtagtcgggg taccggcaac ggggtcaccg
     2461 aacgtgaccg tcaggtttgt aatacgttcg taaagcttat ctccattggc cgtggtgaca
     2521 atcccccatc cagaaccaga tatggtgatg gggtcgatgc aatacatgaa tacgaagctg
     2581 gccgaaccca ggagggtggc gctcccctgc ccgaagttaa cggtcagaac tttactggtg
     2641 tcaggaacac aaacacccac gttgtaaaga gtccctgtcc actgcgcaac aaagggtttc
     2701 gtaggtgtcg actctggggg acccgcaatg cataccccca cgaggaacat cgcaaaagac
     2761 aggggcacga ccactgtaat cgcgaaagat tttttcatcg tgaccctcct ttcaaggctc
     2821 taaggtgggt ttgttttgac ggactccctc ccaggaatcc tcgacgtctg acgtggccgt
     2881 cgcgctcatc acttccttaa aacggcgtcg cggagagggc agggcgcaaa gatcccggcc
     2941 cttccggtga agggggccgg atacgtccgc ccccttggtt tacggaaaga cgcctgattc
     3001 ccgggacgcc tgacccatca ttggcgcgtc agcctcatac ctccgtagta gaggtggaaa
     3061 tcatccgccg gcgtgttcaa cacaaaggcc cactccatga cagggtaatc cgtcgaagtg
     3121 ttttcgctca cgagttgata catccaacca acatatccgt tggggaagaa aaccttcaag
     3181 attccggcgt caacggacca tgtgaacgta gaccctccac tcccggtagt ggaccccgtc
     3241 ccgttggagt tgaaaatcca tgtgtcgcca tactggttga cgtaggtccc gcgaataagg
     3301 gaactgttaa acgggtatgg gcccggtccg ctgatctcca aattcacaat ggatggagtt
     3361 ccggttccat cgtcgaccga tacccacatc ttggtccaga acgaccaacc aaggagtgtc
     3421 cacgtaaaag tcttcccgcc gacgtagctc aggatcgctt ccccggacgc gccgatgctc
     3481 catgtcccgg tgacatattc gctcgtatca ggagggttct tgtacctgta ctccctgaac
     3541 gaggaatcgg cgttgaacac gaagagggag gactcataca tccctcccgc gtcgacattc
     3601 tcctcgaaga atatctcccc tgacagcaag cgggcttcga agcgggaaag ataaggattc
     3661 gagatgccgc ctgtaaagtt ggtgacggtt acactcattg tggccgtctc ggtgatttct
     3721 ccataggaat aggtcgctgc aatcgtcacc gtttgatcgc tggcgattcc cccctgaccg
     3781 gaaaagaccc cgaaagggct tattgatgca atctgggaat tgacactcca ggttggtgta
     3841 accatagatg ttgaagtgtc gctccagttc gccgtcgccg tgtatatggc ggtcccgtac
     3901 tcgggcatcg acgaggggcc gttgatggat agaccggtca aggtagtggc cggcgggggg
     3961 gaggtgctta gcaccgctgt aatcgtcgca tcttcagaac actctccgcc atatcctgct
     4021 ttgctatacg ttcccgcaag agaaccatcc gactgcatgg tcaaataaat ggtctccaag
     4081 gcaccagcgg aagcagtgag aatggccgta ttcacatttg cgacaccaat tgccgtctcg
     4141 gtcgtttcag caggtgaata agttacgata ccgaagccat gggctatctt tacattccct
     4201 gtaaccgctg ttccgggaga gtgaccggag caggtagagt ttacaaactt tcttgtttct
     4261 atccactctc cagcccccgg agagaaattt gcctcgggct tgaaggattc cgcaacattg
     4321 tcattcccgg atatgcggtc gatcaggggc atcgcagttc gaggatagtt attaaagaca
     4381 agaacaccga gatccagagt cgtgttcgct ttaaatgtga atacattccc tccagttaca
     4441 ttccggaaag cgaaacttct ctgggtgttg gtgccctcgt tctcgatgaa catcaggtaa
     4501 tagtctcccg gggccacact cagcgagaac gttttcggag tgccgctggc aacattccga
     4561 tcagcttctt tgcccgtggc aagctcatac acaatcgcca ctgtccctgg aaccgtaccc
     4621 gtgataacca cggattttgt gggtgtgggt gtcgtacccg aatcacctcc gccacctcct
     4681 cctccacacg cagcaaggat cattgccaga aaagctaaaa gcaatgaagc ggggagatat
     4741 gtgcataaga ttttcttcat ctcgatttcc ctccttcctg cccttctcgg cccctttctc
     4801 tcaagttggc tctcaggagg ggcggccctc agacgcttcc gccgcttctc ccgaaccggt
     4861 taagttgtgc gaacggggtc ttctcatcga attcctcttt tcaagatcct taggtgagtt
     4921 tgatttgacg gacatcctcc gcggattcct cgaggtctga ggcggccgtc gcgctcatca
     4981 cctccttccg taaacggcgt cgcggagacg gcggggcgca aagatcccgg ccctcccggt
     5041 gcctgaggat ttcccatagc tcctcgatat tcgagaaggc tatcttcctc tcagtacccg
     5101 ccacctcggc cgtcccgatc aaaaagcggg actttcttcc ggcgcggcgg tagattcgaa
     5161 cgacgtagct gtccatggct ctcccatgcg cgcggctgac cggaagaccc aaaggttccg
     5221 gcacggtagt ccctctgatc acaatag
//
LOCUS       DASSLD010000295         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1077929, whole genome shotgun sequence.
ACCESSION   DASSLD010000295 DASSLD010000000
VERSION     DASSLD010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077929"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /gene="mltG"
                     /locus_tag="VFK23_05325"
     CDS             complement(<1..671)
                     /gene="mltG"
                     /locus_tag="VFK23_05325"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET7318541.1"
                     /translation="MKRGGNWWTRHPWRSFLLATASLLFVIHFYIALFVPPSKEKVWK
                     EIEITEGMTFKAITAMLQQEGIIRYRGYFVIIGRLQGLSRKVRTGYYGLSTNMTLWDV
                     LDALRKGKIIEYEVLIPEGYNLYQIGWTLSGTPLVTDPDKFFRLVKDKNYVHSLGIEA
                     DSLEGYLFPDTYYLPKGIKLEDIPKKMVQRYREMFTDSLRRRAADLGFTEHQILTLAS
                     IVEKEA"
     gene            complement(737..>2284)
                     /locus_tag="VFK23_05330"
     CDS             complement(737..>2284)
                     /locus_tag="VFK23_05330"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET7318542.1"
                     /translation="PSPFVPPIGAPERLRLMFENLGPTFIKFGQVLACRPDMLPLEYS
                     REFLKLTDSVAPFPSVEARRIIEEDLKAPIDRIFTSFDDRPVAAASIAQVHRAELPDG
                     SEVMVKVQRPHIDRTIERDISIMRWLAELIVARVPEMAPYDIPGIVDEFARTIRRELD
                     FFIEASNAVQLRKNFESSAVLYVPRVFTDISSKRILVMEEIEGIRIDEYARLDREGYD
                     RKDVARKGGAAFFKMVLQDGLFHADPHPGNIFVLPDGRLGLVDFGIMGRVTEENMEYF
                     ASIFLALASHDYDALVRVYLDMGFLPAETADIEKFQREMKEDLVELLEPYYVMQVKQI
                     DFGAYIDRVTQILIRHQMKLPSNLYLMDKALITLEGILKQLDPEFNYFEAAEPYAAEL
                     VRRRRNPLKVAQNVKKNMEEFVDAFALLPKQVRTSFRQLLHGDFRMNLHHEDLGRLIR
                     DIDKSSNRLAFSVITASIIVASSIIIHSGQGPMLFGLPMFGLIGYVIAAFLGFWILIG
                     ILKSGQL"
BASE COUNT          475 a          653 c          663 g          493 t
ORIGIN      
        1 gcttccttct ccacgatcga ggcgagggta agaatctggt gctcggtaaa gcccagatct
       61 gccgcacgtc gtcttaagct gtccgtgaac atttcccggt agcgctggac catctttttc
      121 gggatatctt cgagcttgat gcccttgggc agataatagg tgtccgggaa caggtagcct
      181 tcaagcgagt cggcctcgat gccgagggaa tggacgtaat tcttgtcctt caccagcctg
      241 aaaaacttgt ccgggtcggt gacgagcggc gtgccggaaa gcgtccagcc gatctggtac
      301 agattatacc cctccggtat caggacctcg tactcgatga tcttgccttt gcgaagggca
      361 tccagcacgt cccacagggt catgttcgtg ctcaggccgt agtaaccggt acgaactttg
      421 cggctgagac cctggagcct gccgatgatg acgaaatatc cgcggtagcg gatgatccct
      481 tcctgctgca gcattgccgt gatggccttg aaggtcatgc cctcggtgat ctcgatttcc
      541 ttccagacct tctctttcga cggcggcacg aacaacgcta tgtagaaatg gatgacgaac
      601 agcagcgatg cggtcgccaa aagaaacgat cgccacggat gcctcgtcca ccagtttccg
      661 ccacgcttca tacacttctc cgcattcatc ctgcatccgc atattgcaag agcaggatga
      721 aatcagaatc catgcttcac agctgccccg atttcaggat cccgatcagg atccagaaac
      781 ccaggaacgc tgctataaca tacccgatta ggccgaacat aggcaagccg aaaagcatgg
      841 ggccctgccc ggagtggatg atgatggacg atgccacgat gattgacgcg gtgatgacgc
      901 tgaaggcgag gcggttgctt gatttatcga tgtccctgat caggcggccc agatcctcgt
      961 ggtgcaggtt catccggaaa tcgccgtgca ggagctgcct gaacgacgtc cttacctgct
     1021 tcggcagcag tgcaaaggcg tcgacaaact cctccatatt ttttttcacg ttttgcgcga
     1081 ccttgagcgg gttccgtctg cgacggacca gctccgccgc atagggctcc gccgcttcga
     1141 agtagttgaa ctccggatcg agctgcttca ggatcccctc gagggtgatg agcgccttgt
     1201 ccatcaggta gaggttagaa ggaagtttca tctgatgccg gatgagaatc tgggtcaccc
     1261 ggtcgatgta ggcgccgaag tcgatctgtt tcacctgcat gacgtagtag ggttccagga
     1321 gctcgacgag gtcctccttc atctcccgtt ggaatttctc gatgtcggct gtttccgcgg
     1381 gaaggaagcc catgtccagg tacacgcgta ccagagcgtc ataatcatgg cttgccaggg
     1441 cgagaaagat ggacgcaaaa tattccatgt tctcttccgt gacgcggccc atgatgccga
     1501 agtcgaccag gccgagcctg ccgtcgggca gcacgaaaat attgcccgga tgaggatcgg
     1561 catggaacaa gccgtcctgc agaaccatct tgaagaatgc ggcgccccct ttccgggcaa
     1621 cgtccttgcg gtcataccct tcccggtcga ggcgcgcgta ttcgtcgatc cggatgccct
     1681 cgatctcttc catgacgagg atccgcttgc tggaaatgtc ggtaaacacc cgggggacgt
     1741 acaggacggc gctcgattcg aaattcttcc gcagctggac ggcgttcgag gcttctatga
     1801 agaaatcgag ctcccgtctg atggtgcggg caaattcatc gacgatcccc gggatgtcgt
     1861 acggcgccat ttccggcacc ctcgccacga tcagttcggc gagccatctc atgatgctga
     1921 tgtcccgttc gatcgtgcgg tcgatgtggg gccgctgcac cttgaccatc acttcgctcc
     1981 cgtcaggaag ctcggcccgg tggacctggg caatggacgc ggctgccacc ggccggtcgt
     2041 cgaacgacgt gaagatacgg tcgatcggcg ccttgagatc ttcctcgatg atcctcctcg
     2101 cctccacgga aggaaaaggc gctacggagt cggtcagttt cagaaattca cgggagtatt
     2161 cgaggggcag catgtccggc cggcatgcca gcacctggcc gaacttgatg aaggtggggc
     2221 cgagattttc gaacatcagc cggagacgct ccggcgcgcc tataggcggg acaaagggcg
     2281 aggg
//
LOCUS       DASSLD010000296         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1585039, whole genome shotgun sequence.
ACCESSION   DASSLD010000296 DASSLD010000000
VERSION     DASSLD010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585039"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="VFK23_05335"
     CDS             <1..1168
                     /locus_tag="VFK23_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7318543.1"
                     /translation="KGIITVLSEEPDTPYFRPMIPYVVTGKKKAENIGLSGNGPYTAA
                     GIEVRTGAKVDSVDAARKLVSINGRDTLSYDKLLLATGSSPYIPPELEGTNAEGVFTL
                     RKLSDARSMAQRAQNTDHAVMLGGGLLNLKAAFALLELGIKVTLVVFSPEVLSQLMEP
                     EDAGLIRHALDKAGLKIITGHSAKGVVSDKSGVTGVCLDDGSEIPCQMLCIGKGVRPN
                     INFLSGSAIATDKGVVVDRYTVCSDPHAFAAGDVAVTFDPVTGKRIMTALWTNAVEMG
                     RCAGHNMAGAKTAYTGTFGIMNATQVADMPFVSMGIVHTRGTDYEVHVSATGTTYRKV
                     VFSSDGERLVGVLFIGDITHAGMYRYLIRENRQISGIKSHIVDQTLHYGHLLKG"
     gene            complement(1219..>2715)
                     /locus_tag="VFK23_05340"
     CDS             complement(1219..>2715)
                     /locus_tag="VFK23_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HET7318544.1"
                     /translation="YDPLLSDKEKLFEIIEELYNFWRRFERFIYLEAPQRSAYAKNSL
                     HHAQFIKSNEEFKNLVLFVYRRISEHLIGKNPRVYRQLPAGANMGMLLEKAVWNCPER
                     YGILRNIPFIRLTLVEPPVILYPVMNKRKGRFEEVRDLSPEMLSLNPEEWYCYPAKVG
                     DLTAFIYFHHDFTSLALSLCNLFEIADYEDIEGRTPDIVLLFGLESGSLPGDTLFYED
                     KAGNMLVGLVRHSEEVDYFGYFKKMTLTLHNIAMLKRNRLPLHGAMVCIKLKDGGSAN
                     VVIIGDSGAGKSETIEALRTMADEYICEMTIIFDDMGSLGIDGSGRVAGYGTEIGAFV
                     RLDDLQSGYAYEEIDRSIFMNPDKSNARLIMPITRYHHIVKGYPVDMFLYANNYEQVN
                     DDVPAIEFFATKEEAFPTFRDAARLAKGTTDEKGLVRTYFANPFGAPQRKEDHERIAR
                     YFFERMFESGVRVGQIRTRLGISGFEQAGPKAASMALFQEIRKLCRAK"
BASE COUNT          587 a          720 c          806 g          602 t
ORIGIN      
        1 gaaaggcatt atcacggtat tgtccgaaga gccggatacg ccgtatttca ggccgatgat
       61 accctatgtc gtaaccggca aaaagaaggc tgagaacata gggctgtcag gcaacggtcc
      121 ctacacggcc gcaggcatcg aggtccgcac cggagcgaaa gttgactctg tggatgccgc
      181 gcggaaattg gtaagcataa acggcaggga cacgttgtcc tatgacaagc ttttattggc
      241 gaccggcagc agtccatata ttcccccgga gctggaggga acaaatgcag agggtgtctt
      301 tactcttcgg aaattaagcg atgcgagatc tatggctcag agagcccaaa acacggacca
      361 cgccgtaatg ctgggaggag gacttctcaa tctcaaggct gcttttgccc ttctggagct
      421 gggaataaag gtcaccctcg tcgttttctc tcccgaggtc ctgtctcagc tcatggaacc
      481 ggaagatgcc ggactgatcc gccatgccct tgacaaagcg ggattgaaga taataaccgg
      541 gcacagcgcc aagggtgttg tctcggacaa aagcggcgtg acaggggtct gccttgatga
      601 cgggtccgag ataccttgtc agatgctctg tattggaaaa ggggtgaggc cgaacatcaa
      661 tttcctgtcc ggaagcgcca tagcaaccga caagggtgtc gtagtggaca gatatacggt
      721 ctgcagtgat ccccatgcct ttgccgccgg cgacgtggcc gtgacctttg atcccgtgac
      781 gggaaaaagg atcatgaccg cgctctggac aaacgccgtc gagatgggca ggtgcgccgg
      841 ccataacatg gcgggcgcaa agaccgccta taccggcacc ttcgggatca tgaacgcgac
      901 acaggttgcc gacatgcctt ttgtctcgat gggtattgtt catacgaggg gtactgacta
      961 tgaagtgcat gtgagcgcca ccggaacaac ctatcgcaag gtggtgttca gctctgacgg
     1021 cgaaaggctc gtcggtgtgc tttttatagg cgacattacc catgccggga tgtatcgata
     1081 tctgatacgg gagaacaggc aaatcagcgg gatcaagtca catatcgtgg atcagacgct
     1141 gcattacggc catcttctca aaggctgaca agcagcccct tgagtcgaag ttgagacggt
     1201 aggtctgaga aatcaccgtt atttcgcgcg gcaaagcttc ctgatttcct gaaagagggc
     1261 catcgaggct gccttcggtc cggcctgttc gaagccgcta atgccgaggc gcgtccgtat
     1321 ctgtcccacc ctgaccccgc tctcgaacat ccgttcgaaa aagtacctcg cgatcctttc
     1381 gtggtcctct tttctctgcg gcgcgccgaa gggattcgcg aaataggtgc gcaccaggcc
     1441 cttttcgtcc gtcgtgcctt ttgcaaggcg cgcggcgtcc ctgaacgtgg gaaaagcctc
     1501 ctctttcgtc gcaaagaact cgatggccgg cacgtcgtca ttcacctgct cgtagttgtt
     1561 cgcgtagagg aacatgtcca ccggataacc cttgacaata tggtggtacc gggtgatggg
     1621 catgatgagt cttgcgttgg acttgtcggg gttcatgaag atgctgcggt cgatttcctc
     1681 gtaggcataa cctgattgaa ggtcatcgag ccgaacgaag gcgccgatct ccgttccgta
     1741 gcccgccacc cggccgctcc cgtcgatccc gagagagccc atgtcgtcga agatgatggt
     1801 catttcacaa atgtattcgt cggccatcgt gcgcagggcc tcaatcgtct cggatttgcc
     1861 cgccccgctg tccccgatga tgacgacgtt cgcggaaccg ccgtccttga gcttgatgca
     1921 taccatggcg ccgtgcagcg gaaggcggtt tcgcttgagc atggcgatgt tgtggagcgt
     1981 cagggtcatc ttcttgaagt atccgaaata atcgacctcc tccgaatggc ggacaagacc
     2041 cacgagcatg ttgccggcct tgtcttcata gaagagcgtg tcgcccggga gggagccgct
     2101 ttccaggccg aacagcagga cgatatccgg ggtccttcct tctatatcct cgtaatcggc
     2161 gatttcgaag aggttgcaca gggaaagcgc cagggatgtg aaatcgtgat ggaagtagat
     2221 gaatgccgtc aggtccccga ccttggccgg atagcaatac cattcttcgg ggttgaggga
     2281 aagcatttcc ggactgaggt cgcggacctc ttcgaaccgg ccttttctct tgttcatgac
     2341 cgggtagagg atcacgggcg gttcgacgag ggtgagccgt ataaagggga tgttccgcag
     2401 gatgccgtat cgttccgggc aattccagac cgccttttcg agcagcatgc ccatgttcgc
     2461 gcccgcgggg agctggcggt acacgcgcgg attcttgccg atcaggtgtt cactgatgcg
     2521 gcggtacaca aagagcacga ggttcttgaa ttcctcgttc gatttgatga actgggcgtg
     2581 gtggaggctg ttctttgcgt aggccgaacg ctgcggcgct tcaagataga tgaagcgttc
     2641 gaaccgccgc cagaagttgt acaattcctc gatgatctcg aagagcttct ccttgtccga
     2701 aaggaggggg tcgta
//
LOCUS       DASSLD010000297         3373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_332020,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000297 DASSLD010000000
VERSION     DASSLD010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332020"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VFK23_05345"
     CDS             complement(<1..1193)
                     /locus_tag="VFK23_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET7318545.1"
                     /translation="MAKLSAEAKVGLLVVVSSVILLWMTMMVGKFEFGKPKGYRVAAV
                     FETISGLDLKAAVRMAGVQIGTVESVELVKNKAKVVLRINPDVKIPRETEAAVKTMGL
                     LGDKYVDLIPPPQAEPGMEAKERGYYRDGDVIERSVPPSDADQLIRKFSGIADDVKQI
                     TASLRQVLGSEKGTKSMEDILEDLRQTTANVKEFSQTLKSDGSELVVSMNELVASLNG
                     VVGENRDNLRVTMENVKEASKNAEMAMASLDNTIKKIDKGEGTIGKLVNNDSMYNSID
                     SAAKGLTDYVSRIERMKTIVGFRSEYMFPQSKTYASLELKPRPDTSYILELTSDPFGN
                     FKRVEQTTTPPGGTVVTETFEDKFKFSLEYAKRWGNLAVRLGLIESTGGIGADYYVWD
                     DRFKF"
     gene            complement(1315..2073)
                     /locus_tag="VFK23_05350"
     CDS             complement(1315..2073)
                     /locus_tag="VFK23_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7318546.1"
                     /translation="MAEPMIEITNLHKNFGSQHVLRGVNLTVEKGESMTVIGGSGSGK
                     SVLIKHIIGLLFPDQGQVKVAGQVLNDLDEHHLNELRKKFGMLFQMAALFDSLTVWEN
                     VGFGLKQHTKLSDQEIRKIATEKLALVGLKDVEDKMPVDLSGGMRKRVGLARAIAMDA
                     EIILYDEPTTGLDPITADSINDLIVDLKKKLGVTSVAITHDMQSAYKISDRIAMLYKG
                     QIIETGTPDEIRSSQNPIVHQFITGRAVGPITAE"
     gene            complement(2077..2793)
                     /locus_tag="VFK23_05355"
     CDS             complement(2077..2793)
                     /locus_tag="VFK23_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7318547.1"
                     /translation="MGGVLLFLGQTLRWCVTPPFYLKNLLKQMEQIGVNSVPVVLTTA
                     ISTGMVLALQSYTGFKRFGAQSLIGAVVSLSMTRELGPVLTGLMVAGRAGASMAAELG
                     TMRVTEQIDALYTMATNPMKYLVVPRFLASTIMMLFLSALGMFIGVLGGYFVGVKVLE
                     TNPVTYMNQSINNTELQDIWYGLVKSLVFGAVVGLIGCYKGFNTEGGAEGVGKATTGA
                     VVVSCMLILILDYFLSALLW"
     gene            complement(2840..>3373)
                     /gene="alr"
                     /locus_tag="VFK23_05360"
     CDS             complement(2840..>3373)
                     /gene="alr"
                     /locus_tag="VFK23_05360"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET7318548.1"
                     /translation="SPVPLRHASNSAAVLDFGRALFTMVRPGLMLYGYNPLEQAAGGA
                     DLRPALSFVTRVAFLKKVKAGVPISYGRTFVTKRESIIATLPVGYADGFSRALSNRGE
                     ALVRGKRVPVAGRVCMDMCMIDVTDVPGVQEGDEVVLIGSQSGERITADDIAAKIGTI
                     PYEVLCGIGSRVPRIYH"
BASE COUNT          606 a         1069 c          947 g          751 t
ORIGIN      
        1 ctgaacttga accggtcgtc ccagacgtaa taatctgccc cgatgccgcc tgtcgattcg
       61 atcagcccta gccggaccgc aaggttcccc caccgcttcg cgtactcgag gctgaacttg
      121 aacttgtcct cgaaggtctc cgtcacgacc gttccaccgg ggggcgtcgt cgtctgttcc
      181 acccgcttga agttgccgaa ggggtcggac gtcaattcga ggatatagga cgtgtccgga
      241 cggggcttga gctccaacga ggcgtaggtc tttgactggg ggaacatgta ctcgctccgg
      301 aatccgacga tcgtcttcat gcgctcgatg cgcgacacgt agtccgtgag ccccttggcg
      361 gcgctgtcga tgctgttgta catgctgtcg ttgttgacca gcttgccgat cgtgccctct
      421 cccttgtcga tcttttttat ggtattgtcg agggaggcca tggccatctc ggcgtttttc
      481 gacgcctcct tgacgttctc catggtcacc cgcagattgt cccggttctc ccccacgacc
      541 ccgttgaggc ttgcaaccaa ttcgttcatg ctgacgacga gttcgctgcc gtcgctcttg
      601 agggtctggg aaaattcctt cacattcgcc gtggtctggc gcaggtcttc aaggatgtcc
      661 tccatggact tcgtgccctt ctcggagccc aggacctgcc ggagcgacgc cgtgatctgt
      721 ttgacgtcat cggcgattcc cgagaatttt ctgatcagtt ggtctgcgtc gctggggggc
      781 accgaccgtt cgatgacgtc tccgtctctg taatagcccc tctctttcgc ctccatgccc
      841 ggttcagcct gcggcggggg gatcaggtcg acatacttgt ccccgaggag gcccatggtc
      901 ttgaccgcgg cttctgtctc ccgcggaatc ttgacgtcgg gattgatgcg gagcacgact
      961 tttgccttgt ttttgaccag ctccacggac tctacggtcc cgatctgaac accggccatg
     1021 cggactgcgg ccttgagatc gaggccggaa atagtctcga acacagctgc aacgcgatag
     1081 cccttcggct tgccgaattc gaacttgccc accatcatgg tcatccacag caggatgacc
     1141 gagctcacca caaccagcag gccgaccttt gcttctgcgc tcagttttgc catgcaaact
     1201 cctcgtagca cttcgtactt cgcatatcgc atggcgctta gcgtaaagat tctacactat
     1261 gctctttgca atttacgctc tacgctttgc gctatgcctt ttgcgagttt cgaatcactc
     1321 cgccgttatc ggccccaccg cgcgacccgt gatgaactgg tgaacgatgg ggttctgaga
     1381 acttctgatt tcgtccggcg tacccgtttc gatgatctgc cccttgtaca gcatggcgat
     1441 ccggtccgat atcttgtagg cgctctgcat gtcgtgcgtg atcgccactg acgtaacacc
     1501 aagtttcttt ttgagatcga cgatcaggtc gttgatcgag tcggccgtaa tggggtccag
     1561 gcccgtcgta ggctcgtcat agagaatgat ctccgcgtcc atggcgatgg cgcgtgcaag
     1621 ccccacgcgc ttgcgcatgc cgcctgacag gtcgacgggc atcttgtcct cgacatcctt
     1681 caggccgacg agcgccagtt tctcggtcgc gattttcctg atctcctggt ccgacaattt
     1741 tgtgtgctgt ttcaggccga aaccgacatt ctcccacacc gtgagcgaat cgaagagcgc
     1801 cgccatctgg aacagcatgc cgaacttttt tcgcagttcg ttcaaatggt gttcgtcgag
     1861 atcgttcagg acctggcctg ccaccttgac ctggccctgg tcggggaaga gcaggccgat
     1921 gatgtgtttg atcagcacgc tcttgccgct gccgctgccg ccgatgacgg tcatgctctc
     1981 acccttttcg acggtcaggt tcacaccccg gagcacatgc tggctgccga agttcttgtg
     2041 caggttggtg atctcgatca tcggttcagc cataggtcac cacagcagcg ccgacaggaa
     2101 gtaatcgagg atcaggatca gcatgcacga gacgaccacg gcaccggtcg ttgcctttcc
     2161 cacgccctcg gcgccgccct cggtgttgaa gcccttgtag catccgatga gccccaccac
     2221 ggcgccgaaa accagggatt tgacgaggcc gtaccagata tcctgcaact cggtgttgtt
     2281 gatactctgg ttcatgtacg tcacggggtt ggtctccagc accttgactc ccacgaagta
     2341 cccgcccagg acaccgatga acatgcccag ggccgaaaga aagagcatca tgatggtgct
     2401 tgccaaaaac cgcggcacga ccaggtactt catcgggttc gtggccatgg tatagagcgc
     2461 gtcgatctgc tcggtcacgc gcatggtgcc cagttccgcg gccatggacg ctcccgccct
     2521 gcccgccacc ataaggccgg tcaacacggg gccgagttcc cgcgtcatgg aaagcgatac
     2581 gacggcgccg atgagcgact gtgctccgaa gcgcttgaaa cccgtgtagc tctggagcgc
     2641 cagcaccatg cccgtcgata tggccgtcgt gagcacgacc gggacggaat taacgccgat
     2701 ctgctccatc tgcttcagca ggtttttcag gtaaaaggga ggggtcacgc accagcggag
     2761 cgtctgtccc agaaacagca ggacgccccc cacctcggag attaatcccg acgtcttact
     2821 gccaacctgt tcaagcaggt taatgataga tccttggaac ccggctgccg atgccacaga
     2881 gcacctcata ggggatcgtc ccgatcttcg ccgcgatgtc gtcggcggtg atgcgttcgc
     2941 ctgactggct gccgatgagg acgacctcgt cgccctcctg gacgcccggg acgtccgtca
     3001 cgtcgatcat gcacatgtcc atgcacaccc tgcccgcaac cggcaccctc tttccccgca
     3061 cgagcgcttc gcctcggttc gaaagagcgc ggctgaagcc gtcggcgtac ccgaccggca
     3121 gcgtggcgat gatgctctcc cgcttcgtga cgaaggtcct gccgtagctg atcggaacgc
     3181 cggccttcac cttcttcagg aacgcgacgc gcgttacaaa agacagcgcc ggcctcaggt
     3241 ccgcgccccc tgccgcctgt tcgagcgggt tgtacccgta cagcatcagt cccggcctga
     3301 ccatggtgaa cagcgcgcgc ccgaaatcga ggaccgccgc gctgttggag gcgtggcgca
     3361 gcggcacggg gct
//
LOCUS       DASSLD010000298         3877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_634707,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000298 DASSLD010000000
VERSION     DASSLD010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634707"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFK23_05365"
     CDS             <1..196
                     /locus_tag="VFK23_05365"
                     /inference="COORDINATES: protein motif:HMM:NF037182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318549.1"
                     /translation="ETVLLGPAPAPIARVRGQYRFQLLLLWTRREAIRRLAADARNIV
                     EEKYGRKVRVIVDVDPVNLM"
     gene            314..649
                     /locus_tag="VFK23_05370"
     CDS             314..649
                     /locus_tag="VFK23_05370"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET7318550.1"
                     /translation="MVKISFYQTLSGRSPAEEFLLALPEETRLEIADALVLLESGHRL
                     EMPLSRSLSGIRPGLHELRFRDRAGQVRVIYFIKKEDAIYLIHGFRKKTRTIPRRELD
                     IIHQRLKEI"
     gene            652..933
                     /locus_tag="VFK23_05375"
     CDS             652..933
                     /locus_tag="VFK23_05375"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7318551.1"
                     /translation="MAWKEISIEELAKSLGANVTEIREKQRLVGLIVKARKAKKLSQT
                     ALAKKLGISQARIAQIESGVGTAKVTFDVLFNMLVALGYDYRVVTKKAA"
     gene            1122..1589
                     /gene="smpB"
                     /locus_tag="VFK23_05380"
     CDS             1122..1589
                     /gene="smpB"
                     /locus_tag="VFK23_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018707757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET7318552.1"
                     /translation="MTEKKQDTAVATNRKAYHDYFVEETLEAGLVLQGTEVKSLRLGL
                     ANLTDSYAIVKNNEVFLFNANISPYPHGNIMNHEPLRTRKLLLHAEEIRKLIGKMTQK
                     GFTLIPLKIYFVRGKAKVLIGLAKGKKAFDKRETIKEKESKREVERVIKERSR"
     gene            1795..2562
                     /locus_tag="VFK23_05385"
     CDS             1795..2562
                     /locus_tag="VFK23_05385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7318553.1"
                     /translation="MAATLGSTAAHAKVTGKDVEYTAGGVTLKGYLAYDTAKKGKGPG
                     VLVVHEWWGLNDYARERARMLAKLGYTALAVDMYGEGKTATHPDDAGKFSSELMQNFD
                     TAKARFLAAEDFLKMQPTVDPGRIAAIGYCFGGGVVLNMARQGADLLAVASFHGNLTA
                     IKPAEPGMVKAKIRVYHGADDKFVTPEAVEAFNKEMTDAKADFRFIAYPGAVHSFTNP
                     EATAYGKKFNLPLAYDKKADRESWADMKKFFAETLKK"
     gene            2637..3116
                     /locus_tag="VFK23_05390"
     CDS             2637..3116
                     /locus_tag="VFK23_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7318554.1"
                     /translation="MKANSNSRPPFPPFSLETATQKVRMAEDAWNSRDPERVSLAYTV
                     DSVWRNRVEFFSGRDEIVAFLRRKWAKELDYRLIKELWAFRESRIAVRFAYEWHDDSG
                     NWFRSYGNENWEFDENGLMRRRIASINDLPIGETDRKYHWPLGRRPDDHPGLSELGL"
     gene            3139..3843
                     /locus_tag="VFK23_05395"
                     /pseudo
     CDS             3139..3843
                     /locus_tag="VFK23_05395"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
BASE COUNT          958 a         1050 c         1127 g          742 t
ORIGIN      
        1 ggagaccgtg ctcctgggcc ccgcgccggc gccgattgcc cgggtgcgcg gccagtaccg
       61 gttccagctc ctgctcctgt ggacgaggcg cgaagcgatc cgcaggctcg ctgcggacgc
      121 acggaacatc gtcgaggaga agtacggcag gaaggtaagg gtgattgtcg atgtagaccc
      181 ggtgaatttg atgtaggttt gtcgcagcac tcttactcaa acagcatctt tacagggggc
      241 gaccgatgcc tccctttgaa gccggccggt caaaataagc cttgccttat tattagttat
      301 aacttataat cccgtggtga agatcagttt ctaccaaacg ctcagcggca gaagccctgc
      361 cgaggagttt ctgcttgcct tacccgagga aacacgcctt gagatcgctg atgcgctggt
      421 tcttcttgag agcggacaca gactggaaat gccgctgagc cggagcctct ccggcattcg
      481 gcccggcttg cacgaactgc gattccgcga tagagcaggc caggtgcgtg tgatttattt
      541 tatcaagaaa gaggatgcaa tctacttgat tcacggattt cgcaagaaga cacggaccat
      601 tccccgacgg gaattggata tcattcatca aagactgaag gagatttgat catggcttgg
      661 aaggaaattt ccatcgagga actggcaaag tccctcggag cgaatgttac cgagatccgg
      721 gagaaacagc ggctcgtcgg attgatcgtg aaggcccgga aagcaaagaa gctgtctcag
      781 actgcccttg ccaaaaaact cggaatcagc caggcgagaa tcgcccagat tgaatccggc
      841 gtcggcacag cgaaagtcac ctttgacgtg ttgttcaaca tgctggtcgc gctgggatat
      901 gattatcgag tggtaacaaa aaaagcggcc tgacgtcagg ccgttcctca cgcaaataca
      961 ccgttatcat tgacatcgcc gtgagtcagg aatatattcg tcacgatcaa aaaaaggaag
     1021 gcgcgtttgt atacccttga gaagcttttc gattaagcaa cattcatatg tcgaagacac
     1081 gtacctttta tcagcaaggc aataaaagca gtaaatagac tatgaccgag aaaaaacagg
     1141 acaccgcggt cgccacgaac cgcaaggctt accacgacta cttcgtcgag gagaccctcg
     1201 aggcggggct cgtactccag ggcacggagg tgaaatccct tcgcctgggg ctcgcaaacc
     1261 tgacggacag ctacgccatc gtgaagaaca acgaggtgtt tctcttcaac gcgaacatca
     1321 gcccctaccc ccacggcaac atcatgaacc acgaaccgct ccggacgcgc aaactcctcc
     1381 tgcatgcgga agagatcaga aagctcatcg gtaagatgac gcagaagggc ttcaccctca
     1441 tcccgctcaa gatctatttc gtgcgcggca aggccaaggt cctgatcggc ctggccaagg
     1501 gcaaaaaggc cttcgataag cgcgaaacga tcaaggagaa ggaatcgaag cgggaagtcg
     1561 agcgcgtgat aaaagagcgg agcaggtagc cgtcgcattc tttcattttc cgtctgctac
     1621 aattaatgta tctacctaga tcgtcattcc cgcgaaagcg ggaatccatt ggctcttcta
     1681 aaaaacaaat tcaaaatgga ttcccgatag gagcattcgg gaatgacgaa caagattcac
     1741 accaaggagg cagctatgag acgatttctg gcgatttttg caggcgcgat ttttatggca
     1801 gcgactcttg gctcgacagc ggcccatgcc aaggtgacgg ggaaggatgt cgaatacacg
     1861 gccggcggtg tgacgctcaa gggctacctt gcctatgaca cggcaaagaa aggcaaaggc
     1921 cccggcgtcc tcgtggtcca cgagtggtgg ggcctgaacg attacgcccg ggagcgcgca
     1981 cggatgctgg ccaaactcgg atacacggcc ctggccgtgg acatgtacgg agaggggaag
     2041 actgcgactc atcccgatga tgcagggaaa ttctcctcgg aactgatgca gaacttcgac
     2101 acggcaaagg cacggttcct ggcagcggag gactttctca agatgcagcc gaccgtcgat
     2161 cccggacgca tcgcggcaat aggctattgc tttggcggcg gcgtggtcct gaacatggcg
     2221 cggcaggggg cggacctctt ggcggtcgcg agcttccacg ggaacctgac ggcgatcaaa
     2281 cccgctgaac ccggcatggt caaggcgaaa atcagggtct accacggagc cgacgacaaa
     2341 tttgtcaccc ccgaggcggt cgaggcgttc aataaagaga tgaccgatgc caaggcggat
     2401 ttccggttca tcgcctatcc cggcgcggtg cacagcttta ccaatcccga ggcaaccgcc
     2461 tatgggaaga agttcaacct cccgctcgcc tatgacaaga aggcggaccg ggagtcgtgg
     2521 gcggacatga agaagttctt cgcggaaacg ctgaagaagt aactttcgaa agacgggcgc
     2581 agggtgccgt ttcggacctg cgcccggagt ttgtttcccg tcatgtgagg tgcgttatga
     2641 aagcgaactc gaacagccgg ccgcctttcc cgccgttcag cctcgaaacc gcgactcaaa
     2701 aagtgcgcat ggccgaagac gcctggaact cccgtgatcc cgagcgggta tcgctcgcct
     2761 acaccgtgga cagcgtgtgg cgcaaccgcg tcgaattctt ctcgggccgt gacgagatcg
     2821 tcgcgttcct caggcgcaaa tgggcgaagg agctcgacta ccggctcatc aaggagctct
     2881 gggcttttcg cgaatcgcgc atagcggtgc gctttgctta cgagtggcat gacgactcgg
     2941 gcaactggtt ccgttcctac ggcaacgaga actgggagtt cgacgagaac ggcctcatgc
     3001 ggcggcgcat cgcgagcatc aacgacctgc ctatcgggga gacggaccgc aaataccact
     3061 ggcccctggg acggaggccg gacgatcatc cgggcctgtc ggaactcgga ctctgagact
     3121 gctccaaacc ggcatcccat gaatgcgcgc gatatcaaaa agcggctccg cttgctcgcc
     3181 gataaagaga aggctaaggt ccttcagggc tttttcaaga cgggcccggg acagtacgga
     3241 gagggcgacg tgtttctcgg gatcacggtg cccgcgctta gaagactggc gaagcaatgc
     3301 agggacacgt ccgtcgccga agccgcgcgg ttacttgcgt cccctgtcca tgaagaacgg
     3361 ctgctcgcgc ttttgctgct cgtcaggatt tttgaggcag gcgatgaagc ggcaaaaaag
     3421 aagatctgcg gcttctacct caggaacacg agatacgtga acaactggga cctcgtggac
     3481 ctttcggcgc cgaacatcgt gggcaatcac cttcaggaca agagcaggaa accgctctac
     3541 gcattcgcga aatcgcgtga tctgtggaag cgcagaatcg cgatcatggc gaccttcgcc
     3601 ttcatctagg agcatgacta tgcggacacc ctgaggatat cgggtctgct cctgaacgac
     3661 gaacacgacc tgattcacaa ggccgtggga tggatgctgc gggaggtggg caagcggtct
     3721 ctctcggccg aagaaacatt tttaaagaaa cactataaaa ccatgtcgcg gaccatgctg
     3781 cggtacgcga ttgaacggtt cccggacaag aagagaagaa agtacctgaa cggggaaatc
     3841 tgatactgac gcaaatcagt attgtcattc ccgcaaa
//
LOCUS       DASSLD010000299         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_693368,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000299 DASSLD010000000
VERSION     DASSLD010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693368"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VFK23_05400"
     CDS             complement(<1..1064)
                     /locus_tag="VFK23_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET7318555.1"
                     /translation="MFNYQPSIRQKITLGYYAIVAMIIGLTVFTFFELRFIERKIIFG
                     EAISEFFDTTLEIRRFEKNFFLYNERSDYDENGKYVARAQDILDRNLHGFNALASSQQ
                     VSQLQVGLKNYQELMEQYAGTDEHFDARRGILAVRIRTTGKDIITLAEEISKTERKSL
                     QQLLNSSQRVLVISIIILSLAVIAIGQILSRMVVKPLKSLEKSMGVIAEGEFERVAID
                     SKDREIVSLTRAFNKMLKELELHERHLIQSEKLASLGTLLSGVAHELNNPLSNISSSN
                     QILMEELSEVPGSGVRGQEKDNPAAGNAAGLAPAPGPRTPDPAFVMEMLTQINDQTDR
                     ARNIVRSLLDFSRDREFKKES"
     gene            complement(1195..>2754)
                     /gene="lon"
                     /locus_tag="VFK23_05405"
     CDS             complement(1195..>2754)
                     /gene="lon"
                     /locus_tag="VFK23_05405"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET7318556.1"
                     /translation="FDVVLTDLRMGGIDGMDLLDRIKVRHPETRVIMVTGYATVPSAI
                     EALQKGAFHYIAKPFKLDEVRSAIKLALEKKTVRANTKGSVLCFAGPPGTGKTSLGRS
                     IARALGRKFTRISLGGLKDEADIRGHRRTYVGAKPGRVIEEIRRVESANPVIMLDELD
                     KIGRDFKGDPASALLEVLDPEQNRAFVDHYLDAPFDLSSVMFILTANMLDTVPAPLRD
                     RMEVIEFSGYTEEEKAHIASLFLCPKQIREKGLSPDTVSFTDEAVVKIIREYTREAGI
                     RNLERKIATVCRKIAKASVELPGGPMPVRVTADSVERYLGRCRYRADVIEEQDRVGVA
                     TGLVRTESGGDIIFVEAAKMKGTKELIITGSLGDVMRESAQAALSYIRSNAGEFGIAE
                     DFFEHHDIHIHVPSGAIQKDGPSAGITILVALASLLTRRPARREIAMTGEITLTGRIL
                     PVGGIREKVLAAKRANVRVMIAPARNRDEVEELTADVKAGIEIRLADTVKDVVKAVLI
                     SNADFGLRNAE"
BASE COUNT          489 a          820 c          859 g          586 t
ORIGIN      
        1 gattctttct tgaattcccg gtccctcgaa aaatccagga gcgaccggac gatgttgcgc
       61 gcgcggtcgg tctggtcgtt gatctgggtc agcatttcca tgacgaaagc ggggtccggg
      121 gtccgggggc cgggggcagg ggcaagaccg gcggcattgc cggctgcggg gttatccttt
      181 tcctgacccc ggacccctga ccccggcacc tcagaaagct cttccatcag tatctggttc
      241 gatgaggata tgttcgagag agggttgttc agttcatgcg cgacgccgga gagcagcgtc
      301 cccagcgagg cgagcttctc cgactggatc aggtgccgtt catggagctc gagctccttc
      361 agcatcttgt tgaaggccct ggtcagcgat acgatctccc ggtccttcga atcaatggcc
      421 acgcgctcga actctccctc ggcgatgacc cccatgctct tttccaggga cttcagcggt
      481 ttcaccacca tgcgggacag gatctggccg atggcgatta ccgccaggga aaggatgatg
      541 atggagatga ccagaacgcg ctgggagctg ttcaggagct gttgcaggct tttacgctcg
      601 gtctttgata tttcttccgc cagggtgatg atgtccttgc ccgtagtccg tatcctgacg
      661 gcgaggatgc ccctgcgggc gtcaaagtgc tcgtccgtgc cggcgtactg ttccatgagt
      721 tcctggtagt tcttgagccc gacctggagc tgggagacct gctgagacga ggcgagggca
      781 ttgaatccgt ggaggttgcg gtcgaggatg tcctgggccc gggccacgta cttgccgttt
      841 tcgtcatagt ccgaccgttc attgtacagg aaaaaattct tttcgaaccg gcggatttcg
      901 agggtcgtgt cgaaaaattc cgagatggcc tctccgaaaa tgattttccg ctcgatgaag
      961 cgtagctcga agaaggtgaa cacagtcagg ccgatgatca tcgcgacgat cgcgtagtag
     1021 ccgagggtga tcttctgccg tatgcttggc tggtagttga acataattat atttcggaat
     1081 gcggaatgcg gaatgcggaa tgaagcagga cgaaagcgaa ctctttcgcg acgacctgac
     1141 tatattccga ccgaacaaaa gtcgtttcca tggcaaacgc aaaacagcgt gttctcattc
     1201 cgcattccgc agtccgaaat ccgcatttga tatcagcacc gccttcacga cgtccttcac
     1261 ggtgtcggcg aggcggattt cgatgccggc cttcacatcg gcagtcagtt cttcgacctc
     1321 gtcgcggttg cgcgcgggcg caatcatgac cctgacgttc gcgcgcttgg ccgcgagcac
     1381 cttctcgcgg atgccgccca cggggagtat ccggcccgtg agggtgatct cgccggtcat
     1441 ggctatctcg cgccgggccg gccgccgggt caggagcgaa gcgagggcga ccagaatggt
     1501 gatgcccgcc gaagggccgt ctttctggat cgcgccagag ggcacatgga tgtggatgtc
     1561 atggtgctca aaaaaatctt cggcgatgcc gaactcgcct gcgttgctcc ggatatagga
     1621 cagcgccgcc tgcgccgact cgcgcatgac gtccccgagg gacccggtaa tgatcagttc
     1681 cttcgtaccc ttcattttcg cggcctcgac gaagatgatg tctccgccgc tctcggttcg
     1741 aacgaggccc gtcgccacgc cgacccggtc ctgttcttcg atgacgtccg cgcgataccg
     1801 gcatcttccg agataacgtt ccacggagtc tgcggtcacc cggaccggca tcggcccgcc
     1861 gggaagttcg acggacgcct tggcgatctt ccggcagacg gtcgcgatct ttctctccag
     1921 gttccggatg cccgcctccc gcgtgtattc ccggatgatc ttgacgaccg cttcgtccgt
     1981 aaacgagacc gtgtccggcg acaggccctt ttcccggatc tgcttcggac agaggaacag
     2041 ggaggcgatg tgcgcctttt cctcctcggt gtagcccgag aactcgatga cctccattcg
     2101 gtcgcggagc ggggcaggga ccgtgtcgag catattcgcc gtaaggatga acatgacgct
     2161 cgagaggtcg aagggggcgt ccaggtagtg gtccacgaag gcgcggttct gctccgggtc
     2221 gagcacctcg agcagggccg acgcggggtc gcccttgaag tcccgcccga tcttgtccag
     2281 ttcgtcgagc atgatgacgg gattggctga ctccacccgg cggatctcct cgatcaccct
     2341 gcccggcttc gcgcccacgt aggtgcgccg gtgtccgcgg atgtcggcct cgtccttgag
     2401 cccgcccagg ctgatgcgcg tgaacttgcg gccgagggcg cgggcaatgg accggcccag
     2461 ggaagtcttg cccgtccccg gggggccggc gaaacacagg accgagcctt tcgtgttcgc
     2521 ccggacggtc tttttctcca gggcgagctt gattgccgaa cgaacttcgt ccagtttgaa
     2581 cggcttcgcg atgtaatgga aagcgccctt ctgcagggcc tcgatggccg acgggaccgt
     2641 ggcatagccc gtcaccatga tgacgcgggt ctccggatgc cggactttga tccggtcgag
     2701 gaggtccatt ccgtctatcc cgcccattcg gagatcggtg agcaccacgt cgaa
//
LOCUS       DASSLD010000300         4448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_556114,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000300 DASSLD010000000
VERSION     DASSLD010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556114"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /gene="alaS"
                     /locus_tag="VFK23_05410"
     CDS             complement(<1..1039)
                     /gene="alaS"
                     /locus_tag="VFK23_05410"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET7318557.1"
                     /translation="MKASELRKMFLDYFAARGHKVVPSSSLVPKNDPTLLFTNAGMVQ
                     FKGVFLGEDVRDYRRAVTSQKCVRAGGKHNDLENVGHTARHHTFFEMLGNFSFGDYFK
                     KDAIAFAWEFLTDVVKLPKEQLWVTVYKDDDEAFELWQQVAGVRPDRIVRLGEKDNFW
                     AMGDTGPCGPCSEIIIDQGPEMSCGKSTCAVGCDCDRYLEIWNLVFMQYNRDETGKLT
                     PLPKPSIDTGMGLERLSSVVQKVKTNFETDLFQSIIKEIASMAGVPYHKDEQTDISYQ
                     VCADPLRAMTFLISDGVLPSNEGRGYVLRRIIRRASRYGRLIGINKPFLYKLTGAVVD
                     AMREAYPELVDG"
     gene            complement(1211..1684)
                     /locus_tag="VFK23_05415"
     CDS             complement(1211..1684)
                     /locus_tag="VFK23_05415"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HET7318558.1"
                     /translation="MNDKEFTRAKNTAYRLLTYRPRSRAEIVQKLGDKGFDGAIIETV
                     VASLERMGYINDRHFAEQWASSRVRLRGYGRRRIELELRNKGVAREAIIEAFSHVFGA
                     ETEHETARHAAEKKIRTMKSADKDTRRRRLAGFLERKGFSFDVIRDVLKDMDRPN"
     gene            complement(1807..3027)
                     /locus_tag="VFK23_05420"
     CDS             complement(1807..3027)
                     /locus_tag="VFK23_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET7318559.1"
                     /translation="MNLNELLKTTAERKASDLHLKVGTAPILRINGRLTPIETEKRTT
                     QEEALTTALSIMNNVQKARFKEKNELDMAYAVPGLGRFRVNVFQQRGSVGMVFRMVPG
                     KIANFEDLMLPPILQKIAGEQRGLILVTGTTGSGKSTTLAAMIDFINTSRTANIITIE
                     DPIEFLHRDKKSIVNQREIGSDTFSFSEALRSALRQDPDVILVGEMRDFETISTALTA
                     AETGHLVLSTLHTLDAAETVNRIITVFPPYQQKQVRMQLSSVIKGIISQRLVPRVDGN
                     GRVPAVEVMLGTLSVREAVIDDTKTRQIPSIIAAGQAHYGMQTFDQSLLVLYKNGYIT
                     YDEALMTASNPDDFILKVKGIQSTSDLTWEAEDKGKSDATPGSEPSKKPGEAREYKGK
                     STDSDFKIERFGDK"
     gene            complement(3028..4131)
                     /gene="recA"
                     /locus_tag="VFK23_05425"
     CDS             complement(3028..4131)
                     /gene="recA"
                     /locus_tag="VFK23_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET7318560.1"
                     /translation="MRMADKDKLKALELAVGQIEKQFGKGSIMRLGREEVPAGIPAIS
                     TGSLGLDIALGVGGVPRGRIIEIYGPESSGKTTLALHVIAEAQKAGGQAAFIDAEHAL
                     DITYARKLGVRTDDLLISQPDTGEQALEITETLVRSGAIDVIIVDSVAALTPRAEIEG
                     EMGDAHMGLQARLMSQALRKLTGAISKSNTTLIFINQIRMKIGVMFGNPETTTGGNAL
                     KFYASVRLDIRKVSAIKDGEDVTGGRVKVKVVKNKIAPPFRAAEFDIMFNEGISKLGE
                     IIDIGVEKNIIEKSGAWFSYAGTRIGQGRENVKQFLHEHADMASEIEQKVRQASGLPG
                     GAAASAAPAKEKETAERGAKTPKGLKSEKLVEA"
     gene            complement(4251..>4448)
                     /locus_tag="VFK23_05430"
     CDS             complement(4251..>4448)
                     /locus_tag="VFK23_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HET7318561.1"
                     /translation="DRKFSPHLTLARIKYLRPRDNWQTAIGKISDVNLGGFDADHVSL
                     MKSELKPSGAVYTELGKVELR"
BASE COUNT          731 a         1373 c         1296 g         1048 t
ORIGIN      
        1 ggccgtccac aagctcgggg taggcctcgc gcatcgcatc gacgaccgcg cccgtgagct
       61 tgtacaggaa gggcttgttg atcccgatca gcctgccgta gcggctcgcc cgccggatga
      121 tcctgcggag cacgtatccc ctgccctcgt tcgacggaag aacaccgtcg gaaatgagga
      181 acgtcatggc gcggaggggg tcggcgcaga cctgatagga gatgtcggtc tgctcgtcct
      241 tgtggtaagg cacgccggcc atggaagcga tctctttgat gattgactgg aacaggtcgg
      301 tctcgaagtt cgtctttacc ttctgcacca ccgaggaaag ccgctcaagg cccatgcccg
      361 tatcgatgga cggcttcgga agcggcgtga gcttgcccgt ctcgtcgcgg ttgtactgca
      421 tgaacacgag gttccagatc tcgaggtagc ggtcgcagtc gcaccctacg gcgcaggtcg
      481 acttgccgca ggacatctca ggcccctggt cgatgatgat ctcggagcag ggtccgcagg
      541 ggcccgtgtc gcccatagcc cagaagttgt ccttctcgcc caggcgcacg atgcggtcgg
      601 gccgtacacc cgcgacctgc tgccagagtt cgaaggcctc gtcgtcgtcc ttgtagaccg
      661 tcacccagag ctgctccttc gggagtttga cgacgtcagt caggaattcc caggcaaagg
      721 cgatggcgtc cttcttgaag taatcgccga aggaaaaatt gccgagcatc tcgaagaacg
      781 tgtgatggcg ggctgtgtgg cccacgtttt cgaggtcgtt gtgcttgccc ccggcgcgca
      841 cgcacttctg ggaggtcacg gcccgccggt agtcccggac gtcctcgccc aggaacacac
      901 ccttgaactg caccattcct gcgttcgtga aaagcagggt ggggtcgttc ttgggcacga
      961 gggacgagct cggcacgacc ttgtggcccc gcgcggcaaa atagtcgaga aacatctttc
     1021 gtagttcgct ggctttcaat tcatgctcct tcgaatgaaa attatcacaa taataacatt
     1081 attggatgtc attcccgcaa agttctgagc gggaatccat tttcataaag tcaaaatgga
     1141 tccccgatag aggctttcgg ggatgacaat ctatctggcg ttctaatttg cggatattat
     1201 tttgagccct tcaattaggc cggtccatat cttttaacac atctctgatg acatcgaacg
     1261 aaaacccttt tcgctccaga aacccggcaa ggcgtcttct gcgggtgtcc ttatcagcgg
     1321 atttcatggt cctgatcttt ttctccgccg catgcctcgc cgtttcatgc tctgtttcag
     1381 ccccgaaaac atgggaaaac gcttcgatta tggcttctct ggccacccct ttgttccgga
     1441 gttccagttc tatcctgcgt cttccgtatc cccggagcct tacccggctg gaagcccatt
     1501 gctccgcgaa atgccggtca ttgatatacc ccatccgctc cagggaggca acgaccgttt
     1561 cgatgatcgc cccgtcgaag cccttgtcgc cgagtttttg cacgatctcc gcccggctcc
     1621 gcggccggta ggttaagagc cgataagcgg tattcttggc gcgggtgaat tccttatcat
     1681 tcaattaact gcgccgatcc cttctttccc cgcttgacga gagacgatcg ttcgctgcgc
     1741 tcactaggac gatggacgca atgtcttcat ttcgtccctc gtccatcgtc tctcgtccat
     1801 cgctcgctac ttgtccccga accgctctat cttgaagtcg ctgtccgtcg acttgccctt
     1861 gtactcccgg gcctcgcccg gcttcttcga cggctcgctc ccgggcgtcg catcggattt
     1921 tcccttgtcc tcggcctccc acgtcagatc gctggtggac tggatgccct tgaccttgag
     1981 gataaaatca tcggggttgc tcgccgtcat cagggcttcg tcgtaggtga tgtagccgtt
     2041 tttgtagagc acgaggagcg actggtcgaa ggtctgcatg ccgtaatggg cctggccggc
     2101 cgcgatgatc gacgggatct ggcgcgtctt ggtatcgtcg atgacggcct cgcgcacgga
     2161 aagcgtcccg agcatcacct ccacggcggg tacgcggccg ttaccgtcca cccgggggac
     2221 aaggcgctgg gagattatcc ccttgatgac ggaagagagc tgcatgcgga cctgcttctg
     2281 ctgatagggc ggaaacaccg taatgatgcg gttgaccgtc tccgccgcgt ccagcgtatg
     2341 gagggtcgac aggacgaggt ggccggtctc cgccgcggta agcgccgtgg aaatggtctc
     2401 gaagtcgcgc atttccccca ccaggatcac gtccgggtcc tgccggaggg cgctgcggag
     2461 tgcttcgctg aaactgaagg tatcggagcc gatctcgcgc tggttcacga tgcttttctt
     2521 gtcccggtgt aggaattcga tggggtcctc gatcgtgatg atgttggccg tgcgcgacgt
     2581 gttgatgaag tcgatcattg ccgcaagggt cgtcgacttg ccgcttcccg tcgttcccgt
     2641 cacgaggatg aggcctcgct gctcgcccgc aattttctgg aggatcgggg gcagcatcag
     2701 gtcctcgaaa ttcgcgatct tgcccggcac catgcggaaa accatgccca cgctgccgcg
     2761 ctgctggaac acgttcacgc ggaagcggcc gagccccggc acggcgtagg ccatgtcaag
     2821 ctcgtttttc tccttgaacc ttgctttctg cacattgttc atgatgctga gcgcggtcgt
     2881 gagcgcctcc tcctgcgtcg tgcgcttctc cgtctcgatc ggagtaagac ggccgttgat
     2941 ccgcaggatg ggagcggtcc ccactttcag gtgcaggtcc gaggcctttc tttccgcggt
     3001 cgtcttgagc aattcgttga gattcattta cgcctccaca agtttttccg acttcagtcc
     3061 cttgggggtc tttgctcccc gttcggcggt ttccttttcc ttcgccgggg cggcggatgc
     3121 ggcggctccg ccgggaagcc cgctcgcctg ccggaccttc tgttcgatct cggaagccat
     3181 gtccgcgtgc tcgtgcagga actgcttcac gttctcgcgg ccctgcccga tcctcgtccc
     3241 cgcataggag aaccatgcgc cgctcttttc aataatgttc ttctccacgc cgatgtcgat
     3301 gatctcgccg agcttggaga tcccctcgtt gaacatgata tcgaattccg ccgcgcggaa
     3361 agggggagcg atcttgtttt tcaccacctt gaccttcacg cggccgccgg tcacgtcttc
     3421 gccgtccttg atggccgaga ccttgcggat gtcgaggcgg acggacgcgt agaacttgag
     3481 cgcgttgccg cccgtggtcg tctcggggtt cccgaacatg accccgatct tcatgcggat
     3541 ctggttgatg aagatgagcg tcgtgttgga tttgctgatg gcgccggtga gcttccggag
     3601 ggcctggctc atgagcctgg cctggagccc catgtgggcg tcgcccatct ccccctcgat
     3661 ctcggcccgg ggcgtgaggg ccgcgacgga gtccacgatg atcacgtcga tggccccgct
     3721 ccggaccagc gtttcagtga tttcgagcgc ctgttccccc gtatcgggct gggagatcag
     3781 gaggtcgtcg gtcctgaccc cgagcttgcg cgcgtaggtg atgtcgaggg cgtgctcggc
     3841 gtcgatgaag gcggcctggc cgcctgcctt ttgggcttcg gcgatgacgt gcagggccag
     3901 ggtcgtcttg cccgacgact cggggccgta gatctcgatg atcctcccgc gcggaacgcc
     3961 gcccacgccc agggcgatgt ccagcccgag cgagcccgtt gatatcgcgg gaatgcccgc
     4021 ggggacctct tccctcccca gccgcatgat ggagcccttg ccgaactgct tttcgatctg
     4081 gcccaccgcc agttccagcg cctttaactt gtccttgtcc gccattctca ttctcctttc
     4141 gccgtcgcgt ggctgtccgt ttctatgaat ctgtgtccgc atagcgcatg gtgcatggtg
     4201 tctaaaatct tttcgctctg cgccatgctc cgtgctccat gcgttttctc ttacctcagt
     4261 tccaccttcc ccaactcagt ataaacggcc cctgacggct ttagttcgct cttcatcagg
     4321 ctcacatggt ccgcatcgaa gccgccgagg tttacgtcgc ttattttccc gatcgccgtc
     4381 tgccagttgt cccgcggccg cagatacttg atccgggcca gggtaaggtg tggggaaaac
     4441 ttccggtc
//
LOCUS       DASSLD010000301         2924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_986806,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000301 DASSLD010000000
VERSION     DASSLD010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986806"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VFK23_05435"
     CDS             <1..1076
                     /locus_tag="VFK23_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7318562.1"
                     /translation="QLIVKLFGEDINVLGKKSAEIAKVLSTISGGTDLSAEKASGQPY
                     LTINIDRSKIARYGLNISDVQKVIEIAVAGKAASQLYEENRSFPVTVRLPEEKRNSLE
                     AVENLLITTKTGANLPLGQLAEVKLIEGPLQISRQDGERRIGIEMNITGRDIGSFVAE
                     AKQKIKEQVKLPAGYYLTWGGQFENQQRAMHKLMIIGPVAVGMILLLLYVSFRSVRLA
                     LLVISNLPFALIGGVFALFLTGQYLSVPASVGFIVLFGVAVLNGLVLVSRISQLREEG
                     LELQEAIRKGSMDRLRPVLMTASIAIFTLLPMLVAGGTGSEIQRPLATVVVGGLFTST
                     LLTLLIIPSVYGWFEKRTAEAEG"
     gene            1178..1783
                     /locus_tag="VFK23_05440"
     CDS             1178..1783
                     /locus_tag="VFK23_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318563.1"
                     /translation="MNKYVYSLCVALLFAALSGCGAAKMIAAKTQSERSDIFTEAAGS
                     EPAPAGYADVIITADIKTHLEGCYKGESASSAHGKEAYPYLFNIDGQAVLWKAEGKKH
                     ELPKYDPDGKTSRNPEAGEGMKYVLKKKIRLQAGAHRLFFGLPEDDYYREIPILVKEG
                     ETLPLDFKPVYRYKTLPTRTQTFMRGVSHYDIFVNNVLVTQ"
     gene            1791..2621
                     /locus_tag="VFK23_05445"
     CDS             1791..2621
                     /locus_tag="VFK23_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET7318564.1"
                     /translation="MQRKEMAVRNCVIVLLVAASFAASVLTAGAEEAGKIQDNSFLLE
                     VAYNQEDGVVQHIQTWMYIKKTKSWLYTFTQEWPVPKQTHQLSYSIPVAHVVEGQPET
                     GLGDAALNYRYQLLMAGPYALAPRISVLLPTGDYKKGMGSGATGYQVNIPFSMELSDE
                     WVTHWNAGMTFTPGAKEPGGATADTTGYNLGASVIWLTSETFNLMTEIAFNRFQTVQP
                     DGGRAWEETFFVNPGMRFAMNYRSGLQVVPGLAFPVGIGTSHGEYGVFLYLSFEHPLF
                     "
     gene            complement(2797..>2924)
                     /locus_tag="VFK23_05450"
     CDS             complement(2797..>2924)
                     /locus_tag="VFK23_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7318565.1"
                     /translation="AAFQVGYESPSQFSREYSRLFGAPPLRDITNLRQTATTDVG"
BASE COUNT          666 a          779 c          878 g          601 t
ORIGIN      
        1 cgcaactgat cgtgaaactc ttcggcgagg atatcaatgt ccttggcaaa aaatccgctg
       61 agatcgcaaa agtactctcc acgatcagtg ggggcaccga cctgagtgcg gaaaaggcgt
      121 ccggacagcc ttatctgacg atcaatatcg accgctcgaa gatcgcccgc tacggcctga
      181 acatcagcga cgttcagaag gtgatcgaga ttgccgtcgc gggcaaggcg gcatcacagc
      241 tgtacgagga aaaccgcagt tttcccgtca cggtgcgcct gccggaagag aaacggaact
      301 cgctcgaagc ggttgagaac ctgctgatca ccacgaaaac aggggccaac ttgccgctcg
      361 gacagctggc ggaagtgaaa ctgatcgagg gaccgctcca gatcagccgc caggacgggg
      421 agcgccggat cggcatcgaa atgaacatca ccggcaggga catcggcagt ttcgtcgccg
      481 aggccaagca gaagatcaaa gaacaggtca agctgccggc aggctattat ctgacctggg
      541 gaggacagtt cgagaaccag cagcgggcga tgcataagct gatgatcatc ggtcctgtcg
      601 ccgtgggcat gatcctgctt ctcctctatg tatccttccg gtcggtccgg cttgcgctcc
      661 tggtcatttc caacctgccc ttcgccctga tcggcggcgt cttcgccctc ttcctcaccg
      721 ggcagtatct gtccgtgccg gcgtcggtcg ggttcatcgt gcttttcggc gttgcggtgc
      781 tgaacgggct ggtgctggta tcgcggatct cgcagttgcg cgaggaaggg cttgagctgc
      841 aggaggcgat caggaaagga agcatggacc gtttgcggcc ggtattgatg accgcgtcga
      901 tcgcgatctt cactctgttg ccgatgctcg tggcaggcgg gaccggttcg gaaatacaaa
      961 ggccgcttgc aaccgtcgtc gtgggagggt tgtttacctc gacgctcctg acgctcctga
     1021 tcattccttc ggtgtatgga tggtttgaaa agcggactgc cgaggcggaa gggtaaatgc
     1081 cgtaaaggag tctcgacggt ctattcgata agaggccagg ggaggcgctc cgaaggtgag
     1141 aaaagcgata agcgggacgc agaataggag aaggaaaatg aataaatatg tctactctct
     1201 gtgtgttgct ctgttgtttg cggcgctttc aggatgcggc gcggcgaaga tgatcgccgc
     1261 gaagacgcag agcgagagga gcgatatttt caccgaagct gccggttcgg agcctgcacc
     1321 ggccggatac gcggacgtca tcattaccgc cgacatcaag acccacctgg aagggtgcta
     1381 taagggcgaa tccgcgtcgt ctgcgcacgg caaggaagcc tatccctatc tgttcaacat
     1441 agacggccag gccgttctct ggaaggccga gggaaagaag catgagcttc cgaagtatga
     1501 tcccgatggg aaaacgagcc ggaacccgga ggcgggcgaa ggcatgaagt atgtgctgaa
     1561 gaagaagatc aggctccagg ctggggctca ccggctgttc ttcggacttc ccgaggacga
     1621 ctattaccgg gaaatcccga tccttgtcaa ggaaggcgag acgctgccgc ttgattttaa
     1681 acctgtatat agatacaaaa cgctgcctac ccgcacccag acattcatgc ggggcgtcag
     1741 tcattatgat atattcgtaa acaatgtcct cgtgacgcag tgaaccgggg atgcagagaa
     1801 aggaaatggc cgtgaggaac tgcgtgatcg tattgctcgt ggctgcgagc ttcgccgcat
     1861 ctgtcctgac ggccggcgcc gaagaggccg gaaagatcca ggacaactcg ttcctgctgg
     1921 aggtggcata caaccaggag gacggggttg tacagcatat tcagacctgg atgtacataa
     1981 agaaaacaaa gtcgtggctg tacacgttca cccaggaatg gccggtgccg aagcagacac
     2041 atcagctgtc ctacagcata cctgtcgcac acgttgtcga aggtcagccg gagacaggcc
     2101 tcggtgacgc ggccctcaat taccggtatc aattgctcat ggccggtccc tatgcgctcg
     2161 caccgagaat ctcagtcctc ctgccaacgg gtgattacaa gaagggtatg gggagcggag
     2221 caacggggta tcaggtcaat attccgttca gcatggagct gtcagatgaa tgggtgacgc
     2281 attggaacgc agggatgacg tttactcccg gcgccaaaga gccaggcggc gcaacagccg
     2341 atacaacagg atataacctc ggcgcgagcg ttatctggct cacatcggaa acgttcaacc
     2401 ttatgacgga gattgccttc aacaggtttc agaccgtcca accggacggc ggccgggcat
     2461 gggaggaaac cttttttgtc aatcccggca tgcgctttgc gatgaattac cgatccggcc
     2521 tgcaggtagt accgggcctg gcgtttcccg tcggcatagg aacatcgcac ggtgaatacg
     2581 gcgtattcct gtatctctct tttgaacacc cgctgtttta gcattcgtgt gctcctccga
     2641 agcatgagga aaacctttcc ctcaaattcc ctttggttcg atcccccctt atcaggtaat
     2701 aaaaaaggat tggcatcttg ccaacccttt ttgttgtctt ggtggaactg agggggatcg
     2761 aacccccggc agtctcccat cgcatgaatt ttctgactac cccacatctg ttgtagctgt
     2821 ctgccgcaag ttcgtgatgt ctcgcagcgg tggagcgccg aacaggcggc tgtactcgcg
     2881 gctgaattgg gaggggctct catagccgac ctgaaacgcc gcgg
//
LOCUS       DASSLD010000302         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_156383,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000302 DASSLD010000000
VERSION     DASSLD010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156383"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VFK23_05455"
     CDS             complement(<1..410)
                     /locus_tag="VFK23_05455"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilB"
                     /protein_id="HET7318566.1"
                     /translation="MTSFRRKRLGDILVVKGAVTQQQITAVLAKPEARHMRTGELLLA
                     EGLITEEILAQTLAEQRGLRYADLADFRINPKFFETIPVEFMQRYQFVPLEDGGDILH
                     VAMTDPNNLPAIDELEMILNRQLDISVCTFASVQA"
     gene            complement(407..1594)
                     /locus_tag="VFK23_05460"
     CDS             complement(407..1594)
                     /locus_tag="VFK23_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7318567.1"
                     /translation="MPLFVYKAVREDGTSFTEEAMAGDAAELRRDLEGRGYLVLSLVK
                     KKTVIRGRASTKDFLLFNQEFSTLVKAGLPILQSLEILHKRTEKPGFRSALESIIHDI
                     KGGTALSDAMARHPAYFSPLYTSTVRAGEKSGALVDVLKRFIAYQKKMIAVMSKLKLS
                     LAYPAFLVVALAAVLMLFFLYVIPNFTKMYSDQSGKLPALTQFLISFTNMLTEFAPFI
                     FAGFIALGIALSLWRRTAAGRTAIDGLKLRIPLLRALITQYLLAQITRTLATVLRGGI
                     PLVQALETTTGVINNKVMSAKLSEVKTLVTEGVSLALAFERTRLAPDMTVRMIEVGES
                     SGDLPEMLEDIADFYDQEVENRLIAITTMIEPVLMLVMGLVMAVIVVALYLPIFEMGS
                     RLK"
     gene            complement(1706..>2317)
                     /locus_tag="VFK23_05465"
     CDS             complement(1706..>2317)
                     /locus_tag="VFK23_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HET7318568.1"
                     /translation="IAARYAQKRYGLERILIVDWDLHHGNGTQRAFYDDPAVLFFSSH
                     QYPYYPGSGGVEEVGSGKGEGYTVNAPFPVGFGDAGYIHVYSNLLKPIALEYRPELVL
                     VSAGFDPYVRDPLGGMEVTGEGFGALAAIVRAIADESCPGRLLLSLEGGYNPEGLRSG
                     VRAVIDSLLGRSAPPRGKTASRAEEQAARRIIDVHKQYWRSLR"
BASE COUNT          451 a          666 c          738 g          462 t
ORIGIN      
        1 gcctgaacgg acgcaaaggt gcagacgctg atgtcgagct ggcggttcag gatcatctcc
       61 agttcgtcga tggcaggaag attgttggga tccgtcatgg cgacatgcag gatgtcgccc
      121 ccgtcttcca gcgggacgaa ctgatagcgc tgcatgaact cgaccgggat ggtttcgaaa
      181 aatttcgggt tgatccggaa atccgcgaga tcggcgtagc gcagaccccg ctgctccgcc
      241 agggtctggg cgaggatctc ctcggttatc aggccttcgg cgaggaggag ctcgccggtg
      301 cgcatgtggc gggcctcggg cttcgcaagg acggctgtga tctgctgctg ggtcactgcc
      361 ccttttacca ccaggatatc gcccagccgt tttcttctga atgacgtcat tttaacctcg
      421 atcccatttc gaatatgggc aggtagaggg ctactacgat caccgccatg acgagcccca
      481 tgacgagcat gagcacgggc tcgatcatgg tcgtaatcgc gatcagcctg ttctccacct
      541 cctggtcata aaagtcggcg atgtcttcga gcatctcggg aaggtcgccg gacgattccc
      601 cgacctcgat catgcgcacg gtcatgtccg gcgcgagccg ggttcgttcg aacgcgagcg
      661 ccaggctcac gccttcggtc acgagcgttt tgacctcaga cagcttcgcc gacatgacct
      721 tgttgttgat cacgcctgtc gtggtttcga gcgcctggac cagcgggatg cccccccgga
      781 gcacggtcgc aagcgttctc gtgatctggg ccaggaggta ctgcgtaatg agggcccgca
      841 ggagcgggat cctcagcttc agaccgtcga tcgcggtgcg gccggccgcg gtcctcctcc
      901 acagcgagag tgcgatgccc agcgcgatga accctgcgaa gatgaacggc gcgaattcgg
      961 tcagcatgtt cgtgaaggat atgaggaact gggtcagcgc gggaagtttc cccgactggt
     1021 cgctgtacat cttggtgaag ttcgggatga cgtagagaaa aaagagcatg agcaccgcgg
     1081 ccagggcgac gacaaggaag gccggatagg cgagcgacag cttcagtttg ctcatcacgg
     1141 ctatcatctt cttctggtac gcgatgaaac gcttgagcac gtccacgagc gcgccgcttt
     1201 tctcgccggc ccgtaccgtg gacgtgtaga gcggcgagaa gtaggccgga tgccttgcca
     1261 tggcgtcgga aagcgccgtt ccccccttga tgtcatggat gatgctctcg agcgcgctcc
     1321 ggaatccggg tttttccgtc cgtttgtgca ggatttcgag ggactggagg atggggagcc
     1381 cggccttgac gagcgtggaa aactcctggt tgaacagaag gaagtccttc gtgctggcgc
     1441 ggccgcgtat gaccgttttt ttcttcacga ggctgaggac aagataacct ctcccctcga
     1501 ggtcccttct gagctccgca gcatcccccg ccatcgcctc ctcggtgaac gacgtgccgt
     1561 cttcgcgcac ggctttatag acaaacagag gcataaaatg ataataccag atagactttg
     1621 tataagtcaa taagtgctgc gcgggagagc ggtgaaatgg gcgggaaatg ggctcaacgg
     1681 cctgccgggg ccgggacgga aaggtctacc gcaggctcct ccagtattgc ttatgcacat
     1741 cgatgatccg ccgggccgcc tgttcttcgg cgcgcgacgc ggtttttccc cgaggggggg
     1801 cggaacggcc gaggagggaa tcgatcacgg cccggacgcc gctccgcagg ccttcggggt
     1861 tgtaccctcc ttcgaggctc agcaggagtc tgccggggca gctttcgtcc gcgatcgccc
     1921 tgacgatggc cgcaagggcg ccgaatccct cgccggtcac ctccatgccg ccgagggggt
     1981 cccggacata gggatcgaaa cccgcggaaa cgagaacgag ctcgggacgg tattcaaggg
     2041 caatcggttt caacaggttg gaatagacat ggatataccc ggcgtcgccg aacccgacgg
     2101 ggaagggggc gttgacagtg tagccttctc ccttgccgct tcccacttct tcgacgccgc
     2161 cggagccggg atagtaagga tactggtgcg aagaaaagaa gagaactgcg ggatcgtcat
     2221 aaaaggcccg ctgtgtgccg ttaccgtggt gcaggtccca atcgacaatc agaatccgtt
     2281 cgagcccgta ccgtttctgt gcgtaccggg cggcgat
//
LOCUS       DASSLD010000303         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_144949,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000303 DASSLD010000000
VERSION     DASSLD010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144949"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFK23_05470"
     CDS             complement(<1..516)
                     /locus_tag="VFK23_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET7318569.1"
                     /translation="MKKDALYRSTVVLPAVVLAMALLGACGNSGKKTTSPATAQNLSS
                     AAAVGMNTCTTCHTAVTADWLASRHANLDPGSLDSPGNPTLAQISGCSVNCHDPGGDS
                     GRLVAGFTGNVRRPVVGCEDCHGPGSLHAQGGGTGPIGFAATDAGAIGSTQASAQFRT
                     CTRCHELLDTAG"
     gene            complement(586..1275)
                     /locus_tag="VFK23_05475"
     CDS             complement(586..1275)
                     /locus_tag="VFK23_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET7318570.1"
                     /translation="MDILAARKKAAEKAKMQQEPEPAPAAEPGPVEAERAEVSPEPAA
                     PPAPAPVPQSTEQTALPPAPAVAAPSAPESPGQGEAAGAGEVVKETTQELELLSFQLG
                     AEEYAVMVDDVREVLKLRELTDVPNTPEYILGVISLRGTMLTVIDLGRRLGLAPGVRD
                     ERARIIVASPDEEDVGLLVDRVTGVLKIMDEAIKPPPENVEQGAEFLRGIVRKDDRLV
                     ILLDLAKAVGT"
     gene            complement(1431..2225)
                     /locus_tag="VFK23_05480"
     CDS             complement(1431..2225)
                     /locus_tag="VFK23_05480"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET7318571.1"
                     /translation="MYEEFYGLREKPFNRTPDPRFLYQSPKHAEALARLQLAVEEQDI
                     VLLTGEIGAGKTTLSRAFIDGLDAGFHPVLIINPRLSPSQLLRTIALRLGMEETPRHR
                     HELLEAMNAKLYEIYEAGRRTAIIIDEAQLIPGKATFEELRLLTNFQLDDRNLLAIVL
                     IGQTELRDRLNRKPYRALRQRIGMQYHLGPLDREETGAYVRHRLEVAGRSTPLFDERA
                     LDLLYEHSGGIPRRINIIAGNALIEGFGRSALTIGPEIIESAVEDQ"
     gene            complement(2363..>2599)
                     /locus_tag="VFK23_05485"
     CDS             complement(2363..>2599)
                     /locus_tag="VFK23_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET7318572.1"
                     /translation="ELDAFYVILVGLAERRVGIIVDNLRDQQEIVIKPLGKRLADVPG
                     IAGATELGDRRGVVLVLDVESLIEGAMKKTAIKA"
BASE COUNT          433 a          842 c          783 g          541 t
ORIGIN      
        1 tcccgcagtg tccagcagtt catgacagcg ggtacaggtc cggaattgcg ccgatgcctg
       61 ggttgatccg atcgcgcccg catccgtggc cgcaaagccg atcggccccg tcccgcctcc
      121 ctgcgcgtgc aggcttcccg gaccgtggca gtcctcgcac cccaccacgg gacgccggac
      181 gttcccggtg aagccggcaa cgagtctccc gctgtcgccg ccgggatcat ggcagttcac
      241 cgaacagccg gagatctggg cgagcgtggg attgccgggg ctgtccaggc tgccgggatc
      301 aaggttcgcg tgcctgcttg ccagccagtc tgcagtgacg gcggtgtggc aggtcgtgca
      361 cgtgttcatg cccaccgctg cggccgacga cagattctga gcggttgccg gcgatgtggt
      421 ctttttgccg gaattgccgc aggctccgag cagggccatc gccaggacca ctgccggcag
      481 caccacggta ctgcggtaca acgcgtcctt tttcatactc tcttcctccc ccctcaatcg
      541 attgcgtata cgaattacgg atttcggaat gaaaaaaatc ggcaattatg tcccgactgc
      601 ctttgcaagg tccaggagaa tgaccagtct gtcgtccttg cggacgatac cgcgaagaaa
      661 ctccgccccc tgttcgacgt tttccggagg cggcttgatt gcttcgtcca tgatcttgag
      721 cacgccggtc acgcggtcga ccaggagccc cacgtcctcc tcatcggggc tcgcgacgat
      781 gatgcgcgcc cgttcgtctc gaacgcccgg tgcaaggcca agcctcctgc cgaggtcgat
      841 gaccgtgagc atcgtgccgc ggagcgatat cacgccgaga atgtactcgg gcgtattggg
      901 gacatctgtc agctcgcgga gcttcagcac ctcccgcacg tcgtccacca tgacggcgta
      961 ctcctccgcg ccaagctgga acgagagcaa ttccaactcc tgcgtcgtct ctttcacgac
     1021 ctcgcccgct cctgccgctt caccctgccc cggactctcc ggggccgagg gggcggcgac
     1081 tgcgggggct ggcggcagcg cggtctgctc ggtcgactgc gggaccggcg cgggtgccgg
     1141 gggggcggcg ggctcgggcg agacttccgc ccgctcagct tcgacaggcc cgggttccgc
     1201 agcaggagcg ggctctggct cctgctgcat cttggctttt tccgccgctt tttttcgcgc
     1261 tgcaaggata tccattagtc gctacgctcc agttcagtat caacgaggga cgacggacga
     1321 ccgctctcgc actgcatgag ggacgaagga cgatcgttcg ctgcgctcgc taggacgatg
     1381 gacgcaaaat ctttttcgtc cttcatccct cgtccatcgt ctctcgtccg tcattgatcc
     1441 tccaccgcgc tctcgatgat ctccggcccg atcgtcaggg ctgacctccc gaatccttca
     1501 atgagagcgt tgccggcaat gatgttgatc cggcggggaa tgccgcccga gtgttcatac
     1561 aggaggtcca gcgcccgctc gtcgaaaagc ggcgtgcttc ttccggccac ttcgagacga
     1621 tgccgtacgt atgcccccgt ctcttcgcgg tccagcggcc ccaggtgata ctgcatcccg
     1681 atgcgctgac gcagggcccg gtacggcttg cggttcaggc ggtcgcgcag ctccgtctgg
     1741 ccgatgagga cgatcgcgag cagattgcgg tcgtcgagct gaaaattggt caggagccgc
     1801 agctcctcga acgtcgcctt gcccggtata agctgggctt cgtcgatgat gatcgccgtc
     1861 cgcctgcctg cctcgtagat ctcgtagagc ttggcgttca tcgcctctaa gagctcatgg
     1921 cggtgccgcg gggtctcttc catgccgagc cggagggcaa tggtgcgcag aagctgagac
     1981 ggcgagagcc gcggattgat gatcagcacg gggtgaaagc ccgcatccag tccgtcgatg
     2041 aacgcccggg acagggtggt cttgcccgcc ccgatctcgc ccgtcagaag aacgatgtcc
     2101 tgctcctcga cggcgagctg gagccgggcg agcgcctcgg cgtgcttcgg gctctggtac
     2161 aggaaccgcg gatcgggcgt cctgttaaac ggcttttccc tcagtccgta aaattcttcg
     2221 tacatccgtc aggccaagct ccaaatctca agcaccaaat ttcaaataag ccccaagcac
     2281 taaattccag acttttcggt cattgtcttt tggaacttgg aatttatttg ggatttggaa
     2341 tttgtcattt ggaatttttc ccctatgcct ttatcgcagt tttcttcatc gccccctcga
     2401 tgagcgactc gacgtcgaga acgaggacca cgccccttcg gtcgcccagt tcggtggccc
     2461 cggcgatgcc cgggacgtcc gccagccgtt ttccgagggg tttgatgacg atctcctgct
     2521 ggtcccggag gttgtctacg atgatcccca cccgccgctc ggcaaggccg acgaggatga
     2581 catagaacgc gtcgagctc
//
LOCUS       DASSLD010000304         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_15278,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000304 DASSLD010000000
VERSION     DASSLD010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15278"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..473)
                     /locus_tag="VFK23_05490"
     CDS             complement(186..473)
                     /locus_tag="VFK23_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318573.1"
                     /translation="MAMADTDFQGEQLRRRCAISGWLSAGGFAFSFLYPQFDQSMDSP
                     FILGIYTLVVGVVGCVTYKIRHSLHKVAQRRKMRKIRDANSELIFLCGGIS"
     gene            965..1252
                     /locus_tag="VFK23_05495"
     CDS             965..1252
                     /locus_tag="VFK23_05495"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HET7318574.1"
                     /translation="MTTFIMTGKYSVESIKQISGERTTKGVQIVQQCGGKFVAAYATM
                     GETDVLAIVEFPGVSEAIKASVALNKALGISFSTVPALYLEDFDKLVGGKG"
     gene            1442..2209
                     /gene="hisF"
                     /locus_tag="VFK23_05500"
     CDS             1442..2209
                     /gene="hisF"
                     /locus_tag="VFK23_05500"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HET7318575.1"
                     /translation="MEPVKIMPCLDMKEGRVVKGINFVNLRDAGDPVKNAEFYQAEGA
                     DELAMLDIAATLENRKTRLEWVKSVSAVIDIPLTVGGGIASLQDIEMTLKAGAKKVSM
                     NSAAVKDPELVMRAAGGFGKETVCVAIDGRRNSSLPSGFEVVVAGGTKGTGLDCGEWA
                     RRCQELGAGVILPTSMDGDGTLAGYDLAFTRLVADAVDLPVIASGGAGTLEHFYQAVT
                     EGGADVLLAASVFHFRTLSIGQVKKYLKGRGIPVLGV"
     gene            complement(2465..>2847)
                     /locus_tag="VFK23_05505"
     CDS             complement(2465..>2847)
                     /locus_tag="VFK23_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318576.1"
                     /translation="IASSSRRGGWRILGFGKHPEIAATVQEKLRSLGFQATNFVLTND
                     EAGDARLIAELKRAEYDGVAIGGYINGQDAVNFPATEETTVWFNRVLNIIHANAPGSK
                     IILVRGPEDVVPAIERVLGKNPPS"
BASE COUNT          644 a          786 c          821 g          596 t
ORIGIN      
        1 ttaaattaaa atggtcccaa ttcccgctgc aatcatggag gggtttccct cttcaacacc
       61 ctccccgcga atcccctttt ctcggctaaa taaaaaagga gttacggcat tttccataac
      121 tccttaattt tatggtggcc cctcccggaa ttgaactggg gggcaccatc ttttcttcgg
      181 cccgtttagc tgattccccc gcagaggaat atcagttcgg aatttgcatc cctgattttc
      241 cgcatcttcc gtcgctgggc gaccttgtga agggaatgac ggatcttgta cgtgacgcac
      301 ccgacaaccc cgaccaccag ggtataaatc cccaatatga acggactgtc catcgactgg
      361 tcgaactggg ggtacaggaa cgagaaggca aagccgccgg cggaaagcca cccgcttatg
      421 gcgcaccgcc tcctcaattg ttccccctgg aaatcggtat ccgccatggc cattgggagt
      481 tctcccattt gatcctttct gccacctcga acaacatatt attcaaacag tatgccgacg
      541 caggaatgcc ggaaagtaat cggatataaa tgattttatt cttacaaacc cacgccagtg
      601 acggtttatt gtcccttgtt ggaaataatt ttccgcttct ggctgaacgg acggaaatcc
      661 cgtcatcaac ttcaactaaa ccgggaaaat ttaattaaaa atggcccaaa tctacttggc
      721 catcatgtga gcccacggcc tgtttttacg aaaaatgggg aaaccccaca tcaatccgga
      781 ttcccattga gtatttagat ggtgccgagc gggaattcga actccaacca ggtccccacc
      841 acccccatga tcgcaagaaa tataaggagt atcgagaaat accctgggtg atatgaaatg
      901 atatgggagt atgcgttcca tcaaccgttc tctagggcca aaacaatgaa aggaggaaag
      961 tgcaatgaca acatttatca tgacggggaa atattctgtt gagtcgataa agcagataag
     1021 cggcgagcgc accacgaaag gggtccagat agtccagcag tgcggtggaa agttcgtggc
     1081 tgcctacgcc acgatggggg agactgacgt gttggcgatc gtcgaatttc ccggcgtaag
     1141 cgaagcgatt aaagcttctg tcgccttgaa taaggcactc gggatttcct tctccaccgt
     1201 gcctgcgctc tatcttgagg acttcgacaa actggtaggt ggaaaagggt agataagatt
     1261 gtatctaagg ggggaaactc gaaccccagg agtttccccc tccccctgag accccaattt
     1321 caccccgaaa atggacgttg tttgcacggg gtttgcagtc cgttcggttt tcttccccga
     1381 cagaggtaga atgccgacca aggcaccatc tcgatcggga gaaaacccaa ggggggatag
     1441 catggaacct gtgaagatca tgccctgcct cgacatgaag gagggacggg tcgtcaaagg
     1501 catcaatttc gtcaacctgc gcgacgccgg ggacccggta aagaacgcgg agttctacca
     1561 ggcggaggga gcggacgaac tggccatgct ggacatcgcc gccacgttgg agaaccgcaa
     1621 aacgcgtctg gaatgggtca agtcggtctc cgcagtgatc gacatccccc tcacggtggg
     1681 aggggggatc gccagcctgc aggacatcga gatgaccctg aaggcgggcg cgaaaaaggt
     1741 ttcgatgaac agcgcggcgg tgaaagaccc ggagcttgtg atgcgggccg ccggaggttt
     1801 cgggaaggaa accgtttgcg ttgccatcga cgggcgccgg aactcctccc ttccgtccgg
     1861 gttcgaggtg gtggtggcag gcgggaccaa agggacgggg ctggattgcg gggaatgggc
     1921 ccgacgttgc caagaactgg gggcgggggt gattctgcct acaagcatgg acggggacgg
     1981 tacgttggcg gggtacgacc tcgccttcac ccgcctggta gccgacgccg tggacctgcc
     2041 ggtgatcgct tccggcgggg cgggaactct ggagcacttc taccaggcgg tgacggaagg
     2101 tggcgccgac gtcctcctgg ccgcctcggt gttccacttc cggacactct ccatcggcca
     2161 ggttaagaag tacctgaagg gaagagggat tcccgtgctg ggagtgtaag ggggtcagat
     2221 ctcccgccga cccgctgatg cagaagtcct gccatgacgt agaggaccgc cccaatccct
     2281 atggcccatc agaagcggag cgatagggcg ccgttcgccg cgagcagttg tatgcacggg
     2341 gtgtggggtc cgtgcggatt cccctatggg actgataagg gcctccagac tactcaagag
     2401 gcctttctgt tctcatcatg gtgccgaagg ggagtcgaac cccactggtt ttcctgcctc
     2461 cccctcaaga tggcgggttc ttaccgagca cccgttcgat cgccggcaca acgtcctccg
     2521 gaccgcgcac caggatgatc ttcgatcccg gcgcattcgc gtggatgatg ttgagaactc
     2581 gattgaacca gaccgtggtc tcttcggtcg cagggaaatt cacggcatcc tgtccgttga
     2641 tgtagccgcc gatggccacg ccatcgtatt cagctcgctt cagctccgcg atcaaccgcg
     2701 cgtccccggc ttcgtcgttc gtgagcacga agttggtcgc ctggaacccg agcgagcgaa
     2761 gtttttcctg gacggtggcc gcgatctccg gatgtttgcc gaacccgaga atccgccagc
     2821 ccccgcggcg tgatgaagag gcgatcg
//
LOCUS       DASSLD010000305         2965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_673220,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000305 DASSLD010000000
VERSION     DASSLD010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673220"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..302
                     /locus_tag="VFK23_05510"
     CDS             108..302
                     /locus_tag="VFK23_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET7318577.1"
                     /translation="MFEGLFQPMHLILILFIVLIIFGPGKLPELGEGLGKSIKAFKKA
                     MKEEQASATPIERQREEKKA"
     gene            472..2136
                     /locus_tag="VFK23_05515"
     CDS             472..2136
                     /locus_tag="VFK23_05515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET7318578.1"
                     /translation="MNEPGKDIQDLRTTEWIFQYKRRIISYSLLAVVVSIVAVTITVG
                     AVLRVRLIDDSKAKTRELGEVIRLSFGSLMLAHNPDAIQTTLETIQSRESSVVKAFIL
                     NRKGRVAYSSDKRDIGVVMDRFREESCRGCHVGPEVTPRETTMLLTIDGHEVLRNVEV
                     IYNREPCHVCHAPADRINGKLIIDRSMRPTHALISRVELMLAASGAVCLIVLMPLLSR
                     VLSKGVDKYINEILLKSTELSWLYGIVERWSRTVDMDELRHVVVEVVREVLDADQIDI
                     VFKKEKGEYGGIVWKKADNKTDRRKFPADDPDRPVISTWMTGKLAGEKISLEKSIIYL
                     PIMKGTVPLALIIARKSTGTFDLTGLTLVKAMEGHIAVALENAALHQIAITDELTGLY
                     SQRHFRNFMEKNFVRFERHGEKLALLMIDVDNFKSINDTYGHPAGDQALKAVAQCILS
                     TTRGEDADFRYGGEEFAVILLGTGGSGATFVAERIRQKVESTEFNADAHRFNLTVSVG
                     VAICPENATTIREFVSAADNALYEAKKAGKNRVVLSGAAPGGRSQT"
     gene            2581..2877
                     /locus_tag="VFK23_05520"
     CDS             2581..2877
                     /locus_tag="VFK23_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7318579.1"
                     /translation="MSQIMKKDCCSVMHTDLIEVAAKKMEEKHAGTALVFEEGHISGI
                     ITEKEIKEALAASPSNSELSSEGTMKGEPFIDDGNRCCYFSDVKIELLMGGTRA"
BASE COUNT          765 a          773 c          814 g          613 t
ORIGIN      
        1 gtcgtcaaaa gctgaatctt ccctatccag atatttttcc ctaccgtatt gaaatcgctg
       61 aagaagccgg gtataataag gacattcaaa gaaggagggg tttatttatg tttgagggat
      121 tgtttcaacc gatgcacctg attctcattc tgtttatcgt cctgatcatc ttcggccccg
      181 ggaaactgcc ggaactgggt gaaggcctcg ggaagagcat caaagccttc aaaaaagcga
      241 tgaaggagga acaggcaagc gctacgccta tcgagcgcca aagggaagag aagaaggcgt
      301 aaaccgcccg gcagaaatta tgcgctgcgt caaacgacac ggaccgaaat catacccacg
      361 gttcgtgttt ttttttgtct tcgacaccat ttccccctgt catcctgatc tgaccgaaga
      421 ggctctttgc gcgctcagcg gcaccggggc gaggccgacc tgcggaggga catgaacgaa
      481 cccggcaaag acattcaaga cctccggacc acggaatgga tcttccagta caagaggagg
      541 atcattagct actcgctcct cgcggttgtc gtttccatag tcgccgtaac catcacggta
      601 ggggcagtgc tgcgggtccg tctcatcgac gacagcaagg ccaagacgcg ggaactggga
      661 gaggtgatac gcttgagctt cggcagcctc atgctggccc acaacccgga cgccatccaa
      721 acaacgctcg aaaccataca gtcgcgcgag tcctccgtgg tgaaggcctt catcctgaac
      781 aggaaggggc gggttgccta ctcctcggac aaacgcgaca tcggtgtggt catggaccgg
      841 ttccgggaag aatcctgcag gggctgccac gtcggccccg aggtaacgcc ccgggaaacc
      901 acgatgcttc tcacgatcga cggccatgaa gtgctgcgga acgtggaggt catttacaac
      961 cgggagcctt gtcacgtctg tcatgccccc gcggacagga taaacggaaa actgatcatc
     1021 gaccgctcca tgcgtcccac ccatgcgctc atctcccggg tcgaactcat gcttgccgct
     1081 tccggcgcag tctgccttat cgtgctcatg ccgttgcttt cgcgcgtgct ttccaagggc
     1141 gtggacaagt atattaacga aatccttctc aagtccacgg aactgtcatg gctctacggg
     1201 atcgtggagc gctggagccg gactgtggac atggacgagc tgcgacacgt cgtcgtcgaa
     1261 gtagtccggg aggtcctgga tgccgatcag atcgacatcg tcttcaaaaa ggagaaagga
     1321 gagtacgggg gaatcgtctg gaaaaaagcg gacaataaaa cagacagacg gaagttcccc
     1381 gccgacgacc ccgaccggcc ggttatctcc acctggatga ctggcaagct cgccggggaa
     1441 aagatatcgc tcgagaaaag tattatctat ctgccgatca tgaaggggac ggtccccctt
     1501 gccctcatca tcgccaggaa aagcaccggc accttcgatc tgaccggact caccctcgtc
     1561 aaggccatgg aaggccatat cgccgtggct ctggaaaatg ccgcacttca ccagatcgcc
     1621 atcaccgatg aattgaccgg actctactcg cagcgccatt tccggaattt catggaaaag
     1681 aattttgtgc gctttgagcg ccacggcgag aaattggcgc tcctcatgat cgacgtggac
     1741 aatttcaagt ccatcaacga cacctacggc catccggcgg gcgatcaggc tctgaaagcc
     1801 gtcgcgcagt gtatcttgag cacgaccagg ggcgaggacg ccgatttccg gtacgggggg
     1861 gaggagttcg ccgtcatcct tctcggcacc ggaggaagcg gggcgacatt cgtcgcggaa
     1921 cggatccggc aaaaggtcga aagcaccgaa tttaacgccg acgctcaccg gttcaacctt
     1981 acggtgagcg tcggggtcgc catatgcccc gaaaacgcaa caacgatccg tgaattcgta
     2041 tctgcggcgg acaacgcgct ctatgaggcc aagaaggcgg ggaagaacag ggtcgttctg
     2101 agcggtgccg cgcccggcgg caggtctcag acgtagaaga cgcgcggaac cccggactat
     2161 tagcccgtaa tttccctgca ggaattgtcc caatccacgc agctctccag gttcatcatc
     2221 aataaaaaaa attttcattt cggaacgatc gcgattcccg gcctcttcaa tctttagtac
     2281 tcccaatttt cccgatatag tcacgatccg tcgcatttta ctaaagttaa attcagaaag
     2341 ttagtttggg atgcagagta tttaggaaga atcaggcggg agcaatacta tattattacg
     2401 aatgagaaat caaaaaaatg attacggacg cgctatacta agagagttga acatactccg
     2461 aaaaacgata tttaaataat gaacgaacaa atttttcgtt gatgaatgac aaccgcgttc
     2521 ataacggatg gtcattcatg acttcaaatc gcccggccgg gaagggagga aactatgtta
     2581 gtgagccaga tcatgaagaa ggattgttgc tctgtgatgc acactgattt aatcgaggtg
     2641 gctgcaaaaa agatggaaga aaaacatgca gggacggcgc tcgtcttcga ggagggccat
     2701 atcagcggaa tcataactga aaaggagata aaagaagcgc tggccgcaag ccccagtaat
     2761 tcagaactct cttcggaagg gaccatgaaa ggggaaccgt tcattgatga tgggaacaga
     2821 tgttgttatt tcagcgatgt aaagattgag ttgctcatgg gcggaacccg ggcctgaacg
     2881 gatattcgac aggaaacata cgattgtctc atgtcggggg gatcttggtc ctcgtcagga
     2941 gagctatgtc atttccggga cggtc
//
LOCUS       DASSLD010000306         4195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_719512,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000306 DASSLD010000000
VERSION     DASSLD010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719512"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VFK23_05525"
     CDS             complement(<1..852)
                     /locus_tag="VFK23_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318580.1"
                     /translation="MDNTQDGVEGTLLSERDISEGFDRLIAENRLFGAELNPTTIKKS
                     GKGGLFLFRKLPSRHRMLSLWWDLFSHHISPEALLVELAGGLHWFYHSNILLLFGKDR
                     VIYDCQIRRANEAIGHITLALYRERDRVVPFLPIPTRPGHRVVYIEGITLSAQSTGYA
                     SFLFRRYERLFHELRFHLIRLNAGLSVGKYYWAKEGFDCSNRGQFQQMQDRLWALVRR
                     LSLPVAEQEVRRLPHMSDVAAFRPDLKVRVWRDAEGYYTLEHDTTHPEEFLFPLGKAF
                     LLCAPPWE"
     gene            complement(991..2466)
                     /locus_tag="VFK23_05530"
     CDS             complement(991..2466)
                     /locus_tag="VFK23_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET7318581.1"
                     /translation="MKAWPRHPVIYEINTWVWLADLSRKYRTVMNLATVPEQEWDAIA
                     SHGFDAVWFMGVWERSPAGIEISMRNKGLLEDFRRALPDFTPEDNVGSPYCVRRYVVD
                     EHFGGPKGLEVARNILRERGVRLILDFVPNHVAPDHPWVVDRPEYFVQGSIDDSRNDP
                     ASFVEAGDMVFARGRDPNFPAWPDVLQLNAFQPGLRQAVIGTVSEIAEQCDGIRCDMA
                     MLMLNDVFERTWGARAGAKPVADYWTTVIPAIKGRNQEFRFMAEAYWDLEWELQQRGF
                     DYCYDKKLYDRMQHGDAESVRLHILADSSFQEKMVRFIENHDEPRAAATFPSGKGRAA
                     AVATLTLTGAKLLYEGQFDGLKVRLPVFLGRRPAEPIDQDLAAFYHDLLKTIHHNVFR
                     NGQWRLCERSGWPDNQSCRNILAWCWVKDEERVLIVVNFSDRTSQTLIHVPWDELRGK
                     AWRLTDALSGEVYERSGNKMRDSGLYVDLPPWGYHVFALGS"
     gene            2600..3151
                     /locus_tag="VFK23_05535"
     CDS             2600..3151
                     /locus_tag="VFK23_05535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET7318582.1"
                     /translation="MKQYQIAVVVGSLRKDSFNRKLANAVVRLAPPDFSFKQLQIGDL
                     PLYNQDDDSNQAESVKRLKREITSSQGLLFVTAEYNRSIPGVLKNAIDHASRPYGQSA
                     WAGKPAGVIGISVGAIGTAVAQQHLRNVLAYLDVPTLGQPEVFLQAKDGLFDDDGNIG
                     VISKKFLQNWMDQYVAWVKKHVA"
     gene            complement(3304..>4195)
                     /locus_tag="VFK23_05540"
     CDS             complement(3304..>4195)
                     /locus_tag="VFK23_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HET7318583.1"
                     /translation="PPFRLDLTAWTLRRRPGNIVDRWDGKTYRRALVVGGHPLEVEVE
                     QTGSPDAPRLKIRATSAVQFPGARSAVRNAVERILGARIDLGGFYRFAAGDPALGPVV
                     TRFRGAKPPRFPTLFEALTNAIACQQLSLTVGILLLNRMTEACGAPIPGSDPPVYAFP
                     SPEDVAVLAPETLQSLGFNRQKAHALLELASVIAGKRIDLEGIASLDDDAAVDRLREL
                     RGVGRWTAEYVLLRGLGRIHVFPGDDVGARNHLRRWLGKRNTLGYEEVRSALSRWEPY
                     AGLVYFHLLLKGLSEAGRFP"
BASE COUNT          835 a         1317 c         1250 g          793 t
ORIGIN      
        1 ttcccacggg ggggcgcata agaggaacgc cttgccgagg gggaagagga actcctcggg
       61 gtgggtagta tcgtgctcca gggtgtagta cccctccgca tccctccata cgcggacttt
      121 gaggtccggt cgaaaggcgg ccacgtcgct catgtggggg agccgccgca cctcctgctc
      181 cgccacggga agagagagcc tccgcacgag tgcccagagc cggtcctgca tctgttggaa
      241 ttgcccccgg ttcgagcagt cgaatccctc tttcgcccag tagtatttcc ccacggagag
      301 gccggcattc agtcggatga ggtgaaaacg gagttcgtgg aaaaggcgtt cgtagcgccg
      361 gaagaggaag gacgcgtagc cagtgctttg cgcggagagg gtgatcccct cgatatagac
      421 cacccgatga cccgggcgcg tcgggattgg gaggaacggg acgacccgat cccgctcgcg
      481 gtagagagcg agggtgatgt ggccgatcgc ctcgttcgcc cgccggatct ggcagtcgta
      541 gatcacacgg tccttcccga acaggagaag gatattgctg tggtagaacc agtgcagacc
      601 gccggcaagc tcgacgagca gcgcttccgg ggaaatgtgg tgggaaaaga ggtcccacca
      661 gagagagagc atgcggtgac ggctcgggag tttccggaag aggaacaacc cccccttgcc
      721 gctcttcttg atggttgttg gattgagctc cgcgccgaag agcctgttct ccgcgatcag
      781 ccggtcgaac ccttccgaga tgtcgcgttc cgaaaggagc gtgccttcca ccccatcctg
      841 cgtgttgtcc actgtcagta acccccggtc ataaaccggt tggaacggga tctttcgccc
      901 ccggccatga ccaagcgcgc catccccgcc ttcagtaaac gggttgttgt cacgctcatg
      961 ccgctgctcc ttccggttga ttcgtgtgaa tcagctgccc agggcgaaga cgtggtaacc
     1021 ccagggcggc aggtcgacgt acaggccgga atccctcatc ttgttaccgc tgcgttcgta
     1081 gacttctcct gacaatgcat ccgtgaggcg ccacgccttg cctctcagtt catcccacgg
     1141 cacatggatg agcgtctgtg aggtcctgtc agaaaaattc acgacgatga ggacgcgttc
     1201 ttcgtccttg acccagcacc aggccaggat attccggcag ctctggttgt cgggccagcc
     1261 gctccgctcg cagagacgcc attgaccatt ccgaaagaca ttatgatgga tcgtcttcag
     1321 aagatcgtgg tagaaagcgg cgaggtcctg atcgatcggc tctgccgggc gacggcccag
     1381 gaagaccggc agccgcacct tcaatccatc gaattgcccc tcatatagaa gctttgcccc
     1441 ggtgagcgtg agagtagcca ctgccgcggc gcgtcctttt ccggacggaa aagtggcggc
     1501 agccctcggt tcgtcgtggt tctcgatgaa ccggaccatt ttctcctgga aggaagaatc
     1561 cgcaagaata tgcaggcgca cgctctcggc gtccccatgc tgcatccggt cgtagagctt
     1621 cttgtcgtag cagtagtcga acccccgctg ctggagttcc cactcgaggt cccagtatgc
     1681 ctcggccatg aaccggaact cctgatttct ccctttgatc gccgggatga ccgtcgtcca
     1741 gtaatcagct accggttttg ctcctgcacg agccccccag gtacgctcga agacatcgtt
     1801 cagcatgagc atggccatgt cgcaccggat gccgtcgcac tgttcggcga tttccgaaac
     1861 cgtcccgatc accgcctgcc ggagccccgg ctggaaggcg ttgagctgga gcacatcagg
     1921 ccacgccggg aagttgggat cccggccgcg ggcaaacacc atgtctcccg cctccacgaa
     1981 ggatgccgga tcgttcctcg aatcgtcgat gcttccttgg acgaagtatt caggacgatc
     2041 gacgacccaa gggtggtcag gcgccacgtg attgggaaca aagtcaagaa tcagccgcac
     2101 tccacgttcc cggagtatgt tgcgcgccac ttcgagcccc ttcggccccc caaagtgttc
     2161 gtcaacgacg tagcggcgca cgcagtacgg ggaaccgaca ttgtcctccg gcgtaaagtc
     2221 ggggagggct ctccggaaat cctcgaggag ccccttgttg cgcatggaga tctcgatccc
     2281 ggcagggctc cgctcccaaa cccccataaa ccacacggcg tcaaagccat gggacgcaat
     2341 agcatcccac tcctgctccg gaaccgtggc gagattcatg accgtccggt atttccggct
     2401 caggtcagcc aaccacaccc aggtgttgat ctcgtaaatg acaggatgcc tgggccacgc
     2461 tttcatctct ttgccccacc ggcgactttg aaatcccctc gtcaaactgg tctaaaaacg
     2521 gggggcagtt catccatggc ttgctccatc gtgtatggta cgctctaaat acatcagcca
     2581 cttttataag gagatcgtca tgaaacaata ccaaattgcc gttgtcgtcg gcagtcttcg
     2641 caaagattcg ttcaaccgga agctggcgaa cgccgtcgta aggctggcgc cgccggactt
     2701 ctcgttcaaa cagctacaaa tcggcgactt accgctctac aaccaggacg atgattcgaa
     2761 ccaagccgag tccgtcaagc ggctgaagcg cgaaatcacc tcttcacaag gccttttatt
     2821 cgtcaccgct gaatacaacc gttcgatccc aggggttctc aaaaacgcga tcgaccacgc
     2881 ctcgcgccct tatggccaga gcgcttgggc gggcaagccc gctggtgtga tcgggatttc
     2941 ggtgggcgcc atcggcacag cagtggcgca acagcacctg cgcaatgttc tcgcgtacct
     3001 ggatgtaccg acgcttggcc aacccgaagt tttcctccag gcaaaggacg ggttgtttga
     3061 cgatgacggc aacatcgggg tgatcagcaa aaaattcctg cagaattgga tggatcagta
     3121 tgttgcctgg gtgaaaaaac acgttgcctg aggcggaact ctcattccac ccgccatcaa
     3181 gttccgcctg caccgggccc gtccgtacct gcgcgagcag ctagcccgct acttgcggga
     3241 gggcttcgcg gtatcgacgg tcaggcaact ggacaaaccg agggagtcgg gggcggccca
     3301 acgtcaggga aaccgacccg cttcagagag tcccttgaga aggagatgga agtagacgag
     3361 gcccgcgtac ggctcccatc gggaaagcgc gctgcggacc tcttcgtaac ccagggtatt
     3421 cctctttcca agccagcgcc ggaggtggtt gcgcgctccg acgtcgtcgc cagggaagac
     3481 gtggattcgc cccaggcccc ggagcaggac gtactcggcc gtccagcgcc ccaccccccg
     3541 gagttcccgc agccggtcaa ctgctgcatc gtcatccagg gaggcgatac cttccaggtc
     3601 gatccgtttc ccggcgatga cggacgccag ttcgagaagc gcatgggcct tctgccggtt
     3661 gaaccccagg ctctggaggg tctccggcgc cagcaccgct acatcctccg gactcgggaa
     3721 cgcgtacacg ggcggatccg aaccgggaat cggggctccg catgcctcgg tcatccggtt
     3781 taacaggagg attcccaccg taagggaaag ctgctggcag gcgatggcgt tcgtcagtgc
     3841 ctcgaacaag gtcgggaaac gggggggttt cgccccgcgg aaccgcgtga cgaccggccc
     3901 cagcgcgggg tcgcccgccg cgaaccggta aaacccgccc aggtcgatcc gcgctcccag
     3961 aatgcgctcc acggcattcc tcacggcgga tcgtgcgccg gggaactgta cggcgcttgt
     4021 cgcccggatc ttcagtcgcg gcgcgtcggg ggaacccgtt tgttccactt ccacttccag
     4081 gggatggcct cccaccacca gcgcgcggcg atacgtcttt ccgtcccaac ggtccacgat
     4141 gttgccaggg cgccggcgca acgtccacgc tgtcaggtcg agccgaaaag ggggt
//
LOCUS       DASSLD010000307         5622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_630453,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000307 DASSLD010000000
VERSION     DASSLD010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630453"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFK23_05545"
     CDS             complement(<1..509)
                     /locus_tag="VFK23_05545"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET7318584.1"
                     /translation="MSKVTVLIPAAGAGKRMGKGPAKQFLMLGDKPILAHTLLAFQWT
                     TEIDEIIPILSQEDLETCLGEIIEGYHITKVKTLVVGGKERQDSVANGLHKLEKDSAI
                     VLVHDAVRPFVTPEMIKETVDYAKRGECVAMGVPIKDTIKEVTDQGIVRHTLDRSRLW
                     AIQTPQAFPV"
     gene            complement(1030..1608)
                     /locus_tag="VFK23_05550"
                     /pseudo
     CDS             complement(1030..1608)
                     /locus_tag="VFK23_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
     gene            complement(1640..2476)
                     /locus_tag="VFK23_05555"
     CDS             complement(1640..2476)
                     /locus_tag="VFK23_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7318585.1"
                     /translation="MTNEQWLEVFRSIGRTMCSGIAGFLGREGGTVPLGRGAGGDKTF
                     PVDKWAEDIVVAELEKIYGRGVSFTLISEELGVRTFGEGGKTVLVDPIDGSNNAKSGV
                     PFFSTSLALLDGKRLADLKVGYVIDLSAGDEFWAIRGGGAYKNGARIRTPDTQGITIV
                     AYEASSPGTDIPRLMPLFAQARRTRCFGSTALDLAYLASGAVSVFATATPSRAFDYAA
                     GMLILEEAGGVITDITGAALGTVIAGLERTMPLLAGKNGATHAMALSALASPKGPNPP
                     QY"
     gene            complement(2609..4462)
                     /gene="uvrC"
                     /locus_tag="VFK23_05560"
     CDS             complement(2609..4462)
                     /gene="uvrC"
                     /locus_tag="VFK23_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET7318586.1"
                     /translation="MTLEEKLQNLPNEPGVYLMKDARGHIIYIGKAVSLWNRVRSYFQ
                     KGPKGEKTEMMVRQIADLETIVTHTELEALVLESNLIKKHHPRYNIILRDDKNYPYIR
                     FDLKSEYPRLEVVRRIKKDGALYYGPYVPAGGMWESLALIRRTFPIAPCKIELRADRP
                     GRPCIQFQIGRCTAPCSGEADKAQYRDMVDQVRLFLEGKNRDLLDMLKTRMEEASERT
                     EYERAAELRDRIAKIEGALEKQKIISPGFENQDVIGTAFELGRADIQALFIRNGMLLG
                     RKDFYLDDVRGMTEEEVLSDFLRQFYAKEMIVPQEVLLPIEVADRGTLEAWLAEKRGA
                     KVEVAVPQRGRKRELVQMASDNAAQSLRERLLSKRSKERILLRLQEELGLRNLPRRIE
                     AFDISTIQGAESVASMVSFEDNVPDKRNYKKFRIRTVTGQDDFASMAEVIRRRYSKAK
                     EEGTLPDLILIDGGKGQLNAALDVLRELDIAEPDVIGLAKARSGEEGREQEFERVFLP
                     GVEEPIILEPTSSTTHLVARVRDEAHRFAITYHRQLREKRAIHSELDDIPGIGEVRKK
                     ALLRHFGSVEKIRQAEVEELAAVKGMSGKAAEEIVNHYKGVRAQGTGGRNS"
     gene            complement(4549..5283)
                     /gene="truC"
                     /locus_tag="VFK23_05565"
     CDS             complement(4549..5283)
                     /gene="truC"
                     /locus_tag="VFK23_05565"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HET7318587.1"
                     /translation="MKSLPILFQDDHFVVVNKPAGLLVHRTNIDRHETAYAMQILRDQ
                     LGRWVYPLHRLDKPTSGVLAFALDSEAARRMTDVFSAKNIFKSYLAVVRGFTKDAERI
                     DHPLKERLDRMTDRQADQDKPAQQAVTEYQRLATVELPYAVGRYTTARYSLIKAVPET
                     GRNHQIRRHLKHIFHPVIGDSTYGDGKHNDFFAERFDCRRLLLHAREISFLHPYSGLR
                     ISVQAPFDRAFGDILKTLDWDRVCSA"
     gene            complement(5280..>5622)
                     /locus_tag="VFK23_05570"
     CDS             complement(5280..>5622)
                     /locus_tag="VFK23_05570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012162697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7318588.1"
                     /translation="TPAALEEQYDFITASEVVEHLREPRNELDRLWQCLKPGGTLGIM
                     TKFVLDTDAFPEWHYKNDRTHVCFFSEPTFTWLTGRWGADIAFPANDVVLIMKRAAGR
                     EAAPGREKTAS"
BASE COUNT         1012 a         1709 c         1666 g         1235 t
ORIGIN      
        1 acgggaaagg cctggggggt ctggatggcc cagagcctgc tccggtccag ggtatggcgc
       61 acgatcccct ggtcggtcac ttccttgatc gtgtctttga tcggtacgcc cattgccacg
      121 cactcgcccc gcttcgcgta atcgactgtc tccttgatca tttccggggt cacgaagggc
      181 cgcaccgcgt catgaacgag cacgattgcg gagtcctttt caagtttgtg caggccgttc
      241 gccaccgagt cctgcctctc cttgccgccg acgacgaggg ttttcacctt ggtgatgtgg
      301 tacccttcga tgatttcccc gagacaggtt tccaggtcct cctgggagag gatcgggatg
      361 atttcgtcga tctctgtggt ccactggaat gcaagaaggg tgtgcgcgag aatcggcttg
      421 tcgcccagca tcaggaactg ctttgcgggg cccttaccca tccgttttcc cgctcctgcg
      481 gcgggtatga gaaccgttac cttcgacatg accggacctc gagccggatc aaattccatg
      541 cattcaaaaa gaacacatcc accatggagg cacagagaca cggaaaagag gtgtcagaat
      601 gaaaagggtt cctccgtgcc cctaccttta cgaaccctca cttgtgactc tgtggttaca
      661 ttcgcttttt catgtcatcg aatctcattt cttgaaattt ctcacgagaa tcctgaaccg
      721 cttggagagg ggcgcctcca tattgatcgc gatacgaagc tggaactgtt cctcccgctc
      781 ccccttgatg atgccgaacg gaagaatgat gctgctgtct tccacggagg ccttccccag
      841 gttcaccttc atgcccgcgt cgacggcctc ctcgagggcc gccatctccg gcttggcctc
      901 cgtcatgggc gcacccggca tcgacggctc cgcaggcggc atgtctttca tgacattctc
      961 ggcgggctgg gaccgcttct cgccccggga gacgatatcg ctcagggtga aattgtggcg
     1021 ctcgggattc ttctgcatgt cctgcagtac cagcttggaa atagcggtaa aggtcttcag
     1081 cacgcctgcg ccggtcgtag cgacggcctc gtaaaacggc accttcctcg ggttcaatgc
     1141 cgcgttcaac tcgtcggccg aaagcacgtc gggcaggtcc cgcttgttga actgcatgac
     1201 catggggaat tcggcgagat tgaccccctg ttccgcgaag ttgtcgcgga ggttcgtcag
     1261 gctctccagg ttcgcatcca gcatggcgcg ctgcgagtca gcgacgaaga tgacgccatc
     1321 ggcgcccttc aagaccagct tccgcgtggc gttgtagaag acctggcccg gcaccgtgta
     1381 gaggtggaac cgcactttgt agtttttgat ggtgccgagt tcgacaggaa ggaagtcgaa
     1441 gaacagcgtg cggtccgttt ccgtcgcgag cgaaatgagt ttcccccgct ggttgggcga
     1501 gatccggtga tagatgaggt ggatgttcgt cgtcttcccg cagaggccgg ggccgtagta
     1561 cacaatcttc gcgttgattt cacgcgtggt gtgattgact aaagccatgg tttcatcatt
     1621 acataaaaag aaaaagatgt caatactgcg gtgggttagg ccctttcggg gacgcaaggg
     1681 cgctgagcgc catggcatgc gtcgcgccgt tcttgccggc gagaagcggc atggtccgtt
     1741 caagccccgc gatgacggtc ccgagcgccg ctcccgtaat gtcggtgatg acgccgccgg
     1801 cctcctccag gatcagcata cccgccgcgt aatcgaaggc ccgcgacggc gtggccgtcg
     1861 cgaagacgct gaccgctccc gaggcaaggt aggcgagatc gagcgccgtc gagccgaaac
     1921 agcgcgtgcg tcttgcctgg gcgaaaagcg gcataagccg cgggatgtcc gtccccgggg
     1981 acgacgcctc gtaggcgacg atcgtgatgc cctgcgtgtc cggcgtcctg atccgcgcgc
     2041 cgttcttgta cgcgccgccc ccgcgaatgg cccagaactc gtctcccgcg gaaaggtcga
     2101 tcacatagcc gaccttcaga tcggccagcc tcttgccgtc gagaagcgcc agggacgtgg
     2161 aaaagaacgg gacgccgctt ttcgcgttgt tgctcccgtc gatgggatcc acgagcaccg
     2221 tcttcccgcc ctccccgaag gttctgacgc ccagctcttc agagatgagc gtgaaggaca
     2281 ctccgcgccc gtagatcttc tcgagttcgg cgacgacgat gtcctcggcc cacttatcga
     2341 cggggaacgt cttgtcccct cccgcgcccc gaccgagcgg aaccgtaccg ccctcgcggc
     2401 caaggaaccc ggcgatgccc gagcacatcg tgcggccgat gctgcggaag acttcaagcc
     2461 actgctcgtt cgtcatgatg actccttctc tatattacgg caaatcttta tcgtcattcc
     2521 cgcgaacgcg ggaatccatt ttgaagtttc ttggcagtag ccgatggata cctgctttcg
     2581 caggtatgac agcgtggttc gctctttttt atgaattccg gccccctgta ccctgagccc
     2641 taaccccttt ataatgattc acgatctcct ccgcggcctt ccccgacatc cccttgaccg
     2701 cagccagttc ctcgacctct gcttgccgga tcttctccac gctcccgaaa tggcgcagga
     2761 gcgccttctt ccgcacttca ccgatgccgg gaatgtcgtc cagttcggaa tggatggccc
     2821 tcttctcgcg cagctgccgg tgataggtga tcgcgaagcg gtgtgcctcg tcgcgtacgc
     2881 gtgcgaccag gtgcgtggtc gagctcgtgg gttcgaggat gatcggctct tccacgcctg
     2941 ggaggaacac tcgctcgaac tcctgctccc tgccctcctc cccgctcctg gccttggcga
     3001 ggccgattac gtccggttcg gcgatgtcca gctcccgcag cacgtcgagc gcggcgttga
     3061 gctggccctt gccgccgtcg atcaggataa ggtccggcag cgtcccctct tccttggctt
     3121 tcgaatacct gcgcctgata acctcggcca tgctcgcgaa atcatcctgg ccggtaacgg
     3181 tgcggatcct gaacttcttg tagtttctct tgtccggcac gttgtcctcg aaggacacca
     3241 tgctcgccac ggactcggcg ccctggatgg tcgagatgtc gaaggcctcg atcctcctgg
     3301 gcaggttccg gaggcccagt tcttcctgga gcctcagaag gatccgctcc ttgctccttt
     3361 tggacagcag gcgctcgcgg agcgactggg cagcgttgtc cgaggccatc tggacgagtt
     3421 cgcgctttct gccccgctgc gggaccgcga cctcgacctt cgcgccgcgc ttttccgcga
     3481 gccaggcctc gagcgtcccc cggtccgcga cctcgatcgg caaaaggacc tcctggggaa
     3541 cgatcatctc cttggcgtag aactgccgga gaaaatcgga gagcacctct tcttccgtca
     3601 tgccccggac gtcgtcgagg tagaaatcct ttctaccgag cagcatgccg ttccggataa
     3661 agagcgcctg gatgtccgca cggcccagtt cgaacgccgt gccgatgacg tcctggttct
     3721 cgaacccggg ggagatgatc ttctgcttct cgagcgcgcc ctcgatcttc gcaatccggt
     3781 cccggagctc agccgcgcgc tcgtactcgg tcctctcgga agcctcctcc atgcgggtct
     3841 tcagcatgtc gagaaggtcg cggttcttgc cttcgagaaa cagacgcact tggtccacca
     3901 tgtcccggta ctgggccttg tcggcttctc cggagcaggg ggccgtgcac cgcccgatct
     3961 ggaactggat gcagggcctt ccgggcctgt ccgccctgag ttctatttta cagggcgcga
     4021 tcgggaaggt ccgccggatg agcgccagcg attcccacat cccgccggcg ggcacatagg
     4081 ggccgtaata gagcgcgccg tcttttttta tccggcggac gacttcgagg cgggggtact
     4141 cggacttcag gtcgaagcgg atgtaggggt agttcttgtc gtcccggagg atgatgttgt
     4201 agcggggatg gtgcttcttg atgagattgg actccaggac gagggcttca agctcggtgt
     4261 gggtcacgat agtctcaagg tccgcgatct ggcgtaccat catctcggtc ttctcgccct
     4321 ttggtccttt ctgaaaatag gatcgcaccc ggttccagag agagacggcc ttgccgatat
     4381 agatgatgtg tccccgggcg tccttcataa gatacacgcc gggctcgttc ggcaggttct
     4441 ggagtttttc ttcaagggtc atggcacgtt tattttacca cggataggca gggaggggca
     4501 cggataatat cagcggaagg accggcgagc gtgtccggtt cattgacatc atgccgaaca
     4561 gactctgtcc cagtcgagcg ttttcagtat atcgccaaag gcccggtcaa agggcgcctg
     4621 cacgctgatc cgaagtccgg agtagggatg gaggaatgag atttcccggg catggagcag
     4681 cagcctccgg caatcgaagc gctcagcgaa aaaatcatta tgcttgccgt ctccgtaggt
     4741 gctgtcgcct atgacgggat gaaaaatgtg cttcaagtga cgtcggatct ggtgattgcg
     4801 gcctgtctcg ggaacggctt tgatcaggga atagcgggca gtggtatacc gtcctaccgc
     4861 atagggaagt tcgacggtcg caagccgctg gtattccgtc actgcctgct gcgccggctt
     4921 gtcctggtcc gcttgccggt ccgtcatccg gtccagccgt tccttcaaag gatggtcgat
     4981 tcgttcagcg tccttcgtga acccccgtac aacggcaaga taggacttga aaatattctt
     5041 tgcggaaaag acgtccgtca tccgccgggc cgcctcgctg tccagggcga acgcaaggac
     5101 cccggaggtc ggtttatcga gacggtggag agggtagacc catctcccca gctggtcccg
     5161 caggatctgc atggcgtatg ccgtctcatg acggtcgatg ttcgtacggt ggaccaggag
     5221 gcccgcgggt ttattgacaa cgacaaagtg gtcgtcctgg aacagtatgg gcaatgattt
     5281 catgatgcgg tcttttctcg accgggagcc gcctctcggc ccgccgccct tttcatgatc
     5341 aacacgacat cgttcgccgg gaacgctatg tcagcgcccc accggccggt aagccaggta
     5401 aaagtcggct cggagaaaaa gcagacgtgg gtgcggtcgt tcttataatg ccactcggga
     5461 aatgcgtccg tatccaagac gaatttcgtc atgatgccga gcgtccctcc cggcttgagg
     5521 cattgccata accggtccag ctcatttcgg ggctcgcgca gatgctcgac aacctctgag
     5581 gcggtaataa agtcgtattg ctcttcgagc gcggcaggag tt
//
LOCUS       DASSLD010000308         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_851596,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000308 DASSLD010000000
VERSION     DASSLD010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851596"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            413..2014
                     /gene="sbtM"
                     /locus_tag="VFK23_05575"
     CDS             413..2014
                     /gene="sbtM"
                     /locus_tag="VFK23_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thio(seleno)oxazole modification radical SAM
                     maturase SbtM"
                     /protein_id="HET7318589.1"
                     /translation="MNHRTEKRHTDLYPACRAVLGETGWKHFSRKWADALADQSLSAV
                     LPRTGKDSGPEFLPDLARLEETSFTVLELGKSIPREVAKPHVNPTLRVVKLSWKNLVA
                     FLDPNRKHSTVQPERRSEHVLLWYNPHTGSVSALPADDESLLVLKIVVEGITPESVAA
                     RGKLPVGVVDAALFRAAARGLVLMPPSRIRRDPSFTRSGNGTFEQYLTSSAFTLQWHI
                     TQACDLHCKHCYDRSSRSELKLDQAVRILDDLRAFCMERNVSGAVSFTGGNPLLHPAF
                     TEIYRAAAERGFSTAILGNPSSREQIAELIAIQMPAFFQVSLEGLREHNDSIRGPGHY
                     DRTMEFLEVLRELGVYSMVMLTLTSENISQVLPLAEALRRKTDVFNFNRLSTVGEGAN
                     LKMPDARQYKSFLLSYLDAARGNPVMGVKDNLINIILRDRGMSPFGGCTGYGCGAAFN
                     FASLLADGEVHACRKFPSPIGNVRSQSIAEIYDSDRAQRYRNGSRACQSCAIRPVCGG
                     CLACAHSSGLNVFEDKDPFCFMPDK"
BASE COUNT          505 a          686 c          582 g          456 t
ORIGIN      
        1 tggtacaggc gttaccacgg cagaagcctc aaaagaagaa agttccgata caaaaaaaga
       61 cgaacaggtc tcggcgccga ctccgacagg caacgacacc gaaaccaagg cagaacagcc
      121 taagccggcc ccgtccagcg gctgaagcgg catgaaaacg aaatagtgcg gtctgcacta
      181 tcacagcgca atttcaaaga aatggagtaa cgcggtcccg ggggcggtat ggcaacggat
      241 gaacgctccc gggaactttt ttggtaacta ctcagtcatc tactccggtt gtcattcccg
      301 cgaaagcggg aatccatcgg ctttttctaa gtcagattca aaatggattc ccgataaaag
      361 cattcgggaa tgacggcttg gttttttacg atgcatactt tgtgactgac ccatgaacca
      421 tcggacggaa aaacgtcata cagatttata cccggcgtgc cgggctgttc tgggagaaac
      481 gggttggaaa catttctcca gaaagtgggc cgatgcactg gccgaccagt ccttgtcggc
      541 tgtgctgccg cgcaccggga aggactccgg ccctgaattc ctgcctgatc ttgcccgtct
      601 cgaggaaacc tccttcacag tcctggaact cgggaaatca ataccgaggg aagtagccaa
      661 gccccacgtc aacccgaccc tccgggtcgt caaattatcc tggaagaacc ttgtcgcatt
      721 cctcgacccg aaccggaaac attcaaccgt acagccggag cgaaggagtg aacacgttct
      781 cctctggtac aacccgcaca ccggcagcgt gtccgccttg cctgctgacg acgaaagtct
      841 cctggtgctc aagatagtcg tggagggcat aacgccggag tccgttgcag cacggggcaa
      901 gctgcccgtg ggagttgtcg atgccgctct tttccgcgcc gccgcccgcg gcctcgtgct
      961 catgccgccc tcgcgtatcc gccgggaccc ctcattcacc cgcagcggca acggcacatt
     1021 cgaacagtac ctgacttcct ccgccttcac gcttcaatgg catatcaccc aggcctgcga
     1081 cctccactgc aagcactgtt atgaccggag cagccgttcc gaattgaagc tcgatcaggc
     1141 ggtgcgcatc ctcgacgacc tgcgcgcgtt ctgcatggag agaaacgtct ccggggcggt
     1201 ctcttttacg ggcggcaacc ccctcctcca ccccgcgttc accgaaatat accgtgcggc
     1261 ggcggaacgg ggcttttcca cggcaatcct cggcaatccc tcttcccggg agcaaatcgc
     1321 ggagttgata gcgatccaga tgccggcctt cttccaggta agcctggaag gtctccgcga
     1381 gcacaacgac agcatccgcg gtcccggaca ttatgaccgg accatggagt tcctggaagt
     1441 tttgcgggag ctcggggtat attccatggt gatgctcacg cttacgagcg aaaacatcag
     1501 ccaggtgctg ccgcttgccg aagcgctgcg cagaaagacg gatgttttca acttcaaccg
     1561 tctttcgacg gtgggcgagg gcgcgaatct caaaatgccc gacgcccggc aatacaagtc
     1621 atttctttta tcctatcttg atgcggcccg cgggaacccc gtcatgggcg taaaggacaa
     1681 tctcatcaac atcatcctcc gggaccgcgg catgtccccc ttcggcggct gtacgggcta
     1741 cggctgcggc gcggccttca attttgcgtc tcttctggct gacggcgaag tacatgcctg
     1801 ccgcaaattc ccctccccca tcggcaatgt ccggagtcaa agcatcgccg aaatctacga
     1861 ttcggaccgg gcgcagagat accgcaacgg cagcagggcc tgccaatcct gcgccatccg
     1921 gcccgtctgc ggcggctgcc tcgcctgcgc tcacagcagc ggtctcaatg tctttgaaga
     1981 caaggacccc ttctgcttca tgccggacaa ataagacctg gcatttccgg catcctcatc
     2041 atccaggctt cggtccgttg cacgtcaccg ttcataccct aaaaataccc atgtcggggt
     2101 attgcctctc accgctgaaa gagttattac tataataatt ttgttgcagt attttttatt
     2161 gcctaacctg gataaaccgg aaaagctatc tcgcgcaaag ccgcaaagta cgccaagaaa
     2221 acctttaaa
//
LOCUS       DASSLD010000309         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_19225,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000309 DASSLD010000000
VERSION     DASSLD010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19225"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /gene="ispE"
                     /locus_tag="VFK23_05580"
     CDS             <1..854
                     /gene="ispE"
                     /locus_tag="VFK23_05580"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET7318590.1"
                     /translation="LRSPAKINLCLSVLGKRPDGYHEVEMLMQMVGLFDEVTVKLSGT
                     GIAVNCDAGDVPSGDGNIAWKAASEFLKAVGSSAGFTIEIKKNIPVAAGLGGGSGNAA
                     AVIAAANSLIGAAWGRERLAEIGARVGMDVPFFFFGPTAVARGRGEILTSLPPAPRFW
                     VLLVNPGFETSTAWVYKNINLRLTKKVDCTKIARLTVRNIAEGLHNDLEAVTAAAHPV
                     IRGIEEALLAQQALGVRMSGSGPTVFGIFETESACRAAASNLSSTGWRLFPAEILTES
                     PFAGHLV"
     gene            926..1231
                     /gene="spoVG"
                     /locus_tag="VFK23_05585"
     CDS             926..1231
                     /gene="spoVG"
                     /locus_tag="VFK23_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HET7318591.1"
                     /translation="MDVTEVRVFPVNNEERLKAYVTITIDNVFLIRDLRVINGNAGLF
                     VAMPSRKMKDGTFKDIAHPLNSETRKLIEQKVLTEYERELANPTKREQAPKDAENKQ"
     gene            1390..1463
                     /locus_tag="VFK23_05590"
     tRNA            1390..1463
                     /locus_tag="VFK23_05590"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1422..1424,aa:Gln,seq:ttg)
     gene            1486..>2113
                     /locus_tag="VFK23_05595"
     CDS             1486..>2113
                     /locus_tag="VFK23_05595"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET7318592.1"
                     /translation="MVKLRGQEIKIFSGNANIALAKEICQRMNLPLGNAVVGRFSDGE
                     INVQIVDNVRGMDVFVVQPTSSPVNRHLMELLIMIDALKRASAARITAVLPYYGYSRQ
                     DRKVQPRVPITSKLVADLITTAGANRVLTVDLHAGQIQGFFNIPVDNLFAAPVLLDYL
                     KSKKFNNLTVVSPDAGGVERARAFAKRLGSALAIIDKRRDKPNVAQVMH"
BASE COUNT          479 a          551 c          666 g          417 t
ORIGIN      
        1 cacttcggtc cccggcaaag ataaatctct gcctgtcggt actgggaaaa cgacccgacg
       61 gataccatga ggtggagatg ctgatgcaaa tggtaggtct ctttgacgag gttaccgtga
      121 aactgagtgg aacggggatc gcggtgaatt gcgatgcggg cgatgtccct tccggggacg
      181 ggaacattgc ctggaaggct gccagcgagt tcctcaaggc cgtggggagc agcgcaggat
      241 ttaccatcga gataaaaaag aacatccctg ttgctgcagg cttgggcggc ggtagcggaa
      301 acgcggcggc agtgatcgcc gcggcgaata gccttattgg tgcggcgtgg ggaagggaga
      361 gactggctga aatcggtgca cgcgtgggaa tggatgttcc ttttttcttt ttcgggccga
      421 ctgccgtggc ccgggggagg ggagaaatcc tgacatcctt gccacccgcg ccccgctttt
      481 gggttctgct tgtcaatccg ggattcgaga catcgaccgc gtgggtgtat aaaaatataa
      541 atttgaggtt gacaaaaaaa gtcgactgta ctaaaattgc acggcttacg gtccgaaata
      601 tcgccgaggg gctccataac gacctcgagg ccgtgaccgc ggccgctcat cccgtcatca
      661 gagggattga agaggcgctc ctggcacaac aagcactggg ggtgcggatg agcggaagcg
      721 ggccgacggt cttcggcatc tttgagaccg agtccgcttg ccgggctgcc gcatcaaacc
      781 tctcgagcac ggggtggcgg ctcttcccgg cggaaatact gaccgaatcg ccgtttgcgg
      841 ggcacctggt gtaaacgagg gcgccgtggg tgtgacggcg cgcacctcca tcagaaagca
      901 aggtgacacc acgaggaggt agcatatgga cgttaccgag gtgagggtat ttccggtcaa
      961 taacgaagag cggctcaaag catatgtgac cataacgatc gataacgtgt ttttgatccg
     1021 tgacctgcgt gtgataaacg gaaacgccgg cttgttcgta gcaatgccga gccggaaaat
     1081 gaaggacggg acgttcaaag acatcgccca tccgctgaac agcgagacga ggaagttgat
     1141 cgaacagaag gtccttacgg aatacgagcg ggaattggca aatcccacga agagggagca
     1201 ggccccgaag gacgccgaaa ataagcagta aggaattgaa gcatcccggg gcgggctccg
     1261 ggaatggctc actccaaagt taccattgta agttttgccg attctgaatc gatgcgagag
     1321 gataggcacg cgcctatcct tttgatgtct tatccggttg tcgaaccttg tgcggctttg
     1381 gctcccgatt cggggatcgt ctaatggtag gacagaggcc tttgaagcct tttatcgggg
     1441 ttcgactccc tgtccccgaa ccactgtgct gaaagaaggg gtctgatggt aaaattacga
     1501 ggtcaagaga tcaagatttt ttccgggaat gccaacatag cgctggcaaa agagatctgc
     1561 cagaggatga accttccgct cgggaacgcc gtggtcggcc ggttttccga cggggagatc
     1621 aacgtgcaga tcgtggacaa tgtgcgcggc atggacgtat tcgtggtgca gcccacgtca
     1681 agcccggtca accggcacct catggagctt ttgatcatga tcgacgcgct taaacgggcg
     1741 tcggccgcgc ggatcacggc tgtgctcccc tactacgggt acagcaggca agaccggaag
     1801 gtacagccgc gcgtcccgat cacctcgaag ctggtcgccg atctgatcac tacggcgggc
     1861 gccaaccggg tgcttacggt ggacctgcac gcgggacaga tccagggatt cttcaatatc
     1921 cccgtggaca atctttttgc cgcgcccgtg ctcctcgact atctcaagag caagaagttc
     1981 aacaacctga cggtcgtatc gcccgatgcc ggtggcgttg agcgcgcgcg ggccttcgcc
     2041 aagcggctcg gctcggccct cgcgatcatc gacaagcgga gggacaagcc gaacgtcgcc
     2101 caggtcatgc aca
//
LOCUS       DASSLD010000310         2555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_827683,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000310 DASSLD010000000
VERSION     DASSLD010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827683"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /gene="cdhC"
                     /locus_tag="VFK23_05600"
     CDS             <1..1272
                     /gene="cdhC"
                     /locus_tag="VFK23_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA];
                     GO_function: GO:0043884 - CO-methylating acetyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HET7318593.1"
                     /translation="EVRGLKITVSKPPIPVAFGPAFEGERIRKEDMHIEMGGQRTPAF
                     EWLRTVDLDKVEDGKVTIVGKDADARYQKGGQMPLGMLVEVAGRKMQKDFEPVLERKI
                     HHFVNEAQGIWHMGQRDQNWLRVSKAALAEGYVLRHFGDILATQLKHKFNNIVDKVQV
                     TLYVDDADVKKLLTDAQKAYLERDVRLASMTDESVDTFYSCILCQSFAPNHVCVVSPE
                     RLGLCGAYNWLDAKAAYEIDPNGANQPVLKGETKDAVKGRWAGVDEYVYKNSHQALEY
                     FNAYSIMDAPMTSCGCFECIVAIVPEANGVMVVNRGYTGMTPIGMKFSTLAGTVGGGA
                     QSPGFMGVGRFFLTSKKFLSAEGGFKRIVWMTKNLKESFAEQFKTRAEEEGVPDLLDK
                     IADETVAEDSEKLMAFLTEKGHPALTMESMF"
     gene            1439..1918
                     /locus_tag="VFK23_05605"
     CDS             1439..1918
                     /locus_tag="VFK23_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET7318594.1"
                     /translation="MEEKLEGIYARYRENEGNLISILQDIESEFGYLREDAINWFSER
                     LDVPPARFYGVATFYAQFHLKPRGKNIITACCGTACHVKGSERLINSLLKELAIPSGE
                     ETSEDHKFTVEKVNCVGACSIAPVIIVNKAVHGKMSPDKLMKEIKTLKAAEAEHPKS"
     gene            1998..>2555
                     /locus_tag="VFK23_05610"
     CDS             1998..>2555
                     /locus_tag="VFK23_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HET7318595.1"
                     /translation="MNKDIAVKVCMGTGGMAAGGEQVMSSFEKAFAEAGITAVVQKNC
                     SVHKVGCRGFCARDVLVDVIIGGVKSTYQYIKPDMVERIVTEHVVEGKPVAEWLVTDD
                     YHTFHNKQIKVVLSDCGEIDPEDIDSYIARGGYEAVKNVLATMAPQEVIEVIKASGLR
                     GRGGAGFPTGTKWDMAIKAPGPVKYL"
BASE COUNT          579 a          734 c          808 g          434 t
ORIGIN      
        1 gaggttcgcg gcctcaaaat tacggtctcg aagccgccca tcccggtggc cttcggcccg
       61 gcgttcgaag gcgagcgcat ccgcaaggag gacatgcaca tcgagatggg cgggcagcgc
      121 acgccggcct tcgagtggct ccgcacggtg gacctggaca aggtggagga cggcaaggtg
      181 accatcgtgg gcaaggacgc cgacgcccgg taccagaaag gcggccagat gccgctgggc
      241 atgctggtcg aggtcgcggg ccgcaagatg cagaaggact tcgagcccgt tctcgaacgc
      301 aagatccacc atttcgtcaa cgaggcccag ggcatctggc acatgggcca gcgcgaccag
      361 aactggctcc gcgtgagcaa ggccgcgctc gccgaaggct acgtcctcag gcacttcggc
      421 gacatcctgg cgacccagct caagcacaag ttcaacaaca tcgtcgacaa ggtccaggtc
      481 accctctacg tggacgacgc cgacgtgaag aagctcctga ccgacgccca gaaggcctac
      541 ctcgagcgcg acgtgcgcct ggcatcgatg acggacgagt cggtggacac cttctactcc
      601 tgcatcctct gccagagctt cgcgccgaac cacgtgtgcg tcgtatcgcc ggaacggctc
      661 ggcctctgcg gcgcctataa ctggctcgac gccaaggcgg cgtacgagat cgacccgaac
      721 ggagcgaacc agccggtcct caaaggcgag accaaggacg cggtgaaggg gcgctgggcg
      781 ggggttgacg aatacgtgta caagaactct caccaggcgc tcgaatactt caacgcctac
      841 tccatcatgg acgcgcccat gacctcctgc gggtgcttcg aatgcatcgt ggccatcgtg
      901 cccgaggcga acggcgtgat ggtagtgaac cgcgggtata ccggcatgac gccgatcgga
      961 atgaagttct cgaccctggc cggcaccgtg ggcggcggcg cccagtcgcc gggcttcatg
     1021 ggtgtgggcc ggttcttcct cacgagcaag aagttcctct cggccgaggg cgggttcaag
     1081 cgcatcgtct ggatgaccaa gaacctcaag gagtcctttg ccgagcagtt caagacacgg
     1141 gcggaggaag agggcgtgcc cgatctcctg gacaagatcg ccgatgaaac ggtagcggag
     1201 gattcggaga agctgatggc gttcctcacg gaaaagggcc atccggcgct cacgatggaa
     1261 tcgatgttct aactcctgaa aactggtagc gtaacgatgc gccccttcgg gggcgcatga
     1321 cttctcgaag gccggatgcg caggttattg ctggcaccac gaccgcgatg tggaaaggct
     1381 cagacttggc tgccggaata aattgaaggc ggccattttt gtctcaaggg gtttacacat
     1441 ggaagaaaag ctcgagggaa tatacgcccg ataccgggaa aatgagggga acctgatctc
     1501 gatcctccag gacatcgaga gcgagttcgg ctacctgcgg gaggacgcga tcaactggtt
     1561 ctcggaacga ctcgacgtcc cgccggcgag gttctacggg gtcgccacgt tctatgccca
     1621 gttccacctg aagccccggg ggaagaacat catcaccgcc tgctgcggaa ccgcgtgcca
     1681 cgtaaagggt tccgagcggc tcatcaacag tctcctgaag gaactggcca tcccgtcggg
     1741 agaggaaacg tccgaagacc acaagttcac cgtggaaaaa gtgaactgtg tcggcgcctg
     1801 cagcatcgcg ccggtcatca tcgtcaacaa ggcggtgcac ggcaagatgt ctccggacaa
     1861 gctcatgaag gaaatcaaga cactgaaagc ggcagaagcg gagcatccta aatcctgaat
     1921 taggatgctc aagacccaac agtgtcggat gacgcacatg caagacaatg aataagaatt
     1981 tgtcttctgg agcaatcatg aataaagata ttgcagtaaa agtttgtatg ggcacgggcg
     2041 gaatggcggc cggcggcgag caggtgatgt cctcgttcga gaaggccttt gccgaagcgg
     2101 gcattacggc agtcgttcag aagaattgct ccgtacacaa ggtgggatgc cgcggcttct
     2161 gtgcacgcga cgtgctggtg gacgtgatca tcggcggcgt caaatccacg taccagtaca
     2221 tcaaaccgga catggtcgag cggatcgtca ccgaacacgt cgtggaaggc aagcctgttg
     2281 ccgagtggct cgtaaccgac gactaccata ccttccataa taagcagatc aaggtggtcc
     2341 tgtccgattg cggcgagata gaccccgagg atattgactc ctatatcgct cgcggcggct
     2401 acgaggctgt gaagaacgtg ctcgcgacca tggccccgca ggaagtgatc gaagtcatca
     2461 aggcctcggg tcttcgcggc cgcgggggcg cggggttccc gacgggaacg aagtgggaca
     2521 tggcgatcaa ggctcccggc cccgtcaagt atctc
//
LOCUS       DASSLD010000311         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1053761, whole genome shotgun sequence.
ACCESSION   DASSLD010000311 DASSLD010000000
VERSION     DASSLD010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053761"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2019
                     /locus_tag="VFK23_05615"
     CDS             <1..2019
                     /locus_tag="VFK23_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318596.1"
                     /translation="YGTTFTFMQWLVKTLDKTDTGYALFTLASSAIAMFVMLPATFCA
                     GMTLPLITFILIRGGHGERSIGAVYAANTVGAIIGVFFAVHLGMPLLGLKNLITCGAG
                     LDIALGVGLFWFSLAESGDRRLPVIATVTGVCAVAATIFLVQLDPYKMGSGVYRTGEI
                     ETPEGFTVLYHKDGKTATVNCFQRKDGLTSIRTNGKIDASIMMARGSVVSPDESPMIL
                     LAAIPMSLHPRAVTAAAIGLGSGLTSHTLLSNPGLQQVDTVEIEKGMIEGANYFRPRV
                     ESVFSDPRSRVINDDAKTFFSTAGKKYDLIVSEPSNPWVSGVAGLFSTEFYRLIKRHM
                     HDDALFVQWLQIYEINVDLVASVLKAISANFSDFVVYASNNSDIMVIARKNGQVPTPA
                     AGVFEIPAIAEALKRIHIEGVQDIEVRRVGNKRIYSRLLETFPIQENSDYYPVLDQDA
                     GRVRFLGSTANELMSLRFTMTMLSDARGQKETANITPSPDFEFSRKTLTAMGLRDYFL
                     HGSFGGRYVPADLRQKAESLKRMCGGGAAGDANHRLNLEYSMADAMSFFLTSSDMEEV
                     WKTLSRGPCDALASPEERAWFRLFRAIGTHDAGKMLEDAKSFLAGGNDLPPGLKKYLL
                     AVGMLGAISQGQPEEAARLWSVYGGGLPDGSSESTLLFRMLAAESSRP"
     gene            complement(2082..>2988)
                     /locus_tag="VFK23_05620"
     CDS             complement(2082..>2988)
                     /locus_tag="VFK23_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET7318597.1"
                     /translation="GLGRTLAATAVDGVTLTVGRGEAVGLVGESGCGKSTLGRLALRL
                     LEPTSGAVRFEGKDLAALDKQELRELRKNMQIIFQDPFASLNPRMRILDIINEPLVIH
                     GLARKNELQKKGAALLEKVGLTADALRRYPHEFSGGQRQRIGIARALASGPSFIVADE
                     PVSALDVSVQAQIINLLEDLRDEMKLSFLFIAHDLNIVRHFSDRVVVMYLGKVMESAP
                     SEEIYRNPSHPYTEALLADIPVPDPTVKKKHILLKGDIPSPTHIPPGCRFHTRCIYRF
                     DPCDKIVPRTTDLGGGHFVDCHLRG"
BASE COUNT          594 a          843 c          912 g          639 t
ORIGIN      
        1 tacggcacaa cttttacctt catgcaatgg ctcgtgaaaa ctctcgacaa gacagacacc
       61 ggctacgccc tcttcaccct ggcgagcagc gccattgcca tgttcgtcat gctgccggcg
      121 accttctgcg cggggatgac cctgccgctc ataacgttca tccttatcag gggaggccac
      181 ggagagcgaa gcatcggcgc ggtgtatgcg gccaataccg tcggagcgat catcggcgtc
      241 ttttttgccg tccacctcgg gatgccgctt ttggggctga agaacctcat cacgtgcggc
      301 gcaggcctcg acatcgccct cggcgtggga ttattctggt ttagtctggc ggagtccggc
      361 gaccgccgcc tgccggttat cgcgaccgtg accggcgtat gcgcagttgc tgctacaatc
      421 tttcttgtgc agcttgatcc ctataaaatg ggatcagggg tgtaccggac aggcgaaatt
      481 gaaactccgg aaggatttac ggtgctgtac cataaggacg gaaagacagc cacggtcaat
      541 tgtttccaga ggaaggacgg gttgacgtcg atccgcacca acgggaaaat cgatgcctcg
      601 atcatgatgg cacggggaag cgtggtctcg cccgatgaat cccccatgat cctgcttgcg
      661 gcaattccga tgtcccttca cccccgggcc gtaaccgccg ccgctatcgg gctcgggtcc
      721 gggctcacct ctcatacgct gctctctaac cccgggctgc agcaggtcga caccgtggag
      781 atcgaaaagg gaatgatcga aggcgcgaac tatttccggc ctcgtgtcga gtcggtgttc
      841 agcgacccgc gcagcagggt tatcaacgac gatgccaaga ccttcttttc gaccgccggt
      901 aaaaaatacg acctgatcgt gtccgaaccg tcaaacccct gggtcagcgg tgttgccggc
      961 cttttttcca cggaattcta ccgcctgatc aaacggcaca tgcatgatga tgctctgttc
     1021 gtgcagtggc tgcagattta tgaaataaac gtggacctgg tcgcttccgt actgaaggcg
     1081 atctcagcca atttttccga cttcgttgtt tatgcttcca acaacagcga tattatggtc
     1141 atagcgagaa aaaacggcca ggtgcccacc ccggccgccg gcgtcttcga aatacccgcg
     1201 attgccgagg ccttgaaaag gattcatatt gaaggcgtcc aggacattga agttcgccgg
     1261 gttgggaata aacgcatcta ctcccgtttg ctcgaaacct tccccattca ggaaaattcg
     1321 gattattatc cggtgctgga ccaggatgca gggcgtgtgc gtttcctcgg cagtaccgcg
     1381 aacgagttaa tgagtctccg ttttactatg accatgttgt ccgatgcgcg ggggcagaag
     1441 gaaacagcga acatcactcc atccccagac ttcgaatttt cacggaagac cctcacggcc
     1501 atgggcctgc gagattactt tctccatggc agtttcggcg ggcgttatgt cccggcggat
     1561 ttgcggcaaa aagccgagtc attgaagcgg atgtgcggcg gcggcgccgc cggcgatgcg
     1621 aatcaccgcc tgaacctgga atatagtatg gccgatgcca tgagcttttt cctgacctct
     1681 tccgacatgg aggaggtatg gaagacgctg tcaagggggc cgtgcgatgc gctggcctct
     1741 ccggaggaga gggcgtggtt cagactattc agggccatcg gtacgcacga tgccggtaaa
     1801 atgctggagg atgcaaaatc atttttagca ggtggtaatg accttcctcc aggcctgaag
     1861 aaatacctcc ttgccgtggg catgctggga gcgataagtc agggacagcc agaggaagcc
     1921 gcccgtttat ggtccgtata cgggggaggg ctgcctgacg gaagcagcga gtccacactg
     1981 ctgtttcgaa tgctcgcggc tgagagctcc cgcccatgat gcaggtgcat cgaggatgcc
     2041 tgcagtggca taatgcagcg ggttcgcttc gatgcggtgc attacccccg caggtggcag
     2101 tctacgaaat gtccgcctcc gaggtcggtg gtgcgcggta cgattttgtc gcaggggtcg
     2161 aaacggtaga tgcagcgggt gtggaagcgg cagccgggcg ggatgtgcgt ggggctcggg
     2221 atgtcgccct tgagcaggat gtgctttttt ttcaccgtgg ggtccgggac cgggatgtct
     2281 gcaagcaagg cctcggtgta agggtgagaa gggttgcggt agatctcctc ggaaggggcg
     2341 ctctccatga ccttccccag atacatcacg acgacgcgat cggaaaaatg gcgcacgatg
     2401 ttgaggtcat gggcgatgaa caggaaggag agcttcattt cgtctcgcag gtcttcgagg
     2461 aggttgatga tctgggcctg tacggagacg tcgagtgccg agacgggctc gtccgcgacg
     2521 atgaatgacg gcccggaagc cagcgccctc gcgatgccta ttctctgccg ctgaccgccg
     2581 gagaattcat gcgggtaccg ccggagcgcg tcggccgtca gccccacctt ctcgaggagc
     2641 gcggcgcctt ttttctgaag ctcgtttttc cttgccagcc cgtggatcac gagcggttcg
     2701 ttgatgatat caaggatgcg catgcgcggg ttgagcgacg cgaaggggtc ctggaagatg
     2761 atctgcatgt tcttccggag ttcccgcagt tcctgtttat caagggcggc caggtctttt
     2821 ccctcgaagc gcacggcccc ggacgtgggc tccagcagcc tgagcgcgag cctgccgagc
     2881 gtagacttgc cgcatccgct ctctccaacg aggcccacag cctcgccccg tccgacggtg
     2941 agcgtgacgc cgtcaactgc cgtcgcagcg agcgtcctgc ccagcccc
//
LOCUS       DASSLD010000312         3615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1414257, whole genome shotgun sequence.
ACCESSION   DASSLD010000312 DASSLD010000000
VERSION     DASSLD010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414257"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /gene="ettA"
                     /locus_tag="VFK23_05625"
     CDS             <1..1263
                     /gene="ettA"
                     /locus_tag="VFK23_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HET7318598.1"
                     /translation="NAWELDSRLEMAMDALRCPAGDTPVKVLSGGEKRRVALCRLLLQ
                     KPDILLLDEPTNHLDAESVAWLEHHLNSYEGTVIAVTHDRYFLDNVAGWILELDRGQG
                     IPWKGNYSSWLEQKQERLKREEKSESERQKTLQRELEWIRMSPKGRHAKSKARISSYE
                     ALLSQQAEKQAKELEIYIPPGPRLGKVVIEADAVTKAYSDNILVENMSFMLPPGGIVG
                     VIGPNGAGKTTLFRMITGQEKPDKGTIRIGETVKLGYVDQSRDALDPNKTIWQVITDG
                     QEQVQLGKSLMNSRAYVSRFNFSGTDQQKKVGVLSGGERNRVHLARILKEGANVLLLD
                     EPTNDLDVNTMRALEEGLENFAGCAVVISHDRWFLDRIATHILAFEGESKVVFFDGNY
                     SEYEADRKSRLGADADQPHRIKYRHLTR"
     gene            1299..1718
                     /locus_tag="VFK23_05630"
     CDS             1299..1718
                     /locus_tag="VFK23_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318599.1"
                     /translation="MMQNGSSGETVRTFNQGTAHGSHRMSGRPSALGEGDPIEENGNG
                     KKYISDHILPTAANLLGLCFVILSFIKVMKIGAETAIDELVAVAIVIFLVASIFSYAS
                     IRSETHSDRFEKTADIVFIAGLGLLTVTAIVVVFEIV"
     gene            2040..3428
                     /locus_tag="VFK23_05635"
     CDS             2040..3428
                     /locus_tag="VFK23_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7318600.1"
                     /translation="MSRTVEITSDVLPRLDRLPWSRWHLRVVSALGFGWLLDSLEANI
                     IGSVLGILKDLWKFSPLQGSLTVSAWLIGVMVGAVVFGYLSDRSGRKKIFIITLVWYG
                     LFTLACAFSTNIWVFIFLRFMAAVGIGGEYAAISSAVVEFVPRNVRGRTDAFIMSLWP
                     VGAVCSALLVMFALSLFPPQIAWRAGFLLALVLALFALYVRRRLPESPRWLLERSRFE
                     EAQAVVAAAEKEVMKSKGLTELPPAPPITIRQGEHGVWHETRLLYSKYLARVALGSAL
                     NFAQVALGYGSIAFASLVLFPMTNTPPDRVPFFMMIAFLFAFLGGLTSVFMVDTLGRK
                     ATGILSYGSYLIAGFSMLFASTPFSALVALCIMQFCYTWGWITEYVIKSEIFPTHVRA
                     AGIGWATFFGRIGGVIAAPVLTSVYQSTGSIATVAFVFAAIVSPGFIAAVFWAVKGVE
                     GKRKALEELLKD"
BASE COUNT          682 a         1093 c         1101 g          739 t
ORIGIN      
        1 aacgcctggg aactggattc ccgactcgag atggccatgg acgcgctccg ctgtcccgca
       61 ggcgatacgc cggtcaaggt gctctcgggc ggcgagaaaa ggcgcgtagc gctttgccgc
      121 cttctccttc agaagccgga catcctgctc ctggacgagc ccacgaacca cctggacgcc
      181 gagtccgttg cctggctcga acatcatttg aacagctacg agggcacggt catcgccgtg
      241 acccacgacc gctacttcct cgataatgtt gcgggctgga tcctggagct cgaccggggc
      301 cagggcatac cgtggaaggg gaactattca tcatggctcg aacaaaaaca agagcgctta
      361 aagcgggagg agaagtcaga gagcgaacgg cagaagaccc ttcagaggga gcttgaatgg
      421 atcaggatgt ctcccaaggg caggcacgcg aagtcgaagg cgcgcatcag ctcctacgag
      481 gccctgctct cccagcaggc ggagaagcag gcgaaagagc tcgagatcta catcccgccg
      541 ggaccccgtc tcggcaaggt cgtgatcgag gcggacgctg tgaccaaggc ttacagcgac
      601 aatattctcg tcgagaatat gtctttcatg ctcccgccgg gcgggatcgt gggcgtgatc
      661 gggccgaacg gcgcgggaaa gaccacgctc ttccgcatga tcacgggcca ggagaagccg
      721 gacaagggga cgatcaggat cggcgagacc gtgaagctcg ggtacgtgga ccagagccgg
      781 gacgcgctcg atcccaacaa gaccatctgg caggtgatca ccgacggcca ggaacaggtc
      841 caactcggca agtccctcat gaactcacgc gcctatgttt cacggttcaa cttttccggc
      901 acggaccagc agaagaaggt gggcgtgctc tcgggcggag agcgtaaccg ggtgcatctc
      961 gcgcgcatcc tgaaggaagg ggcgaacgtg cttcttctgg atgagccgac gaacgacctc
     1021 gatgtaaaca ccatgcgggc gctcgaagaa ggccttgaga acttcgcggg ctgcgccgtc
     1081 gtgatctccc acgaccgatg gtttctggac cgcatcgcca cgcatatcct tgcattcgaa
     1141 ggggagagca aggtggtctt cttcgacggg aactactccg agtacgaggc ggaccgcaaa
     1201 tcacgtttgg gcgcggacgc ggaccagccg caccggatca agtaccggca tctgacgaga
     1261 tagtgaagcc ccggggcctc gggtgtgagc atgaggggat gatgcagaac ggaagctccg
     1321 gcgagaccgt ccgcacattc aaccaaggaa cggcgcacgg gtctcataga atgagcggcc
     1381 ggccatcagc gctcggtgaa ggggatccca ttgaggaaaa cggaaacggc aagaagtata
     1441 tttctgacca tattttgccc acggcggcaa acctgctggg actttgcttc gtcatcctga
     1501 gtttcatcaa ggtcatgaaa atcggcgccg aaacggcgat cgatgagctg gtcgctgtgg
     1561 ctatcgtcat ctttctggtt gcaagcatct tctcctacgc atccatccgg tcggagacgc
     1621 attcggaccg gtttgagaag accgccgata tcgtcttcat tgcggggctg ggcttgctca
     1681 cagtaacggc cattgtcgtc gtgttcgaga tcgtttaatt cctccctgct gccgtctccc
     1741 ctgacgagcg catcctcgat ccgtccccgg caggcagctg caagatcatt catgtcgtga
     1801 aagttaagcg gctcttgagc ctcgcgaatg atccggcccc acagctccct caacgcacag
     1861 gaacgtctct ctcccgtgtg ttaaactaag agcagaggga acccgcagct gtcggagaga
     1921 cgctccttca taagggttgc tgcttcgtcc tcactggttt ttcccgtcca tcatcgtaca
     1981 gctgcccgtc tccgcgctga acgccgtatt ccccgggttt gggatttgca ttttcagaaa
     2041 tgtccaggac cgtcgaaata acaagcgatg tcctgccccg cctcgaccgg cttccctggt
     2101 cgcggtggca tctgcgcgtc gtgtccgctt tgggattcgg ctggctgctg gattcgctcg
     2161 aggcgaacat catcggcagc gtgctaggca tcctgaagga cctctggaag ttcagcccct
     2221 tgcagggctc ccttactgta agcgcctggc tcatcggggt catggtcggc gcggtcgtgt
     2281 tcgggtatct ttccgaccgc tccggccgga agaagatatt catcataacc cttgtctggt
     2341 acggtctctt caccctggcc tgcgcttttt ccacgaacat ctgggtcttc atcttcctga
     2401 ggttcatggc cgccgtcggc atcggcggcg agtatgcggc catcagctcc gcggtcgtcg
     2461 agttcgttcc gaggaacgtg cgggggagga cggacgcctt tatcatgtcc ctgtggccgg
     2521 tcggcgcggt ctgttccgca ctgctcgtga tgttcgcatt gagcctcttc cccccgcaga
     2581 tcgcgtggcg ggccggcttc ctgcttgccc tcgtcctggc cctgttcgcg ctctacgtcc
     2641 gccggcgtct ccccgaatcc ccccgctggc tgctggaacg ctcccgcttc gaagaagcgc
     2701 aggccgtcgt cgctgccgcc gagaaggagg tcatgaagag caaggggctc acggaactgc
     2761 cgccggcgcc tcccatcacg atccggcagg gcgagcacgg cgtgtggcac gagacccggc
     2821 tgctctacag caagtacctt gcgcgggtgg ctctcggctc ggcgctgaac ttcgcgcagg
     2881 tggcgctggg gtacggctcc attgctttcg cctccctggt ccttttcccg atgacgaata
     2941 cgccgcccga ccgcgttccc ttcttcatga tgatagcttt cctgttcgcc ttcctcggcg
     3001 gcctcacctc cgtgttcatg gtcgataccc tggggcgaaa ggcgaccggg atcctttcgt
     3061 acgggtccta ccttatcgca ggcttcagca tgctcttcgc gagcacgccc ttctcggcac
     3121 tggtcgccct ctgcatcatg cagttctgct acacctgggg atggattacc gagtacgtga
     3181 tcaaatcgga gattttcccg acgcatgtcc gggccgcagg gatcggatgg gcgaccttct
     3241 tcggaaggat cggcggggtc attgccgcgc cggtcctgac gagcgtatat caatcaaccg
     3301 gatcgatcgc caccgtcgcg ttcgtgttcg cagccatagt ctccccgggg ttcatcgcgg
     3361 ccgtcttctg ggccgtgaaa ggggtcgaag ggaagaggaa ggctttggag gagctgctca
     3421 aagactgacg tcgagccgcg tcatcgggga aaagcgggat ggggagggca tcgggaagga
     3481 agtgaagaag ctatgaacgt gccgtgacgc gccgccttga ggcaggcgcg ccgccctcga
     3541 tctgcctcgt tagaaccatc gattcatcgt tgatctgttc tgaccgtccg actttattta
     3601 tttttcggct ccagc
//
LOCUS       DASSLD010000313         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1076300, whole genome shotgun sequence.
ACCESSION   DASSLD010000313 DASSLD010000000
VERSION     DASSLD010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076300"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..983)
                     /locus_tag="VFK23_05640"
     CDS             complement(159..983)
                     /locus_tag="VFK23_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7318601.1"
                     /translation="MKISVIFTTYNKPDYLRLCILSFAQQTLLPSEVIVADDGSTEET
                     GALIHEMKKRFSNLFTIKHVWHEDVGFRRSGILNKAAREASGDYLIFTDGDCMAHRHF
                     VRSHVEYSHPSAVLGGRRIEIGKELTERLLGEGSIVNSFNMRLLYDAIKGQSRKVEEA
                     IHIKNNLLRWMLHRDRITDDGIWGCNCSLYKKLFMEVNGYDEDFQYSNEDNDLGIRAL
                     NQGKNVKSVRGLAIVFHLWHPTRWNFEDERFYRDYAILERRIANREPFCKNGVIKK"
     gene            complement(1146..2048)
                     /gene="lipA"
                     /locus_tag="VFK23_05645"
                     /pseudo
     CDS             complement(1146..2048)
                     /gene="lipA"
                     /locus_tag="VFK23_05645"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
     gene            complement(2151..>3159)
                     /locus_tag="VFK23_05650"
     CDS             complement(2151..>3159)
                     /locus_tag="VFK23_05650"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HET7318602.1"
                     /translation="EVLDPEQNKDFRDNYLDVPFDLSKVMFITTANQLETIPPPLLDR
                     MEVLHLPGYTEHEKLRIAQKYLIPKEIKANALKPEEIVIEEDALRGIIKEYTREAGVR
                     NVEREIANVCRKVAKAVAEGKPTPITVKVGDLHDYLGNPRFFAETAIMIDRPGVVTGL
                     AWTPTGGDILFVEATQMPGNKQLTLTGQLGDVMKESAQAALSYVRSQAETFGINRDFF
                     EKTDIHIHVPAGAIPKDGPSAGVTMTTALVSMLTGKPARHDLAMTGEITLRGKVMPIG
                     GVKEKVLAAKRAGIKAVILPEKNKNDLEDVPEDLRKEMQFIFVDTIDQVISNALDSKG
                     K"
BASE COUNT          678 a          871 c          793 g          817 t
ORIGIN      
        1 tgctaagaat gtattaagaa ttccttgttc tttcggcaaa cttatgcctt cttcctcggc
       61 aaccttatgg gtaaattcat acccaccaca atccaccacc gcatcacata cgtaatgtcc
      121 cacttctcca gcttcgatgt aaccttttcg tttgcaggtt actttttgat tacgccgttt
      181 ttgcagaatg gctctctgtt cgcgatacgt ctttcaagaa tggcataatc acggtaaaat
      241 ctttcatctt cgaaattcca ccgggtagga tgccaaaggt gaaaaacgat tgcaagtcct
      301 cgtacggatt taacgttctt gccctgattc agcgcccgaa tgccgaggtc gttgtcttcg
      361 ttggaatatt gaaaatcctc atcgtaaccg tttacttcca tgaagagctt tttatacaga
      421 ctgcagttgc aaccccagat cccgtcatcg gtaatccggt cccggtgaag catccatcgc
      481 agaagattat ttttaatatg gattgcctcc tcaacttttc gggattgtcc cttgatcgcg
      541 tcgtacagca gcctcatatt aaatgaattg acaatcgaac cttcacccaa tagccgttcg
      601 gtcagttctt ttccaatctc gatacgtctc ccgccaagaa cggccgaagg atggctgtat
      661 tccacatggg aacggacaaa atgacggtgg gccatgcagt ccccatcagt aaaaatcagg
      721 taatcgcccg atgcttcacg cgccgccttg ttgagaatac ctgaccgccg gaaaccgaca
      781 tcctcgtgcc acacatgctt aatcgtgaaa agattgctga atcgtttttt catttcgtga
      841 atcaatgcgc cggtttcttc ggtactaccg tcatccgcca caattacttc cgacggcaag
      901 agggtttgtt gggcaaagct gaggatgcac agtctcaggt aatcgggttt gttgtaagta
      961 gtaaaaatca cggatatttt cataggaatg taattataaa taattgcatt ttaaaagtcc
     1021 agagtccaga tgaacgaaaa tttttcactt tactctctaa ggctgctcta ttcatgagga
     1081 attaaaacgg tatgctgtat gattgggcag ggacatgaaa atacaataaa caggggagga
     1141 aaaggttatt tacctttttt cgataagtca atagttcccc cgatctccgg gattccatat
     1201 ttctcagcat ggtaagagct cctgatgagc ggtcctgcca ctacgcggct gaaccccata
     1261 cccaatgccc ggctgcgaag atcttcaaat tcatcgggat gatagtattt tttaaccgtg
     1321 agatgattct tccccggctg gagatactga ccgatcgtca cgatcgagca accagcctgc
     1381 cgcgcgtcct gcagcacggt cgtgatctcc tcaattccct cccccagccc gagcataagc
     1441 cctgttttgg tgatcgcgcc gaaatcacgg gcgagcctga gcacttttag cgagcggcca
     1501 taattcgcct gaggtcgtac ccttgaatag agtgacggga cagtttcgag attgtggttc
     1561 agtacatcgg gctttgcatc cagaactttc tggagcgacg gttcagatcc ctgaaaatcg
     1621 gggataagaa cttcaatgcc gcacccggga gtggtttcac gaatggctcg gatcgtatcg
     1681 gcaaagatac cggcgcctcc gtcaagcaga tcatcacgcg taaccgaggt gatcaccgca
     1741 tagcgcaggt gaagagcgga tatcgctctc gctatgcgat ttggttcgtc tcgatcaagc
     1801 ctttcgggca gtcctttggg tacattgcag aacccgcagt tccgggtgca gacgtttccc
     1861 aggatcatga acgtcgccgt acccgcattc cagcactcca tcctgttcgg gcatgctgcg
     1921 ctcatgcaga ccgtatggag atggttgctg ctcacgaggt tttgcacctt cgctgaatta
     1981 tttcccacgg tcaaccgaat cttgaaccag gagggaagcc gccgagtggc cgattgtgaa
     2041 ggcatgttgt gattctcgtc tgactgccgg tcacgtcatt cgccccggat gttgccgctc
     2101 acctcacggt acaggcctcg cagccggtcg acgaaagacg tgacgagaac ttatttccct
     2161 ttgctgtcca gcgcgttgct gatcacctgg tcgatcgtgt ccacgaagat gaattgcatc
     2221 tccttgcgaa ggtcttcggg cacgtcctcg aggtcgttct tgttcttttc cgggaggata
     2281 acggccttga tccccgccct cttggccgcg agcacctttt ccttcactcc tccgatgggc
     2341 atcaccttgc cgcggagcgt tatctcgccg gtcatggcga ggtcatgcct ggccggtttg
     2401 ccggtcagca tggaaacgag ggcagtggtc atggtcaccc ccgccgacgg cccgtccttc
     2461 gggatcgcgc ctgccggcac gtggatatgg atgtccgttt tttcgaagaa gtccctgttg
     2521 atcccgaagg tctccgcctg ggaccggaca taggaaagag cggcctgcgc cgattctttc
     2581 atcacgtcgc cgagctgccc cgtcagggtc aactgcttgt tccccggcat ctgcgtggcc
     2641 tcgacaaaga gaatgtcccc tcccgtcggt gtccaggcca gcccggtcac aacccctggg
     2701 cggtcgatca tgatcgccgt ctcggcgaaa aatctggggt tgccgagata atcgtgcagg
     2761 tctcccacct tgacggtgat cggcgtcggt ttgccttcgg ccacggcctt cgccaccttg
     2821 cggcagacat tcgcgatctc gcgctcgacg ttcctgacgc ccgcctcgcg cgtatactct
     2881 ttgatgatac cccggagggc gtcctcctct atcacgatct cctctggttt gagcgcattg
     2941 gccttgatct ccttggggat gagatacttc tgcgctatcc tcagtttttc atgctcggtg
     3001 tagccgggta gatggagcac ctccatgcgg tccagcagag gcgggggaat cgtctcgagc
     3061 tggttcgccg ttgtgatgaa catcaccttc gagaggtcga agggaacatc tagataattg
     3121 tcgcggaagt ccttgttctg ctccggatcg agcacttcg
//
LOCUS       DASSLD010000314         3481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_252950,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000314 DASSLD010000000
VERSION     DASSLD010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252950"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1020
                     /locus_tag="VFK23_05655"
     CDS             169..1020
                     /locus_tag="VFK23_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7318603.1"
                     /translation="MSIRDPGNRFVMPALILLSLVTVYPVSSVLYLSLHRRLLIFNIS
                     KFVGADNYLHLLSDDRFWNAFRNTVFFTAASVSLELLLGLALALILNRNFRFRGAVTA
                     IVLVPWAIPTVVSARMWEWMYNTDFGILNHLLGVKINWLGSPFWALNAAVFMDIWKTT
                     PFVVILLLAGLKVIPRELYQSARIDGADSWNIFRRITLPLLMPVVLVVLIFRTLDAFR
                     VFDAVYVLTGGGPANTTETLSIYAYKVLFQTLQFGYGSTLSMVVFLCVGLLSVLYIRL
                     LRGEVKS"
     gene            1017..1829
                     /locus_tag="VFK23_05660"
     CDS             1017..1829
                     /locus_tag="VFK23_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7318604.1"
                     /translation="MKRAWFLLTLFVAALWCLGPLLWQALTSLKPDAELTRLPPMLPQ
                     APTFEHYRSIFTGVPFLRIIANSAFVASCSTALSIVIGSLAAFGLAKLNVRYKGFILI
                     FILSASMFPPIATVSPLFIIIRALHLRDTWWGLIVTYTTFTLPLSIWIMTNFFREIPD
                     EIYLASRVDGCSPFQSFVRIMLPLAAPGIATAAILVFIFAWNEFLFALTFTATPASRT
                     IPVAIALFPGLHEMPWGDIAAASIVVTIPLIVLVLMFQRRIIEGLTAGAVKG"
     gene            2003..3070
                     /locus_tag="VFK23_05665"
     CDS             2003..3070
                     /locus_tag="VFK23_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7318605.1"
                     /translation="MAEIRFENVTKRFGDVEVVSGLSLTIHDGEFFTFVGPSGCGKST
                     ILNMIAGLEDVTGGSLLFDDRVVNGLSPKDRDVAMVFQSYALYPHMSVYENIAFPLRM
                     KKEKNEVIDKEVRRVALLLGIAGTLPRKPRELSGGQRQRVALARAIVRRPKVFLMDEP
                     LSNLDARLRMETRAELKKLHRDLGITTVYVTHDQAEALGLSDRICVIKGGAVQQVGTP
                     LDVYAKPANMFVGGFIGSPPMNFLPCSLRSNAPLEIDCLGLLIRPHTEHAPAGGELLA
                     GVRPDDVSVTSKKPAEGAEVTVSVVEPAGSFTWVDVARGDVKLRGFSRQAGNLIPDGR
                     AFVSFPPEAVIVFDASTGSRL"
     gene            3142..>3481
                     /locus_tag="VFK23_05670"
     CDS             3142..>3481
                     /locus_tag="VFK23_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018946295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HET7318606.1"
                     /translation="MKKMLERLYDYFARDLWAVGRPSPRGLHALLIGALRIVTVVVRD
                     VIYGEITLRATGLVYSTLLSLVPLLAVSFSVLKAFGVHNALEPFLLNLLTPLGSTAAD
                     LAQRIVGFVDN"
BASE COUNT          639 a         1037 c         1012 g          793 t
ORIGIN      
        1 gtatcagata ttcatgaaga cccgtccgcg tcccgtgaca ccctactatc cgatgatcac
       61 gcagattatg cagccggagt tcagcgccgc catctccggg atcaagacac ccgaagacgc
      121 gctcggttcg gcccagaaac agatcgagta tgtcctggga ggagaaagat gagcatcagg
      181 gaccccggaa acaggtttgt catgccggcc cttatcctcc tgtccctggt cacggtttat
      241 ccggtgtcct ctgttctgta tctgagcctt caccggaggc ttttgatatt caatatttca
      301 aagtttgtgg gagcggacaa ttatctgcac ctgctttccg acgaccggtt ctggaacgcc
      361 ttccgaaata cggttttctt cacggcggca tcggtttctc tggagctgct tcttggtctg
      421 gctttggcat tgattctaaa ccgaaacttc aggttcaggg gggccgtcac ggcaatcgtc
      481 cttgtaccct gggccatccc tacggttgtt tcggcgcgga tgtgggaatg gatgtacaac
      541 accgacttcg gcatcctgaa ccacctcctg ggcgtgaaga tcaactggct tgggagccct
      601 ttctgggcgc tgaacgcggc cgtgttcatg gacatctgga agacgacacc cttcgtcgtc
      661 atcctgcttc ttgccgggct caaggtgatt cctcgggagc tctaccagtc cgcgcgcatc
      721 gacggggccg attcctggaa catattccgg aggataaccc tgcctttact catgcccgtg
      781 gtcctggtgg tcttgatctt tcggaccctt gacgccttca gggtcttcga cgccgtatat
      841 gtcctgaccg ggggcgggcc ggccaatacc accgagacgc tttccattta tgcctataag
      901 gtcctgtttc agaccctcca gttcggatac ggctccacgc tgtccatggt cgtctttctc
      961 tgcgtcggcc tcctgagcgt cctgtatatc cgcctcttgc gcggcgaggt gaagtcatga
     1021 aacgggcatg gtttctcctg acgctttttg tcgcggcgct ctggtgcctc ggtcccttgc
     1081 tctggcaggc ccttacgagc ctcaagcccg acgccgaact cacccggctg ccgcccatgc
     1141 tgcctcaggc gccgacgttc gaacattacc gctcaatctt taccggagtt cccttcctga
     1201 ggatcatcgc gaacagcgcc ttcgttgcgt cgtgctccac ggcgctttcg attgtcatcg
     1261 gatcgctcgc ggcgttcggg cttgcgaaac tgaatgtacg gtacaagggg ttcatcctga
     1321 tattcattct ttcagcctcc atgtttccgc cgatcgccac cgtgagcccg ctcttcatca
     1381 ttatccgtgc gctccacctt cgcgacacct ggtgggggct tatcgtcacc tatacgacct
     1441 tcacgctgcc gctctccatc tggatcatga ccaacttctt ccgggagata cccgacgaga
     1501 tctatctcgc gtcccgcgtt gacggatgca gcccctttca gtcctttgtc aggatcatgc
     1561 ttcccctggc ggcgccgggc atcgcgaccg ccgcgatcct tgttttcatt ttcgcctgga
     1621 acgagtttct cttcgccctc accttcacgg caacgcctgc ctcgcgcacg atacccgtgg
     1681 caatcgccct gttcccgggc cttcacgaga tgccgtgggg agacatcgcg gccgcgtcga
     1741 tcgtggtcac catcccgctc atcgtcctcg tgctcatgtt ccagcggagg atcatcgagg
     1801 gcttgacggc aggggcggtc aagggatgat accaatgcgg attgtggaat gcggaatgaa
     1861 gtatatcata ctacatcgtc attcccgcgg acgcgggaat ccattttgat atatcttcga
     1921 taagcggatt gataccccgc tggagcctgc ccctgcatga ttgaagcagg ggcgggtatg
     1981 acggcgtatt tagtaccaac ctatggcaga aatacgattc gaaaacgtga cgaagaggtt
     2041 cggcgacgtc gaggtcgtca gcgggctctc cctcacgatc catgacggcg agttcttcac
     2101 ctttgtgggg ccgagcgggt gcgggaagtc gacgatcttg aacatgatcg cgggactgga
     2161 ggacgtgacc ggcggcagtc ttcttttcga tgaccgggtg gtgaacggcc tttcccccaa
     2221 ggaccgcgat gtggccatgg tcttccagag ctacgccctc tatccgcaca tgagcgtgta
     2281 tgagaacatc gccttcccgc tcaggatgaa aaaggaaaag aacgaggtca tcgataagga
     2341 ggtcaggcgc gtcgcgttgc ttctcggtat cgccggaacc ctcccgcgga aacccaggga
     2401 gctttccgga ggccagcgcc agcgcgtggc cctggcgagg gcgatcgtga gaaggcccaa
     2461 agtgtttctc atggacgaac ccctttcgaa cctggacgcg cgtctcagga tggaaacgcg
     2521 cgcggagctt aagaagctcc accgggatct cgggatcacc acggtatacg tgactcacga
     2581 ccaggcggag gccttgggcc tctcggacag gatctgcgtg attaagggag gagccgtcca
     2641 gcaggtgggt acgcccctgg acgtctatgc gaagccggca aacatgttcg tgggagggtt
     2701 cataggcagc ccgcccatga acttccttcc ttgttcgttg cggagcaatg cgcccctgga
     2761 gatcgactgt ctcggtctcc tgatcaggcc gcacactgag cacgctccgg ccggcgggga
     2821 actcctggcg ggcgtgagac ccgacgacgt gtcggtgacg tccaagaagc cggcggaggg
     2881 agcggaagtc accgtatcgg tcgtcgaacc cgcagggtct tttacgtggg tggacgtcgc
     2941 acggggcgat gtgaaactga gaggattctc acggcaagcc gggaacctga ttccggatgg
     3001 cagggctttc gtgtctttcc cccctgaagc cgtcattgtc tttgatgcct cgacagggag
     3061 caggctgtga cgaacgtttg aggccgtgga gcaatcgagc gctacccgag gtgatagaat
     3121 aaaaaaagag agctggtcat catgaaaaaa atgctggaac ggctgtacga ctattttgcg
     3181 cgcgacctgt gggcagtagg ccggccgtcg cccagagggc ttcatgccct gctgatcggg
     3241 gctctccgga tcgttaccgt cgttgtgagg gacgtcatat acggcgagat aacactgcgg
     3301 gccacaggcc tcgtgtactc gacgctcctg tcgctcgtgc ccctgctcgc ggtcagtttc
     3361 tcggtgctca aggccttcgg cgtgcataat gcgcttgagc cgttcctgct caatctcctg
     3421 accccgcttg ggtccacggc cgcggacctg gcgcagcgaa tcgtgggttt cgtcgacaac
     3481 a
//
LOCUS       DASSLD010000315         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_921197,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000315 DASSLD010000000
VERSION     DASSLD010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921197"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFK23_05675"
     CDS             <1..394
                     /locus_tag="VFK23_05675"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7318607.1"
                     /translation="NPLRHFFQDPEKIVGPFVREGMTVADIGCGMGYFTIPMARIVGA
                     KGTVFAVDIQERMLEFTDRRSKRAGVGDRVRTVRAAGDDIGIREPVDFVLAFWMVHEV
                     KDITRFFGQLSSVLKEGGRVLYVEPLSM"
     gene            683..1321
                     /locus_tag="VFK23_05680"
     CDS             683..1321
                     /locus_tag="VFK23_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020059216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HET7318608.1"
                     /translation="MIIKALNIGLPKKEIFHGKEFITGMCKKPVSGPLLLTKLGFEGD
                     GVGDHKNHGGADKAVCVYSLDHYGYWKGILGTPMPEAAFGENFSVTGLQEGEIQIGDI
                     FRAGSALVQVSQPRQPCSTLAARYGRSDFAKLVSDSDRTGFYLKVLEEGKVKAGDILD
                     LVEKDSRQVSISFANHVWYHDRKNREGIEKVLSVPALSEAWRKSFLELKEKA"
     gene            1450..1677
                     /locus_tag="VFK23_05685"
     CDS             1450..1677
                     /locus_tag="VFK23_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318609.1"
                     /translation="MKKNILFIAAAAAILLLLFVLSQGKKAPLIPVDDFHKIITTNEA
                     CAECHGPGKRAPLKQTHPPKEQCLICHKVKR"
     gene            2273..>2741
                     /locus_tag="VFK23_05690"
     CDS             2273..>2741
                     /locus_tag="VFK23_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HET7318610.1"
                     /translation="MKSAASAPATASGKGLNLSENCIKVLEKRYLKRNEDGTVVETAV
                     DMFTRVARTIAGADRAYGASDADVERTATEFYRLMTSLEFLPNSPTLMNAGRELGQLS
                     ACFVLPVGDSMDEIFESVKHAALIHNSGGGTGFSFSRIRPKNDVVKSTKGVSSG"
BASE COUNT          625 a          752 c          760 g          604 t
ORIGIN      
        1 taaccccctc cgccattttt tccaggatcc ggaaaagatc gtcggcccct ttgtgcggga
       61 agggatgacc gttgccgaca tcgggtgcgg catggggtac ttcacgattc ccatggcgag
      121 gattgtggga gcgaagggaa cggtttttgc cgtggatatt caggaaagga tgctcgaatt
      181 cacggatcgg cggtcgaaga gggcgggcgt gggtgaccgc gtccgcaccg ttcgcgcagc
      241 aggggacgat atcgggatca gggagcccgt tgatttcgtc ctggccttct ggatggtcca
      301 cgaggtcaag gacattacgc ggttcttcgg acagctctcg tccgtactga aagaaggcgg
      361 aagggtcctg tatgtcgagc cgctttccat gtgacggagc gccggttccg ggagatactc
      421 ggtttcgctc aaactgcagg catccggacg aacgaggcgc cccacattgc cttcagccga
      481 gcggtgatct tggagaaaaa aggttagcca gccgctggcg cctttttctt ttgatgtcat
      541 tccgggcttg gcccggaatc cattttatca attggattcc cgctggagcc tgccccagca
      601 agattttagc aggggcggga atgacaggat atggggcaat acattgcctc ataacgatat
      661 cacgtagctc agaaagggaa aaatgatcat caaagccctc aacatcggtc ttccgaaaaa
      721 ggaaatcttc cacggcaagg aattcatcac cggcatgtgc aaaaagccgg ttagcggccc
      781 cctcctgctg accaaattgg ggttcgaggg cgacggcgtg ggagaccata agaaccacgg
      841 cggggcggac aaggccgtct gcgtctacag tctcgaccac tatggttatt ggaaggggat
      901 cctcgggacc ccgatgccgg aagcagcctt cggagaaaac ttttccgtta ccggccttca
      961 agaaggtgag atccaaatcg gcgacatttt cagggccggc tccgccctgg tgcaggtcag
     1021 ccagccgcgc cagccgtgtt cgacgcttgc cgcgcggtat ggcaggagtg attttgcaaa
     1081 gctcgtatcc gattcggacc gtacgggttt ctacctcaag gtgcttgaag aggggaaggt
     1141 aaaggccgga gacatcctcg accttgtcga gaaggacagc cgccaggtct cgatttcctt
     1201 tgccaatcat gtctggtatc atgaccggaa aaaccgcgaa ggcatcgaga aggtcctgtc
     1261 cgtgcccgcc ctttcggagg catggcggaa gtcgttcttg gagttgaagg aaaaggcgtg
     1321 acaatagtaa ccacaggcga ctttgtcgcg cacagtaaaa cgatattcag gatatttatt
     1381 atctaaccag tgttcatctg tgcgttctgc gaagcagaac ccgtggttac actctgccgt
     1441 gagggtttta tgaaaaagaa catactcttt atcgctgctg ccgctgcgat cctccttttg
     1501 ttgttcgtgc tctcccaagg caagaaagcg cctttaatcc ccgtcgatga tttccataag
     1561 atcataacca cgaacgaggc ctgcgcagaa tgccacggcc cgggaaaacg agcgcccctc
     1621 aaacagaccc accctccaaa ggagcagtgc ctcatttgtc ataaggtgaa gcgataaaag
     1681 gattgcgccg cggtgaactg tttcgccttc cgcccgatcc cgggcccggc tatccggacc
     1741 ccttcactcc tcattgcaca tcctctccgc atctggagcg gggtttaaaa acctcatttt
     1801 ttttaaactg attccgtact gatttcatgc ccctggaata gacctccttt cttagaatcc
     1861 tcgcagaatc tgctaaatgt ggtggtagaa tcggcttgac aacacaacat attggggtat
     1921 atacttccca acagttttta catctgcatg accgtttcca ctggtatttt gctttccccg
     1981 gcttcttgca agaaacctca taaaaggctg catccaagag aaacaccaca ggcagggcca
     2041 tatgggaata tcggtaaaat actgttttag cccgctgaga gccgacgcca cccgaagcga
     2101 gaagtaccct acagtcatga tcagacgatc ggcagcggtt ttaacgggct tcattcatct
     2161 ggttagtcaa tcggtcaagt tgtaatatga acgccggggc ggtcatgcat gccgccccgg
     2221 ctttgatttc aattcataac ttttatcagt atcatcaggg agggaaatct ttatgaagag
     2281 tgcggcgtca gctccagcaa cggcttccgg caagggactc aatctatccg agaattgcat
     2341 caaggtcctc gaaaaacgct acctcaaacg gaacgaagac gggaccgtcg tcgaaacagc
     2401 ggtcgacatg ttcacgcgcg tggcgcggac gatcgccggg gccgaccggg cctacggagc
     2461 gtccgacgcc gacgtggagc ggaccgcgac cgagttctac cgcctcatga cctccctcga
     2521 attcctgccg aacagcccca cgctcatgaa cgccggccgt gaactcgggc agttgtcggc
     2581 ctgcttcgtc ctgcccgtgg gcgactccat ggacgagata ttcgagtccg tgaagcacgc
     2641 ggccctgatc cataactccg gaggcggcac gggcttctcc ttttcgcgca tccgtccgaa
     2701 gaacgacgtg gtgaagtcca cgaagggcgt ttcgtccggt c
//
LOCUS       DASSLD010000316         3814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1247556, whole genome shotgun sequence.
ACCESSION   DASSLD010000316 DASSLD010000000
VERSION     DASSLD010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247556"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /gene="trpS"
                     /locus_tag="VFK23_05695"
     CDS             <1..948
                     /gene="trpS"
                     /locus_tag="VFK23_05695"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET7318611.1"
                     /translation="SGLLHLGNLMGALDNWRKLQDQYECFYFIADWHALTTNYADTSN
                     IRTYVREIVIDWLAAGLDPEKAVLFQQSHVPEHAVLHVLLSMCTPLPWLERVPTYKEK
                     MDQIQDREMNTYGFLGYPVLQAGDILIYKADFVPVGTDQLPHLELSREICRRFNQFYG
                     DLFPEPQPLLTEYPKVPGTDGRKMSKSYNNCLYLSDSPDAITKKVMQMVTDPARVRRQ
                     DPGNPDVCPLFTLDKMFAPKEWCDYVNGECRRAGIGCVDDKKELLKHLLAFLAPIHEK
                     RIEYVAQPDKIAEIIAEGSRKAHLIAAKTLAEVNEAMKL"
     gene            1143..1967
                     /locus_tag="VFK23_05700"
     CDS             1143..1967
                     /locus_tag="VFK23_05700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7318612.1"
                     /translation="MKEPDSSFYKESALYENRSQETQNNTPSHDWRYETCLSSLGSQP
                     GRSLLDVGCGDGTFLVRARDQGFKVFGIDFDDRAVSIAKKIRKLEQVECGRWDSISRM
                     SGWEQFDVITMFDVLEHVAAPGDLAATAYALLKPNGTMAISVPRFDRYPRMFDIEYDF
                     PPHHFTLWTSKAMIVLLEKTGCEDIRIVEKPLEAKDVLEHIKWRVKRLVRRLRRGSEE
                     PSGRPDPRTAPAGVIKTALKIFEKPLLLMLGLAVLFLRAIHLARGHTLLVLAKKKA"
     gene            2181..3167
                     /locus_tag="VFK23_05705"
     CDS             2181..3167
                     /locus_tag="VFK23_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET7318613.1"
                     /translation="MTITTIQDVLKEYKSDMERMEHRIHESLGTDVPLIQQVSKYILG
                     AGGKRFRPLLHLLSARLCGFRGHDAEYVLGSVVEYIHTASLLHDDVVDEAKLRRGRDS
                     ANSLWGNQASILVGDYLYSKALYHAVRLHNQRVMDVLSETTTTMSEGEVLQLMQIQNS
                     EITEADYYRLVECKTGVLISASCRLGAIISKAPLSQEDALTAYGKKLGLAFQITDDTL
                     DYAADQRRLGKALGKDLAEGKVTLPLIYLMRKADKEERGRLREILQVDAMADSDLTYT
                     LGLMEKHGTVDESLAVAQALSNEAKAALALFPDTPPRQALMALADYVVQREM"
     gene            3167..3679
                     /gene="amrA"
                     /locus_tag="VFK23_05710"
     CDS             3167..3679
                     /gene="amrA"
                     /locus_tag="VFK23_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HET7318614.1"
                     /translation="MHPLVRLAKDTVELYVRKGKIYEVREDELTPDLKGRAGVFVCLK
                     IFGHLRGCIGTFQPTEPTIAHETVKNAISAATCDPRFACVHADELDSIGYTVDVLTPP
                     EKVATKDELDPRRYGVIVHAGGRRGLLLPDLEGVDSADHQIGIAMQKAGIPPGTPVEL
                     FRFEVKRYGE"
BASE COUNT          848 a         1176 c         1090 g          700 t
ORIGIN      
        1 agcgggcttt tgcatctcgg gaacctcatg ggcgccctcg acaactggag gaaactgcag
       61 gaccagtacg agtgcttcta cttcatcgcc gactggcacg ccctcacgac gaactacgcc
      121 gacacgtcga acatccgcac ctacgtcagg gagattgtca tcgactggct ggccgccggg
      181 ctcgatcccg aaaaggccgt gctcttccag cagtcccatg tgccggagca cgccgtgctc
      241 cacgtgctcc tgtccatgtg tacgccgctc ccctggctgg agcgcgtgcc cacgtataag
      301 gaaaagatgg accagatcca ggacagggag atgaacacct atggctttct gggctatccc
      361 gtgctccagg cgggggacat cctgatctac aaggcggact tcgtgccggt cggtacggac
      421 cagctccccc acctggaact gtcccgcgag atctgccgcc gcttcaacca gttctacgga
      481 gatctgttcc ccgagcccca gccgctcttg accgaatacc ccaaggtccc gggcacggac
      541 ggccgcaaga tgagcaagag ctacaacaac tgcctctacc tgtcggacag ccccgatgcg
      601 atcacgaaga aggtgatgca gatggtcacg gaccccgccc gcgtcaggag gcaggacccc
      661 ggcaatcccg acgtctgccc gctcttcacg ctcgacaaaa tgttcgcgcc gaaggagtgg
      721 tgcgattatg tgaacgggga atgccggcgc gcgggcatcg gctgcgtaga cgacaaaaaa
      781 gaacttctga agcatctcct cgccttcctc gcgcccattc atgaaaagcg catagagtac
      841 gtcgcccagc cggacaagat cgccgagatc attgccgaag gctcccgcaa ggcccacctc
      901 attgctgcga agacgctcgc tgaagtgaac gaggcgatga agttgtaggg acggagccgc
      961 ggcaccggcc gcggaaacgt acgggaagaa cgttagcgag tatctacgta cttataccca
     1021 aagaggcttt catgaagacg tgccttttat gccggaacgc ttcctccgtc cgctctctcc
     1081 ggatgcacgg cggctatagt attctgtatt gttctgactg tcggctcagg ttttccgacc
     1141 ccatgaaaga acccgactcg tcgttttaca aggaaagcgc tctgtatgaa aaccggtctc
     1201 aggaaacaca gaacaacacg ccgtcccatg actggcgcta cgagacctgt ctctcctccc
     1261 ttgggtcaca gccgggccgg tcgttgctgg atgtcgggtg cggcgacgga acgttcctgg
     1321 tccgggcgcg ggaccaaggc ttcaaggtat tcgggataga cttcgatgat cgggcggtga
     1381 gcatcgcaaa aaaaattcgt aagctggaac aggttgaatg cggcagatgg gacagtatat
     1441 cacgcatgag cggctgggaa cagttcgacg taattacgat gttcgacgta ctggagcatg
     1501 tggcggctcc gggagacctg gcggcaactg cgtacgccct gctcaagcca aacggaacga
     1561 tggcaatctc cgtgccgcga tttgaccggt atccccgaat gttcgatatt gagtatgatt
     1621 ttcccccgca ccattttact ctgtggactt caaaagcgat gatcgtcttg ctggaaaaga
     1681 ccggctgcga agacatcagg attgttgaaa agcccctcga ggccaaggat gtcttggaac
     1741 atatcaagtg gcgggtaaag aggctggtaa gacggcttcg gcgcggcagc gaagagcctt
     1801 cgggacgacc cgatccccga acagctcccg ccggggtgat caagaccgct ctgaagatat
     1861 ttgaaaagcc cttattgctc atgctggggc tggcggtcct gtttctccgt gccattcatc
     1921 ttgcgcgcgg gcataccctg ctcgtccttg ctaagaagaa agcctgagcg gggccggacc
     1981 acgatgagcg tgccggtgtg tgacactctg tccaatgccg ccacgctgct cagggtcctt
     2041 cccgtcacgt cccgctggag ccggatatcc ggagcggtcc gcacccgtta gctttttctc
     2101 accctgcgcg ccgtctataa aatccttgac aataaaggac ttctccccta ttatttcgtt
     2161 agtctgccgg ggaaaaaacg atgacgataa cgacgatcca ggacgtactc aaggaatata
     2221 agtccgacat ggaacgcatg gagcacagga tccatgagag cctgggcacc gatgtcccgc
     2281 ttatccagca ggtcagtaaa tatatcctcg gagcgggcgg caagcggttc cgccccctgc
     2341 tgcacctcct ctcggccagg ctttgcgggt tccgcggaca cgacgccgaa tacgtcctgg
     2401 gcagcgtggt cgaatacatc cacacggctt cgcttctcca cgacgacgtc gtcgatgaag
     2461 ccaaactcag gcgcggccgc gactccgcca attcgctctg gggcaaccag gcgagcatcc
     2521 tggtcggtga ctacctctac tcaaaagccc tctatcacgc cgtccgtctc cacaaccagc
     2581 gcgtcatgga cgtcctttcc gaaaccacga cgaccatgtc cgaaggcgaa gtgctgcagc
     2641 tcatgcagat ccagaactcc gaaatcaccg aggcggacta ttaccggctc gtcgaatgca
     2701 aaaccggcgt gctgatctcc gcgtcgtgcc ggctcggcgc cattatcagc aaggccccgc
     2761 tctcgcagga ggacgccctg accgcctacg gcaagaaact gggtctcgcg ttccagatca
     2821 ccgacgacac gctcgactac gcggcggacc aaaggcgtct cggcaaggca ctcggcaagg
     2881 accttgcgga gggcaaggtc acccttcccc tcatttacct catgcgcaag gcggataaag
     2941 aagagcgcgg ccgcctccgg gaaatactcc aggttgacgc gatggctgac agcgatctca
     3001 cctataccct gggacttatg gaaaaacacg ggaccgtgga cgagtcgctc gccgtggccc
     3061 aggcgctgtc caacgaggcc aaggccgccc tcgcgctttt tcccgatacg ccgccccgcc
     3121 aggcgctcat ggcgctcgcc gactacgtcg tacagcggga gatgtgatgc atcccctcgt
     3181 caggcttgca aaagacacgg tggagctcta cgtcaggaaa ggcaagatct atgaagtccg
     3241 ggaggacgag ctcacccccg atctgaaagg gcgggccggc gtgttcgtct gcctcaagat
     3301 attcgggcac ctgcgcggat gcatcgggac gttccagccc actgagccca ccatcgccca
     3361 cgagaccgtg aagaacgcga tcagcgccgc cacgtgcgac ccccgttttg cctgcgtcca
     3421 cgccgacgaa ctggactcca tcggatacac ggtggatgtg ctgacacccc ccgagaaggt
     3481 agccacaaaa gacgaactcg atccccggcg ctacggcgtc attgtgcatg cgggaggaag
     3541 acggggcctg ctgctgccgg acctcgaagg cgtcgactcc gcggaccacc agatcggcat
     3601 agccatgcag aaggcgggaa tacctcccgg gacgccggtc gagcttttcc ggtttgaagt
     3661 aaaacggtat ggagaataag ctgccggcag ccggattccc gcttttagcg aatcccgacc
     3721 gctggcagtc gcatcccaac aaatctacat aaggaggtat ggcaggacca atggacagac
     3781 cggaaagaac agaaaaagac gatacacaga agtt
//
LOCUS       DASSLD010000317         3526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_314035,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000317 DASSLD010000000
VERSION     DASSLD010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314035"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..2283
                     /gene="glyS"
                     /locus_tag="VFK23_05715"
     CDS             205..2283
                     /gene="glyS"
                     /locus_tag="VFK23_05715"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET7318615.1"
                     /translation="MGKEFLLEIGTEEIPSRFINPAIEKMKELFAALLVSGRVASRGD
                     LRAYGTPRRLVLHCTDMDERQSDISREVVGPPKKVAFDDAGKPTRAAAVFAEKNGIPV
                     QALSFKSTEKGEYVVARIDEKGGSTAEWLREALPGFILSIPFPKSMRWMDKDIRFARP
                     IHWLLALWGGALVDFPLNGLKSGNLSRGHRFMSPGAFLVKDFKSYYSQLEPNFVVIDP
                     AARRSRIEKQTAELAANRNGAVLDDPGLVDEVANLVEFPVAVMGDFNKDYLRVPKEVL
                     ITAMREHQRYFSVVDANGNLLPYFITISNTRAEDMDIVRAGNERVLAARLSDAAYFFD
                     HDVKVPFADRVEGLKKVTYQEKLGTLYDKTVRVKTLAGYLAGLLKADRKASVRAAELC
                     KADLLTGVVGEFPKLQGVMGRHYALNSGEEPSVADAILEHYLPRFAGDTLPRSLEGVC
                     VGIADRIDTITGCFSAGLIPSGSEDPYGLRRQSVAILAMLLDRGLRLSLSGLVAEACR
                     GYGIKNADKVAAETLDFLRQRLSGMLASEGLRSDIVDAALSVGFDDPVIGREKVRALD
                     ALRASEDYQPLVTALKRAGNILPKGFSGGVDESLLAQEAEKALYAAFMMIKDRVVEKT
                     AKLDFRGALADIASLRKPVDAFFDTVMVMDKDAAIKANRLALLAGITGLFSQIADFSR
                     LVLGTEESKA"
     gene            2411..>3526
                     /locus_tag="VFK23_05720"
     CDS             2411..>3526
                     /locus_tag="VFK23_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET7318616.1"
                     /translation="MANGKKYIYFFGSGKADGTGAMKDLLGGKGAGLAEMTNAGVPVP
                     PGFTITTEVCNVFYDLGKRVPEGLDEDMRGHMQKVEEALGGKFKFGDPDRPLLVSVRS
                     GSKFSMPGMMDTVLNLGLNDKTINGLIRMSANERFAWDAYRRFIQMFGNVVMGIEKDQ
                     FEHIIHDVKKKKKVSLDIELTAEDLKGLVAKFKAKVKQITKRDFPQDPWEQLVMARDA
                     VFGSWNNPRAITYRKLNDIPGNLGTAVNVQSMVFGNMGETSATGVGFTRNPSTGAKEF
                     YGEYLTNAQGEDVVAGIRTPHPILDLKKEMPKVYRQLRDITSRLERHYRDVQDFEFTV
                     QEGTLYMLQTRTGKRTAHAAVKIAVDMVKEGFISKDEA"
BASE COUNT          750 a         1043 c         1097 g          636 t
ORIGIN      
        1 atgctgaaaa aataggtaaa gcgtccgggg gcaagggtcg aggaaaaact caatccgtct
       61 cgcgcaatgg ccaagaaaag ggccacactg aggcgcagag tccgcaacga agtacagatg
      121 ttttggctta ttaaaataag aatataacga ttccctttgc gccctccgcg tccttgcgtg
      181 agaatatttt cttaggagtt ttttttggga aaagaatttc tactagagat cggcactgaa
      241 gagatcccgt cgcgcttcat caatccggcg atagagaaga tgaaggagct ttttgccgcg
      301 ctcctcgtct cggggcgcgt tgcctcgcgc ggggatctca gggcctacgg gacgccgaga
      361 cgcctggtcc tccattgcac ggacatggac gagcgccagt ctgatatctc cagggaagtg
      421 gtcgggccgc cgaagaaggt ggccttcgat gacgcgggca agcccaccag ggcggcagcg
      481 gtcttcgccg agaagaacgg gatcccggtc caggcgcttt cattcaagtc cacggagaag
      541 ggagagtatg tcgtcgcccg gatcgacgaa aagggcggca gcacggctga atggctgaga
      601 gaggcgcttc ccggctttat cctgtccatt ccgttcccca agtccatgcg ctggatggac
      661 aaggacatcc ggtttgcccg cccgatccac tggctgctcg cactctgggg cggcgccctc
      721 gtcgatttcc ccttgaacgg gttgaagagc ggcaacctct cgcgcggcca ccggttcatg
      781 agccccggcg cttttctcgt gaaggacttc aagtcctatt actcccagct ggagccgaac
      841 ttcgtggtca tcgaccccgc ggcgcgaaga tcgcgcattg agaagcagac cgcggagctt
      901 gccgcgaacc ggaacggcgc ggtgctcgac gaccccgggc ttgtggacga agtggcaaac
      961 ctcgtggagt ttccggtcgc ggtcatggga gacttcaaca aggattacct ccgggtgccg
     1021 aaagaggtgc tgatcacggc gatgcgcgaa catcagcgct acttcagcgt cgtggacgcg
     1081 aacgggaacc tgctccccta tttcatcacg atcagcaaca cccgggccga agacatggat
     1141 atcgtcaggg cgggcaacga gcgcgtgctc gcggcgcggc tttccgacgc ggcatacttc
     1201 ttcgaccacg acgtgaaggt gccgttcgcc gaccgcgtcg aagggctcaa gaaagtgacg
     1261 taccaggaaa aactgggcac actttacgac aagaccgtgc gcgtgaagac gctggccgga
     1321 tatctggccg gtctcctcaa ggcggacaga aaggcgtcgg tgcgggcggc cgaattgtgc
     1381 aaggctgacc tcttgacggg cgtcgtcggc gagttcccca aactccaggg cgtcatgggc
     1441 aggcactatg cgctcaactc cggcgaggag ccgtccgtcg cggacgccat cctggagcac
     1501 tacctgcccc gctttgccgg cgacacgctc ccccgctcac tcgaaggagt ctgcgtgggg
     1561 atcgccgacc gcatcgatac gatcacgggg tgcttctccg cggggctcat cccgagcggg
     1621 tccgaagacc cttacggcct caggagacag tcggtggcca tccttgccat gctgcttgac
     1681 cggggcctcc ggctttcgct ttcgggcctc gtcgccgagg catgcagggg atacggcatc
     1741 aagaacgcgg acaaagtcgc ggcggagacc ctcgactttc tgcgtcagcg gctgtccggc
     1801 atgctggcgt ccgaggggct ccgttccgac atcgtggacg ccgcgctgtc cgtcgggttc
     1861 gacgacccgg tcatcggacg cgagaaggta agagcgctcg acgcgctgcg cgcctccgag
     1921 gattaccagc cgctggtcac ggccttgaag cgggcgggca acatcctgcc gaagggcttt
     1981 tccggcggtg tcgatgagag cctgctcgcc caggaagctg aaaaggcgct gtacgccgct
     2041 ttcatgatga tcaaggaccg cgtcgtcgaa aagaccgcga agcttgattt ccgcggcgcc
     2101 ctcgcggaca tcgcgtcgct tcggaagccc gtcgatgcat ttttcgacac agtcatggtc
     2161 atggacaagg acgccgcgat caaggcaaac cgtctggcgc tccttgccgg aatcaccggg
     2221 cttttttcgc agatcgcgga cttctcgcgg ctcgtgctcg gcacggaaga atcgaaagcc
     2281 tgattgaacc gcgaatcaac gcaaattatc gcgaataaag ccacagggca aagaatgaaa
     2341 attcgcgtca gttcgcgttt attgcgcagt taaaaaaaga ctctaaactt tttccaggga
     2401 gggttgtcac atggctaacg gaaaaaaata tatctacttc tttggcagcg gaaaggccga
     2461 cgggacaggg gcgatgaagg acctgctcgg cggcaagggc gcgggcctcg ccgagatgac
     2521 gaatgcaggc gttccggtcc cgccggggtt caccatcacg accgaggtct gcaacgtctt
     2581 ctacgacctc ggaaagcgcg tccccgaagg cctcgacgaa gacatgcgcg gccacatgca
     2641 gaaggtcgaa gaagcgctgg gcggcaagtt caagttcggc gacccggacc ggccgcttct
     2701 cgtttccgtg cgttcgggct ccaagttctc catgccgggc atgatggaca cggtcctgaa
     2761 cctgggcttg aacgacaaga cgataaacgg gctcatccgc atgtccgcca acgagcgctt
     2821 cgcctgggac gcctaccgcc ggttcatcca gatgttcggc aacgtggtga tggggatcga
     2881 aaaggaccag ttcgagcaca tcatccacga cgtgaaaaag aagaagaagg tgagcctcga
     2941 catcgagctc accgccgagg acctcaaggg gcttgtcgcc aagttcaagg cgaaggtgaa
     3001 gcagataacg aagcgcgatt tcccccagga cccctgggag cagcttgtca tggccaggga
     3061 cgcggtcttc ggctcctgga acaatccccg cgccatcacc taccggaagc tgaacgatat
     3121 tccgggcaac ctgggcacag cggtgaacgt gcagtccatg gtcttcggca acatgggtga
     3181 gacctcggcc acgggtgtgg gcttcacacg taatccctcg acgggcgcga aggagttcta
     3241 tggcgagtac ctgacgaacg cccagggcga ggacgtggtg gccggcatcc gcacgccgca
     3301 tcccattctg gacctcaaaa aggaaatgcc caaggtgtac cgccagctcc gcgacatcac
     3361 gagcaggctt gagcggcatt accgcgacgt gcaggatttc gagttcacgg tccaggaagg
     3421 cacgctctat atgctccaga cccgtacggg caagcgtacc gcccacgcgg cggtcaagat
     3481 cgccgtcgat atggtgaaag aagggtttat ctccaaggac gaggcc
//
LOCUS       DASSLD010000318         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_99422,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000318 DASSLD010000000
VERSION     DASSLD010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99422"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VFK23_05725"
     CDS             <1..1066
                     /locus_tag="VFK23_05725"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET7318617.1"
                     /translation="LKDMRERMGRIVVAYSKDGSPVTTEDLGVAGAMTAWMVEALNPN
                     LLQTIEGQPVFVHAGPFANIAIGQSSIIADRVGLKLGDYLVTESGFAADIGFEKFWNL
                     KCRMSGLKPHAAVVVATIRALKCHGGAPIPVPGKPMPAEYKGENVGWVEKGCENLVHH
                     IKTVKKAGINPVVCINAFYTDTDNEIKTVRRLAEAAGARVALSRHWEKGGDGALEFAD
                     AVIDACNEKNDFKLLYELDTPLRKRIELIAKEVYGADGVDYTPEAEAKAKKMEADPEI
                     AKLGTCMVKTHLSLTDNPNLKGVPKGWRLRIRDILTYKGAGFVVPVAGTITLMPGTSS
                     DPAYRRVDVDVETGKVKGVF"
     gene            complement(1169..1507)
                     /locus_tag="VFK23_05730"
     CDS             complement(1169..1507)
                     /locus_tag="VFK23_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318618.1"
                     /translation="MTTKVFVENLPCDITEERLKDVFMQVGAVGAVKIQKDLLTLQAR
                     CSGTVEMLLDVDAYRAVNCFNGARFRDRKIHVEEAKPLYEKARELLRDRGRLLAQYSA
                     SLKKQLPKWH"
     gene            complement(1535..1798)
                     /locus_tag="VFK23_05735"
     CDS             complement(1535..1798)
                     /locus_tag="VFK23_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET7318619.1"
                     /translation="MATKIHVENLPCDISEERLRDIFAQIGSVESVKLETDLITRRPK
                     CAGYVEMSLDVDAFRAVNCFDGATFKDRKIHVEEAKPFYERAK"
     gene            complement(1977..>2777)
                     /locus_tag="VFK23_05740"
     CDS             complement(1977..>2777)
                     /locus_tag="VFK23_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318620.1"
                     /translation="PAPAVPEDAERSMARGIARFQRAADSAGYKKAISEFEAAANAAP
                     WLAAAYYNLGMAQEKAGLYSEAIRNLKYYLLSAPGAKNAREVKNKIYTLEVDAEDLQA
                     GKNDQAPPAAPSADSGSIRPPRITGRASMEIEPAEKQLTIIKMLPPEKKPKMPSFIGA
                     WYFKDFLRGEELTIEAFEISKNASGDLILTPPKRAAESYASVTQLEISDRNLKLQLLW
                     KMRSIVGYWKTETYELTLSEDGKTLTGSHTQKSVGGRTIEMDRVLFRQ"
BASE COUNT          530 a          859 c          801 g          587 t
ORIGIN      
        1 cctcaaggac atgcgcgagc gcatgggccg gatcgtcgtg gcctacagca aggacggatc
       61 gccggtcacg accgaggacc tgggagttgc cggcgccatg acggcgtgga tggtcgaggc
      121 cctgaacccg aacctgctgc agaccatcga gggtcagccc gtgtttgtgc acgccgggcc
      181 cttcgccaac atcgccatcg gccagtcctc gatcatcgcc gaccgagtgg gcttgaaact
      241 cggtgactac ctcgtgaccg agtcgggctt cgcagcagac atcgggttcg aaaagttctg
      301 gaacctgaag tgccgcatgt cgggcttgaa gccccatgcg gccgtcgtgg tcgccacgat
      361 ccgagcgctc aagtgccacg gcggcgcgcc catcccggtg cccggcaagc ccatgcctgc
      421 cgagtacaag ggcgagaacg tgggctgggt cgagaagggc tgcgagaacc tcgtccatca
      481 catcaagacc gtgaagaagg cgggcatcaa ccccgtggtc tgcataaacg ccttctacac
      541 cgacacggac aacgagatca agaccgtgcg ccggcttgcc gaggcggcag gggcgcgcgt
      601 ggccctctcc cgccactggg agaagggggg cgacggcgcg ctcgagttcg ccgacgccgt
      661 gatcgacgcc tgcaacgaga agaacgactt caaactcctc tatgagctcg ataccccgct
      721 ccggaagcgc atcgagctga tcgcgaaaga ggtctacggc gccgacggcg tggactacac
      781 gcccgaggcg gaggccaaag cgaagaagat ggaggccgac cccgagatcg ccaagctcgg
      841 cacctgcatg gtgaagaccc acctctcgct cacggacaac ccgaacttga agggcgttcc
      901 caagggctgg aggctccgca tccgcgacat cctgacttac aagggcgcgg gcttcgtggt
      961 ccccgttgcc ggcaccatca cactcatgcc cggcacctcg tccgacccgg cctaccgcag
     1021 agtggacgtg gacgtggaga ccgggaaggt caaaggcgtg ttttaagaaa gaggtcccta
     1081 tccaggatgc aggataaaag aagcccaccc cggcgtgccg gggtgggctt tcgtatgaga
     1141 gatcggttct gaaaaatccg ggtttgtctc agtgccactt cggcagctgc tttttcaggc
     1201 tagcactgta ctgcgccagg agacggccgc ggtcccgcag gagttctctc gccttctcgt
     1261 acaaaggttt tgcctcttcc acatggatct tccggtccct gaacctcgcc ccgttgaaac
     1321 aattgaccgc ccggtaggcg tccacgtcaa gcaacatttc cacggttccg gaacacctgg
     1381 cctgcagtgt cagcaggtcc ttttgaattt ttaccgcccc gacggcgccg acctgcataa
     1441 agacgtcctt cagcctttcc tcggttatgt cgcacggcag gttttccacg aacacttttg
     1501 tcgtcatgtc atccgtgaac cggttcatga ggacctattt ggcccgttcg taaaaaggct
     1561 ttgcctcttc cacatggatc ttccggtctt tgaaagtcgc cccgtcaaaa cagttgaccg
     1621 cccggaaggc gtccacgtcg agggacatct ccacgtaccc cgcgcacttg gggcgccgcg
     1681 taatcaggtc ggtctccagc ttgaccgact cgaccgatcc gatctgggca aaaatgtccc
     1741 tgagcctctc ctcggaaatg tcgcacggta gattttccac atgaattttc gtagccatct
     1801 ccccgtctcc tttgttctct ccgcccgcga aacagcggcc agcacgcgct ggccttcatt
     1861 atagcaaaaa agcggtcggt ctgttgatct ccactctata agatgcgtcg ccagaggagg
     1921 ccccggctga aagcccgcaa tgcttccgcc ctgtatatca ggaagcacag cgccgtctac
     1981 tgccggaaga gcacccggtc catctcgatt gttcttcctc ccacgctttt ttgcgtgtgg
     2041 gagcccgtta acgtcttccc gtcttccgaa agggtgagct cataagtctc cgtcttccag
     2101 tatccgacga tcgatctcat tttccacagc agctgcagtt tgaggtttct gtcgctgatt
     2161 tctaactggg taacgctcgc ataactctcg gcggcgcgtt tcggcggcgt gagaatgagg
     2221 tcgccgctgg cgtttttgct tatttcaaac gcctcaatgg tgagttcttc cccgcgaagg
     2281 aagtctttga aataccaggc gccgataaaa cttggcattt taggtttttt ttcgggtgga
     2341 agcatcttga tgatggtgag ctgcttttca gcaggctcta tttccatcga ggctctgccg
     2401 gtgatgcgcg gcggtctgat ggagcctgag tcggccgacg gcgccgccgg gggggcttga
     2461 tcgttcttgc ctgcctgaag gtcctcggca tcgacctcca gtgtgtaaat cttgttcttt
     2521 acctctcgcg cgttttttgc gcccggtgca gacaggagat aatacttcag gttccggatt
     2581 gcctcagagt agagtccggc cttttcctgg gccataccga ggttgtagta cgcagcggcc
     2641 agccatggcg cggcattcgc cgccgcctcg aattcggaaa tggccttctt gtatccagcg
     2701 gagtccgcag ccctttgaaa acgcgctatg cccctggcca tgcttcgctc ggcgtcctcc
     2761 ggcacggcag gagcagg
//
LOCUS       DASSLD010000319         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_672050,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000319 DASSLD010000000
VERSION     DASSLD010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672050"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFK23_05745"
     CDS             <1..219
                     /locus_tag="VFK23_05745"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HET7318621.1"
                     /translation="ADRSYPDHHAFSRSDLAEVYKMSADHRADMIVTTEKDAVRLREM
                     GPEGIWALRIELVVNEQKEWERFLLNTL"
     gene            216..791
                     /locus_tag="VFK23_05750"
     CDS             216..791
                     /locus_tag="VFK23_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7318622.1"
                     /translation="MNSAVFLDRDGTVIEEVGYLSDLKQLRLIRGAAAAIRGLNDAGF
                     KVVLVTNQSGVARGYFTEAFVRETHDLLEKMLGLEGAHLDGVYYCPHHPKAGNSPYTA
                     ACDCRKPGTGMLEQAARDLGIDIRASFVVGDKWSDVELGQRAGAHSVLVGTGYGPDDP
                     GNERPAHLDDPDFTAGTITEAVEWILKTQRA"
     gene            850..1896
                     /gene="waaF"
                     /locus_tag="VFK23_05755"
     CDS             850..1896
                     /gene="waaF"
                     /locus_tag="VFK23_05755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HET7318623.1"
                     /translation="MKLDAKKILVMRYRFIGDTVLTVPFLRNLREAYPGARIDLMLEP
                     FSGQVIEGCPYVDRIIPFEIKTIHRYSSQSRRGKLAGYLRYWRQVRQERYDAAFVLKR
                     SLSSALLVWAAGVPRRIGFATEGRKILLSDPVAYRHDQHEVRNFLDCLRTLDIPVSSE
                     ALELWPAPGGDEKVRTLFHDAGFRLQDVKVLIHAAASLPSKQWPLERFAAVMGRIQKR
                     FHTRFVYTGAEADGALYQEIERQGAFGGLNLCGKTSLRENLSVYRATNLFFGVDSGPM
                     HMAAAVGVPVVALFGPTDERTWGPWGEGHTVITKRLSCRPCKPHKCRDNKCMREIGVE
                     EAAEAVEKKLASMR"
     gene            1907..>2413
                     /locus_tag="VFK23_05760"
     CDS             1907..>2413
                     /locus_tag="VFK23_05760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7318624.1"
                     /translation="MGKPKISVTVRTFNEEKNLRECLESVSWADEIVVVDSQSSDGTV
                     GIAREFTGRVLIQPWLGHIGQSQFATDRTSNLWVLHMDADERVTPGLRDEILALDLDN
                     SPFDAYEMPRRHFFMKRWIGHSAWYPDYKIRLFRKDRCKWGGYAPHDEVKVKGQKAKL
                     KNDILHYIY"
BASE COUNT          521 a          691 c          743 g          458 t
ORIGIN      
        1 gcggacaggt cgtatccgga ccaccatgct ttttcacgct ccgacctggc ggaggtctac
       61 aaaatgtccg ctgatcaccg ggcggacatg attgtcacca cagaaaagga cgctgtccgt
      121 ctgcgggaga tggggcccga ggggatatgg gcgctgcgga tcgaactcgt cgtcaatgaa
      181 cagaaggaat gggaacggtt tctcttaaac actctatgaa cagtgcggta tttctcgacc
      241 gggacggtac cgtcatcgag gaggtgggat acctcagcga cctgaagcag ctcagactca
      301 tccgcggggc cgccgccgca atcagaggcc tcaatgatgc ggggttcaag gtcgtgctgg
      361 tgacgaacca gtcgggcgtt gcgcgggggt attttaccga ggcctttgtg cgcgaaacgc
      421 acgacctcct cgaaaaaatg ctcggtctcg aaggagcaca tcttgacggc gtctattact
      481 gccctcacca tccgaaggct ggaaactcgc cctatacggc cgcatgcgac tgcagaaaac
      541 ccggcaccgg catgctcgaa caggcggccc gggatttggg catcgatatc cgggcatcat
      601 tcgtggtcgg agacaaatgg agcgacgtgg aactcgggca gcgcgcggga gcgcactccg
      661 tcctggtcgg gaccgggtat gggcccgacg atcccggaaa tgagcggcct gcgcatctgg
      721 acgatccgga cttcaccgcc gggaccatca ccgaagctgt tgagtggata ttgaaaacgc
      781 aaagggcata gcgcagagcg caaagcgtta agccttttcg ctatgctcta tgcgctatgc
      841 gcttacgtca tgaaactaga cgcaaaaaaa atactggtga tgcggtaccg tttcatcggc
      901 gatacggtgc tcaccgtgcc ctttctgcgt aacctgcgcg aggcctatcc cggggcccgg
      961 atcgacctca tgctggaacc gttttccgga caggtgatcg aaggctgccc gtacgtggac
     1021 cgcatcatcc ctttcgagat aaaaacgatc caccggtaca gcagccaatc caggcgcggc
     1081 aaactcgcag gatatctgcg gtactggagg caggtacggc aggagcgata tgacgctgca
     1141 ttcgtgctga aaagatcgct ttcgagcgcg ctgctggtct gggccgcggg agttcctcgg
     1201 agaataggct ttgccaccga gggcaggaaa atcctgcttt ccgatcccgt ggcctaccga
     1261 cacgaccagc atgaggtccg gaacttcctc gactgcctgc ggacactcga catccccgtc
     1321 tcttcggagg cgcttgaact ctggcccgct cccggcggcg acgagaaggt tcgcacgctc
     1381 tttcacgacg cgggattccg gcttcaggac gtgaaggtgc tcatccatgc tgccgcgagc
     1441 cttccctcga agcagtggcc cctcgagcgg ttcgccgctg tgatgggccg catacaaaaa
     1501 cggttccaca cgcgtttcgt ctataccggc gcagaggcag acggcgcgct ctaccaggaa
     1561 atagaacggc aaggggcctt cggcggtctg aacctctgcg ggaagacgag cttgcgtgaa
     1621 aacctctcgg tctatcgcgc gacgaacctg ttcttcgggg tcgactcagg ccccatgcac
     1681 atggcggcgg ctgttggcgt cccggttgtg gctctgttcg gtccaaccga tgaacgaacg
     1741 tggggcccct ggggggaagg ccacaccgtc atcaccaagc ggctgtcctg ccggccctgt
     1801 aaaccgcaca agtgccgcga caataaatgc atgagggaaa tcggcgtcga agaggcggcc
     1861 gaggcggtag aaaagaagct tgcgtcgatg cgataaggat tgtcacatgg gaaagccgaa
     1921 gatatcggtc accgtccgaa cgttcaatga agagaagaac ctccgtgaat gcctggagag
     1981 cgtttcctgg gccgacgaga tcgtggtggt agactcgcag agctcggacg gaacggtggg
     2041 catcgcccgt gagtttaccg gtcgcgtgct gattcagccg tggctcggac acatcggtca
     2101 gagccagttt gctacggacc ggacatcgaa cctctgggtg ctgcatatgg acgcggacga
     2161 acgcgtcacg cccgggcttc gcgatgagat cctggctctg gacctcgaca acagtccctt
     2221 tgacgcctat gaaatgccgc ggcggcactt tttcatgaag cgctggatcg ggcattccgc
     2281 ctggtatccg gactacaaaa tccgcctctt tcgcaaggac cggtgcaagt ggggaggcta
     2341 cgctccccac gatgaggtca aagtaaaggg tcagaaggcg aaactgaaga acgatattct
     2401 tcactacatc tat
//
LOCUS       DASSLD010000320         4819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1435197, whole genome shotgun sequence.
ACCESSION   DASSLD010000320 DASSLD010000000
VERSION     DASSLD010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435197"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..820
                     /gene="rplE"
                     /locus_tag="VFK23_05765"
     CDS             290..820
                     /gene="rplE"
                     /locus_tag="VFK23_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET7318625.1"
                     /translation="MKQRYHDVAVPALMKEFQYKNPMQAPKLVKVVLNMGMGEAIANA
                     KALDHAAEEMAKISGQRPVITRAKKSIATYKLREGMPIGCMVTLRRERMFEFLDRFIS
                     AALPRIRDFKGISSKSFDGRGNYSIGIKEQVIFPEIEIDKVESIHGMDIVIVTTAKTD
                     EEGRALLGHLGMPYRK"
     gene            831..1016
                     /locus_tag="VFK23_05770"
     CDS             831..1016
                     /locus_tag="VFK23_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET7318626.1"
                     /translation="MAKKSLIAKAQRPAKFKVRTYHRCPLCGRSRGYLRKFDMCRICF
                     RNRALRGEIPGVTKASW"
     gene            1037..1432
                     /gene="rpsH"
                     /locus_tag="VFK23_05775"
     CDS             1037..1432
                     /gene="rpsH"
                     /locus_tag="VFK23_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004820351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET7318627.1"
                     /translation="MMTDPIADMLTRIRNASKAKHEKVDIPSSRLKIEIAKILKDEGY
                     VKNVKLVKDRRQGLIRVYLKYTEDELPVIQGMKRVSRPGCRVYTGNDSIPKVMGGLGT
                     AILSTPKGIQTGKQAKKDNVGGEVLCHVW"
     gene            1478..2026
                     /gene="rplF"
                     /locus_tag="VFK23_05780"
     CDS             1478..2026
                     /gene="rplF"
                     /locus_tag="VFK23_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET7318628.1"
                     /translation="MSRVGRKPIEIPSGVEAKLETGKITVKGPLGEISQDVNPKLSIK
                     KQDNKLLVERPSDQKVYRELHGLTRNLIANMVTGVSKGYERTLEISGVGFKASAQGSN
                     LMLNLGFSHPIVYPLPKGIKATVDPKQTQIILKGVDKQLIGQIAANLRSLKKPEPYKG
                     KGIKYGSEVIHRKEGKAGKTGK"
     gene            2122..2484
                     /gene="rplR"
                     /locus_tag="VFK23_05785"
     CDS             2122..2484
                     /gene="rplR"
                     /locus_tag="VFK23_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET7318629.1"
                     /translation="MAQSKLKSRQRRHERIRKKVFGTADKPRLSIFKSLSHIYAQIID
                     DTSGKTLLAASTTEKEMKTGIKHGGNVEAAKKVGASIAARALGKNIKTVVFDRGGYQY
                     HGCVKALAEAAREQGLKF"
     gene            2559..3071
                     /gene="rpsE"
                     /locus_tag="VFK23_05790"
     CDS             2559..3071
                     /gene="rpsE"
                     /locus_tag="VFK23_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012939245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET7318630.1"
                     /translation="MAERYSNKEAEEGDGLKDKLVYLNRVAKVVKGGKRFNFSALVVV
                     GDGKGKIGVGKGKAAEAPVAIKKAVERAKKSMVLVSLKGDTIPHEILGHYGAGKVLLK
                     PASEGTGLIAGGASRSVLEVVGVRNVLCKSLGSSNPHNVVSATINGLTRLRSPEQVAS
                     LRGKTVEDLG"
     gene            3074..3268
                     /gene="rpmD"
                     /locus_tag="VFK23_05795"
     CDS             3074..3268
                     /gene="rpmD"
                     /locus_tag="VFK23_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET7318631.1"
                     /translation="MAKTGVKQLKITLVKSGIARPGKHKVVLIGLGLKKLNKSVIRED
                     TQQIRGMINKVSHLVKVEEA"
     gene            3305..3745
                     /gene="rplO"
                     /locus_tag="VFK23_05800"
     CDS             3305..3745
                     /gene="rplO"
                     /locus_tag="VFK23_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET7318632.1"
                     /translation="MRLEELKPVKGATKKSKRVGRGVGSGSGKTSGKGHKGQKARSGG
                     VKGAGFEGGQMPLQRRIPKRGFANIFRKEYSIVNLSDLDKIGGSDPVTPETLMQKGLI
                     KDLKSGVKVLGMGELKSKLTVRAHKFSKSALDKIQAAGGKAEVI"
     gene            3749..>4819
                     /gene="secY"
                     /locus_tag="VFK23_05805"
     CDS             3749..>4819
                     /gene="secY"
                     /locus_tag="VFK23_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET7318633.1"
                     /translation="MFTGLKNIFNIPELRKRIVFTLLMLAVYRVGAHIPTPGVKGEAL
                     SQFLHQSAAGLMGFFDMFSGGALSRVTIFALGIMPYITASIVLQLLTVVIPRLQALAK
                     EGEAGRKKITEYTRYGTVLISGFQSFWMAVGIEKMGGGAFVESTGWSFRILTMITLTA
                     GTTFIMWLGEQITERGIGNGISLIIFAGIVAGFPRAVTNTIGLVGSGEIAYFMVFIII
                     ALIIAVVAAIIVMERGQRKISIQYAQRMVGRKIYKGQSTHLPLKINSAGVIPPIFASS
                     ILLFPATIAGFVQVGWLQWFAQQLGPGKPLYTLIYVIMIVGFAYFYTAIVFNPVDIAD
                     NLKKYGGFIPGIRPGKKTSDYLF"
BASE COUNT         1260 a         1166 c         1482 g          911 t
ORIGIN      
        1 ggggcgacac ccgggtacgg gtgtgtaaga cctgcaacga ggccattgat taaaccgcgg
       61 ttccgtacag gccggttcgg aagagattat cagacaggaa agtagatcta tggcggataa
      121 aaaagacaaa ccaaaagaag aaaagagcgg agaccagaag aagacggccg ccaagccggc
      181 caagacggcc gcgaaaacca aggcccctgc agcgggcgca aaaaaggaag cggtcccggc
      241 ggagggagac aagaaaaaaa gcgcccccga gaaggttccg acgccccggc tgaagcagcg
      301 gtatcatgat gtcgccgtcc ctgcgctcat gaaggagttc cagtacaaga accccatgca
      361 ggcgccgaaa ctcgtcaagg tcgtgctgaa tatggggatg ggcgaggcta ttgccaatgc
      421 aaaggccctt gaccacgctg ccgaggaaat ggcaaagatt tcggggcagc ggccagtaat
      481 tacccgcgcc aagaaatcca tcgccacgta caagctgcgg gagggcatgc cgattggatg
      541 catggtgacg ttaaggcgcg agcgcatgtt cgagttcctc gaccggttca tcagtgcggc
      601 gcttccccgc atcagggact tcaagggcat ttcatcgaaa tcctttgacg gcaggggcaa
      661 ctactcgatc gggatcaaag agcaggttat tttcccggag atagagatcg ataaggttga
      721 gtcgatccac gggatggata ttgtgattgt tacgacggcg aagaccgatg aagaaggcag
      781 ggcgctgctc ggccacctag gcatgccgta tcggaagtag cggaggaacg atggcgaaga
      841 agtcactaat agcgaaggca cagagaccgg caaagttcaa ggtccgcacc taccaccgtt
      901 gcccgttgtg cgggcgatca cgagggtatc tcagaaagtt tgacatgtgc aggatctgtt
      961 tcaggaaccg cgctctccgg ggggaaatcc cgggcgtgac caaggcgagc tggtaaacaa
     1021 aaggggtttc aatacaatga tgaccgatcc gatcgcggac atgctgacgc gtatccgcaa
     1081 cgccagtaag gcaaagcatg aaaaagttga cattccttcc tcaaggctga agattgagat
     1141 tgcaaagatc ctgaaggacg agggatatgt gaagaatgtg aagctcgtga aggaccgcag
     1201 acagggcctg atccgggtat atctcaaata tacggaagac gagctgccgg tcattcaggg
     1261 catgaagcgt gtgagcaggc ctggttgtcg cgtctacacg ggcaatgatt caattcccaa
     1321 ggtcatgggt ggactgggga cggcaatcct gtcgacgccg aagggaatac agaccggcaa
     1381 gcaggcgaaa aaggacaatg tcggcggaga ggttctctgc cacgtgtggt aagtaacgag
     1441 cagagaatcc gttatcgaga agagtgtagg agctgttatg tcgagggttg gaagaaaacc
     1501 gattgagatt ccgtccgggg tcgaggcaaa gctcgaaacc ggcaagatta cggtgaaggg
     1561 tccgctgggc gaaatcagcc aggacgtaaa cccgaaactg tcgatcaaga agcaggacaa
     1621 taaattgctc gtcgagcggc cgtcggacca aaaggtttac cgcgagctgc acggcttgac
     1681 gaggaacctc atcgccaaca tggtgaccgg cgtgagcaaa ggctacgaga ggaccctgga
     1741 gatttcgggt gtcggattca aggcatcggc acagggatcg aacctgatgc tgaacctcgg
     1801 cttctcccac ccgatcgtct acccgctgcc aaagggcatc aaggcgacgg tggaccccaa
     1861 acagacgcag atcattctga agggcgttga caagcagttg atcggccaga tcgccgcgaa
     1921 tttgaggagc ttgaaaaaac ccgaaccgta caagggtaag ggcatcaaat acggttcgga
     1981 agtcattcac cgcaaggaag gcaaggccgg caagaccggg aagtaagaga agctaaaaca
     2041 gctcatagct cacggatcgc agcacctgcc tgcaatgcga actaccggct acgagcttta
     2101 tgaggagtca ggagaacgag cgtggcacag agcaagctga agtcgagaca gagaaggcac
     2161 gaaagaatca gaaaaaaagt gttcgggacg gccgacaagc ccaggctttc catatttaag
     2221 agcctgagcc atatctacgc gcagatcatc gacgacacat cgggcaaaac gcttctcgca
     2281 gcatcgacga cggaaaagga aatgaagacc ggcatcaagc acggcggtaa tgttgaggct
     2341 gccaagaagg tcggtgcgag tatagctgcg cgggcgttgg gcaagaatat aaaaactgtg
     2401 gtgttcgacc gcggcgggta tcagtatcat ggatgtgtca aggcgctcgc tgaagcggcg
     2461 cgggagcagg ggttaaagtt ttaagctctc ggctcattga tgatgatttt tccccatctg
     2521 cgagccatgg gctaaaagca agataatcgg aggcggcttt ggcagaacga tacagcaata
     2581 aagaggcgga agaaggcgat ggcctgaagg ataaattggt ctacctgaac cgggtcgcaa
     2641 aggttgtgaa gggcggcaag cggttcaatt tcagcgcgct cgtggtcgtc ggcgacggca
     2701 aaggaaaaat cggcgtcggc aagggcaaag ccgctgaggc gccagtggcc atcaaaaagg
     2761 ccgtggagcg ggccaagaag agcatggtcc ttgtgtccct gaagggggat acgattcccc
     2821 atgaaatact gggacactac ggcgcaggca aggtgttgct caagccggcc tccgaaggga
     2881 cgggcttgat cgcgggtggc gcgagcagat cagtgctcga ggtcgtgggg gtgcggaacg
     2941 tgctctgcaa atcccttggc tccagcaacc cccataacgt ggtgagcgcg acgatcaacg
     3001 ggttgaccag actgcggagc cccgaacagg tggcatcgtt gcgcggcaag accgtggagg
     3061 acttgggttg aacatggcga agacaggagt aaaacaactg aaaatcacgc tcgtgaagag
     3121 cggaatagcc cgtccgggaa agcacaaggt cgttctgatc ggactcggcc tgaagaaact
     3181 gaataaatcg gtgatccgcg aagacacgca gcagattcgc ggtatgatca ataaggtgag
     3241 ccacctggtc aaggtggaag aagcataaat caaccgcgac gttccaaacg cagggtcata
     3301 tactatgaga ctagaagagt tgaagcccgt caaaggggcc acgaaaaaat caaaaagggt
     3361 cggcagagga gtaggctccg gctccggaaa aacatcgggc aaggggcaca agggccagaa
     3421 ggcccgttcg ggcggcgtca agggcgctgg ttttgaagga ggccagatgc cccttcagcg
     3481 caggattccg aaaaggggct tcgccaatat tttccggaaa gaatattcca ttgtcaatct
     3541 gtccgacctc gataaaatag gcggttccga tcccgtaacg ccggaaacac tcatgcagaa
     3601 ggggctcatc aaggatttga agtcgggagt caaggtactc ggcatgggtg aactcaaatc
     3661 caagcttacc gtgcgtgcac ataagttcag taaaagtgct ctcgataaaa tacaggccgc
     3721 aggtggcaag gcagaggtga tataaccctt gttcaccggc ctgaagaata tattcaacat
     3781 tcccgagctc cggaagcgta ttgtgttcac gctcctcatg cttgcggttt accgagtcgg
     3841 cgcccacatc cctacaccgg gcgtgaaggg cgaggcgctg agtcagttcc ttcatcagtc
     3901 ggcggcaggg ctgatgggat ttttcgacat gttttccggc ggggcgctgt caagggttac
     3961 gatttttgcg ctcggcatca tgccttatat cacggcctcc attgtgctcc agctcttgac
     4021 ggtcgttatc ccgcggctgc aggcgctggc caaggagggc gaggcaggcc gtaagaagat
     4081 taccgagtat acccggtatg gtacggtttt aatatccgga tttcagtcgt tctggatggc
     4141 tgtcggcatc gaaaaaatgg gcggcggggc ctttgttgag tcaaccggat ggtcgttccg
     4201 cattctcacc atgatcacgc tgacggcggg caccacgttc atcatgtggc tcggcgagca
     4261 gatcacggaa cgagggatcg gcaacggcat ttccctgatc atttttgccg gcatcgtggc
     4321 cggatttccg cgtgcggtca ccaatacgat agggctggta ggctccggtg aaatagccta
     4381 tttcatggtc ttcatcatta tcgcactgat tatcgccgtc gttgctgcca tcatcgttat
     4441 ggagcgaggg cagcgaaaaa tctctatcca gtacgctcag cggatggtag ggagaaaaat
     4501 ttataagggg cagagcacgc acttgccgct caagatcaat tccgcgggcg tgattccccc
     4561 gatttttgcg tcctccatcc tgctttttcc cgcaacgata gcgggttttg tccaagtcgg
     4621 atggctgcag tggtttgccc agcagctcgg tcccgggaag ccgctctata cgctgatcta
     4681 tgtgatcatg atagtcggat tcgcctattt ctatacggcg attgtgttca atccggtgga
     4741 cattgcggac aatttgaaga agtacggcgg ctttattccg ggaataaggc cgggaaagaa
     4801 gacctctgat tatctgttc
//
LOCUS       DASSLD010000321         4142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_34014,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000321 DASSLD010000000
VERSION     DASSLD010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34014"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VFK23_05810"
     CDS             complement(<1..458)
                     /locus_tag="VFK23_05810"
                     /inference="COORDINATES: protein motif:HMM:NF025574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HET7318634.1"
                     /translation="MVIYDLYKLLKDELGNGSKDLVTRQSGEKIRRRIERDIEKESTG
                     SVVALDFSKIGVIDYSCADEIIAKLVSRLLAGEYGDKYLLLSGLNENQKENIEVAVER
                     KDLAVVAEMKDGGRAVLGNLNSYLRDTLDFIVKKKKATAKDLSDARKIEA"
     gene            complement(588..770)
                     /locus_tag="VFK23_05815"
     CDS             complement(588..770)
                     /locus_tag="VFK23_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006367239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318635.1"
                     /translation="MAITVKIEGGKLIITADLETPTPSASGKTLVVASTRGNKATDVM
                     IDGKPVIIGLNAYIKK"
     gene            complement(1192..2034)
                     /locus_tag="VFK23_05820"
     CDS             complement(1192..2034)
                     /locus_tag="VFK23_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7318636.1"
                     /translation="MNQLTEETFEQTLRSFIQAEQIDLRSVSFIDPYGMLGLLEIGEL
                     CTLEDVRKSIILPQEGDVCAYLDRMDFFPFAKRYFSLENFAPDPSGKYHRSPDSDVLL
                     EITPIQKSDDIHFIVGRVRDRAQAILATHLHYDDRAINGFIVALSEVCQNIIEHSENK
                     GFVGIQKYRFQNLSKNVVKIAVMDVGIGFRKSLSNRFKLKGDLDAIDKALLHGASRYE
                     DAGRGHGLAAVRRFVNQWNGKLSIRSGSAKLSIIPPWARGREQERGLITFPGSQINIM
                     LPEV"
     gene            complement(2164..2628)
                     /locus_tag="VFK23_05825"
     CDS             complement(2164..2628)
                     /locus_tag="VFK23_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013659705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HET7318637.1"
                     /translation="MNKISRAVILFIAQGAYSGRSPFAPGTAGTLVGILLYLGVKNLS
                     LIPYGILCVILCGIGTYTAGKAECMLGSKDSPSIVIDEIAGYLIAMFMVPSGWGYVAV
                     GFFLFRLFDVVKPWPLFGLQKVRGGLGVMLDDIGAGVYTNVALQIAAYYKIF"
     gene            complement(2834..3400)
                     /locus_tag="VFK23_05830"
     CDS             complement(2834..3400)
                     /locus_tag="VFK23_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HET7318638.1"
                     /translation="MEDEFVSREKKILTILEQEIEDGEEMSAAVRHIAERLLLEEKGY
                     ASSDVRKNVVFDVTLGAETVKSSVDFVVSLDNRIGMLIKCAPGSLDSRERHIVAAARV
                     FDVLPVPMAVVMDPVTAVVLDTATGKVAGEGFDAIPTKEQLRVLLAGKEPKPIAPEKL
                     EREKRVLLAFDAIRCCIPQGGQGGVSLE"
     gene            complement(3676..3990)
                     /locus_tag="VFK23_05835"
                     /pseudo
     CDS             complement(3676..3990)
                     /locus_tag="VFK23_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008552275.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
BASE COUNT          850 a         1157 c         1078 g         1057 t
ORIGIN      
        1 ttggcctcga tctttctggc gtccgagaga tctttggccg tcgctttctt ctttttcacg
       61 atgaaatcga gggtgtctct gaggtagctg ttgagatttc cgaggacggc cctgccgccg
      121 tccttcatct ccgccacgac cgcgaggtcc ttgcgttcga cggcgacctc gatgttctcc
      181 ttctggttct cgttcagccc ggagagaaga agatatttgt ccccgtattc gccggcaagg
      241 agcctcgata cgagcttggc tatgatctcg tcggcgcagg aatagtcgat aaccccgatc
      301 ttcgaaaaat cgagggcgac gacagagccc gtggactcct tctcaatatc ccgctcgatc
      361 cgccgccgga tcttctcccc cgactgccgc gtcacgaggt ccttggaccc gttgccgagt
      421 tcgtctttta aaagtttgta gaggtcgtag ataaccatga tatttttaca gagtgaatat
      481 aaatatagtc cgtcattccc gcaaaagcgg gaatccagtt tcttttttac attatcagtg
      541 ccaaacactc tggattcccg attgaaccat tcgggaatga cgttgagcta cttctttata
      601 tacgcattga gcccgatgat caccggtttt ccatcgatca tgacgtccgt cgccttgttc
      661 cccctcgtgc tggctactac cagggtcttc cccgacgcgc tcggggttgg cgtttccagg
      721 tctgctgtaa tgatgagctt gcccccttcg attttcacgg tgatggccat gatgttgcct
      781 cctcattatt tcattgaatc gttatggatg atcctctctc ttatagatct tcgttaatcc
      841 gccacgaaga acccgcgtgt agccgtcgat tgcctttttg ttggcctcat tcttttccgg
      901 agaaagactt ctccacccgg gattccgccg aaaataccgg taggttttcg tcttatcatc
      961 gacaaggtag accgccctgc ctattttcag ttcccgtggc attttcttct tccacgccct
     1021 gctttttaag gtcacaagtt gtgaccttaa atccttgccc acaacttttt aattcaattg
     1081 attcaattga accatatttt agaggttttg tcaaggggaa tattcattga aaatacagaa
     1141 tggatttccg ctttagcggg aatgacgaat cgtgtttctg aggatgattg attacacttc
     1201 cggcagcata atgttgatct ggcttccagg gaaggtaata aggccgcgct cctgttccct
     1261 tcccctcgcc catggaggga tgatggagag ttttgccgat cccgagcgga tggagagctt
     1321 gccgttccac tggttgacga acctgcgcac cgcggccagg ccgtggccgc ggcccgcgtc
     1381 ctcatagcgg gaggcgccgt ggaggagcgc cttgtcaatg gcatcgaggt cgcccttgag
     1441 tttgaacctg ttcgagagag acttccggaa cccgatgccc acgtccatga cagcgatctt
     1501 gacgacgttc ttgctcagat tctgaaagcg gtacttctga atgcccacga accccttgtt
     1561 ctcgctgtgt tctatgatgt tctggcagac ctcggataaa gctacgatga aaccgttgat
     1621 ggcccggtcg tcgtaatgga ggtgtgtcgc caggatggcc tgggcccggt ccctcaccct
     1681 tcccacgata aaatggatgt cgtctgattt ctggatcggt gtgatttcga gaagcacgtc
     1741 ggaatccggg cttcggtgat atttcccgga ggggtccggc gcgaagttct cgagagaaaa
     1801 gtatcgtttc gcgaagggaa agaagtccat ccggtccagg tacgcgcaca cgtcgccctc
     1861 ctgtggaagg atgatggact tgcgtacgtc ttcgagcgtg cagagctccc cgatctcgag
     1921 gagtcccagc atgccgtagg ggtcgatgaa ggaaacgctg cgaaggtcga tctgttccgc
     1981 ctggatgaag gaccggaggg tctgctcaaa ggtttcttcg gtgagttgat tcatgtttca
     2041 acagtacagg tcatataacc aaattgtcat tcccgaatgg ttctatcggg aatccatttt
     2101 gaatttgacc ttgaaaaaag ccaagggatt cccgttttcg cgggaatgac ggcctgggag
     2161 tcactaaaat attttataat acgcggctat ctggagcgca acgttcgtat agaccccggc
     2221 tccgatgtca tcgagcatga caccgaggcc gccgcggact ttctggagac cgaagagcgg
     2281 ccagggcttg accacatcga aaagacggaa gagaaagaac cccactgcaa catatcccca
     2341 ccctgacggg accataaaca ttgcaatcag gtatccggct atttcatcga tcacgatgga
     2401 agggctgtcc ttgctgccga gcatacactc cgccttgccc gcggtatagg tcccgatgcc
     2461 gcagagaatg acgcacagga tgccgtaggg gatcagtgag agatttttta caccgagata
     2521 gagaaggata ccgacgagcg tgcccgccgt gccgggcgca aaaggcgatc ttccggaata
     2581 ggcgccctgc gcgataaaaa gaattaccgc tctcgatatt ttattcatga actcctattt
     2641 ctggaaagac gtgaaaaggg gtaactactc aggctattgg atacgagttt aatatattcg
     2701 tgagatgtct caatccaccc cagaaacacc gagacgcaag caagaagcca agaagagtca
     2761 aaaggttttt tctccgtgcc ctcagcgtct cagcggttac aatcgacttt tacgaagtca
     2821 ccgcataccg accctactcc agcgaaacgc cgccctgccc tccctgcggg atgcagcagc
     2881 ggatggcgtc gaaggccagg agcacccgtt tctcgcgctc cagtttctcc ggggcaatag
     2941 gcttcggttc ttttcccgcg agaagaactc tcaattgctc tttcgtcggg atcgcatcga
     3001 acccctctcc cgcgaccttg ccggtcgccg tgtcgagcac caccgcggtt acggggtcca
     3061 tgacaacggc catgggaacg ggaaggacgt cgaacacgcg cgcggcagcg acgatatggc
     3121 gctcccggga gtcgagggac cccggagcgc acttgatcag catcccgatc ctgttatcga
     3181 gggacacgac gaagtccact gaggacttta cggtttcagc gcccagcgtc acatcgaaga
     3241 cgacgttctt gcggacgtcc gaggacgcat atcccttctc ttccaggagc agtcgttccg
     3301 cgatatgccg gacagcggcc gacatctctt cgccgtcctc gatctcctgc tcgagaatgg
     3361 tcagaatctt tttttcccgt gatacaaact cgtcttccat agcgcctttc atccctgact
     3421 gcaggccgga tcgggcgggc gttatttttt ctttcccgcc tttttcgcct tgacccgccg
     3481 tgccgttttt gccttcgccg gcttagtcga ggccttcttc ttgaggcctt tcttcttcgc
     3541 ggattttttc gccgccttct tcgtcttctt cgcgaccggt ttcttcgccg ctttcttccg
     3601 tcccgccgca gcggatttca tgagtgccgt ctctccggcg gaggctgtcg ccgccgggat
     3661 ctgaaggggc gacgggagga tgccgaggat gtcgcgctcg cggacaagga tgcgctcctc
     3721 tccgtcgatg ttatatgtct gtcccgcata cctgccgtat accacgcggt ccccgggctt
     3781 cacgaccgtc gggacgaaac ggcgctcttt tttcccgcct tttttcctcg tccgcttctc
     3841 ttcttcaaag gcaccgggac cgatcgcctc gataacgccc tcctgtggct tgtccttggc
     3901 tgtatcggga atgaagagcc cgcccgcggt cttttctgat ggttcagatg gaagaattac
     3961 agcccagtca tggagtggtc tcaccttcat ggttttcctc ccctttattg tcatccccga
     4021 tcgcctttat cgggaaaagg tcgtgcttca ttatacggga aaaaaatatc gctgtcaaaa
     4081 acaggatttg ccagtatcgg gtcaaacagg atgttgaaaa agtcgaaata cattggaatt
     4141 gt
//
LOCUS       DASSLD010000322         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1379021, whole genome shotgun sequence.
ACCESSION   DASSLD010000322 DASSLD010000000
VERSION     DASSLD010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379021"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..790
                     /locus_tag="VFK23_05840"
     CDS             101..790
                     /locus_tag="VFK23_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318639.1"
                     /translation="MSDAQLKALIVGKAFWVRNNVTGEQFSENFTAEGHTTVFHVGKY
                     ASVPSGYGNVLRDGYEGATIPYKIEGGKLITFISQDPYSVTVYKLGNVYYGARSNEFG
                     YANYEIVPSPQIAVNPITEVTNQFSIELGLTEQQKQQIVPILKEEITQLEALKKDTSL
                     SALKKVERLREIGSSFDEKLKPLINAEQQQKFQALREAFRRRLIEQMAEKAAQKLGGE
                     VKQWFTEKSSK"
     gene            987..2471
                     /locus_tag="VFK23_05845"
     CDS             987..2471
                     /locus_tag="VFK23_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HET7318640.1"
                     /translation="MNRLKKRLGNGALFAIVALALIAGCGKKEEAKVEKAAEKVVAEV
                     KADAKELEARETAIDAYIYAYPLVTMEITRRVITNVEKPVGSKAPMGQFARLRTYPAV
                     DDHSVTAPNADTLYTQIWLDVSKEPWILSIPDMKDRYFLFPMLDAWTNVFQVPGKRTT
                     GTKAQKYAITGPGWSGTLPAGLTEYKAPTDMVWVLGRIYCTGTPEDYKAVHALQDQVS
                     AVPLSAYGKPYTAVPGKVDPAIDIKAAPRDQVNAMDGAAYFKLFAELLKTNPPAAEDA
                     PMVAKLAKIGIVPGQDFDASKLDPAVAKGIADAPKPGQEKISAYLKESVVTGDSKVEH
                     GWLYFAHVGIYGTGYRNRAMITWYGLGANRLQDAVYPLSEGPELIKKYSGANKYVVHV
                     NKGEMPPANAFWSITMYDKDYFFVPNPINRYTVSSRNKFKTNADGSVDLYIQHDSPGK
                     GKEENWLPAPANEFVLLMRLYWPSEKPPSILDGTWKPPEVKEAS"
BASE COUNT          634 a          747 c          817 g          410 t
ORIGIN      
        1 ttggagctcg ccgatctggt acacgccgtc ggccgaggcc cgcaagaacg cggaggcagg
       61 gatgaccgtt gccgacctca agaagaaggg agcaacgccg ttgagtgacg cacaactgaa
      121 ggcgctcatt gtcggcaagg ctttctgggt gcgcaacaac gtgaccggag agcagttcag
      181 cgaaaacttc acggctgaag gtcacacgac tgtgttccac gtcggcaagt acgccagcgt
      241 gcccagcggt tatggcaacg tgttgcgcga cggctacgag ggagccacca tcccctacaa
      301 gatcgagggc ggcaagctca tcaccttcat ctcgcaggac ccgtactcgg tcacggtcta
      361 taagctcggc aacgtgtact acggcgcgcg cagcaacgag ttcggctatg ccaactacga
      421 gatcgttccg tcaccgcaga tcgcggtcaa cccgatcacg gaggtaacca accagttctc
      481 aattgagctg gggctaaccg agcagcagaa gcagcagatt gtccccatcc tgaaggagga
      541 gatcacgcaa ctggaggcgt tgaagaaaga cacgtcgctg agtgcattga agaaagtcga
      601 gcggctccgg gagatcggca gctccttcga cgagaagctc aagccgctga tcaatgccga
      661 gcaacagcag aagttccaag ccctgcgcga agccttccga cggaggctca tcgagcagat
      721 ggcagagaag gcggcgcaga agctgggagg cgaggtgaag cagtggttca cggagaagag
      781 cagcaagtaa caagataggg aagagatttt taacaggagg aaacggaggg aacggagaaa
      841 gatttaaaag aactttgtta tctccgttgc ctcatgttga agtctttggt tctcggataa
      901 gagcagcaag taaaaaggag gcgcgcaatg ccgcgtgttt cggcaggtcg cgatggaata
      961 taactaaaac tccggaggaa acggaaatga acagattgaa gaaaagactc ggtaacggcg
     1021 cgctcttcgc catagtggcc ttggccttga tagcggggtg cggcaagaag gaagaggcca
     1081 aggtcgaaaa ggcggcagag aaagttgtgg ctgaggtcaa ggcggatgcc aaggaactgg
     1141 aagcgaggga aaccgcgatc gacgcataca tctatgccta tccgctcgtg accatggaga
     1201 tcacgcgccg cgtgataacc aacgtcgaga agcccgtcgg atccaaggcg ccgatggggc
     1261 agttcgcacg cttgcgtact taccccgcgg tcgacgacca ctcggtcacg gcgccaaatg
     1321 ccgacaccct ctacacccaa atctggctcg acgtgtcgaa ggagccttgg atcctgagca
     1381 tcccggacat gaaggatcgc tatttcctgt tcccgatgct ggacgcctgg accaacgtgt
     1441 tccaggttcc tggcaagcgc acgaccggga ccaaggcgca gaagtacgcc atcaccgggc
     1501 ccggctggtc cggcacgctg cccgccggcc tcaccgaata caaggcgccc accgacatgg
     1561 tatgggtgct cggccgcatc tattgcaccg gcacgcccga ggactacaag gccgtgcacg
     1621 cgctgcagga ccaggtctcg gcggtgccgc tgtccgccta cggcaagccg tacacggcgg
     1681 tgcccggcaa ggtggatccg gccatcgaca taaaggcggc gccgcgcgat caggtcaacg
     1741 cgatggacgg cgcggcgtat ttcaagctct tcgccgaact cctgaagacc aacccaccgg
     1801 ccgccgaaga cgcgccgatg gtcgcgaagc tcgctaagat cggcatcgtg ccgggacagg
     1861 acttcgatgc gtccaagctc gatccggccg tggccaaggg catcgccgac gcgccgaaac
     1921 ccggacagga gaagatcagc gcttatctca aggaatcggt cgtcaccggc gactcgaagg
     1981 tcgagcacgg ctggctctac tttgcccatg tgggcatcta cgggacgggc taccgcaacc
     2041 gcgcgatgat cacgtggtac ggcctgggag cgaaccggct gcaggatgcc gtctacccgc
     2101 tgtccgaggg accggaactc atcaagaagt acagcggcgc gaacaagtac gtggtccacg
     2161 tcaacaaggg cgagatgccg cccgccaacg cgttctggtc gatcacgatg tacgacaagg
     2221 actacttctt cgtcccgaac ccgatcaacc gctataccgt gagctcgcgc aacaaattca
     2281 agaccaacgc cgacggctcg gtcgaccttt acatccagca cgattcgccc gggaagggca
     2341 aggaagagaa ctggctaccg gcgccggcga acgagttcgt gctgttgatg cggctgtact
     2401 ggccctccga aaagccgcca tccatcctcg acggcacctg gaagccgccc gaggtcaagg
     2461 aggcgagctg aggcaaaaga aggtcgaggg acgagtgacg aggggcaagt ctcactgaaa
     2521 cctgaaaccg gcaacttgga cttagacaga tgcgtgatgc gggctagcgc gatgcaggat
     2581 gcgagtcacg agatgcgaga tgaagggc
//
LOCUS       DASSLD010000323         4955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1207389, whole genome shotgun sequence.
ACCESSION   DASSLD010000323 DASSLD010000000
VERSION     DASSLD010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207389"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..900
                     /locus_tag="VFK23_05850"
     CDS             331..900
                     /locus_tag="VFK23_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318641.1"
                     /translation="MTGEKNRATCTFCGQPISGEPSEQPNPCEYCSSPSGGYPHLVIL
                     TEAIAGSAVGYVEGATYPQILLSIAKFLLGKKDDKLCGLATIMSHLACEVAIERSLSD
                     IFQRKGIQSLEETVADVLNGYNLGNDKVWNLYSTLTGDPIQEQPFWGSFLRSAKRRDN
                     IIRKGLIVGRADAEESIKAASDFLAHLPE"
     gene            complement(963..2378)
                     /locus_tag="VFK23_05855"
     CDS             complement(963..2378)
                     /locus_tag="VFK23_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HET7318642.1"
                     /translation="MAQATPQNTGREIPGLPGLLWDILRVGRGRPEGTVLRQHARLHR
                     LIEYARSRSSYYRERYAAFPRGICDLRELPPLSKSDLMANFDGWATDPAVTRETAEAF
                     IADPIRIGHLYLDRYVAFSTSGTTGTPAVILQDLGAMSVYQGLLLARRLPTLIAAGAF
                     RPFWRNRARTATIITTGGHFASSVVEALVRTRYPRLAGRNRTFSLMAPLPELVRGLNE
                     FRPAVVGSYPTALAVLAGEQAAGRLRIAPALLLSGAERLSTPLGKRISETFQCPLRDT
                     YAASEFMGIAFDCRFGRLHVNADWLILEPVDADGEPVAPGKASHTTLLTNLANLVQPL
                     IRYDLGDSVTVFPAACPCGSPLPAIRPEGRRDEILWIELADGAPRPLIPLVLATAVEE
                     APGLLRYQVLQAGPRRLRLRLEEAPGHDRAQVCGDVVHRLRAYLSSQGLASVEVELSE
                     ERPRSDTAGGKLRQFFVEPGG"
     gene            complement(2392..2913)
                     /locus_tag="VFK23_05860"
     CDS             complement(2392..2913)
                     /locus_tag="VFK23_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318643.1"
                     /translation="MKPGKGGTAMIRVKSFTAQLKIFHARLELDELDKEVCDFISSQG
                     IRKVISISDASTTGDKGETIGLIRVLTYEEPLAGSREHYQEKIESTLNEWGEEIDKIR
                     KKADLLGADARGRYREQIEDLRARQETARKKLEDLKRTGGEAWEDLRNGAEAALDELK
                     KGVEGAIAKLKKD"
     gene            complement(3218..3415)
                     /locus_tag="VFK23_05865"
     CDS             complement(3218..3415)
                     /locus_tag="VFK23_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318644.1"
                     /translation="MLAVVADDNFGPVPRQGNNRFRRLQRSEAMGNRQMMNIPVSPLV
                     ILKEAKQDSTPLMAEQSKGSE"
     gene            3472..4857
                     /locus_tag="VFK23_05870"
     CDS             3472..4857
                     /locus_tag="VFK23_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HET7318645.1"
                     /translation="MKKKYVNGASFREFARFSVISFRDLAVSAGPLILITAIVIVAAY
                     LYVRPAPPDTITIISGPPGSVFQRTAEKYRKILARNGVKLRILPSEGALENLKKLNDP
                     AVRVDVGFVQGGISTGIAVDKLVSLGSVFHEPLAVFYRNAVPLDRIYGLYGKRLAIGR
                     EGSGAHVLALILLKANGIEPGGATTLLALSGEGAAQALLERKVDAAFLMGDSAAPQIM
                     RKLLRTAGIRLLDIAQADAYVRRLPYLNKLDVPMGVFDFDQNLPPRDIHLIGPMVELV
                     ARDNLHPALSDLLIEAAREVHGGATLLQRAGEFPAPLEHEFRISENAKRFYSSGKGFF
                     YRFLPFWLASLADRTLVFVVPIAVLLIPGLKMVPKFYNWRVRSRIYRVYGKLIALERN
                     MLSQPAPKELEEMLKRLDDIETEVNKMKMPLAFADQFYVLRDHIDFVRGRYSTGGGPV
                     HGPMADRETNP"
BASE COUNT          954 a         1476 c         1501 g         1024 t
ORIGIN      
        1 gcctcctacg gcctgtggct cggcgtgatg gtcgtccgga ggattcgcgg gaaggaaagc
       61 accctcctca gagtccaatt ggagcatgcc ttccggcggt atttcgccgt cgtgttcccc
      121 ctcctcgttg tcgccgcggc ggtagagacg gccctcatcc tccggttgca gtaggaaagg
      181 ccgccaggaa ccggtgggcg gttccgtcct gcaaacgata tccctttccc tgcatctcat
      241 tgtaagggaa gcggacttgc aggatattcc cgctgaggaa gagacgatgg gcaattccat
      301 attccaagag acgccccggg gaatgactcg atgaccggcg aaaaaaaccg tgcgacctgc
      361 acattttgcg gtcaaccgat cagcggggaa ccctccgaac aacccaatcc gtgtgaatat
      421 tgcagttccc cgtccggagg gtacccgcac ctggtcatcc tgacggaggc aatcgccggg
      481 tccgcggtgg ggtatgtcga gggcgccacg tatcctcaga tactattgag tatcgcgaag
      541 tttctcctcg gcaagaaaga cgacaagttg tgcggcctcg cgacgatcat gtcgcatctg
      601 gcttgcgagg tcgcgatcga acgatccctg tccgacatct tccaacgaaa ggggatccag
      661 tctctcgaag aaaccgtggc cgacgtcctg aacggataca acctcggaaa cgataaggtt
      721 tggaatttat attcaacact gacgggagat ccaatccagg agcagccgtt ctggggaagc
      781 ttcctccggt ccgcgaaacg tagggacaat atcatccgca aggggttgat cgtcggcagg
      841 gcagacgcgg aagaatccat caaggctgca agcgattttc tcgctcactt gcccgaataa
      901 acttggtcgg accggtattg gaaagcgccg gctctgcaaa aacgttttcg gaggaggaac
      961 cgttagccgc cgggttcgac gaagaactgt cgcagttttc cccccgctgt gtcgctgcgc
     1021 ggcctttcct ccgataattc cacctccacc gacgcgaggc cctgggaaga caagtatgcc
     1081 cggaggcgat ggacgacgtc gccgcagacc tgcgcgcgat cgtgtccagg ggcctcctcg
     1141 aggcggagcc gaaggcgccg cggacccgcc tggagtacct ggtacctcag gagccccggc
     1201 gcctcctcca cggccgtggc cagcaccagc gggatcagcg gccgtggcgc gccgtccgcg
     1261 agctcgatcc acaggatctc gtcgcgccgg ccttcgggcc ggatggcggg gagcggactg
     1321 ccgcaggggc atgccgcggg gaagacggtg acgctgtcac cgaggtcgta acggatgagc
     1381 ggctggacca ggttggccag gttcgtcagc agcgtcgtgt gggaagcctt gcccggagcc
     1441 accggttcgc cgtcggcatc caccggttcc aggatcagcc agtcggcgtt gacatgcagg
     1501 cgtccgaacc ggcagtcgaa ggcgatgccc atgaattccg aggcggcata cgtgtcccga
     1561 agggggcatt ggaacgtttc ggaaatccgc ttcccaagag gtgtcgagag ccgttcggcc
     1621 ccggagagca ggagggccgg ggcgatccgc aggcgtccgg ccgcctgctc cccggccagg
     1681 acggccagcg cggtcgggta gcttcccacg accgccggac ggaattcgtt cagtccgcgg
     1741 acgagctcag ggaggggggc catcagggag aacgtgcgat tgcggccggc cagcctcggg
     1801 tagcgggtcc ggacgagggc ttcaacgacc gagctggcaa agtgcccccc ggtggtgatg
     1861 atcgtcgccg tccgagcccg gtttctccaa aagggtcgga aggcgccggc ggcgatcagc
     1921 gtcggcaggc gacgcgccag cagcaggccc tggtagacgg acatcgcccc gaggtcctgc
     1981 aggatgacgg cgggagttcc ggtcgtgccg gaagtggaga aggcgacgta ccggtcaagg
     2041 taaagatgcc cgatccggat tgggtcggcg atgaacgcct ccgccgtttc ccgcgtgacc
     2101 gccgggtcgg tcgcccaccc gtcgaaattc gccatcaggt ctgatttgga caatgggggg
     2161 agttcccgca gatcacagat gcctcgggga aatgcggcgt atcgctcccg gtagtaggac
     2221 gaccggctcc tggcgtattc gatcaaccgg tgaaggcggg cgtgctgccg taagaccgta
     2281 ccttccgggc gaccccgccc gacgcgcaag atatcccaca gcagccctgg caacccgggg
     2341 atttccctgc cggtattctg cggggtcgcc tgggccatcg ccgacgtccg gtcagtcttt
     2401 tttaagtttt gcgatggccc cttccacgcc ctttttcagc tcatcgaggg ccgcttccgc
     2461 accgttccga agatcctccc acgcctctcc gccggtcctc ttcaggtctt ccagtttttt
     2521 ccttgcagtt tcctgcctgg cccgaaggtc ttcgatctgc tcccggtatc tgcccctggc
     2581 atcagcgccc agcaggtcgg ccttctttct gatcttatcg atctcttcgc cccattcgtt
     2641 gagcgtggat tcgatcttct cctgataatg ttccctggat cccgccaagg gctcctcgta
     2701 agtcaggacc cggatcaggc cgatcgtttc acccttgtcc cctgttgtgg aagcatcgct
     2761 gatggagatc accttccgga tgccttggga agagatgaag tcgcagacct ccttgtccag
     2821 ttcgtccagt tccaggcggg catgaaagat cttgagttgg gcggtaaagg atttcaccct
     2881 gatcatggcg gttccccctt ttccgggttt catgaagcat tccagcgtgt ccgtcccaca
     2941 ccttaatgct accccaaatc cccgcatcgc atggggcaca cttcgggtat ggcagcgggg
     3001 gaagcgcgtt actcattcaa tggaacagtt cgggggaagc gactcagcgg cgttccctgt
     3061 aataacggtc tctatcgtaa tccctgtcat agtaatatcc cctttcgcgc tcatagcccc
     3121 ttccacccca atcgtcccga tagggatagt agaagcatcc ggacagcatc tgagccagaa
     3181 ataatacggt caggaaaatc agcaaggtat tcctcattta ttcgctccct ttgctctgtt
     3241 ctgccatcag aggcgtggaa tcctgttttg cctccttcaa tataacgagg ggggaaacag
     3301 gaatattcat catctggcgg tttcccatcg cttcagatcg ttgcaaacgg cgaaaacgat
     3361 tgttgccttg cctcggtacg gggccgaagt tatcatcagc gacaaccgcc agcacgcacc
     3421 tccggcaggc cggtccgctt ttaacccgat tgcggggaga gttcatcgga gatgaaaaag
     3481 aaatacgtca atggcgcatc gtttcgagaa tttgcccggt tctccgtgat ttccttccgc
     3541 gatctcgcgg tatcggctgg tcccctgatt ctgatcacgg cgatagtgat cgtggcggcc
     3601 tatctgtatg ttcgacccgc gccacccgac accatcacga tcatcagcgg gccgcccggc
     3661 agcgtcttcc agcggacagc cgagaagtac cggaagattc tggcgcggaa cggcgtgaag
     3721 ctccggatcc tgccatcgga aggggcgctg gagaatctca agaagttgaa cgatccggcg
     3781 gtacgcgtcg acgttggatt tgttcaaggc gggatctcta ccggtattgc cgtggacaag
     3841 ctggtttccc tcggtagcgt gttccatgag ccgcttgcgg ttttttaccg caatgccgtc
     3901 cccttggaca ggatttacgg gttgtacggg aaacggctcg cgatcggacg ggaaggcagc
     3961 ggggcgcatg tcctggccct gatactattg aaagccaatg gtatcgaacc tggtggcgca
     4021 acgacgcttt tggctctgag cggagaaggc gcggcgcagg cattgctgga gagaaaagtc
     4081 gacgcagcgt tcctcatggg agattccgct gccccccaga tcatgcggaa gcttcttcgg
     4141 acggccggta tccgcctgct cgatatcgcg caggcggacg cctatgtacg acgccttccc
     4201 tatctgaaca agcttgacgt acccatgggg gtgttcgatt tcgaccagaa cctccccccc
     4261 cgggatatcc atctgatcgg ccccatggtc gagctcgttg ctcgtgacaa cctgcatccc
     4321 gcactgtccg atctgttgat cgaagcggcg cgagaagtgc acggcggcgc aaccctcctg
     4381 cagagggcgg gcgagtttcc ggcccctctg gaacatgaat tccgcatcag tgagaatgcg
     4441 aaacgtttct acagttcggg gaagggcttc ttctatcgct tcctgccgtt ttggctggca
     4501 agcctggcgg accgtacgct ggtatttgta gtgccgatcg cagtcctgct gatcccgggg
     4561 ttgaagatgg tgccgaagtt ttataactgg cgagtcaggt cgcgcatcta tcgggtgtac
     4621 ggtaaattga tcgcgctcga gcgcaacatg ttatctcagc ctgcgcccaa ggaactggaa
     4681 gagatgctca agcggctcga cgacatcgag accgaagtca acaagatgaa aatgccgctg
     4741 gcgtttgccg atcagttcta tgtactgcgg gatcatatcg atttcgtgcg cggcagatat
     4801 tcgactggcg gtggccccgt tcatggtccg atggcggacc gggaaacgaa tccgtgatgg
     4861 gttttcatcg ttgccagccc cttgtcgaaa tgcgtttccg ccatcaatct ccccgacagg
     4921 aggtgcgtct ggaagtcgag aacgatctcg ttcaa
//
LOCUS       DASSLD010000324         5464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_135297,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000324 DASSLD010000000
VERSION     DASSLD010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135297"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VFK23_05875"
     CDS             <1..729
                     /locus_tag="VFK23_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7318646.1"
                     /translation="PPLPHSPSPAIIICHGVGANKSDFTEFAIALSRRGYEVLLFDFR
                     AHGTSGGSRTSLGYREQKDVFAALGYLKTRQEIDPHRIGIYGFSLGGSTAILAAEKSR
                     GFRAVVADSAFTSLKDMARTAITGVYHLPAFPFLPLTVLGYELYFQTSIDAVSPVMAI
                     AGISPAPVLIIAGEGDELVPVDNGRRLYAAAKEPKEIWIIPGAGHGGTIAAAESEYEK
                     RVGEFFDKNLTNVEAGQGGIGKTE"
     gene            815..1519
                     /gene="tsaB"
                     /locus_tag="VFK23_05880"
     CDS             815..1519
                     /gene="tsaB"
                     /locus_tag="VFK23_05880"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET7318647.1"
                     /translation="MYILGIETSTKTGSVAVVSDKAVIAQYSLNIEITHSERLMSTVD
                     RVLKDTGIALSQLDGFAVATGPGSFTGLRIGVSTVKGLAFATDKPAAAVPTLYALAWN
                     VPHAAHPVCPLLDARKNEVYAAVYTSDGPVLSQTVPEAVVPLSRLGDLVAGRAVFTGE
                     AAILYRGEIERMFGEKALFAPHSAILPAAAAVAEIGLGMIRAGRQADIDSLAPKYIRR
                     PEAEVAWERMQSRETS"
     gene            1516..1986
                     /gene="rimI"
                     /locus_tag="VFK23_05885"
     CDS             1516..1986
                     /gene="rimI"
                     /locus_tag="VFK23_05885"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET7318648.1"
                     /translation="MRKEDIDQVLAIEQASFSMPWSRNLFLSEFRNPRVSSLMVSLAD
                     GPVRKVSGFIVFWTVEDEMHILNLAVAPQFRRQGIARKLVLTALSHARSKGAKRAFLE
                     VRASNTAAQKLYSSLGFTGVAIRRAYYDTPVEDAVVMAIERGAFESLVKGLQNP"
     gene            2120..2359
                     /locus_tag="VFK23_05890"
     CDS             2120..2359
                     /locus_tag="VFK23_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HET7318649.1"
                     /translation="MNVMEVSEEALRAKLRMEDPEFQKWEEEHHKLESTLASIDSHVY
                     LTPEEEVERKRIQKLKLAAKDKMMDLIRRRKVGRA"
     gene            2528..4180
                     /gene="ilvD"
                     /locus_tag="VFK23_05895"
     CDS             2528..4180
                     /gene="ilvD"
                     /locus_tag="VFK23_05895"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET7318650.1"
                     /translation="MRSDRIKKGLERAAHRSLLFATGITRRELDKPMIGVATSWNDLI
                     PGHIGMRDLERFIEKGVHTGGGWPLFFGVPGICDGIAMGHMGMHYSLASRELIADIVE
                     SVAMAHAFDGMVLLTNCDKITPGMLMAAARIDIPTIVVTAGPMHSGNYRGNRVNLISA
                     FEAVGKVRKGEMSETDLAELEVCACPGPGSCQGMYTANTMACVTESLGMSLPGCATAL
                     AVSGEKRRIAFESGKRIVGMVRENLTPRKIMTKNAFENAIRIDMALGGSTNTTLHIPA
                     IAYEAGIELSMDTFDKISRTTPHVCNMLPGGHHYLEDLDAAGGIPGVLSRFKGKLKGS
                     PTVSGKSIVTLAKAGRVGNEDVIRTLDKAYHKEGGIAVLSGNLAPNGSVVKQTAVSDK
                     MMRFEGTAVVFDSEEESMKAILAGKIKAGHVVVIRYEGPKGGPGMREMLSPTSAISGM
                     GLGESVVLLTDGRFSGGTRGPCIGHISPEAAEGGPIAIVRNGDRIVLDIPNRKLDIKL
                     SKAEIKRRLAKWKPREPKIKSGWLARYAAVVTSANTGAIVKA"
     gene            4292..>5464
                     /gene="ilvB"
                     /locus_tag="VFK23_05900"
     CDS             4292..>5464
                     /gene="ilvB"
                     /locus_tag="VFK23_05900"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HET7318651.1"
                     /translation="MSIKEMTGAEILLECLKKEKVDTLFCYPGGVVLKIFDVLYEQKD
                     IRMILPRHEQGGVHMADGYARSTGKVGCALVTSGPGATNTVTGLATAYMDSIPIVVIT
                     GQVPTALIGNDAFQEADIVGITRPCTKYNFLVKDVKDLARTIREAFYLAASGRPGPVL
                     IDLPRDVVTNKAEFHWSEKIDIRSYRPSVTGNKWQIKQAAEAIAKAKKPVLYIGGGVI
                     LSNAAPEILEFAELTRIPVAHTLMGVGGFPGTHPLSLGMLGMHGTYFANMSVHNSDLI
                     IAIGARFDDRVTGKVEGFAPEAKIIHIDVDPTSIRKNVRVDIPVVGDVKHVMTDLNKE
                     VRAIKEPWEAIRKSWLKQIDAWRAERPLSYEYSDEVIKPQHVVEKLYELTGGDAIIV"
BASE COUNT         1216 a         1526 c         1641 g         1081 t
ORIGIN      
        1 ccgccccttc cacactcgcc gtctcctgcc atcatcatct gccacggcgt cggcgcgaac
       61 aaatcggatt ttaccgagtt cgcaatagcc ctctcgcgcc ggggatatga ggtcctcctg
      121 ttcgactttc gcgcccatgg aacaagcggc ggaagccgta cttctctcgg ttatcgcgag
      181 cagaaggacg ttttcgccgc gctcgggtat ctgaaaacgc ggcaggaaat cgatcctcat
      241 agaatcggaa tatacggctt ttccctaggc ggttcaaccg cgatcctcgc cgcggagaag
      301 tcccgggggt tccgcgctgt tgttgccgac agcgccttca caagcctcaa agacatggcg
      361 cggaccgcga tcaccggggt ctatcatctt ccggcctttc cctttctccc tctcacggtc
      421 ctcgggtatg aattgtattt ccagacatcc atcgatgcgg tctcgccggt aatggcgatc
      481 gcaggcatct cacccgcgcc ggtcctgatc atcgcgggcg agggagacga gcttgtccct
      541 gtagacaatg ggcggagact gtatgccgcg gcaaaggaac cgaaagagat atggatcatc
      601 cccggtgctg gtcacggcgg cacgatcgcc gccgcggaaa gcgagtatga aaagagggtg
      661 ggggagtttt ttgataaaaa cctgacaaac gttgaagcgg ggcagggggg gatcggaaaa
      721 acggagtaaa ggaattgtct ctccaccgat tcacgcatcc ccgttttcgg ttttcctttt
      781 gacatggtct cttttttccg gtaaggtgtc gaccatgtac atcctcggca tcgaaacgtc
      841 aaccaagacc ggcagcgtgg cggtcgtttc tgacaaggcc gtaatcgcgc agtacagcct
      901 caatatcgag atcacgcatt ccgagagact catgtccacc gtggaccggg ttctcaagga
      961 cacgggcatt gccctgtcgc agctcgacgg ttttgccgtt gccacggggc cggggtcctt
     1021 cacggggctc cgcatcggcg tctcaacggt caaggggctt gcctttgcca cggacaaacc
     1081 ggcggctgcc gtaccgacgc tctatgcgct tgcgtggaac gtcccccatg ccgctcatcc
     1141 cgtctgcccc ctccttgatg ctcgcaagaa cgaagtctat gcggcggtct atacatcgga
     1201 cggcccggtc ctcagccaga ccgtgcccga ggcggtcgtt cccctctccc ggctcggcga
     1261 tctggtcgca ggtagggctg tgttcaccgg agaagcggcc attctttacc gcggcgagat
     1321 tgaaagaatg ttcggcgaaa aggcattgtt tgcgccgcac tccgctatcc tgcctgcggc
     1381 agcagcggtg gcggagatcg gcctcgggat gatccgggcg ggcaggcagg cagatattga
     1441 cagccttgcg ccgaaataca ttcgcaggcc ggaggcagag gtggcgtggg aaaggatgca
     1501 atcacgagag acttcatgag gaaggaagac atagaccagg ttctcgccat cgaacaggca
     1561 tccttcagca tgccctggtc ccgcaacctc ttcctctcgg agttccggaa cccgcgtgtc
     1621 tccagcctca tggtctcgtt ggcagacggc ccggtccgca aggtctccgg atttatcgta
     1681 ttctggaccg ttgaggacga gatgcacatc ctgaacctcg ctgtggcgcc gcagttcagg
     1741 cggcagggta tcgcaagaaa actcgtgctt acggcgctct cccacgcacg cagcaaagga
     1801 gcgaaacggg cctttctcga ggtcagggca tcgaataccg ccgcccaaaa gctctattca
     1861 agcctcggat ttaccggcgt tgcaattcgg cgagcgtact atgatacgcc tgttgaagac
     1921 gcagtcgtta tggcgatcga gcgaggtgca tttgagagcc tggtaaaagg tcttcagaac
     1981 ccataactgg ctgttttaac gagtttaatt tcctattgcc ttttccggcg aagtatgata
     2041 attttcaatt gtaattaaaa ccgctctttg tacatgcctt ctcggatcat aataaacctg
     2101 acgataagga gggctgctta tgaatgtcat ggaggtaagc gaagaagcac tgcgggcaaa
     2161 actcaggatg gaggacccag agtttcagaa gtgggaagaa gagcaccata aactggagtc
     2221 aacactggcg agcatagatt cccatgtgta tctcactcct gaggaagaag tggaacggaa
     2281 aaggatccag aagctgaaat tggcagccaa ggacaagatg atggatttga tcaggcgcag
     2341 gaaggttgga cgggcctgat gcggcaaagg caaggtaaaa aaccttgcct tttgtaattt
     2401 gaagtccgaa actgagaatc cctctttacg aagagatgta gagagggatt tttttattga
     2461 ctctaatcgt ttatctatgt taaaaataag gataatcggc ttgtaaagcg ttgtaaggag
     2521 cacgtttgtg agaagcgaca gaatcaagaa aggcctggag cgcgcagcgc ataggtctct
     2581 tttattcgct accggcatca cgcggcgtga gctcgataag cccatgatcg gcgtggccac
     2641 aagctggaac gacctcatcc cgggacacat cggcatgcgc gacctggagc ggttcatcga
     2701 aaagggcgtt cataccggcg gcggctggcc cctctttttc ggagtaccgg gcatctgcga
     2761 cggcattgcc atgggccaca tgggcatgca ctattcgctg gcctcgcgcg aattgatcgc
     2821 ggatatagtg gagtccgtgg ccatggccca cgccttcgac ggcatggtgc ttctgacgaa
     2881 ttgcgacaag atcacaccgg gaatgctcat ggctgcggcg cggatcgata tcccgacgat
     2941 tgtcgtgacc gccggtccca tgcattcagg gaactaccgc ggaaaccgtg ttaacctgat
     3001 cagcgccttc gaggccgtgg gcaaggtccg gaagggcgag atgtcggaaa ccgacctcgc
     3061 cgagctcgag gtctgcgcct gcccggggcc cggctcctgc cagggcatgt atacggcaaa
     3121 cactatggca tgcgtcaccg agtcgctcgg catgagcctg cccggatgcg ctacggcgct
     3181 tgccgtttcg ggcgagaagc ggagaatcgc tttcgagagc ggcaaacgca tcgtcggcat
     3241 ggtaagggag aacctcacgc cgagaaagat catgacaaag aacgccttcg aaaacgccat
     3301 tcgcatcgac atggccctcg gcggctccac gaatacaacg ctccacatcc ccgcgatagc
     3361 gtacgaggca ggtatagaac tgtcgatgga tacgttcgac aagatcagcc gtacgacgcc
     3421 gcatgtttgc aatatgctgc cgggcggcca tcactatctc gaagacctcg atgcggcagg
     3481 cggcattccc ggcgtgctgt cccggttcaa gggcaagctc aagggttcgc cgaccgtgag
     3541 cggcaaatcc atcgttacac tggctaaggc aggccgcgtg gggaacgagg acgtcatcag
     3601 gacgctcgat aaggcctatc ataaagaggg cggcatcgcc gtgctttcgg gcaatctcgc
     3661 gccgaacggg tccgtggtca aacagaccgc ggtcagcgac aagatgatga ggttcgaggg
     3721 cacggccgtt gtattcgact ccgaggagga gtccatgaag gccattctcg cgggcaagat
     3781 aaaggcgggt catgtggtcg tcatccgcta tgaagggcca aaaggcggac cgggtatgcg
     3841 tgagatgctt tcgcccacgt cggccatatc cggcatgggg ctcggtgagt cggtagtcct
     3901 gctcacggac ggcaggttct cgggcggcac gcgtggcccg tgcattggcc atatctcacc
     3961 cgaggcggcg gagggcgggc cgatagcgat cgtgcggaac ggggacagga tcgttctcga
     4021 tatcccgaac cgcaagctgg acatcaagct gtccaaagcg gagatcaaga ggcggctcgc
     4081 gaagtggaaa ccgcgtgaac cgaagataaa atcgggttgg ctcgcacgat atgcagcagt
     4141 ggtcacgtcg gccaataccg gggcgatcgt aaaagcgtaa aatgctaaag gtcagaaggt
     4201 aggaaggtgt gaaggtatga aacagcggta gggtttttct taccttccta acttctttcc
     4261 atcctacctt ctgaactaca cagggtgaac tatgtcaatc aaagaaatga ccggcgctga
     4321 aatcctcctt gaatgtctga aaaaggagaa agtcgatacg ctgttctgct accccggcgg
     4381 cgtcgtgctc aagatcttcg acgtccttta tgagcagaag gacatccgga tgatcctgcc
     4441 tcggcatgag cagggcggcg tgcacatggc ggacggctac gcccgttcca cgggaaaggt
     4501 cggctgtgcc ctggtcacgt ccggccccgg ggcgacgaac acggtcacgg gccttgccac
     4561 ggcctacatg gattccatcc ccatcgtggt cattacgggg caggtcccca cggccttgat
     4621 cggcaacgac gcgttccagg aagcggatat cgtgggcatt acgaggccgt gcacgaagta
     4681 caatttcctc gtcaaggacg tgaaggacct tgcgcggacc atccgcgagg ccttttatct
     4741 cgccgcgagc ggccgtcccg gtcccgttct aatcgacctg ccgcgcgacg tcgtcacgaa
     4801 taaggcagag ttccactggt ccgagaagat cgatatccgc agttacaggc cgtcggtcac
     4861 cgggaacaag tggcagatca agcaggcggc cgaggcaatc gccaaggcca agaaacccgt
     4921 gctctacata gggggcggtg tgatcctttc gaatgccgcg ccggagatcc tcgagttcgc
     4981 cgaactgacg aggatccctg tggcccacac actcatgggc gtgggaggtt tccccgggac
     5041 ccacccgctg tcgctcggca tgctcggcat gcatggcacg tacttcgcaa acatgtcggt
     5101 ccacaactcg gacctcatta tcgccatcgg ggcgcgcttc gatgaccggg tgaccgggaa
     5161 ggtggaaggg tttgcccccg aggccaaaat catccatatc gacgtcgatc cgacgtccat
     5221 cagaaagaac gtgcgcgtgg atatccccgt cgtgggcgac gtcaaacacg tgatgacgga
     5281 cctgaacaag gaagtgaggg ccatcaagga gccgtgggag gcaatccgca agagctggct
     5341 caagcagatc gacgcatgga gggccgagcg tcccttgagc tacgaatatt cggacgaggt
     5401 gatcaagccg cagcacgtgg tcgaaaaact ctacgaactc accggcgggg acgccatcat
     5461 cgtg
//
LOCUS       DASSLD010000325         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_622271,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000325 DASSLD010000000
VERSION     DASSLD010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622271"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="VFK23_05905"
     CDS             complement(<1..1299)
                     /locus_tag="VFK23_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7318652.1"
                     /translation="MKSLLKEATEQKKYEISKHLYESIMDGITSGVWVADKNDVIYYA
                     NRAMEMIAGVTQQKLIGSMVFPDPAEQATKHFKPHYREAQASLRPVYYSEVPILTSAG
                     RQTYQSGWLIPIIENGNYDGMICTVEDITNEKQTRKALKESQAKYKQLVESANSIILR
                     MDGKGVITFINPFAQKYFGYSETEIVGKNIMGTIVPETENPGPDMQTMLQNIGANPEQ
                     HANTEFEHIRRNGETVWISWANTALFDDSGTMTEVLCVGNDVTLQKRHEDLLKKWSSD
                     LEERVTIGTARLSIANEELQQEIAERKWMEQSLRKSEEKYRRVVEYANEGILITQDNY
                     FQYVNPKAVKIWGHSREELTSRPFTDFIHPDDREMVMERHLKRIKGDSVSPYYFCRIL
                     DGEGNTKWLEINSVLITWMGRPATLAFFNNITERKMAEEKL"
     gene            complement(1353..1640)
                     /locus_tag="VFK23_05910"
     CDS             complement(1353..1640)
                     /locus_tag="VFK23_05910"
                     /inference="COORDINATES: protein motif:HMM:NF021758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HET7318653.1"
                     /translation="MKRLSILYCGTCNYRPIAAGLSRAIETETGIKPVLIHSQEMGAF
                     EISVNDELIFSKHVSGRFPDFAEIVEIVKKKTEPDERHAMPPGAPEKSSGQ"
     gene            1797..>2264
                     /locus_tag="VFK23_05915"
     CDS             1797..>2264
                     /locus_tag="VFK23_05915"
                     /inference="COORDINATES: protein motif:HMM:NF015427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HET7318654.1"
                     /translation="MSRAGSRPCGGIFDTPAKEARLEELTRLTETTGFWDSKDKANKV
                     LKEKAALERALRDWNALARRLSDLEAMAELASEEGGEAMRGELERELEAIRRDIASAE
                     VAALLSGEQDASNAIVSIHPGAGGTEAQDWAEMLMRMYLRWAEQRGYKTEILDY"
BASE COUNT          429 a          631 c          622 g          582 t
ORIGIN      
        1 cagcttctct tcggccatct tccgctccgt gatgttattg aaaaaggcga gcgttgccgg
       61 cctgcccatc caggtaatca ggacagagtt gatctccagc catttggtgt tcccttcccc
      121 gtcgaggatg cggcagaaat aatagggtga aacgctgtcc cccttgatcc tcttcaggtg
      181 ccgctccatc accatctccc ggtcgtcggg atgaatgaaa tcggtaaaag gccgcgaggt
      241 cagttcttcc cgggaatgtc cccagatctt taccgccttg gggttcacgt actggaagta
      301 attgtcctgc gtgatcagga tgccttcgtt cgcatactcg acgacgcgcc ggtatttttc
      361 ctccgatttc cgcaggctct gctccatcca tttgcgttcg gcaatttcct gctgcagttc
      421 ctcgttcgcg atgctgagcc gcgcggtgcc gatcgtcacc cgttcttcca ggtcgctgct
      481 ccactttttc aggagatctt cgtggcgttt ctggagcgtc acgtcgttgc cgacgcagag
      541 tacctcggtc atggttccgg agtcatcgaa aagcgccgtg ttcgcccacg aaatccagac
      601 ggtctcgccg tttctccgga tatgctcgaa ttcggtgttc gcgtgctgct cgggattggc
      661 cccgatgttc tgcagcatgg tctgcatgtc gggaccgggg ttttctgttt ccgggacgat
      721 ggttcccatg atattttttc cgacgatctc cgtctcgctg tatccgaaat atttttgcgc
      781 aaagggatta atgaaggtaa tgacgccttt tccgtccatc cgcagaatga tgctgttggc
      841 gctctcgacg agctgtttgt atttagcctg actctccttc agcgcctttc gcgtctgttt
      901 ctcgttcgtg atgtcctcta ccgtgcagat catcccgtca tagtttccgt tctcgatgat
      961 ggggatgagc catccggact gatacgtctg cctccctgcc gaggtaagga tggggacttc
     1021 ggagtaatat acgggacgca acgatgcctg cgcctcccgg tagtggggct taaaatgctt
     1081 cgttgcctgc tcggcggggt cgggaaatac cattgacccg atgagctttt gctgcgttac
     1141 cccggcaatc atctccatgg ccctgttggc gtagtagatg acgtcgtttt tatcggccac
     1201 ccagacaccg gaggtaatgc cgtccatgat gctttcatag agatgcttgc tgatttcgta
     1261 ttttttttgt tccgtggctt ctttgagcag agatttcatg gttcctcacc gctgaacccg
     1321 tcctcgcatg aacgggccga acttctcgtg acctactgcc ccgacgattt ttcaggcgcc
     1381 cccggcggca tggcatgcct ctcatcgggt tccgtttttt tctttacgat ttcgacaatc
     1441 tccgcaaagt cgggaaagcg tcccgaaaca tgtttggaaa atatcagttc gtcgttcacc
     1501 gaaatttcga aggcgcccat ttcctgagaa tggatgagga cgggttttat cccggtttcg
     1561 gtctctatgg ctctggaaag gcctgcggct atcggccggt aattgcaggt cccgcagtag
     1621 agaatggata atcgcttcat cgataataac gttaacactg tgcatgtttc ctgtcaatac
     1681 agctttcacg ttttggtttt gattgtttcc tgttactatg gtataatccg cgcagttttc
     1741 atcatctata gaggagagcg gcatgctttt agaagacctc aagcagcaac ttgtcgatgt
     1801 caagggcagg atcgagaccc tgcggaggca tctttgacac tcccgccaag gaagcccgtc
     1861 tggaagaact tacccgcctc acggaaacca ccggtttctg ggacagcaag gacaaagcca
     1921 acaaggtctt aaaggaaaag gcggcccttg agcgcgcgct cagggactgg aacgcgcttg
     1981 cccggcgcct gagcgacctc gaggccatgg cggagttggc ctccgaggaa ggcggcgaag
     2041 ccatgcgcgg tgaactggag cgcgaactcg aagcaatccg gagggacatc gcgtccgccg
     2101 aggttgcggc gctcctctcc ggagagcagg acgcctcgaa cgccatcgta tcgattcatc
     2161 ccggggcggg cggcaccgag gcccaggact gggccgagat gctgatgcgc atgtacctgc
     2221 gctgggccga gcagcgcggg tacaagaccg aaattctcga ctac
//
LOCUS       DASSLD010000326         3220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_962200,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000326 DASSLD010000000
VERSION     DASSLD010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962200"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /locus_tag="VFK23_05920"
     CDS             complement(<1..1588)
                     /locus_tag="VFK23_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7318655.1"
                     /translation="MLYLFGFLVVTALAIVLQLQYRGFEGPLSDNLLILFLINANFLL
                     LAAVVFMIARSLWKLSIERKHGVLGARFRTKLVFAFVGLSFIPPILLFIIGSGMFTTS
                     IERLFSLRIENSLKDSVSLAQEYYGRLRKDALVFGKQISLQMTEGRLLSRFDNVVITE
                     YLAKKAKEYGLGSVELFTAPQRRKAVIVTEQFPPQAFSQTPPELVALAFAALDAADLS
                     GTAKKGDIMRVVVPVHETPEGNKVVAAVAVSYYIPPNLAAKAEEIHEGYAQYRSAFKS
                     KEPIKLAYRLGFLAVTLALLLSAIWVALRVAAGITVPIRKLAEGTAAVAAGKFDYQID
                     EKSGDEVGVLVDSFNVMTRELKSSREQLEQEINYKKTILSNIDTGVVSIDRAGRITTV
                     NQSASEILGIREADVLGKRYDAAFGFIELDPIRGLFRKLEPGEGSAEKELSLTVRGRV
                     LTIRLRITTLRDGGGSPIGSVVTFDDMTELLRAKKAETWQDVARRIAHEFKNPLTPIK
                     LSAERLRKKKTEGAPDFDAVF"
     gene            1910..3151
                     /locus_tag="VFK23_05925"
     CDS             1910..3151
                     /locus_tag="VFK23_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7318656.1"
                     /translation="MTIKTNVLIQAACVSLLLALLPGTAAPQEPKETGQQPAQASGGP
                     EQLKSEAVTTPSAGAAEPQPKQEAAPAIKAEEAAPAAEKELLYTIKRGDTLWDISNTF
                     FKDPFLWPFIWKANPPITNPDLIYAGNKLVIPSLAPIERAIKAPAAAAPTEEQAPGPE
                     TAAAPHALPKPPAGVTEEAPAGGGKIIVPEEEAQPVVDKYSFLSAGFVNDAESVGTII
                     GPAVQEKTIMGYDDVVYVRFGADNNVNIGDKFLIYAPLQAVKHPRTHELYGRLIRGLG
                     ILQITAKDPAADVVTARITLSFDSIAAGSLLAPYQEPSFVFESAEKKAKDISGYILEV
                     TDHRTINGQTDVVYLDKGKTDGVDPGDKFIVYADPARRGYPREKVGEVQVFLVKERTS
                     TAMVRKSIDVLQKGQAVDFKK"
BASE COUNT          690 a          968 c          939 g          623 t
ORIGIN      
        1 cgaacacggc gtcgaaatcc ggggctccct cagttttctt tttccgcaga cgctcggccg
       61 aaagcttgat cggcgtcaga gggtttttga actcgtgggc tatcctccgg gcaacatcct
      121 gccaggtctc ggcctttttc gcccgtaaaa gctccgtcat gtcgtcaaag gtgacgacgg
      181 acccgatcgg agagccgccg ccgtcccgca gcgtcgtgat gcgcagccgg atggtcagca
      241 cgcggccgcg cacagtgagc gacagctcct tctcggcgct tccctcgccg ggttcgagct
      301 tgcgaaagag gccgcggatc gggtccagct cgatgaaccc gaaggctgca tcgtaccgct
      361 tgcccagcac gtccgcttcc cggatgccga ggatctccga cgccgactgg ttgacggtcg
      421 tgatcctccc ggcgcggtcg atggatacga cgcccgtgtc gatattcgac aggatggtct
      481 tcttgtaatt gatttcctgt tcgagctgct cccgggagct cttgagctcc cgcgtcatca
      541 cgttgaagga gtcaacgagt acgccgacct cgtcgcccga tttctcgtcg atctggtagt
      601 cgaattttcc cgcggccacc gccgcggtgc cctccgccag cttcctgatc ggcacggtga
      661 tgccggccgc cacccggaga gccacccaga tcgcggaaag aaggagcgca agggttacgg
      721 cgagaaaccc gagccggtag gcaagcttga tgggttcttt gctcttaaac gccgaacggt
      781 attgcgcata gccttcatgg atttcttccg ctttcgccgc caggttcggc gggatataat
      841 aactcacggc gaccgccgcc acgaccttgt tcccttccgg agtttcgtgg acgggaacga
      901 ccacccgcat gatgtcgccc ttcttcgcgg tacccgacag gtcggcggcg tccaacgcgg
      961 cgaatgccag cgcaaccagt tccgggggcg tctggctaaa tgcctgcggg gggaactgct
     1021 ctgtaacgat gaccgccttg cgcctttgcg gcgcggtaaa gagctccacc gaacccaggc
     1081 cgtattcctt cgcctttttc gccagatatt ccgtgatcac cacattgtcg aaacgggaca
     1141 agagccttcc ctcggtcatc tggaggctga tctgcttccc gaaaaccagg gcatctttcc
     1201 ggagccggcc gtaatattcc tgggcgagtg ataccgagtc cttcagcgaa ttctcgatgc
     1261 gcaggctgaa cagccgttcg atgctggtgg tgaacatgcc gctcccgatg atgaaaagga
     1321 gaatgggagg gatgaacgag aggccgacaa aggcaaaaac cagttttgtc ctgaaccggg
     1381 ctcccaatac gccgtgcttt cgctcgatcg agagcttcca gaggcttctg gctatcatga
     1441 agacgacggc agcaaggagc aggaagtttg cgttgatgag aaagagaatg aggaggttgt
     1501 ctgacagggg cccttcgaaa ccccggtact ggagctggag cacgattgcg agcgccgtca
     1561 cgaccagaaa accaaagagg tacagcaccc ccttccatcc tgtttggcct tctttgacgt
     1621 tggtcgccat ggtattcgca gaataccaaa aaccccttgt aaaagcaata cttggtttgt
     1681 gcacaccctg atttattctc ttgacaaaca agaggtttct cctatatctt aaggaaaaat
     1741 gacacccggg atacgggcaa gggtaacgat gcccgtttcg caaagcgtct cacggttctg
     1801 tcttcatgac caacgactcc ggacgcagcc gaacgggctt tgctgccgtt cttcattttt
     1861 ccgtattcat ctccgtccat cggcgtagat ctttttccag gaggcaggaa tgaccataaa
     1921 gacgaacgtc ttgatccagg cagcatgcgt ttctctgctc cttgcgcttc ttccgggaac
     1981 cgctgccccc caggagccga aagagaccgg gcagcagccc gcgcaagcgt ccggcggacc
     2041 ggaacaactg aagtcggagg ccgtgacaac gccatccgca ggcgccgccg agccgcaacc
     2101 caagcaggaa gcggcgcccg ccattaaggc ggaagaggcc gctcctgccg ccgagaaaga
     2161 gctcctgtat acgatcaagc ggggtgatac gctctgggac atttcaaaca cctttttcaa
     2221 agacccattt ctgtggccct tcatctggaa ggcaaacccg ccgataacca accctgacct
     2281 gatttatgcc ggcaacaaac ttgtcatccc gagccttgcg cctattgagc gggcgatcaa
     2341 ggcccctgcc gcggccgccc cgacggagga gcaggccccg ggcccggaaa ccgccgctgc
     2401 cccacacgcg ctgccgaaac cgcccgccgg cgtaacggaa gaggcgcccg ccgggggcgg
     2461 gaagatcatc gtgcccgagg aagaggcgca gcccgtggtc gataagtact ccttcctgag
     2521 tgcgggcttc gtgaatgacg cggagagcgt gggaacgatc atcggcccgg ctgttcagga
     2581 aaagacgatc atgggatatg acgacgttgt ctacgtcagg tttggagccg acaataacgt
     2641 gaatatcggc gataaatttc tgatctacgc tccgctccag gccgtgaagc atccgaggac
     2701 gcacgaactg tatggcaggc tcatacgcgg cctgggcata cttcagatca cggccaagga
     2761 ccccgccgcc gacgtggtca cggcgcggat tacactctcc ttcgactcca tcgcagcggg
     2821 gagcctgttg gccccctacc aggagccgtc gttcgttttt gagtccgcgg aaaagaaagc
     2881 caaggacatc tcggggtaca tcctggaagt gaccgaccac cgcaccatca acggacagac
     2941 ggacgtcgta tacctggaca aggggaaaac cgacggggtc gatcccggcg acaaattcat
     3001 cgtctacgcg gaccctgcca ggaggggata cccgagggag aaggtcggcg aagtccaggt
     3061 gtttctcgtg aaggaacgca catcgacggc catggtgcgc aagagcatcg acgtcctgca
     3121 gaaaggccag gcggtagact ttaagaaatg aagaaaaaat gttgcgggtt gcgcgttgcg
     3181 gggtacgggt taaaacccgc aacgcataac tcgcaactcg
//
LOCUS       DASSLD010000327         2550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1366673, whole genome shotgun sequence.
ACCESSION   DASSLD010000327 DASSLD010000000
VERSION     DASSLD010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366673"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..702)
                     /gene="hisH"
                     /locus_tag="VFK23_05930"
     CDS             complement(64..702)
                     /gene="hisH"
                     /locus_tag="VFK23_05930"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HET7318657.1"
                     /translation="MIAIIDYGMGNLRSVQKGFERMGHEAVVTNDAKTILSASKVVLP
                     GVGAFPDCMRNLREYGLIDAVYKSIDSGKPFLGICLGLQLLFTESEEFGISKGLDIIK
                     GRVVRFKGPAFDNQQSTIDNLKVPHMGWNSVSIKRRPLALQDVPEGSHVYFVHSFHVV
                     PEDADVIATTTDYGFEFVSSIWKDNLFASQFHPEKSQTVGLSILKRFGEQKA"
     gene            complement(835..1425)
                     /gene="hisB"
                     /locus_tag="VFK23_05935"
     CDS             complement(835..1425)
                     /gene="hisB"
                     /locus_tag="VFK23_05935"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HET7318658.1"
                     /translation="MPRKATIERKTKETDIRISLDLDGSGRYNIKTSVPFMDHMFSHV
                     AKHGFFDLTIKAKGDTEIDDHHTVEDLGIAFGQALKKALGDSHGIGRYGDVTVPMDET
                     LAQVVMDLSGRPYLAYHVELPKKYKLKDFDPGLVEDFFQSVVNHAGMNLHIRLHYGRD
                     VHHMLEAVFKAFGRALDQATSIDPRIKGVLSTKGKI"
     gene            complement(1505..>2550)
                     /gene="hisC"
                     /locus_tag="VFK23_05940"
     CDS             complement(1505..>2550)
                     /gene="hisC"
                     /locus_tag="VFK23_05940"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET7318659.1"
                     /translation="LIRPQVRKLTAYHVDETPVRIKLDAMENPFLLPDAIRREIAGAV
                     KNARINLYPDPSAKAVKKAIAAMWGVKPGQMVLGNGSDELIQAVILAFGGPVLIPVPT
                     FAMYEITARALGQEVVTAPLGKDFDLDADLVINKARKSKAKIVFLACPNNPTGNRFDD
                     RAVRKVLDNVNAAVVLDEAYYSFSGKTYLPLLKKYPNMIILRTLSKVGFAGLRVGLLT
                     AAPAVVEELSKVRLPYNINMLSQTAAVTALRHRDVIERQISVLISEREKLYNALSRMG
                     GVTAYPSETNFILIRTARDAARIHGRLKQSGILVKNLDRPGPLKNCLRVTVGKPAENR
                     EFLRTLERILTAN"
BASE COUNT          499 a          715 c          743 g          593 t
ORIGIN      
        1 catatccgtg tcaataaaga ttttgacttt ctctgtgtcc tctgtgatct ctgtggcaaa
       61 aaatcaagcc ttttgttctc cgaaccgctt caggatcgac agccccaccg tctggctctt
      121 ctccggatgg aactgcgatg cgaagaggtt gtctttccag atgctggaga cgaactcgaa
      181 cccgtagtcg gttgtcgtcg cgatcacgtc cgcatcctcg ggcaccacgt ggaaggaatg
      241 aacaaagtag acgtggctgc cctccggaac gtcttgaagg gccaggggac ggcgtttgat
      301 cgagacagaa ttccatccca tgtgaggaac cttgagattg tcgattgtcg attgttgatt
      361 gtcgaaggcg ggccctttga acctgaccac cctgcccttg atgatgtcga ggcccttcga
      421 aattccgaac tcctcgcttt ccgtgaagag gagctgaagg ccgaggcaga ttcccaaaaa
      481 gggcttgcct gagtcgatcg atttatacac tgcgtcgatg aggccatact cccgaaggtt
      541 tcgcatgcag tccgggaagg cccccacacc gggaagaacg accttcgagg cgctcagaat
      601 cgtcttggcg tcgttcgtga ccacggcctc atggcccatg cgctcgaagc ccttctggac
      661 gctccgcaga tttcccattc cgtaatcaat aatcgcgatc ataatatttc tagatttctg
      721 tccacaaggt gtcaccctga gcggagcgaa ggggctggct ccggattgaa aagcgagatt
      781 cttcgctgcg ctcaggatga caaccggatt gcctcgtcat tcgtacaact gtttttaaat
      841 tttgcctttc gtcgaaagta cgcccttgat cctggggtcg atgcttgtag cctggtccag
      901 ggcgcggccg aaggccttga agacggcttc aagcatgtgg tgaacgtcgc ggccgtagtg
      961 gaggcggatg tgcaggttca tccccgcatg attcacgacg gactggaaga agtcttccac
     1021 cagtccggga tcgaaatcct tcagcttgta tttcttcggc agctccacgt ggtatgcgag
     1081 gtacggcctg ccggacagat ccatcacgac ctgtgcgagg gtctcgtcca tgggcaccgt
     1141 tacatcgccg tagcggccga tgccgtgact atctcccagg gcctttttca gcgcctggcc
     1201 gaaggcgatg cccaggtcct cgaccgtatg atggtcgtcg atctccgtgt ctcccttcgc
     1261 cttgatcgta aggtcgaaga agccgtgctt cgccacgtgg gagaacatgt ggtccatgaa
     1321 cgggaccgag gtcttgatgt tgtatcggcc ggagccgtcg aggtccagcg aaatccttat
     1381 gtccgtttcc ttcgtctttc gttcgatagt tgctttgcgc ggcatggaga ctcctcacgt
     1441 ggatgcaaga tgcaggataa atgattcatg gttcaagcat gcatcaggga tcatgcatca
     1501 tggatcaatt cgctgtcaga attcgttcca gtgtcctcaa aaactcccgg ttctccgcag
     1561 gcttgcccac ggtcacgcga aggcagtttt tgagaggtcc gggccggtcc aggttcttga
     1621 ccagaatgcc cgattgtttc agccttccgt gaatgcgcgc tgcgtctcgt gccgtcctga
     1681 tgaggatgaa gttggtctcc gagggatagg cagtgacgcc ccccatgcgc gagagcgcat
     1741 tatagagctt ttcccgctct gaaatcaaca cggaaatctg gcgttcgatc acgtcgcggt
     1801 ggcgcagggc ggtcactgcc gcggtctgcg agagcatgtt tatgttgtac ggcagccgga
     1861 ccttggagag ctcttccacc accgccgggg cggccgtgag caggccgacc ctgagcccgg
     1921 caaagccgac cttcgagagc gtacggagga tgatcatgtt cgggtatttc ttcaggagcg
     1981 gaagatacgt cttcccggaa aagctgtagt aggcctcgtc cagcaccacg gctgcgttca
     2041 cgttgtccag gaccttccgc acggcgcgat cgtcaaagcg gttgccggtg ggattattgg
     2101 gacaggcgag aaagacgatc tttgctttgc tctttctcgc cttgttgatc acgaggtccg
     2161 cgtcgaggtc aaaatccttc ccgagaggcg cggtcacgac ttcctggccc agcgcccgcg
     2221 ccgttatctc gtacatggca aaggtcggaa ccgggatgag cacgggtccg ccaaaggcga
     2281 ggatgacggc ctggatgagc tcgtcggacc cgttgccgag aaccatctgc ccgggcttca
     2341 ccccccacat tgctgcaatg gccttcttca ccgcttttgc ggacggatcg gggtagaggt
     2401 tgatgcgggc attcttcaca gcgccggcga tctcccgccg aatggcatcg ggcaggagga
     2461 aggggttctc catcgcgtcg agcttgatcc gtacaggtgt ttcatcgaca tgataggccg
     2521 tgagctttcg cacctgcggc ctgatgagcc
//
LOCUS       DASSLD010000328         3434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1044370, whole genome shotgun sequence.
ACCESSION   DASSLD010000328 DASSLD010000000
VERSION     DASSLD010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044370"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VFK23_05945"
     CDS             complement(<1..534)
                     /locus_tag="VFK23_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET7318660.1"
                     /translation="MNSRERVLKMFAGEEVDRPPCFSGMGNVTTEGLKSLNQKFAATH
                     LDAKMMAAAAATTYKLFGFECAVAPFDLCIEAEAMGCEINVYAHSEDLLYPTIKKKLI
                     HNEAEMELAIPANLTARGRVPLMVETIKLLKKDIGSEAAVGSYVLGPFTLAGQVMELN
                     DLLKLSFKKPDKVSKLLE"
     gene            complement(671..1618)
                     /locus_tag="VFK23_05950"
     CDS             complement(671..1618)
                     /locus_tag="VFK23_05950"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit H"
                     /protein_id="HET7318661.1"
                     /translation="MFQFNKDQKVFTMAGMKVGGQPGENPPLQIASMFHNKDRIVTDR
                     KGNFDRVKAAELIKTMEQLSASTGIPAMVAMVANSAEEAKIYVDFYRETTTMPFGIDM
                     WVAEKRAQATEYVAKLGLQDKFLYNSITPWDKDIKGQVQKLKDLGIRHVVVQAFDDQD
                     QSPAGRLKSLENILNQGADSFETIIVDTSVMNLPATSFSLVANRMIKEKLGLPCGGAY
                     SNGTHMWKEAKTIWSLDGFKAMDAVVQGMASVLWSDFNFCGPIVTMPRIFPAVAAAHV
                     LLTTLVYDETKKIPENANIPIKKYFGDFLQKLTSGAARK"
     gene            complement(1712..2155)
                     /locus_tag="VFK23_05955"
     CDS             complement(1712..2155)
                     /locus_tag="VFK23_05955"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7318662.1"
                     /translation="MADKKMTLEEVNEYMKKQCGFVPRMFQIINTVTPEPGRTFADFY
                     ASIFGDGALSRKHKELMFMSGGVAYCSPRCIIHVIPAINAGATTGEIFEAASVGMILG
                     GFVPGGPGIPYAFEYALKCLDIEAKYRKGEKWEYLPAPRFDHGVF"
     gene            complement(2274..3311)
                     /locus_tag="VFK23_05960"
     CDS             complement(2274..3311)
                     /locus_tag="VFK23_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET7318663.1"
                     /translation="MKPKEVVLKAFEGGKGDRIPVTLFGAGMWSIKDFGTTFEALAHD
                     PQKMTEMCVAEAARIRSDVVYVGSGFNNFHASALGKAFGVGIKYREMGAPDMTTHFVN
                     SEEDLAKLDIADVHKEPVVQTVLEATRRVREKIGDTYLVTMTCWGPFTLAARFVGEEA
                     FMKATFKKPAFVEKMVDFCANLLIHLYEPLVKDGLECLSIADPTASGDLINKKQMERF
                     AVPGLKKLTGWAKGKGAHSILHICGNTSDRLDLFPMTGASTIFFDQKVDIAKAKEALH
                     GKMSFGGNIAPVHVLLNQDVAGVEKACREVIEIAGKDGGFILVPGCDIPPTIPYENLK
                     KFHDVALEWKL"
BASE COUNT          645 a         1047 c          967 g          775 t
ORIGIN      
        1 ctcgagcagt ttcgaaacct tgtcgggctt cttgaaggac agtttcagga ggtcgttcag
       61 ctccatgacc tggcccgcga gcgtgaaggg ccccagcaca taggacccga cggctgcctc
      121 ggagccgatg tccttcttga gcagcttgat cgtctccacc atgagcggca ccctgccccg
      181 cgccgtgaga ttcgccggga tggcgagctc catctccgct tcgttgtgga tcagtttctt
      241 cttgatcgtg ggatacagca ggtcctctga atgggcgtac acgttgatct cgcatcccat
      301 ggcctcggcc tcgatgcaga ggtcgaaggg agcaaccgcg cattcgaacc cgaacagctt
      361 gtacgtcgtg gccgccgccg cggccatcat cttggcatca aggtgcgtgg ccgcgaattt
      421 ttgattgagc gatttcagcc cctcggtggt cacgttgccc atgccggaaa aacacggcgg
      481 ccggtccacc tcctcaccgg cgaacatctt cagcacccgt tctcgtgaat tcatcgttaa
      541 tcgatctcct tccctcaatt catagttaac cgcgaagacg cggggtcgca gaataaattt
      601 ccataacgga taaacaaaag aggattagaa aaaattcgtt gctccgcgcc ttctgcggtg
      661 gctgtttcat ttattttctc gccgcccccg aggtcagctt ttgaagaaaa tccccgaagt
      721 atttcttgat cgggatgttc gcattctcgg ggattttctt tgtctcgtcg tacacaaggg
      781 tcgtgagcag gacgtgcgcg gcggccactg ccgggaagat gcgcggcatc gtcacgatgg
      841 ggccgcagaa attgaagtcg ctccacagca ccgaggccat tccctggacg acggcgtcca
      901 tggccttgaa cccgtcgagg ctccagatcg tcttcgcctc tttccacatg tgcgtgccgt
      961 ttgaataagc gccgccgcag ggaagcccga gcttctcctt gatcattctg ttggcgacga
     1021 gcgagaacga cgtggcgggc aggttcatga ccgaggtgtc cacgatgatc gtctcgaagc
     1081 tgtcggcgcc ctgattaagg atgttctcca gggacttgag ccggccggcg ggcgactggt
     1141 cctggtcgtc aaaggcctga acaacgacgt gcctgatccc gaggtccttg agcttctgca
     1201 cctggccctt gatgtcctta tcccacgggg tgatgctgtt gtagaggaac ttgtcctgca
     1261 gacccagctt ggcgacatat tccgtggcct gcgctcgctt ttccgcgacc cacatgtcga
     1321 tgccgaaggg catcgtcgtc gtctcgcggt aaaagtctac gtagatcttt gcctcttcgg
     1381 ctgagttggc caccatggcg accatggcgg gaatgccggt ggaggcggac agttgctcca
     1441 tcgttttgat gagttccgcg gccttgacgc ggtcgaagtt ccccttccgg tcggttacaa
     1501 tccggtcctt gttgtgaaac atggatgcga tctggagcgg cgggttttca cccggctgtc
     1561 cgccgacctt cataccggcc atggtaaata ccttctggtc cttattaaat tggaacatat
     1621 gaacctcgat tcgttttcta aatttataat tatagaattg ataagtccaa aaaacagggg
     1681 agctttcgaa acgctccccc gcgaatcgaa tttagaacac cccgtggtcg aacctgggcg
     1741 cgggaaggta ctcccacttc tcacccttgc ggtatttggc ttcgatgtcg aggcacttga
     1801 gagcgtactc gaaggcgtag ggaatgccgg gcccgccggg cacaaagccg ccgaggatca
     1861 tgccgaccga cgccgcctcg aagatttcgc ccgtggtcgc gcccgcgttg atcgccggga
     1921 tcacgtggat gatgcatcgc ggagaacaat acgcgacgcc gccggacatg aacataagct
     1981 ccttgtgctt gcgggaaagg gccccgtcac cgaagatgga agcgtagaaa tctgcgaagg
     2041 tccttccagg ctcgggcgtc acggtgttga tgatctggaa catcctcggc acgaacccgc
     2101 actgcttctt catgtactcg ttcacttctt ccaacgtcat tttcttgtca gccatttttc
     2161 cctccccttg gccggacgcg ctctgcgccc gggaaaacag tttcttcaac tcctccaaaa
     2221 tacccacgaa ttcgacctcc tttcacgaaa ccgaaccgca tcacctcctt ctgtcagagt
     2281 ttccactcaa gcgccacgtc gtggaacttt ttgagattct cgtagggaat cgtcggcggg
     2341 atgtcgcacc cgggcacgag gatgaacccg ccgtccttgc cggcgatctc gatgacctcg
     2401 cggcaggcct tctccacccc cgcgacgtcc tggttcaaga gcacgtgcac cggcgcgatg
     2461 ttgcctccga agctcatctt cccgtgcagc gcctccttgg ccttggcgat gtccaccttc
     2521 tggtcgaaga agatcgtgct cgcccccgtc atcgggaaca gatccagccg gtcgctggtg
     2581 ttcccgcaga tgtgcaggat cgagtgcgcg cccttgccct tggcccagcc ggtgagcttc
     2641 ttcagacccg gcaccgcgaa gcgctccatc tgcttcttgt tgatcagatc tcccgaggcg
     2701 gtcgggtcgg cgatgctgag gcactcgagg ccgtccttga ccagcggctc gtagaggtgg
     2761 atcagcaggt tggcgcagaa atcgaccatc ttctcgacaa acgccggctt cttgaaggtc
     2821 gccttcatga acgcctcctc gccgacgaag cgcgccgcaa gggtgaacgg cccccagcag
     2881 gtcatcgtca cgaggtaggt gtcgccgatc ttctcccgga cgcgccgggt cgcctcgagc
     2941 accgtctgga cgaccggctc cttgtgcacg tcggcgatgt cgagcttcgc caggtcctcc
     3001 tcggagttga cgaagtgggt ggtcatgtcg ggagcgccca tctcgcggta cttgatcccg
     3061 accccgaagg ccttgccgag cgcagaggcg tggaagttgt tgaagcccga gccgacgtag
     3121 acgacgtcgc tgcggattcg cgccgcctcc gccacgcaca tctcggtcat cttctgcgga
     3181 tcgtgggcga gtgcctcgaa cgtcgtcccg aagtccttga tcgaccacat acccgcgccg
     3241 aagagggtca cgggaatccg gtcgcccttg ccgccctcaa atgccttgag taccacttcc
     3301 ttcggtttca tagcatctcc cttctcgaat aaagtggaat caaaccttca gtctgccggg
     3361 gcattcatca tgtattgtta cgagtgttcg ggctgacaga aacggcttaa gcttcagact
     3421 gcaatgctgt ggat
//
LOCUS       DASSLD010000329         3482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1043555, whole genome shotgun sequence.
ACCESSION   DASSLD010000329 DASSLD010000000
VERSION     DASSLD010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043555"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2565
                     /locus_tag="VFK23_05965"
     CDS             <1..2565
                     /locus_tag="VFK23_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7318664.1"
                     /translation="PQQPDLAYGQFIVNTASVPATARLVPELRASLATLAPEAIVIVK
                     ELQQGSQIEAPVEIRISGDDIAELKRLGGQLREILQEISFSQFVHDDYYNDSYMTDLN
                     VNNEMANRLGITDASVAGMLAGAFDGAPVSTFWEGDRAVGIKLRLDQRARENFADVGN
                     TYVTSQLTHASVPLRSFATLAPEWQTSRIVRRNGVRTLTVRSFVKQGHYASQLLKQAA
                     PKLAAVKLPPGYRMEYGGDRFNRDETFPQMLGALAISLVAIFLVLLIQFKRITDPLVV
                     MSSIPLMLFGAVVGLIVTRNTMGFTAFLGLISLCGIVVRNSIILIDYMNERLGHGCSL
                     EEAAEEAGKRRMRPIFLTTMAAAVGVTPMILSGSSLWSPLASVIAFGLIFSMCFTLLV
                     VPVLFVVVRSRTFKPAAVVLVLAAFALLGAAGGASAESAKAPLTLNEAVETALNRNKA
                     LRIGRAKVVENERKVDASRADYFPVLSNSSKALHLSNTQLMTIPAGSLGSLGGSPFPD
                     KSVSIDQGKSSFLVSETTLSQPLTQLLKIGAAVDVARADRGIAEADLKKSENEIVLAV
                     HQLYYGLLIASKEREAAQAMLAAARDDLRETEEGVRSGNLLDVATNAGRVKLLQSRQA
                     LIAAENRIADFSSELNDLLGFPLDAPVEVTEAGLQDLPLPPREESFRTARPNNPELAA
                     ARETVEKSRSAVRAARYEYIPDLTLFASHLYQDGAPFLTQNVEVIGAQFSWKIFDWGK
                     RNAQIRERKAQLLQAEENLARQDDRVTVEIDKVYRKLERTKRMVEVAREAVALSRENV
                     RLSENRVKAEAVTDARHSEAVAAMKKAEMEELQASLDYRLTRMELDRITGTLASGR"
     gene            2603..3226
                     /locus_tag="VFK23_05970"
     CDS             2603..3226
                     /locus_tag="VFK23_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7318665.1"
                     /translation="MALSNFNKNPLGNPEQNGNAVRDRLLDAALRLFARKGFESASVR
                     EIVKAAEVTRPTLYYYFGSKEGLYLELVERLCATVEDSILHSMVPQGTLQDRLRSFVL
                     NILDSIIEDTDNKRFFFVISLDPRRNILSSFHERMRNFIAAVVELLLEEGVEKGEFET
                     DDVRCITKLILALVDSFIYNQIFLTNPKSNRDETEKMLDLLLDQIAL"
     gene            3269..>3482
                     /locus_tag="VFK23_05975"
     CDS             3269..>3482
                     /locus_tag="VFK23_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7318666.1"
                     /translation="MTPDEDRRKFSEGGPGLPLGAFLAGPFAFFGRSAILWINSSGAA
                     VIFFLLAFQKIFRPKQLSKIVQQVYYI"
BASE COUNT          703 a         1050 c         1073 g          656 t
ORIGIN      
        1 ccccagcagc ccgacctggc ctatggccag ttcatcgtga acaccgcctc ggtcccggct
       61 accgcccggc tggtccccga actgcgggcg agccttgcga cgctcgcgcc cgaagcgatc
      121 gtgatcgtga aggagctgca acaagggtcg cagatcgagg ctccggtgga aatccgcatc
      181 tccggcgacg atatcgcgga gctcaagcgc ctgggggggc agctccggga gatcctgcag
      241 gagatttcct tctcccagtt cgtgcacgac gattactaca acgactccta catgacggat
      301 ctgaacgtga acaacgagat ggccaaccgc ctgggaatca ccgacgcgtc ggtggcgggg
      361 atgcttgccg gggccttcga cggcgcgccg gtcagcacct tctgggaagg cgaccgcgcg
      421 gtcggcatta agcttcggct cgaccagagg gcgcgggaaa acttcgccga cgtcgggaac
      481 acctacgtga cgtcgcagct gacgcacgcc agcgtaccgc tgcgctcctt cgccaccctt
      541 gcgccggagt ggcagaccag ccggatcgtc cggagaaacg gggtgcggac cttgaccgtg
      601 cgcagtttcg tcaagcaggg gcattacgca tcgcaactcc tgaaacaggc ggctccgaag
      661 cttgccgccg tcaagctccc tcccggctac cggatggaat acggggggga caggttcaac
      721 cgggacgaga cctttccgca gatgctcggg gcgctcgcga tcagcctcgt ggccatcttc
      781 ctggtcctgc tgatccagtt caagcggata acggatccgc tcgtggtcat gtcgtcgatc
      841 ccgttgatgt tattcggggc ggtcgtgggg ctgatcgtga cccgcaacac gatggggttc
      901 accgccttcc tggggctgat cagcctgtgc ggcatcgtcg tacggaacag catcatcctg
      961 atcgattaca tgaacgagcg gcttggccac ggatgctcgc tcgaggaggc ggccgaggag
     1021 gcgggcaaac ggcgcatgcg gccgatcttt ctgaccacca tggcggcggc ggtgggggtg
     1081 accccgatga tcctctccgg ctcaagcctg tggagtccgc tggcgagcgt catcgccttc
     1141 ggcctcatct tctcgatgtg cttcacgctg ctggtcgtcc cggtgctgtt cgtggtggtg
     1201 cggtcccgta ccttcaagcc ggctgcggtc gtcctcgtcc tcgcggcgtt cgccctgctc
     1261 ggggcggcgg gcggcgcctc ggccgagagc gcgaaggctc ccctcaccct gaacgaggcg
     1321 gtcgaaacgg cgctgaatcg caacaaggcg ctccggatcg ggcgggcgaa agtggtggaa
     1381 aacgagcgca aggtagacgc gtcccgcgcc gactatttcc ccgtcctttc caacagctcc
     1441 aaggcccttc acttgagcaa cacccagctc atgaccatcc ccgctggcag cctgggatcc
     1501 ctcggcggca gcccgtttcc ggacaagtcg gtttccatcg atcagggcaa gtccagtttc
     1561 ctggtcagcg aaacgacgct gagccagccg ctgacccaac tcctcaagat aggcgcggcc
     1621 gtcgacgtcg cccgggccga tcgcggcatc gccgaggcgg atttgaagaa gtctgaaaac
     1681 gagatcgtcc tcgcggtgca ccagctctat tacgggttgc tgatcgcctc aaaggaaagg
     1741 gaagccgccc aggccatgct ggcggctgcc cgagacgacc tgcgcgagac cgaggaagga
     1801 gtccggtccg gcaaccttct cgacgtcgca acgaacgcag gccgggtcaa gttgctccag
     1861 tcccggcagg cgctgatcgc ggccgaaaac cggatcgcgg acttcagctc cgaactcaac
     1921 gacctgctgg gctttccgct cgacgctcct gtggaggtga ccgaagccgg ccttcaggac
     1981 cttccgctcc ccccccggga ggaatcgttc cggacagcgc gaccgaacaa cccggaactg
     2041 gcagcggccc gcgagacggt ggagaaatcg cgctccgcgg tccgtgccgc gcgttacgag
     2101 tacatcccgg acctgactct tttcgccagc catctctacc aggacggcgc gccgtttctt
     2161 acccagaacg tggaggtcat cggggcgcag ttcagctgga agatcttcga ctgggggaaa
     2221 cggaacgcgc agatcagaga gaggaaggcg caactcctcc aggcggaaga aaacctggcg
     2281 cggcaggacg accgggtgac ggtggaaatc gacaaggtat accggaagct ggagaggacg
     2341 aagaggatgg tggaggtcgc ccgcgaggcg gtcgccctgt cccgggagaa cgttcgcctg
     2401 agcgagaacc gcgtgaaggc cgaggcggtc accgacgcaa ggcattccga agcggtcgct
     2461 gccatgaaga aggcggagat ggaggaactc caggcttccc tagattaccg gctgacccgg
     2521 atggaactcg accggatcac gggaacgctc gcgtccggcc gctagagatc ccggcatcct
     2581 atttcccaat ttagagagga cgatggcact gtcaaacttc aacaagaacc ccctcgggaa
     2641 cccggaacag aacgggaatg ccgttcggga tcggctactg gatgcggcgc tgcgactgtt
     2701 tgcccgaaag ggcttcgaat cggcgagcgt acgcgagatc gtcaaggccg cggaagtgac
     2761 ccggccgaca ctctattact atttcgggag caaggaaggg ctctatctcg aactggtgga
     2821 acggctgtgc gcaaccgtcg aggattcgat cctccattcc atggttccgc agggaacctt
     2881 acaggatcga ctgagatcgt tcgtgctgaa tatcctggac tccatcatcg aggataccga
     2941 taacaagcga ttcttcttcg tcatcagtct ggaccctcgc cgaaatatcc tgtcttcctt
     3001 ccatgaacga atgagaaatt tcatcgccgc cgttgtggaa ttgctgttgg aggaaggagt
     3061 cgaaaagggg gaattcgaga ctgacgacgt tagatgtatc acaaaattga tattggcgct
     3121 tgttgattcc tttatatata accagatttt ccttactaac ccgaagtcaa atcgggatga
     3181 aacagaaaag atgttggacc ttcttctcga ccaaatcgca ttgtagttca ttttccatgt
     3241 tgttacgtga caattatggg gccctttcat gacgcctgat gaagacagac gaaagttctc
     3301 tgaaggtgga ccgggactgc cgcttggcgc attcctggca ggtccatttg cctttttcgg
     3361 aaggtcggcg atcctctgga tcaacagttc aggcgccgca gtaatttttt tcctgctggc
     3421 atttcagaag atattccggc ccaagcagtt atccaagatc gtccagcagg tctattacat
     3481 cg
//
LOCUS       DASSLD010000330         3588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_187863,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000330 DASSLD010000000
VERSION     DASSLD010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187863"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFK23_05980"
     CDS             <1..378
                     /locus_tag="VFK23_05980"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET7318667.1"
                     /translation="NKYVLPVSFNNKWRGSMTNYLEQRKSVRFNGEIPIELKQGTGMT
                     RNCSADGIYFVTDQSVSVGERIELIMFLDHIGLGHTVRLRCTGEVLRLKSEAGLNGVA
                     FAFTSQVLDATNMMLNTKKEEVR"
     gene            375..986
                     /locus_tag="VFK23_05985"
     CDS             375..986
                     /locus_tag="VFK23_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318668.1"
                     /translation="MKARVTRIYAAIMALAALIMLPLAALANPAVVSITAPVDGSTTY
                     VDAFPTGVTVSGTVVHSPGTVNDMRACVTVDNDPPVCGNYVSGVGNTSSFPFAISVVI
                     YSAGTHSFVVSADKSSGGHYGFAQVTATYLLSATICDEKDPPAIANEYLDSKDTPDAF
                     KTIRGKIISVIAQNMNEGRYGSCNYNVTLVQSDVDALLVKLLP"
     gene            complement(1090..1728)
                     /locus_tag="VFK23_05990"
     CDS             complement(1090..1728)
                     /locus_tag="VFK23_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7318669.1"
                     /translation="MSIRLVLADDHPIILNGLENLFRLEEGFDVLASCADGVGAMQAV
                     RQFRPDVAVLDISMPGMSGLEVARKIREEKLPTRVVLLTAALKDEEMLDAVHLEVNGI
                     VLKEMAPQFLVQCIRKVHAGEQWIERRSAKQVLEKMLRRETGEREAAAILTPREIELV
                     RMVARGLRNKDVADKLCISEGTVKVHLHNIYEKLNVDGRMALLRYAQEKGLV"
     gene            complement(1730..3457)
                     /locus_tag="VFK23_05995"
     CDS             complement(1730..3457)
                     /locus_tag="VFK23_05995"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET7318670.1"
                     /translation="MEHSTASLHAGKQYVPQRRAERLIATGRMILSAFFLLAIWIDPS
                     EPSRYARLTYAILAGYLIYSLLLGVMTLKQYFNKGRLHVVTHAIDMLVFALLMFLTEG
                     STSPFFVYFIFLLVCATLRWQRRGTLWTAVTALAVVIFLAWFPSNLLHDQNFELNRFI
                     IRVVYLAVVAVLLGYLGEYEQSMSSVFSKLAGWPRSVSDDPQSLSCEILGHAATILNA
                     PRVVLAWEEAEEPWLHLASWSRDGCQYRREPPAVFGTLVAEQLSGTSFFCQNAGDPQS
                     PVIHSDRAGLRRWQGTPLDPELQKNFAVRAILASRFTGEKAAGYLLSLDKTPMTADDL
                     VLGGIVANEVAGRLDRCYLVKQQQEAAASNERIRLARDLHDGLLQSLTGAALQLETAH
                     RLMETEPQTAGQRIREIQRLLASEQRDLRSHINELRPFPQGRPARDFELTSRLEELAE
                     RIRRQWDLSVEITTAPAAPRIAMSMAREVYFVVHEALINAVRHAGASHLRAGLAFKAD
                     RVHITVADDGHGFSFRGNYDQDALFAMKRGPVTLKERIAALRGTLAIESREDGARLEI
                     ILPLTERGG"
BASE COUNT          795 a         1042 c          999 g          752 t
ORIGIN      
        1 aataaatacg ttcttcctgt atcgtttaac aacaaatgga ggggttctat gacaaattat
       61 ctcgagcagc ggaaatccgt acgttttaat ggtgaaatcc ctatagaact gaaacagggt
      121 acgggcatga cccgcaattg cagcgctgac gggatatatt tcgtgacaga ccagtccgtg
      181 tccgtgggtg aacgaataga gcttatcatg tttctggacc acataggttt gggacataca
      241 gtacggctcc gctgcacggg agaggtcctg aggttgaagt ccgaagcagg gttgaacgga
      301 gtcgcctttg cctttacttc ccaggtgctc gatgcgacaa atatgatgtt aaatacaaaa
      361 aaggaggaag tacgatgaaa gcaagagtga caagaatcta tgcagcaatc atggcgctgg
      421 cggcgctgat aatgctcccg ctggcggcac tggcaaatcc cgccgtcgtt tccattacag
      481 cgccagtcga cggctctacc acctatgtcg atgcctttcc gacgggcgtc acggtttccg
      541 gaaccgttgt gcattctcca ggaactgtca acgacatgag ggcttgtgta actgtcgata
      601 atgacccgcc tgtctgcggg aattacgttt ccggcgtcgg caatacgagt tccttcccgt
      661 ttgccatctc tgtggtgatc tacagtgccg gcacccattc cttcgtcgtc tctgccgaca
      721 agagcagcgg cggtcattat ggttttgcgc aggttacggc aacgtacttg ctctccgcaa
      781 ccatctgtga cgagaaagac ccgcctgcca tcgccaatga gtaccttgac agcaaagata
      841 ctccggacgc ctttaaaacg atcaggggga aaattatcag cgtcattgca cagaatatga
      901 atgaggggag atatggttcc tgcaactaca acgtcactct tgtgcagtct gacgtcgatg
      961 ctctgcttgt caagctgctg ccgtaaccct ggttgaaaaa cacgcgtcgt taagagggca
     1021 ggcaacttga attgttgcct gccctcttct tataacatcc ctgcccttct cagacacctc
     1081 tgcgaataat cacaccagcc ccttctcctg ggcgtaccgc agcaatgcca tccggccgtc
     1141 cacgttcagc ttctcgtaga tgttgtgcag gtggaccttc accgtcccct cgctgatgca
     1201 gagcttgtcg gcgacgtcct tgttgcgaag gccgcgggcc accatgcgga ccagttcgat
     1261 ctcccggggg gtaaggatgg cggccgcctc gcgctcccct gtttcccgac gcagcatctt
     1321 ctccagtacc tgtttggcgg agcggcgctc tatccactgc tcgccggcgt gcactttgcg
     1381 gatgcactgc acgagaaact gcggcgccat ctccttcaga acgatgccgt tcacctccag
     1441 atggaccgca tcgagcatct cctcgtcttt gagcgccgca gtgagaagca ccactcgcgt
     1501 cggcagtttt tcctcgcgaa ttttccgggc gacctccagg ccgctcattc cgggcatgct
     1561 gatgtccagg accgccacat ccggccggaa ctggcgcacc gcctgcatcg ccccgactcc
     1621 gtctgcgcaa ctggccagca catcgaagcc ttcctcgaga cgaaaaaggt tctccagccc
     1681 gttcaggata atgggatggt cgtcggccag caccaggcga attgacattt tatccccccc
     1741 tctcggtgag cggcaggatg atctccagac gcgcaccgtc ttcccgtgat tcgattgcca
     1801 gggtccccct cagggcggca atccgctcct tcaatgtcac ggggccccgc ttcatagcga
     1861 ataatgcgtc ttgatcataa ttgccgcgga aagagaaacc gtgcccgtca tcggcgaccg
     1921 tgatatgcac ccggtccgct ttgaaggcaa ggcccgcgcg cagatgagag gcgccggcat
     1981 gccgcacggc attgatcagc gcctcgtgga cgacaaaata gacctccctt gccatgctca
     2041 tagcgatccg gggtgcggca ggagcggtcg tgatctcgac cgaaaggtcc cactgccgcc
     2101 tgatccgttc ggccaattcc tccaggcggg aggtcagctc gaaatctcgt gccgggcggc
     2161 cctgaggaaa aggcctcagt tcgttgatat gggacctcag gtccctttgc tcggaagcga
     2221 gcaatcgctg gatttcccgg atgcgctgcc cggccgtctg aggctccgtt tccataaggc
     2281 gatgcgccgt ttcgagctgc agggccgcgc cggtcaacga ctgaaggagg ccgtcgtgca
     2341 ggtcgcgggc cagccggatc cgttcattcg acgccgctgc ttcttgctgc tgctttacga
     2401 ggtaacaacg gtcgaggcgg ccggcaacct catttgcgac gataccgccc agcaccaggt
     2461 cgtcggcggt catcggagtc ttgtcgagcg acaggagata ccctgctgct ttttccccgg
     2521 tgaaccttga ggcgaggatg gccctgacgg caaagttctt ctgcaattcc ggatcgagag
     2581 gagtcccctg ccatctccgc aagccggcac gatcactgtg tatgacggga gattgaggat
     2641 cgccggcatt ctggcagaaa aagcttgtgc cggacagttg ttcggcaacg agagttccaa
     2701 aaacggcggg aggttcacgt ctgtattgac agccatcccg cgaccacgag gccagatgga
     2761 gccatggttc ctccgcttcc tcccaggcca gaacaacgcg gggggcattg aggattgtcg
     2821 cagcatgccc gagtatctcg caactcagtg actgaggatc gtccgaaacg gaacggggcc
     2881 atcccgcaag cttggaaaac acgctgctca tcgactgttc gtattctccc agataaccga
     2941 gtaataccgc cacgactgcc aggtagacga cgcggatgat gaaacggttc agttcgaaat
     3001 tctggtcgtg cagcagattc gaaggaaacc atgcgagaaa aatgactacg gccagggctg
     3061 taaccgcggt ccataacgtc ccacgcctct gccagcggag cgtggcgcac acaagcagga
     3121 agataaaata gacaaaaaac ggactggtcg acccttcggt gagaaacatg aggagtgcga
     3181 agaccagcat atctatggca tgggtcacga catgcagacg gcccttgttg aagtactgtt
     3241 tcaacgtcat aacgcccaaa aggagggagt agatgaggta gccggccaat attgcgtagg
     3301 taagccgggc ataccgggaa ggctccgaag ggtcgatcca gattgccaga agaaaaaagg
     3361 ccgaaagaat catccgcccc gtggcgatga gccgttccgc ccgcctctgc ggaacatact
     3421 gctttcccgc gtgcagggac gccgtcgaat gttccatgaa tacaccgcct ttggagaccg
     3481 agtccaaaag ttcctcccgt gatgcagcgg catgggaaat aatggatata tgccggagcg
     3541 ttttcccctg tcaaaaaaag aaatgacctt gctcggctcc gatcacac
//
LOCUS       DASSLD010000331         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1438881, whole genome shotgun sequence.
ACCESSION   DASSLD010000331 DASSLD010000000
VERSION     DASSLD010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438881"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VFK23_06000"
     CDS             complement(<1..521)
                     /locus_tag="VFK23_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318671.1"
                     /translation="MTCKARTSLLLAVICFLIFPRSSSAEIDVTSKTYVRFFRDARDL
                     QYAPLNEYVELDGRDQQQGKWDFHVSAWGAYDFKTEQFGDKTRDELTYAYLRYSPYQD
                     RRLLLYAGRHYVFEGVATEQIDGVSARWETTSSNGISLFGGSPVVTAFDSRRGDAAYG
                     GRLYQRFSSRAELG"
     gene            complement(561..968)
                     /locus_tag="VFK23_06005"
     CDS             complement(561..968)
                     /locus_tag="VFK23_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET7318672.1"
                     /translation="MQLTREGDYGIRSVLYLARQPFKKVSFVNEISEEYKIPRSFLAK
                     ILQKLVKAKIVRSYRGVKGGFSLARQARDITVLEVIEAIEGRLALNICITDKKKCSFS
                     KQCPSHILWYNVQSKVTDMLKKANFEDLARQKP"
     gene            1093..1749
                     /locus_tag="VFK23_06010"
     CDS             1093..1749
                     /locus_tag="VFK23_06010"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET7318673.1"
                     /translation="MKSAGIITLCAAGIFLLSAFAPAHQAGAAELSGVAHPAGATLRL
                     VSENGQKATVTTAPDGRYEFGTLSDGKYILQASAPGYASDSLEVRISEGRSAKLNLRL
                     LYISRIDGIDWSKGIIRATGRGLYPADSPNRPAAREMAKRAALSDAERNLLGAVEQIK
                     VGPNQDLISSAARSYIVRIKGYLQGFRVVGERETDSGIEVELELPLTGPDGLTRIISD
                     "
     gene            1773..2327
                     /locus_tag="VFK23_06015"
     CDS             1773..2327
                     /locus_tag="VFK23_06015"
                     /inference="COORDINATES: protein motif:HMM:NF025743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HET7318674.1"
                     /translation="MGQIRPPLPVKLFVGMLSPEPELFDACSQILQRDFGPIAGVSAC
                     MPWDSSDYYRGEMGPNIFRKFLFFEQIVDPGRLSQIKNFTNALEDNYAANAADGLRRR
                     INLDPGYVTEAKVVLASTKDFAHRVYIGDGIYAEVTLKYSAKDRSFVPHELTYPDYRT
                     APQLALFNSVREDLRTRLRATRPT"
BASE COUNT          528 a          703 c          671 g          548 t
ORIGIN      
        1 ccgagttccg cgcgagagga gaatctctgg tacagcctgc cgccataggc ggcatcgccc
       61 cttcgggaat cgaatgccgt cacaaccggc gacccgccga acagggaaat gccattggat
      121 gacgtcgtct cccagcgcgc cgagaccccg tcgatctgct ccgtggcgac accctcgaag
      181 acgtagtgcc ttcccgcgta caaaagcagc cgtctgtcct ggtagggtga atagcggaga
      241 taggcatacg tgagttcgtc gcgggttttg tccccgaact gctcggtctt gaagtcgtat
      301 gcaccccacg cggaaacgtg gaagtcccat ttaccctgct gctggtccct tccgtccagt
      361 tcgacatatt cattgagggg tgcgtactgc agatcccgcg cgtctcgaaa gaatctgaca
      421 taggtcttcg acgtcacgtc gatttcagcg gaagagcttc ggggaaagat caggaagcat
      481 atgacggcaa gaagcagtga tgtccgggct ttgcaggtca tgtactggct ctttcgtgga
      541 cgtaagaata tctggaatga tcagggcttc tgcctcgcca ggtcttcaaa attcgctttt
      601 ttcagcatgt ccgtaacttt tgattggacg ttgtaccaca gaatatgaga ggggcactgt
      661 ttggaaaaac tgcatttttt cttgtcggtg atgcagatat tgagcgcgag tctgccttcg
      721 atggcctcaa tcacttcgag gacggtaata tcacgggcct gccgggcaag tgaaaaaccg
      781 cccttgacgc cgcggtacga ccgaacaatc tttgccttca ccagcttctg gaggatcttg
      841 gccagaaaac tcctcgggat tttgtattcc tccgatattt cgttgacgaa actaactttt
      901 ttaaaaggct ggcgggcaag atacaagacg ctgcgtatgc cgtaatcacc ttcgcgggtc
      961 agttgcatgt ttttagtata tcgaaccgta tgtcaatgtc aaggttaaac cgggagcttt
     1021 ttgaacgtca gccggactca atctttcagg gagaaaatga tttgtgatat aatgagccca
     1081 tcaggaggcc gcatgaaatc tgcgggtatc attactcttt gtgcggcagg cattttcctg
     1141 ctctctgcct tcgcgcccgc gcaccaggcc ggcgcggcgg aactctcggg agtcgctcat
     1201 ccggccggtg caacgctgcg tttggtctct gaaaacgggc agaaggcaac cgtgaccacc
     1261 gctcccgacg gaagatatga atttggaact ctttccgacg ggaaatatat tcttcaagct
     1321 tccgctcctg gatatgcaag tgactcgctc gaggtccgga tctccgaggg acgaagcgcc
     1381 aagctgaact tgaggctcct ttacatttcc cggattgacg gaatcgactg gtcaaaggga
     1441 atcatccgcg caacaggcag aggcttgtat cccgcggact cgccaaaccg gcctgccgcg
     1501 cgtgaaatgg caaaacgcgc cgcactttcc gacgccgaac gcaatctcct cggggccgtc
     1561 gagcaaatca aggtcgggcc gaaccaggac cttatttcct cggccgcccg cagctacatc
     1621 gtcaggatca agggctacct tcagggcttc cgcgtcgtcg gcgagcggga aacggattcc
     1681 ggcatcgagg tggagcttga gctccccctg accggaccgg acggcttgac gcggatcatt
     1741 tcagactgag gccgttttct accggggtca gcatgggtca aatacgtcca cctctgccgg
     1801 tcaagctgtt cgtcggcatg ctgtcgcctg aacccgaatt gttcgacgcc tgctctcaaa
     1861 ttctacaaag agacttcggc cctatcgctg gagtcagcgc atgcatgccc tgggacagct
     1921 cggactacta ccggggggaa atggggccga acatattccg gaagtttctc ttcttcgaac
     1981 agattgttga ccccggcagg ttatcgcaga taaaaaactt caccaacgct cttgaggata
     2041 actacgccgc gaatgccgcc gacggactcc ggcgaaggat caacctcgat cccggctatg
     2101 ttaccgaggc caaagtcgta ctcgcatcca ccaaggactt tgcgcaccgt gtctacatcg
     2161 gcgacggcat ctatgccgag gtcaccctga agtacagcgc gaaagaccgg agtttcgtcc
     2221 cccacgagct tacctatccc gattaccgca cagcgcccca gctggcgctg ttcaacagcg
     2281 tccgggagga tcttcggacg cgcctgcgcg caacccgccc cacttgagcc gtcggcaccg
     2341 ggtgcctcga cattattcag aaggattgaa gatgatcttc acggcatctt gcgtcagcgg
     2401 tgcatcgcgg cgcgcgtgtt cgcttctttt ctgaatccgt agcgtcctca
//
LOCUS       DASSLD010000332         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1210381, whole genome shotgun sequence.
ACCESSION   DASSLD010000332 DASSLD010000000
VERSION     DASSLD010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210381"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2115)
                     /locus_tag="VFK23_06020"
     CDS             complement(<1..2115)
                     /locus_tag="VFK23_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HET7318675.1"
                     /translation="MMKRIVHQLMAVTLSAVLLSCAGSQAFQRGEKYAQRGEWDLAVK
                     EYREANKRNPQDIEYRSALLRAQETAANEHYKRARSFLKERKFDPAIVELQQAIYLNP
                     TNVAIQSALKSVLGMKEAEDHYRASLTFVELNRLGEAINELTLAINLDPENVKYQDAL
                     DKLQKKKTEIEPEEALSLVSEKPISLNFKNTNIKDVFEFLAKLSGINILFDEEVKAQP
                     VTVFVKDVSFQYALNLLLSTNKLFMKKISADTIIIIPKNKAKTDQYQDLIVRTFYLNF
                     AKSKEIVNLVRSMLDVKRVYVNETLNSITIRETPEKMKLVEKIIAANDLKEAEVILDV
                     EILEISRTNALKYGWNFQPGLSTTATIQPTVTTTTSAVSANAGIALSELRSLNSDNVF
                     LSLPSLVFNLIKQDSDAQTLANPRVRILNNKQAKFHIGDKIPVQTSTVQATTAVAVTS
                     TFEYKDVGIKVSIEPTVHLSNTVTLKLGLEISTLGDALEFGNGQKQFRFGTRNTDTSI
                     NLRDGETVIIGGLIQDQDRKTRNKIPLLGDIPILGKLFSSEDDGTIKTDILMSITPNI
                     VRNMELPDKETQAFWSGTESDFDTKPLFVSAGKSTKPSEKPLDKTAVLESMAKREAAT
                     PAGAAVSAPTPTAAAQPPVEGAPALLEMKPADTAALVGQEMRYEITAGSVKDLYGVIL
                     TLSYDPKIVEFKTAGEGTLLKKD"
     gene            complement(2275..>2503)
                     /locus_tag="VFK23_06025"
     CDS             complement(2275..>2503)
                     /locus_tag="VFK23_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318676.1"
                     /translation="GYLTSKDNMLFLSKDGETFIVRSGDKLLASYQVKEAGKDYVILL
                     DTATRVEVRVELSGSEGAQPPQPQPGMPRFR"
BASE COUNT          410 a          728 c          743 g          622 t
ORIGIN      
        1 gtccttcttg aggagcgtcc cttctccggc ggtcttgaat tccacgatct ttggatcgta
       61 actgagcgtc aggatcacac cgtaaaggtc tttgacgctt cccgcggtga tctcgtaccg
      121 catctcctgt cccaccaagg ccgctgtgtc cgcgggcttc atctccagaa gggcgggcgc
      181 cccttctacg ggcggctgcg cggcggcagt cggcgtcggc gccgacaccg ctgcacctgc
      241 gggggtcgcc gcctcgcgct tggccatcga ttcgagaacg gccgtcttgt cgagcggttt
      301 ttcagaaggt ttcgtgctct ttccggcgga gacaaagagc ggtttcgtgt caaaatctga
      361 ctcggtgccc gaccagaagg cctgcgtctc cttgtccggc agttccatgt tccggactat
      421 gttcggcgtg atcgacatga ggatgtcggt cttgatcgta ccgtcgtcct ccgaggagaa
      481 gagcttgccg aggatcggaa tgtctccgag aagcggtatc ttgttccgcg tcttgcggtc
      541 ctggtcctgg atcaggccgc cgatgatcac cgtctccccg tcgcggaggt tgatcgaggt
      601 gtccgtgttt ctcgtgccga acctgaactg cttctggccg ttgccgaatt ccagcgcatc
      661 gcccagggtg ctgatttcca gtcccagctt gagcgtcacc gtgttgctga ggtgaaccgt
      721 cggctcgatg gagaccttga taccgacgtc cttgtattcg aaggtggacg tgacggctac
      781 cgccgtggtg gcctggacag tcgaggtctg gaccgggatc ttgtcgccga tatggaactt
      841 ggcctgcttg ttgttgagga tccgcacccg gggattcgcc agcgtctggg catccgaatc
      901 ctgcttgatc aggttgaaca cgaggctcgg aagcgacagg aacacgttgt cgctgttcag
      961 gctcctgagt tcgctcaggg caatgccggc attcgcgctc acggcggagg tggttgtggt
     1021 caccgtcggc tggatcgtgg cggtcgtgga aaggcccggc tggaaattcc agccgtattt
     1081 cagagcgttc gtccgcgaga tctcgaggat ctccacgtcg aggatgacct cagcctcttt
     1141 cagatcgttc gccgcgatga tcttctcgac cagcttcatc ttttccggtg tctcacggat
     1201 ggtgatggag ttcagcgttt cgttcacgta cacccgcttg acgtcgagca tggaacggac
     1261 caggttgacg atctccttcg acttcgcgaa gttgagataa aaggtcctca cgatcaggtc
     1321 ctggtactgg tctgtcttgg ccttgttctt cggaatgatg atgatcgtgt cggcgctgat
     1381 cttcttcatg aagagcttgt tcgtggagag gagcaggttc aacgcgtact ggaacgacac
     1441 gtccttgacg aagacggtga cgggctgggc cttgacttcc tcgtcgaata gaatgttgat
     1501 gcccgaaagc ttggcgagga actcgaaaac gtccttgatg ttcgtgttct tgaagttgag
     1561 cgagatgggc ttttccgaga ccagcgagag ggcctcttcg ggctcgatct cggttttctt
     1621 cttctggagc ttgtccaggg cgtcctggta cttgacgttc tccgggtcca gattgatggc
     1681 cagcgtcagc tcgttgatgg cctcgccgag ccgattgagt tccacgaagg tgagcgacgc
     1741 gcggtagtgg tcctcggcct ctttcatgcc gaggaccgat ttgagcgcgc tctggatcgc
     1801 cacattggtg gggttcaggt agatggcctg ctggagttca acaatcgcgg ggtcgaattt
     1861 gcgctccttc aggaagctgc gggccctttt gtagtgctcg ttcgccgccg tttcctgggc
     1921 ccggagaagc gcagaccggt actcgatgtc ttggggattg cgtttgttgg cctcacggta
     1981 ctccttcacc gcgaggtccc actcgccgcg ctgggcgtat ttctccccgc gctggaacgc
     2041 ctgtgagcca gcgcagctta agagcaccgc agacagggtg actgccatga gctggtgcac
     2101 gatcctcttc atcatcgata atcctccctg taatgaaacg aaagacgatg gacgatcacc
     2161 ctgagatgca tgacggacga tggacgaata atcttttcgt ccctcgtctc tcgtccttcg
     2221 tccctcagtt tctacccctc gtccttcgtc tctcatccct cgtccctcat cccttcacct
     2281 gaacctcggc attcccggtt gtggttgcgg aggctgcgcc ccttcactgc ccgacagttc
     2341 cacgcgaacc tcgacgcgcg tcgccgtgtc gagcaggatc acgtagtcct tgcccgcctc
     2401 tttcacctgg tagcttgcga gcagcttgtc cccgcttcgt acgataaagg tctcgccgtc
     2461 cttcgacaga aacagcatat tgtccttgct ggtcaggtac ccc
//
LOCUS       DASSLD010000333         4410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1436965, whole genome shotgun sequence.
ACCESSION   DASSLD010000333 DASSLD010000000
VERSION     DASSLD010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436965"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3637)
                     /locus_tag="VFK23_06030"
     CDS             complement(<1..3637)
                     /locus_tag="VFK23_06030"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7318677.1"
                     /translation="MIQYRFDVVIIGAGPAGVTAAGALAGSGLSVALLEAGVYAGAEN
                     WSGCVYFAESLAAQDCFGPAAVEAAPFERRVARRGTLLHNGLDVVGVELAEPSAFRNC
                     YTVLRPIYDPYFAHLAAAKGAVHITETTVTSLIRRDGRVIGVQTNRGPLYAEVVFIAE
                     GDASHLVRSERLERVPEPHYLQGVKAVFALPPEEIEKRFNLGAGEGCAYEFLVRNARV
                     AGRTAKLNVGGFLYTNRDSLSLGYVVPLDNLRDHYRGDHDRLFEWMRGLPFIRELAAN
                     AKLSAYGTKIVRGGGWSERPVLVEDGLAVGGASTGLGVDIPFPNFTGPASATGLLFAR
                     AAKAILGSGRVPDAKNLAKEYLAPLEESVYGKNAEYLSRWPQYFGKSSVLFGRFTDKA
                     CGIAHFLSSGGYVETGRFLNNHIISYRKAKESIFDILRGISSLRLWKPILKTVANPGT
                     WIKWAANVFSSKPSSDKGDCIILNMGGRPIKVSSLPWPAGSFLERISPAFSGALQSIY
                     SNDEVPAEKKFARSVRIIIRSVSLVDLFILPLFGFTLFVLALLTAAWDAVRFYLLRTP
                     VEKLLSEPVMAYNEAQRKARDLDAVKPAIGFEAKLATNTYRVGNTSHIRTLWPEAIAS
                     QPEMSRASLWWICPARVYGYDAPLAGRGKVTVNFENCIKCESCWRAEPRVALWGRHTN
                     HGLIYRPESSAIPDLLRSLESNHPSPVAKGGLGISDIDKESGNLNREVVQASREAKTA
                     SAAFEDAVSKLPASADPGRKAWPLALGRRLGAKLRKLESALAADGRHELVQLVLSDRN
                     DIERRLDEYGLFHALYCCRRLNERLDAWLSSSSARPEPAFAAPAPAAGGARQPDQGLL
                     PLTYQEVSSIFPDRIVKEWEEAPLPDTWAGKLREFIAARRDSPLETVRVLSSVSPALG
                     LVAAHQFAALSALKKAGITLDPGICAVRADDLEIRESIDSVHLKGTIALIPLAACSSL
                     LVLVGERGHLVPLSSAGVTITPAPAIGFRAAGLADIVLDCSVKKYAIVAPDRPEVPGA
                     ASYLAVALGAGDYLCRRTKEHAVGRVQFPGQMLDTEGRDGIAKLGAVKAMIARTESWR
                     LLLETLFDFYSEFRISVARFTGHPDSAFEFDSLCSTLAALAFSPEPGSMGYDAGQVFG
                     GFAYSEDDLLSRSYRDSALFRFLSPGYGTADKLNAILKSDALNSASAWPVSLDILLPN
                     SAFSDEPI"
BASE COUNT          896 a         1272 c         1363 g          879 t
ORIGIN      
        1 cgatgggctc atcgctaaat gcggaattcg gaaggaggat gtccaatgaa acgggccacg
       61 ctgatgcgga attcagagcg tcactcttga gtattgcgtt taatttatcc gccgtcccgt
      121 aaccgggaga gaggaaccgg aagagcgcgc tgtctcggta ggagcgcgac aggaggtcat
      181 cctcggaata ggcaaaccct ccgaagactt gtccggcgtc gtagcccatg ctgccgggct
      241 cggggctgaa ggccagggcg gcgagggtgg agcagagaga atcgaattca aatgcggaat
      301 ccggatggcc ggtgaagcgg gccacgctga tgcggaattc ggaatagaaa tcgaacagcg
      361 tttcaaggag aaggcgccag gactcggtcc tggcgatcat ggcctttacc gcgccgagct
      421 tcgcgatgcc gtcgcgtcct tcggtatcga gcatctggcc ggggaactgg actcgtccga
      481 cggcgtgttc cttggtcctc ctgcagagat aatcgcccgc ccccagggca acggccaggt
      541 aggacgccgc gccggggacc tcgggccggt ccggcgcgac gatggcgtat ttcttcacac
      601 tgcagtccag gacgatatcg gcaagtcccg ccgcccggaa gccgatcgcg ggggccggcg
      661 tgatcgtgac accggccgat gacagcggga ccagatgccc cctttccccg acgagaacaa
      721 ggagggaact gcatgcggcc aggggaatca gagcaatagt tcccttgagg tggacgctgt
      781 cgatgctttc ccggatctcg aggtcgtcgg cgcggacggc gcagatgccg gggtcgagcg
      841 tgatgccggc ttttttcaat gcggacaatg ccgcaaactg gtgcgccgcg acgagaccga
      901 gcgccgggct gaccgaagag agcacgcgga ccgtctcgag cggactgtcc cgtcgcgcgg
      961 cgataaactc gcggagcttt cctgcccagg tgtcgggcag gggcgcctct tcccactctt
     1021 tcacgatcct gtccgggaag atcgacgaca cttcttgata agtgagcggc agcagcccct
     1081 ggtccggctg tctggctccc cccgcggcgg gggccggagc ggcaaaagca ggctccgggc
     1141 gggcggagga gcttgacagc cacgcatcga gacgctcatt gagccgtcgg cagcagtaaa
     1201 gtgcgtggaa aagcccgtat tcatccagcc ggcgctcgat gtcgttccga tcggacagca
     1261 cgagctgcac cagttcatgc ctaccgtcag ccgcaagggc tgattccagt ttccggagct
     1321 tcgcgccgag ccgtctcccg agcgcaaggg gccaggcctt tctgcccggg tctgcggacg
     1381 cgggcaactt cgagacggca tcctcgaaag cggccgatgc cgtctttgct tcccgggagg
     1441 cctgaacgac ttcacggttc aggttccctg attctttgtc gatgtcggaa atccccagcc
     1501 ccccttttgc gacgggggat ggatggtttg attcaaggct ccgcagcaag tccgggatcg
     1561 cgctgctttc gggccggtag atgagcccgt ggttcgtgtg cctaccccag agggcgacgc
     1621 ggggctcggc cctccagcag gattcgcact tgatgcagtt ttcgaaattc acggttacct
     1681 tgccgcggcc cgcgagaggc gcatcgtagc cgtacacccg cgcggggcag atccaccaga
     1741 gcgatgctcg cgacatctca ggctgagagg cgatcgcttc gggccagagt gttctgatgt
     1801 gcgacgtgtt ccccacacgg taggtgttcg tcgcgagttt ggcctcaaac ccgatcgcgg
     1861 gtttcacggc atcgaggtcc cgcgcctttc tctgcgcctc attgtatgcc atcaccggct
     1921 cggagaggag tttttcgacc ggcgtcctga gcaggtagaa tcggacggcg tcccaggcgg
     1981 cggtgaggag agccaggacg aagagggtaa aaccgaagag cggcagaata aaaagatcca
     2041 caaggctcac ggaccggatg atgatcctca cggaccgggc aaattttttt tccgccggca
     2101 cctcgtcatt ggagtagatc gactgaaggg cgccggagaa cgcgggggaa atacgctcga
     2161 ggaacgagcc cgcgggccag ggaagggagg agaccttgat gggcctgcca cccatgttca
     2221 ggatgatgca atcgccctta tctgacgacg gtttcgagga gaagacattg gcggcccact
     2281 tgatccatgt gccgggattg gccacggtct tcaagatcgg cttccagagc ctgaggctgc
     2341 tgatgccacg caggatgtcg aaaatcgatt cctttgcctt gcggtacgag ataatgtgat
     2401 tgttcaggaa cctcccggtc tccacatagc ccccgctcga gaggaaatgc gcgatgccgc
     2461 aggccttgtc agtgaacctt ccgaacagca cggacgattt cccgaaatat tggggccagc
     2521 gggagagata ctctgcgttc tttccgtaca cgctctcttc gagcggcgcg agatattcct
     2581 tcgcaaggtt ctttgcatcg ggaacgcggc cgctgccgag gatggccttg gccgcccgcg
     2641 cgaacaggag ccccgtggcc gacgccgggc cggtgaagtt cgggaaggga atgtcgacgc
     2701 cgagccccgt ggacgcccct cccacggcga gaccgtcctc gacgaggacc ggtcgctcgc
     2761 tccatccgcc gccccggacg atcttggtgc catatgccga gagcttcgcg ttagcggcga
     2821 gttccctgat gaaaggcagg ccccgcatcc attcgaagag ccggtcgtgg tcgccccggt
     2881 agtggtcccg caggttgtcc aggggaacga cataaccgag ggaaagactg tccctgttcg
     2941 tgtacaggaa gccccccacg ttcaatttcg cagttctgcc cgcgacccgt gcgttcctga
     3001 cgaggaactc gtaggcgcag ccttcgcccg cgccgaggtt gaaccttttt tcgatttcct
     3061 cgggcgggag cgcgaagact gccttgacgc cctgcaggta gtgaggctcc ggaacgcgtt
     3121 cgagccgttc cgagcggacg aggtgggagg cgtcgccctc ggcgatgaat acgacctccg
     3181 cgtagagagg cccacggttc gtctggacgc cgatgaccct cccatccctg cggatgagcg
     3241 aggtgaccgt cgtttccgtg atgtgcaccg cgcctttcgc ggctgccagg tgcgcaaagt
     3301 aggggtcgta gattgggcga agcacggtgt agcaattcct gaaggccgaa ggctcggcga
     3361 gctcgacgcc caccacgtcc aggccgttat ggagcagcgt gcctcgccgc gccacgcggc
     3421 gttcgaaagg ggcggcctcg accgcggcag ggccgaagca gtcctgcgcg gcgaggctct
     3481 cggcgaagta gacacagccg gaccagtttt ccgctcccgc atataccccg gcctcgagca
     3541 aggcgacgga taatccgctg cccgcgaggg cccccgcggc agtcaccccg gcggggcccg
     3601 cgccgatgat gacgacgtcg aaccggtact gtatcatgga tgctcctgtt atcactcttc
     3661 gccgtaattc cccaatgctt tcatcctcga tagattcact cgaggacgga tccagatttt
     3721 tggattgtaa ataagcacca gatccacgat agaatcatcc ggaatgatga caccttttgg
     3781 gctggaaatg ggaatggata tctaaacaat aagcaaatta tagcagaaca atcagaaggt
     3841 tgagcgggcc tgacaggttt agatttaata atttgtatct cccgggaatg tcatttccgc
     3901 gaaatcagga gcccatgcga tagatcttag ctaagagccg cgtccccgcg cacgcgtgcc
     3961 ttaatccaag gggcttgcgg ctttctgcaa tgagggcgaa agagagaggc ttcctgcaca
     4021 agcgaatgac tcggatcgct tggagcaagg aagaaggccg aaggttcgat gatgattggc
     4081 ttccactcga cacggatcct gatcccgcag catagagtct gtgcagagat tacaatatat
     4141 tgaacttaat ttcggttact tttcgtatct tttttttctt catcacaatt actttttgtt
     4201 acgaagttcg agaaaaaatg gcgtttccga acttgtcgaa taccaggtca gaaaaaaaat
     4261 ctctggctgc gcattttgta atgtgcaatc ttctataaac ctgaatttta tatcatattt
     4321 gaattcatgc tttgtctctc tttgataaaa ctgttttttt cgtcgaaaac acgttgaata
     4381 ttatcagtaa ctaactcatt gcggcccacc
//
LOCUS       DASSLD010000334         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_69752,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000334 DASSLD010000000
VERSION     DASSLD010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69752"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..475
                     /gene="rplN"
                     /locus_tag="VFK23_06035"
     CDS             107..475
                     /gene="rplN"
                     /locus_tag="VFK23_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET7318678.1"
                     /translation="MIQQQTVLDVADNSGAKKVMCIKVLGGTRKRYATLGDVIVVAIK
                     MAIPEGQVKKGTVAKAVVVRTTKEVRRQDGSYIKFDRNAAVLINNANEPIGTRIFGPV
                     ARELRKKNFMKIISLAPEVL"
     gene            521..832
                     /gene="rplX"
                     /locus_tag="VFK23_06040"
     CDS             521..832
                     /gene="rplX"
                     /locus_tag="VFK23_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET7318679.1"
                     /translation="MQIKKNDNVMVTSGKEKGKRGRVIAVYPKTNRILVEKLNMIKRH
                     TRPNQQLRQGGIVEKESPISVSNVRLICSKCDKPTAVSRQDQGGGSRARVCRTCNEAI
                     D"
     gene            1063..1596
                     /gene="rplE"
                     /locus_tag="VFK23_06045"
                     /pseudo
     CDS             1063..1596
                     /gene="rplE"
                     /locus_tag="VFK23_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
     gene            1626..1811
                     /locus_tag="VFK23_06050"
     CDS             1626..1811
                     /locus_tag="VFK23_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET7318680.1"
                     /translation="MAKKSLIAKAQRSSKFKVRNYHRCPLCGRSRGYLRKFDMCRICF
                     RNRALRGEIPGVIKASW"
     gene            1831..2226
                     /gene="rpsH"
                     /locus_tag="VFK23_06055"
     CDS             1831..2226
                     /gene="rpsH"
                     /locus_tag="VFK23_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET7318681.1"
                     /translation="MMTDPIADMLTRIRNASKAKHEKVDIPSSKLKIEIAKILKDEGF
                     VKNVKLVKDRRQGLIRVYLKYTDEELPVIQGLKRVSRPGCRVYAGNDAIPKVMAGLGT
                     AILSTPKGIQTGKQAKKDNVGGEVLCHVW"
BASE COUNT          641 a          529 c          689 g          403 t
ORIGIN      
        1 atagagaccc ggcccctgtc caaggaaaaa cggtggaagg tgctggagat cattgagaag
       61 gccatttaaa cgcaggtgaa gaaaaatctc cgttgaggat tgagacatga ttcagcaaca
      121 gacggtattg gacgttgccg ataattccgg cgcaaaaaaa gtaatgtgca tcaaggtgct
      181 gggcggcacg cgcaagcgct acgccacgct tggtgacgtg attgtcgttg ccatcaagat
      241 ggcgatccct gaaggtcagg taaaaaaggg aacagtggcc aaggccgtgg tcgtccgcac
      301 gacgaaggaa gtgcggcggc aggatggttc ttacatcaag ttcgaccgta atgcggcggt
      361 tttgattaac aatgcgaacg agccgatcgg gacgcgcatc ttcggtcccg ttgcacgtga
      421 gctccgcaag aaaaacttta tgaagatcat ttcactggca cctgaagtgc tctaaagaag
      481 ggcgccgatg ccggcttgag gaaaagcaga aagtgcgact atgcagataa agaaaaatga
      541 caacgtaatg gtaacctccg gcaaggagaa ggggaagcgg ggcagggtca ttgccgttta
      601 tccaaagaca aacaggattt tagtggaaaa actgaacatg atcaagcggc acacgcgacc
      661 caaccagcag ctcaggcagg gcggcatcgt agaaaaggaa agcccgattt cagtctcgaa
      721 cgtacggctg atctgctcca agtgcgacaa gccgacagcg gtttcgcggc aggatcaagg
      781 cgggggttcc cgcgcgaggg tgtgcaggac ctgcaacgag gctattgatt aaatgactgc
      841 cccgtacgga ctataccgta aaagcttata aaacaggaca atcgatatat ggcggataaa
      901 aaagacaaat cgaaagaagc gaaaagcggc gagcagaaaa aagctgccgc aaagtccccc
      961 aaggcagcgg caaaagcaaa ggcccccgca gcggctggaa aaaaggaagc ggggcaggcc
     1021 gagggcggca aaaaaagcgc acccgagcgc gtcccgacgc cacggctcaa gcagcggtat
     1081 cacgacgtcg cggtccccgc gcttatgaag gaattccagt acaagaaccc catgcaggcg
     1141 ccaaagcttg tcaaggtggt cctgaacatg gggatgggcg aggcaataac caatgcaaaa
     1201 gccctcgacc acgccgccga ggaaatggca aagatttcgg ggcagcgccc cgtcatcacc
     1261 cgtgccaaga agtccattgc cacgtataag ctgagagaag ggatgccgat cggatgcatg
     1321 gtgacgttga ggcgcgagcg catgttcgag ttcctggacc ggttcatcag cgcggcgctt
     1381 ccccgcatca gggatttcaa gggtatttca tcgaaatcct ttgacggcag aggaaattat
     1441 tcactgggga tcaaggagca ggtgatattc cccgagatcg agatcgacaa ggtggactcg
     1501 atccacggta tggatattgt gattgttacg acggcgaaga ccgacgaaga gggcagggca
     1561 ttgctcagcc acctgggtat gccgtaccgg aagtagtccg gccagccgga cgtaatggag
     1621 gaacgatggc aaagaagtcg ctcatagcaa aggcgcagag atcctcgaag ttcaaggtgc
     1681 ggaattatca ccgttgcccg ttgtgcgggc gatcacgggg gtatttgaga aaatttgaca
     1741 tgtgcaggat ctgtttcagg aaccgggcgc ttcggggcga aatcccgggc gtgataaagg
     1801 cgagctggta aacaaaaggg gtttgaacca atgatgaccg atccgatagc ggacatgctg
     1861 acgcgtatcc gcaacgccag taaggcaaag catgaaaaag tagatatccc ttcgtcaaag
     1921 ctgaagatcg agattgcgaa gatcctcaag gatgagggat ttgtgaagaa tgtgaagctg
     1981 gtaaaggacc gcaggcaggg cctgatccgg gtctatctta agtataccga cgaggagctt
     2041 ccggtcattc aaggactgaa gcgggtgagc aggcccggct gccgtgttta tgccggcaat
     2101 gacgccattc ccaaagtcat ggccggtctt ggtaccgcaa tcctgtccac gccgaaagga
     2161 atacagacag ggaagcaggc gaaaaaagat aacgttggtg gagaagtcct ctgccacgtg
     2221 tggtgagttt caaacagtga tttcgtcctc taagagagtg ta
//
LOCUS       DASSLD010000335         3004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1001569, whole genome shotgun sequence.
ACCESSION   DASSLD010000335 DASSLD010000000
VERSION     DASSLD010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001569"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1099
                     /locus_tag="VFK23_06060"
     CDS             23..1099
                     /locus_tag="VFK23_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7318682.1"
                     /translation="MLRIPFFASLVLVLSVGPVLAASYPEPSSGDFIMRDFRFKSGEI
                     LPELRIHYRTIGTPKRDASGRALNAVLILHGTGGSGESFLTDYFAGELFGPGQLLDAT
                     RYFIILPDGIGHGNSSKPSDGLHARFPHYTYDDMVEAQERLLTEKLGVDHLRLILGTS
                     MGGMHAWLWGERHPDFMDALMPLASLPVEIAGRNRMQRRMIIDSIRSDPGWDNGEYET
                     QPRGLRSAVYIEIIMVSSPRRWQKEAPSREAADRLLDQMVSLRLARTDANDLLYQFDA
                     SREYNPAPDLGKIKAPLLAINSADDEINPPELGIMEKEIKRVRRGRYVLLPVSDDTRG
                     HGTHSYPAIWKQHLAELLRETEKR"
     gene            complement(1194..2072)
                     /locus_tag="VFK23_06065"
     CDS             complement(1194..2072)
                     /locus_tag="VFK23_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HET7318683.1"
                     /translation="MDKSKDKILVTGATGRQGGAVARYLLKSGYKVVVMTRKPQDEKA
                     GMLRSLGAEVIQGDYDAPKSCDPALEGIWGVFAVQNTWEAGVEREEEQGKRFAELARK
                     KGVIHYVYASVGSAHRNTGIPHFDNKWRIEETVRGLKFPSYTILRPAFFMENFTSPSF
                     LPGLLEGKLLMGLKAETKLQMIAADDIGRFGFLAFDQHEKMNGVELDIAGDEHTMPET
                     AEILGRAMGRSIRFVEVPKEEVRKMSEDYAIMLEWFDRVGYNVDIAALAQKYGIKPIK
                     LSEWAARVNWLARKAA"
     gene            2448..2750
                     /locus_tag="VFK23_06070"
     CDS             2448..2750
                     /locus_tag="VFK23_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318684.1"
                     /translation="MATVKNTKLYGYCDDVNKELASMKARLHELQDEVKTVYGADNEL
                     ALAHGRHLCELADMIEWKLQILMKACPFGWKGADKDIESVSVGSTEAEFSGGYIGG"
BASE COUNT          654 a          876 c          813 g          661 t
ORIGIN      
        1 taacgccggc agggagtaga aaatgctcag gataccgttt tttgcaagcc tcgtccttgt
       61 tttatccgta gggcccgtct tggccgcgtc ctatcctgaa ccttcctccg gcgatttcat
      121 catgcgggac ttccggttca agagcggaga gatcctgccg gagctcagga tccattaccg
      181 gaccatcgga acgccgaaac gggacgcgtc gggaagggcg ctcaatgccg tcttgatact
      241 gcacgggacc ggcggaagcg gggagtcctt tcttaccgac tacttcgcgg gcgagctctt
      301 cggaccggga cagcttctgg acgcgacccg ctacttcatc atcctgcccg acggaatcgg
      361 ccacggaaac tcgagtaagc cgagcgacgg gctgcatgcg cgctttcccc actataccta
      421 cgacgacatg gtggaggcgc aggagcgcct cctgacggaa aaactcgggg tggatcacct
      481 gcgtctgata ctgggaactt cgatgggggg aatgcacgcc tggctttggg gagagcggca
      541 tcccgatttc atggacgcgc tcatgcctct cgcctccctc ccggtcgaga tcgccggccg
      601 aaaccgcatg cagaggcgca tgataatcga ttccattcgc agcgatcccg gctgggacaa
      661 cggagagtat gagacccagc cgcggggcct tcggtccgcc gtctatatcg aaatcatcat
      721 ggtgagcagt ccccgccgat ggcaaaagga agctccgagc cgcgaggccg ccgaccgtct
      781 cctcgaccaa atggtgagcc tgcggcttgc caggaccgac gccaacgacc ttctctacca
      841 gttcgacgcg tcgcgggaat acaatcccgc accggacctg ggaaagatca aggccccgct
      901 tttggcgatt aactcggcgg acgacgagat caacccgccc gagctcggaa tcatggagaa
      961 ggagatcaag cgggtcaggc gcgggcgtta tgtgctcctg cccgtaagcg atgataccag
     1021 agggcacggc acgcattcct accccgcgat atggaagcaa cacctggcgg aattgctgag
     1081 agaaacggaa aagcgataaa acaattgatc ccgccccaag ccaggggaaa cgggttcaga
     1141 gttcacagct cacagattaa cttagaacta tgaactccga actatgaact tttttacgcc
     1201 gcctttctcg ccagccaatt gacgcgcgcg gcccattcag aaagctttat gggcttgatg
     1261 ccgtacttct gcgcaagcgc cgcaatatcg acgttgtagc cgacgcggtc aaaccattca
     1321 agcatgattg cgtaatcttc gctcatcttg cgcacttcct ctttcggcac ctccacgaac
     1381 cggatggacc tgcccatcgc gcggccgagt atctccgcag tttcaggcat cgtgtgctcg
     1441 tctccggcaa tgtcgagctc gaccccattc atcttctcat gctggtcgaa ggccagaaag
     1501 ccgaacctgc cgatgtcgtc cgcggcgatc atctggagct tcgtttcagc cttcaatccc
     1561 atcagcagct tcccttcgag aagtcccggc aggaatgaag gtgacgtaaa gttttccatg
     1621 aaaaaggccg gccggaggat cgtatagctc gggaatttga ggccgcgtac ggtctcttcg
     1681 atccgccact tgttgtcaaa atgaggaatg ccggtattgc ggtgcgcgct cccgaccgac
     1741 gcgtagacgt agtggattac tccctttttg cgggcaagtt ccgcgaacct tttcccctgt
     1801 tcctcttccc gctcaacacc cgcctcccag gtgttctgga cggcgaaaac cccccatatg
     1861 ccttccagcg ccggatcgca ggacttcggg gcgtcgtaat ctccctggat gacttcggcg
     1921 ccgagggacc tgagcattcc cgccttctca tcctgcggct tgcgtgtcat cactaccacc
     1981 ttgtagccgc tcttcaaaag gtaccgggcc acagctcctc cctgcctgcc cgttgcgccc
     2041 gtcacaagta ttttatcttt gcttttatcc atgatcagca cctccttaaa atctctcatg
     2101 atgccttttt ataattatat cattccgcgt atctacttaa gactttagtc ttcgacgttt
     2161 tttcggcttg ggggcctgcc ccgacttgtt cggggaaatc ggttattgac agaatggatt
     2221 tcagcttgga ttcccgccag gcttcaggca ctccggggaa ggcttagaaa caggaggcat
     2281 tgacgtcaaa ataagaagct ccggcgacag gaaacgccat gcataaataa taccatatta
     2341 ctcctctttt atttctcccg gccgcgcgaa tagcgttata cttaagtata gataacattt
     2401 gacacggagc gcattccagc gctctgtctt aagcaaagga ggaatatatg gcaaccgtaa
     2461 aaaatacgaa actctatggc tactgcgacg atgtaaataa ggagcttgcg agcatgaagg
     2521 cgaggctcca tgaacttcag gatgaggtca agacggtata cggcgcggac aacgagctgg
     2581 cgctcgctca tggacggcat ttgtgcgagc ttgccgatat gatcgagtgg aaactccaga
     2641 ttctgatgaa ggcctgtccc ttcggatgga aaggcgcgga caaggacatc gagagcgtct
     2701 cggtcggttc tactgaagcc gaattttccg gaggatacat cggcggttag cctgctcggg
     2761 aaagcaatta cgcaaaggcc ccgcagaaat ccgtctctaa aagcgacggg tgcggggcct
     2821 ttggcttttt ttcacaaatc gggagtttcg tcttcttcgg gatcgacctc gggcgggagc
     2881 tgctcgacgg tgtcttccgg ttcacagggc gcataaaatt gtcaagccga aagatggagc
     2941 gcgcttattc cctgccctct tcccgcatcg gcgcaaaccc cttccggatc gtgttctcgc
     3001 tgac
//
LOCUS       DASSLD010000336         3935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_687685,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000336 DASSLD010000000
VERSION     DASSLD010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687685"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..285
                     /locus_tag="VFK23_06075"
     tRNA            210..285
                     /locus_tag="VFK23_06075"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:243..245,aa:Cys,seq:gca)
     gene            449..>3935
                     /gene="dnaE"
                     /locus_tag="VFK23_06080"
     CDS             449..>3935
                     /gene="dnaE"
                     /locus_tag="VFK23_06080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET7318685.1"
                     /translation="MHHSDFVHLHLHTEYSLLDGAIGIDKLIKKAKDLKMPAVAVTDH
                     GNLFSALDFYLKASKSGIKPIIGCEIYVAPESRFDKTAPAGQNEEASYHLILLARNRQ
                     GYKNLVKLVTAAYLEGFYYKPRIDKDLLRAHSEGLIGLSACLGGEIPSLLIQGRYEDA
                     KRTALGYEQILGKENFYLELQDNGIPEQEQVNRELIRLNKDTGIPVVATNDCHYLDME
                     DHKAHDALLCIQTGKIVTDANRMRFSSETFYMKTPEEMKKSFSFIPEAVTNTVKIAER
                     CNLDLELGKYHLPHFPVPEGYTRESFMAELARKGLEERFVEIEAVRGPGSIDRAKYKD
                     RLEFEIQMIDKMGFAGYFLIVSDFIRYAKEHGIPVGPGRGSAAGSLVAYSLRITDLDP
                     IPYNLLFERFLNPERISMPDIDVDFCQDRRDEVIRYVTEKYGQDHVTQIITYGTMLAK
                     GVIRDVGRVLDIPYAEVDKVAKLVPNTLGITLEKAMEQEPRLKEIGKKDPRMAELMEI
                     ALALEGQVRHASKHAAGVVISQEPLTEYVPLFKTPKDEVTTQYDMKGVEKIGLVKFDF
                     LGLRNLTVIQKAEDLINQRLQKAPLAGTDPFSVSRVPLDDAKTFELLGRGETAGIFQL
                     ESSGMRDIVIKMKPGVFEDLIALVALYRPGPLGSGMVDDFIKRKKGSTKITYELPQLE
                     PILKDTYGVIVYQEQVMQISRTLAGYSLGQADLLRRAMGKKDPEVMAKEKVPFLKGAG
                     KQGIDLKKAEAIFDLMAKFAEYGFNKSHSAAYALITYQTAYLKAHYPVEYMAALLTSE
                     VADTDKVVKYIYEARQMNIDILPPDVNESRWDFTVIEAHDRESIEPGSTIGRIRFGLA
                     AVKNVGISAIEAMIEARETKGTFTSLCDFCKKVDQRRVNRRVIEALIKCGAFDFTGAR
                     RAQMMEAIDMMMEDAARHQEQEAIGQFSMFGCLDDKHEPALSNVPEWKESQLLAYEKE
                     SMGFYISGHPLAAFQDDMKRYATATTETLDSVPDGKEVTICGIISGLKQKITKKGDKM
                     AILNLEDLTGSVETIVFPDLYSTSQHLLLTDTPLIVAGQLDKSEQGNKIKAVRLHLLA
                     DVKKKGTTRIDISLSATGLTQDDLLKVKDILLRHRGDIPVYLRLPNPTRKDSLISVGR
                     DIRVTPSERLVAEIES"
BASE COUNT          924 a         1230 c         1044 g          737 t
ORIGIN      
        1 tatcgggacc catgttcatt tctctatcac ccccttcatc tcgaaacgtt ggtaggattg
       61 gcggcaatat actctctatg cgcgcagctt ccaaagcttc gttgataagc ttcctcttta
      121 atagttcaac ttgacaacaa tgcagttttc cattaatatc cagcttgtcc taaacagacg
      181 gaacgttaca cttcatcgat taaaagatcg gcggcgtagc caagtggcta aggcagaggt
      241 ctgcaaaacc ttcattcagc ggttcaaatc cgctcgccgc ctccatttta tagagtttac
      301 cgcggagata tagaggcgca gagaaaagcc aaaaattaat cattgtcttc atgatgatgc
      361 ctcccagttc cccgactctt tcttagatca ttcgcgacag tcgtcggcgg ttgacgttct
      421 ccgtgcctct gtggtgaaat aaattcctat gcatcactcc gatttcgtcc accttcacct
      481 tcacaccgag tacagcctcc tcgacggcgc gatcggcatc gacaagctca tcaagaaggc
      541 aaaggacctc aagatgccgg ccgtggcggt cacggaccac ggaaatcttt tctccgccct
      601 cgacttctat ctgaaggcct ccaagtccgg gatcaagccc attatcgggt gcgagatcta
      661 tgtggcaccg gagtcccgtt tcgataaaac cgcccccgcc ggacagaatg aggaggcatc
      721 ctatcacctc atcctccttg cccggaacag gcagggctac aagaacctcg tcaagctcgt
      781 cacggccgcc tacctcgagg gcttttacta caaaccccgg atcgacaagg accttctgcg
      841 ggctcacagc gaagggctca tcggcctttc ggcctgcctc ggaggcgaga tcccttccct
      901 cctgatccag ggccggtacg aggatgcaaa gaggaccgcc cttggatatg aacagatcct
      961 gggcaaggaa aatttctatc tcgaactcca ggacaacggc atccccgaac aggaacaggt
     1021 caaccgggag ctcatcaggc tcaacaagga cacgggcatc cccgtcgtcg ccacgaacga
     1081 ctgccactac ctcgacatgg aggaccacaa ggcgcacgac gcactgctct gcatacagac
     1141 cggcaagatc gtcacggacg cgaaccggat gaggttctct tccgagactt tttacatgaa
     1201 gacgcccgaa gagatgaaga agtccttttc cttcatcccc gaggccgtaa caaacaccgt
     1261 caagatagcg gagcgctgca atctcgacct cgagctgggc aagtaccatc tcccccactt
     1321 tcccgtcccc gaaggctaca ccagggaatc gttcatggcg gagctggcgc ggaaaggcct
     1381 cgaagagcgg ttcgtcgaga tcgaggccgt gcgcggcccc ggcagcatcg accgggctaa
     1441 atataaagac cgtctcgagt tcgagatcca gatgatcgac aagatgggct tcgccggcta
     1501 tttcctcatc gtatccgact ttatccgtta tgccaaggaa cacggtatcc ctgtcggtcc
     1561 cggccgcggc tccgctgcgg ggagcctggt cgcttactcg ctccgcatca ccgacctcga
     1621 ccccatcccc tacaacctcc tgttcgagcg gtttttgaac cctgaacgca tcagcatgcc
     1681 cgacatcgac gtggacttct gccaggaccg gcgcgacgag gtcatccggt atgtcacgga
     1741 aaaatacggt caggaccacg ttacccagat catcacttac ggaaccatgc tggccaaagg
     1801 cgtgatacgc gacgtgggcc gtgtcctcga catcccctat gccgaggtgg acaaggtcgc
     1861 aaagctcgtc ccgaacaccc tcggcatcac gctcgagaag gccatggagc aggagccaag
     1921 actcaaagag atcggcaaaa aggacccgcg gatggcggag ctcatggaga tcgccctggc
     1981 cctcgaaggc caggtgcgcc acgcatcgaa gcacgccgcc ggcgtcgtca tatcccagga
     2041 gcctctcacg gagtacgtcc cgctcttcaa gacaccgaag gacgaggtca ccactcagta
     2101 tgacatgaag ggcgtcgaga agatcggcct cgtgaagttc gatttcctcg gacttcggaa
     2161 cctgacggtc atccagaagg ccgaggacct gatcaaccag cgcctccaga aagcacctct
     2221 tgccggcacg gatccgttct cggtcagccg ggtccccctg gacgacgcta agaccttcga
     2281 actcctcggg cgcggggaaa ctgccggcat ctttcagctc gaatcctcgg gcatgcgcga
     2341 catcgtcatc aagatgaaac ccggggtgtt tgaagacctg atcgcgctcg tggccctcta
     2401 ccgtcccggc ccgctgggga gcggcatggt cgatgatttc atcaagcgca agaaaggctc
     2461 gacaaagatc acttacgagc ttccgcagct cgaaccgatc ctcaaggaca catacggcgt
     2521 gatcgtgtac caggagcagg tgatgcagat ctcgcgcacc ctggccggct actccctggg
     2581 ccaggcggac ctgctccggc gcgccatggg aaagaaggac cccgaggtca tggccaagga
     2641 gaaggttccc ttcctcaagg gcgcgggaaa acagggtatc gacctcaaga aggctgaggc
     2701 catcttcgat ctcatggcca agttcgccga atacggattc aataagtccc acagcgctgc
     2761 ctatgccctc atcacctacc agacagcata cctgaaggcg cattatcccg ttgaatacat
     2821 ggccgccctt ctgacctctg aagtcgccga cacggacaaa gtggtgaagt acatctacga
     2881 agcgcgccag atgaatatcg acatcctgcc gcctgacgtc aacgagagcc gctgggattt
     2941 cacggtcata gaggcccatg accgggaaag catcgaaccc ggcagcacca tcgggcgcat
     3001 ccggttcggc ctggccgcgg tcaagaacgt cgggatctcc gctatcgagg cgatgatcga
     3061 ggcccgcgaa acgaaaggca ccttcacgtc gctctgcgat ttctgcaaaa aggtggacca
     3121 gcgcagagtc aaccgccgcg tgatcgaggc cttgatcaag tgcggcgcct ttgacttcac
     3181 cggcgcccgg cgggctcaga tgatggaagc catcgatatg atgatggagg atgcggcaag
     3241 gcaccaggaa caggaagcca tcggtcagtt cagtatgttc ggctgcctgg acgacaagca
     3301 tgagccggcg ctgtcgaacg tgccggaatg gaaggagagc cagctcctag cctatgaaaa
     3361 agagagcatg ggattctaca tctcgggaca tcccctcgcc gcgtttcaag acgacatgaa
     3421 gcgctacgct accgccacca cggagaccct ggacagcgtt cccgacggca aggaagtgac
     3481 catctgcggc atcatctccg gtttgaagca gaagatcacg aaaaaaggcg ataagatggc
     3541 cattctgaac cttgaagacc ttaccggctc cgtggaaacc atcgtatttc ccgatctgta
     3601 ctcgacgtcc cagcacctgc tcctcacgga cacccccctc atcgtggccg gccagctcga
     3661 caagagcgaa cagggaaaca agatcaaggc cgtgcgtctt cacctcctgg ccgacgtgaa
     3721 gaagaaaggc acgacgcgca tcgacatcag cctctccgct accggcctga cccaggacga
     3781 tctgctcaag gtcaaggaca tcctgctccg ccatcgcggc gacatacccg tctatcttcg
     3841 tctcccgaac ccgacgcgca aggattcgct catctccgtg ggccgcgaca tccgcgtcac
     3901 tccctctgaa aggctcgtcg ccgagataga gtcgg
//
LOCUS       DASSLD010000337         3619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_501109,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000337 DASSLD010000000
VERSION     DASSLD010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501109"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFK23_06085"
     CDS             <1..237
                     /locus_tag="VFK23_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET7318686.1"
                     /translation="TLFDDRTKIEKMARIYESVDRIAKKFGKHSVQHAASLPTKLQAQ
                     HEGERGDVAVRRAILFKGENRRQRLRLPMLHVKV"
     gene            711..1271
                     /locus_tag="VFK23_06090"
     CDS             711..1271
                     /locus_tag="VFK23_06090"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET7318687.1"
                     /translation="MNKDLALEKIAEEIRACRLCKKGGLGKAVPGEGNADARIIFIGE
                     APGKEEAKAGRPFIGRSGKLLRKMIREAGIDEAEVFITSPVHYLPSAGTPTKEMIIHG
                     REHLLEQLSIIKPDIAVLLGNSACIAMLDRKIEISKIHGTLLEKDGRKYFITFHPAYA
                     MRFPDGRRGFIRDFSKLKKIIMASQP"
     gene            complement(1326..1595)
                     /locus_tag="VFK23_06095"
     CDS             complement(1326..1595)
                     /locus_tag="VFK23_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318688.1"
                     /translation="MPLYLIKTRHTLKNCLAALDEQLTHDVQSLHEFVYTCGEGEHAG
                     YAIVEANNRSEAMNIVPEPLRGEASVLKVDRFSPEDIRSFHAKAS"
     gene            complement(1614..2423)
                     /locus_tag="VFK23_06100"
     CDS             complement(1614..2423)
                     /locus_tag="VFK23_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HET7318689.1"
                     /translation="MNTRMDTMKHVFMPEGALASYLAPAGLALAVLAGIVEAFAGFGS
                     RWGWWHFTTGFAILRWTVVGGLAAALISLAGGIALRHAAQRAALLMSAAGILIGLVVA
                     GIPWSWMRTAQEVPRIHDITTDTVNPPRFDAVLALRKEAANPAEYGGPVVAAQQREAY
                     PDIAPVLLPVAQSTAFETALRAAKDMGWQIVSADEREGRIEATATTFWFGFKDDVVVR
                     VVPMPGGSRVDVRSVSRAGVSDVGTNAQRIHAFLHKVARMTSVDSTYVIPF"
     gene            2603..3415
                     /gene="lgt"
                     /locus_tag="VFK23_06105"
     CDS             2603..3415
                     /gene="lgt"
                     /locus_tag="VFK23_06105"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET7318690.1"
                     /translation="MIPYPHINPEIVRIGPVALRWYGMMYAAGFAASFLLVRHQIRKM
                     DREGKEEKRQGAAFPQGFLDSLYTYLILGLVLGARLGYVLFYDLSTYLRNPAEIFAVW
                     HGGMSFHGGMIGTILAGWLCCRKFKVDFWKTADIVIVTAPIGLGLGRLGNFINGELYG
                     RVTNVPWAMVFPSGGPLPRHPSQLYELFLEGVFLFVVLWLLKDRPHRAGTVTASFLML
                     YGVVRTIAEFFREPDAQLGFIAGPFTMGQVLSAITFAAGVVLYILRRKEATV"
BASE COUNT          819 a         1015 c          988 g          797 t
ORIGIN      
        1 acgctcttcg atgacaggac gaagatcgag aagatggcga ggatctacga gagcgtggac
       61 cgcatagcaa agaagttcgg caagcactcg gtccagcacg cagcgagcct gcccacaaaa
      121 cttcaggccc agcacgaagg ggagcgggga gacgtggcgg tcagacgggc gatccttttc
      181 aagggagaaa acaggaggca gaggctgagg ctgccgatgc tgcacgtgaa ggtgtagagg
      241 tcgagcgcgg gagggtgaaa ataaaaaagg cgtcaaactt ggaaccgact ctggtttttc
      301 cgttgcacct caggagagat acactaaaga tacgttttgc ttttccatcc gctttcgcgt
      361 acgttcgagc attccgtttc tttcttgtat cctccttcgg cttttaaggc tccagcttcg
      421 gtttttcatg atcctttgac ctgggagcag ttttggattt tcatcttctt ctgcttgccg
      481 gtttttgaat catttactcc ggtgctcttg ccttcaacgg aaaccttatt atctttgtta
      541 ctgccggttc cgtgtaacgc ctttaacgta ataataccac ttctttctaa atttgtcagc
      601 gtcctgtaaa tttttttagc aagtttataa atatacgaaa aaagttttag tcccgggaga
      661 gatgccggaa ataggataca atagattgtt gaaaggcccg tcgaccagtt atgaacaaag
      721 acctcgcctt ggaaaagatc gccgaagaaa tccgcgcctg ccgcttatgc aaaaagggcg
      781 gcctgggcaa ggccgttccc ggagagggaa acgctgatgc gagaatcatc ttcatcggcg
      841 aggcgccggg aaaggaagag gcaaaagctg gacggccttt cataggccgg tcgggcaagc
      901 ttctgcggaa gatgatccgt gaggccggga ttgacgaagc tgaggtcttc atcaccagcc
      961 cggtccatta ccttccttcg gccggcacgc caacgaaaga aatgatcatc cacggcaggg
     1021 agcatctcct ggaacagctc tcaatcatca agccggatat cgccgtgctc ctcggcaatt
     1081 cggcgtgcat cgcgatgctc gaccgaaaga tagaaatttc caaaatacac ggcactcttc
     1141 tcgaaaaaga cggcagaaaa tatttcatca cctttcatcc cgcctatgcc atgcgatttc
     1201 ccgacgggag gagagggttc atccgggact tctccaaatt aaaaaaaatc atcatggctt
     1261 cccagccatg attgacacct tcgcgtatct cttatcgccc gcccatcgca gttgtcacac
     1321 gattcctatg acgccttggc gtggaacgat ctgatatctt ccggtgaaaa cctgtcgact
     1381 ttcaataccg aggcctctcc gcggaggggc tcgggaacta tgttcatggc ctcgctgcgg
     1441 ttgttcgcct ccacgatggc ataaccggcg tgttcgcctt cgccgcaggt ataaacaaac
     1501 tcatgaaggc tctgaacatc gtgcgtcaac tgctcatcga gtgcggcaag acagtttttc
     1561 aatgtatgcc tggtctttat cagatagagc ggcatcttcg caccttcttc ccttcagaag
     1621 gggatgacgt acgtgctgtc gacgctggtc atcctcgcta ccttatgcag gaaggcatgg
     1681 atcctttgcg cattggtccc cacgtcgctc acgcccgcgc gggaaacgga gcggacatcg
     1741 accctgctcc ccccgggcat ggggacgacg cggacgacga catcatcctt aaacccgaac
     1801 cagaaggtcg tcgccgttgc ttcgatgcgc ccttcgcgtt cgtccgcaga gacgatctgc
     1861 cagcccatgt ccttagccgc gcggagcgcc gtctcaaagg cagtggactg cgcaaccggg
     1921 agcaggaccg gcgcgatatc cggatacgct tcccgctgct gcgcggcaac gacaggacct
     1981 ccgtattccg ccgggttggc cgcttccttc ctcaaagcca ggaccgcatc aaagcgcggt
     2041 ggattgaccg tatcggtcgt gatgtcgtgt atccgcggca cctcctgcgc cgtacgcatc
     2101 catgaccatg gaatgcctgc aacgacgagc ccgatcagaa taccggcggc ggacataagc
     2161 agggccgccc gctgcgctgc atgtctcagg gcgatgcccc ctgcaagaga gatgagggct
     2221 gcggccaggc cgccgacaac ggtccagcga agtatcgcga acccggtcgt gaaatgccac
     2281 catccccacc tgcttccaaa gcccgcaaag gcctcgacaa tgcccgcgag cactgcaagg
     2341 gcaagccccg ccggagcgag ataggacgcc agggcgcctt cgggcatgaa gacatgtttc
     2401 atggtatcca tacgagtgtt catgacttac ctccttcaac aggaaacagg acaaaacaaa
     2461 ttctatttat attctagcac ctcatcgttt tctgggaacg ggaccgcacg ccaaacacat
     2521 aagacctttg tatgcatctg ccgtatgctg tataattgat aaagaaaaaa catcccctat
     2581 gacggcagaa agaagaacgt cggtgattcc ctaccctcac ataaatccag aaatcgtgcg
     2641 catcggcccc gtcgcccttc gctggtacgg gatgatgtac gcggcgggat tcgcggcgtc
     2701 ctttctgctg gtcagacatc aaatcaggaa gatggaccgg gagggcaagg aggagaaacg
     2761 gcagggtgcc gcgtttccgc aggggttcct cgactctctc tacacctatc tcatcctggg
     2821 cctcgtcctg ggcgcgagac tgggatatgt cctgttttac gatctgtcaa cctacctgag
     2881 aaacccggct gagatttttg cggtatggca cggcggaatg tctttccacg gaggcatgat
     2941 cggcacaatc ctggcgggat ggctctgctg ccggaaattc aaggtggact tctggaagac
     3001 tgccgacatc gtgatcgtga ctgcccccat cggactcggc ctcggaaggc tggggaattt
     3061 catcaacggt gagctgtacg gcagggtcac gaacgtgcct tgggcgatgg tgtttccttc
     3121 gggggggccg cttccgcgcc acccgtccca gctttacgag cttttcctgg agggggtttt
     3181 cctgttcgtc gtcctgtggc tcctcaaaga cagaccgcac cgggcaggca ctgttacggc
     3241 gtcttttctc atgctctacg gcgtcgtgcg cacgatcgcc gaattcttcc gggaacccga
     3301 tgcgcagctc ggtttcatcg ccggcccctt caccatgggg caggtcctga gtgcgatcac
     3361 ctttgcggcg ggagtagtcc tgtacatctt acgcaggaaa gaggcaaccg tatgagcggg
     3421 ggccaccgca ctaatggtcc cgtaaaaaac cgtcattcga gcgtgcttct aagcctgccc
     3481 cggcatgcct gaagccgggg ggaatcgtat tgccacatta catactgaac agaagcgatc
     3541 ccggatgagc cggaatgaca tcaaaaggag aacttcacgt ttttttatga cttcatctgt
     3601 tatttaaacg aaagggagg
//
LOCUS       DASSLD010000338         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_612147,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000338 DASSLD010000000
VERSION     DASSLD010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612147"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="VFK23_06110"
     CDS             complement(<1..1384)
                     /locus_tag="VFK23_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET7318691.1"
                     /translation="MATLTAREIDIILDSTHDGLIAVDRSGIVTLFNRAAERITGLRA
                     EMVLGRPAIKVIPNTRLHVILAEGKSEINQEQRLNDVVIVTNRVPVRGEDGEIVGAVA
                     VFRDITELRSLADKVTGLWNVRSLLEAVIESTSDAISVADERGNTIIVNPAYTRITGL
                     SKDAVIDKPVTVDIAEGESMHLKVLTTGMPVRNVRMKVGPAKKEVIVNVAPIRIDGKI
                     RGSVGTIQDISEIMALTSELAQAKKLIRRLEARYTWDDIVGTGVILVAAKEQAMRAAG
                     TPATVLLRGESGTGKELFAHAIHNASGRSKGQFVRVNCAALPESLLESELFGYEEGAF
                     TGAVKGGKRGLFQEAHGGTIFLDEIGDITLPLQKKLLRVLQEKEVMRVGSTVPISVDA
                     RVIAATNADLEQKIKDGSFREDLYYRLNVLPIRIPPLRKIADDIPRVAKHLLVRLNQD
                     YGRQVEQIADE"
     gene            complement(1491..>2017)
                     /locus_tag="VFK23_06115"
     CDS             complement(1491..>2017)
                     /locus_tag="VFK23_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HET7318692.1"
                     /translation="AVLSITRGRTLVLFTSYGQLRKSAEALRREMSGLGFLCQGEMPA
                     YRLIEQFKGVPDSVLMGTASFWQGIDIPGDALQCVVIAKLPFAVPDDPIVESRMERLK
                     NPFYEYQVPQATLLFRQGFGRLIRTKTDRGAVAVLDSRIMTQNYGRSFLKSIPKCRVT
                     DKREEFRDFFGELR"
BASE COUNT          332 a          627 c          633 g          425 t
ORIGIN      
        1 cctcgtccgc gatctgctct acttgcctcc catagtcctg attcagccgc acgagcagat
       61 gtttcgcgac ccggggaatg tcgtcggcga tcttccgaag gggcggtatc cggatcggga
      121 gcacgttcag gcggtagtag aggtcctcgc gaaaagagcc gtctttgatc ttctgttcca
      181 ggtcggcgtt ggtcgcggcg atgacccgcg catccacgga aatcggcacg gtgctgccga
      241 ccctcatgac ctctttttcc tgcagcaccc gaaggagctt cttctggagg gggagcgtga
      301 tgtcgccgat ctcgtccagg aaaatggtgc ccccgtgggc ctcctggaag aggccgcgct
      361 tgccgccctt caccgcgccg gtgaaggcgc cctcctcgta gccgaagagt tcgctctcga
      421 gcaggctctc gggcagggcc gcgcagttca cgcgtacgaa ctggcccttg cttcttccgc
      481 tcgcgttatg gatggcgtgg gcgaagagtt ccttgccggt cccgctctcg cctcgaagga
      541 gcacggtagc cggcgtcccg gcggcgcgca ttgcctgctc cttggcggcc acgagtatca
      601 cgcccgttcc cacgatgtcg tcccaggtat agcgcgcctc gaggcggcgg atgagtttct
      661 tggcctgggc cagctccgag gtcagggcca tgatctcgga tatgtcctgg atcgtcccga
      721 cgcttccgcg gatcttcccg tcgatacgga tcggggcgac gttcacgatc acctcttttt
      781 ttgcgggccc gaccttcatc cggacgtttc tcaccggcat accggtcgta agcaccttga
      841 ggtgcatgct ctcgccctcg gcgatgtcca cggtcacggg cttgtcgatg accgcgtcct
      901 tcgagagccc ggtgatgcgc gtgtacgcgg gattgacgat gatggtgttg ccgcgctcgt
      961 ccgccaccga gatggcgtcg gaggtggatt cgatcaccgc ttcgaggagg ctccgcacgt
     1021 tccagagacc cgtcaccttg tccgcaaggg agcgaagctc cgtgatgtcg cggaacacgg
     1081 ccacggcgcc gacgatctct ccgtcctcgc cgcggaccgg cacccggttt gtgacgatga
     1141 cgacatcgtt caggcgctgc tcctggttga tttcggattt tccttcggcg agaatgacgt
     1201 gaagtcgtgt gttgggaatg accttgatcg cggggcggcc cagcaccatc tcggccctga
     1261 ggccggtgat ccgctcggcg gcccggttga acagggtgac gatgcctgag cggtccacgg
     1321 cgatcaaccc gtcgtgggtg gagtcgagga tgatgtctat ttcacgcgcg gtaagggtgg
     1381 ccatggaatg cacctcgcgg aattgtactg tatctcacgc tctttggcaa tagcgaccac
     1441 gggtagtcac gcagagagat gcgaaaaata gcgccctttc gttcgtgatg ttacctcaat
     1501 tccccgaaaa aatccctgaa ctcttccctc ttgtcggtga ccctgcattt cgggatggac
     1561 ttgaggaatg atcttccgta gttttgtgtc atgatgcggg agtccaggac cgcgaccgcg
     1621 cccctgtccg tcttcgtccg gatcagcctg ccgaacccct ggcggaacag gagcgttgcc
     1681 tgcgggacct ggtattcgta gaagggattc ttgagcctct ccatgcgcga ttcgacgatg
     1741 ggatcgtcag gcacggcaaa gggaagtttc gcgatcacca cgcactggag cgcgtcgccg
     1801 gggatgtcga tcccctgcca gaaggacgcc gtgcccatga gcacggaatc ggggacgccc
     1861 ttaaactgct cgatcaggcg gtaggcgggc atctctccct ggcacaggaa gccgaggccg
     1921 ctcatctccc gccgcagagc ctcggcgctt ttccggagct ggccgtagct cgtgaaaagc
     1981 accagcgtcc ttccccgcgt tatggacaga acggccg
//
LOCUS       DASSLD010000339         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_984893,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000339 DASSLD010000000
VERSION     DASSLD010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984893"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..662
                     /locus_tag="VFK23_06120"
     CDS             372..662
                     /locus_tag="VFK23_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318693.1"
                     /translation="MRTKKEEYIDKTAKQLKEWSTKIDELESRAAKASSEVKTGYKAL
                     MKDTKKKRDALSKELHKLGDAGGDAWDALKKGVEAASKDLKHAIEAARDKFK"
     gene            849..>2162
                     /locus_tag="VFK23_06125"
     CDS             849..>2162
                     /locus_tag="VFK23_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7318694.1"
                     /translation="MSNKLFIILAATVTAVALGFWFVLRPDLPLVNESLHSTLEAVES
                     IAALLMALFLLDRKEEDASGNSALLALGFLAMGILTAAHAASSTGNQFVFLRATASLA
                     GGLGFGLVWLPARYKALLDRAWLLWSVITGSLIICVAAFLFPAAFPAMVHGARFAAPA
                     VVINLLAGMLFVAGSVRIMITVYRSNALEDQLFSLIGMFFGLSGLTFQYAVLWSEGWW
                     YWHALRLIGSLLVLGLLVHRHLQMVATLKTSLLERKKAEMSLRRSYELTKTIIDSMND
                     AVSLLDVRDFRIIGVNSAFLKEYGYTDETEIVGKRCYEITHRQPDVCDVPDGMCPLAV
                     TVRTGKHAAADHVHYDGRGEKIFVEVSTSPIKDEDGKVIQVVHVAKYITERKRAEEER
                     ELLLADIARSNKDLEQFASVASHDLKEPLRMVSAYVQRLEKKYKGR"
BASE COUNT          491 a          583 c          636 g          452 t
ORIGIN      
        1 ctttatattc aggtttcctt gtcccgctgc cgggccgata cctcgccgga gcacggcaac
       61 ccggggaaac tcaaggtagt gttaaaaaat tttatgaaaa gcaaaaccat ttaaccacag
      121 aggtcgcaga gggaaaggaa ggcaagagtc agaagattca ggctttggtt taaaaccgcg
      181 aatgtcgcaa attagcgcga atagactcga aatcattagc gtatgttcgc gttcattagc
      241 ggttaaaaca aggcatcata gccgctgcag tcctcgcctc ttacccactg cgccctcggg
      301 agcctgcccc cgcaggttaa gcggggggtg aaagattttg actatacgaa gctcaacaga
      361 aggaggtcgg catgagaacg aaaaaagaag agtatatcga caagacggcg aagcagctca
      421 aggagtggtc caccaaaata gacgagctcg agtcacgggc agccaaggcc tcgtccgaag
      481 tgaaaaccgg ctataaggcg ctcatgaagg atacgaagaa aaaacgcgac gcgttgtcga
      541 aagagcttca taagctcggg gatgcgggcg gcgatgcctg ggacgccctg aagaaaggtg
      601 tggaagctgc ttccaaggac ctaaagcatg cgatcgaggc tgcgcgggat aagttcaagt
      661 aagcggctca ggcggcagct cccgattcat ggaaagtcgc cgccacccgg acggcggaat
      721 tgtcatttgc cgctattttc cggtcatttt gtccataaga cgcgaaagca gacccggtcg
      781 cgctcggtcg gcatctgcgc ttcccgcgga ttataccgca cgaactactc tgaagttgcc
      841 ggggaacaat gagcaataaa ctattcatca ttcttgccgc aacagtcacc gcggttgcgt
      901 tgggattctg gtttgttctg cggccggatc tgccactggt aaatgaatcc cttcacagca
      961 ccctggaagc cgtggagtcg attgcggcgc ttcttatggc gctcttcctg ctcgaccgga
     1021 aggaagagga cgcttccggc aacagcgctt tgcttgccct gggatttctt gcaatgggca
     1081 ttctgaccgc ggcccatgcg gcttcaagca cggggaatca gtttgtcttc ctgagggcga
     1141 cggcaagcct ggccggcggg ctcggattcg gcctggtctg gctgccagcg cggtacaagg
     1201 cgctccttga ccgggcatgg ctgctctggt ccgtcataac gggctcgctc atcatatgcg
     1261 tcgccgcgtt tctttttccc gcggcatttc cggcgatggt tcacggggcc aggttcgccg
     1321 ctcccgcagt cgtcatcaac ctccttgccg gcatgctctt cgttgcgggc tcggtgcgca
     1381 tcatgatcac cgtgtaccgt tcaaatgccc tcgaagacca gctgttcagc ctgatcggaa
     1441 tgttcttcgg cctttcgggt ctgacttttc aatacgcggt gctgtggtcg gaaggctggt
     1501 ggtactggca tgccctgcgg ctcatcggct cgctgctggt cctgggttta ctggttcatc
     1561 ggcatctgca gatggtcgcc acgctgaaaa cgtcccttct tgaacggaaa aaggcggaaa
     1621 tgtcgctcag gcgttcctat gagctcacga agaccatcat cgacagcatg aacgacgcgg
     1681 tctcgctcct cgacgtccgc gatttcagga tcatcggggt gaacagcgcc ttcctgaagg
     1741 aatacgggta taccgacgag acggagatcg tgggaaagcg ttgttatgaa ataacccacc
     1801 gacagccgga tgtctgcgac gtcccggacg gcatgtgccc gctcgcagtg actgtaagga
     1861 cggggaagca tgctgctgct gaccatgtac attacgacgg ccggggggaa aagattttcg
     1921 tcgaggtctc gacctcaccc ataaaagacg aggacgggaa ggttatacag gtggtccacg
     1981 ttgcaaaata cattaccgaa cgcaagcggg cggaagagga acgggagctg ctgcttgcgg
     2041 atatcgcgcg ctccaacaag gacctcgaac agttcgcctc cgtcgcctct cacgacctca
     2101 aggaaccgct cagaatggtg tcggcgtacg tccagcgtct ggagaagaag tataaaggcc
     2161 gg
//
LOCUS       DASSLD010000340         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_668194,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000340 DASSLD010000000
VERSION     DASSLD010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668194"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..972)
                     /locus_tag="VFK23_06130"
     CDS             complement(151..972)
                     /locus_tag="VFK23_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HET7318695.1"
                     /translation="MKTVIFTDLDGTLLDPKTYSFDEARLALDEIRKRNIPLVLCSSK
                     TRAEIEVYRKHLQNSDPFIVENGAATFIPTGYFRHPTGGVVREDYVVTTFGTPYRAVR
                     EALVRLRENMGIAVKGFGDMTVEEIAAVTELSYDEAALARIREFDEPFIFKSEPDERF
                     LRAIEEVGLHWTRGRLYHIMGDHDKGKAVRLLKRWYESERGKLVTIGLGDGFNDLPLL
                     AAVDHPVLIRKEDGSFDPRVDFPGLIKAKGIGPEGWNRAVLDLLSKNDKVVTRRI"
     gene            1136..2272
                     /locus_tag="VFK23_06135"
     CDS             1136..2272
                     /locus_tag="VFK23_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET7318696.1"
                     /translation="MFGKHVTLFRLLGFEVRVDLSWLVLAVLITWSLAVGVFPYYNEG
                     FARATYWWMGAIGAAGMFFSILFHEFCHSLVARRFGLLMRGITLFIFGGVAEMEDEPP
                     SPKAEFFMAVAGPLSSVVLGVAFYAAAKLTEGAAAVVPVQAVLEYLAMINWVLAAFNL
                     IPAFPLDGGRVLRAALWQRKKNLRWATRIASRIGSGFGMFLIFVGVANFLLGNIIGGI
                     WQLMIGMFLRTAAESSYRQVVYRKALEGETVERFMNPLPVTVPASISIRELVEAYIYR
                     YYFKMFPVLDEDRLLGCISTRQVKEIPREEWDRWTVGDRLDRCGDNNTISPGAGAMQA
                     LSLMGKTGNSRLMVVKDGRLVGIVSLKDIMGYLSTKMELGDEKE"
     gene            complement(2301..>2576)
                     /locus_tag="VFK23_06140"
     CDS             complement(2301..>2576)
                     /locus_tag="VFK23_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7318697.1"
                     /translation="RRIEALGILHSEGIPTFVMIAPLLPGAEGLADLLKGKVDHVLID
                     RLNYHYADRALKEHGLQWAGEAGFFAQKGEELRDSFEKQGIACRMLF"
BASE COUNT          519 a          750 c          737 g          570 t
ORIGIN      
        1 caccgtccgt ccttccgccg gaaaaagtga tacaattttg ataatctcat aaaagtcaaa
       61 atctaccgct gagacgccga gacgccgaga aaaaaattag taaattagtc aaattggatg
      121 gtttcctccg cgccctccgc gactctgcgg ttaaatccgc cttgttacga ctttgtcatt
      181 tttggataat aaatccagca ctgcccggtt ccatccctcc ggcccgatgc ccttcgcttt
      241 tataagaccg gggaaatcca cacggggatc gaagctcccg tcttccttcc ggatcaggac
      301 gggatggtca acggcggcga gaagcggcaa gtcattgaat ccgtcgccca ggcctatggt
      361 aacaagttta ccacgttcgc tctcatacca tcgcttcagc aaccggaccg cctttccctt
      421 gtcatgatcg cccatgatgt gatacagcct gccccgggtc cagtgaagac cgacctcctc
      481 gatcgccctc agaaagcgct cgtccggttc actcttaaaa ataaaaggtt catcgaactc
      541 ccgtatgcgc gccagggccg cctcgtcata tgaaagctcg gtcactgcgg caatctcctc
      601 aacggtcatg tcgccgaagc ccttcacggc gatacccatg ttttcacgaa gacggacgag
      661 cgcttccctg acagcacggt aaggtgttcc gaaggtggtg acgacgtaat cttcacggac
      721 aacgcctccc gtgggatgcc tgaaatagcc agtcggtatg aaagtggcag cgccgttttc
      781 cacaataaag ggatcagagt tctgcaggtg cttccggtac acctctattt ccgcccgtgt
      841 tttgctcgaa cagagtacca gcgggatatt cctctttctg atttcatcga gggcaagccg
      901 cgcctcgtcg aaggagtacg tcttcgggtc gagaagcgtc ccgtcgaggt cagtgaagat
      961 gaccgttttc atgttactat tctaccatcg cgcgcggggc cgtgcctgct cggatctctc
     1021 tgatgtataa ttaaaataca caatgagcgc aacaacaaag atgttgcttg atgtaccgaa
     1081 ggtgtacttc cggcggaaag catcggcaat gaaagggagc ttcgggaggt agacggtgtt
     1141 cggaaagcac gtcacgctgt ttcgccttct cgggttcgag gtccgcgttg acctgagctg
     1201 gctggtcctg gccgtgctca tcacctggtc gctggccgtc ggcgtgttcc cttactataa
     1261 cgagggcttc gcgcgggcaa catactggtg gatgggtgca atcggcgccg ccggcatgtt
     1321 cttttcgatc ctcttccatg agttctgcca ctccctcgtc gcccggcgtt tcggtcttct
     1381 catgcggggc atcaccctgt tcatcttcgg cggcgtggcg gaaatggagg acgagccccc
     1441 gagcccgaaa gcggagttct tcatggccgt ggcgggcccc ctttccagcg tggtcctcgg
     1501 ggtcgcgttc tatgccgcgg caaagctgac ggagggcgcc gcggccgtcg ttccggttca
     1561 ggccgtgctg gaatatcttg ccatgatcaa ctgggtcctg gccgccttca accttattcc
     1621 cgcgttcccc cttgacggag gccgcgtgct gcgcgccgcg ctgtggcagc ggaaaaagaa
     1681 cctgcgctgg gcaacgcgca tagcatcacg gatcgggtcg ggcttcggca tgttcctgat
     1741 attcgtcggc gtggcgaact tccttctcgg caacatcatc ggcggcatct ggcagctcat
     1801 gatcggcatg ttcctccgta ctgcggccga gtcgtcctac cggcaggtcg tataccgcaa
     1861 ggcgcttgaa ggcgagactg tggagcggtt catgaaccct ctgcccgtga cggtgccggc
     1921 gtcgatctcg atcagggaat tggtggaagc ctacatttac cgctactatt tcaagatgtt
     1981 tcccgtcctg gacgaggaca ggcttctggg gtgcatctca accaggcagg tcaaggagat
     2041 cccgcgggaa gagtgggacc gatggacggt gggcgatcgg ctcgaccgtt gcggggacaa
     2101 taataccatc agtcccggcg caggggccat gcaggccctc tcgctcatgg ggaaaacagg
     2161 caacagcagg ctcatggtgg taaaagacgg acggcttgta gggatcgtct ccctcaagga
     2221 catcatgggc tacctgtcca cgaagatgga gctgggagac gaaaaggagt aggagggacc
     2281 gcactgccgg caattcctga tcagaagagc attcgacagg cgatcccctg cttctcgaac
     2341 gagtccctca gctcttcgcc tttttgcgcg aagaagcccg cctcccctgc ccattgcaat
     2401 ccgtgttctt tgagcgccct gtccgcataa tggtaattca gccggtcgat gagcacgtgg
     2461 tcgaccttgc ccttgaggag gtcagccagc ccttcagcgc cgggcagcag gggggctatc
     2521 atgacaaagg ttggtatgcc ttcggaatga aggatgccga gcgcctcgat ccgacg
//
LOCUS       DASSLD010000341         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_774527,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000341 DASSLD010000000
VERSION     DASSLD010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774527"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1488
                     /gene="nuoF"
                     /locus_tag="VFK23_06145"
     CDS             217..1488
                     /gene="nuoF"
                     /locus_tag="VFK23_06145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HET7318698.1"
                     /translation="MERPLTQHINPGKEPLSLKDYEKAGGYQSVRKALAMTPQDVQNV
                     VKASNLRGRGGAGFPTGAKWSFVPMGPEARRPKYFVVNADEMEPGTFKDRLLLEGNPH
                     QLIEGVIASAYAIEAEQAIVFVRWAYKLATRRLNQAIADAYENGYLGKNILGSGYSLD
                     VRLHGSAGRYICGEETALLNALEGRRATPRAKPPFPQVCGLFGKPTIVNNVETVSNIP
                     HIVQNGPDWYKGLSKIADTGTKLYGASGRVRKPGIWELPMGVTVRELLEEHAGGMQKG
                     YQFRGVIPGGASTDFLVSEHLDVKMDFDSVMKAGSRLGTGTMIVLDDKTCPVGFVHNL
                     IKFFARESCGWCTPCRDGLPWIEKILRAMENGQGEPGDIDILAQQCRLLGPGNTYCAL
                     APGAVEPLQSAIKYFREDFERHIKEKKCPWK"
     gene            1650..>2430
                     /locus_tag="VFK23_06150"
     CDS             1650..>2430
                     /locus_tag="VFK23_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET7318699.1"
                     /translation="MITIFIDNKPYQVKEGQNLLNACLSLGFDLPYFCWHPAMHSVGA
                     CRQCAVKVFKDEKDTRGRLMMSCMTPVAEGLRLSINDAEARAFRSSVIEWLMLNHPHD
                     CPVCDEGGECHLQDMTVMTGHNYRRTRFRKRTHRNQDLGPFINHEMNRCIACYRCVRF
                     YRDYADGRDLDAFASHDNVYFGRAEEGALESEFSGNLVEVCPTGVFTDKTLKRHYTRK
                     WDLQTAPSVCVHCSLGCNTIPGERYGTLRRVLNRYNRQVNGY"
BASE COUNT          568 a          704 c          713 g          445 t
ORIGIN      
        1 catgatggtg gatgaggacc tgtacggccc ggttacggcg gagatgatgg aagaaatact
       61 gaaaaaatac gattaacccc tgatggatat gcattttcaa actggaaagc gaaaaaatta
      121 ctcaccgcga atttacgcga atagaaacag aatattcatc tcttcaattt tattcattca
      181 cgttcattag cgcccattcg cggttaaagg ggttttatgg aacgaccgct cacacaacat
      241 atcaacccgg gcaaagagcc cctctctctg aaggactacg agaaggccgg cggctaccag
      301 tccgtgcgaa aggctctcgc gatgacgccg caggacgtgc agaacgtcgt gaaggcgtcg
      361 aacctgcgcg gccgaggagg agcgggcttc cccacgggcg ccaagtggag ctttgtgccc
      421 atgggcccgg aggcgcggcg tccgaaatat ttcgtcgtga acgccgacga gatggagccc
      481 ggcaccttca aggacaggct cctgctcgag ggcaaccccc accagctcat tgagggggtg
      541 attgcgagcg cctatgccat cgaggcggag caggccatcg tcttcgtcag gtgggcctac
      601 aagctcgcga caaggcgcct gaatcaagcc atcgccgatg cctacgagaa tggctacctt
      661 ggaaaaaaca tcctggggtc cggctacagc ctggacgtgc gcctccacgg aagcgccggc
      721 agatatatct gcggcgagga gaccgccctg ttgaacgcat tggaaggaag acgggctacg
      781 ccccgcgcaa aaccgccctt cccccaggta tgcggactct tcggcaaacc aacgatcgtg
      841 aacaacgtcg agaccgtctc gaacataccg cacattgtgc aaaacggccc ggactggtac
      901 aagggcctga gcaagatcgc ggacaccggc accaagctct acggtgcgag cggcagggtc
      961 aggaagccgg gtatctggga acttcccatg ggcgtcacgg tgcgggaact gctcgaggaa
     1021 catgcgggcg gcatgcagaa gggctatcaa tttcgcggcg tcatccccgg gggcgcgtcc
     1081 acggactttc tcgtttccga gcaccttgat gtgaagatgg attttgattc cgtgatgaag
     1141 gcgggaagcc ggctcggcac cgggaccatg atcgtcctcg acgacaagac ctgcccggtc
     1201 ggtttcgtac ataacctgat aaagttcttt gcgcgcgagt cctgcggctg gtgcactccc
     1261 tgccgcgacg gtctgccgtg gatcgagaaa atcctccggg cgatggaaaa cggccagggc
     1321 gaacccgggg acatcgatat cctggcgcag cagtgcaggc tcctgggccc gggcaacact
     1381 tactgcgcgc tcgcgcccgg cgcggtcgag ccgctccaaa gcgcgatcaa gtacttccgg
     1441 gaagacttcg agaggcacat taaagaaaag aaatgcccgt ggaaataatt tagcggaaag
     1501 cgtatccact attgtcattc ctgctgcgaa acttgtcctc gaatgattca atcggggagc
     1561 aggaatccat ttttggatat aagaagccga tggattcccc cggatcaagt ccggggcagg
     1621 cttgcaagcc ccgaatgaca gcaaagacta tgatcacgat cttcatcgac aacaaaccct
     1681 accaggtcaa ggaaggccag aacctgctta acgcctgcct gtcgctaggc ttcgaccttc
     1741 cctacttctg ctggcacccg gccatgcact ccgtgggcgc gtgcaggcag tgcgcggtca
     1801 aggtgttcaa ggacgagaag gacacccgcg gcaggctcat gatgtcctgc atgacgccgg
     1861 tggccgaggg ccttcgcctt tcaatcaacg acgccgaggc aagggccttc cggtcaagcg
     1921 tgatcgaatg gctcatgttg aaccaccccc atgactgccc ggtctgcgac gagggcggcg
     1981 agtgccatct ccaggacatg acggtgatga ccggtcataa ttatagaagg acacgattca
     2041 ggaagcggac gcatcggaac caggacctcg gcccttttat caaccacgag atgaaccgct
     2101 gcatcgcctg ctaccgctgc gtcaggttct atcgggacta cgcggacggc cgggaccttg
     2161 atgcatttgc ctcgcatgac aatgtttact tcggaagagc tgaggaaggc gcgctcgaaa
     2221 gcgagttcag cggaaacctc gtcgaagtgt gtcccacggg cgtattcacg gacaagacgc
     2281 tcaaacgtca ctacacgcgc aagtgggacc tccagaccgc gccctccgtc tgcgtccact
     2341 gcagcctcgg ttgtaatacc atccccggcg aacggtacgg tacgcttcga agggtcctga
     2401 accgttacaa ccgacaggtg aacggatact
//
LOCUS       DASSLD010000342         3597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_979328,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000342 DASSLD010000000
VERSION     DASSLD010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979328"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..443)
                     /gene="rplM"
                     /locus_tag="VFK23_06155"
     CDS             complement(12..443)
                     /gene="rplM"
                     /locus_tag="VFK23_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET7318700.1"
                     /translation="MGTYSAKKEDIKRSWHVVDANGKTLGRLATVVASVLKGKTKPIF
                     TTHVDTGDFVIVVNADKIHLTGRKLDQKVYYSHSGFPGGLKSVTAGKLLKTKPEEVFK
                     KAVEGMLPKTRLGKQMLSKLKVYAGESHPHNAQQPVEMKIK"
     gene            complement(730..1065)
                     /locus_tag="VFK23_06160"
     CDS             complement(730..1065)
                     /locus_tag="VFK23_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318701.1"
                     /translation="MNTTISRKTAYIGAGAGLVLFALFGLMPGSLMGGAAGIGIAGWL
                     FGLPLQPALLSRIIVLASMLFGVLVAGIVIVTVTSTVGWLIGKVIEASIHAKEAGQEK
                     DLGKAEGRK"
     gene            1434..2234
                     /gene="rpsB"
                     /locus_tag="VFK23_06165"
     CDS             1434..2234
                     /gene="rpsB"
                     /locus_tag="VFK23_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET7318702.1"
                     /translation="MSTITMKELLEAGVHFGHQAKRWNPKMKKYIFGERNGIYIIDLQ
                     KTLKLFKEAYEFVRTSAGEGKDILFVGTKKQAQDAITEEAKRCGMYYVSNRWLGGMLT
                     NFTTIRKSIDRLKKIEKMKEDGTYDKLTKKEVAGLEKERTKLEKILSGIKSMSQPPSV
                     IFVIDPRKEQIAVQEANKLNIPVVAVVDTNCDPDNIDYVIPGNDDAIRAIRLMASKMA
                     DAVIEGRQAIAKEQGEAMEAAKAAGETQAVPVEPAAEEAEADVDEDVV"
     gene            2283..3167
                     /gene="tsf"
                     /locus_tag="VFK23_06170"
     CDS             2283..3167
                     /gene="tsf"
                     /locus_tag="VFK23_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET7318703.1"
                     /translation="MATISASAVKDLREKTGVGMMEAKKALEEAGGDFEKAVDVLRKK
                     GLSAAAKKSARVASEGMIASCVTGEGKTGVLIEVNSETDFVAKNEDFQKFAKGLAELV
                     ATKKPADVAALSKLDSGGESVEAKRNALVQKIGENIAVRRFVTYETSGRLAIYLHGNK
                     IGVMVDYSGGDEQLGKDLAMHIAAANPQFLNRETVPAEVLGRERAVYEAQAKESGKPA
                     AVIGKIIDGKLEKFYTDTCLVDQIYIKDPDGKLRIRDVLKKAGPNVVINRFVRYQLGE
                     GIEKKKESFAEEVAAQLK"
     gene            3267..>3597
                     /gene="pyrH"
                     /locus_tag="VFK23_06175"
     CDS             3267..>3597
                     /gene="pyrH"
                     /locus_tag="VFK23_06175"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003350165.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET7318704.1"
                     /translation="MTSPRYKRILLKLSGEALMGEQGYGIDHAVLDTITSEVKEVAGL
                     GVEIAIVIGGGNIFRGLSGTAKGMERASADYMGMLATVLNALALQNVLENKGIVTRVQ
                     SAIEMREL"
BASE COUNT          911 a          963 c         1005 g          718 t
ORIGIN      
        1 gttcctattc gttatttaat tttcatctcg acgggctgct gggcgttgtg cggatggctt
       61 tctcccgcat acaccttgag cttggaaagc atctgtttgc ccagcctggt cttgggaagc
      121 attccctcga ccgccttctt aaaaacttcc tcgggcttgg tcttgagcag cttgcccgca
      181 gtgactgatt tgagcccgcc ggggaatccg gagtggctgt aatagacctt ctgatcaagc
      241 ttccttccgg tcagatgaat cttgtccgca tttacgacga tcacgaagtc gccagtgtcg
      301 acgtgagtcg taaagatagg cttggtcttc cctttgagca ccgatgccac gacggttgca
      361 agcctgccga gggtcttgcc gttcgcatca acgacatgcc aacttctctt gatatcttcc
      421 ttttttgccg aataagttcc cataatttcc gccttgttga tatgttgatt attatcagga
      481 attgcgaata taaacaattt cacctgtttt gtcaagcgtt tttgtcaaat ggcgaaagga
      541 aaaaaattac caggcgggaa atatgaccgt agtgtttaaa aaaataattt aaatatcaat
      601 atattattaa gacttgtaga gaagaagtgc ccgtggaacg gtgcctgata cggattgcaa
      661 gatgtcgccc ccattaaaaa gggcagccct atgaggctgc ccctccgtct tctgaatata
      721 tcacggtagc tatttacgcc cctcggcctt gcccaggtct ttttcctggc ctgcctcctt
      781 tgcatggatg ctggcctcaa tgaccttgcc gatgagccat cccactgtcg aggtgaccgt
      841 cacgatgacg atgcccgcga ccagtacacc gaaaagcatg gacgctaaga caatgatccg
      901 ggacaagagg gcgggctgaa gaggcagtcc gaaaagccag ccggcgattc cgataccggc
      961 tgcgccgccc atcaggctgc cgggcatgag tccgaagagc gcgaacaaaa ccaggccagc
     1021 gcctgccccg atgtatgctg tcttgcgaga aatcgtcgta ttcatgtttg ctgcctcctt
     1081 tcatcgtctc ttgaaagggt aaattgcatc atccatgcca tatcatagct cattgatttt
     1141 aaaagaatag aatgtcagca atctttggca gaagtgagaa aaaaccggcg gttatatcaa
     1201 ggaatgcgaa aatgtccatc ctgctaagat cgtgacaaaa aacacaaggg aagatatttt
     1261 ccttgaaaac cagcgagctc tatggtaatt tattgaccgc ctttcttcaa tgaaaggcta
     1321 tttcacatta cacacgcgcc aggacttacg gcaggatgtc ccggtaccag gaaatcggga
     1381 tctgtggtga ggatgatcgg cgcggcggta aaaatctgaa ggagggacat ctcatgtcca
     1441 cgatcaccat gaaggagttg ctggaagcgg gggttcattt cggccaccag gccaaacgct
     1501 ggaaccccaa gatgaagaag tacatattcg gcgagcggaa cgggatatac atcatcgatc
     1561 tccagaaaac gctcaaactc ttcaaagagg cctacgagtt cgtccgcacg tctgccggcg
     1621 aggggaagga catcctcttc gtagggacca agaagcaggc ccaggacgcg atcaccgaag
     1681 aggccaagcg ctgcggcatg tactatgtct ccaaccgctg gcttggcggc atgctcacga
     1741 atttcacgac catccgcaag agcatcgacc ggctcaagaa aatcgaaaag atgaaggagg
     1801 acggcacgta cgacaagctc accaagaaag aagtagccgg cctcgaaaaa gagcgaacaa
     1861 agctggagaa aatactctcc ggcatcaagt caatgtcgca gccgccctcc gtgatctttg
     1921 tcatcgatcc gcgcaaggaa caaatcgccg tccaggaggc gaataagctg aatatccccg
     1981 tagtcgccgt ggtggatacg aactgcgacc ccgacaacat cgattatgtc atcccgggca
     2041 acgacgatgc catccgggca atccggctta tggcttccaa gatggccgat gccgtcatcg
     2101 aaggcaggca ggccatcgcc aaggagcagg gtgaggccat ggaggctgcg aaggccgcgg
     2161 gagaaaccca ggccgtgccc gtggagcccg cagctgagga agcagaggca gacgtcgatg
     2221 aggatgtcgt ttaagaaata ctcccaagac gcgttatgaa cggagaaagg ggtttttaag
     2281 caatggcaac aatatccgca agtgcagtaa aagacctgag agaaaaaacc ggcgtcggta
     2341 tgatggaggc gaaaaaggcc ctggaagaag ccggcggtga ctttgaaaag gccgtcgacg
     2401 tcctcagaaa gaaggggctc tccgcagcgg cgaagaagtc cgcgcgtgtg gcctccgagg
     2461 gcatgatcgc ctcgtgcgtc accggggaag gcaagaccgg cgtcctgatc gaggtgaaca
     2521 gcgaaacaga cttcgtggcg aaaaacgagg atttccagaa gttcgcgaaa ggactcgccg
     2581 aactcgtggc gactaagaag ccagctgacg tggctgcgct ctccaagctg gactcgggag
     2641 gcgagagcgt tgaagcgaag cgcaatgccc tcgtgcagaa gatcggtgag aatatagccg
     2701 ttcgccgctt cgttacgtac gagacctccg gcaggctcgc gatctacctc cacggcaaca
     2761 agatcggcgt catggtcgac tattccggcg gcgacgaaca gctcggcaag gacctcgcca
     2821 tgcatatcgc cgctgcgaac ccccagttcc tcaaccgtga aacggtcccc gcagaggtgc
     2881 tcggccgtga gcgcgccgtg tacgaggcac aggcgaagga atccggcaag cccgccgccg
     2941 tgatcgggaa gattatagac ggcaagctcg aaaagtttta caccgacacc tgcctcgtcg
     3001 accagatcta catcaaggac cccgacggca aactgaggat ccgggacgtc ctgaaaaagg
     3061 ccggcccgaa cgttgtcata aaccggtttg tccggtacca gctcggcgag ggcatcgaaa
     3121 agaagaaaga aagcttcgcc gaggaagtgg cggcgcagct caagtaaacc gaaccggcga
     3181 gggggcgacg ggggttatcg gcgcaaaagc caaggcccct tccccctttc gcccattcac
     3241 cctttcgccc attcgttttt ataattatga ccagtcccag atacaagcga attctcctta
     3301 agctctccgg tgaggccctc atgggcgagc agggatatgg catcgatcac gccgttctcg
     3361 acacgatcac gtccgaggtg aaggaagtcg ccggtctcgg ggtggagatc gcgattgtca
     3421 tcggaggcgg caacatcttc cgggggctgt ccgggactgc caagggcatg gaacgcgcga
     3481 gcgcggacta catgggaatg ctggccaccg tcctgaacgc cctggcgctc cagaacgtcc
     3541 tcgaaaacaa gggcatcgtt acccgggtgc agtcggccat cgagatgcgc gagctcg
//
LOCUS       DASSLD010000343         3936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_99668,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000343 DASSLD010000000
VERSION     DASSLD010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99668"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /gene="phnD"
                     /locus_tag="VFK23_06180"
     CDS             complement(<1..1521)
                     /gene="phnD"
                     /locus_tag="VFK23_06180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7318705.1"
                     /translation="MSRLFMTSWLALWMLCLSITAGSAREVRPSEAPPDSDAPAPLVF
                     ILPPVENVSVMYEKFLPLSEYLEKELSRPVVLRVAQNYQEAIESIGTGKADLSYLDPS
                     AYCEARHLHRIFPLVKAILNGSATYRSVIVARKDSGLRRIVEAKGKRFALGNVSSSSS
                     YLIPAVMFKEVGIGLSDFASVDYLEQEDRIALSVLARRHDIGGLSERVARKYEGDGLA
                     IINESEAIPQYTLCASANLPAPVRAAVRQALLAIGKSRNPALISAMKDIDGFAPAEER
                     DFDVVRVMIKNLTGKNYLEYGRNAIKVAILPLYSAITLFDRFDPLMRYLSQRTGAEFK
                     LVIPRDFEDFFDTVRNGSVQFSYSNPYIYIQLADHGYASAFANTVLESSGDIFRGIII
                     ARRESPIATVGDLRGKRVMVVSYKSAGGFLAQKLLLNEQGIDVFRELRLEEGKRQEEV
                     ILNVYRKNVDAGFVRESALDVLKEEIDLGQIKIVARTPYIANWPFASTRTADPKIVE"
     gene            1706..1933
                     /locus_tag="VFK23_06185"
     CDS             1706..1933
                     /locus_tag="VFK23_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HET7318706.1"
                     /translation="MIQTDKTLDIKGLAELRTETITGDTLEGMARGQVLTVVTTDRQA
                     RRKLTRLCEHLGYTLLDFREDKGNLYVQIRK"
     gene            1947..3602
                     /locus_tag="VFK23_06190"
     CDS             1947..3602
                     /locus_tag="VFK23_06190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7318707.1"
                     /translation="MDIGKIEQALLLSFCMLIVVPLLFIFRSFDHNTFTNWQWVFSGP
                     GMARVFLLIVPAIFCAYGVARLSLAGPRAIAFLFVLSLAAVLPLWSGPESVIDASRYF
                     IQAKAMKEYGISYFFKEWGRSIPAWTDLPLVPFLYGLLFSRAGEARAVIQAINSVLFA
                     LTAVLTFLIGKKLWDEDTGFRAGLLLLGVPYLLVQAPLLLVDVPTMFFLTLSVFAFLV
                     AIERGGFARTAAAALVICAAFFSKYSTWPMLALIPVIAAVRPGSDAKKTWIRTCAVLG
                     MSLALAGLVIALRLDFFREQFHLLWTYQRPGLGRWHEGFVSAFFFQTHPFIAGLALFG
                     TYQAVRKTDKNFLIPAWFFVITILLRIDRVRYLIPLLPLFVLMASYGLGAVRDGAARR
                     FICLSIAGTSMVVAWGAYLPFLAGTSPANLAEAGAFLNGLGCSPVEVYALPQKSSTGS
                     TFAAIPLLDYFTDAAFVSPQDWPVLPEDSARQVSSLRFTWEMRKPAYYAKRVAENSCA
                     VVLLSDEILDRPPPAYKGWSVKRFDLQSPMFKYQTIVTVLTRQ"
BASE COUNT          743 a         1176 c         1139 g          878 t
ORIGIN      
        1 ctcgacgatt ttcggatcag cggtccgcgt cgacgcaaag ggccagttcg cgatgtacgg
       61 ggttctcgca acgatcttga tctggcccag gtcgatctcc tctttgagca cgtcgagcgc
      121 cgattcccga acaaagcccg cgtccacgtt cttccggtag acgttcagga tcacctcttc
      181 ctgccttttt ccctcctcga gccggagttc ccggaacacg tcgatgccct gttcgttcag
      241 gagcagtttc tgggcgagaa aaccgccggc ggacttgtac gagacgacca tgacgcgctt
      301 tccccgaagg tccccgacgg tcgcgatcgg gctctccctg cgggcgatga tgatgccccg
      361 gaagatgtcg cccgatgact cgagtacggt gttcgcaaag gcggaggcgt acccgtggtc
      421 ggcgagctga atatagatgt aggggttgga gtaggagaac tgaacgctcc cgttccgcac
      481 ggtgtcgaaa aagtcctcga agtcccgggg gatgacgagc ttgaattcag cgcccgtcct
      541 ctgcgaaagg taacgcatga gcgggtcgaa ccggtcgaag agcgtgatgg cggaatagag
      601 cggcaggatc gcgaccttga tggcgttcct gccgtattcg aggtagttct tgcccgtcag
      661 gttcttgatc atgacgcgga ccacgtcgaa gtcgcgctcc tccgcggggg cgaacccgtc
      721 gatgtccttc atggccgaga taagcgccgg gttcctgctc ttcccgatgg cgaggagcgc
      781 ctgccggaca gcggcgcgga cgggggccgg caggttcgcg gaagcgcaga gggtgtactg
      841 ggggatcgcc tcggactcgt tgatgatcgc aaggccgtcg ccctcatact tgcgcgccac
      901 gcgctcgctc aaccccccga tgtcatggcg gcgggcgagg accgacaagg cgatccggtc
      961 ctcctgctcg agataatcca cggaagcgaa gtcggagaga ccgatcccta cttccttgaa
     1021 catgacggcc gggatgaggt aggaagaaga cgagcttacg tttcccaggg cgaagcgctt
     1081 tcccttcgcc tcgacgatcc gccgcagccc cgagtctttt cttgccacga tgacgctccg
     1141 gtatgtcgcc gaaccgttca ggatggcctt cacgagcggg aatatccggt ggaggtgtct
     1201 cgcttcacaa taggcggacg ggtcgagata ggagaggtcg gcctttcccg tcccgatgct
     1261 ctcgatggcc tcctggtaat tttgcgccac cctgaggacg accgggcggg aaagctcttt
     1321 ttcgagatac tccgaaagag gaaggaactt ctcatacatg acgctgacgt tctccacggg
     1381 cggaaggatg aaaacgaggg gagccggagc atcgctgtct ggaggcgctt cggacggacg
     1441 gacttcccgg gcggaccccg cggttatcga gagacagagc atccacaggg ccagccagga
     1501 agtcatgaac aggcgagaca tggcacatta tacgcacgga gaacaaaaca ttgcaacatc
     1561 caactggccc ctgacggccg aaacggaggg tcttggccgg aacgggccga tggcggcctt
     1621 tcgcacgggc aattcccgtg aattatctcg cggttagcag gctggcatca tccttgcttt
     1681 ttcttataag aataagggaa cagccatgat ccaaacggat aaaacgctcg acatcaaagg
     1741 cttggccgag cttcgcacgg agaccatcac cggggacacg cttgagggca tggcgcgggg
     1801 ccaggtcctg accgtcgtta ccacggaccg gcaggcaaga cggaaactga cgcgcctgtg
     1861 cgagcatctg ggctatacgc tccttgattt ccgggaagac aaaggcaacc tgtacgttca
     1921 gatccgcaaa taatcaagga gaagcggtgg atatcggaaa gatcgaacag gcgctgctcc
     1981 tctccttctg catgctcatc gtggtgccgc tcctcttcat ttttcgctcc tttgaccaca
     2041 acaccttcac gaactggcag tgggtatttt cgggaccggg catggcgcgg gttttccttc
     2101 tcatcgtccc ggcaatcttc tgcgcctatg gcgtcgcccg tctctccctt gcaggacccc
     2161 gtgcaatcgc ttttcttttt gtcctctccc tggcagccgt ccttccactc tggtccgggc
     2221 ccgagtctgt catcgatgcg tcccgttatt tcatccaggc gaaagccatg aaagagtacg
     2281 gcatctctta ctttttcaaa gaatggggca ggtcgatccc cgcatggacc gacctgcccc
     2341 tcgtgccctt tctctacggg ctcctttttt cccgggcggg agaggcccgg gccgtcattc
     2401 aggcgatcaa ctcggttctc tttgcgctta ccgccgtcct tacctttctt atcggaaaga
     2461 aactctggga cgaagacacg gggtttcggg caggccttct ccttttgggg gtcccctatc
     2521 tcctggtgca ggcgccactc ctgctcgttg acgttcccac catgtttttc ctgacccttt
     2581 ccgtctttgc attcctcgtg gcgatcgaac ggggagggtt tgcgcggaca gcggcagcgg
     2641 cccttgtgat ttgcgcggcc ttcttctcca agtattcaac atggcccatg ctcgccctta
     2701 tcccggtcat tgctgcggtc cggccgggca gtgacgcgaa gaagacctgg atcaggacct
     2761 gtgccgttct gggcatgagc cttgcccttg ccggcctcgt cattgcgctg cggcttgatt
     2821 ttttccgcga gcagttccat cttctctgga cctatcagcg gcccggcctc gggaggtggc
     2881 acgagggctt cgtctcggcc tttttcttcc agacccatcc ttttatagcg gggcttgcgc
     2941 tcttcgggac gtatcaggcc gttcgcaaga cggacaaaaa ttttcttatt cccgcgtggt
     3001 ttttcgtcat caccatcctt ctacggatcg acagggtccg ctacctcatt cctcttctgc
     3061 cgctcttcgt cctcatggcg tcgtacggct tgggcgcggt cagggacggg gcggcaagaa
     3121 ggttcatctg cctttccatc gccggtacat ccatggttgt cgcctggggg gcctatctcc
     3181 ctttccttgc cggcacgtct ccggcaaacc tcgccgaggc tggtgccttc ctgaacggcc
     3241 tgggctgcag tcctgtcgag gtctacgccc tgccgcagaa aagctcgacc ggaagcacct
     3301 tcgcggcaat cccgctcctc gattatttca ccgatgcagc gttcgtctcg ccccaggact
     3361 ggcccgtcct tcccgaagac agcgcaagac aggtatcttc cctgagattt acgtgggaaa
     3421 tgaggaagcc ggcctactac gcgaagcggg tcgcggagaa tagctgcgcc gttgtactcc
     3481 tgtccgatga aattctcgat cgtccgccgc cggcatacaa aggctggagc gtgaagcgat
     3541 tcgaccttca gtcgcccatg tttaaatatc agacgatcgt gaccgtgctt acgaggcaat
     3601 gactgtcccg aatgggacat cgagcccctt ttcgatgtcc cgaacgggac agcactcatc
     3661 cttcacgatg cgcgctctcc aggagcacgg catcccaact cattgtatcc atttcatatt
     3721 gattcgtgag ttcgtggggg gccatgctgg tacgacgaat gcaatagact gtcgacagag
     3781 cttaatacct catgaaggag gtaccgaggt ggaagaagcg gcaaaaaaga aaaagatatg
     3841 ggccctggtc ataaccggtt tttttgcgct ggtgagcgtc acgacattcg cttataacga
     3901 ctactatatc tatctttccg cgtacctctg gttcgg
//
LOCUS       DASSLD010000344         3618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_300162,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000344 DASSLD010000000
VERSION     DASSLD010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300162"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            413..2815
                     /locus_tag="VFK23_06195"
     CDS             413..2815
                     /locus_tag="VFK23_06195"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET7318708.1"
                     /translation="MSHEQKIIPVALRDFALPVPRAGSIEAYSGHGRAAEEGREIHVR
                     VQKKRAKSDPSYESEVRVSRSFDREGYRFRIDGRMDGIFRREPPLIEEIKTGFNVREL
                     GRLLSENPLAHPYGLQLATYGYFHWLEHKVVPELFFHLVSTRGSDSLDLEQPFDLRAY
                     EKWLELRLDELVLEARKAEKRAARRRKIAAEFSFPFATPRPGQVELMHAVEQGMSEGR
                     PMLIQAPTGMGKTAGVLYPVLKDALSRGRMVVYVTPKNSQHSVAEDAVTRFQEGGAKL
                     KSLTITAKGKICFKNEPLCDPGFCEYAGDYYAKIANSGLLDVLAKKKKLKARTFRELG
                     KTYEVCPFELQIDAAPEADVIICDYNYVFAPRSSLGRMAAMDVDQEGKPNLVIDEAHN
                     LPSRAMDYYSPAVSTVTLEQMRGEVRALPARFRSESEELLDSCIRVIASCMPEESTRP
                     AKIDPPVDSFLEQDAKLRAFLSRYLDCDIEIERRDVILRLFFYWSAFAEALEYAADPS
                     RPEFFTTFQPHAGGGMVKITCCDASAMLRDCYNGYEHAVGFSATLKPFDFYAKLSGLD
                     PGEVKTAEFPSPFPKEHRKILVIPQISTKYSDRDRNYAKIADAIQRIVSLRRGNYFAF
                     FPSFEFLERVLALFDPPAGFTIIRQDRDMKTSRVEAVLEHLREGSSPAILFAVQGGTF
                     SEGVDYPGEMAIGAFVVGPPLPNFDFEREQMREYYQKQYGAGFDYAYAIPAMSRAIQA
                     AGRVIRSETDRGLIVLMDNRFVQPIYSRSMPADWFESDVRELVSESILKDVAKFWGKS
                     PV"
     gene            2866..3114
                     /locus_tag="VFK23_06200"
     CDS             2866..3114
                     /locus_tag="VFK23_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008315407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HET7318709.1"
                     /translation="MKTAISVPDDLFEEAEKIAKEQHFSRSEVFVAALREYLEKRKSA
                     KLLAALNDAYAVTESPEEYAVRKKSRKRYAKAIRKERW"
     gene            3111..3479
                     /locus_tag="VFK23_06205"
     CDS             3111..3479
                     /locus_tag="VFK23_06205"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016952672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HET7318710.1"
                     /translation="MNIKQGDVFWLDLADPDGSEPGYRHPHVVVQNNVYNQSRINTVV
                     LCALTSNLKRASVPGNVLLKKGEANLKKDSVVNVSQIVTVDKSDLLERIGSLSHARMT
                     QIVAGVKLLVEPRDVPIIKE"
BASE COUNT          787 a         1106 c         1039 g          686 t
ORIGIN      
        1 gatagcggaa aagctcgtgg gcaaagagaa ggcgcagaag atcaaggaag caatgctgat
       61 tgattatgtg tgacaccatc atgaagttca cgatcctttg tttttttgca catccctttc
      121 gcgtcctgaa tgaaattcca gacccgtcta aggccacgtc cgctcgcccg cgcaaactcg
      181 gccttcgggc tcagacgccc tgcactcgtg ctagcgcact cgtaaccgct gggtgcaggc
      241 agtgcaacgc gttcgcctgt cgggacgctc ccttaggcca agacgggtgc cccaggaatt
      301 tcatagggcc gctccaggga cgtgcggggt tggggtagaa acaaggcttt aggcacgcaa
      361 gaaaaaggta acaagaatat tcaaagaaag atgcagtaac tctttgtgat tcatgtctca
      421 tgagcaaaag atcatccccg tagcgctccg cgattttgcc cttccggtcc cccgggccgg
      481 gagcatcgag gcctattctg gccacggccg cgcggccgaa gagggccggg aaatccacgt
      541 ccgtgtgcag aagaagcggg cgaagtcgga tccttcctac gaatcggagg tccgcgtcag
      601 ccgctccttc gacagggaag ggtaccggtt ccggatcgac ggacggatgg acgggatctt
      661 caggcgcgag ccgcccttga tcgaggagat aaagaccggc ttcaacgtcc gggaactcgg
      721 ccgcctgctt tccgaaaacc ccctggccca tccctacggc ctccagctcg cgacctatgg
      781 ctatttccac tggctcgagc acaaggtcgt ccccgagctt ttttttcacc tcgtctcaac
      841 ccgcggcagc gattccctgg acctcgaaca gcccttcgat ctccgcgcat acgagaaatg
      901 gctggagctc cggcttgacg aactcgtcct cgaggcacgg aaggcggaga agcgcgccgc
      961 tcgccgcagg aagatcgccg ctgaattctc cttccccttc gcaacccccc gtcccggcca
     1021 ggtagagctc atgcatgcag tcgaacaggg catgagcgaa ggccggccga tgctcatcca
     1081 ggccccgacc ggcatgggga agaccgcggg ggtcctctat cccgttctga aggacgccct
     1141 cagcaggggc cggatggtcg tctacgtcac gccgaagaac agccagcact ccgttgccga
     1201 ggatgcggtt acgcggttcc aggagggtgg ggcaaagctc aagtccctca cgattacggc
     1261 aaagggcaag atctgcttca agaacgagcc gctctgcgac cccggctttt gcgaatacgc
     1321 cggcgactat tacgcaaaaa tagcgaatag cgggctcctc gatgtcctcg cgaaaaagaa
     1381 gaagctcaag gcccgcacct tccgcgaact gggaaaaaca tacgaggtct gccccttcga
     1441 actgcagata gacgcggcgc cggaggccga cgtcatcatc tgcgactaca attacgtctt
     1501 cgcgccgcgg tcttccctcg gcaggatggc ggcgatggat gtcgatcagg aggggaagcc
     1561 caacctcgtt atcgacgagg cccataatct tccctcccgc gccatggact actactctcc
     1621 cgccgtctcc acggtcacgc tcgagcagat gcgcggcgag gtccgcgcac tccccgcgcg
     1681 gttccggagc gagtccgagg aactgctcga cagctgtatc cgggtgatcg cctcctgcat
     1741 gccggaggaa agcacaaggc cggcgaagat cgacccgccc gtcgactcgt tcctggagca
     1801 ggacgcgaag ctccgcgcct tcctctcccg ctacctcgac tgcgacattg agatcgagcg
     1861 tcgggatgtc atcctgcggc tcttctttta ctggtccgcg ttcgcggagg cgctcgaata
     1921 tgcagccgat ccctcaaggc cggaattctt tacgaccttc caaccccatg cgggcggcgg
     1981 catggtgaag atcacctgct gcgacgcgtc cgcaatgctc agggattgct acaacggtta
     2041 cgagcacgct gtcgggttct cggcgacatt aaagcccttt gacttttacg cgaagctctc
     2101 cggactcgat cccggcgagg tcaagacagc cgaatttccg agccccttcc cgaaagagca
     2161 ccgaaaaatc ctcgtcatcc cccagatatc gaccaagtat tccgaccggg accgtaacta
     2221 tgccaagatc gcagatgcca tccaaaggat agtatcgctg cgccggggaa actatttcgc
     2281 cttcttcccg agcttcgagt tcctcgaacg agttctcgcc ctgtttgatc cgccggcggg
     2341 cttcaccatc atcaggcagg accgggacat gaagacgtcc cgggtcgagg cggtcctgga
     2401 gcaccttcgc gaaggctcct ctcccgccat cctcttcgcc gtacagggcg gaactttttc
     2461 cgagggagtg gactatcccg gcgagatggc gatcggggcc ttcgtggtag gaccgccgct
     2521 tcctaacttc gattttgagc gcgagcagat gcgggagtat taccagaagc aatacggagc
     2581 cggcttcgac tacgcctatg ccatccctgc gatgtccagg gctatccagg cggcgggaag
     2641 ggtgatccgc tccgagaccg accgcggcct catcgtcctg atggacaacc ggttcgttca
     2701 acccatttat tcccgctcca tgcccgcgga ctggttcgag tccgatgtcc gcgaactggt
     2761 ttcggagagc atcctgaagg atgtggcgaa attttgggga aaaagcccgg tttagggatc
     2821 aatccgggta ttgacatcgg tataaccaat gggtatacta atcgcatgaa aaccgccata
     2881 tccgtccccg acgacctctt cgaggaagcc gagaagatcg cgaaggaaca gcacttttcc
     2941 cggagcgagg tgttcgtcgc cgccctcagg gaatacctgg aaaagcggaa gtccgcaaag
     3001 ctgctcgccg cgcttaatga cgcctatgcc gtgaccgaat ctccggaaga gtacgcggtc
     3061 aggaagaaga gcaggaagcg ctacgcgaag gccatccgga aggaacgctg gtgaacatca
     3121 aacagggtga tgtgttctgg ctggacttgg cggacccgga tggctccgag cccggatacc
     3181 ggcatccgca cgtcgtcgtc cagaacaatg tgtacaacca gagcaggatc aataccgtcg
     3241 tcctgtgtgc cctgacttcc aacctgaagc gggcatctgt ccccggcaat gtactcctga
     3301 aaaaaggcga ggccaacctg aaaaaggaca gcgtcgtcaa cgtctcccag atcgtgaccg
     3361 tcgataaatc cgatctcctt gaacgaatcg gctctctatc ccatgcccga atgacgcaga
     3421 ttgtggcggg cgtcaaactc ctcgtcgaac cacgggatgt acccataatt aaagagtagc
     3481 aaggttgtga gcggcaagaa agagtaatca tgaaagccgt cgtgatccat gcattcggcg
     3541 ggaatgaagt tgtcgaggtc agggacatgc cgatgccctc cgtagagccc gacgacgttc
     3601 tcatccgggt ccgcgcgg
//
LOCUS       DASSLD010000345         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_718936,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000345 DASSLD010000000
VERSION     DASSLD010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718936"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1289
                     /locus_tag="VFK23_06210"
     CDS             156..1289
                     /locus_tag="VFK23_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7318711.1"
                     /translation="MNEAGVISAEELKKRLDTNQVDFLFDLRNADEFKAWHIEGRKDF
                     PMLNIPQEEFVGEEEGHLGKFPRDKTIVTVCAHGDSSKYSADLLKDRGLDAVSLLGGM
                     DAWSGFYETHKVAENPAIYQIFRVARGCISYLIVSQGSAVAIDAARHTDRVLDMAKSF
                     GARIRHVLDTHLHADHISGGRELATRSGASYHLHPADAEEASYSFVPLADNQLFTFGT
                     SMLEVIHSPGHTPGSTSFLLDKKWLFTGDTIMKTSMGRPDLGGKADAWSLLLYETLFR
                     RYQDFGDGVIILPSHAASVREQDAAGVIATTMGTARKEGDLFQIRDKEAFAAFIRANL
                     LENPERYQDIRKVNLGMLRPDEAKQKELEIGKNLCGMAKKNPS"
     gene            1568..1885
                     /locus_tag="VFK23_06215"
     CDS             1568..1885
                     /locus_tag="VFK23_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318712.1"
                     /translation="MASVLNVKESKGFCDEVYTQLADMKEKIMKLKDRSAAARPGKEF
                     EGGMFGRHLGELADQIEWKLQILSHSCSIDWKGSADYAEEAQVRPAEKFGDVEFSPGY
                     VGG"
     gene            complement(1882..>2110)
                     /locus_tag="VFK23_06220"
     CDS             complement(1882..>2110)
                     /locus_tag="VFK23_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318713.1"
                     /translation="CKTCRFSEVALPAKKAGSKEPAFLRIPFFSDNPEESRETLPDYK
                     EIRHTWSPFTGFSPPLELGTVCVNVPCFFPA"
BASE COUNT          513 a          547 c          629 g          421 t
ORIGIN      
        1 gaattgtcac cctgagcgaa gcgaagggtc tgggatctat ttgaaaaacg agattcttcg
       61 cttcgcttta gaatgacagg cgaagggctc gcaatgacgg cttgacggag tagatgtaat
      121 aaaatttttc aaaagcacga aaggaggcgg atgccatgaa cgaggcaggt gtaatctccg
      181 cagaagaact gaaaaaaaga ctggatacga accaggtcga cttcctgttc gacctgcgca
      241 acgccgatga gttcaaggcc tggcatatcg agggcaggaa ggactttccg atgctgaaca
      301 tcccccagga ggagttcgtg ggggaagagg agggccatct cgggaaattc ccgcgggaca
      361 aaaccatcgt caccgtctgc gcccacggcg attcctcgaa atactcggcc gatctgctca
      421 aggaccgggg cttggacgcc gtcagcctcc tgggaggcat ggacgcctgg agcgggtttt
      481 acgagacgca caaggtcgcc gaaaatcccg cgatctacca gatctttcgc gtggcgcgcg
      541 ggtgcatttc ctacctcatc gtatcgcagg ggtccgcggt ggcgatcgac gccgcacggc
      601 acacagaccg cgtcctcgac atggcaaaat cgttcggcgc acgcatccgg cacgttctgg
      661 acacgcacct ccatgccgat catatctccg gaggccggga actcgcgact cgctccggag
      721 cgtcgtatca cctgcatccg gcggatgccg aagaggcaag ctactccttc gttccgctcg
      781 cagacaacca gctcttcacc ttcgggacct cgatgctcga ggtcatccac tcccccggcc
      841 acacgccggg cagcacgtca ttcctgctcg ataagaagtg gctgttcacc ggggacacaa
      901 tcatgaaaac gagcatgggc aggcccgact tgggcggcaa ggccgatgcc tggtctttgc
      961 tgctctacga gacgctcttc cgccgctacc aggacttcgg cgacggcgtc attatcctgc
     1021 cgagtcatgc ggcttccgtc cgggaacagg acgcggcagg cgtgatcgcg acgaccatgg
     1081 ggacggcgcg gaaagaaggg gacctgttcc agatcaggga caaggaggcc ttcgcggcct
     1141 ttattcgggc gaacctgctc gaaaacccgg agcggtatca ggacatccgc aaagtgaacc
     1201 tcggcatgct gcggccggac gaggccaaac agaaggaact ggagatcggg aagaacctct
     1261 gcgggatggc gaagaagaac ccgtcgtaat cgatggaact aaaatatcag cggggatcgt
     1321 catgaggttc acggttcttt ttttgcatat cctcttcgtg acttgaatga aatcttggac
     1381 tcgcctaagg ccacgtccgc tcagtcccgg gcattatttc gaaagccgct tcccctctcc
     1441 gttatgatac ggcgcacaag gaggctttgt gtgcgaactg ccggtgtaaa cagcaccatt
     1501 atgctcctct ttatttgcta taattataat aaataagaaa agaaggctgt ttggaaggag
     1561 gctgattatg gcaagcgttc tgaatgtaaa agagagcaag ggattttgcg atgaagtcta
     1621 tacccagctg gcagacatga aggaaaagat catgaaactc aaggatcggt cagccgcagc
     1681 acgtccggga aaagaatttg aaggcgggat gttcggacgg cacctgggcg agctcgcgga
     1741 ccagatcgaa tggaaactcc agatattgtc gcattcctgc tccattgact ggaaaggctc
     1801 cgcggattac gcagaggagg cgcaagtgag gccggcagaa aaatttggag acgttgaatt
     1861 ttctcccggg tatgtcggcg gttaagcggg gaaaaagcaa ggaacattga cgcatacggt
     1921 gcctagctca aggggaggag agaatcccgt aaagggagac catgtgtgcc gaatttcctt
     1981 gtagtcgggt agtgtttccc gtgattcttc ggggttgtcc gagaaaaacg ggatgcgcaa
     2041 aaaagcaggc tctttggaac ctgctttttt tgcagggaga gcgacttcgc tgaagcggca
     2101 ggtcttgcaa
//
LOCUS       DASSLD010000346         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1307157, whole genome shotgun sequence.
ACCESSION   DASSLD010000346 DASSLD010000000
VERSION     DASSLD010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307157"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2086)
                     /locus_tag="VFK23_06225"
     CDS             complement(<1..>2086)
                     /locus_tag="VFK23_06225"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HET7318714.1"
                     /translation="LMIPQEFRTEDLFSLVTPENIEALLRRKLEQTGFFGARFRENAE
                     RALLLPKSGFKRRMPLWLIRLRSKELLAAVKSYEDFPILLETWRTCLEDEFDLAHAKT
                     VLDELKEGEISVSGTVTRTASPFAGNLVWKQTNKYMYEDDTPILARESALRPDLIKEL
                     LFSSHLRPKIPADVVRVLDGKLKRTTAGYTPGSALELLDWVKERLLMPEPEWKELAAA
                     IRRDLDQDAEDWLLSESRKLFWLSWPGTRHPLLSTLEALPKALVAFRLHPDVMTLQPV
                     VSGEKAEALRRRVLSFLRDVPEAARAPEEYDLTNFLSEWLSYYGPVEKESLRHLLGIT
                     PERLDEAIEPLIESQDLVMDRFGEESTALEICDSDNLGMLLRMARRYRQPSFQALPLE
                     QLALFLAAFQGVAPRGEALEDLQERLEQLFGWSAPAPAWEEYLLPSRMQPYRSEWLDS
                     LTDSSDLIWFGCGQRRISLAFKDDLDLFRSHGREEAGDGELDHLIPDRRGRYSFFEIT
                     EFSHLDSVHATELLWKEAWKGRVTSDSFRTVRKGAMTGFTARAFTGEARLPSRRSGFN
                     RWKVTRPMEGHWLRIDAPPAERDLVQEEELVKDRIRLLFRRYGVLFRELLANELPLLQ
                     WREIFRSLRLMELSGEVLSGYFFEGISGPQFVSPEAFRMLQEPLPQDAVYWINATDPA
                     SLCGLGLDQVQGL"
BASE COUNT          408 a          639 c          651 g          388 t
ORIGIN      
        1 ggaggccttg cacctgatcc agtccgagac cgcacagaga cgcgggatcg gttgcgttga
       61 tccagtatac ggcatcctgc ggcaagggct cctggagcat ccggaaggcc tcgggagaga
      121 cgaactgcgg gccggaaatc ccttcgaaga aataccccga caggacctcg ccggaaagct
      181 ccatcaggcg aagcgaccgg aagatctccc gccactggag aagaggcagt tcgtttgcga
      241 ggagttcgcg gaaaaggacc ccgtagcgcc tgaagagcag ccggatgcgg tccttgacca
      301 gttcctcctc ctgcaccagg tcccgctccg caggcggcgc gtcgatcctg agccagtgac
      361 cttccatggg acgggtgacc ttccaccggt taaaacccga ccggcgcgac ggtaaacggg
      421 cctcgcccgt aaacgcccgt gccgtgaaac cggtcatggc gcccttccgg accgtccgga
      481 acgaatcact cgtgacgcgg cccttccatg cctctttcca gagcagctcc gtggcgtgga
      541 cgctgtccag atgtgaaaat tcggttattt cgaaaaaact gtatctgccg cggcggtcgg
      601 ggatcaggtg atcgagctcg ccgtcgccgg cctcctcccg gccgtgagaa cggaaaagat
      661 cgaggtcatc tttgaaggcc aggcttatcc gcctctgccc gcatccgaac cagatgaggt
      721 cgctggaatc ggtcaggctg tccagccact cgctccggta aggctgcatc cggctgggaa
      781 gaaggtattc ctcccaggcc ggggcaggcg cggaccaccc gaagagctgc tccagccgtt
      841 cctgtaaatc ttccagcgct tctccccgtg gagcgacacc ttgaaaggcg gccaggaaca
      901 gggcgagttg ttcgagggga agcgcctgga aggagggctg gcgataacgc cgcgccatgc
      961 gcagaagcat gccgagatta tcgctgtcgc agatttcgag agccgtgctt tcttccccaa
     1021 accggtccat gaccaggtcc tgggactcga tcaggggctc gatcgcctca tcgagacgct
     1081 cgggcgttat gccgagaaga tgccggaggc tttccttctc cacggggccg tagtatgaaa
     1141 gccactcgga caggaagttc gtgagatcgt attcctccgg cgcccgggcg gcctcgggga
     1201 catcgcgaag gaacgacagg actcttcttc tcagggcttc tgctttctcc ccggaaacaa
     1261 cgggctggag ggtcataaca tcgggatgaa ggcgaaaggc aaccagcgcc ttcggcagcg
     1321 cttccagggt ggacaggagc ggatgcctcg tgcccggcca cgagagccag aacagtttcc
     1381 tcgactccga taggagccag tcctccgcgt cctgatcgag atcccggcgg atggccgcgg
     1441 caagctcctt ccattcaggc tccggcatga gaagccgttc cttcacccag tccaggagtt
     1501 cgagggccga acccggcgtg tatcctgccg tagtccgttt cagtttcccg tccaggacgc
     1561 ggaccacgtc cgcgggtatc ttcggacgca ggtgagaaga gaacagcagt tccttgatca
     1621 gatcagggcg cagggcggac tccctggcga ggatcggcgt atcgtcctca tacatgtact
     1681 tgttcgtctg tttccagacg aggttcccgg caaagggcga ggcggtcctg gtcacggtgc
     1741 cgcttacgct aatctctcct tccttgagtt catcgagcac ggtcttcgca tgagcgaggt
     1801 cgaattcgtc ctcgaggcag gtgcgccagg tttcaaggag gatcgggaag tcctcatagg
     1861 atttcacggc tgcgagcagc tctttcgacc tgagccggat gagccagagt ggcatcctcc
     1921 gcttgaagcc ggatttgggg agcaggaggg cccgctccgc gttctcccgg aaccgggccc
     1981 cgaaaaatcc ggtctgctcg agcttcctgc gcagcaaggc ctcgatgttc tccggcgtga
     2041 cgagggaaaa gagatcctcg gtcctgaact cctgcggtat catgag
//
LOCUS       DASSLD010000347         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1533276, whole genome shotgun sequence.
ACCESSION   DASSLD010000347 DASSLD010000000
VERSION     DASSLD010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533276"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFK23_06230"
     CDS             complement(<1..317)
                     /locus_tag="VFK23_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010480307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7318715.1"
                     /translation="MPGIQTFDDHVDEYEQWFVDHPLAYVSELHAVRDLLPKSGSGVE
                     IGIGTGRFAAPLGIKRGIEPSKPMAELARRKGVEVTSGVAEKLPFKDGEFDFALMVTT
                     VCFL"
     gene            complement(323..973)
                     /locus_tag="VFK23_06235"
     CDS             complement(323..973)
                     /locus_tag="VFK23_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET7318716.1"
                     /translation="MSGKKTFSVRPFDKLRKTMEQHAASAAPGPPRQKKKEEYTDKEL
                     FENAMDGVQEIEIFRELSCAHRPRSTAQTAHKADPEKKALEALAEIAAGARPIHLPDT
                     QEYVEWTNPGYHEHIIPKLHGGHFSVQAFLDLHGCTVPEAEEELETFFQESFRQGFRC
                     VKIIHGRGLRSVRGPRIKDAVIRRLSGHFRKDIIAFVTARQCDGGLGALYVLLRKK"
     gene            complement(1128..1535)
                     /locus_tag="VFK23_06240"
     CDS             complement(1128..1535)
                     /locus_tag="VFK23_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318717.1"
                     /translation="MIKSRFAVFMLLAVFIAFAAHAGEFAALKLKMLEARYSLYILLD
                     NGDKRGPKQQKRVQETADAVSAMLAGMKAPAGREAQFKELDSTWKAFKKTREEELVPL
                     ILARKQKEAEEVATGVQNDRFKKMMDLCDALEK"
     gene            complement(1819..>2473)
                     /locus_tag="VFK23_06245"
     CDS             complement(1819..>2473)
                     /locus_tag="VFK23_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7318718.1"
                     /translation="VAFWVMEYLGHDKVQVLNGGIDAWLKAGKKLTNEPSIKPEKTFT
                     AKVVPARYASTDEMLQIAQGKKSGVTVIDARSKKEHEGEDIRSLRGGYVPNTTVNIPH
                     TDTMDQEKDAKTGKDKDNGYITPDRVAGFYKDLDKNKRTIAYCHTGTRSTLSYLEMRL
                     LGFKEPANWDDSWIYWGNNLRYPVANEQWINFERLKKVEEETTKIKEAMPKKEEAQQ"
BASE COUNT          430 a          725 c          691 g          627 t
ORIGIN      
        1 agaaagcaga ccgtggtcac catgagcgca aaatcgaact ccccgtcctt gaacggaagc
       61 ttctccgcga cgcccgatgt gacctcgact cccttccttc gcgcgagttc cgccatcggc
      121 tttgacggct ctatccctcg tttgatcccg aggggcgccg caaacctacc ggtcccgatg
      181 ccgatctcga ctccgcttcc gcttttcggc aggaggtcgc ggaccgcgtg aagctcggac
      241 acgtacgcga gcggatggtc gacgaaccac tgctcgtact cgtccacatg gtcgtcaaag
      301 gtttgtatgc ccggcatatg ggttattttt ttctcaacaa gacatacagc gcccccaggc
      361 caccgtcgca ctgacgcgca gtcacgaagg cgatgatgtc cttgcggaag tggccggaga
      421 gacgcctgat caccgcgtcc ttgatccgcg gccccctgac ggaacggagt cccctgccgt
      481 gaatgatctt gacgcagcga aaaccctgcc tgaaggactc ctgaaaaaaa gtttcgagtt
      541 cctcctccgc ctccggaacg gtgcagccgt ggaggtcgag gaatgcctgc acggagaagt
      601 gccctccgtg cagtttcggg ataatgtgtt catgataccc ggggttcgtc cattcgacgt
      661 actcctgcgt atcgggcaga tgaatcggcc gcgctcccgc ggcaatttcg gcgagcgctt
      721 cgagcgcttt cttttcagga tcggccttgt gcgccgtctg cgccgtgctc cgggggcggt
      781 gtgcgcatga cagttcccgg aatatctcga tctcctggac gccgtccatc gcgttttcaa
      841 acagttcttt gtcggtgtat tcttcttttt tcttttggcg gggagggccg ggtgcggctg
      901 aggcggcgtg ttgctccatc gtcttcctga gcttgtcaaa gggccgtacg gaaaatgtct
      961 tcttaccgct catggcacat acccgcgtgc tgcagagaac ctgtctttgc cgcgggtatt
     1021 ataccagatt gccggaccgg atacgatact tatccggcat gttgactctc aaacaggagc
     1081 atgtcgtctt gcagagtaat gaatgcctgg acctggttat gtctcgccta cttttctaag
     1141 gcatcgcaga ggtccatcat tttcttgaat ctgtcgttct gaacgccggt ggcgacctct
     1201 tccgcctcct tctgctttct ggccaggatc agggggacca attcctcctc ccgtgtcttt
     1261 ttgaatgcct tccaggtgct gtccagttcc ttgaactggg cctctcttcc ggcaggtgct
     1321 ttcatgccgg ccagcatggc gcttaccgcg tcagcggtct cttgcacccg tttctgctgt
     1381 ttcggccccc gcttgtcgcc gttgtccagc aggatataga gggaataccg tgcctcaagc
     1441 atcttcagtt tcaaggcggc gaactctccc gcgtgagcag caaaggcgat gaagacggcc
     1501 aacagcatga aaacagcgaa tcgcgacttt atcatgatta cactctcctc ttcgtgattt
     1561 tctccggctg gcaggatacg gcagtttgaa ggccgcagat taaagtatat cactcactgt
     1621 ccaggtttaa gcggggggag ggacgccggc gaaacgccat tggttgttca tgggacattt
     1681 cttaacagaa ttgttcgatt ttctacaaat cgaggccggc gataggacat aagtaagaga
     1741 gagccttcaa cccctgccgc ggagcaaaaa aaccgccgaa ttgtccttcg gcggttgaga
     1801 ggtgtgcgca ctgtctgctt attgctgggc ttcttccttc ttgggcattg cttctttgat
     1861 cttggtggtc tcttcttcca ccttcttcag ccgctcgaag ttgatccatt gttcgttggc
     1921 aacgggatag cggagattgt ttccccagta gatccaggag tcgtcccagt tcgccggttc
     1981 cttgaatccg aggagcctca tttccaggta ggaaagcgtc gaacgcgtcc cggtgtggca
     2041 gtaggcgatg gtgcgcttgt tcttgtccag gtctttgtag aagcccgcga cgcggtccgg
     2101 cgtgatgtag ccgttgtcct tgtccttgcc ggtcttggcg tctttctcct ggtccatggt
     2161 gtccgtgtgc gggatgttga cggtcgtgtt cggcacgtag cctccccgaa gggaccggat
     2221 gtcctcgccc tcgtgctctt tcttggaccg cgcgtcgatc accgtgacgc ccgacttctt
     2281 cccctgcgcg atctggagca tttcgtccgt cgacgcgtat cgggccggga cgacctttgc
     2341 cgtaaaggtc ttctccggct tgatcgacgg ctcgttggtc agttttttcc cggctttgag
     2401 ccaggcgtcg atgccgccgt tcagcacctg gaccttgtca tgacccaggt actccatgac
     2461 ccagaaggcc acg
//
LOCUS       DASSLD010000348         4989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_623139,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000348 DASSLD010000000
VERSION     DASSLD010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623139"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VFK23_06250"
     CDS             <1..841
                     /locus_tag="VFK23_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET7318719.1"
                     /translation="DEVVAIGAGIQGAVLTGQVKDVLLLDVTPLSLGIETLGGVTTRL
                     IERNTTIPTKKSQVFSTASDNQTAVSIHVLQGEREMAADNKTLGRFELVGIPPAPRGI
                     PQIEVSFDIDANGIVHVMAKDLGTGREQSIKITASSGMSKEEIDKAVKEAEAHAAEDK
                     KKKEVAEARNEADTLIYSVEKSVADYGSKLSESERADVQKALEQAKAVKDGQDVDAIK
                     KASAELAKASHKLAEEVYSKMNKEQGQPGAGPTAGAEGGDGAKSGEKVVDAEFEEVDK
                     DKK"
     gene            1056..2177
                     /gene="dnaJ"
                     /locus_tag="VFK23_06255"
     CDS             1056..2177
                     /gene="dnaJ"
                     /locus_tag="VFK23_06255"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012312640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET7318720.1"
                     /translation="MAKDYYTLLGVHRNATEAELKRAYRKLAHEYHPDKNPGNKVSEE
                     KFKEINEAYEVLSDAQKRAYYDQFGVAPGAQAGGPGFSGFGAGAGMGDIFGDIFSEFF
                     GGGQGRSRATAGDDLRYNLKITFEEAAFGTTTKIRVPRWERCPDCDGTGAKTKDSVKA
                     CPNCRGTGQIRTQQGFFSISRTCSRCGGEGRIITDPCNTCGGRKRIERERTLSVKIPA
                     GVETGNTIRLTGEGEIGTYGGPPGDLYVFLMVEDHPLFKREGQDIICEVPISFVQAAL
                     GVEIDAPTLNGSVRLKIPAGTQPGQTIRLRGKGFPHLRGGGAGDQVCRINVEVPTKLT
                     AKQKELLQEFERLNSEDSTPLMKGFFDKVKELFGDTTKK"
     gene            2331..2831
                     /locus_tag="VFK23_06260"
     CDS             2331..2831
                     /locus_tag="VFK23_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HET7318721.1"
                     /translation="MDALEIAMKMETDAISFYTEAARKTTYPAGKKMFQTITEDEKRH
                     LEMISQIIKGLKVTHKDVSPMKNVKTVFEAMKNEMMKKVEATADELEAFKIAMQMEKE
                     GKEFYEKTLARAKTDKEKTLLKQLINEEEQHYAIFANTYDFLANTGNWFMWEELSIVD
                     GGTPWA"
     gene            2951..3226
                     /locus_tag="VFK23_06265"
     CDS             2951..3226
                     /locus_tag="VFK23_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318722.1"
                     /translation="MTYSNVALKDKILEMYPEIREHNFSVGLDFDEGKNAYILTFKRG
                     AETLTTHLEKKDADDCMSGIKCVYLGFQVAQFIKNFEERETFGRKAA"
     gene            complement(3480..4580)
                     /locus_tag="VFK23_06270"
     CDS             complement(3480..4580)
                     /locus_tag="VFK23_06270"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7318723.1"
                     /translation="MKWLETPSIYQIAKDELYELDGDSFRFLKDCMTVDGCSSKDGAF
                     IDYCLDEGLLTTEKVTVARPALKQAPEPSLRYLELQITDTCNLRCKHCYLDNPSHNEL
                     SPEEVNRALKEFEAMQGLRVLITGGEPLLHGKFQEINEMLPDLFIRKVLFSNGLLLKK
                     DVLGTLKVDEIQISIDGLEQAHDHVRGQGTFKRAMEAAQDALDAGFDVSISTMVHRGN
                     LSDFDAMERLFKGMGVKDWTVDVPCVTGLLKENDELCVSPEEGGKYLGYGYGGGLHAS
                     GQGYGCGLHLMSVMADGKIAKCSFYRDSAVGVIAEGLGAAWGKIRPVRLSDLSCNCEY
                     LEICRGGCRFRAELLAGKSGKDLYRCALYGTI"
     gene            4660..>4989
                     /locus_tag="VFK23_06275"
     CDS             4660..>4989
                     /locus_tag="VFK23_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318724.1"
                     /translation="MVKNATLVKTLCLPYCRYYKPDRNEELLCRGAVVVQRLMESGRS
                     LSTRENPHQGIDCEHREVVTQQVCGSCEFREQDCDFAQDRAACPCGGFALLLGLLRAG
                     VITSGDLR"
BASE COUNT         1213 a         1368 c         1470 g          938 t
ORIGIN      
        1 cgacgaggtt gtggcgatcg gcgccggcat ccagggcgcg gtgcttaccg gccaggtaaa
       61 ggacgtcctg cttctggacg tgactcccct gtccctgggc atcgagaccc tggggggcgt
      121 gaccacgcgc ctcatcgagc ggaacaccac cataccgacc aagaagagcc aggtcttctc
      181 cacggcctcg gacaaccaga ccgcggtctc catccacgtg ctccagggcg agcgcgaaat
      241 ggccgcggac aacaagacct tgggccggtt cgagctcgtc ggcattccgc ccgcgccgcg
      301 cggcatcccg cagatcgagg tgagcttcga catcgacgcg aacggcatcg ttcacgtgat
      361 ggcaaaggac cttgggacgg gcagggagca atccatcaag atcacggcgt cgtccggcat
      421 gagcaaggaa gagatcgata aggccgtaaa agaggccgag gcgcacgcgg cagaggacaa
      481 gaagaaaaag gaagttgccg aggcgcggaa cgaagcggac acactcatct actccgtcga
      541 aaagtccgtt gccgactacg gctccaagct gtccgagtca gagcgcgccg atgttcagaa
      601 ggcgctcgaa caggcgaagg cggtgaagga cggccaggac gtggacgcca tcaagaaagc
      661 gtcagccgag cttgcgaagg cgtcccataa gctcgccgag gaggtctact ccaagatgaa
      721 caaggagcag ggccagcccg gggcgggccc caccgccggc gcggagggcg gagacggcgc
      781 caagtccggc gaaaaggtcg tggacgccga gttcgaggaa gtggacaagg acaagaagta
      841 gctgggtacc atggaagagg atagcaggtc gcgcggggca gtgaaggagc gcctgctatc
      901 ctttttttgt aatatccgtg atgtcatatc tgcgaaagca ggtatccatt gtcatcttct
      961 cagatgaaat tcaaaatgga tttccgcttc agcgtggccc gctttatcgg ccgtcgcggg
     1021 actgacaaaa tagcgctgta atcattaagg ggcttatggc caaagactat tacacactgc
     1081 tcggcgtaca ccggaacgcg accgaggccg aactcaagcg cgcataccgg aagcttgcgc
     1141 acgaatatca tcccgataag aaccccggga acaaggtctc cgaggagaag ttcaaggaga
     1201 tcaacgaagc ctatgaagtc ctctccgacg cgcagaagag ggcgtattac gatcagttcg
     1261 gcgtcgctcc cggcgcacag gccggcggac cgggattcag cggattcggg gctggggcgg
     1321 gcatgggcga catcttcgga gacatcttca gcgagttctt cggagggggg caggggcggt
     1381 cccgcgccac ggccggcgac gacctcaggt acaatctgaa gatcacgttt gaggaagcgg
     1441 cgttcgggac gacgacgaag atccgcgttc cccgctggga gcggtgtcct gactgcgacg
     1501 gcaccggcgc caagacaaag gactctgtaa aggcttgccc gaactgccgc ggaacggggc
     1561 aaatcaggac ccagcagggt tttttcagca tcagccgcac ctgctcgcgc tgcgggggag
     1621 agggcaggat catcactgat ccctgcaaca cctgcggcgg acgaaaacga atcgagcggg
     1681 aacggacgct gtcggtgaag atcccggcgg gagtggaaac gggcaacacc atacgcctca
     1741 ccggcgaggg agagatcggc acctatggag gcccccccgg cgacctgtac gtgttcctca
     1801 tggtcgagga ccatccgctc ttcaagcgcg aaggccagga catcatttgc gaagtgccga
     1861 tcagttttgt ccaggcggcg ctgggagtgg aaatcgatgc tccaacgctc aacggcagcg
     1921 tgaggctgaa gatccccgcc ggcacccagc cgggccagac gatcagactt cgggggaagg
     1981 gttttcccca ccttcgcggc ggcggggctg gagaccaggt ctgcaggatc aacgttgaag
     2041 tcccgaccaa actgacagcg aagcagaagg aactgcttca ggagttcgaa cgcctgaact
     2101 cggaggattc aacacccttg atgaaggggt ttttcgacaa agtcaaggaa ctgttcggag
     2161 acacgacgaa aaagtaattg aacgcactcc tgccgcttta ttgtacgccg tgactggtaa
     2221 tcatcccgcc gcgtagtaaa atcctccgcc ggccgtctgt cctttctcct gccccagtgt
     2281 gatataataa ttttaagata tttccccgaa aaaatctgga ggaggtcttc atggatgccc
     2341 tggaaattgc gatgaaaatg gaaacagacg ccatatcttt ctacaccgag gcggcgcgga
     2401 aaacgacata ccctgccggg aaaaagatgt ttcagacaat cacggaggac gaaaaacggc
     2461 atcttgaaat gatctcccag atcatcaaag gcctcaaggt aacacacaag gacgtcagtc
     2521 ccatgaaaaa cgtcaagacg gtgtttgaag ccatgaaaaa cgagatgatg aaaaaagttg
     2581 aggccacggc agacgaactt gaggctttca agatagccat gcaaatggag aaggaaggga
     2641 aggagttcta cgaaaagaca ctggcccgcg ccaagaccga taaggaaaag acgctgctga
     2701 agcagctcat caacgaggag gagcagcatt atgcaatctt tgcaaacacc tacgacttcc
     2761 tcgcgaatac gggaaattgg ttcatgtggg aggagttaag catcgttgac ggcggtaccc
     2821 cgtgggccta gaaaattcgg aattcggggt gcggaattcg gaatgaaagg cgaaaagaac
     2881 ggcaaaagat ttcttggttt ctattccgca atccgcattc gccattccgc atttgaaaaa
     2941 gggggttgcc atgacataca gcaatgtcgc attaaaggat aagattctgg aaatgtatcc
     3001 cgaaatcagg gagcataatt tctcggtggg actcgatttc gatgaaggaa agaacgccta
     3061 tatcctgacg ttcaaacgcg gcgctgaaac gcttacgacg catctcgaga aaaaggacgc
     3121 cgacgactgc atgagcggga tcaagtgcgt atacctgggc ttccaggtcg cccagttcat
     3181 caaaaacttc gaggaacggg aaacgttcgg ccggaaagcc gcttagaaga cgatccggac
     3241 agggaacaga tgctccgcaa ataaaaagaa gcccggtgct ttcgcaccgg gctctttatt
     3301 tctcctgggg cataggatgc tgatgatcag caggaacttt tgcacttgca ggtaacagcc
     3361 atttttttcg tcactttctt gatcgtcatc gcactgcctc ctttccgaaa tgatcacgcc
     3421 cgaagaacgg ttcgccggga gagctcagac ccgagcccgg atgaagttga aaaatcactt
     3481 tatattgtac cataaagtgc gcagcggtaa aggtccttgc cgctttttcc tgcgaggagt
     3541 tcggcccgga accggcagcc tccgcggcag atctcgagat actcgcagtt gcaggaaaga
     3601 tcgctcagcc gcacgggccg gattttcccc cacgccgcac cgagcccttc cgcgatgacg
     3661 ccgacggcgc tatcccggta aaacgagcat ttcgcaatct tcccgtcggc catcaccgac
     3721 atgaggtgca gtccgcagcc gtatccttgg ccggatgcat ggaggcctcc cccataacca
     3781 tagcccaggt atttaccgcc ttcttcggga ctgacgcaca actcatcgtt ttccttgaga
     3841 aggccggtaa cgcaaggcac atccacggtc cagtccttga ctcccatacc cttgaagagc
     3901 cgctccatgg cgtcgaaatc ggaaaggttc ccgcggtgca ccatggtcga gatcgagaca
     3961 tcgaaacccg catcaagcgc gtcttgcgcc gcttccatgg cgcgcttgaa cgtcccctgt
     4021 ccgcggacat ggtcgtgggc ctgctccagc ccgtcgatac tgatctggat ctcatcgact
     4081 ttcagggttc cgagcacgtc cttcttcaga agaaggccgt tcgagaacag gaccttcctg
     4141 ataaataaat cggggagcat ctcatttatt tcctgaaact tcccgtgcag gagcggctcc
     4201 ccgccggtta tgagcacccg caggccctgc atagcctcga attccttcag agcgcggttc
     4261 acctcttccg gcgaaagttc gttgtgcgac ggattgtcca gatagcaatg tttgcatcta
     4321 aggttgcagg tgtccgtgat ctgcagttca aggtaccgga gggagggttc aggggcctgc
     4381 ttcaaggcgg gtcgcgcgac cgtcaccttc tccgtggtaa gcaacccctc atccagacag
     4441 taatcgatga acgcgccgtc cttggacgaa caaccgtcta ccgtcatgca atccttcaga
     4501 aaacggaagg agtcgccgtc gagttcgtag agttcgtctt ttgcgatctg ataaatagac
     4561 ggagtttcga gccatttcaa ggcggcccgg cgggacaaat aataactcat agcgataatt
     4621 atagcaccgt gatttctatt ttgttataat caggatgcga tggttaaaaa tgcgaccctt
     4681 gtaaaaacgc tgtgtctgcc gtactgtcgc tactataagc cggacaggaa tgaagaactg
     4741 ctctgccggg gagcggttgt cgttcaacgg ctgatggagt cgggccggag cctgtcgacc
     4801 cgcgagaacc cgcatcaggg tatcgactgc gaacaccgcg aggtggtcac gcagcaggtc
     4861 tgcggctcct gcgaattccg ggagcaggac tgcgactttg cacaggaccg ggcagcatgt
     4921 ccgtgcggcg gatttgcgct cctcctgggg cttctgaggg ccggtgtgat cacgagcggg
     4981 gacctgcgg
//
LOCUS       DASSLD010000349         2566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_654320,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000349 DASSLD010000000
VERSION     DASSLD010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654320"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1897
                     /locus_tag="VFK23_06280"
     CDS             <1..1897
                     /locus_tag="VFK23_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HET7318725.1"
                     /translation="FALSQARFAVSFHPDKGKFIGREALQEQFDDLQKILKRDFGGVV
                     RLKQIIQPIALVGKGVARHGDKVFVSDQQVGWITSGTVAPYWKTTGEGLESRMIDETG
                     KRPVCLGLIDSTLQIGQELDIDVRGKRIKAVIVAHHLRSEAPPRARAIIREEDGSGAG
                     VQERQENFVTEEVRMRVNALLDKAIENTRWRQEQCINLIPSEMTASPMVRLLSIMDPS
                     FRYAEHKKVKALHDADVFYYQGTGFINEVERLIAEEMRTYLGCRNIESRVISGQMANA
                     AVFSALVDFVNRGDRKREPRRLRPVMNNHIIKGGHLSAQPMGALHDFIARDPLTERPA
                     IVNFPVLPENPYKIDIVETRRLLDEHRPELIILGKSMVLHKEPVAEIRACLDELGLRA
                     VLMYDMAHVLGLAGPHFQQPFVEGADIVTGSTHKTFFGTQRGIIANNFAEDDQQYPLW
                     EAIETRTFPGSVSNHHLGTLLGLLMAAYEMNHFKDEYQKSVVGNAKSFAKSLADAGLR
                     VAGDPAISYTETHQVILQVGYAKGIEAAKQLEENNIIVNFQATPDEEGFTASGGLRLG
                     VSEMTRFGFGNDEFRKLAGLIRDVVLDGKKVRDEVTILRKDYQDLKFCFSEEQFAGRI
                     EALHRLI"
     gene            complement(2052..2453)
                     /locus_tag="VFK23_06285"
     CDS             complement(2052..2453)
                     /locus_tag="VFK23_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET7318726.1"
                     /translation="MKYMLDTNICIYLIRQKPPKVLKHSKSHAVGDIGISSITLVELR
                     YGVSKSLHVEKNREALDEFILPLEIADFDEKAAREYGEVRAELERAGKPVGSMDMLIG
                     AHALAQGATLVTNNTREFRQIKNLKIVDWSA"
BASE COUNT          559 a          718 c          771 g          518 t
ORIGIN      
        1 tttcgccctt tcccaggccc gttttgccgt cagtttccat cctgacaagg ggaaattcat
       61 cggccgggaa gcccttcagg aacagtttga cgatcttcag aagatcctga aacgcgattt
      121 cgggggggtg gtcaggctga aacagatcat tcagcccatc gcactcgtcg ggaaaggggt
      181 cgcgcggcac ggggacaagg tctttgtgag cgatcaacag gtcggatgga tcaccagcgg
      241 aaccgtcgcc ccctactgga aaacaacggg agaggggctt gagagccgca tgattgacga
      301 gacggggaag aggccggtct gtctcggact gatcgattcc actcttcaga tcggccagga
      361 gcttgacatt gacgtgcgcg gcaagaggat caaggcggta atcgtagcgc accatctgcg
      421 gtccgaagcg cctccccggg cccgggcgat catccgcgaa gaagacggga gcggcgcagg
      481 ggtgcaggag aggcaggaaa atttcgttac cgaggaggtc cggatgcgag tcaacgcgct
      541 tcttgacaag gctatcgaga acacccgctg gaggcaggag cagtgtataa acctcattcc
      601 ctcggagatg acggcttccc ccatggtgcg gcttctgtcg atcatggacc cctcgttccg
      661 gtacgcggag cacaagaagg tcaaggcgct tcacgatgcc gatgtgtttt actatcaggg
      721 tacggggttc atcaatgaag tggagcggct gatcgccgag gagatgcgaa catacctcgg
      781 ctgccgaaat atcgaaagcc gcgtcatcag cggtcagatg gccaacgcgg ccgtgttcag
      841 cgcgctggtg gattttgtca accgggggga ccggaaacgg gagccgcggc ggcttcggcc
      901 cgtgatgaac aaccacatca tcaaaggcgg ccatttaagc gcccagccca tgggcgcgct
      961 gcacgatttc atcgcgaggg acccgctcac ggagcggccg gcaatcgtca actttccggt
     1021 gcttccggag aacccgtaca agatcgacat cgtggagacg agaaggctgc tcgatgaaca
     1081 ccggcccgag ttgatcatcc tcggaaagag catggtgctt cacaaggaac cggttgccga
     1141 gatcagagca tgcctggacg agctcggcct gcgcgcggtc ctgatgtacg acatggccca
     1201 tgtcctcggc ctggcagggc cccatttcca gcagcccttt gtagagggtg cggacatcgt
     1261 gaccgggtcc acccataaaa cattcttcgg cacgcagcgc gggatcatcg cgaacaactt
     1321 cgccgaagat gatcagcaat accccctctg ggaggcgatc gaaacaagga cgttccccgg
     1381 cagcgtgagc aaccatcatc tcgggaccct gctgggactc ctgatggccg cctatgagat
     1441 gaaccacttc aaggacgaat accagaagag cgtggtcgga aacgcgaaga gcttcgcgaa
     1501 atccctcgcc gacgccggcc tgcgcgtggc gggcgacccc gccatctcct acaccgaaac
     1561 ccaccaggtc atcctccagg tgggctatgc aaaggggatc gaggcggcga aacaactcga
     1621 ggaaaacaac atcatcgtca atttccaggc tacgcccgat gaggagggat tcaccgcgtc
     1681 aggagggctc cggctcggcg tttccgaaat gacgcggttc ggattcggga acgatgaatt
     1741 caggaagctc gcgggcctga tccgggatgt cgttctggac gggaagaagg tgcgggatga
     1801 ggtgaccatc ctgcgcaagg actaccaaga cctcaagttc tgcttttccg aggaacagtt
     1861 tgccggccgg atcgaggcgc tccatcgact tatctgatca cgggatgcga cgacttgcct
     1921 gaatgtttct acgagctacg agagtcaaac attaaagacc tgaccctaag atgtccctga
     1981 ccctaagatg tccctaagat gtccatgctg gggcaggctc cagcaggaat ccaatttcct
     2041 atagtgcggg gttatgcgct ccaatcgacg atcttcaggt tcttgatctg cctgaactct
     2101 ctggtgttat tggtaacgag tgtcgcgccc tgcgcgagcg cgtgcgctcc gatcagcata
     2161 tccatcgacc cgaccggctt tcctgcccgt tcgagttccg cccggacctc cccgtattcc
     2221 cgtgcggcct tttcgtcgaa gtccgctatt tcgaggggga gtatgaactc atcgagcgct
     2281 tcacggtttt tttcgacgtg caggcttttg gatacgccgt atcggagttc cacaagggta
     2341 atggaggaaa tgccgatgtc gccgacagca tgggatttgg aatgcttcag cactttggga
     2401 ggtttttgcc tgatgaggta aatgcagata ttcgtatcca gcatatactt catcagaagt
     2461 cctcccgctt ttgcggcttc ggctgccttc tttccgccat gaagtcatcg gagaatttgt
     2521 ccaggctgtg gaccagggtg tcccatgagt tctttgccgg aatcag
//
LOCUS       DASSLD010000350         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_41404,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000350 DASSLD010000000
VERSION     DASSLD010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41404"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(656..973)
                     /locus_tag="VFK23_06290"
     CDS             complement(656..973)
                     /locus_tag="VFK23_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET7318727.1"
                     /translation="MNTELLLADILEELYEAKKTGALYIRILETSEDLYRIFFKNGNI
                     CHIRYGSAVGKDCLDILEYYNLVGATFFDGINAPDRPASDLPDTEQIISLIRGFDKKV
                     SMR"
     gene            complement(1048..>2334)
                     /locus_tag="VFK23_06295"
     CDS             complement(1048..>2334)
                     /locus_tag="VFK23_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7318728.1"
                     /translation="AVGGYHNIQEVKIFSPNGVILKSSKSWMIGRNVDPFTQKWSLAG
                     KFKKPIKRREEGIFSVLVPIENDQRCFHCHGSKAKLNGVLAVDVSMAQTQEKVRELSK
                     TMFLWAFGVTAILAVSLSLFLTHLVSNPIQELIATMERAERGLEARVDVKSFDDIGRL
                     GEAFNSLLSKLERARRRVERYHYEQMKRAERLASIGEMAAGIAHDIKNPLAGIAGVIQ
                     IIKKEETMGDQKRVVLDEVLAQVQRMDKAVRNLLSFARPPEPKMTVSDINEIISKLLD
                     FLAPQFAKNHIMTERKLAPGLPWLTLDPDLLQQAFINIALNAIQAMPEGGKFVAETRT
                     TKPTVETPGSVEIVFSDTGKGVQPENLVKIFNPFFTTRQQGTGLGLAITQRIIEQHGG
                     EITVTSAPDKGATFTIVFPYPQRDASERKTEDSWEL"
BASE COUNT          488 a          652 c          659 g          535 t
ORIGIN      
        1 aaatattcca ccacagagga cgcagaggaa cgcggaggaa atctctgtct ccccgcctcg
       61 ctttccctct gcgtcctctg tggttaaatg gctctgctca aactaaattc aaaccaaaca
      121 atataatcca cccttgttcc ccggatgcct tattcactcg caagaaatat accggaatcc
      181 cgggaaaagc ctgagtggta ttcttgatgt aggaggaatg aaagtggccg tcactaatca
      241 attgaaatta tgatacttgc ttttgtcgca gagactttaa taaagttcag gggacgaaaa
      301 cggggagcac gaaaaaaagc gaaatttgct gcaggcgatc tcgcattacg cgagaaaaac
      361 gggaaatgtg tctccaaagg aaacaggcgg gaaatgaatg atggaaaatt attaggaggt
      421 tggtttcagt gtttcccaaa gatacaaacc gaggcgtgaa tgtgtttccc ggggaaacaa
      481 tcatatttta tagggggggg gacatcaagt tctgtcacat cagaggctgc ttcgaagcag
      541 ttagtcaaca gaaatttgca cgctcttcac tcgttcattg attgcttcac cgaccctgtc
      601 catccgatca tcaactactc atgcaccgat gaagtcccgt gcgatcccga caagtttacc
      661 gcatgcttac tttcttgtcg aaaccccgga tcaaggagat tatctgttct gtatccggga
      721 ggtcggaagc ggggcgatcc ggggcgttga taccgtcaaa gaacgttgct cccaccagat
      781 tgtagtactc gagaatgtcc aggcagtcct tgcccacggc ggagccgtaa cggatgtggc
      841 agatgttccc gttcttgaaa aaaatccggt acaggtcctc gctggtttcg agaatccgga
      901 tgtagagggc gcccgtcttt tttgcctcat aaagctcctc caggatatcc gcgagtaaga
      961 gctccgtatt catgttcttc tcctgtaaca ggatagcgcg ttccttgccg gccccggatg
     1021 gtgcgcagag cgcacgtcag gaagagctca caactcccag gagtcctccg tcttccgttc
     1081 cgacgcgtcc ctctgcggat aggggaagac gatagtgaag gtagcccctt tgtcaggggc
     1141 gctcgtcacg gtgatttctc ctccatgttg ttcgatgatc cgctgcgtga tcgcgaggcc
     1201 cagccccgtc ccctgctgcc tggtcgtgaa aaagggattg aatatcttga ccaggttctc
     1261 cggctggacg ccctttcccg tatcggagaa cacgatctcg acggaacccg gcgtttcgac
     1321 ggtcggtttc gtcgtcctcg tctcggcgac gaatttgccg ccctccggca tggcctggat
     1381 cgcgttcagg gcaatgttga tgaacgcctg ctggagcagg tccgggtcga gcgtcagcca
     1441 gggaagaccc ggcgccagct tccgttcggt catgatgtgg ttcttggcga actgcggcgc
     1501 cagaaagtcg aggagcttgc tgatgatctc gttgatgtcc gagaccgtca tcttgggctc
     1561 gggcggacgg gcgaacgaga ggaggttgcg gaccgccttg tccatgcgct gcacctgcgc
     1621 gagcacctcg tcaagcacca cccgcttctg gtcgcccatg gtttcttcct tcttgatgat
     1681 ctgaatgacc cccgcgatgc ccgcaagcgg gttcttgatg tcgtgggcga tgccggctgc
     1741 catctcgccg atggacgcga gccgttcggc cctcttcatc tgctcgtagt gataccgttc
     1801 caccctgcgc cgggcgcgtt ccagcttgga aagcagagag ttgaaggcct ccccgagcct
     1861 gccgatgtcg tcgaagctct tgacgtcgac gcgtgcctcg agcccccgct cggcgcgctc
     1921 catggtggca atcagctcct ggatggggtt cgagacgagg tgagtcagga agagtgagag
     1981 ggacaccgcc aggatggcgg ttaccccaaa cgcccagagg aacatggtct tgctgagttc
     2041 ccgcaccttc tcctgggtct gcgccatgga cacatcgacc gccagcacgc cgttcagctt
     2101 cgccttggag ccgtggcagt gaaaacagcg ctggtcgttt tcgatgggga ccagcaccga
     2161 gaagattcct tcctccctgc gcttgatcgg cttcttgaac ttcccggcga gggaccactt
     2221 ctgcgtaaag gggtccacgt tgcgcccgat catccagctc ttggaggact tcaggatgac
     2281 gccgttcggg ctgaagatct tcacctcctg gatgttgtgg tatccgccga cggc
//
LOCUS       DASSLD010000351         8733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1533450, whole genome shotgun sequence.
ACCESSION   DASSLD010000351 DASSLD010000000
VERSION     DASSLD010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 8733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8733
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533450"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1800)
                     /locus_tag="VFK23_06300"
     CDS             complement(130..1800)
                     /locus_tag="VFK23_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7318729.1"
                     /translation="MKIRDKLFLGFGLYIFLAVILGFFAYKELRTIGTRLTLVETADD
                     ITNTILEVRRYEKNFLLFKDRESYEELQKYLDTLKTNIDAIHAEIVTEIGSDNYTMMK
                     SAIAGYEGLIRNVSKKFSSEEEFEELVREEGRTVERRLSGASLRAFLIVRRYEKNVML
                     YRNRDAFAVFERALAAPALRSVPEIGNYQARVAGLYRLYEEEKEAIEKLRQTAREIQS
                     FTQNLSRRERSDIATMIRMSVNLLLLALVLIVVLGIIINIKLATSIATPIRTLEKITK
                     KISQGDFSEQIEVKGQDELSSLEASFNLMEEKLKNALWSLEHTIEKLREKQAQLVEAE
                     KLASVGKLAAGIAHEINNPLTAVLTFSNLMLEQCPSGDPRRERLKLMARETNRARNIV
                     RQLLNFGREIVIKPEMININRPVSEIADSLTAQDAFKGVELAMKLDENLPDVEADPAQ
                     VGQVVMNMLLNAIHAITPPGRIEVVTRKNGNAVEIVFSDTGRGIPEEHIHKIFDPFFT
                     TKEAFKGTGLGLAVSYGIIKKHGGDIEVASSEGKGTTFTVRLPIHGQV"
     gene            complement(1998..2735)
                     /locus_tag="VFK23_06305"
     CDS             complement(1998..2735)
                     /locus_tag="VFK23_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318730.1"
                     /translation="MIIKNAKTFNIGALFAITFMGVLIAIFSPLFQGKNGLEFADNSF
                     NKLAKGSSYFIPKVVKDNEQFMGKPFTVSIRTDKPEDKPGDAETRAANIAKVFTTAGA
                     KVDISGSTLKIEGDLGQVLASALQDADAMFKNDGEKIRARYGTDDEKKMFRQWHNALS
                     RMNKEFDKEKKITESKMVSAVVKKAVEPAYNFYQIDANRVSDHAGMLSGLLVFYVFYT
                     MWWGYAIFYMFEGFGLTMKKAKVKKEA"
     gene            complement(2737..4020)
                     /locus_tag="VFK23_06310"
     CDS             complement(2737..4020)
                     /locus_tag="VFK23_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7318731.1"
                     /translation="MNLARRVFEFLKMSSEAHARWDYEVSMSIIKNRKKWLILLAALL
                     PIALVMLAEASDMIGSKTAYAPAFYSTNIFLVSIAIGLCAGLITGCIGAGGGFIITPA
                     LMAAGVKGILAVGTDLFHIFAKAIMGTSVHKKLGNVSVKLAVAFLVGSAGGTFIGGAL
                     NKALYDKDPLLSEAFISLVYAVLLGFLGFYALADFLKSRKGKDTGDAHGGSSGMTELS
                     KKVQNINIPPAICFDEDYCKGGRKISGVIVALGGVVVGVMAAIMGVGGGFITFPMFVY
                     VFGVSSMTTVGTDILQIIFTAGLAAIGQYAIYGYVFYTLAMGMLLGSLIGIQVGALTT
                     KVVKGIHIRGFYAVSILAGFINRAATLPKKLVELEVVNMSPGVVKGIETFGNVIFWIV
                     VAAFAAWVISKFVMNINVLRGEDEKVEPVLVKEEV"
     gene            complement(4225..4389)
                     /locus_tag="VFK23_06315"
     CDS             complement(4225..4389)
                     /locus_tag="VFK23_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318732.1"
                     /translation="MNKLKKKIEDLMVAITFAEAGEYDEASRLSDDAKEEKILQTEGV
                     KKTAMERAGK"
     gene            complement(4572..5882)
                     /locus_tag="VFK23_06320"
     CDS             complement(4572..5882)
                     /locus_tag="VFK23_06320"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7318733.1"
                     /translation="MLNILIISDEAKDLYALFRSTGFSPDIQDPNRDAASHGSDAPDD
                     AAYDIVFIDLDTANWQERLLEIRHSTPVIAFSRSDLKKAVESLRLGASDYLEKPLTVE
                     SLGEVIARHKKKILSNKYGFDEIIGNSASMQEVFGLIKKAAASESNVLITGESGTGKE
                     LIARAIHRWSPRNDKYFMTINCSAIPDTLLESELFGFEKGAFTGAQYQKKGLLEQSDG
                     GTVFFDEIGDVSPLFQTKVLRVLQEGEIMRIGGSRQLKVDLRFIAATNRDLKASCQKA
                     LFREDLFYRLNVINIHLPPLRDRLEDVPLLAKHFVQAHAPKRKDILIKCISGEAMSIL
                     LEHSYPGNVRELENIIERAISFANTSEILPSDLPENLRLSPVRPAARASALRLKDTLS
                     KMEREAIVSALFEHNGNISRAAAALGIFRQQLQRKIKQYKIATQ"
     gene            complement(5939..6832)
                     /locus_tag="VFK23_06325"
     CDS             complement(5939..6832)
                     /locus_tag="VFK23_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7318734.1"
                     /translation="MAEICPTAGVERVLLSTDGSEFSEGAVREAIGLARKCGSHLTAL
                     SVIDTNPELDTLAPQIVEKKGKEARQNLDAVQARAKNEGVACSTVIREGEDAYQYIVD
                     EAVKDKSSVIVMGRRGRTGLKRLMMGSVTARVIGHAPCNVLVVPRAARLGFSNIVVAS
                     DGSKHSVAAASEAIGLAKRNGSMLTVVSVVPSEIATPVDIDFTVSQRELIAEKEMQEA
                     EKNARAVKDASQKEDVSVKALIMSGKPADAIIEIARERRAELIVLGSHGRTGIERLLM
                     GSVAERVIVLASCPVLVVKSR"
     gene            complement(7078..7347)
                     /locus_tag="VFK23_06330"
     CDS             complement(7078..7347)
                     /locus_tag="VFK23_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HET7318735.1"
                     /translation="MCRNIKPLFNYDPPVTDEEVRTAALQFVRKISGYNKPSKANEAA
                     FLEAVDGVSAVCRSLLNKLETDAPPRNRAEETARARARAARRFPS"
     gene            complement(7477..7974)
                     /locus_tag="VFK23_06335"
     CDS             complement(7477..7974)
                     /locus_tag="VFK23_06335"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HET7318736.1"
                     /translation="MIIIVMGVSGSGKTTVGRLLADELGWPFYEGDDYHPPANIQKMT
                     HGSALTDEDRSGWLDMLSQLIRELDGEGRSAVVACSALKQAYRDVLVGRRSTDVRFVY
                     LKGSRDLIRQRMSERRGHYMKAGLLESQFDILEEPAQSVVVDITESPGEIVNRVKRAL
                     GLTNV"
     gene            8238..>8733
                     /locus_tag="VFK23_06340"
     CDS             8238..>8733
                     /locus_tag="VFK23_06340"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET7318737.1"
                     /translation="MPYYEFTITIADGFKDRLIKKLMDSGCLGVIDQDKSFVAYFPAT
                     ADVKTIENELALVKTLLGTSGAGPEPVFASRLIPDQDWNESWKKSFKPVDAGRLFTIL
                     PPWEEKRKDRINLIIDPAIAFGTGHHETTRSCLVLMEKYAADSGTKSFLDVGTGTGIL
                     AIAAA"
BASE COUNT         1767 a         2479 c         2408 g         2079 t
ORIGIN      
        1 aagccgttct tcttgatgac ctcggcggcg gcgacgctgt ccgtgaaggt cgtcacgtcg
       61 atgccctcta tttcgagaat cgccctgacc gcctcgcaca cgctcgcttc gtcgtcgacg
      121 acgatcgatt taaacttgtc catggatagg caacctcacc gtaaaagtcg ttcccttccc
      181 ctcggaactc gcgacctcga tatcgccgcc gtgtttcttg atgatgccgt agctcacggc
      241 caggccgagc cccgtgccct tgaatgcctc cttggtggtg aaaaaggggt cgaagatctt
      301 gtggatgtgt tcttccggga tgccgcggcc ggtgtcggaa aaaacgatct ctacggcgtt
      361 tccgtttttg cgcgtgacca cttcgatcct gcccggcggc gtaatggcat gtatcgcgtt
      421 caggagcatg ttcatgacga cctggccgac ctgggcgggg tcggcctcga cgtcgggaag
      481 gttttcgtcg agtttcatcg ccagttcaac gcccttgaag gcatcctggg ccgtgagtga
      541 gtccgcgatc tcggaaacgg gccggttgat gttgatcatc tcgggcttga tgacgatctc
      601 gcgtccgaag ttgaggagct ggcgcacgat gtttcgcgcc cggttggttt cgcgcgccat
      661 gagcttcagc ctctcgcgcc gggggtcccc ggaagggcac tgctccagca tgaggttcga
      721 gaaggtcagc accgcggtca gcgggttgtt gatctcgtgg gcgatgccgg cggcgagttt
      781 tccgaccgag gcgagttttt ccgcttcgac gagctgcgcc tgtttctccc gcagcttctc
      841 gatcgtgtgt tccagcgacc agagcgcgtt cttgagtttc tcctccatca ggttgaacga
      901 cgcctcgagg gaggagagct cgtcctgtcc cttcacctcg atctgttctg agaagtcgcc
      961 ctgggagatt ttcttggtta ttttttcgag cgtcctgatt ggcgtcgcga tgctggtcgc
     1021 gagtttgatg ttgataatga ttccgaggac cacgatcagc accagggcga ggagcagcag
     1081 gttgacggac atcctgatca tcgtggcgat gtcggagcgc tcccgtctcg aaaggttctg
     1141 ggtgaaggac tggatctccc gggcagtctg ccgtagtttt tcgatcgcct ctttttcctc
     1201 ctcgtagagg cggtacagtc cggcgacccg ggcctgataa ttcccgatct cgggcacgga
     1261 tcggagggcc ggcgcggcga gcgccctctc gaacacggcg aaggcgtccc tgttccggta
     1321 cagcatgacg ttcttctcgt agcggcgaac gatgagaaag gcgcggagcg aagcgccgga
     1381 aaggcggcgc tccacggtcc gtccctcttc ccggacgagt tcttcgaact cttcctccga
     1441 ggagaacttt ttggaaacgt tcctgatcag gccctcataa ccggcaatgg ccgatttcat
     1501 catcgtgtaa ttgtcgctgc cgatctcggt cacgatttcc gcgtggatcg cgtcgatgtt
     1561 tgttttcagg gtatcgagat atttctgcag ttcttcataa ctttcccggt ctttgaacag
     1621 gagaaaattc ttttcgtagc ggcggacttc gaggatggtg ttggtgatgt catccgccgt
     1681 ctcgacgagg gtgagccgcg tgccgatcgt gcgaagttct ttataggcga agaagccgag
     1741 gatgacggcg agaaaaatgt agaggccaaa acccagaaaa agtttgtccc gtattttcat
     1801 gataaccgca cggccccttg attcccgcgt tgcgcgtgaa tgaaaggggc gaaccgcgag
     1861 gtccgcccct gtgcccgcgt gtcgttcacg cgtttacctt accatatttt cctgaacgct
     1921 gcaaagggaa taatgaatcg gatacttgat tcatcctgca tcgtgaatct tggatcttgg
     1981 atcctgcctt ccgaccctca cgcctccttc ttcaccttcg ccttcttcat ggtgaggccg
     2041 aatccttcga acatgtagaa aatggcgtat ccccaccaca tcgtgtagaa cacgtagaag
     2101 acgagcaggc ccgagagcat gccggcgtga tcgctcaccc tgttcgcgtc gatctggtag
     2161 aagttgtagg caggttcgac cgccttcttg accacggcgc ttaccatctt tgactcggtg
     2221 atcttctttt ccttgtcgaa ttccttgttc atgcgggaga gggcgttatg ccactgccgg
     2281 aacattttct tttcgtcgtc cgtgccgtag cgggccctga ttttctcacc atcgtttttg
     2341 aacatggcat cggcatcctg gagcgccgag gcgagcacct gcccgaggtc cccctcgatc
     2401 ttcagcgtgg agccgcttat gtccaccttc gccccggccg tggtaaagac cttcgcgatg
     2461 ttcgctgcgc gcgtttccgc gtcaccgggc ttatcctccg gcttgtccgt cctgatggaa
     2521 accgtgaacg gtttacccat gaactgctcg ttgtccttga cgaccttggg aatgaagtag
     2581 gacgatcctt tcgcaagctt gttgaagctg ttgtcggcga attcgaggcc gttcttcccc
     2641 tggaagaggg gcgagaaaat agcgatcagg acccccatga acgtgatggc gaacagcgcc
     2701 ccgatattga acgtctttgc attcttgatg atcatgttat acctcctcct ttacgagcac
     2761 cggctccacc ttctcatctt cgccccggag cacgttgatg ttcatgacaa acttgctgat
     2821 cacccaggcg gcgaaggcgg cgaccacgat ccagaaaata acattgccga acgtctcgat
     2881 gcccttcacc acccccggcg acatgttcac gacctcgagc tcgacgagct tcttggggag
     2941 cgtggcagcc cggttgatga agccggcaag gatggacacg gcatagaagc cgcggatatg
     3001 gatgcccttc acgaccttgg tcgtgagcgc gccgacctgg atgccgatga gagagcccag
     3061 gagcatgccc atggcaagcg tgtagaatac gtatccgtag atggcatact ggccgatcgc
     3121 cgccagccct gccgtgaaga tgatctggag aatgtcggtg ccgaccgtgg tcatcgagga
     3181 gacgccaaag acatagacga acatggggaa cgtgatgaac ccgccgccca cgcccatgat
     3241 ggccgccatg actccgacga cgacgccgcc cagggccacg atcacgccgg agatcttcct
     3301 cccacccttg cagtaatcct cgtcaaagca gatcgccggc ggaatgttga tgttctggac
     3361 cttcttcgaa agctcggtca tgcccgacga gccgccgtgc gcgtcgccgg tgtccttccc
     3421 cttgcgggac ttcaggaagt ccgcgagggc atagaagccc aggaacccga gaaggacggc
     3481 gtagaccaga ctgatgaagg cctcggaaag gagcgggtcc ttgtcataga gcgctttgtt
     3541 cagcgcacct ccgatgaagg tcccgccagc ggagcccacg aggaaggcga cggcgagttt
     3601 gaccgataca tttccgagtt tcttatggac cgacgtgccc atgatcgcct tggcgaaaat
     3661 gtggaagagg tccgtgccca cggcgaggat gcccttgacg cccgcagcca tcagggccgg
     3721 ggtgatgatg aagcctccgc ccgcgccgat gcagcccgtg atcaggccgg cgcagaggcc
     3781 gatggcgatg gaaacgagaa agatgttcgt cgagtagaag gcaggcgcat aggcagtctt
     3841 gctgccgatc atgtcgctcg cttcggccag cattacgagc gcgatcggca gaagcgcggc
     3901 aagcaggatc agccacttct tcctgttctt gataatgctc atcgagacct cataatccca
     3961 tcgtgcatgg gcctccgaag acatcttcaa aaactcaaaa actcttcgtg ctaagttcat
     4021 gataccctcc tttaacttta gtatgattta tatctgtaaa ataagacaat tttgtaagtt
     4081 aatttggccg gcaataaggc cccatcaaag tcattcccaa accgtccggt cgaggatcca
     4141 agcatcgctc ccgggaaacg gtgtttagaa aaaatctccg gattccgact tcggaatgac
     4201 tttaaaaaag agacaagact acccctactt cccggctctt tccatggccg tctttttcac
     4261 tccttccgtt tgcaagattt tttcttcctt tgcatcatcc gatagtctgc tcgcttcatc
     4321 gtattcaccg gcttctgcga acgttatggc aaccatgaga tcttcaatct tctttttgag
     4381 tttgttcatg atatcctccg ttcgtgtttt ctacttccct aactattaca aacgatatgc
     4441 catcgtataa caggttgata aacaaaacat ataattgcta taatttaata tggtgcaata
     4501 atatattgcg taataaaata aaaaagccac gcaaagcgtg gctattggtg caacattata
     4561 tggcattaaa attattgtgt tgctatttta tattgtttga tttttctctg gagctgctgc
     4621 cgaaagattc cgagggctgc tgctgcgcgg gaaatatttc cattatgttc aaagagagct
     4681 gacacgattg cctcccgctc cattttcgac agggtgtctt taagacggag cgctgaggcc
     4741 ctggcagccg gtcgcacagg cgagagccgg aggttctcgg gcaggtccga aggaagtatc
     4801 tccgaggtat tggcgaagga gatggcccgc tcgatgatgt tctcgagttc ccggacgttg
     4861 ccgggatagg aatgctcgag caggatgctc atggcctcgc cgctgatgca ttttatcagg
     4921 atgtcctttc gcttcggcgc atgcgcctgt acgaaatgct ttgccaggag cggaacgtcc
     4981 tccagccggt cgcggagcgg cggcagatgg atattgatga cgttcaggcg gtaaaacagg
     5041 tcttcacgga acaacgcctt ctggcaggag gccttcaagt cccggttcgt ggcggcgatg
     5101 aaccgcaggt cgaccttgag ctggcggctg ccgccgatcc tcataatctc gccttcctgc
     5161 agtacccgca gcaccttggt ctggaacagg ggggaaacat ccccgatctc atcgaagaag
     5221 acggtgccgc cgtccgattg ttcgaggagc cccttcttct ggtactgcgc gccggtgaag
     5281 gcccccttct cgaacccgaa gagctccgat tcgagcagtg tatcggggat ggcgctgcag
     5341 ttgatcgtca tgaagtactt atcgttccgc gggctccagc ggtggatggc cctcgcgatc
     5401 agttccttgc ccgtgccgct ctcgccggtg atgagcacgt tgctctcgct cgccgcggcc
     5461 ttcttgatta gcccgaagac ctcctgcatg gaggcgctgt tgccgatgat ctcgtcgaat
     5521 ccgtatttat tgctcaggat ctttttcttg tggcgggcaa tgacttcgcc gagggattcg
     5581 acggtcaggg gtttttccag gtagtcgctt gctccgagcc gcagggactc caccgccttc
     5641 ttgagatcgg atcgcgaaaa ggcaatgacc ggcgtgctgt gccgtatctc caggagccgc
     5701 tcctgccagt tcgcggtatc caggtcgatg aagacgatgt cataggctgc gtcatcgggg
     5761 gcgtctgacc cgtgcgaagc ggcgtccctg ttcgggtcct ggatatcggg cgagaagccc
     5821 gtcgaccgga agagcgcgta gagatccttc gcttcgtcgg atatgatgag gatgttcagc
     5881 atactaacgt aaaataaagt ttcgcgttgc gcgtttgact cgaaacccga aacccgtatt
     5941 acctgctttt caccaccagc acgggacagg acgcaagcac gatcacccgt tcggcgacgc
     6001 ttcccatgag gagccgctct atgccggtcc tgccgtggct tccgagcacg atgagctcgg
     6061 cgcgcctctc ccgggcgatc tcgataatgg cgtcggcggg cttgccgctc atgatcagcg
     6121 ccttcaccga gacatcctct ttctgcgagg catccttcac cgcccgggcg ttcttctccg
     6181 cttcttgcat ctccttttcc gcgataagct ccctctggct taccgtgaag tcgatatcaa
     6241 ccggcgtcgc gatctccgag gggaccacgg aaacgacggt cagcatgctc ccgttccgct
     6301 tcgcgaggcc gatggcctcg gacgccgccg ctacggagtg ctttgagccg tccgacgcga
     6361 ccacgatgtt cgaaaacccc agccgcgcgg ccctcggaac gacgagtacg ttgcagggcg
     6421 cgtggccgat gacccgcgcc gtgacgctcc ccatcatgag ccgcttcaac cccgtccggc
     6481 cccttctacc catgacgatc acggagcttt tgtccttcac cgcctcgtcc acgatgtatt
     6541 gataagcgtc ctcgccttca cggatgacgg tactgcaggc gaccccttca ttttttgcgc
     6601 gggcctgcac ggcgtccagg ttctgcctgg cctctttccc cttcttttcc acgatctggg
     6661 gcgccagcgt atctaactcc gggttggtgt cgatgacgga cagcgctgtc aaatgacttc
     6721 cgcatttcct ggccagcccg atcgcctccc gaactgcccc ttcgctgaat tccgagccgt
     6781 ccgtggagag aaggaccctt tcaacccctg ctgtaggaca gatttccgcc atgcaattaa
     6841 ccctcccatt gaagttgatc agccctgatg agtctttcgt atagggctaa agtattatgg
     6901 aaattatgtt gcactctgca atactttaat gcatgatagt atctctctgg ctttctgtca
     6961 aacaaaatta tctcgaagct ttggcttgat aaataatttg aaatgtggcg aatagagggt
     7021 aattttaagg gcagtaccgt gtcatgtgtt tgttctcata tgagatccga aggctgttca
     7081 tgagggaaat cttcgtgcgg cgcgtgcccg cgccctggca gtctcttctg cgcggttcct
     7141 gggaggcgcg tccgtctcaa gcttgttcag aaggctcctg caaaccgccg agaccccgtc
     7201 aaccgcctca agaaatgcgg cttcgttagc cttcgatggt ttgttgtagc cgcttatttt
     7261 tcgcacgaat tgcagtgcgg ccgtgcggac ctcttcgtcc gtgaccggcg gatcatagtt
     7321 gaagaggggc ttgatgtttc tgcacatagg cgttacctct cttgcagcga gatcggttga
     7381 attgtaaagg cggacagctt gtcaagcggt tattcactgg gccgcgtccc cgaagtcctc
     7441 caatacaaat cagccgcgag tttacgaggc tgctgcttat acgtttgtca gtcccagagc
     7501 ccgcttcacc cgattcacaa tctctccagg agactctgta atgtcgacca ccaccgactg
     7561 tgcgggctct tccaaaatat cgaattgact ttccaggagg cctgccttca tgtaatgccc
     7621 gcgccgctct gacatgcgct gccggatgag atcgcgggat cccttcaaat atacgaatcg
     7681 gacatcggta ctccgtctcc cgacgaggac atcgcggtat gcttgtttga gcgccgagca
     7741 ggcgacaacg gcagatcgcc cttccccgtc cagttcccgt atgagctgag acagcatatc
     7801 gagccagccg gaacggtctt cgtctgtcag cgcgctgccg tgcgtcattt tttgaatgtt
     7861 ggccggaggg tgatagtcat ccccctcgta aaacggccac cccagctcgt cagcgagaag
     7921 ccgcccgacg gtcgtcttgc cggagccgga aacacccatg acaatgatga tcacgactaa
     7981 tccttatgaa ctgcgcaagg agaacggaaa cattttgtcc ggtaaattta actcgatgcg
     8041 aatgagtcta tcagaaacgg acttttgtgt aaaatgaaat tgacattaac tcattgaaat
     8101 aactatactg gcatgtcacc ggtcagggca aaatgagtgc aaatcggatt tcgaggagat
     8161 agggggagca atgtgcctga tagtatctcg cggcggtttt tttgttcatc tatcatgcta
     8221 tctgaggttc taacgccatg ccctattacg aattcaccat aacgattgcg gacgggttca
     8281 aggaccgtct gataaagaag ctcatggact cgggctgcct gggcgtgatc gatcaggaca
     8341 aatcgttcgt cgcctatttt ccggcaaccg ctgacgtcaa gacgattgaa aacgaacttg
     8401 ctctcgtcaa gaccctgctc gggacttcgg gcgcgggtcc cgagccggta tttgccagca
     8461 ggctgattcc ggaccaggac tggaacgaat catggaaaaa gagtttcaag cccgtggatg
     8521 caggcaggct cttcacgatc cttccgccct gggaggaaaa aagaaaagac aggatcaatt
     8581 taatcatcga ccctgccatc gccttcggca cggggcatca tgaaaccact cggtcctgcc
     8641 tggttctcat ggaaaagtat gcggcagaca gcgggacgaa aagttttctc gacgtaggca
     8701 ccggcaccgg gatccttgcc attgcggctg cca
//
LOCUS       DASSLD010000352         2846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1500711, whole genome shotgun sequence.
ACCESSION   DASSLD010000352 DASSLD010000000
VERSION     DASSLD010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500711"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..942)
                     /locus_tag="VFK23_06345"
     CDS             complement(292..942)
                     /locus_tag="VFK23_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7318738.1"
                     /translation="MAKILIVDDDKEIRANLAEIMADAGYETQQAASGRDAVEKAAND
                     DFDVVLLDLVMPKMSGSDVLGELKRVSPRSKVIMITAFATIENAVDAVKRGASDYLTK
                     PFKIDDILMRVRRVLEEARVDVSAEKRELDCTMSALSNPIRRTIMRLLTQRNSMRLME
                     LVRELGIEDHTKVIFHLKILREAGMVQQDRDKSYSLMQEGVKALDCLNVLEKYFPA"
     gene            1291..1575
                     /locus_tag="VFK23_06350"
     CDS             1291..1575
                     /locus_tag="VFK23_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HET7318739.1"
                     /translation="MPHYVILSNLTDEGRRTIKQKPERILEVNREIEAMGVKIQKQYA
                     LLGSYDFVNIVEAPDNETVMRMSVELGSRGSIKLVTLPAIPVEEFIKKIK"
     gene            complement(1593..2450)
                     /locus_tag="VFK23_06355"
     CDS             complement(1593..2450)
                     /locus_tag="VFK23_06355"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HET7318740.1"
                     /translation="MRIRTFIAPIALSFLGLLVLTAAVDVDERGDVYRLSDKKTISFS
                     HMVQDVKKADFIVVGEVHDIPEHHQLQLDIIRAFHESGAPLAIGLEMFRTESQNALDS
                     WVKGVLPVHRFISVYYENWRMAWPLYRDIFTYAHDHQIPMLGLNLPSSITELVARQGF
                     SSLKEKEKKQLPPGISCDIDPTYMEFIKRAYAAHSQKTDERFVHFCEAQMVWDKSMAW
                     RLIEYRKTHPDQKVVVLAGVGHAWKRGIPEQIARGSKYAVKVVMPIVPDEIDEDTVSI
                     RDTDYLLLR"
     gene            2635..>2846
                     /locus_tag="VFK23_06360"
     CDS             2635..>2846
                     /locus_tag="VFK23_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318741.1"
                     /translation="MRLSFKYMISAFILALPFSAFAEDLGLVRLSLIEGDVQVLIQDT
                     TDWTEATINLPLNERDRVWVDGDGKA"
BASE COUNT          680 a          730 c          757 g          679 t
ORIGIN      
        1 gggaaccgaa ggcctgaaaa caatgtctcc gccccccacc cgctgcgcca acaggtttag
       61 cggcagcgaa agaccgataa gaactatgac aacgaacaat tgcttcatgg aatagcgccc
      121 cggattaccc ggtaagctta aaaaggcgtc ggctcggtac gcagaaatcc caacattgtt
      181 ataacgcttt ttcgggattt caacaatgaa atttttgccg tatgggccgg gaggtacaac
      241 ggtatcgggc tgatattcgc aaaaaaggag aacgggggaa tgagagaccg gtcatgccgg
      301 gaagtatttt tccagaacgt tcaggcagtc aagggctttt acgccttctt gcatgaggga
      361 ataggacttg tccctgtctt gttgcaccat ccccgcttcc ctcagaattt tcaggtggaa
      421 gatcaccttc gtatgatctt caatgccgag ttccctgacc agttccatca ggcgcattga
      481 gttcctctgc gtaagcaggc gcatgatcgt cctccggatg ggattggaca gggcgctcat
      541 ggtgcagtct aattcccttt tctccgcgct gacatcgacc cgcgcttctt caagaacgcg
      601 gcgcacgcgc atcaggatat cgtcgatctt gaacggtttg gtcagataat cgctcgctcc
      661 ccgcttcaca gcgtcgaccg cgttctcgat ggtcgcaaag gccgtgatca tgatgacctt
      721 tgaccgcggg ctcaccctct tgagttctcc aaggacgtcg gacccgctca ttttaggcat
      781 gaccaggtcg aggaggacca catcaaaatc gtcgttcgct gccttttcaa ccgcatcgcg
      841 gcctgaagcc gcctgctgcg tctcatagcc cgcgtcggcc atgatctcgg cgaggttggc
      901 cctgatttcc ttgtcatcgt ctacgatgag aatttttgcc atggtgcctc gcgattcgga
      961 gagttccggg cgggatgcaa gcttcgccgc ttccccgtac ggtccttgtt gcaattaagc
     1021 agactacctg atgttacggt actaactgta tgatatcaag aacgagcttg caagtgaatt
     1081 tgatttttcc ttaattgatc atttgtgaaa ttaacttcac atcccgttcg gaaaggggta
     1141 gaaaaaaaat ggatacttac gccgtaatgc taatttttcg cagctcggca gtaatgaggc
     1201 tataatagta aaaaaacatg gaaaacggaa aaagaaccaa agtattaaac cccgtacata
     1261 ttaatctcga tcaaaaagga ggaacttgcc atgccgcact atgtgattct cagcaatctt
     1321 acggacgaag gcagaaggac gatcaaacaa aagccggagc ggatcctgga ggtcaaccgg
     1381 gaaatcgagg ccatgggggt aaaaatacag aaacagtatg ccctgctcgg ctcctacgac
     1441 tttgtcaaca tcgtcgaggc accggataac gagacggtga tgaggatgtc ggttgaatta
     1501 ggctcccggg ggtcaatcaa gctcgtgact cttccggcta ttccggtcga ggaatttatc
     1561 aagaagataa agtaacgggc tgcatgcggt ctttatcgaa ggagcaggta gtcggtgtcg
     1621 cggatcgaga cggtgtcctc gtcaatctcg tccggtacaa tcggcatgac gaccttcacg
     1681 gcgtatttcg agccgcgcgc gatctgctcc ggaattcccc tcttccaggc gtgcccgacg
     1741 ccggcaagga ccacgacttt ttgatcgggg tgcgttttcc gatattcgat caggcgccac
     1801 gccatgctct tgtcccagac catctgcgct tcgcagaaat ggacaaaacg ctcgtccgtt
     1861 ttctgcgaat gggccgcgta ggcgcgtttg atgaactcca tgtaggtcgg atcgatatcg
     1921 cagcttatgc ccggcggaag ctgttttttc tctttttcct tgagcgaaga aaacccctgc
     1981 cttgccacca gttcggtgat gctgctcggt aaattcagcc cgagcatggg tatctgatgg
     2041 tcgtgggcat aggtgaagat gtcgcggtac agcggccatg ccatgcgcca gttttcgtaa
     2101 tagacggaga taaaccggtg gacgggcaga acacctttga cccaggaatc gagggcattc
     2161 tggctttccg tccggaacat ttcgagcccg atagccagcg gggccccgga ctcgtggaat
     2221 gccctgatga tgtcgagttg cagttggtgg tgttcaggga tgtcatggac ctcgccgacg
     2281 acgataaaat ccgctttctt cacgtcctgg accatgtggc tgaaagatat ggttttctta
     2341 tcgctcagcc ggtaaacatc gcccctttca tcaacgtcaa cagcagccgt gaggacaagt
     2401 agtcctaaaa acgaaagggc aatcggagca ataaacgtcc ttattctcat aatttaattg
     2461 ttaccacacc gggagtgatt gtcaagacgg ctgcgaaggg ataaaatata gctggtttga
     2521 aatgcaacga taacggctgg acattgtcta ataaagtaga atcacagagt ctgtggcata
     2581 agtccctttt tcacccttcg accagcgtgc cgacgtactc aaaggaggtt cgttatgcgt
     2641 ttgtctttca agtatatgat atccgctttc atcctggcgt taccgttctc cgccttcgcc
     2701 gaagatctcg gcctcgtgcg attaagcctg atagaaggcg acgtgcaggt ccttatccag
     2761 gacacgacgg actggaccga ggcgaccatc aatctgccgc tgaatgagcg ggaccgcgtc
     2821 tgggtcgacg gggacggcaa ggcgga
//
LOCUS       DASSLD010000353         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1372958, whole genome shotgun sequence.
ACCESSION   DASSLD010000353 DASSLD010000000
VERSION     DASSLD010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372958"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>2062
                     /locus_tag="VFK23_06365"
     CDS             18..>2062
                     /locus_tag="VFK23_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HET7318742.1"
                     /translation="MMERYICIHGHFYQPPRENPWLEAIEIQDSAHPYHNWNERIMAE
                     CYAPNSAARLLDGDQRIIDISSNYTKISFNYGPTLLSWMESAAPDAYQTILEADRQSR
                     ERFSGHGSAIAQAYNHVIMPLANGRDKKTQVLWGIRDFERRFQRAPEGMWLPETAVDL
                     ETLNIFAEAGIKFTVLAPHQASRIRAVGSEEWEGVSGATVDPTRPYLCRLASGKEIVL
                     FFYDGPISRAVAFERLLTQGEDFAARLMGGFSDERQWPQLLHIATDGETYGHHQKFGD
                     MALAYALHHIETGGLAKLTNYGEYLAKHPPDREAEIFENSSWSCIHGIERWKSNCGCN
                     SGGRPGWNQEWRAPLRRALDRLRDDLSIRFEEGGRQYLKDPWLARDEYIEVVLDRSEG
                     NVDAFFKRHAVRELGEVERSNALRLLELQRHALLMYTSCGWFFDELSGIETVQVMQYA
                     AQSIKLWEGLSGENLEAGFLDVLKEAKSNVSDHGDGARVYEKFVRPVMVDLKKTAVHY
                     AVSSLFEDYGDRTAIYCYDVLREDYQRTDAGRTKLAVGRASVISGITKESERVGFAVL
                     HLGNHSLNCGVGSFQGDDPYQAMKQEIGAAFESGDFADVVRLMDKHFGVNNYSIRDLF
                     RDKQRMVLNLLIDSTREDLETSYRRMYDDSRALMGFLRETGMPLPKAFLAVAEFILNT
                     "
BASE COUNT          433 a          604 c          645 g          380 t
ORIGIN      
        1 tccgctggga aggaaaaatg atggaacgat atatctgcat ccacggccac ttctaccagc
       61 cgccgcgtga aaacccctgg cttgaggcga tcgagatcca ggactcggcc cacccatacc
      121 acaactggaa cgagcgcatc atggcggaat gttacgcccc gaactcggct gcgcggctcc
      181 tggacgggga ccagcggatc atcgacattt cgagcaatta cacgaagatc agctttaact
      241 acggccccac gctcctgtcg tggatggaga gtgcggcgcc cgatgcgtac cagacaatac
      301 tcgaagcgga ccggcagagc agggaacggt tttcgggcca cggcagcgcc atcgcccagg
      361 cgtacaatca cgtcatcatg ccacttgcga acggccgcga caagaagact caggtccttt
      421 ggggaatcag ggatttcgag cgacggttcc agcgcgcccc cgaaggtatg tggctgcccg
      481 agactgcggt ggacctggag acgctgaata tttttgccga ggccgggata aaattcaccg
      541 tcctcgcgcc ccatcaggct tcacgcatac gggcggttgg cagtgaagag tgggaaggcg
      601 taagcggcgc cacagtcgac cctacacggc cctatctgtg caggctggcg tccggcaagg
      661 agatcgtcct gttcttctat gacggcccga tctcccgtgc cgtggcgttc gaacggctgc
      721 tcacccaggg agaggacttt gcggcccgtc tcatgggagg attttccgac gagagacaat
      781 ggccccagct ccttcacatc gcgaccgatg gagaaaccta cgggcaccac cagaagtttg
      841 gcgacatggc gcttgcctac gcccttcacc atatcgaaac cggcggcctc gcgaagctca
      901 cgaattacgg cgagtatctc gccaagcacc cgcccgaccg tgaggcggag atcttcgaga
      961 actcatcctg gagctgcatt catggcatcg agcgctggaa aagcaactgc ggctgcaact
     1021 caggcggccg tcccggctgg aaccaggaat ggcgagcgcc gctgcgccgg gcgctggacc
     1081 ggctccggga cgacctctcg atccggttcg aggaaggggg gaggcagtac ctgaaggacc
     1141 cctggctcgc gcgggacgaa tacatcgagg tggtcctcga ccggtcggag gggaacgtgg
     1201 atgcgttctt caagcgtcac gccgtgcggg agctcggcga ggtagagcgg tcgaacgcgc
     1261 tcaggctgct cgagctccag cgccatgccc tgctcatgta cacaagctgc ggatggttct
     1321 tcgacgagct ttcgggcatc gagactgtcc aggtcatgca gtacgcggcc cagtcaatca
     1381 agctctggga aggcctttcg ggcgagaacc tggaagcggg ctttctcgat gttctcaagg
     1441 aagcgaagag caatgtttcg gaccatgggg acggtgcgag ggtatacgaa aagtttgtca
     1501 ggcccgtgat ggtagacctc aagaaaacgg cagtccacta tgccgtgagc tccctgttcg
     1561 aggactacgg cgaccgcacg gccatttact gttacgatgt cctgcgggag gattatcaga
     1621 ggacggacgc agggcggacg aagctggctg ttggccgtgc ttccgtcatt tcgggcatca
     1681 cgaaggaatc tgagcgcgtc ggttttgcgg tcctccatct cggcaatcac tcgctcaatt
     1741 gcggcgtggg ttcgtttcag ggagacgacc cgtaccaggc catgaaacag gagattggcg
     1801 cagcattcga atcgggcgac tttgcggacg tcgtcaggct gatggacaag catttcggcg
     1861 tgaacaacta ctcgatccgg gacctcttcc gggataaaca gcggatggtg ctgaatctgc
     1921 ttatcgacag cacgcgggaa gatctcgaga cgtcctaccg ccgcatgtac gacgacagcc
     1981 gggcgctcat gggattcctc cgggagacgg gtatgcctct gccgaaggcc ttcctcgctg
     2041 tcgccgaatt cattctgaac ac
//
LOCUS       DASSLD010000354         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1378290, whole genome shotgun sequence.
ACCESSION   DASSLD010000354 DASSLD010000000
VERSION     DASSLD010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378290"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(356..685)
                     /locus_tag="VFK23_06370"
     CDS             complement(356..685)
                     /locus_tag="VFK23_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET7318743.1"
                     /translation="MKTHRYAAAAIVIVLLTAFAAHAEDPMKRSTGEYIDDKAITAKV
                     KAALLKDQEVKGLQVKVETYNGVVQLSGFVDKPEQISRAGEVAKGVEGVKSVKNDLNL
                     RSKPGKY"
     gene            complement(878..1594)
                     /locus_tag="VFK23_06375"
     CDS             complement(878..1594)
                     /locus_tag="VFK23_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7318744.1"
                     /translation="MLTDQRVMSLSSLRHKIALFHSLEPADFQLLEKRFVRRRYPGGQ
                     VLFHMGDEGGSFHIIVRGRVKVTIPSSSGEELILAILATGDLLGELSLFDGKPRSATV
                     QAVEETETLCLHREDLMALMQKRFGIVEKVLEVLARRIRDTDRQLAESRFLDITARLA
                     KKILDLGDAFGVQEDGRTRIGVKVTQKDLASMIGATRESVNKQLKALRTQGLIRIDEG
                     TIEIRNRESLEQKARALSRI"
     gene            1770..>2024
                     /locus_tag="VFK23_06380"
     CDS             1770..>2024
                     /locus_tag="VFK23_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318745.1"
                     /translation="MTDGATILAEIVDAGKPKLVLGEVHRTLDGIVEKDDLHRLDRVF
                     SEVQRLFAGRYPGYSKCKTRYHDLEHTTDTLLAMARLIHRA"
BASE COUNT          413 a          575 c          592 g          444 t
ORIGIN      
        1 gttcacgtgc agatgaaggt ggaaaccgta tgtcacgagg tccctcctgg tatatatata
       61 aattcccggc gagcatacca cgacgctgat acacccgaat acgtgaaccg gagctccgat
      121 gccttgcggg ccttgtccgg aaggcagtac aggcggcgcc gagggaaata accggttgga
      181 acggggcgtg tgattttctc gatgaaacgg ggagaagctc aggggtgttt cacggaaggg
      241 aaacggtcag cttcttcgct tcccgaccgt ggcagcttct cccccttaag gactcaagtg
      301 aacgtgagct acacgcaaga gcctcaccga tctacccgat ggggctcttg cgcgatcaat
      361 acttgcctgg tttcgaccgg aggttcaggt cattcttgac ggatttcacc ccttcaacgc
      421 ccttggcgac ctcgcctgcc cgggaaatct gttcaggctt gtcgacgaat ccgctcaact
      481 ggacgacacc gttgtatgtt tccaccttca cttggagccc cttgacctcc tggtctttca
      541 gaagggcggc tttgactttg gctgtaatgg ccttgtcatc gatatactcc ccggtgctcc
      601 gcttcatggg atcttccgca tgggcggcaa aagctgtgag caacacaatg acaatggcag
      661 ccgctgcgta gcgatgtgtc ttcatcacag gtttcctcct ccgcgaaatg tttaagaatt
      721 tgcgatcccc tccgcccccg ttgcagcagc gtaaacattc gttcctgccg accccctctg
      781 caggaaaccc gcggcaaacg gggacttcaa gctatattga ttcgcgatcg acggcgaaag
      841 tttccgtgcc gggtgtagag ttgggtcgtg gcaagattca gattctcgac agggcacggg
      901 ctttctgctc gaggctctcc cggttccgga tctcgatcgt tccttcgtcg atccggatga
      961 ggccctgggt acgcagcgcc ttgagttgct tgtttacgct ttcccgcgtc gctccaatca
     1021 tggaagccag atctttctgc gtcaccttca cgccgatccg ggtccgtcca tcctcctgga
     1081 caccgaaggc gtctccgaga tcgagtatct tcttcgccag gcgtgcggtg atgtcgagaa
     1141 agcgactctc cgcgagctgc ctgtccgtgt cgcggattct gcgggcaagg acttccagca
     1201 ctttttcgac gatgccgaaa cgcttttgca tgagggccat gagatcctca cgatgcagac
     1261 agagggtctc ggtctcttct acggcctgaa cggtggctga gcggggcttg ccgtcgaaga
     1321 gggagagctc tccgaggagg tcccccgtcg ccaggatggc caggatcagt tcctcgccgg
     1381 aggaggacgg gatggtgacc ttcactcgcc cgcggacgat gatgtgaaag gagcccccct
     1441 cgtcgcccat gtggaagaga acctggccgc ccgggtaccg cctccggacg aatcgtttct
     1501 cgaggagctg gaaatcggcc ggctcgaggg agtggaacag cgcgatcttg tgtcgcagac
     1561 ttgacaggga catgaccctc tggtccgtga gcatggattc gctgccgagg cgaaaagggt
     1621 aacaccccca cttggaccgg tcaatttccc gggggatatt tcacaatcgc cgtgtgaagt
     1681 ggttcatacc ggttcgacgg caaacgtcgt attatttatt aattagtacg cggtatcgat
     1741 ggaaaaaggt agggggagag ggagcgatca tgaccgatgg ggcaacgatt ctcgcagaga
     1801 tcgtggatgc ggggaaaccg aaactggtgc tcggagaagt ccaccggact ctcgacggaa
     1861 tcgtggaaaa agacgatctg caccggctcg accgggtctt ctccgaagtt cagcgtctct
     1921 tcgcgggaag gtatccggga tactccaaat gcaaaacccg gtatcacgat ctggaacaca
     1981 cgaccgacac ccttctcgcg atggcacggc tcattcacag ggcc
//
LOCUS       DASSLD010000355         4349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_435811,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000355 DASSLD010000000
VERSION     DASSLD010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435811"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFK23_06385"
     CDS             <1..304
                     /locus_tag="VFK23_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318746.1"
                     /translation="PLLDLFTDKHIVYHYVPDSLPAREEVERSSLRFTWEYRNPAYYS
                     NERRAPGTAPVVVISDASNAPLSKNVLQSIEGRRLAGEFIANENIFQYTVGVRIYH"
     gene            667..2085
                     /locus_tag="VFK23_06390"
     CDS             667..2085
                     /locus_tag="VFK23_06390"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HET7318747.1"
                     /translation="MEEKRNQIFAFAVLGLMIVISLLYYKANVFYMYLVAYIWFGIAY
                     GMMLQYGRFCFASASRDLLAAGVPRMAVGILIALIFFSVIQATLQATNMSTFHPAPFG
                     IHTLLSGMIFGVGMVLAGGCASGSLYKIGEGNGTSILSILGISFGQAIFVDVGGPFLK
                     LVPESWAASSAAKHLPADKVTSWFDYYLSGYVWDKPSIQLSHTQVFSNALPGASKFFV
                     ADALISAIIPALILIVVIYAFYIRKGFLKKRAKQTGQPTGFSDNFAGIWSMLTASKRT
                     SIMGVIIGITAGVHIFVMKGMQIKFGISNFGELLTKMGHAADTSIRGTVFDPGYWYIT
                     SQEGQFGAWVLDKIGWNMRDNVFFGVNNGLPEPWRNPALWMSIGIIAGAMIMSLMSKE
                     FKFKLPKGELIVWGLLGGTLMGLGSRPALGCNIGAFFIRVAGGDPSGWLYGIGMVTGA
                     FAGVKFFNWWSERKMAAEMADF"
     gene            2106..2357
                     /locus_tag="VFK23_06395"
     CDS             2106..2357
                     /locus_tag="VFK23_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HET7318748.1"
                     /translation="MAMKFEKKSEGVYALDVCGYVCPHPQIYCKKSLEKIGEGEVLEM
                     TFDNPSSAETIVQMLDQAGHDLMEKKNEGGKIVFKIKKA"
     gene            2387..2713
                     /locus_tag="VFK23_06400"
     CDS             2387..2713
                     /locus_tag="VFK23_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318749.1"
                     /translation="MKPPLVILIAIMVFILGFLVVYSQQHDMNPNAQTQVAAGGYGSG
                     STHTSGGYGAAPAAASGGYGAAPAAAAGGYGAAPAATPAPAAAGGYGAPAPKPGAQTP
                     AAGGYK"
     gene            2899..4311
                     /locus_tag="VFK23_06405"
     CDS             2899..4311
                     /locus_tag="VFK23_06405"
                     /inference="COORDINATES: protein motif:HMM:NF005548.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7318750.1"
                     /translation="MNGSKKDTIILGGGLSGLSSGYLLTKSGFSVSVYERDAVVGGLS
                     KTVEKNGFRFDLGGHRFFTMDAGVDSLVRELMGNELITVRRSSKIYLRGRFLDYPLKP
                     VSALFGLGLLNTARALADYGWETARRTLRHREVVSLEDWVVGNFGRTLFNLYFKEYSE
                     KVWGLDCGSISASWVAQRIKGLSLAKAVKNAFFKASGRGLATLTDSFLYPSLGIGRLS
                     DRLKEEIEKGNEVFTDSGVASVNRTGFTVNGISIQEHGRMRMAAADEFISSIPVTKLI
                     RILDPPPPATVLDAAEMLKFRDLVVVALMVDRPRVTDQTWIYIPEKKIPFGRIHEPTN
                     WSPAMAPPGKSILVVEFFSFRGDEVWNMPDEMLTDISADHLARLGFINRSEVMDSVVV
                     RVPKAYPLFEVGYQRHADILHDYLGRFRNLHIAGRGGMFRYYNMDVAIRSGMEAAEKV
                     TRRCLSADAAWTDEPVLMRK"
BASE COUNT          973 a         1207 c         1205 g          964 t
ORIGIN      
        1 tccccttctc gacctgttca cggacaagca tattgtgtat cactacgtgc cggacagcct
       61 ccctgcccgc gaggaggtag aacggtcgtc tcttcgtttc acatgggagt accggaaccc
      121 agcgtattac agcaacgaac gccgcgcgcc gggaactgcc cccgtcgtcg tcatttccga
      181 tgcctcgaac gctcctctgt caaaaaacgt tttgcaaagt atcgagggac gccgattggc
      241 cggagagttc attgcgaatg aaaatatttt ccagtacacg gtcggagtca ggatctatca
      301 ttaatatttc gcgaaatttt gcacctgaaa ccgaaaaaaa actggacacg gtcaactgca
      361 aaattaagtt ttttattgca atatcaagca actatgcaac atatagggaa aaataatgct
      421 tgccatggat tattgattgt tctattatat aagcctacag gtaaattgtt tcctagaatt
      481 ttgttaaccg cttttttcag ttcattttta tgttaaccgt gttaaacaag aaaccgatat
      541 ttacattgag cgttaaagtt aacagcgaaa ggggggaagg cactctcaat ctacaataat
      601 aaggcacaac caagataggc ttctgagctt tgtctttcaa cgctatttta agggggaaat
      661 agtattatgg aagagaaaag aaatcagatt tttgccttcg cggtgctggg cttgatgata
      721 gtcatcagcc tgctgtatta caaggccaat gtattctaca tgtatctggt ggcatacatc
      781 tggttcggaa tcgcctacgg tatgatgctt cagtacggca ggttctgttt cgcctcggct
      841 tcaagggacc tgttggccgc cggcgttccg agaatggccg tcggcatcct catcgcgctc
      901 atctttttca gcgtcatcca ggcgacgctc caggcaacca acatgagcac cttccatccg
      961 gccccgttcg gcattcacac actgctgtcg ggaatgatct tcggcgtggg gatggtcctc
     1021 gcaggagggt gcgcctcggg gtccttgtac aagatcggtg agggcaatgg cacctcgatc
     1081 ctctccatcc tcggcatatc cttcggccag gccatcttcg ttgatgtagg cgggcctttc
     1141 ctgaaactgg tacccgaatc ctgggcggca tccagcgcgg caaagcacct gcctgccgac
     1201 aaggtaacgt cctggttcga ctactacctc tccggctatg tctgggacaa gccctccatt
     1261 cagctttcgc acacccaagt cttctctaat gccctcccgg gcgcatccaa attcttcgtc
     1321 gcggacgcgc tcatcagcgc gatcatcccg gccctcattc tgatcgtcgt tatctacgcc
     1381 ttttacatca ggaaggggtt cctcaagaag cgcgctaaac aaacgggtca gccgacaggc
     1441 ttttcggaca attttgcagg gatatggagc atgctgacgg cgtccaaaag gacctcgatc
     1501 atgggggtca tcatcggcat aactgccggg gtgcacattt tcgtcatgaa gggcatgcag
     1561 atcaagttcg gcatctcgaa cttcggcgag ctccttacca agatgggtca tgcggcagac
     1621 acgagcatcc ggggcaccgt gttcgacccc ggctactggt atatcacgag ccaggagggg
     1681 cagttcgggg cgtgggtgct cgacaagatc ggctggaaca tgcgtgacaa cgtgttcttc
     1741 ggcgtcaata acggacttcc cgagccctgg cgcaatcctg ctctctggat gtccatcggc
     1801 atcatcgccg gtgccatgat catgtcgctc atgagcaagg aattcaaatt caagttgccg
     1861 aagggcgagc tgatcgtgtg gggcctcctg ggcgggacgc tgatgggact gggctcgcgg
     1921 ccggctttgg gctgcaacat cggcgcgttc ttcatccgcg ttgcgggcgg cgatcccagc
     1981 ggctggctgt atggaatcgg gatggtaacc ggcgccttcg cgggcgtcaa gttcttcaac
     2041 tggtggtcgg aaagaaagat ggccgcagaa atggccgatt tttaaattat aaggaggaat
     2101 gaatcatggc tatgaaattt gaaaaaaaga gtgaaggggt ctacgcgctt gatgtctgcg
     2161 gctatgtgtg cccccatccc cagatctact gcaagaaatc gctggagaag attggtgagg
     2221 gcgaggttct cgagatgacc ttcgacaacc cttcatctgc ggaaacgatc gtccagatgc
     2281 tcgatcaggc tggacacgat ctgatggaga aaaagaacga aggcggcaaa atcgtattta
     2341 aaatcaaaaa agcctgacca ttcagctcaa atcacaggag gatagcatga aaccgcctct
     2401 cgtaatactc atcgcgatta tggtattcat tctcggtttc ctggtagtat acagccagca
     2461 gcatgacatg aacccgaatg cccagaccca ggttgcagca ggcgggtacg gctccggttc
     2521 aacgcatacg tccggcgggt acggcgcggc cccggctgcc gcttcgggcg gatacggcgc
     2581 agccccggct gctgctgcgg gtggctatgg tgcggccccg gccgcgacac cggccccggc
     2641 cgcagcgggt ggttacggag ctcctgctcc gaaaccgggc gcccagaccc cggcggcagg
     2701 cgggtataaa tagaactttt ccggaagttc atgatcatac gtacgtcccc gttaggccat
     2761 gtcatatacc atctttgccg ttcaaaccct tccctacgtc cgggcagtcc cccccggacg
     2821 cggggaaggt gcaccagcag ggccccgacg tcgtagtctc aaccccctcc gtcatttatc
     2881 ctggatacag gtactgccgt gaacggatcg aaaaaagaca ccattatttt aggcggcggg
     2941 ctctccggtc tttcctccgg ctacctgctg accaaatccg gcttcagtgt cagcgtatac
     3001 gaacgcgatg ccgttgtcgg cggtctgtcg aaaacagttg agaagaacgg attccgcttc
     3061 gatctaggag gccaccgctt cttcaccatg gatgcgggtg tggactccct cgtcagggaa
     3121 ctgatgggaa acgaattgat caccgtccgg cgcagcagca aaatctatct gcgcggcagg
     3181 ttcctcgact atcccctcaa accggtcagt gccctgttcg gcctcggcct cctgaacact
     3241 gccagggcac tcgcagacta tggatgggaa acagcgcgca ggacactgcg ccacagggag
     3301 gttgtttcgc tcgaggactg ggtcgtgggc aatttcggcc gcaccctttt caatctttac
     3361 ttcaaggaat acagtgaaaa agtctggggg ctcgattgcg gcagtatcag cgcctcctgg
     3421 gtggcccagc ggatcaaagg cctgtctctg gcaaaggcgg tgaaaaacgc cttcttcaag
     3481 gccagcggca ggggtctggc cacgctaacg gacagttttt tgtatccctc cctcgggata
     3541 gggcgtcttt ccgaccggct caaggaagag atcgaaaagg gcaatgaggt cttcaccgat
     3601 tccggggtcg ccagcgtcaa ccgcacaggc ttcacggtca acggcatctc catccaagaa
     3661 cacggcagga tgcggatggc ggctgcggac gaattcatct cgagcattcc cgttaccaaa
     3721 ctcatacgca tactcgatcc tccaccgccc gcaacggtcc tcgatgccgc agagatgctg
     3781 aaattccggg acctcgtcgt tgtggcgctc atggtggacc ggccccgggt cacagaccag
     3841 acgtggattt atatcccgga aaagaagatc ccctttggca ggatccacga gccgacgaac
     3901 tggagccccg cgatggcgcc gccgggtaaa tcgatcctgg tcgtggaatt cttcagtttc
     3961 aggggagacg aggtctggaa catgccggat gaaatgctta cggatatctc cgcggaccac
     4021 ctcgcccgtc tcggcttcat caaccggagc gaggtcatgg acagcgttgt cgtacgcgtg
     4081 ccgaaggcct atcccctgtt cgaggtcggc taccagaggc atgccgacat acttcacgac
     4141 tacctggggc gtttccgcaa tttgcacatc gccggcagag gcggaatgtt ccgctattac
     4201 aatatggacg tggcaatacg ctcaggcatg gaggccgcgg aaaaggttac ccggagatgc
     4261 ctttccgcag acgccgcctg gacggacgaa ccagtcctga tgagaaagtg acggtcctat
     4321 gaagtgcgcg ctggtcatcc ccgcctggg
//
LOCUS       DASSLD010000356         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_424161,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000356 DASSLD010000000
VERSION     DASSLD010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424161"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VFK23_06410"
     CDS             <1..236
                     /locus_tag="VFK23_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7318751.1"
                     /translation="EAQASVKEVEKMVVRGVPFVEIIKTAKDKNADMIVIGTHGRTGI
                     DHMLFGSTAEKVVRKAPCPVLAVRIAGKEFKMP"
     gene            complement(392..817)
                     /locus_tag="VFK23_06415"
     CDS             complement(392..817)
                     /locus_tag="VFK23_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET7318752.1"
                     /translation="MVQKASKKLTEMMNQAIAAELQAIVQYMWHHVMVKGMHAESLAE
                     VFEKISIDEMKHAEKIAERLFYFDVNPTTKPNPIATGGDATQMLKADTKAEEEAITLY
                     KDIIKQAASEGDETTRLLFEEILSQEEGHHDTFTTLLGQ"
     gene            991..>2323
                     /locus_tag="VFK23_06420"
     CDS             991..>2323
                     /locus_tag="VFK23_06420"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HET7318753.1"
                     /translation="MGKNLVYKILESHLVAGKLEPGTEIAVRIDQTLTQDATGTMAYL
                     QFEAMNVPRVRTELSVSYVDHNTLQDGFENADDHRYLMTVAAKYGIHYSKAGNGICHQ
                     VHLERFGRPGKTLLGSDSHTPTGGGIGMIAIGAGGLDVAVAMGGGPFYLTCPKVVKVN
                     LSGRLWPFVSAKDVILAVLRLLTTKGNVGTVIEYGGEGVGTLTVPERATITNMGAELG
                     VTTSVFPSDETTRAFLRAQKREADWTPLDADPDAKYDRVIELDLGTVEPLMAKPHSPD
                     NIAAVKELAGTKVDQVMVGSCTNSSYRDLMIVASMLKGRRVFPDVSFGVAPGSRQVLE
                     MIARNGALADMVAAGARILESTCGFCIGAGQAPRTDAVSIRTSNRNFEGRSGTKSAKV
                     FLASPESAVAAALTGGITDPRDLGLPYPEIEMPGLFWIDDTMILSPAKDPLS"
BASE COUNT          470 a          699 c          702 g          452 t
ORIGIN      
        1 cggaggcgca agcgtcggtc aaggaagtgg agaagatggt cgtgcggggc gtgccgttcg
       61 tcgagatcat caaaacggcg aaggacaaga acgccgacat gatcgtcatc ggcacgcacg
      121 gcaggaccgg catcgaccac atgctctttg gcagcacggc ggaaaaagtg gtccggaagg
      181 cgccctgccc ggtgctcgca gtgcgcatag cgggcaagga attcaagatg ccatgagacg
      241 ccgcaggacg ggggaaattc caagcaccaa ggaacaaatc tccaatttgg acacaggtat
      301 ttgggatttg gtgcttgcca ttgaaacata aaaagggcag gctcgcgcct gcccttttgt
      361 tcatcccgtc gtgatcaaac tctctgcgtg gttattgtcc caagagcgtc gtgaaggtgt
      421 cgtgatggcc ttcttcctgg gagaggatct cctcgaacag gagccgggtg gtctcgtcac
      481 cttcggaggc agcctgcttg atgatgtcct tgtagagtgt gatggcctct tcctccgcct
      541 tcgtatctgc cttcagcatc tgtgtcgcgt cgccgccggt ggcgataggg ttcggcttgg
      601 tggtggggtt tacgtcgaag taaaaaagtc tctccgcgat cttctccgcg tgcttcatct
      661 catcgatcga aattttctcg aacacctccg caagcgactc cgcatgcatc cccttgacca
      721 tgacatggtg ccacatgtac tggacgatcg cctggagctc cgcggcaatc gcctggttca
      781 tcatttccgt caacttctta ctcgccttct gtaccatatg ggctgcctcc ttcgtggggc
      841 atgagcgtta ccgtcgcaat ggcataagca tagcacagga catttccgtt ttcaaccggg
      901 gtgacatttt cacaatttca gttgccttgc actggccgtt gaaatataat gtcgcggact
      961 tgaataaaaa atgaaggagc ttttgcaatc atgggcaaga atctcgtcta taagattttg
     1021 gaatcacacc tcgttgcggg caaactcgag ccaggcaccg agatcgcggt ccggatcgac
     1081 cagaccctga cgcaggacgc aaccggcacc atggcgtatc ttcagttcga ggccatgaac
     1141 gtgccgcggg tccggacgga gttgtcggta agctacgtcg accacaatac gctccaggac
     1201 ggattcgaga acgccgatga ccatcgctac ctcatgaccg tcgctgcgaa gtacggcatc
     1261 cactactcga aggcgggcaa cggcatctgc catcaggtcc acctggagcg attcggcagg
     1321 cccggcaaga cgcttctggg gtctgactct cacactccga cgggcggtgg catcggcatg
     1381 atcgccatcg gcgcgggcgg tctcgacgtg gccgtcgcca tgggcggcgg gcccttctac
     1441 ctcacctgtc cgaaggtcgt gaaggtgaat ctctccggaa ggctctggcc ctttgtctcg
     1501 gccaaggacg tgatcctcgc ggtcctccgg cttctcacta ccaagggcaa cgtgggaacg
     1561 gtgatcgagt acggcggcga aggagttggg accctcacgg tgccggagcg ggccacgatc
     1621 acgaacatgg gagcggagct cggcgtgacc acgtcggtct tcccgagcga cgagacaacg
     1681 cgggcgttcc tgcgtgcgca gaagcgcgag gccgactgga ccccactcga cgcggacccc
     1741 gacgcgaagt acgacagagt catcgagctc gacctcggca ctgtcgaacc gctcatggca
     1801 aagccccact cgccggacaa tatcgcggcc gtaaaggagc tggcgggaac aaaggtcgac
     1861 caggtcatgg tcggctcctg cacgaactcg tcctatcgcg acctcatgat cgtcgcttcg
     1921 atgctcaagg ggaggcgggt gttcccggac gtgagcttcg gcgtggcgcc gggctcgcgc
     1981 caggtgctcg agatgatcgc gaggaacggg gcgctggccg acatggtcgc cgccggagcg
     2041 cgcatcctcg aatccacctg cggcttctgc atcggcgcgg gccaggcccc caggaccgac
     2101 gctgtctcca tccggacgag caaccgaaac ttcgagggtc gcagcggcac caaaagcgca
     2161 aaggtcttcc tcgcgagtcc tgaatcggcc gtcgccgcag ccctgacagg agggatcacg
     2221 gatccccggg acctcggact cccctatccc gagatagaaa tgcccgggct gttctggatc
     2281 gatgacacga tgatcctctc cccggccaaa gaccccctgt ccg
//
LOCUS       DASSLD010000357         3260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_212885,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000357 DASSLD010000000
VERSION     DASSLD010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212885"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFK23_06425"
     CDS             <1..547
                     /locus_tag="VFK23_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7318754.1"
                     /translation="SIASLGPEIKNLTIVVNGVSKSHAMTGWRIGYAAGPKDVVSAMA
                     NIQSQSTSNPASISQKAAVEALRGPQDFIKTMNAEFDKRRRYMVDRLCAMTGVSCLMP
                     VGAFYAFPRVSSLYGKSANGKVIGNSSDLAAYLLEDARVALVSGDAFGADAYIRLSYA
                     TSLESITKGLDRIEAALQKIK"
     gene            652..1410
                     /locus_tag="VFK23_06430"
     CDS             652..1410
                     /locus_tag="VFK23_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318755.1"
                     /translation="MRIVTYILVVLLVATLGAAAFFYVNMFKPMAAEYPKMQAGMQEL
                     DKTKAELKRFKDKESRETSWLRPAAVALSAGLADEIKAGKAEVVIADHSVVVNLAEDT
                     IFMPESHTFTRESEKLLAKLESLLRSGELRGRQIAIGNTTEAVPAQGKGRKKIPAKDA
                     RTLAAERSAELVRYLEKKGTDQNALIAAAYSSKQAEAGSALRARKTVIIITSPLTVQA
                     VSPAAAPAANPSAPLEPQTAPKTIPIQPVKPKTN"
     gene            complement(1531..2343)
                     /locus_tag="VFK23_06435"
     CDS             complement(1531..2343)
                     /locus_tag="VFK23_06435"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7318756.1"
                     /translation="MAKAKRTAKPAAAAPQVESRNGSLIFAVALFLLIIGVLAAAAYA
                     RNSIYRTHVSLWENVTTRSPNKRRAHENYGQALSTAGSVSPDPAAAKKYYYEALRQFQ
                     MVMNLKDDGSVPLRDLYREVGVVYFRLGQFDEAISSWETGLRHAPNDPSLLNNLSVAF
                     LQKGRIEDAAKAAQTALTVDPYMPQALNTLGQAYMAKGDIEKSVQYFLKALEREPDVP
                     ARYWNVAIALDQAKKFDAALKYANAYASMERDPAGRQRAAQFLEHLKKMTGK"
     gene            complement(2496..>3260)
                     /locus_tag="VFK23_06440"
     CDS             complement(2496..>3260)
                     /locus_tag="VFK23_06440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HET7318757.1"
                     /translation="DLDVEDYLQGVVASEIPHDWKYEALKAQAVAARTYALYQKRTAG
                     RKAYHIVATVASQVYIGRRGERPRAAQAVRDTTGLVIEYNGELIPAFYHSSCGGHTEN
                     AYELWGIDAPYLRGVDCDCQEISRYGLWEKRIGKKQVIGAMRRLGYAASDILGMDIKG
                     ITPAGRVREVVVSTPRGKTYVPAESLRAALGNSIIPSVFYELAVEGDEAVFSGRGMGH
                     GVGLCQWGAEEMAEKGYGFEAILSHYYPGTGLMRLP"
BASE COUNT          659 a         1059 c          919 g          623 t
ORIGIN      
        1 gagcatcgca tcgctgggcc ccgagataaa aaacctcacg atcgtcgtca acggcgtttc
       61 aaagtcccac gccatgaccg gctggcgcat cggttatgcg gcaggcccca aggacgtcgt
      121 ctccgccatg gcgaatatcc agagccagag cacctcgaac cccgcttcca tctcccagaa
      181 agcggcagtc gaggcgcttc gcggtcccca ggatttcatc aagaccatga acgcagagtt
      241 cgacaaaaga aggagataca tggtggaccg cctatgcgcc atgacggggg tgtcctgcct
      301 tatgccggtc ggggcatttt atgccttccc ccgcgtctcg tccctttacg gcaagagcgc
      361 gaacggaaag gtcatcggaa actcctccga cctcgccgcc tatctgctcg aagacgcccg
      421 ggtagctctc gtatcgggcg atgccttcgg cgccgacgcc tacatccggc tctcctacgc
      481 gacgtctctg gagagcatca cgaaggggct cgaccggata gaggctgcgc tgcaaaaaat
      541 caagtagacg caatacggga gacggcagac agggctgaag acccagcatc ccgccgctct
      601 ccctcctgtc acctggctac cgtcttcttt caataaaggg ggtttctggc catgagaatc
      661 gtcacctaca ttcttgttgt actgctcgtc gctacgctcg gcgccgccgc cttcttctat
      721 gtcaacatgt tcaagccgat ggcggctgag tatccgaaaa tgcaggccgg aatgcaggaa
      781 ctcgacaaga cgaaggcgga attgaagagg ttcaaggaca aggaaagcag ggagacgtcc
      841 tggctgagac cggccgccgt cgcgctgagc gcggggctcg ccgacgaaat caaggccggc
      901 aaggccgagg tggtgatcgc ggaccacagc gttgtcgtga acctcgccga ggacacgatc
      961 ttcatgccgg agtcccacac cttcacccgg gagagcgaga agctcctcgc gaagctcgaa
     1021 tcgctcttga gatccggcga gttgaggggc aggcaaatcg cgatcggcaa cacaaccgag
     1081 gccgttcccg cccagggcaa aggcagaaaa aagatcccgg caaaggacgc gagaacgctc
     1141 gcggccgagc ggtctgcgga gctggtgagg tatctggaaa aaaagggtac ggatcagaat
     1201 gcgcttatcg ccgcggccta ctcgtcgaaa caggcggagg ccgggtccgc gctgagggcc
     1261 cgcaagaccg tcatcattat aacgagccct ctgacggttc aggccgtttc tcctgcggct
     1321 gccccggcgg cgaacccgtc cgcgcccctt gagccgcaga cagcgcccaa gacgatcccc
     1381 atccagcccg tgaagccgaa gacaaactag ggaaacgacg gcggacggct gcggaccgga
     1441 tgcgggtgtt tagaggcagg atcggttcag ggttctcagt ccgcactctc ctccgaatta
     1501 tgaaccatga actttgaagg taatctcaca tcacttcccc gtcatctttt tcaagtgctc
     1561 caggaactgg gctgccctct gacgccccgc agggtcccgt tccattgacg cgtacgcgtt
     1621 cgcgtacttg agcgccgcgt caaacttctt cgcctggtcc agggcgatgg cgacgttcca
     1681 ataccgggcg ggcacgtccg gctcacgctc cagggccttc aaaaaatact ggaccgactt
     1741 ttcgatgtcc cccttcgcca tgtacgcctg ccccagggtg ttcagggcct gcggcatgta
     1801 cgggtcgacc gtgagcgcgg tctgagccgc cttggccgcg tcttcgatcc gccccttttg
     1861 caggaatgcc accgacaggt tgttgagcag gctcggatca ttgggcgcat gacgcagtcc
     1921 cgtctcccag gaggagatcg cctcgtcaaa ctgccccagg cggaaataga ccactcccac
     1981 ctcacggtaa agatcacgca gcggaacgct cccgtcgtcc ttcaggttca tgaccatctg
     2041 gaactgacgc agcgcctcgt agtaatattt ctttgcagcg gcgggatcgg gggaaaccga
     2101 accggcggtg gacagggcct ggccgtaatt ctcgtgcgcc cggcgtttgt tcggagacct
     2161 cgtcgtgacg ttctcccaca acgacacatg cgtacgatag atgctgttcc tcgcataggc
     2221 cgcggcggca agaaccccga tgatcagcag aaacagggcc accgcaaaga tcagtgatcc
     2281 gttcctgctc tcgacctgag gtgcagcagc cgccggcttc gccgttcttt ttgcttttgc
     2341 cattgagact cccagccccc gggtcttgac gctgaccggg gcgcgaatgc gcttcggaag
     2401 ttccgccgta atgcggcctt ccggaaaaac attgtaaagt gagggatgat tcgagtcaag
     2461 ggaaatgatg cccgtggatc cacgcccgac tgcggtcacg gcaaccgcat caggcccgtc
     2521 cccgggtaat aatgcgataa aatcgcttca aaaccatacc ccttttcagc catttcctcc
     2581 gctccccact ggcagaggcc gacgccgtgc cccatccccc ttcctgaaaa aaccgcttcg
     2641 tccccctcca cggccagctc ataaaacacg ctgggaatta tcgagttccc cagcgccgct
     2701 ctgagcgact ccgctggaac atatgttttt cccctcggcg tcgaaacaac gacctcccgc
     2761 acccgtcccg cgggagtgat ccccttgatg tccatgccca ggatatcgct cgccgcgtac
     2821 cccagccgcc gcatggcgcc gatgacctgt ttcttcccga ttcttttttc ccaaagcccg
     2881 taccgggaga tctcctggca atcgcagtcg acgcccctga ggtacggcgc atcgatgccc
     2941 cagagttcgt atgcgttttc cgtgtgcccg ccgcagctcg agtggtagaa ggcgggaata
     3001 agctctccgt tatactcgat gacgagccct gtcgtgtccc gaaccgcttg cgccgcccgc
     3061 ggccgttcac ccctgcggcc gatgtaaacc tggctggcaa cggtcgcaac tatatgatac
     3121 gccttgcggc ccgccgttct cttttgatac agggcatagg tcctcgccgc cacggcctgc
     3181 gccttcagcg cctcgtattt ccagtcgtgc gggatttcgg acgccacgac cccctggaga
     3241 tagtcttcca catccaggtc
//
LOCUS       DASSLD010000358         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_293865,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000358 DASSLD010000000
VERSION     DASSLD010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293865"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..872)
                     /locus_tag="VFK23_06445"
     CDS             complement(102..872)
                     /locus_tag="VFK23_06445"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HET7318758.1"
                     /translation="MKKNYLVMLLSLMLALPLAANAFPPKKGDKDCIECHKLDKKDAE
                     AIVKKVVPTGTVTDIKQSPIKGVWQIDVDAGEGKRGALYLDFSKKNLVAGQIVPVESL
                     GKQQPPKKVDTSKIPLGDAIIVGPKDGAKKVIVFTDPDCPYCRELHKIMKQVIEKRHD
                     IAFELILNPLPMHKEAYKKAQAIQCSKSLEMLDDGFSGKTVPEPPAGCTADVIEKNKA
                     LAKTLEFSGTPTLVRDDGTVLFGYLPEEKLLEWIDKKQ"
     gene            complement(988..1800)
                     /gene="dapB"
                     /locus_tag="VFK23_06450"
     CDS             complement(988..1800)
                     /gene="dapB"
                     /locus_tag="VFK23_06450"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET7318759.1"
                     /translation="MIKAVVTGAAGRMGSRIINVLSASEGIRLSGAIERKGHALVGQD
                     ACGPAGLPAGGTLTLITDDLVAALKAGDVLIDFTFPEISLANLRVCAELGKPAVIGST
                     GFSKEQLAEVDKLVQKIPCILSPNMSVGVNVCFKVLADIAKTLGNDFDVEIVESHHRM
                     KKDAPSGTAVRMGEVVAGALGRDYRQTAKYHREGITGERTKEEIGMQTIRGGDIVGEH
                     TVYFIGMGERIELTHRAHTRDMFARGAVRAAKWVVGKKPGRYDMQDVLGLNN"
     gene            complement(1955..>2102)
                     /locus_tag="VFK23_06455"
     CDS             complement(1955..>2102)
                     /locus_tag="VFK23_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET7318760.1"
                     /translation="FIETNPIPVKTSLALMGRIAEEFRLPLCPMADANKEKLKKALKD
                     YGLL"
BASE COUNT          374 a          659 c          584 g          485 t
ORIGIN      
        1 ggcccgaaaa aatcccctgc gagttccacg acctcgtcgc cgtccaggac gccgcacgct
       61 gtcctgtccc cggcattgaa ccgtaccagt ctcatccagc gttattgctt cttgtcgatc
      121 cactccagga gcttttcttc cggcaggtag ccgaagagca cggtgccgtc gtcacgcacg
      181 agagtcggcg tgcccgaaaa ctccagggtc ttggccaggg ccttgttctt ctcaatcacg
      241 tctgccgtgc accccgccgg aggctccggc accgtcttgc cggagaaacc gtcatcgagc
      301 atctcgagcg acttcgagca ctggatcgcc tgtgccttct tgtacgcctc cttgtgcatg
      361 gggaggggat tgaggataag ctcaaaggcg atatcatgcc gcttctcgat cacctgtttc
      421 atgatcttgt ggagctcccg gcaatagggg cagtcggggt cggtgaagac gatcaccttc
      481 ttcgcgccgt ccttggggcc cacgatgatg gcgtcgccca acgggatctt cgacgtatcg
      541 accttcttgg gaggttgctg tttgcccagg ctttcgaccg gcacgatctg cccggcgacg
      601 aggttcttct tcgagaagtc aagatagagc gcgccacgct tgccttcgcc ggcgtccaca
      661 tcgatctgcc acacgccctt gatcggagac tgctttatgt ccgttaccgt tcccgtgggc
      721 acgaccttct ttacgatggc ctcggcgtcc ttcttgtcca gcttgtggca ctcgatgcag
      781 tccttgtcgc cctttttcgg agggaaggca ttcgccgcga gcggcagggc cagcatgagg
      841 ctgagcaaca tcaccagata attctttttc accgtagtct cctttttttg aagcttgaag
      901 gtgtgaaggt gggaagggaa gaaggtaaga aaagaaatgc ccatcgcagt tcctcgccta
      961 ccttcccacc ctcgtacctt cctactttca gttattcaac cccagcacgt cctgcatgtc
     1021 gtagcggccg ggcttcttgc cgacgaccca ctttgccgcg cgtacggcgc ctcgggcgaa
     1081 catgtcacgg gtatgcgccc gatgagtgag ctcgatccgc tctcccattc cgatgaaata
     1141 taccgtgtgt tctcccacaa tatccccgcc gcgtatggtc tgcatgccga tctcttcttt
     1201 cgtccgctcc ccggttattc cctcgcgatg gtatttggcc gtctggcggt agtcgcgccc
     1261 gagggctccg gccacaactt ctcccattcg cacggcggtg ccggatggcg cgtccttctt
     1321 catccggtgg tgcgactcga cgatctccac gtcaaagtca tttcccagcg tttttgcgat
     1381 gtcagcgagg accttgaagc agacattcac cccgacgctc atgttcggcg aaaggataca
     1441 gggtatcttt tgaacgagct tgtcgacctc tgcgagttgt tccttggaaa aaccggtcga
     1501 tccaatgact gcgggtttgc cgagttcagc gcagaccctg agattcgcga ggctgatctc
     1561 cggaaacgta aaatcaataa gtacgtcccc tgccttgagc gccgcaacga gatcgtccgt
     1621 gatcagggtg agcgttcccc cggccgggag ccctgcgggg ccgcaggcgt cctggcctac
     1681 gagcgcgtgg cccttccgct cgatcgcccc tgaaagacgg atgccctcgg aggccgagag
     1741 cacgttgatg atgcgcgagc ccatcctacc ggcggcgccg gtcactacgg ctttaatcat
     1801 attggtctcc tatgcgaaca ctatcggcgc ccgttcatgt cacggctgcc tagacgaaag
     1861 ggcgaggggg cgagggggag acgtggagaa aaagcttaga atctcttgac ctccttcgcc
     1921 cactcgcccc gtcgccgatt cgccgtttca atccttacaa aagcccgtag tccttcagcg
     1981 ccttcttgag cttctccttg ttcgcgtcgg ccatgggaca gagcgggagc cggaactcct
     2041 cggctatcct gcccatgagc gcaagcgacg ttttgaccgg gatcgggttc gtttcgatga
     2101 ac
//
LOCUS       DASSLD010000359         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_727735,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000359 DASSLD010000000
VERSION     DASSLD010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727735"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VFK23_06460"
     CDS             <1..810
                     /locus_tag="VFK23_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014322012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7318761.1"
                     /translation="TPLLIMFGIPSTVAAATDSNQIVAASTSGTYAHWKVGNVDFKMG
                     LYLLVGGFLGGLVGVQLIKLLHAMGNADFVIKMTYVLMLGIVGVYMFIESLQSMKKNK
                     SAAFEAKKEEKESAVGKFLKSLPFQTRFEKSGITHSVLVPIVLGGFVGILAAIMGVGG
                     GFLMVPIMVYMLRMPMHVVVGTSLFQILFTCIEVTFLQAYTNHSVDFVLAVLLLLGST
                     IGAQVGAVFGRKLKADQLKILLAGIVLAVTVKIVFELTLAPSLLISQAGGH"
     gene            825..1613
                     /locus_tag="VFK23_06465"
     CDS             825..1613
                     /locus_tag="VFK23_06465"
                     /inference="COORDINATES: protein motif:HMM:NF021148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HET7318762.1"
                     /translation="MRNAKCGMMATAALALIITFGFAGTASAMLTAKANHDHITIDFF
                     YHGSTVSVKGESDPGVDLIIKMTAPEEHQTLKQKGKVGGLLWMNVGTLKFEHVPNFYA
                     VYSTKKTEDILSREELEKYTIGYPALERHVEVTPVANEEEKAKWFGEFVKFKEDARVY
                     AMSSGKIETKTTDTGRQGYYILTDWPYQAAPGDYLITVYAVKDGKVVEQAETKVNVEQ
                     VGVVKTLATMAKNSAAFYGILSIGIALGAGFGVGLVFRKGGGSH"
     gene            1613..2476
                     /locus_tag="VFK23_06470"
     CDS             1613..2476
                     /locus_tag="VFK23_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7318763.1"
                     /translation="MYSAIVVGYDESESSKAALKEAAFRVKNHGGKLYLVHAVYFDKE
                     EFAILPSQMEQRFEIGSQVCRLAKNDVQSEFGLNGSVESLVCEGEPPQVLVETAEANK
                     ADLIALGTYGRKGLKRLLMGSVTSQVILTAPCDVLVVKKPCSKCSGRYHSLLVPFDGS
                     ESSKRALTRACELSKADGAEVSVLYVIPRYEEMMDFFKTEAIKKSLYREAEKIVGAAK
                     NLAAALGVQIKAVVQDGRAGDKIVEIADTMKNDLIVVGTHGWSGMNKAIMGSTAERVI
                     ATSSCPILIAK"
     gene            2494..2916
                     /locus_tag="VFK23_06475"
     CDS             2494..2916
                     /locus_tag="VFK23_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7318764.1"
                     /translation="MKGYRKVLIAVNGSKEVLKQGIRLAQDEKTWITVVKVVQPYEGD
                     LNLTGIKNLNDVLDDGGARAIRDINRIAEAEGALVKTRLEAGDVPDRIVEVAEEEQCD
                     LIIMGSKKKKGILSRLFGDHVVEKVIDRASCPVYVVGL"
BASE COUNT          722 a          799 c          945 g          575 t
ORIGIN      
        1 acgccgctcc tcatcatgtt cggcatcccc tcgaccgtgg cggcagcgac agattcgaac
       61 cagatcgtgg ctgcctcgac gtccggcacc tacgcgcact ggaaagtcgg caatgttgat
      121 ttcaagatgg ggctgtacct cctggtcggc ggttttttgg gcggtctcgt cggcgtccag
      181 ttgatcaaac tgcttcatgc catgggtaac gccgacttcg tgattaaaat gacctacgta
      241 ctcatgctcg gcatcgtggg cgtttacatg ttcatcgaaa gcctccagag catgaagaaa
      301 aacaagtcgg cggcttttga ggccaaaaaa gaggaaaagg agtcagcggt cggaaagttc
      361 ctcaagtcat tgccgttcca gacccgtttc gagaaatccg gcatcaccca ttcggtcctg
      421 gtgccgatcg tcctgggcgg gtttgtcggc atccttgcag ccatcatggg tgtgggcggc
      481 gggttcctga tggtgccgat catggtctac atgctccgga tgcccatgca cgtcgtggtc
      541 ggcacgagcc tgttccagat cctgttcacc tgcatcgagg tgacgttcct gcaggcctat
      601 acgaaccaca gcgtcgattt tgtgcttgca gttcttctcc tgctgggatc gaccatcggc
      661 gcccaggtcg gagcggtatt cggccggaag ctcaaggcag accagctcaa gatcctgctt
      721 gccggcatcg ttctggccgt gacggtcaag atcgttttcg aactgacgct tgcgccttca
      781 ttacttatat ctcaggcagg aggacattaa taatgaattg cggaatgcgg aatgcgaaat
      841 gcggcatgat ggcaacagcg gcacttgctt tgatcataac gttcgggttt gccggcacgg
      901 catctgccat gctcactgcg aaagcgaacc atgaccatat caccatcgac ttcttctatc
      961 acggaagcac cgtgagcgta aagggagagt cggaccccgg cgtggacctc atcatcaaga
     1021 tgacggcccc cgaggaacac cagaccctga agcagaaggg aaaggtcggg gggcttcttt
     1081 ggatgaacgt gggaaccttg aaattcgagc acgtgccgaa cttctacgcc gtctatagca
     1141 cgaaaaagac ggaagacatc ctgagccgag aagagctgga aaaatatacg atcggatacc
     1201 ccgcgctgga gaggcatgtt gaggtgaccc cggtcgccaa tgaggaagag aaagccaaat
     1261 ggttcggtga gtttgtcaaa ttcaaggagg acgcccgggt ctacgccatg tcatcgggca
     1321 agatcgagac gaaaacgacc gacaccgggc ggcagggata ttatatcctg accgactggc
     1381 cgtaccaggc cgcgcccggc gactacctga tcacggtcta cgccgtcaag gacggcaagg
     1441 tcgtggagca ggctgaaacc aaggtgaatg ttgaacaggt cggcgtggta aagactctgg
     1501 cgaccatggc gaaaaacagc gcagccttct acggaatact ctccatcggg atcgccctgg
     1561 gagcgggctt cggcgtgggc ctggtgttca ggaagggcgg aggttctcat taatgtacag
     1621 cgcaattgtc gtcggatacg acgaatcgga atcgagcaag gctgcgctca aggaagccgc
     1681 gttccgggta aaaaatcacg gtgggaagct ttacctggtc catgcggtct acttcgacaa
     1741 ggaggagttc gccatcctgc cgtcgcagat ggaacagcgg ttcgagatcg ggtcgcaggt
     1801 atgccgcctg gcgaagaacg acgtgcagtc cgagttcggc ctgaacggga gcgtggaatc
     1861 cctcgtatgc gagggcgagc ctccccaggt gctcgtcgag acggccgagg caaacaaggc
     1921 ggacctcata gccctgggga cctacggcag aaaaggactg aagcggctgc tgatgggcag
     1981 cgtgacgtcc caggtcatcc tcaccgcccc ctgcgacgtg ctcgtggtca agaaaccgtg
     2041 ctcgaaatgc agcgggcgct atcattcgct cctggtcccc ttcgacgggt cggagtcgag
     2101 taaaagggcg ctgacgcggg catgcgagct gtcgaaggcg gacggcgccg aggtgtcggt
     2161 tctgtacgtg ataccccgct acgaagagat gatggacttc ttcaagaccg aagccatcaa
     2221 gaagagcctc taccgggaag cggagaagat cgtcggcgcg gcgaagaatc tcgctgcggc
     2281 gctcggggtg cagatcaagg ctgtcgtgca ggacggccgc gcaggcgaca agatcgtgga
     2341 gatcgccgat acgatgaaga acgatctcat cgtcgtgggg acccacgggt ggagcggcat
     2401 gaacaaggcg atcatgggga gcacggcgga gcgggtcatc gctacttcct catgtcctat
     2461 cctgatcgca aaataacgcg ggagatcaga gccatgaaag gctacagaaa agtcctcatc
     2521 gcagtgaacg gatcaaagga agtcctgaaa cagggaatca ggctggccca ggatgaaaag
     2581 acctggatca ccgtcgtgaa ggtggtccag ccctacgaag gggacctgaa cctgacgggc
     2641 atcaagaacc tgaacgatgt acttgacgac ggaggggccc gtgcgatccg cgacatcaac
     2701 aggatcgcgg aggctgaagg cgccctggtc aagacgcgtc tcgaagccgg cgacgtgccg
     2761 gacaggatcg tcgaggtcgc ggaagaggaa caatgcgatc tcatcatcat gggctcgaag
     2821 aagaaaaaag gcatcctctc ccggctcttc ggcgatcatg tcgtcgagaa ggtcatcgac
     2881 cgggcgagct gcccggtata cgtggtaggt ctgtaattcc gttttgcgaa catgattaaa
     2941 aggtgtacga taaccaacgt gaaggattat ttgtatgccc gccgatagag gaaagaaatg
     3001 aaggcataga acgcacggac gaaaggttca gagaaacagg g
//
LOCUS       DASSLD010000360         3030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_404660,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000360 DASSLD010000000
VERSION     DASSLD010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404660"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VFK23_06480"
     CDS             <1..658
                     /locus_tag="VFK23_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET7318765.1"
                     /translation="TKIAVASKESQVDPVGACVGMKGSRVQAVVRELRGEKIDIIPWS
                     EDPRIFIAKALSPAVVEKVGVTEEDRSALAVVADSQLSLAIGKKGQNVRLAAKLTGWK
                     IDILSESEYDQERQKEREQEIEAAIVEETRKMAEETEAAQTMETQTSGDSAPAAPADG
                     LTAIEGVGEKTAEALREAGYDTVEKIAAMSDEEILAVPGIGAKTAQKILLSARALLGK
                     "
     gene            813..1394
                     /locus_tag="VFK23_06485"
     CDS             813..1394
                     /locus_tag="VFK23_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HET7318766.1"
                     /translation="MKHQPERTCIGCRGVFKKNEVVRIVAGPEGVLIDYREKLPGRAA
                     YVCPRKECIDKALSKENLSKALHLKVRTPDAAAFITRLEDAVREKIRSLLSMAMKAGK
                     TATGYSAVQDALDKGRVELLLYAADLSDGTRGKLAFGDAKEVRHATLFNRDEFGRLTG
                     RELVGVVGILDKGFAETLWYETQRLKGLINIRD"
     gene            1444..>3030
                     /locus_tag="VFK23_06490"
     CDS             1444..>3030
                     /locus_tag="VFK23_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HET7318767.1"
                     /translation="MTVIRVHELAKKMGVESKDLISVLDKIGIKGKTPSSGLDDKESK
                     SLIEKLKALRKEKEKIKKEKETVDPLLSKEERLKKAAALIGKFSSTLEKPGAKPKSAP
                     IPPLPKPAMPAPPVIPPASQLKAAQAAALEEKKKAAEAAPAPQPASTAERPQPAPGSV
                     EQVVRPVTSARPGPAARPQPGQRPGGGQGRPSGRPFAKSTFGSPVLEASQVPASLAPP
                     APGKPAKKKWDKKKSEYDQKQEREFREKPSFKKLPDLKTLPLGKRPLKKDDRHGQQTD
                     VAEITKPRKKVVKIQEGCTVKEFAEVMGQKVGDVIKKLMAMGIMATQNQPMDVDASLL
                     LADEYGVKAEIASIESAEDILEEKADAAETMVPRPPVVTIMGHVDHGKTSLLDAIRET
                     NVVSREAGGITQHIGAYQVSLKGRDISFLDTPGHAAFTAMRARGAQVTDIVILVVAAD
                     DGVMPQTKEAISHAKAAGVPIVVAINKIDKPEAKPDRVKQELTEFELIPEEWGGQTIF
                     CEVSAKKKIGLEHLLEMVLIQ"
BASE COUNT          779 a          840 c          942 g          469 t
ORIGIN      
        1 caccaagatc gccgtcgcct ccaaggaatc ccaggtcgac cctgtcggcg cgtgcgtggg
       61 catgaagggt tcccgcgttc aggctgtggt gcgcgaactt cggggcgaga agatcgacat
      121 cataccctgg tccgaagacc cccgtatctt catcgcgaaa gccctgagcc cggctgtcgt
      181 ggaaaaggtg ggggtgaccg aggaggaccg gtcggcgctt gccgtggtcg ccgattcgca
      241 gctttccctt gccatcggca aaaagggcca gaacgtgagg cttgccgcga aacttacagg
      301 ctggaagata gacatcctga gcgaatccga gtacgatcag gaacggcaga aagagcgcga
      361 gcaggagatt gaggcagcca tcgtcgagga aacgcgcaag atggcggagg agacggaagc
      421 agcccagacc atggagacac agacttccgg ggactcggcg ccggcggcgc cggcagacgg
      481 cctgacggca atcgaaggcg tgggtgagaa gaccgcggaa gcgctcaggg aagcgggcta
      541 cgacacggtc gagaaaatcg cggccatgtc cgacgaggag attcttgccg taccggggat
      601 aggagcaaaa acagcccaga agatccttct atcggccagg gcgctgctgg ggaagtaatt
      661 gcccttaaag aatttcaagc tgtcatacct gcgaaagcag gtatccattg tcttctatca
      721 aataaacttc aaaatggatt cccggtcccc gatagaatca ttcgaggaca agtttcgcag
      781 cgggaatgac aacatgggta ccaacgaaaa caatgaaaca tcaacccgaa aggacctgca
      841 tcggctgcag aggtgtgttt aagaaaaacg aggtcgtgag gatcgtcgcc ggaccggagg
      901 gggtcctgat cgactaccgg gaaaagctgc ccggccgggc ggcctatgtc tgtccgagga
      961 aggagtgcat cgacaaggca ctctcgaagg aaaacctctc gaaggcgctt catctgaagg
     1021 taaggactcc ggacgcggct gcgttcatca cgcgtctcga agacgcagtc agggagaaga
     1081 tccgatcgct gctttccatg gcgatgaagg cgggcaagac cgcaacgggg tattcggcgg
     1141 tgcaggacgc ccttgataaa gggagggtgg agcttctgtt gtatgccgcc gatctttcag
     1201 acgggactcg cggtaaactg gcgtttggag acgcaaaaga ggtgcgtcac gcaacgctgt
     1261 tcaaccggga cgagtttggc cgcctcaccg gcagggaact cgtcggagta gtcggaattc
     1321 tcgacaaagg attcgcagaa acgctctggt acgagacgca aagattaaaa ggcttgataa
     1381 atatcaggga ctagagtaca ataacatgat tcataagaag attttggggg ggttagcgat
     1441 tttatgaccg taatcagggt gcatgagctg gcgaaaaaaa tgggggttga gagcaaggac
     1501 ctcatttcgg tgctcgacaa gatcggcatc aagggaaaga ctccgtccag cggtctcgac
     1561 gacaaagaat cgaagtccct gatcgaaaaa ctcaaggctc tacgcaaaga aaaggaaaag
     1621 atcaagaagg aaaaagagac ggtcgacccc ttgttgtcga aagaggagcg gctgaaaaag
     1681 gcagccgcgc tgatcggaaa attttcgtcc accctggaaa aacccggcgc aaagccgaaa
     1741 tcggcaccca ttccgccgct accgaagccg gccatgccgg cgccgccggt cattccgccc
     1801 gcaagccagt tgaaggcagc ccaggcagcg gcgctggaag agaaaaagaa ggcggcggaa
     1861 gcggccccgg cgcctcagcc cgcttcgacc gcagagcgac cgcagcctgc gccgggcagc
     1921 gtcgagcagg tggtccgtcc tgttacatcg gcgcgaccag gaccggcagc ccggccccag
     1981 cccggacagc ggcccggggg tggtcaggga cggccgtcgg ggcggccctt cgcaaaatca
     2041 acctttggct cccccgttct ggaggcatcc caggtgccgg catccctcgc tcctcccgct
     2101 cccggcaagc cggcaaagaa aaaatgggac aaaaagaaat ccgaatacga ccagaagcag
     2161 gagcgcgagt tccgcgagaa accttccttc aagaagctcc ccgacctgaa aacgctcccg
     2221 ctcgggaaga ggccgctcaa aaaagacgac cgtcacggcc agcagaccga cgttgcggag
     2281 atcaccaagc cgagaaaaaa ggtcgtcaag atacaggaag gctgcacggt caaggaattc
     2341 gccgaggtca tggggcaaaa agttggcgac gtcatcaaga aactcatggc catgggcatc
     2401 atggcgacgc aaaaccaacc catggacgtg gacgcgtcgc tactccttgc cgatgaatac
     2461 ggtgtcaagg ccgagatcgc gagtatcgaa tcagccgaag acatcctcga ggaaaaggct
     2521 gatgccgccg agaccatggt gccgcggccc ccggtggtca ccatcatggg ccatgtggac
     2581 cacggaaaga cgtcgctcct cgatgccatc cgcgagacca acgtcgtatc acgggaagcg
     2641 ggtgggatca cccagcatat cggcgcgtac caggtcagcc tcaaaggcag ggacatttcg
     2701 ttcctcgaca caccgggcca tgcggcgttc accgccatgc gcgcccgcgg agcccaggtt
     2761 acggacatcg tgatcctcgt ggtcgccgcc gacgacggcg tgatgccgca aaccaaggag
     2821 gctataagcc acgctaaagc ggcgggggtg ccgatcgtcg tcgcaatcaa caaaatcgac
     2881 aagcccgagg ccaaacccga ccgggtgaag caggagctta ccgaattcga actcatccct
     2941 gaagagtggg gcggccagac gatcttctgc gaggtctcgg caaagaaaaa gattggactc
     3001 gagcacctgc tcgagatggt gctcatacag
//
LOCUS       DASSLD010000361         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_783528,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000361 DASSLD010000000
VERSION     DASSLD010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783528"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFK23_06495"
     CDS             <1..345
                     /locus_tag="VFK23_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET7318768.1"
                     /translation="HRQGNEEDWRKFKVKYQGKEYCKDCHADKYTSINKTPHRIIQCE
                     NCHGPAIDHPSDPPKLTIDKSRAQCLRCHYPLPYPTSGRKNIRGIDPAVHNPDVECAT
                     CHNPHNPMGGLR"
     gene            342..1133
                     /locus_tag="VFK23_06500"
     CDS             342..1133
                     /locus_tag="VFK23_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7318769.1"
                     /translation="MITRRNFLKKTAMGIAGATLPLAALKIIDPKKLLAEAAADKSSA
                     VRWVFLVDTYKCVGCGLCVKACKLENEIPYDANVTRTWVERYVLTKQGELLADTPKGA
                     RDGFTTTKIDLGRGEYKNVKNQDIEKAFFVPKLCNQCENPPCVQVCPVGATYQAPDGV
                     VLVDRTWCIGCGYCIMGCPYGVRFFHPVYHTAEKCNFCYHRITKGLKTACVDACPFGA
                     RQIGNIKDPNDPVTKVIRDQRVGVLKSEFGTKPQVYYIGLDEEVR"
     gene            1133..>2040
                     /gene="nrfD"
                     /locus_tag="VFK23_06505"
     CDS             1133..>2040
                     /gene="nrfD"
                     /locus_tag="VFK23_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET7318770.1"
                     /translation="MVHGELWTVKELFVLPNEYIYWSIQIVMYPFMTGLVAGAFVLSS
                     LYHVFGIKELKDMARFALVFSFALLFAAPMPIVLHLQFPFRGINVFMTPHFTSAIAAF
                     GIVFFTYGAIVASELWFLYRKHFVEVSLALREKRDKTLGEWIKYLIFCTLTLGVYDVS
                     EEALHRDEKAIKFLAGLGIPVACFLHGYAGFIFGSVKANALWMTPLMPVIFIMSAIVS
                     GIALCMLTYIVTMEIRRMLAARKRTQPGRRYESMEEIKGVEIHVIKMTSKYLLSFLVA
                     AISLEILDLIFRGYTALKSWDVLRIV"
BASE COUNT          457 a          569 c          571 g          443 t
ORIGIN      
        1 catcggcagg gcaatgagga agactggagg aagttcaagg taaaatatca gggcaaggaa
       61 tactgcaagg attgccacgc cgacaagtac accagtatca ataagacccc gcacaggatc
      121 atccagtgcg agaactgcca cggtcccgcc atcgatcatc cgtcggaccc cccgaagctg
      181 accattgaca agagccgggc gcagtgcctc cggtgtcatt acccgctccc gtatccgacg
      241 agcggacgaa aaaatatcag gggcatcgat ccggccgttc ataatcccga cgtggagtgt
      301 gccacctgtc acaacccgca taatccgatg ggaggcttaa gatgattacg cgaaggaact
      361 ttctgaaaaa gacggctatg gggatcgccg gcgcgacgct ccccttggcg gccctgaaga
      421 tcattgatcc caaaaaactg ctcgctgaag ccgcggcgga taaatcctcc gccgtacgat
      481 gggtgtttct cgttgacacc tacaaatgcg tgggctgcgg cctgtgcgtg aaggcctgca
      541 agctcgagaa tgaaattcct tatgacgcca atgtgacgag gacatgggtc gagcgatatg
      601 tcctgacgaa gcaaggggaa ttgctggccg atacgccgaa gggagcgcgc gatggtttca
      661 cgaccaccaa aatcgatctc gggcgtgggg agtacaagaa cgtaaaaaac caggacattg
      721 aaaaagcgtt cttcgttccg aaactctgca accagtgcga aaacccaccc tgcgtgcagg
      781 tgtgccccgt aggagcgact taccaggcgc cggatggggt cgtgcttgtg gaccggacat
      841 ggtgcatcgg atgcggctac tgcatcatgg ggtgtcccta cggcgtgcgg ttcttccatc
      901 ccgtatacca tacggcggag aagtgcaatt tctgctatca tcggatcacg aaaggcctga
      961 agacagcctg tgtagacgcc tgccccttcg gcgcgcggca gatcgggaac attaaagacc
     1021 ccaatgaccc ggtgacgaag gtcatcaggg accagcgagt aggggttctc aagagcgaat
     1081 ttgggacgaa gccacaggtc tattacatcg gcctggatga ggaggtgcgg taatggtgca
     1141 cggagaactt tggaccgtta aggaactgtt cgttttgccc aatgaatata tctactggag
     1201 cattcagatc gtgatgtacc cctttatgac ggggcttgtt gccggcgcct tcgtcctgtc
     1261 gtcgctttat catgttttcg gcatcaagga gctgaaggac atggcccggt tcgccctcgt
     1321 gttctccttc gccctccttt tcgcggcgcc tatgccgatt gtcctccatc tgcagtttcc
     1381 cttccggggt atcaacgtgt tcatgacgcc ccacttcacc tcggccattg ccgccttcgg
     1441 catcgtattc ttcacctatg gcgctatcgt cgcatcggag ctctggttcc tataccggaa
     1501 gcacttcgtt gaagtatccc tggcgctccg ggagaagcgc gacaagacgc tgggcgaatg
     1561 gataaagtac cttattttct gcacccttac gctcggtgtg tacgacgtca gcgaggaggc
     1621 tctgcaccgg gacgagaagg cgatcaagtt ccttgctggc ctcggcatcc ccgtcgcatg
     1681 cttccttcac ggttatgccg ggttcatctt cggatcggtg aaggcgaacg cgctctggat
     1741 gacgccactc atgccggtca tcttcatcat gtccgcgatc gtgtcgggca tcgccctctg
     1801 catgcttacc tatatagtca ccatggaaat ccggagaatg ctcgcagcac gcaagaggac
     1861 gcaacctggc aggcggtacg agtccatgga agagatcaag ggtgtggaaa tccacgtcat
     1921 caaaatgacc tcgaaatatc ttctgtcctt cctggtggcc gctatcagcc tcgaaatcct
     1981 tgacctcatc ttccgcgggt atacggcgct aaagtcctgg gatgttctcc gaatcgtcat
//
LOCUS       DASSLD010000362         3558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1185329, whole genome shotgun sequence.
ACCESSION   DASSLD010000362 DASSLD010000000
VERSION     DASSLD010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185329"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFK23_06510"
     CDS             <1..317
                     /locus_tag="VFK23_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318771.1"
                     /translation="AKENETVIARGGKPATARPILLGITKASLTTDSFISAASFQETT
                     RVLTEAAITGAVDDLVGLKENVIMGRLIPAGTGMTEYRNTYVVVEDESEQTGQVVEES
                     HT"
     gene            492..863
                     /gene="rpsL"
                     /locus_tag="VFK23_06515"
     CDS             492..863
                     /gene="rpsL"
                     /locus_tag="VFK23_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET7318772.1"
                     /translation="MPTISQLVRKGRSLAASKTKSPALKSCPQKRGVCIRVYTTTPKK
                     PNSALRKVARVRLTNGMEVTTYIPGVGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGTL
                     DTVGVANRKQGRSKYGAKTPK"
     gene            941..1411
                     /gene="rpsG"
                     /locus_tag="VFK23_06520"
     CDS             941..1411
                     /gene="rpsG"
                     /locus_tag="VFK23_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET7318773.1"
                     /translation="MPRKTVIVKRETLPDPKYNSKMLAKFVNVLMRKGKKSTAEAICY
                     KALDRIQAKTNSDPIAVFKKAVENLKPALEVKSRRVGGASYQVPIEVKQPRRLSLAYR
                     WLRLYASQRPEKSMVDKLAGEILDASNNTGAAVKKKDDVHRMAEANKAFAHYRW"
     gene            1438..3531
                     /gene="fusA"
                     /locus_tag="VFK23_06525"
     CDS             1438..3531
                     /gene="fusA"
                     /locus_tag="VFK23_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET7318774.1"
                     /translation="MARQYSLENTRNIGIMAHIDAGKTTTTERILYYTGVSYKIGEVH
                     DGTAVMDWMVQEQERGITITSAATTCFWRDKRINIIDTPGHVDFTIEVERSLRVLDGA
                     VAAFDSVSGVEPQSETVWRQADKYQVPRIAFMNKMDRIGADFYMSVQSMFDRLGANAV
                     PIQLPVGAEDKFRGCVDLVKMKSVLFNDETLGAKYVVEDIQADLLPKAKEYREKMIEK
                     LADVDDVIMEKFLNGEQPTEAEIMAAIRKGTIAMKIFPVICGSAFKNKGVQLLLDAVV
                     DYLPSPLDIPPVTGIDPKTSAEIVRKTSDSEPFSAVAFKIMTDPFVGQLTFIRVYSGT
                     LNSGSYVYNSTKGTRERIGRLLKMHANKREEIQQIYSGDICAAVGLKSTLTGHTLCDE
                     NKPIILEAMTFPEPVISVAIEPKTKADQEKLGLSLQKLMQEDPSFRVFTDQETGQTII
                     SGMGELHLEIIVDRLMREFKVDANVGKPQVAYRETIKKKVTAEGKFIRQSGGRGQYGH
                     VVLQLEPQEPGKGFEFVNKTVGGSVPKEYVAPTQKGIEEAMENGVLAGYPVVDMKVTL
                     IDGSYHDVDSSEMAFKIAGSMGFKEGAAKASPVLLEPIMAVEVVCAEEYMGDIIGDLN
                     SRRGRVQGMNVRGAGRVITAQVPLSEMFGYATDIRSKTQGRATYTMQFAHYEEVPKTI
                     SEQIIAKVKGETVKK"
BASE COUNT          912 a          945 c         1049 g          652 t
ORIGIN      
        1 ttgcgaagga gaacgagacc gtgatcgccc gtggcggcaa gccggctacg gcacggccca
       61 ttctgcttgg aatcaccaag gcttcgctca cgacggacag cttcatttcc gcggcgtcgt
      121 tccaggaaac cacgcgggtg cttacggagg ccgccatcac cggcgccgtg gacgatctgg
      181 tcggactgaa ggaaaacgtg atcatgggcc gtctgattcc tgcgggtacc ggcatgacgg
      241 aataccggaa cacctatgtc gtcgtcgagg acgagagcga gcagactgga caggttgtcg
      301 aggagagtca tacctagcat tcccggcacg ggcctggaga tgtcctgtaa gtgcgttatc
      361 tatttattaa ggtaataact aaaacgggat aaaaaaagct tgacaactcc ggaccatatc
      421 gatataatta ccgggttttg actcaatgtt tttaaaaaag ttgtatttcg ttgtttttaa
      481 ggagtaatct cgtgcctacc atcagccaat tggtaagaaa aggcagatca ctcgccgcaa
      541 gcaagaccaa gagccccgcg ctcaagagct gcccccagaa gcggggggtc tgcatcaggg
      601 tctacaccac gacaccgaag aagccgaact cagcccttcg aaaggtagcc agggtgaggc
      661 tcacgaacgg aatggaggtt acgacgtaca ttccgggcgt aggacacaac ctgcaagagc
      721 actccatcgt gctcatccgg ggaggaagag tcaaggacct cccgggcgta cgataccaca
      781 tcgtacgggg tacacttgat accgtgggag ttgcaaaccg caaacagggc cgctccaaat
      841 acggggcgaa gacgccaaaa taaggaaatc gagggatgaa ggggagacga aggacgcaaa
      901 atcgtccacc atccttcatg taatcttaag gaagacgcat atgccgagaa aaacagtcat
      961 cgtaaagaga gagacactgc cggacccgaa gtacaacagc aagatgctcg cgaagttcgt
     1021 gaacgtgctc atgcgcaagg gaaagaagag cacggcggaa gccatctgct acaaggcgtt
     1081 ggaccgcatc caggcgaaga ccaacagcga tcctatcgcc gtattcaaga aggccgtgga
     1141 gaacctgaag cctgcccttg aagtgaagtc ccgcagggtc ggcggcgcat cataccaggt
     1201 gccgatcgaa gtcaagcagc cgcgccggct ttcgctggcc taccggtggc tgcggctcta
     1261 cgcgtcccag cgtcccgaaa aatccatggt ggacaagctt gccggggaga tactcgatgc
     1321 atcgaacaat accggcgcgg cggtaaaaaa gaaggacgac gtgcaccgga tggcagaggc
     1381 caacaaagcc ttcgcccact atcgctggta aggcatacct gaggagcgga ggaagttttg
     1441 gctaggcagt attcactcga aaatacccgg aacatcggca tcatggcgca catcgacgcc
     1501 ggaaaaacga cgacgaccga gcgcattctg tattataccg gtgtttccta taagatcggc
     1561 gaagttcacg acggcacggc ggtcatggat tggatggtgc aggagcagga acgcggcatc
     1621 accatcacct cggccgcgac cacctgtttc tggcgggaca aacggatcaa tatcatcgac
     1681 actcccggcc acgtggactt taccattgaa gtggagcggt ccctccgcgt gcttgacggc
     1741 gcggtggcgg cgttcgactc tgtaagcggc gtcgaacccc agtcggagac ggtctggcgc
     1801 caggcagaca agtatcaagt cccacgcatc gcgttcatga ataagatgga ccgcatcggc
     1861 gcggacttct acatgagcgt gcagtcgatg ttcgaccggc ttggcgccaa cgcggtgccg
     1921 atccagctcc ccgttggcgc cgaggacaag ttccgcggct gcgtcgacct ggtgaagatg
     1981 aagtccgtgc tgttcaacga cgagaccctc ggcgcaaaat acgtcgtgga ggacatccag
     2041 gcggaccttc tgcccaaagc gaaagagtac cgcgagaaga tgatcgaaaa gcttgccgac
     2101 gtggacgacg tgatcatgga aaagttcctg aacggcgagc agcccacgga agccgagatc
     2161 atggccgcga tccgcaaagg cacgatcgcg atgaagatat ttccggtcat ctgcggatcg
     2221 gcattcaaga ataagggtgt gcagctgttg ctcgatgcgg ttgtggacta tctcccctca
     2281 ccgctggaca tcccgccggt aacgggaata gatcctaaga ccagtgcgga aattgttcgt
     2341 aagacttccg acagcgaacc cttttcggcc gtggccttca agatcatgac cgatcccttc
     2401 gtgggtcagc ttaccttcat acgggtttac tccgggacgc tcaattccgg gtcctatgtc
     2461 tacaactcga ctaaggggac gagggaacgt atcggaaggc tgctcaagat gcatgcaaac
     2521 aagcgcgagg agatccagca gatctactca ggagacatct gtgctgctgt cggactgaag
     2581 agcacgctga cgggtcatac gttgtgcgac gagaacaaac ccatcatcct tgaggcaatg
     2641 acgttcccgg agccggtcat ctccgtggcg atcgaaccga agacgaaagc ggaccaggaa
     2701 aagctcggct tgtcgctcca gaagttgatg caggaggacc cgtcatttcg tgtttttacc
     2761 gaccaggaga caggacagac catcatctcc ggtatgggcg aacttcatct cgagatcatc
     2821 gtcgatcgcc tgatgcgtga gttcaaggtg gatgcgaatg tcggcaagcc gcaggtggcg
     2881 tatcgcgaaa cgattaagaa gaaagttact gcagaaggca agtttattcg gcagtcaggc
     2941 ggacgcggtc aatatggcca tgtggtcctc caactcgagc cgcaggagcc gggaaaaggc
     3001 tttgaattcg tgaacaagac cgtcggcggc tcggttccca aggaatacgt cgcgcctact
     3061 cagaagggta tcgaggaggc catggaaaat ggcgtgctcg ccgggtatcc cgtggttgac
     3121 atgaaagtca ccctcatcga cggttcgtac cacgatgttg actcgtccga aatggccttc
     3181 aaaattgccg gttccatggg attcaaggaa ggcgcagcca aggccagtcc ggtgttgctc
     3241 gagccgatca tggccgtcga ggtagtatgc gccgaggagt acatggggga catcattggc
     3301 gatctcaact cccgccgcgg ccgggtacaa ggcatgaacg tacggggagc cggaagagtc
     3361 atcacggcgc aggtgccgct ctcggagatg ttcgggtatg cgacggacat caggtccaaa
     3421 acacagggca gggcaaccta caccatgcag tttgcccatt atgaagaagt cccgaaaacg
     3481 atatcagaac agattatcgc caaggtcaaa ggcgaaacgg taaagaaata atcaccgatc
     3541 cgaagaagca tctgtacg
//
LOCUS       DASSLD010000363         3795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1369609, whole genome shotgun sequence.
ACCESSION   DASSLD010000363 DASSLD010000000
VERSION     DASSLD010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369609"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..857
                     /locus_tag="VFK23_06530"
     CDS             51..857
                     /locus_tag="VFK23_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318775.1"
                     /translation="MKRYIAAVLAAIISSGCSGTVTKSFKVFADPPDADIRVVSGEDL
                     KELKYRSPAVVTAEVPKDPALASKAVVEVSKDSYKPFTLALRNIRDGQTLNVRLEKIV
                     QSMYRYRLAYRLVAPAISETLQFRDATIAVSFTVDGRSIQMRLTNLLDKNLKLLWEQA
                     QYTDVNKQTSRLMHSGVRFQDRNNPIPEQIVPPRASIQEGVIPINRVVFSQEKKAYET
                     KALLPIESESGAQLKGKTINLFIPIEVDRAIIPYNFKIEITDSVREDVKG"
     gene            989..1231
                     /locus_tag="VFK23_06535"
     CDS             989..1231
                     /locus_tag="VFK23_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318776.1"
                     /translation="MFKEGCPGSAEIRSPKPEDIRCQNCGEKIEIWSDEPDTQCRKCG
                     HLNARPIGTSCIEWCAFAKECVGTEKFDKLRKAAKG"
     gene            1543..2556
                     /locus_tag="VFK23_06540"
     CDS             1543..2556
                     /locus_tag="VFK23_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7318777.1"
                     /translation="MNEGGEMMPDTIIARLKDVERTLHEQLRFLQLMIDTIPMPVFYK
                     DLHGVYLGCNKAFEEFLGVPKDRLIGKTVYDVAPKELADNYFQKDKELFDSPGIQVYD
                     FRVQHADGTLRDVTFNKATYTNAQGKTAGLIGVMQDMTERKKAEQVIKRNYDTQTAIN
                     WVLNISLRDISLESVLKQTLDLLLSIPWLSHESRGSIFLVENDPGILVMKAQRGLSGQ
                     VLQQCRTVPFGACLCGRAAARREVQFTDAVDGRHEVRYDGMCPHGHYCVPIMHAKTVL
                     GVINLYLKAGHRQDDREIEFLNAVASALAGIIQRKSTEAERERLIDDLRTVLDAINRS
                     YKE"
     gene            2697..>3795
                     /gene="aspS"
                     /locus_tag="VFK23_06545"
     CDS             2697..>3795
                     /gene="aspS"
                     /locus_tag="VFK23_06545"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET7318778.1"
                     /translation="MTALKKTHSCGDLTRKDAGSVVTLMGWVHTRRDHGGLIFVDLRD
                     RGGITQVVFNPEASSQTHELAHDIRSEYVLAVTGTVAPRPGNTVNPKLPTGEIEVLAN
                     GLEILNTAKTPPFMIEDETDVAEMLRLKYRYLDLRRPSLQKTLITRHRITQTVRRFLD
                     GHGFIDIETPFLTKSTPEGARDYLVPSRVNPGMFFALPQSPQLFKQILMVAGFERYYQ
                     IVKCFRDEDLRADRQPEFTQIDLEMSFIGEDDVISLMEKLLAEVVKEVKGIDIRAPFP
                     RLSYREAMDRYGSDKPDTRFGLELTDISDIAKASDFKVFLQAVELGGQVRGFAAPGMA
                     GLSRKELDDLTNEAKVFGAKGLAWIKSTDKGF"
BASE COUNT          879 a         1075 c         1094 g          747 t
ORIGIN      
        1 gttgcggccg gaggccgcgc catggtatgc taatgccgag gagggagagc atgaaacgat
       61 atatcgccgc ggttctcgca gcaatcatct catccgggtg ctcaggcacg gtaacgaaaa
      121 gcttcaaagt ctttgctgat ccgccggatg ccgacatcag ggtggtttcg ggcgaggacc
      181 tcaaggagct gaaataccgc tcgccggcgg tcgtcactgc cgaggtgccg aaggaccccg
      241 cgctcgcgtc caaggctgtt gtggaggtga gcaaggacag ctataaaccc ttcactctcg
      301 cgcttcgaaa catcagggac ggccagacgc tcaatgtcag gctcgagaag atcgtccaga
      361 gcatgtatcg gtaccggctg gcctatcgcc tggtcgctcc cgcaatttcc gaaacgcttc
      421 agttcaggga cgcgaccatc gccgtatcgt tcacggtcga cggccggtcg atccagatgc
      481 gccttacaaa ccttctcgac aagaatttga agcttctgtg ggagcaggcg cagtatacgg
      541 acgtgaacaa gcagacgtcc cggctgatgc attccggcgt tcgcttccag gaccgcaaca
      601 accctatccc ggagcagatc gtgccgccgc gggcatcgat ccaggagggg gtcattccga
      661 tcaacagggt cgttttttca caggagaaaa aggcttatga gaccaaggcc ctcctcccga
      721 tcgaaagcga gtcaggagct cagttgaagg gcaagacgat caacctgttc atccctattg
      781 aggtggatcg cgcgattatt ccgtacaatt tcaagatcga gataaccgac tcggtgaggg
      841 aagatgtgaa ggggtgaatc cggttataaa caaaaaccga tactcgatgg tcgatgctgg
      901 atgatccagc acccagaaac cagtatccag catccgctat atgacctgca tcatagataa
      961 tgtttctttg ttgtggtaga gtttaatcat gtttaaagaa ggttgtccag gaagcgctga
     1021 aatacgttcg cccaagcccg aggatatcag gtgccagaac tgcggcgaaa agatcgagat
     1081 ctggagcgac gagccagaca cgcagtgcag gaagtgcggg catctgaacg cacggcccat
     1141 cggaacgtcc tgtatcgaat ggtgtgcctt tgccaaggag tgcgtcggca ccgagaagtt
     1201 cgataagctc cgaaaggccg ccaagggtta ggcaggatca cgattccccc gatccacaat
     1261 gaagggttat cgttcgatat atgccccgcg caagcccctt tttttcgcgg tgctccctct
     1321 ccatccgcaa atacagaccc tctgccctcc cgataagaca aatacctcct cttttttccc
     1381 cgtgttattt gccaatcatt tattgaaatc aatactacaa tcaatactac atagactagt
     1441 gcaattttct gttttcagtg ttattatatc gggcatttct tcgcgagtca tctccccaga
     1501 gcggggattc ccggctcatt accaccgggc ggagcaagag acatgaacga ggggggcgaa
     1561 atgatgcctg atacgatcat agcaaggctt aaggatgtag aacggacgct gcacgaacag
     1621 ttgcgctttc ttcaactgat gatcgacacg atccccatgc ccgtgttcta caaggacttg
     1681 cacggtgtct atctgggatg taacaaggcg ttcgaggaat ttctcggcgt accgaaggac
     1741 cggctcatcg gcaagaccgt gtacgatgtt gcccccaaag agcttgcaga taactatttc
     1801 cagaaggaca aggaactgtt cgacagcccc ggcatccagg tctatgattt tcgcgtacaa
     1861 cacgcggatg ggactctccg cgatgtgacc ttcaacaagg ccacctatac caatgcgcag
     1921 ggcaagacgg ccgggctgat cggcgtcatg caggacatga cggaacgcaa gaaggccgaa
     1981 caggtaataa aacggaacta cgatacccag accgcgatca actgggtgct gaacatctcc
     2041 ctgagggaca tttccctgga aagcgtcctc aagcagacgc tcgatctcct tctctccatc
     2101 ccctggcttt cccatgagtc gcggggaagc attttcctgg tcgagaatga tccgggtatc
     2161 ctggtcatga aggcccagcg ggggctgtcc ggccaagtcc tgcagcaatg caggaccgtg
     2221 cccttcggcg cctgtctctg cggccgggcc gcagccagga gagaggtcca gttcaccgat
     2281 gccgtggacg gccggcacga ggtgcggtac gacggcatgt gtccccatgg tcattactgc
     2341 gtccccatca tgcacgccaa gacggttctc ggggtgatca acctctatct gaaggccggt
     2401 caccggcagg acgacaggga aatagagttc ctgaacgccg tggcgagcgc gctggctggg
     2461 atcatccagc gcaagtcgac cgaagcagaa cgcgaaagac tcattgacga cctcaggacg
     2521 gtcctggacg caatcaaccg gtcatacaag gaatagcagt gcccgcttaa tggaaaatcg
     2581 ccggcatcgg tgcggatttg atcatcctga acggaagctt gactttgtgc cgcgcgagat
     2641 gctattatcc tgcctgtttt tactgttacg agaaacatca aaaggggcat tgaccggtga
     2701 cagcgcttaa aaaaacgcat tcctgcgggg acctgacgag aaaggacgcg ggctccgtgg
     2761 tgacgctcat gggatgggtg cacacgcggc gggaccacgg cggactcatc ttcgtggact
     2821 taagggaccg cggcggcatt acgcaggtgg tcttcaatcc cgaggcatcc tcgcagacgc
     2881 atgaactggc gcatgacatc cggagcgagt atgtcctcgc tgtcacgggg acggtagcgc
     2941 ccaggcccgg caatacggtg aaccccaagc tcccgacggg cgagatcgag gtgctggcga
     3001 acggtctcga gatcctgaat acggcgaaga ccccgccctt catgatcgag gacgagaccg
     3061 acgtggccga gatgctcagg ctcaaatacc gctacctcga cctcaggcgg ccctccctcc
     3121 agaagacgct catcacgcgc caccggatca cgcagaccgt acgccgcttc ctggatggcc
     3181 acgggttcat cgatatcgag actcccttcc tcacgaagag cacgcccgag ggcgcacggg
     3241 actatctcgt gccgagccgc gtgaaccccg gaatgttctt cgcgctcccg cagtcgcccc
     3301 agctcttcaa gcagatcctg atggtggcag gcttcgagcg ctactaccag atcgtgaagt
     3361 gcttccggga cgaggacctg cgggccgacc gccagccgga gttcacgcag atcgacctcg
     3421 agatgtcctt catcggcgag gacgacgtca tctcgctcat ggagaagctc ctggcggagg
     3481 tcgtgaagga agtaaagggg atcgacatca gggcgccgtt cccgaggctt tcctatcgag
     3541 aggccatgga ccgctacggg tctgacaagc ctgacacgcg cttcgggctg gagcttacgg
     3601 acatctccga catcgcgaag gcatcggact ttaaagtatt tttacaggcc gtggaactgg
     3661 gcggacaggt caggggtttc gccgctccgg gcatggctgg actttcccgc aaggaattgg
     3721 acgacctcac gaacgaggca aaggtcttcg gcgcgaaggg cctggcctgg atcaagtcga
     3781 ccgacaaggg cttcg
//
LOCUS       DASSLD010000364         4871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1500456, whole genome shotgun sequence.
ACCESSION   DASSLD010000364 DASSLD010000000
VERSION     DASSLD010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500456"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFK23_06550"
     CDS             <1..358
                     /locus_tag="VFK23_06550"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7318779.1"
                     /translation="RAAVLPYIHDMAGAYAAADLVVSRSGATTVAELAVCGKRAVLIP
                     FPYAADNHQEFNARTLAERGQAEIIIQKDLAAERLAGLIKKYASQKAGSTPALRMENT
                     AAEDIARICRSYVQKN"
     gene            342..1727
                     /gene="murC"
                     /locus_tag="VFK23_06555"
     CDS             342..1727
                     /gene="murC"
                     /locus_tag="VFK23_06555"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET7318780.1"
                     /translation="MYKKIKHIHFVGIGGIGMSGIAEVLLNLGYRVSGSDMKESDTTE
                     RLRRLGGEIAIGHRAENVTTPHVVVISSAVKNDNVEVVAAREKQVPVIPRAEMLAELM
                     RLKYGVAIAGAHGKTTTTSMVATVLAAGGIDPTVVIGGKLNSLGTNAKLGQGEFLVAE
                     ADESDGSFLKLSPTVAVVTTIDAEHLDYYRDIDEIKEAFLTFINKVPFYGVSILCLDQ
                     PHIQALIPKVEKRYQTYGMSTQADYQARDLSLRALGSTFRVMYHAEDLGHFELSVPGV
                     HNICNGLAAIAVARELDIDLEVIRKALRAFSGVQRRFQIKGEAGGIIVVDDYGHHPTE
                     VRATLAAAARGLERRVVVVFQPHRYTRTQHLLKEFFTAFNQADKLIIMDIYAAGEKPI
                     PGISGQALYEGIKKFGHKDVTFIPEREKIVEHLLGVLRRGDLMITLGAGDVWKIGEEV
                     VKRLEHGHSRL"
     gene            1708..2616
                     /gene="murB"
                     /locus_tag="VFK23_06560"
     CDS             1708..2616
                     /gene="murB"
                     /locus_tag="VFK23_06560"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET7318781.1"
                     /translation="MDIQDFKGEIVQNEPLSRHTSFGIGGPADFLAYPADRDDLVVLF
                     REIRKRGLASAVIGSGTNLLVRDKGFRGIVVCLKRMTATRVVREYRSIGGAFAVVSVE
                     AGALLAKLLSFSVDEGLTGLEFAAGIPGTVGGAVCMNAGTAAGEMGDVIESVTLLNPD
                     GLLVTRGRDEMGFGYRTSSVPEGHLVLEATVVLRRGEKDRIAAGVKDLLEGRKERQPA
                     GLPNAGSVFKNPQEESAGRLIEQAGLKGKAVGGAQVSEKHANFIVNRGGATAADVLAL
                     MEIVRQTVLDVHGVRLEPEIKIIGED"
     gene            2707..3630
                     /locus_tag="VFK23_06565"
     CDS             2707..3630
                     /locus_tag="VFK23_06565"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET7318782.1"
                     /translation="MALVTNKKIGVLMGGLSSEREVSLASGNAILSALREKGYHPVAI
                     DVGRDVAEKLHAQAVDVAFIALHGKFGEDGAIQGMLEVMGIPYTGSGILASAIGMNKT
                     VSKQVFRSRGLLVGPYEVIYDGDTARAGEVIDGLQFPVVVKPHAEGSSVGVSLVFRKE
                     EVAQALALAFEYGNEILIEKFIKGKEVQVGILGDRALGAIEIVPKRAFYDYKAKYEKG
                     MSDHFFPARIPEAVYERTLDTGLAAHRAIGCRGYSRVDFIIDEAGAPYILEVNTLPGM
                     TATSLLPDIAKGVGISFPDLVEEILRLAVTD"
     gene            3700..4611
                     /locus_tag="VFK23_06570"
     CDS             3700..4611
                     /locus_tag="VFK23_06570"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET7318783.1"
                     /translation="MRDYKNVKVPKSYLAGEKRSSVKRVLVNRTSERTERKRGGTTGV
                     LLKVMAAVLIAGAAVLAWQGYLATMRADMFMVSGVDVRGAKHLGEKDLREIAGLFRGQ
                     NIFRADLGAAVRRARVNPWVKDARIHRSLPNRITMAVTERVPSVILDTGADRFLMDDE
                     GMIVERLAKDNAPAWPLPVVAIRGCRARPGEPVASEGLTEAMQLLAEISSRGGWRPAE
                     VTVRADSPEALSVEYADHEFKIGSGRYGEKLRRLAEVMADVKERGIEIAYVDLRPERQ
                     TAVMPKKVQSSEFGVRSSEKKANKGQR"
     gene            4710..>4871
                     /locus_tag="VFK23_06575"
     CDS             4710..>4871
                     /locus_tag="VFK23_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018869879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET7318784.1"
                     /translation="MVAKKKPDNVLVGLDLGTTKVCAIVGEVKEGGQVDVIGIGISPS
                     HGLKKGVVVN"
BASE COUNT         1018 a         1380 c         1640 g          833 t
ORIGIN      
        1 cagggcagcg gttcttccct acatccatga catggcgggg gcctacgcgg cagcggacct
       61 ggtggtctca cgctccgggg cgaccacggt cgcggagctg gcggtctgcg gaaagagggc
      121 ggtcctcatt ccctttccct atgctgcgga caaccaccag gagttcaatg ccaggaccct
      181 cgctgagcgc gggcaggcgg agatcatcat ccagaaggac cttgccgcgg agaggcttgc
      241 cgggctcata aaaaaatacg ccagtcagaa ggccggttcg acgcccgctc tgcggatgga
      301 gaacaccgcg gcggaagata tcgcgaggat ttgtcggagc tatgtacaaa aaaattaaac
      361 atattcattt cgtcggcatc ggcggcatcg gaatgagcgg cattgccgaa gtgctcctga
      421 atctcggata tcgggtctcc ggatcggaca tgaaggagtc ggacaccacg gagcggcttc
      481 ggaggctggg aggcgagatt gcgatcggcc accgcgcgga gaacgttacg acgccgcacg
      541 tggtcgtgat ctcgtcggcc gtgaagaacg acaacgtgga agtggtggcc gcgcgggaaa
      601 agcaggtccc cgtcattccc cgggccgaga tgctggcgga gctcatgcgc ttgaagtacg
      661 gcgtggccat cgcgggcgca cacggcaaga ccacgacgac ctcgatggtc gccacggtac
      721 tcgcggctgg cggcatcgat cccacggtcg tgatcggcgg gaaactgaac tccctcggca
      781 cgaacgccaa actcgggcag ggagagttcc tcgttgccga ggcggacgag agcgacggga
      841 gcttcctcaa gctttcgccc acggtcgccg tagtcacgac gatcgacgcg gagcacctcg
      901 actactaccg ggacatcgac gagatcaagg aggccttcct cacgttcatc aacaaggtgc
      961 ccttctacgg cgtgtcgatc ctgtgcctgg accagccgca catccaggcg ctcatcccga
     1021 aggtcgagaa gcgctaccag acttacggta tgagcaccca ggcggattac caggcccgcg
     1081 acctctcact ccgggccctc gggtccacgt tcagagtcat gtaccacgcc gaggacttgg
     1141 gccactttga gctttcggtg cccggcgttc acaacatctg caacggcctg gctgcgatcg
     1201 ccgtggctcg ggagctggac atcgatctcg aggtcatccg gaaggcgctc cgggcatttt
     1261 ctggcgtgca gagaaggttt cagatcaaag gagaggccgg cggaatcatc gttgtcgacg
     1321 attacggcca tcaccccacg gaggtcaggg ccacgctcgc ggccgccgcg agagggctcg
     1381 agcggcgggt ggtcgtggtg ttccagccgc accggtatac gaggacccag cacctgctca
     1441 aagaattctt taccgccttc aaccaggcgg acaagctcat catcatggac atctatgccg
     1501 cgggggaaaa gcccatcccc ggcatatccg gccaggcgct ctacgaaggg atcaaaaagt
     1561 tcgggcacaa ggacgtcacc ttcatccccg agcgggagaa gatcgtggag caccttctgg
     1621 gcgtgctcag gaggggcgac ctcatgatca ccctgggcgc cggcgacgta tggaaaatag
     1681 gagaagaggt cgtcaaacgt ctggaacatg gacattcaag actttaaggg cgagattgtg
     1741 caaaacgagc cgctttcgcg gcatacatcc ttcgggatcg gcgggcccgc cgattttctg
     1801 gcctatcccg cagaccgcga cgacctcgtc gtgctgttcc gggagatcag gaaacggggc
     1861 ctcgcctctg ccgtcatcgg cagcggcacg aacctgctgg tcagggacaa agggttccgc
     1921 ggcattgtcg tctgcctcaa gcgcatgacg gcaacccggg tcgtgcggga gtaccggtcg
     1981 atcggcgggg ccttcgccgt ggtctcggtc gaggccggcg ccctgctggc gaagctgctc
     2041 tccttctccg ttgacgaagg cctgaccggg ctggagtttg ccgcgggcat tccgggcacc
     2101 gtgggaggcg cggtctgcat gaacgccggg acggcggcgg gggagatggg cgacgtcatt
     2161 gagtcggtga ccctgctgaa tccggacgga ctattggtca cgcggggcag ggacgagatg
     2221 ggcttcgggt accggacctc ctcggtgccg gagggccatc tggtgctcga agccacggtc
     2281 gttctgcggc gcggggaaaa ggacaggatc gcggccgggg tgaaggacct cctggagggg
     2341 agaaaagagc gtcagcccgc cgggcttccg aatgcgggat cggttttcaa aaatccgcag
     2401 gaagaatcgg cgggaaggct gatcgaacag gcgggactca agggaaaggc cgtgggaggg
     2461 gcccaggttt ccgagaaaca tgccaatttc atcgtgaacc ggggcggggc aacggcggcg
     2521 gacgtgctcg cgctcatgga gatcgtacgc cagaccgtcc tcgacgtgca cggcgtaagg
     2581 ctcgagccgg agataaagat cataggagaa gactaatcaa ttgcggaatt cggaatgcgg
     2641 attgcggaat gcggaataaa aattcgcaag gcgcaaaacc gtactctgcg ataaaaagga
     2701 atttctatgg cactggtgac gaataagaaa atcggggtac tcatgggcgg actctcgtcg
     2761 gagcgggagg tgtcgcttgc ctccgggaat gcgatcctct cggcgctccg ggaaaaagga
     2821 taccatcccg tcgccatcga cgtgggcagg gacgtggccg aaaagcttca cgcgcaggcg
     2881 gtcgacgtgg ccttcatcgc actccacgga aaattcggcg aggacggcgc gatccagggc
     2941 atgctcgagg tgatgggcat cccctacacg ggctcgggca ttctcgcgag cgccatcggc
     3001 atgaacaaga ccgtttcgaa gcaggtgttc cggtcccgcg ggcttctggt cggaccctac
     3061 gaagttattt acgacggcga taccgcgcgg gcgggagaag tcatcgacgg gcttcagttt
     3121 cccgttgtgg tgaaacccca tgcggaaggc tccagcgtgg gggtgagcct cgtcttcagg
     3181 aaagaggaag tggcgcaggc cctggcgctt gccttcgaat acggcaatga gatcctgatc
     3241 gaaaaattca tcaagggaaa ggaagtccag gtgggcatcc tgggggaccg ggccctgggc
     3301 gccatcgaga tcgtgccgaa gcgcgcgttt tacgattaca aggcgaagta cgaaaagggg
     3361 atgtcggacc acttcttccc ggcccggatt cccgaggcgg tctacgaacg gacactcgat
     3421 acaggcctgg ccgcacaccg cgccatcggc tgtcgcggct acagcagggt cgacttcatc
     3481 atcgacgagg ccggggcgcc gtacatcctg gaggtgaaca cgctcccggg catgaccgca
     3541 accagcctcc tccccgacat cgcgaaaggc gtcgggattt cctttccgga tctcgtggag
     3601 gagatactga ggctggcggt cacggactga atgcggaatg cggaataaaa tgccgatcgc
     3661 actgactggt ttttttaaat tccgcattcc gaatttgata tgcgcgacta taagaacgtc
     3721 aaagtaccga aatcctatct cgccggagaa aagcggtcca gcgtgaagcg ggtcctggta
     3781 aacaggacat cggagcggac cgaacggaag cgggggggga cgacaggtgt cctgctcaaa
     3841 gtcatggcag ccgtcctgat cgcgggagcg gctgttctcg cgtggcaggg ctacctggca
     3901 acgatgcggg ccgatatgtt catggtgtcg ggcgtcgatg tcaggggggc caaacacctg
     3961 ggtgagaaag atctcaggga aatagccggg cttttccggg gacagaacat ctttcgggcg
     4021 gacctcgggg ccgccgtgcg gcgggcccgc gtgaacccct gggtgaagga cgcccggatc
     4081 caccggagcc tgccgaaccg catcaccatg gccgttacgg aacgcgttcc ctccgtcatc
     4141 ctggacaccg gcgcggaccg cttcctgatg gatgacgagg ggatgatcgt cgaacggctc
     4201 gcgaaggata acgcgccggc ctggccgctt cccgtcgtgg cgatcagggg gtgccgtgcc
     4261 cggccgggcg agccggtcgc ctccgagggc ttgactgagg cgatgcagct gcttgccgag
     4321 atatccagcc ggggcggatg gcggcctgcc gaggtgacgg tccgggccga ttctcccgag
     4381 gccttgagcg tggagtacgc ggaccatgaa ttcaagatcg gcagcggccg ctacggtgaa
     4441 aagctccgca ggctcgccga ggtcatggcg gacgtgaaag agcgcggcat cgagatcgcc
     4501 tacgtggacc ttcgtcccga gcggcagacg gcggtgatgc cgaagaaagt tcaaagttca
     4561 gagttcggag ttcggagttc ggagaaaaaa gcgaataaag gacaaagata gggagatcga
     4621 gacaggcctg catccgcgtt tgagcggaca tcagcggtca ttgccttgat ctccgaactc
     4681 actactccga actgccttaa ggaggacgaa tcgtggcgaa aaagaagccg gacaatgtgc
     4741 tcgtcggact ggatctcggg acgaccaagg tgtgcgccat cgtcggagag gtgaaggagg
     4801 gcgggcaggt cgacgtgatc gggatcggca tcagtccttc ccacggcctc aagaagggtg
     4861 tagtcgtcaa c
//
LOCUS       DASSLD010000365         4007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_411999,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000365 DASSLD010000000
VERSION     DASSLD010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411999"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFK23_06580"
     CDS             <1..496
                     /locus_tag="VFK23_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7318785.1"
                     /translation="DIFGELLLVEERRPFNALAVTDVLVGVLSREDFVEVLSAIPRVR
                     LNFIRILSRRLAQVEKGITEFNHTRSYQRLAKVLLQICGELGVETPGGVMLRLSLTHA
                     DLADMIGTTRETVTNQLKRFHRMGLVWFHRRHLVVNRRRMADYVQSEGSWRNGEVLEL
                     AKSA"
     gene            599..1273
                     /locus_tag="VFK23_06585"
     CDS             599..1273
                     /locus_tag="VFK23_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7318786.1"
                     /translation="MPLSVTTAVELFQGISETEAQRVERLCTERKYRKGATIFSKGDP
                     SNALFIVKSGKVRILSLSDKGTETIVHILKEGAIFGELLLSEEQRAFTAVAGTDALVT
                     VLPKGSLVELLTAIPTVSKNFVQLLSKRLAKVELEYGDFGHTWSYNRLKKVLLRLCEE
                     HGKETPKGTLIPLRLTHEDLANLIGTTRETVTTQMIRFRRMGLVKRQDRFIVVNKPRL
                     EEFSRS"
     gene            1534..2031
                     /locus_tag="VFK23_06590"
     CDS             1534..2031
                     /locus_tag="VFK23_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7318787.1"
                     /translation="MYSKILVALDGGELSERAVQQAEVIGQATKAEVILFQVVQNPIR
                     SVPIAAGQKEVTKATQDVTDKADAYLQKMAAPLKAKGIKVRCETEVGEPTPRILGKAH
                     REDVDLIVMSTHYEGELHKLVTGSIAEKVLLGTKRPVLLVKPERVAIAHHVDEQEAIG
                     RSSHK"
     gene            complement(2122..2973)
                     /locus_tag="VFK23_06595"
     CDS             complement(2122..2973)
                     /locus_tag="VFK23_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET7318788.1"
                     /translation="MTPLAPLETLLDRIGGDDLPLPAGLATLYGPLRFPLKKVRPYVI
                     GNFVTTMDGVASLSAPGFAGGGPISGSNPHDRMVMGLLRSVADAVIVGAGTLRSVPNH
                     LWTAEYIYPPLSDSYRQLRIALGKKASPLNVIVTARGEIDPGLPVFRSEAVKVLIVTN
                     PEGARRIRLRDLPASVVIGEVGEGGGAISAREVLSAVGRILPGEILLVEGGPRLMGDF
                     FAGKCLDELFLTLAPQVAGRDGSVERPGFVAGKRFGPERPLWGTLAGVKRAGSHLFLR
                     YSFETEG"
     gene            complement(2987..3655)
                     /gene="folE"
                     /locus_tag="VFK23_06600"
     CDS             complement(2987..3655)
                     /gene="folE"
                     /locus_tag="VFK23_06600"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HET7318789.1"
                     /translation="MSSDHGHDPRECLSDMSTNSRHVSAERMRQFEGYVTEILSAFGM
                     DPDTPATRDTPRRFLQAMFDSTDGYEGDPKLITVFDTECRGGPDCRLSQVVEGPIQFF
                     SLCEHHALPFYGRAYVGYIAHEQIIGLSKLTRLVRLFAKRFTVQERIGQQIADSLVSM
                     LHPHGVAVYLDAHHLCTQMRGVRETAPLTRTTFWRGNYESDPSLRTEFYVACGLHRFE
                     PGSR"
BASE COUNT          867 a         1191 c         1225 g          724 t
ORIGIN      
        1 ggacatcttc ggggaactcc tcctcgtcga ggagaggcga ccgttcaatg cactggccgt
       61 gacggatgtc cttgtcggcg tcctttcacg ggaagatttt gtcgaggtcc tgtcggcgat
      121 tccccgggtc cgcctgaatt tcatccggat tctgtccagg cgcctggccc aagttgaaaa
      181 ggggattacg gaattcaacc acaccaggtc ttatcagcgg ttggcaaagg tcctccttca
      241 gatatgcggt gaactcgggg tggaaactcc cggcggggtc atgctccgcc tgtcgctgac
      301 ccacgcggac cttgcggaca tgatcggcac cacccgggaa accgtcacga accagctgaa
      361 acgctttcac cggatgggtt tggtatggtt ccataggcgt cacctcgtcg tgaaccgccg
      421 gcgtatggcc gactatgttc aatcagaggg gagctggcgg aacggagagg tgctcgagct
      481 ggcgaaaagc gcatagggaa aggggaccct ccaaggagtc gagagtccgg cgccgccttg
      541 cggtggcgtt cggcgactcg tcgttgtttt tcgccggggg cggttgtaat gtggggttat
      601 gccgttatcc gttaccacag ccgtcgagtt gttccaggga atttccgaaa ccgaggcaca
      661 gagggtcgaa cgcctgtgca ccgagcggaa gtatcggaag ggcgccacga ttttctccaa
      721 gggcgacccg tcgaacgctc tcttcatcgt caagagcgga aaggtgagaa ttctctccct
      781 ctcggacaaa gggacggaga cgatcgtgca catcctcaag gagggcgcga tcttcgggga
      841 actcctcctt tccgaggagc agcgggcctt caccgccgtc gccggcacgg acgccctggt
      901 caccgtcctc ccgaagggaa gcctggtgga actccttacc gccatcccca ccgtatcgaa
      961 gaatttcgtc cagctgctct cgaagcggct ggcaaaggtg gagctggaat acggggattt
     1021 cgggcacacc tggtcctaca accgcctcaa gaaggttctg ctccggctct gcgaagagca
     1081 cggcaaggag accccgaagg ggaccctgat ccccttgcgc ctgacgcacg aggacctggc
     1141 gaacctgatc gggaccaccc gggagacggt cacgacccag atgatccggt tccggcgcat
     1201 ggggcttgtg aagcgccagg accgcttcat cgtcgtcaac aagccccgac tcgaggagtt
     1261 cagccgctcc tagtctctgc ctgcagcagc ggtacgagtc ggaacgtgtc ggccagatac
     1321 actccgttta cctgcaacca gagaaaacca agaaaatcaa tttagtacaa gatttcacaa
     1381 accttccacc atgtcatgtc atcttacagt catgtaggaa aggaagagtg tacgagcgga
     1441 atgcgtcgca gtgacgacgc ggttcacgag ggaggagcga ttccgtcgcc catcaatata
     1501 atttacccgg aagaacaaaa aaggaggaag aaaatgtatt ccaagatcct ggtggcgctt
     1561 gacggggggg aactatcgga acgggccgtc cagcaagcgg aagtgatcgg acaagccacg
     1621 aaggcggaag tcatcctttt ccaggtggtt caaaatccga tccgctcggt cccgattgct
     1681 gcgggccaga aggaagtaac aaaagcaaca caggacgtga cagacaaggc tgacgcttat
     1741 cttcagaaga tggctgcccc gttgaaggca aagggaatca aggttcgttg cgagacagaa
     1801 gtgggcgaac caacgccccg tattctcggc aaagcccaca gggaggacgt ggatctcatc
     1861 gtgatgagca cccactacga gggcgaactc cacaagttgg tcaccgggag catcgcggag
     1921 aaggtgctgc tcgggacgaa gcgccccgtg ttgctggtca agccggaaag ggtcgcaatt
     1981 gcacaccacg tggatgagca ggaggcgatc ggaagatcgt cgcataagta attccggctg
     2041 gaacgcttgc ggtcgacacc cggcttctcg tccacgaggg aagccgggaa ctattacggt
     2101 ttctcccgat ccaaccgggg atcaaccctc tgtctcgaag gaatacctta aaaacaggtg
     2161 gctgccggca cgtttcacgc cggcaagcgt tccccacagg gggcgctccg gtccgaaccg
     2221 cttccccgca acgaatccgg gacgctcgac agatccatcc cgcccggcca cctgcggagc
     2281 gagcgtcaaa aaaagctcat ccaggcattt cccggcgaaa aaatccccca tcaacctcgg
     2341 tccgccttcg acaaggagga tttcacccgg aagaatacgg ccgaccgcgg aaagaacctc
     2401 ccgcgcgctg atcgcgcccc ctccctcccc cacttccccg atgacgacgg aggcgggaag
     2461 gtcgcgcaag cgaatccggc gggccccctc cgggttcgta acgatcagca ccttcaccgc
     2521 ttcggaccgg aagacgggaa ggcccgggtc gatctcgccc cgcgcggtga cgatgacatt
     2581 caacggggag gccttcttcc ctaatgcgat ccgcagctgc ctgtacgagt ccgacagcgg
     2641 cgggtagata tactccgcgg tccaaaggtg gttcgggacg gagcgaagcg tcccggcgcc
     2701 cacgatcacg gcatccgcga cggatcgcaa cagtcccatg accatcctgt cgtgcgggtt
     2761 ggaaccactg atggggccgc ctcccgcaaa accgggggcg gagagggatg caacgccgtc
     2821 catcgtggtg acgaagttgc cgatgacgta cggacggact ttcttcagtg gaaatcgaag
     2881 ggggccgtag agcgtcgcca gcccggctgg cagaggaaga tcgtccccgc caatccggtc
     2941 aaggagggtt tccaggggcg caaggggggt catcgcacgc cgccggttac cgggagccgg
     3001 gctcgaagcg atgcaggccg cacgcgacgt aaaattcggt ccgcagcgac gggtcgcttt
     3061 cgtaattccc tcgccagaat gtcgtccgcg tgagcggcgc ggtttcccga actccccgca
     3121 tctgggtgca gaggtggtgt gcgtccaggt acaccgcgac gccgtgcgga tgaagcatgg
     3181 agaccaggga gtcggcaatt tgctggccga tccgctcctg cacggtgaac cgtttcgcaa
     3241 acaggcgcac cagcctcgtg agcttggaga ggccgatgat ctgctcgtgg gcgatgtacc
     3301 cgacgtaagc gcggccgtaa aaaggaagcg catgatgttc gcagaggctg aagaactgga
     3361 tcggtccttc cacgacctgg ctgaggcggc agtccggtcc tccacggcac tcggtatcga
     3421 ataccgtgat cagtttcgga tccccctcgt acccgtcggt gctgtcgaac atggcctgga
     3481 gaaagcgtct cggggtgtca cgcgtggcag gcgtgtccgg atccatcccg aaggccgaga
     3541 ggatttccgt cacgtagcct tcgaactgcc gcatccgttc cgccgaaaca tggcggctat
     3601 tcgtgctcat gtccgataaa cattcccggg ggtcatgccc atggtccgaa ctcatcggcc
     3661 ctcctcggag cgaaaggtaa tcccatatta cccccctcac gaataaataa cctcattgtt
     3721 cgacctttca caaacgttcc tccccctcat tgttatctta tggtcagaaa gggggctctt
     3781 cagggaaccc ccccgatcga aggaaacgac ggagtaataa aggaggtggc cgccatgaag
     3841 tggatccggg agattctccc aaggggaaaa gaggcggcgt tcgcatccgg actgctgctc
     3901 ctctacgcga tggaactctt cctctctccg gacccggcgt ggacgatgtg ggcggcacag
     3961 atggcaatgg gcgtcctgta cgtcccggat gcaccgacgt tgcagtc
//
LOCUS       DASSLD010000366         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_400682,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000366 DASSLD010000000
VERSION     DASSLD010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400682"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..481
                     /locus_tag="VFK23_06605"
     CDS             104..481
                     /locus_tag="VFK23_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET7318790.1"
                     /translation="MAKLTIADLKKIKEDYHQGLREGGFRVRITVHMGTCGIAAGARP
                     IMNVVMEELAKSGLKDVAITTSGCAGLCSKEPMATIELTGEPPVKYISLTEDKMRKIF
                     AEHVAGGKPVTEYALVAGHETTY"
     gene            570..>2261
                     /gene="nuoF"
                     /locus_tag="VFK23_06610"
     CDS             570..>2261
                     /gene="nuoF"
                     /locus_tag="VFK23_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HET7318791.1"
                     /translation="MICAGTGCVANGSLKVKDALETELIKRNLQNEIKIVLTGCNGFC
                     AKGPVMVVYPEDIFYQQVKPEDVPHLVEEHFIKGRPVQKFLYTPPTDKKTIPVMNEIP
                     FFKHQVLRALRNRSLIDAEKIEEYIARDGYMAAAKALTEMTPEQIVAEVKKSGLRGRG
                     GAGFLTGLKWELCARVQGDIKYILCNGDEGDPGAFMDRSVMEADPHSVLEGMIIAAKG
                     INAHYGYIYVRAEYPLAVHRLQLAIDQAKERGLLGKDILGSGFDLDIEIYQGAGAFVC
                     GEETALMASIEGKRGMPRAKPPFPAVKGLWQRPSVLNNVETFANVPPIILNGADWFAS
                     LGSEKSKGTKVFALSGALNNIGLVEVPMGIPLKTIIFDIGGGIKGNRKFKAVQMGGPS
                     GGCIPHSLIDTPVDFENINKTGAIMGSGGMVVMDELTCMPAMAKFFLEFTEDESCGKC
                     VPCRIGNKTLLNILTRITNGEGREGDIELLLALSQQIKDTSLCGLGQTSPNPVLTTIK
                     YFRDEYEAHIKDHKCPARVCTPLIEFKVNEEKCKKCGMCFKNCPVGAITWEKGKVA"
BASE COUNT          521 a          653 c          704 g          383 t
ORIGIN      
        1 caggacacct acggcgacgt ggcggcgacc aaggtgatgg acattatcaa aaagtacgaa
       61 tagcctctga attcggaatg cggaatgtaa cggcgggggt attatggcta aactgacgat
      121 tgcggattta aaaaagatca aggaagacta ccaccaggga ctgcgcgagg gcgggttccg
      181 ggtcagaatc acggtgcata tggggacctg cggaatagcc gcgggcgccc ggcccatcat
      241 gaatgtcgtc atggaggaac tggcgaagtc cgggctcaag gacgttgcca ttaccacgtc
      301 gggctgcgcg ggcctttgca gcaaggagcc gatggccacg atcgaactca ccggcgaacc
      361 gccggtgaaa tatatctcgc tgaccgaaga caaaatgcgc aaaatcttcg ccgaacacgt
      421 ggcggggggc aagccggtga cggagtacgc ccttgttgca gggcatgaga cgacctatta
      481 aataaattcg gtttaaaccg tttgaaccgc tcaaaccgct taaactgttc aatggagtaa
      541 ctgtggaaac gacctatcgc tcaaacataa tgatctgcgc aggaacgggc tgcgtggcaa
      601 acggatcgct caaggtgaaa gacgcgctcg agacggagct catcaagcgc aacctccaga
      661 acgagatcaa gatcgttctg accgggtgca acgggttctg cgccaagggc cccgtcatgg
      721 tggtctaccc cgaggacatc ttctaccagc aggtgaagcc cgaggacgtc cctcatctcg
      781 tggaggagca cttcatcaag ggcagaccgg tccagaaatt cctgtatacg ccgccgacgg
      841 acaagaagac gatcccggtg atgaacgaga tccccttctt caagcaccag gtgctccgcg
      901 cgctccggaa caggagcctc atcgacgccg aaaagatcga ggagtacatc gcccgcgacg
      961 gctacatggc ggcagccaag gccctgaccg agatgacccc ggagcagatc gtcgccgagg
     1021 tgaagaaatc gggcctccgc ggcaggggcg gcgcgggctt tctgacggga ctcaagtggg
     1081 agctctgcgc gcgcgttcaa ggcgacatca agtacatcct gtgcaacggc gacgagggcg
     1141 acccgggcgc gttcatggac cggagcgtca tggaagcaga cccgcattcg gtgctggaag
     1201 gcatgatcat cgcggccaag ggcatcaacg cccattatgg ctacatctac gtccgggccg
     1261 agtatccgct ggccgtgcac cgtctccaac tggccataga ccaggccaag gagcggggcc
     1321 ttttaggcaa ggacattctc ggttccgggt tcgatctcga catcgagatc taccaggggg
     1381 cgggcgcctt cgtgtgcggc gaagagacgg cgctcatggc ctccatcgag ggaaagcgcg
     1441 gcatgccgag ggcgaaaccg ccgttccccg cggtcaaggg tctctggcag cgtccgtcgg
     1501 tcttgaacaa cgtcgaaacc tttgcaaacg ttccgccgat catcctgaac ggcgcggact
     1561 ggttcgcgag tctcggctcc gagaagagca aagggacgaa agtctttgcg ctctccggcg
     1621 cgctcaacaa tatcggcctc gtcgaggtgc ccatgggcat cccgctcaag accattatct
     1681 tcgacatcgg cggcggtatc aagggaaacc gcaagttcaa ggccgtgcag atgggcggcc
     1741 cgtcgggcgg ctgcatcccc cattcgctca tcgacacgcc cgtggatttc gagaacatca
     1801 acaagaccgg agcgatcatg gggtccggcg gcatggtggt gatggacgag ctcacctgca
     1861 tgccggccat ggcgaagttc ttcctcgagt tcaccgagga cgagtcgtgc ggcaagtgcg
     1921 tgccctgcag gatcgggaac aagacgctct tgaacatcct gacccggatc acgaacggcg
     1981 agggccgcga aggcgacatc gagctcctgc tcgccctttc ccagcagatc aaggacacgt
     2041 cgctctgcgg cttgggccag acctcgccca accccgtcct gaccacgatc aagtatttcc
     2101 gggacgagta cgaggcgcac atcaaggacc acaaatgtcc ggcgcgggtt tgtacgcccc
     2161 tcatcgagtt caaggtaaac gaggagaagt gcaagaagtg cggtatgtgc ttcaagaact
     2221 gcccggtggg cgccatcacc tgggagaagg gcaaggtcgc g
//
LOCUS       DASSLD010000367         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_226672,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000367 DASSLD010000000
VERSION     DASSLD010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226672"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="VFK23_06615"
     CDS             <1..1380
                     /locus_tag="VFK23_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HET7318792.1"
                     /translation="NKKRELVIMKKLLKRLYDFFARDLWAVGRPSPRGLHALLIGALR
                     IVTVVVKDVIYGEITLRATGLVYSTLLSLVPLLAVGFSVLKAFGVHNALEPFLLNLLT
                     PLGSTAADLAQRIVGFVDNMKVGVLGFVGLGLLIYTVVALIQKIEEAFNVIWRVRSPR
                     SILQRFSDYLSVVLVGPVLVASALGLTASLMSTTLMQKLVAVGPISDLVFTAGKLLPY
                     VLVCAAFAFIYIFVPNTKVRFVPAITGAVVGGVLWETVGWGFASFIVASTQYGAVYSG
                     FAALILFMLWLYLNWFILLVGAEVAFYCQNPQYLRLSGNGWIPDARLIEQTALAVMFL
                     ISRSFHTGGQSWTVDMLAQRLGLSPERVGEIVDALKKSRLIAETAEVPPALLPARDPG
                     TIPLRNVYEAVRGSGCGKAGESIARIDAVESIAVLVEEAIGGSLDGTTIGDLVRSPGN
                     AYEDTKTGR"
     gene            1518..1910
                     /locus_tag="VFK23_06620"
     CDS             1518..1910
                     /locus_tag="VFK23_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318793.1"
                     /translation="MMILVYVLFALTVSTVFTALFSARIPENRRGDAFIGFFAVLMVL
                     AWAADTWLLPALAAGPGRSWVPIVMLIIFAGVLASTAVLAVRTPRPFQHAVARHDGRL
                     NGEAAVFDLVLWAALLVLGMALLKSLTP"
     gene            1975..2250
                     /locus_tag="VFK23_06625"
     CDS             1975..2250
                     /locus_tag="VFK23_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HET7318794.1"
                     /translation="MKSLLTSSLRPARRVIVAVIGLTVLLMGVAMIILPGPAVVVVPV
                     GLAILGTEFVWARRLLGKARAWFTNGTPEQMKRGGQKTGQGGTEGEG"
     gene            complement(2285..>2625)
                     /locus_tag="VFK23_06630"
     CDS             complement(2285..>2625)
                     /locus_tag="VFK23_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET7318795.1"
                     /translation="PPGRHCFEFRDPSWFTQEVYDALADAKASFCIYQLAGLQSPGEI
                     TADFVYVRLHGPAREAYRGRYPAQVLRGWAGAFAGWTHAGKDVYCYFDNDEAGYAALD
                     AAKLREMTGP"
BASE COUNT          467 a          762 c          833 g          563 t
ORIGIN      
        1 aataaaaaaa gagagctggt catcatgaaa aaactgctga aacggctgta cgactttttt
       61 gcgcgcgacc tgtgggcagt aggccggccg tcgcccagag ggcttcatgc cctgctgatc
      121 ggagctctcc ggatcgttac cgtcgttgtg aaggacgtca tttacggcga gataacactg
      181 cgggccacag gcctcgtgta ctcgacactc ctgtcgctcg tgcccctgct cgcggtcggt
      241 ttctcggtgc tcaaggcctt cggcgtgcat aatgcgcttg agccgttcct gctcaatctc
      301 ctgaccccgc ttgggtccac ggccgcggac ctggcgcagc gaatcgtggg tttcgtcgac
      361 aacatgaagg tgggcgtgct gggattcgtc ggcctgggac tgctcatcta caccgtcgtc
      421 gccctcatcc agaagatcga ggaggcattc aacgtgatct ggcgggttcg gagcccgcgg
      481 agcattctgc agcgtttcag cgactacctg agcgtcgtcc tcgtcggccc ggtgcttgtc
      541 gcgtccgccc tgggcctgac ggcttccctc atgagcacga ccctcatgca aaaactcgtt
      601 gccgtcggac cgatcagcga tctcgttttc acagcaggaa agctcttgcc ttacgtcctg
      661 gtttgcgcag ctttcgcctt tatatacata ttcgttccga acaccaaggt ccgcttcgtc
      721 cccgccatca cgggcgctgt tgtaggcggc gtactctggg agacggtggg atggggcttc
      781 gcctccttca tcgttgcctc tacacagtac ggcgcggtct attccggttt cgccgcactg
      841 atcctcttca tgctttggtt gtatcttaac tggttcatcc tgctcgttgg cgctgaagta
      901 gcgttctatt gccagaaccc gcagtatctc aggctctccg gaaacgggtg gatacccgac
      961 gccaggctga tcgagcagac ggcgctcgcc gtcatgtttc tcatcagcag gagctttcac
     1021 acaggcgggc agtcgtggac tgttgatatg ctggcacagc gcctcggtct ttcccctgag
     1081 cgggtcgggg agatcgtgga tgcactcaaa aaatcgcgat tgatcgccga gacggcggaa
     1141 gtcccccccg cactgcttcc ggcaagggac cccggcacca tcccgctccg gaacgtctac
     1201 gaagcggtgc gcgggtccgg ctgcgggaag gccggggagt ctatcgcccg gattgacgca
     1261 gtcgagagca tcgctgtttt ggtcgaggaa gcgatcggcg gcagtctgga cggcacgacg
     1321 atcggcgacc tggtccgttc tccggggaac gcgtatgaag atacaaagac gggaaggtag
     1381 gccgacggga tggtaagagc ggggaggatg cgaccgcaat cggttaaacc tgcatgagtg
     1441 ctaaaatatg agtaggagcg gttgccgcgg tcctgttgat gcggcgggtt cccgccccta
     1501 agggaatgga ggcgatagtg atgatacttg tgtatgttct tttcgcgctg acggtatcga
     1561 cagtttttac cgcgcttttt tctgcacgca tcccggaaaa caggaggggt gatgcattca
     1621 tcgggttctt tgccgtactc atggtgctgg cgtgggcggc cgatacgtgg ctgctcccgg
     1681 cgctggccgc aggaccggga aggtcttggg tcccgatcgt gatgctgatt attttcgcgg
     1741 gagtgctcgc gtcgaccgcc gttctcgcgg tccgcacgcc gcgtcccttc cagcatgcgg
     1801 tggcccgtca tgacgggagg ctcaatggag aggcggcggt cttcgacctc gttctctggg
     1861 ccgcgctgct ggtcctgggc atggctcttt tgaaatctct cactccctaa gcgttctttg
     1921 tcgcgggggt tacaggcatc gcctcatgaa cagggttgac agagacgagt gtgaatgaaa
     1981 tcgctgctga ccagtagtct cagaccggca aggagggtca tcgtcgccgt aatcggttta
     2041 acggtgctcc tcatgggtgt cgccatgatt attctgccgg ggccggccgt agtggtagtc
     2101 cctgtcgggc tggcgattct gggcacggag tttgtctggg cgcggagact tctggggaag
     2161 gcgcgggcgt ggttcacgaa cgggacgccg gagcagatga aaagaggcgg acagaaaacc
     2221 ggtcaaggcg gtacggaggg tgaaggatga attccgcagc ggcataagct ccgtaaatcc
     2281 acgttcatgg gccggtcatt tcccgcagtt tcgccgcgtc cagcgcggcg tacccagcct
     2341 cgtcgttgtc aaaatagcaa tagacgtcct tgcccgcgtg cgtccagccc gcaaaggcgc
     2401 ctgcccatcc gcggaggacc tgagccgggt accggccccg gtaggcttca cgggccggac
     2461 cgtgaaggcg cacatacacg aaatccgccg tgatctcccc gggagactga agccccgcga
     2521 gctgatagat gcagaacgac gccttcgcgt ccgccagcgc atcatagacc tcctgcgtga
     2581 accagctcgg gtcccggaac tcgaagcagt ggcgccccgg gggca
//
LOCUS       DASSLD010000368         2589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1309339, whole genome shotgun sequence.
ACCESSION   DASSLD010000368 DASSLD010000000
VERSION     DASSLD010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309339"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2589
                     /locus_tag="VFK23_06635"
     CDS             <1..>2589
                     /locus_tag="VFK23_06635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HET7318796.1"
                     /translation="EILKDAGAASDCSLAVTGSAGKLISGILGIGSLNEVVAQAYAVR
                     KLHRRIRTVIEMGGEDSKLILLDEGGVKDFSMNSACAAGTGSFLDQQAERLRLNIEEF
                     SELSLRSKKPPRIAGRCSVFAKSDMIHLQQIATPIEEIVAGLCFAVARNFRGSIARGR
                     SIVPPVSFQGGVAANKGMVRAFREVLDLDELFVPPDFALMGALGAAMRAADEGTGAVF
                     RLDPLEEYLQTTWDSAAGHPPLLQEGDAFFARHVGDSRDVVYSLPAAGPRVKVYMGID
                     IGSISTNVAVIDAGGRLLSKRYLMTAGRPIEAVRQGLRETAAELGDRVEIAGVGTTGS
                     GRYMIADYVGADIVKNEITAQATAAAFIDPTVDTIFEIGGQDSKYISLEDGVIVDFEM
                     NKACAAGTGSFLEEQAEKLSVSVKGEFAECALSSACPAKLGERCTVFMENSLMASLQK
                     GTPKNDLFAGLSYSIVQNYINRVVAGKRIGKNIFFQGGVAFNKSVVAAFEKYLGQHIT
                     VPPNHDVTGAIGMALIAMRYMLSEGRSVSSFKGFDLAERRYEISSFGCSGCSNLCEIN
                     RVTMEGEQQPLFYGGRCEKYDINRKDTSRIPDLFKFRDELLWKAVRDHEERRNRRKGN
                     GFRARIGIPYLFSFNDYLPFWVTLLASLGFDPEVSPRPNRQIVDLGLESVLSEACFPV
                     KVAIGHIRYLKEQGVDAVLLPSFVNLNGKDDEYAAGAACPHTQSIPYVGRVAVPGVEI
                     LAPVVDRSRDTEKLANELRRTLRRYGVTKQEIRTALASAGKAQAEFQDALRNKGKEVL
                     AGLTGKAVAIVGRSYNALERGMNLRIPEKLATLGVLAVPMDMLPLGDYRIAGEWPGMY
                     WRNGQRM"
BASE COUNT          534 a          756 c          839 g          460 t
ORIGIN      
        1 gaaattctaa aggatgccgg ggcggcttcc gactgctccc tggccgtaac cggatcagcc
       61 ggaaaactca tttccggaat actgggaatc gggtcgctca acgaggtcgt tgcgcaggcc
      121 tatgccgtac gaaagctcca ccgccgcatc cggaccgtca ttgagatggg cggtgaagac
      181 tcgaagctca tcctgctgga cgagggcggg gtaaaggact tctccatgaa ttcggcgtgc
      241 gcggccggga cggggtcctt tctcgaccag caggcggagc ggctgcgcct caacatcgag
      301 gagttcagcg aactgtcgct cagatcgaag aagccgcccc gcatcgcggg ccgctgcagc
      361 gtgttcgcca agtccgacat gatccacctt cagcagatag ccacgcccat tgaggagatc
      421 gtggccgggc tctgtttcgc cgtggccaga aacttcaggg gcagcattgc ccgcggcagg
      481 agcatcgtcc ctcccgtgtc gtttcagggc ggtgtggcgg cgaacaaagg aatggtgcgg
      541 gcgttccggg aggtccttga tctcgatgaa ctttttgtgc cgcctgattt cgccctcatg
      601 ggcgcccttg gcgcggccat gagggccgcg gacgagggca cgggagcggt gttcaggctc
      661 gatccccttg aggaatacct ccagacgacc tgggattccg cagccggcca tcccccgctc
      721 cttcaggagg gggacgcctt tttcgcacgg cacgtcgggg actcgaggga cgttgtctat
      781 tcgctgcctg cagcaggtcc gcgggtaaaa gtctacatgg gcatcgacat cggctccatc
      841 agcacgaacg tggccgtgat cgacgccggc ggaaggctcc tctccaaacg ctacctcatg
      901 acggcgggca ggcccatcga ggcggtgcgg cagggcttaa gggaaacggc cgcggagctg
      961 ggcgaccgcg tggagattgc cggcgttggc acgacgggct ccggcaggta catgatcgcg
     1021 gactatgtgg gagcggacat cgtgaagaac gagatcacgg cccaggccac ggccgccgca
     1081 ttcatcgacc ccaccgtgga caccatcttc gagatcggag ggcaggactc gaagtatatc
     1141 tcgctcgagg acggcgtgat cgtcgatttc gaaatgaaca aggcctgcgc cgcgggcacc
     1201 gggagctttc tcgaagagca ggcggagaag ctcagcgtgt ccgtgaaagg agagtttgcc
     1261 gagtgcgccc tttcctccgc ctgccccgcg aagctcggcg agcgctgcac cgtattcatg
     1321 gagaactccc tcatggcgag cctccagaaa gggaccccga aaaacgacct gttcgccggc
     1381 ctgtcctatt ccatcgtcca gaactatatc aatcgcgtgg tggccggaaa gaggatcggg
     1441 aagaacatct tcttccaggg aggcgtcgcc ttcaacaagt ccgttgtggc cgctttcgag
     1501 aaatacctcg gccagcatat aacagtcccg ccgaatcatg atgtaaccgg cgccatcggc
     1561 atggccctca tcgccatgcg atatatgctg tccgagggca ggagcgtttc gagcttcaag
     1621 ggattcgacc ttgccgagcg ccgttacgag atatcctcat tcggctgcag cggctgttcc
     1681 aacctgtgcg agatcaaccg cgtgacgatg gagggcgagc aacagccgct tttctacggc
     1741 gggagatgcg aaaagtacga cataaacagg aaggacacga gccgtatccc ggacctcttc
     1801 aagttccgcg acgagcttct ctggaaggcg gtcagggacc atgaggaaag acgtaacagg
     1861 agaaagggga acggcttccg ggcaaggatc gggataccct acctgttctc gttcaacgac
     1921 tatcttccct tctgggtaac gctccttgca agcctcgggt tcgatccgga agtttccccg
     1981 cggcccaacc gtcaaatcgt ggacctgggt ttggaaagcg ttctctccga ggcgtgcttc
     2041 ccggtaaagg tggccatcgg gcacatacgg tacctgaagg agcagggcgt ggatgccgtc
     2101 ctgctgccga gcttcgtgaa tctgaacggg aaggatgatg aatatgcagc gggtgccgcc
     2161 tgccctcata cccagagcat tccgtacgta ggcagggtgg cggtgccggg cgtcgagatc
     2221 ctcgcccccg tcgtggacag gagccgggac acagagaagc ttgcgaacga gctcagacgg
     2281 acgctcaggc ggtacggcgt cacgaagcaa gagataagga cggccctcgc ctcagcagga
     2341 aaggcgcagg cggaatttca ggacgctctc cgcaacaagg gaaaggaggt gctcgcggga
     2401 cttacgggca aggccgtcgc gatcgtgggc agatcgtaca acgccctgga gcgggggatg
     2461 aacctgcgaa tacccgaaaa gctcgcaacg ctcggcgtgc tggccgtccc catggacatg
     2521 cttccgctcg gggattatcg catcgccggg gaatggcccg gaatgtactg gaggaacgga
     2581 cagaggatg
//
LOCUS       DASSLD010000369         3604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_570551,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000369 DASSLD010000000
VERSION     DASSLD010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570551"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(319..504)
                     /locus_tag="VFK23_06640"
     CDS             complement(319..504)
                     /locus_tag="VFK23_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET7318797.1"
                     /translation="MYMVTIDKGKCDGDGTCVNMCPQSVFKVDGGKADPINMSECINC
                     QTCVENCPQQAITVNEI"
     gene            complement(667..1839)
                     /gene="nrfD"
                     /locus_tag="VFK23_06645"
     CDS             complement(667..1839)
                     /gene="nrfD"
                     /locus_tag="VFK23_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET7318798.1"
                     /translation="MLEKAFEGGKSYWGWLCFLGALALVGVYFYLQQFQYGLGITGLH
                     RDVSWGLDIGQFTFLVGVAASAVMLVIPYYLHDFKKFGKIVILGEFLAISAVTMCILF
                     IFVDMGQPMRIMNVVLHPTPNSVMFWDSVVLVGYLCINLLVGWVTLSSDRKGTPPPKW
                     IKPWIYLSIPWAVSIHTVTAFLYAGLPARHFWLSAVMAARFLASAFAGGPALLIILSL
                     IVRKISKFDPGKEAIQAVGKIVAYAMLANVFLLGLEFFTAFYSGIPGHATPFKYLYVG
                     LEGHTELVKLMWTSTILAAASIVILVFPQARKKEGLLASACVMLFISLWIDKGFGLVI
                     GGFVPNPFEEVHPYWPTMPEALITLGVWSIGFLVLSMLYKIAISVREATAGIEIEH"
     gene            complement(1832..2668)
                     /locus_tag="VFK23_06650"
     CDS             complement(1832..2668)
                     /locus_tag="VFK23_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7318799.1"
                     /translation="MSMDRRKFLKIAGISSILGFGASVASATHTIWQKGLEPPQIEPS
                     KEGLVGKRWAMVVDMSKFKTEEDIKKVTDACHHTHNVPDFRNPDGTVDKKFEIKWIWT
                     EPYEHTFPAAENQYMSEHVKEMPFLVLCNHCENPPCVRVCPTKATFKLTNGITMQDMH
                     RCIGCRFCMAACPYGSRSFNWIDPRAAKALKPEFENKEYPCRTKGVVEKCTFCSERLQ
                     AGKIPACVEAAPKGAMFFGDIEDPNSEVRKIVTSQLTVRRKPELGTGPAVYYLIGGSE
                     HA"
     gene            complement(2665..3093)
                     /gene="dsrJ"
                     /locus_tag="VFK23_06655"
     CDS             complement(2665..3093)
                     /gene="dsrJ"
                     /locus_tag="VFK23_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrJ"
                     /protein_id="HET7318800.1"
                     /translation="MKLYDGGKIIIGLAVFLAFALFPFYNNLGKVNAKPEPKTDTPAI
                     QEWEKLYGKKECVESKEYMRSEHMQMLNNWRDAVVRDMYRQYVSQTSGKKFNMSLQNG
                     CMKCHSNKKKFCDECHNYMAVKPYCWDCHIQPQEKEEVKS"
     gene            complement(3093..>3604)
                     /locus_tag="VFK23_06660"
     CDS             complement(3093..>3604)
                     /locus_tag="VFK23_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007781654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET7318801.1"
                     /translation="AEFTADLIRNNKLNLDPSRNDDLIVTWHDSCNTSRGMGLLEEPR
                     YVLKSVVNHFHEMPEDTIREKTFCCGSGTGLNASEDMELRMKGGFPRANAVKFVAEHY
                     GVNMLANICAIDRATLKALMEYWVPSVNVCGLHELVANALIMKGEKPRETNLRGEPLP
                     AKAQKEVKA"
BASE COUNT          734 a         1041 c         1038 g          791 t
ORIGIN      
        1 cacggtggta tacgcggtca gaacgtcacc ggttgctgaa gaatcaaggg ggggatacgc
       61 ctgctctgca ggcgaaggtg gactgaccat caattgccgg gtgtaaaaac atggaaccct
      121 gagcgaatca cttcacgccc agggttcata ggccgggtgg gcctatcgtt actctaagta
      181 tagcacacat cccgcgccgg tttcagggtt gaaaaaaggg gtgtccggtc atctcaaggg
      241 cttgatgcgc cctgcagagg gacgaaaaaa acaaggggac cggaatgtcc ggtccccttt
      301 tcatgagcgt gatgagtatt atatttcgtt gacagtgatc gcctgctgcg ggcagttctc
      361 cacgcaggtc tggcaattga tgcactcgga catattgatc gggtctgcct tgccgccgtc
      421 cactttgaat acggactgcg ggcacatatt tacgcaggta ccgtcaccgt cgcacttgcc
      481 cttgtcgatc gttaccatgt acatgtggcc tggttcctcc ttctgtgaaa gtatgattcc
      541 gaattccaaa aagcatccca taatacccgc tggggccgat aaaatcaaga aagaaataaa
      601 ggaaaaacgg cccgccctca tgaggggcag gccgcaaaaa ggagcgttcg taagtgccgg
      661 attttattaa tgctctatct ctatcccggc cgtcgcctcg cgcaccgaga tcgcgatctt
      721 gtacagcatg gacaggacca ggaacccgat tgaccagact cccagggtga tcagtgcctc
      781 gggcatcgtc ggccagtagg gatgcacttc ctcgaagggg ttcggcacga aaccgccgat
      841 gaccaggcca aagcccttgt cgatccagag cgagatgaag agcatgacgc aggccgaggc
      901 gaggagccct tccttcttgc gggcctgcgg gaagacgagg atcacgatcg atgcagccgc
      961 aaggatcgtc gacgtccaca tgagcttcac cagctcggtg tggccttcga gaccgacata
     1021 gagatacttg aacggggtgg cgtggcccgg gatgccgctg tagaaggcag tgaagaactc
     1081 caggcccagg aggaagacat tggccaacat ggcgtaggcc acgatcttgc cgaccgcctg
     1141 gatggcctcc tttccgggat cgaacttcga tatcttccgg acgatgagcg acaggatgat
     1201 aagcagcgcc ggaccgccgg caaaggccga ggcgaggaag cgggcagcca tgacggccga
     1261 aagccagaag tggcgcgccg gaaggccggc atacaggaaa gccgtcaccg tgtggatgct
     1321 gacagcccag gggatcgaaa ggtagatcca gggcttgatc cacttggggg ggggcgtgcc
     1381 tttcctgtcg gaactcaagg tgacccagcc gaccagcagg ttgatgcaca ggtagcccac
     1441 caagaccacg gaatcccaga acatgacgga gttcggcgtg gggtgcagaa ccacattcat
     1501 gatcctcatc ggctggccca tgtccacgaa gatgaacagg atgcacatgg tcaccgcgga
     1561 gatcgcgagg aactctccca ggatcacgat cttgccgaat ttcttgaagt cgtgcaggta
     1621 gtacgggatg acgagcatca ccgccgaggc ggcaacgccg acgaggaatg tgaactggcc
     1681 tatgtcgagg ccccatgaga catcacgatg caggccggtg atccccaatc cgtattggaa
     1741 ctgctggagg tagaaataca cccccaccag cgcgagggcg ccgagaaagc acagccaccc
     1801 ccagtatgat tttcctccct caaatgcttt ttcaagcatg ttcgctccct cctatcagat
     1861 agtaaacggc gggtccggtg ccaagctcgg gctttctcct gacggtgagc tgggatgtca
     1921 cgatctttct gacctcggaa ttcgggtctt ctatgtcccc gaagaacatc gcgcccttag
     1981 gagcggcctc gacgcaggcc ggtatcttcc ccgcctggag cctctcggag caaaaggtgc
     2041 atttctccac cacacccttg gtcctgcatg ggtattcctt gttctcgaat tccggcttga
     2101 gcgccttcgc cgcgcgggga tcgatccagt taaagctcct cgaaccgtag gggcaggcgg
     2161 ccatgcagaa acggcacccg atgcagcggt gcatgtcctg catcgtgatc ccgttcgtca
     2221 gcttgaacgt cgccttcgtg gggcagaccc gcacgcaggg cgggttttcg cagtggttgc
     2281 acaggacgag gaagggcatc tccttcacgt gctcgctcat atactggttt tccgcagccg
     2341 gaaacgtgtg ctcgtagggt tccgtccaga tccacttgat ctcgaacttc ttgtccacag
     2401 tgccgtccgg gttccggaaa tccggcacat tgtgcgtatg gtggcaggcg tcggtgacct
     2461 ttttgatgtc ttcctcggtc ttgaacttgc tcatgtccac gaccatcgcc cagcgcttgc
     2521 cgacgagacc ctccttgctc ggctcgatct gcggcggctc aagccccttc tgccagatcg
     2581 tgtgtgtggc cgatgcgacc gacgcgccga accccaggat ggaggagatc ccggcaattt
     2641 tcagaaattt tctcctgtcc atgctcatga tttaacctcc tccttttcct gcggctggat
     2701 atggcagtcc cagcaatagg gcttgaccgc catataattg tggcattcgt cgcagaattt
     2761 tttcttgttc gaatggcatt tcatgcagcc gttctgcagg ctcatgttga acttcttgcc
     2821 gcttgtctgg ctcacgtact gccggtacat gtcgcggacg acggcgtcgc gccagttgtt
     2881 cagcatctgc atgtgttcgg accgcatgta ctccttcgat tcaacgcact ccttctttcc
     2941 gtacagcttc tcccattcct ggatcgcagg ggtgtccgtt ttcggctccg gtttcgcgtt
     3001 caccttcccc agattgttgt agaacgggaa cagggcaaag gccagaaaga ccgccagccc
     3061 gatgatgatc ttccctccat catagagttt cattaggctt taacctcctt ctgtgccttc
     3121 gcagggagcg gctcgccgcg cagatttgtc tccctcggtt tttcgccctt cataatcagg
     3181 gcattggcca caagctcatg caggccgcag acgttgacgc tgggtaccca gtactccatc
     3241 agggccttga gcgttgcccg gtcgatggcg catatgttgg ccagcatatt cacgccgtag
     3301 tgttctgcga cgaacttgac cgcgttggcc cgggggaacc cgcctttcat gcggagttcc
     3361 atgtcctccg aggcgttgag gcccgtgccg ctgccgcaac agaaggtttt ctcccggatc
     3421 gtgtcttccg gcatttcgtg gaagtggttc accacgctct tcagtacgta gcgcggctct
     3481 tccaggaggc ccatgccccg cgacgtgtta caggagtcgt gccacgtcac gatgaggtcg
     3541 tcgttcctgc tcggatcgag gttcagcttg ttgttgcgga tgaggtccgc cgtgaattcg
     3601 gcga
//
LOCUS       DASSLD010000370         3407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1338553, whole genome shotgun sequence.
ACCESSION   DASSLD010000370 DASSLD010000000
VERSION     DASSLD010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338553"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..2822)
                     /locus_tag="VFK23_06665"
     CDS             complement(117..2822)
                     /locus_tag="VFK23_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7318802.1"
                     /translation="MSGQRTIQEQIGALSASLADKLGLAGQDDPAFAALRPLLDQDAE
                     LRFLGIVESFLSLDQSLSRTLLASAPAALGIIHDQAARAAFLRVAADMGRSKWSVMER
                     LFRELPALKGRADAVILGWGSSAARLADKDQDVALEFVKATGAMLMALDDEAFGAWSK
                     NGVEITLKAWKAGREYFRSSAEVIKKIDVCDLDRWAKLGLLLIDKAPDISASYSAHSL
                     LAVGAAAGKAKKAELALQYFKSAPQILGRLTVHDLEEWVSRGLQNVESQADKGKSFFS
                     LQTGSSRTTMESLVKGLELRDIHTVLRDYAQALIGAKIMLRSSSIFYKNLPGLSRFFS
                     VTDGWRIFVPSRIEVFADEEMNFKTYKWVLTHELAHILHGTFDIGASTIRDELAALPD
                     PRQAFRIFEFLEDERVDHLMMNAYPGLSRDRDAILSAFLAREREKGITQHPFLEQIGL
                     RGIGGLTEGVDRPGLSHLVEQALDRVLRPGATSRDALNAAVEIGRTLEGGNSCNLSES
                     KDAGHRLFYRGVIDYDLVENSRVGTVRLVADLVDRFSDRKQPVEAPLVEIALRRIEQE
                     TFIDSEELLWQIQEPARLTELFDQVKAVLEEMEAEKRFRRTVYYDEWDSKIGDYRKDW
                     VRVREMDTPESSSAFYDRTVSEQYGLVSILRRYFGLLRPDRIQRFFREERGDDIDFDA
                     MIESVVERHAGVTPSDRVYIRREKNLRDVSVAFLVDMSYSTGDELPSGKRIIDVEREG
                     LVLMAEALESIGDQWAVYGFSSNYRDKMDFYVIRDFDERSPVRFQQRFDYIKPMAQTR
                     LGAAIRHATKLLARQGSRIRLLILLSDGRPYDVDYGDADYAVEDTRHALWEAKKKGVF
                     SFCITVDKKSRDYLPYMYGETNYTLIDNVETLPIRLPLIYKRLTT"
     gene            complement(2819..>3407)
                     /locus_tag="VFK23_06670"
     CDS             complement(2819..>3407)
                     /locus_tag="VFK23_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ C-terminal domain-containing
                     protein"
                     /protein_id="HET7318803.1"
                     /translation="SDLVGRYLLDGGTTRWQDGPLTLAVRYGGICYLDEVVEARKDTT
                     VVIHPLTDDRRVLPLEKRGQILEATDNFMLALSYNPGYQTVLKDLKQSTKQRFITLDF
                     DYPPASLEQEIVVHESGVDHKTASELRKIAEKFRGLKDRGLDEGVSTRLLIYAGVLIT
                     RGVEPRRACEVALIKPITDDLEIQKTLKEIVSAVL"
BASE COUNT          618 a         1061 c         1012 g          716 t
ORIGIN      
        1 aaaccagcag ctttgaacca cgaaggtcac gaagaccacg aagaaatttc cgttaacctg
       61 taattggctt taccttcgtg cacttcgtgt gtttcgtggt aatttgtgtc gtctctctat
      121 gtcgtcagtc ttttatatat cagcggcagc cgtattggca gcgtttccac gttgtcgatg
      181 agcgtatagt tcgtttcccc gtacatgtac ggaaggtagt cccggctctt cttgtccacc
      241 gtaatgcaga acgagaacac gcctttcttt ttggcttccc agagggcgtg gcgtgtgtcc
      301 tctaccgcgt aatcggcgtc gccgtaatcg acgtcgtagg gccggccgtc ggacagaagg
      361 atgaggagcc ggatacgcga accctgccgg gcgagcagtt ttgtggcatg gcgtatcgcc
      421 gcgccgagcc tggtctgggc catgggcttg atataatcaa accgctgctg gaagcggacc
      481 ggtgaccttt cgtcgaaatc gcgtatcacg tagaagtcca tcttatcccg ataattggag
      541 gaaaagccgt acaccgccca ctggtctccg atactctcca gcgcctctgc catcagtacc
      601 agcccttcac gctcgacgtc gatgatcctc ttgcccgagg gaagttcgtc tccggtggaa
      661 tagctcatgt ccaccaggaa ggctacggac acatcgcgca ggttcttttc ccgccgtatg
      721 tagacccggt ccgacggcgt gacgccggca tgccgctcga ccaccgactc gatcatggcg
      781 tcgaaatcga tatcgtcgcc ccgttcctcg cggaagaacc gctggatgcg gtcgggccgg
      841 agcaggccga aataccgtcg aagaatactc acgagtccgt actgctcgga gaccgtccgg
      901 tcatagaatg cagatgagct ttccggcgtg tccatctcgc gcacccggac ccagtccttc
      961 cggtagtcgc cgatcttaga atcccattca tcgtaataga cggtccggcg gaaccgcttt
     1021 tccgcctcca tttcctcaag cacggccttg acctggtcga agagctcggt caaccgggcg
     1081 ggctcctgta tctgccagag cagttcctcg gagtcgatga aagtttcctg ttcgattcgc
     1141 cggagcgcga tttcgacgag gggagcctcg acgggctgct tccggtccga aaagcggtcg
     1201 accaggtccg cgacgagccg cactgtcccc acgcgggaat tctcgaccag gtcgtagtcg
     1261 atgacgcccc ggtagaacag gcggtggccc gcatccttgc tttcggacag gttacaggag
     1321 tttccgcctt cgagcgtacg gccgatctcg acggctgcat tcagtgcgtc ccgcgatgtg
     1381 gcccccggcc ggagcactct gtccagggcc tgctcgacca ggtgcgacag gccggggcgg
     1441 tctacgcctt cggtaaggcc gccgatgccg cgaaggccga tctgctccag gaacggatgc
     1501 tgggtgatgc ccttctcccg ctcgcgggcg agaaaggcgg acaggatcgc gtcccggtcc
     1561 ctgctgaggc ccggataggc gttcatcatc aggtgatcca cgcgttcatc ctcaagaaac
     1621 tcgaagatcc ggaatgcctg gcgggggtcc gggagcgccg cgagttcatc cctgatcgtg
     1681 gaagcgccga tatcgaatgt gccgtggagg atgtgggcca gttcgtgggt cagcacccac
     1741 ttgtaggtct tgaagttcat ttcctcgtcg gcgaagacct cgatcctgct gggcacgaag
     1801 atcctccatc cgtcggtgac cgagaaaaac cggctcaacc cgggaaggtt cttgtaaaat
     1861 attgatgacg aacgcagcat gatcttcgca ccaatcagcg cctgggcata atcccgcagc
     1921 accgtatgga tgtccctcag ttccagccct ttgaccaggc tttccatggt tgtgcggctg
     1981 gatcccgtct ggagcgagaa gaaggacttg cccttgtcgg cctggctctc cacgttttgc
     2041 aggccccggc tgacccattc ctcgaggtca tgtacggtca gccggcccaa gatctgcggg
     2101 gccgacttga aatactggag cgcaagctcc gcctttttcg ccttgcccgc cgctgcgccg
     2161 accgcgagca gactgtgcgc cgagtacgat gccgagatgt ccggcgcctt gtcgatcagg
     2221 agcagcccca gcttcgccca ccggtccagg tcgcagacat cgatcttctt gatcacctct
     2281 gcgctggaac ggaaatactc gcgtcccgct ttccaggcct tgagcgttat ttcgacgccg
     2341 ttcttcgacc acgcgccgaa ggcctcgtcg tcgagggcca tgagcatcgc gccggtcgcc
     2401 tttacgaact cgagagccac gtcctggtcc ttgtccgcca gcctggccgc agaagatccc
     2461 catccaagga tcaccgcatc ggccctgccc ttcagggcgg gtagttcgcg gaacagtctc
     2521 tccatgacgc tccatttgga tctgcccatg tccgcggcga ccctgaggaa ggcagcccgt
     2581 gcggcctggt catggatgat gccgagagcg gccggggccg acgcaaggag cgttcgcgag
     2641 agcgactggt ccagggacag gaaagactcg acgatgccca ggaaccggag ctcggcatcc
     2701 tggtcgagaa ggggccgcag ggctgcaaag gcagggtcgt cctgtcccgc gaggccgagt
     2761 ttgtcggcaa gggacgccga gagggccccg atctgctcct gtatggttcg ctgcccgctc
     2821 ataggactgc tgacacgatc tccttcaatg ttttctggat ctccaggtcg tcggtaatgg
     2881 gtttgatgag ggcaacctcg caggcccgcc tgggctccac cccgcgcgta atcaataccc
     2941 cggcatagat gagaagccgc gtgctgaccc cttcgtccag tccccggtcc ttcagcccgc
     3001 ggaacttctc cgcgatcttc ctcagttccg acgcggtctt gtgatcgact ccgctctcgt
     3061 gtactacaat ctcctgctcc aggcttgcag gcggataatc gaagtccagg gtgatgaaac
     3121 gctgcttggt gctctgcttc aggtccttga gaactgtctg gtagccgggg ttatacgaga
     3181 gcgccagcat gaagttatcc gtcgcttcca ggatctggcc ccgtttttcc agaggcagca
     3241 cccggcggtc gtcggtcagg gggtggatca cgacggtggt gtccttccgc gcctccacga
     3301 cctcgtcaag gtagcagatg ccgccgtacc ggacggcaag agtgaggggg ccgtcctgcc
     3361 agcgtgtcgt gcctccgtcg aggagatatc tgcccaccag gtcgctc
//
LOCUS       DASSLD010000371         2888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_1649,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000371 DASSLD010000000
VERSION     DASSLD010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..211
                     /locus_tag="VFK23_06675"
     tRNA            135..211
                     /locus_tag="VFK23_06675"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:169..171,aa:Arg,seq:acg)
     gene            461..934
                     /gene="tadA"
                     /locus_tag="VFK23_06680"
     CDS             461..934
                     /gene="tadA"
                     /locus_tag="VFK23_06680"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016131046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HET7318804.1"
                     /translation="MTTYPNAKTDMDFMRLALAEAEKAAAHGETPVGAVLVIEDEVIA
                     AARNMRETWQDPAAHAELLVIRVASARLGRWRLPDATVYVTLEPCLMCAGALVLARIG
                     RLVYGCRDPKAGALGSVYDVVRDGRLNHTYRITPGVLEAESREVLTNFFERLRTK"
     gene            1035..1124
                     /locus_tag="VFK23_06685"
     tRNA            1035..1124
                     /locus_tag="VFK23_06685"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1069..1071,aa:Ser,seq:cga)
     gene            1242..1329
                     /locus_tag="VFK23_06690"
     tRNA            1242..1329
                     /locus_tag="VFK23_06690"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1276..1278,aa:Ser,seq:gga)
     gene            1442..1541
                     /gene="ffs"
                     /locus_tag="VFK23_06695"
     ncRNA           1442..1541
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFK23_06695"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1572..>2888
                     /gene="dnaX"
                     /locus_tag="VFK23_06700"
     CDS             1572..>2888
                     /gene="dnaX"
                     /locus_tag="VFK23_06700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET7318805.1"
                     /translation="MSYQVLARKWRPQVFEDVVGQGHITRTLQNAITSGRLAHAFLFS
                     GPRGVGKTTTARILAKALNCAEGPTPTPCGKCNSCVETAAGTSVDVIEIDGASNRGIE
                     HIRELREAVKYAPVGGKNKVYVIDEVHMLTNEAFNALLKTLEEPPPHVIFIFATTEPQ
                     KIPATIHSRCQRYGFKRVSLREIIGRLRTIAAAEGITISDRGLAMIARAAEGSMRDSQ
                     SLLDQAVSYSGMSIRDEDLQATLGAVAQERLLQFAAGLLERNAAGLLKSIDDLLEQGQ
                     DMRQFLSGVVEHFRNLAVVKIAADPAGIIELPEADVEALKKQAAGADAGQLLMLFDSL
                     SRTLDEMRWSPHQRFTLEVGLIKVCSLAPLEPLADVLGRMKELEARLAAGKTMQTPAP
                     SPHGISEQRASYVPAPKTAAPQHRTVSTSGEEPGDVWGRILSVLKSR"
BASE COUNT          596 a          861 c          877 g          554 t
ORIGIN      
        1 aaattcatcc cttccctcac gaaaagatcg cgattacgct cgtcggcggt gtgtccaagt
       61 aacaggcatc cccgggtggt gcttttatgc gtccctgtcc cggtgcgctt gctgcgcatc
      121 ggggcgtttt ctttgcgccc atagctcagt tggatagagc gacggactac gaatccgtag
      181 gccagaggtt cgaatcctct tgggcgcgcc aatattctgg ctaacaagat gcacgttgca
      241 gcagcgatat aaacggacca agaagagaac ctctgacctc aggggtttca ggggaaggat
      301 tcccctgagt cttgggggag ataccagagg gggcgagccc cctttgggga gccctttacg
      361 ggcgacggcc agaggttcga atcctcttgg gcgcgccaat ttatatttac tgtcgaatgc
      421 gaaccacgta accgaaacgg gcctcgcggc cgtcataatt atgaccacat acccgaatgc
      481 aaagaccgac atggacttca tgcgccttgc cctggcggag gcggaaaagg ccgcggccca
      541 cggcgagacg ccggtcggcg ctgtgctcgt catcgaagat gaggtcatag ccgcggcccg
      601 taacatgcgg gagacttggc aggaccccgc tgcccacgca gaactgctgg tcattcgtgt
      661 ggcatcggcc cggctcggca ggtggcggct tccggacgcg acggtctatg tgaccctgga
      721 gccctgcctc atgtgcgccg gagcgctggt gctggcgagg atcggcaggt tggtctacgg
      781 ctgcagggac cccaaggcgg gcgcgctcgg ctcggtgtac gacgtggtac gggacggcag
      841 actgaaccat acgtatcgaa tcacgccggg tgtgctcgaa gcggagagcc gcgaggttct
      901 gacaaatttt tttgagaggc tgcggactaa atagttcgga gttcgtagtt gggagttcgg
      961 gatcacaaag gcaaagacag ctgacagccg atcctctccg cgctccgaac tcaacactcc
     1021 gaactgtagt aaacggagag gtggccgagt ggctgaaggc ggcagtctcg aaaactgttg
     1081 tgggggtaac tccaccccgg gttcaaatcc cggcctctcc gccatgttct aaaaaattag
     1141 ttcggagttc gtagttggga gttcggagtc acaaaggcaa gaacggttga ctttcgatta
     1201 tctccgaact ccgaactcaa aacttcaaac tgtagtcaac cggagaggtg gccgagcggc
     1261 tgaaggcgca cgcttggaaa gcgtgtttag ggaaacctaa cgtgggttca aatcccaccc
     1321 tctccgccat attaaaaaat aagttcggag ttcttagttc ggagtaaaac agcttcaatc
     1381 gtttttttat gtctccgaac tcacaactcc gaactctgaa ctgaagtaat cgcgctcatg
     1441 agcccgggtc ctgcgcaacc gcggtcccgt gaaccccgtc aggtccggaa ggaagcagcg
     1501 gtaagcgggt tccgggtgtg ccgcaggggt gcccgggttt atgagccttt tttattttga
     1561 gggtatttac gatgagctac caggtcctgg cgcgaaaatg gagaccccag gtttttgaag
     1621 acgtggtggg ccaggggcac atcacccgga cgctccagaa tgcaatcaca tcggggcggc
     1681 tcgctcacgc ctttctgttt tccggcccgc gcggcgtggg aaagacgacg acggcgcgca
     1741 tcctcgcgaa agcgctgaac tgcgccgagg gcccgacgcc gacgccctgc ggcaaatgca
     1801 actcctgcgt cgagactgcg gccggcacgt cggtcgacgt catcgagatc gacggcgctt
     1861 cgaaccgcgg catcgagcat atccgggagc ttcgcgaggc cgtcaagtac gcccccgtgg
     1921 gcggcaagaa caaggtctac gtcatcgacg aagtccacat gctgacgaac gaggccttca
     1981 atgcgctcct gaagaccctc gaggagccgc cgccgcacgt cattttcatc ttcgccacca
     2041 cggagccgca aaagattccc gcgacgatcc actcccgctg ccagcgctac ggtttcaagc
     2101 gggtttctct ccgcgagatc atcggccgtc tccgcacgat agccgccgcc gagggtatca
     2161 cgatttccga ccggggtctc gccatgatcg cgcgcgctgc ggaaggcagc atgcgcgatt
     2221 cccagagcct tctggaccag gccgtatcgt acagcggcat gagcatacgc gacgaagatc
     2281 ttcaggcgac cctgggcgcc gttgcccagg agaggctcct gcagttcgcc gccggccttc
     2341 tcgagcgcaa tgccgccggc ctcctgaaat cgatcgatga cctcttggag cagggtcagg
     2401 acatgcgcca gtttttgtcc ggtgtcgtcg agcacttcag gaacctcgcg gtcgtcaaga
     2461 tcgcggccga tccggccggc atcatcgagc ttcccgaagc ggacgtcgag gccctcaaaa
     2521 agcaggcggc gggtgcggat gccggacaac tgctcatgct cttcgacagc ctgtccagga
     2581 cgcttgacga gatgcgctgg tctccgcacc agcgttttac gctcgaggtc ggactgatca
     2641 aggtctgcag tctggcgccg ctcgagcccc tcgccgacgt cctcggccgc atgaaggaac
     2701 tcgaagcgcg tcttgcagcg ggcaagacta tgcagacccc cgctccctca cctcacggga
     2761 taagcgaaca gcgcgcctcc tacgtccctg ctccgaaaac ggcagcaccc cagcaccgca
     2821 ccgtttcgac aagcggagag gagcctggag atgtctgggg caggatattg tccgtgctga
     2881 aatcacga
//
LOCUS       DASSLD010000372         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_437804,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000372 DASSLD010000000
VERSION     DASSLD010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437804"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFK23_06705"
     CDS             <1..435
                     /locus_tag="VFK23_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318806.1"
                     /translation="PAAAPFDVAKFAGIFAAIGLAIGAIGTAIAALVTGLLRLAWWQV
                     PLAFLGIVLIISGPSMALAWFKLRQRNLGPLLDAGGWAVNARARINIPFGTQLTSIAQ
                     IPEGAERSLFDPYKEKKGPLPFIIAVAVVLGLVAAYFFLFKR"
     gene            517..777
                     /locus_tag="VFK23_06710"
     CDS             517..777
                     /locus_tag="VFK23_06710"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET7318807.1"
                     /translation="MSINKIKPDADRALVNKVIPEHREWAKRQLAAGTLVQAGKWGDR
                     GGMIVIQADSMAEAEKIVCQDPFVVADLIIFETAELHPAKAF"
     gene            1180..1818
                     /locus_tag="VFK23_06715"
     CDS             1180..1818
                     /locus_tag="VFK23_06715"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET7318808.1"
                     /translation="MFTGIVEEMGSVKSLRRETGAARLTISASTVLDGTALGESICVN
                     GVCLTVVDLNKNEFSADVAVETLKVTNLGELTIGAKVNLERALQLSARVGGHLVSGHV
                     DAVGRIREKRVEGNGWRIFVDAPDEVLRYIIKKGSIAVDGISLTVADVDKTGFSIAMI
                     PHTARLTTLGFKAAGDSVNLEADIIGKYVERLLSGRVEGGVSLELLKKTGFA"
     gene            1902..>2012
                     /locus_tag="VFK23_06720"
     CDS             1902..>2012
                     /locus_tag="VFK23_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HET7318809.1"
                     /translation="MKFNTIEEAIEDIKKGKMVILVDDEDRENEGDLTMAA"
BASE COUNT          453 a          551 c          576 g          432 t
ORIGIN      
        1 ccggcggctg cccccttcga cgtggccaaa tttgccggca tctttgcggc gatcggcctg
       61 gccatcggcg ccatcggcac ggcgatcgcg gctttagtga cgggcctgct gcgtctcgcc
      121 tggtggcagg tcccgctcgc ctttttgggg atagtcctga taatctcggg tccctccatg
      181 gctctcgcgt ggttcaagct gcgccagaga aacctcggcc ccctgctcga cgccggggga
      241 tgggcggtca acgcccgtgc caggataaac attcccttcg gcacacagct aacatcgatc
      301 gcgcaaatcc ccgagggtgc ggaacgctcg cttttcgatc cgtacaagga aaagaaaggg
      361 ccgcttccgt tcatcattgc agtagcggtc gtcctcggac ttgtcgccgc ctattttttc
      421 ctgtttaagc gataaccggg tcagctcttc acggctcatg cggagtattg agagtaaccg
      481 gtaaaaaaaa tacgaccaga ggtcgccgtg tatttcatgt ccattaacaa gatcaaaccc
      541 gacgcggacc gggcgctcgt gaacaaggtc attccagaac accgggagtg ggcaaaacga
      601 cagctcgctg cgggaacgct cgttcaggcc gggaagtggg gcgaccgggg aggcatgatc
      661 gtcatccagg cagacagcat ggctgaggcg gagaagatcg tttgtcagga tccgttcgtt
      721 gttgcggacc tcataatctt cgagacagca gaactccatc cggcaaaggc gttttaatga
      781 gtatcatact actcaggtcg tatgattcga cgttccagac gatcatggtc cgctttgacc
      841 cattcctacc ccagccctcc cctcagcgtg gcccgcttca tcggccatga aggggagggt
      901 gttagagtgc tccgaactcc gaactctcta ctccgaacta cgtcctctcc tctctgaacc
      961 gctgttctaa acccttcttt ctccacaata ttcttgacaa taacgccggt cgcagttatt
     1021 ataacgagtc tccgtcttca taaacacttg caggttatta ctacgcaatt cttcaaagaa
     1081 aaggcttttt aatcaaattg tttcctcagt gatgcgtacc ctgttcctgg tgcgggcagg
     1141 cgaatattct ccgaacataa agaggtcgtt tattatatca tgttcaccgg gatcgtggag
     1201 gagatgggaa gcgtgaagtc tctcaggcgc gaaacgggtg ctgcccggct cacgatttcc
     1261 gccagcacgg tcctcgacgg tactgccctg ggcgaaagca tctgcgtaaa cggggtctgt
     1321 cttaccgttg tcgacctgaa taaaaacgag ttctccgctg atgtggcggt tgaaacgctc
     1381 aaagtcacga acctgggcga acttacgatc ggcgcgaaag tgaacctgga acgggcgctc
     1441 cagctttccg cacgggtcgg cggccacctc gtgagcgggc acgttgacgc ggtcggccgg
     1501 atccgcgaga agcgcgtcga ggggaacggc tggaggatat tcgtcgatgc gccggacgag
     1561 gtccttcggt acatcatcaa aaaaggttcc atcgccgtcg acggcatcag cctgacggtg
     1621 gcggacgtag acaagacggg tttttccatc gccatgatcc ctcatacggc cagattgacc
     1681 accctcggtt tcaaggcagc gggcgacagc gtgaacctgg aggcggacat catcggcaag
     1741 tatgtggagc ggcttttgtc cggaagggtc gaaggcggcg tgagcctgga actgctgaag
     1801 aagacagggt tcgcatgaat tcaattctgt ttcggatggc cgatcaagcg ggccaccctg
     1861 atgcggaata cggaatttgc gaggaaaaag aaaggcattt tatgaagttc aataccattg
     1921 aagaagccat agaagacatc aaaaagggga agatggtcat actcgtcgac gacgaggacc
     1981 gggaaaacga aggcgacctg accatggcag cc
//
LOCUS       DASSLD010000373         2895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_555824,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000373 DASSLD010000000
VERSION     DASSLD010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555824"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFK23_06725"
     CDS             <1..414
                     /locus_tag="VFK23_06725"
                     /inference="COORDINATES: protein motif:HMM:NF018580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HET7318810.1"
                     /translation="EEKAAPAKPTEGWTLHIDAKKHFPYLPDMIAHHYCKPVSGGLTE
                     CQIYDGDKPDARLVAVEVIVDKATWEKFPREEKALWHHHKVEIPKVEAVLPDVSPEES
                     AKTLKSLEETYGKVYVLWDPAKKEPVGKPQVSVLH"
     gene            complement(480..920)
                     /locus_tag="VFK23_06730"
     CDS             complement(480..920)
                     /locus_tag="VFK23_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HET7318811.1"
                     /translation="MNTKNRRFSKKKIRVLLAKAGLDGHDRGIKVIAMALRDAGMEVI
                     YLGLRRTPEEIVSAALEEDVDVIGISLLSGAHTVLFPAVLSLMKKKKLRGMILIGGGI
                     IPDEDQVSLRKKGVAAIFTPGAETGEIVRFIQEAVGRKAESKSI"
     gene            complement(892..2565)
                     /locus_tag="VFK23_06735"
     CDS             complement(892..2565)
                     /locus_tag="VFK23_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET7318812.1"
                     /translation="MKNGTKKIKAEKRRWKQKVLKPSLKKLPERLPAFTTVSNLPIDE
                     PVYTPADIAGLVHAKDLGFPGEYPYTRGIYPSMYRSRLWTIRQFSGYGAAEETNRRFK
                     YLLKEGETGLSVAFDLPTLMGYDSDHPLSEGEVGRCGVAVDSLADMEVLFRDIPLDRI
                     TTSMTINAPAAVLFAMYLALAEKKGVPQARLGGTIQNDILKEYIAQKEWIYPPKPSLR
                     LITDTIAYCVRKVPKWHPISISGYHIREAGSSAVQELAFTLADGLTYVEACVRAGMKI
                     DDFAGRLSFFFNSHNDFFEEIAKFRAARRIWAREITKRYRPKDPQSVQMRFHTQTAGC
                     SLTAQQPYNNLIRTTVQALAAVLGGTQSLHTNSMDETLALPTERAVTMALRTQQVIAH
                     ESGVANVIDPLGGSYFVEALTNKIEKDVYDYFKRIDDLGGMIEAISQGFPQREIARSA
                     QWYQQAVEKKEKIVVGLNEFTEAGKPIEVLKIGPEIEKRQVRRLQALRRKRDKGRWAS
                     SLNRLEAAAAGRENLVPFIIDAVKAYATVGEICGVLKGVFGEYKEPALF"
BASE COUNT          576 a          848 c          823 g          648 t
ORIGIN      
        1 gaggaaaagg ctgcgccggc aaagccgacg gaaggctgga cgctccatat cgatgcaaaa
       61 aagcattttc cgtacttgcc ggacatgatc gcccatcatt actgcaaacc ggtatcggga
      121 ggactcaccg aatgccagat ctatgacggc gacaaaccgg acgccagact cgtcgcggtc
      181 gaagtaatcg tcgataaggc gacatgggag aaattcccca gggaagaaaa ggcgctctgg
      241 catcatcaca aggtcgagat cccgaaggtc gaggctgttc tgcctgatgt ttcccctgag
      301 gaatcggcaa agacgttaaa gagtctggag gagacgtacg gtaaagtgta tgtcctctgg
      361 gaccctgcga agaaagagcc ggtcggaaag ccgcaggttt cggtactgca ttaaataaat
      421 tgccgcaaag gcacaagatg cgcatgggat attttttgtg acttttgtgc ctcttgcggc
      481 taaatagatt ttgattccgc tttcctgccc accgcctcct gtatgaaccg cacgatctcc
      541 ccggtctccg ccccgggggt aaagatcgcg gcgacccctt tttttctgag cgacacctga
      601 tcttcgtcgg gaatgatgcc gcccccgatc agaatcatgc cgcgcagctt ctttttcttc
      661 atcagcgaca ggaccgcggg aaagaggacg gtatgcgcgc cggagagaag gcttatgccg
      721 atcacgtcga cgtcctcctc gagcgcggcg gagacgattt cctccggcgt ccgtctgagt
      781 ccgagataga tgacctccat gccagcgtcg cgaagggcca tggcaatgac cttgatcccc
      841 cggtcgtggc cgtccagccc ggccttggcg aggaggaccc gtatcttttt cttagaaaag
      901 cgccggttct ttgtattcac cgaagacccc cttcaggacg ccgcagattt ctcccaccgt
      961 cgcatatgcc ttgacggcat cgatgatgaa cggcacgaga ttctccctgc ctgccgcggc
     1021 ggcttcgagc ctgttgagcg aagacgccca gcggcccttg tcgcgcttcc gccggagtgc
     1081 ctggagccgc cggacctgtc ttttttcgat ctcgggtcct atcttcagga cctcgatcgg
     1141 tttcccggct tccgtgaatt cgttcaggcc gaccacgatc ttttcctttt tctcgacggc
     1201 ctgctgatac cactgggcgc tccgcgcgat ctcgcgctgc gggaatccct ggctgatcgc
     1261 ctcgatcatc ccgcccagat cgtcgattcg tttgaagtag tcgtacacat ccttctcaat
     1321 cttgttcgtg agcgcctcga cgaagtagga gccgcctaag ggatcgatga cgttcgcgac
     1381 cccgctctca tgggcgatca cctgctgagt ccggagcgcc atggtcaccg cccgttccgt
     1441 gggaagcgcg agtgtctcgt ccatggaatt ggtatggagc gactgggtcc ccccgagcac
     1501 ggcggcaagg gcctgcacgg tcgtccgtat gaggttgttg tagggctgct gcgccgtgag
     1561 agaacagccc gccgtctgcg tgtgaaaacg catctggact gactgcgggt ccttcggccg
     1621 gtatcgcttc gtgatttccc gcgcccagat cctgcgggcc gcgcggaact tcgcgatctc
     1681 ttcgaagaag tcgttgtggg aattgaagaa gaacgagagc ctgcccgcga agtcatcgat
     1741 cttcatgccg gccctgacgc aggcctccac ataggtgagc ccgtcggcaa gggtgaaggc
     1801 gagctcctgc acggcgctcg aacctgcctc gcggatatgg tagccgctga tgctgatcgg
     1861 atgccatttc gggactttcc tgacgcagta cgcgatcgtg tccgtgatga gccggagcga
     1921 gggcttcggc ggatagatcc actccttctg ggcgatgtat tccttcagga tgtcgttctg
     1981 aatggtcccg ccgagcctgg cctgcggcac ccccttcttc tccgccaggg cgagatacat
     2041 ggcgaacagg accgcggccg gcgcgttgat ggtcattgag gtcgtgatcc tgtccagggg
     2101 aatgtccctg aacaagactt ccatgtcggc aagggaatcg acggccacgc cgcatctgcc
     2161 gacctctccc tcagacaacg gatggtccga atcatagccc atcagggtcg gtagatcgaa
     2221 agccacggag aggcccgttt caccttcttt caggaggtac ttgaacctcc tgttcgtctc
     2281 ctcagccgca ccgtatcccg agaactggcg gatggtccag aggcggctcc ggtacatgga
     2341 gggatagatg ccccgggtgt aggggtactc gccgggaaag ccgaggtcct tcgcgtgaac
     2401 aagcccggcg atatcggccg gcgtatacac cggctcgtcg atcgggagat tggacacggt
     2461 ggtaaaggcg ggaagccgct ccggaagctt tttcaggctc ggtttcagca ctttctgctt
     2521 ccagcgtctt ttttcggctt tgatcttttt cgtcccgttt ttcatgttcc cgtcctcaca
     2581 acattcttta gtacgacttc gccaaagatc aataaaacaa aaccaacatc cttgaaccgc
     2641 caaggcgcga aggttttgcc cgtcttaaat cgagaattgg aatttttcct ttgcaacctt
     2701 ggggccttcg cggtgaactc ttttagctac ggataaaaaa atgttttcaa cgctacctca
     2761 gttccgccct gagcatcctg agctcccgtt catccttcga caagctcagt acaagcggaa
     2821 tgtcgaagga cgaaagatga acggggactt cttcaacagc ctcttagtgg tccgattgca
     2881 ccggactgtg ttaca
//
LOCUS       DASSLD010000374         9019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_264724,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000374 DASSLD010000000
VERSION     DASSLD010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264724"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..2200
                     /locus_tag="VFK23_06740"
     CDS             233..2200
                     /locus_tag="VFK23_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET7318813.1"
                     /translation="MKTFPLTIFLPAAGLGERLRPITNHLPKPLLPVLGRPLIDIILG
                     RITAVCSGRIGINLHYKPEMIRDWVGRSPYADRVTFFPEDPILGTGGALKNAEDFLAN
                     GPFLVHNADILLDIDFTRLIETHLSSGNIATLATHRLPRLSNVVIDENDLVIDVENPG
                     DSRPDPDRVARKVAYTGIAVYSPEILQFLPPGVSHATVAWLAASKAGRRVQALDFTGA
                     PWSDIGTPASYATSVLDALRENGETVYLSPEARCGKIGMDGYVVLEGGSEVLDKARMR
                     NCIVLPGAMVSGRHENRIIGPDYAVDLDETQMQPSLHAREQKKVVLSDPLFITFFGMA
                     GLPERSEEEVQADAILIGLGGSDRRYYRVRNDGKTAVLMECRQEDPDYERHLLYTRFF
                     EAHSVPVPGILETDETGRCALFEDLGDTSLYSYLKLPRDTEHVEDIYQSVLDILVKMH
                     SRITRHIDECPPLKTRVFDYDHLRWETAYFLERFVIGLKETALRDKSALEEEFHRLAQ
                     KVDAYRKVAIHRDFQCQNIMITAGGMPRLIDFQGARMAPAAYDVASILWDPYCRIADG
                     LREALLDYYVEEMRKQRAGFDEREFSDSLIPCRLQRHMQALGAYGFLSAVKGKKYFLK
                     HVPEALRLLRGDAEAVKSEYPELYKLVKSLS"
     gene            complement(2346..3971)
                     /locus_tag="VFK23_06745"
     CDS             complement(2346..3971)
                     /locus_tag="VFK23_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7318814.1"
                     /translation="MFGGKIAEDPETSIQQFRAIYESSPIGIEIYDAGGELVELNAAC
                     REIFGIDDLSAVKGFRLFEDPNLSDRHKQDLREGRPVQQEIVFDFEKVRKYNLYPTSK
                     RGAIHLDLFISPLVSSGGEAVSGYVVHVRDITKRRHAEEMLLEQKRFAENVVDNSAVA
                     TFVLSPDHRVVLWNKACEELTGFPAAEMIGTSNHWSPFYDRQRPTLADLVIDKEHESL
                     GALYGKFRRSELVPDGVHAEGWYPDMNGKDRYIVFDAAPIYDARQRLIVAIETLQDMT
                     EFKRTGEELEKKTRELTRSNAELERFAHAASHDLKAPLFSIAGFAEVLEEKYGDRLDI
                     NGRKFLSYIMEGTDRMKRLIEDLLAYARVTTKARPLAPVDCAAVVEAAVSNLRSAVGE
                     SGARVTASGLPVVSGDETQLLQLFQNLIGNAINYRSTAPPEIRISAHPLSGAPEEKAG
                     EAGRVPDAGDGRSCAGGGWLFSVTDNGIGIERRHFEKIFQLFQRVHPEDSAYPGTGIG
                     LALCDKIVERHGGRIWVESEPGKGATFYFTIPG"
     gene            complement(4092..4328)
                     /locus_tag="VFK23_06750"
     CDS             complement(4092..4328)
                     /locus_tag="VFK23_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318815.1"
                     /translation="MGKNIEDMTIDELKAELKRLKDNLCDLEDLHAFTFGKTSVHIGA
                     EKAQNLTAEYEDECRLFNVQIAAVEKALKAKGVE"
     gene            complement(4439..5857)
                     /locus_tag="VFK23_06755"
     CDS             complement(4439..5857)
                     /locus_tag="VFK23_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7318816.1"
                     /translation="MDSLVIKYLSQLVSLTPRPVYLVGGSVRDLLAGGREIRDIDLLM
                     PTGSEETARVFADAIGGSFFFLDEERRISRVVKQDDGDFFQFDFMNFEGPDLPADLGR
                     RDFTVNAMALDLREFIARRSIEGLIDPFNGRSDVRDRVIRVVRPAALDEDPLRLLRAV
                     RFAATLGYTIEAETARHIRQRATAIAKPAPERVRDELFLILSQRDAASHLLLLDSLGL
                     LSPLLPELEPLRAFTPGMYRAHDVLTHAVKTAGCVDGVLDDLAALSPAHAAAVVDHLS
                     GQIEHGVPRIAALRFACLLHDMAKPDTLSESEGHVRFHGHDNLGADKAVFLCRRLRLS
                     RNTEALVAKTIKYHMRLFNLSSAGGPSRNAMYRYCRDLGDALPESLILSQADARATYE
                     VMPKEKFADTERPMAQVLDYYYTKFLKAEARPLVSGDDLIKLGLAPGPRFREILEEIK
                     EKQAEGAIRSREEALDYLKTIM"
     gene            6065..6781
                     /locus_tag="VFK23_06760"
     CDS             6065..6781
                     /locus_tag="VFK23_06760"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7318817.1"
                     /translation="MFPIKDTVPRRTFPLATLLIIVANGIVFFFELSMSERYQNAFIE
                     LFGLVPARYTHPLWATVMGFPLDSYWPFLTNMFIHGGWLHFIANMWTLFLFGDNVEDR
                     LGRLRFLLFYLAAGIAANLIHFFVFHDSTMPVIGASGAIAGVMAAYLRLFPRARIITL
                     IPVLFFPFFFDVPAVLFMAIWFFTQLLSGAASLAITESAGGIAWWAHIGGFALGFLFI
                     KPLCKSRSDACNPGEDFRSV"
     gene            complement(7037..7567)
                     /locus_tag="VFK23_06765"
     CDS             complement(7037..7567)
                     /locus_tag="VFK23_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7318818.1"
                     /translation="MAKSLLRWDPFNLMRERAPYFNELRELQADMGRLFDQFLGKEIS
                     TADLGHGEWMPLIESYKKGNDLVFRCELPGIDPKDVEVSFDENTHQLVIKGSRKADKD
                     VKDEDYFYRGFAYGCFERRFTLPEGIKADQVKAKCHNGILEITTPLPAISKAKKIEIE
                     APKTAAEGEVMMKRAA"
     gene            complement(7724..>9019)
                     /locus_tag="VFK23_06770"
     CDS             complement(7724..>9019)
                     /locus_tag="VFK23_06770"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon-insertion domain-containing protein"
                     /protein_id="HET7318819.1"
                     /translation="KIEDLYELYGFGTGGIKPEPIPVSLKIIMLGSPWLYYLLYYYDE
                     DFRDIFKVKSDFDTRTASSSDEKMRYANFIANMVKNDGLLPFDRSAVAGVVDYAARLA
                     EKRDKLSLRFSDLTDLVREASYWAQRAGSGAVLAEHVDKALDEKVYRSNLIDERMQEL
                     FTEGTIMVDVEGSVVAQVNGLSVYEIGDFAFGKPSRITSRVYLGKSGVIDIEREAKLA
                     GRIYNKAVLILSSYLAGSYAHDKTFSVSATLAFEQSYGEVEGDSASAAELIALLSGIA
                     ETPVRQNIAITGSISQKGEIQPIGGVNEKIEGFFSVCKNRGLTGDQGVIIPQLNVKNL
                     MLKKEVVGAVREGKFRVYAVRTVNEALEILTGLPAGERRTDGTWPEGTFSFLVDKRLR
                     EMAKKLKSDDKGEEKKEKKEENNSETAPKKEPPPEAESR"
BASE COUNT         1661 a         2769 c         2584 g         2005 t
ORIGIN      
        1 gtgatatgca ataaatatta ctttcgataa aaacgcctgt tcaaaaaggc gaagattatt
       61 ttttcatatt gaaatgcact cccatctctg ttaatcttta ctgtatgtcg taccactgaa
      121 ctgagtttac acccatgaac gaaatcggcg tttctcggct gatcgataat cacccccacc
      181 tataattcct ctcccatcaa gggcgaggag ctttggagaa gagcatcgtc agatgaaaac
      241 gttccctctt accatcttcc tccccgccgc cggtctcggc gagcggctga ggccgatcac
      301 gaaccacctg cccaagccgc ttctcccggt cctcggcagg cctctgatcg acatcattct
      361 cggcaggata accgcggtct gctccggcag aatcgggatc aacctccact acaaacccga
      421 gatgatccgc gactgggtcg gccgatctcc ctatgcggat cgcgtgacgt tcttccccga
      481 ggacccgatc ctcggcacgg gcggagcgct caagaacgcc gaggactttc tcgcgaacgg
      541 cccctttctc gtccataacg cggacatcct gctcgacatc gatttcacga ggctgatcga
      601 gacccacctc tcctcgggca acatcgccac cctggcgacc caccggcttc cgcggctcag
      661 taatgtcgtc atcgacgaaa acgatctcgt gatcgatgtg gaaaatccag gcgactcccg
      721 gccggacccg gacagggtcg cccggaaggt agcttacacc ggcatcgctg tctactctcc
      781 cgagatcctg cagttccttc ccccaggcgt ttcgcacgcc accgtcgcgt ggctcgccgc
      841 atcaaaagcg gggcgccgcg tgcaggccct cgattttacc ggggccccct ggagcgacat
      901 cggaacgcca gcctcctatg ctacaagcgt gctcgacgcc ctcagggaaa acggggagac
      961 ggtctacctc tcgccggaag cccggtgcgg gaagatcggc atggacggct atgtggtgct
     1021 ggaaggggga agcgaggtcc tggataaggc gaggatgaga aactgcatcg tcctgcccgg
     1081 ggcaatggtc tccggcaggc acgagaacag gatcatcggg ccggactatg ccgtcgatct
     1141 tgatgaaacg cagatgcagc cgtccctcca tgcgcgggag cagaagaagg tcgtcctttc
     1201 ggacccgctc tttatcacct tcttcggcat ggcggggctg cccgagaggt ccgaagaaga
     1261 ggtccaggcc gatgctatcc tgatcggcct cggcgggtcg gaccgccgct attaccgcgt
     1321 ccggaatgac gggaagacgg cggtgctcat ggagtgcagg caggaggacc ccgattacga
     1381 gcggcacctc ctgtacaccc ggttcttcga ggcccactcc gtgcccgtcc ccggcatcct
     1441 cgagaccgac gagaccggcc gctgcgccct cttcgaagac ctgggcgaca ccagcctcta
     1501 ctcctacttg aagctcccgc gcgataccga gcacgtcgag gacatctacc agagcgttct
     1561 cgacattctg gtgaagatgc actcgcgcat cacccggcac atcgacgagt gtccgccgct
     1621 caagacgcgg gttttcgatt acgaccacct gcggtgggag accgcctatt ttctcgaacg
     1681 gttcgtgatc gggctgaagg agacggcctt gcgcgataaa tcagctctcg aagaggagtt
     1741 ccaccgtctt gcgcagaagg tggatgcgta ccgcaaggtc gccatccacc gcgacttcca
     1801 gtgccagaac atcatgatca ccgccggggg catgccgcgg ctcatcgact tccagggcgc
     1861 gcgtatggcg cccgccgcct acgacgtggc ttccatcctc tgggatccct actgccggat
     1921 cgccgacggt ttgcgcgaag cgcttcttga ttactatgtt gaagaaatgc ggaaacagcg
     1981 ggcaggtttc gacgaacggg aattttcaga cagcctcatc ccttgccggc tccagcgcca
     2041 catgcaggcg ctgggggcct acgggttcct gtcggcggtg aaagggaaaa aatatttttt
     2101 gaagcatgtg cccgaggcgc ttcgtctgct caggggggat gctgaagcgg tgaaaagtga
     2161 gtatccggag ttgtataaac tcgtgaaaag cctgtcgtag tgcaggagtc ccctattttt
     2221 gttgccgttc cgggtttggc cgggaatcca atttgattaa atgcgttccc tgtagttaac
     2281 cgtaaagaag cgttggacgt acacctggaa gaccctttgt ccccggcaac cgccccgccg
     2341 tcatactacc ccgggatcgt aaaatagaac gtggcgccct tgcctggttc ggattccacc
     2401 cagatgcgcc cgccgtgccg ctcaacgatt ttatcgcaga gcgcgaggcc tatgcccgtc
     2461 cccgggtatg cgctgtcctc cggatggacg cgttggaaga gctgaaatat cttctcgaaa
     2521 tgccggcgtt cgatcccgat gccgttgtcg gtcacggaga acagccagcc ccctccggcg
     2581 cagctccgcc cgtcgcccgc gtccggaacc cgccccgcct ccccggcctt ttcctccggc
     2641 gctcctgaaa gcggatgcgc cgatatcctg atctccgggg gcgcggtgct ccggtagtta
     2701 atcgcattgc cgatgaggtt ttggaacaac tggaggagct gcgtctcgtc cccggaaacg
     2761 acgggaagcc cggaggccgt gacacgggca ccgctttcgc cgaccgcaga cctgaggttg
     2821 gagacggcgg cctcgactac ggcggcgcaa tccacggggg caaggggcct cgccttcgtg
     2881 gtcacccggg catacgccag gaggtcttcg atcaggcgct tcatgcggtc cgttccctcc
     2941 atgatgtagg agagaaactt gcggccgttg atgtcgagcc ggtccccgta cttttcctcc
     3001 aatacctcgg cgaatcccgc gatggagaac agtggggcct tcaggtcgtg ggacgccgca
     3061 tgggcaaacc gctcgagttc cgcgttcgat ctcgtcagtt cccgcgtctt tttctccagt
     3121 tcctccccgg tccgcttgaa ttccgtcatg tcctgcaggg tctcgatcgc aacgataaga
     3181 cgctgtctgg cgtcgtagat gggggcggcg tcaaaaacga tgtagcggtc cttgccgttc
     3241 atatcaggat accagccctc ggcgtgaacg ccgtccggaa cgagttcgga ccgcctgaac
     3301 ttcccgtaca gggccccgag cgattcgtgt tctttgtcga tcacgaggtc cgccagagtc
     3361 ggccgctgcc ggtcgtagaa gggactccag tggttgctcg tgccgatcat ctccgcagcg
     3421 gggaacccgg tgagctcttc gcacgccttg ttccagagaa cgaccctgtg gtccgggctc
     3481 aggacgaacg ttgccaccgc ggagttgtcg actacgttct ccgcgaaccg tttctgctca
     3541 agcagcatct cttccgcgtg cctgcgtttt gtgatgtcgc ggacgtggac gacatagccg
     3601 gagaccgcct cccccccgga tgatacgagc ggcgagataa acaggtcgag atggattgct
     3661 ccccgtttgg aggtgggata gaggttgtat ttccttacct tctcgaaatc aaagacaatc
     3721 tcctgctgga ccggcctgcc ctcccggagg tcctgcttgt gccggtcgct caggttcggg
     3781 tcctcgaaga gcctgaaccc ctttaccgcc gacaggtcgt cgatgccgaa aatttccctg
     3841 caggccgcgt tcagttccac gagttcgccc ccggcgtcgt aaatttctat gccgatggga
     3901 gagctttcgt aaatagctct gaactgttga atgcttgttt ccggatcttc cgctattttt
     3961 cctccgaaca cgtcgtcccc tccctcagtg aatctggtca gaggaactta caactatcac
     4021 cggaaatttg caagggtttc gatcgggtat ctttcgggta tgcgggggga gaaggccggc
     4081 aatcgatgaa actactcgac cccttttgcc ttgagcgcct tctcgactgc ggcgatctgg
     4141 acgttgaaga gccggcattc atcctcgtac tcagccgtca ggttctgtgc cttctcggcg
     4201 ccgatatgca ccgaggtctt gccgaaggtg aaggcgtgca ggtcctccag atcgcagagg
     4261 ttgtccttca ggcgcttgag ctcggctttg agctcgtcga tggtcatgtc ttcaatgttc
     4321 tttcccattg gctcctaccc cctgtctgta tgtctggagc ggcctctggc agttgccagc
     4381 gatgcgtgac gcgtgttacg ggtttaaact cgaggtccgg aacccgcaac aaagtctttt
     4441 acatgattgt cttcagatag tcaagcgcct cttccctgct cctgatggct ccttccgcct
     4501 gcttctcctt gatctcttcc aggatctcgc ggaaccgcgg ccccggcgcg agccccagtt
     4561 ttatcaggtc gtcgcccgaa acgagcggcc gggcctcggc cttgaggaac ttcgtgtaat
     4621 agtaatcgag cacctgcgcc atgggccgtt ccgtgtccgc gaatttttcc ttcggcataa
     4681 cctcgtacgt cgcgcgcgcg tccgcctgcg acaggatcag gctttcgggc agggcgtcac
     4741 ccaggtcgcg gcaatagcgg tacatggcgt ttctgctcgg tccgcccgcg gaggaaagat
     4801 tgaagagacg catgtgatac ttgatggtct ttgcgaccaa ggcctcggta ttccgcgaca
     4861 gccggagcct cctgcagagg aagaccgcct tgtccgcgcc gaggttgtcg tggccgtgaa
     4921 agcggacgtg gccttccgat tcgctgaggg tgtcgggctt tgccatgtcg tgcagcagac
     4981 aggcgaaccg aagcgccgcg attctcggta ccccgtgttc gatctgcccg gagaggtggt
     5041 cgacgaccgc agcggcgtga gccggggaaa gggccgcgag atcgtccagt acgccgtcca
     5101 cgcagcccgc ggtcttgacg gcatgggtga ggacgtcgtg ggcccggtac ataccgggcg
     5161 tgaaggcgcg caggggctcg agctccggca gaagcggcga aaggaggccg agggagtcga
     5221 ggagcaggag gtggcttgcg gcgtcccgct gggacaggat caggaagagc tcatcccgca
     5281 cccgttcggg cgcgggtttc gcgatcgccg tcgctctctg ccggatgtgc cgtgcggtct
     5341 ccgcctcgat cgtgtagccg agcgtcgcgg cgaaacggac ggcacgaagg aggcgcaaag
     5401 ggtcctcgtc cagggccgcg ggtcgcacga cccggatcac gcggtcccgg acgtccgacc
     5461 tcccgttgaa cggatcgatc aggccttcga tggaccttcg ggcgatgaac tcccggaggt
     5521 cgagggccat ggcgttgacg gtgaagtccc tcctgccgag gtcggcaggg aggtccggcc
     5581 cttcgaagtt catgaaatca aattgaaaga aatccccgtc gtcctgcttt acgacgcggc
     5641 tgatccttct ctcttcatcg aggaaaaaaa agctcccccc gatggcgtcg gcgaaaacgc
     5701 gggccgtctc ctccgagccc gtcggcatca ggaggtctat gtccctgatc tccctgccgc
     5761 cggccaggag gtcgcggact gagccgccga cgaggtagac gggtcgaggg gtaagcgata
     5821 caagctgcga caggtatttt atgaccaggg agtccatggc ttatattagc ggtatatccg
     5881 tcagacgtca atgtccggcg ccgaagcttt gcagtcatcg tatctgatat aatcattcta
     5941 gctgatctta taaaaggtca tgatccgccg cggagacaca gagatacgga aaaaagattt
     6001 gacaaaattg aatggtttcc tgcgactccg tggttaaatt caacttctgc aatcttatcg
     6061 ttctatgttt ccgatcaagg acaccgtgcc gcgcaggacc tttcctctcg cgaccctttt
     6121 gattatcgtt gcgaacggga tcgtgttctt ttttgaactg tcgatgtcgg aacgctatca
     6181 gaacgccttc atcgagctct tcggcctggt cccggcgcgg tatacccatc ccctgtgggc
     6241 gacggtcatg ggcttccccc tggacagcta ctggccattc ctcaccaaca tgttcatcca
     6301 tggcggatgg ctgcacttca tcgcgaacat gtggacgctc ttccttttcg gcgacaatgt
     6361 ggaggaccgg ctcgggcgcc tcaggttcct cctcttttat ctcgccgccg gtatcgcggc
     6421 aaacctgatt catttttttg tgttccatga ctccacgatg ccggtcatcg gcgcttcagg
     6481 cgccattgcc ggcgttatgg ccgcctacct gcggctcttc ccccgtgcgc gcatcatcac
     6541 gctcattccg gtgcttttct ttccgttctt ctttgatgtg ccggccgtgc tgttcatggc
     6601 gatctggttc tttactcagc ttctgtccgg tgcggcatcg ctcgcgatca cggaatccgc
     6661 agggggtatc gcctggtggg cccatatcgg cggctttgcc ctgggtttcc tgttcatcaa
     6721 accgctgtgt aaaagccgct ccgatgcgtg caatcccggc gaagacttcc gctcggtctg
     6781 acggggatcc tcacgactgt gaaaaagagg gtaggaacag gctgttcaaa aggtattgtg
     6841 cgctgtcatc ctggacgacg agagggatct gaggcgcaga aaaccgggat tcttcgctgc
     6901 gttcagaatg aaattttctg gttcatccgg ctttttaaac aacctgtcaa aaaaacatcc
     6961 cgcagtcatt cttcagttga ctgcgggatg aacatgtcgg gcataacaac gccgataaaa
     7021 cgggctaatc ttccttctac gcggccctct tcatcatcac ttcgccctcg gcggcggtct
     7081 tcggggcctc gatctcgatc ttttttgcct tcgatattgc cggcaacggc gtcgtgatct
     7141 cgaggatccc gttgtggcat ttcgctttta cctgatcggc cttgatacct tcgggaagcg
     7201 taaaccgcct ctcgaagcag ccgtaggcaa atcccctgta gaagtaatcc tcgtccttga
     7261 catccttgtc cgcttttcgc gaacccttga tcacaagctg atgggtattc tcgtcaaagg
     7321 acacctcgac gtctttcgga tcgatccccg gaagctcgca cctgaacacc aggtcgttgc
     7381 ccttcttgta gctctcgatc aaaggcatcc attcgccatg gcccaggtct gcggtcgata
     7441 tttccttccc taggaactga tcgaacagcc tgcccatgtc cgcctgcaat tcgcgcagct
     7501 cgttaaaata cggggccctt tctctcatga gattgaaagg atcccatcga agcaagctct
     7561 ttgccatagt gatccctcct ctcgttgtta ccttctcgaa gtgtatctga ttagaagata
     7621 ccgcaaaacc ggggcttgtc aaatgcagca ccgatttgta gtacttccta ttcaaatact
     7681 catcgcgaac cggaggattc gtcattctaa cggcctagcg gcgtcatcgg ctctccgcct
     7741 ccggcggcgg ttccttcttc ggggcggtct cgctgttgtt ctcctccttc ttctccttct
     7801 tctcctcgcc cttgtcgtcc gacttgagct tcttcgccat ttccctgagc cgcttgtcta
     7861 ccaggaaact gaacgtgccc tcgggccagg tcccgtccgt ccggcgctcg cccgcgggga
     7921 gcccggtcag gatctcaagc gcctcgttca cggtcctgac cgcgtagacc cggaacttcc
     7981 cctctctcac ggctccgacc acttccttct tgagcatgag gttcttcaca ttgagctgcg
     8041 ggatgatcac gccctggtcg cccgtgagcc cccggttctt gcatacgctg aagaaccctt
     8101 cgatcttctc gttcacaccg ccgataggct ggatctcgcc cttctggctg atcgagccgg
     8161 tgatggcgat gttctgtctg acaggcgtct cagcaatccc ggagaggagc gcgataagct
     8221 ccgctgccga ggcgctgtcg ccttccacct ctccgtagct ctgctcaaag gcaagggtcg
     8281 cggagactga aaacgtctta tcatgcgcat aggagcccgc caggtagctc gacaggatga
     8341 gcacggcctt gttgtagatg cggcccgcga gcttcgcctc gcgctcgatg tcgatgacgc
     8401 cggacttccc cagatacacc cggctggtga tgcgcgacgg cttgccgaag gcgaagtcgc
     8461 cgatttcata aacggacagg ccgttcacct gcgcgacgac cgagccctcg acgtcgacca
     8521 tgatcgttcc ctcggtgaag agctcctgca tccgctcgtc aatgaggttc gagcgatata
     8581 ccttctcatc gagcgccttg tccacgtgtt cggccaggac cgcgccgctc cccgcccgct
     8641 gtgcccagta tgaggcctcg cgcacgaggt ccgtgaggtc gctgaaccgg agagagagct
     8701 tgtctctttt ctcggcgagc cgtgcggcgt agtcgacgac gccggccacg gcgctccggt
     8761 cgaagggcag cagtccgtcg ttcttcacca tgttcgcgat gaagttcgcg tagcgcattt
     8821 tttcgtcgga ggacgatgcg gtgcgggtgt cgaagtccga cttgaccttg aagatgtccc
     8881 ggaagtcctc gtcgtagtag tacaggaggt agtagagcca ggggcttccg agcatgatga
     8941 tcttgagcga gaccggaatg ggttcgggct tgatcccgcc cgtgccgaag ccgtagagct
     9001 catagaggtc ctcgatctt
//
LOCUS       DASSLD010000375         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1574329, whole genome shotgun sequence.
ACCESSION   DASSLD010000375 DASSLD010000000
VERSION     DASSLD010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574329"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFK23_06775"
     CDS             complement(<1..323)
                     /locus_tag="VFK23_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HET7318820.1"
                     /translation="MIISKADDENGHDSPRQASAPGRSRIALLVGFCLFAVLASAQPL
                     FAALGEPADSVARDRRAMSAALGKTTEHTGYTVQEVVSDSTTVREYLTPSGIVFGIAW
                     NGLTHP"
     gene            complement(530..1189)
                     /gene="epsC"
                     /locus_tag="VFK23_06780"
     CDS             complement(530..1189)
                     /gene="epsC"
                     /locus_tag="VFK23_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HET7318821.1"
                     /translation="MIETCKKIRRDFHAALSMDPAATSRVEVALTYAGFHALLFYRFA
                     HWLWKYRIPFIPRALSQFARCMTGIEIHPGASIGSGLFIDHGMGVVIGETTEIGDNVT
                     LFQGVTLGGTGKQRGKRHPTLGSHVVVGAGAKVLGPITIGDYVKIGANSVVLQDVPDH
                     STVVGIPGRIVRIKDERVPDEALLDHIHIPDPIADRLIDLQLQIDMLKKELDKRGKKE
                     E"
     gene            complement(1352..1912)
                     /locus_tag="VFK23_06785"
     CDS             complement(1352..1912)
                     /locus_tag="VFK23_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HET7318822.1"
                     /translation="MRSGLLIAVFLIMSALPAPAQTAGNADEKQSYLYEWTDGKGVVH
                     ITDSLGKVPEQYRTNARRLETSPEEGATPNRPQQGTISPSGNTEDQREAQQKAQWQRR
                     MIDAKQRLAAAEQRYRELEQRRATLLGQWGTPAYAPPAARIEAEKLEGDMQNVQKEID
                     NARNEVEVVIPEEARKAGVPPGWLRE"
     gene            complement(1916..>2235)
                     /gene="ispF"
                     /locus_tag="VFK23_06790"
     CDS             complement(1916..>2235)
                     /gene="ispF"
                     /locus_tag="VFK23_06790"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET7318823.1"
                     /translation="LGDIGRHFPDSDPKYKGISSLVLLEEVGRLLAEGGFRVHNIDAT
                     IVAERPRMAPHIPAMAANIAAAAGVERSSVNVKATTTEGLGYTGRGEGMAAYAVCLIR
                     KAE"
BASE COUNT          405 a          676 c          633 g          521 t
ORIGIN      
        1 gggtgcgtca ggccgttcca ggcgatgccg aatacgatac cggacggtgt aagatattca
       61 cgaaccgtcg ttgaatcgga tacgacctcc tgaacagtat aacccgtgtg ttccgttgtc
      121 ttaccgagcg ctgccgacat cgccctgcgg tcccgggcga cagaatcggc aggttctccg
      181 agcgcggcaa agagcggctg ggccgatgca agcaccgcga aaaggcagaa accgacaagc
      241 agggcgatac ggcttctccc cggagccgaa gcttgcctcg ggctgtcgtg tccgttttca
      301 tcatccgcct ttgaaatgat cattttcttc tccctctggt ttcgattcgt aatgtctgga
      361 tttccggcat gaaccaacta cggaaaaatt gagataatct gtggaatgcg cttcgtgcat
      421 gtccgggctt aagcacatat tactatttta gaacaaattc gtaaataatc aattgtctcg
      481 tctcgaaaaa aatcaggaac tgccgggaag gaggtttcag gcgcggtttc tattcttctt
      541 ttttcccccg tttgtccaat tccttcttga gcatgtcgat ctggagctga aggtcgatga
      601 ggcggtccgc gatggggtcc ggaatatgaa tgtgatcgag gagcgcttca tcgggcacgc
      661 gctcgtcctt gatccgcacg atcctgccgg gaatgccgac cacggtggag tggtccggca
      721 cgtcctggag cactaccgag ttcgcgccga tcttcacgta gtcgccgatg gttatggggc
      781 ccaggacctt ggcgcccgcg cccaccacga catggctccc cagggtagga tggcgcttcc
      841 cccgctgttt gccggtcccc cccagggtga cgccctgaaa gagcgtgacg ttgtccccga
      901 tttcggtggt ctcgccgatc accacaccca tcccgtgatc gatgaacagc cccgatccga
      961 tcgaggcccc tggatgaatc tcgatcccgg tcatgcatcg tgcgaactgc gacagggcgc
     1021 gggggatgaa gggaatgcgg tatttccaga gccagtgcgc gaagcggtag aacaggagcg
     1081 cgtgaaaccc cgcataggta agggccacct ccaccctgct cgttgcggcg ggatccatgg
     1141 agagggccgc gtggaaatcc ctccggattt ttttgcaggt ctcgatcatg gtgttcgaat
     1201 gtggaatgcg gaattcggaa tgcggaagga aaaacttgtt acggggagaa tgcagtgaat
     1261 accgcggtga ggaaaccgtc ggacgaataa ctctccttac gttcccagcc tctggtttaa
     1321 ttccgcattc cgcattccgc attcatcttt attattccct cagccacccc gggggcactc
     1381 cggcctttct cgcctcttcc gggatcacga cctccacctc gttccgtgcg ttgtcgattt
     1441 ccttctggac attctgcatg tctccctcga gcttttccgc ttcaatcctt gcagctggcg
     1501 gcgcataagc cggggttccc cattgaccaa gcagcgttgc tcgtctctgt tcgagttccc
     1561 ggtaccgctg ttctgcggcg gcgagacgtt gtttcgcatc gatcatgcgt cgctgccact
     1621 gggccttctg ctgcgcctcg cgttggtctt ccgtgtttcc cgacggcgag atcgtgccct
     1681 gctgcggccg gttcggcgtc gccccttctt ccggggaggt ttcgagtcgc cgcgcattgg
     1741 tccggtactg ttccgggact ttcccgaggc tgtcggttat gtgaacgact cctttgccgt
     1801 ccgtccactc gtagaggtat gattgctttt cgtccgcgtt gcccgccgtc tgcgccggcg
     1861 ccggaagcgc cgacatgatg aggaacaccg caataagaag tcccgatcgc atgagctact
     1921 ccgctttcct gatcaagcag acggcatacg ccgccatgcc ctcgcccctc ccagtataac
     1981 cgagcccttc ggtggtcgta gccttcacgt tgaccgacga tcgctccact ccggccgctg
     2041 cggcgatatt cgccgccatg gccgggatgt ggggcgccat tctcggccgc tccgcgacaa
     2101 tggttgcgtc gatattgtgt acgcggaacc ctccttcggc gaggagccgc cccacttctt
     2161 cgagcaaaac aaggctggat atgcctttat acttcgggtc ggaatccggg aaatgcctgc
     2221 cgatgtcccc aagcc
//
LOCUS       DASSLD010000376         2763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1141564, whole genome shotgun sequence.
ACCESSION   DASSLD010000376 DASSLD010000000
VERSION     DASSLD010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141564"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /gene="murJ"
                     /locus_tag="VFK23_06795"
     CDS             <1..991
                     /gene="murJ"
                     /locus_tag="VFK23_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HET7318824.1"
                     /translation="QLPGLRKQGMMLELKFTPTHPGAGKIARLAAPVFFSSSVNQFNI
                     FIGTIFASFLATGSISYLFYGMRFIHFPLGIFGIAIATAILPTMSAQAAKRETEAFRE
                     TLSFGLRLVFFVMFPAMAGLITLRVPIVNLLLEHGKFDRMSTQGVAAALLYYAVGLWA
                     MAGVRIVAQAFYALQDTKTPVKIAVLALFANIAFSAAFIAWTPLNHGGLALATSLAAM
                     LNISLMTVQLRKKIGRMDGRRIFASLLKIVPASLAMGVIGWLVSRQPVWELGGHSVLK
                     AELLAGGMTASVLFYIIVMRLLKSEELAFVWEMVRRRKNRWGEGATGRGGEGD"
     gene            993..1391
                     /locus_tag="VFK23_06800"
     CDS             993..1391
                     /locus_tag="VFK23_06800"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET7318825.1"
                     /translation="MGKAIFHTSLGWVGVEVSEQGILRVVLPKKTKGAVVRELHGEAT
                     RSTAVSPVLNKAVDLLGRYFAGERVLFDLPLDLRYYTPFQRAVWRAAEEIPCGETRSY
                     SWVAMRIKNPRAARAVGGALGANPVPVLVP"
     gene            1579..2217
                     /locus_tag="VFK23_06805"
     CDS             1579..2217
                     /locus_tag="VFK23_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7318826.1"
                     /translation="MILIRLIVGPLQVNCFILADEKTKEAVVIDPGDDAQDILKVIQE
                     KGLRVRYIVNTHAHFDHVGANKAVKDATGAELLLHEADAPLLAAAASQSRSFGMGPVS
                     SPPPDRLLKHGDRIAAGEVSLTVVHTPGHTPGGISLIEEGMVFTGDSLFAGSIGRTDF
                     PGGDLMALIGSIKRHLLSLPDDTKVFSGHGPASTIGDERRENPFLNASSGFE"
     gene            2362..>2763
                     /locus_tag="VFK23_06810"
     CDS             2362..>2763
                     /locus_tag="VFK23_06810"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET7318827.1"
                     /translation="MVVGPIREGLTFDDVLLLPAKSDVLPRDANVRTHLTKRVTLNIP
                     LVSAAMDTVTEAKLAIALAQEGGLGFIHKALPIGQQAEEVDKVKKSESGMIIDPITIA
                     PDAKISDALELMKKYRISGVPVTKGQKLVGIL"
BASE COUNT          574 a          777 c          836 g          576 t
ORIGIN      
        1 tcaattgccg gggctcagga aacaaggtat gatgcttgaa ttgaaattta ctccgaccca
       61 tcccggcgcg gggaagatag cgcgccttgc ggcgcccgtc tttttcagct cctcggtaaa
      121 ccagttcaat atcttcatcg gcactatttt cgcatcgttt ctcgccacgg gcagcatcag
      181 ctacctgttc tacggcatgc gcttcatcca ttttccgctc ggcatcttcg gcatcgccat
      241 cgcgacggcc atactcccaa ccatgtcggc gcaagcggca aaacgcgaaa cggaggcatt
      301 ccgcgagacg ctgtcgttcg gactccggct cgtgtttttt gtcatgttcc cggccatggc
      361 cggactgatc accctgcgcg tcccgattgt gaaccttctg ctggaacacg ggaagttcga
      421 ccgcatgtca acgcagggcg tggccgcagc gctcctgtac tatgccgtag ggctttgggc
      481 catggcaggg gtacggatcg tggcccaggc tttctatgcg ctccaggaca ccaaaacacc
      541 ggtcaagatc gccgtattgg cgctgttcgc gaacattgcg ttcagcgccg ccttcattgc
      601 atggacgccg ctcaatcacg gcggtcttgc ccttgcgacc tcccttgccg ccatgctgaa
      661 catcagcctc atgaccgttc agctccggaa gaagatcggc cggatggacg ggaggaggat
      721 cttcgcctcc ctcctcaaga tcgtgcccgc gtcccttgcc atgggtgtga tcggctggtt
      781 ggtcagccgg cagccggtct gggaactcgg cggacactcg gtcctgaagg cagagctgct
      841 tgcgggcggg atgaccgcga gcgttctttt ttatatcatt gtgatgcggc tattgaagag
      901 cgaagagctg gcattcgtgt gggagatggt gaggcggagg aagaaccgat ggggggaggg
      961 ggcgacgggg agagggggag agggggatta gcatggggaa ggcgattttt cacacctcgc
     1021 tcggctgggt cggcgtggaa gtatcggagc aggggatcct ccgcgtcgtt cttccgaaaa
     1081 aaacgaaagg ggccgtcgta agggaactgc acggcgaggc aacgcgttcc accgctgttt
     1141 ctcccgtgct caataaggcg gtagatctgc tcgggagata ttttgccgga gagcgcgttt
     1201 tatttgacct gccgctcgat ctgcgttatt atacgccctt tcagcgggcg gtctggcggg
     1261 cagcggagga gatcccctgc ggggagaccc ggtcctattc ctgggtggcg atgaggatca
     1321 aaaatccgcg ggccgcgcgt gcagtgggcg gagcgctcgg ggccaatccc gtcccggtcc
     1381 tggtcccctg acaccgcgtg atcggctcgg caggttcgct gggcgggttt accggcggga
     1441 tcgggatgaa gaagaagctg ctggatcttg aaaacaacac gtttcttaac cgtggaggac
     1501 gcagagaaaa agagtcaaaa gcaaaagatt caaaaacttt gcgttctcgg cggtctctgc
     1561 ggtgagaaag acaatatcat gatcttaatt cgtctcatag tcggccccct tcaggtgaac
     1621 tgcttcatcc tggccgacga gaagacaaaa gaggcggtcg tcatcgatcc cggagatgac
     1681 gcgcaggaca tcctcaaggt cattcaagag aagggactca gggtcagata catcgtgaac
     1741 acccatgctc atttcgatca cgtgggtgcg aacaaggcgg tcaaggacgc cacgggcgcc
     1801 gaactgctgc tccatgaggc ggacgccccc ctccttgccg ccgcggcgag tcagtcacgg
     1861 tctttcggca tgggccctgt ttcgtctccg cctccggacc ggctgctgaa acacggcgac
     1921 aggattgctg caggcgaagt gtcgctcacg gttgtgcata cgcccggcca cacgccggga
     1981 ggcatcagcc tgattgagga aggcatggta tttaccggcg attccctgtt tgccggatcg
     2041 atcggcagga cggacttccc gggcggcgat ctcatggccc tcatcggatc gatcaagagg
     2101 caccttctga gcctgcccga cgacaccaag gttttttccg gccatggacc agcctcgacg
     2161 atcggcgacg aaaggcggga aaatcccttt ttaaacgcgt catcggggtt tgaatgacac
     2221 ggttttcctt gaggttttca gcccggtgtg gtagagtaac agaattccga tacacgatac
     2281 cggatgcatg atgcggacag aacagctttt atcatggatc gtgcatcatg gatcatgcat
     2341 cattgaataa aggagaactc gatggttgtc ggtcctatcc gagaaggtct tacattcgac
     2401 gacgtgctgc tgttaccggc taaatccgat gtacttccca gagacgccaa tgtccggacg
     2461 catctcacca agagggtgac ccttaacatc cccctcgtta gcgctgccat ggacacggtc
     2521 accgaggcaa agcttgccat cgcccttgcc caggaaggcg gactggggtt catccacaag
     2581 gcgctcccca tcggtcagca ggccgaggag gtggacaagg tcaagaagtc ggaaagcggc
     2641 atgattatcg accccatcac cattgcgccc gacgccaaga tttccgacgc cctggaactc
     2701 atgaagaaat accgcatatc gggcgttccg gtaacgaaag gccaaaagct ggtcggcatc
     2761 ctg
//
LOCUS       DASSLD010000377         6074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_1002,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000377 DASSLD010000000
VERSION     DASSLD010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFK23_06815"
     CDS             complement(<1..513)
                     /locus_tag="VFK23_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HET7318828.1"
                     /translation="MTPRNFVCPLPVPESAPLRIAVAGNPNSGKSTLINSLAGSRLHV
                     GNWPGVTVEKREASFERRGERIALIDLPGTYSLSPYTEEEVVARDYLVQERPDVIIDV
                     VDVTNMERNLYMTVQLLELEIPVVVALNIFDEAEKKGYRIDVPAMEQALGVRIVPTVA
                     TKGTGLDALLE"
     gene            complement(510..737)
                     /locus_tag="VFK23_06820"
     CDS             complement(510..737)
                     /locus_tag="VFK23_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HET7318829.1"
                     /translation="MNLAMLKPGQSGRISHIGAIGSLKRRLMDMGVLVGEEVKVEKIA
                     PLGDPIGITVKNYNLSLRKREAEGILLEVPK"
     gene            complement(734..1015)
                     /locus_tag="VFK23_06825"
     CDS             complement(734..1015)
                     /locus_tag="VFK23_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HET7318830.1"
                     /translation="MFPLGLLANGERAEVVNGIPAFEHGQGPCCGQCRGKGREGSAVR
                     LEDMGIRPGKEVEMLNNRGGGSLLVKVDESRIAVGRGMAMKIMVRRQGR"
     gene            complement(1091..2299)
                     /locus_tag="VFK23_06830"
     CDS             complement(1091..2299)
                     /locus_tag="VFK23_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HET7318831.1"
                     /translation="MKKPSDPLQFGPVKLGTDALMRAETATNFRLGSFSFTPGSNEGR
                     ILFRLRPSLTVSPSENLNARVEGQWYAFYDDRDVSEFTLYQGFVEGTVPGAKKLSVKV
                     GRQEFVYGSGFLLGNDAFYDGLSIDALKATLKPANGVSVDLFGGQYARRNSGGIAGKL
                     YGIYATYAPRETLSVDLYGFRDTGGVGETHAGGDHERTYSVGTRISGNIAGKKTAFEV
                     EPVYQFGRKGREDGVSHNDISACGGHVDVTVDPPLGRYPGKIFLSYAYGSGDGNPKEG
                     TFTEFHNPNNDTSLIGDMNVIGELGGLTVGNVTASGLHVVTVGAGIAVTEKLNVSLDG
                     HHFRAVKTPAGISKEIGIETNLIFTCKIDDHISVLLSGNRFFTGGFFKDASGSGKDIS
                     YAYLQAQATF"
     gene            complement(2644..3003)
                     /locus_tag="VFK23_06835"
     CDS             complement(2644..3003)
                     /locus_tag="VFK23_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318832.1"
                     /translation="MEARENNSSRQDSWSPAREEATAKEVRGDMRVEEYVDRIAEEMH
                     ERVEFQRRLFKQVLAGGGNALDCPAYCPLVDCPRLSKLQGALRETIEIFEETRSSFKS
                     RKLEVMRKRLIDILAGS"
     gene            complement(3066..3443)
                     /locus_tag="VFK23_06840"
     CDS             complement(3066..3443)
                     /locus_tag="VFK23_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7318833.1"
                     /translation="MGSPAVKRDTATAEKLAYIDAASVRAARTAMPGDRMVRALAETF
                     SALSDPTRIRIISALGEQELCVIDLARFLGLTGSAVSHQLRLLRGQRLVKYRKEGRIA
                     YYSLDDDHIRNLMEECIKHVSVG"
     gene            complement(3681..4112)
                     /locus_tag="VFK23_06845"
     CDS             complement(3681..4112)
                     /locus_tag="VFK23_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET7318834.1"
                     /translation="MKTTPGDGELLTKKSLSLGDAKKIAAGAAAEARKNKWNLDIAVV
                     DDGGHLIYFERTDGTLLASEDLAIGKARTAVAFQRPTKALEDALNSGQYAILSYPNAI
                     PREGGLLIVVDGKIVGGIGVSGCKPSEDAQVAKAGVDAFSK"
     gene            complement(4139..5023)
                     /locus_tag="VFK23_06850"
     CDS             complement(4139..5023)
                     /locus_tag="VFK23_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7318835.1"
                     /translation="MALQKLGKTADAGGMGYLTSLPRKAVTIYMPIVVFLIALLFPFY
                     WMTITAFKPNEELLSHVGNPFWVHAPTLAHFKKLLFDTAYPEWLWNTVLVSVVATFIS
                     LVCSVLAAYAIERLRFKGSRGIGMAIFLAYLVPPSILFIPLAAMIYKLHLFDTRLALI
                     LTYPTFLIPFCTWLLMGYFKSIPFELEECALIDGASRLQILTKIILPLAVPGMISAGI
                     FAFTLSWNEFIYALTFVSSSEVKTVPVGVITELIEGDVYHWGALMAGALLGSLPVAVL
                     YSFFVEYYVSGMTGAVKE"
     gene            complement(5035..5925)
                     /locus_tag="VFK23_06855"
     CDS             complement(5035..5925)
                     /locus_tag="VFK23_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7318836.1"
                     /translation="MTLSRLVENRNFLGAVFMLPAVLLLLVFLTYPLGLGTWLGFTDA
                     KIGRDGLWVGLGNYIYLFGDQVARLSLFNTLIYTAVATAVKFVLGLWLALLLNKNLPF
                     KSFVRAIVLLPYIVPTALSALAFWWIYDTQFSILSWGLMRLELIDHYIDFLGEPWLAR
                     ASVIVANVWRGVPFIAICLLAGLQTISPSYYEAAVLDGGTPWQMFRYITLPLLTPIIA
                     VVMTFSILFTFTDFQLIYVLTRGGPLNATHLMATLSFQRAIPGGSLGEGAALATAMVP
                     FLLAAILFSYFGLQRRAWQQ"
BASE COUNT         1212 a         1880 c         1771 g         1211 t
ORIGIN      
        1 ctcaaggagc gcgtcgagcc ccgttccttt tgtggcgacg gtcggaacga tccggacgcc
       61 caaggcctgc tccatggccg gcacgtcgat acggtacccc ttcttctccg cctcgtcgaa
      121 gatgttcaag gcgacgacga ctgggatctc cagctcgagg agctggacgg tcatgtacag
      181 gttgcgctcc atgttggtga cgtcgaccac gtcgatgatg acatcaggcc gctcctggac
      241 caggtagtcc ctggcgacga cctcctcttc ggtgtacggg cttaagctgt acgtgccagg
      301 cagatcgatc agcgcgatcc gttcaccgcg ccgctcgaag gacgcctctc tcttctccac
      361 tgtgacgccg ggccagttgc ccacatgcag ccggctgccc gcaagggagt tgatcagggt
      421 agatttcccc gagttgggat tccctgccac cgcgatgcga aggggtgcgg attccgggac
      481 aggtaacgga cagacgaaat tccgtggcgt catttcggaa cctccagcag aattccttcc
      541 gcctctctct tcctcaggga gaggttgtag ttcttcaccg tgatcccgat ggggtcgccc
      601 agcggagcga tcttttccac ttttacctcc tcgccgacca gcactcccat gtccatcagc
      661 ctcctcttca aggacccgat cgcgccgata tgcgagatcc gcccgctttg tcccggtttg
      721 agcatcgcca ggttcatcgt ccctgcctcc tcaccatgat tttcatcgcc atgccgcgcc
      781 ccaccgcgat ccgggactcg tccaccttta cgaggaggga cccgcctccc cggttgttga
      841 gcatttcgac ttccttgccg ggccggatgc ccatgtcttc cagccggacg gcggagcctt
      901 cccgtccctt gcccctgcat tggccgcaac aaggcccttg gccgtgctcg aaggccggaa
      961 tcccgtttac cacctccgcc ctctcgccgt ttgccagcaa tcccagcgga aacataccgt
     1021 tacctcccta catgcttggg aattttgaat gcttcgtcat gcatcccgcc tgtgtcccga
     1081 atccggttct tcaaaacgta gcctgcgcct ggagataggc gtacgagatg tccttcccgc
     1141 tcccggaggc gtccttgaag aaaccgccgg tgaagaaacg gttcccgctc aacaaaaccg
     1201 agatgtggtc atcgatcttg caggtgaaga tcaggttcgt ttcgatgccg atctccttgc
     1261 tgatcccggc aggcgtctta acggccctga agtggtggcc gtccagggag acgttcagct
     1321 tctccgtcac ggcgatcccg gcgccgaccg tgacgacatg gagaccgctg gccgtcacgt
     1381 tgccgaccgt cagacccccc agttcgccga tgacgttcat gtccccgatg agggaagtgt
     1441 cgttgttggg gttgtgaaac tccgtgaacg tcccctcctt cgggttcccg tcgccggagc
     1501 cgtaggcgta tgaaaggaag atcttccccg gataccgccc caggggagga tcgacagtca
     1561 cgtctacatg gccgccgcag gcgctgatgt cgttgtggct gacgccgtcc tcgcgcccct
     1621 tcctcccgaa ctggtagacc ggctccactt cgaaggccgt ctttttcccg gcgatgtttc
     1681 ccgagatcct ggttcccacc gaataggtcc tctcgtgatc tccgcccgca tgcgtttccc
     1741 cgacaccgcc ggtgtcgcgg aatccgtaga gatcgaccga aagggtctct ctcggggcat
     1801 aagtcgcata gatcccatag agcttcccgg cgatcccgcc cgaattcctc cttgcgtatt
     1861 gacccccgaa caggtcgaca gagactccgt tggcaggttt aagcgttgcc ttcaatgcat
     1921 cgatagacag gccgtcgtaa aaggcgtcgt tgccaagcag gaagccgctc ccatatacga
     1981 actcctggcg gcccactttc acggagagct ttttcgcgcc ggggacagtc ccttccacga
     2041 acccctggta cagggtgaac tcggaaacgt cccgatcgtc gtagaaggcg taccattgtc
     2101 cctccactcg ggcgttcagg ttctccgaag ggctgacggt caacgacggt cgaagccgga
     2161 acagaatccg gccctcattg ctccccggcg taaaggagaa gcttccgagg cggaaattcg
     2221 tcgcggtctc ggcccgcatc agggcgtcgg tgccgagctt caccgggccg aactgcaagg
     2281 ggtccgaagg ttttttcacg ccatccgcca tttggacggt ttcagaacta catggcggat
     2341 gatcctgggc ccgggccggt cccgcccgcc acaggatcaa cagcgcgagg accgccacgg
     2401 cggggagaag cccacacttt ccgtaggaaa gagatggaag gacaaacgag taagaacgat
     2461 tcggtttcgg tacgggattc aagccgcaca ctcctctcat tcgtaaagaa gtattgaagg
     2521 caacaacctg tatggcccgg gcgcgtcgta tgcacgccct caagttgcgc tttcactgtg
     2581 cggttctggg agaggaaatg ctgctttgtc gcccgatccg taccgtaaaa cgcaccttgc
     2641 cggtcagctc ccggcgagga tgtcgatcag cctctttcgc atcacctcca atttcctgga
     2701 tttgaaggaa cttcgcgttt cctcgaaaat ctcgatcgtt tcccgaaggg cgccctgcag
     2761 tttggaaagc ctggggcagt cgaccagggg gcaatatgcc ggacagtcca aggcgttccc
     2821 tccacctgca aggacctgct tgaagagacg gcgctggaac tccacccgct cgtgcatctc
     2881 ctcggcgata cggtcgacgt attcctcgac cctcatatcg ccgcggactt cctttgcggt
     2941 cgcctcctcc cgtgcggggg accacgaatc ctgccggctc gaattatttt ccctggcctc
     3001 caaatgcttc cccctgccaa tggcgtacat atgagcagat gtttcattca taagataaaa
     3061 aaatattacc ctacggagac gtgcttgatg cattcctcca tgaggttccg gatgtgatcg
     3121 tcgtccagcg aataataggc gatccttccc tccttgcggt atttcaccag gcgctggccc
     3181 cgaaggagcc ttagttggtg cgaaaccgcc gatccggtaa gcccgaggaa ccgggccaga
     3241 tcgatgacgc agagctcctg ctcccccagc gcggagatga tccggattcg cgtagggtcg
     3301 cttaaggcgc tgaacgtttc cgcaagagcc cttaccatcc ggtctcccgg catagcggtt
     3361 cttgccgcac gcacgctcgc agcgtcgatg taagcaagtt tctccgccgt tgccgtgtcg
     3421 cgtttcactg ccggtgaacc caacgatgat gcccctttca ttgaaacgat tgctcactta
     3481 tagcaaattg aagcgaaggc tgtcaataca aaaaacctgg cccccctgaa tggctccaat
     3541 ggggcgaagc attcgctctg taacggggaa acggccggcg atgtcgggca cggttgaccc
     3601 ggtttggccc tttgaagcta ccgaaatcat tgaagcggta gccccttttt cgttctgttc
     3661 gcatcccgtt cgcattccat ctacttcgag aacgcgtcga ctcccgcctt ggcaacttgg
     3721 gcgtcctccg agggcttgca accgcttaca ccgatcccac ccacgatctt cccgtccacg
     3781 acaatcagca gaccaccctc gcggggtatg gcgttcgggt aggacaggat cgcatattgc
     3841 ccactgttaa gggcatcctc gagagctttt gtcggccgtt ggaatgccac cgcggttcga
     3901 gccttcccga tcgcgagatc ctcgcttgcg agcagtgtcc cgtccgtccg ctcgaagtag
     3961 atcagatggc ctccatcatc gacgacagca atgtccaggt tccatttgtt cttccgggct
     4021 tcagcagccg ctccggcggc aatcttcttg gcatccccca gcgacagact ctttttcgtc
     4081 aggagttccc cgtctccggg tgtcgtcttc atgttcccct cccgtgttga atagctatct
     4141 actccttcac cgccccggtc atgccggaaa cgtagtattc gacgaagaag gaataaagca
     4201 cggccaccgg cagcgacccg agcaatgcgc cggccatcag cgccccccag tggtagacgt
     4261 cgccctcgat gagttccgtg atgacgccaa cgggtacggt tttaacctct gaagaggaaa
     4321 cgaatgtaag ggcatagatg aactcgttcc acgacagcgt gaaagcgaaa attccggccg
     4381 agatcatgcc aggcaccgcc aacggcagaa tgatcttcgt gaggatttgc aggcggctgg
     4441 caccgtcgat cagtgcgcat tcctccagtt cgaagggaat cgacttgaag taacccatca
     4501 agagccaggt gcaaaaaggg atcaggaagg tcgggtaggt gaggattagc gccagccggg
     4561 tatcgaacag atgcagcttg tagatcatcg ccgccaaagg gatgaagagg atggacggcg
     4621 gtaccaggta agcgaggaag atcgccatgc cgatgccgcg cgagcccttg aaacgcaggc
     4681 gctcgatggc gtaggcggcg agcacgctgc acacgagcga aatgaaggtc gccaccaccg
     4741 agaccagcac ggtattccac agccattccg gatatgcggt gtcgaacagc agcttcttga
     4801 agtgggccag ggtcggcgca tgcacccaga aggggttgcc cacgtgggag agcagttcct
     4861 cgttgggttt gaaggccgta atggtcatcc agtagaaggg gaacagcagc gcgatcagga
     4921 acaccacaat cggcatatag atcgtcaccg ccttgcgcgg cagtgaggtc aggtagccca
     4981 tgccgccggc atcggccgtc tttcccagtt tctgaagtgc catgtcggtt ttccttattg
     5041 ctgccaggcc cggcgctgga ggccgaagta gctgaacagg attgccgcca gcaggaaagg
     5101 caccatggct gtggccaatg ctgcgccctc gccgagagag ccgccgggaa tcgcgcgctg
     5161 gaacgacagc gtcgccatca gatgcgtggc gttgagcgga cccccgcgcg tcagcacgta
     5221 gatcaactgg aagtcggtga aggtgaacag gatcgagaag gtcatcacca ccgcgatgat
     5281 cggtgtcagg agcggcagcg tgatgtagcg gaacatctgc catggcgtgc cgccgtcgag
     5341 caccgccgcc tcgtagtagg acggagaaat ggtttgcagg cccgccagca ggcagatggc
     5401 gatgaatgga acgccgcgcc agacgttggc gacgatcacc gaggcgcgcg ccagccaagg
     5461 ctcgccgagg aagtcgatgt agtgatcgat cagctcaagg cgcatgaggc cccagctgag
     5521 gatggagaac tgcgtgtcgt agatccacca gaaagccagc gccgaaagcg ccgtgggcac
     5581 gatgtagggc agcagcacga tggcgcggac gaaggacttg aacgggaggt ttttgttcag
     5641 cagcaacgcc agccagagtc cgaggacgaa cttgacggct gtcgcgacag ccgtatagat
     5701 cagggtgttg aaaagcgaga gtcgcgccac ttgatcgcca aacaggtaga tgtagtttcc
     5761 gaggccgacc cagagcccgt cgcgaccgat cttggcatcg gtgaagccga gccacgtgcc
     5821 cagcccaagc ggataggtaa ggaagaccag cagcagcaga accgcgggca gcatgaagac
     5881 ggcgccgaga aagttgcggt tttccaccag gcgcgaaagg gtcatgggca tatgctccgg
     5941 gaacaggggt cacccggggc ggcgtgggcc gccccgggtt cgccggcggt cagatcttgt
     6001 agtagcgctc ggcgcgcttc tgcgcgcgct ccgccgccgc cttgggcgtt tgctggcccg
     6061 aggcggcctc cgcc
//
LOCUS       DASSLD010000378         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_841257,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000378 DASSLD010000000
VERSION     DASSLD010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841257"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VFK23_06860"
     CDS             <1..813
                     /locus_tag="VFK23_06860"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7318837.1"
                     /translation="GNNAGIAVARGRYIALLNNDTKVDPLWLESLIDAAEASPHRVGM
                     WACKILSYDKPDMIDNVGLLLYLDGLGRGHGRLEEDRGQFDLPGEAFAPSGCAGLYRK
                     EMLDEIGLFDEEFFAYADDVDIGLRARLAGWECGYVPSAKVYHKYSASSSAYSSFKAF
                     LVERNRIWVLLKYFPMELVLASPFFTFLRLLLHLAGAISGQGASGKFSEQHSVFRALL
                     ILFKAWGAALAGVPGICRQRRAFYPLKRLSRPGLYRLLCRYRISAREVALKE"
     gene            1007..1702
                     /locus_tag="VFK23_06865"
     CDS             1007..1702
                     /locus_tag="VFK23_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7318838.1"
                     /translation="MKTLIIIPAYNEEASIPGVIRDLREYVPMVDVLVVNDGSRDGTS
                     RLARSMGVKVLDLPFNLGIGGAVQAGYLYALQNGHDIAVQFDGDGQHCACEVKKLLDP
                     VEAGRVDFVVGSRFLASGEYRTPIFRRLGIGIFSIVLSRILGIKITDSTSGFRAGNRR
                     VIEFFARTYPDDYPEVESLVLLHKMNMRIEEVPVAMRERTGGKSSITPVRSAYYMTKV
                     LMAIFIDLMKKVR"
     gene            1705..2079
                     /locus_tag="VFK23_06870"
     CDS             1705..2079
                     /locus_tag="VFK23_06870"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HET7318839.1"
                     /translation="MDLLKILAVFGSVALLVVVVELIRRGRLKERYSLLWLLAGGLLL
                     FLSLSRRVLESLAGLFGIFYPPSLLFLLAFIFLLLITLHYSVVLSGLSEKNKQLAQEV
                     ALLRREIEEALKGDKEKRETRG"
BASE COUNT          466 a          543 c          679 g          520 t
ORIGIN      
        1 ggcaataatg ccggcattgc ggtcgcgagg ggcaggtata ttgccctcct gaacaacgat
       61 acgaaggtcg atcctttatg gcttgagagc ctgatcgacg ccgcggaggc gagtccgcac
      121 cgtgtcggga tgtgggcctg caagatcctc tcctacgata aaccggacat gatcgataat
      181 gtcggtctgc tcctgtacct cgacgggttg ggcagggggc acggaaggtt ggaagaggac
      241 aggggacagt ttgacctccc gggagaggca tttgccccca gcgggtgcgc cgggctctat
      301 cggaaagaga tgctggatga aatcggattg ttcgacgagg agttcttcgc ctatgccgac
      361 gacgtggaca tcgggttgcg cgcaaggctt gcgggatggg agtgcgggta cgtcccgtcc
      421 gcgaaggtct atcacaaata ttcggcttct tcttccgcct attcgtcgtt caaggccttc
      481 ctggtcgaac gcaacaggat atgggtcctg ttaaagtatt tcccgatgga actcgttctg
      541 gcgagcccct tctttacgtt cctgcgcctg ctgcttcatc ttgcaggggc catctcgggg
      601 cagggcgcaa gcgggaagtt ttccgagcag cactccgttt tcagggcctt gctgatcctg
      661 ttcaaggcgt ggggtgcggc ccttgcggga gttcccggca tctgccgcca gcgaagggca
      721 ttttaccctt tgaagcgtct cagccggccc ggattatacc gcttgctgtg ccggtatcgg
      781 atatcggccc gcgaggtcgc actgaaggaa tgaaggtgat gctcgcggat gaaagattaa
      841 ccgcggagac gccgagacgc cgagtgaaaa acagtaaagg agataatata tttttgcaat
      901 tgagatccat aaaagattaa cccggctttc ttgagaattt attggggagg ttgtcagttt
      961 tctgcgtctt cgcgcctctg cggtaaaatc gggattacgt gaactcatga aaacactgat
     1021 catcataccg gcatacaatg aagaagccag catcccggga gtgatccggg acctgagaga
     1081 atatgttccc atggtcgacg tgctcgtcgt caacgacggg tcgcgggacg ggacgtcgcg
     1141 tttagcccgg tcaatgggag tgaaggtgct ggatctgccg ttcaacctcg gcatcggcgg
     1201 cgcggtccag gcggggtacc tgtacgcgct gcagaacggc catgatatcg cggtgcagtt
     1261 cgacggagac gggcagcatt gtgcctgtga ggtaaagaaa ttgctcgatc cggtggaggc
     1321 ggggagggtg gacttcgtgg tcgggtcgcg tttcctggcg tccggcgaat acaggacgcc
     1381 gatcttcaga aggctcggca tcgggatatt ctcgattgta ctttcccgca tcctgggcat
     1441 aaagatcacc gacagcacgt ccggctttcg ggcagggaac cgcagggtga tcgaattctt
     1501 cgcccgaacc tatcccgacg attatcccga agtggagtcg ctcgtccttc tgcacaagat
     1561 gaacatgcgg attgaggaag ttcctgttgc gatgcgagag aggaccggcg gcaaatcctc
     1621 catcacgccc gtccggtcgg cctattacat gacgaaggtg ctcatggcca tctttatcga
     1681 tctgatgaag aaagtgaggt aaccatggac ttgttgaaaa tactggccgt gttcgggagc
     1741 gttgccctcc tggtcgtggt ggtggaactc atcaggaggg ggagattgaa ggagcggtat
     1801 tcactgttgt ggctcctggc gggtggcctt ctgctgttcc tttccttgtc gcgaagggtc
     1861 ctggaatccc ttgccggtct gttcgggatt ttttatcccc cgtcgctcct gtttctcctt
     1921 gcgtttatat tcctgctttt gatcacgctg cattattccg tggtcctgtc cggtctgtcg
     1981 gaaaagaaca agcaactggc gcaagaggtc gcgcttctgc ggcgggagat tgaggaggcg
     2041 cttaaagggg ataaggaaaa gcgggagaca aggggttgaa agtacgttaa aatattcttt
     2101 actttgtccc tgatccatgt taaatattca cgttgaacaa tgactgcaac gaaagaggta
     2161 aggcatttat catgcaagag acagccgaaa aaatgaaccg catattca
//
LOCUS       DASSLD010000379         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_478804,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000379 DASSLD010000000
VERSION     DASSLD010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478804"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(906..1838)
                     /locus_tag="VFK23_06875"
     CDS             complement(906..1838)
                     /locus_tag="VFK23_06875"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HET7318840.1"
                     /translation="MNYRAVFVASMQTVGLYAAAFVIPVAGQILALFAPAPLIVVYVR
                     AGRREGSAALLASVLLSGLLGGWQAAAVFFFSFGLMALGAAEGMRRNMKPERVVLLGG
                     LLPMAVITLLLSSYFIRAGKNPIAAIEAYLRGSIDEAAKFYTGLGMTEMATYVSAVSD
                     TFIHYLVLLIPGITMATSVTQAACCYGLARAVIARRPDSAPVFNRQPFASWHAPDAWV
                     WGLIASLALVVVPQETARLAGWNLAIIFAVVYLAQGAAIVEHYLLKAHIKSLVRGLIM
                     ALVMAMPSIVFVIALGVVDVWADIRKVRAPFKLA"
     gene            complement(1941..2285)
                     /gene="rpsR"
                     /locus_tag="VFK23_06880"
     CDS             complement(1941..2285)
                     /gene="rpsR"
                     /locus_tag="VFK23_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET7318841.1"
                     /translation="MAEFQSRPSGPSRPSGPPRSGDRGPRGERDDRGPRAGGEKRRRF
                     QVRKKVCRFCVDKSPFIDYKDVKTLRFFITERGKILPRRISGNCARHQREVTVAIQRC
                     RNIALIPFSGEK"
     gene            complement(2342..>2609)
                     /locus_tag="VFK23_06885"
     CDS             complement(2342..>2609)
                     /locus_tag="VFK23_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET7318842.1"
                     /translation="SYIDIVVFGKQAESCGQYINKGDSVLIDGRLQQRRWETEDGQKR
                     NKIEVVAQSVNFMPKRSSGGGQGQGHGHAEPAPEAPVDEGDIPF"
BASE COUNT          585 a          712 c          731 g          581 t
ORIGIN      
        1 aacggagtca ccatgaatcc gcatacggtg aagcgtctgg cggattgcct gggcgttgac
       61 atgtcgacga ttgtaaagtg agatctctga aagctgcaac ggacaagctg cgcataacca
      121 tcattatcga agcacggtgc gcccgttctc accgggaccc tggccaggga ctgcaaagca
      181 ccggggcatt tactcagttt tcttcatcct tgataactct cccatcgacc aaatactaat
      241 ctgtcacctt ctgcgaattc tcagggtctc tctttctgcc gcaggtcaat agtattgctg
      301 tattgaatcc cggtctctcg aatttcggcg taatcagact ccttggggat tcccccccgc
      361 ctcctgtcag gaaaaagcca ttttgccgca tctgaacaaa atatgtcagt cgtacgacat
      421 aatacgtcat tgttctgcct ctcttggaca ttaaatgatc aggttattga taatttaccg
      481 ttgtctacag ctggcaaaga atatgcttat taaaatcatc cgagagaaca aaaaaccgga
      541 taaattatgg caagccgggc gctcatgaac ggcatcttgc cttctgttgg aacaattttc
      601 tacaggtctt tgggaaggag gaacgatgtc atggacaaga agggaatatc gttcatcact
      661 gaattaaaga cccaaatcag gcatgcatgc gccgactgtc atgaatacaa agtgctcgtt
      721 tatttggtta tgaatgataa gggccagatg ttcggcgtct gcagggaatg cgcgacccgt
      781 tattcgagtc tcatgcctac tgcgtagagg attgatcatc aggttatttc aataaaagat
      841 gctctcataa aaaaccgtca tctccgattg cccggaatga cggttttttt tgaaactatc
      901 cggatttacg ccagcttgaa gggcgcccgc accttgcgga tgtcggccca gacatccaca
      961 acgcccaggg ctatgacgaa aacgatggac ggcatcgcca tgacgagcgc catgatgagc
     1021 cccctgacga gcgatttgat atgtgcctta aggagatagt gctccacgat tgcagcgccc
     1081 tgggccaggt acaccacggc gaagatgatg gcaagattcc atccggcgag ccgcgcggtc
     1141 tcctggggca ctacgacgag cgcaagggac gcgatcaatc cccagaccca ggcatccggt
     1201 gcgtgccagg acgcgaaggg ttgtcggttg aagacgggtg cggaatccgg ccttcgcgcg
     1261 atgacggcgc gggccagacc gtagcagcat gccgcctgcg ttaccgacgt cgccatggta
     1321 atgcccggaa tgagaagtac gagatagtga atgaaagtat ccgagacggc gctgacgtaa
     1381 gtcgccattt ccgtcatgcc caggcccgta tagaatttcg ccgcctcgtc aatgctgccc
     1441 cggaggtatg cttcgatcgc ggctatcggg tttttgcctg cacggatgaa ataggaggaa
     1501 agaaggaggg tgattaccgc catcggcagg aggccgccta aaagaacaac gcgctcaggt
     1561 ttcatgttcc gccgcatgcc ttcggcagca cccagggcca tgaggccgaa actgaaaaag
     1621 aagacggctg cggcctgcca tcctccgagc agaccggaaa gaagaaccga cgcgaggagg
     1681 gcggccgagc cttctcgcct gccggcccgg acgtaaacga cgatgagcgg cgccggtgcg
     1741 aacagggcga gtatctggcc tgcaacggga atgacaaatg ccgctgcata gagccccacg
     1801 gtctgcatcg aggcaacgaa gaccgctcgg tagttcatgg gcatctccaa aaaaagggga
     1861 aacccgggtc ctggtgaggc aaccttgggt ttcccctgaa aaggtgtgaa agctctgtga
     1921 gggcgctcgg cccgttgcga ttatttctcg ccggaaaagg gaatgagcgc gatgtttctg
     1981 caccgttgga tcgcaacggt gacctctctt tggtgccggg cgcagttgcc ggagatgcgc
     2041 ctgggcagga tcttgccccg ctcggtaatg aagaaccgga gcgtcttgac gtccttgtaa
     2101 tcgatgaagg gcgatttgtc gacgcagaag cggcacactt tcttgcggac ctggaacctt
     2161 cgcctctttt cgccgcccgc gcgcggtccg cggtcatccc tctcgcccct cggtcctctg
     2221 tctcctgatc tcgggggacc tgaaggtctt gagggacctg atggtcgtga ttgaaactct
     2281 gccatggtag tttactcctc tagagaaatt gtttatgcgc ctgttgatca gccccgatgt
     2341 ctcaaaaggg gatatccccc tcgtccaccg gcgcctcggg cgcgggctcg gcatggccgt
     2401 gtccctgccc ctgtccgccg cctgacgagc gtttgggcat gaagttgacg gactgggcaa
     2461 ctacttctat cttgtttcgt ttctgaccgt cttcggtttc ccagcgccgc tgctggagcc
     2521 tgccgtcgat cagcacagag tctcccttgt tgatgtactg gccgcaggat tccgcctgtt
     2581 tcccgaagac gacgatatcg atgtaactg
//
LOCUS       DASSLD010000380         4820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1605920, whole genome shotgun sequence.
ACCESSION   DASSLD010000380 DASSLD010000000
VERSION     DASSLD010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605920"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..658
                     /locus_tag="VFK23_06890"
     CDS             89..658
                     /locus_tag="VFK23_06890"
                     /inference="COORDINATES: protein motif:HMM:NF024067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HET7318843.1"
                     /translation="MKALEQISFPKMAKRICALYSEDEDALRLNMLGQEYIIRHDGVY
                     IRGQKAPDVHATVLSEYLFSPGKEPIVLPWRAIGDFAAQASPDFRKKVEAPLTANATE
                     IIGRTSALFPMMDAEASSSIIGSDLAFTVHALPKVYLHVEISGETEDFPAEAWLLFSN
                     NANEFLSVQGLYALAEMFKDRLLSLIRIY"
     gene            681..1433
                     /gene="pyrF"
                     /locus_tag="VFK23_06895"
     CDS             681..1433
                     /gene="pyrF"
                     /locus_tag="VFK23_06895"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET7318844.1"
                     /translation="MTPADRLVIALDVDSDAEALGLVAELKDSVGVFKVGHQLFTAYG
                     PDIVRRITGMGGRVFLDLKYHDIPNTVAKASTEAVKLGVSIFNVHALGGLDMMNAAAD
                     AAKETAGKYGTPAPILLAVTILTSMDGPGLRRELKITRSLPRQVAHLARLAQRAGMNG
                     VVASPQELKMLRRSVRGPFIILTPGVRPAWAAKDDQRRIMTPGEAVRSGADYIVVGRP
                     VLKARDRKAAVEKILEEIAGSQESEARSQKPE"
     gene            1488..2063
                     /gene="pyrE"
                     /locus_tag="VFK23_06900"
     CDS             1488..2063
                     /gene="pyrE"
                     /locus_tag="VFK23_06900"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010638110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET7318845.1"
                     /translation="MTPEQVLDIYRKTGALLTGHFLLSSGLHSEQYLQSALVLQEPDV
                     ATRLCAALAEHFQGERVQVVIAPALGGVFVSHETARALGVRALFAERVNGELTLRRGF
                     SIKPGERVLVVEDVITTGKSTKETIRVVQQAGGVVVAAASLVDRSGGKADLGVPYRSL
                     VTLNVPTYPAEACPLCKAGSRPVKPGSRGLK"
     gene            complement(2072..3031)
                     /locus_tag="VFK23_06905"
     CDS             complement(2072..3031)
                     /locus_tag="VFK23_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7318846.1"
                     /translation="MLFPVSGVKTWVFLPPLVAFVVSSLTSMAGVSGAFILLPFQMSV
                     LGFTSPAVSSTNFVYNIVAIPSGVYRYFKEGRMAWPLTWVVIIGTLPGVFIGYYLRIL
                     YLPDPRAFKLFVGMVLLYIGGRLLYEMTGRAQASKARMGELDKKFAERARIIREQRHS
                     QTAAGLPPDAVVRTITFNMAVIEYEFWGERFRFSVPAMFLLSFVVGVIGGTYGIGGGA
                     IIAPFCVAVFHLPVYTVAGAALLGTFLTSIVGVFFYSVLPASGGLSTSPDWALGFLFG
                     VGGFAGMYVGARLQKYMPQKFIKLMLGAMLVALALRYITQYFA"
     gene            3190..4551
                     /locus_tag="VFK23_06910"
     CDS             3190..4551
                     /locus_tag="VFK23_06910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7318847.1"
                     /translation="MGKVAYTDEKNELREGVPEDVLDELADELAEEEEEEEPTAVEIA
                     EVAAEEASAGADLVDTYLKSVGRFPMLKIEEEHEYVRRLEEGKAALKSIALNSPLAIP
                     RVLALGANIKQGHVKAADVLRYPGEWNDSYEADFMRQYNRLKRQVARRDMERATATMH
                     EMSMSFREIEKMLKKLMALQKQVERGNTAELEKLGIEERSLRDMVNRIEAEDIAVKQA
                     KDELIKANLRLVVSIAKKYVNRGLTLLDLIQEGNIGLMKAADRYESGKGTKFSTYSTW
                     WIRQRITRAILDQARTIRLPVHLIEESTRISRIYTKFMREKGREPAPEEVSKIMGLSV
                     VRVNEILRAIQEPVSLETPILSEEKELKDVIIDEKSVSPFKTLENNEAANRIEQVLSS
                     LTEREEKIIRMRFGIGVGSEHTLEEVGRFFNLTRERIRQIEIKALKKLRHPARSKLLR
                     EYV"
BASE COUNT         1116 a         1348 c         1486 g          870 t
ORIGIN      
        1 tgcagccgct ctggaatgcc gatttttcaa ttggccttcc ccgcgcgtat aatttccccc
       61 atcctgctgg gtcagccgga gatcatgcgt gaaagccctc gaacaaattt cctttcccaa
      121 aatggcgaag cggatctgcg cactgtacag cgaagacgag gacgcgctgc gcctgaacat
      181 gcttggccag gaatatatca tccggcacga cggcgtctac atacgcggcc agaaggcccc
      241 tgacgtgcac gcaaccgtgc tttcggagta cctgttctct ccggggaagg agccgatcgt
      301 gctgccctgg cgggccatcg gcgattttgc cgcgcaggct tcaccggatt tcaggaaaaa
      361 ggttgaagcg cccctgaccg cgaacgcgac ggaaatcatc ggccggacat cagcgctttt
      421 cccgatgatg gatgccgagg cgtcatcaag catcatcggg agcgatcttg ccttcacggt
      481 gcacgcactg ccgaaagtgt acctgcatgt ggagatatcc ggggagaccg aggattttcc
      541 ggccgaggcc tggctcctgt tttcgaacaa tgccaacgag tttttgtccg tgcagggcct
      601 ttatgcgctt gccgagatgt tcaaggaccg gctcctgagc ctgatcagaa tatattgacg
      661 ccaccatgcg gaatgcggaa atgacaccgg ctgatcgtct cgtgatcgcg ctcgatgtgg
      721 acagtgacgc cgaagcgctc ggccttgtgg ccgagctgaa ggactcggtc ggcgtgttca
      781 aggtcggcca tcagctcttc acggcctatg gccccgacat cgtgcgccgc atcaccggca
      841 tgggcggccg ggtcttcctc gacctcaagt atcacgacat cccgaatacg gtggcaaagg
      901 catcgaccga ggccgtgaaa ctcggcgtga gcatcttcaa tgttcatgcc ctgggcggac
      961 tggacatgat gaatgccgcc gcggacgcgg cgaaggaaac ggcggggaaa tacggcaccc
     1021 ccgcgccgat tctgcttgcc gtcaccattc tcaccagcat ggacggcccg ggcctccgcc
     1081 gtgaactgaa gatcacacgc tcccttcccc ggcaggttgc ccatctggca aggctcgccc
     1141 agcgcgccgg catgaacggc gttgtcgctt cgccgcagga gctcaagatg cttcggaggt
     1201 cggtccgggg accgttcatc atccttaccc cgggcgtgcg gccggcgtgg gccgcaaagg
     1261 acgaccagag gaggatcatg acgcccggag aggcggtcag gagcggcgcc gattatatcg
     1321 tggtggggcg gccggtgctg aaagcccggg accgcaaggc ggcggtggaa aagatactgg
     1381 aagaaattgc cggaagccag gagtcagaag ccagaagcca gaagccagaa taaaggcttt
     1441 tacttcttaa ttctcacttc tgacttctct taacgaaggg gttcatcatg acaccagaac
     1501 aggtattgga catataccgg aaaaccgggg cgcttttgac cggccatttt ctgctttcat
     1561 cgggtctgca cagcgaacag tatcttcagt cggcgctggt gctgcaggaa cctgatgtcg
     1621 cgacccgcct ctgcgcggcg ctcgcggagc atttccaggg cgaacgcgtc caagtcgtga
     1681 ttgcccccgc cctcggcggc gtgttcgtgt cgcacgagac cgcgcgcgct ctcggcgtgc
     1741 gggcgctctt tgccgagcgt gtgaacgggg agttgacgct tcgtcgcgga ttttcgatca
     1801 aacccggaga gcgcgtcctc gtggtcgagg acgtgatcac gaccggaaaa tcgacgaagg
     1861 aaaccatcag ggttgtgcag caggcaggag gagtggtcgt tgctgcggcc tcgctcgtcg
     1921 accgatccgg cggcaaagcg gacctcggcg tgccgtacag atccctcgtg acgttgaacg
     1981 tgcccaccta tcctgcggag gcttgcccct tgtgcaaagc aggcagccgt cccgtcaagc
     2041 ccgggagcag gggattgaag taaccgcctt atcaggcgaa atactgcgtg atatatcgga
     2101 gcgcgagcgc cacaagcatg gcccccagca tgagcttgat aaacttctgg ggcatgtact
     2161 tctgaagacg cgcgccgaca tacatgccgg cgaaaccgcc gacgccgaag agaaacccca
     2221 gggcccagtc gggcgaggtc gaaagacccc ccgatgcagg taggacgctg tagaaaaaga
     2281 caccgacgat agaggtgagg aacgtcccga gcagggccgc tcccgcaacg gtatagacgg
     2341 ggagatggaa cacggccacg cagaagggag cgataatcgc cccgccgccg attccgtagg
     2401 tcccgccgat cacgccgacg acgaaggaca gcaggaacat cgcgggaacg ctgaaccgga
     2461 accgctcccc ccagaactca tactcgatga ccgccatatt gaaggtgatg gtcctgacca
     2521 cggcatccgg agggaggccg gccgcggtct gagaatgcct ctgttccctg atgatcctgg
     2581 ctctttccgc aaacttcttg tcaagctctc ccattcttgc cttgctcgcc tgagcccttc
     2641 ccgtcatttc atacaggagt ctccccccga tatagaggag caccatgccg acaaacaatt
     2701 tgaatgcgcg cgggtccgga aggtagagaa tccggaggta gtaaccgata aatacaccgg
     2761 gcagggtgcc aatgataacg acccaggtca ggggccaggc catccggcct tccttgaaat
     2821 aacggtagac accgctcgga atagcgacga tattatagac gaaattcgtt gagctgaccg
     2881 cagggctcgt aaatccgagc acgctcattt ggaacggcag gaggatgaat gcgccggaca
     2941 cgcccgccat ggaggtcagg gaggaaacaa cgaaggcaac cagcggcggg agaaagaccc
     3001 atgtttttac gcctgatacg gggaaaagca tgtacaggtc ttgcatgaga aggaatcata
     3061 gcacaacttg cggtgggagc gcaacggtca attattctat tatagcgcaa aaaaaatacc
     3121 gtttaagcga cagtgcgatt ttgatataat tgattgtaga aagagtggcg agcactcaat
     3181 gaggacatta tgggcaaggt agcctatacc gatgaaaaaa acgaactccg cgaaggcgtg
     3241 ccagaggacg tgctggatga actcgccgat gaactcgcgg aagaggaaga agaggaagag
     3301 cccaccgccg tcgagatcgc tgaggtcgcg gccgaagaag caagcgcagg ggcggacctc
     3361 gtcgacacct acctgaaatc cgtcggaagg ttccccatgc tcaagatcga ggaagagcat
     3421 gaatatgtgc gccggctgga ggaaggcaag gccgcgctca aatcgattgc gctgaattca
     3481 cccctggcta tcccgcgggt ccttgcactg ggcgcaaaca tcaaacaggg acacgtcaag
     3541 gccgcggacg tgcttcgcta tccgggcgaa tggaacgact cgtacgaggc ggacttcatg
     3601 cgtcagtaca accgtttgaa gcggcaggtc gcgcggcggg acatggaacg cgcgacggcg
     3661 acgatgcatg agatgagcat gtcgttccgc gagatcgaaa aaatgctgaa gaagctgatg
     3721 gcgctgcaga agcaggtcga gcggggcaac acggcggagc tggagaagct cggcatcgaa
     3781 gagcggtcat tgcgggacat ggtcaaccgc atcgaggcgg aagatatcgc cgtgaagcag
     3841 gccaaggacg agttgatcaa ggccaacctc aggctggtgg tctcgatcgc aaagaagtat
     3901 gtgaaccgag ggctgacgct cctcgacctg atccaggaag gaaatatcgg gctgatgaaa
     3961 gccgccgacc gctatgagtc gggcaaaggc accaagttca gcacctattc cacgtggtgg
     4021 atcaggcagc gcatcacgcg cgccattctg gaccaggcgc gcaccatccg tcttcccgtg
     4081 cacctgatcg aggaaagcac gcggataagc cggatatata ccaaattcat gcgtgaaaag
     4141 gggagagagc ctgcgccgga agaggtctcc aagatcatgg ggctgtccgt tgtgcgggtg
     4201 aacgagatcc ttcgcgcgat ccaggagccc gtgtccctgg agacgcccat cctctcggag
     4261 gagaaggagc ttaaggacgt aatcatcgac gaaaaatccg tttcgccgtt caagaccctg
     4321 gaaaacaacg aggcggcaaa ccgcatcgag caggtgcttt catcgctcac ggagcgggag
     4381 gagaagatca tcaggatgcg tttcggcatc ggggtgggca gcgagcacac cttggaagag
     4441 gtcgggaggt tcttcaacct cacgagggag cggatccgtc aaatcgagat caaggcgctc
     4501 aagaagctcc gccacccggc ccgcagcaag cttttacggg agtacgtctg agtccaaatc
     4561 gcgagatttc gagggagtac gattgaggaa aggcgccgaa ggaggcgcct ttttttaatt
     4621 tcagctgttc tctgcggaca agggttgacg gcctcatatc catccgcgaa tgcaatgtca
     4681 ggcagccttt ctgccgacga attcgatatc ggcaataatg cgcgttgctt catcgaggtg
     4741 gggcatgtcg agttccgggc tgctgatata tgccgccatg agcgctcggg ttttgtctac
     4801 gaggaaccag atcctccagt
//
LOCUS       DASSLD010000381         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_131406,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000381 DASSLD010000000
VERSION     DASSLD010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131406"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..993)
                     /locus_tag="VFK23_06915"
     CDS             complement(247..993)
                     /locus_tag="VFK23_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7318848.1"
                     /translation="MGSVSLLTMYIVCACVISVLPAGARLRRALLLKNTAFFAGLLLG
                     LLGIRVNVRHGDRLRRTKENALIVANHVSYIDILVIASVIPAVFITSVELGGTFFLGM
                     LARLGGSLFVERRKVTGLKQEIAEIVRVLREGHHVVLFPEGTTSNGERVHPFRNALFD
                     AAITAGADILPLCLRYASLDGGSITTENRDRLFYYGGTKFSDHFPRLLSIDSAAVEVL
                     PLPVIKVRKNASRKELALQAHDAVSNAYRG"
     gene            1210..1425
                     /locus_tag="VFK23_06920"
     CDS             1210..1425
                     /locus_tag="VFK23_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318849.1"
                     /translation="METKGNGGSAERIRIQEKALSNLADTMKKHEDQLADEIRDIQSE
                     LKALKLFLARHIPEFKKQFPEIQRKLK"
     gene            1469..1993
                     /locus_tag="VFK23_06925"
     CDS             1469..1993
                     /locus_tag="VFK23_06925"
                     /inference="COORDINATES: protein motif:HMM:NF040826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HET7318850.1"
                     /translation="MKAVAKTIKDYKLSYKKKSVSTDSYLPRGAASRSIGVCNGCGAV
                     YMNKRWYAENGKSAIAKKEKVQLTCPACLKIRDNFPGGIVTLKGDYVLPHKADLMNLI
                     RNEEERARGLNPLERVIAIKENGFGSIVISTTNEKLAQRLGRAIKKAFHGDVAYHWSH
                     DNKLVRVDWVREMD"
     gene            complement(2042..>2222)
                     /locus_tag="VFK23_06930"
     CDS             complement(2042..>2222)
                     /locus_tag="VFK23_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004215928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318851.1"
                     /translation="GIIESIIRTMKEGGRTTVIMTTHDRDQAERLADVLLLMKDGIIE
                     AQGTRDHDQQKRPIS"
BASE COUNT          538 a          567 c          669 g          448 t
ORIGIN      
        1 gcaccgccga aaagcagaaa tttttcggtg agcgtggtct tgcccgcgtc cggatggctg
       61 atgatggcga aagtccgccg tttttgtatt tcgtctgaat agtccattgt gtgcagtggt
      121 ccttgcccta tggtgttgag gtttgcggaa agctctgtaa attaacaagt ggcggctgaa
      181 atgtcaagga ttacttggga tttgatgcaa tatccggcat tcccggcaga accgcctcgc
      241 gcgagctcag ccccggtatg cgttgctcac agcgtcgtgc gcctgcagag ccagctcctt
      301 cctcgaagca ttcttgcgga ccttgatgac cgggagcggt aggacctcca ccgccgccga
      361 atcgattgac agcagccggg ggaagtggtc ggaaaatttc gtcccgccgt agtaaaagag
      421 cctgtcccga ttttccgtcg ttatgctccc gccgtccagg ctggcgtaac ggaggcagag
      481 agggaggatg tcggcgccgg ccgtgatcgc cgcatcgaaa agggcgttcc ggaatggatg
      541 gacccgttcg ccgttcgagg tggtgccttc cgggaaaagg acaacatgat ggccttcccg
      601 cagcacgcgc acaatctccg cgatctcctg tttcagtccc gtgacttttc tccgctcgac
      661 gaacaggctc ccgccgagac gcgcaagcat gccgagaaag aaggtgccac cgagctcgac
      721 cgaggtgata aagaccgccg ggatgaccga tgcgatgacg agaatatcga tgtacgaaac
      781 gtggtttgca acgataagtg cgttttcttt ggttctccgt agccggtcgc cgtgcctgac
      841 gttgacccgg atgccgagaa gcccgagcag gagcccggcg aagaaggccg tatttttaag
      901 tagaagcgcg cgacgcaggc gggcgcccgc gggaaggacg ctgatgacgc atgcgcagac
      961 gatatacatc gtgaggagag agacgctccc catccatttg aacagaactc gcatagggaa
     1021 tagataatac cgccggtccg cgacgcagcg tcttgtaacg tgaagcggga agccggcggc
     1081 agattatcgc acaggaaggc gcggttttca acgggaaaac aacctttttt tgccgacttt
     1141 acttcggttc ggagcggggt tatactttaa gtaaggatga gtgttgaata tccaaaaggg
     1201 aggtgaatta tggaaacaaa agggaatggc ggcagcgcgg aacgcatcag gatacaggaa
     1261 aaggccctgt cgaatctggc cgacacgatg aaaaagcacg aagatcagct cgccgacgag
     1321 atcagggaca tccaatccga gctgaaagca ctcaaactgt tcctcgcgcg gcacattccc
     1381 gagttcaaga agcagtttcc cgagatccag agaaagctga aataagcccc taccggggcg
     1441 gtttctccgt gaccaaggag gcgattctat gaaggcagtc gcgaagacga tcaaagatta
     1501 caagctgagc tacaagaaga aatcggtttc gacagattca tatcttcccc gcggcgcggc
     1561 aagccgcagc atcggcgtat gcaacgggtg cggcgccgta tacatgaaca aacgatggta
     1621 tgcggagaat ggaaaatcgg caattgccaa aaaggaaaaa gttcaactga cgtgcccggc
     1681 ctgtctcaag attcgcgaca atttcccggg cggcatcgtg acgctgaaag gcgattatgt
     1741 gctcccgcac aaagcggacc tgatgaacct catccggaac gaagaggagc gggcccgcgg
     1801 actcaatccc ttggagcgcg tgatcgccat caaggaaaac ggcttcggga gcattgtcat
     1861 ctcaacgacg aacgagaagc ttgcgcagcg gcttggaagg gcgatcaaga aggcgttcca
     1921 cggcgatgtg gcgtatcact ggtcgcacga taacaaactg gtgcgcgtcg attgggtgag
     1981 agagatggat taaggttaga ttttaacgag tgaagaagaa ggacgagagg gctgatttcg
     2041 attacgaaat cggccttttt tgttggtcgt gatcccgtgt cccctgcgct tcaataattc
     2101 cgtccttcat gagaagcagt acatccgcga ggcgctcagc ctggtcgcgg tcatgggtgg
     2161 tcatgatgac ggtcgtgcga ccaccttctt tcatcgtcct gatgatggac tcgatgatcc
     2221 cg
//
LOCUS       DASSLD010000382         4597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1580343, whole genome shotgun sequence.
ACCESSION   DASSLD010000382 DASSLD010000000
VERSION     DASSLD010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580343"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VFK23_06935"
     CDS             complement(<1..1150)
                     /locus_tag="VFK23_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HET7318852.1"
                     /translation="MKPHGKTFWIVLVIMIVGGLSEIAVAGTKVFEVDREFYPYYPSL
                     IKWNKSNAPFTPPEACRGCHPKQYNDWTGSVHAMSFQDPIFQGELNKAVQAAGPNVAR
                     QCVGCHSPAGMVTGEIKKPGIAGLSPMALAGVSCDICHSISEVTHLQTPSHEPENGSL
                     ILSPGVDSIAGVKLIKRGPFKPSDDCGKGFHECAETPVLLRAELCAGCHQVYHYDVHF
                     PLGSTYLEWKHSAYAQKEILCQDCHMVDFGVFLSAADDFRKPERDEYRHFFNGANYLL
                     YYLAATAAKKAGDEARAATIMYKYDMAVKRLKSAADLEISPFYRNGKISEVRVRVKNI
                     RAGHNLPTSMTNIRQMWLEITIKDDRGNILLSSGSVDAEGRLPENTRMF"
     gene            complement(1577..3058)
                     /locus_tag="VFK23_06940"
     CDS             complement(1577..3058)
                     /locus_tag="VFK23_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318853.1"
                     /translation="MKRIAIIVTVFTVVAATSLLLLASSASAVPSFSRKFGAECSMCH
                     TMWGALNGAGVTFRLSGYRATNGKDLEPVEKNVELPPSNVVIPSSFPLSIITGVGYDA
                     VKTKREAADGTTTTRTGTSLALEDASIFLTGPLGEHLSAFAEFPMFETRNGEFLPTGP
                     AEANDKKTGAIQFSSEKPTFEVAKFWWNNLLGEMAPRDSVNLLGGITQLPLGYASGKV
                     RLSVNQYPIYERRALDLISPKRPDDFITDDMGLFRLSEAQVLFEVNGMVIPGKAVTDV
                     GKKDTFWFEYHAGITNGSEEKADNNNQKDLYGRFVMRWYRQSLGFFVYDSPDTYSNDI
                     REAGQTAGVMSGRQSHNGATRIGIDATLSLAAIDIPVWLENQYMTNKEDDPTGYGVAF
                     KWQGGFDQLNWQISKKEIVYARYDWIKGDSYDDTTKSVNNVPGITKSKPSETDYVVGG
                     QYLVQQNVKLIAEYRHDEFKDTATTPNSKITSDGYTVRCMIGF"
     gene            complement(3592..>4597)
                     /locus_tag="VFK23_06945"
     CDS             complement(3592..>4597)
                     /locus_tag="VFK23_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7318854.1"
                     /translation="ANIAARYSFGFAGGLMTAYGLIAYARKVSDLSRSVSRNLHYAGI
                     TFVFYGVFAGIFSFSYTLPLTPVPIVAFRGVAAIFVTYFIIKALNIFDIEMRMKYEQQ
                     TKAIIQAEKLSSLGRLAAGTAHEINNPLTNASLSVQMMKNKLRGGESEREFAEKLNAV
                     ERNIDRASLIARELLQFSRQRDTTLAPLNINNVIASSLTLLRHRLGRVELTQNLAEVP
                     DVLGDAVKLEQVFINILENALDAMPEGGRLSISTERNNGMVESRVTDSGGGIPEENLS
                     RVFDPFFTTKEIGQGTGLGLAISYGIIKQHHGEISIVNVPTPGTMVTIKLPVKEDSSG
                     "
BASE COUNT          961 a         1299 c         1259 g         1078 t
ORIGIN      
        1 taaacatgcg ggtattttct ggcagacgac cttcggcatc aactgaaccg cttgacaaaa
       61 ggatgttccc tctatcgtct tttatagtga tctccagcca catttggcga atattggtca
      121 tggaggtagg cagattgtgg ccggcccgga tgttcttgac tcgcactcga acctcactga
      181 tctttccatt tcggtaaaaa ggagatatct caagatcggc agccgacttg agccgcttta
      241 cagccatatc atatttatac atgatggttg cggctcgcgc ctcatcgccg gctttttttg
      301 cggcagtcgc cgcgagataa tagagaaggt aatttgcgcc gttaaagaaa tggcggtatt
      361 catcgcgctc gggtttccgg aaatcatcag cagcgcttaa aaaaacaccg aaatcaacca
      421 tgtgacagtc ctggcaaaga atctccttct gagcgtaggc gctgtgcttc cactcaagat
      481 atgtggatcc taacgggaaa tgaacgtcat aatgatagac ttgatggcac cctgcgcaaa
      541 gctcggccct caagagcacc ggggtttcgg cgcattcatg aaaacctttt ccgcaatcat
      601 ccgacggctt gaaaggcccg cgcttgatga gtttgacacc cgcgatggaa tccactccgg
      661 gggagaggat gagcgagccg ttttcaggct cgtgggaggg ggtctgcaag tgagttactt
      721 cgctgataga atggcaaata tcacacgata cgccggccaa cgccatgggg ctcaggcccg
      781 cgatgcccgg tttcttaatc tcgccggtga ccatcccggc cggtgaatgg caacccacgc
      841 actgacgggc gacattgggt cccgcagcct gaacggcctt gttcaactcc ccctggaaaa
      901 taggatcctg aaatgacatc gcatgcactg accctgtcca gtcattgtac tgtttcggat
      961 ggcagcctct gcatgcctca ggcggagtaa acggcgcgtt ggacttgttc cacttgatca
     1021 aggagggata gtaagggtag aattccctat cgacctcaaa aacctttgtg cccgcgacgg
     1081 caatctctga taaaccgccc acgatcataa tcacgagcac aatccaaaac gtctttccat
     1141 gcggtttcat aaaacctcct gagaccactc atattcgtcg attgaggccg gtatcaccta
     1201 ttttttttca gcatcagtcg tttttgagga aggcccgtgc gtactcaaaa gataatccag
     1261 cagggcatcc tttccccgtt tcggcacctc ggccccctgg tcgatcatcc gctcgataac
     1321 gtctttccat tcctccttag ttttatgaat gttgtcgagc ggccgtatct tggtagagtg
     1381 acacggtaaa cacgattcct gaaaaatctt ccctccgtcc tccccgtgcg cgagagcagg
     1441 tgctgcaatt gcgaagaatg ccgcgcacag agcccatgaa aacatgcgaa ctgtctgcat
     1501 agttatccct tttgtcaaat aaaaacagcc gagcaagagg cggacctccc acccggcctg
     1561 ccggctgccc ggtgcatcag aatccgatca tgcagcggac tgtgtaccca tcgctggtga
     1621 ttttcgagtt cggcgtcgtt gcggtatcct tgaattcatc gtgccggtac tcggcgatca
     1681 gtttgacatt ctgctgcacg agatactggc cgccgacgac atagtccgtc tcgctcggct
     1741 tcgatttcgt gatgcctgga acattgttca cggacttcgt cgtgtcgtcg tagctgtcgc
     1801 ccttgatcca gtcgtacctg gcgtaaacga tttccttctt cgagatctgc cagttgagct
     1861 gatcgaaccc gccctgccac ttgaaagcga cgccgtatcc cgtggggtcg tcttccttgt
     1921 tcgtcatgta ctggttctca agccacacgg gtatatcgat tgccgcgagg gagagtgtgg
     1981 cgtcgatgcc gatcctcgtg gcgccgttgt gggactgcct ccccgacatg acgccggccg
     2041 tctggcctgc ctccctgatg tcgttgctgt aggtgtcggg actgtcgtag acgaaaaagc
     2101 cgagcgactg ccggtaccag cgcatgacga agcggccgta caggtctttc tggttgttgt
     2161 tgtccgcctt ctcctcgctg ccgttcgtga tgcccgcgtg gtactcgaac cagaaggtgt
     2221 ccttcttccc cacgtcagtg accgcctttc cgggaataac catgccgttc acctcgaaca
     2281 ggacctgagc ttcgctcagc ctgaacaggc ccatatcgtc ggtgatgaag tcatcgggtc
     2341 tcttcgggct gataaggtcg agggcccgcc gctcgtagat tgggtactgg ttgacggaaa
     2401 gccgcacctt gcccgaggcg tagcccagcg gaagctgggt tatgccgccc aaaaggttca
     2461 cgctgtcacg cggcgccatt tcgcccagaa ggttgttcca ccagaacttc gctacctcga
     2521 aggttggctt ctcggatgag aattgaatgg ccccggtctt cttgtcgttg gcttcggccg
     2581 gacccgtggg caggaattcc ccgttcctgg tctcgaacat cgggaactcg gcgaacgccg
     2641 acaggtgctc gcccagcggc ccggtcaaaa agatgctggc gtcctcgagg gcgaggcttg
     2701 tgccggtccg cgtcgtcgtg gtcccgtctg cggcctcgcg cttcgtcttc acggcgtcgt
     2761 agcccacgcc cgtgatgatc gacaagggaa aggacgaggg gatgacgacg ttgctcgggg
     2821 gaagttcaac gttcttctca acgggctcga ggtccttccc gttcgtcgcg cggtagccgg
     2881 agagcctgaa ggtgacgccg gccccgttca gcgcgcccca catggtatgg cacatggagc
     2941 actccgcgcc gaacttgcgc gaaaacgacg gcactgccga cgcgctcgat gcgagcagca
     3001 agagcgatgt tgcagctact acggtgaaga cagttacgat gatggcaatc cgtttcatga
     3061 catctcctct cttcgatttt gatgatgctt tcttaatctg cattattctt catgtttcgt
     3121 aatctgcata tcaggcaaaa aagtttccca cggagtcttc tgttttcctc aacccccttt
     3181 ctcttatagt ctcccgcgat cgcgaaaccg gaaaacggac atacacaatc cagcatgaaa
     3241 aaaccggacg ccttatttaa agaatgcccg tgaatgggga caggtcgatg aacacctgga
     3301 cttttcagtc aattcacggg caaagcttaa gacatgcgat tctctcattt tagcgcaacc
     3361 agacggcaaa tttgcagaac cctctatttc ttaaactgta cgctcaatcg cacggcgaat
     3421 acaagagaag atctgttcac attgtggcgt tttgtgtgag aatttgttta catcaagata
     3481 ggggcgtctc ctgccgtctt ttacgcaaac agcagatcaa caccggtatg ccataaggcc
     3541 ggcgggacga caatagtcgc tgtgaacttg gcttgacgag cgctgcctag attatcctga
     3601 gctgtcttcc tttacaggaa gtttgatcgt caccatcgtc cccggagtcg gcacgttgac
     3661 gatgctgatc tcgccgtggt gctgtttgat gatcccgtag ctgatggcga ggccgagccc
     3721 cgtgccctgg ccgatctcct tggtcgtaaa aaagggatcg aaaacgcgcg acagattttc
     3781 ttcggggatg ccgccgcccg agtccgtcac ccgcgattcg accattccgt tgttccgctc
     3841 cgtggatatc gacaggcggc ccccctccgg catggcatcc agggcgttct caagtatgtt
     3901 gatgaatacc tgctccagtt tcacggcatc gcccagaacg tccggcacct ctgccaggtt
     3961 ctgggtgagc tcgacccggc cgagcctgtg gcggagaagc gtcagcgagg aggctatgac
     4021 gttgttgatg ttcagggggg cgagcgtcgt gtcccgctgc cgcgaaaact gtaacagctc
     4081 acgggcgatc agcgatgccc ggtctatgtt gcgctcaacg gcgttcagtt tctccgcgaa
     4141 ctcgcgttcg gactctcccc cccggagttt gtttttcatc atttgaacgc tgagggacgc
     4201 attggtcagg ggattgttga tctcatgcgc ggtgccggcg gcaagccggc cgagcgagga
     4261 cagcttctcg gcctggatga tcgctttcgt ctgctgctcg tatttcatcc gcatctcgat
     4321 gtcgaaaatg ttcagggcct tgatgatgaa gtaggttaca aatatggccg caacaccccg
     4381 gaacgcaacg atggggaccg gcgtcagcgg cagcgtgtac gagaaggaga atatccccgc
     4441 gaatacgccg tagaaaacga aggtaatgcc cgcgtagtgc aggttacgcg acaccgaacg
     4501 gctgaggtcg ctcactttgc gcgcataggc gatgaggccg taggcggtca tgagtccgcc
     4561 cgcgaagccg aaggaataac gggccgcgat gttcgcc
//
LOCUS       DASSLD010000383         7035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1181125, whole genome shotgun sequence.
ACCESSION   DASSLD010000383 DASSLD010000000
VERSION     DASSLD010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181125"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..198)
                     /locus_tag="VFK23_06950"
     CDS             complement(76..198)
                     /locus_tag="VFK23_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318855.1"
                     /translation="MMSRERILLLLLIVIVALLAVTAIKPYDRGTWLMEVARSS"
     gene            complement(305..1342)
                     /locus_tag="VFK23_06955"
     CDS             complement(305..1342)
                     /locus_tag="VFK23_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HET7318856.1"
                     /translation="MNPVKDLKISLLMLFALIAAGTAGYMTIEGWNFLDALYMTVITI
                     GTVGFQEVHRLSDAGRVFTIVMIVVGVMVLGYTVGKLAQIMFEGQFQRFLGRKKVEKS
                     IGELRDHYIVCGYGRIGALICREFAAKPVPFVVVEHSPAVLEKLEEDGVLFLRGNATE
                     DETLLKAGIKRAKGLVSVVTSDTENVYITLTARGLNPDLYILARSGEPGSDIKLTRAG
                     ANKVVSPYQIGGNRMAQAILRPNVVDFIEIAMGREHLDLQMEEIFIPGHSPIIGLNLM
                     EAGLRRDTGVIIVGIKKEAGKMVFNPGGTSRLDARDTLIVLGQPTEIMKLEKLVECNA
                     PAGERGVGQND"
     gene            complement(1494..2795)
                     /locus_tag="VFK23_06960"
     CDS             complement(1494..2795)
                     /locus_tag="VFK23_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET7318857.1"
                     /translation="MKTYFEFARHRTGYKQETLAGIATFLTMAYIVVVNPAVLEAAGI
                     PKGPSMTATILSAAFGTLVMGVYAKRPFAIAPYMGENAFIAFTVVRVLGLPWQTALGA
                     IFVAGALFSVLTVTRVRGWLADTLPQSLKHSFSVGIGLFLAFIGLNLTGIVTLGVPGA
                     PVGLGRLSEPPVVLAVSGFFLIIWLMIKGVRGAVIIGILAVTLLSFFLGITPAPKSLL
                     SLPPGLGPIAFQVNVMDALSLKNLPIALIIFVMAFVDTVGTLIGLSARAGLLDEKGNL
                     PAIEKPMLADALANLVAPLLGTTTTGAYIESAAGITEGGRTGFTAVVVAFLFLLTLFL
                     APVLTAVPPHAYGTALVVIGVFMLGPLRSIDFSDHTELVPAFLTIVMMIFTFNIGVGM
                     TAGLLAWPVLKTVSGRRAEVRAGMWVFAAFSLLFYIFYPYR"
     gene            3097..3528
                     /locus_tag="VFK23_06965"
     CDS             3097..3528
                     /locus_tag="VFK23_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET7318858.1"
                     /translation="MKVEQISIFLENKSGRLADVTDVLAKNGINIRALSLADTVDFGI
                     LRLIVNDLEKTKKLLKDSGFTVGQNEVVAVEMPDQPGGLAGILAILQKSGVNVEYMYA
                     FIQKCEGKAVLIFRFDEIDKAIDVLQKAGIRILKREEVCAL"
     gene            3581..4726
                     /locus_tag="VFK23_06970"
     CDS             3581..4726
                     /locus_tag="VFK23_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7318859.1"
                     /translation="MRLLKLVAVAACIIALSVAPAMAAGTIKVGAILSVTGPASFLGA
                     PEAKTLEMLVSDINAKGGVNGTKIELIVKDSGGSPEKAVSLAKQLIDEEKVFAILGPS
                     TSGETMAIKNLAEESKTILLSCAAAEAIVNPVAKYVFKTPQKDSDAAIKIFQEMQKRK
                     ISRIGLLSSNTGFGNAGKGQIEKLAPSFGVTILANEVYDKAASDLTAEVTKIKAANVQ
                     AIINWSIEPAQAIVIKNARQIGLTVPIFQSHGFGNIKYVEAAGAAAEGVIFPAGRLLV
                     ADKLSNKNPQKPVLLSYKKAYEAKYKDEASTFGGHAYDALLILTKGIEKANSTDREAV
                     RTAIENLTDVVGTGGIFYFSPSDHNGLNVDAFELLTVKNGKFAVLGK"
     gene            4859..6001
                     /locus_tag="VFK23_06975"
     CDS             4859..6001
                     /locus_tag="VFK23_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011189435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7318860.1"
                     /translation="MRFLKFLILSFFLPVLSLTQAFAEDTLKVGAILSVTGPASNLGA
                     PEARTLSMLVEDINARGGIIGKKVELVVKDSGGSPEKAVSFAKQLIEEEKVFAMIGPS
                     TSGETMAIKNIAEEGKTILLSCAAAEVIVSPVAKYVFKVAPKDGFAAVRIFDEMKKLG
                     ISKIGMLSSNTGFGKAGKEQLEKLAPEHGIQIAISEVYDKSATALTAEVTKLKAKGVQ
                     AIVNWSIEPAQSIVIKNARQTGMTIPIFQSHGFANIQYARTAGSAAEGVIFPASRIVV
                     SDALPEKNPQKPVIMAYKKAYEAKYHEDVSTFGGHAYDAMMILARAIREAGADREKVR
                     SAVENMKNFIGTAGVFNFSPTDHNGLDINAFAMLTVKDGRFVLLER"
     gene            6131..7018
                     /locus_tag="VFK23_06980"
     CDS             6131..7018
                     /locus_tag="VFK23_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7318861.1"
                     /translation="MIELFFQYLVAGLTYGTIYAIVGIGFNIIYNATGIINFAQGEFV
                     MLGGMTAVTLNGLMPLPLAILCAVAVTMAVGGLIEMTFIRWLVKPSVLRMIIITIGIS
                     ILIREAALLIWGEGVRSLPYFTGNEISAISVGGVRVSPQVIWSIGVCSIVVLLLNLFF
                     KHTMLGREMRACAANREAAALCGIPTKNMVTLSFVLSAGIGALAGCVVSPITYTQYNI
                     GTGLAIKGFTVAILGGLGNSMAAVGAGFILGVLESFSIWVLPTAYKDVISISILLAML
                     FVRPSGLFGNKEAMRLKDF"
BASE COUNT         1551 a         2087 c         2089 g         1308 t
ORIGIN      
        1 ggtaagcagg gcaacgattg ccccgatgcg atgtgagggg aaacctccgg taggtgagga
       61 ccagcagcgg caaggctatg aggaccgcgc cacctccatt agccaggtgc cgcggtcata
      121 gggtttgatt gcggtaaccg ccaggagagc gacgataacg ataagcagca ggaggagaat
      181 tcgttcacgt gacatcattg cctgctcctt tccgaatact aagggtgggg cccctcaggc
      241 gggagccgga ggctccaaaa taataacgca ccatcccgca gctcggaaac tgtcatgtgc
      301 cgcttcaatc gttctgtccc acacccctct ccccggccgg cgcgttgcac tcgacgagtt
      361 tctcaagctt catgatttcc gtgggctggc ccaggacgat gagcgtgtcc cgggcgtcga
      421 gcctgctcgt cccgcccggg ttgaagacca tcttgccggc ttccttcttg atgcccacga
      481 tgataacgcc cgtgtcccgg cgcaggcccg cctccatcag gttgaggccg ataatgggcg
      541 aatggccggg tatgaagatt tcctccatct gcaggtcgag gtgctcgcgc cccatggcga
      601 tctcgatgaa gtcgacgacg ttcggacgga ggatcgcctg ggccatgcgg ttccctccga
      661 tctggtaggg ggagacgacc ttgttcgcgc ccgcgcgtgt gagcttgatg tccgagcccg
      721 gctcgcccga gcgggcgagg atatagaggt ccgggttcag tcccctggcc gtcaaggtga
      781 tgtacacgtt ctccgtgtcc gacgtgacaa cggagacaag ccctttggca cgcttgatcc
      841 ccgccttgag cagcgtctcg tcctccgtgg cgttgccgcg caggaaaagc acgccgtcct
      901 cctccagctt ttcgagcaca gcgggactat gctccaccac cacgaagggg accggcttgg
      961 ccgcgaattc ccggcagatg agcgcgccga tcctcccgta cccgcagacg atatagtggt
     1021 cccgcaactc cccgatcgac ttttccactt ttttcctccc aagaaaacgc tggaactggc
     1081 cctcgaacat gatctgcgcc agcttgccca cggtgtaccc caggaccatc acgccgacga
     1141 cgatcatgac aatggtgaac acccggccgg cgtcgctcag gcggtgcact tcctggaagc
     1201 ccacggtgcc gatggtgatg accgtcatat agagggcgtc aagaaaattc cagccttcta
     1261 tggtcatgta ccccgccgtg cctgccgcga tgagcgcgaa gagcatgaga agcgatattt
     1321 tcagatcctt gaccgggttc atagaagtaa tatcacttaa taaaatttca ttcccgcaac
     1381 gcttctgagc tggaatccat tttatcgtat caaagtggat cccgatagag gcattggggg
     1441 ataataatct tttttagtat ttgtcatctc ccaaaatccg cgtccccgtg acgtcaccgg
     1501 tagggataaa aaatgtagaa caggagcgaa aacgcggcga agacccacat gcccgcgcgg
     1561 acctctgcgc gcctgccgct tacagtcttc aagaccggcc aggcaagcaa gcccgcggtc
     1621 atccctacgc cgatgttgaa cgtgaagatc atcatcacga tcgtcagaaa ggcggggacc
     1681 agctcggtat ggtcgctgaa gtcgattgac cggagcgggc cgagcatgaa gacgccgata
     1741 accacgagcg ccgtgccgta ggcatgaggc ggaaccgcgg tcaggaccgg cgcgagaaac
     1801 agcgtcagga ggaagaggaa cgcgaccacg accgcggtaa agccggtacg gccaccctcg
     1861 gtgatgcccg ccgcggactc gatgtaggcc cccgtcgtcg tagtccccag aagcggcgcg
     1921 accaggttcg cgagcgcatc ggcgagcatg ggcttctcga tcgccggcag gttccccttt
     1981 tcatccagaa gcccggcgcg cgcggagagc ccgatcaagg tccccacggt gtccacgaag
     2041 gccatcacga aaatgatgag ggcgatgggc aggttcttga gggagagcgc gtccatgacg
     2101 ttcacctgga aggcgatagg gccgaggccc ggcggcaggc tcagcaggga tttcggcgcc
     2161 ggcgtaatgc cgaggaaaaa agagaggagc gttacggcga gaatgccgat gatgaccgcg
     2221 cccctgaccc ctttgatcat gagccagatg atgaggaaga acccggatac ggcgagcacg
     2281 accgggggct ccgaaagcct gcccaggccc acgggcgcgc cgggcacgcc caaggtcacg
     2341 atcccggtca ggttcaggcc gatgaaggcg agaaagagcc cgatgcccac ggagaagcta
     2401 tgcttgagcg actgcgggag cgtgtcggca agccatcccc gcaccctggt cacggtcagc
     2461 acggagaaga gggccccggc gacgaagatt gcgcccagcg ccgtctgcca ggggagcccc
     2521 agcaccctga ccacggtgaa ggcgatgaag gcgttctcgc ccatgtacgg cgcgatcgcg
     2581 aagggcctct ttgcgtaaac gcccatcacg agcgtgccga aggccgccga aaggatcgtg
     2641 gcggtcatgg acgggccttt gggaatgccc gcggcttcga gaacagcggg gttcaccacg
     2701 acgatatagg ccatggtcag gaacgtggca atgccggcga gcgtttcctg cttgtagccg
     2761 gtgcggtgtc tggcgaattc aaagtaggtt ttcatggtga taaatagagc ggccgctcag
     2821 atccgttaga gaaaaagcat ctcgcacaaa gacgccaagg acgcaaagaa aacaagtccg
     2881 ttgtttttta aaaaccttca gggcttcagt ctctctttgc gcgctttgca agagaacgat
     2941 cgtcacaaaa atagtataaa ttaccggaaa aggcaaggcg aaaccgcatg agctttacct
     3001 tgacaaagac acgcgaatcg acatattatt atgcgtttca ccggaaaacc cggccacgcg
     3061 ccgcgcacct cgagctccgg ccggacggga gggcccatga aggtcgagca gatatccata
     3121 tttctcgaga acaaatcagg gcggctggct gatgtgaccg acgtccttgc caaaaacggg
     3181 atcaacatcc gggcgctctc actggccgac accgttgatt tcgggatcct gcggctcatc
     3241 gtaaacgatc ttgagaaaac gaaaaagctc ctcaaggaca gcggctttac tgtgggccaa
     3301 aatgaggtgg tagcggtgga aatgcccgac caacccggcg gcctcgccgg gatacttgct
     3361 attttgcaga agagcggcgt taacgtcgaa tatatgtacg ccttcatcca gaaatgcgag
     3421 ggcaaggccg tcttgatctt caggttcgac gaaatcgaca aggccatcga tgtcctccaa
     3481 aaggccggca tccggatcct gaaacgcgaa gaggtctgtg cactctagga accatcccct
     3541 cggacacggg ggacatcata cttcaggagg aagagaacct atgagattgt tgaaactggt
     3601 ggcagttgca gcatgtatca tcgccctgtc cgttgctccg gcaatggcgg cgggcacgat
     3661 caaggtcggc gcgatcctgt ccgtgaccgg gcctgcatcg tttctcggcg cgcctgaggc
     3721 aaaaacgctg gagatgctcg tctcggacat caatgccaaa ggcggcgtga acggcaccaa
     3781 gatcgagctg atcgtcaagg actcgggcgg ctctcccgag aaggcggttt ccttagccaa
     3841 gcagttgatc gatgaagaga aggtctttgc catcctaggg ccttcaacct ccggcgagac
     3901 catggcaatc aagaaccttg ccgaggagag caagaccatc ctgctctcct gcgcggcggc
     3961 cgaggcgatc gtcaatcccg tcgcgaagta cgtgtttaag acgccccaga aggacagtga
     4021 cgcggccatc aaaatcttcc aggagatgca gaagagaaag atatccagga tcggtctcct
     4081 ttcgagcaac accggcttcg gaaacgcggg gaagggacaa atcgagaagc tcgctccgtc
     4141 attcggtgtc acaatcctcg ccaacgaagt atatgataag gcagccagcg acctgacggc
     4201 cgaggtgacc aaaatcaagg ccgccaacgt ccaggcgatc atcaactggt ctatcgagcc
     4261 agcgcaggcg atcgtgatca agaacgcgcg ccagatcggc ctcacggtgc ccatcttcca
     4321 gagccacggg ttcgggaaca tcaagtacgt cgaggccgcc ggcgcggctg cggagggcgt
     4381 catcttcccg gcagggagac tcctcgtagc agacaaactt tcgaataaaa acccgcagaa
     4441 acctgtgctt ctgtcgtata agaaggccta cgaggccaag tacaaggatg aggcgagcac
     4501 cttcggtggt cacgcctacg acgcccttct catccttacg aaggggatcg agaaggccaa
     4561 cagcacggac agggaggctg tccggaccgc catcgagaac cttacagacg tcgtcgggac
     4621 cggaggaatc ttctacttct ccccgtcgga ccacaacgga cttaacgtcg acgccttcga
     4681 gctgctgacc gtgaagaacg ggaagttcgc ggtactcgga aagtagcctg ttccaagacc
     4741 tgcgcaggga gtgtccgaaa acggacactc cctgttttat catttcaccc gacgtgccgg
     4801 caagccatgc agtcgccggt ctgatcgcaa ctggtcggac cggagaggag attacctcat
     4861 gaggtttttg aagtttctta ttctgtcgtt ctttcttccg gtcctgtccc tcacgcaggc
     4921 gtttgccgag gacacgctca aggtgggggc catcctgtcc gtgaccgggc cggcatccaa
     4981 cctgggcgcg cccgaggcga ggaccctctc catgctcgtg gaggacatca acgccagggg
     5041 aggcatcatc ggcaagaagg tcgaactggt ggtcaaggac tccggcggct cgccggaaaa
     5101 ggcggtctcc ttcgccaagc aactgatcga ggaggagaag gtcttcgcaa tgatcgggcc
     5161 ttcgacctcc ggcgagacca tggccatcaa gaacatcgcc gaagagggaa agaccattct
     5221 gctttcctgt gcggcagcgg aagtgatcgt gagccccgtg gcaaagtacg tcttcaaggt
     5281 ggcccccaag gacggctttg cggccgtcag gattttcgac gagatgaaga aactgggcat
     5341 ctcgaagatc ggcatgctgt cctccaacac gggcttcggc aaggcgggca aggagcagct
     5401 tgaaaagctc gcgcccgagc acggcatcca gatcgcgatc agcgaggtct acgacaagtc
     5461 ggccacggcc ctgaccgccg aagtgaccaa gctcaaggca aagggagtgc aggccatcgt
     5521 gaattggtcc atcgaaccgg ctcagtccat cgtgatcaag aacgcccggc agaccggcat
     5581 gaccattccc atcttccaga gccacggctt cgccaacatc cagtatgcca ggaccgcggg
     5641 gagcgccgcc gagggcgtca tcttcccggc aagcaggatc gtggtgtccg atgcgctgcc
     5701 ggaaaagaat ccgcagaagc cggtcatcat ggcgtacaag aaggcttacg aggcgaaata
     5761 tcatgaggac gtcagcacct tcggcggaca cgcctatgat gcgatgatga tcctcgcccg
     5821 ggccatccgc gaggcgggcg ccgaccgcga gaaggtccgg agcgcagtcg aaaacatgaa
     5881 gaacttcatc ggcacagccg gggtgttcaa tttctcgccc accgatcaca acggcctcga
     5941 catcaacgcc tttgccatgc tgacggtgaa ggacggcagg ttcgtgctgc tggagagata
     6001 acttccggaa ggtgggaggt tgggaaggta ggagggtagg atcaggaagg aagaacatgg
     6061 attgagtcgc ttcccacctt ctcaccttcc taccccctca ccttcatttt tttgcaattg
     6121 aggtgtcttt ttgatcgagc tttttttcca gtatctcgta gccggcctca cctacggcac
     6181 catctacgcc atcgtcggca tcgggtttaa tatcatctac aacgccaccg ggatcatcaa
     6241 cttcgcccag ggagaattcg tcatgctcgg cggcatgacc gccgtgacgc tcaacggcct
     6301 catgcccctt ccgctcgcaa tcctctgcgc tgtcgcggtc accatggccg taggcgggct
     6361 gatcgaaatg accttcatcc gctggctcgt gaagccgtcg gtcctcagga tgatcatcat
     6421 cacgatcggc atctcgatcc tgatccgcga ggcggcgctc ctgatctggg gcgagggcgt
     6481 ccgctccctg ccctacttca cgggaaacga gatctcggcc atctcggtgg gcggcgtccg
     6541 cgtttcgcct caggtcatct ggagcatcgg cgtctgctcg atcgtcgtcc ttctgctcaa
     6601 cctctttttc aagcatacga tgctgggccg ggagatgcgg gcctgcgccg cgaaccgcga
     6661 ggccgcggcc ctctgcggca tcccgacaaa aaacatggtc accttgtcct tcgtactctc
     6721 tgcagggatc ggcgccctgg cgggctgcgt cgtgtcgccg atcacctata cgcagtacaa
     6781 catcggcacc ggcctcgcca tcaagggatt caccgtcgcc atcctcgggg gcctgggcaa
     6841 cagcatggcc gccgtaggcg cggggttcat cctcggcgtc ctcgagtcct tcagcatctg
     6901 ggtcctgccg acagcttaca aggacgtgat ctccatctcc atcctgctcg ccatgctctt
     6961 cgtcaggccg agcgggttgt ttggaaacaa agaagccatg cggctgaagg atttttgatg
     7021 aagattcctc gattt
//
LOCUS       DASSLD010000384         3469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1305930, whole genome shotgun sequence.
ACCESSION   DASSLD010000384 DASSLD010000000
VERSION     DASSLD010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305930"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFK23_06985"
     CDS             complement(<1..294)
                     /locus_tag="VFK23_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7318862.1"
                     /translation="METIISVSCLKHTYPDTTEIHICGLDFVVNRGERVAILGGNGSG
                     KTTLLYHILGLLKPDEGRVSVFGVNPAQRYNEIRKRIGVLLQNVDDQILSPTVW"
     gene            complement(323..1030)
                     /locus_tag="VFK23_06990"
     CDS             complement(323..1030)
                     /locus_tag="VFK23_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HET7318863.1"
                     /translation="MIDVARIDYWAASGSGAFHRASVLAKLIFVAFVVAAAVLSRSVP
                     ALAAGYALLLAASAAARLPLLRIAMLSLYAAVFAALYGISLRGVGLAVYGLVILKAVT
                     PALAVLMLIVSTPYPRLFSLISAVLPELPAAGLFMTYRTFFILLDMMNNFIVALRLRA
                     GLARGRIVKTIANIPRGIALLLVAAVERSERLYAVMAVRGYSGSMAEKERARLTRHDW
                     LPLGTGAAVLLFVILWK"
     gene            complement(1027..1728)
                     /locus_tag="VFK23_06995"
     CDS             complement(1027..1728)
                     /locus_tag="VFK23_06995"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HET7318864.1"
                     /translation="MHIPDGVLPVWLWVLGFFLMSLIMGFSLYRLRSMDMKMKIPLLG
                     TLSAAMLVAMSLEIVPLAYHINLTVVTGILLGPALGFVAAFIANIMLALIGHGGITVI
                     GLNTLLLGAEAVLGHMFFSLLGNRLPLFWRAAVATVITLLCTTGLLIGIVAVSNANPA
                     AFMETHEHEAAGHEGPISTGRFAAMALGLGALGWIIEAAITGSVVRFIAKVKPDLLRH
                     VLHADTNSRKERQTP"
     gene            complement(1766..2167)
                     /locus_tag="VFK23_07000"
     CDS             complement(1766..2167)
                     /locus_tag="VFK23_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET7318865.1"
                     /translation="MLGFLLRWSINLLALIVAGSLIPGIRIQSMQMGIIAAGILGVVN
                     AVIRPVVLILTLPINLLTLGLFTLVINAALLKLVSDLVPGFTIESFGAAFLGALVISL
                     TSWVLNLFVNGDGTFIFIKRIHKDGDRGIRK"
     gene            2408..>3469
                     /locus_tag="VFK23_07005"
     CDS             2408..>3469
                     /locus_tag="VFK23_07005"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7318866.1"
                     /translation="MGISKRAQAVKPSPTLATAAKAKAMKAQGIDVVDFGVGEPDFDT
                     PDNVKQAGIQAIQNGFTKYTPAGGTDDLKEAVIEKFKRDNGLTYEKSQVLISCGAKHS
                     LYNIAEALFDPGDEVIIPAPYWVSYPDQVLLNDAVPVIVETTEAEGFKLSAKKLEKAI
                     TPKTKALVLNSPSNPTGLAYDRRTLEDIAALAVKRGIYVISDEIYEKLLYDGFTHTSI
                     ASLGPEIKNLTIVVNGVSKSHAMTGWRIGYAAGPKDVVSAMANIQSQSTSNPASISQK
                     AAVEALRGPQDFIGIMNAEFDKRRKYMIGRLNAMPGVSCLTPVGAFYAFPRVSSLYGK
                     SANGKKIGNSSDLAAYLLED"
BASE COUNT          800 a         1058 c         1033 g          578 t
ORIGIN      
        1 ccagacggtt ggagacagga tctgatcatc gacgttctgc aggagcacgc cgatgcgttt
       61 ccggatctcg ttgtaccgct gtgccgggtt gactccgaag acggagaccc tgccctcgtc
      121 cggcttgagc agccccagga tatggtagag gagcgtagtc ttgcccgatc cgttcccccc
      181 cagaatcgcg acacgctccc cgcggttcac cacgaagtcg agcccgcaga tatggatctc
      241 cgtggtgtcg gggtaggtgt gcttgaggca gcttacactg atgatcgttt ccatacgtcc
      301 ttcgtcttct ccgcctgact tctcacttcc agaggatcac aaacaggagg actgctgctc
      361 cggtaccgag cgggagccag tcatgccgcg tcagcctcgc ccgttccttc tccgccatgc
      421 tgccgctgta ccccctcacc gccatgacgg catagagccg ttcagaccgc tcgacggccg
      481 ccacaagaag cagggcaatg ccgcggggga tattggcaat cgtcttcacg atgcgccccc
      541 gggcaaggcc cgcgcggagc cggagcgcca cgataaagtt gttcatcatg tccagaagaa
      601 tgaaaaaagt ccggtaggtc atgaacaggc ccgcggccgg aagctccgga aggacggcgc
      661 tgatcaggga aaagagcctc ggatagggcg tgctcacgat gagcatgagc acggcgagcg
      721 caggggtcac cgctttgaga atcacaagac cataaacggc aaggccgacg ccgcgaaggc
      781 tgatgccgta gagcgccgcg aaaacggccg cgtagagcga caacatcgcg atccgcagca
      841 agggcagccg cgcggcggct gaggcggcaa gcaggagtgc gtatcccgcc gcaagggccg
      901 ggacgctgcg cgacagtacg gcggccgcga cgacaaacgc cacgaaaata agtttggcga
      961 gaacagacgc gcggtgaaaa gccccggaac cgctggccgc ccaatagtcg atccgcgcta
     1021 cgtcgatcac ggcgtctgcc gctccttcct tgagttcgta tccgcgtgca gcacatgccg
     1081 caggaggtca ggctttacct tggcgatgaa ccgcacgaca gagccggtga tcgcggcttc
     1141 gatgatccag cccagggcgc cgagacccag cgccatggcc gcgaaccttc ccgtggaaat
     1201 cgggccttcg tggccggccg cttcatgctc atgggtttcc atgaaggccg ccggattcgc
     1261 attcgagacg gcaacgatgc cgatgagaag gccagtggtg cacagaagcg ttatcacggt
     1321 ggcaaccgcg gcccgccaga acaggggcag acggttcccc aagagcgaga agaacatgtg
     1381 gccgagaacg gcttctgcgc cgagaagcag agtattcagg cctatgacgg tgattccgcc
     1441 gtgcccgatg agcgccagca tgatgttggc tatgaaggcg gcgacaaacc ccagcgccgg
     1501 acccagcaga atgccggtaa cgacggtaag gttgatatga tacgccaggg gcacgatctc
     1561 caggctcatc gccacgagca tggctgccga gagggtgccc agaagcggga tcttcatctt
     1621 catgtccatt gaccgaagac ggtacaggct gaatcccatg atcagggaca tgaggaaaaa
     1681 tccaaggacc cagagccaga cgggcaggac tccgtccggt atgtgcatat gcgtcatagc
     1741 gcgccgttcc ggacggtccc gcaggtcatt tccggatgcc tctatcgccg tccttgtgaa
     1801 tccgtttgat aaagataaag gtcccgtcgc cgttgacgaa gagattgagg acccaactgg
     1861 tcaggctgat aaccagcgcg ccaaggaacg ccgcgccgaa cgactcgatg gtgaagccgg
     1921 gaacgaggtc cgagacgagt ttcagcagcg cggcgttgat taccagcgtg aagagcccca
     1981 gcgtgaggag gttgatcgga agagtaagaa taaggacgac aggcctgatc accgcgttca
     2041 caacgccgag aatgccggct gcgatgatcc ccatttgcat gctttgtatc ctgattcccg
     2101 gtatcaggga ccctgctaca atcagcgcga gcaggttgat cgaccagcgc agcaaaaaac
     2161 cgagcatgaa acctcctcaa aaccaaaaaa atagtatcac acttcattcg actttgcaac
     2221 cggggagtgg cggaggtgaa agaggcgaag cgaaaaacgg accgcgtttt accgggtgcc
     2281 gccgggccct gtgccgtgag cttccggcgt tctctctcaa gaaactcgtt gacttcgccc
     2341 tgcttctatg ttaaaaaaat caggcttcat accgacacgt taaaagcgca gaaagggcac
     2401 gatcatcatg ggaatttcga aacgggcgca ggccgtcaaa ccctccccca cgctggctac
     2461 cgccgcgaag gcaaaggcca tgaaggcgca gggcatcgac gtcgtggact tcggcgtcgg
     2521 ggagccggac ttcgacacgc cggacaatgt caagcaggcg ggcatccagg cgattcaaaa
     2581 tggtttcaca aaatacaccc cggcgggcgg caccgacgat ctcaaagaag cggtcatcga
     2641 gaaattcaag agggacaacg gcctcaccta tgagaagtcc caggtcttga tctcctgcgg
     2701 ggccaagcac agcctttaca acatcgcgga ggccctgttc gatcccggcg atgaggtcat
     2761 catccccgcc ccctactggg tctcctaccc cgaccaggtc ctcctgaacg acgccgtgcc
     2821 cgtcatcgtc gagaccacgg aggcggaggg tttcaaactg tcggcgaaga agcttgaaaa
     2881 ggccatcacc ccgaagacca aggcgctggt cctcaacagt ccgtcaaacc ctaccggcct
     2941 cgcctacgac cggaggacgc tcgaggacat cgcagccctc gcggtcaaac gcggcatcta
     3001 cgtgatttcg gacgagatct atgaaaagct cctctacgac gggttcacgc acacgagcat
     3061 cgcatctctc ggcccggaga taaagaacct gacgatcgtg gtcaacggcg tttcaaagtc
     3121 ccatgccatg accggctggc gcatcggcta cgcggcaggc cccaaggacg tcgtctccgc
     3181 catggcgaac atccagagcc agagcacctc gaaccccgct tccatttccc agaaggcagc
     3241 ggtcgaagcg ctccgcgggc cccaggactt catcgggatc atgaacgccg aattcgacaa
     3301 gagaagaaaa tacatgatcg ggcgactgaa cgccatgccg ggggtgtcct gcctgacgcc
     3361 ggtcggggcg ttttacgcct tcccccgcgt ctcgtctctc tacggaaaga gcgcaaacgg
     3421 gaagaagatc ggaaactcct cggacctcgc cgcctatctg ctcgaggat
//
LOCUS       DASSLD010000385         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_620082,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000385 DASSLD010000000
VERSION     DASSLD010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620082"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /gene="rplJ"
                     /locus_tag="VFK23_07010"
     CDS             <1..456
                     /gene="rplJ"
                     /locus_tag="VFK23_07010"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET7318867.1"
                     /translation="AKTAVITGYTGINVEQITELRSKLRKSQVEYRVVKNTLARQAVQ
                     GTGLEPLKDYFVGPVGIALGFDDVVAPAKVLFEFNKTQEKLQLKIGVLDGKLLQQADI
                     KALATLPSLNALRGKIIGLLQAPASRMVGVLAAPGGQIARVLKAKAEKG"
     gene            524..901
                     /gene="rplL"
                     /locus_tag="VFK23_07015"
     CDS             524..901
                     /gene="rplL"
                     /locus_tag="VFK23_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET7318868.1"
                     /translation="MADLAKLVDELSGLTVLEAAELSKMLEEKWGVSAAAPVAVAAAG
                     GAAAGAAAVEEKTAFDVVLAAAGAQKINVIKVVRELTGLGLKEAKDLVEGAPKPVKTG
                     VSKEDAENMKKKLTEAGATVEVK"
     gene            1087..>2241
                     /locus_tag="VFK23_07020"
     CDS             1087..>2241
                     /locus_tag="VFK23_07020"
                     /inference="COORDINATES: protein motif:HMM:NF016450.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET7318869.1"
                     /translation="MSKEKRELVRVRKDFSRIPTILEIPDLIEIQKKSYDHFLQKDIA
                     SDKRKDMGLQAAFKSVYPIYDFNETAMVDYLGYSLGEPKYSIWECLQRGATFAAPLKI
                     KMRLVFFEKDEQTATKRIKDIKEQEVYVGDLPLMTETGTFIVNGTERVVVSQLHRSPG
                     AFFTHDKGRTHASGKILYSARIIPYRGSWLDFEFDVKDIIYVRIDRRRKIPATILLKA
                     FGYSNEDLLRMYYPIEDIRIRKEGFTRGIHPDVLAGLKAPVTITDQNTREQIVKEGAK
                     ITRVAIKKMEAAGITEIPISREELIDRIVLRDVIDPETGEVLLPANEKITEAFLTGVA
                     QTKVEKMEVIYMDGIHVIAAMRDTLLSDKVQTSDEALLEIYRKMRPGEPPT"
BASE COUNT          560 a          597 c          669 g          415 t
ORIGIN      
        1 gccaagactg cggtaattac cggctatacc ggaataaacg tcgagcaaat caccgagctc
       61 aggtcaaagc tgcgtaagtc ccaggtcgaa taccgggtcg taaaaaacac gcttgccaga
      121 caggcagtcc agggcactgg cctcgagcca ctcaaggact acttcgttgg gccggtaggc
      181 atcgcgctcg ggtttgatga tgtggtcgcg ccggcgaagg tgcttttcga gtttaacaaa
      241 actcaggaaa agctccagct caagatcggc gtgctcgacg ggaagctgct ccagcaggcc
      301 gacatcaagg cgctggcaac cctgccgtct ctcaatgcac tccgcggaaa gatcattggg
      361 ctgcttcagg cacctgcatc gcggatggtc ggtgttctgg cagcacccgg cggtcagatc
      421 gccagagtct tgaaggcgaa agcggaaaaa gggtaggctt acagagaagc attcggtgaa
      481 atttttcagt tgaattatag aatatttcca ggaggaaaag ataatggctg atttagcgaa
      541 actggttgat gaattgagcg gtcttaccgt tcttgaagcg gcagaattga gcaagatgtt
      601 ggaggagaag tggggcgtgt ccgcggcggc gcccgtagcc gtcgctgctg caggcggagc
      661 cgcagcagga gcggcggcgg tcgaagagaa gaccgcattc gatgtcgtcc ttgcggctgc
      721 aggggcgcag aagatcaacg tgatcaaggt cgtgcgcgaa ctcaccgggc tgggcctgaa
      781 ggaagcaaag gacctcgtgg agggcgcgcc caagccggta aagacgggtg tctccaaaga
      841 ggatgcagag aacatgaaga agaagctgac cgaggccggc gcaacggtcg aggtgaaata
      901 atcaagcatc gtcaaagcag gaacagccaa gtccttgccc tccggcaccg tccggagggc
      961 agaggacttt ttgcgtttat gacggcctgt gtcagcgggt caaaatgtca taggatcaca
     1021 acataaaacg ctgccacact tatgatactt tggtgctctg acacttgcgg ttaaaggggt
     1081 gtgattatgt caaaagaaaa aagagaactt gttcgggtca ggaaggattt ttccaggatc
     1141 ccgacgattc tggagatccc cgatctcatc gagatccaaa agaagtccta tgatcatttt
     1201 ctccagaagg atatcgcgtc cgacaaacga aaagacatgg ggcttcaggc ggcattcaag
     1261 agcgtctatc ctatctacga tttcaacgag accgccatgg tcgactatct cggctactcg
     1321 ctcggagaac cgaagtacag catctgggag tgcctgcagc gcggcgccac gttcgcggcg
     1381 ccgctcaaga tcaagatgcg tctcgtgttt ttcgaaaagg acgagcagac agccacgaag
     1441 cgcatcaagg acatcaagga gcaggaggtg tatgtcggag acctccccct catgaccgag
     1501 acgggcacgt tcatcgtgaa cgggacggag cgcgtcgtgg tgagccagct ccatcgctcg
     1561 ccgggggctt tctttaccca cgacaagggc aggacccatg caagcggcaa gatcctctac
     1621 tcggcccgga tcatccccta ccgcggctcc tggctggact tcgaattcga cgtgaaggac
     1681 atcatctacg tgcggatcga tcgccgcaga aagatacccg cgacgatcct cctcaaggcc
     1741 ttcggatatt cgaacgagga ccttctccgg atgtactatc cgatcgaaga tatccggatc
     1801 aggaaggaag gcttcacgcg gggcatccat cccgatgtcc tggccgggct caaggctccc
     1861 gtcacgatta ccgaccagaa cacgcgtgag caaatcgtga aagagggcgc caagatcacc
     1921 cgcgtggcga tcaagaagat ggaagccgcg ggcatcaccg aaattcctat ttcgcgcgag
     1981 gagctgatcg accgcatcgt gctccgcgac gtgatcgatc cggagaccgg agaggtgcta
     2041 cttccggcaa acgaaaagat caccgaggcg ttcctcacag gcgtcgccca gaccaaggtt
     2101 gagaagatgg aagtgatcta catggacggc atccacgtga tcgctgccat gcgcgatacg
     2161 ctcctgtccg acaaggtgca gacgtcggac gaagcactcc ttgagatcta ccggaagatg
     2221 cgtcccggag aaccgccgac c
//
LOCUS       DASSLD010000386         3782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_180187,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000386 DASSLD010000000
VERSION     DASSLD010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180187"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..626)
                     /gene="argB"
                     /locus_tag="VFK23_07025"
                     /pseudo
     CDS             complement(138..626)
                     /gene="argB"
                     /locus_tag="VFK23_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677082.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
     gene            complement(712..2055)
                     /gene="hslU"
                     /locus_tag="VFK23_07030"
     CDS             complement(712..2055)
                     /gene="hslU"
                     /locus_tag="VFK23_07030"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HET7318870.1"
                     /translation="MNSDLTPRQIVEELDKYIIGQHNAKRAVAIALRNRWRRQRLSEE
                     LRDEVMPKNIIMIGPTGVGKTEIARRLAMLVQAPFLKIEASKFTEVGYVGRDVDSIIR
                     DLTEIGINMVKSEMAEQVREKAEAMAEDRLLDVLLPPVRTAPGFDAPTTEQQEQHTTT
                     REKLRQQLREGKLDNRSVEIETKEKVMPFGIVSNVGMEEIEMNLKEMLGGLLPDKTKR
                     RKMKVPEARRLLVQEEASKLIDMDKVTREALKRVEQSGIVFLDEIDKIAGRETHGPDV
                     SREGVQRDLLPIVEGSTVTTKHGPVKTDHILFIAAGAFHVSKPSDLIPELQGRFPIRV
                     ELDSLGKGEFIRILTEPQNALIKQQVALLATEEIELGFSADAIEEIAGIAAQVNERTE
                     NIGARRLHTIMERLLDEISFDAPDKKGAKININAGYVRERMADILKSEDLSRYIL"
     gene            complement(2181..2723)
                     /gene="hslV"
                     /locus_tag="VFK23_07035"
     CDS             complement(2181..2723)
                     /gene="hslV"
                     /locus_tag="VFK23_07035"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HET7318871.1"
                     /translation="MSEIPPLHGTTILAVRRNGKTAIAGDGQVTVGATVMKHNAKKVR
                     KMYNDKILAGFAGATADAFTLFEKFESKLEQYHGNLTRAAVELAKDWRTDKVLRRLEA
                     LMIVADNERSFILSGNGDVIEPENGIAAIGSGGPYALAAARALTDHTSLDARSIVTKA
                     LEIAADICIYTNREITIEEL"
     gene            complement(2814..3659)
                     /gene="xerA"
                     /locus_tag="VFK23_07040"
     CDS             complement(2814..3659)
                     /gene="xerA"
                     /locus_tag="VFK23_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HET7318872.1"
                     /translation="MSPNTERNYLSDLEQFADFLGEVEPAEVDHRRIRDFMGHLMKQK
                     EKKSSIARKLSAIRSFFRYLNKKGFITGNPARLVATPRREKRLPAVLTADDAQRLLDT
                     PGKQNNDRTELRDRAVLETLYSTGIRAAELIGMNRDDINREDRLIRIRGKGRKERIVP
                     IGRTALGSLDAYLAHKKEGAENPAVFTGPTGNRLTVRTVQRILENSRKKLGLAQKASP
                     HTLRHSFATHLLEAGADLRAIQELLGHASLSTTQRYTHVNLDSLMEAYDKAHPRAKSR
                     KDEGR"
     gene            3570..>3782
                     /locus_tag="VFK23_07045"
     CDS             3570..>3782
                     /locus_tag="VFK23_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318873.1"
                     /translation="MIYFGRFDFAEEIRELLQVGKIIPLGVGRHVTFIPEIGHEIEDM
                     LSHATTLAYRKGFCKAERAGQPWRTQG"
BASE COUNT          665 a         1091 c         1125 g          901 t
ORIGIN      
        1 gcggcaaagc ggtaggtctc acgggcaaag acggcgggct gatccatgca aaaaagctca
       61 agatgactaa aaaatcagag gacaccggag aaacggagat catcgacatc ggcctcgtcg
      121 gcgaggtcac cgaaatccgg ccggacttcg gtgacctcgc cgacaagtcc gatgtcgatg
      181 atctccgtct ctccggtgtc cgccgattgt ttcgtcatct tgagcttctt tgcatgaatc
      241 aaaccgccgt ccttgcccgt aagacccacc gctttgccgc ccgcccggtt gatgagcgtc
      301 acgatctcct tgttgacctt gccgcccagg accatttcaa cgatgtccat ggtctcctgg
      361 tcggtcaccc gctggcccga aacaaaagtc ggtttcttgc ccatctgctc catggtcttc
      421 ccgatctgcg ggccgccgcc gtggacgatc acgggattga tgccgatgta ccggagaaga
      481 acgacgtcct gggcgaaacc gtgcttgagc gtttcgtcga tcatggcgtt tccgccgtat
      541 ttgatgacga aggtcttgcc ccggaaggtc tggatgaaag gcatggcctc gataagagtt
      601 ttggcttttt cgatcagctt ctgcatgaaa atcccggttt cgagttgcgt gttgcgagtt
      661 gtgaagtacg ggttaaacaa gcaaccataa cgcgtaaccc gcaacgtggt gttacaggat
      721 gtacctgctc aaatcctcgc tcttcaggat gtcggccatg cgctcgcgca cgtacccggc
      781 gttgatgttg atctttgcgc cttttttatc gggagcgtcg aaggagatct cgtcgagcag
      841 ccgctccatg atcgtatgca gcctgcgcgc cccgatgttc tccgtccgct cattcacctg
      901 cgcggcgatg ccggcgattt cctcgatggc atcggcggaa aaaccgagtt cgatttcttc
      961 cgtggcgagc agcgccacct gctgcttgat gagggcgttc tgcggctccg tcaaaatgcg
     1021 gatgaactct cctttgccca gggaatcgag ttcgacccgt atcgggaagc ggccctgcag
     1081 ttccggtatg aggtcggacg gcttcgacac gtgaaacgcg ccggcggcga tgaacagaat
     1141 atggtcggtt ttcaccgggc catgcttggt cgttaccgtc gaaccttcga cgatggggag
     1201 gaggtcccgc tgcacgccct cgcgggacac gtccgggcca tgtgtttcgc ggccggcgat
     1261 cttgtcgatt tcatcgagaa acacgatgcc cgactgctcg acacgcttca aggcctcgcg
     1321 cgtaaccttg tccatgtcga tgagctttga cgcctcctcc tgaaccagca ggcggcgcgc
     1381 ctcgggtact ttcatcttgc gccgctttgt cttgtcgggc agcagtccgc cgagcatctc
     1441 cttgaggttc atctcgatct cttccatgcc gacgttcgag acgatgccga agggcatgac
     1501 cttttccttc gtctcgatct caacggaccg gttgtcgagc ttcccttcgc gcagctgctg
     1561 gcgcagcttt tcgcgcgtgg tggtatgttg ctcctgctgc tccgtggtcg gggcgtcgaa
     1621 gcccggcgcg gtcctgaccg gcggcaggag gacgtcgagc aggcggtcct cggccatggc
     1681 ctcggccttc tcccggacct gttccgccat ttcacttttt accatgttga tgccgatttc
     1741 ggtgagatcg cggattatgg agtcgacgtc ccggcccacg tagcccactt cggtgaactt
     1801 ggacgcctcg atcttgagga acggcgcctg aacaagcatc gcgagtctcc gggcgatctc
     1861 ggtcttgccg accccggtag gaccgatcat gatgatgttc ttcggcatga cctcgtcacg
     1921 gagctcttcg ctcaggcgct ggcgacgcca gcggttcctg agagcgatgg caacggcccg
     1981 tttggcgttg tgttgaccga tgatgtattt gtcgagctcc tcgacgatct gccggggagt
     2041 aagatcgctg ttcaagaaaa cctccgcgga aatggttact agactctaga ctgtagaaag
     2101 cagattgtag gctgtaaact gcgttcggct gtttcgtctc tcttctctgt ctcctgtcta
     2161 ctgtctccag tgtactatct tcacagttct tctatcgtta tttccctgtt cgtgtagatg
     2221 cagatgtctg ccgcaatctc caaggccttt gtcacgatgg aacgggcgtc cagcgacgtg
     2281 tggtccgtga gcgcccttgc tgccgcaagg gcataggggc cgcccgagcc gatcgccgca
     2341 atgccgttct ccggctcgat gacgtcgccg ttgcccgaca ggataaagga gcgttcgttg
     2401 tcggcgacga tcatgagcgc ttcgagccgg cgcaacacct tgtccgtgcg ccagtctttc
     2461 gcaagttcga cggccgcgcg ggtaagattg ccgtgatact gctccagctt ggattcgaat
     2521 ttttcgaaca gcgtaaaggc gtcggcggtt gcgccggcga aacccgcaag gatcttatca
     2581 ttgtacatct tgcgcacttt cttagcgttg tgcttcatca ccgtggcgcc gacggtgacc
     2641 tgcccgtcgc cggcgatggc ggttttcccg tttcgccgca cggccaggat agtagtcccg
     2701 tgcagggggg ggatctcact catgatgcct cctgtttaat gccgatggac gacagacgat
     2761 cgttcgctcc gctcacgagg acgatggacg agttaagacc ttgcgtccat cgtctatcgt
     2821 ccctcgtcct tccgactctt cgctctcgga tgcgccttgt cgtaggcctc catgagcgaa
     2881 tcgaggttta cgtgcgtata acgctgggtt gtcgagaggc tcgcgtgtcc gagaagctcc
     2941 tggatggccc gcaggtcggc gcctgcctcg agaaggtggg tcgcaaaact gtgccggagc
     3001 gtgtgcggac tcgccttctg cgccaggccg agttttttcc tggagttttc taaaatacgc
     3061 tgaaccgtcc ggactgtcaa gcgatttccc gtgggtcccg taaaaacggc ggggttttcc
     3121 gcgccttctt ttttatgggc gaggtaggcg tcaagggagc cgagcgcggt ccgtccaatg
     3181 ggcacgatcc gctctttccg tccctttccg cgaatgcgaa tgagccggtc ctcgcggttg
     3241 atgtcgtcgc ggttcatgcc gatgagttcg gcggcgcgga tgccggttga atagagcgtc
     3301 tcgagcacgg cgcggtcccg gagctctgtc ctgtcgttgt tttgtttacc cggcgtatcc
     3361 agaagacgct gggcgtcgtc ggctgtcagc accgcgggca gacgtttttc gcggcgcggc
     3421 gtggcgacga ggcgcgcggg attgccggtg atgaagccct ttttgttcag atacctgaag
     3481 aacgagcgga tggccgagag tttccgcgcg atggaggatt ttttctcctt ctgtttcatg
     3541 agatggccca tgaagtcccg gatcctccga tgatctactt cggccggttc gacttcgccg
     3601 aggaaatccg cgaactgctc caggtcggaa agataattcc gctcggtgtt gggagacacg
     3661 ttacgttcat tcctgagata ggccacgaaa tcgaagatat gctttcgcat gcaactactt
     3721 tagcgtaccg gaagggattt tgcaaggcag aaagggcagg acaaccatgg aggacacagg
     3781 ga
//
LOCUS       DASSLD010000387         3974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1227889, whole genome shotgun sequence.
ACCESSION   DASSLD010000387 DASSLD010000000
VERSION     DASSLD010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227889"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1555)
                     /locus_tag="VFK23_07050"
     CDS             complement(<1..1555)
                     /locus_tag="VFK23_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET7318874.1"
                     /translation="MKLVIDGKAVTVNRGATILEAAQALGVHIPTLCHDDRLHPYGAC
                     RICMVEVEGPPRRMLPACTTPAAEGMSVLTRTPLLAEARRSVLEFLLIHHPLECPVCD
                     KAGECRLQDLVHEYGLGPGLFAEEKRNPPPDYASALIDRNPNRCILCGKCVRSCKERC
                     GVGALTFSQRGGKSRVSTAFDGPHDCEFCGECIEICPVGALTAKQSKFNVRPWNLEKT
                     PSTCNYCGCGCPITVETRGNAVVRVRSAGDRYLCAKGRFGWDALLHESRLAAPLIRAG
                     GRLVDSTWDEALSVVATNLKVIGSRFGADRIGGLGSTRTTNEDDYVFQKFMRTVVGTN
                     NVDLLARAKYPYGLNAAYFAADLERLAEHDVILMLDGDAGEINALTGVEIVKAVNRRG
                     SRLILAAGGRSKFNRIASVALPQEGALDFLIGGLRNSRRIVSARIKQATDLLSSAKST
                     AIVLPARLTPDAFALIAELADILKHVDFYPVVTRGNIQGALDMGVLPDYLPGYQPAAG
                     RTAAMFGQEW"
     gene            complement(1608..2918)
                     /gene="nuoF"
                     /locus_tag="VFK23_07055"
     CDS             complement(1608..2918)
                     /gene="nuoF"
                     /locus_tag="VFK23_07055"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HET7318875.1"
                     /translation="MEQMLLKRSGIPRAADIDVAMAHGAYASVATAYGMRPVDVIDEV
                     KRSGLRGRGGAGFPTGVKWGFAAMDPKTPKYLIANADEGEPGTFKDRFILEGDPHLLI
                     EGMIIAGYAIGAERGYIYLRGEYPRAADILNRAIAQAYAKGLLGNNLAGKGLRFDLGL
                     HRGAGAYICGEETSLIESLEGKRGQPRLRPPFPANAGAWGMPTVVNNVETLANVPYVI
                     ALGSDVYAKIGAEGSPGTKIFSVSGSVNRPGGYELPLGTKLREIIDTWAGGVKDGRRL
                     KAVIPGGASTPMLTSEHLDVGMDFNSLLSAGSMLGSGAVIVMDETVCMVHAAAILVRF
                     FAHESCGKCTPCREGTGWLHQVYSRMLSGGGRKGDVELLESVCAKMKGKCFCPLGEGS
                     INPVLSSIRYFRNDYEQCIRNQGCGRQKCRDRQIGSKQVVQHSL"
     gene            complement(2924..3388)
                     /gene="nuoE"
                     /locus_tag="VFK23_07060"
     CDS             complement(2924..3388)
                     /gene="nuoE"
                     /locus_tag="VFK23_07060"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HET7318876.1"
                     /translation="MLSDDVRERIRLAQLRYPDRRGALLPALHIVQAELGRLSDEAMQ
                     FVARELDVPEPDVYGTATFYSLLRRAPAGRHVIAICHNLPCTLLGAEPLIEHLEKKLG
                     VVEGEVTPDGRFSYQRIECIGRCDGAPAMLVDGDYHGELTPEKIDAVLERYL"
     gene            complement(3391..3870)
                     /locus_tag="VFK23_07065"
     CDS             complement(3391..3870)
                     /locus_tag="VFK23_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7318877.1"
                     /translation="MVDPKDISRISLFEGLNDAERISIANVMTGDTFSEGHVVYRENE
                     VGCSCVYIIKRGKVDVLKMSTDGDPLTLAVLKEGNFFGEFSFFDHKPHSASTVVSAPD
                     TVVLSLQRPDFDQVAEKYPMIGYKVLINIVQEISAVIRRMNASYVDMAGYMFGRARR"
BASE COUNT          706 a         1276 c         1222 g          770 t
ORIGIN      
        1 tccattcctg cccgaacatc gccgcggtcc gcccggcagc aggctggtag ccgggaagat
       61 aatccggcag aacgcccatg tccagcgctc cctggatgtt gccccgcgtg acaacgggat
      121 aaaagtcgac gtgcttcagg atgtccgcga gttccgcgat gagcgcgaag gcgtcgggag
      181 taagccgcgc cggcaggacg atggccgtgc tcttcgccga cgagaggagg tccgtggcct
      241 gtttgatccg cgcactcacg atcctcctgc tgtttctcaa gccgccaatc aggaagtcga
      301 gcgccccctc ctgcggaagg gcgaccgacg cgatcctgtt gaatttgctc cgtcctcctg
      361 ccgcgaggat gagacggctg ccccggcggt ttacggcctt gacgatttcg acgccggtca
      421 gggcgttgat ttcgcccgcg tccccgtcca gcatgaggat gacgtcatgc tctgccagcc
      481 gctcgagatc cgccgcgaag taggcggcat tcagaccgta aggatacttg gcgcgggcca
      541 ggaggtccac gttgttcgtt cccaccacgg tccgcatgaa tttctggaag acgtaatcgt
      601 cctcgttcgt ggtcctcgtg gagccgaggc cgccgatgcg gtcggcgcca aagcggctgc
      661 cgatgacctt gagattggtc gcgacgaccg aaagggcctc gtcccaggtg ctgtcgacca
      721 ggcgtcctcc cgcccgtatc agaggcgcgg cgagacgcga ttcatgcaga agggcgtccc
      781 agccgaagcg gcccttggcg cagaggtagc ggtcgccggc ggagcgcacc cgcacgacgg
      841 cgttcccgcg tgtctcgacg gtgatggggc agccgcagcc gcagtagttg caggtcgagg
      901 gggtcttttc gaggttccac ggccgtacgt tgaatttgga ctgcttcgcc gtgagcgcgc
      961 cgaccgggca gatctcgatg cattctccgc agaactcgca gtcatgcggg ccgtcgaagg
     1021 cggtgctgac gcgggatttt ccgccccgct gactgaaggt gagcgccccc accccgcacc
     1081 gctccttgca ggacctcacg catttgccgc agaggatgca gcggttcggg ttccggtcga
     1141 tgagggcgga tgcgtaatcg ggaggcggat tcctcttttc ctcggcaaag agcccgggcc
     1201 ccagcccgta ctcgtgcacg aggtcctgga ggcggcactc gcccgctttg tcgcataccg
     1261 ggcattccag ggggtgatgg atgaggagga attcgaggac cgacctgcgc gcctctgcga
     1321 ggagcggcgt ccgggtcagg acggacatgc cctccgccgc cggcgtcgtg cacgcgggaa
     1381 gcatccgcct gggcggaccc tccacttcga ccatgcagat cctgcacgcc ccgtagggat
     1441 gaagccggtc gtcgtggcag agggtcggga tgtgcacccc caaggcctga gccgcttcga
     1501 ggatcgtggc gccccggttc acggtcactg ccttcccgtc gatgacgagt ttcataggga
     1561 acatccgttt cgggtctgga gttaccggtc gagcagcgaa ctccagacta cagactgtgc
     1621 tgtactacct gtttgcttcc aatctgccgg tcccggcact tctgtcggcc gcacccctga
     1681 ttcctgatgc actgttcgta atcattccgg aaatatctga tgctcgaaag cacggggttg
     1741 atcgatccct ctcccagcgg gcagaagcac tttcccttca tcttggcgca gacggactcc
     1801 agcagttcga catctccctt tcgtccgccc ccggaaagca tccgggaata gacctggtgg
     1861 agccagcccg tgccttcccg gcagggcgtg cacttgccac aggattcatg ggcaaagaag
     1921 cggacgagga ttgccgcggc gtggaccatg cagaccgtct cgtccatcac gatgaccgct
     1981 ccggagccga gcatggaacc cgccgacagg agggaattga agtccatgcc gacgtccagg
     2041 tgctcgctcg tgagcatggg cgtcgaggcg ccgccgggga tgactgcttt cagccgcctg
     2101 ccgtctttca cgcctcccgc ccaggtatcg atgatttccc ggagcttcgt tccgagaggg
     2161 agctcgtatc cgcccggccg gttgaccgag ccgctgaccg agaagatctt cgtgcccggc
     2221 gagccctccg ccccgatctt cgcgtagaca tcggaaccca gggcgattac atacgggaca
     2281 ttggcgaggg tctccacgtt gtttaccacg gtaggcatgc cccacgcgcc cgcgttcgcg
     2341 ggaaagggcg gcctgagcct cggctggccc cgcttgcctt caagcgattc gatgagggac
     2401 gtctcctcgc cgcagatgta ggcgcctgcg ccgcgatgca ggcccaggtc gaaccgcaag
     2461 cccttgcccg cgagattgtt tccgagtagc ccctttgcat acgcctgggc gatcgcgcga
     2521 ttgaggatgt ccgcggcacg cggatactcc ccgcgcaggt agatgtatcc ccgctccgcc
     2581 ccgatggcgt atcccgcgat gatcatgccc tcgatcagca ggtgcgggtc tccctcgagg
     2641 atgaatcggt ccttgaatgt cccaggctcg ccttcgtcgg cgttcgcgat aagatacttg
     2701 ggcgtcttgg ggtccatggc cgcaaagccc cacttcacac ccgtcgggaa gccggcccct
     2761 ccccggccgc gcaggcccga gcgtttcacc tcatcgatga catccacggg ccgcatcccg
     2821 tacgcagtcg ccacggaagc ataggcgccg tgcgccatgg ccacgtctat gtccgcggcc
     2881 ctcggaatgc ccgatctttt cagaagcatt tgttccattg cggttacaaa taccgttcca
     2941 gtacagcgtc gatcttctcg ggcgtcagtt caccgtgata atcgccgtct accagcatcg
     3001 ccggcgcgcc gtcgcacctg ccgatgcact cgatccgctg gtatgagaac ctgccgtccg
     3061 gagtgacctc tccttcgacg acaccgagtt ttttttcgag gtgctcgatg agcggctcgg
     3121 cgcccaggag agtacagggc aggttgtggc agatggcgat gacgtgcctg cccgccggag
     3181 cgcggcgaag gagcgagtag aacgtggccg tcccgtagac gtccggctcc gggacgtcca
     3241 attcccgggc aacaaattgc atggcctcat cggacagacg tcccagctct gcctgcacga
     3301 tgtgcagtgc cggcaagagc gcccccctgc ggtccgggta ccggagctgt gcaagacgaa
     3361 tccgctccct gacgtcatcg ctcagcatgc tcacctccgc gccctgccga acatgtagcc
     3421 ggccatgtcc acgtagctcg cattcatccg cctgatgacg gcgctgatct cctggacgat
     3481 gttgatgagg accttatacc cgatcatggg atatttctcc gccacctggt cgaagtccgg
     3541 ccgctggagc gacagcacaa cggtatcggg ggccgaaaca acggtgctgg ccgaatgggg
     3601 cttgtggtcg aaaaaggaga attccccgaa gaaattgcct tccttcagca ccgcaagggt
     3661 gagagggtcg ccatcggtgc tcatcttcaa cacgtccacc ttgccgcgct tgatgatgta
     3721 gacgcacgag catccgacct cgttttcgcg gtagaccacg tggccttcgg aaaaagtatc
     3781 gccggtcatg acgtttgcga tgcttatgcg ttcggcatca ttcagccctt caaacaggga
     3841 tatcctcgat atgtcttttg gatcgaccat ggaagcctcc gcgaggttaa aaatcggcgg
     3901 gaagtgcaga gtctcttctc gcgtccgtca taatgccaac ctgaaaattt attcaggaaa
     3961 aaagatgcaa tatt
//
LOCUS       DASSLD010000388         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1485853, whole genome shotgun sequence.
ACCESSION   DASSLD010000388 DASSLD010000000
VERSION     DASSLD010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485853"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VFK23_07070"
     CDS             complement(<1..893)
                     /locus_tag="VFK23_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7318878.1"
                     /translation="MEALEKTELSILSEISRISNSAIGLTEKLRRIVEVVARGMGRDG
                     ASVFLIDRSGKTVTLAAAVGLNQEPIGRMSFSLGMGIAGWVAEQKVPLALEDPYSDPR
                     FQYVPESGIGKFKSLVAAPIMDEERCLGVIFVLSVSSWRATSADLVLLTTTANQISGV
                     IKSTQLIQSIQDRLSELATIYEVGMALTSTLDLEQLLSLIAKNSAQSLRAQGCTIRLL
                     NLPEIAARKTFASYTVTGDAISSVDARIDHAVTDRVAQDKRPLLVQDIRSDPTLACPA
                     DSACSVISVPLLYRDKLIGIIT"
     gene            complement(982..>2426)
                     /gene="glgA"
                     /locus_tag="VFK23_07075"
     CDS             complement(982..>2426)
                     /gene="glgA"
                     /locus_tag="VFK23_07075"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET7318879.1"
                     /translation="KILFASSEAAPFAKTGGLADVAGSLPPALAELGHDVIVVMPRYR
                     QVDVETYKLKPVASLSIPLGTWQERCDVLRGRMGRNVTVYFIRKDSYYDRPGLYGAGG
                     TDYPDNAERFIFFSRAIPELCVALNLRPDIVHCNDWQTGLVPLYLKKMYHAHASLSRA
                     KSVFTVHNLGYQGLFWHWDMKLTGLGWDVFTPEGLEFWGRMNLLKAGLVYADSITTVS
                     RTYSREIQTPEFGYGLEGVLVRRAADLYGILNGIDYAEWNPVRGRSLPRSCSPAGLAG
                     KAVCRKKILKSLGLPDAGLPLIGMVTRLVDQKGLDIVTEALPGILSLGVQFVILGTGE
                     EAYERLLADAAIRHHDRMRVLLQYNEETAKNIYAGCDLFLMPSQYEPCGLGQMIALRY
                     GAVPVVRKTGGLADTVVDYDARTGRGTGFVFEEYSSQALLACVRRALRVFDDRKRWKR
                     LLEAGMKQDFSWKNSAKEYVKVYRETMRKK"
BASE COUNT          465 a          738 c          736 g          487 t
ORIGIN      
        1 gttatgatcc cgatcaactt gtcgcggtac aacagcggaa cgctgatgac ggagcaggcg
       61 ctgtcagccg ggcacgcgag cgtggggtcg ctgcgaatgt cctggaccag aagcggccgc
      121 ttgtcctgcg ccacccggtc cgtaacggca tgatcaatcc tcgcgtcaac actcgagatg
      181 gcgtctccgg tgacggtgta ggacgcgaag gtcttgcggg ctgcgatctc cggcaggttc
      241 aggagccgga tcgtacagcc ctgagctcga agggactgtg cactgttctt ggcgatgagc
      301 gaaaggagct gctccaggtc gagcgtggag gtaagcgcca tgcccacctc gtaaatcgtt
      361 gcgagctccg acaggcggtc ctggatgctc tggatgagct gggtgctttt aataacgccg
      421 ctgatctggt ttgccgtcgt cgtgagcagg accaggtccg cggaggtggc ccgccaggag
      481 gagacggaga gcacgaaaat cactcccagg cagcgttctt cgtccatgat gggtgcagcc
      541 acaagggact tgaattttcc gatccccgat tccgggacat actggaaccg cgggtcggaa
      601 tacgggtcct cgagtgcgag cggcaccttc tgctcagcca cccatcccgc gatgcccatc
      661 ccgagcgaaa agctcatcct gccgatcggc tcctggttca gcccgacagc ggcggcgagg
      721 gtgacggttt ttcccgaacg gtctatgagg aacaccgacg caccgtccct gcccataccg
      781 cgcgcgacga cttcgacgat gcggcggagc ttctccgtga ggccgattgc ggagttcgag
      841 atacggctga tttcgctcag gatgctgagt tccgtttttt cgagcgcttc catggataaa
      901 ccttaggggc cggtagccag ggtccggggt ccgggaaaag ccaaatgtgt cccctggtcc
      961 ttggctacag gctactggat cctatttttt cctcatcgtc tcccggtata ccttcacata
     1021 ttcttttgcc gagtttttcc aggaaaagtc ctgcttcatc ccggcctcca ggaggcgctt
     1081 ccatcttttc cggtcatcga agaccctgag cgcccgccgc acacaggcaa gaagcgcctg
     1141 ggaagaatat tcttcgaaga cgaagccggt tccccggccc gtccgtgcat cgtagtcaac
     1201 gaccgtatcg gcgagtccgc cggttttcct gacaaccggc acggcaccgt accgaagcgc
     1261 tatcatctgg ccgagtccgc acggttcata ctgcgacggc atgaggaaca ggtcgcatcc
     1321 cgcatagata tttttggcgg tttcctcgtt gtactgcagg agcacgcgca tccggtcatg
     1381 gtggcggatt gccgcatcgg caagcaggcg ttcgtatgct tcttcgccgg tccccaggat
     1441 gacgaactga acgcccaggg acagaatgcc cggcagtgct tctgtgacaa tatcgagccc
     1501 cttctggtcc acgaggcgcg tgaccatgcc gatcaggggg aggccggcat ccggcagccc
     1561 gaggctcttg aggatctttt ttctgcacac cgcttttccg gcaaggcccg cgggactaca
     1621 ggaacgcggc agggagcggc cgcggacggg gttccactcc gcgtagtcga tcccgttcag
     1681 gatgccgtaa aggtccgcgg cccgtctgac gagcactccc tcgagaccat agccgaactc
     1741 gggcgtttgg atctcgcggc tgtaagtccg gcttacggtt gtaatgctgt cggcgtacac
     1801 cagtccggcc ttgaggaggt tcatcctgcc ccagaattca agtccctcgg gcgtgaacac
     1861 atcccagccc aggccggtga gtttcatgtc ccaatgccag aacagcccct ggtagccgag
     1921 gttgtgcacc gtgaacaccg actttgcccg gctgagcgac gcatgggcat ggtacatctt
     1981 tttcaggtag agcggcacca gccccgtctg ccagtcgttg cagtgcacga tatcgggccg
     2041 taggttcagg gcaacgcaga gttccggaat cgcacgcgaa aaaaaaataa agcgctcggc
     2101 attgtcggga taatccgtcc cgccggctcc gtaaagtccc ggccggtcat agtaactgtc
     2161 tttccggatg aaatagaccg tgacgttcct gcccatcctg ccgcgaagca cgtcgcaccg
     2221 ctcctgccac gttcccaggg ggatggacaa tgaggcaacc ggcttgagct tgtatgtctc
     2281 aacatccacc tgacggtagc gcggcatgac gacgatcacg tcgtggccca actccgcgag
     2341 ggccggtgga aggctccctg caacgtctgc cagtccgccg gtcttggcga agggcgccgc
     2401 ctcagaggag gcaaagagga ttttca
//
LOCUS       DASSLD010000389         2525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_327029,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000389 DASSLD010000000
VERSION     DASSLD010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327029"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..136
                     /locus_tag="VFK23_07080"
     CDS             <1..136
                     /locus_tag="VFK23_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HET7318880.1"
                     /translation="GGDVFVHFGDIQDSGFKSLAEGQSVQFDVVDSPKGPKATNVVKL
                     "
     gene            complement(215..823)
                     /locus_tag="VFK23_07085"
     CDS             complement(215..823)
                     /locus_tag="VFK23_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318881.1"
                     /translation="MNNIPNIAPLVEEALKDHGKLRNIVSLLYHEDMAERLRAALALG
                     EITRRDPELMLQRWQRIFRAFDDTMSCWGVAEALAEIARNMPKEHRGKLVNFLRGFKR
                     DDCSCQGYIWGMCRICKIEGGRVNDFVPELEAFLDSQNICMRAQALWALGELARKESA
                     ERIRAFLKDVNEVWIFEDGSVSRKSIGTIAGEALKKMSVDQT"
     gene            complement(1151..1357)
                     /locus_tag="VFK23_07090"
     CDS             complement(1151..1357)
                     /locus_tag="VFK23_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318882.1"
                     /translation="MKKIIALCTAMAFALTLGVAFAEETTATPPAPTAEKAPAKHKKH
                     DKQKKHRKAKKESTAPEATAPAAK"
     gene            complement(1617..2195)
                     /locus_tag="VFK23_07095"
     CDS             complement(1617..2195)
                     /locus_tag="VFK23_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HET7318883.1"
                     /translation="MNVFNILISGVGGQGVLLTSKIIAEAALLSGLDVKQSEVHGMAQ
                     RGGSVLSQVRFGEKVFSPIVSEGEADLLIGFEPLETARYLHFLKDDGAVIYNTRQIGT
                     IGVSIAVEKYPADVNDIIKKRVKTVMPFDGTRLAVSAGDKRTLNLVLLGAALKFLPLK
                     EQAVLDAVVNTVPKKVLAINQKAFAAGRAQGK"
BASE COUNT          498 a          694 c          708 g          625 t
ORIGIN      
        1 aggcggagat gtgttcgttc actttggaga cattcaggac agcggcttca agtcgcttgc
       61 tgaaggccag tctgtgcaat tcgacgttgt cgattcgccc aaaggcccca aggcgaccaa
      121 cgttgtaaaa ctctaagcca catcacaccc atcgaagcag gcccgggcaa tccggtgcct
      181 gctttttttt cgtgagggat tgtggaaggt tccgctatgt ctgatcaacg ctcatttttt
      241 tcaaagcctc tccggctatc gttccgatac ttttcctgga cactgaaccg tcttcgaaga
      301 tccacacctc atttacatct ttaaggaatg ccctgatcct ctccgccgat tcctttcttg
      361 cgagctctcc cagagcccag agcgcctggg ccctcatgca gatattctga gagtccagga
      421 atgcctcaag ttcaggcacg aagtcgttta cccgtccgcc ttcgatcttg catatcctgc
      481 acattcccca tatatagccc tggcagctgc aatcgtccct cttgaatccc cggaggaagt
      541 tgacgagctt tccccggtgc tctttcggca tgttccgggc gatttccgcc agcgcctccg
      601 ccacacccca gcagctcatg gtgtcgtcga acgcccggaa gatacgctgc caccgttgca
      661 gcataagttc cggatcgcgc ctggtgatct cgcccagggc cagggcggcg cgaagccgct
      721 ccgccatgtc ctcgtgataa aggagggaca cgatgttccg gagtttcccg tgatccttga
      781 gcgcctcctc gacgaggggg gcgatgttcg ggatgttgtt catttctgct cttctaaatt
      841 gctctgcgtg aattgtgaca aagagctgtt tttatcagta taatcgcgtc gtgtctctgc
      901 aaaaaaggat acaataacga cggggaaatg gcaaggggat tcaacgagcg ggcggtaagt
      961 gacgggtctg agagcacgtc tattttactt taaggcgaag ggcggcccat gcgggatgga
     1021 atgccggctc gtttacttcc cctgcggcct gcatgaaaaa aggcccgcca gttcgactgg
     1081 cgggcctttt cctgcacatg atagataact gtttccgctc tgtattatga attagcggta
     1141 tgggggcgga ctattttgcc gcaggtgctg ttgcttccgg agctgttgat tccttcttgg
     1201 ccttcctgtg cttcttctgc ttgtcgtgtt tcttgtgctt tgcaggggcc ttctccgcag
     1261 tcggggccgg aggagtcgct gtcgtctcct ctgcgaacgc tacgccgagg gtcagagcaa
     1321 acgccattgc cgtgcaaaga gcgattatct tcttcatagg tattcacctc ccttcttgtt
     1381 ggaattggcc ggatgctgct tgctgatgta tccgatatga ataatgagac acgggcgatt
     1441 ggaaaaggtt taatgccgtc aaggcctcca gagagagcga aagacaaagt tttttgcggc
     1501 ttctgctgca tcacgtttta ttcacggatg aacgtggcaa aatgctcgct gttttattaa
     1561 agcctcggat tatggcagct gcgtgaatgc tgcagaggga tggaatgccg gttcgtttac
     1621 tttccctgcg ccctgcctgc ggcaaaggcc ttctggttga tcgcgaggac cttcttgggg
     1681 actgtgttca caacggcgtc gaggacggcc tgttctttca gagggagaaa cttgagcgcg
     1741 gcgcccagga gcacgaggtt gagggtcctc ttgtcgccgg cggaaacggc aagccgggtg
     1801 ccgtcaaagg gcatgacggt ctttacgcgt ttttttatga tgtcgttcac gtctgcggga
     1861 tacttttcca cggcgatgga gacgccgatt gtgccgatct ggcgagtgtt gtagatgacc
     1921 gcgccgtcgt ccttcaaaaa atggaggtag cgggcggttt ccaaaggctc gaacccgatc
     1981 aggaggtcgg cttcgccctc ggagacgatc ggcgagaaga ccttttcccc gaagcgcacc
     2041 tgcgagagca cgctcccgcc gcgctgggcc atgccgtgca cctcgctctg cttcacgtcg
     2101 aggccggaaa ggagcgccgc ctcggcgatg atcttgctcg tgaggagcac tccctgcccg
     2161 cccacgccgg atatcaatat attgaatacg ttcacgataa acctcgaaaa actttttgga
     2221 cgcagatttt cgcagatgat cgcggattaa aaaacctgga ttcatccccg aatgaaccta
     2281 tcggggatag aagcattcgg gaatgacggc tttgttgatg ccgcattccc aattccgcat
     2341 tccgcatttg tgttacgtcg tcttcatgca ccccgtggcg cagagctggt cgcagacgcc
     2401 gcagccgttg cacaggagcg gatcgatctc gacgaatccc gtctttccct cgggcgtata
     2461 ggagagcgcg atgcagccgg ccttgaggca gagcttgcag cccttgcagg attcccggtc
     2521 gaccg
//
LOCUS       DASSLD010000390         3889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_121245,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000390 DASSLD010000000
VERSION     DASSLD010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121245"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..549)
                     /locus_tag="VFK23_07100"
     CDS             complement(109..549)
                     /locus_tag="VFK23_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318884.1"
                     /translation="METRILSGANRYLLVLAIVLCVGLFSISRDVSAGQGALDGKTFV
                     VETGEKGKSGGDKDTLTFKDGNFHSAGCDQYGFGVGGYTSTVNGDTIQFEAVTTSPTK
                     GKMTWKGTVTGDKIEVAYVWVDASHWYKPNPKPLEKWAKGELKK"
     gene            complement(676..2328)
                     /locus_tag="VFK23_07105"
     CDS             complement(676..2328)
                     /locus_tag="VFK23_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7318885.1"
                     /translation="MSTTKSLTKSLLLVLLTAFLFVSSLEAHEEDTTPGKSSNEVLGK
                     VHFVTSCSPAVGRSFNRAVALLHSFWYAEAEKAFADVDSTDAGCAMAHWGVAMTYFHQ
                     LWAPPTPVDLKKGSSAAEEAVSVGAKTERERDYIQAIRIFYQDSAKLDHKTRAKAYEK
                     AMEQVYLRNPDDKEAAIFYALALDVTAPETDKTYAQQRKAGGILNKLAPQLPDHPGIA
                     HYLIHSYDFPPLATLGLPAARSYSRIAESSPHALHMPSHIFTRLGLWDESIPANLASA
                     EAAKNYVDRVHPGATSSERLHSMDYLEYAYLQGSQDGKAKALVEEVHGIGKVDWEAQA
                     SAYALAAIPARYALERHRWGEAATLTLYPADFPWQRFRYAEAIITFARAIGAARSGNM
                     MSAETDIERLAALRDEVAAKDTYWAGQIEIQRRTASAWLAYGKGDKEEALQLMQSAAG
                     LESATEKHPVTPGAVLPARELLADMLMELGRPAEALQEYEATLSISPNRFNSLYGAAN
                     AAAKAGDKEKARNLYTQFMFLCGHADSERPELVQAKLFLAPK"
     gene            complement(2649..3080)
                     /locus_tag="VFK23_07110"
     CDS             complement(2649..3080)
                     /locus_tag="VFK23_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318886.1"
                     /translation="MEEVYEEQVIMNANRTVNWMAIAAIVFGVMTVLTGGRALFGSLE
                     SRAGFGIAVPFVLWFNFLAGFVYIVAGAGLLLCRRWAVYNSLFLAVSTILVFLAFGVH
                     IIGGGAFERRTIGALTIRSIFWIAVTIVSMRAMKKTTNLES"
     gene            complement(3113..3466)
                     /locus_tag="VFK23_07115"
     CDS             complement(3113..3466)
                     /locus_tag="VFK23_07115"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HET7318887.1"
                     /translation="MPQLGRDVNDVGIIPTFAIGANGFVLGVVYRQAAEVVGFSKAAL
                     KVLPHGVFELPALLIAASYGLWLGVTVVRRMRGKESTLLRFHIEHAFRQYFAVVFPLL
                     IVAAAIETVLILRLQ"
     gene            complement(3625..3701)
                     /locus_tag="VFK23_07120"
     tRNA            complement(3625..3701)
                     /locus_tag="VFK23_07120"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3665..3667),aa:Lys,seq:ctt)
     gene            complement(3725..3799)
                     /locus_tag="VFK23_07125"
     tRNA            complement(3725..3799)
                     /locus_tag="VFK23_07125"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3764..3766),aa:Arg,seq:ccg)
     gene            complement(3810..3885)
                     /locus_tag="VFK23_07130"
     tRNA            complement(3810..3885)
                     /locus_tag="VFK23_07130"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3850..3852),aa:His,seq:gtg)
BASE COUNT          841 a         1179 c         1051 g          818 t
ORIGIN      
        1 acccggtgtt aatccggatt ccctttctct attccgatgg tgccgaaggg gggattcgta
       61 cccgcgcgag gttccctgca acgcttatgc aatggcagcc gagtcgaatt atttcttcaa
      121 ttctcccttc gcccattttt ccagaggctt cggattgggc ttgtaccagt gggaggcgtc
      181 gacccagaca taggccacct cgatcttgtc gcccgtcaca gttcccttcc aggtcatttt
      241 ccctttcgtc ggacttgtcg tgacggcctc gaactggatc gtgtctccat tcacggtcga
      301 tgtgtagcca ccaacaccaa acccatattg atcacatccc gcagaatgaa aatttccgtc
      361 cttgaaggtg agcgtatcct tgtctccccc gctcttcccc ttctctccgg tttcgaccac
      421 aaaggtcttc ccgtccaaag ccccctgacc ggcggaaacg tcccgggaaa tggagaatag
      481 accgacacac aggacgattg ccagaaccag caggtaacga tttgcccccg aaagaattct
      541 cgtttccata tcgtctcctt cctgcaactt ccaattgaaa ttgccataca gggttaatgg
      601 attcctccgg tgccgccata aaacccctca ataagctccc ttcaaccgcg ggtgaacagc
      661 gccatttccg gccgcttact tcggtgcaag aaacaacttc gcctgcacga gttccggccg
      721 ctccgagtca gcgtggccac acaggaacat gaactgcgtg tacagattcc tggctttttc
      781 cttgtcgccg gccttcgccg ccgcgttggc ggcgccatac aatgagttga aacgattcgg
      841 cgagatggag agcgtcgctt cgtactcctg cagcgcctcg gcaggcctac ccaactccat
      901 tagcatatcg gccagcaatt ctcgcgcggg aaggacagcg ccgggagtga ccggatgctt
      961 ctccgttgcg ctctccagcc cggccgcgga ttgcatcaac tgcagtgcct cctccttatc
     1021 tcccttcccg taggcgagcc acgcactcgc ggtgcgacgc tggatttcga tctggccggc
     1081 ccagtaggtg tccttggccg ccacttcatc gcggagcgct gcaagccgct cgatgtcggt
     1141 ctccgcggac atcatattgc cgctgcgggc ggcgccgatg gcccgagcaa acgtgatgat
     1201 ggcctcggca tagcggaagc gctgccacgg gaaatcggcg ggatatagag tcagcgtcgc
     1261 ggcctccccc cagcgatgac gttcgagggc atagcgggca gggatcgctg ccaaggcgta
     1321 tgccgacgcc tgggcttccc aatctacctt cccgataccg tgcacctctt cgaccaacgc
     1381 cttggccttg ccgtcctgtg agccctgcag gtaggcgtac tctaggtaat ccatcgaatg
     1441 cagccgttcc gaagacgtgg ccccagggtg cacccggtcg acgtaattct tcgccgcctc
     1501 cgcggatgcc aagttggcgg gaatcgactc atcccacaaa cccagacgcg tgaagatatg
     1561 cgatggcatg tgaagcgcat gcggggacga ttcggcgatc ctggagtaac tgcgcgcggc
     1621 gggcaaaccg agagtcgcca gtggcgggaa gtcgtagctg tgaataaggt agtgggcgat
     1681 gcccggatgg tcaggcagtt gcggcgccaa cttgttcagg attccaccgg cttttctctg
     1741 ttgcgcgtag gtcttgtcgg tttccggcgc tgtgacgtcg agcgcgaggg catagaagat
     1801 ggccgcctcc ttgtcatcgg gatttcgcag gtacacctgc tccatcgcct tctcgtacgc
     1861 cttggcgcgg gtcttgtggt cgagtttggc cgaatcctga tagaagatcc ggatggcctg
     1921 gatgtaatcc cgctctcgct cggtcttggc gcccaccgag acggcctctt ccgcggcgga
     1981 cgatcccttc ttcaaatcca ccggggtcgg cggcgcccaa agttggtgga agtaggtcat
     2041 ggccacgccc caatgggcca tggcgcaacc ggcgtctgta ctgtcgacat ctgcgaaggc
     2101 tttttccgcc tccgcgtacc aaaacgaatg gagaagcgcc actgcgcggt tgaaggacct
     2161 cccaacggcg ggactgcaag aggtcacgaa atggactttt cccaacacct cgttggaact
     2221 ctttccggga gtggtgtctt cctcgtgcgc ttccagggag gagacaaaaa gaaacgctgt
     2281 cagcaatacc aataacaacg atttcgttaa cgatttggtc gtgctcatgg gcacctcccc
     2341 tttcctcggc ccggcaatta cactctcagt agagtcggga aaggcaagcg ggacgatgta
     2401 agggaaacta cccgccaatc cataaatccc ctttccgaga agtcccaaag tttccggggc
     2461 aatcaattgg gattgtggga ttccttatgt tttccaatga gattctacgg ggaggagaga
     2521 gggttcctgt tcggaagagt ttctgctctt tacaagggga acgggctatt cgatttcccc
     2581 gcccaaaaag cgagatgttt gcacgggatt tgcagtccgt tcggctttcc gaaatccgta
     2641 aagatgtcct acgactccaa attcgtggtc ttcttcatcg cacgcatcga gacaatcgtc
     2701 actgcaatcc agaatatcga tctaattgtc aacgcgccga tggtccgcct ctcgaatgcc
     2761 ccgccaccaa taatgtgaac gccaaatgcc agaaagacga ggattgtgga aaccgctaag
     2821 aacagcgagt tatagacagc ccagcgtctg cacaggagaa ggcccgcccc ggctacgatg
     2881 taaacaaagc cagcaagaaa attgaaccag agcacaaagg ggaccgcaat tccgaaaccg
     2941 gcgcgcgatt ccaaactgcc aaacaatgct cgtccaccag ttaagactgt cataacaccg
     3001 aatacgattg cggcgatcgc catccaattt actgtacgat ttgcattcat gatgacctgc
     3061 tcctcataaa cctcttccac tatacaaggg gtttccccta tccataccgt tcctactgca
     3121 acctgaggat gaggaccgtc tctatcgccg ccgcaacaat caggagagga aacacgacgg
     3181 cgaagtactg acggaaggca tgctcgatat ggaatctgag gagcgtactt tcctttcccc
     3241 gcattctccg gacgacggtc acaccgagcc acaggccata ggaagccgca attagaagcg
     3301 ctggtaactc gaagacgcca tggggcagca ccttcaacgc tgccttcgaa aaacccacta
     3361 cctcagcggc ctggcgatac accacaccga ggacaaaccc attggccccg atggcgaatg
     3421 tcgggatgat ccctacgtcg tttacgtcgc ggcccagctg gggcacgctt cgtcccacgt
     3481 taccttgacc acttatagtc atgcccttcc cggaggggac agatcagcgg tcaatcggct
     3541 gggcgcagcg ctccaaaacg cgccgccagc gcgccgggac gaaaaaaggg gtcagaccga
     3601 agtggtctaa ccccttgatt ttgctggtgg gccatgtagg attcgaacct acaacctcct
     3661 gattaagagt cagctgctct accgggttga gctaatggcc cacgatggga aagaacctgg
     3721 atcttggcgc gcctgggagg aatcgaacct ccgcacccgg ctccggaggc cggtgctcta
     3781 tcccctgagc tacaggcgcc tgaagatgat ggggtgagtg acggggattg aacccgcgac
     3841 agccagagcc acagtctggt gctctgccaa ctgagctaca ctcaccgcc
//
LOCUS       DASSLD010000391         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_251894,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000391 DASSLD010000000
VERSION     DASSLD010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251894"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFK23_07135"
     CDS             <1..598
                     /locus_tag="VFK23_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7318888.1"
                     /translation="SKVRAVQPLEIGRDRISTVLVKVKDDMRPDRVALWIEHDIPGVK
                     ALPSDTVISTVRKQLSGLIRAILAISGILWLIVLLIMAFAFYMIVNERRREIGLLRAM
                     GANKGHISTIILTEASLLSAGGGAAGILLGFGLLASFKNPILHHLKLPYLFPSPVTFA
                     ALVAGALILSIMTGLLSGLLPSLAVLRMEPYDAIRREE"
     gene            646..966
                     /locus_tag="VFK23_07140"
     CDS             646..966
                     /locus_tag="VFK23_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318889.1"
                     /translation="MEAIQVSLILVAGIKIDNNLNKGRPMKRDYANNQRGEVIIGVMM
                     IIMCAVMLFGGMHMMHGGHRHSDDGNGRVEEKHDYRKVERHHPLNDNGEYRSARDEET
                     ESQQ"
     gene            complement(1092..>2285)
                     /locus_tag="VFK23_07145"
     CDS             complement(1092..>2285)
                     /locus_tag="VFK23_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET7318890.1"
                     /translation="LASAVFTLQPVGIMDPLSLLIRSFSVSVYPLFNYGVRSLFDAVY
                     AANPAGIAAVSEPVYAVLKKTVLSFEQSFYNQGFFIGMIFFVVIGLNLIEKRFWCKYL
                     CPLGALLGILSRFSLLKRSVSEGCTSCGVCATTCQGNAQPETKDHWRDTECLVCRNCD
                     DICPQNAVSFGFKPTGKVAAMDLGRRRVIASVVSGIVAVPLLRTTPVSKTGAQEPALL
                     RPPGALEEREFLKRCVKCGECMKVCTTNGLQPTLFEAGVEGIWSPVLVPRIGYCEYRC
                     TLCGQVCPTGAIKRLSLEEKAKVRIGLAMIDKGRCLPWTHGRPCIVCEEVCPTSKKAI
                     WLEEARVRDRQGKVVVVKQPHVDLELCIGCGICENKCPVLGRPAIYVSNVGESRSKEN
                     RLLLT"
BASE COUNT          519 a          652 c          657 g          457 t
ORIGIN      
        1 ctcaaaggtc cgggcagtcc agccgctgga gatcggaagg gacaggatat cgaccgtcct
       61 tgtgaaggta aaagacgata tgagaccgga ccgggttgcg ctgtggatcg aacacgacat
      121 tccgggagtg aaggccctgc catcggatac ggtcatcagc acggtgagaa agcagctctc
      181 ggggctgatc agggccattc tggccatcag cggcatcctg tggctcatcg tccttttgat
      241 catggcattc gcattctata tgatcgtgaa cgagcggcgc agggagatcg gacttctgcg
      301 cgccatgggc gcaaacaagg gccatatatc gacgatcatc ctgactgagg catcgctcct
      361 ctcggcgggc ggcggcgcag ccggaatcct gctcgggttc gggctgctcg cgagcttcaa
      421 aaacccgata ctccatcacc tgaagcttcc ctatctattc ccgtcgccgg tgacgtttgc
      481 cgcactcgtc gcgggagcgt tgatcctgtc catcatgaca ggcctgctgt cggggctcct
      541 cccgtccctt gccgtactgc gcatggagcc ctatgatgcg atcaggagag aagaatgaac
      601 tagtgcccat aaacacgaat attgtcggat gttgagggga actacgtgga agcgatacag
      661 gtgtcgttga tcctggtcgc cggaattaaa attgacaata atctaaataa ggggcgacca
      721 atgaagcggg actatgcgaa taatcagagg ggcgaagtta ttatcggtgt catgatgatc
      781 ataatgtgtg cggtgatgct tttcggaggc atgcacatga tgcacggagg acaccggcat
      841 tcggatgacg ggaacggaag ggttgaggag aagcacgatt accggaaagt ggaaaggcat
      901 cacccgctga acgacaacgg ggaataccgt tccgcccgcg atgaagaaac agaatctcaa
      961 caataagcat ccgaattcag ggaagatgta gacatgaccg ggcggtttaa gatcaagacc
     1021 gttctcagga cgactttggc gacctcctat aagccccctg tcccggccta cccgcgattt
     1081 ggccgaagat ctcacgtcag cagcagccgg ttttctttcg acctgctctc acccacgttc
     1141 gacacgtaaa tagccggtct tcccaaaacc gggcacttgt tctcgcagat cccgcaccca
     1201 atgcatagtt cgagatccac gtgcggctgc ttgacgacca cgaccttacc ctgccggtcc
     1261 ctcacccgcg cctcttcgag ccagatggcc ttcttcgagg tcggacagac ctcctcgcat
     1321 acgatgcatg gcctgccgtg ggtccaggga agacatctgc ccttgtcgat catggcgagc
     1381 ccgatcctta ccttcgcctt ttcttcgagc gacagccttt tgatcgcgcc cgtggggcag
     1441 acctggccgc aaagtgtgca gcggtactcg cagtacccga tgcgcggtac gagcacggga
     1501 gaccagatac cctcgacgcc cgcctcgaaa agcgtcggct gaagcccgtt cgtggtgcag
     1561 accttcatac actccccgca cttgacacag cgtttcagaa attccctttc ttccagggcc
     1621 ccgggaggcc gaagcagcgc cggttcctgc gcccctgttt tcgagaccgg cgttgttcga
     1681 agaagcggca ccgccacgat accggagact acggacgcaa tcacgcgccg ccttccaaga
     1741 tccatggccg cgacctttcc tgtaggtttg aacccgaagc tcacggcgtt ctggggacat
     1801 atgtcgtcgc agttcctgca gacgaggcac tccgtgtccc gccagtggtc ctttgtttcg
     1861 ggctgggcgt tgccctggca tgtggttgcg cagacgccgc aggaggtgca gccttcactt
     1921 accgatctct tcaggagaga gaaccgggac aggatgccca aaagcgcacc cagggggcag
     1981 agatacttgc accagaaccg cttctcgatg agattcaggc cgatgacgac gaagaatatc
     2041 atcccgataa aaaagccctg gttatagaat gattgctcga aggaaagcac ggtttttttg
     2101 agaactgcat acactggttc ggaaacggcg gcgatgcctg ccggatttgc cgcatagacc
     2161 gcgtcgaaca acgatcgtac gccgtaattg aacaacggat agacgctcac ggagaacgag
     2221 cgtatcaaga gcgagagcgg gtccatgatg cccacgggct gaagcgtaaa gacggccgag
     2281 gcaag
//
LOCUS       DASSLD010000392         3153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_982295,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000392 DASSLD010000000
VERSION     DASSLD010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982295"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VFK23_07150"
     CDS             complement(<1..1080)
                     /locus_tag="VFK23_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318891.1"
                     /translation="MAEEIRQPEALSVEIQDIMRTLISAIRTVKLYPPNNPVYSQSVK
                     KSYEALAHYLAESPEYAAGVQKTYFTYRQAPVGKDGQLNKATAQDLFMKGVREITVSA
                     GVTEEELLELYRTLALSSEELSLKNGIASILWEKGASHITVIEAGLDDVITTKAEGGW
                     EDKTPSDNISGAGKKPVVQTRRTLVLGDIMTDPALFGASMVELARQTKAAFESVEDRL
                     FTLYQEAGRKIQEEQPERGDELFDGLAKSILSLSRPYREILIGGKLYGSLDAEIAEDR
                     DMELDQQLPSAAHEVRTGRFSETWTVQQVATLLKKSSSKKSGPPTPPSTPAALQPTPL
                     PPDLTSIANELAEYTPNEMEQLKALG"
     gene            complement(1094..2305)
                     /gene="xseA"
                     /locus_tag="VFK23_07155"
     CDS             complement(1094..2305)
                     /gene="xseA"
                     /locus_tag="VFK23_07155"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET7318892.1"
                     /translation="MKSHILTVTQLTSQIKNLLEGTFPDVWVEGEVSNLSIPQSGHAY
                     FTLKDELSQVRAVLFRSSQRFLKFTLQHGIQVICRGRVSLYEPRGEYQLVVDYVEPKG
                     IGALQLAFEQLKARLEKEGLFALDHKKPLPALPRRIGIVTSPTGAAIRDMLRVIKRRH
                     PNMHILIYPVPVQGAEAAPSIVEALRYFNSERNVDVVIAGRGGGSLEDLWAFNEEAVA
                     RAIYTSSIPVISAVGHETDYTIADFAADLRAPTPSAAAEMVVEREDTIREGIRTLEAR
                     LVRSVNRTLELVRARIRESARLIGDPRRRLEQYGQRVDDMLHRMALELRHRLRRERAG
                     LTALTSGLDHLSPLGILSRGYSITRKLPTGSILKNAADVRSGDLIGTRLHRGEVLSRV
                     EKQDFDTQQEL"
     gene            complement(2365..3153)
                     /locus_tag="VFK23_07160"
     CDS             complement(2365..3153)
                     /locus_tag="VFK23_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HET7318893.1"
                     /translation="MGGFLKVLFIGDIIGEPGRKTVRANLRSLVEAHGPDLVIANGEN
                     AAGGFGITSEIAEELLSLGIHVLTSGNHVWDKKEIEPYLAKQDRLIRPANYPEGNPGY
                     GSVVISTGSGKAAVLNLEGRVFMSNLEDPFRAAVREIEKLMKETRVVILDFHAEATSE
                     KTALAWHVDGKASAVVGTHTHVQTADERILPSGTAFITDAGMTGPTDSVIGVKKEQAI
                     GRFLYQTPHRFEIPKGPSHLDAVVIDIDAQTGKAKSIERIHMKS"
BASE COUNT          534 a          938 c          963 g          718 t
ORIGIN      
        1 gcccagggcc ttgagctgct ccatctcgtt cggcgtgtac tcagccagtt cattcgcgat
       61 tgaggtaagg tccggtggga gcggggttgg ctgcagtgcg gcgggtgtcg aaggaggcgt
      121 cggcggcccg ctttttttgg atgaggattt tttcaggagt gtcgccacct gctgcacggt
      181 ccaggtctct gaaaaacgtc ccgtccgcac ctcatgggcc gcgctgggca gttgctggtc
      241 gagctccata tcccggtctt ccgcgatttc cgcgtccagg cttccgtaca gcttgccgcc
      301 gatgagtatt tcccggtagg ggcgtgaaag ggacaggatc gacttggcca gaccgtcgaa
      361 gagctcatcg ccccgttccg gctgctcctc ttgtatcttt ctcccggctt cctgatagag
      421 ggtgaagagg cggtcttcca cgctttcgaa agccgccttt gtctgtctcg caagctcaac
      481 catgctggcg ccgaacagcg caggatccgt catgatgtcg cccaggacaa gcgtgcggcg
      541 ggtctggacg accggcttct tccccgcccc ggaaatgttg tcggaaggcg ttttgtcttc
      601 ccatcctcct tctgccttgg tcgttatgac atcgtcgagc cccgcttcga tcaccgtgat
      661 gtgggaagcg cctttttccc agaggatcga cgcgatgccg tttttcaggg acaattcttc
      721 cgatgacagg gcgagggtgc gatacagctc caacagttcc tcctcggtca ccccggcgct
      781 gacggtgatc tcccgcaccc ctttcatgaa gaggtcctgg gccgtggcct tgtttaactg
      841 accgtctttt ccgaccgggg cctgccggta ggtgaaatag gtcttctgga cgccggcggc
      901 atattccgga gattccgcga gatagtgagc gagcgcttca taggattttt ttactgactg
      961 ggagtagacg gggttgttgg gaggatagag cttcacggtc ctgatggctg agatcagggt
     1021 ccgcatgatg tcctggattt caacggaaag ggcctcaggt tgtctgattt cttccgccat
     1081 ggtcagtagg gtatcacagt tcctgttggg tgtcaaaatc ctgtttttcc actctcgaca
     1141 ggacctcgcc ccggtgcaat ctggtgccga tcaaatctcc ggaccggaca tcggccgcat
     1201 tcttgaggat tgagcctgtc ggaagctttc tcgtgatact gtatcccctc gacagaatgc
     1261 cgagcgggct caaatggtcc agacccgaag tcagtgccgt cagacctgcc cgctccctcc
     1321 gcaggcggtg ccggagttcg agcgccatgc gatggagcat atcatccacg cgctggccgt
     1381 actgttccag ccttctgcgc ggatctccga tgaggcgcgc gctttccctg attctcgcgc
     1441 gcacgagttc cagcgtccgg ttcacgctcc tgaccaggcg cgcctccagg gtccggatgc
     1501 cctcccggat cgtgtcctcg cgttccacca ccatttccgc ggctgcggac ggcgtcgggg
     1561 cgcgcaaatc tgcggcgaaa tcggctatcg tatagtctgt ctcgtgcccg accgcggaga
     1621 tgaccggtat cgaggaggta taaatcgccc gtgcaaccgc ttcctcattg aaggcccaca
     1681 ggtcttcaag ggagccgccg ccgcggcccg cgatcacgac atcgacattc cgttcggagt
     1741 tgaaataccg gagcgcctca acgatcgagg gcgccgcttc ggcgccctgc accggaacgg
     1801 gatagatgag gatgtgcatg ttcggatggc gccgcttgat cacgcgcagc atatcacgga
     1861 tggcggctcc ggtcggcgat gtgacgatgc cgatgcgcct ggggagggcc ggcaacggtt
     1921 tcttgtgatc gagggcgaac aggccctcct tctcaagccg ggccttgagt tgttcaaacg
     1981 cgagttgaag cgcgccgatt cctttgggtt cgacataatc gacgacgagc tggtactccc
     2041 cgcgcggctc gtagaggctc accctgcccc ggcagatgac ctggatacca tgctggagtg
     2101 tgaacttgag gaaccgctgg gacgagcgga aaaggacggc ccggacctgg ctcagttcgt
     2161 ctttcagggt gaaatacgcg tgaccggact gcggaatgct gaggttcgat acctcgccct
     2221 ccacccagac gtcggggaac gtcccttcga ggaggttctt gatctgactg gtaagctgcg
     2281 tgacggtcag gatgtgagac ttcatagttt cgggttgcag gacgggaatg cggaatgtac
     2341 catcgcgatc cgcaactacg cgatttacga cttcatgtgt attctttcta tcgacttcgc
     2401 cttcccggtc tgagcgtcga tgtcgatgac gaccgcatcg agatgcgacg gccccttggg
     2461 gatctcgaac cggtgcggcg tctgatagag gaaccgcccg attgcctgct ctttcttgac
     2521 gccgatgacc gagtcggtgg gccccgtcat gcccgcgtcc gttatgaaag cggtgcccga
     2581 cgggaggatg cgttcgtcag cagtctggac gtgggtatgg gtccctacaa cggcgcttgc
     2641 cttcccgtcg acgtgccatg ccagagccgt tttctccgac gtggcctccg cgtggaaatc
     2701 gaggattacc acgcgtgttt ccttcatgag cttttcaatc tcgcggactg cggcgcggaa
     2761 ggggtcttcc aggttagaca tgaagacccg gccttcgaga tttaacaccg cggccttgcc
     2821 gctccccgtg gagatgacga cgctcccgta cccggggttg ccttcgggat aatttgccgg
     2881 cctgataagc ctgtcctgct tggcgagata gggttctatc tccttcttgt cccagacatg
     2941 gtttcccgat gtcaggacgt gtatgcccag tgacaggagt tcttcggcga tctcggaggt
     3001 gatgccgaag cccccggcgg cgttctctcc gttcgcgatc acgaggtcgg ggccgtgcgc
     3061 ctccacgagg ctgcgcaggt ttgcccggac cgtcttccgt cccggctcgc ctatgatgtc
     3121 gccgatgaat aagaccttca agaagccccc tat
//
LOCUS       DASSLD010000393         2453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_231261,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000393 DASSLD010000000
VERSION     DASSLD010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231261"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFK23_07165"
     CDS             complement(<1..430)
                     /locus_tag="VFK23_07165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7318894.1"
                     /translation="MSNETNPLAGLSYNKGVTGIPPADDDAGLVTRSRQGDLDAFEQL
                     VTKHQKKMLNIAYRLIGDYDEACEVVQDAFVSAHRNIRTFRGDSKFTTWLTAITLNLS
                     KNRLKQLISRKGHEAYSLDDPVETNDGKVTIDPLSKDPSVL"
     gene            526..732
                     /locus_tag="VFK23_07170"
     CDS             526..732
                     /locus_tag="VFK23_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258239547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:562..564,aa:Sec)
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HET7318895.1"
                     /translation="MASKVIIYGKQGUPYTVKAREAYGDKAEFFDVKMDRAKLEEMLK
                     LSMGARKVPLIVEDGKVAIGYGGS"
     gene            836..1393
                     /locus_tag="VFK23_07175"
     CDS             836..1393
                     /locus_tag="VFK23_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HET7318896.1"
                     /translation="MILDKFFLDKEGTVLLIVDIQEKLAAVMKEKDKVVKNNLHLVEL
                     AKMIGMPVMVTEQYPKGLGTTVPEIREALPFYRPVEKMTFDCCGQPTFLEELKEHNKS
                     NVVLTGMETHICVLQTCIGLLKGGINVHVVQDAICSRTKENWRAGNEFMRDAGAVLTC
                     TETVLFQLLRVAGTEEFKAVSRRIK"
     gene            1504..>2453
                     /locus_tag="VFK23_07180"
     CDS             1504..>2453
                     /locus_tag="VFK23_07180"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET7318897.1"
                     /translation="MAEEPIPEEVKTVIKETFFKELKNDVALEVYTRDGVNDKFNEAT
                     VTLIMALAGLSDKLKVSFHKVGDDSSVKRNVHRSPTVLIAPDKYHLRYTGAPLGEEGR
                     SLLVSILMASTGNSILTAATMDKINGLKEKRDIQVFVSPTCPYCPQQALSAFSAAILK
                     PDLVSAEVIEIYENQDLAENLGSLSVPQTFINGIFTGMGLQPEEFFAESLLSLKEPEY
                     ISEQLSAEPVEKDLVIVGAGPAGLTAAIYAVRAGLTAVVIEREVIGGQIAITPVVENY
                     PGYMRIGGKSLVDLISQQAVQYADIHIGEQVKEISQDKQD"
BASE COUNT          608 a          627 c          677 g          541 t
ORIGIN      
        1 ccagcacaga ggggtccttt gacagcgggt cgatggtcac cttaccatcg ttggtctcta
       61 ccgggtcgtc caaggaatag gcttcatggc ccttccggga tatcagctgc ttcaaccggt
      121 ttttcgaaag gttgagtgtg atggccgtga gccaggttgt gaacttcgaa tcgccccgga
      181 acgtcctgat attcctgtgg gccgatacaa aagcgtcctg gaccacttcg cacgcctcgt
      241 catagtctcc aatcagccga taggcgatgt tcaacatctt tttctggtgc tttgtcacga
      301 gctgttcaaa ggcatcaagg tcaccttgcc gagacctcgt gacaaggcct gcatcgtcat
      361 ccgccggcgg aatgccggtc actcctttat tataagacaa tccggcaagg ggatttgttt
      421 cattgctcat gatgaaacta attctatcgt aaaaggttta cttttgcact cgcaaaatga
      481 taatatataa ttatcgaaat tacctttgag tagaggaggt cagccatggc aagcaaggtc
      541 atcatttacg ggaaacaggg ttgaccgtat acagtcaagg ctcgtgaagc ctacggagac
      601 aaggctgaat ttttcgatgt gaagatggac agggccaagc tcgaagagat gctgaagctc
      661 tccatgggtg cgaggaaagt accgctcatc gtagaagacg gaaaggtcgc tatcggctac
      721 ggcgggtcgt gaggcgtata gcagaccggc aggctgctgg tgaagaacaa ttgcgaagcg
      781 tggaataaat tgagcaagac ataattcatt ttattttaag gagaagactg gcattatgat
      841 tctggataaa tttttccttg ataaggaagg cacggtgctc ctgatcgtcg atatccagga
      901 aaagctcgcc gcggtgatga aagagaagga caaggtggta aagaacaacc tgcacctggt
      961 cgagctcgca aagatgatcg gcatgccggt gatggtgacc gagcagtatc ccaaggggct
     1021 cggtacgact gtgccggaga tccgcgaggc actgccgttc tatcgccctg ttgagaagat
     1081 gacctttgat tgctgcggtc agcccacctt tcttgaggaa ttgaaagagc acaataaaag
     1141 caatgtcgtc ctgacaggca tggaaacgca catctgcgtg ctccagacct gtatcgggct
     1201 cttaaagggc ggaatcaatg tccacgtagt gcaggacgcg atatgttctc gaaccaagga
     1261 gaactggaga gcggggaacg agttcatgcg cgacgccgga gcagtcttaa cctgcaccga
     1321 gacggttctg ttccagctcc tcagggtcgc gggcacggag gagttcaagg ctgtttcgag
     1381 gaggatcaag taatcatgca agaatgcaga aaggaaccct ttcatcggtt ctgacgcttg
     1441 cattctaaag gcggatttaa ccgtgtcgtc ggcgatttca acagtccttc acgaggtgac
     1501 accatggcag aagaacccat tccggaggaa gtcaaaacag ttatcaagga aacgttcttc
     1561 aaagaattga agaatgacgt cgcgcttgag gtctatacga gggacggcgt gaacgacaaa
     1621 ttcaacgagg ctaccgtcac gcttatcatg gccctggcag gactgagcga caagctcaag
     1681 gtctctttcc acaaagtcgg tgatgacagt tccgtgaagc gcaacgtcca ccgttctcca
     1741 acggtgctca ttgcacctga taaataccac ctacgctaca ccggcgctcc gctcggcgaa
     1801 gaaggccggt cgctcctcgt ttccatcctc atggcttcca cgggcaatag tatcctgacc
     1861 gctgcgacca tggacaagat caacgggctc aaggaaaaac gggatatcca ggtcttcgtc
     1921 agccccacgt gcccttactg cccgcagcag gcgctctctg ccttctccgc cgcgatctta
     1981 aagccggacc tcgtatctgc ggaggtgatc gagatctacg agaaccagga cctggctgaa
     2041 aacctcggct cgctttccgt gccgcagacc tttattaatg gcatcttcac gggtatggga
     2101 ctccagcccg aggagttttt cgctgagtcg ctcctctcgc ttaaagagcc agagtacatc
     2161 tctgagcagc tatcggcgga gcccgtcgag aaggatctcg tcatcgtagg cgccggccct
     2221 gccggtctca cagccgctat ctacgccgta cgcgccgggc ttactgctgt cgtgatagag
     2281 cgggaggtca tcggcggaca gatcgccata acgccggtgg tcgaaaacta tcccggttac
     2341 atgcgcatcg gaggtaagag tcttgtggat ctcatctcac agcaggcggt ccaatacgcg
     2401 gacatccaca tcggcgaaca ggtgaaggag atttcacagg acaagcagga cag
//
LOCUS       DASSLD010000394         2690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1283151, whole genome shotgun sequence.
ACCESSION   DASSLD010000394 DASSLD010000000
VERSION     DASSLD010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283151"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2391)
                     /locus_tag="VFK23_07185"
     CDS             complement(<1..2391)
                     /locus_tag="VFK23_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET7318898.1"
                     /translation="MSEGGYHDMMFEKFTERGRKVIVYAREEAERLQNDYLGTEHILL
                     GTLREEDGIPVAVLRKMGIDVDQIRVEVERNLPSSGNTLTFGDIPFTPRAKKVLEYAV
                     EEARLLGHNYIGSEHLLLGLIREEEGIGGKILRSFGVNLLGSRQLVINYLRRAATQVS
                     AKKSPTPALDEFSRDLTQLAKSGKLDPVIGRDQEIDRVLQILSRRTKNNPVIIGEPGI
                     GKTAIVEGLAQRIVNNDVPENIMNRRVVSLDLGSLIAGTKYRGQFEERLKIVMKEIVQ
                     AENVILFIDELHTLVGAGAAEGSIDASSMLKPALARGEIQCIGATTLDEFRKHIEKDG
                     ALKRRFQPIHVQPPSMEETVNIIKGLRPKYEAHHKVKITDEAINAASRLSDRYITDRF
                     LPDKAIDVIDEAGAKAKLNRYTYPAEMRTIEKKLKKLEQEKNLFSRIKDYVRVESIHE
                     EEDRLRESLDGIHKDWKDNQEKNLPVITEEDIASVVSHITGIPLSRLEEKEASRLLRM
                     EDELHKRIVGQDEAIDAVSRAIRRSRVGLKTRRQPIGSFIFLGPTGVGKTELARTLAS
                     FLFDTEDALIRVDMSEYMEKFSSSRLVGSPPGYVGYDDGGQLTEKIRRRPYSVVLFDE
                     IEKAHPDIFNMLLQVLDDGFMTDSFGRRVDFRNTVIIMTSNLGARMIDKDTTLGFQQD
                     SSKTQYEKMKDNVISELKRSFNPEFLNRIDEVVVFHPLVQEHLVKIVDMLVGELNAHM
                     IADRSVTLEVTAEVKEWLIRENYQPTYGARPMRRAIQKHIADPLSEEILRGRFKDIR"
BASE COUNT          476 a          768 c          777 g          669 t
ORIGIN      
        1 ccttatgtcc ttgaaccgtc cgcggaggat ttcctcggaa agcgggtctg cgatatgctt
       61 ctggatggcg cggcgcatcg gtctggcgcc ataggtgggt tgataatttt cgcggatgag
      121 ccattccttc acttccgccg tcacttccag ggtgacgctc cggtcggcga tcatatgggc
      181 attcaactcg cccaccagca tgtccacgat cttcaccaga tgttcctgga cgaggggatg
      241 gaatacgaca acctcgtcga tcctgttcag gaactcggga ttgaatgacc gcttcagctc
      301 cgagattacg ttgtccttca tcttttcgta ctgcgtcttg ctgctgtcct gctggaaacc
      361 gagggtcgtg tctttgtcga tcatcctggc gccgaggttt gacgtcatga tgatgacggt
      421 gttgcggaaa tccacccgcc tgccgaagct gtccgtcatg aacccatcgt cgagcacctg
      481 gagcaacatg ttgaagatgt ccggatgggc tttctcaatc tcgtcgaaca gcacgactga
      541 ataaggtctt cgcctgatct tttccgtcaa ttggccgccg tcgtcatatc ccacgtagcc
      601 gggaggagag ccgacgagcc gcgaggaact gaatttttcc atgtattcgg acatgtcgac
      661 gcggatgagc gcgtcctcgg tgtcgaagag gaacgaggcc agggtcctcg cgagttcggt
      721 cttgccgacg cccgtcggtc ccaggaagat gaacgagccg atgggctgtc tgcgagtctt
      781 gaggcctacc cgggagcggc gtatggcccg tgaaacggca tcgatagcct catcctggcc
      841 cacgatgcgc ttgtgcagtt cgtcctccat gcgcagaagg cgggacgcct ctttttcttc
      901 gagccgggag agggggatgc cggtgatgtg ggaaaccacc gaggcaatgt cctcttctgt
      961 tatgacgggg aggttctttt cctggttgtc cttccagtcc ttgtgaatgc cgtcgagcga
     1021 ttcgcggagc cggtcctcct cttcgtggat cgactccacc cggacgtaat ccttgatccg
     1081 tgagaagagg tttttctcct gctcgagctt cttcagcttt ttctcgatgg tccgcatttc
     1141 ggcggggtac gtataccggt tcagctttgc cttggccccg gcttcgtcga tgacatcgat
     1201 tgctttgtcg ggaaggaacc ggtcggttat atagcggtcc gagaggcgcg aggccgcgtt
     1261 aatagcctcg tcggttatct tgaccttgtg gtgcgcttca tacttcggcc gaagcccctt
     1321 gatgatattg accgtctcct ccatgctggg tggctgtacg tggatgggct ggaaacggcg
     1381 tttgagcgcg ccgtcctttt cgatatgttt cctgaattca tcgagggtcg tggcgccgat
     1441 gcactggatt tcgccgcgcg caagggccgg cttcagcatg ctcgaggcgt cgatcgaccc
     1501 ctctgccgct cccgccccca ccagcgtgtg caactcgtcg ataaacagga tgacgttctc
     1561 cgcctgcacg atctccttca tcacgatctt caggcgctct tcgaactggc cgcggtactt
     1621 cgtccccgca atgagcgatc cgaggtccag agaaacgacg cgccggttca tgatgttctc
     1681 ggggacgtcg ttgttgacga tccgctgcgc aagcccctcg acgatcgcag tctttccgat
     1741 gccgggctcg ccgatgatga cgggattgtt cttggtccgc cgggacagga tctggaggac
     1801 gcggtcgatc tcctgatcgc gcccgatcac cgggtcgagc ttgccgctct ttgcaagctg
     1861 cgtcaggtcc ctgctgaatt cgtcaagggc gggggtgggg ctctttttcg cgcttacctg
     1921 ggtcgcggca cggcgcaggt agttgatcac cagctgccgg gacccgagaa ggttgacgcc
     1981 gaagctgcga aggatcttgc cgccaattcc ttcctcctcg cggatgagcc cgaggaggag
     2041 atgttcgctt ccgatataat tgtgcccgag gagccttgcc tcctcgacgg cgtattcgag
     2101 gactttcttc gcgcgggggg taaaggggat gtcgccgaag gtaagggtgt ttccgctcga
     2161 gggcaggttt cgctcgacct caacgcggat ctggtcgaca tcgattccca ttttgcggag
     2221 cacggcgacc gggatgccgt cttcttcgcg gagcgtcccg agcaggatat gctccgtgcc
     2281 cagataatcg ttctgcagcc gctcagcctc ttcgcgcgcg taaacgatta cctttcttcc
     2341 ccgttccgta aacttttcga acatcatatc gtggtatccg ccttcagaca ttctatgaaa
     2401 tccgattttt ctgctctccg ctgactaaaa tatactccat tccgctcgat aaagtcaaga
     2461 acccttttgg taactgcaat acgtgcgttt cgcgcatcgc ttgccgattc tatcacacat
     2521 cgggaaggcc tcgtgcagca taaaaattca aattggtgct tgactcggtt tgggctttgc
     2581 gctagactcc ggcagttcat atcgtcaaaa acgcccgtcg cgagggagag ccaaagtatt
     2641 tatgccgaac ttaaaaccag cacttgttgc agtgatgctt tttgcggcgc
//
LOCUS       DASSLD010000395         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_732030,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000395 DASSLD010000000
VERSION     DASSLD010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732030"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..549)
                     /gene="pgsA"
                     /locus_tag="VFK23_07190"
     CDS             complement(1..549)
                     /gene="pgsA"
                     /locus_tag="VFK23_07190"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET7318899.1"
                     /translation="MKIFNVPNMLSLSRILSVPVFILLMLEPSPVRALAAGVVFSLAS
                     ATDWLDGYLARKWGQVTKVGKLLDPIADKILIMSALVTLVEIRSDVVPAWIAIIIIGR
                     EFAVTGLRAIASSDGIVIPAETVGKYKVGAQITAVLSLLLDYYMTKEWMRDLGVLALW
                     AAMVLSVYSAVQYFRTYWKKLS"
     gene            complement(546..1451)
                     /locus_tag="VFK23_07195"
     CDS             complement(546..1451)
                     /locus_tag="VFK23_07195"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7318900.1"
                     /translation="MSEWALRTRKRLTRLFLIWMTGCLRLFSRVMPYRAGICAGGVFG
                     FVSYYLLRRERTRAHEHLEVAFGEKGPAWIRRTARRSFVHLGRSQFEVMMMSSRRLTR
                     VVEVRGEEHFRAALARGKGVVFVTGHIGNWELMGHAVAAMNPGGLSVVAAPIEPKQVN
                     DMIVDLRAGMGVRTILRNRPGAAKELIRIFRENRVLGILIDQDTDVESAFVEFMGRPA
                     WTPTAAASMAIKFGAPLLFGYDHRDGDGTHTVIIEGPLELIRTGDHEKDVVANTAMLT
                     GKIEKAIRKDPEQWVWMHRRWRRQP"
     gene            complement(1448..1966)
                     /locus_tag="VFK23_07200"
     CDS             complement(1448..1966)
                     /locus_tag="VFK23_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HET7318901.1"
                     /translation="MKKSKTSIASKAKKIKLLLLDVDGVLTDGSIILDCRGNEQKAFH
                     VRDGHGIKLAQRAGITVGLITGRKSEVVNIRARELGIEEVHQGAHEKIAVYESLIAKY
                     GVSDVEVAFMGDDVVDLDILKRAGLAATVADADPAVRPHADLITRAAGGRGAVRELIN
                     LLLKYQTKRLYS"
     gene            complement(1963..>2508)
                     /locus_tag="VFK23_07205"
     CDS             complement(1963..>2508)
                     /locus_tag="VFK23_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7318902.1"
                     /translation="ACPLGLAPTASTTASLAMGDALAVALLNRRGFKQEDFACFHPGG
                     ALGRKLLLRVRDLMHTGGDVPVVSEMTLLKDAIYEISSKKMGITSVVDGSGKLTGVIS
                     DGDLRRWMERTEKSGENLLAKKAGDIMTKNPKVANKDSLAAEAVAIMEKNSITCLIVA
                     DASSRPEGVIHLHDLLKAGVV"
BASE COUNT          446 a          800 c          750 g          512 t
ORIGIN      
        1 tcagctcagt ttcttccaat acgtcctgaa atactggacc gcggaataga ccgacaggac
       61 catggcggcc cagagggcaa gaactccaag gtccctcatc cattcctttg tcatatagta
      121 atcgaggagg agcgaaagca ccgcggtgat ctgggcgcct accttgtatt tccccacggt
      181 ctccgccggg atgacgatcc cgtcgctcga agcgatcgcg cgaagcccgg tgacggcaaa
      241 ttcccgtccg atgattatga tcgcgatcca ggcgggaacg acgtccgatc gaatctcgac
      301 cagagtgacc agcgcggaca tgatcagaat cttatcggcg atggggtcga ggagcttgcc
      361 gaccttggtc acctgccccc acttgcgcgc gaggtatccg tcgagccagt ccgtggccga
      421 ggccagggaa aagacgaccc ctgcggcgag cgcgcggacg gggctgggtt ccagcatgag
      481 caggatgaat acgggcaccg aaaggattct gctgagcgag agcatattcg gcacgttgaa
      541 tatcttcacg gctgcctcct ccaccggcgg tgcatccata cccattgctc cgggtccttc
      601 cgtatggcct tctctatctt ccccgtcagc atggccgtgt tggcaacgac gtccttttca
      661 tggtcgcccg tcctgatcag ttccagcggg ccctcgatga ttacggtgtg cgtgccgtcc
      721 ccgtcccggt gatcgtatcc gaacaggagc ggcgctccga acttgatcgc catggaagcg
      781 gccgccgtcg gcgtccaggc cggcctgccc atgaattcga cgaaagcgct ttccacgtcc
      841 gtgtcctggt cgatcaggat gcccaggacg cgattttccc tgaatatgcg gatcagttcc
      901 ttcgccgctc ccggcctgtt gcgaaggatc gtccgcacgc ccatgcctgc gcgcaggtcc
      961 acgatcatgt cattgacctg cttgggctcg atgggagcgg ccaccacgct gagtcctccc
     1021 ggattcatcg ctgcgactgc gtggcccatc agctcccagt tccctatatg gccggtgacg
     1081 aacacgacgc cttttccccg ggcgagcgcc gcgcggaagt gttcttcccc ccggacctct
     1141 acgacccgcg tcaggcggcg ggaggacatc atcatgactt cgaactggga ccgacccagg
     1201 tggacgaagg atcttcgggc cgtgcgccgt atccacgccg gtcccttctc cccgaaagcg
     1261 acttccaggt gctcatgggc ccgcgtccgt tcacggcgca gcaggtagta cgagacaaat
     1321 ccgaacacgc cgcccgcgca gataccggcg cggtacggca ttacgcgaga gaaaagccgg
     1381 agacagccgg tcatccagat caggaaaaga cgggtcaggc gctttctcgt ccgaagtgcc
     1441 cattcactca tgagtagagc cgtttggtct ggtatttcag aaggaggttg atgagctcgc
     1501 gcacggcccc cctgcccccg gcggcccgtg tgataaggtc ggcgtgcggc cgcacggcgg
     1561 ggtccgcgtc ggcgacggtt gcggccaggc cggcgcgttt caggatatcc aggtcgacga
     1621 cgtcgtctcc catgaaggcg acctcgacgt cggacactcc gtactttgcg atgagggact
     1681 cgtaaaccgc gatcttttca tgggcgccct gatggacttc ttcgatgccc agttcccgcg
     1741 cacggatgtt cacgacctcg gatttccgcc cggtgatgag accgaccgtg attcccgcgc
     1801 gctgggcgag cttgattccg tggccgtcgc gcacgtggaa ggccttctgc tcgtttcccc
     1861 ggcagtccag gatgatcgac ccgtcggtca ggaccccgtc cacgtcgagc aggaggagtt
     1921 tgatcttctt tgcctttgac gcgatgctgg tctttgattt tttcacacca cccccgcctt
     1981 cagcagatcg tggagatgaa tgaccccctc cggcctcgat gaggcgtcgg caacaatgag
     2041 gcaggtgatg gaattctttt ccatgatggc cacggcctcg gcggccagcg agtctttgtt
     2101 tgcgaccttc gggtttttgg tcatgatatc acccgccttt ttcgcgagca ggttttcgcc
     2161 cgacttctcg gtcctttcca tccagcgcct gagatcgccg tcggagatca cgccggtcag
     2221 tttacccgac ccgtcgacga cggaggtgat ccccatcttt ttcgacgata tttcgtagat
     2281 ggcgtccttg agaagggtca tctcggaaac cacgggaaca tccccgcccg tatgcatgag
     2341 gtccctgacc cggagcagaa gcttcctgcc gagtgcgcct ccgggatgga agcaggcgaa
     2401 gtcctcctgt ttaaagcccc tcctgttgag gagcgccacg gccagggcgt cccccattgc
     2461 gagggaagcc gtggtgctcg ccgtgggcgc gagtcccagg ggacaggc
//
LOCUS       DASSLD010000396         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1209176, whole genome shotgun sequence.
ACCESSION   DASSLD010000396 DASSLD010000000
VERSION     DASSLD010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209176"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..345
                     /locus_tag="VFK23_07210"
     CDS             160..345
                     /locus_tag="VFK23_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318903.1"
                     /translation="MDKDKIGDLFSKMSPASKKELLNDLVASLLKDVSPTDKKQVLQS
                     ILAGRQGSGQVIDMVER"
     gene            370..642
                     /locus_tag="VFK23_07215"
     CDS             370..642
                     /locus_tag="VFK23_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318904.1"
                     /translation="MNPVEMSKEVISAISHSYELATYSTPELRGLFNDWLGEIEREIL
                     EFLSDRNRADPDEVAGHFKLERESAIFILSKLAREGKINIQASGSE"
     gene            1016..2179
                     /locus_tag="VFK23_07220"
     CDS             1016..2179
                     /locus_tag="VFK23_07220"
                     /inference="COORDINATES: protein motif:HMM:NF040157.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1988..1990,aa:Sec)
                     /transl_table=11
                     /product="DUF5714 domain-containing protein"
                     /protein_id="HET7318905.1"
                     /translation="MKIIEDLARTTTSRDPFEIAELMMSYPGLPMLGCQHAYIAGGAF
                     LAAVKNEGSRGVTDSDLAEVFGRTEKQAHGGYCGLSGVCGIAPAIGACFSVLTGSKCG
                     TDKEQRTTMEAVTRVSRAITDLTGPSCCKAYVRSALDVAAAYLKDSLGIILPSHKKIT
                     CSFTNKHPHGCREMDCPYFAGTARTHSAETGTNPAIISSRIVQALNRNSGEDTMEQLL
                     KRALELGAENAKIISTHTVVVEEWVRWKCRYGCAFYEKDGCHPPVAPDAESTRKVMRE
                     YSKAVLLRGPKGKQLTEIAVRLEGEAYHSGFYKAFALTALASGGSGASUPSGEKETPG
                     EPQNAEVAKMRVRPLMEACGIDVFKTARANGFDIDTKKDTYGGWNYFALVLLE"
     gene            2231..>2683
                     /locus_tag="VFK23_07225"
     CDS             2231..>2683
                     /locus_tag="VFK23_07225"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HET7318906.1"
                     /translation="MPVKTSGLITGFLACLFLLSHASVIHAANDTRFTIKDGVIDDSE
                     TGLQWVPAGNWEMTHYEAEKYVSSLGLAGGGWRLPTRAELRSIYDASKPGLADPKFKI
                     SGKLVWTSELDTDPSLAWYFNFYSGGEYTYTRNPHCNFYFYALAVRSRR"
BASE COUNT          637 a          698 c          818 g          530 t
ORIGIN      
        1 cggttcttcg gtggcagggg cggattttgc cgcaggccgc cggcggaccg cgacagcgac
       61 atcgatgaat tgagaaaaga gatcaaagcc ctgagggatg aggtgaggga gttgaagaac
      121 atgacgattc tcaaatgaag acttcccgga ggcggcataa tggacaaaga taaaattgga
      181 gatctgtttt cgaagatgag tcccgcatcg aagaaggaac tgctgaacga tcttgtggcc
      241 tccctgctca aggacgtcag cccgacagac aaaaagcagg tgcttcagtc catcctcgcg
      301 ggaaggcaag gctccggcca ggtcatcgac atggtggagc gctgaataca ggaatgcacg
      361 gattccggtg tgaatcctgt ggagatgagc aaagaggtca tttcggcgat cagccattcc
      421 tatgagctgg ccacctattc aacccccgaa ctgagggggc tattcaacga ctggctcggc
      481 gaaatcgagc gggagatcct ggaatttctg tcggaccgga acagggcgga ccctgacgag
      541 gttgccggtc atttcaagct ggagcgcgag agcgccatct tcatcctgag caaactggcc
      601 cgggaaggga aaatcaatat ccaggcatcg ggaagcgagt aagagcatcg ggaagaccgg
      661 cccctggagt ctcaggttcc tgtacggaac ggcggcgttg agcccgggtc tggtgttgta
      721 taccctgctg cacagataga ggaattgtac aatgacggac gatatcaaaa agaaaaaaag
      781 cgcctcttca tgctgaccct ccgaaactga tgccggtcgg cagcagagtg tcagtactac
      841 ggccccggtg ccggaaaacc gcagcaccga gcactgcatg gtctgcagtt cttccctgtc
      901 gtaccttgac cgggctcggg acgtgacctg ctcctattgc gggaaggtcg agcagggcca
      961 tatcaggtgt cccaacggcc actacatctg cgacgcctgc cacaaccggg ataccatgaa
     1021 gatcatcgaa gacttggccc gcacgacgac gtcgagagac ccctttgaga tcgcggaact
     1081 gatgatgagc tacccgggtc tccccatgct tggttgccag cacgcctaca ttgcgggcgg
     1141 ggctttcctg gccgccgtga agaacgaagg ctcgcgaggc gtcacggaca gcgaccttgc
     1201 cgaagtcttc gggcgcacag agaagcaagc ccatggcggc tactgcggcc tttccggagt
     1261 atgcggcatc gctcccgcca tcggagcctg cttttccgtc ctcaccggtt caaagtgcgg
     1321 gaccgacaag gaacagcgga ccacgatgga ggcggtaacg cgggtgtcgc gggcgataac
     1381 cgatttgacc ggcccgagct gctgcaaggc ctatgtccgg agcgccctgg acgttgccgc
     1441 ggcctatctt aaagatagcc tcggaatcat tctcccttct cacaaaaaaa taacatgctc
     1501 cttcacgaat aaacatcccc acggctgcag agaaatggat tgcccgtact ttgcgggcac
     1561 tgccaggacg catagcgccg agacgggcac gaacccggca atcatctcat cacgcattgt
     1621 tcaggcgctg aaccggaact ccggggagga tactatggaa cagctgttaa agcgggccct
     1681 tgaactgggc gcagagaatg cgaagatcat ctccacgcac acggttgtgg tagaggagtg
     1741 ggtcaggtgg aagtgcaggt acggatgcgc gttctacgag aaggacgggt gccatccgcc
     1801 cgtcgcacct gatgcggaaa gcactcggaa agtaatgagg gaatacagca aggccgtgct
     1861 cctcagggga ccaaagggga agcaactgac cgaaattgcg gtccggcttg agggagaagc
     1921 gtatcacagc ggtttttata aggctttcgc tttgacggcg ctcgcttccg gcggcagcgg
     1981 ggcgtcctga ccgtctggtg aaaaggagac tccgggcgag cctcagaatg cggaagtggc
     2041 caagatgagg gtgcggcccc tcatggaagc ctgcggcatc gatgttttca agacggcgcg
     2101 ggctaacggc tttgacatcg acacaaaaaa ggatacgtac ggtggctgga attactttgc
     2161 gcttgtactt ctggagtgag gtaaccgcta gtagccaatt caaaaacatc aaattcatag
     2221 gagcccttct atgcctgtaa agacgagtgg cttgatcacg gggtttttgg cgtgcctgtt
     2281 tctgttgtca catgcgtcgg tcatccatgc cgctaatgat acgagattca ctatcaagga
     2341 tggggttatt gacgattcag aaaccgggct ccaatgggtg ccggctggta attgggagat
     2401 gactcattat gaggcagaga agtatgtcag tagtttaggc ctggccggag gcggatggcg
     2461 tcttccgaca agggcggaac tgaggagcat atacgatgca tccaagccag gtcttgctga
     2521 ccccaagttt aagattagcg gtaagcttgt atggacatca gagctggata cagatccatc
     2581 gttggcgtgg tacttcaatt tctacagcgg tggtgagtac acgtacaccc gcaaccccca
     2641 ctgcaacttc tatttttacg ctctggcggt gcggtcccgg cgg
//
LOCUS       DASSLD010000397         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_261540,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000397 DASSLD010000000
VERSION     DASSLD010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261540"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFK23_07230"
     CDS             complement(<1..320)
                     /locus_tag="VFK23_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318907.1"
                     /translation="MSGSDGGKKARAASASGEELAALLHDPSPEVIKAILSNSNLSDD
                     DVLVIVNRKNVPAHLLDMIARDERWKDSYPVRLALAKNPKTPLSVSLSIVRYLHLFDI
                     AEMTR"
     gene            445..981
                     /locus_tag="VFK23_07235"
     CDS             445..981
                     /locus_tag="VFK23_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET7318908.1"
                     /translation="MERTLLLNATFEPLKIISWQRAITLLTQGKVEVIDFYDRDIRGV
                     SITFRLPSILRLLKLVKVRRASREVRFSRANIYQRDKYTCQYCGVRYPAEDLTFDHVI
                     PQVQGGQATWENIVTACLSCNNRKGGRTPQEAKMRLIRPPHKPRWMPVVTMTLGMKNA
                     PLSWRDYLYWNIELTRDD"
     gene            1178..1810
                     /locus_tag="VFK23_07240"
     CDS             1178..1810
                     /locus_tag="VFK23_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318909.1"
                     /translation="MQNKILLCIIFGAVMVSAAGQAVAAPRFTQNEFKTAGSLDPGMT
                     QTGINFSLSDDFKSYYADIRYGLGAMFELGGKFGVAVTTLDATDKMGALVGVDVKYQL
                     IKETEGIPLDLAVDVSFDNTIVSSKNASELTFSSILSKDFFLTDRGYKLVPYGGLSLS
                     SLYGSLTEQRETFVNLFAGLEWKISQKAMFLVEIKGGDRLVGGGSIRFEY"
BASE COUNT          463 a          586 c          605 g          448 t
ORIGIN      
        1 cgggtcatct cggcgatgtc aaagaggtgg agataccgca cgatggaaag ggagaccgaa
       61 aggggcgtct tcgggttctt ggccagcgcg aggcggacag gatagctgtc cttccagcgc
      121 tcgtcccggg cgatcatatc gagcaggtgg gcaggcacgt tcttcctgtt cacgatcacc
      181 aggacatcgt cgtcgctcag gttgctgttt gacagaattg ccttaatgac ctcgggagat
      241 gggtcgtgga gcagtgcggc aagctcttcg ccgcttgcgg aggccgccct ggcctttttc
      301 cccccgtcgg aaccgctcat gaccacatta tacgggtttt caaacctatt gacaaacacc
      361 aaagttttct gtatttttat acctcgacaa ctggaatttt cgtgcttttc ggttcttctt
      421 tatcacggca gtacgagggt tgcaatggaa aggacgcttc tcctcaacgc gacattcgaa
      481 ccgttaaaga tcatctcatg gcaaagggcc atcaccctcc tgacccaggg caaggtcgag
      541 gtaatcgact tctatgaccg cgatattcgc ggagtgtcca tcaccttcag gcttccctcc
      601 atcctcaggc tgctgaaact cgtcaaggtg cggcgcgcca gcagggaagt gcgtttttca
      661 cgggcaaata tctaccagcg ggacaagtac acctgccagt attgcggggt caggtatcca
      721 gccgaagacc tgaccttcga tcatgtcata ccgcaggtcc agggcggcca ggccacctgg
      781 gagaacatcg tgactgcatg cctgtcctgc aacaacagga agggcggcag gacgccccag
      841 gaggcgaaga tgcggttgat ccgtcccccg cacaagcccc ggtggatgcc ggttgtcacc
      901 atgaccctcg gcatgaagaa cgcccccttg agctggcgcg attacctcta ctggaacatc
      961 gaactcacga gagacgactg aacgaggcgg ggaagaaatc acaggccccg gccgttcatc
     1021 ggataatccc gaccgcgatc tgctcatgcg ggagaatttc tttcccccct ttgcccgctt
     1081 ccggcatgtc ggattttttt tcttgtcaac aatggtgttt gatgctatat tccgaatcgc
     1141 cgcacgaatg atcctggcgg cacggaggca atgaggaatg caaaataaaa ttttattatg
     1201 catcatcttc ggcgcagtca tggtgtcggc agcgggccag gcggttgcag ctccccgttt
     1261 tacgcagaat gaattcaaga ccgccgggag cctggacccc ggcatgacgc agaccggcat
     1321 caatttcagc ctcagcgatg atttcaagag ctactatgcg gacatccgtt acggcctcgg
     1381 tgcgatgttt gagctggggg gcaagttcgg cgtcgcggta acgaccctcg atgcgaccga
     1441 caaaatgggg gcgttggttg gcgtcgatgt caagtaccag ctcatcaaag agaccgaggg
     1501 gataccgctc gatctcgcgg tggacgtgtc gttcgacaac acgatcgtca gcagcaaaaa
     1561 cgcaagcgaa ctgacctttt catctatcct cagcaaggac tttttcctca cggaccgcgg
     1621 ctacaagctc gtcccttacg gcggactgtc gctgtcgtcc ctttacggat cgctcacgga
     1681 acagcgagag acattcgtga atcttttcgc ggggctcgaa tggaaaatca gccagaaggc
     1741 catgttcctt gtggagatca agggcggcga ccggctggtg ggcggaggga gcatcaggtt
     1801 cgagtattga cgggcttccg gccacgcgga gccgtcgcag gtatgtctat tcctctttca
     1861 gggaccattc ggacgaaacc gctttcagca atccttgagc atttgcggcg gcgaacggca
     1921 acgggcgtgc tcacgctgcg tgcgggtgac gtcgctaaaa gcatctacgt caaggacggc
     1981 aagatcatct tcgcgacatc aacggagccg tctgaccgcc tgggcgagat tctcgtcaag
     2041 tcgggaaaaa tcacgaggga aaatctggaa caggcccttc gggtctacca gaagagcgca
     2101 gg
//
LOCUS       DASSLD010000398         3437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1206437, whole genome shotgun sequence.
ACCESSION   DASSLD010000398 DASSLD010000000
VERSION     DASSLD010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206437"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2266
                     /locus_tag="VFK23_07245"
     CDS             <1..2266
                     /locus_tag="VFK23_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7318910.1"
                     /translation="DGASVFLLDRSGKTVTLASAVGLHQEQMGKISFPLGKGVAGWVA
                     EQKVPLALEDPYSDPRFEYVPESGIGKFKSLVAMPILDEDRCLGVIFVLSVQSWRATS
                     AELTLLSTTANQISGVIKSTQLIQSIQDRLSELATIYEIGMALTSTLDLEQLLTLIAK
                     NSAQSLRAQGCTIRLLNLPEIASHKTFASYSVAGGSITSVDQRIDLAVTDRVERDKQP
                     LLIQDTRDDASLRECPADSACSVISVPLLYRDKLIGIITVFNKKEGQPFTEDDLHFLT
                     TVASGAAVAVENAAMYERMEDLADEARARAQELAILNDIGTAMSTTLNLDRLLRIILT
                     AATMGGSGLGFNRAVLLLTNERTNILQGMMGVGPTNWEEAGKAWSEVSSRHKGLMEWI
                     QTGDLFEHRDSKFNDLARGIRTVLDPHEGVLALTVLGKRSYNITDASSEPIVPKELRE
                     LLQVNSFATVPLVAKDRVIGVILVDNMFTQRPITDRDVRFLTMFAHQSALAIENALIH
                     TNLETMNRDMRALHEQLVQSEKMAALGAMMAEITHEVRNPLVSIGGFTRRLAKKLQQG
                     EEKKYIDIILSEVSRLEGIIHDNLSFIRESAPQLTLSDMNTLLREVFLLYEDELANRR
                     IRVVQDLSAALPALLIDQQQIKQAVINILKNAMEAMENGGTLTVRTYLTDDVREAAVE
                     FGDTGPGISSKVMHNIFNPYYTTKPRGTGLGLPITNRIVKAHKGKIELRNKDGGGAVF
                     TIKLPCPKTEEKKA"
     gene            2421..2771
                     /locus_tag="VFK23_07250"
     CDS             2421..2771
                     /locus_tag="VFK23_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7318911.1"
                     /translation="MKKILVVDDEANIRELYKEEFEEMGFAVTAVADGIQALGAMDAD
                     KFDLVTLDMRMPDMDGIETLRKMKEKDSTLPVVICTAYEDYKHDFGSWCSDAYIVKSA
                     DTDLLRETVKKILG"
     gene            2768..>3437
                     /locus_tag="VFK23_07255"
     CDS             2768..>3437
                     /locus_tag="VFK23_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HET7318912.1"
                     /translation="MKERAEVIVEKLVHGGSGLARLESGQAVFVSGVLPGERVAVQIR
                     RKRKGFLEADLIEVIVPSQDRIEPLCRGEKQCTGATWPHIAYPAQLRYKEDILLDALA
                     RTGGMRPRRPLPILPSHRTDHYRLRTQFNVRSKEGTQRIGFFRQGSYDLVEVDDACLI
                     DPAISRTLKAIRALVHLLPPLTEIHVNATPSGQVHLLVFSDQNSLPSVEPFYARLTET
                     APAVI"
BASE COUNT          753 a         1074 c         1021 g          589 t
ORIGIN      
        1 ggacggtgcg tccgtattcc tcctcgaccg ctcggggaaa accgtcaccc tcgcatcggc
       61 ggtcgggctg catcaggagc agatgggaaa gataagcttt ccgctgggga agggcgtcgc
      121 gggatgggtt gcggagcaga aggtgccgct cgccctcgaa gatccctact ccgacccgcg
      181 cttcgagtac gtcccggagt cgggtatcgg aaaattcaag tccctcgtgg ccatgcccat
      241 cctggatgag gaccgctgcc tgggcgtgat cttcgttctt tccgtccaga gctggcgggc
      301 cacgtcggcg gaactcacgc ttctttcgac aacggcgaac cagatcagcg gcgtgatcaa
      361 gagcacccag ctgatccaga gcatccagga ccgcctgtcc gagctcgcaa cgatctacga
      421 gatcggcatg gcactcacct ccacgctcga cctggagcag ctcctgacgc tcatcgccaa
      481 gaacagcgcg cagtccctcc gcgcccaggg ctgcaccata cgactcttga accttcccga
      541 gatcgcctcc cataagacct ttgcctccta cagcgtcgcc ggaggctcca tcacgagcgt
      601 cgaccagagg atcgatcttg ccgtaacgga ccgggtcgaa cgggacaagc agccgctcct
      661 gatccaggac acccgcgacg atgcctccct ccgggagtgc ccggcagaca gcgcctgctc
      721 cgttatcagc gttcccctgc tctatcgaga caagctgatc ggcatcatca cggtctttaa
      781 caagaaggaa gggcagccgt ttaccgagga cgacctgcat ttcctaacca ccgtggcgag
      841 cggcgccgcc gtggccgtcg agaacgcagc aatgtacgaa cgcatggaag acctcgccga
      901 tgaggcgcgg gcgcgggcac aggaactcgc gatcctcaat gacatcggca ctgccatgag
      961 tacgactctg aacctggacc gcctgctccg gatcatcctg acggccgcca ccatgggggg
     1021 ctccgggctt ggcttcaacc gggccgtcct ccttctcacg aacgagcgga ccaacatcct
     1081 gcagggcatg atgggggtcg gcccgacgaa ctgggaagag gcgggcaagg cctggagcga
     1141 ggtatcgagc agacacaagg ggctgatgga gtggatccag acgggggacc tcttcgagca
     1201 ccgcgattcg aagttcaacg acctcgcgcg cggcatcaga accgtcctcg atccgcacga
     1261 aggggtcctc gccctcacgg tgctcggcaa acgttcctac aacatcaccg acgcttcgtc
     1321 ggaacccatc gtccccaagg aactccggga actgctccag gtgaacagct tcgcaaccgt
     1381 gccgctcgtg gcgaaggacc gagtcatcgg cgttatcctc gtcgacaaca tgttcaccca
     1441 gcggcccatc accgaccggg atgtccggtt cctcaccatg tttgcgcacc agtccgccct
     1501 ggccattgaa aacgccctca ttcacacgaa ccttgaaacg atgaacaggg acatgcgggc
     1561 gttgcacgag cagctcgtac agtccgaaaa gatggcggcc ctcggcgcca tgatggcgga
     1621 gatcactcac gaagtcagga acccgctcgt gtccatcggc gggttcacgc ggcggctcgc
     1681 gaagaaactg cagcagggcg aggagaagaa gtacatcgat atcatcctga gcgaggtctc
     1741 gcgcctcgag gggatcatcc acgacaacct gtcctttatc agggagtcgg ccccgcagct
     1801 caccctgtcc gacatgaaca ccctgctccg ggaggtattc ctgctctatg aagacgagct
     1861 ggccaatcgc cgcatccggg tggtgcagga cctgtccgcg gccctccccg ctctcctgat
     1921 cgaccagcag cagatcaagc aggccgtcat caacatcttg aagaacgcca tggaggccat
     1981 ggaaaacgga ggcacgctga cggtcaggac ctatctcacg gacgacgtgc gggaggcggc
     2041 cgtggaattt ggggacacgg gtccgggtat ttcgtcgaag gtcatgcaca acatcttcaa
     2101 tccctactac accaccaaac cgcgcggcac ggggctcggg ctgccgatca ccaacaggat
     2161 cgtcaaggcc cacaagggga agatagaact gcggaacaag gacggcgggg gggctgtttt
     2221 cacgatcaaa ctgccctgcc cgaaaaccga ggaaaagaag gcttgaacgt cgtatgatgt
     2281 ggtaatatca gagaggatag atcggctgtt ggaccttcga cttaggtcat acaggcatcg
     2341 ccgatatcct tccgcttatc atcgcggtga agaggatcgg cgcaggaaat tcagaagctc
     2401 gttgggtaaa ggaggcattg atgaaaaaga tactggttgt cgatgacgag gcgaatatcc
     2461 gggagctcta taaggaggaa ttcgaggaaa tggggttcgc cgtgaccgcc gtcgccgacg
     2521 gcatacaggc gctcggggcg atggacgccg acaagttcga cctcgtcacg ctcgacatgc
     2581 gcatgcccga catggacggg atcgagacgc tgcgcaagat gaaggaaaag gacagcaccc
     2641 tgccggtcgt catctgcacg gcctacgagg attacaaaca cgacttcggg agctggtgtt
     2701 ccgatgccta tatcgtgaaa tcggcggaca cggacctgtt gcgggaaacg gtcaagaaaa
     2761 tcctgggatg aaggagcgcg cggaggtcat cgtcgaaaaa ctcgtgcacg gcggaagcgg
     2821 cctggcgcgg ctggaaagcg gccaggccgt gttcgtgtcc ggggtgctcc ccggagagcg
     2881 cgtcgcagtc cagatccggc ggaaacggaa agggtttctg gaggccgacc tgatcgaggt
     2941 cattgtcccc tcacaagacc gcatcgaacc attgtgccgc ggtgaaaaac agtgcaccgg
     3001 cgcaacgtgg ccgcacatcg cctatccggc ccaactccgc tacaaagagg acatccttct
     3061 tgatgcgctt gcacgcacgg gcggcatgag gccccggagg cccctcccca tcctcccttc
     3121 gcaccggacc gatcattacc gcttgcgtac gcagttcaac gtccgctcaa aagagggaac
     3181 ccagcggata ggatttttcc ggcagggatc gtacgacctc gttgaagtgg acgatgcctg
     3241 tctcatcgat cccgcgatca gcaggacgct gaaagccatc cgagcactcg ttcacctgct
     3301 tccaccgctc accgagatcc acgtcaatgc aacgccttcc ggacaggtgc acctcctcgt
     3361 gttcagcgac cagaactcat tgccctccgt ggaaccgttt tatgcgcgcc ttacggaaac
     3421 agcgcccgcg gtcatcg
//
LOCUS       DASSLD010000399         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_753525,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000399 DASSLD010000000
VERSION     DASSLD010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753525"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VFK23_07260"
     CDS             complement(<1..276)
                     /locus_tag="VFK23_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318913.1"
                     /translation="MNNKIQSRSVFLLVVFAAIIGIPVIAGAQEQDIPKKFFVPPPPF
                     SPGIFPCSQCHQGMPVNRRPRKLEQMHQDITLKHMPGGWCFDCHNPDN"
     gene            complement(330..1223)
                     /locus_tag="VFK23_07265"
     CDS             complement(330..1223)
                     /locus_tag="VFK23_07265"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET7318914.1"
                     /translation="MRNVIIVVAATILFMLPAAGHAGYKQDFENEFLTKTWAGVQMEE
                     NLCISCHASEKMKPEFREITEAWQASWHAQNNISCENCHGGDPRDAALAMSPERGFIG
                     RPDYKDVPDFCGKCHIAILANFRDSGHGKAHAAGGTSPNCMTAIKAGKKAPNCVTCHG
                     SHNIQKASIDIINEQLCTQCHSYERAKIMKQALFQTENRIEQLEKKLQVLRQGGIYPE
                     TDEKSLFNTQAEYRALFHTVDVNLVKNRTDEFTAKLDQIDKNIQAAYQELRFRKNFSA
                     LLMLSFSCMGIVIFLISRRND"
     gene            complement(1223..>2036)
                     /locus_tag="VFK23_07270"
     CDS             complement(1223..>2036)
                     /locus_tag="VFK23_07270"
                     /inference="COORDINATES: protein motif:HMM:NF025016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HET7318915.1"
                     /translation="LMIVVVLLHMVTVFLMGNYKRPRELTWLGGGLALIVTMTFGYSG
                     HLLPWSQMSYWSTTVVTSMPSAFPVVGDTIANILRGGDHITGTTLSRFFELHIAILPL
                     CFLTLMVIHLFLVYRTGISATPFGKQDTEERPLTEYKRKAYPDGYAYYPVFFQKQMYM
                     MMAYLAVMFFIITFLPTLFFPPEANIAADPFKTPHDIRPAWYLLAQYEILRLIPNKFF
                     GILLQIILAVIFLFWPFLDTQREKNLMKRPVLRGVFVFLLASWVFLLFLGRS"
BASE COUNT          462 a          529 c          574 g          471 t
ORIGIN      
        1 gttgtccgga ttatgacaat caaagcacca gccgcccggc atgtgtttca gagtgatgtc
       61 ctggtgcatc tgctccagct ttcgcggccg tctattgacc ggcatgcctt gatggcactg
      121 ggaacaggga aagataccgg gcgaaaaagg gggcggggga acaaagaatt tttttgggat
      181 gtcctgctct tgagcgccgg cgatcaccgg aatcccgatg atcgccgcga acacaacgag
      241 caggaacacg cttcttgact gtattttatt attcatctca tctccccttt tcatgtgggg
      301 tgggtgtccg gcgaacggag gcgctgctct taatcgttcc tccgcgatat gaggaatatg
      361 acgatgccca tgcaggaaaa ggacagcata aggagagcgg agaaattctt tctgaatctc
      421 agctcctgat aggcggcctg gatgttcttg tcgatctggt ccaactttgc ggtaaattca
      481 tccgttctgt tcttgacgag gttgacatcc acggtgtgga agagcgcgcg atattccgcc
      541 tgagtgttga acaggctctt ttcgtccgtt tccgggtaga ttcctccctg tctcagcacc
      601 tggagtttct tttcaagctg ttcaatccta ttttccgtct ggaaaagggc ctgtttcatg
      661 attttcgccc gttcatacga atgacactgc gtacagagct gttcattaat gatatcgatg
      721 cttgccttct ggatgttgtg cgacccgtga caagtcacgc aattcggggc ttttttaccg
      781 gccttgatgg ccgtcatgca attgggtgac gttccccccg ccgcgtgcgc cttgccgtgg
      841 ccgctgtcgc gaaagttggc aaggatagcg atatggcatt tcccgcagaa atccggcacg
      901 tctttgtaat ccggacggcc gatgaatcca cgctcgggcg acatggcaag cgcggcgtct
      961 ctcggatcgc cgccgtgaca gttctcgcag gagatattgt tctgcgcatg ccagctcgcc
     1021 tgccacgcct cggtgatctc ccggaattcc ggtttcatct tttccgatgc gtgacaggag
     1081 atgcagaggt tctcctccat ctgcaccccg gcccaggtct tcgtaagaaa ttcattttcg
     1141 aagtcctgtt tatagcccgc atgacccgct gcgggaagca tgaagagtat cgtcgccgca
     1201 acaacgatga tgacgtttct cattacgacc tccccaaaaa cagcagaaac acccacgagg
     1261 cgagcagaaa tacgaatacg ccccgcagga ctggccgttt catgagattc ttttctcgtt
     1321 gggtatccag aaaaggccaa aagagaaaga tcacggccag gatgatctgg agcaggatgc
     1381 cgaaaaactt gttcggaatg agcctgagga tctcatactg tgcgagcaga taccacgccg
     1441 gcctgatgtc gtgcggcgtt ttgaacggat cggcggcgat attggcctca ggggggaaga
     1501 agagcgtcgg cagaaacgtg atgatgaaga acatgaccgc aagatacgcc atcatcatgt
     1561 acatctgttt ttggaaaaag acgggatagt acgcgtatcc gtccggatag gccttccgtt
     1621 tgtattccgt gagggggcgc tcttccgtat cctgcttgcc gaacggggtc gccgagatgc
     1681 cggtgcgata gacgaggaac aggtggataa ccatcagggt caggaagcac agcggcagaa
     1741 tggcgatatg cagttcgaag aacctgctga gcgtcgtacc cgttatatga tcaccgcccc
     1801 tcaggatatt cgcgatagta tcgccgacga cgggaaaagc cgaaggcatg gaggtgacca
     1861 cggtcgtcga ccagtagctc atctgcgacc atggaagaag gtgaccgctg taaccgaagg
     1921 tcatcgtaac gatgagcgca agccctcccc cgagccaggt aagctcacgg ggacgtttgt
     1981 agttgcccat gaggaacacc gtcaccatat gcaggagaac gaccacgatc atgagg
//
LOCUS       DASSLD010000400         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_992690,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000400 DASSLD010000000
VERSION     DASSLD010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992690"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /gene="topA"
                     /locus_tag="VFK23_07275"
     CDS             complement(<1..1383)
                     /gene="topA"
                     /locus_tag="VFK23_07275"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET7318916.1"
                     /translation="MKSLVIVESPAKAKTISKYLGRNYTVKASVGHIMDLPKSKLGVD
                     IENNFEPKYIMIKGKAPVVKELKSAAKKADRILIATDPDREGEAIAFHVAETISDSSK
                     TQKIYRVLFNEITKKAILQAIEHPGKIDNNKVDAQQARRVLDRLVGYQISPILWQKVR
                     RGLSAGRVQSVALRLICEREDEIKAFVPEEFWSLTALLEGKLPPQFEAKLVKKDEEKL
                     KVKSNEEVQKILADLQGKTYVVSAVEKKERRRNPVPPFTTSKLQQEAGRKLGFTAKRT
                     MGIAQSLYEGVEVGKEGAVGLITYMRTDSTRVGKEAQDEARDFIADKYGKDYLPAKPP
                     VYASAKAAQEAHEAIRPTSVLREPDAIRQYLEVDQYKLYKLIWNRFVASQMNPAIIDQ
                     TSVDISANGYTFRATGSIVKFPGFMAVYLEEKSEDQAPEEDSDEAVLPPLVEGEKLAL
                     IKLDPKQHFTQ"
     gene            complement(1490..1963)
                     /locus_tag="VFK23_07280"
     CDS             complement(1490..1963)
                     /locus_tag="VFK23_07280"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HET7318917.1"
                     /translation="MDDRMMTLFSLIADQVREKKELFDEEGKIMEALLNNGCRLHEAD
                     AALTLAQTMVQRHSEDFFPAAANTSPGMRTMNREERNRFTVEAFGFVTKLTNLGVIGE
                     DQREELLERAMAAYPERIELDQIKSLVALSLFVHPLEQDVNVPSAGRRIRKTSWN"
     gene            complement(2049..>3060)
                     /gene="dprA"
                     /locus_tag="VFK23_07285"
     CDS             complement(2049..>3060)
                     /gene="dprA"
                     /locus_tag="VFK23_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET7318918.1"
                     /translation="AERALAASPGELRDIEGLSCKVVEAIGAFEWQKSAEREVAKAEE
                     TGVAIITLDEPSYPKRLRDTPDPPLYLYIRGRLAPEDDNAVAIVGTRRPTHYGKTVTQ
                     RLARELASSGFSVVSGLARGIDTEAHLGALAADGRTIAVLGCGVDVAYPPENRGLIDR
                     ISRSGRGAVITENPFGTKPEAGYFPARNRIISGLSRGTVIIEASEDSGSLITAQYARE
                     QDRKLFAVPGNIASPVSRGAHSLIKQGATLVEDVKDILAGLGESTGYAHRKTVARPMP
                     QLSPGETAVLQSLSGEPKHIDALLQESRMNAGALSGALITLELKGLAKQLPGKYFIRT
                     EI"
BASE COUNT          493 a          924 c          925 g          718 t
ORIGIN      
        1 ctgggtgaag tgctgcttcg ggtcgagttt gatgagtgca agcttctcgc cttcgacgag
       61 tggcggcagg accgcttcat cgctgtcctc ttccggggcc tggtcttcgg acttctcctc
      121 caggtagacg gccatgaagc ccgggaattt cacgatcgat ccggttgccc ggaacgtata
      181 tccattggcg ctgatgtcga cagaggtctg gtcgatgatc gcggggttca tctggctcgc
      241 gacgaaccgg ttccagatga gcttgtagag cttgtactgg tcgacctcga ggtactgtct
      301 gatcgcgtcg ggctcccgca ggaccgacgt ggggcgaatc gcctcgtgcg cctcctgcgc
      361 ggccttggcg ctggcataga ccggaggctt tgccgggagg tagtctttcc cgtatttgtc
      421 tgcgatgaaa tcgcgcgcct cgtcctgcgc ctccttgccc acgcgggtgg agtcggtcct
      481 catgtaggtg atgaggccga cggcgccctc cttgcccact tccacgccct cgtagaggga
      541 ctgggcgatg cccatggtcc tcttggccgt aaatccgagc ttgcggccgg cctcctgctg
      601 cagtttgctc gtcgtgaagg gagggacggg atttcgccgt cgttccttct tctccaccgc
      661 ggagacgacg taggtcttcc cctggagatc ggccaggatc ttctgcactt cttcgttgct
      721 tttgaccttg agcttttcct cgtccttctt gacgagcttt gcctcgaact gcgggggaag
      781 cttcccctcc aggagcgcgg tcaaagacca gaactcctcg gggacgaagg ccttgatctc
      841 gtcctcgcgc tcgcatatca ggcggagcgc gacggactgt acccggccgg ccgaaagccc
      901 ccggcgcacc ttttgccaga ggatggggct gatctgatag cccacgaggc ggtcaaggac
      961 gcgtcgcgcc tgctgggcgt caaccttgtt attatcgatc ttgccggggt gttcgatggc
     1021 ctgaagaatg gccttcttgg tgatctcatt gaagaggacg cggtaaatct tctgcgtttt
     1081 ggagctgtct gaaatggttt ccgccacgtg gaaggcgatc gcttcgccct cgcggtcggg
     1141 gtcggtcgcg atcaggatgc ggtcggcttt tttggcggcg gacttgagct ctttcacaac
     1201 cggggccttg cccttgatca tgatgtactt cggctcgaaa ttattttcga tatccacgcc
     1261 cagcttgctt ttcggcaggt ccatgatatg tcccaccgag gccttgacgg tgtagttcct
     1321 gccgaggtat ttggagatgg tcttggcctt ggcgggtgat tcgacaatga cgagtgattt
     1381 catgtccgtt taaaaccttt ttgccgcaga gcacgacgaa gacacagagg aatctcttaa
     1441 aaaaatattt ctccgtgctc tctgtgcgct ctgtggcaaa tcttgaattt caattccagg
     1501 aagttttcct gatgcgcctg ccggccgaag ggacattcac gtcctgctct agagggtgga
     1561 cgaacaggga caaggcaacg aggctcttga tctggtcgag ttcgatccgt tccgggtatg
     1621 cggccatggc ccgttccagc agttcctccc gctggtcttc gccgatgacg ccgaggttcg
     1681 tcagtttggt tacaaagccg aaagcttcaa cggtgaaccg gttgcgctct tcccggttca
     1741 tggtcctcat gcccggggag gtgtttgcag cggccgggaa aaaatcctcc gaatgtctct
     1801 gtaccatcgt ctgggcgagg gtcagcgcgg cgtccgcctc gtgcaatcgg cacccgttgt
     1861 tgaggagagc ctccatgatc ttgccttcct catcgaagag ctcctttttt tcccgaacct
     1921 ggtccgcgat gagcgagaac agggtcatca ttctatcgtc cacgtttttc tccttggaaa
     1981 ccgtaccgca aagaatcagc tcgttgcaga tggcccggcg tatccgtttt tccccgatga
     2041 gctacgggct aaatttcagt ccgtataaaa tacttccctg ggagctgttt tgcaagtcct
     2101 ttcagctcca gggtgatcag tgccccgctc agggcgccgg cgttcatacg gctctcctgc
     2161 aggagagcat cgatgtgctt cggttcgccc gaaagactct gcagcacggc ggtctcaccc
     2221 ggagagagct gcggcatggg ccgcgccacc gtcttccggt gcgcgtagcc ggtactctca
     2281 ccgaggccgg ccagaatgtc tttcacgtcc tcgaccaggg ttgcgccttg cttgatcagg
     2341 ctgtgcgcgc ccctgctgac cggagaggcg atgttccccg gcacggcaaa aagctttctg
     2401 tcctgctcac gggcatactg cgcggtaatg agcgagccgc tgtcctccga tgcctcgatg
     2461 atcacggtgc cgcgcgagag gccgctgatg atcctgttcc gtgcgggaaa atacccggcc
     2521 tccggtttcg tgccgaaggg gttttccgtt atgaccgcgc cgcgtccgga ccggctgatc
     2581 cggtcgataa ggcccctgtt ctcgggagga tacgccacat cgacgccgca tccgagcacc
     2641 gcaatcgtcc tgccatcggc cgcaagtgca cccaggtggg cctcggtgtc gatgccccgc
     2701 gccaggccgc ttaccacgct gaagcccgaa gaggcgagtt cgcgggcaag ccgttgcgtg
     2761 acggtctttc cgtagtgcgt gggtctgcgt gttcccacga tcgccacggc gttgtcatcc
     2821 tccggggcca gcctgcccct gatgtacagg tagagcggcg gatcgggcgt gtcccggagt
     2881 cttttgggat aggagggttc gtccagcgtg atgatcgcga cgcccgtttc ctccgccttc
     2941 gcgacttccc gttccgcaga cttttgccac tcgaaggccc cgatcgcttc gacgaccttg
     3001 cacgaaagcc cctcgatgtc ccgcagctcg ccgggcgacg cggcgagcgc ccgctcggcc
//
LOCUS       DASSLD010000401         2746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_166797,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000401 DASSLD010000000
VERSION     DASSLD010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166797"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1635
                     /locus_tag="VFK23_07290"
     CDS             97..1635
                     /locus_tag="VFK23_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007695485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HET7318919.1"
                     /translation="MSSEIRESTPPSPREVRTVFAGLMLALALASLDQNIVGIALPRI
                     VSDLGGLHHLSWVVTSFLVTSTATTPLYGKLSDMYGRKPLFIVAIVIFLIGSCLCGLS
                     RSMTELVVYRGVQGLGAGGLMVLAQTTIADLVAPRDRGRYQGLFGAVFAFSSIAGPLL
                     GGFITSALSWRWIFYVNVPVGIPALAMIVFGFHRPHHKVTHHIDYSAVVLLTTATVAL
                     LLVLSCGGVQYAWSSPLIIGLAMAAALLTVLLIRTERRSEEPVLSPHIFHNRTVVVAS
                     GVMGLAFMGIFGAFVFMPLFFQLVLGMTPAKAGLLLAPMTGGMIMTSFLGGRIVSRTG
                     RYKTLTVSGLGGATIAFIAVMIAAAVGGGIGVIETALIVLGCGFGMVMPNLVVSIQNA
                     VGPREQGAATATAAFFRQLGGTFGVAMSGAIMTAVLRGLEEEKSTGLVRGGRALLEQG
                     VQQIAGLPPTQRELVIGAYRHAISMTFLMGSIIMALAFFLVLYLPEHPLRSAHIAEGE
                     KNQG"
     gene            1636..2625
                     /locus_tag="VFK23_07295"
     CDS             1636..2625
                     /locus_tag="VFK23_07295"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HET7318920.1"
                     /translation="MALAAHGRDAGSITDFGREENIHKVLFTFSLIAAAGYIWRFFDF
                     GGLDRHRVRHALSTAVFHVFLPALAFGLIAGATVDWSFIAVPLTAAAATLACLAVGYG
                     IYHFLPQLRQIPRPALGVLLLSSAFGNVTYLGLPVINETLGPQYGYVAILYDLLATTP
                     ILLTLGVLIAARTGSGKAVSLRASLKRVFLLPPLWGVALGMAVHLLPVKVPALILDAA
                     ALMGRAVIPVMIFTVGLALDFRDFGRLPLALPALAVKLFLAPVLTWWAGSFLGVSGSV
                     LQALALEGAMPVMVLSLVIADEFCLDVPLAATCIAVSTLASLVTAPLIFRLPV"
BASE COUNT          401 a          895 c          819 g          631 t
ORIGIN      
        1 taggtgaagt tctaagcaaa cagagcactt gatttaatct gcgaaaatcg gcgttcatct
       61 gcgtccaact gccgttttcg gaataatcct acgactatgt cttccgaaat tcgagaatca
      121 actccgccct cgccccgcga agtcaggacc gtcttcgccg gcctcatgct ggcgctcgcc
      181 ctggcctcac tggaccagaa catcgtgggc atcgcgctgc cgcggatcgt gagcgacctg
      241 gggggccttc atcacctctc gtgggtcgtg acctctttcc tggtgacctc gaccgcgaca
      301 acaccgctgt acgggaagct ctcggacatg tacggcagga agccgctctt catcgttgcc
      361 atcgttatct ttctcatcgg ctcctgcctc tgcggcctgt cacgctccat gaccgagctc
      421 gttgtgtatc gcggcgttca gggcctgggc gccggcggcc tcatggttct cgcgcagacc
      481 acgatcgccg acctcgtggc cccgcgggac cggggccgct accaggggct tttcggcgcg
      541 gtctttgcgt tctccagcat tgccgggccg cttctgggcg ggttcatcac gagcgctctg
      601 tcgtggcgct ggatctttta cgtgaacgtg cccgtcggca ttccggccct ggccatgatc
      661 gtcttcggct tccaccgtcc ccatcacaaa gttacgcacc atatcgatta ctcggcggtc
      721 gtgctcctga ccacggccac tgtggccctg ctcctggtgc tctcctgtgg cggcgttcag
      781 tacgcatggt cgtcgccgct cattatcggg ctggccatgg ccgccgctct gctcaccgtc
      841 ctgctgatcc gtacggagag gcggtcggag gagccggtgc tctcgccgca catcttccac
      901 aacaggaccg tggtcgtcgc gtccggcgtg atggggctcg ctttcatggg gattttcggc
      961 gccttcgtgt tcatgcccct tttcttccaa ctcgtcctcg gcatgacgcc tgcgaaagcg
     1021 ggactcctgc tcgctcccat gaccggaggc atgatcatga cgtccttcct cggagggcgc
     1081 atcgtgtcca ggaccggacg atacaagacc ctgacggtct ctggcctggg cggggcaacg
     1141 attgccttca tcgccgtgat gattgcggcc gccgtgggtg gcggtatcgg cgtgatcgag
     1201 acggcgctca tcgttctcgg ctgcggcttc ggcatggtca tgcccaacct ggtcgtatcg
     1261 atccagaacg ccgtcggccc ccgcgagcag ggagcggcaa cggcgaccgc cgcttttttc
     1321 cggcagctcg gcggcacgtt cggcgttgcc atgtccggcg ccatcatgac cgccgtcctc
     1381 aggggccttg aggaggagaa gtcgaccggc ctcgtccggg gaggccgcgc cctcctggag
     1441 cagggcgtcc agcagattgc cgggcttcct ccaacccagc gcgagctcgt catcggcgcc
     1501 taccggcatg ccatcagcat gacctttctt atgggctcga tcatcatggc tctggccttt
     1561 ttcctggtgc tctatctccc ggagcatccg ctcaggtcgg cgcatattgc cgagggagaa
     1621 aagaaccagg gctgattggc ccttgcagcg catggccgcg acgcaggttc tataacggat
     1681 tttggcaggg aggagaatat tcacaaggtc ctattcacat tcagcctgat cgcagcggcg
     1741 ggatatatct ggaggttctt cgacttcggg ggtcttgacc gtcaccgtgt gcgtcatgcc
     1801 ctgagcacgg ccgtcttcca tgtctttctg cccgctcttg cctttgggtt gatcgccggg
     1861 gcgaccgtcg actggagctt tatcgccgtc cccctcaccg ccgcggcagc aactctcgcc
     1921 tgtcttgcgg tcggatacgg catctaccat ttccttccgc agctccgaca aatccccagg
     1981 ccggccctgg gcgtccttct gctttcctcg gccttcggca acgtcaccta tctggggctt
     2041 ccggtcatca acgaaacgct cgggccgcag tacggatacg tggcgatcct ctacgatctt
     2101 cttgccacga cgccgatcct cctgaccctg ggcgtgttga ttgccgcccg gaccggaagc
     2161 gggaaggcag tttctctccg agcctccctg aagagggtct tcctgctccc cccgctctgg
     2221 ggcgtcgcct tgggcatggc ggtacatctg ctgccggtaa aagttccggc gcttattctc
     2281 gatgcagcgg cgctcatggg cagggcagtg ataccggtca tgatctttac ggtaggtctg
     2341 gcgttagatt tccgggactt cggaaggttg cccctggccc tgccggccct ggcagtcaag
     2401 ctgtttctgg cccctgttct tacatggtgg gccggctctt tcctcggggt ctcgggcagc
     2461 gtcctccagg cgctggcgct cgagggcgcc atgccggtga tggtgctttc cctggttatc
     2521 gcagatgaat tctgcctgga cgttccgctg gcggccacct gtatcgccgt ctccaccctg
     2581 gcgtctctcg tcacggcgcc cctcatattc cgcctgccgg tatagtcttc tgaaaatgct
     2641 gtcaacatac tgagcgcggt ttgtctgtcc cggaagggtt atggcaggca atcgcagcgg
     2701 tttatatcgt caaaaagggc ggagttcccg gggctgctcc aggagg
//
LOCUS       DASSLD010000402         4647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_969338,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000402 DASSLD010000000
VERSION     DASSLD010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969338"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..493
                     /locus_tag="VFK23_07300"
     CDS             38..493
                     /locus_tag="VFK23_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET7318921.1"
                     /translation="MERNNRKGEGLGLNLIPMMDVLTVLVLFLLFHSFNGEMPEQRIE
                     LPDSVVDTRPRETLAIVVNPEAVLIQGAAVVSTSELYEDRIGMVVQIKERLEGLEREI
                     GITAPESKEITLLADKTIPFKVLKKIMSTCTASGYGRISLAVVQKVAKI"
     gene            516..1970
                     /locus_tag="VFK23_07305"
     CDS             516..1970
                     /locus_tag="VFK23_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET7318922.1"
                     /translation="MNTSTLAEELEQLNLQIEQARKKHDVLVSELRPVEAELESFSAE
                     RQRFDALRDACNAFDKLKEMKADELFWGGISEAQNNAGSMERARGQIASFEGEIGGIL
                     EKQASLKQQINQCLDELDILEDEVRNAYDREERRLEEYIIEREISSVPYREMIMPWTR
                     ETESEKRFRRYASIALLLCFFLSTSIALIKLPVLKPPPLVVVPERLVSLLKKEPPKPV
                     KVRKPVPKQVREAPGQAKKEPKPRPDQVVPEKTSEPAVAGGGSPAPRKKAETAGVLAF
                     KEAFRDLMKETPVARLGTEARLSNEDPRVKGGLAQRSLVSMVGGSSGGIGGAGVSRNV
                     GSGKGGRGGGYGGYGVGSGSGGGSGDGVGFARVGSPIAGLGESSRPTSDGLALGRTDE
                     EIQIVFDRYKASLYRIYNQELRKDPTLRGKILMRLSIEPDGAVSLCKVESTELASPEL
                     VDKIVDRIRRFNFGPKEGVQKVTILYPIDFLPAG"
     gene            1994..3301
                     /locus_tag="VFK23_07310"
     CDS             1994..3301
                     /locus_tag="VFK23_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7318923.1"
                     /translation="MISDDYAITELMEKLRERGFADVTRRKIRFFVETGLIPGPSGKG
                     GGARYGEDHLLRILAINKLRKTFWKIADIKSHLKNMSPEDIRQFTLTPEPVIPAPNAE
                     GTDGKLRRGLLVRSSTFESFTRKDASTTSGSHPSGLMSADTWHRLNVMEGMELNMRSD
                     ALRRLMKEPPDGKREILASLEMITLGRTSRAVSSRESGIPSSLSERFECDLINGRLLA
                     VIGGQRALLDTGFPFSFGQCGSITLCGREFAISKGAYPTTEELSKLIETEFSLVLGAD
                     VLSRLDFRIDLPELEITFSENFGAIRGSAVPLRIISGLPVLTARLPNGEFPFLISTGM
                     KLSFLDSNIASTFPVVGKGSDAIPRIGKFEGTKFETDLRRIKVRLEEKNLKLSFGVLP
                     DVIEDSTLKGFGVMGIVMGILGAELFANNPACVSYRRQRLTFL"
     gene            3396..3472
                     /locus_tag="VFK23_07315"
     tRNA            3396..3472
                     /locus_tag="VFK23_07315"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3430..3432,aa:Pro,seq:tgg)
     gene            3958..4614
                     /locus_tag="VFK23_07320"
     CDS             3958..4614
                     /locus_tag="VFK23_07320"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HET7318924.1"
                     /translation="MQYRVDTVPLLLRPVFYLYGYGLGFFLYAFYAIQRLTIGVQVEG
                     QDNLVAGFNYIFCHWHESVGLSLQCSVPRLPSFLRDRPHVWMQHPAWYMKPLHVVLGL
                     IGVKKLVLGSSGHNGRKAADELVGYLQSGYSTVLLPDGPYGPPKVLKKGILHIAHQSH
                     VAIVPLQFSASRFLVIKTWDCKQIPMPCSRVRIHIGRPITVSEHTFDHAEQKLCRDLG
                     "
BASE COUNT         1092 a         1165 c         1378 g         1012 t
ORIGIN      
        1 gagacgcacc atgaatgaat ccagacgaat gagacggatg gagcgcaaca acagaaaagg
       61 tgaaggtctg ggtctgaacc tgataccgat gatggatgtg cttactgttc tggtgctttt
      121 cctgcttttt cattcattca atggtgaaat gccagagcag aggattgagc ttcccgattc
      181 agtcgtcgat accaggccaa gggaaacgct ggccattgtt gtcaaccccg aagcagtatt
      241 gatacagggg gcagccgtgg tcagtacttc cgaactttat gaggacagga tcgggatggt
      301 tgttcaaata aaagagcggc tggaggggct tgaacgggaa atcggcataa cggctcccga
      361 aagcaaggaa atcactcttc tggccgataa aacgattccg ttcaaagtgc tgaaaaaaat
      421 aatgtcaacc tgcacggctt ctggatacgg aaggatttcc cttgccgttg tccagaaggt
      481 cgcaaaaatc tagtggggct gccgtggggg ctgccgtgaa tacatctacg ttagctgagg
      541 aattagaaca gctgaacctg cagatcgagc aagcacggaa aaaacatgac gtgctggtaa
      601 gtgagctgcg acctgtagag gcggagctgg aatctttttc cgccgaacga cagcggttcg
      661 atgccttgcg ggacgcctgc aatgcgttcg acaagctgaa agaaatgaaa gcggatgaac
      721 tcttttgggg aggaatttcg gaggcacaaa ataacgccgg atctatggaa cgggcgaggg
      781 gccagattgc cagcttcgag ggggagatcg gcgggatcct ggaaaaacag gcatctctca
      841 agcagcagat aaatcaatgc ctggatgaac tcgatatcct ggaagatgaa gttcgtaatg
      901 catatgaccg tgaagaaaga cgcctggaag agtacatcat agagcgggag atttcgtccg
      961 taccttatcg cgaaatgatc atgccctgga ccagggagac agagagcgag aaacgcttcc
     1021 gtcgatatgc ttccatagcg cttttgctgt gctttttcct tagcacttcg atcgctctga
     1081 taaaacttcc ggtcctgaag cctccgcccc tggttgtcgt tccggaacgc ttggtctctt
     1141 tactgaaaaa ggaaccgcct aagcccgtaa aggtccggaa gccggtgcca aaacaggtcc
     1201 gggaggcgcc tggccaggcg aagaaagaac ccaaaccccg gcccgatcaa gttgtaccgg
     1261 aaaaaacgtc ggaaccggcc gttgccggcg gcgggtctcc ggcgccgcgg aaaaaagccg
     1321 aaacggcagg tgttctggcg tttaaagagg ccttcaggga cctgatgaaa gaaactcccg
     1381 ttgcgagact cggcaccgag gcccgcctga gcaatgagga cccgcgggtg aaaggggggc
     1441 tggcgcagcg ttcgctggtg tccatggtgg gcggctccag tggaggtatc ggcggcgccg
     1501 gcgtcagccg caacgtagga agcgggaagg ggggccgggg cggcggctac gggggctacg
     1561 gggttggcag cgggtctggc ggcgggtctg gcgacggcgt gggctttgct cgggtaggaa
     1621 gccccatagc gggtttggga gaatcgtcga ggccgacgag cgatggactt gctttgggcc
     1681 ggactgacga agaaatccag attgtattcg atcggtacaa ggcgtcgctt tatcggatct
     1741 acaaccagga actgcgcaag gacccaacgc tgcgcgggaa gatattgatg cgactcagca
     1801 tcgaaccgga cggcgcggtg tccttgtgca aggtggagtc caccgagctg gcttcgcccg
     1861 agctggtaga caagattgta gatcgcatca gaaggttcaa ctttggcccg aaagaaggcg
     1921 tgcagaaggt gaccattctg tacccaatcg acttcttgcc cgccggatag aagagcgaaa
     1981 gggtcaggac gggatgattt cagatgatta cgccataacc gagcttatgg agaagttgcg
     2041 tgagcgcgga ttcgctgatg ttacaaggcg taaaatccga ttctttgtcg aaacagggct
     2101 gatccctgga cccagcggca aagggggcgg cgccaggtac ggagaagatc acctgcttcg
     2161 catcctggcg atcaacaagc tgcgaaagac attctggaag atcgccgaca tcaagagcca
     2221 tctgaaaaac atgtcccccg aggacatccg ccagttcacc ttgaccccgg aaccggtgat
     2281 tcccgctcca aacgcagagg ggacggacgg gaagcttcgc cgcggattac ttgttcgaag
     2341 ttcaaccttc gagtcattca cgcgaaagga cgcatcaacc accagcggat cacacccttc
     2401 cggactcatg tcggccgata cgtggcatcg tctcaacgtg atggaaggga tggaactcaa
     2461 tatgcggtcc gacgcactcc gacgtctcat gaaagagcca cccgacggga aacgcgagat
     2521 tctcgcttct cttgaaatga tcacgctggg gagaacttcc cgcgcggtct ccagccgcga
     2581 atcgggaatc ccctcctccc tttcggaacg attcgagtgt gatctgatca acggccgcct
     2641 tctcgccgtg atcgggggcc aacgcgcact gctggacacc ggcttcccct tcagcttcgg
     2701 gcagtgcggc tcaatcacac tctgcggtag ggagttcgcg atctcaaagg gggcataccc
     2761 gacgacagaa gagttgagca aactcattga gaccgaattt tccctggttc tcggggcgga
     2821 cgtactcagt cgattggact tccggatcga cttgcctgag ctggagatca cgttttcgga
     2881 aaactttggg gccatccgtg ggagcgcagt acctctccga atcatcagcg ggctaccggt
     2941 tctcacggcc cgtcttccaa atggagagtt cccattcctg atctcgacgg ggatgaagct
     3001 ttcattcctc gattcgaaca tcgcgagtac gttccctgtt gttgggaaag gttcggacgc
     3061 catcccgagg ataggaaaat tcgaggggac gaagtttgag acggacctcc ggcggatcaa
     3121 ggtgaggctc gaggaaaaga atctgaagct atccttcggt gtgttgccgg atgttatcga
     3181 ggattcaacc ctgaagggtt ttggcgtgat gggtatcgtg atgggtatat tgggagcgga
     3241 gttgttcgcc aataatcctg cctgcgtctc ctaccggcgt cagaggctga ccttcttata
     3301 gccagataat tgggagcagg tcgctgcgac acgactcgtc aggtcgattt actggcgggg
     3361 cgtttccgtt tatactccga gggtctatca agagtcggga cgtggcacag cctggtagcg
     3421 catctgcttt gggagcagag ggtcgctggt tcgaatccag tcgtcccgac catgaaatca
     3481 aggggttacg gattcaaggt ccgtagccct ttcttatttt gcgaccgttt tgcgaccatc
     3541 tttttctccg gcctcccaat ccggcaagct ttcgaccgct tttctcgtgt gctcgggggt
     3601 gaggtgagat atgtatgaca gaaaagatac accagccgaa gggcgcccat cccggactgc
     3661 tgaatataga agaggaggcg tagtgatcgg gagtcgcaaa ttatgtatca acttgggtgg
     3721 gatcgtgatg gcgatagctg caagtgaggt cctctctcgt tgatgtcggt cattatttga
     3781 caagccccga atgaaagaat aaattcatct ttcagcatcg atattctctt cgagacaatc
     3841 gcagaaccgt cccggcaagc agggaggcgt gatgcgggct ctcgaccgtt cccaccaaca
     3901 gaaaaaaccg tctcccgacc cagtgcggaa gatttgggtt tttcagaggg cgccagaatg
     3961 caataccgag tcgatactgt tccacttttg cttcggcccg tgttctatct gtacggatac
     4021 ggccttggat ttttcctgta tgcgttttat gcgatacagc gattgaccat cggcgttcag
     4081 gtcgaggggc aggacaattt ggtggctggc ttcaactaca tcttctgcca ttggcacgaa
     4141 tccgttggtc tgtcgctgca atgcagcgtt ccccggttgc cgtcatttct acgcgatcgg
     4201 ccgcatgtct ggatgcagca cccggcgtgg tacatgaagc cgctccacgt agtattgggt
     4261 ctcattgggg tgaaaaaact ggttcttggg tcaagcggac ataacggacg taaggccgca
     4321 gacgaacttg tagggtatct ccagagtggc tactccacag tgctgctgcc cgatggtccg
     4381 tatggacctc ccaaggtatt gaaaaaaggg attcttcata tcgcacacca gagccacgtc
     4441 gcgatagttc ccttgcagtt ctctgcctca cgctttctgg taatcaagac gtgggattgt
     4501 aagcagatcc cgatgccatg ctcaagggtt cgaattcata taggacgacc gatcactgtc
     4561 agcgaacata cgttcgacca tgctgagcaa aaactttgca gggatcttgg atgaatgatt
     4621 tatttaagcg gtctaaccac aggctga
//
LOCUS       DASSLD010000403         3144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_99878,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000403 DASSLD010000000
VERSION     DASSLD010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99878"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..311)
                     /locus_tag="VFK23_07325"
     CDS             complement(60..311)
                     /locus_tag="VFK23_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318925.1"
                     /translation="MAQSTTKKKPVGAMIVMGIISIALYATLLAKQDLVNSTFAKGGL
                     YALLPIITAFVFSYFHGSFTGHFWTVLGVEASKKKKEVK"
     gene            complement(494..994)
                     /locus_tag="VFK23_07330"
     CDS             complement(494..994)
                     /locus_tag="VFK23_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7318926.1"
                     /translation="METTKLKKIMKSVEHALTAASFAEEGDPETANSLMKESRRVLLA
                     LKAGLIDQKTLKYALNTAKRINAQLDILHVVSSRGGQGGDPALESFLSELSSEGVAYR
                     LIIRSGCLKQQIIDYTNSEKEILFAVIESPSSLDADCDRQDKELSELWRKLKCPLVVV
                     MEGAKA"
     gene            complement(1087..1263)
                     /locus_tag="VFK23_07335"
     CDS             complement(1087..1263)
                     /locus_tag="VFK23_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318927.1"
                     /translation="MKGIFKKFETAMVAAAFAEEGEFETARQIMNEDRPRKTDRPSAY
                     DRQRPSNRKELRAD"
     gene            1565..>3144
                     /locus_tag="VFK23_07340"
     CDS             1565..>3144
                     /locus_tag="VFK23_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7318928.1"
                     /translation="MKFFKKSEDDHEESITGELRQKISAARMPDGVRKIAEQELDVIT
                     RINPSSAEYTIGITYLDYLLSLPWNRRTEDNLDLGRAERILNENHYGLGTIKERVLEH
                     LAVKVLMMSKKARVLIVDDEEIARRNLVHVLAKEEYDVVAAADGGSALRELEAAEFDV
                     VLTDLRMGGIDGMDLLDRIKVRHPETRVIMVTGYATVPSAIEAMQKGAFHYIAKPIKL
                     DEVRSAIKLALEKKTVRANTKGSVLCFAGPPGTGKTSLGRSIARALGRKFTRISLGGL
                     KDEADIRGHRRTYVGAKPGRVIEEIRRVESANPVIMLDELDKIGRDFKGDPASALLEV
                     LDPEQNRAFVDHYLDAPFDLSSVMFILTANMLDTVPAPLRDRMEVIEFSGYTEEEKAH
                     IASLFLCPKQIREKGLSPDTVSFTDEAIVKIIREYTREAGIRNLERKIATVCRKIAKA
                     SVELPGGPLPVLVTADAVERYLGRCRYHADVIGEQDRVGVATGLVRTESGGDIIFVEA
                     AKMKGTKELIITGSLGDV"
BASE COUNT          693 a          900 c          848 g          703 t
ORIGIN      
        1 acaggtagag gatcgtaatc gaatcaagat tgatgaaatt cgtcgcaatg tcgtgcatct
       61 tattttacct ccttcttctt cttcgaagcc tctacaccga gcacggtcca gaaatgaccg
      121 gtgaagctgc cgtggaaata ggaaaacacg aaggccgtga tgattggcag cagtgcatac
      181 aatccgcctt tcgcaaaggt gctgttcacc agatcctgct tcgccaggag ggtcgcatag
      241 agcgctatgg aaatgatgcc cataacgatc atcgcgccta ccggcttctt ctttgtggtg
      301 ctttgtgcca tgataattcc tccttagaga tattttacaa atgcaaaagc aaatcttttt
      361 taccgcagag gacgcagagg tcgctgagtt tgaatcgtaa gggtaaattt tgaatcctga
      421 accgtcatca tggtttccga ccctggtttt ctctgcgtac tctgcgtcct ctgcggtaaa
      481 tgttcttttg aacctaagcc ttcgccccct ccattaccac gacgaggggg cacttcagtt
      541 tccgccataa ttccgaaagt tccttgtcct gcctgtcgca gtcggcatcg aggcttgacg
      601 gagactcgat gacggcgaac agtatttctt tttcactgtt cgtatagtca atgatctgtt
      661 gtttgagaca accggaccgg ataatcagcc ggtaggcgac gccttcggac gagagttccg
      721 acaggaacga ctcgagtgct gggtcgcccc cctgcccgcc gcgcgaggag acgacatgga
      781 gtatatcgag ctgagcgttg atcctcttgg ccgtgttcag ggcgtacttc agtgttttct
      841 ggtcgatcag ccccgccttg agcgcaagca gcacgcgccg cgattccttc atgagcgagt
      901 tcgcagtttc cgggtcccct tcttcagcga aggaggccgc cgtcagcgcg tgctccacgg
      961 acttcataat cttcttgagc ttcgtcgttt ccatggtccc tcttgagaaa aaacagcccg
     1021 tgactctccg aattctgctg ccttctccac tctgaacttt gaactctgta ccctgaatcc
     1081 ggcagttcag tccgcccgca gttcttttct gttggacggt ctctgtcgat cgtaagccga
     1141 cggccggtcc gtttttctcg gtctgtcttc gttcatgatc tggcgggcgg tctcaaattc
     1201 gccctcttcc gcgaaagccg ccgccaccat tgctgtctca aatttcttga aaatgccttt
     1261 catggttggt ccctcctttc ccttcgatct actattctct attacaacct tcatgccaaa
     1321 tcgaagaggt ccggcattcc attgaaatta cggtataatt tcctagacca tctttttgaa
     1381 gccgtagaga gattgtttta gattgcaata cctttgccca acaaacccat tacctattga
     1441 atttaaaacg ttttattggt tgttgcaata gaattgaaat cgttgtaaaa tgcaaccatt
     1501 gcaaatttgt ctcttagaca tgaactaaag gcacacattt ccggtagacg gacgcaagta
     1561 cataatgaaa ttcttcaaga aatcggaaga cgatcacgag gagtccatca ccggagaact
     1621 gcgccagaaa atatcggcag cacggatgcc ggacggcgtt cggaaaatcg ccgagcagga
     1681 acttgacgtc atcacaagga tcaacccttc ctcagccgag tacaccatcg gcatcaccta
     1741 cctcgattat ctcctttccc ttccctggaa caggaggacg gaagacaatc tggacctcgg
     1801 ccgcgccgaa cggatcctga atgaaaacca ctacgggctc ggtacgatca aggagcgcgt
     1861 gctggagcac ctggccgtca aggtgctcat gatgagcaaa aaagcgcgcg tcctgatcgt
     1921 tgacgatgaa gagatcgcac gcaggaacct cgtccacgtt cttgcgaagg aagagtacga
     1981 cgttgtcgcc gccgctgacg gcgggagcgc gctcagggaa ttggaggctg cggagttcga
     2041 cgttgttctc accgatctcc gaatgggcgg gatagacggc atggacctcc tcgaccggat
     2101 caaggtccgc catccggaga cccgcgtcat catggtgacg ggctatgcca cggtgccctc
     2161 ggccatcgag gccatgcaga agggcgcttt ccattacatc gcaaaaccta tcaagctgga
     2221 cgaggtgcgg tccgcaatca agctggccct tgagaaaaag acggtccggg cgaacacgaa
     2281 aggctcggtc ctgtgtttcg ccggcccccc ggggacgggc aagacttccc tgggccggtc
     2341 cattgcacgt gccctcggcc gaaagttcac tcgcatcagc ctgggcggac tcaaggacga
     2401 ggccgatatc cgcggacacc ggcgcaccta tgtgggcgcg aaaccgggca gggtgatcga
     2461 ggaaatccgt cgggtggagt cggcgaatcc cgtcatcatg ctggacgaac tggacaagat
     2521 cggtcgggac ttcaagggcg accccgcgtc ggccctgctc gaggtgctcg accccgagca
     2581 gaaccgcgcc ttcgttgacc actacctgga cgcccccttc gacctctcga gcgtcatgtt
     2641 catccttacg gcgaatatgc tcgacacggt ccctgccccg ctccgcgacc gcatggaggt
     2701 catcgagttc tccggctaca ccgaggagga aaaggcgcat atcgcctccc tgttcctctg
     2761 tcccaagcag atccgggaaa agggcctgtc gccggacacg gtctccttta cggacgaagc
     2821 gatcgtcaag atcatccggg aatacacgcg ggaggcgggc atccggaacc tggagagaaa
     2881 gatcgcgacc gtctgccgga agatcgccaa ggcgtccgta gaacttcccg gcgggccgct
     2941 gccggtcctg gtgaccgcag acgccgtgga acgctatctc ggacgatgcc ggtatcacgc
     3001 ggacgtcatc ggggaacagg accgggtcgg cgtggcgacg ggcctcgttc ggaccgagag
     3061 cggcggagac atcatcttcg tcgaggccgc aaaaatgaaa ggcacgaagg agctgatcat
     3121 caccgggtcc ctcggggacg tcat
//
LOCUS       DASSLD010000404         4008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1206711, whole genome shotgun sequence.
ACCESSION   DASSLD010000404 DASSLD010000000
VERSION     DASSLD010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206711"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VFK23_07345"
     CDS             complement(<1..1026)
                     /locus_tag="VFK23_07345"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318929.1"
                     /translation="MLWGRPGYKRVIRTIVLAAIALGIAANAALSQYYLGVISGEAKK
                     IPIAVLDIYDESASSGLRSLALEVLQADLRRSQIFEVMDAKKLDLIVSGNQEPTEEQR
                     KRGGTFGLQGLVWAKIQRSGKNIVLSGKLYDAATGTRLSSKEYFGNEETFRRMVHTFA
                     DEIVFRFTGEKGISRSRIAFVSDKTRSKELYVMEYDGQNPMKVTADRSICLSPAWSPD
                     GKILAYVSYRDRNPDLFALDMETGKRWKLSGSVGLNISPAWSPNGKHIALALSRDGTA
                     EIYTMGRDGSGLERLTYGASDNVSPSWAPNGRDIVFTSGRAGTPQLYVMGADGTDVRR
                     ITFEGNYN"
     gene            complement(1027..1818)
                     /locus_tag="VFK23_07350"
     CDS             complement(1027..1818)
                     /locus_tag="VFK23_07350"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HET7318930.1"
                     /translation="MEQKIGQSSHELVLSVFLSFFLHAALIIIALILYTTVSPKVYVP
                     PVYEVKLVGQPAMTAPASAAPSAPPIAKPAPAPVEKKPEPKPKAAEVKPPVKALKKGD
                     MPELSLKKTKPSRDEDTKPAKREQVKPAAEQARPAPKETAATPNASSEGSAPATGRTA
                     GIAVNPTSGDSRQLSSYADIIRNSIERNWSPPPGAKGMRAKVSFKILRTGRLFGDVNI
                     DESSGNAYFDMAAKRAILSSSPFPSMPEEFYKQYAEFSVDVQEKN"
     gene            complement(1827..2246)
                     /locus_tag="VFK23_07355"
     CDS             complement(1827..2246)
                     /locus_tag="VFK23_07355"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET7318931.1"
                     /translation="MSFGRRERRMIRDINLVPLIDVLLVVLFFYMVISPMMSRGLDVN
                     LPKSAANTVKQEERIVLTVTRRQELFVEQERVDIARLKDVLDSIRKNKPLINVYLRAD
                     RDAPYGAVVKVMDVVKRAGIDRLGMVTEASPGGETGK"
     gene            complement(2243..2959)
                     /locus_tag="VFK23_07360"
     CDS             complement(2243..2959)
                     /locus_tag="VFK23_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET7318932.1"
                     /translation="MLAITAVPSLGTGIFGLLGETGWVGRGIVVILLGFSVVSWAMIL
                     LKYQFLRKAEKESHAFLSAFRKTKNFEDLLRNAEMTKFSPLATLFVEGYREAESIIKT
                     LPDGKVTEEERPLIAQEIERSLKITTQDEVMYLERYLPFLGTTGTVGPLLGLLATVWG
                     IMDAFYGIGLKGGGDIGALAPGLAAALINTSMGLFVAIPAVISYNYFSAKINGIATRM
                     DSFSMEFMSYLERNLLRRKS"
     gene            complement(3042..>4008)
                     /locus_tag="VFK23_07365"
     CDS             complement(3042..>4008)
                     /locus_tag="VFK23_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7318933.1"
                     /translation="RPPDAGTITAKLSRQVASSLGQEEFIRVKLGSVAGNLIATPVTR
                     GAGALMSLVRADGVIRVPAQSEGIAAGHTVPVELLRSPRDIENTLVCIGSHDNALDVL
                     ANHLKKKHPEFSLSSAHVGSMGGLLALKRGEAHLAGTHLLDEETGEYNVSYIKKILPE
                     TRTVLLNLVYRTQGFIVPRGNPKGIGGFEDLKREDVVFVNRQAGAGTRLLTDLSLRKL
                     NIDPRNVKGYDHEEFTHMAVAAAVVSGAADTGLAVLSAAQALGLDFIPVAQERYDLAV
                     PQEFYESPMMKALLGIIRGDREFREQIVAMGGYDVSEMGKVMATL"
BASE COUNT          744 a         1235 c         1149 g          880 t
ORIGIN      
        1 gttgtagttc ccttcaaagg tgatgcgtcg gacatcggtc ccgtcggctc ccatgacgta
       61 cagttgcggg gtccctgccc tgcccgaggt gaagacgata tccctgccgt tcggcgccca
      121 tgaaggcgac acattatcgg acgcgccgta cgtcagcctc tcgaggccgc tgccgtccct
      181 gcccatggtg taaatctcgg cggtgccgtc ccgggagagc gccagcgcga tgtgcttgcc
      241 gttcggcgac caggcaggtg agatgttgag cccaacgctg cccgacagtt tccatcgttt
      301 gcccgtttcc atgtccagcg cgaaaagatc ggggtttctg tcgcggtacg atacataggc
      361 gagtattttc ccgtccgggc tccaggcggg agaaaggcag atggagcggt ccgccgtgac
      421 cttcatcggg ttctggccat cgtattccat gacatacagt tccttggacc gtgttttgtc
      481 cgagacgaag gcgatgcgcg agcgtgagat ccctttctcc ccggtgaacc ggaaaacgat
      541 ctcgtcggcg aaggtgtgga ccatgcggcg gaaagtctcc tcgttgccga aatactcctt
      601 gctcgaaagc cgcgttcccg tagccgcatc gtagagcttg cccgagagca cgatgttctt
      661 gccgcttctc tggatctttg cccataccag gccctggagt ccgaaggtgc ccccccgctt
      721 cctctgttct tccgtcggct cctggttccc cgagactatc aggtccagct tcttggcgtc
      781 catgacctcg aagatctggg atcgtcgcag atcggcctgc aggacctcaa gcgcgaggga
      841 acgaagtccg gatgacgcgg actcatcgta gatatcgagc acggcgatgg ggatcttctt
      901 cgcttccccg ctgatcactc cgaggtagta ctgagacagc gcggcattcg ccgcgatgcc
      961 cagtgcgatc gccgccagga ctattgttcg gatgactctc ttataaccag gccttcccca
     1021 cagcatctaa ttcttctcct gcacgtccac actgaactcc gcatactgct tgtagaactc
     1081 ttcgggcatg gacgggaaag gactcgaaga caggatggcc cgtttcgccg ccatatcgaa
     1141 atacgcattg ccgctcgact catcgatatt gacatcaccg aacaatctgc cggtccgaag
     1201 aatcttgaat gataccttcg ctctcattcc cttggcgccc ggaggcgggc tccaattgcg
     1261 ctcgatggaa ttccggatga tgtccgcgta ggatgaaagt tgtctggaat cccctgacgt
     1321 cgggttcact gcgatccccg cagtcctgcc ggtcgctggc gccgatcctt ccgacgatgc
     1381 attgggtgtg gctgccgttt ccttcggcgc aggcctggcc tgttccgccg ccggtttgac
     1441 ctgctctctc tttgccggtt tcgtgtcttc gtctctcgac ggtttcgtct tcttgagtga
     1501 caactcgggc atgtcgccct tttttaatgc cttgaccggc ggtttgactt ccgcggcctt
     1561 tggcttcggc tcgggttttt tttcaaccgg cgcgggtgcc ggcttcgcga ttggtggcgc
     1621 cgacggcgct gctgatgccg gcgcggtcat cgccggctgg cctacgagct tcacctcgta
     1681 gaccggcggc acgtacactt tcggggacac cgttgtatag agaatgagtg cgattatgat
     1741 gagggcagcg tgcaggaaaa acgacaagaa tacggaaagg accagttcat ggcttgactg
     1801 tccgatcttc tgttccaaaa aaaacctcat ttgccggtct cacctcccgg cgatgcttcc
     1861 gtcaccatcc cgagcctgtc gatgcccgcc cgcttcacga cgtccatgac cttcaccacg
     1921 gcgccgtagg gggcgtcccg gtccgcccgg agatatacgt tgatgagcgg tttatttttg
     1981 cggatggaat ccagaacgtc cttgagccgg gcgatgtcga cccgctcctg ttcgacgaac
     2041 agttcctgac ggcgagtcac ggtaagcacg atgcgttcct cctgcttgac cgtattggct
     2101 gcggacttcg gcaggttgac atcaaggccg cggctcatca tgggcgagat gaccatgtag
     2161 aagaagagca cgacaagcag cacgtcgatg agcggcacga ggttgatgtc gcggatcatc
     2221 cgccgttccc gtctcccgaa gctcatgatt tcctccggag caggttccgt tcaagatagc
     2281 tcatgaactc catggaaaag gaatccatcc gcgtggcgat gccgttgatc ttcgccgaaa
     2341 aatagttata ggaaataacg gcgggaatgg ctacgaacaa ccccatcgag gtgttgataa
     2401 gcgcggcggc gagccccggc gccagggccc cgatatcgcc gccgcccttc agcccgatcc
     2461 cgtagaaagc gtccatgatg ccccagaccg tggccaggag gcccaggagc ggacccaccg
     2521 tgccggtcgt gccgagaaac gggaggtagc gctccaggta catgacttcg tcctgcgtgg
     2581 tgatcttgag cgagcgttcg atctcctggg caatgagtgg acgctcctct tcagtcacct
     2641 tgccgtcagg cagggtcttg atgatggact cggcctcgcg gtacccctcg acgaagagcg
     2701 tggcaagagg gctgaacttt gtcatctcgg cattccgtaa aaggtcctca aagttcttgg
     2761 tcttccggaa cgccgacaaa aaggcatggg attctttctc tgcctttcgc agaaactggt
     2821 acttgagcag gatcatggcc caggacacga cggaaaatcc caaaaggatg accacgatcc
     2881 ccctgccgac ccatccggtc tcgcccaaca gaccgaatat tcccgtgccc aagctgggca
     2941 ctgcagtgat tgcgagcatg acaacctccg aagataaaat ctattcattg ctcaccgcag
     3001 aagacttccg cggtgaagag ctttgttctc gttccccgtc tttacagtgt cgccatcact
     3061 tttcccatct cgctcacatc atacccgccc atggcgacga tctgttcgcg gaactccctg
     3121 tccccgcgga tgattccgag cagggccttc atcatcggcg actcataaaa ttcctgcggg
     3181 acggcgagat cataccgctc ctgcgccacc ggaatgaaat ccaggccgag cgcctgcgcc
     3241 gccgacaata ccgcgagccc cgtgtccgcg gcgcccgaca ccacggctgc ggccacggcc
     3301 atatgggtga attcctcgtg atcataccct tttacgttcc tcgggtcaat gttcaatttc
     3361 ctgagagaga ggtcggtcaa aagccgcgtt cccgcgcccg cctgacggtt cacgaagacc
     3421 acgtcttcgc gtttcaggtc ttcaaatccc ccgatgccct tggggttgcc cctcggtacg
     3481 atgaatccct gggtgcggta aacgaggttc aagagcacgg tcctcgtttc cggcagtatt
     3541 tttttgatat agcttacgtt gtactcgccc gtctcctcat cgagcaggtg cgttccggca
     3601 agatgggcct ccccgcgttt gagcgccagg agtcctccca tgctgcccac gtgcgcggag
     3661 gagagtgaaa actcaggatg cttcttctta agatggttgg cgaggacgtc gagcgcgttg
     3721 tcatgggagc cgatgcagac cagggtgttc tcgatatcgc ggggcgaacg gagcagttca
     3781 accgggaccg tatgccctgc ggcaatgcct tcgctctgcg cgggcacgcg gatgacgccg
     3841 tcggcgcgga cgagcgacat gagagccccc gcgccccgcg ttacgggggt ggcgatcagg
     3901 ttcccggcga cgctcccgag cttcacgcgg atgaactctt cctggccgag cgacgatgcc
     3961 acctgcctgc tgagctttgc cgtgatcgtc cccgcatcgg gcggcctg
//
LOCUS       DASSLD010000405         3229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_437950,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000405 DASSLD010000000
VERSION     DASSLD010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437950"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFK23_07370"
     CDS             <1..239
                     /locus_tag="VFK23_07370"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HET7318934.1"
                     /translation="ITEWIGATTSTTTLFFGSLVFLMLMSVQFSIMISKLSGQLKDLS
                     QENALLRKQIETLARRIGMNQKGAILANEDNAGN"
     gene            448..2487
                     /locus_tag="VFK23_07375"
     CDS             448..2487
                     /locus_tag="VFK23_07375"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7318935.1"
                     /translation="MEKKLFDQPRMHALLILILGILVYSNSLQVPFVFDDAFSIVDNP
                     EIRNLRGLISDALSGQAYHPNRYVGFLTFALNYHFGGYEVTGYHLVNLLIHLTNALLV
                     YALLILTFRAPFLRNSVLQGAAGPVALLAGALFVVHPLQTQAVTYIVQRLTSLATTFY
                     LSTLVLYALARLNAGENRFFSAKGWVFYLASVATAILAMRTKEIAFTLPLLLSLYEFL
                     FFEGSLRKRSSALAPHLLTMLIIPLGMLHLGRPAGEIIGELRQATEFQPTTTRYEYLV
                     TQFRVIVTYLRLLVLPVHQNLDYDYPIYTSFFTPPVFVSFLLLATLFLLALYLHRISS
                     PIPDGSSSSLFSCRPSRAADPAVRLISFGIIWFFIALSVESSFIPITDVIFEHRVYLP
                     SVGVFTGVAVAFVMLTMKARRRGLAWVLTVIAAAAVLSLAVGTWKRNEVWKSEETLWR
                     DVVEKSPRNARAYSNLGAVLGEAGRTEEAIAALSTAIRIQPNNTKAHVNLGTALASAG
                     RREEAIRILSEAVRIEPDNANALNNLGTVLTDAGRLDEAITILNQAVRYRPENARPHY
                     NLGRAYLAAGRKIEGVASLETAVRLRPDYDDARVTLASALTRGGRFREAAILLERNMG
                     RLAGRADARYVLGIAAHCAGDSVTASRELAALRQLDTRSARQLESFLSRPCGGGQ"
     gene            2678..>3229
                     /locus_tag="VFK23_07380"
     CDS             2678..>3229
                     /locus_tag="VFK23_07380"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HET7318936.1"
                     /translation="MSMVNAMDAARDWVRDCRSLCVLSGAGVSAESGVPTFRGPQGLW
                     KQKDPMSLATPEAFEADPREVWEWYQWRRQRIRDCAPNPGHAALAAVEAAKSDFLLVT
                     QNVDGLHRVAGSRRVAEIHGSILVVRCTECGAERVERDDFAKLPPRCAACGGLLRPGV
                     VWFGEALPPGPSGAAMEMLSRCEV"
BASE COUNT          588 a          995 c          966 g          680 t
ORIGIN      
        1 ccattacgga atggattggc gccaccacgt caaccacaac gctgttcttt ggctcgctgg
       61 tatttctcat gcttatgtct gtgcagttca gcatcatgat ctcgaagctt tctggccaat
      121 tgaaggattt gagccaggaa aatgcgttat tgcgaaaaca aatcgaaacc ttggcccggc
      181 gaatcggcat gaaccagaaa ggggcgattc ttgcaaatga agacaatgca gggaattgag
      241 tcgggaggat catacggcgc ctcggacatc tgtaagatcg agccgacgcg catttcaatc
      301 ttggcgtagc atatgccttt ctggggaaca gggaggccgc cctgcgggta ctggcagtga
      361 ttaccatact cgaccagcga ctagcgcaat ctttggccag cctcctgcgc tgaaccggcc
      421 gagctttcac ggggaagccg atccatcatg gaaaagaagc tctttgatca accgcgcatg
      481 catgcgctgc tgatcctgat tctcgggatt ctcgtctatt ccaacagcct gcaggtcccc
      541 ttcgttttcg acgacgcctt ttccatcgtc gacaacccgg aaatcaggaa tcttcgcggc
      601 ttaatcagcg acgccctttc cggccaggcc tatcacccaa atcggtacgt aggttttctg
      661 acgtttgcct taaactacca cttcggcggt tacgaggtga cgggatatca tctggtcaac
      721 cttctgatcc accttacgaa cgcgttgctc gtctacgccc tgctcatcct gaccttccgt
      781 gcccccttcc tgaggaactc ggtcctgcaa ggagcggccg ggccggtggc cctgctggcc
      841 ggcgccctgt ttgtcgtcca cccgctacag acgcaggcgg taacctatat cgtacagcgc
      901 ctgacctccc tggccacgac cttttacctt tccacccttg tgctgtatgc tttggcccgc
      961 ctcaacgcag gagagaacag gttcttttca gcgaagggat gggtctttta ccttgcgtcg
     1021 gtagcaacgg cgatcctggc gatgaggacc aaggagatcg ccttcaccct gccgttactt
     1081 ctttccctct atgagttcct tttcttcgaa gggtccctcc gcaagagatc ctcggccctt
     1141 gctccccacc ttctcactat gctcatcatt cccttgggta tgctccatct cggcaggccg
     1201 gcgggagaga tcatcggcga gcttcggcag gcgacagagt tccagcccac cacaacccgg
     1261 tatgagtacc tggtcaccca gtttcgagta atcgtcacct accttcgcct gctcgtcctt
     1321 cccgtccacc agaacctgga ctatgactac ccgatctaca ccagcttctt caccccgccg
     1381 gtattcgttt ctttcctcct gctggccacg ctatttctgc tcgccctata tcttcaccgt
     1441 atcagttcgc cgattccaga cgggtcttcc tcatcgctgt tctcttgtcg cccatcacgg
     1501 gcagccgatc ccgcggtacg cctgatatcc ttcggcatta tctggttctt cattgctcta
     1561 tccgtcgaat cgagtttcat cccgatcacc gacgtcatct tcgagcaccg ggtctacctt
     1621 ccctcggtcg gagtcttcac gggggtggcc gtagccttcg tcatgctgac gatgaaggca
     1681 agaaggcggg gcctggcctg ggtcctcacg gtcatcgcgg cggcggcggt tctgtcgctg
     1741 gccgttggga cgtggaagcg caacgaggtc tggaaatccg aggagaccct ttggcgggat
     1801 gtcgtggaga aatccccgcg caatgccagg gcctatagca acctcggggc ggtgcttggg
     1861 gaggccggac ggacggagga ggcgatcgcc gcactctcta ccgcgatccg gatccagccc
     1921 aacaacacca aagcccacgt caatctgggt actgcccttg catccgccgg gaggcgggag
     1981 gaagccatac ggatcctgtc cgaagcggtc cggatcgagc cggacaatgc caacgcattg
     2041 aacaacctcg gcaccgtttt gacggacgca gggaggctgg acgaggcgat caccatcctg
     2101 aaccaggcgg tcaggtaccg gcccgaaaac gctcgtccac actacaacct gggtcgcgcc
     2161 tatctcgcgg ccggacgcaa gattgaggga gtggcttcct tggaaactgc cgtacgactc
     2221 agacccgatt atgacgatgc acgcgtcacc ctcgcgagcg ccctgaccag gggtgggcgg
     2281 ttccgtgagg ctgcgatcct tctggaacga aatatgggcc gtcttgccgg ccgggccgac
     2341 gcccgctacg ttctcggcat tgcggctcac tgcgcgggcg atagcgtaac agcaagtcgc
     2401 gaacttgccg ctctacgaca gctcgacaca cggagtgctc gacagctcga gagcttcctg
     2461 agccggcctt gcggtggcgg tcagtagact ccaggagcgg aacacccatc gaccgatatt
     2521 acgtagagag gtttctctcc ggcagggccg gactcctcgg agggcgggtt ctggtgagcg
     2581 gcgtcaacag ttacagccgg cgcttcgggg gggcaaagag tggaggcgtt cgaggtgctg
     2641 catgcgacct cccggtcttg cgcagcgtgc tcaaatcatg agtatggtca atgcgatgga
     2701 cgccgcacgg gattgggttc gtgattgccg ctccctgtgc gtgctgagtg gcgccggggt
     2761 gtcggcggag agtggtgtgc ccaccttccg cgggccgcaa gggttatgga agcagaagga
     2821 cccgatgtcg ctggccacgc ctgaggcgtt cgaggccgat ccgcgggagg tgtgggaatg
     2881 gtaccagtgg cgcaggcaaa gaatccggga ctgcgcgccg aacccgggcc acgccgccct
     2941 tgcggccgtc gaagcggcaa aatccgactt cctcctcgtc acgcagaacg tggacggcct
     3001 gcaccgggtg gccgggtcgc gtcgggtagc ggagatccac gggagcatct tggtggtgag
     3061 atgcacggag tgcggcgcgg agcgcgtgga gcgggatgat ttcgcgaaac ttcctccgcg
     3121 gtgcgccgcg tgcggcggcc tgttgcggcc cggggtggtc tggttcgggg aggcgctccc
     3181 ccccggcccg tccggcgcgg cgatggagat gctctcccgg tgcgaggtg
//
LOCUS       DASSLD010000406         4980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1127564, whole genome shotgun sequence.
ACCESSION   DASSLD010000406 DASSLD010000000
VERSION     DASSLD010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127564"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /gene="glnA"
                     /locus_tag="VFK23_07385"
     CDS             <1..814
                     /gene="glnA"
                     /locus_tag="VFK23_07385"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET7318937.1"
                     /translation="KVGIYVERQHHEVATAGQAEIDIRFSPMVKCADQLMMFKYIIKN
                     VAKRHNKTVTFMPKPLFGDNGSGMHTHQSIWKGGKPLFAGDKYAGLSQLALNYIGGIL
                     KHAKVLAAFTNPTTNSCKRLTPGFEAPVNLAYSSRNRSASIRIPMYSPNPKAKRAEVR
                     FPDPSCNPYLAFAAMLMAGLDGIENKIDPGQPLDKDIYDMAPEELANVPSMPGSLDEA
                     LDNLEADHGFLLKGDVFTKDAIEMWLSYKRSKEVDALRLRPHPYEFFLYYDI"
     gene            complement(895..2253)
                     /locus_tag="VFK23_07390"
     CDS             complement(895..2253)
                     /locus_tag="VFK23_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7318938.1"
                     /translation="MNLIVLVSLLSRLRQFDRRDRWTRRQLETYQAEALRGLREFAYA
                     RSPFYRRFHRGLENAPLHELPVLTKAMMMENFDEVVTDRAVRLADVERHVTAIRGDER
                     FLGRYWVNATSGSTGRRGLFLFNRSEWTTVLASFLRMYHRLRGPLLLPRRLKIASVVS
                     PPPWHTSRRGGETLKAWWQPTLRLHAGDPLETMVSRLNAWQPRILGAYPSILRALANE
                     QLAGRLRVSPRYLFGGAEVLTGETRRRIAEAWSGGELFDIYGATESGTIGAECNHHTG
                     YHLFEDLVIPEVVDNDNRPVPPGVYGDKLLVTVLFGRTQPLIRYEISDNVRLAGKQCP
                     CGRPFALIDGIQGRMEDILRFPAVAGGEVPIDPVVFDQVMDKLPGGEWQVVQERDGLN
                     ILLCGTPEGFSDEGLAEELRRALTAEGAVETPIRVLHVPAIPRSATGKAPLIKSNLPR
                     PS"
     gene            2627..4429
                     /gene="kdpA"
                     /locus_tag="VFK23_07395"
     CDS             2627..4429
                     /gene="kdpA"
                     /locus_tag="VFK23_07395"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HET7318939.1"
                     /translation="MTGNDILQIVLYFVILWLLAKPLGTYMARVYEGEPFWANRALGS
                     LERLFYRLCGVRPDEEMGWKKYTVSVLAFSAAGLLFLYLLQRLQAFLPLNPQKFPAVS
                     PDSSFNTAVSFVTNTNWQGYGGETTMSYLTQMAGLGVQNFVSAAAGMAVLVALIRGFK
                     RHSAETIGNFWVDLTRSTLYILMPLAIIWSLLLVSQGVVQTFSEYKTVSLTQSITYDT
                     LKLDAQGNPIKDEKGNPVTEKTEMKEQVIPLGPAASQIAIKQLGTNGGGFFNVNSAHP
                     FENPTAFSNFLEVLAILLIPAALCITFGRMIGDQRQGWALLSAMTIVLVTLMAICTWS
                     EQSGNLRFAALNVDQTASDLQPGGNMEGKDARFGIVDSAIWATATTAASNGSVNSMHD
                     SFTPMGGLYPLVLIQFGEVIFGGVGSGLYGMLVMVIITVFIAGLMVGRTPEYLGKKIE
                     MFEMKMASLAVLVVNGLVKIGTAVAVATTAGAAGVANPGPHGFSEILYAFSSAANNNG
                     SAFAGLSANTVFYNTALGIAMFLGRFWVIIPVMAIAGSLARKKLVPASLGTLPTHTPL
                     FMALLIGVIVIVGALSFMPAWALGPIAEQLTASR"
     gene            4567..>4980
                     /locus_tag="VFK23_07400"
     CDS             4567..>4980
                     /locus_tag="VFK23_07400"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733826.1"
                     /note="One of the components of the high-affinity
                     ATP-driven potassium transport (or KDP) system, which
                     catalyzes the hydrolysis of ATP coupled with the exchange
                     of hydrogen and potassium ions; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HET7318940.1"
                     /translation="MASKTKKRSLFEPTIVKKALWDSVKKLNPLYQIKNPVMFVVEVG
                     SVLTTLLCLHALFTGKGEASPGFILQISLWLWITVLFANFAESMAEGRGKAQAESLRK
                     TRREVMAKRLSAPKPDAEVTKVVSSQLKKDDIVLVE"
BASE COUNT         1034 a         1491 c         1437 g         1018 t
ORIGIN      
        1 gaaggtcggc atctacgtcg agcgccagca tcacgaggtc gcgacggcgg gccaggccga
       61 gatcgacatc cggttcagcc cgatggtcaa gtgcgccgac cagctcatga tgttcaagta
      121 catcatcaag aacgtggcca aacgccacaa caagaccgtg accttcatgc cgaagccgct
      181 tttcggcgac aacgggtccg gcatgcacac gcaccagtcg atctggaagg gcggcaaacc
      241 cctgttcgcg ggtgacaagt atgcaggcct ctcccagctt gcactcaatt acatcggcgg
      301 aatcctgaag cacgccaaag tgctcgcggc cttcacgaac cccacgacaa actcctgcaa
      361 acgcctgact cccggcttcg aagcgccggt gaacctggcc tactcgagcc gcaatcgttc
      421 ggcatccatc cgcatcccaa tgtacagccc gaaccccaag gccaagcgcg ccgaggtgcg
      481 cttccccgac ccgtcgtgca acccctacct cgcgttcgcg gccatgctca tggcgggcct
      541 ggacggcatc gagaacaaga tcgatcccgg ccagcccttg gacaaggaca tctacgatat
      601 ggctccagag gaattggcga atgtgccgtc gatgccgggc tcgctcgacg aggcgctcga
      661 caacctcgag gcggatcacg gtttcctctt gaagggcgat gtgttcacga aggacgcgat
      721 cgagatgtgg ctcagctaca agaggagcaa ggaagtggac gccctcaggc tccgtcccca
      781 tccctacgag ttcttcctgt actacgacat ctaaacaaac agggaataga cgtcaaccgg
      841 accgggaccc gaaagcgggg tcccggtttt tttttggcgc ccggcagggt gcactcacga
      901 tggacggggc aggtttgact ttatcaaggg ggccttgccc gtggcgctcc ggggtatggc
      961 aggaacgtgc agcaccctga tgggcgtttc gaccgcccct tccgccgtaa gggcccggcg
     1021 cagttcttcg gcaagccctt cgtccgaaaa gccctcaggc gtcccgcaga gcaggatgtt
     1081 caatccgtcc ctttcctgca ccacctgcca ttcgcccccg ggcagtttgt ccatgacctg
     1141 atcgaagacg accggatcga tcgggacttc tccaccggcc acagcgggaa aacggaggat
     1201 atcttccatc cgtccctgga tgccgtcgat aagggcgaag gggcgcccgc agggacattg
     1261 tttgcccgca aggcgcacgt tgtcggagat ctcgtagcga atgaggggct gcgtgcggcc
     1321 gaagagtacg gtgacgagga gcttatcgcc gtacacaccg ggcgggacgg ggcggttgtc
     1381 gttgtccacg acctcgggga tgacgaggtc ctcgaagagg tgatatccgg tgtggtggtt
     1441 gcactccgcg ccgatcgttc cgctctccgt tgccccgtag atgtcgaaca gctccccgcc
     1501 gctccacgcc tccgcgatgc ggcggcgcgt ctccccggtg agcacctcgg cgccgccaaa
     1561 aaggtagcgc ggcgataccc ggagacggcc cgcgagctgt tcgttggcga gcgcgcgcag
     1621 aatggaggga taggccccga ggatgcgcgg ctgccaggca ttgagacggc tgaccatggt
     1681 ctcgaggggg tcgcccgcgt ggagacgaag ggtgggctgc caccatgcct tgagcgtctc
     1741 tccgccgcgg cgggacgtgt gccacggagg aggtgaaacg acgctggcga ttttcagtcg
     1801 gcgcggcagg agcaagggac ctctcagcct gtggtacatg cggaggaaag atgcaaggac
     1861 cgtcgtccac tcggaccggt tgaagagaaa caatccgcgt cgccctgtgc tcccgctcgt
     1921 ggcgttcacc caataccggc cgagaaagcg ctcatctccg cggatggctg tcacatgccg
     1981 ttccacgtcc gcgagacgca cggcccggtc ggtcaccacc tcgtcgaagt tctccatcat
     2041 catcgccttc gtcagaaccg gaagctcatg gagcggagcg ttttcgagcc cccggtggaa
     2101 ccgccggtaa aaaggcgagc gcgcatacgc gaattcgcgc aggccgcgga gcgcttccgc
     2161 ctggtaggtt tcaagctgcc ggcgcgtcca ccggtcgcgc cggtcgaact gccggagtct
     2221 cgacagcagc gaaaccaaaa cgatgagatt cacccttccc tccccctcga tgccttcagg
     2281 atcgaacact gacctaagta tagttcacgg ttttccctcg gtcaattttt cgcaaaacct
     2341 cctttatgaa atcttaatgc ttttcccagg ctttttagca gcaatttaac gatcgcttgt
     2401 gctataagtt tttcaggata aaggaggcgg cgcatggata tcgtttatct cggcattatg
     2461 attgcgctct ttgttgcaag ctggtttttc gtaatgcttg ttgaaagggt gtaacgaaga
     2521 tgacaggagg agacgcaatg gaaatctttt actggattgg gggaattttg tcagcgggct
     2581 tattggtcta cctcataata gcccttctca aacctgaggc attcctatga ccggcaacga
     2641 cattctgcaa atagttctct atttcgtcat tttgtggctc cttgcaaagc cgctcggaac
     2701 atacatggcg cgggtctacg agggagaacc cttctgggcc aatcgcgcgc tcggctctct
     2761 tgagcgtctc ttctaccggt tgtgcggcgt caggcccgac gaggagatgg gctggaagaa
     2821 gtacacagtt tcagtgctcg ccttcagcgc agcgggtctg ctcttcctct atctcctgca
     2881 gcgcctgcag gccttcctgc cgctcaatcc gcagaaattt ccggctgtct ctcccgactc
     2941 ttctttcaat actgccgtca gttttgtcac gaacacgaac tggcagggat acggcggcga
     3001 gaccacgatg agttacctca cccagatggc cgggctcggg gttcagaact tcgtgtccgc
     3061 agcagccggg atggctgtgc tcgttgcact gatccgcggg ttcaagagac attcggcgga
     3121 gaccatcggc aatttctggg tcgatcttac ccggtcgacg ctctacattc tgatgcctct
     3181 tgccatcatt tggtccttgc tattggtatc ccagggcgtt gtgcagacct tcagcgaata
     3241 caaaaccgtc tcgctcacgc agtctatcac ctatgatacc ctgaagctcg acgctcaagg
     3301 aaatcccatc aaggatgaaa aaggcaatcc cgttaccgaa aagacagaga tgaaggagca
     3361 ggtcatccct ctgggccccg cagcgtccca aatcgcgatc aagcagcttg gcacgaacgg
     3421 cggcgggttc ttcaacgtga attccgccca tcccttcgag aatccgacgg cattttccaa
     3481 tttcctggag gtcctggcga tcctgctgat tccggcagcg ctctgtatta ccttcggcag
     3541 aatgatcggc gaccagcgcc agggctgggc gctcctatcg gcaatgacga tcgtgctcgt
     3601 cacgctcatg gccatttgta cctggagcga gcagagcggc aacctccggt tcgccgcctt
     3661 aaacgtcgat cagacggcaa gcgacctgca gccgggcggc aatatggagg gcaaggacgc
     3721 ccggttcggc atcgtcgact ccgcaatatg ggccacggcg accacagcgg cctcgaacgg
     3781 ctcggtcaat tccatgcacg actccttcac gcccatgggc ggtctgtacc cgctcgtcct
     3841 gatacagttt ggagaggtga tcttcggcgg tgttggctcc gggctctacg gaatgctggt
     3901 catggtcatc atcaccgtct tcatcgcagg cctcatggtc ggcagaacgc ctgaatatct
     3961 cggcaagaag atcgagatgt tcgaaatgaa aatggcctcg ctagcggtat tggtcgtgaa
     4021 cggactggtc aaaatcggaa ctgcagtggc tgtcgcgaca acggcgggtg cggcgggagt
     4081 tgcgaacccc gggccccatg ggttctccga gatcctttac gccttttcat cagccgcgaa
     4141 caacaacggg agcgcctttg cagggctgag tgcaaatacc gttttctata acacggcgct
     4201 gggcattgcc atgttcctcg gccggttctg ggtcatcatc ccggtgatgg ccattgccgg
     4261 gtcgctggcc aggaagaagc tggtcccggc aagcttgggc acgctcccga cgcacacgcc
     4321 gcttttcatg gcgctcctga ttggggtaat cgtgatcgtg ggggcgttgt cattcatgcc
     4381 ggcctgggcc ctggggccga ttgcagaaca gctgacagca tcaagataac aaacatcaaa
     4441 gtctttgcgc cacagagcac acagagaaca cggaagaaaa atgaaatttg cttttatacc
     4501 ttcaagcgat tttctctgag gtctcagtga cctctgtggc aaaagacctt tgcttcagga
     4561 gataatatgg caagtaaaac caaaaaacgt tctctcttcg aacccaccat cgtaaagaag
     4621 gccctctggg attcggtcaa gaaactcaat cccctttacc agatcaaaaa cccggtcatg
     4681 ttcgtcgtgg aggtcgggag cgtccttacg accctcctat gcctccacgc cctctttacg
     4741 gggaaaggcg aggcgtctcc gggcttcata ctgcagatat ccctctggct ctggataacc
     4801 gtgctcttcg ctaacttcgc ggaatccatg gcagagggcc gaggcaaggc ccaggccgag
     4861 tcactaagga aaacacgtcg cgaggtcatg gccaagcgtc tgtcagcgcc aaaacctgat
     4921 gccgaggtga cgaaggtcgt ctcctcgcag ctcaaaaagg acgacatcgt gctcgtggag
//
LOCUS       DASSLD010000407         4477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1142158, whole genome shotgun sequence.
ACCESSION   DASSLD010000407 DASSLD010000000
VERSION     DASSLD010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142158"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            788..1180
                     /locus_tag="VFK23_07405"
     CDS             788..1180
                     /locus_tag="VFK23_07405"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HET7318941.1"
                     /translation="MNHVMDVKIYYEDTDCGGVVYYANYLRYMERARTEYLASKGLSV
                     RKLMDEGTIFIVLRVEIDYKSPARYGDTVEIETWVRDVSRATMLFEHIMREKSSGRVF
                     VECRAKMAYVNPTGRPKRLPAEMVEKLK"
     gene            1196..1984
                     /locus_tag="VFK23_07410"
     CDS             1196..1984
                     /locus_tag="VFK23_07410"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7318942.1"
                     /translation="MESITHDSISLRGAGVVYVAQPKTGFRFTQDSILLADFCSLKSG
                     DRVLEPGAGTGVISLLLAKKFPAARFVADEVEPIAYGLLRRNIETNGLSERIVPLDRD
                     ARYLNRSFAQSSFDIIVANPPYRKAGHGRTSPSALRQSARQEPLAPLAAWLNLHKMLK
                     NGGRYFLVFPAPRAGELMAQLRRKKIEPKRLRFVHPRLHKPASLVLIEAVKAAGIGME
                     VLPPLIVHGESGGYSEEMRQVYELPQDSPSSPVHPALPSRASVL"
     gene            2029..3234
                     /locus_tag="VFK23_07415"
     CDS             2029..3234
                     /locus_tag="VFK23_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020683993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7318943.1"
                     /translation="MAEHTYTYIIIGGGLAGASAVEGIRERDKNGSILLIGAEKHLPY
                     DRPPLSKKLWFGKKKVEDIFLHDRDFYDRSGVVLAHGATVTALEGKRKSVITDAGQEY
                     RFQKLLLATGGMPRKLPIPGGNSDGICYFRTLDDYLRMRNESAEGKSAVIVGGGFIGS
                     EIAAALAVNKINVTMIFPGSSLVNRVFPEHLGRALTDQYRARGIQILANEKPSAFLRE
                     EGGFTTYTLSGKKIESDMLVVGIGISPEVGLARDSGLETGNGIVVNEYLQTSAPDIYA
                     AGDNAFFPYQALGKQTRVEHWDNALNQGKWAGRNMAGAGEPYTYMPYFFSDLFEFGYE
                     AVGEVSADLDTFADWKKENDTGVIYYMRDDKVRGAMMCNVWDKVEAARELIRKGETVT
                     EESLRGAIR"
     gene            3376..3723
                     /locus_tag="VFK23_07420"
     CDS             3376..3723
                     /locus_tag="VFK23_07420"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET7318944.1"
                     /translation="MKLAEQTAKPIAKGDVPLSRRAAGDLIRDIPQWSLTESMVTRDF
                     HFKDFHEAMDFVNGVAAIANEQDHHPDILISYNKVRLSLSTHKIGGLSMNDFIVAAKI
                     DLLDARLRSERAA"
     gene            3720..>4477
                     /locus_tag="VFK23_07425"
     CDS             3720..>4477
                     /locus_tag="VFK23_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7318945.1"
                     /translation="MNPQAVGQSAAHDALEGKVALVTGASKRIGRALSIALAEEGVHI
                     VAHDRKNLEADTLRVCGEVKDCGAKSWPVMADLEKPEEYETLVRRALQAAGSLDILIN
                     NASIFLPGTIMDIGFGDITRHMHVNAWTPLVLSREFARLARRGTIVNILDTKIAGFDR
                     EHAAYILSKHMLSVVTRLCAVEFAPEIAVNGIAPGLILPPNGKDETYLDEKAKSLPLK
                     RHGGPDDVTEAALYLLRSRFVTGQVIYVYGGRHLL"
BASE COUNT         1054 a         1258 c         1279 g          886 t
ORIGIN      
        1 aggaaaaatc ctgtccatgg gtagcgggat gaaattgtaa agacaattca agggacggtc
       61 aagataattc tgaaagccaa accgtcttta tctgtcaagg cggcaggata gcaaaaaaat
      121 aaccctgcgg caggatgaga aaagtggtat tatagttata gaaggaaatg ctctttgaca
      181 gcggtcaaat ggctttgaag gcgttgccga accatcaccg ataggtgtac agatgataac
      241 ccgtcttaaa ggggatcagg caccgcacaa gatggactgc cgaggccggt aagagggttg
      301 caagttgagc ggccaatctc ccggtttctt tacaggcatt catcaagagc gaaagagcca
      361 aaacccctaa gccgcataga cactcgggaa gagcaataaa cacgaagctt cacagggtct
      421 gcagagcgga gccgcagaag caggttgtac ttcccgggag aaaatgcgac tcagcggaat
      481 atcagaaccg gttcgcgggc agcgcctttt ttaattttcc catcaatcgc ctgctttctc
      541 cgcagtgcgc aacgctttgt cttcattcgg ctctccttcc ggatcatcca tgacgctttc
      601 gcaggcagcc ctctctatgc ccgtttattt caatttttca aagtgtttct tctgtcgtat
      661 tctttccttt tttgcccctc ttttcttctt ctcgacaaat cacgtcaaaa gccttacatt
      721 acaagccgat tgcgcataac caaccaccaa gggcacggaa taccacgcca tacaatggag
      781 agaactgatg aatcatgtca tggacgtgaa aatttattac gaggacaccg actgcggcgg
      841 ggtggtttac tacgccaact acctccggta catggagagg gctcgcaccg agtaccttgc
      901 atcaaagggg ctctccgtca ggaaactcat ggatgaaggc acgatcttca tcgtgctccg
      961 ggtggagatc gactacaagt ctcccgcgcg gtacggagat acggtcgaaa ttgagacctg
     1021 ggtgagggac gtaagccgcg cgaccatgct cttcgagcat atcatgcggg agaagtcgtc
     1081 gggcagggtc ttcgtggaat gcagggccaa aatggcgtat gtcaatccca ccggaagacc
     1141 gaagaggctg cccgccgaga tggtggaaaa actgaaataa tccgcttctg cacgcatgga
     1201 atcaataacc cacgacagca tttcgctccg cggcgcgggt gtggtctacg tcgcgcagcc
     1261 caagacaggc ttccgcttca cccaggacag catcctcctc gctgacttct gcagcctaaa
     1321 gagcggggac cgcgtgctgg agcctggcgc agggacgggc gtcatctcac tactgctcgc
     1381 aaaaaaattc ccggccgcgc gcttcgtcgc cgatgaggtc gagccgatcg cttacggcct
     1441 tctccgtcgc aatatcgaaa caaatggtct ttcagagagg atcgttcccc tcgacagaga
     1501 cgcgagatat ctgaatcgat ctttcgccca gagttccttt gatatcatcg ttgccaatcc
     1561 tccctataga aaagcgggac acggcagaac aagcccctcc gctttaaggc aaagcgcccg
     1621 gcaggagccg ctggcgccgc tcgcagcgtg gctgaatctc cataagatgc tgaagaacgg
     1681 cggacggtac tttcttgtat ttcccgcccc ccgcgcaggg gagctgatgg cgcagctcag
     1741 gaggaaaaaa atcgagccga agcgtttgcg cttcgtgcat ccccgtcttc acaagcctgc
     1801 atcgcttgtt ttgatcgaag ccgtaaaggc tgcaggcatt gggatggaag tcctgccgcc
     1861 gctcatcgtt cacggggaaa gcggaggata ctcagaagag atgcgccaag tctacgaact
     1921 gcctcaagat agcccctcat cgccggtcca ccccgccctt ccctcccggg caagtgtgct
     1981 atagtaatag tgaagaagtc gtataacccc gatgaggagg ctcttcgcat ggctgagcat
     2041 acctacacct acatcatcat cggcggcggg ctggccgggg cgtcggcagt ggaaggcatc
     2101 cgcgaacggg acaaaaacgg cagcatcctg ctcatcggcg cagagaagca cctcccctac
     2161 gaccgacctc cgctctcaaa gaaactctgg ttcggcaaaa agaaagtcga agatattttt
     2221 cttcacgacc gtgattttta cgaccgaagc ggggttgtcc tggcgcatgg cgccaccgtc
     2281 accgcgctcg aggggaaacg gaaaagtgtc atcaccgacg cgggacaaga ataccggttt
     2341 cagaaactcc tcctcgcgac gggtggaatg ccgcggaagc tgcccatccc cggagggaac
     2401 tccgacggta tctgttattt ccggacgctt gacgactacc tccggatgcg gaatgagtcg
     2461 gccgagggaa aatccgccgt gatcgtcgga ggcggattca tcggttcgga aatcgccgcc
     2521 gcgcttgccg tcaataaaat taacgtcact atgatctttc ccggttcctc cctggtgaac
     2581 cgggtctttc ccgaacatct cggcagggcg ctcacggacc agtaccgtgc ccgcggcatt
     2641 cagatcctcg ccaacgagaa gccgtcggcc tttctgaggg aagagggcgg gtttacaacg
     2701 tataccctga gcgggaagaa gatcgaatcg gacatgctgg ttgtcggcat cggcatctcc
     2761 ccggaggtcg gcctcgcgcg ggatagcgga ctggaaacgg ggaacggcat cgtcgtgaat
     2821 gaatatctcc agacctccgc ccccgatatt tacgcggccg gcgacaacgc cttctttccc
     2881 tatcaggcgc tcggaaaaca gacccgcgtc gagcactggg acaatgccct gaaccagggt
     2941 aagtgggcgg gcaggaacat ggccggagcg ggggagccct acacctacat gccgtacttc
     3001 ttctccgacc tcttcgagtt cggctacgag gccgtcggtg aggtgagcgc ggacctcgac
     3061 actttcgcag actggaagaa ggagaacgac accggcgtga tctattacat gagggacgac
     3121 aaggtccgcg gcgccatgat gtgcaatgtg tgggacaagg tagaggccgc gcgggaactg
     3181 atccgcaagg gagagacggt gacagaggag agcctgcgcg gggcgatacg gtgaaaagac
     3241 aagggaaata ttggacacgg atcaaagctg aaaaatcgga tttaacttca atgtatacag
     3301 cttggtttga agacgcgacc ggtcaatctg cgtttatctg cgcaaatccg cgtccacagt
     3361 tttttggagg acaccatgaa actcgcggaa cagacagcga aaccgatagc caagggggac
     3421 gtccctctgt cgcggagagc ggccggggac ctcatacggg atatccccca gtggtctctg
     3481 accgaatcca tggtcaccag ggattttcat ttcaaggatt tccacgaggc gatggacttc
     3541 gtgaacggcg ttgctgccat tgcgaatgag caggaccatc atcccgacat cctcatctca
     3601 tacaataagg tgaggttgtc cctgtcgacg cacaagatcg ggggcttgtc catgaacgat
     3661 ttcatcgtgg cggccaagat cgaccttctg gatgcccggc tgcgaagtga gagggctgca
     3721 tgaacccgca ggccgtcgga caaagtgcgg ctcacgatgc actggaggga aaggttgccc
     3781 tggtaaccgg cgcgagcaag cgcatcggac gcgctctttc cattgcgctc gccgaagagg
     3841 gcgtgcacat cgtggcacac gaccggaaaa acctggaggc ggacaccctc cgggtctgcg
     3901 gcgaagtgaa agattgcggc gcgaaatcgt ggcccgtcat ggcggacctg gagaaaccgg
     3961 aagaatacga aacgctcgtg aggcgcgcgc ttcaggccgc gggctcgctc gacattttga
     4021 tcaacaatgc ttcgattttt ctgcccggca cgatcatgga catcggcttc ggagacatca
     4081 cgcggcacat gcacgtgaac gcctggacgc ccctggtcct ttcccgggag ttcgcgcgac
     4141 tggcgcggcg gggaacgatc gtcaatatcc tcgacacgaa aatcgccggc tttgatcggg
     4201 agcacgcggc gtacattctg agcaagcaca tgctctcggt cgtgaccagg ctgtgcgccg
     4261 tcgaattcgc gcctgaaatc gcggtaaacg gcatcgcgcc cggcctcatc ctccctccca
     4321 acggcaaaga tgagacatat ctcgacgaga aggccaaatc ccttccgctc aagcggcatg
     4381 gcggtcctga tgacgtgacg gaggctgcgc tctacctcct ccgtagccgt ttcgtgaccg
     4441 gtcaggtcat ttacgtgtac ggcggcagac accttct
//
LOCUS       DASSLD010000408         3132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1020258, whole genome shotgun sequence.
ACCESSION   DASSLD010000408 DASSLD010000000
VERSION     DASSLD010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020258"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VFK23_07430"
     CDS             <1..893
                     /locus_tag="VFK23_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET7318946.1"
                     /translation="NTRWFQGEETEGELRGHFALQLAGLADIYVNDAFGSWQPHASTS
                     SIVSLLPSYAGFLLQQEIAHLDGVMRPERPFLAVVAGSKYDTKIRPLNAIYDKVDRLV
                     LGGVIYNTYLCAKYGVRIRGVSDEDRDLARDLVEKDRQAQKIVELPLVVESDVPDARI
                     DGKYRTISVRDFKPGGSFGYVLDIDPRSFEATAVAEAIGTAKTIFVNAVMGYTPHFTA
                     GSQALDNTIDQNRDAMKMYGGGDTLQEFKNLLPGLYLSVMDDASYYFFTGGGTVLTAI
                     EHGSPYSLEPVKALMEAKRK"
     gene            1179..2498
                     /locus_tag="VFK23_07435"
     CDS             1179..2498
                     /locus_tag="VFK23_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HET7318947.1"
                     /translation="MGKKGWLVTIAGVVMNLALGILYAWSMFSKQLTEAVEKGGFGWS
                     KTQATLPYTVAIACFAVMMVPAGRLQDRFGPRIVATIGAVLCGLGLVVASLGNPGSIL
                     PAILGFGVLAGAGIGLGYASATPAAVKWFPPEKKGLITGLVVAGFGLAPVYIAPLSKY
                     LLGTYGISSSFRILGFAFIIAAGIAAQFITNPTAPAAVNKSGPKNPPSQDFTWREMLR
                     TPAFYSLYIQYACAATAGLMIIGHLAKIVAVQSGNTIKIGFLFVALLAIFNAGGRVVA
                     GIVSDYIGRIITLVFVCIMQATVMAFFPQFSTISGFMIGSALVGFNYGACLSLFPSTT
                     ADYWGTKNLGMNYGILFTAWGVGGILGPMLAGSIADASGSYAMAYYIACGLLAFAAFF
                     GMASYISFSVSLPQKQIVVSFSGKSAKKKEAIVAPALDGGKKEEMVA"
     gene            complement(2556..2873)
                     /locus_tag="VFK23_07440"
     CDS             complement(2556..2873)
                     /locus_tag="VFK23_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318948.1"
                     /translation="MSHEQEKPQRCGWIAIGLVLLLLQGILPGSILGGIAGLKSAGMM
                     FGLHTGSDLLNRLFVLGGMITGLMVSAIAIMTLTLAVSGISAGFLKRSSLVASKAKPD
                     TEE"
     gene            complement(2898..>3132)
                     /locus_tag="VFK23_07445"
     CDS             complement(2898..>3132)
                     /locus_tag="VFK23_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318949.1"
                     /translation="GWKERGDEEGSGQGHRGYQEQDIPLDLIMAPSWKGTLAPDIDRP
                     EKLFYTSKQLNFFLEPGMACVISLRVKKTVRLR"
BASE COUNT          675 a          911 c          843 g          703 t
ORIGIN      
        1 cgaacacgcg gtggttccag ggcgaggaga cggaaggaga gctgcgcggg cactttgcgc
       61 tccagcttgc gggccttgcc gatatctacg tgaacgacgc gttcggctcg tggcagcccc
      121 acgcatccac ctcctccatc gtcagcctcc tcccctccta cgcgggcttc ctgctccagc
      181 aggagatcgc gcatcttgac ggcgtgatga ggcccgaacg gcccttcctt gcagtcgtgg
      241 ccggttcgaa gtacgacacc aagatccgtc ccctcaacgc catctacgac aaggtggacc
      301 gcctcgtcct gggcggcgtt atttacaaca cctatctctg cgccaagtat ggcgtccgga
      361 tcaggggagt gagcgacgag gaccgcgatc tcgcacggga cctggtcgaa aaggaccggc
      421 aggcgcaaaa gatcgtcgag cttccgctcg tcgtggaatc ggacgttcct gacgccagga
      481 tcgacggcaa gtaccgcacc atctccgtca gggacttcaa gccgggcggc tccttcggat
      541 atgtcctgga catcgacccc cggtcctttg aggcgacggc cgttgccgag gccatcggca
      601 cggcaaagac catcttcgtg aacgcggtca tgggatatac tccccacttc accgccggct
      661 cccaggcgct cgacaacacc atcgatcaaa accgcgacgc catgaaaatg tacggcgggg
      721 gggacacgct ccaggagttc aaaaacctcc tgcccggcct ctacctttcg gtcatggatg
      781 acgcgagcta ctacttcttc accggtggcg gcacggtgct cacggcaatc gagcacggaa
      841 gcccttacag tctcgaaccg gtcaaggcat tgatggaggc gaaaagaaaa taacggcgct
      901 ttaatagaac agcacatagc aaagttgaaa aatgtcactt ctctttccca tttcctgcag
      961 ctaacccggc tttcaatccc aagaaggatc cgcatttaat gattaataag tcgcgaaact
     1021 tttagactaa atagcgccta atctccttat ttgttactat ttgtaacaca aaaagggttt
     1081 tttatgttat attgagcgta aaaactggat caacagttag ttgtcaggtc atttgcttca
     1141 agtcggcaat aacaaatgaa tgaaagagag gagcgcaaat gggaaagaaa ggctggctcg
     1201 tgacaattgc cggggttgtg atgaacctgg cgctcgggat cctctatgcg tggagcatgt
     1261 tcagtaagca gctcacggaa gcagtagaga agggaggctt cgggtggtcg aagacgcagg
     1321 ctacgctccc ctataccgtt gctattgcct gcttcgcggt catgatggtg cctgcgggcc
     1381 gcttgcagga ccgcttcggt ccccgtatcg tcgccacgat cggcgcggtt ctctgcggcc
     1441 tgggtctcgt tgtagcgagc ctcggcaacc ccgggtccat cctccccgcc atccttggtt
     1501 ttggagtttt ggccggggcc ggcatcggcc tcggatatgc gtcggccaca cccgcagcag
     1561 tcaagtggtt cccgccggaa aagaaggggc tcattaccgg actggttgtc gcgggcttcg
     1621 gcctggcgcc cgtctatatc gcgccgctgt cgaaatacct gcttggaacg tatgggatca
     1681 gctcttcatt cagaatcctc ggctttgcct tcatcatcgc cgcgggaatc gcagcgcaat
     1741 tcatcacgaa cccgacagcg cctgccgcgg ttaacaaatc cggcccgaaa aaccccccgt
     1801 cgcaggactt tacctggcgc gagatgctga ggaccccggc cttttattcc ctgtatattc
     1861 agtatgcatg cgctgcgaca gccggcctta tgataatcgg ccacctggca aagatcgtcg
     1921 ccgtgcaatc gggcaatacg ataaagatcg gattcctctt cgttgccctc ctcgcgattt
     1981 tcaatgcagg cggccgtgtg gtggcgggaa tcgtttccga ttacatcggc aggatcatca
     2041 ctctcgtctt cgtctgcatc atgcaggcca cggtcatggc ttttttcccg cagttctcca
     2101 cgatttcggg tttcatgatc ggttccgcac tggtaggctt caattacggc gcgtgtctct
     2161 ccctcttccc gtccacgacc gcagattact gggggacgaa aaatctcggg atgaattacg
     2221 gcattctctt tactgcatgg ggtgtcggcg gaatcctcgg tcccatgctg gcggggtcga
     2281 ttgccgatgc cagcggatcc tacgctatgg catactatat cgcctgcggg cttttggcat
     2341 ttgcggcgtt tttcgggatg gccagctata taagcttctc ggtaagcctg ccgcaaaagc
     2401 agatcgtggt cagtttctcg ggaaaatcgg caaagaaaaa agaagcgatt gttgcccctg
     2461 cccttgatgg cggtaaaaaa gaagagatgg tggcgtgacg tgggaaaagc ggaagaggat
     2521 cattgagcac gattcggcgc cgacagctct gcctgctatt cttccgtgtc aggctttgct
     2581 ttcgacgcaa caagtgagga ccgcttgagg aaaccagcgg aaatcccgct cactgccagc
     2641 gtcaaggtca tgatggcgat cgccgacacc ataaggcccg ttatcattcc tccgaggacg
     2701 aacaaccggt tgaggagatc cgagcccgta tgaagtccga acatcatccc ggccgacttc
     2761 agacctgcga ttccacccag gatgctgccc ggaagtatcc cctggagcaa cagcaggacg
     2821 agaccgattg cgatccaccc gcaccgctgc ggtttttcct gctcatgcga catgactgca
     2881 ccatccacaa aagtaattca tcttaatctg acggtttttt ttacgcgcaa agatatcacg
     2941 catgccatcc ccggttcaag aaaaaaattg agttgttttg atgtatagaa caatttctca
     3001 ggccgatcga tatctggcgc cagcgtgcct ttccaggacg gcgccatgat cagatcaagg
     3061 ggaatatctt gttcctgata tccccgatga ccctgtccac ttccttcctc atcgcctctt
     3121 tctttccacc ct
//
LOCUS       DASSLD010000409         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_879268,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000409 DASSLD010000000
VERSION     DASSLD010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879268"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VFK23_07450"
     CDS             complement(<1..471)
                     /locus_tag="VFK23_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7318950.1"
                     /translation="MANKILIVDDEPDIVELVTYNLKKDGFRVATASDGEEALHKIRK
                     DKFDLVVLDLMLPGIQGVEICRIIRNDPKTAGTPIIMLTAKGEEVDRVIGLESGADDY
                     MTKPFSPRELIARIKAVLRRTGEKTPAEKIVRIGKLVINKETFGVIKGNTPLDLS"
     gene            737..>2272
                     /locus_tag="VFK23_07455"
     CDS             737..>2272
                     /locus_tag="VFK23_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318951.1"
                     /translation="MKSIARRLYPRLFLFAAFAFLTTCGGSGGGGAPSAGNPPDEYDA
                     VLLSTTKPLSADTLAGLAEVSADGTFRFTNPSPEALNVAPDDVIIGGPSDKTPHGFIR
                     FVISAGTNTQGDLVLSTIPAPIQAAFQKLHVKFTRSIPDIGAAATGRPASPLRFSKFT
                     GGAGSSLTVDYFPFNGDKDPLTEDDQVHVTGTLSGDLEYTFGIDVDWADVVDIPKKIA
                     DCAHQVLTNPFSACDPIPKATVTLSVSGSLNADLAAKGVSFLPYTKDVPVYGPVPLTP
                     FAIGPILWFFPDFEISSKIEGAASSQFATGLSMTADAGVGVSISNKDYVPHFNPDPFA
                     KVTTAAPTVDATLDAHSKVRLGPRVSIKLLDFAGPRAGLFGFAELTASQNNVLQGKPC
                     YTLNGGLEGELGFEIGVDFGIKTVTLAKWDLTQTLFTHEETSGACTGGSNVATNPLQN
                     PTFTPWSNAYSNMVTTTGLPFSAPGGGIAGTALEQTIDGNFVVAGSGSMGLLKIDVQG
                     NAVW"
BASE COUNT          465 a          670 c          624 g          513 t
ORIGIN      
        1 gctgaggtcc aacggggtgt tgcccttgat gacgccgaag gtttccttgt tgatgacgag
       61 cttgccgatg cggacgatct tctccgccgg cgtcttttcg cccgttctcc gcaaaacggc
      121 cttgatgcgc gcaatgagtt cccgcggact gaagggcttc gtcatgtagt cgtcggcccc
      181 gctttcgagg cctatgacac ggtccacctc ttcacccttg gccgtcagca tgatgatcgg
      241 ggtcccggct gttttcggat cgttccttat gatccggcag atttccacgc cctggatgcc
      301 cgggagcatg aggtcgagta cgacaagatc aaatttgtcc tttctgatct tgtggagggc
      361 ctcttcgccg tctgaggcag tcgcgaccct gaagccgtct tttttcaggt tataggtcac
      421 gagttcgacg atatctggct cgtcatcgac gatgagtatc ttattcgcca tgacaatatt
      481 tataacattt atcttcggca aaattcaagc atggcgggaa gatttaacag gactgtaaca
      541 ttcgagatga gtcccgggtt cgtccctgtt tcccctcaag aagaaaatgt agatcgtgta
      601 cagccaaaaa tgcaaattga cacggaaagt agcagatgat aaggtgctgc tgagaaacag
      661 agacttttcc cgttttcggt caagtcagac ccttcgagct attcagacgt gtggatgagt
      721 agaagaggag gatcccatga aaagcattgc ccgtcgtctt tatccgcgtc tgttcctgtt
      781 tgcagccttt gcttttctta ctacctgtgg cggatccggg ggcggcggcg ctccttccgc
      841 aggaaatccc ccggatgaat atgacgcagt cctgctcagc acgacgaagc ctttaagcgc
      901 cgacacgctt gctggcctcg ccgaggtctc ggcagacgga acattccgtt tcaccaatcc
      961 ttctccagaa gcattgaatg tggcgccgga tgacgtgatc atcggaggcc cgagcgacaa
     1021 gaccccccac ggtttcatcc gctttgtcat cagtgccggg acgaacaccc agggagacct
     1081 ggttctttcc accatacccg cgcctatcca ggcggctttt cagaaactgc acgtcaagtt
     1141 cacccgctcc atcccggata tcggcgcggc ggcgaccggc cggcctgcgt cgcctctgcg
     1201 gttttccaag ttcaccggcg gggccgggtc gtcgttaacc gtggactact tccccttcaa
     1261 cggagacaag gaccccctga ccgaggacga tcaggtccat gttacgggca cactgagcgg
     1321 cgacctggag tacaccttcg ggatcgacgt ggactgggcc gacgtggtgg acattccaaa
     1381 aaaaatcgcg gattgcgcgc atcaggttct tacaaaccct ttctccgcct gcgaccccat
     1441 cccgaaggcg accgtcacct tgtcggtttc gggaagcctc aatgcggacc tggcggccaa
     1501 gggcgtgagt tttctgccct atacaaaaga tgtcccggta tacgggccgg tccctttgac
     1561 gcccttcgcg atcggaccga ttctctggtt ctttcccgac ttcgagattt cgtcgaagat
     1621 cgaaggcgcg gcatcgagtc agttcgcgac gggactgtcc atgacggccg atgccggcgt
     1681 gggcgtctcc atatcgaata aggattatgt cccgcacttc aatcccgacc ctttcgcgaa
     1741 ggtcaccacc gccgctccga ccgttgacgc cacgcttgac gcccattcaa aggtgcggct
     1801 cggaccgcgg gtttcgatca agcttctgga tttcgccggt ccgcgcgcgg ggctcttcgg
     1861 cttcgccgaa cttacagcca gccagaacaa tgttctgcaa gggaaaccct gttatacctt
     1921 gaacggggga ttggaaggtg aactgggctt cgagatcggc gtggatttcg gcatcaaaac
     1981 cgtgaccctc gctaaatggg acttgacgca aaccctcttt acccatgaag aaacaagcgg
     2041 tgcctgcacc ggcgggagca atgtggcaac gaaccccctg cagaacccca cctttacccc
     2101 ctggtcaaat gcctacagca atatggtcac gaccaccggc ttgcctttct cagcccccgg
     2161 cggcggtatt gccggaacag ccttggaaca aaccatagac ggcaacttcg tcgtggccgg
     2221 gtccgggagc atgggactcc tgaagatcga tgtgcagggc aatgcagtat gg
//
LOCUS       DASSLD010000410         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_253042,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000410 DASSLD010000000
VERSION     DASSLD010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253042"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..>2086)
                     /locus_tag="VFK23_07460"
     CDS             complement(171..>2086)
                     /locus_tag="VFK23_07460"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HET7318952.1"
                     /translation="GFDGTYSADLEISGVGLSSVDHMAGTDLDGDANLRLHTAGSFST
                     PVLNATFAVEKFSFRGTGMGTLRGSVDIKDHALKAEAGLPEDAASLTLDWMLRKPYTW
                     TAEAEVKTDGFNPLLLFGNAALAERVKLIAEGGIRARGTGLDRSSVSGDASFSRIAVV
                     IGEYRIDSESQPALTISRGRLAARSLDFAGPSTRFSVSGATDLSTEIDVAVKGTANLP
                     ILRLFFPEVEHATGTAEMKLTVTDEWSNPDVAGELRIRDGEIKLADIPQRFTALNGTI
                     VFTQGRIMTESLTGEIGGGSLAASGWVQLSGVTIEDFSVKTMVDSVQVRYPEGLTSTL
                     SGDLYFDGGTQGRSLSGDIVIKRARYDKPIEWKSMLVSFGRGLYQKKKTEIGWIGETQ
                     INVRFHGSENILFQNNLAKMQLDIDVFLRGTVNHPQFLGRIETRKGSVFLRKNEFKIV
                     RASADFIDPNRINPVLDIQAETQVREYQIQLSITGTAERASVTLVSDPALNDTDIQSL
                     LALGKKGSELRGKGESNVGIGEAASFATGLFQDVLERRAKSLTGLDRFQVDPYVSKND
                     TSVPRVTAGKELIQNKLFVTYSSNVGAPAPEQNFRIEYILNKHFSLVGEGKETINNGT
                     DIGADIKYRFEFQ"
BASE COUNT          404 a          622 c          608 g          452 t
ORIGIN      
        1 aggagtgatc gcgctttcag tctctatcaa ggtggacatg ggcgcgggaa agaactggga
       61 cgaggcgcac tgattcaagt aagaaggatg aattaagaat taagaattaa gaattaagaa
      121 ttaagaatta agaattaaga attaagaaat agcctttgat cttacttgcc ttattgaaac
      181 tcaaaccggt acttgatgtc cgccccgata tccgttccgt tgttgatcgt ctctttcccc
      241 tcgcccacga gcgaaaagtg cttgttcagg atatattcga tcctgaaatt ctgttccggc
      301 gcaggggcgc ccacattcga ggaataggtg acgaaaagct tgttttgaat gagttccttc
      361 cccgccgtga cccgtggcac cgatgtgtcg ttcttgctga catacggatc aacctggaaa
      421 cggtcgagtc cggtcaggct cttcgcgcgg cgctccagga cgtcctggaa cagccccgtt
      481 gcgaatgacg cggcctctcc gatgccaacg tttgactcgc cttttccccg gagctccgag
      541 cctttcttgc cgagggcgag aaggctctga atgtcggtgt cgttcagggc ggggtcggag
      601 acgagcgtga cggaggcgcg ctcggccgtg cctgtgatgg acagctggat ctggtactcg
      661 cgcacctgcg tctccgcctg gatgtcgagc acggggttta tccggttcgg gtcgatgaaa
      721 tcggcggacg cacggacgat cttgaattcg ttcttccgga gaaagacgga ccctttgcgc
      781 gtctcgatcc tgcccaaaaa ctgcggatgg tttaccgtgc cgcgaaggaa cacatcgata
      841 tcgagctgca tcttggccag attgttctgg aacaggatgt tttcgctgcc gtggaagcgg
      901 acgttgatct gcgtctcccc gatccatccg atctcggtct ttttcttctg gtacaatccc
      961 ctgccgaagc tcaccagcat gcttttccat tctatgggct tgtcgtatct cgcccgtttg
     1021 atcacgatgt ccccggacag ggaccgcccc tgcgtccctc cgtcgaagta caggtctccc
     1081 gaaagcgtgg aggtcagccc ctccgggtag cggacctgca cactgtcgac cattgtcttg
     1141 accgagaaat cctcgatcgt gaccccggaa agctgcaccc agcccgaagc ggccagcgag
     1201 cccccgccta tttcgccggt caaggattcc gtcatgatcc tgccctgggt gaaaacaatg
     1261 gtcccgttca gcgccgtgaa cctttggggt atatccgcca acttgatttc gccgtcccga
     1321 atgcgcagtt ccccggccac gtcgggattg ctccattcgt ccgtgaccgt gagcttcatc
     1381 tcggccgtgc ccgtggcgtg ctcgacttcg gggaagaaca gcctcaaaat gggaaggttc
     1441 gcggtaccct ttaccgccac gtcgatttcg gtcgaaaggt cagtcgctcc cgaaacgcta
     1501 aaccgagtgg aggggccggc gaaatccagc gacctggccg ccagcctgcc cctgcttatg
     1561 gtgagcgcgg gctgcgattc gctgtcgatg cggtattcgc cgatgaccac ggcgatgcgg
     1621 gaaaaggacg catcgcccga tacggaagag cggtcgagcc cggtgccgcg ggccctgatg
     1681 ccgccctcgg cgatcagttt cacccgctcg gcgagcgcgg cgttgccgaa caagaggagc
     1741 ggattgaaac cgtcggtctt cacctcagcc tccgcggtcc aggtgtaggg cttccggagc
     1801 atccagtcga gggtcaggct tgcggcgtcc tccggcagcc ccgcctcggc cttcagcgcg
     1861 tggtctttga tatcgacact gccgcggagc gttcccatcc cggtcccgcg aaaggaaaac
     1921 ttctcgacag cgaacgtcgc gttcagcacg ggcgtagaga agcttccggc ggtatgcagc
     1981 cgcaggttcg catccccgtc caggtcagtc ccggccatat ggtccacgga tgatagaccg
     2041 acgccgctga tttcgaggtc cgccgaatag gtcccgtcga acccga
//
LOCUS       DASSLD010000411         3071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_850382,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000411 DASSLD010000000
VERSION     DASSLD010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850382"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFK23_07465"
     CDS             <1..194
                     /locus_tag="VFK23_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET7318953.1"
                     /translation="GERNPTPPQYYTSMEMIISVKGTGLTDKKLERAISLSHEKYCSV
                     YHTLRKDMKMKVDYTYENV"
     gene            307..1464
                     /locus_tag="VFK23_07470"
     CDS             307..1464
                     /locus_tag="VFK23_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET7318954.1"
                     /translation="MTTVRLLKNGAEAFPAMFDAMDRAASSISFEMYIIADDGTGREF
                     REHLVGAARRGVEVRGLVDAWGSWNLPDSFWDGLREAGGQVRWFHPLTHGLLPFRNHR
                     KLLLVDVTLAIIGGLNVSDEYYRGANNESPWRDNALEIQGPEVAGLRRSFQRMWAAAD
                     LPLRRLFSLVRRERKTRRRTSGAVRFLESGPENPMRPVRRAYRQVVQNARRTIDLAMG
                     YFYPHGRMIRSLRRAVRRGVRVRLMVPLHTDIPISRWAARGLYGRLLRAGVEVWEYTP
                     SMMHSKLAIADDTVITGSANMDIRSGRINYELVAIVDDPELAARARADFEEDLARSVR
                     IGIEDWRNRSVIQKLKERFSYCLLARFDIFIARREMARGARGRLRAPDKRS"
     gene            1554..1853
                     /locus_tag="VFK23_07475"
     CDS             1554..1853
                     /locus_tag="VFK23_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318955.1"
                     /translation="MDTTNWKVTEAPTCPHCKTKMEQMDSRHLDWESPYLWVCFNNEC
                     TLFKKGWDHMMASVGQLVSYRFMIHPQDGTCGVIPALSHEYMQKFGKPGDPYPEE"
     gene            complement(2005..>3071)
                     /gene="pyk"
                     /locus_tag="VFK23_07480"
     CDS             complement(2005..>3071)
                     /gene="pyk"
                     /locus_tag="VFK23_07480"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET7318956.1"
                     /translation="ILLDDGRLELNVVKKSKAGLLCRIVRGGTLKSHKGVNLPGAKLS
                     LPSLSRKDKEDLRFGIARGVDYIALSFVRTAKDIVSTRRFLKAHGADIPIIAKIEKSE
                     AVQNLDEIIEASDGVMVARGDLGVEMSPEQVPLLQKKIIAACNRAEKPVITATQMLES
                     MIENPQPTRAEASDVANAILDGTDCVMLSGETAMGNYPVQAVEVMARVAREAETILAP
                     APPDAHTSGEGGSVAHAACRAAEELQAQAIVTFTQSGTTALLVAKHRPRMTIIAPTPF
                     DHVARKMSLYWGVTPILLKTKRTTDDMIASVERAMLYRKLARRHDLIVITAGVPIGVA
                     GSTNMMKIHRVGEVRGLETK"
BASE COUNT          575 a          900 c          949 g          647 t
ORIGIN      
        1 tgggcgagcg caatccgacg ccgccccagt attatacctc gatggagatg atcatttcgg
       61 tcaagggcac gggtctcacg gacaagaaac tcgaacgcgc catttccctc tcgcacgaaa
      121 agtactgttc cgtctatcac acgctcagaa aagacatgaa gatgaaggtg gattatacgt
      181 acgagaacgt gtaaacttca gagattctcg gtacttgata ctggatgaaa tcatgatagc
      241 ggcgaatgat cgtctcacag gatgttatcc ggcatcaggc atcaaacatc cggtatcgtt
      301 acgtttatga ccaccgtccg tctcctcaaa aacggcgcag aggcctttcc ggccatgttt
      361 gacgccatgg accgtgcggc atcgtccatc tcgttcgaga tgtacatcat cgccgatgac
      421 gggacggggc gggagttccg cgagcacctc gtcggggcgg cgcgcagggg cgtcgaggtc
      481 aggggccttg ttgacgcgtg gggctcctgg aacctgccgg actcgttctg ggacggactc
      541 cgcgaggccg ggggacaggt gcgctggttt catccactta cccatgggct cctgccgttc
      601 cgaaatcatc gaaaattgct gctggtcgac gtcacccttg ccatcatcgg cggtctgaat
      661 gtctccgatg agtattaccg gggcgcgaac aacgaaagtc cctggcggga caatgccctg
      721 gagatacagg gccccgaggt tgcgggcctg cgccgctcct ttcaacgcat gtgggctgcg
      781 gccgacctgc ctcttcgaag gctgttctca ctggtgcgcc gcgaacgaaa gacccgaaga
      841 agaacgtccg gtgccgtccg gtttctggag agcggtccgg agaatcccat gcgccccgtg
      901 cgcagggcct accggcaggt ggtccagaat gcccggcgca cgatcgacct cgccatgggc
      961 tacttctatc cccacggcag gatgatccgg tccctccggc gcgcggtcag gcggggggtc
     1021 cgcgtacggc tcatggtccc ccttcacacg gacatcccga tcagccgctg ggccgcgcga
     1081 ggcctttacg gcaggctcct ccgcgccggc gtcgaggtct gggaatacac gccttccatg
     1141 atgcattcga agctcgcgat cgccgacgat acggtgatca cggggtcggc gaacatggat
     1201 atccgtagcg gaaggatcaa ctacgagctc gtggcgatcg tggacgatcc cgaactcgcg
     1261 gcccgtgccc gtgccgattt cgaggaggac cttgcaaggt cagtccgcat cgggatcgaa
     1321 gactggcgca accggtccgt catccagaaa ttgaaggagc ggttcagcta ctgcctgctc
     1381 gcgcgtttcg atatattcat cgcaagaaga gagatggcaa ggggggcgcg gggccggctc
     1441 cgggctccgg acaagcggtc ctgagagcgg ggcgcggctt ttcctgttgc aaagggacat
     1501 cggtcgacgt ataatgtctc tcgctcgatt gacggttcac ggaggttttt ttgatggaca
     1561 cgaccaattg gaaggtcacc gaggcgccga cctgtcctca ctgcaagacg aagatggagc
     1621 agatggactc gcggcacctg gattgggagt caccctatct ctgggtttgt ttcaataatg
     1681 aatgcacgct gttcaaaaag gggtgggacc acatgatggc gtccgtcggg caactggtgt
     1741 cttaccgctt catgatccat cctcaggacg gaacgtgcgg agtgatcccg gccttgagcc
     1801 acgagtacat gcagaagttc ggcaaaccgg gagatcccta tccggaagag tgagtttttg
     1861 aaggccgtac atcatttcga aaatctgtcc ttttctacgt cgcatcctct ttgtcattcc
     1921 cgcgaaagcg ggaatccatt ttgaagttta ctcgataaag ccgatggatg cctgctttcg
     1981 caggcatgac aaatgaaagt gccctcactt cgtttccagc cccctcacct ctcccacgcg
     2041 gtggatcttc atcatattcg tgctgcctgc cacgccgatc ggcactcctg cagtgatcac
     2101 gattaggtcg tgccgccttg ccagcttacg atagagcatg gcgcgttcga cgctcgcgat
     2161 catatcgtcg gtggtgcgct tggtcttgag aagaatgggg gtcacgcccc agtagagtga
     2221 catctttctc gccacatggt cgaacggcgt gggggcgatg atcgtcatcc gggggcgatg
     2281 ctttgcgacc aaaagcgcgg tcgtacccga ctgggtgaag gtaacgatcg cctgcgcctg
     2341 cagctcttcg gccgcgcggc aggccgcgtg ggcaacgctc ccgccctcgc cggaggtgtg
     2401 ggcgtcgggc ggcgcgggtg cgagaattgt ttccgcctcc ctggcgaccc ttgccatgac
     2461 ttcaacggcc tggaccggat agttgcccat cgctgtctct cccgaaagca tgacgcagtc
     2521 cgttccgtcg aggatggcgt tcgcgacatc cgaggcctcc gcgcgcgtgg gctgggggtt
     2581 ttcgatcatg ctttcgagca tctgcgtggc cgtgatcacc ggcttctcgg cgcggttaca
     2641 cgccgcgatg atcttcttct ggagcagcgg aacctgctcg ggggacatct caacgcccag
     2701 gtcgccgcgc gcgaccatga cgccgtcgga cgcttcgatg atctcgtcga ggttctgaac
     2761 ggcctcggat ttttcgatct tcgcgatgat cgggatgtcc gcgccgtgcg ctttcaggaa
     2821 ccggcgcgtc gagacgatgt ctttggccgt gcgcacgaac gagagggcga tgtagtccac
     2881 gccccgggcg atgccgaaac ggaggtcttc cttgtccttg cgcgagagcg aagggagcga
     2941 gagcttcgcg ccgggcaggt tgacgccctt gtgacttttg agcgtgcctc cccggacaat
     3001 cctgcagaga aggccggctt tgcttttttt tacgacgttg agttccagcc tgccgtcgtc
     3061 cagcagtatc g
//
LOCUS       DASSLD010000412         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1120789, whole genome shotgun sequence.
ACCESSION   DASSLD010000412 DASSLD010000000
VERSION     DASSLD010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120789"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(338..1621)
                     /gene="eno"
                     /locus_tag="VFK23_07485"
     CDS             complement(338..1621)
                     /gene="eno"
                     /locus_tag="VFK23_07485"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET7318957.1"
                     /translation="MSDIIDVFAREVLDSRGNPTVEADVVLESGVLGRAIVPSGASTG
                     EREALELRDGGARYLGKGVQKAVRNVIEMIGPAVSGFDATEQSAIDQTMIALDGTESK
                     KKLGANAMLAVSLAVAKAAAEDAGLPLYQYLGGPNAKELPVPMMNVLNGGAHADNNVD
                     FQEFMIIPAGAKSCTEAIRTGAEIFHSLKSVLKKKGYNTAVGDEGGFAPNLRSNEEAC
                     QVILEAITKAKYKPGKDVFLGLDVASSEFYDKGVYDMKGEGKKMTSDKMIKFYEEWIR
                     QYPIISIEDGMSEKDWKGWKLLTDRLGGKVQLVGDDIFVTNTSIFKKGIAEGIGNSIL
                     IKLNQIGTLTETLDAIEMAKRAQYTAVVSHRSGESEDATIADLAVATNAGQIKTGSLS
                     RTDRIAKYNQLIRIEEELGDAAVFRGKEVFYSIRK"
     gene            complement(1746..>2747)
                     /locus_tag="VFK23_07490"
     CDS             complement(1746..>2747)
                     /locus_tag="VFK23_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HET7318958.1"
                     /translation="TAPLPLVDAKEKGIVEEVDFRKSYLDDLRKKIDFDTIRKAKMKV
                     ACDLLYGTGRDYLDTVLRDAGCVVTSIHGHRDTMFGGHAPEPSEENLAELSTLMQKGK
                     FDIGLAVDGDADRFGVLDTDGAYINPNQVLALVLDYLCRTRGWKGGAARSIATSHLID
                     AVAKMHGIEVYETGVGFKYIGDLLVQGKIIFGGEESAGMTIKDHVPEKDGIIACLLVA
                     EMVAHEKKSIADMLKRLYKEVGTILNERVNIRLTEANRAQVQARLSQPLTELGGLRVK
                     GKKTTADGTKYMLEDDSWVLMRASGTEPVVRVYVESSSGEKIRDLIEAGKKFILGES"
BASE COUNT          515 a          802 c          747 g          683 t
ORIGIN      
        1 taagttgttc atgtgattgc cattgtaaga cacattaacc actctagtca gatattagaa
       61 tcaattttcg gtcctctgtc aatacccaat gatgggtata aatggggtag tcagacaatc
      121 caaagatatg aaaaataatg gaacgtgatc aatttgttgc agaaataggg tgtgaggggt
      181 gttgagtgta attgcggtat gctgtacggg gtatactgag ttgacgagca caggatgtac
      241 cgctcttgta atttctttat cgtggcacct ctgtttcaaa acaaaaagcc ggagaaccag
      301 aggcgtcctc cggtctccgg cttcgggaag taaggtgtta cttcctgatg ctgtaaaaaa
      361 cttccttgcc gcggaacact gccgcgtctc ccagttcttc ctcgatgcgg ataagctggt
      421 tgtacttggc gatccggtcg gtccgtgaga gagagccggt cttgatctgg cctgcgttcg
      481 tggcgaccgc gaggtcggcg atcgtcgcgt cctcgctctc accggagcgg tgcgacacga
      541 cagcggtata ctgtgcccgt ttcgccattt cgatggcatc cagggtctcg gtaagggtcc
      601 cgatctggtt cagctttatg aggatcgagt tgccgatgcc ttcggcaatg ccttttttaa
      661 agatcgaggt attggtcaca aagatgtcat cgcccacgag ctggaccttg ccgcccagtc
      721 ggtcggtcaa cagcttccag cccttccagt ccttttcgga catgccgtcc tcgatgctga
      781 tgatgggata ctgccggatc cactcctcgt agaatttgat catcttgtcg gacgtcattt
      841 tcttgccttc gcccttcata tcgtagacgc ccttatcgta gaactcgctt gacgccacat
      901 cgagtcctaa aaacacgtcc ttgccgggtt tatacttggc tttggtgatc gcctcgagga
      961 taacctggca ggcttcttcg tttgaacgga gattcggcgc aaaaccgccc tcgtctccta
     1021 ccgccgtatt gtagcccttc tttttgagaa cggacttgag gctgtggaaa atctccgcgc
     1081 ccgtccggat agcctcggtg caggacttgg cgccggcggg gatgatcatg aattcctgga
     1141 agtccacgtt gttatccgca tgcgccccgc cgttcagcac gttcatcatc ggcacgggaa
     1201 gttccttggc gttcggtcct cccaggtact ggtagagcgg cagccccgcg tcctcggctg
     1261 cggctttggc caccgcgagg gacaccgcaa gcatggcgtt ggcccccagt ttctttttgc
     1321 tttcggtccc gtcgagggcg atcatggtct ggtcgattgc ggattgctcg gtcgcgtcaa
     1381 aacctgatac cgcgggcccg atcatttcaa tgacgttccg gaccgccttt tgaacgccct
     1441 tgccgagata ccgtgcgccg ccatcacgga gctcgagcgc ttcgcgttct cccgtcgacg
     1501 ccccggaggg tacgatggcg cgccccagca caccgctctc gagtacgacg tcggcctcca
     1561 ccgtggggtt gccccgtgaa tccagtacct cgcgtgcaaa tacatcgatg atgtcgctca
     1621 tgaaaccccc ttatgaacat aattcggaat gtggaatgcg gaattcggaa taaaagcaaa
     1681 aatcaggcaa tcgaccgaat cttttcaatc ccgcattccg aattccgaat tccgaattcc
     1741 gcattttatg attcccccag aatgaacttc ttccccgcct cgatcaggtc cctgatcttt
     1801 tctccggatg aggactctac gtacaccctg accactggct ctgttcccga cgcgcgcatc
     1861 agcacccagc tgtcgtcctc gagcatgtac ttggttccgt cggccgtggt cttttttcct
     1921 ttgacccgaa gcccgcccag ttctgtgagc ggctggctga gccgcgcctg gacctgcgcg
     1981 cgattcgcct ccgtgaggcg gatgttgacc cgttcgttca ggatcgttcc tacttccttg
     2041 tacagccgct tcagcatgtc cgcaatggat tttttctcgt gggcgaccat ctccgcgaca
     2101 agcagacagg cgataatgcc gtctttttcg ggaacgtggt ccttgatcgt catgccggcg
     2161 ctctcctccc cgccgaagat gatcttgccc tgcacgagga ggtctccgat gtacttgaag
     2221 ccgacgccgg tctcatacac ctcgattccg tgcatcttgg cgactgcgtc aatcaggtgt
     2281 gacgtcgcga tggagcgggc ggccccgccc ttccacccgc gggtcctgca gagatagtcg
     2341 agcaccagcg caaggacctg gttggggttg atataggcgc cgtccgtgtc aaggaccccg
     2401 aagcggtccg cgtcgccgtc tacggcaagg ccgatgtcga acttcccttt ctgcatcagc
     2461 gtcgacaact ctgccaggtt ttcctcggac ggttcggggg catggccgcc gaacatcgtg
     2521 tcgcggtgac cgtgaatgga ggtcacgacg catcccgcgt cccggagcac cgtgtcgaga
     2581 tagtccctgc ccgttccgta gaggagatcg cacgcgacct tcatttttgc tttccggatc
     2641 gtatcgaagt cgatcttttt tctgagatcg tcgaggtagg acttgcggaa atcgacttcc
     2701 tccacgatgc ccttctcctt tgcgtcgacg aggggaagag gcgccgt
//
LOCUS       DASSLD010000413         5599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_663485,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000413 DASSLD010000000
VERSION     DASSLD010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663485"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..679)
                     /gene="bfr"
                     /locus_tag="VFK23_07495"
     CDS             complement(212..679)
                     /gene="bfr"
                     /locus_tag="VFK23_07495"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HET7318959.1"
                     /translation="MKGNPKLIETLNSLLADELTAINQYMVHSEIAANWGYGKLHEHF
                     EKRAIAEMKHAEKLIGRITFLEGAPTVSNLRKIMIGSEVPKQLEYDHAAEEGAIKAYN
                     AGIQLAGEVGDFATREILEHILNDEDAHIDGIEELQDQISHMTLPVFLTTQVG"
     gene            complement(841..981)
                     /locus_tag="VFK23_07500"
     CDS             complement(841..981)
                     /locus_tag="VFK23_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HET7318960.1"
                     /translation="MGLSDMVWMGLIIAGALYLLYRSVWKKKGHCTGCDSRTCELRKS
                     KI"
     gene            complement(983..3169)
                     /gene="feoB"
                     /locus_tag="VFK23_07505"
     CDS             complement(983..3169)
                     /gene="feoB"
                     /locus_tag="VFK23_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HET7318961.1"
                     /translation="MKRAASQRKTVTVAVAGNPNSGKSTLINAIAGTRLHVGNWPGVT
                     VEKKEAVFEYEGTRIRLVDLPGVYSLSPYTQEEIITRDYLVHELPDVIINVVDATNLE
                     RNLYLTVQLLELEIPVVMALNIYDEAEKKGYVIDVKLMEETLGVKVVPTVATRKNGLQ
                     DLLKAVHKLTDAASPYPRELNYGEDIESAAGAVVKRIKDGYPALAEKYPLRWLAFKIM
                     EEDEQAQKELNISGDELMIKDAVRHLKKAHEEDIESVMADARYAQAAGLTREVLKRPE
                     LRKTELTEKIDRIALNRFLGIPLFLAAMWFMFKLTFDVSKPFGDWMNAMTSGPFKRWA
                     EMLVGLVHAPAWISSLVTDGVIAGVGSVLVFVPVIFAMMFFITFLEGSGYMARAAFVM
                     DRAMHAIGLHGKSFIPMLLGFGCNVPAIYATRTLENQRDKALTALLVPLMSCGARLPV
                     YVVFIGAFFPAHAGTVLWSLYVMGIALAIVMGLIFKKTLFRGEAPMFIMELPPYRMPS
                     LKSLMIHTWEKGKHFLVKAGTYILAVSILVWFLLNLPWGVENKKDSYLGQVGQVAAPL
                     LKPLGFGNWEAASSLMAGVIAKEIVVGTMGEIYVQKGEEEMNAVKPAFTDDLKELGTS
                     FAAALTNSAKNVFSTFRISSLSAEEDEKRAGLKSVLRGVFTPLSAYAFITFVLLYMPC
                     VVVAIAMRQEFGTWKWFGIAFLYQMSLAWLAAFVIYQGGRLLGIGG"
     gene            complement(3223..3447)
                     /locus_tag="VFK23_07510"
     CDS             complement(3223..3447)
                     /locus_tag="VFK23_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HET7318962.1"
                     /translation="MNLSLLKPGESGKITKIGAIGPLKRRLMDMGVLVGESVKVEKIA
                     PLGDPIEVTIKQYKLSLRKKEAEEIAVEAL"
     gene            complement(3444..3674)
                     /locus_tag="VFK23_07515"
     CDS             complement(3444..3674)
                     /locus_tag="VFK23_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HET7318963.1"
                     /translation="MVPLGLLGIGERAEVMDVLSEKHPGARMEDMGFRAGKTVEMLNN
                     AGGGPLLVKIDESRLALSRGTAMKIMVRRKKR"
     gene            complement(3685..4467)
                     /locus_tag="VFK23_07520"
     CDS             complement(3685..4467)
                     /locus_tag="VFK23_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET7318964.1"
                     /translation="MLKTSLVAIVTIACALAGEAFAAHPLITDDSGTQGKGNVQLEFI
                     GEYGIDKHDGVREKSLQVPTVPFLTYGLTDATDIVMGLPYNEVVTEDAAGKSAVRGIG
                     DASLELKTRFYEKDGLSFAVKPGITLPTGDENKGLGNGRVSYHAFLITTKEVQPCAFH
                     LNLGYIHNQYKFQADEDANRKDLWHVSLAAQAELVKNVKAVANIGMERNPDKTSEAHP
                     AFILGGLICSITENLDIDAGIKYGLNRPETDIAYLAGITWRL"
     gene            complement(4526..5233)
                     /locus_tag="VFK23_07525"
     CDS             complement(4526..5233)
                     /locus_tag="VFK23_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318965.1"
                     /translation="MKKLFCSAAVLFLITFMGGAVQAAQRNFVWTEEYGTLAKGNAEV
                     EFWSTAVTEDIQTRNASDWTQQVELEYGVTDRFNVALYQVYEQAADEPSLTYLGYKVE
                     LKYRVAEKNALPVDVLLYAEDEHLNDEDGPRFEGKLILAKEIGRVGMAYNQVFERRYA
                     SGKEEHEYAAGINYEVLPSFRFGVESKGSYSENQYAVGPTLAWVGNRIWADIGAVYGL
                     NKKTDDRQVRFLLGVPF"
     gene            complement(5389..>5599)
                     /locus_tag="VFK23_07530"
     CDS             complement(5389..>5599)
                     /locus_tag="VFK23_07530"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HET7318966.1"
                     /translation="ADLMPGEQAKIVFITPESHSRLDRLTAMGLSPGSMVKLHQKKPS
                     YVIQLGETMIAVDKDITKEIFVKKM"
BASE COUNT         1172 a         1680 c         1383 g         1364 t
ORIGIN      
        1 aaactcctga tgggaagctc cacggaaaag gtcatcggat cggcaggctg cgccgtgctc
       61 gtagccaagg cgtagtgcgc atcgtaaaaa aaaggcaatg gcagaagcca ttgccttttc
      121 ccgatcgaat gattcaaaaa tattcaaccc ctcgatctca atactccgca aaaaataccg
      181 cagaccctct tctccgtcca acgggctttt cctacccgac ttgcgtcgta agaaataccg
      241 gcagcgtcat gtgggagatc tggtcctgga gttcctcgat gccgtcgata tgagcgtcct
      301 cgtcgttcag aatgtgctcg agtatctcgc gcgtggcaaa atcgcctact tcccccgcca
      361 gttgtatgcc tgcgttgtac gccttgatgg caccttcttc ggcggcatgg tcatactcga
      421 gttgcttggg cacttcgctg ccgatcatga tcttcctcag attggagact gtgggagcgc
      481 cttcgagaaa agttatccgt ccgataagtt tttccgcgtg cttcatctcg gcaattgccc
      541 ttttttcaaa gtgctcgtgc agcttcccgt agccccagtt ggcggctatc tcggaatgca
      601 ccatgtactg gttgatcgcc gtcagttcgt cggcaagcag cgagttcaac gtttcgatca
      661 gtttcgggtt ccctttcatt tcgtatcctc ctccgtttct cgttgtcaaa ccggcctttc
      721 cggtccttgg ccgcgtccgc gattacttgt aagagtatag caaggttccc ggaaaaatca
      781 aggttacaac catcttcggg cgggaaaaat gcagaggagg gatcccgctt cgcgtcgggc
      841 ttatatcttt gattttctga gttcgcaggt ccgtgaatcg caccctgtgc agtgcccctt
      901 cttcttccaa acggagcggt acaaaagata aagcgcgccc gcaatgatca gccccatcca
      961 caccatgtcc gacaatccca tgtcaacctc cgattcccag gagtcttcct ccctggtaga
     1021 tcacaaacgc cgcaagccat gcaaggctca tctgatacag aaaggcgatg ccgaaccatt
     1081 tccatgttcc gaactcctgc ctcatcgcaa tggcgaccac gacgcagggc atatagagca
     1141 ggacgaaggt gatgaaggcg tatgccgaaa ggggcgtgaa gactccccgg agtacggact
     1201 taaggccggc ccgtttttcg tcctcttccg cggaaagact cgaaattcta aaggtcgaga
     1261 aaacgttctt tgcggaattc gtaagagcgg cggcgaagga cgtcccaagt tccttgagat
     1321 catcggtgaa tgccggcttc acggcattca tttcctcttc gcctttctgc acatatattt
     1381 cacccatcgt tcccacgaca atttccttgg cgattacacc cgccatgagc gatgaagctg
     1441 cctcccagtt tccgaacccg aggggtttga gcagcggggc ggccacctga cccacctgtc
     1501 ccagatagga gtctttcttg ttctcgacgc cccagggaag attcagcagg aaccacacca
     1561 gtatcgatac agccaggata tacgtccccg ccttgaccag gaagtgcttt cccttttccc
     1621 acgtgtggat catgaggctt ttcaaagacg gcatcctgta gggaggcagt tccatgatga
     1681 acatgggcgc ctctcctctg aaaagggtct ttttgaaaat cagccccatg acgatcgcca
     1741 aggcgatacc catgacatac aaggaccaca aaaccgtgcc cgcatgggct gggaaaaaag
     1801 cgccgataaa taccacatat accggcagcc gggcgccgca ggacataagg ggaacgagca
     1861 aggccgtaag ggccttgtcc cgctggtttt cgagcgtgcg ggttgcgtag atggccggga
     1921 cattgcagcc gaagccgaga agcatgggaa tgaacgactt gccgtggagc ccaatggcat
     1981 gcatggccct gtccatgaca aaggccgcgc gcgccatgta tccgctcccc tcaaggaagg
     2041 tgataaagaa catcatggcg aagatgaccg gcacgaacac gagcacggag cccacgcccg
     2101 cgatgacgcc gtcggttacg agggaggata tccaggccgg cgcatggaca aggccgacga
     2161 gcatttccgc ccatctcttg aaaggccccg aggtcatggc gttcatccag tcgccaaagg
     2221 gtttcgagac gtcaaaggtc agcttgaaca tgaaccacat ggcagcaaga aagagaggaa
     2281 tgcctaggaa ccggttcagg gcgatccggt cgatcttttc cgtcagttcc gtcttccgga
     2341 gctcgggtcg tttcaggacc tcgcgggtaa gcccggctgc ctgcgcatac cgcgcgtcgg
     2401 ccatgaccga ttctatatct tcctcatgcg cctttttgag atggcggacg gcatccttga
     2461 tcatcagctc atcgccgctg atattcaatt ccttctgcgc ctgttcgtct tcttccatga
     2521 tcttgaaggc gagccacctg agcggatact tctccgcgag cgccgggtat ccgtctttta
     2581 tccgtttcac aacggcccct gcggcggatt cgatgtcttc gccgtagttc aactccctgg
     2641 gataaggaga cgcggcatct gtaagtttgt gaacggcctt caggaggtcc tgcaaaccgt
     2701 tcttcctggt tgcgacggtc gggaccactt tcaccccaag cgtctcttcc atcaatttta
     2761 cgtctatgac ataccccttc ttttccgcct cgtcgtaaat attgagcgcc atgacgacgg
     2821 gtatctccag ttcgagaagc tggaccgtca ggtaaaggtt tctctccagg ttggttgcat
     2881 ccacgacatt aataatgaca tccggcagct catggacgag ataatcccgc gtgatgattt
     2941 cctcctgggt atagggactg aggctgtaga cacccggcag atcaacgagc ctgatccgtg
     3001 tcccttcata ttcgaagacg gcttcctttt tctctaccgt aactcccggc cagttgccca
     3061 cgtgcagcct cgtccccgcg atggcgttga tgagggtgga tttgcccgaa ttgggattcc
     3121 ccgccaccgc aacggtgacc gttttccgct gactcgcggc ccgcttcatc ggggcactct
     3181 gctccggttt taattccgca ttccgaattt cgcattccgc attcataacg cctccaccgc
     3241 tatctcttcc gcctctttct ttctcaagga aagtttgtac tgtttaatcg tcacctcgat
     3301 ggggtcgccc agcggcgcaa ttttttccac tttcacactc tcacccacga gcacacccat
     3361 gtccatcagc cttctcttca gcgggccgat tgcgcctatc ttcgtgatct tcccgctctc
     3421 gccgggcttc aacagagaca gattcatcgt tttttcctcc ttaccattat tttcatggcc
     3481 gtcccccgac tcagggcgag ccttgattca tcgattttta ccaggagcgg gccgcctccc
     3541 gcgttattca acatctccac ggtttttccc gcgcgaaagc ccatgtcttc catccgcgcg
     3601 ccgggatgtt tttccgaaag gacatccatc acttccgctc tttcgccgat ccctaataaa
     3661 cccaggggca ccatgaccca cctcctataa tctccaggtg atccctgcga gatatgcaat
     3721 gtccgtctcc ggacggttca gcccgtattt tattcctgca tcgatatcga gattctcagt
     3781 aatcgaacag ataagaccgc ccaggatgaa cgcgggatgg gcctccgatg tcttgtcggg
     3841 gtttctttcc atcccgatat tcgccacggc cttcacgttc ttgacgagct ccgcctgcgc
     3901 tgcaagggac acgtgccaga gatcctttct gttggcgtcc tcgtccgcct ggaatttata
     3961 ttgattgtgt atataaccga gattcagatg aaaagcacag ggctgcacct ctttcgtcgt
     4021 aatgagaaag gcatggtagg ataccctgcc gtttccgagc cctttgttct catcgcctgt
     4081 gggcagggtg atccccggtt ttaccgcgaa gctcaacccg tccttttcat aaaaacgcgt
     4141 cttcaactcc agggatgcat ctccgatgcc acgaacggcg cttttgccgg ctgcatcctc
     4201 ggtcacgacc tcattatagg gaaggcccat tacgatgtcc gtcgcgtcgg tgaggccata
     4261 ggtcaggaag ggaaccgtgg ggacctggag gctcttctcc ctgacgccgt cgtgcttgtc
     4321 aataccgtat tcgccgataa attcgagctg aacattcccc ttcccctgcg tgcccgaatc
     4381 gtctgtgata aggggatgtg cagcaaaagc ctctcccgcc agagcacacg cgattgtaac
     4441 gatagcgacc aacgatgttt tcagcatttc aaactccctc tattcggaat acccatgccc
     4501 ttctggagag aagccggcag aacagctaaa acggcacccc caaaaggaaa cgaacttgac
     4561 gatcatcagt ctttttgttc aagccgtata ctgccccgat gtccgcccat atccggttcc
     4621 ccacccacgc gagcgtgggc ccgacggcgt attgattttc ggaatagctc cccttcgact
     4681 cgacgccgaa cctgaacgac ggcaggactt cataatttat gccggcagcg tattcatgtt
     4741 cctccttgcc cgaggcgtat ctgcgttcaa agacctggtt gtaagccatc ccgactctgc
     4801 cgatttcttt cgccaggatg agcttcccct cgaatctcgg tccgtcttcg tcgttcaagt
     4861 gctcgtcctc cgcgtacaac agcacgtcca cgggcaatgc gtttttctcg gcaacccggt
     4921 acttgagctc caccttgtac ccgaggtacg tcagggaagg ctcgtcggcg gcctgttcat
     4981 aaacctggta cagggcaacg ttgaaacggt ccgtgacgcc gtactccagc tcgacctgtt
     5041 gcgtccagtc gctcgcgttc cgcgtttgga tgtcttccgt cactgcggtg ctccagaact
     5101 cgacctcagc gttgcctttc gccaaagtcc cgtattcttc cgtccagacg aagtttctct
     5161 gcgccgcctg gaccgccccg cccatgaacg taatcaggaa caacaccgcc gcgctgcaga
     5221 atagcttctt cataacgtta acgcctcctt gatgatattg atattcattt tcaacttgac
     5281 cgtacaaaaa aaacgtgttt cccctatctc ttgatattga caactttttc actaggccga
     5341 cttatacatg cccccgtccc atgtcaacaa ttatctttgc gatcgggttc acattttctt
     5401 gacgaagatt tccttcgtaa tgtccttgtc aacggcgatc atggtttctc cgagctggat
     5461 gacgtacgac ggcttcttct gatggagctt gaccatgctc ccgggggaaa gacccatggc
     5521 ggtaaggcgg tccagccgcg aatgcgactc gggtgttatg aacacgatct tggcctgctc
     5581 gcccggcatg aggtcggcc
//
LOCUS       DASSLD010000414         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1110439, whole genome shotgun sequence.
ACCESSION   DASSLD010000414 DASSLD010000000
VERSION     DASSLD010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110439"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFK23_07535"
     CDS             complement(<1..401)
                     /locus_tag="VFK23_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HET7318967.1"
                     /translation="MEQQKPSFIHRVKTLIIGGKHDIEDRNIFHSLSLIAFFAWVGLG
                     ADGLSSSCYGPEEAFLALQGHTYLAIFVAIMSGITVLTIAASYSQLIELFPTGGGGYL
                     VASKLLSPTVGMVSGSALLIDYVLTITISVA"
     gene            complement(566..1732)
                     /locus_tag="VFK23_07540"
     CDS             complement(566..1732)
                     /locus_tag="VFK23_07540"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HET7318968.1"
                     /translation="MIKIVRLVCSVVLAGTAAFAAPAFAQHAKAAGPDPDAVKKALGL
                     SIYVQGGYTYNGNASNVGATQGEENDLRVFDHKANSFTLDLAQIVISRDTTAIDTSGF
                     KVKLSAGEIAKWIHARGLSGAPLDQAQTGQGTDAIDLTEAYVSYLAPIGKGLRFDFGK
                     MVTYFGSEVIEAKDDANYSRSFLFDFAIPFTHTGLKVSYALSAALSASLHVVNGWDNA
                     DDNNAAKCVGFSIGYAPAEAFAGYLNFMTGPERDNNSADKRTLTDLVAIFKPARKVQL
                     ILNADYGTEEKAVAEGTAAWSGMAGIVKYDIDKTYSVAARAEIFNDRDGFRTGTAQTL
                     TELTVTPEIRLAGGLVVRPEYRHDSSNRQSFHRVNGAFTRKSQDTIALGVMYAW"
     gene            complement(1818..>2249)
                     /gene="kdpC"
                     /locus_tag="VFK23_07545"
     CDS             complement(1818..>2249)
                     /gene="kdpC"
                     /locus_tag="VFK23_07545"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HET7318969.1"
                     /translation="GKAVGSKLLGQQFDDPMFFWGRLSATGPYPYNGASSSGSNLGPN
                     NPNLTAAVEARIKALQEVEPGNTAKIPVDMVTASGSGLDPHISLAAAEFQVRRVANAR
                     GLDVTTVRTLVAKQTLGRWLGMYGEPVVNVLELNLTLDGQR"
BASE COUNT          431 a          656 c          674 g          488 t
ORIGIN      
        1 ctggccacgg agatcgtgat ggtgagcacg tagtcgatga ggagcgccga accggacacc
       61 atcccgaccg tgggcgagag cagcttgctg gcgacgagat agcctcctcc gcccgtggga
      121 aagagctcaa tgagctggga gtagcttgca gcgatggtga ggaccgtgat cccggacatg
      181 atggcgacga agatggcgag ataggtatga ccctggagtg caaggaacgc ttcttccggg
      241 ccgtaacagg aagacgagag accgtccgcg cccaacccga cccaggcgaa gaaggcgatg
      301 agggaaagac tgtgaaaaat atttctgtcc tcaatgtcgt gctttccgcc gatgatgagg
      361 gtcttcactc gatgaatgaa tgagggcttt tgctgttcca tccttttacc attctgcctg
      421 ttcgccgtca tgtccgcgcg ccgcgatggt ccgggggccc gtcctttgac gctccgcagg
      481 gcgagacgaa gcagcattat aaatcaagct ctgcgccatt gcaagaacaa gaaatgtgaa
      541 gggggatggg ggtttggaga tgctcctacc aggcgtacat cacgccgagc gcgatcgtat
      601 cctgcgattt cctcgtgaat gcgccgttca cccggtggaa ggactgtctg ttcgaagaat
      661 catgccggta ttcaggccgg acaacgaggc ctccggccag ccggatctcc ggggtcaccg
      721 tgagttcggt cagtgtctgc gccgtgccgg tcctgaatcc gtcccggtcg ttgaagatct
      781 cggccctggc cgcgacgctg tacgtcttgt cgatatcgta cttcacgatg cctgccatgc
      841 cggaccaggc cgcggtgccc tctgcgacgg ctttttcctc tgttccgtag tcggcgttca
      901 ggatcagctg gacctttcgc gccggcttga atatggcgac gaggtcggtc agcgtcctct
      961 tgtcggcgct gttattgtcc cgttcgggcc cggtcatgaa gttgaggtac ccggcgaacg
     1021 cttccgcggg ggcgtagccg atgctgaagc ctacgcactt ggctgcgttg ttgtcgtcgg
     1081 cgttgtccca accgttcacc acgtggaggc tggcactcag ggcagcgctc agtgcgtagc
     1141 tcaccttgag gcctgtgtgc gtgaacggta tggcaaagtc gaacaggaag gatcgcgaat
     1201 agttcgcgtc gtctttcgcc tcgattacct ccgaaccgaa gtaggtcacc atctttccga
     1261 agtcgaaccg cagccccttg ccgatgggcg cgaggtagct gacataggcc tcggtcaggt
     1321 cgatggcgtc ggtaccctga ccggtctggg cctggtccag gggggcgccg ctcaggcccc
     1381 gcgcgtggat ccacttcgca atttctccgg cagagagctt gactttgaag cctgaggtgt
     1441 caatcgccgt cgtatcccgg gagatcacga tctgcgccag atccagggtg aaactgttgg
     1501 ccttgtgatc gaacacccga aggtcgttct cctcgccctg ggtggcgccc acattgctgg
     1561 cgtttccatt gtacgtgtag ccgccctgga cgtaaatgct cagaccaagc gccttcttca
     1621 ccgcgtcagg gtccgggccc gctgcctttg cgtgctgcgc gaatgccggt gccgcaaatg
     1681 ccgccgtgcc tgctagcacg accgagcata caagccgaac gatcttgatc attacctcct
     1741 cccctgtcga caagaatatt ttcctgttcc catgggagtt agtatagcat gaaattctga
     1801 aagaacgaat acgatgatta ccgttgaccg tcgagcgtga gattcaattc gagcacgttg
     1861 accaccggtt caccgtacat tcccagccaa cggcccagag tttgtttggc gacgagcgta
     1921 cggaccgtcg ttacatcgag gccccgcgcg tttgccactc ttctgacctg aaactccgca
     1981 gcagcaaggc tgatatgcgg atcaagccca cttcccgagg ctgtgaccat atccacggga
     2041 atcttcgcgg tattaccagg ttccacctcc tgcagtgctt tgatgcgcgc ttcgacggca
     2101 gccgtaagat tcggattgtt cggcccaagg ttcgatcccg acgatgatgc tccgttatag
     2161 ggatagggac ctgttgccga gagcctgccc cagaaaaaca tcgggtcgtc gaactgctgg
     2221 ccgagaagct tagaccccac ggctttgcc
//
LOCUS       DASSLD010000415         3359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_490472,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000415 DASSLD010000000
VERSION     DASSLD010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490472"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1545
                     /locus_tag="VFK23_07550"
     CDS             238..1545
                     /locus_tag="VFK23_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318970.1"
                     /translation="MFPVLLCLMAFSAAAQNSWAWFDETHIAVAQAAGYRKCYDAAAP
                     DIVKVNLRYTAAKDAGVKNIEEDNHYFDNVGNREVTAAMVLAQAGKYNTTGDVEGHLY
                     GAIIAALRDYRKQRETGYAEEFMVFAAHYIGDLSQPFHNTAGDKAVHIANDGVVEAGV
                     LGNVRKIRDHMYEITLTRTNFEDDLAREIARIANISRALASRVNLRTDRVMTREEAYT
                     QLGHSASLLRAVLYVLDGDNIGPCPVCALSGESGRRTAAGPEAAGASAHEPSLAPVYR
                     IPLRVHLDKSGRKPSDFVEIFDEINEIWLTQAGICFEIEATNGGKTADNGMDLWFYTV
                     LPVSPLTNGYYRSDHEIQVRDTPALAPAAHPARHPAARTAAHELGHGLGLLHRQDLDD
                     NLMRSKTYGWMLNSDEVFAARREAAKKALPDTGPDRCGPPRIP"
     gene            1763..2218
                     /locus_tag="VFK23_07555"
     CDS             1763..2218
                     /locus_tag="VFK23_07555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET7318971.1"
                     /translation="MIVTDLKHISGQCPMTDGMIKAVEFLRLRGIHELPDGEIEIDSV
                     RVYAIVQRYETSKMDVPRFEYHQKYIDVQYIVSGEEIIGWSPAERIKVLEVYDGDKDI
                     CFGAATGEWTPVYVQAGQMAVLYPEDGHAPRLAAGFPSRVMKIVVKVAV"
     gene            2433..2984
                     /locus_tag="VFK23_07560"
     CDS             2433..2984
                     /locus_tag="VFK23_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318972.1"
                     /translation="MEKTGKEKKTVHTGDGAAPEHGKELKPADYLRAVKTHLRDGKQK
                     SAFVLLQQASIQFPDDPFILSYFGYFQAIVDKKYRTGAENCKKAIALLKKSEDIDKEV
                     LYPVFYLNLGRAYVAGGKRKEAIEAFQQGLKFDGGNRELWKDLRGLGERKKPPLPFLD
                     RSNPINKYIGIMTRTGAKTQERK"
     gene            complement(3078..>3359)
                     /locus_tag="VFK23_07565"
     CDS             complement(3078..>3359)
                     /locus_tag="VFK23_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7318973.1"
                     /translation="GEEALAFLQGYADLPSIILLDLKMPGMNGIDTLRTIRSDDRLKH
                     IPVVIVTNSTLESDRSAAFDAGANAFLHKAFDLDQYGRDIQEQLERWAK"
BASE COUNT          791 a          891 c          986 g          691 t
ORIGIN      
        1 tgtccttctg gacattgaag gacatatcgt ccgcggttgc agcaaggagg cggccgaccg
       61 ttcccggctc atgcctggcg ttccacacgg ttggaaacat cgtccgtttc ctgttgacag
      121 ggttgcgtgg taaaggtatc ctgccgcgag aatcattgcg gggtcgtaag cagcaaggaa
      181 agcggagcgg gcaggatgaa ttccggtcag catacagcgg gggtatttaa aaagcgcgtg
      241 ttccccgtac tgctgtgcct catggcattt tcagccgcag ctcagaactc ctgggcgtgg
      301 ttcgacgaga cccatatcgc ggtggcacag gccgcgggat accggaaatg ctacgacgcg
      361 gcagcgccgg acattgtgaa ggtcaacctg agatacacgg cggcgaagga tgccggcgtg
      421 aaaaacatcg aggaagataa tcattacttc gacaatgtcg gcaacaggga agtgaccgca
      481 gccatggtcc tggcgcaggc cgggaaatac aatacaaccg gtgacgtcga agggcatttg
      541 tacggagcca tcatcgcggc cctcagggac taccggaaac agcgggagac cggctacgcg
      601 gaggagttca tggtcttcgc tgcgcactat atcggcgatt tgtcgcagcc gtttcacaac
      661 acggcggggg acaaggccgt gcatatcgcg aatgacgggg tcgtcgaggc cggggtcctc
      721 ggaaatgtcc ggaaaatcag ggaccacatg tatgaaatca ccctgacgcg cacgaatttc
      781 gaagacgatc ttgcccgcga gattgccaga atcgcgaaca tcagcagggc ccttgcatcc
      841 agggtcaacc tgaggacaga ccgggtgatg acgagggaag aggcctacac tcaactggga
      901 cacagcgctt cgcttctccg ggccgtcttg tatgtcctgg atggagacaa tatcggacca
      961 tgcccggtgt gcgcgttgtc cggtgagtcc ggccgtcgca cagcggccgg cccggaagcc
     1021 gccggtgcgt cggcgcatga gccttccctc gcccctgttt acaggatccc gctccgggtt
     1081 cacctggaca agagcggcag gaagccatcg gacttcgtgg agatattcga cgagatcaac
     1141 gagatatggc tgacgcaggc cggcatctgc ttcgagatcg aggcgacgaa cggcggcaag
     1201 accgccgata acggcatgga cctctggttc tacacggtcc ttccagtctc tccgctgacg
     1261 aacggatact accgtagcga ccatgagata caggtgcgtg acacgcccgc cctcgcccct
     1321 gccgcccatc cggcgcggca tcccgcggcg cgcacggccg cccacgagct tggacacggc
     1381 ctggggctgc ttcaccgcca ggacttggat gacaacctca tgcgttcgaa gacctacggc
     1441 tggatgctaa acagcgatga ggtgtttgcc gcccgcaggg aggcggcaaa gaaggccctt
     1501 ccggacactg gaccggaccg gtgcggaccg ccgcggatac catagggtta taataacttt
     1561 gtggacccgt atcttgaatt ttgagtcgag ttatttaagc tgaatcaaaa cctgaaacag
     1621 ttttcgcgca aagacgcaaa gctcgcaaag aaaggccaaa tcattcgggt ttaaaaccaa
     1681 gactaaacga ttccctttgc gttctttgcg gctttgcgcg aggcgctctt gtttccgcat
     1741 tatccggggc aggagtcttt ttatgatcgt tacggacctc aaacatatct ccgggcagtg
     1801 ccctatgaca gacgggatga tcaaggccgt tgagtttctt cgtctgcgcg gtattcacga
     1861 actgccggac ggcgagatcg aaatcgactc tgtccgtgta tacgccattg tccagcgcta
     1921 cgagacatcg aaaatggatg tcccccggtt cgaataccac caaaagtaca tcgatgtcca
     1981 gtatatcgtc tccggcgaag aaatcatcgg ctggtcgccg gcggagcgaa tcaaggtcct
     2041 ggaggtctac gacggggata aggacatctg tttcggcgcg gcgacgggcg aatggacgcc
     2101 ggtctacgtt caggcggggc agatggcggt gctctatccg gaagacggcc acgctcccag
     2161 attggcggca ggatttccgt ctcgcgtcat gaagatcgtg gtcaaggtgg cggtatagcg
     2221 ctgtggcgga acgctccgcc gggagaattc gaaggcaggc tccgtgcaga gtgcggtatg
     2281 aaaagaaatc cgtcattttg acggcccctt ctttaaacga ccaggcgtta agacattcaa
     2341 aataatactg cttcgccaaa atgatttcgt cgaatttcgg agcccttatt tcttttcttg
     2401 tcatcttgca cgattatgtt aaaataggag ttatggaaaa gacgggtaag gaaaagaaaa
     2461 cagttcatac cggagatggc gcagcgccgg agcatggcaa ggaattaaaa ccggcggact
     2521 atcttcgggc cgtaaagaca catctcaggg acggcaagca gaagagcgcc tttgtcctgc
     2581 tgcaacaggc cagtatccag tttccggacg atcccttcat cctctcctat ttcggctatt
     2641 tccaggccat cgtggacaaa aaataccgca ccggcgccga aaactgcaag aaagccattg
     2701 ccttgctgaa gaagagcgag gacatagaca aagaagtgct ctatccggta ttttacctca
     2761 atctcggaag ggcttatgtc gccggcggga agaggaagga agctatcgaa gcttttcaac
     2821 aggggctgaa gtttgatggc ggcaataggg aactctggaa ggacctgcgg gggctgggcg
     2881 agagaaagaa gcccccgttg ccttttctcg accggtcaaa cccgatcaat aaatacatcg
     2941 gcataatgac gcggacgggg gctaaaacgc aggaaaggaa atagataata aagtacaggg
     3001 tataaagtac tgagttcaaa tttcggacga tcattgatgg ttccgacttt gaactttgtg
     3061 ccgtgcaccc tgttttttta tttcgcccac cgttccagct gctcctgtat gtccctcccg
     3121 tactggtcca ggtcgaaggc cttgtggaga aaggcgttcg cgcccgcgtc aaaggccgcg
     3181 ctcctgtcgg attccagcgt cgaatttgtg acaattacca cgggaatatg tttcaaccgg
     3241 tcgtcggagc gtatcgtgcg aagcgtatcg atcccgttca tgccgggcat cttgagatcc
     3301 agaagtatga ttgacggtaa atccgcatag ccctggagaa atgcaagggc ttcctctcc
//
LOCUS       DASSLD010000416         2157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_778027,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000416 DASSLD010000000
VERSION     DASSLD010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778027"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VFK23_07570"
     CDS             <1..916
                     /locus_tag="VFK23_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868933.1"
                     /note="in acetogenic organisms, this enzyme complex
                     converts carbon dioxide to acetyl-CoA while in
                     methanogenic organisms this enzyme is used to degrade
                     acetyl-CoA to form methane and carbon dioxide; part of an
                     enzyme complex; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HET7318974.1"
                     /translation="QNWLRVSINALKEGFVLKHFGDILTTQLKHKFNNIVDKVQVTLY
                     VDDADVKKLLVDAQKSYLDRDVRLASMTDESVDTFYSCILCQSFAPNHVCVVSPERLG
                     LCGAYNWLDAKAAYEIDPNGANQPVQKGETLDVVKGRWKGVDEYVYTNSHQALEYFNA
                     YSIMDAPMTSCGCFECIVAIVPEANGVMVVNRGYTGMTPIGMKFSTLAGTVGGGAQTP
                     GFMGIGRYFLTSKKFLSAEGGFKRIVWMTKNLKESFAEQFKQRAEEEGVPDLLDKIAD
                     ETVAEDSEKMMEFLTAKGHPALTMDPMF"
     gene            1182..1664
                     /locus_tag="VFK23_07575"
     CDS             1182..1664
                     /locus_tag="VFK23_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET7318975.1"
                     /translation="MEEKLEGILVRYQENEGNLISILQDIENEFGYLREDAIYWVSER
                     LDVPPAKFYGIATFYAQFHLKPKGKNVITACCGTACHVKGSERLINSLVKELAIPDGE
                     DTSEDLKFTVEKVNCVGACSIAPVIIINKTVHGKMTPDKLMKETKTLKEGKREYTDYI
                     "
     gene            1753..>2157
                     /locus_tag="VFK23_07580"
     CDS             1753..>2157
                     /locus_tag="VFK23_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET7318976.1"
                     /translation="MNKEVSIKVCMGTGGIAAGGVEVMSSFEKAVAAAGIRATVEKNC
                     SMHKVGCRGFCARDVLVDVIINGDKATYEYIKPDMVERIVSEHILGGTPVADWLVKDD
                     YHTFHDKQVKVVLSDCGEVDPEDIDSYISRGGY"
BASE COUNT          539 a          550 c          622 g          446 t
ORIGIN      
        1 ccagaattgg ctcagggtga gcatcaacgc gctcaaggaa ggctttgtgc tgaagcactt
       61 cggagatatc ctgacgaccc agctcaagca caagttcaat aatattgtcg acaaggtcca
      121 ggtgaccctc tatgtggatg atgccgacgt gaaaaaactg ctcgttgacg cacaaaagtc
      181 ctacctcgat cgcgacgtgc gcctggcaag catgacggac gagtcggtag ataccttcta
      241 ctcctgcatc ctctgccaga gttttgcgcc gaaccatgtg tgcgttgttt cacccgaacg
      301 tctcggcctc tgcggcgcct ataactggct cgatgccaag gcggcctacg agatcgaccc
      361 gaatggtgcg aaccagccgg tccaaaaggg tgaaacactt gacgtcgtca agggtcgctg
      421 gaaaggtgtg gatgaatatg tgtacacgaa ctcgcaccag gcgctcgagt acttcaacgc
      481 ctacagtatt atggacgcac cgatgacctc ctgcgggtgt ttcgagtgta tcgtggccat
      541 cgtgcccgag gccaacggcg tgatggtcgt gaaccgcggt tatacgggca tgacccctat
      601 tggcatgaaa ttctcgacct tggccggcac cgtgggtggc ggcgcgcaga ccccgggctt
      661 tatggggatc ggacggtact tcctcacgag caagaagttc ctctctgcgg aaggcgggtt
      721 caagcgcatt gtctggatga ccaaaaacct taaggagtcc tttgccgagc agttcaagca
      781 gcgcgcagag gaagagggcg tgcctgacct gctcgacaag atcgcagatg aaaccgtggc
      841 tgaagattcg gaaaaaatga tggagtttct gacggctaag ggacacccgg cgcttaccat
      901 ggacccgatg ttttaactgg ctggatgacg ttattttcgt caccctgaac tgagtgaagg
      961 gtttcatgat ttaaaaagac cgggatgctt cttttaggat ggcggtcttt ttcgaagtct
     1021 ggaaacaatt gatcgtgccg gcatgaacca catgccgtga aattgtcgcg caggccgaga
     1081 gcgtgccata cggcacacat cagacccacg accgggacgt tttcaagact gggctgccgt
     1141 gaaagagaag gcggcctttg ttgtctcaag aggatagaaa aatggaagaa aagctcgaag
     1201 gtatactcgt gcgatatcag gaaaacgaag ggaacctgat ctcgatcctg caggacatag
     1261 agaacgaatt cggctatctg cgagaggatg cgatttattg ggtctccgaa aggctcgatg
     1321 tgccgccggc caagttctac ggcatcgcga cgttctacgc ccagttccat ctcaagccca
     1381 agggcaagaa cgtgatcacc gcatgctgcg gcaccgcgtg tcatgtcaag ggctcggagc
     1441 ggctgatcaa cagtttggta aaagaactgg ccattcccga tggcgaggac acatccgaag
     1501 atctcaagtt taccgtggag aaggtcaact gcgtaggcgc ctgcagcatt gcgccagtca
     1561 tcatcatcaa taagaccgtg cacggcaaga tgacacccga taagctcatg aaagagacga
     1621 agacccttaa ggaggggaag cgggagtaca ctgattatat ctaatgctat atgactggga
     1681 aaaccgtttt gcttcttctc gcgcaatgca aagtgaagaa tcaggcctca cgcctcatct
     1741 ttcggagtaa tcatgaataa agaagtctcg ataaaagtct gcatgggtac gggcggaatc
     1801 gccgccggcg gcgtggaagt aatgtcctct ttcgagaagg ccgtcgccgc cgccggcatc
     1861 cgggcgactg tggagaagaa ctgctccatg cacaaagtcg gatgccgggg cttctgcgca
     1921 cgagacgtgc tcgtcgatgt aatcatcaac ggagacaaag caacgtacga gtacatcaag
     1981 cccgacatgg tcgagcggat tgtgagcgag catatcctgg gtggcacgcc cgtagccgac
     2041 tggcttgtga aagacgatta ccataccttc catgacaagc aggtcaaggt agtgctctcg
     2101 gactgcggcg aggtcgaccc tgaggacatc gactcatata tcagccgcgg cggatac
//
LOCUS       DASSLD010000417         2819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1281603, whole genome shotgun sequence.
ACCESSION   DASSLD010000417 DASSLD010000000
VERSION     DASSLD010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281603"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2473
                     /locus_tag="VFK23_07585"
     CDS             <1..2473
                     /locus_tag="VFK23_07585"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET7318977.1"
                     /translation="GIATQNVVERQLAKDHIDRHALGREKFVEKVWEWKKEYGGRIIN
                     QLKRMGASCDWQRERFTMDEGLSRAVREVFVTLYEEGLIYRGERLINWCPRCHTALSD
                     IEVEHEDEKGKLYHIAYPLSQDHNVRLTVATTRPETMLGDTAVAVHPLDPRYKDLIGR
                     TVDLPLTDRKIPIVGDSILVDLEFGTGAVKVTPAHDFNDYEAGMRQRPELPRIKMLDD
                     KAEILADIPDVLPEVQKQITGKPAKKARGMVVELLQARGLLIKTDDHAHSIGKCYRCR
                     SVVEPYLSPEWFVKTGPLAAPAIKVVEDGSVRIIPKGWENTYFEWMRNIKDWCISRQI
                     WWGHRIPAWYCDSCGAVTVSRTDASACSKCGGKQIRQETDVLDTWFSSALWPFSTLGW
                     PDETKELSVYYPTSVLVTSFDIIFFWVARMIMMGLHFRKEVPFKDVYIHALVRDAEGQ
                     KMSKSKGNVIDPIVMIDQYGTDAFRFTLAAFAAQGRDIKMSAERIEGYRNFANKIWNA
                     SRFVMMNLEADFSPNAELITPDSSLADKWILSRLNSVSTEVRSALAEYRFNDAASALY
                     HFIWHEYCDWYLELSKPALGEEKGSASRKAAQTVLARVLETSLRLLHPIMPFITEEIW
                     QTLPESIRNADFGIRNEKQGVKRESIVIAPYPVADETLINPEIERDMQLVMDLILALR
                     NIRGEMNIAPSVQIRAIAKVETREIGGHLEKAASYVRTLARLGELRIGTHETKPKAAA
                     TAGIKGAEIYVPLEGILDLTQARERLQKEIAKISKDIDVFSKKLANRDFVDKAPKAVV
                     EKDTSKLEDFKAKREKLEQSLRMLG"
     gene            2510..>2819
                     /locus_tag="VFK23_07590"
     CDS             2510..>2819
                     /locus_tag="VFK23_07590"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:NF014769.4"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HET7318978.1"
                     /translation="MDASLKELVAHALAEDLGSGDVTSEATIPVDSTSVAVMLAKQHL
                     VLAGLDAAREVFRQLDPSIEFTPFAKDGDAVHGGSEIATVSGNTRALLAGERVALNLL
                     R"
BASE COUNT          614 a          834 c          884 g          487 t
ORIGIN      
        1 gggcatcgcc acgcagaacg tggtagagcg ccagcttgcg aaggaccaca ttgaccgcca
       61 tgccctgggg cgggagaagt tcgtcgagaa ggtctgggag tggaagaagg aatacggcgg
      121 caggatcatc aatcagctca agcgcatggg ggcgtcctgc gactggcagc gcgagcgctt
      181 caccatggac gaagggctct ctcgcgccgt gcgcgaggtc tttgtcaccc tgtacgagga
      241 agggctcatt taccgcggcg agcgtctcat caactggtgc ccccgctgtc acacggcgct
      301 ctcggatatc gaagtggagc acgaggacga gaagggcaag ctctaccata tcgcctaccc
      361 cctctcccag gatcacaacg tccggcttac ggtggcgacg acccgtcccg agaccatgct
      421 cggagacacg gcggtagccg tgcatccgct cgatccgcgc tacaaagacc tgatcggcag
      481 gaccgtcgac ctgccgctca cagacagaaa gatcccgatc gtcggcgact ccatcctcgt
      541 tgacctggaa ttcggcaccg gcgcggtcaa ggtgacgcct gcccatgatt tcaacgacta
      601 cgaggcggga atgcgccaaa ggcctgagct gccgcggatc aagatgctcg acgacaaggc
      661 cgagatcctc gccgacatcc ccgacgtgct tcccgaggtg cagaaacaga tcacgggcaa
      721 gccggcaaag aaggcgcgcg gcatggtcgt ggaactcctg caggcgcggg gcctgctcat
      781 caaaacggac gaccacgccc attccatcgg caagtgctac cgctgccggt ccgtggtcga
      841 gccctacctt tctcccgagt ggttcgtgaa aaccggcccg cttgccgctc ccgcgatcaa
      901 ggtcgtcgaa gacggcagcg tgcggatcat tcccaagggc tgggaaaaca cctatttcga
      961 atggatgagg aacatcaagg actggtgcat ctcgcgccag atctggtggg gccatcgcat
     1021 cccggcctgg tattgcgatt cctgcggggc ggtcacggtc agccggacgg atgcatcggc
     1081 atgctcaaag tgcggcggca aacagatccg gcaggagacc gacgtgctcg acacgtggtt
     1141 ctcgtcggcc ctctggccct tttccacgct cggatggccc gatgagacga aggagctttc
     1201 cgtctactat ccgacctcgg tcctcgtcac gagcttcgac atcatctttt tctgggttgc
     1261 acgcatgatc atgatgggcc tgcatttcag gaaggaggtg ccgttcaaag acgtctacat
     1321 ccacgcgctc gtgcgcgatg ccgaaggcca gaagatgagc aagtccaagg ggaacgtcat
     1381 cgaccccatc gtgatgatcg accagtacgg caccgacgca ttccggttca cgctcgccgc
     1441 gttcgcggcc caggggaggg acatcaagat gtccgcggag cgcatcgagg gctaccgcaa
     1501 cttcgcgaac aagatctgga acgcgagcag gttcgtgatg atgaacctcg aggcggattt
     1561 ttctccgaat gccgaactta tcactcccga ctcgtcgctc gccgacaagt ggatcctctc
     1621 ccgcctgaac tcggtcagca cagaggtgcg atccgcgctc gcggaatacc ggttcaacga
     1681 cgcggcctcg gccctgtacc acttcatctg gcacgagtac tgcgactggt atctcgaact
     1741 ctccaagccc gcgctcggtg aagaaaaggg atcggcgtcg cggaaagcgg cgcagaccgt
     1801 gcttgcccgc gtcctggaaa cctcgctccg gctcctgcac ccgatcatgc cgttcatcac
     1861 cgaggagatc tggcagacgc tgcctgaatc aattcggaat gcggatttcg gaattcggaa
     1921 tgaaaagcaa ggcgtaaagc gcgaatcgat cgtgattgcg ccctatcccg tggcggatga
     1981 aacactgatc aacccggaga tcgagcggga catgcagctt gtgatggatt tgatccttgc
     2041 cctccggaac attaggggcg agatgaacat cgcgccgtcg gtgcagatac gggcgatcgc
     2101 gaaggtggaa acaagggaga tcggcggaca cctggaaaaa gcggcgagct acgtcaggac
     2161 cctcgcgcgc ctgggcgagc tccggatcgg tacgcacgag accaagccca aggccgctgc
     2221 aacagcggga atcaaggggg cggagatcta tgtgccgctc gagggaatcc tcgatctcac
     2281 gcaggcgcgg gagcggcttc agaaggagat tgcgaagata tcgaaggaca tcgacgtctt
     2341 ctcaaagaag ctcgcgaaca gggacttcgt ggacaaggcg ccgaaggcgg tggtggagaa
     2401 ggacacgtcc aagcttgaag acttcaaggc gaaacgagag aagctggagc agagcctgag
     2461 gatgttgggg taagataatc gacctgcgac agaggagatt gatgacaaca tggacgcttc
     2521 gttaaaagag ctggttgctc acgcccttgc cgaggatctc ggctcgggag acgtgacctc
     2581 cgaggcgacg atcccggtcg actcaacttc cgttgccgtg atgctggcga aacagcacct
     2641 ggtgcttgcg gggctagacg cggcccggga ggtgtttcga cagctcgatc cgtcaataga
     2701 gtttaccccc tttgccaaag acggagacgc cgttcacggg gggagcgaaa tcgcgacggt
     2761 atccgggaac acgcgggcgc tcctggcggg cgagcgcgta gcattgaacc tgctccgcc
//
LOCUS       DASSLD010000418         4400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1398786, whole genome shotgun sequence.
ACCESSION   DASSLD010000418 DASSLD010000000
VERSION     DASSLD010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398786"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /gene="cysK"
                     /locus_tag="VFK23_07595"
     CDS             complement(<1..851)
                     /gene="cysK"
                     /locus_tag="VFK23_07595"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET7318979.1"
                     /translation="MDKIYDDITKTIGRTPLVRINRMARGLKTEVAAKLEKANPLASV
                     KDRIGLAMIEAAEQAGLINKDSVIIEPTSGNTGIALAYVCAVKGYRLILTMPETMSRE
                     RRALLRIFGAELVLTEGAKGMKGAIEKAEELAREQRGTFIPQQFRNAANVDIHRKTTA
                     EEIWDDTEGRIDILVSGVGTGGTITGVAGVIKKRKPSFQTIAVEPKDSPVLSGGKPGP
                     HKIQGIGAGFVPDILQRELIDEVIQVSNDDAGATARRAAREEGLLVGISSGAALWAAL
                     EAARRPE"
     gene            complement(844..1332)
                     /locus_tag="VFK23_07600"
     CDS             complement(844..1332)
                     /locus_tag="VFK23_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7318980.1"
                     /translation="MKVLVAVDESPDAQAAVRYGIQKVCELGGELITLHVFPKYLFDG
                     HDAGQKAEQISRRESLDLLEGALKVIRENGRNIRTRLVMTDGRPEETIFEYAKEEDVD
                     LIVSPPRFGSLIEKVCCLMDIVSAEEQAVSEGPVACIPPEALAAAWTRRCPPGVTGER
                     HG"
     gene            complement(1373..2515)
                     /locus_tag="VFK23_07605"
     CDS             complement(1373..2515)
                     /locus_tag="VFK23_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HET7318981.1"
                     /translation="MGETTAVLANVDELKRVGIVGQKQKDYYLIRLRAIGGGMTAGEL
                     DVVAAVSRKYGDGKVHLTTRQGVEIHNIHADDLRAAREELELGGIVLGVCGPRGRGIV
                     ACPGAATCTSGIIETKELAAELDAAYLRKQAPHKFKIGISGCPNNCSKPVENDIGVMG
                     GVLPGWNREACIACKLCVSICPAQAIEQRDKDFVLDAEKCILCGLCINNCPSSAWTAQ
                     KTGYTLYLGGTMGKKPRLGTKARVLIETKEELKGYINRAFDYYVAHGKKKERFGHTLD
                     RIGVDKVFAEIFSEEKKSAPAAAEHDSPSAARNNFLDLRGVCCPLNFVKAKLAMDKIK
                     SGETIEFYLDDGEPIVNVTRSLKDEGHQVLLVLPQENYFKVLAEKG"
     gene            complement(2915..3304)
                     /locus_tag="VFK23_07610"
     CDS             complement(2915..3304)
                     /locus_tag="VFK23_07610"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HET7318982.1"
                     /translation="MDEPVKKQRKKIAVQLSSGINDVGKVKSAIMFATIAQCSGCDAV
                     VYCVQDGADAVIKGKIKEEGLLPPGVPTFEQRLADALQVGVKFQLCEQVAKNRGLTNG
                     DLIDGTQIAGGIHLITYALEYDGMLYF"
     gene            3489..4352
                     /locus_tag="VFK23_07615"
     CDS             3489..4352
                     /locus_tag="VFK23_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HET7318983.1"
                     /translation="MRTVMLLTKDGSLKTEVSALLPKGYKLVAGEEKKALPADLACIV
                     FDIDTLEVHRIKEYGEKSFVLAVTDQEQTGPVMEAATWGAYEIMHRPLRKERIARILE
                     ELHELRREMTGVVPISKDVLAPAATCVIIGRSSLVMGLCEKVARIAQVEVPVLITGET
                     GTGKELVAEAIAQLSSRFGKPFVVINSAAVPDTLLESELFGYERGAFTGAVVAKEGML
                     KAADGGCVFFDEIGEMPLQLQGKLLRFLQTQTFYPVGSTKEVQVNVRVISATNRDLAE
                     MVREGKFRGDL"
BASE COUNT          847 a         1304 c         1215 g         1034 t
ORIGIN      
        1 ttctccggcc tccttgcagc ctcgagcgcc gcccagagcg ctgcgccgga ggatatcccg
       61 acaagcaggc cttcctccct cgcagccctt ctcgccgtgg cgcctgcgtc atcattggac
      121 acctggatga cctcgtcgat cagttcgcgc tgcaggatat ccggaacgaa tccggcgccg
      181 atgccctgga ttttgtgggg tccgggcttt cctcccgaaa gaaccggaga atccttcggt
      241 tccacggcaa tggtctgaaa cgagggtttt ctcttcttga taacacctgc gacgccggtg
      301 atggtcccgc cagtgcctac gcctgaaacg aggatatcaa ttctcccttc cgtatcatcc
      361 catatctctt ccgccgtcgt ctttctgtga atgtccacat tcgcggcatt cctgaactgc
      421 tggggaatga acgttcctct ttgttcccgt gcgagctctt ccgctttttc gatagcgcct
      481 ttcatgcctt tcgcgccctc ggtcaggaca agttccgcgc cgaaaatcct caggagcgca
      541 cggcgctccc tactcatggt ttcgggcatg gtgaggatta accggtaacc cttgaccgca
      601 cagacgtacg cgagcgcgat gccggtgttc ccgctggtgg gctcgatgat gaccgagtct
      661 ttgttgatga gccctgcctg ttccgccgcc tcgatcattg ccaggccgat ccggtccttt
      721 acgctggcta aaggattagc cttctcgagc ttcgccgcaa cctcggtctt gagcccccgc
      781 gccatcctgt tgatcctgac caggggagtc ctgccgatgg ttttcgttat gtcatcgtaa
      841 attttatcca tgccgctccc ctgttactcc gggagggcag cgccttgtcc atgccgccgc
      901 gagagcttcg ggcggaatgc atgcaacagg cccttcggag accgcctgct cctccgcgga
      961 gacgatgtcc atgagacagc atactttctc gatcagggag ccaaaccgcg gaggagaaac
     1021 aatcaagtcc acatcctctt cttttgcata ctcaaatatc gtctcctcgg gacggccgtc
     1081 ggtcatgacc agccgtgtac ggatgttcct gccgttctct cggatcacct tcaatgcccc
     1141 ttcaagaagg tccagagatt ccctccggga gatttgctct gctttctgcc ccgcgtcatg
     1201 accgtcaaaa aggtattttg gaaatacatg gagggtgatc aattcccctc cgagttcaca
     1261 aactttttga attccgtatc tcacggcagc ctgagcatcg ggcgattcat caactgcgac
     1321 caataccttc atagatatct cctcttttca cgatgatccg gagacgtggc tttcagccct
     1381 tttccgccag gaccttgaag tagttctcct gggggagcac caggagcacc tgatgcccct
     1441 cgtccttgag gctccgcgtg acgttcacga tgggctcccc gtcgtcgaga tagaactcga
     1501 tcgtttcgcc gctcttgatc ttgtccatcg cgagcttggc cttgacgaag ttaagcgggc
     1561 agcagacgcc ccggaggtcg agaaagttat ttcttgccgc ggacggggag tcatgttctg
     1621 cggcggcagg agcgctcttt ttctcctcgg agaaaatctc ggcaaagacc ttgtccactc
     1681 cgatccggtc gagcgtgtga ccgaagcgtt ccttcttctt tccgtgggcg acataataat
     1741 cgaaggcccg gttgatgtat cctttcaatt cctcctttgt ctcgatcaac acccttgcct
     1801 tcgttcccag ccttggcttc tttcccatcg tcccgccgag atacagggtg tacccggtct
     1861 tttgtgcggt ccaggctgac gacgggcagt tgttgatgca gagcccgcag aggatgcatt
     1921 tttcggcgtc gaggacgaag tccttgtccc gctgctcgat cgcctgggcc gggcagatgc
     1981 tgacgcagag tttgcaggcg atgcaggcct ccctgttcca gcccggcaga acgccgccca
     2041 tgacgccgat gtcgttttcg acgggctttg agcagttgtt cgggcagccg gagatcccga
     2101 tcttgaactt gtgcggcgcc tgcttgcgca aatatgccgc gtcaagctct gcggcaagct
     2161 ccttggtctc gatgatgccc gatgtgcagg tcgccgctcc cggacaggcg acgatgcccc
     2221 ttccccgggg gccgcacacg ccgagcacga taccccccag ctcgagttct tccctcgcag
     2281 cccggagatc gtccgcatgg atgttgtgga tttcgacgcc ctggcgcgtg gtaagatgca
     2341 ccttcccgtc gccgtacttc cgggacactg cggccacgac gtccagttcc ccggcggtca
     2401 tccctccgcc gatagcccga aggcggatga gatagtagtc tttctgcttt tgtcccacga
     2461 tgccgacccg cttgagttca tcgacgttcg ccagcactgc cgttgtttct cccatgcttt
     2521 cacctcgtat gagtttgatc gtttctgcaa tgcgaaggcg cggagtaaga gggggaggac
     2581 gaatttgctc cgcgccttcg cgtttgcaaa cttcatgaca gctaaagcaa catgcatgcc
     2641 aataaataaa aaacgtataa tcagacggtt tccctccggc atggatcgcc gctggttctt
     2701 atttatacag tctgtatgtt atttatactc ttgaagtgct tggattggca cggcggtctc
     2761 tccgcaggcg gacgcacgga acggcaatag gttccgatga gaaattgctt tgcgggggga
     2821 cgggcgccga atcagacggg ggagggaaat tctcgaagca ccgggcgtga accggcttta
     2881 cgtcagtgaa attgattgcc ggcagcgaag gcttttaaaa atagagcatc ccatcatatt
     2941 ccagcgcgta cgtgatcagg tgtataccgc cggcgatctg agtcccgtcg atcaggtcac
     3001 cgttggtaag gccacggttc ttcgcaacct gttcgcacaa ctggaacttg acgcctactt
     3061 ggagggcatc ggcgaggcgc tgctcgaatg tcggcacacc cggcggcaat agtccctctt
     3121 cttttatctt ccctttgatg acagcgtccg ccccgtcctg gacacagtag acaacggcgt
     3181 cgcagccgga gcactgggca atggtggcga acatgatcgc ggacttcacc ttaccaacgt
     3241 cgttgatccc cgacgacaat tgcacggcaa ttttttttct ctgtttttta acgggttcat
     3301 ccatgttgtt ctccttagct tattgtgatg cttccataag gcggtttaat ccgttttgcc
     3361 tgccgcatat ccagcaatta ccataccacg tctgcttgag ataaaataaa gctttttcgg
     3421 gaaccggcga ataggtgttg atttgccaat atacaccttg tatattattt aaacatgtgg
     3481 aggctcccat gagaacggtt atgctgttga cgaaagacgg ttccctgaaa acggaagtat
     3541 cggcccttct gccgaaagga tacaaactcg tcgcaggaga ggaaaagaag gcgctgccgg
     3601 cagatcttgc ctgtattgtg ttcgatatcg acacgctcga ggtccaccgg ataaaggagt
     3661 acggcgaaaa gagtttcgtc cttgcggtaa cggaccagga gcagaccggt ccggtcatgg
     3721 aagcggcgac ctggggggcc tatgagatca tgcaccgccc cctcaggaag gaacggattg
     3781 cacgcatcct cgaggagctg catgagctca gacgggagat gacgggcgtc gtgccgatct
     3841 ccaaggacgt tctcgccccc gcggccacct gcgtgatcat cggcaggtcg tccctcgtca
     3901 tgggactctg cgaaaaggtg gcccgcatcg cgcaggtcga ggtcccggtc ctgattaccg
     3961 gcgagacggg cacgggaaag gagctcgttg ccgaggccat tgcccagcta tcgtcccggt
     4021 tcggcaagcc cttcgtggtc atcaattccg cggctgtgcc cgacaccctc cttgaatcgg
     4081 aactcttcgg gtacgaacgg ggtgccttca cgggagccgt cgttgcaaag gaaggaatgc
     4141 tcaaagcggc ggacgggggc tgcgtgttct tcgacgagat cggagaaatg ccgctgcagc
     4201 tccagggaaa gctccttcgc ttccttcaaa cgcagacctt ttatcccgtg ggaagcacga
     4261 aagaggtcca ggtgaacgtt cgcgtgatct cggccacgaa ccgagacctc gcggaaatgg
     4321 tacgcgaggg gaaattccgg ggagacctgt gacaccggct tcgggtcgcc tccattcacg
     4381 tccctccgct cagggaccgg
//
LOCUS       DASSLD010000419         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1037987, whole genome shotgun sequence.
ACCESSION   DASSLD010000419 DASSLD010000000
VERSION     DASSLD010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037987"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFK23_07620"
     CDS             <1..291
                     /locus_tag="VFK23_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008043568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7318984.1"
                     /translation="GTLSVSSSRGLDDRGHDAVMVSFADSGTGIPPAEIQKIFDPFYT
                     TKEAGKGTGLGLSLSYDIVKRFKGDIHVESEVGKGTVFTILLPIEARETVTS"
     gene            409..1767
                     /locus_tag="VFK23_07625"
     CDS             409..1767
                     /locus_tag="VFK23_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7318985.1"
                     /translation="MEKANILIVDDEKDICMALNMLLTKEGYTVKEAYNGEQAMERIK
                     QENFDVVMTDIKMEKMDGFEVLKQVQKVSPETSVILMTAFATVGSAVEAMRAGAADYI
                     TKPFLNDEIRLTVKRLLQSRDLRMENEILRQELSQRREAFVNIIGNSEPMQKVFTVME
                     KVIPNKSNILITGESGTGKGLVAQAIHDAGPRKDKPFISINCGAIPENLLESELFGHK
                     KGAFTSANEDKKGLITMANGGTLFLDEIGELPPALQVKLLHIIQTKELTPVGDTRVIT
                     VDVRIIAATNADLMQRVKENRFREDLYYRLNVIEIRMPSLRERRDDIPLLIKHYLGIA
                     AKEAEKNVKDIDYEAMQSLLAYDWPGNIRELRNTIERATVLADGEMITIHDLPDKFRT
                     LDIEGVSTSSLRQALDEFEREYIRRSLADNKFNKEAAAGKLGIDLATLYRKLKKLRIE
                     TA"
     gene            complement(1770..1988)
                     /locus_tag="VFK23_07630"
     CDS             complement(1770..1988)
                     /locus_tag="VFK23_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318986.1"
                     /translation="MQDIQSMLGKEVEVIANGVRYTGVLIEVSDVEVHIKGPMQWIAL
                     PVSAVSEVKLKTDAAPRPDRFSDWGTGQ"
BASE COUNT          505 a          610 c          581 g          400 t
ORIGIN      
        1 gggacgctgt ccgtcagctc gagccggggt cttgacgacc gcggccatga tgccgtcatg
       61 gtctccttcg ccgacagcgg aacgggaata cctccggctg agatccagaa gatcttcgac
      121 cccttctaca cgaccaagga agccggcaag ggtaccggac tgggattgtc cctgagctac
      181 gacatcgtaa agcgttttaa aggcgatatc cacgtggaaa gcgaagtcgg aaagggcacg
      241 gtcttcacga tcctgctgcc gatcgaagcg cgggagaccg taacaagttg aatataatgt
      301 catttccgaa ggctttaatc gggaatgttg ccttctaatc tggaagaaac accggatccc
      361 ccgatagaac cttcggggat gacagactat ttggagtaag gagcacccat ggaaaaagca
      421 aacatcctga tcgtcgacga cgagaaagac atctgcatgg cgttgaacat gctgctgacc
      481 aaggaagggt ataccgtcaa ggaggcatac aacggcgagc aggccatgga gcggatcaag
      541 caggaaaatt tcgacgtcgt catgaccgac atcaagatgg agaaaatgga cggtttcgaa
      601 gttctgaagc aggtgcagaa ggtcagcccc gaaacatcgg tcattctgat gaccgccttt
      661 gctacggtgg ggtcggctgt cgaggcgatg cgggccggag cggccgatta cattacgaag
      721 ccgttcctca acgatgaaat caggctgacc gtcaagcgtc ttctccagag ccgggacctc
      781 cggatggaaa acgagatcct gcgccaggag ttgagccagc ggcgcgaggc attcgtcaac
      841 atcatcggca actcggagcc catgcagaag gtctttacgg tcatggagaa ggtcatcccg
      901 aacaagagca acatcctgat caccggcgaa agcggtaccg gaaaggggct cgtggcacag
      961 gcaatccatg atgcggggcc gcgcaaggac aagccgttca tctccatcaa ttgcggggcc
     1021 atcccggaaa acctgctgga aagcgaactc ttcgggcaca agaaaggcgc tttcacctcc
     1081 gcgaacgagg acaagaaggg cctcatcacg atggcgaacg gcggcaccct gttcctggac
     1141 gagatcggcg aactgcctcc ggcgctgcag gtaaagctcc tccacatcat ccagacgaag
     1201 gagctcactc ccgtgggcga cacacgcgtc attaccgtgg atgtccggat tatcgccgca
     1261 acgaacgccg acctcatgca acgggtaaaa gagaaccggt tccgcgaaga cctctattac
     1321 cgcctgaatg tgatcgagat ccgcatgccc tcccttcgtg aacggcgaga cgacatcccg
     1381 cttttgatca agcactatct gggcatcgct gcgaaggagg ccgagaagaa cgtcaaggac
     1441 attgactatg aggccatgca gtccctgctc gcctatgact ggccgggaaa tatccgggaa
     1501 ctccggaaca ccatcgagcg cgccaccgtc ctggccgacg gtgagatgat caccatccat
     1561 gacctgccgg acaagttcag aaccctggac atcgagggcg tctcgacgtc atcgctccgc
     1621 caggcgctcg atgaattcga gcgggagtat atccggcgca gcctggcgga caacaagttc
     1681 aacaaggaag cggcggcggg aaagctcggg atcgatcttg ccaccctgta ccggaagctg
     1741 aaaaagctga ggatcgagac ggcgtaatgc tactgtcccg tcccccagtc actgaaccgg
     1801 tcgggacgcg gagctgcgtc ggtcttgagt tttacctcgc tcaccgccga cacgggaagc
     1861 gctatccact gcatgggccc ctttatgtgc acttcaacgt ccgagacttc gatcaatacg
     1921 ccggtatacc gtaccccgtt cgcgatcact tccacttcct tcccgagcat cgattgaatg
     1981 tcttgcatta tccctcctcc ttcgttctcg tgactcctgt ctcctgactc ctgactcctt
     2041 actgctgttt catcatattt ccagtttccc ccagctctcc ttccgataac gccata
//
LOCUS       DASSLD010000420         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_982028,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000420 DASSLD010000000
VERSION     DASSLD010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982028"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFK23_07635"
     CDS             <1..193
                     /locus_tag="VFK23_07635"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680565.1"
                     /note="Converts isocitrate to alpha ketoglutarate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HET7318987.1"
                     /translation="AIARRGELDGAPTVVAFAKRLEEVVIRTVEDGVMTKDLASIAEP
                     RPAQHALTEEFLDAVAKRL"
     gene            451..642
                     /locus_tag="VFK23_07640"
     CDS             451..642
                     /locus_tag="VFK23_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013603780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HET7318988.1"
                     /translation="MPAAKKKANCSVCGTEVKQDSKFKADYKGQTHYFCSSKDKKEFE
                     DRPHVYAGILRVSKTGRAA"
     gene            complement(768..1796)
                     /locus_tag="VFK23_07645"
     CDS             complement(768..1796)
                     /locus_tag="VFK23_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318989.1"
                     /translation="MGKNRAPYGSMNHPREFSIALVIVMVLALDLLTACDILSGKAGE
                     KPSRTPLDVPSVLAGSKPVFVSDYFSFVGSDERGHVAFAIDNDRSRRGNKFSADAHVF
                     LHDERTGWVTVSGNGGYENRRGELLRIPDSPDFQFIGEVKDGITLVSPRNRLTLSIDP
                     MQERFSRATGDALFSMGSTSATLAWGDRVIPGRVIYEYLFVLNLSPWYSYLSGLFYND
                     FQGLYLETGDGGDFYLRSEKKTGWSRSFGNALGFQVLDRKSEVLEDLRISVPERSLAV
                     GFYRWPRAWQASWRGSRGRGSLTVKVVDRKNVTNWFIGGFAMAIVEGELSYDGSVRPV
                     YGLAELIR"
     gene            2142..>2546
                     /locus_tag="VFK23_07650"
     CDS             2142..>2546
                     /locus_tag="VFK23_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET7318990.1"
                     /translation="MAFIDNDVTADVMDVQPEEEIRICLFSIGIDTYAIPVDVLTEIV
                     IPQKIFPVPTTPSHVLGVINLRGNIVPVVDIRPALSLPQQSVPGQIAILKLGAMMLGI
                     VVDNVSAVIPVPESSIRTLPDDAAEKQTANANR"
BASE COUNT          592 a          734 c          710 g          510 t
ORIGIN      
        1 cgcgattgcc cggcggggcg aactggacgg cgcgccgacg gtggtcgcct tcgcgaagag
       61 gcttgaagaa gtcgtgatca ggaccgtcga ggacggcgtc atgaccaagg acctcgcttc
      121 cattgcggag cccagacccg cacagcacgc ccttaccgag gagtttctgg acgcggtggc
      181 aaagagatta taacgcggag atgtttgccg atgaaccaga aacgtcagcc cagcgggcat
      241 actgatcgag gtcggtcggc tgctcgcagc acaccggggc aaaaagtatt aagtttttgt
      301 ttattccccc cttttctctt caattttttc ccggtcgtga tccttccgtt tgatgcatcc
      361 ctatgaaaaa ccggactaaa tacaccttca tttgacactt cgccagacat gttgctatag
      421 taataaatgg ggggagggag gaggatgtac atgccggcag caaaaaagaa agcgaactgt
      481 tccgtgtgcg gcacggaagt aaagcaggat tcaaagttca aggccgatta caagggacag
      541 acacattatt tctgcagcag caaagacaag aaggagttcg aggacaggcc ccacgtatat
      601 gccgggatat tgcgggtaag taaaaccggc agggcagctt gaaggaatcc caagctgcat
      661 cgtgacttcg gtgtgttgta gaggtccggg gagcgatgac gcctcccgga ctttttttca
      721 tgatcctgct cgtcttccgt taacatacct cggagcaggc ggcagaccta ccgtatcagt
      781 tctgccaagc cgtagaccgg acggacgctc ccgtcgtaag acagttcacc ctcgacgata
      841 gccatggcga acccgccgat aaaccagttg gtcacattct tccggtcgac aacctttacg
      901 gtaagcgagc ctcggccccg gcttccccgc caggacgcct gccaggccct gggccatcgg
      961 tagaagccga cggcaaggct tcgctcagga acgctgatcc gcaggtcttc caggacctcg
     1021 ctcttgcgat caagcacctg gaaccccagc gcgtttccga acgaccgaga ccatccggtc
     1081 tttttttcac ttctcagata gaagtcgccg ccgtcgcccg tttccagata cagcccctgg
     1141 aagtcgttgt agaacagccc ggaaagatac gagtaccagg gcgacagatt aagcacgaag
     1201 aggtattcgt agatgacccg tccggggatc acccggtccc cccaggcaag cgtagccgat
     1261 gtcgagccca tcgaaaaaag agcgtcaccc gtggcgcgcg agaaccgctc ctgcatcgga
     1321 tcgatggaaa gcgtaagacg gttccggggg ctcacgaggg ttattccgtc ttttacctct
     1381 ccgatgaatt gaaagtccgg agagtcgggg atgcgcagca gttcccccct tctgttctcg
     1441 tatccgccgt tacccgagac cgtgacccat cctgtgcgct catcgtgcag gaacacatgg
     1501 gcgtcggcgc tgaatttgtt cccacggcga gaccggtcat tgtcgatcgc gaaggcgaca
     1561 tgacctcgtt catccgagcc gacgaaagaa aagtagtcgg aaacgaaaac aggcttcgac
     1621 cccgcaagca ccgacggtac atcgagaggc gtgcgcgatg gtttttcacc cgccttgcct
     1681 gagaggatgt cgcaggcggt gagaaggtcc agtgccagaa ccatcacgat gaccaaggcg
     1741 atgctgaatt ctcggggatg gttcatactg ccgtaggggg ctcgattctt tcccatgatc
     1801 actccatcac gctgccgtcg tttaaagagt agcgcctccc gcgacggaat gtcaacccat
     1861 cgcaagcacc ctacaactga aataggaaag cttgacaccc cccacagcaa tatgttattt
     1921 tttaccgact tgccgactcg ttgaccgggt ccgcagagat attaaaaaca acgacaagtt
     1981 caagtacctc ccggttatca tggccacgtc cagagaccgg gaaagcgaca cgttctgggg
     2041 gatgaagccg ggggagggtg aatatttgac caaaccgttc aggccggaga tacgctgaaa
     2101 gcggtcgaaa aaatgcatcc agtccctgga gggggccccg tttggccttt attgacaacg
     2161 acgtcaccgc cgatgtgatg gatgtccagc cggaagagga gattcggatt tgtctcttca
     2221 gcatagggat agacacctac gccattcccg tcgacgtgtt gacggaaatc gtcatcccgc
     2281 agaaaatttt ccctgttccg accaccccgt cccacgttct cggtgtcatc aacctccggg
     2341 gcaacatcgt tccggtcgtt gatattcgcc cggcgctttc cctgccgcag cagtccgttc
     2401 ccggacagat cgccatcctc aaactcggtg cgatgatgct cggtatcgtc gtcgacaacg
     2461 tctcggcagt gatcccggtg ccggaaagca gcatccggac gctgccggac gacgcggcgg
     2521 agaagcagac cgcaaacgcg aaccgg
//
LOCUS       DASSLD010000421         2453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_849736,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000421 DASSLD010000000
VERSION     DASSLD010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849736"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VFK23_07655"
     CDS             <1..1128
                     /locus_tag="VFK23_07655"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997080.1"
                     /note="catalyzes the reduction of adenosine
                     5'-phosphosulfate to AMP and sulfite; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylylsulfate reductase subunit alpha"
                     /protein_id="HET7318991.1"
                     /translation="TQMENRIVLTRFKDGYGPVGAYFLHLKTYTQNAYGQDYEAFWRD
                     SIEKLVGDYVNRHPVPTCLRNHALLEEVKAGRGPIRMVTKEAFQDPHKEKVGWENFLG
                     MTIGQAVVWASQNIDPKEINPELTTSEPYVMGSHATCSGAWASGPEDFAPAEYQWGYN
                     RMMTVEGLFGAGDTIGGTAHKFSSGSFTEGRIAAKAAVKYVNDLGQQQPQVSEAEYEE
                     LKKTVYKPLETYAVGRNEIVAGTVSPSYLLPIHGLQRLEKIMDEYVGGIGAHYTTNEP
                     LLTRGIELLIMLKEDLAYLGAEDLHQLQRAWELQHRVLASESVTQHTLFRTETRWPGY
                     YYRADHPKLDDKDWHCFTLSRYDRYSGKWEMEKAPVYHIID"
     gene            1266..>2453
                     /gene="sat"
                     /locus_tag="VFK23_07660"
     CDS             1266..>2453
                     /gene="sat"
                     /locus_tag="VFK23_07660"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014995887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HET7318992.1"
                     /translation="MSQLVPPHNSEQVKPLLLPETERAEERKRAEKLRKIPLDSRSVS
                     DVFMLAMGAYTPLDGFMGHDDWRGSCLDMKLTNGVFWPIPITLPVNKDLADSIHTEEE
                     VTLIDAETGEILAVMDVHEKYSIDKQLEAEHVYRTADPKHPGVAKVLDQGDVNLAGPL
                     MVLSEGEYPAKYPDLYIRPAESRAMFLERGWSNVAAFQTRNPMHRSHEHLVKIAVEVS
                     DGVFIHQVLGKLKAGDIPADVRTRAIQAMIDNYFRPGTVIQAGYPIEMRYAGPREALL
                     HALIRQNFGCSHLIVGRDHAGVGDYYGPFDAHHIFDQLWDGALYTKPLKIDITFYCRK
                     CYGMATAKTCPHDPKHHISISGTQQREMLSTDADIPPEFSRPEVVAILKEYYAGLRSA
                     GLGR"
BASE COUNT          548 a          716 c          714 g          475 t
ORIGIN      
        1 actcagatgg agaaccggat cgtcctcacc cgattcaagg acggctatgg tccggtcggc
       61 gcctacttcc tgcatctcaa gacctacacg caaaacgcct atgggcagga ttacgaggcc
      121 ttttggcgcg attctatcga gaagttggtc ggcgattacg ttaatcggca cccggtgccg
      181 acgtgcttgc ggaaccacgc cctcctcgag gaggtcaagg ccggcagggg cccgatccgc
      241 atggtgacga aggaggcctt ccaggatccg cacaaggaga aggtcggctg ggagaacttc
      301 ctcggcatga cgatcgggca ggcggtggtc tgggcgtcgc aaaacattga tcccaaggaa
      361 ataaaccctg aactgaccac gtcggagcct tacgtcatgg gctcccacgc gacgtgttca
      421 ggcgcctggg cgagcggccc ggaggacttt gcacccgcgg aatatcagtg gggctacaac
      481 cgaatgatga ccgtcgaggg cctcttcggc gccggcgaca ccatcggcgg caccgcccat
      541 aagttctcgt cgggatcttt cacggaaggc cgcatcgccg ccaaggcggc ggtcaaatac
      601 gtgaatgacc taggtcaaca gcagcctcag gtcagtgaag ctgagtacga agaactcaag
      661 aagaccgtct acaagcccct ggaaacctac gcagtcggcc gcaacgaaat cgtcgctggg
      721 accgtttcgc cgagctatct attgccgatt catggccttc agcgtctcga gaagatcatg
      781 gacgaatatg ttggcggcat tggcgcccac tacacgacta acgaaccgct acttacccgc
      841 ggaatcgagt tgctgatcat gctgaaggaa gatctcgcct atctcggggc cgaagatctt
      901 caccagcttc agcgggcgtg ggaacttcag caccgggtct tggcctcgga aagcgtgacg
      961 cagcacacgt tgtttcgaac agaaacgcgg tggccggggt attactaccg ggcggatcac
     1021 ccgaagctgg acgacaagga ttggcattgc ttcaccctgt cccgctacga tcggtattcc
     1081 gggaagtggg agatggagaa ggctccggtg tatcacatca tcgactgatc gcgcggctgc
     1141 tcagagcgct gcgtctcccg gagcatgctc tgggctacag tttcatgagc gccagccccc
     1201 gccacccagt gcagcctgca aagatcatcc tgtccgagac agtttgattt caggaaagaa
     1261 ccgcaatgtc ccaactcgta ccgccgcaca attcggagca ggtcaagcca ctgctgctgc
     1321 cggagacgga gcgggctgag gaacgcaagc gggctgagaa actcaggaaa attcctctcg
     1381 actcacgttc ggtctccgac gtcttcatgc tggcaatggg ggcctatacg ccgctcgatg
     1441 ggtttatggg gcatgatgat tggcgcggtt cgtgcctgga tatgaaactg accaacggcg
     1501 tcttctggcc gatcccgatt acacttcccg taaacaaaga ccttgccgat agcatccata
     1561 ccgaagagga ggtcacgctc atcgacgcgg agaccggtga aatactcgcc gtcatggacg
     1621 ttcacgagaa gtattcaatc gacaaacaac tagaggccga gcacgtctat cgcaccgccg
     1681 atccgaagca tccaggcgta gcgaaggtac tggatcaggg ggatgtcaac ctggccgggc
     1741 ccctcatggt gctgagcgaa ggcgaatatc ctgccaaata cccggacctc tacatccgcc
     1801 ctgccgagtc gcgcgccatg tttcttgaga gggggtggtc gaatgtcgct gcctttcaaa
     1861 cccgaaatcc catgcaccgt agccatgagc acctggttaa aatcgcggtg gaggtatcag
     1921 acggcgtttt catccatcag gtgcttggga aattgaaagc aggcgacatt ccagccgatg
     1981 ttcgcacccg agcaatccag gcgatgatcg acaactattt ccgcccggga acggtaattc
     2041 aggcaggcta tccgatcgag atgcgctatg cgggcccgcg cgaggcgctg cttcatgccc
     2101 tcattcgcca gaactttggc tgctcgcacc ttatcgtcgg gcgcgaccat gctggcgtcg
     2161 gcgactacta cggtcccttc gatgcacatc acatcttcga tcaactgtgg gacggcgcac
     2221 tttataccaa gccgctgaag atcgacatca ctttttactg tcggaagtgt tatggaatgg
     2281 cgacggcgaa gacctgtccc cacgatccga aacatcacat ttcgatctcc ggcacgcagc
     2341 agcgtgaaat gctctccacc gatgcggaca taccgccaga gttcagccgg ccggaggtgg
     2401 tggcgatcct caaggagtat tacgcaggtc tgcgatcggc aggcttgggt cgg
//
LOCUS       DASSLD010000422         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_491151,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000422 DASSLD010000000
VERSION     DASSLD010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491151"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFK23_07665"
     CDS             complement(<1..293)
                     /locus_tag="VFK23_07665"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET7318993.1"
                     /translation="MKLGYIGLGKMGYNMAELLLEKGHRIVAYNRSAEPVKKIAERGA
                     QTAESIRSLVAALEPPRLAWIMVPYQAVDDVLNELEPLLAKGDTVIDGGNSPY"
     gene            complement(474..926)
                     /gene="rpiB"
                     /locus_tag="VFK23_07670"
     CDS             complement(474..926)
                     /gene="rpiB"
                     /locus_tag="VFK23_07670"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET7318994.1"
                     /translation="MNIAVGADHGGFKLKGVLTEHLKKLGHGVLDLGTHDENPVDYPD
                     YARAVARAVLDKKAGRGILICGSGVGAGVAANKFPGIRAAVCHDTYSAHQGVEHDDMN
                     VLCLGARVVGPELAKEIARTFLEAVFSGAERHVRRLGKVTEIEKEFMK"
     gene            complement(1159..1371)
                     /locus_tag="VFK23_07675"
     CDS             complement(1159..1371)
                     /locus_tag="VFK23_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7318995.1"
                     /translation="MKCSFLTGTHVLTCRANKDINYIPSAFELAEYCLTEKAKMCPYP
                     TLMSFGIVHQPGAEGQQKEKPHLKAA"
     gene            complement(1568..>2121)
                     /locus_tag="VFK23_07680"
     CDS             complement(1568..>2121)
                     /locus_tag="VFK23_07680"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276855.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     alpha"
                     /protein_id="HET7318996.1"
                     /translation="GVFPNYSGSRYDRQGGPRLRNATVTTVAPTGTLSIIAGCSSGIE
                     PLYALSFTRHVLGDVELPEVYQYFLETAKRRGFFSEGLLMRVMAGESIQRMAEVPEDV
                     KRVFVTAFDIAPDWHVRMQAAFQRHTDNAVSKTINFPQHAAKEDVRRAFMLAYEEGVK
                     GITIFRSGSKSEQVLTCADPLYC"
BASE COUNT          428 a          623 c          570 g          500 t
ORIGIN      
        1 ttgtacggtg aattgccgcc gtcgatgacc gtatcgccct tcgccaggag cggctcaagc
       61 tcattcagca cgtcgtcaac cgcctggtag gggaccatga tccaggccag ccgcggaggt
      121 tcgagggctg cgaccagtga gcggatcgat tccgcggtct gcgccccccg ctccgcgatt
      181 ttcttcaccg gctccgcgct cctgttgtat gcgacgatcc ggtgtccctt ctccagcagg
      241 agttcagcca tgttgtagcc catcttgccc aagccgatgt atccgagttt catatttcat
      301 ttccttttct tttgacgtca ttcccgcccc tgcttcaatc atgctggggc tggctccagc
      361 gggaatccat tttaaagctc tgtttgatgg aagccaatgg atacctgctt tcgcaggtat
      421 gagagacaaa taatttccgg tgtttcaaac tcgcgacacg caactcgcaa ccattatttc
      481 atgaattctt tttcaatctc cgttaccttg ccgagccgcc tcacatgccg ttcagcgccg
      541 gagaacactg cttccaggaa tgttcgcgcg atctcctttg ccagttccgg gcccacgacc
      601 cgcgccccga ggcagagcac gttcatgtcg tcatgctcca cgccctgatg ggcggaatag
      661 gtgtcatggc atacggcggc gcggatgccg ggaaatttat tcgccgcgac gccggccccg
      721 acgccgctcc cgcatatgag gattccccgc ccggctttct tgtccaggac ggcccgcgca
      781 acggcgcgcg cgtaatcggg ataatccacc gggttttcat catgcgtgcc cagatcgagc
      841 accccgtgcc cgagcttttt cagatgctcc gtgagcacgc ctttaagctt aaatcctccg
      901 tgatcggccc caaccgctat attcattatc gtcaccagga aagcaaatgc ggtaaactct
      961 tggacaataa aaagcatatc atagccactt catatgtcaa gaagacacgc atacgaaggg
     1021 attacggcag ttgccgcagg aatcacgatg caaaggtcga gatgatgcag gtcttggtca
     1081 agggatgcac agcgcttcgg agtggactat gcgcgcaagg gggaagtgat ttgttttttt
     1141 caaataaaag tgtttctcct aagccgcctt caaatggggc ttctcctttt gctgcccttc
     1201 tgccccgggc tgatgcacga tcccgaacga catcagcgta ggataggggc acattttagc
     1261 tttctcggtc agacagtatt ccgcaagctc gaaggcgctg ggtatataat tgatgtcctt
     1321 gttcgccctg caggtcagga cgtgggtgcc ggtcaaaaaa gagcatttca tagcgcctcc
     1381 tcttccccgg ggccattgtc ggactctgga atatcgtgcg gtacattttt tttcataatg
     1441 ccgcatttca ttcccgaggg acagattaca ttatagcatc atttttttgc tctgccgaag
     1501 gacggaataa atagcggcac cgtgatgcag gacagatcgg gatgagtggg gcttataact
     1561 ctttccccta gcaatacaac ggatcggcgc aggtgagcac ctgctcgctc ttgctgccgc
     1621 tgcggaaaat ggtaatgccc ttcacgccct cttcataggc gagcatgaag gccctgcgta
     1681 catcttcctt cgcggcgtgc tgcgggaaat tgatggtctt ggatacggca ttgtcggtat
     1741 ggcgctggaa cgccgcctgc atgcggacat gccaatcggg agcgatgtcg aaggccgtca
     1801 cgaacacgcg cttgacgtcc tccggcactt cggccatgcg ctgtatggac tcgccggcca
     1861 tcacgcgcat gagcaggcct tcgctaaaaa agccgcgccg ctttgcagtc tcgaggaaat
     1921 actggtagac ctccggcagc tcgacgtcgc ccagcacgtg tcgtgtgaaa gagagcgcgt
     1981 agagcggttc gatcccgctt gagcagcccg cgataatgga cagcgtccct gtcggggcga
     2041 cagttgtcac cgttgcgttc ctcagcctcg gccccccctg ccggtcgtat ctgcttccgg
     2101 aatagttggg gaaaaccccc c
//
LOCUS       DASSLD010000423         4450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1574785, whole genome shotgun sequence.
ACCESSION   DASSLD010000423 DASSLD010000000
VERSION     DASSLD010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574785"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VFK23_07685"
     CDS             complement(<1..548)
                     /locus_tag="VFK23_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HET7318997.1"
                     /translation="MMNDDKLVIAGIEFNSRLWVGTGKYKDFVETKKAIEASGTDVVT
                     VSVRRVNIIEKGKENLLDYIDPKKYKILPNTAGCYTAKDAIRTAMLAAEAGITKLVKL
                     EVIGDQKTLFPDNEATLEAAKVLVKEGLIVLPYTSDDPIMAKKLVDLGCPAVMPLAAP
                     IGSGLGIRNPYNILIIMETVKVP"
     gene            complement(627..827)
                     /gene="thiS"
                     /locus_tag="VFK23_07690"
     CDS             complement(627..827)
                     /gene="thiS"
                     /locus_tag="VFK23_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HET7318998.1"
                     /translation="MNISLNGEKKTVPDNITVMGLLEFLNIQHQRVAVELNMQIVKKD
                     KYSVTSVREGDSLEVVSFMGGG"
     regulatory      complement(910..1012)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1096..1527)
                     /locus_tag="VFK23_07695"
     CDS             complement(1096..1527)
                     /locus_tag="VFK23_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HET7318999.1"
                     /translation="MPYRYLDDVAIADVAFEAEGKTLRELFESSALALTNIMISKVET
                     LEPKVIRCFEVTAENPEMLLYHFLQELVFYKDAERLLFGKFELAVEQEVPAWRLRVAA
                     VGEELSYEKHELLADAKAVSFHNFSVRKTADGWRADVIVDV"
     gene            complement(1654..3138)
                     /locus_tag="VFK23_07700"
     CDS             complement(1654..3138)
                     /locus_tag="VFK23_07700"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016858431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HET7319000.1"
                     /translation="MAMPAEKMTMHKPDFTRITDVTWDLPKAFKAGMRVPARIIATRK
                     LLDAMDLQVYDQISNVATLPGIQKFALCMPDGHSGYGFPIGGVAAFDVNEGGVISPGG
                     IGFDINCGMRLVLTNLTRDEVAPHLKTLVEKLFRRVPAGVGSRGFVDISREEFRNRVA
                     VEGAEWAVRKGYGWQEDLERTELGGRAGWADKSKISQRSVERGYNQIGTLGSGNHYLE
                     IQWVKPENIFHEKLAAKWGIFPGQIVVMFHCGSRGFGHQIASDYLELFLSVMEKKYGI
                     RILDRELACAPFHSPEGQDYFKAMGCAVNMSFANRQVILHRIREVFSDVFSRSPEDLG
                     MHMVFDIAHNRGTLERHEVNGKTRELIVHRKGATGSYYPGREEIPGPYREDGSPVIIG
                     GSMETGSYLLVGGPRAGETFCSTAHGSGRTMSRTKAKHMVNGRELLRGLEERGIYVRT
                     VSYPGVAEEAGFAYKDIDDVVEAARLAGISMPVVKLVPLGNVKG"
     gene            complement(3434..4030)
                     /locus_tag="VFK23_07705"
     CDS             complement(3434..4030)
                     /locus_tag="VFK23_07705"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7319001.1"
                     /translation="MPVRVYLMRHGEVANGSEKRYNGHIDVDITDNGVRQMHRLAGLL
                     AGKELSAIYSSDLIRSVKGARIIADKTGVPPVSLEVLRERSVGAWEGLTADEIKDRFP
                     EEYRLWRADLINYRPPGGECLLDVQNRILPAYKRIVGSHPDKSIALMLHGGVNRIILA
                     DALGMEMTNLFRIDQAFGALNIIDYFEDGMAVVRLLNG"
     gene            complement(4033..>4450)
                     /locus_tag="VFK23_07710"
     CDS             complement(4033..>4450)
                     /locus_tag="VFK23_07710"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HET7319002.1"
                     /translation="VLFLKYLCLNNLFDSDRVSALLTAPVLARWTQTFMVYNARYGRE
                     DGMGQAFVGHLRTSSMAASSAVAAGLSAFVIVRMDPRSVILAVSLFCAVALVTWLGKR
                     YLIRKLGGVTGDAIGAMTELNEVLVFLLFVMFSTGN"
BASE COUNT          900 a         1362 c         1198 g          990 t
ORIGIN      
        1 ggaaccttca ccgtctccat aatgatcaga atattatagg gatttcttat cccgagcccg
       61 gagccgatgg gcgcggcgag cggcatgacc gccgggcaac ccaggtccac aagtttcttc
      121 gccatgatcg ggtcgtcgct cgtgtagggc aggacgatga gcccctcctt caccagcacc
      181 tttgctgcct ccagggtcgc ctcgttgtcc ggaaacaagg ttttctgatc tccgattacc
      241 tcaagcttga cgagcttcgt gatcccggcc tccgcagcca gcatggccgt acggatggcg
      301 tcctttgccg tatagcagcc cgcggtgttc ggaaggatct tgtacttttt cgggtcgata
      361 taatcgagca ggttctcctt gcccttctcg atgatgttca cccgcctgac cgacacggtc
      421 accacgtcgg tgcccgacgc ctcgatggcc tttttcgtct ccacgaagtc cttgtacttt
      481 cccgtcccca cccagaggcg ggagttaaat tcgatgcccg cgattaccag cttatcatcg
      541 ttcatcatat gctccatagt cctgatattc acgcagactt aagcctgcgg ttacaactca
      601 ttccgcattc cgaattccgc attgtttcac ccgcccccca taaagctcac gacttccagg
      661 ctgtcccctt ccctgaccga ggtcacgctg tacttgtcct tcttcacgat ctgcatgttc
      721 agttcgacag ccacgcgctg gtgctggatg ttcaggaact ccagcagacc catgaccgta
      781 atattgtcgg ggacggtctt cttctcgccg tttagggaaa tgttcataat ctgtggcacg
      841 ctgaagctgt cggcccgccc ccccaaaaaa aaaccacgcc ggaggcgtgg caggaaaggt
      901 taatgatgat ggagcagatg ttcgccttcc ctacgccggc actacccgga tcaggttcca
      961 agggtcgtcc tgtcaaggac tctcagccgt ttcggctccc ccagctacac gatgggtata
     1021 caatacggta tgaaatgatc aaagtcaatg gatttttgcc ccgagggaat tatacaaggc
     1081 ctgaagggag ggcggtcaca catccactat cacgtcggcc cgccagccgt cagcagtctt
     1141 ccgcacgctg aaattgtgga acgacaccgc cttggcatcg gcgagcagtt catgtttttc
     1201 gtacgataat tcttctccta cggccgccac tcgaagacgc catgccggga cctcctgttc
     1261 gacagcaagt tcgaacttgc cgaagagcag ccgctccgcg tccttgtaaa agacgagttc
     1321 ctggagaaag tgatagagca gcatttcggg attttcggcc gtcacttcaa agcacctgat
     1381 cacctttggc tcaagggtct ccactttgct gatcatgatg ttcgtcaacg ccagggccga
     1441 agactcgaaa agttcccgca gcgttttccc ctccgcctcg aaggccacat cggctatcgc
     1501 aacgtcgtcc agataccggt acggcatatc acctctctaa aaaaaccaac cacggagtca
     1561 cggagaaaag tccgtgtaaa ggatttacct tctcccctcg gcaattctcc tccgtgcctc
     1621 cgtgtctctg tggtaaattt gccttgaccc tattcaacct ttgacattcc cgagcgggac
     1681 cagtttcacg acgggcatcg aaattcccgc gagcctcgcg gcctcgacca cgtcatcgat
     1741 gtctttgtac gcaaacccgg cctcttccgc cactcccggg tagctcacgg tccgcacata
     1801 aataccccgc tcctcgaggc cccggagcag ttccctgccg ttcaccatgt gtttcgcctt
     1861 ggtcctgctc atggtcctgc cgctgccgtg ggccgtcgaa cagaaggtct ctcctgcgcg
     1921 gggtccgccc acgagcagat agctgcccgt ttccatggaa ccgccgatga tgaccggcga
     1981 tccgtcttcc cggtacggcc ccgggatctc ttcccgtccc ggataataac ttcccgtcgc
     2041 ccctttccga tggacaatca gctctctggt ctttccgttc acctcgtgcc tttccagggt
     2101 ccctctgtta tgagcaatat cgaagaccat gtgcatgccg aggtcttccg ggctccggct
     2161 gaagacgtcc gagaacacct cgcgtatccg gtggaggatc acctgccggt tcgcgaaact
     2221 catgttcacg gcgcagccca ttgccttgaa gtagtcctgc ccttccggcg aatggaacgg
     2281 ggcgcaggcg agttcgcggt cgaggatcct gatgccgtac ttcttctcca tgactgaaag
     2341 aaaaagctcg agataatcgc tggcgatctg gtggccgaaa ccgcgggacc cgcagtggaa
     2401 catgaccacg atctggccgg gaaagatgcc ccattttgcg gccagctttt catgaaagat
     2461 gttctccggc ttgacccact ggatctccag atagtggttt cctgagccga gtgtcccgat
     2521 ctgattgtaa ccgcgttcga cggacctctg ggatatcttg gatttatctg cccagccggc
     2581 cctgccgccc agttcggtac gttccaggtc ctcctgccat ccataacctt tcctgactgc
     2641 ccactccgcg ccctcgaccg caacgcggtt gcggaactcc tcacgggaaa tgtccacgaa
     2701 gcccctgctc cctacccccg cgggcacgcg ccggaaaagc ttctcaacga gcgttttcag
     2761 atggggggca acttcgtcac gggtcaggtt cgtcagcacg agccgcattc cgcaattgat
     2821 gtcgaagccg atgccgcccg gcgaaatgac gccgccctcg tttacatcga aggcggcaac
     2881 gcccccgatc gggaatccgt atccgctgtg gccatcgggc atgcataagg cgaatttctg
     2941 gatccctggc agcgtggcca cgttcgatat ctggtcatag acctgcaggt ccatggcatc
     3001 gagcagtttc cgcgtggcga tgatccgggc gggcacgcgc atgcccgctt tgaaggcctt
     3061 cgggaggtcc cacgtgacat cagttatcct ggtgaagtcc ggtttgtgca tcgtcatttt
     3121 ttccgcaggc attgccatac cttgcctccg ggaagaaaca tcatgtatgt ctacttaata
     3181 caatcacttt tttatcgagt cgtcaaggat gggggggatg agaagagatg cgatatgata
     3241 cttttgcctg agtagactgg gaaaaatatt ttaaggtgtc atcctgaacg aagtgaagga
     3301 tctgagctgt atggagaacc gagattcttc gctgcgctca ggatgacgat tcccggggtt
     3361 ttccgacttt tcaatagcta ttagtattca ggagagatgc tgaccggcac agcagagact
     3421 acggcagatt ttgtcagccg ttcaacagcc tgaccacggc catgccgtct tcgaaatagt
     3481 cgatgatgtt cagggccccg aaggcctggt cgatccggaa aaggttcgtc atctccatgc
     3541 cgagggcgtc ggcaaggatg atgcggttca cgccgccgtg gagcatcagg gcgatcgact
     3601 tatcgggatg agagccgacg atccgtttgt acgcggggag gatgcggttt tgaacgtcaa
     3661 gcaggcattc gccgcccggc ggacggtagt tgatcaggtc ggcgcgccac agccggtact
     3721 cctcgggaaa gcgatctttg atctcatcgg cggtcaaccc ctcccaggcg ccgacgctcc
     3781 gctcccggag cacctcgagg gaaaccggcg gcactcctgt tttgtcggcg atgatgcgcg
     3841 cgccttttac ggaccggata agatcgctgg aatatattgc cgacagttcc tttccggcaa
     3901 gcagaccggc aaggcggtgc atctgacgca cgccgttatc ggtgatgtcg acgtcgatgt
     3961 ggccgttgta acgcttttcg gaaccgttcg ccacttcgcc gtggcgcatg aggtagaccc
     4021 tgacaggcat ggttaattcc cggtggagaa catcacgaag aggaggaaca ccagcacctc
     4081 gttcagctcg gtcatcgcac cgatcgcgtc cccggtcacc cctccgagtt tcctgatgag
     4141 gtaccgtttc ccgagccagg tcacgagcgc aacagcgcag aaaaggctga cggcgaggat
     4201 gaccgaccgg ggatccatgc gcacgatcac gaatgccgac agccccgcgg ctaccgccga
     4261 tgaagcagcc atgctgctcg tacgcagatg gccgacgaaa gcctgcccca tgccgtcttc
     4321 ccggccgtac cgggcgttgt acaccatgaa cgtctgcgtc cagcgggcca gcaccggcgc
     4381 cgttaaaagc gcggaaaccc ggtcgctgtc aaagagattg ttgaggcaga ggtacttgag
     4441 gaacaggaca
//
LOCUS       DASSLD010000424         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1624687, whole genome shotgun sequence.
ACCESSION   DASSLD010000424 DASSLD010000000
VERSION     DASSLD010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624687"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="VFK23_07715"
     CDS             <1..908
                     /locus_tag="VFK23_07715"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HET7319003.1"
                     /translation="SLRYEQSDILKRGIFNFQEPWFLNRRLDAKFSLVWSDAKRLSAD
                     LFNRELLYKTRKTSASFGIEKSYGKLKPSLTYQFENVVNYNVLAAAQITPDDSGRVLV
                     SSLSPALIWDLRDDVFNPRRGALYGIILKEALREFFSEADFTKLTMQASWFLPIDSSV
                     AAFSARAGMAWPFRGTPAVPLHERFYVGGNTTVRGYTQDSIGPTALDPSGALIPQGGA
                     SMAVFNFELRLNPGEGLGFVLFTDAGNAWSGQEMNIRDLRSSYGAGIRYGTPVGPLRI
                     DYGQKISRRSGESPGELHFNIGNTF"
     gene            1093..>2652
                     /locus_tag="VFK23_07720"
     CDS             1093..>2652
                     /locus_tag="VFK23_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7319004.1"
                     /translation="MPLHKKIIFIVGAGMLVIMTAVVLAELNVLSAFSSITGEVENTS
                     RELRQIDDIAAAVAGMDANLHDAVSTRDVRYRQDYEIERTTVHRLLDDFQRINVDADD
                     RRVIITLRSEITALESVADRLLALKDPLHKDGHMTVSLLKEADHITEKINRDIDNHFR
                     DEQALQIARLPDFTSLQQKKVLLFSSSLLVLTVGFLSVFGVYLHRKIAAPLSALWKGA
                     SEITRGNLDYQMQLSGKSDVAQLAEQFNDMAQKLKESYTDLEKKLLERTNELAAIDAV
                     ALTLSKAGNLRDMLSRSLDKIMENMAGLEPRGGVFLCEPDGETLRLVAHKGLSPQFVE
                     REGTIRMGECLCGMVAQSGEILYSEKGCVDLRHSRGHDAGSHGHIIIPVKSRGIVFGV
                     VFLYPQKEFSPKPSDLQMLDTIGTQLGMAVENLRFYAEVKESSEKFWDLFENSRDILF
                     TMDSSGVLTAVNKEAEKFSGYSKTELVGKNVLDFLTSDGAETARRMLADEGGAASQTI
                     EFEVVKRDGGRA"
BASE COUNT          544 a          823 c          786 g          499 t
ORIGIN      
        1 cgagcctgcg gtacgaacaa agcgacattc tcaagcgcgg gatattcaat ttccaggagc
       61 cctggttcct gaaccgcagg ctcgatgcga agttcagcct cgtgtggtcc gacgcaaaac
      121 gtctgagcgc cgatttgttc aaccgggagc tcttatacaa gacgcgaaag acctccgcct
      181 ccttcggcat cgagaaatcc tatggaaaac tgaagccgtc gctcacctac caattcgaga
      241 acgtcgtcaa ttacaacgtg ctggccgcgg cccagatcac ccccgacgac agcggccgcg
      301 tgctggtgag cagcctgagc cccgccctga tatgggacct ccgcgacgac gttttcaacc
      361 cgcgaagggg ggccctctac ggaatcatcc tcaaggaggc gctccgggag tttttctcag
      421 aagccgattt caccaagctg acgatgcagg ccagctggtt cctgcccatt gactcctccg
      481 tagccgcctt ttccgcaagg gccgggatgg catggccgtt ccggggtacg cccgcggtcc
      541 cgctccatga gcgtttctac gtcggcggca acacgacggt gcgcgggtat acccaggact
      601 ccatcggccc aaccgctctg gacccgtccg gcgccctgat cccccagggc ggcgcgagca
      661 tggcggtctt caacttcgag ctccggctga accccggcga aggcctcggg ttcgtgctgt
      721 tcaccgatgc cggcaacgcc tggtcaggtc aggagatgaa tatccgggac cttcgttcct
      781 cttacggcgc cggcatccgg tacggcacgc ctgtcggtcc cttacggatc gattacggcc
      841 agaaaatcag ccgccgatcc ggcgaaagcc cgggagagct tcatttcaac atcggcaata
      901 cgttctaaat ggagagcagg gtccgatatc cggggtccag ggcccaggac atggttttac
      961 tccgaactcc gaactgtgaa ctccgaactt tgctccctgt actcccggcg ttgatcgtgg
     1021 tattattctg tttggagcat tggcaccgcg aaagagcgcg gttccgcgcg tgacagaaag
     1081 gtcctgcagc acatgccttt gcacaaaaaa atcattttta tcgtcggggc cggcatgcta
     1141 gtgatcatga ccgccgtcgt actggcggaa ttgaacgtcc tgtcggcgtt ttcctccatc
     1201 acgggtgaag tcgagaacac ctcgcgggag ctgcggcaga tcgatgacat cgccgcggcg
     1261 gtagccggca tggacgccaa cctccatgac gcagtctcta cgcgggacgt gcgctaccgg
     1321 caggattatg aaattgaacg gacgacggtc cacaggctcc tggacgactt tcagcggatc
     1381 aatgtcgatg ccgatgaccg gcgcgtgatt attaccctgc gctccgagat caccgcgctc
     1441 gagtccgtgg ccgatcgcct gctcgcgctc aaagacccgc ttcataagga cgggcacatg
     1501 accgtgagcc tcctcaaaga ggccgaccat atcacggaga agatcaaccg tgacatcgac
     1561 aatcacttcc gggacgagca ggctctccag attgcccgac tccccgactt cacctcgctc
     1621 cagcagaaaa aggtcctcct cttctcctcc tcgctgctcg tcctgaccgt cggttttctc
     1681 tcggtcttcg gagtctatct tcatcggaag atcgcagccc ccctgagtgc attatggaag
     1741 ggcgcatcgg aaatcacccg gggaaacctc gactaccaga tgcagctgtc ggggaagagc
     1801 gatgtggccc agcttgcgga gcagttcaac gatatggccc agaagctgaa ggaatcctac
     1861 accgacctgg agaagaagct gctcgagcgg accaacgaac tggctgcgat cgacgccgtc
     1921 gcgctgaccc tgagcaaggc gggaaatctc cgggacatgc tgtcgcgctc cctggacaag
     1981 atcatggaaa acatggcggg cctggaaccg cgggggggcg tgttcctctg cgagcccgac
     2041 ggcgagacgc tgcgcctcgt ggcgcacaag ggcctttcgc cgcagttcgt cgagcgggag
     2101 ggaacgatca ggatgggaga gtgcctctgc ggcatggtgg cccagagcgg ggagatcctc
     2161 tactccgaaa aggggtgcgt cgacctgcgg cattcgcgcg gccacgacgc cggttcccat
     2221 gggcacatca tcatccccgt caagtcgcgc ggcatcgtct tcggcgtcgt ctttctctat
     2281 ccccagaaag agttctcccc caagccctct gacctccaga tgctggatac aatcggaacc
     2341 cagctcggta tggccgtcga gaacctgcgg ttctacgccg aggtgaagga gtcgagcgag
     2401 aagttctggg accttttcga gaattcccgg gacatcctct tcaccatgga cagctcgggc
     2461 gtcctgacgg cggtgaacaa ggaggcggaa aaattctcgg gctacagcaa gaccgaactc
     2521 gtcggaaaga acgtgctcga ttttctgacg tccgacggtg ccgaaacggc gcgacggatg
     2581 ctggccgacg agggtggagc cgcaagccag acgatcgagt tcgaggtggt caagcgcgac
     2641 ggaggtcgcg cc
//
LOCUS       DASSLD010000425         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1095986, whole genome shotgun sequence.
ACCESSION   DASSLD010000425 DASSLD010000000
VERSION     DASSLD010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095986"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VFK23_07725"
     CDS             complement(<1..1016)
                     /locus_tag="VFK23_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008382865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7319005.1"
                     /translation="MGKKKKTIRSIPQQRTPVREQDARVRVKNFDEVNCGYSLEEALN
                     EAERCLLCPDPDCVAGCPVNINIPKFIEAITAKDFRGAYNVVTASNLLAAVCGRVCPQ
                     ETQCEGVCVVGESLEPVAIGRPERWVGDQAIKEKWANVPYVEPSGFKVGIVGSGPAGM
                     ACAADLAKAGCEVTVYEAFHQAGGVLKYGIPDFRLPNAVVDAEIENLRKLGVKFECNT
                     LVGRLFTIEQMRDELGFDAVFIGTGAGYPSMLGIPGDTLNGVLSANELLTRCNLMRAK
                     EFPNYDTPLPLGKHVAVIGAGNTAMDAMRVSLRLGAEKVYCIYRRSRAECPARAEEVH
                     HAEE"
     gene            complement(1093..2037)
                     /locus_tag="VFK23_07730"
     CDS             complement(1093..2037)
                     /locus_tag="VFK23_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008382863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HET7319006.1"
                     /translation="MADANPGPGFKIMVREDFSDVTYSLVIHHPLMAKAARPGQFVIV
                     MSHESGERVPLTIADFDREKGTVTLVIQAVGKSTKEMQQICQVGTSLYGVGGPMGVPS
                     PLTGKKKVVCVGGGLGVAPIFPQARGFKENGAYVIGILGFRTKNLIFWEDKFRACCDE
                     LILCTDDGSAGMKGFVSDGIKLAIEGHGNIDEVVAIGPPIMMKACAETTRPHAIKTIV
                     SLNPIMVDGTGMCGGCRVKIGNEIKFACVDGPDFDGHKVDFNDLMMRLKRYAEEEKVA
                     LDRWSENCRMTQRTRPVLKGAEAADLTLPDPFEDALGG"
     gene            2238..>2407
                     /locus_tag="VFK23_07735"
     CDS             2238..>2407
                     /locus_tag="VFK23_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET7319007.1"
                     /translation="MSRIIEILLDEHRNIEKLLHIVEQELEIFDRGEPPDYEILQAII
                     DYFQDYPESYHHP"
BASE COUNT          520 a          728 c          630 g          529 t
ORIGIN      
        1 tcttcctcgg cgtgatgaac ctcttccgcg cgtgcagggc actccgcccg agaccgtcga
       61 tagatgcagt acactttctc ggcgccgagg cgcaggctga cgcgcatcgc gtccatggcc
      121 gtgttgccag cgccaatcac ggcgacgtgc ttcccaagtg gaagcggggt gtcgtaattt
      181 gggaattcct tcgcccgcat caagttgcag cgggtcaaca gctcgttagc ggaaagcacg
      241 ccattaaggg tgtcgccagg gatgccgagc attgagggat agccggcgcc ggtgccgatg
      301 aagacggcgt cgaaaccgag ttcgtcgcgc atctgttcga tggtgaacag ccggccgact
      361 agggtattgc actcgaactt gacgccgagc ttgcgcagat tttcgatttc cgcatcaacc
      421 accgcgttgg gcagacggaa atcggggatc ccgtacttga gaacgccgcc ggcttggtgg
      481 aaggcctcgt aaacagtcac ctcacatcca gctttagcca agtccgccgc acacgccatg
      541 cccgcgggac ccgaaccgac aatcccgacc ttgaagccgc tcggttcgac atagggaacg
      601 ttggcccatt tttcctttat ggcttggtcg ccgacccagc gttcggggcg gccgatcgcc
      661 accggctcga gtgactcgcc gaccacacag acgccctcac attgcgtctc ctgcgggcag
      721 acccggccac acactgccgc caagagattg cttgcggtca cgacgttata ggcaccacgg
      781 aaatccttgg cggttattgc ctcgataaat ttcgggatgt tgatattgac ggggcagccg
      841 gctacgcagt ccgggtccgg gcataagagg catcgctccg cctcgttgag cgcctcttcc
      901 agggaatagc cgcaattcac ctcgtcgaaa ttcttcaccc gcacccgcgc atcctgttcg
      961 cgcacaggag tccgctgctg gggaatgctt cggattgttt ttttcttctt gcccattcta
     1021 tcagtcctgt gcagttaaca agtcatccca agcgttgagc aaccgatacc gaaaccttga
     1081 cggaggctca cgctatcccc ccaacgcgtc ttcaaaggga tcgggcaatg tcaaatcagc
     1141 tgcttcagcg ccctttaata ccggtcgggt ccgctgcgtc atccgacagt tttccgacca
     1201 ccgatccagc gcgacctttt cttcttcggc gtagcgctta aggcgcatca tcagatcgtt
     1261 gaagtcgacc ttgtgcccat cgaaatcggg accatcgacg catgcaaact ttatttcatt
     1321 gccgatcttg acccggcagc caccgcacat cccggttcca tcgaccatga tcgggttaag
     1381 gctgacgatc gtcttgatcg catgcgggcg cgtggtttcg gcgcaggcct tcatcatgat
     1441 aggcgggccg atggccacca cttcgtcgat gttgccgtgc ccctcgatgg caagtttgat
     1501 gccgtcgctg acaaagccct tcattcccgc ggacccgtca tccgtgcaca ggattagttc
     1561 atcacagcag gccctgaact tgtcctccca gaaaatcagg ttcttggtac gaaagccgag
     1621 aatgccgatc acataggcgc cattctcttt aaatccacgg gcctgcggga agattggtgc
     1681 aacccccaac ccgccgccga cacagaccac ctttttcttt cctgtgagcg ggcttggcac
     1741 ccccatgggc ccacccactc catagagaga agtccctacc tggcagatct gctgcatctc
     1801 cttggtcgac ttcccaaccg cctgtatgac cagggtgacg gtaccctttt cgcggtcgaa
     1861 gtccgcgatg gtcaacggga cccgttcgcc ggactcatgc gacatcacaa tgacgaattg
     1921 acctggccgc gcggctttcg ccatcaacgg gtgatggatg accaaggaat aggtcacgtc
     1981 ggaaaagtct tcacgcacca taattttgaa gcctggccct ggattggcgt cggccatcga
     2041 ttcgactccc gagcgaagag gaacattttc cgccgaaaaa atatcgaata gttgacccaa
     2101 tcacgctttg acataacgca aagccaggcg tcggattgac gttaatctta tataattttc
     2161 aaaagtatga gggcccccga atagagatta tcgctgccag atcaaaatgt tattcggacg
     2221 gcacctggtc tagatcaatg tcaagaatta tcgagattct actcgacgag caccggaaca
     2281 ttgaaaagct gcttcacatc gtggagcagg aattggaaat ttttgatcgc ggcgagccac
     2341 cagattatga aattttgcag gccatcatcg attatttcca agattacccc gagagctacc
     2401 atcatcc
//
LOCUS       DASSLD010000426         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_980716,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000426 DASSLD010000000
VERSION     DASSLD010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980716"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFK23_07740"
     CDS             complement(<1..210)
                     /locus_tag="VFK23_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319008.1"
                     /translation="MRTSSRSDLPERHGRTALLWDESFLWGVMARKALHARGLAFDMI
                     RAKDVRDGRLHDYSLLFVPGGWASNK"
     gene            complement(188..>2094)
                     /locus_tag="VFK23_07745"
     CDS             complement(188..>2094)
                     /locus_tag="VFK23_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HET7319009.1"
                     /translation="SSESEVKKTAIIYITDSGERLAERLQGFLPDAGLVKFGMGTVPE
                     LWSESSSLVFIMAAGIVVRTIAPLLKDKKSDPAVVVLDEQGKFAVSLVSGHVGGANEL
                     AKEIAGFLKGESVVTTASDVNGLPSIDLWARDNGLLIENWDVLPQVSTRYVNNGGLRI
                     YSDITLELPAEFLRVSDPRFADAIVSNRRDVYAGSPRCTITGEGCATDACRVKGQVYL
                     RPVNLVVGIGCNSGTTPDEIEAAVLSTLEEHNLVSLSVHSIATIDIKRDEPGLKAFVQ
                     KNDFSLRTFNHQELNRVEGIEKSDVVMRATGAIGVAEPAALLAAGAERLLVPKQKKGN
                     VTVAIAEMKTMRNAECGMRKEQEKPHTGAIYVVGTGPGSLDHITPAAREAIGKSDVIV
                     GYGTYLDLITELLQGKEVLSTGMTQEVDRCRKAVELAKSGKAVSIISGGDPGVYAMAG
                     LVFEILKSQESEVRSQEVKGGRQGLDEQNNSTFRIPHSALKVEVIPGISALNASASRL
                     GAPLMHDFACISLSDRLTPWEVIEKRVEAAAAADFVIVLYNPRSKGRAGHFNRAREII
                     LKHRKPSTPAGLVKGAMREQETVIVTDLANMQDSDIDMQTTVIIGNSKTFAWNNLMVT
                     PRGYENKFTI"
BASE COUNT          402 a          656 c          561 g          475 t
ORIGIN      
        1 tttgttcgac gcccagccgc cgggaacgaa cagaagggaa taatcgtgca gccggccgtc
       61 cctgacatcc ttcgcgcgga tcatatcaaa ggccagtccc cgggcatgga gggccttgcg
      121 cgccatgacg ccccacagga aagactcgtc ccagaggagc gcggtcctgc cgtgccgttc
      181 aggaagatca gatcgtgaac ttgttctcat accctctcgg tgttaccatc agattgttcc
      241 atgcgaatgt ttttgagttg ccgatgatca ccgttgtctg catgtcgatg tcgctgtcct
      301 gcatattcgc gagatcggta acgatgaccg tttcctgctc gcgcatcgcc cctttgacga
      361 ggcccgccgg cgtcgagggc ttccggtgct tcaggatgat ctctcttgcc ctgttgaaat
      421 gcccggcccg ccccttgctc cgcgggttgt acaggacgat gacgaagtcc gcggctgcag
      481 cggcttcgac cctcttttcg atcacttccc agggcgtcag ccggtcggac aggctgatgc
      541 aggcgaaatc atgcatgagc ggagcaccga gccgtgatgc gctggcattg agggccgaga
      601 tgccgggaat gacctcaacc ttcaatgcgg aatgcggaat gcggaatgtg gaattgttct
      661 gttcgtccag tccttgcctt ccgcctttta cctcctgact cctgacttcc gactcctgac
      721 ttttcagtat ctcgaacacc agtccggcca tggcatagac gcccggatcg ccgccactga
      781 tgatggagac tgccttgccg ctttttgcca gttccaccgc tttccggcag cggtccacct
      841 cctgcgtcat ccccgtcgaa aggacctcct tgccctgcag gagttccgta atcaaatcga
      901 ggtaggttcc gtagcccacg atgacgtccg actttccgat tgcctcgcgg gcggccggcg
      961 ttatgtgatc aaggctcccc ggcccggtgc cgacgacata aattgcaccg gtgtgaggtt
     1021 tttcctgttc tttccgcatt ccgcattccg cattccgcat tgtcttcatt tcagctatcg
     1081 ccacggtcac atttcctttc ttttgtttcg gtaccagcag tctttccgcg cccgcggcga
     1141 ggagcgccgc cggctccgca acaccgatcg cgcccgtcgc cctcatcacg acatccgatt
     1201 tctcaatccc ctcaacgcgg ttcaactcct ggtgattgaa cgtacgcagg gaaaaatcat
     1261 ttttttgaac aaaagccttc agacccggct cgtcgcgctt gatatcaata gtggcgatgg
     1321 aatgaacgga caaagaaacc aggttgtgtt cttcgagcgt cgaaagaacc gccgcctcga
     1381 tctcgtccgg ggtcgttccg ctgttgcagc caatcccgac aacgagattg acgggtctaa
     1441 gatagacctg tcccttcacg cggcaggcgt ccgtcgcgca tccttccccg gtaatagtgc
     1501 atctcggaga acctgcgtaa acatcgcggc gattgctgac gatggcgtcg gcgaaccgcg
     1561 gatcggacac ccggaggaac tcggcgggca gttccagagt gatatcgctg tagatccgca
     1621 ggcctccgtt gttgacatag cgtgtactga cctgcgggag cacatcccag ttctcgatga
     1681 ggagaccgtt gtcccgggcc cagaggtcaa tggaaggcag accgttcacg tccgaggctg
     1741 tcgtaacgac ggactcgccc ttcaaaaaac ctgcgatctc cttcgcaagt tcgttggcgc
     1801 ctcccacatg cccgctcacc aggctgaccg cgaactttcc ctgctcatcc agaacgacga
     1861 ccgcagggtc ggatttcttg tccttcagga gaggtgcgat cgtgcggaca acgatcccgg
     1921 ccgccatgat aaaaacaaga gacgaggact cgctccagag ttccgggacg gtccccatac
     1981 cgaatttgac aaggcctgca tcaggaagaa acccttgcag ccgctccgcc agtcgttcgc
     2041 cgctgtcggt gatatatatt atcgccgttt ttttcacttc tgactccgaa ctcc
//
LOCUS       DASSLD010000427         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_7709,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000427 DASSLD010000000
VERSION     DASSLD010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7709"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFK23_07750"
     CDS             complement(<1..380)
                     /locus_tag="VFK23_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HET7319010.1"
                     /translation="MNDFLYLIPALPFAGFIVLALLGRRLRKATVALVGAGSVGVSSL
                     LAVLAGVGFVTEPPEGNAISQTLWTWMQFDGYAPAVSFILDPLSLVMVLVITVVGFLI
                     HLYSAEFMLEEEGYSRFFAYMNLFV"
     gene            complement(413..724)
                     /gene="nuoK"
                     /locus_tag="VFK23_07755"
     CDS             complement(413..724)
                     /gene="nuoK"
                     /locus_tag="VFK23_07755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011330564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HET7319011.1"
                     /translation="MVANVPMEHGLMFAAVLFALGLVGVCARRNIVFMLMSVEVMLNA
                     VGLAFVVAGARWGQVDGQVMFIFILTMAAAEVSVGLALVLQMYHSFKTLDADAVSRMK
                     G"
     gene            complement(824..1318)
                     /gene="nuoJ"
                     /locus_tag="VFK23_07760"
     CDS             complement(824..1318)
                     /gene="nuoJ"
                     /locus_tag="VFK23_07760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HET7319012.1"
                     /translation="MNLLFYISALVGVISTFLVITRKSTVHALLYLIVSLLAVALVFF
                     ALGAPFVAALEVIIYAGAIMVLFVFVIMMLNLGAQAARQEAEWLKPGIWTGPAVLCTV
                     LAGELVYLFTTGRESLSGVAVVTPKQVGIALFGPYVIGVELASVLLLAGLIGAYHLGR
                     PHKH"
     gene            complement(1464..1979)
                     /gene="nuoI"
                     /locus_tag="VFK23_07765"
     CDS             complement(1464..1979)
                     /gene="nuoI"
                     /locus_tag="VFK23_07765"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HET7319013.1"
                     /translation="MFSLLKSIWTVFVTMFRKRVTILYPEEKPYIPPRWRGRIILSRD
                     PDGEERCVACYLCAVACPVDCISLQAAEDAHGRRYPEFFRINFSRCIFCGYCEEACPT
                     YAIQLTTDFEMGEYKRQNLVYEKEDLLISGPGKYHDYNFYRVAGLSIKGKDKGDAENE
                     EKPVDVKGLMP"
BASE COUNT          501 a          609 c          618 g          406 t
ORIGIN      
        1 acaaacaggt tcatgtaggc gaagaagcgg ctgtagcctt cctcctcgag catgaactcg
       61 gctgaataga ggtggatcag gaatccgacg acggtgatga cgaggaccat gacgagcgac
      121 agcggatcga ggatgaatga gaccgcgggc gcatacccgt cgaactgcat ccaggtccag
      181 agcgtctgcg aaatggcgtt tccctcaggc ggctcggtta cgaagccgac tccggcgagc
      241 acggcgagga gtgaggatac ccccacggaa ccggcgccga cgagggcgac cgtggccttg
      301 cgcagacgac ggcccagaag cgccaggacg atgaatccgg caaagggcag ggcgggtatg
      361 agataaagaa aatcgttcat ggttcggagt tcagagttcg gggtaaaaac gactatcctt
      421 tcattctgct taccgcgtcg gcgtccaggg tcttgaagct gtgatacatc tgaagcacga
      481 gcgcgaggcc cacggagacc tcggccgcgg ccatggtcag gatgaaaatg aacatgacct
      541 ggccgtcgac ctgcccccag cgtgcaccgg cgacgacaaa ggcaaggcct acggcgttca
      601 acatcacctc gacgctcatg agcatgaaga caatgttcct gcgcgcacac acgccgacga
      661 gcccgagcgc gaacaggacc gcggcgaaca tgagaccgtg ttccatgggt acgtttgcga
      721 ccatagtact cctcgatgga cgatggacga acgttcgcat tgcgaactag gacgatagac
      781 gaaaaggttt tgcgtccatc gtctctcgtc cctcgtcgcc tcttcagtgt ttatgcggcc
      841 tgcctagatg atatgcgccg atgaggcccg ccaaaagcaa taccgacgcc agttcgacgc
      901 cgatcacgta gggcccgaag agggcgattc ccacctgttt cggcgtgacg acggcgacgc
      961 cggagaggga ctcccggccg gtcgtgaaca gatagacaag ttccccggcg aggaccgtgc
     1021 agaggaccgc cggcccggtc catatccccg gcttgagcca ctcggcttcc tgcctggcag
     1081 cctgggcgcc caagttcagc atcatgatca cgaacacgaa gagcaccatg atggcccccg
     1141 cgtagatgat cacctcaagc gccgcgacga agggcgctcc cagggcaaag aagacgagtg
     1201 ccacggcgag gagcgagacg atcaggtaca ggagcgcatg gaccgtactt ttcctggtga
     1261 tcaccaggaa ggtcgagatg acgccgacaa gtgccgatat gtaaaaaaga agattcatat
     1321 aaaaaccttc aactgcttca acgacgtgtt tgtcattccc gaatgcctct atcgggaatc
     1381 cagtttttca tggtaatgca taattagggc gccggattcc ggcttagatc atgctggaac
     1441 gacggctatt ttgaaatcat cacttacggc atcaaacctt tcacgtccac gggcttctcc
     1501 tcgttctccg catcgccctt gtcctttccc ttgatcgaaa gacccgcgac ccggtaaaaa
     1561 ttgtagtcat ggtatttccc cggtccgctg atgaggaggt cttccttttc atagacgagg
     1621 ttctgtcttt tgtactcgcc catctcgaaa tccgtcgtga gctggatagc gtaggtcgga
     1681 cacgcctcct cgcagtagcc gcagaagatg cagcgcgaga agttgatccg gaagaattcg
     1741 ggatagcgcc tcccgtgcgc gtcctccgcc gcctggagcg agatgcagtc caccgggcac
     1801 gccacggcgc agaggtagca cgctacgcag cgttcctcgc cgtccgggtc ccgggagagg
     1861 atgatcctgc cgcgccaccg gggagggatg taaggtttct cctcggggta caggatcgtg
     1921 acccgcttgc ggaacatggt cacgaagacc gtccagattg attttaagag actgaacata
     1981 agtttttgcc acgaaggcgc ggagacacag agttaaacca tgagccgttg gtaaaaaccg
     2041 gaaattggta caaccacaga aaaaatacta tacgaggtat agatccaacc tcctctgaat
     2101 tctccgtgcc tctgcgtctc tgtggctatc cctt
//
LOCUS       DASSLD010000428         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1188599, whole genome shotgun sequence.
ACCESSION   DASSLD010000428 DASSLD010000000
VERSION     DASSLD010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188599"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VFK23_07770"
     CDS             <1..344
                     /locus_tag="VFK23_07770"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4337 family protein"
                     /protein_id="HET7319014.1"
                     /translation="LAERVATMKQDARMAYERMRDTAASDEARYAKEKEQILEQAKAL
                     EKERDVSRAKDPYFDFGEVLLQLAIVTASIAILSGSSPVYLFSIVMATLGVALSLNGF
                     LLLVTLPFLTP"
     gene            566..1393
                     /locus_tag="VFK23_07775"
     CDS             566..1393
                     /locus_tag="VFK23_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HET7319015.1"
                     /translation="MKTEDIRKYVRDRYAGIAKQGGSCCGPSACCGAPEAAEEASGRI
                     GYTEEEMKSAPRESNLGLGCGNPTALASLREGETVLDLGSGAGFDCFLAAKQVGPGGK
                     VIGVDMTPEMIQKARENARKGNHENVEFRLGEIEHLPAANDSVDIVISNCVINLSPAK
                     DKVFAEACRVLKPGGRAMISDIVLSGDLPEAVAGSLAAYAGCIAGAIRKEEYLRLMEA
                     AGFRDVHVVQESRFEIAETEIDAYARSIAGNFNVPLEAVREAARSVLSVQVFGRKPA"
     gene            1406..2005
                     /locus_tag="VFK23_07780"
     CDS             1406..2005
                     /locus_tag="VFK23_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7319016.1"
                     /translation="MDFKRSFQEIYEKFHPGILRFLARLVGEEEAEDVAQEVFLRVDR
                     GLEGFRGESSMGTWIYRIARNAALDRLRSRPAWLESVHQLQTHDSDENPSDVMSQIPD
                     ERASLERYLIGKEMSECIRGRVDTLPESYRRVLVLSELVGMTTAEIASALGVSEGAVK
                     IRLHRARALLRQDLGTHCTLYHDELDGLACEPKPTFGSI"
     gene            2065..>2281
                     /locus_tag="VFK23_07785"
     CDS             2065..>2281
                     /locus_tag="VFK23_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7319017.1"
                     /translation="MAIDNKTKELIAVGASITANCQPCLQYHKSKALEYGADAGEIAE
                     AIEMGKQVRKGAANKMDEFSAGSKDCGF"
BASE COUNT          489 a          603 c          749 g          440 t
ORIGIN      
        1 tccttgctga gcgtgtcgcg acgatgaagc aggatgcccg gatggcgtac gagcggatga
       61 gggataccgc ggcgtccgac gaggcgcgat acgcgaagga gaaggagcag atcctcgaac
      121 aggccaaggc gctcgagaag gagcgcgacg tcagccgcgc gaaggatccg tatttcgatt
      181 tcggggaggt cctgctgcag ctcgcgatcg tcaccgcgtc gatcgcgatc ctgtccgggt
      241 cgtcccccgt ctaccttttc tcgattgtca tggcgacgct cggcgtggcg ttgtcgttga
      301 acggtttcct gctgctggtc acccttccgt tcctgactcc gtgactcgac ctgctccctg
      361 gtttcaggcc aggtgcatga caggtattga caattaatca gtgtgggtat acatttcagc
      421 atgtactgaa ataacagata aatgatttat gcctgatccg gggacggagg aggagcccaa
      481 aatgaaaaaa cgccaaagaa taagtcctgc tgcggcgggc cgttgatggg tgatgtgctc
      541 acgaaaatct gaaggagggg tggagatgaa gaccgaagat atccggaagt acgtgaggga
      601 tcgatatgcc ggcatcgcga aacagggagg ttcctgctgc ggcccatccg catgctgcgg
      661 cgcccccgag gcggcggaag aagcgagcgg ccggatcggt tatacggaag aggaaatgaa
      721 atcggcaccc cgggagtcca acctgggact cggctgcggc aacccgacag cgcttgcctc
      781 cctgcgggaa ggggagaccg ttctggacct gggctctgga gcgggcttcg actgcttcct
      841 ggcggcgaaa caggtcggtc ccggcgggaa agtcattggc gtcgacatga ccccggagat
      901 gattcaaaag gcccgcgaga acgcccgaaa gggcaaccat gaaaatgtcg aattccggtt
      961 gggggagatc gagcatctgc cggcggccaa tgactccgtg gacatcgtca tctccaactg
     1021 cgtcatcaac ctttctccgg ccaaggacaa ggttttcgcc gaggcctgcc gggttctcaa
     1081 gccgggaggc agggcaatga tctcggacat cgtcctttcc ggggatctgc cggaagccgt
     1141 ggccggttcc ctggccgctt atgccggatg catcgccggg gcgatccgga aggaggaata
     1201 tctccggctc atggaagcag cggggttccg ggacgtccat gtcgtccagg agagccgatt
     1261 tgaaattgcg gaaaccgaga ttgacgcgta tgcccggagc attgcaggga atttcaacgt
     1321 ccccctggaa gcggtccggg aggcagccag gtcggtcttg agcgtgcagg tcttcgggag
     1381 aaaaccggcg taggaggatg ccttgatgga cttcaagaga tctttccagg agatctacga
     1441 gaagttccac ccggggatcc ttcgatttct ggctcgtctt gtgggcgagg aggaggccga
     1501 agacgtggcc caagaggtct tcctccgggt ggatcggggg cttgagggct tccggggaga
     1561 gtcctccatg gggacctgga tttacaggat agcccggaac gctgcgctcg accgcctccg
     1621 cagccgaccg gcatggcttg agagcgtgca ccaactccaa acgcatgatt cggacgaaaa
     1681 cccgtcggat gtaatgtccc aaattccgga cgaacgggcg tcgctcgagc ggtatctgat
     1741 cgggaaagag atgagtgaat gcatccgggg acgtgtcgat acccttccgg agtcctaccg
     1801 gagagtcctg gtcttgagcg aattggtcgg gatgacgacc gccgagattg cttccgcgct
     1861 tggcgtaagc gagggggcgg taaaaatccg cctccatcgg gccagagcgc tgctgcggca
     1921 ggacttgggt acccactgca ccttgtatca tgatgaactc gacgggcttg cctgcgagcc
     1981 gaagcccacc ttcggttcga tctgactgta tcttttttcc ggacgtcttc gtctataggg
     2041 tcaaaaaccg ggaggtgatt actcatggca atcgacaaca agacaaagga actcattgcg
     2101 gtgggggctt cgattaccgc aaactgccaa ccctgtctgc agtaccacaa gagcaaggct
     2161 cttgaatacg gtgcggatgc gggggaaatc gccgaggcga tcgagatggg aaagcaggtc
     2221 cggaaggggg cggcgaacaa gatggatgaa ttctccgctg gttcgaaaga ctgcggattt
     2281 c
//
LOCUS       DASSLD010000429         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_262309,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000429 DASSLD010000000
VERSION     DASSLD010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262309"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFK23_07790"
     CDS             <1..477
                     /locus_tag="VFK23_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319018.1"
                     /translation="FVVVLMATYIPLNPIETYGPPSPGTPVMRPDWYFLVVYGFLKLV
                     PGDLVFTVLGGKITPETVGGVIFPALNFLAYALVPFFDRAKEPQHYMEDPLRRPFMTS
                     LGVGAIVFLCMMVVAGYIDVLHITPKAMTVYALVATAAAWGISYALLRAYNRKRGH"
     gene            477..1226
                     /locus_tag="VFK23_07795"
     CDS             477..1226
                     /locus_tag="VFK23_07795"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319019.1"
                     /translation="MPQRARVILCALLLCWTFAVPSHGADDGISGNSCVRCHSRLPAS
                     SFVGAKSHGWTGSIHQKHGVTCDKCHGGNPLAQEQNGAHAGVLGSSNPGSPVYFKNIP
                     STCGKCHGAEFYKFSQSRHYKILQSGGKGPECVTCHGSMVTGVLTPDTVAAVCQQCHN
                     ERMGTFPYVPQKAKAVLLLLTESATLLDAETKFYHPAAGTEPARALHDARSSLHSARL
                     NWHKFDLDTIIADLQEAYDSLKKLSPGSPGR"
     gene            1397..1912
                     /locus_tag="VFK23_07800"
     CDS             1397..1912
                     /locus_tag="VFK23_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET7319020.1"
                     /translation="MKLSWLIGGFIAAAAVLVAVLAFMYILPLDQRDRSPVQPIAFSH
                     KIHAGDNGIPCLFCHRYAPKSPVAGIPAVADCRACHLFIAQNTPEIKKLMTYWDEKKP
                     IPWVRVYGVPDHVYFPHMMHIRAGLACTTCHGNVAAMQRVTRSVKIRMGWCLGCHKEH
                     KASHDCWTCHK"
BASE COUNT          423 a          631 c          590 g          458 t
ORIGIN      
        1 ttcgtggtcg tgctgatggc gacctatatc ccgcttaatc ccatagaaac ctacggccct
       61 ccgtcgccgg gaacgccagt gatgagacct gactggtatt ttctcgtcgt ctacggattt
      121 ctgaagctcg tgcccggcga cctcgtgttt accgtcctgg gagggaagat cacgcccgag
      181 accgtcggcg gcgtaatctt ccccgcgttg aattttctcg cctatgcact ggttcccttt
      241 ttcgacaggg cgaaagagcc gcagcactat atggaagatc cgctccgccg gccgttcatg
      301 acctcgctcg gcgtcggggc gattgtcttc ctgtgcatga tggtcgtcgc cggatacatt
      361 gacgtgctcc atatcacgcc gaaggcaatg acggtctatg cgcttgtcgc gacagctgct
      421 gcctggggca tcagctacgc gctcctgcgc gcgtacaata gaaagagagg ccattaatgc
      481 cacagcgtgc tcgcgtcatt ttgtgcgcct tgcttttgtg ctggacgttc gccgtcccgt
      541 cgcacggcgc cgatgacggc atatcgggca attcctgcgt ccggtgccat tcccggcttc
      601 cggccagcag ttttgtcgga gcaaaatccc acggctggac agggtcgatt catcagaaac
      661 acggagttac ctgcgacaaa tgccatggcg gcaatcccct cgcgcaggaa cagaacgggg
      721 ctcacgcggg cgtgctcggc tcaagcaatc ccgggagccc ggtgtacttc aagaatattc
      781 cctccacgtg cggcaaatgc cacggagccg agttttataa attcagccag agccggcatt
      841 acaagatcct gcagtcggga gggaaagggc ctgaatgcgt aacctgtcac ggctccatgg
      901 tcaccggcgt gctgacgccg gataccgtcg ccgccgtatg ccagcagtgt cacaatgagc
      961 ggatgggcac ctttccctat gtaccccaga aagcgaaggc ggtacttctt cttctgacgg
     1021 agagcgcaac ccttctcgac gcggagacga agttttatca tcctgccgca ggcaccgaac
     1081 cggcccgggc gctgcacgat gcgcggtcgt cccttcattc cgcgaggctc aattggcaca
     1141 agtttgattt ggacaccatc atagctgatt tgcaggaggc gtatgattct ctgaagaagc
     1201 tgtcgccggg cagccccggc cggtgaattt cgcgattgca tccttcctgc gcgcaacaaa
     1261 ttacgtgacc ggcacctgct ctctgttaag ggacgcagcc atgtttcgct gtccgacttg
     1321 actctcctct catctcgggt aaacttggta gtatatcgcg tcctgcgaga ccccggctgg
     1381 agaaaggctt tatcaggtga aactatcatg gcttatcggc gggtttattg cagctgcggc
     1441 cgtcctagtt gcggtcctgg cgttcatgta cattctgccg ctcgaccagc gggaccgctc
     1501 tccggttcag ccgatcgcct tcagtcacaa aatccatgcg ggggacaacg gcatcccctg
     1561 ccttttctgc caccgctacg ctccgaaatc ccccgttgcc ggcatccccg cggtagcgga
     1621 ctgccgggct tgtcatctgt tcattgccca gaacactccg gaaataaaaa agctcatgac
     1681 atactgggac gaaaaaaagc ccattccctg ggtcagggtc tacggggtgc ctgaccacgt
     1741 ctacttcccg cacatgatgc atatccgggc cgggctggcc tgcacgacct gtcacggcaa
     1801 cgtggccgcc atgcagcgag tcacccggtc agtgaagatc aggatgggct ggtgcctggg
     1861 ctgtcacaaa gagcacaagg cgagccacga ttgctggacg tgccataaat gacacctcct
     1921 taaaacaatc ataacgtgtc accctgagcg aagcgaaggg gcttgcggag ggaagaaacc
     1981 cagattcttc gctgcgctca gaatgacaac gtagttgttg gagactttta tattttcagg
     2041 agtcccgatg gaccgaagga cctttctgca gcttagcgga ctgacggctg ccggcagcct
     2101 gc
//
LOCUS       DASSLD010000430         2983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_429219,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000430 DASSLD010000000
VERSION     DASSLD010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429219"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..698)
                     /locus_tag="VFK23_07805"
     CDS             complement(111..698)
                     /locus_tag="VFK23_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7319021.1"
                     /translation="MGNNSEYCNPPSAEDLRSALREMQVYVDVTEEDLQKIYEIALRL
                     ARERVAAHTPVRDVMTRDVITIKSTADLHEAAKILSDHRISGMPVVDDNNRVIGVISE
                     ADILTLAGMSREHTFKDILRNLLGEPVPARKTGNTVGDVMSFPPITSKGDDEIGDVAK
                     IFDERRIKRLPVVDDEGRLIGIVSRADIVRAIGRK"
     gene            complement(855..1751)
                     /locus_tag="VFK23_07810"
     CDS             complement(855..1751)
                     /locus_tag="VFK23_07810"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HET7319022.1"
                     /translation="MRSFREIKHNYRFTEEDEQRLAALRPLMEEHVDEVMGTLSLWIM
                     GTAGAAKFFTEETRQRHVFGAQREWFLDLFSGKYDNRYYEKLIRIGAVHVQHNVDAHY
                     MNRAVNLVKNACIGILQGLDEEKRETTNKIVSVGKILDISLDVITTSYIEEEIRTYSP
                     VYKVKSALISFSERFSQTTNLMLVLALIGLTIGVVWLFVQDVMHLLSGDVERGIISAL
                     GSMLLLWLMIELMNTEISHLKGGKFHISVFVGVALVTMIRETMIATLKHENPESIYYL
                     IAAILVIGFVYWIVTRAEEKHK"
     gene            complement(1771..>2983)
                     /locus_tag="VFK23_07815"
     CDS             complement(1771..>2983)
                     /locus_tag="VFK23_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319023.1"
                     /translation="GTLQSPRPGASGYKTVKARTLTEAIHVTHAASIPMPDKGGRTQN
                     CQVCHPSHWQQEAMNSGDNPFRIVDEGGNPRFSNADVRAAGGGCYLRRDAHANPAVKP
                     PFFLNAIGKWYLKEVSRKDENGITISELRGLYCTNCHSRLSQELYRHDDLTNAAEQEG
                     RTLRNRPVKDIMAEIAKKDKIRFKNYFADPIVGAGGDPLRSYYDKHEGAVLVRASKDE
                     TGKLKLLAWNAMAGNAVPYESASGGKDWWLSAAEPHCADCHLAPFVESAGGGYFPIDQ
                     PNKYSLYRYSKAHGVIACQSCHESVHGLYPVRYEGPETTVDLTTHEQALQFSPDGKYA
                     GPVTCGACHRVNDKGVPVQLSGTEYYQDYWASVVLLHAMRDGDQGLPVRKLVEKYPYK
                     KAQKIVQDGWE"
BASE COUNT          529 a          897 c          843 g          714 t
ORIGIN      
        1 ggtgtggcct tgacatgacc agattcttcg ctgcgctcag aatgacagcc cttgcttttc
       61 gaatgcatca ggttcattat tccgcattcc ccattccgaa ttccgcattg ctacttcctc
      121 ccaatcgccc gcacgatgtc cgcccgggag acgattccga tcaaccgccc ctcgtcatcg
      181 acaacgggca gtcgttttat cctgcgctcg tcgaatatct ttgccacgtc tccgatttcg
      241 tcgtcgccct tgctggtgat gggaggaaag ctcatgacgt cccctaccgt gtttcccgtc
      301 ttccgtgcag gcacgggctc cccgagcagg ttgcgcagga tgtccttgaa ggtgtgctcc
      361 cgggacatgc cggcaagcgt cagtatgtcc gcctcgctga tgacgccgat gactctgttg
      421 ttgtcgtcta cgacgggcat gccgctgatc ctgtggtccg aaagaatctt cgccgcttcg
      481 tgcagatcgg ccgtgctctt gattgttatg acgtcccggg tcatgacgtc cctgaccggc
      541 gtgtgcgccg caacccgttc ccgtgcgagc cgcagcgcga tctcgtagat tttctgcagg
      601 tcctcttcgg tcacgtcgac gtatacctgc atctccctga gggcagaccg caggtcttcc
      661 gcggacggag ggttgcagta ttcggaatta ttgcccatga gacctcactt gtggatgaat
      721 gataacagcg ccggcgactt cccggtatga tgaagatcat atcaaggttc tttactgtca
      781 gtagtcggct ttcggcaatc ggcttgcggc tctaatctga ccgctaactg ctgaccgctg
      841 aaagctgaat tcccctactt atgcttctct tccgcccggg tcacgatcca gtagacgaag
      901 ccgatgacga ggatggctgc gatcaggtaa taaatggatt ccgggttctc gtgtttgagg
      961 gtagcaatca tggtttcccg gatcatggtg accagggcca ccccgacgaa gacgctgata
     1021 tggaacttgc cgcctttgag gtgtgatatc tcggtgttca tcaactcgat catgagccac
     1081 aggagcagca tggaaccgag cgccgaaata atgccccgct ccacgtcgcc ggagaggagg
     1141 tgcatcacgt cctgcacgaa gagccacacg acgccgatcg taagaccgat gagcgccaga
     1201 accagcatga ggttcgtcgt ctgagaaaat cgctcggaaa aactgatgag ggcgcttttc
     1261 actttataga ccggcgagta ggtccttatc tcttcttcga tataggaggt cgtgatgacg
     1321 tcgaggctga tgtcgaggat ctttccgacg gaaacgatct tgttcgtggt ctcgcgtttt
     1381 tcctcgtcca agccctggag gatcccgatg caggcgttct tgacgaggtt gacggccctg
     1441 ttcatgtaat gggcgtccac gttgtgctgc acatgcactg cgccgatcct gatcagcttt
     1501 tcatagtacc ggttgtcgta cttcccggaa aaaagatcga ggaaccattc ccgctgggcc
     1561 ccgaacacgt gtctctgtct ggtttcctcg gtgaaaaatt tcgccgcgcc cgctgtcccc
     1621 atgatccaga ggctcagcgt tcccatgacc tcgtcgacgt gctcctccat gagcggccgc
     1681 agggcggcca agcgctgctc gtcttcctcg gtgaaccggt agttatgttt gatctctctg
     1741 aacgacctca tcgcgtattt ctcccgggct tcattcccag ccgtcctgga cgatcttctg
     1801 ggccttcttg tacgggtatt tctcaacgag ctttcggacc ggcagcccct gatcgccgtc
     1861 acgcatggcg tgaagcagca cgaccgacgc ccagtagtcc tggtagtatt ccgtgcccga
     1921 aagctgaacg gggaccccct tgtcgttcac ccggtgacag gcgccgcagg tcaccggtcc
     1981 cgcgtacttt ccgtcggggc tgaactggag cgcctgctcg tgcgtcgtca ggtcgacggt
     2041 ggtttccggt ccttcatacc gcaccgggta gaggccgtgg acggactcgt ggcacgactg
     2101 gcaggcgatg acgccgtgcg ccttggaata gcggtagagc gaatacttgt tcggctggtc
     2161 gatggggaaa tatccgcccc cggcgctttc cacgaaagga gcgagatggc agtccgcgca
     2221 gtgcggctcc gcagccgaca gccaccagtc tttcccgccc gaagcgcttt cataggggac
     2281 ggcgtttccc gccatcgcgt tccaggcgag aagcttgagc ttgccagttt cgtctttcga
     2341 cgccctcacg aggaccgccc cctcgtgttt atcataataa gaccggagcg ggtctccccc
     2401 ggccccgacg atggggtcgg cgaaatagtt cttgaacctg atcttgtctt ttttcgcgat
     2461 ctcggccata atgtccttga ccggcctgtt ccgcagagtc ctgccttcct gctctgcggc
     2521 attcgtgagg tcgtcatggc ggtaaagctc ctggctgagg cggctgtggc agttggtaca
     2581 gtagaggccg cgcagctccg aaatggttat cccattctcg tccttcctgc tcacctcttt
     2641 cagataccac ttcccgatcg cattcagaaa aaaaggcggc ttgacggcgg ggttcgcatg
     2701 tgcgtccctt cggagatagc atcctccgcc agcggccctc acgtcggcat tcgaaaaacg
     2761 ggggttgccc ccttcatcta cgatccggaa cggattgtcc ccgctgttca tcgcttcctg
     2821 ctgccagtgg cttggatggc agacctggca gttctgcgtt ctccctccct tgtccggcat
     2881 ggggatggaa gcggcatgag tcacgtggat agcttcggtg agcgtccttg ccttcaccgt
     2941 cttgtatccg gaagcgcccg gacgtggaga ttggagggtc ccg
//
LOCUS       DASSLD010000431         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_734217,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000431 DASSLD010000000
VERSION     DASSLD010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734217"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFK23_07820"
     CDS             complement(<1..281)
                     /locus_tag="VFK23_07820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HET7319024.1"
                     /translation="MNDAVTQLFILFGILIVVLTIGGLLIWLERRLLGLWQDRYGPNR
                     VGPFGLLQVVADMIKVFTKEDWIPPFADRSVFIIAPAVIVVVVLMSFTV"
     gene            complement(274..>2508)
                     /gene="nuoG"
                     /locus_tag="VFK23_07825"
     CDS             complement(274..>2508)
                     /gene="nuoG"
                     /locus_tag="VFK23_07825"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HET7319025.1"
                     /translation="DCPVCDEGGECHLQDMTVMTGHNYRRTRFRKRTHRNQDLGPCIN
                     HEMNRCIACYRCVRFYRDYAGGRDLDAFASHDNVYFGKAEEGALESEFSGNLVEVCPT
                     GVFTDKTLKRHYTRKWDLQTAPSLCVHCGLGCNTIPGERYGTLRRVLNRYNRQVNGYF
                     LCDRGRFGYEFVNSGRRIRKPLVRETRQSEQQPVEKDRALKHIHHIFAKSKRIIGIGS
                     PRASLESNFALRSFVGPDNFYSGMSPKEHGLIMLMIDILQKGPARTPSLHEVEMADAV
                     LVLGEDLTNTAPLLDLAVRQSVRNKPRELTDKMRIPAWDDNAVRSALQDETGPLFIAS
                     ITDTKLDEVSAKTFHAAPDDLARLGFAVARELNPEAPDVPDLPDDHRRLAKEIADALK
                     TAKRPLVISGASLGSASVIRAAANIAWSLCESGKPAELCFTVPECNSIGAGLLGGRNF
                     GELLHSSPAQPEGSIDAVIILENDLYRRTDKKSVDDFLDRAKHIIAIDHLIHPTSVRA
                     DVVLPAGTFAESNGTLVNNESRAQRYYQVFVPGLDIRDSWKWIEDIMTAVGKSAAGSR
                     RSLDDLINEISEALPVFSPVRDIAPSADFRISGQKVPRQPHRYSGRTSMLANINVHEP
                     KPVDDPDSPLSFSMEGYESRPPSPLIPRYWSPGWNSVQALNKFQQEVGGPLSGGDPGK
                     RLIEPAPDGRPPYFSDIPHAGAKDYSPADFPRYFIFGSEELSSYAPAIAERQKLRQGT
                     GNNE"
BASE COUNT          492 a          724 c          753 g          539 t
ORIGIN      
        1 atgacggtga aggacataag cacgaccacg acgatcaccg cgggcgctat gatgaagacg
       61 gacctgtccg caaaaggtgg tatccagtct tccttggtga agaccttgat catgtccgct
      121 actacttgaa ggagcccgaa aggccccacg cggttggggc cgtaccggtc ctgccacagc
      181 cccagcagcc tgcgttcgag ccagatcagc agtcccccta ttgtcaagac cacgatgagg
      241 atgccgaaga ggatgaaaag ctgtgtgacg gcgtcattca ttgtttcccg ttccttgtcg
      301 aagcttctgc cgctccgcta tggccggagc ataactgctg agttcctcgg aaccgaatat
      361 gaagtaccgg gggaaatcag caggcgagta gtcctttgct cccgcatgcg ggatgtcgct
      421 gaagtagggc ggtcttccgt ccggcgccgg ttcgatcagt ctctttcccg gatcgcctcc
      481 gcttaagggg ccgccgacct cctgctggaa cttattcagg gcctggacag aattccatcc
      541 cggcgaccag tagcggggaa tgagcggcga gggaggccgg ctttcatagc cttccatcga
      601 gaaggacaag ggagaatcgg gatcgtcaac gggcttcggc tcgtgcacgt taatgttcgc
      661 gagcatggac gtcctgccgc tgtaccggtg gggctggcgg gggaccttct gcccggatat
      721 ccggaaatcc gcggacggcg cgatgtccct gacgggggag aataccggca gagcttcgct
      781 tatttcgttt atcaaatcat ccaatgaccg ccgactgccg gctgccgact tcccgacggc
      841 cgtcatgata tcctcgatcc atttccagct gtccctgata tcaaggcccg gcacgaaaac
      901 ctgataatat ctctgcgcac ggctttcatt gttcaccaat gtgccgttcg actccgcgaa
      961 ggtcccggca gggagcacga catcggccct cacggaagta ggatgtatca gatggtcgat
     1021 ggcgataata tgcttcgccc tgtccagaaa gtcgtcgacg gattttttat ccgttctccg
     1081 atacaggtcg ttctccagaa tgatgactgc atcaatcgac ccctccggct gggcagggga
     1141 ggaatgtaac agctcgccga aattcctgcc gcccaagagg cctgcaccga tgctgttgca
     1201 ttccggaacg gtgaaacaca gttcggccgg ttttccggat tcgcagagcg accacgcaat
     1261 gttggccgcg gcccggatga ccgacgcgct gccgagagaa gcgccggaga tgacgagggg
     1321 ccgtttcgct gttttcaatg cgtcggctat ctccttcgcc agcctacgat gatcgtcggg
     1381 gagatcaggc acatccgggg cttccggatt caattcacga gcgacggcga aaccgagccg
     1441 tgcaagatca tcgggcgcgg catggaaggt ttttgcggag acctcgtcga gtttggtgtc
     1501 cgtgatcgat gcgatgaaca agggcccggt ttcgtcctgc agggcactcc tcacggcgtt
     1561 gtcgtcccac gccgggatac gcattttatc ggtcagctcc ctgggcttgt ttcgtacgga
     1621 ctggcgcact gccaggtcga gcagaggggc ggtattcgtg agatcttcgc cgaggacgag
     1681 caccgcgtcc gccatttcca cctcgtgaag ggaaggagta cgggcggggc ctttctgcag
     1741 gatgtcgatc atgagcatga tgagcccgtg ttccttcggc gacatgcccg aataaaaatt
     1801 atcaggcccc acgaaagacc gcagggcaaa attcgattcg agcgacgcgc ggggagaacc
     1861 gatgcctatg atgcgcttgc tttttgcgaa gatatggtga atatgcttga gcgcccggtc
     1921 cttttcaacc ggctgctgct cggactgcct ggtctcccgc acaagcggtt tcctgattct
     1981 tctgccgcta ttcacgaact cgtatccgaa ccggccccgg tcgcagagga agtatccgtt
     2041 cacctgtcgg ttgtaacggt tcaggaccct tcgaagcgta ccgtaccgtt cgccggggat
     2101 ggtattacaa ccgaggccgc agtggacgca aagggagggc gcggtctgga ggtcccactt
     2161 gcgcgtgtag tgacgtttga gcgtcttgtc cgtgaatacg cccgtgggac acacctcgac
     2221 gaggtttccg ctgaactcgc tttcgagcgc accttcctcg gctttcccga agtagacatt
     2281 gtcatgcgag gcgaatgcat caaggtcacg gccgcccgcg tagtcccgat agaacctgac
     2341 gcagcggtag caggcaatgc agcggttcat ctcgtggttg atacaggggc cgaggtcctg
     2401 gttccggtgc gtccgtttcc tgaatcgtgt ccttctataa ttatggccgg tcatcaccgt
     2461 catgtcctgg agatggcact cgccgccctc gtcgcagacc gggcagtc
//
LOCUS       DASSLD010000432         4723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1013169, whole genome shotgun sequence.
ACCESSION   DASSLD010000432 DASSLD010000000
VERSION     DASSLD010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013169"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VFK23_07830"
     CDS             complement(<1..790)
                     /locus_tag="VFK23_07830"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7319026.1"
                     /translation="MPDDAIRTALEYFNSNKTECLADLKDLIRIPSVSFPGFEAAPVR
                     QCAEAVAELLRKSGLTNVHLLESGEGYPSVFGEWTGAPGKQTILLYAHYDVQPVGREE
                     LWTTPPFEPAERGGRLYGRGSSDDKAGVSMLAVAVRSFLKSAGTLPVNVKVLIEGEEE
                     VGSSNINEILKQNLGLLSADSLLIADSGNFDSGVPALTASLRGIVTVNVEVRSLTSSV
                     HSGVWGGPVPDPVIALSKMIAGIADDEGRPAVPGILDKVRKLSPE"
     gene            complement(979..1974)
                     /gene="trpS"
                     /locus_tag="VFK23_07835"
     CDS             complement(979..1974)
                     /gene="trpS"
                     /locus_tag="VFK23_07835"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET7319027.1"
                     /translation="MKKRILTGDRPTGPLHLGHYVGSLRNRVELQHEYDTYILIADMQ
                     ALTDNADNPEKVRRNILEVALDYLAVGVDPGKATIVIQSLVPEIPELTMYYLNLVTVS
                     RLQRNPTVKEEMKQKGFEGNVPAGFLTYPVSQAADITAFQADLVPVGEDQLPMIEQTA
                     EIVKKFNNTYGPVLVEPRALVPKVSRLPGTDGQSKMGKSLGNAIFLSDPPETVAKKVK
                     TMYTDPGHIHAEDPGSVEGNPVFSYLDAFDPDSGTVEDLKRRYRAGGLGDVAVKKRLA
                     EVLENFLGPIRRKRAEYSKDPAHVMDILRDGTERGRAVASQTLALVRKAMRLDYF"
     gene            2044..2580
                     /locus_tag="VFK23_07840"
     CDS             2044..2580
                     /locus_tag="VFK23_07840"
                     /inference="COORDINATES: protein motif:HMM:NF010248.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HET7319028.1"
                     /translation="MNQARKNILITGLPGVGKTTAVIRLAEALRNFHPAGFYTSEIRD
                     ASVRQGFELVSFDGQRALLSHVTMKGPYRVGRYGVDIRGFEDFLGALDFAGRERGLVI
                     IDEIGKMECYSERFTKLVKDLLDSGKPMIATIALKSEGFISEVKKRGDVDLFELTQAN
                     RTTLAGELAHSLKTLLER"
     gene            complement(2612..4138)
                     /locus_tag="VFK23_07845"
     CDS             complement(2612..4138)
                     /locus_tag="VFK23_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HET7319029.1"
                     /translation="METKPLVLPEDENNKTLVANVHPPEWKNPEPASRYNLVVIGAGT
                     AGLVTAAGAAGLGAKVALIEQHLLGGDCLNVGCVPSKGIIRAARAVHDVRVSEEFGVH
                     GTGGAAIDFGKAMERMRRIRAGISFHDSAKRFSEELGVDVFIGRGRFTGPDSIDVDGK
                     RLRFAKAAVCTGARAAAPSIPGIEEAGYLTNENVFWLTELPRRLAVIGGGPIGCELAQ
                     AFARMGSEVTLFQRGGQVLPREDADAAESVRRSLIRDGVTLRLRTKSLAVKKKGKEKI
                     VILEEVGRTAEIAVDEILAGVGRAPNVEGLDLEKAGIVYDPREGVIVNDHLRSSNPRV
                     YAAGDVCSSFKFTHAADAMARIVIANALFQARQKASALVIPWCTYTDPEVAHVGTYEK
                     DAKAAGHDVLTLTAPLADVDRALLDGEAEGFARVHLKKGTDKILGATIVARHAGEMIN
                     ELSLAMTAGLGLSAIGRTIHPYPTQAEAIKKLADAYNRTRLTPFVKKLFSAWLTWQRS
                     "
     gene            complement(4215..4637)
                     /locus_tag="VFK23_07850"
     CDS             complement(4215..4637)
                     /locus_tag="VFK23_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET7319030.1"
                     /translation="MSEKVIIRQKNNYETTYQSLDPDEPGAKRPRPVLSISELTPYGM
                     VLAGLGTCTAFVVNTYAEHHGVHVDAVKITLGYGRSFKEDCEHCEEIDKYEDDIEMSV
                     SFEGDLTAEQREKLFKISLRCPVHKMLKSGIKVQSKPA"
BASE COUNT          891 a         1410 c         1398 g         1024 t
ORIGIN      
        1 cttccgggga cagcttccgc accttgtcga ggatgcccgg gacggcgggc ctgccctcgt
       61 cgtccgcgat gccggcaatc atcttcgaca gggcgatgac gggatcgggc acgggaccgc
      121 cccatacgcc ggaatggacg ctcgaggtaa gcgaacgcac ctcgacattg accgtcacga
      181 tgcctcggag cgacgcggtg agcgccggga cgccgctgtc gaagtttccg ctgtcggcga
      241 tgaggagcga gtccgccgaa aggaggccga ggttctgttt caggatctcg ttgatgttcg
      301 acgatcccac ctcctcctcg ccctcgatca ggaccttcac gttgacgggg agggtacccg
      361 cagacttgag aaaggaccgg accgcgacag cgagcatgga aactcccgcc ttatcgtcgg
      421 aggaaccgcg tccgtaaagc ctgccgccgc gttccgcggg ctcgaagggc ggcgtggtcc
      481 acagttcctc cctgcccact ggctgcacgt cgtaatgcgc atacagaagg atggtctgct
      541 ttcccggcgc accggtccac tcgccgaaaa cggacgggta accctcgccc gattccagaa
      601 ggtgcacatt ggtcaggccg cttttccgga gaagctccgc aacggcctct gcacactgcc
      661 tgaccggcgc ggcttcaaat cccggaaaac tgacgctcgg gatgcggatg aggtccttga
      721 ggtctgcaag gcattctgtt ttattgctgt tgaaatattc aagggcggtg cgtatcgcgt
      781 catcaggcat ggaaaccctc ccggatggtg tcttcgccgt ttcgataaaa aaaggctgac
      841 catgacgtca acccttttct ctattttacc cttgttcgtc cggattggaa ggggcaatag
      901 gcatcttgca ctctgtctcc gtccgctccc ggtttgtgaa tccggcgatt ttaggggacg
      961 gagatcccgg tgtcaagatc aaaaataatc gagccgcatt gccttccgga ccagggccag
     1021 cgtttgggag gccacggccc gcccccgttc agtcccgtcc cgcaggatgt ccatgacgtg
     1081 cgccgggtct ttggaatatt cagcgcgctt ccggcggatg ggccccagaa aattctcgag
     1141 cacctcggcg agcctttttt tcaccgccac atctccaaga ccccccgccc ggtagcgccg
     1201 tttcaggtcc tcaacggtcc ctgaatcggg atcaaaggcg tcgagataac tgaagaccgg
     1261 attgccctcg acactgccgg ggtcctcggc gtgtatatgc ccggggtccg tgtacatggt
     1321 cttcaccttt ttcgcgactg tttcaggagg gtccgacaaa aagatggcgt taccgagcga
     1381 tttccccatt ttactctgcc cgtcggtccc gggaagacgg gagactttcg ggaccagtgc
     1441 ccggggctcg accaggacgg ggccgtaggt attgttgaat tttttcacga tctccgcggt
     1501 ctgctcgatc atcggcaact gatcttcgcc cacggggacc aggtcggcct gaaaggcggt
     1561 gatgtcggcc gcctggctca cgggataggt caaaaaaccc gccggcacat tgccttcgaa
     1621 tcctttctgc ttcatttcct ccttgacggt ggggtttctc tggaggcggg aaacggtcac
     1681 cagattcagg taatacatcg tcagctccgg gatctccggc accagggact ggatcacgat
     1741 ggtcgctttc cccggatcca caccgacggc cagatagtcc agggcgactt ccaggatgtt
     1801 cctgcgaact ttttccgggt tgtctgcgtt gtccgtgagc gcctgcatgt cggcgataag
     1861 tatgtatgta tcgtattcat gctggagttc gacgcggttc cggagagatc ccacataatg
     1921 tcccagatga aggggacccg taggccgatc gccggtcaga attctttttt tcatgaaatt
     1981 tcctccttac tcctcatata ggtattgtag tacgatacgg caatcatcgc tataattact
     2041 tcaatgaacc aggcacggaa gaacattctc ataaccggtc tccccggggt tggaaagacg
     2101 actgccgtga tccggctggc cgaagcgctg cgaaattttc atccggcggg attctacacc
     2161 agcgagattc gtgatgcaag cgtgcgccag ggtttcgagc ttgtcagctt tgacggacaa
     2221 cgggcgctcc tttcccatgt gaccatgaag ggcccgtacc gggtcggaag atacggcgtg
     2281 gacatcaggg gctttgaaga cttcctcgga gcgctcgact tcgcaggcag agagagggga
     2341 ctcgtgataa tcgacgagat aggcaagatg gagtgttact cggaaaggtt cacaaagctc
     2401 gtgaaagacc ttctggattc cgggaaaccg atgatcgcca ccattgcgtt gaagagcgaa
     2461 ggttttattt ccgaagtcaa gaagagaggg gatgtggatc ttttcgaatt gacgcaggct
     2521 aatcggacaa cgctcgccgg ggaattggcg cactctctca agaccctcct ggaacggtga
     2581 tgaacccgcc tggccgtgct ctccgcgtct tttacgatcg ctgccaggta agccacgcgg
     2641 aaaagagctt ctttacgaac ggcgtaagcc tcgtccggtt gtaggcatcg gcgagtttct
     2701 tgatcgcctc ggcctgcgtg gggtaggggt ggatggtcct gccgatggct gagaggccca
     2761 gccctgcagt catggcgagc gagagctcgt tgatcatctc gcccgcgtga cgcgcaacga
     2821 tggtggcgcc gaggatcttg tcggtccctt ttttcagatg gacccgggca aaaccctcgg
     2881 cctccccgtc caggagcgcc cggtccacgt cggcaagcgg cgcggtgagc gtcagcacat
     2941 cgtggcctgc ggccttcgcg tccttttcgt atgttcccac atgggcgact tcggggtcgg
     3001 tgtaggtgca ccaggggatg acgagggcag aggccttctg ccttgcctga aacagcgcgt
     3061 tcgcgatcac gatgcgtgcc atcgcgtcgg ctgcatgggt gaatttgaag gaagaacaga
     3121 cgtctccggc ggcgtagacg cgggggttcg agctccggag atggtcgttc acgatgacgc
     3181 cctcgcgcgg atcgtagacg atgcccgcct tctcgaggtc gagcccttcc acgttcgggg
     3241 ccctgccgac gccggcgaga atctcatcaa cggcgatctc agcggtcctt ccgacctctt
     3301 caaggatgac gatcttctct ttcccttttt tctttaccgc gaggctcttc gttcgaagcc
     3361 tgagcgtaac tccgtctcgg atgagggaac ggcgcacgga ctctgcggca tcggcgtctt
     3421 cgcgggggag gacctgtccg ccgcgctgaa acagcgtgac ctcgctcccc attcgcgcaa
     3481 aggcctgggc cagttcgcag ccgatgggcc cgccgccgat gaccgcgagt ctacgcggga
     3541 gttccgtaag ccagaagacg ttctcattgg tgaggtatcc ggcctcctcg ataccaggga
     3601 tcgaaggggc ggcggcgcgg gcgccggtgc agacggcagc cttggcgaat cggagacgtt
     3661 tcccgtccac gtcgatgctg tccggacccg taaacctgcc ccggccgatg aacacgtcaa
     3721 cgcccagctc ttcggaaaaa cgcttcgcgg aatcatggaa actgatcccc gccctgatgc
     3781 ggcgcatccg ctccatggcc ttcccgaagt cgatcgcagc tccgccagtg ccgtgaacgc
     3841 cgaattcttc cgagacgcgg acgtcatgca cggcccgcgc agctctgatg atccccttgg
     3901 acggcacgca cccgacgttc aggcagtccc cgccgaggag atgttgctcg atgagcgcga
     3961 cctttgcccc gaggcccgcc gcccccgccg ccgtgacaag gccggcagtg cccgccccga
     4021 tcaccacgag gttgtagcgg gacgcgggct cggggttctt ccattcgggg ggatggacgt
     4081 tcgcgacgag cgtcttgttg ttctcgtcct cgggaagcac gaggggcttc gtttccatgg
     4141 atggatggcc tttcaattaa tggcgcggcg agcaatatgg tctgtgaata atccctcgca
     4201 catgaatcgg gcacttacgc cggcttggat tgtaccttga ttccgctctt caacatcttg
     4261 tgaacggggc accgcaaaga aatcttgaac agtttctccc gttgctccgc ggtgaggtcg
     4321 ccttcgaaac tcacggacat ttcgatatcg tcctcgtact tgtctatctc ctcgcaatgt
     4381 tcgcagtcct ctttaaatga ccgcccgtat cccagcgtga tcttcaccgc gtcaacgtga
     4441 acgccgtgat gctccgcata ggtattcacc acgaaagcgg tgcacgtccc gaggcctgcc
     4501 aggaccatgc cgtagggcgt cagctcgctg atgctcagta ccggacgggg ccttttcgct
     4561 cccggctcat cgggatcgag cgattggtac gtcgtttcgt aattattttt ctgacggatg
     4621 atgactttct cggacataca attcctcctt tgtcaggttc gcctatggtc aaaatttctc
     4681 cctcaaaaga tataagacga ggacgacacg gctttaaccg ctt
//
LOCUS       DASSLD010000433         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_608934,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000433 DASSLD010000000
VERSION     DASSLD010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608934"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..1776
                     /locus_tag="VFK23_07855"
     CDS             271..1776
                     /locus_tag="VFK23_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7319031.1"
                     /translation="MIIREHNWSLAANKQFDVIVIGAGVNGASIYNRLGSKGYKVLIL
                     DKGDFSCGTSQTSAMMIWGGLLYLRNLDISSVYSLSRDRDALIDSGDEQVSSRTMRYI
                     VSRKQGWNKYLVYSALHLYWLLGHCRRKRPGFEYDFEELNFLKRDGIHSSIVYQEGYL
                     RQSDSRFVLDGILSCQSEDSFALNYCAIREGDYNDHDKLWSLSLSDVLSNRQCSVKAK
                     VLLNCAGVWTDSVNEQFNIKTPYKHAFSKGVFIGFKRPDTHRFPLVFEMGEHGDAMAL
                     IPWGPVCLWGPTETIAKSIEEGYASNSDDIHFLLKHAARNLDSSLAGSDIISIRCGLR
                     PLVVKKDFEADCYPLELSRRSRVVEDSDLPWISVYGGKISGCISLAEAVAGKVSRRIP
                     QSLEDPKSPGCMRNEVRWSVFPGIDERVPAIDWCVENEFCCTLEDYLRRRTNISQWVP
                     RQGLGFHDEHVKTLEHLAFQLPASYAGSAADHLRKYVDKVRGDFDRLMKSI"
     gene            1793..>2184
                     /locus_tag="VFK23_07860"
     CDS             1793..>2184
                     /locus_tag="VFK23_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET7319032.1"
                     /translation="MKSHGHELPAVLGGPQAVTLDHASINKWPDIDREDEDAVLQVMR
                     DGNISTHPVIRQLEREYASFTGRSYALAHNNGTSALLAAFKSIGLQPGDEVLVPSATF
                     WASVLPMLWLGAVPVFCESEPDRMGIDPV"
BASE COUNT          511 a          579 c          614 g          480 t
ORIGIN      
        1 tcaacccctt ctactattac gacgaggaaa aggggaaggg ctcctattgg gcggttctcg
       61 gcgggttgat cggcatggag acgaccgcgg agcagaaaaa gaaactcagg ctgttctgga
      121 ttttttgatt atgctgtggc catgaatact tgcgaatatc acgacagcag ggatgagagc
      181 agcccaataa acaattgatc agaaaaaaaa ttaacctgaa cacggcatcg caacattcca
      241 gtcaattcac tgatttgccg ggacaaacac atgatcatcc gcgaacataa ctggtctctt
      301 gccgccaaca aacaattcga cgtcatcgtc atcggcgcag gcgtcaatgg cgcgagcatc
      361 tataataggt tgggctcgaa agggtacaag gtattgatcc tggacaaggg cgatttcagt
      421 tgcggaacga gtcaaacgtc ggcaatgatg atatggggag gcctgctgta tctgaggaat
      481 ctggatattt cgtcggtcta ctctctctcg agggacaggg acgcgctcat tgactccggt
      541 gacgaacagg tctcctcgag aaccatgcgg tacattgtga gccgcaaaca gggatggaat
      601 aaatatctcg tctattcagc ccttcacctc tactggctgc tcggccattg caggcgcaag
      661 aggcccgggt tcgagtacga ttttgaggaa ttgaactttt taaagcggga cggcatccat
      721 agttccatcg tctaccagga aggatacctg agacagtccg acagccgttt tgtccttgat
      781 gggatactgt cctgccagtc cgaggattca ttcgccctga attactgcgc catccgggaa
      841 ggggactaca atgatcatga taagctctgg tcactcagcc tgtcggatgt tctttcgaac
      901 cggcaatgca gcgtaaaagc aaaggtcctc ctgaactgcg ccggtgtttg gaccgacagc
      961 gtgaatgaac aattcaacat caagacccct tacaagcacg ctttcagcaa aggcgttttt
     1021 ataggcttta aacgcccgga cacgcaccgg ttccccctgg tcttcgagat gggtgagcat
     1081 ggagacgcca tggccctcat tccgtggggc cccgtatgct tatgggggcc cacggaaacc
     1141 attgcgaagt ccatcgagga aggatacgca tccaattctg acgatatcca ttttttattg
     1201 aagcacgccg cgagaaacct tgactcctcc ctggccggct ccgatatcat atccatccgg
     1261 tgcggcctcc ggcccctggt cgtcaagaag gactttgaag ccgattgtta cccactcgaa
     1321 ctctcccgtc gttcccgggt cgttgaggac tccgacctgc cgtggatctc ggtttacggg
     1381 ggcaagatca gcggatgcat ttccctggct gaagcagtgg ccgggaaagt ttcaagaagg
     1441 atcccgcaaa gccttgaaga tccgaagtcg cccggctgca tgagaaatga agtccgttgg
     1501 tccgtctttc ccggcatcga tgaaagagtc cccgcgatcg actggtgcgt tgaaaatgaa
     1561 ttctgctgca cgctggagga ttacctgaga cggcggacca atatttccca gtgggtgcca
     1621 aggcaggggt tgggttttca cgacgagcac gtgaagaccc tcgaacattt agcgtttcaa
     1681 ctccctgcat cttatgcggg atcggcggcg gaccacctgc ggaagtacgt cgacaaagtc
     1741 cgtggcgact tcgaccggct catgaagtca atctgaaacg ataggggggc ctatgaaatc
     1801 tcacgggcat gaactcccgg ctgttctcgg ggggcctcaa gcggtgacgc tggatcacgc
     1861 gtcgatcaat aagtggcccg atatcgaccg cgaagacgaa gacgccgtat tgcaggtcat
     1921 gcgggacggg aatatatcga cccatcccgt tatccggcag ttggagcggg aatacgcgtc
     1981 gttcaccggc cgttcctacg cgctcgcaca caataacggc acgtccgcct tactggcggc
     2041 cttcaagagt atcgggcttc agccgggcga cgaggtcctg gtcccatcgg caacgttctg
     2101 ggcatcggtg cttcccatgt tatggctggg ggccgtgccg gtgttctgtg aaagcgaacc
     2161 ggaccggatg ggaatcgacc cggt
//
LOCUS       DASSLD010000434         3863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_892889,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000434 DASSLD010000000
VERSION     DASSLD010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892889"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFK23_07865"
     CDS             <1..287
                     /locus_tag="VFK23_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319033.1"
                     /translation="MSADVEILVTTLKDVLSVPSQAVIEREGKKQVYVAEGSTLKSGD
                     KVVARLRPVEIGETNWSYTQIVSGIKHGEYVITTPEATGLKDKAKVKIEE"
     gene            392..1069
                     /locus_tag="VFK23_07870"
     CDS             392..1069
                     /locus_tag="VFK23_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7319034.1"
                     /translation="MIELSNITKIYRMGEVDITVLAGVCLSIKQGEFVAVMGPSGSGK
                     STLMNIIGCLDRPTSGTYRFEEREISSLSDDELASVRNVKIGFVFQTFNLLPRFSALK
                     NVEVPLIYSGVPARERRERAIPLLEKVGLGDRLHHKPTELSGGQQQRVAIARALINNP
                     PLLLADEPTGNLDSHSGEEILRILTGLNTLGVTIILVTHDQGVGARCKRMINLKDGQI
                     VGDRVCV"
     gene            1066..2274
                     /locus_tag="VFK23_07875"
     CDS             1066..2274
                     /locus_tag="VFK23_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7319035.1"
                     /translation="MNLPETFKVSLEAILSNKVRSSLTMLGVIIGVMAVILLVSIGEG
                     ARVYITKELTGLGTNLLIIQPGKTSASGGFHPPSAGTVRKLTYDDAQALRRRAWLLTD
                     AVPIVLGSGRIKYGNLGRDMMIIGSSPEFQRVRNLFVDTGSYVTQNDVDSKAKVVVIG
                     TKVKEELFGDENPLGKVVMLSDARYRVVGVMRKKGTSLGWDIDDVVFIPVTSGQELFD
                     TDSLFEIIASTPRAEDVDKATAQIKDILMKRHAHREDFTIQTQGAILSTMNTILGVLT
                     AVLGGIAGISLLVGGIGIMNIMLVSVRERTREIGIRKALGARNRDIMAQFMIEAMTLS
                     GAGGVIGILIGVGLALLIPLFIDVLPTSVSAWSIIMAFTFSAAVGVFFGVYPARKAAL
                     QDPIQALRYE"
     gene            2937..>3863
                     /locus_tag="VFK23_07880"
     CDS             2937..>3863
                     /locus_tag="VFK23_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7319036.1"
                     /translation="MATNAPNIAKITRPRIKNALPRERLFSLLDRSGSTPVMWVTGPG
                     GSGKTTLVASWLDARNLPCLWYQIDEGDVDMASFFYYLGMAAKKAAPRYRKPLPLLTP
                     EYLLGIPTFTRNYFENLYARLKPPCAVVFDNYHNAPEQSQLHDIVNTTMSHIPEGIEV
                     IVLSRSEPPVAFTRLQANGSMTLVGWNELRFTLEETKQLIELKGKWKPADHSVKELHA
                     RTEGWAAGLVLLLEQARQGDAGPELTGRLAELSREAVFDYFAGEIFNSLDSETTDFLL
                     MSSFLPKMTVHMAEKMTDQDNAGHILSILNRNN"
BASE COUNT          896 a         1058 c         1091 g          818 t
ORIGIN      
        1 gaatgtccgc tgatgtggaa atcctggtga caacgctgaa ggacgtcctg tccgtgccgt
       61 cccaggcggt cattgaacgg gaggggaaaa agcaggtcta tgtcgccgag ggcagcacgc
      121 tcaagtccgg tgacaaggtc gtcgccaggc tccggcccgt ggaaatcggt gagaccaact
      181 ggagctatac ccagatcgtc agcggcataa aacacgggga atacgtcatc acgacgccgg
      241 aggccacggg gctcaaagac aaggcgaagg tgaagataga ggaataagta caaagtacat
      301 agtacagggt acagagtaca gggttaatac gaatatcaag acaatattcc actttgtact
      361 atgtactttg tactctgtac tttttttgat tatgatcgag ctttccaaca tcaccaaaat
      421 ctaccgcatg ggagaggtgg acatcaccgt cctcgcaggt gtctgcctga gcataaaaca
      481 gggggaattc gtcgccgtca tgggcccctc gggatcaggc aagtcgaccc tcatgaacat
      541 catcggctgt cttgaccgtc ctacctccgg cacgtaccgt ttcgaggaac gggaaatcag
      601 ctcgctcagc gacgacgaac tggcatcggt caggaacgtt aagatcggtt tcgtgttcca
      661 gacattcaac ctgcttccgc ggttttccgc gctcaagaat gtcgaggtcc ctctgatcta
      721 cagcggggta cccgcgcggg agcggcggga acgggcgatc cccctgctcg aaaaagtagg
      781 gctcggcgac cggctgcacc acaaaccgac ggagctttcg gggggccagc agcagcgtgt
      841 ggccatcgcc cgggcgctga tcaacaaccc gcccctcttg cttgccgacg aacctacggg
      901 aaacctcgac agtcactccg gagaggaaat cctccggatt ctcaccggcc tgaacacgct
      961 gggcgtcacc atcattcttg tcacccatga tcagggcgtc ggcgcgaggt gcaagcggat
     1021 gatcaatctg aaggacggtc agattgtggg cgaccgggtg tgcgtatgaa cctgcccgag
     1081 acattcaaag tatcgctcga ggcgatcctg tcgaacaagg tccgttcaag cctcacgatg
     1141 ctcggcgtca ttatcggcgt catggcggtg atcctcctgg tgtccatagg ggagggcgcc
     1201 cgggtctata tcacgaagga actgaccggc ctcgggacga acctcctcat catacagccg
     1261 ggaaagacat cggcgagcgg gggcttccat ccgccgtcgg cgggcacggt gcggaagctc
     1321 acctacgacg acgcgcaggc cctccggcgg cgcgcgtggc ttctgaccga cgcggtgccg
     1381 atcgtgctcg ggagcggcag gatcaaatac ggcaacctcg gccgcgatat gatgatcatc
     1441 gggagctcgc cggaattcca gcgcgtccgc aacctcttcg tcgatacggg cagttacgtg
     1501 acgcaaaacg acgtggattc caaggccaag gttgtcgtca taggcacgaa agtaaaggaa
     1561 gaattgttcg gcgacgaaaa cccgctggga aaggtcgtga tgctttccga cgcgcgttac
     1621 cgcgtcgtgg gggtcatgcg gaagaagggg acgagcctcg gctgggacat cgatgatgtg
     1681 gtgttcatcc cggtaacgag cggccaggag ctattcgaca ctgattccct gtttgagatc
     1741 attgcctcga cgccccgggc ggaggacgtc gataaggcaa ctgcgcagat aaaggacatc
     1801 ctcatgaagc gacacgcgca cagggaggac ttcacgatcc agacccaggg ggccattctt
     1861 tccacgatga atacgattct gggagttctg acagcggtcc tgggaggcat cgccggcatc
     1921 tcgctcctcg tgggcggcat cggtatcatg aacatcatgc tggtctcggt acgggagcgc
     1981 acgcgggaga tcggcatacg gaaggccctg ggcgcgagga accgcgatat catggcccag
     2041 ttcatgatcg aagccatgac gctttcgggc gcgggaggcg tcatcgggat actgatcggt
     2101 gtgggcctgg cactcctcat cccgttgttc atcgacgtcc tgcccacgag cgtctccgca
     2161 tggtccatca tcatggcctt cacgttttcc gccgcggtcg gggtcttctt cggcgtgtat
     2221 ccagcgcgca aggccgccct ccaggacccg atccaggccc tccggtacga gtagacgcca
     2281 ttcgctgacg aaacgttaca aaaattcacg tcaaaatatc ttagaaaaag ccaatttcct
     2341 cttgaaatcg ataacggcct atgataatgt tttattgaca actattttta ccatgcccaa
     2401 cattattgtt ctttcctgat cctcatctca tgcgcgatca acaacatctt tgaaagtttg
     2461 cgtcaaggaa ggatcatccc tgatgcgtca acgcatggcc atagtgtgca tcgcaatcgt
     2521 ctgcctcgcc gtctgcgtgg tggcagccca ccatcacgac gggactgatt tcagcagaaa
     2581 gtgcgccatt tgctttgcgt caaaaatatc tttcgccgca gcaaacagta gtcctgatct
     2641 tttccagtac tggatcgcca caatatcttc tcccgaaact acgttgactc ctcctggatc
     2701 ttccgcaaca cctttcgcct ctcgcgctcc gcctgcatag aagtatttcg gttaatcaca
     2761 atagtaactt atggcctatt aaaatgccag aaagcgatct ttttgtcatt tcgagcgaag
     2821 cgagaaatcc cagagtccga aatctacata agatttctct tcggtcgaga tgacatcaag
     2881 aatgtctgag ggtttactga gtccgacgct ctgcatcgag ttcagggaaa tcgtcgatgg
     2941 cgaccaatgc tcctaatata gcgaagataa cccgcccgcg gatcaagaac gcgctgcccc
     3001 gggagaggtt gttctctctt ctggacagga gcggctctac gccggtcatg tgggtcacgg
     3061 gccccggcgg ttcaggcaaa acaacgctcg tcgcgagttg gctcgatgcg aggaatctgc
     3121 cttgcctgtg gtaccagatc gacgaaggtg acgtcgatat ggcgagcttt ttctattacc
     3181 tgggtatggc ggcgaaaaag gccgcgccac ggtatcgaaa accactgcct cttcttaccc
     3241 cggaatatct tctgggcatt ccgacgttta cccggaatta ttttgaaaat ctatatgcca
     3301 ggctgaagcc accttgtgca gtcgtattcg ataattacca caacgcaccc gaacaatcac
     3361 agctccatga tatcgtaaac acgacgatgt cgcacatccc cgaggggata gaggttatcg
     3421 tactgagcag gagcgagcca cctgtggcct ttacgcgtct ccaggcgaac ggttcgatga
     3481 cgctggtcgg atggaatgaa ctccggttta ccctggagga aacgaagcag ctcatcgagt
     3541 tgaaaggaaa gtggaagccg gccgatcatt ctgtgaaaga gcttcatgcg aggaccgaag
     3601 ggtgggcggc ggggttggtg cttctcttgg agcaggcaag acagggagat gcagggcctg
     3661 aactaacggg tagactcgcg gagctttccc gggaagcggt cttcgattat tttgcaggag
     3721 agatattcaa cagcttagat agcgagacta cggatttttt gctcatgtcc tcattcctgc
     3781 ccaagatgac cgtgcacatg gcggaaaaga tgaccgacca ggacaacgcc ggtcatatac
     3841 tcagtatctt gaaccggaat aac
//
LOCUS       DASSLD010000435         4017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_983771,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000435 DASSLD010000000
VERSION     DASSLD010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983771"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(555..1484)
                     /locus_tag="VFK23_07885"
     CDS             complement(555..1484)
                     /locus_tag="VFK23_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7319037.1"
                     /translation="MILIISLIIFVSACAGPLGDASKALKDWDYKTAYRLVKLWAGQR
                     SPESQLKLAIMYYKGEGVPQNYAEATKWLRKAAEQEHVIAQFLLSSMYSQGLGLRQDD
                     PKAAKWLRKAADKGDGYSQANLGLMYYKGIYYGGLGVPQDYAKAAKWYRKAADQGIAS
                     AQNNLGTMYDKGEGLAQDNAEAAKWYRKAADQGNAEAQNNLGPMYYGGLGVPQDYAEA
                     AKWYRKAAEQGNAKAQGSLGFIYLMGRGVPRDYVISHMWFNLAGARFSGSERKKREIV
                     EHYMDIDDSKMTPAQVAEAQKLAREWKPKKEQK"
     gene            1668..2237
                     /locus_tag="VFK23_07890"
     CDS             1668..2237
                     /locus_tag="VFK23_07890"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7319038.1"
                     /translation="MRRVLPIVRFSLIILLLTALACPALSEDINTLTKKAKDGDPEAQ
                     YNLGIALSSKDLPWPGDFQLAMEWLHKAAEQGHIKAQFALGNIFTEGKITPQDYAEGV
                     KWYRKAAEQGNVGAQYNLGLHYYDGKGVPQDYVLAYFWTSLSASRSTGDDYNKSVKYR
                     DEAAKKLTNEQLLKAQKMTREWEAKHPRK"
     gene            3318..3527
                     /locus_tag="VFK23_07895"
     CDS             3318..3527
                     /locus_tag="VFK23_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319039.1"
                     /translation="MEDAEKAAIQKTGKGLQDLLVGLKTEEDWQLERLEDVERLTNII
                     HRYRVRGARSQEIAWAIIEFVKKGD"
BASE COUNT          932 a         1062 c         1052 g          971 t
ORIGIN      
        1 catcgcctgc cattaagaat cccctcgccg atggccgcgt ccggggagca ttgccgatca
       61 tgggcttccc ccggagcgcg tccccctggt tcgatttcct catgctttga accaccgggt
      121 aaaaatcccg cctcccgtag ctccggggcg aactgtcgtt atgattaggc aggagcatta
      181 tgcctattgc cggttgttca cccgccttcc cccggcggag ctcacggttg gtgcgatcct
      241 aaatctcggg ctatcgcctt ctcgataagc gcactcgggg tgtaacggtt tattaggact
      301 gcccgtgtat tcccgcactg gcagtcccat agaattgcct tgagcagatg agtcccgatt
      361 ggatcgacta atgccagatc ttctatccgt ccgcatatcg agcagggggt tgacttggaa
      421 ccatggcggc ttacagtaat agcaaagaaa accatcgccg cgccgacgag accgagaccg
      481 agtaacagac gttcgcgcac tgtacattct ccttattcct gaagtccgaa aatgtaccgc
      541 ccgagcagtt cgggttactt ctgctccttc ttcggcttcc actccctcgc caacttctgc
      601 gcctcagcga cctgggccgg ggtcatcttg gagtcgtcaa tatccatgta atgttcgacg
      661 atctcccgct ttttacgctc tgatcctgaa aatcgcgctc ctgcaaggtt gaaccacata
      721 tgggagatta cgtagtcccg tggcaccccc ctacccatta aataaatgaa gccgaggctc
      781 ccctgggctt tggcatttcc ttgttctgcc gcttttcgat accatttcgc cgcctcggcg
      841 taatcctgtg gcacaccaag gcctccatag tacatggggc cgaggttgtt ctgggcttcg
      901 gcattacctt gatctgccgc ttttcgatac catttcgcgg cctcggcgtt atcctgcgcc
      961 aaaccttcac ctttgtcgta catggtgccg aggttgttct gggcagacgc aatcccctga
     1021 tctgccgcct tccgatacca cttcgccgcc ttagcgtaat cttgtggcac gccaaggcct
     1081 ccatagtaaa tgcctttata gtacatgagg ccgaggttgg cctgagaata cccatctccc
     1141 ttatctgccg ctttccgaag ccatttcgcc gctttggggt catcctgccg cagacctagg
     1201 ccttgggaat acatcgaact gaggagaaac tgggctatga cgtgttcctg ctcggccgcc
     1261 ttgcggagcc acttcgtcgc ctcggcatag ttctgcggta caccttcgcc tttgtagtac
     1321 atgatggcga gttttaactg ggattcgggt gatctctgcc cggcccatag tttgaccaag
     1381 cgataggcgg tcttgtagtc ccaatccttt aacgctttag aggcatctcc gagtggccca
     1441 gcgcatgccg acacaaaaat gattaaggag atgatcagga tcacggcggc ggcagttctt
     1501 cgcatatgcc acctcccaag tgaatcggat tcaccccgag tgtattccta agtgggactc
     1561 ccgacaatca agatcggggg gagggaccgc gtgaatccgc ccgctcccca ccgcttggtg
     1621 atatagtcct tggagactct tgccgcatcc tcttccccgg aggtcctatg cgtagagtcc
     1681 ttccaatcgt ccgcttctcc ctgatcatcc tcttgctgac cgccttggcc tgccccgcat
     1741 tatccgagga catcaatacg cttaccaaga aggcgaagga cggcgacccg gaggcacaat
     1801 acaaccttgg gattgcgtta tccagcaagg atttgccgtg gccaggggat ttccagttag
     1861 cgatggagtg gttacacaag gccgcagaac agggacatat aaaagcccag ttcgccctcg
     1921 ggaatatatt taccgaaggg aaaatcactc cacaggatta tgccgaaggg gtgaaatggt
     1981 atcgcaaggc cgcagaacaa ggaaatgtcg gtgcccagta caacctcggt ctccattact
     2041 atgacgggaa aggtgtccca caggactacg tgcttgcata cttctggaca tcattgtctg
     2101 catcgagaag cacaggtgat gattacaaca agagcgtgaa atatagagat gaggccgcca
     2161 agaagttgac taatgaacaa ctcctgaaag cccaaaagat gacccgagag tgggaggcga
     2221 agcatccgag gaagtgagtt gtccgaatca acattccgcc cccttcgctt tccccgataa
     2281 tgtccctgac acgcttgggg gcgctgccaa tggataatcg tcgatcacgg tttaccttcc
     2341 ttcttgtccc ccttcttatc tttatctttg cccaggcatc cctgtcacag ggccaagctc
     2401 ttcttcaaga tactaaccct aataatgaca agggtgcctg cggaatgtta aatggtatga
     2461 ttaataaatt tcaggctttg gtggtaacga acagcataat ccattctgaa gccgatgcgt
     2521 aaattacctt aatagatatg acgaaaaaaa ccttagggtg taaaaactga aaatttccat
     2581 taaatgacat attggatagt tctttagtaa atcgaataga ttgaagagaa ggggcggagc
     2641 cgcaaacccc gccctttctg tttccgcatt aacgagcgat tgtcaaagat cccagatcgt
     2701 cggcacgccc tcctcttccg cattccttgc cttttgaaat tttggagagc gaaagaacat
     2761 ctaatgtatg gttgaaaacc gcgacgtcat gcgaggaggt ggagcaactt gagaggccgg
     2821 aaaacgtgga aaagctccca gaaatatcgt gtatccgacg ggatcgccag ggtcatcatc
     2881 cagttcgtga agaagtgcga ggaagtgcga cacgggcggt tgatgataaa acgatagtgt
     2941 gcctacgaag gatgtggaaa aatgaaggcg tctgcggcag gggtggtttt ggcagccatc
     3001 ctgatgtttg gctaggtttc gcacctttca ttatgctcct atgggggagt ggaccggcgg
     3061 agttgggtgg gtacgccagg aggcggctta agaagttgcc gcccagcaga tagcgtgacg
     3121 agagggcggg gtaacccgcc ctttctgtta ccgcattaag gagcgattgt caaagatccc
     3181 agatcgtcgg caagccctcc tcttccgccg cccgtgcccc tggaaatttc aacaaatctt
     3241 gcaatttcgg aaagggcaag aacatctaag tggtgggtga aaactttgct gtcaccacgt
     3301 taacgaacgg ggggaccatg gaagacgcgg aaaaagctgc cattcaaaaa accggcaaag
     3361 gtttacagga cctcttggtc ggcttgaaaa cggaggagga ttggcagctt gagaggctgg
     3421 aggacgtgga gaggctaaca aatatcatcc ataggtatcg cgtaagagga gcgcgatccc
     3481 aagagatcgc ctgggccatt atcgagttcg tgaagaaggg tgattgaggg atgggtatgg
     3541 aagtagaaga atacgacgat accagcccct tttaatcccg tactcatagg cgattttcaa
     3601 agaccgtagt tataccgccg actccacgtg gggaagatct ccctgtgaga atagcaaacc
     3661 cttttaccgg ggagccatgg aagaagagga cgtcccgctc atgctctcct caacgaacgt
     3721 ccggatgctt gctgcactga cccttgccat gtaggttcct ccttcccgga tattggtggg
     3781 cgaccaaatc cagtttaggg ggagaccgac ttggtatttc tgattccgca ttccggtgga
     3841 tgtggatgtg cgccatcact ctgttagagt ggttccctct tctcccactg atgattggat
     3901 tcatatcgcc gggagcgttc gcgaggcggt agaagtgatc gtgcttcccg ctcacaattt
     3961 gttgccggga taatattcgt tcacttttat attcattgtg ttttatatta gatagtg
//
LOCUS       DASSLD010000436         5367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1600272, whole genome shotgun sequence.
ACCESSION   DASSLD010000436 DASSLD010000000
VERSION     DASSLD010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600272"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFK23_07900"
     CDS             <1..201
                     /locus_tag="VFK23_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7319040.1"
                     /translation="AVVLPRQVAMYLCRNLANSSLPEIGNFFGGKDHSTVIHACKVIE
                     DKKEKDPDLKARIEMLIRQIRH"
     gene            288..1403
                     /gene="dnaN"
                     /locus_tag="VFK23_07905"
     CDS             288..1403
                     /gene="dnaN"
                     /locus_tag="VFK23_07905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET7319041.1"
                     /translation="MKLTIKKEEFLKGLQRIQGVVEKKNTMPILSNMLLTAEGKSLEI
                     VATDLEIGLRGRYVAEVEKPGAVTVSAKKMYEIVRELPAEDVQIKVEEGSWVKIASGR
                     SQFKLVGLPKDEYPALPDVAEEGMIVIDGTTLRDMIRKTLYSVGENDARYVLNGLYVH
                     MSQARQGLTIRMVGTDGHRLSMIDRVVDAKHRDESIIIPKKAMLELRRLLEEEAPQAE
                     LRLGFSKNHALFKRDGLVMVSKLIDGNYPNYLQVLPTKNTKKVTVSKDSLTHAVKRVS
                     ILSKEKTNAVKLQLEKDRLMLSTNNPEVGEANEELGVEYGGEGITIGFNSRYLLDVLT
                     AMDRASVALELSDALSPCLITEDGDEHYKCVVMPMRV"
     gene            1536..3938
                     /gene="gyrB"
                     /locus_tag="VFK23_07910"
     CDS             1536..3938
                     /gene="gyrB"
                     /locus_tag="VFK23_07910"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET7319042.1"
                     /translation="MEYTADSIKVLEGLEAVRKRPAMYIGSTGSVGLHHLVYEIVDNS
                     VDEAMAGFCRNIDVIIHVDNSITVIDDGRGSPTEMHSIQKRSAAEVALTVLHAGGKFD
                     SDVYKVSGGLHGVGVSVVNALSEWLELEIKQAGKVFQQRYERGNPQAPLAVTGTTATT
                     GTKITFKPDFQIFEDREYSYDILSQRLRELAFLNRGLKISIADERSGKKQTFFYEGGI
                     VSFIEYLNKNKTSLHPAPIYLSKEREGIFVEIAFQYNDSYVEQVFSFANNINTQEGGT
                     HLVGFKAALTRTVNSYAVQSGLFKNVTEALSGEDVREGLTAVVSVRLQNPQFEGQTKT
                     KLGNSEVKGIVEAMVNESLGSYLEENPLVARKVAEKALNAARAREAARKARELTRRKG
                     VLDSTSLPGKLADCQEKDPALSELFIVEGDSAGGSAKQGRDRKNQAILPLKGKILNVE
                     KARFDKMLSSDEIKVLITALGAGIGPEEFDIAKVRYHKIVIMTDADVDGSHIRTLLLT
                     FFYRQMLQIIERGYLYIAQPPLFKIKRGKTERYIKDDSRMEDFLLDLAAEDMELFSPE
                     QNGYLTGERLTSTLKKLVAFEKNLDKFKRKRIDTVVLRAILLDKTFNKAVLKDGEKLK
                     TVAASIEGYLKHYHPKTVIDWAIEKDEEHEAHKLRFDTRWDAIVSSIVFDENFVTSPE
                     FREIQSLSPAMLGLDGPDYRIREKGEERTFSHTGKLVHYVLELGKKGLAIQRYKGLGE
                     MNPEQLWQTTMDPEKRTFLQVTIEDSVKADEIFTILMGDAVEPRRAFIQRHALEVKNL
                     DV"
     gene            4011..>5367
                     /locus_tag="VFK23_07915"
     CDS             4011..>5367
                     /locus_tag="VFK23_07915"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HET7319043.1"
                     /translation="MEPVELKSQINIEQEMKTAYLDYSMSVIVGRALPDVRDGLKPVH
                     RRILYAMFREGLLHNKKYSKSAGVVGEVLKKYHPHGDSAVYDTMVRMAQDFNMRYPLV
                     DGQGNFGSVDGDPAAAYRYTEARLTKLAEEMLSDIDKETVDFIPNFDDTIVEPSVLPT
                     RVPNLLINGSAGIAVGMATNIPPHNLSEVVDGIVMMIDNSEVSIPELMTAIKGPDFPT
                     AGFIHGYEGIKQAYTTGRGIIQMRARAMIEEGRGERESIIVTELPYQVNKARLLERIA
                     ELVQDKKITGIAEIRDESDREGLRIAIDLKKGEIASLTLNQLYKHTAMQSTFGVIMLA
                     LVNNQPRVLNLRKLLSNFIQHRREVVIRRTRYDLRKAEERAHILLGLKIALENLDAVI
                     ALIKASANPDEAKTGLVREFKLSEIQAQAILDMRLQRLTGLERDKIVAEYEEVLKEIA
                     RL"
BASE COUNT         1278 a         1533 c         1606 g          950 t
ORIGIN      
        1 gccgtcgtgc ttccccgcca ggtggcgatg tatctttgcc gcaatctcgc aaactcatct
       61 cttccggaaa tcggaaattt cttcggcggc aaggaccaca gcacggttat ccacgcctgc
      121 aaagtgatcg aggacaagaa ggaaaaagac cccgatctca aggccagaat cgagatgttg
      181 attaggcaga taaggcattg aaaataaggg ctaatatgaa caatattttt attgctattt
      241 aaacggtatt ctgttaaaat tttggatgtg acaggaggct tctccagatg aaactgacga
      301 tcaagaaaga agaattcctg aagggactgc agagaatcca gggcgttgtg gaaaaaaaga
      361 acaccatgcc catcctctcc aacatgctcc tcacagccga aggtaagagt ctcgagattg
      421 tcgccacgga ccttgaaatc ggacttcgcg gccgctatgt cgccgaggtc gaaaaacccg
      481 gggcggtgac cgtttccgcc aagaagatgt atgagatcgt ccgggagctc cccgccgagg
      541 atgtgcagat caaggttgag gaagggagct gggtcaagat cgcctcgggg cgttcgcagt
      601 tcaagctggt gggcctgccg aaggacgagt atcctgcgct tcccgacgtg gcggaagagg
      661 ggatgatcgt gattgacggc actaccctcc gggacatgat caggaagacc ctctattccg
      721 tcggagaaaa cgacgcgcgg tacgtgctga acggcctgta cgtgcacatg tcccaggcaa
      781 ggcaaggtct cacgatccgc atggtcggaa cggacggcca ccgcctttcc atgatcgacc
      841 gcgtcgttga tgcgaagcac agggatgaga gcattatcat ccccaagaaa gccatgctgg
      901 agctgcggcg gctcctcgag gaggaggctc cccaggcgga gctccggctg ggcttcagca
      961 agaaccatgc tctcttcaag cgcgacggac tcgtcatggt gtcgaagctc attgatggaa
     1021 attaccccaa ctacctccag gttctgccca cgaagaatac gaagaaagtg acggtttcca
     1081 aggattcgct cacccacgcg gtgaaacgcg tctcgatcct gtcgaaggaa aagacgaacg
     1141 ccgtgaagct gcagctcgag aaagaccgcc tcatgctttc cacgaacaac ccggaagtcg
     1201 gcgaagcgaa cgaggagctc ggcgtcgagt acggcggcga gggcatcacc atcgggttca
     1261 actcccggta cctcctggat gtgcttacgg ccatggaccg tgcaagcgtc gcgctcgagc
     1321 tcagcgacgc gctgagtccc tgtctcatca cggaagacgg cgacgagcat tacaagtgcg
     1381 ttgtcatgcc gatgcgtgtg tagcaagcac gaaaccataa aacaagttga attgagagca
     1441 ggtttagaaa tcagagtttc ggatttagta tttgtgatcg aggtgtgagg gatttccgcg
     1501 gtccgcggct aaatccctct ttttgtgagg accggatgga atatactgcc gacagcataa
     1561 aggtgctcga gggtctggaa gcggtcagga agcggccggc catgtatatc ggcagcaccg
     1621 gcagcgtcgg gctccatcac ctcgtctacg agatcgtgga caacagcgtg gacgaggcca
     1681 tggcggggtt ctgcaggaac atcgacgtca tcatccacgt ggacaatagc atcaccgtca
     1741 tcgacgacgg ccgcggcagt cccacagaga tgcacagcat acagaagcgc tccgccgccg
     1801 aagtggcctt gaccgtgctc cacgccggtg gcaagttcga cagcgacgtg tacaaggttt
     1861 cgggcgggct tcatggcgtg ggcgtatcgg tcgtgaacgc gctttcggag tggctcgaac
     1921 tcgagatcaa gcaggccggc aaggtgtttc agcagcgcta tgagcgcggc aacccccagg
     1981 cgccgctcgc cgtgaccggt acgactgcca ctacaggcac caagataacc ttcaagccgg
     2041 attttcaaat ctttgaagac cgcgaataca gttatgacat cctctcccag cggctccggg
     2101 aactcgcttt cctgaaccgc ggactcaaga tatcgatcgc cgacgaacgc tccgggaaaa
     2161 aacagacttt cttctatgag ggcggcatcg tttcgttcat cgagtacctg aacaagaaca
     2221 agacgtcgct tcacccggcg ccgatctatc tctcgaagga gcgcgaaggc atcttcgtcg
     2281 agatcgcgtt ccagtacaat gacagctacg tggagcaggt cttttccttc gcgaacaaca
     2341 tcaataccca ggaagggggc acgcacctgg tcgggttcaa ggcggcgctc acgcgcaccg
     2401 tgaacagcta cgccgtccag agcgggctct tcaagaacgt gaccgaggcg ctctcgggcg
     2461 aagacgtgcg cgagggcctg accgctgtcg tgagcgttcg cctgcagaac ccgcagttcg
     2521 agggccagac caagaccaag ctcgggaaca gcgaggtcaa gggcatcgtc gaggccatgg
     2581 tgaatgagtc cctgggctcc tatctcgagg agaatcccct ggtcgcccgc aaggtggcgg
     2641 aaaaggcatt gaatgcggcc cgtgcgcgcg aggcggccag gaaagcgcgg gagctgacgc
     2701 gcaggaaggg cgttctcgac tcgacgtcgc ttccgggaaa gctcgcggac tgccaggaga
     2761 aagacccggc cctctcggag cttttcatcg tcgagggcga ttccgcgggc ggctcggcca
     2821 aacagggccg ggacaggaag aaccaggcca tcctgccctt gaaaggcaag atcctgaacg
     2881 tggagaaggc gcgtttcgac aagatgctct cctccgatga gatcaaggtc ctgatcaccg
     2941 cattgggcgc cggcatcggg ccggaagagt tcgatatcgc caaggtccga taccataaaa
     3001 tcgtgatcat gaccgacgcc gatgtggacg ggtcgcacat tcggacactg cttctcacgt
     3061 tcttttatcg ccagatgctg cagataatcg agcgcggata cctctacatc gcccagccgc
     3121 cgctgttcaa gatcaaacgc ggcaagactg aacggtacat caaggacgat tcacgcatgg
     3181 aggatttcct cctggacctt gccgcggagg acatggagct cttctcgccc gagcagaacg
     3241 ggtatctcac gggagagcgc ctgacctcga cgctgaagaa gctcgtcgcc ttcgagaaaa
     3301 acctcgacaa gttcaagcga aagcggatcg acacggtggt gctccgggcg atcctcctgg
     3361 acaagacgtt caacaaggcc gtgctgaaag acggggagaa gctgaaaacc gtcgccgcct
     3421 ccatcgaggg ctatctcaag cactatcatc ccaagaccgt gatcgactgg gccatcgaaa
     3481 aggacgagga gcacgaggcc cataaactca ggttcgatac ccgatgggac gccatcgtct
     3541 cgagcatcgt attcgatgaa aactttgtca cctcgccgga attcagggag atccagagcc
     3601 tgtcgccggc catgctgggg cttgacgggc ccgattaccg catccgcgag aaaggcgagg
     3661 agaggacgtt ttcgcatacc ggcaagctcg tccattacgt gctcgagctc ggcaagaaag
     3721 gcctcgcgat ccagaggtac aagggcctgg gcgagatgaa ccccgagcag ctctggcaga
     3781 cgaccatgga cccggagaaa cggacgttcc tccaggtcac catcgaagac tcggtcaagg
     3841 ccgatgagat cttcacgatc ctcatgggcg acgccgtgga gccgcgccgc gcgttcatcc
     3901 agcggcatgc ccttgaagtg aagaacctgg acgtataaat gcagtgcgga atggcgaatg
     3961 cggagtgcgg aataaaaaac ccgcgaatcc gcagaaagaa aaagagctag atggaaccag
     4021 ttgaactgaa atcccagatc aacatcgagc aggagatgaa gaccgcctac ctcgactact
     4081 ccatgagcgt gatcgtgggg cgggcgctcc cggacgtgcg tgacgggctc aaacccgtgc
     4141 accgcaggat tctctacgcc atgttccgcg aggggctcct gcacaataag aaatattcca
     4201 agagcgcggg cgtggtcggg gaggtcctga agaagtacca tccccacggg gacagcgcgg
     4261 tttacgacac catggtcaga atggcgcagg acttcaacat gcgctacccg cttgtggacg
     4321 gccagggaaa cttcgggagc gtcgacggcg atccggcggc ggcctaccgg tataccgagg
     4381 cgaggcttac gaagctggcc gaggagatgc tgtccgacat cgacaaggag accgtcgatt
     4441 ttatcccgaa ttttgacgac acgattgttg agccgtcggt gctcccgacg cgcgtgccga
     4501 accttctcat caacggttcg gccggcattg ccgtgggcat ggctacgaac atacccccgc
     4561 acaatctttc cgaggtcgtg gacgggatcg tgatgatgat cgacaattcc gaggtctcca
     4621 tcccggagct tatgaccgcg atcaaggggc cggacttccc gacggccggc ttcatccacg
     4681 ggtatgaagg catcaagcag gcctatacca cgggccgggg gatcatccag atgcgggcac
     4741 gtgccatgat cgaggagggc aggggggagc gcgagagcat catcgtaacc gagctcccct
     4801 atcaggtcaa caaggccagg ctcctcgagc ggatcgccga actcgtgcag gacaagaaga
     4861 tcaccggcat tgccgagatc cgcgacgaat cggaccgcga aggcttgcgc atcgcgatcg
     4921 acctgaaaaa gggggagatc gccagcctca ccctgaacca gctctacaag cataccgcca
     4981 tgcagtccac cttcggggtc atcatgcttg ccttggtgaa caaccagccg cgggtcctga
     5041 acctccggaa actcctgtcg aacttcatcc agcaccgcag ggaagtggtg atcaggagga
     5101 cccggtacga cctcaggaaa gcggaagaga gggcccatat cctcctcggc ctcaagatcg
     5161 cccttgagaa tctcgacgcc gtgatcgccc tgatcaaggc gtcggccaat cccgacgagg
     5221 ccaagaccgg gctcgtccgc gagttcaaac tctcggagat acaggcccag gccatcctcg
     5281 acatgcggct ccagcgcctg accggcctcg agcgcgacaa gatcgtcgcc gagtacgagg
     5341 aggttctgaa agagatcgcc cgtttga
//
LOCUS       DASSLD010000437         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_234991,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000437 DASSLD010000000
VERSION     DASSLD010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234991"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1046
                     /locus_tag="VFK23_07920"
     CDS             72..1046
                     /locus_tag="VFK23_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET7319044.1"
                     /translation="MLPNFSYVRPKTIKDAVKQLSSEGSGVLAGGTDLLGCLRDGVFP
                     IKKAVSITDLADLRGIKKTADGGLAIGALTTVTEVAENKIIQETYPALAQAAAEVASP
                     QLRNQGTIGGNLCQKPRCWYYRGEFHCLRKGGKLCYAASGENQFHCIFGGGPCYIVHP
                     SDTAPALAAFNASVKTMGPKGTRTVAVDQFHVLPAVSVLRDTVLLPGEIMTGIILPPA
                     KGARSSYRKVRARNSWDFAVAGAAIAMSMEGGAVSKARVFLSGAAPVPWRSKAAEEAI
                     TGKRLGPDVIALAADVAVKGSQPLEHNGYKLAIFRGILQEELLKLAKA"
     gene            1204..>2119
                     /gene="pdhA"
                     /locus_tag="VFK23_07925"
     CDS             1204..>2119
                     /gene="pdhA"
                     /locus_tag="VFK23_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HET7319045.1"
                     /translation="MPEHVVESFAVKRLEILDAQGNIDQSLAPKLSDDEIKKMYELLV
                     LLRTFDQRLLALQREGRLGTYPSMLGQEASQVGSALAAEKSDWIFPTFREHGIYITKG
                     FPMHQLIQYWRGDERGLKIPEGLNVFPFAIPVGTQVPHALGAAMAAKYRKDRVAVLVY
                     FGDGGSSKGDVYEGMNMAGVFKAPVVFLCQNNQWAISVSREQQTASKTIAQKAYACGF
                     EGIQVDGNDIFAVYKATLDAANKAKEGGGPTLIECDTYRVADHTTADDATRYRQKEQI
                     EQWKARDPIARLRLFMEKKGLWSDAYQKD"
BASE COUNT          477 a          592 c          637 g          413 t
ORIGIN      
        1 ttcaaatttt ggatttgttt aggatttcga gattcgaact taggatttgc aattatgaag
       61 gaggtatccc catgctgccc aatttttcct acgtccgccc gaaaacgatc aaagacgccg
      121 taaagcagct ttcctccgaa ggcagcggcg tgctcgcagg cgggaccgac ctcctgggat
      181 gtctgcgcga cggcgttttt cccatcaaga aggcggtgag catcacggac ctcgcggacc
      241 ttcgcgggat caaaaagacc gctgacggcg gcctggcgat cggcgctctg actacggtca
      301 cggaggttgc ggagaataaa atcattcagg agacctatcc cgcgctcgcg caggccgccg
      361 cagaagtggc aagtccgcag ctccggaacc agggcaccat cggcggaaac ctctgccaga
      421 agccccggtg ctggtattac cgaggtgaat ttcactgcct tcggaagggg ggaaagctct
      481 gctacgcggc ctccggcgaa aaccagttcc actgcatctt cggcggcgga ccctgttaca
      541 tcgtccaccc gtcggacact gcgccggccc tggctgcatt caatgcgtca gtgaagacca
      601 tggggcccaa agggacaagg acggtagcgg tcgatcaatt ccacgttctt ccggccgtta
      661 gcgtgctgcg ggacacggtc ctcctgccgg gagagatcat gacggggatc atcctgcctc
      721 ctgcaaaagg cgcgcgaagt tcttaccgca aggtgagggc gagaaactcc tgggactttg
      781 ccgtggcagg agcggcgatc gcgatgagca tggaaggcgg cgcggtatca aaggcacgcg
      841 ttttcctctc cggtgcggct ccggtgccct ggagatcgaa ggccgcggag gaggcgataa
      901 ccggcaaacg acttggtccg gacgtgatag cgctggcagc ggacgtcgcg gtcaaaggat
      961 cacaacccct cgaacacaac ggttacaaac tggccatctt cagaggcatc ctccaggagg
     1021 aactgttgaa gctggcaaag gcttgattgc ccgtggttta aagcaaggcg ccccttttgg
     1081 gcgccttttt ttgtgtcgaa atcagtggtg ttgccacatt ccgcgttctc actgtcaggc
     1141 gcctctccga aactccctgc aggaagatgg tatacttaca gatattaagg aggggggaac
     1201 aggatgccgg aacatgtcgt ggagtccttc gcagtaaagc gccttgagat ccttgatgcc
     1261 cagggtaata tcgatcaaag cctcgcgcca aagctttccg atgatgaaat aaaaaaaatg
     1321 tatgagcttc tcgtccttct ccggaccttc gaccagcgcc tgctcgccct tcagcgggag
     1381 ggacgtctcg gcacgtaccc gtcgatgctg gggcaggagg cgtcgcaggt gggaagcgct
     1441 cttgcagcgg agaaatcaga ctggatattt ccgacctttc gggaacacgg aatctatata
     1501 accaagggct ttcccatgca ccagctcatt cagtactgga ggggagatga gcgggggctg
     1561 aagatcccag agggactgaa cgtcttcccg ttcgcaattc ccgtaggcac gcaggtcccc
     1621 cacgccctcg gagccgcgat ggccgcgaag taccggaagg accgcgtcgc cgttctcgtc
     1681 tatttcggcg acggcggcag ttcgaaaggc gatgtctacg aaggaatgaa tatggcgggc
     1741 gtgttcaagg ctcccgtcgt tttcctctgc cagaacaatc aatgggccat atcagtatcg
     1801 cgtgagcagc agaccgcgtc gaagaccatt gctcagaaag cctatgcttg cggtttcgaa
     1861 ggcatccagg ttgacggaaa cgacattttc gccgtataca aggccacgct ggacgccgcg
     1921 aacaaggcga aagagggtgg cggccccacg ctgatcgaat gcgacaccta ccgcgtggct
     1981 gaccatacga ctgccgatga cgctacccgt taccgccaga aggaacagat cgaacagtgg
     2041 aaggccagag acccgattgc acggcttcgg ctgttcatgg agaagaaggg cctctggagc
     2101 gatgcttatc aaaaggaca
//
LOCUS       DASSLD010000438         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_1740,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000438 DASSLD010000000
VERSION     DASSLD010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VFK23_07930"
     CDS             complement(<1..917)
                     /locus_tag="VFK23_07930"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HET7319046.1"
                     /translation="MKILFAASEAAPFAKTGGLADVAGSLPPALAELGHEVAVVMPRY
                     RQVDVASLRLKPVAYFFVPVGTWRERCDVLKGRMGRNVTAYFINKDEYFDRPELYGTP
                     RADYPDNAERFIFFSRAVLELCRALDFRPDIVHCNDWQTGLVPAYLKTIYHATPELRR
                     TKSVFTVHNLGYQGLFWHWDMKLTGLGWDVFTPEGLEFWGKISLLKAGLVYADSITTV
                     SRTYSREIQTSEYGSGLEGVLKKRSADLNGILNGIDYAEWNPGRRGDLPRPYDAAHLA
                     GKALCKKAVLGHLGVPDAGGPLIGMVTRLV"
     gene            complement(1084..2085)
                     /gene="galT"
                     /locus_tag="VFK23_07935"
     CDS             complement(1084..2085)
                     /gene="galT"
                     /locus_tag="VFK23_07935"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HET7319047.1"
                     /translation="MPELRKDIITGRWVIISSERGKRPNDFRDQRNQKRTGFCPFCAG
                     NERTTPREVLAYRDGGSAVPDGPGWRLRVVPNKFPVLQIEGDLNKAGEGIFDKMNGIG
                     AHEVIIESPDHNATLATLPRKAMEEVLWAYRDRMEDLKKDTRFQYALIFKNEGLTAGA
                     TLEHTHSQLIALPIVPIQVQEEIEGAKRHYNLKERCIFCDIIRQEMQTRTRVVLETPL
                     FIAIAPYAPRFPFETWIMPKKHLSCFACSSNDDFRDLAGLLQNVLTRIDRALSNPPYN
                     YVIHTSPFRDERNDYYHWHIELMPKLTNVAGFEWGSGFYINPTPPEDAAKFLREIKL"
BASE COUNT          445 a          679 c          705 g          488 t
ORIGIN      
        1 acgaggcgcg tgaccatgcc gatgagcggt cccccggcat ccggcacgcc gagatgcccg
       61 aggactgctt tcttgcagag ggccttgccg gcaagatgcg cggcatcgta gggccgcggc
      121 aggtcacccc tccgtccggg attccactcg gcatagtcga tcccgttcag gatgccgttg
      181 agatccgcgg aacgtttttt gagaacaccc tcgaggccgg acccgtactc agacgtctgg
      241 atctcgcggc tgtaggtccg gctcacggtc gtaatgctgt cggcatacac caggccagcc
      301 ttgagcaggc ttatcttgcc ccagaattcc aacccctcgg gcgtgaacac gtcccagccg
      361 agtcccgtaa gtttcatgtc ccagtgccag aacagtccct ggtagccgag attgtgcacc
      421 gtaaagaccg actttgtccg gcgaagctcc ggggtcgcat gatagattgt tttcagataa
      481 gcaggcacga gccccgtctg ccagtcgttg cagtgcacga tatcgggccg aaaatccagg
      541 gcacggcaga gctccaggac ggcgcgggaa aagaaaatga agcgttcggc gttgtccgga
      601 tagtccgccc ggggcgtacc gtagagctct ggcctgtcga agtattcgtc cttgttgatg
      661 aaataggcag tgacgttcct tcccatcctg cctttgagca cgtcgcaccg ttcccgccac
      721 gtccccacgg gaacaaaaaa ataagcaacc ggcttgagcc tcaacgatgc gacgtccacc
      781 tgccggtagc gcggcatgac gacggcgacc tcgtggccca gctccgcgag ggcgggggga
      841 agacttcccg cgacgtccgc cagcccaccg gtcttcgcga agggcgccgc ctcagaggcg
      901 gcaaagagga ttttcatggt ttgcagtcaa gggtaattac tcaggtcagc gacgaatgga
      961 gacaagagca tgtaaaacca aaccgtcatt cccgaatgat tctctcggga atccattttg
     1021 aatttagccg tggcaaagcc aatggattcc cgctttcgcg gaaatgacaa cctgagtagt
     1081 ccgtcaaagt ttaatttccc gcaggaactt tgccgcatcc tccggcggag tgggattgat
     1141 ataaaatccc gatccccatt cgaaaccggc gacgttcgtc agcttgggca tgagctcgat
     1201 gtgccagtga tagtagtcgt tgcgttcatc ccggaacggc gaggtatgga ttacgtaatt
     1261 gtacggcggg ttcgagagcg cacgatcgat ccgggtgagg acgttctgca ggaggcccgc
     1321 caggtctctg aaatcgtcgt tggacgagca ggcgaagcac gaaaggtgct tcttgggcat
     1381 gatccacgtc tcgaagggga agcgcggcgc ataaggcgcg atggctatga agaggggcgt
     1441 ctcgaggacg acgcgcgtcc tggtctgcat ttcctgccgg atgatgtcgc agaagatgca
     1501 tcgctctttg aggttgtaat gccgcttggc gccctcgatt tcctcctgca cctggatggg
     1561 cacgatcggc agggcgatga gctgcgaatg cgtatgctcc agggtcgcgc cggcggtgag
     1621 cccctcgttc ttgaagatca gggcgtactg gaaacgggtg tctttcttta ggtcctccat
     1681 gcggtcccgg taggcccaga gcacctcttc catcgccttt ctcgggagcg tagcaagcgt
     1741 ggcgttgtgg tccgggcttt cgatgatcac ctcgtgcgcg ccgataccgt tcatcttgtc
     1801 gaagatgccc tcgcccgcct tgttgaggtc gccctcaatc tggaggacgg ggaacttgtt
     1861 cgggaccacc cgaagccgcc agcccgggcc gtcggggacg gctgagccgc cgtcacgata
     1921 tgccaggacc tctcgcggcg tggtccgctc gttgccggcg cagaaggggc agaaccccgt
     1981 ccgtttctgg ttgcgctggt ccctgaaatc gttggggcgt tttccccgct ccgatgatat
     2041 gatgacccac cggccggtga tgatgtcttt tcgcagttct ggcatgattc actcctcggt
     2101 ccccgatcgt tgcatctaag gcgaagtctt ttcattataa tccatcccgt tcccggttgt
     2161 caaaagaact cttgctgtgc tatgatggga cgatatggcg cgcggcatcg acaaactctc
     2221 gacaaccctt gccaggatac tcaaggcccg cgggctggag ggccggcttc acgagtaccg
     2281 catcttcgca tcgtgggtcc ggacggtggg gcctgcc
//
LOCUS       DASSLD010000439         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1339127, whole genome shotgun sequence.
ACCESSION   DASSLD010000439 DASSLD010000000
VERSION     DASSLD010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339127"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="VFK23_07940"
     CDS             <1..1461
                     /locus_tag="VFK23_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET7319048.1"
                     /translation="FIVIIYGIVQKYGYDGLVIATILAGILLVVMGVAKLGAVIKYIP
                     YPVTVGFTAGIALIIAMGQVRDLLGLRMEKVPAEFVDKVEEYARHAATITPAAAFLGV
                     LTILIVALWPRVTKRVPGSLVAIIATTAMVPLFNLPVETIGSRFGAVSSTPPLPHLPH
                     IDWSKVPHMISPAISIALLAGIESLLSAVVADGMTGRRHRSNMELVAQGAANIASPFF
                     GGIPATGAIARTATNIKNGALTPVAGLVHAVVLLLIMMFFGKWAALIPMATLAGILMI
                     VAYNMSEYHLFIKMFTSPKSDIAVLIVTFLLTVLVDLTVAIQAGVVLAAILFMKRMAD
                     VTQVGYITSMMEDEEDTSDDDPNSIKRYTVPKGVEVFEVNGPFFFGAADRFKHVLSLI
                     VRKPKVLILRMRTVLSMDATALRALEEVYAQTQREGSRLLLAGVHAQPLFAMDRAGFL
                     DKIGVDNVFENVNDALKRARDLIGTSQEKWQEATVQ"
     gene            1915..>2140
                     /locus_tag="VFK23_07945"
     CDS             1915..>2140
                     /locus_tag="VFK23_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319049.1"
                     /translation="MGKERINFYSAFLYLLVGIVSTITSLYLTVKYFLISGNSWYILD
                     ISIDFLMLLALFGTFVILLQKTSETVKILLF"
BASE COUNT          453 a          635 c          576 g          476 t
ORIGIN      
        1 ttcatcgtga tcatctacgg catcgtgcag aagtacggct acgacggcct cgtgatcgcg
       61 acgatcctgg ccggcatcct gctcgtggtc atgggtgtcg caaagctggg ggccgtcatc
      121 aaatatatac cctatccggt cacggtaggg ttcaccgccg gcatcgccct catcatcgcc
      181 atggggcagg tgcgcgacct gctgggactc cgcatggaga aggtcccggc cgagttcgtg
      241 gacaaggtcg aggaatatgc gagacacgcc gccacgatca ccccggctgc ggcattcctg
      301 ggggtgttga ccatcctgat cgtcgctctc tggccccgcg tgaccaaacg ggtcccgggc
      361 tcgctcgtcg ccatcatcgc cacgactgcg atggtgcccc tgttcaacct gccggttgag
      421 acgatcggga gccgcttcgg tgcggtctcg agcacgccac ccctgccgca ccttccgcat
      481 atcgactgga gcaaagtgcc gcacatgatc tccccggcca tctccatcgc cctcctggcg
      541 ggcatcgaat ctctcctctc cgccgtggtc gcggacggta tgaccggccg gcgtcaccgc
      601 tcgaacatgg agctcgtggc ccagggggcc gcgaacatcg cctcgccgtt tttcggcggc
      661 atcccggcaa cgggcgcgat cgcccggacc gcgacaaata ttaaaaacgg cgcgctcacg
      721 ccggtggcgg gcctggtcca cgcggtcgtg ctgctcctta tcatgatgtt cttcggcaag
      781 tgggccgcgc tcatccccat ggccacgctc gcgggcatcc tgatgatcgt ggcctacaac
      841 atgagcgagt accatctgtt catcaagatg ttcacaagcc cgaagagcga catcgccgtg
      901 ctgatcgtga ccttccttct gaccgtgctc gtggacctca ccgtggcgat ccaggcgggc
      961 gtggtccttg ccgcgatcct gttcatgaag cgcatggccg acgtgacgca ggtgggatac
     1021 atcacctcga tgatggagga cgaggaagac acttcggacg acgacccgaa cagcatcaag
     1081 cgctataccg taccgaaggg cgtcgaggtc ttcgaggtga acggcccgtt cttcttcggc
     1141 gccgccgacc ggttcaagca cgtgctctca ctcatcgtcc ggaaacccaa ggtgctcatc
     1201 ctgcgcatgc gcacggtcct gtccatggac gccacggccc tccgcgcgct cgaggaagtc
     1261 tatgcacaga cgcagcgcga ggggagcagg cttcttctcg ccggcgtgca tgcccagcct
     1321 ctcttcgcca tggaccgggc cggcttcctc gataagatcg gcgtggacaa cgtgttcgag
     1381 aacgtcaacg acgccttgaa gcgcgcgcgt gacctgatcg gcacatcgca ggagaagtgg
     1441 caggaggcga ccgtgcagta agtcaactgg gacaagaaga agcccccgga aacagccctg
     1501 tgatctcaat accggcaagc cggaatacgg ggcgtctcgc gcaaagacgc caaggacgca
     1561 aaggaaacca taaagcagaa tctctttttt gttttaaacc aacagcattc ttaaagattt
     1621 ccttcgcgtc cttcgcgcct tcgcggtaaa aggttttgat ttggtttcta aaaacattcc
     1681 tcctgttctg tgtaattgta tccatctccc atcccccgac aatacccatc tccgggtatt
     1741 gacatcgttt tgaatcaaga atatagtcat ttccgggcac agcaaaactt atacgtatac
     1801 tttatataat tttagttgcc gaggcctaac ggcttaactg ctgacggctt gaatggacca
     1861 cgtgccaacc ggcagctcga gcctgcaagg ctcaaccttc caaggaagta agtaatgggg
     1921 aaagagagaa ttaactttta ttcagcattt ctttatttgt tggtgggaat agtatcaaca
     1981 attacatctc tctatcttac agttaaatat tttttgatat cgggaaacag ctggtacatt
     2041 ctcgatatat ctatcgattt cctgatgcta ttggcattgt ttggaacttt tgtaatctta
     2101 ttacagaaaa cctctgaaac agtaaagata ttgctttttc
//
LOCUS       DASSLD010000440         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1339518, whole genome shotgun sequence.
ACCESSION   DASSLD010000440 DASSLD010000000
VERSION     DASSLD010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339518"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /gene="yidC"
                     /locus_tag="VFK23_07950"
     CDS             <1..1228
                     /gene="yidC"
                     /locus_tag="VFK23_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET7319050.1"
                     /translation="ALSKLSQVEYRADHDAVRLGKEKSAETLVLTYAGPAGISIEKKL
                     TFYNNDYKVDIAVTTRGLDGYALSLGTDFGLADKVSKDASGRVGLVALADGKTITEKL
                     ESIKGEVQHAGAVEWFSQADKYFTATILYGSNGIVTARRTAAPKEIGDLLTTDLTVKE
                     KPEARTFTLYAGPKSFTLLQAHGHGLEQMVDYGWFGILAKPMFWLLRQFFTITHNYGV
                     AIILLTIVVRILLFYPSLKSATAMEEMKKIQPQLMAIREKYKKDPARVNQEMMKLYKE
                     HKVNPLGGCLPMLLQLPFFVALYNVLSVSIELRQAPFISFWIKDLSVYDPYYILPVLM
                     GVSMIFTMKMTSTTADPQQAKIMMFMNIAFIFLFAWLPAGLLLYITLSNVLSIVQQLY
                     VRRILGVKDGSAAVSA"
     gene            complement(1344..>2443)
                     /locus_tag="VFK23_07955"
     CDS             complement(1344..>2443)
                     /locus_tag="VFK23_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET7319051.1"
                     /translation="LHSIDMIINSSLDLGVTLQEFLNFVISQLKVDAAAVLLLNPHTQ
                     TLEFAAMLGFRNPDIRDSRLRLGEGVAGRAALERRTIRIPDLNAPENDFLRSSLIEGE
                     GFVSYYAIPLISKGQVKGVLDILHRSALKVDEEWMNFLASLAAQAAIAIDNAALFTDL
                     QRSNADLILAYDTTIEGWARALELRDKETEGHTKRAVEMTLRLARAMGMDEAALVHVR
                     RGALLHDIGKMSVPDEILLKEGPLSKEEWEIMRRHPVYAFEMLYPISYLRPALDIPYC
                     HHERWDGSGYPRGLKGEQIPLAARIFSLADIWDALNSNRRYHAAWPEEQVREHIRSLA
                     GTQFDPRVVEMFLRMYPAQREEVGAVNPRTS"
BASE COUNT          505 a          734 c          717 g          487 t
ORIGIN      
        1 ggccctctcc aagctctcgc aggtggagta ccgcgcggac catgatgcag tgcggctcgg
       61 caaagaaaaa tccgccgaga cccttgtcct tacctatgcg ggtccggcag gcatctcgat
      121 cgaaaagaaa ctgacttttt acaataacga ttacaaggtc gatatcgccg ttacgacgag
      181 gggcctggac ggctacgccc tgtcgctggg gacggatttc ggcctcgccg acaaggtgag
      241 caaagacgcc agcggccgcg ttggcctggt tgccctcgcg gacggaaaaa caatcaccga
      301 aaagctcgag agcatcaagg gagaagtgca gcacgccggc gccgtagaat ggttcagcca
      361 ggcagacaag tacttcacgg cgacgatcct gtacggatcg aacggcatcg tcacggcgag
      421 aaggaccgcc gcgccgaagg agatcggcga ccttttgacc acggacctca cggtgaagga
      481 aaagcccgag gcccgcacct tcaccctcta cgccggcccg aaaagcttca cgctgttgca
      541 ggcccacggc cacggcctgg aacaaatggt cgactatgga tggttcggca ttctcgcgaa
      601 gccgatgttc tggcttctgc ggcagttttt cacgatcacg cataattacg gcgtcgcgat
      661 cattcttttg acgatcgtgg tgaggatact cctgttctac ccctccctca aaagcgccac
      721 ggccatggag gaaatgaaaa agatccagcc gcagctcatg gcgatccgcg agaaatacaa
      781 gaaagacccg gcccgcgtca accaggagat gatgaagctc tacaaggagc acaaggtcaa
      841 ccccctgggc gggtgcctgc ccatgctcct gcagctgccg ttcttcgtcg cgctctacaa
      901 cgtgctgtcc gtgtccatcg agctcaggca ggcccccttc atctccttct ggatcaagga
      961 tctgtccgtg tatgatccct actatatcct tcccgtgctg atgggcgtct ccatgatctt
     1021 taccatgaag atgacctcga cgacggcgga cccgcagcag gccaagatca tgatgttcat
     1081 gaacatcgcc tttatttttc tctttgcctg gctcccggcg ggcctcctgc tctatatcac
     1141 gctcagtaac gtcctctcga tcgtccagca gctttacgtg cgcaggatcc tcggcgtcaa
     1201 agacggttcc gcggccgtct ccgcatagca aacccctctt ctgccagctc ccggcaccgt
     1261 cgcgggagcg ggcgcttcat gctcatcggg attcgattgc cgttttgtaa aaaggaattt
     1321 gattcgtctc ggtgcggacc ggtttatgac gtccggggat tgacggcgcc gacctcttcg
     1381 cgctgcgccg gatacatgcg aagaaacatc tcgacgaccc tgggatcgaa ctgcgtgccg
     1441 gcgagagaac ggatatgttc acggacctgt tcttccggcc acgcggcatg atatcgccga
     1501 ttggagttta gcgcgtccca gatgtcggca agggaaaata tgcgcgccgc gagagggatc
     1561 tgttcgcctt tcaacccgcg tgggtagccc gagccgtccc accgctcatg atggcagtag
     1621 gggatatcga gcgcaggccg gagataggaa atgggataca gcatttcgaa ggcgtagacc
     1681 ggatggcgcc gcatgatttc ccactcttcc ttcgacagag ggccttcctt gagcaggatt
     1741 tcgtccggaa cgctcatctt gccgatgtcg tgcaggagcg cccccctgcg cacgtgaacg
     1801 agcgcggcct cgtccatgcc catggcgcgg gcgaggcgca gcgtcatctc cacggccctc
     1861 ttggtatggc cttccgtctc cttgtccctg agttccagcg cccgcgccca tccttcgatc
     1921 gtggtgtcat aggcgaggat gaggtcggca ttggaccgct gcaggtcggt gaacagcgcg
     1981 gcattgtcga tggcgatggc tgcctgcgca gccagggacg caagaaaatt catccattcc
     2041 tcatcgactt tgagcgccga tcggtggaga atgtccagca cgcccttcac ctgccctttc
     2101 gagatgagcg gaatggcata gtaggaaacg aacccctcgc cctcgataag ggatgagcga
     2161 agaaaatcgt tctccggcgc gttgaggtcg ggtatgcgga tcgtgcggcg ttccagggcc
     2221 gccctgccgg caacgccctc gcccagacgg agacgggaat cccggatgtc cggatttcgg
     2281 aagcccagca tcgccgcgaa ctcaagcgtc tgcgtgtggg ggttcaggag cagaaccgct
     2341 gcggcatcaa ccttgagttg agaaatgacg aagttgagga actcctgaag cgtcaccccc
     2401 agatccaggc tggagttgat gatcatgtcg atggagtgga gcg
//
LOCUS       DASSLD010000441         2728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_866542,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000441 DASSLD010000000
VERSION     DASSLD010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866542"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFK23_07960"
     CDS             complement(<1..186)
                     /locus_tag="VFK23_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319052.1"
                     /translation="MHLQGAFFKVSLLFALAVMVWACSSPNSKAPILDAAGKHAGNWI
                     VDHRPSYLRDQGPCRQCH"
     gene            complement(683..1060)
                     /locus_tag="VFK23_07965"
     CDS             complement(683..1060)
                     /locus_tag="VFK23_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7319053.1"
                     /translation="MPEYKFLVVEDSPTMRQLISFALKRIPGSKIVEANDGIDALKKL
                     STQKFDIILTDINMPIMDGLKLVSMVRNDPAHKAIPIVIITTEGAEEDRKRGLALGAN
                     AYIPKPIQTADLLNVVNGILGNK"
     gene            complement(1075..1902)
                     /locus_tag="VFK23_07970"
     CDS             complement(1075..1902)
                     /locus_tag="VFK23_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7319054.1"
                     /translation="MMEKEPPSISKKVEEFLQVFKKGEEFTQELLKENEKLRYKVAKL
                     EEVTKFSDREESFKFHTLEERVKFLEEENKQLLNRYRVVEEENKDFANRYIEVEAENN
                     NLANLYVASYQLHSTLDFNESLKIILEIVMNLIGAEEFSIMMLDEKTNELTIVAQEGM
                     GPEARPSVKLGEGVIGRAAKTGESFYREGDPTNLAGVDYLHPLVVIPLKIKEHVIGVI
                     VIYKLLVQKESFSNVDYELFSMLAGHAATALFSSKLYSQSERKLTTIQSFLDLLKEK"
     gene            2199..>2728
                     /locus_tag="VFK23_07975"
     CDS             2199..>2728
                     /locus_tag="VFK23_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319055.1"
                     /translation="MQTANDICSLDYLIGTLSTAYKGAITSLVAKDFCMTVAGEAIIL
                     RGRILSMPAGNYEASLIGRGVELARARLEDGSFEFKTGSGQVMHARDLQIDILQQGRH
                     IGTFLLKNEEAGGVYISAVELSEEIRGMDFKLLTIPLREKAGLLQKAEAIIAQIHSTK
                     KDWPAFSELLHGFAYDA"
BASE COUNT          553 a          770 c          769 g          636 t
ORIGIN      
        1 gtggcattgg cggcacgggc cctggtcgcg cagataggac gggcggtgat cgacgatcca
       61 gttcccggcg tgcttcccgg ctgcatcaag gatcggtgcc ttgctgttcg ggctgctgca
      121 cgcccatacc atgactgcca aagcgaaaag cagagaaact ttaaagaagg caccctgtaa
      181 gtgcataagt ttttctcctc cacatagtac cccccgcacc gcggcataat caactggaaa
      241 acttatcccc tttttcattc gcaatcaaag tgccccgctt cgtctcgtac cagcgactca
      301 tcatcgataa atctggttca gcatcggtcg cggacgaacc gtcgctccga atttactgca
      361 gcgacttcag atacatcgag agcgcctcga tatcatggtg acgtgtcggc gaggaagtca
      421 ttattccctc cttgcaagat tagttttttc tgcgattcat tcctgatgtg attctgcggg
      481 tttttcagcg aacgggagac acggacggag ggaaaaaccg ctcgtgaagt ttctcccgat
      541 acaataagcg tactataaca atgacccgat ttcaaggaag acggcgaaaa ggcatgggcg
      601 ccggcggcgg ggacgaacaa aaaagacggg aaaccgcatg gtctcccgtc ctttgctttg
      661 cctcagtctg tcccggcctt gtttatttgt tcccgaggat cccgttcacg acgttcaaga
      721 ggtccgccgt ctggatgggt ttggggatgt aggcgttggc gcccagggcg aggccgcgct
      781 tgcggtcctc ttccgcgccc tcggtcgtga tgatgacgat ggggatggcc ttgtgcgccg
      841 ggtcgttcct gaccatggaa acgagtttga gaccgtccat gatgggcatg ttgatgtccg
      901 tcaggatgat gtcgaatttc tgggtcgaga gcttcttgag cgcgtcgata ccgtcgttgg
      961 cctcgacgat cttgcttccc gggatgcgct tcagggcgaa ggagatgagc tgccgcatcg
     1021 tcggggagtc ttctaccacc agaaacttat actcgggcat gttccaacct ccgtttattt
     1081 ttctttgagc aggtcgagaa agctctggat cgtggtcagc ttccgctccg attgtgagta
     1141 gagtttcgag gaaaagagcg ccgtcgccgc atggccggcc agcatcgaaa aaagctcata
     1201 gtcgacgttc gaaaaagatt ctttctggac gaggagcttg taaatgacga tgacaccgat
     1261 cacgtgctcc ttgatcttga gcggaatgac gacgagggga tggagatagt ccacgcccgc
     1321 gaggttcgtc gggtcgcctt cgcggtagaa ggactcgccg gtcttcgccg ccctgccgat
     1381 gaccccctcc cccagcttga cgctgggccg ggcttcgggc cccatgccct cctgggccac
     1441 gatcgtcagc tcgttggtct tctcgtcgag catcatgata gagaactcct cggcgccgat
     1501 caggttcata acgatctcga gaatgatctt gagcgactcg ttgaagtcca gcgtggagtg
     1561 aagctggtag cttgcgacgt acaggttcgc caggttgttg ttctcggcct cgacctcgat
     1621 gtagcggttc gcgaagtcct tgttttcctc ctccacgacg cggtagcggt tgaggagctg
     1681 cttgttctcc tcctccagaa atttcacgcg ctcttcgagg gtgtgaaatt tgaagctttc
     1741 ttcccggtcg gagaacttgg tgacctcctc gagcttggct accttgtatc ggagcttctc
     1801 gttttccttg agcagctcct gggtgaactc ctcgcctttt ttgaaaacct gcagaaactc
     1861 ttcgactttt ttcgaaatcg acgggggttc tttttccatc attcctcctg tagtcacttc
     1921 cgtctgattc agcgacccgg gacgccggtg aggagcgtat aactcggagc attatagtct
     1981 agactcctga aattatcaag cgggaattct gaagaattct ggagatacct atacgaacct
     2041 tcacttcgcg aagcgcgcga atacgataat gcttctcacc ctgagaatcg agctcccgtt
     2101 ccccagggaa gctcaattct cctccgcagc cgcgccttcc cgggcccgga agggcctgca
     2161 atcccgcttc cccgcgtgct agacttaaag caggcgctat gcagaccgcg aacgacatat
     2221 gcagtctcga ttatctgatc ggcacgctct ccacggcata caagggcgcc atcacctcgc
     2281 tcgtggccaa ggatttctgc atgaccgttg ccggggaagc cataattctg agggggcgga
     2341 tcctttccat gcctgcggga aattacgagg cgtcgctcat cggccgcggc gtcgagcttg
     2401 cccgggccag gctcgaggac gggtccttcg agttcaagac ggggagcggg caggtcatgc
     2461 acgcgcgaga cctccagatc gacatcctcc agcagggcag gcacatcggg accttcctgc
     2521 tcaagaatga agaggccggc ggcgtctata tctcggccgt ggagctgtcc gaggaaatca
     2581 ggggcatgga tttcaagctc ctcaccattc cgctgcggga gaaggccggg cttctccaga
     2641 aggccgaagc aatcatcgca caaatccact cgacgaaaaa ggactggccg gcattctccg
     2701 agctgttaca cggctttgcc tatgacgc
//
LOCUS       DASSLD010000442         3262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_783586,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000442 DASSLD010000000
VERSION     DASSLD010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783586"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..488)
                     /locus_tag="VFK23_07980"
     CDS             complement(150..488)
                     /locus_tag="VFK23_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319056.1"
                     /translation="MTRPSAVAIVPAVRAAAVAAAFTVPVAPAMLVLGFIFYLLLAFG
                     GPAAKFRFSSSVISCFSRSPVVTRLHLRRMFRSNLCYLRGLGFLGFFRPCLCKRASAA
                     NDKAGEKCDG"
     gene            977..1915
                     /locus_tag="VFK23_07985"
     CDS             977..1915
                     /locus_tag="VFK23_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319057.1"
                     /translation="MLVGEARAEFTLDLEVREAYEDNVIGLVADNPNITSTAATAAAG
                     GAAGEVAAGDKRGGGSGGGGGGRALHMRKGGGELNDIPTTVTTTPTTTTTTTTAAAGA
                     AGGIVTAGDFSTSFAVDAGYLHDLGDITTLVFLASVDHTGYMRYTQFNFTIVSLRAGV
                     SVALTDDLTGKVLVGESVKRFNNSNRNANAMGASFSLKERFTPAFWVKGVYSYEHNNA
                     DSFLDSYTGNSYSIWAGYNLTSKTAVGVGYSYLTRDFKSSQFRLISKTASADFTWDFY
                     GNWSMSVGFDHERGDSNAPNSATIDNIYSVGLLYSY"
     gene            complement(2000..>3262)
                     /locus_tag="VFK23_07990"
     CDS             complement(2000..>3262)
                     /locus_tag="VFK23_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HET7319058.1"
                     /translation="GNVIPPVGAALCDKGAFEFRPQKIVVTPASLAFGIVTIGAPANQ
                     TITVSNAGDNPLVIGTIGGLNPLAAPFSFLVNNCVGALATLTPIPLPAQTCTVTVDFS
                     PTAATASSDSFDIPSNDPAALSVAVAVAGTGSAVPVPGISVTDSIAPSDDHLLAFGNV
                     TIGLTSDATVTVTNTGTADLVIGTIAAPAAPFSITSDGCSGTTLAASAACALTVHFAP
                     TANSVANATFDIPHNITGTPPVTMTLTGSGVSATGNHAPSIPVLVSPANGQTGVPTTM
                     TFVWNKSVDPDSDTVTYHFLNCTDTGFTTGCTNNDVTAAAPFSLYFAGIGSFGAGIIL
                     IGFVAKNGTRRARRMMLVFALLLVSGTFFVACHKSRSEPPPPAPAEQISNIVRNLTPN
                     TSYYWKVVADDGKGGTSTSAVSSYTTGQ"
BASE COUNT          706 a          891 c         1016 g          649 t
ORIGIN      
        1 aaagcatgcc gcgcggccag taaacccgcc gttttcgtca tgccggactt gatccggcat
       61 ccaggggttt tgaaggactg gattcccgct ttcgcgggaa tgacattctc atagtagcaa
      121 aggaggaata agccatgata cgagcactct tatccatcgc atttttcgcc ggctttgtca
      181 ttggcggcgg aagcgcgttt gcacaggcaa ggccggaaaa agcccagaaa cccgaggccc
      241 cgcagatagc acaagttgga acggaacatc cggcggaggt ggagccgggt gacgacaggg
      301 gagcgggaga agcaggagat gacagaggac gagaagcgga acttcgcggc agggccgccg
      361 aaggcgagca ggagatagaa aatgaagccg agcaccagca tcgcgggcgc gacagggacc
      421 gtgaacgcgg cagcgaccgc agcggcccgg accgcgggca cgatggcgac ggcggagggc
      481 ctggtcatga cggaggaggc cgtggaggac gcaattaact cccggctata aaacagtaac
      541 gaatgcgtaa aaaaaccgtg agaggatgta tctctcacgg tttttttatt gctccaaatt
      601 cgggtaagga ccattaaatg acctttgagt tccgatgagc ggccgcgttg tgatatactg
      661 agtttgtaat tactcagtat tacgaatgtt tcaaaccaac ccgtcattcc cgaacgtaag
      721 accatccccc ctcccttttt ccccccttga aggggagggg caggggtggg gatgcgtttg
      781 tcttccggga atccagaggt tttggtttta taccgagaaa gaccctggaa cccgccccag
      841 catgattgaa gcaggggcgt gaatgacgac ctgagcagat gtctcaattt taattctctt
      901 cacgatccgg ggaggcgaca tgattctgtc cgcaaaaagc cgtttcattg ctatcatttc
      961 aatatccgct ctatttatgt tggttggaga ggcacgcgcg gaattcacgc tcgatctgga
     1021 agtgcgcgag gcatatgagg acaatgtcat cggcctggta gccgacaacc ccaatatcac
     1081 ctcgaccgcg gcaacagctg ccgcgggcgg ggccgcgggt gaggtcgcgg ctggagataa
     1141 acgcggaggc gggagcggcg ggggcggcgg gggccgcgcg ctgcatatga gaaaaggcgg
     1201 cggcgagttg aatgatatac caacgaccgt gaccactact ccgacgacca cgacgaccac
     1261 gacgaccgct gctgccgggg cggccggagg gatagtaacc gccggtgatt tctcgaccag
     1321 cttcgccgtg gatgccggct acttacatga tctcggagac atcacgaccc ttgtgtttct
     1381 ggcctcggtg gaccataccg gatacatgag gtacacccag ttcaacttta ccatcgtttc
     1441 gctgcgggcc ggcgtctcgg tcgccctgac cgatgacctt accggcaagg tgctggtcgg
     1501 agagtccgtg aaaaggttta ataacagcaa tcgcaacgcc aatgccatgg gcgcatcgtt
     1561 cagcctgaag gagcggttca ctccggcgtt ctgggtgaag ggcgtctatt cctacgaaca
     1621 taacaatgcc gattcattcc tcgattccta cacggggaat tcgtacagca tctgggccgg
     1681 atacaacctg acatcgaaga ccgcggtagg ggtgggttac agctacctta cgcgggactt
     1741 caaatcatcc cagttcaggt tgatttccaa gaccgcttcc gccgatttca cctgggactt
     1801 ctacggaaac tggagcatga gcgtcggatt cgaccatgaa cgcggagact ccaatgcccc
     1861 caactccgcc acgatcgata atatttacag cgtgggattg ttgtactcct attgatcgcc
     1921 gcgcaatgct gaatgaatcc aaaaagaaag ggcagccgca tcgtgtgcgg ttgcccttct
     1981 tgtttttaca gggacgcggc tactgacccg ttgtatagct cgaaaccgcg ctcgtcgaag
     2041 ttcctccctt gccgtcgtcc gctaccacct tccagtaata gctcgtgttc ggcgtgaggt
     2101 tcctcacgat attgcttatc tgctcggcgg gtgcgggagg cgggggctca cttctgctct
     2161 tatggcaggc cacgaagaag gtccccgaga ccagcagaag ggcgaagacg agcatcatcc
     2221 tccgcgcgcg tctggtgccg tttttcgcga caaagccgat caggatgatg cccgcgccga
     2281 agctgccgat gccggcaaag tacaggctga agggagcggc tgcggtaaca tcgttgttcg
     2341 tgcatcccgt cgtaaagccg gtatcggtgc agttcaggaa atggtacgtt accgtgtcgc
     2401 tgtcgggatc aaccgatttg ttccagacga aggtcatcgt cgtgggtacg cccgtctgcc
     2461 cgttcgccgg agaaacgagc acagggatgg agggagcgtg attgccggtg gccgataccc
     2521 cggaccccgt caaggtcatg gtcaccggcg gcgtgccggt tatgttgtgc ggaatatcga
     2581 aggtcgcgtt ggccacgctg tttgccgttg gggcaaaatg aaccgtgagc gcgcaggcgg
     2641 cagacgctgc cagcgtcgta cccgagcagc cgtcgctggt gatgctgaaa ggagccgcag
     2701 gcgcggcgat cgtcccgatt accagatctg ctgttcccgt attggtgacg gtgaccgtcg
     2761 catcggacgt cagaccgatc gtcacgttgc cgaaggccag caggtgatcg tcactcggag
     2821 ctatagaatc ggtgaccgag atgcccggga ccggcaccgc ggaccccgtg ccggcgactg
     2881 cgacggcgac ggacaaagcc gcggggtcat tactcgggat atcgaaggaa tcggacgatg
     2941 cggtggctgc agtcggcgaa aagtccacgg tcaccgtgca ggtctgcgcg ggcaagggaa
     3001 tcggcgtcag tgtcgcaagg gcacccacgc agttgttgac caggaaactg aacggcgccg
     3061 caagcgggtt cagtccgccg atggtgccga tcaccagcgg gttgtcgccg gcgttgctta
     3121 ccgttatcgt ctggttggct ggtgctccga tcgtcactat gccgaacgcc aggcttgcag
     3181 gagttaccac gattttttga ggtctgaatt caaaggctcc cttgtcgcag agtgccgcgc
     3241 cgacgggcgg aataacattg cc
//
LOCUS       DASSLD010000443         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1081139, whole genome shotgun sequence.
ACCESSION   DASSLD010000443 DASSLD010000000
VERSION     DASSLD010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081139"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..373)
                     /locus_tag="VFK23_07995"
     CDS             complement(14..373)
                     /locus_tag="VFK23_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7319059.1"
                     /translation="MGEKLKVYQMNLEGEIDAAKMAEINRVIGQLIKGEMYGLIMNFE
                     SVEHIHFTILPSLMEEKRRLQSFGGDIRLAAMSDYLKNIFRTTGVLEEFQVFDTVQQA
                     ARSFGVARPATNLPLGL"
     gene            complement(378..812)
                     /gene="mraZ"
                     /locus_tag="VFK23_08000"
     CDS             complement(378..812)
                     /gene="mraZ"
                     /locus_tag="VFK23_08000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HET7319060.1"
                     /translation="MFRGSYEHTVDSKGRVSVPSKFRDIIADRYDGRLVLAMDYDRCL
                     TVYPLEEWERLEEKIKTLSLMKQEVKDFRRFLLSSATECELDKQGRILIPPAHREHAA
                     ISKNVMVVGIIEKIEIWDVKTWEARNIQNGDKIGEALAALGL"
     gene            995..1312
                     /locus_tag="VFK23_08005"
     CDS             995..1312
                     /locus_tag="VFK23_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007280916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET7319061.1"
                     /translation="MENDFFISADDEHIKREKAKAREMRGSQWWKRKRSAGRCYYCGG
                     TFKPSELTMDHLVPIVRGGKSVQGNLVPACKQCNSKKKYLLPSEWDEYLESLKKERKG
                     EKD"
     gene            complement(1523..2104)
                     /locus_tag="VFK23_08010"
     CDS             complement(1523..2104)
                     /locus_tag="VFK23_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HET7319062.1"
                     /translation="MNGEQISRYPVPELQELPQDIKDRIQNVQEKAGFVPNVFLALAH
                     RPEEFRAFFAYHDALMEKEGGLTRAEREMIVVATSGINQCPYCVVAHGAILRVRAKNA
                     LIADQVAVNYRKADITPRQKAMLEFAVKVSRHAEEIGDADFDALRQEGFSDEDIWDIG
                     AISAFFALSNRMANMIDLRPNDEFYLIGRLPKQ"
BASE COUNT          500 a          649 c          629 g          541 t
ORIGIN      
        1 attccgcatt tcttcacagc cccagcggaa ggttcgtcgc cggcctggca acgccgaaac
       61 tcctggccgc ctgctgcacc gtgtcgaata cctggaactc ctcaagcacg ccagtcgtcc
      121 ggaagatgtt cttgaggtaa tccgacatcg ccgcgagccg gatgtcacca ccgaaagatt
      181 gcaggcgcct cttctcttcc atcagggagg gaaggatcgt gaagtggatg tgctccacgg
      241 actcaaagtt catgatcagt ccgtacatct ctcccttgat gagctgaccg atcacgcggt
      301 tgatctccgc catcttggcc gcgtcgatct caccttcaag gttcatctga tatactttga
      361 gtttttctcc catgtcgtta aagacccagc gcggcgagcg cctcgccgat cttgtcgccg
      421 ttctgaatat ttcgcgcctc ccacgtcttg acgtcccaga tttctatttt ctcgatgata
      481 cccacgacca tcacgttctt cgagatcgcc gcgtgttcgc ggtgggccgg agggatcagg
      541 atgcggccct gcttgtcaag ttcgcattcc gttgccgacg agaggaggaa ccgtctgaag
      601 tccttgacct cctgcttcat cagggagagc gtcttgatct tctcctcgag ccgctcccat
      661 tcctcgagcg ggtagaccgt gaggcagcgg tcgtagtcca tggcgagcac gagccgtccg
      721 tcgtaacggt cggcgatgat gtcgcggaac ttcgatggta cgctaactct ccccttggaa
      781 tctacggtat gttcgtagct gcccctgaac atggatctcc cttatgccac cgtctcccac
      841 ttcataccac cttctgggca aagtatatat ttcatggata gtaatgtcaa gaaaaatatt
      901 aattttttca gatgttacgc ggtgggtaag gcgtgggcaa gaggcgcctt ttttagcgtg
      961 acaaggggcc ggggaaaaag taatataggg gccgatggaa aacgattttt ttatcagcgc
     1021 cgacgatgag cacatcaaac gcgaaaaggc aaaggcccgc gagatgcgcg ggtcgcagtg
     1081 gtggaagcgg aagcgttcgg caggacgctg ctattattgc ggcgggacgt tcaagccttc
     1141 cgagctcacc atggaccact tggtcccgat cgttcgcgga ggaaaatccg tgcaggggaa
     1201 cctcgttccc gcgtgcaagc aatgcaacag caaaaaaaaa tatctcctcc cctcggaatg
     1261 ggatgagtat ctcgaatcgt tgaagaagga gcggaaggga gagaaggact aggggccaaa
     1321 tcaactcagg ccgtcattcc cgcgaaagcg ggaatccatt ggtttcttca aagctaaatt
     1381 caaaacttgt cctcgaatgg tttaatcggg gagggattcc cgatagaacc attcgggaat
     1441 gatggtttgg ttttacgcgg tctttgcggg ttttgcgaac tttgcggaaa aatttcccgg
     1501 ctgagaagaa gttgaattat tgttactgct tcggcagccg tccgataaga tagaactcgt
     1561 cgttcggccg caggtcaatc atgtttgcca tgcggttcga cagggcgaaa aaagcgctga
     1621 tggcgccgat gtcccagatg tcctcgtccg aaaatccctc ctgcctcagc gcgtcgaagt
     1681 cggcgtcgcc gatctcctcg gcatgacggg acaccttcac ggcgaattca agcatcgcct
     1741 tctggcgcgg agtaatgtcg gccttgcggt agttcacggc cacctggtcg gcgatcaggg
     1801 cgttcttcgc gcgtacgcgc agaatggcgc cgtgggccac gacgcagtac gggcactggt
     1861 tgatgcccga ggttgcaacc acgatcatct cgcgttccgc tctcgtgagc cctccctcct
     1921 tctccatgag cgcgtcgtgg tacgcgaaga acgcgcgaaa ctcctccggc ctgtgggcga
     1981 gcgcgagaaa cacgttcggc acgaagccgg ctttttcctg cacattttga atccggtcct
     2041 tgatgtcctg cggcagttcc tgaagctccg gcacgggata ccggctgatc tgctctccgt
     2101 tcatcggcga cctccttccc ggcattattg ttttagcata tcacatccgc cgccgataga
     2161 cggaacactt ccgtctccat gaacacaatc ctactcctga cacgcgtgaa ccagaaaaga
     2221 gtcatccccg catgaccctg tcggggatgt ggattaaacc atattcccca ttaacaggat
     2281 gttgaaaaag tcgaaatatt ctggaattgt cactctgag
//
LOCUS       DASSLD010000444         5939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1504251, whole genome shotgun sequence.
ACCESSION   DASSLD010000444 DASSLD010000000
VERSION     DASSLD010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504251"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VFK23_08015"
     CDS             complement(<1..1194)
                     /locus_tag="VFK23_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HET7319063.1"
                     /translation="MKRFVQLRKPYYLFLAALFIMALTTVPAHGAGRAPEKGVSQESA
                     NFLKKLSVALDEVADIARPAVVNISTTSTVTMQENPFGDMFNDPFFRRFFGDQFEHPG
                     QKRQYKSSALGSGVIVSEQGYILTNNHVVKGAEEIKVILYDKREFKGKVVGSDPRTDI
                     AVVKIDAKDLPTVKFGASSKLKTGDIVLAIGNPFGLNQTITMGIVSAVGRSNIGLADF
                     EDFIQTDAAINPGNSGGALVNTDGELVGINTAIFSTSGGYMGIGFAIPADMVKSVMDS
                     IIKYGKVIRGWLGVTIQNLTPDLSKSLNIKETAGALISGVEKDSPADKAGLKRGDLIV
                     ELNGKKVDDSTMLRNMVSQSAPGTKADFKIMREGKEKTVTATLGEYKEKQVVKKAEYD
                     NVLRGV"
     gene            complement(1372..4698)
                     /locus_tag="VFK23_08020"
     CDS             complement(1372..4698)
                     /locus_tag="VFK23_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET7319064.1"
                     /translation="MNDAIDTSKSIHLSASAGSGKTRALKDRYLALLDVLDHAGLNID
                     QAVAITFTDKAAAEIRERVMQGLPEPMLKKIIRGREDLRISTIHSFCMNLLKRYPIEA
                     GLPPDFGVLDERDRVYKVRQAVEDALEESGGDPQIMIPFGAAAADDLIAAVEFLLSIR
                     SRLKRLEIDAGGPEGLLHNIRAGMEIDGQEAELRTLMKGTQWRARLTRMERVLRSLQD
                     RYDDNQGNAHLLLAETGNADTALRITESLAPLYFTAAGEPRKNPSIAKKQFTGKNRAD
                     YENAWFSVQELLARFRDVAGRVRAGKKALALLRLYLLAGEKYEESKLREGLLDFDDLE
                     IRAYGLLQGGESPDILYWLDRKVLHFLVDEFQDTSDIQWAVLSRLTEEIFAGQGADKR
                     MSPTLFVVGDEKQSIYRFREANYRLLGNVRLKMESNIPVESRKILTLDKNFRSAPEII
                     ETVNQVFGSLWGESYKPSEAERKGHRGSVRLIEILPGYDETKTGPTEADVLAREIRSI
                     VESGIMVYEKSADSPLFRNDGAIKGDIWQQRRVGYGDCAVLIQSRTRLKEYEASLQKE
                     SVPFRVVGGIGFYEEGEIQALIHTLFFLWNRDDLHSLASALKSDLFGFTDKDLYDLTA
                     GGGNMLDALKERRPDVAGLLRGWSGLAGLAPLSTLIHQIVKDTGAYVRFGRRNPQAIF
                     NIDKLLDTAREFDGRGYITLQDFVEWVKNIREAEQREATADMNLPGFQGAVSIMTVHK
                     AKGLEFPVCFLPGMNQKPQSATSGPPVIVEGDGRVRLAVRNGKSPLYDEMWDREREEL
                     QREHQRLLYVAMTRARDHLIMTGTLRDEKKPLVQNTWLEYLHSAAPKPLFEAKVEAQP
                     GMRVYSYPAAGEIPAPVHLTTRRTEETAPRGQEGLETEGGKKLDIQQILLNLSPVPLS
                     KSLEWKRATDFIEHERETELELLSLRTAAGAVSPLTRGSVFHRCLEEYVKQGSYDLSL
                     IIAEYPEIRILASEAVRAFRDDAESVLKAVTTDKELSWIFARGNGSYAELPFLYRRGN
                     ALVSGIVDRVLVRGDKGFVIDYKAILIENDEALAIWNSHYRPQLQIYCEAAKDIFGLR
                     SVEGSLLFLDSRRLERVVSL"
     gene            complement(4695..>5939)
                     /locus_tag="VFK23_08025"
     CDS             complement(4695..>5939)
                     /locus_tag="VFK23_08025"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET7319065.1"
                     /translation="SAAHHFYRLLLSALRVTLTYPENVGDRPVVASPFLQELTPLRQA
                     GLLNQGIGKTAAIQFSLRIDESHSLPELAKAVSLAGDTPGLTDLLNADIEGMSGIRTA
                     ARQRAAAAVPKFPVAPHGKREFSVTELDLYLKCPYDYLVARVLDVAPLEEVTEDISPL
                     ERGSKVHTVLRNFYLSWTGPVSRERRDEARALLKRLADPEFDNEADTFRNRREKELFL
                     TVMAERFLDAEEEFWGQGMRPAYLEQKMERYPLVLRDGGEIGLSAKIDRIDVDEDGNF
                     IIVDYKTGDYPRPRMNTEQDIFQLPVYAVMAGSALKDGDPALKRCIGLAYYDLSGRSG
                     AGARDVVLYDKDARGGHPSSKPKASAKSADEFRAILNQSMDKARMAVEGILAGDFYSA
                     PKDDSRCRSCANEAMCDRGNEE"
BASE COUNT         1066 a         1778 c         1768 g         1327 t
ORIGIN      
        1 aacgccccgg aggacattgt catactccgc tttttttaca acctgtttct ccttgtattc
       61 tcccagtgtg gccgtcacgg tcttctcttt gccctctctc atgatcttga aatccgcctt
      121 cgtgccgggc gccgactggg agaccatgtt ccggagcatc gtcgaatcgt cgactttctt
      181 gccgttcaac tcgacgatga ggtcaccccg cttgagccct gctttgtccg ccgggctgtc
      241 tttctcgacg cctgatatca gtgctccggc ggtctccttg atgttcagcg atttcgacag
      301 atcgggcgta agattctgga tggtcacgcc cagccagccg cgtatcacct tgccgtactt
      361 gatgatgctg tccatgaccg acttcaccat gtcggctggt atggcaaacc cgatccccat
      421 gtatcccccg ctggttgaaa agatcgcggt gttgatgccg acaagttcac catcggtgtt
      481 taccagggca ccgcccgagt tgccgggatt gatggccgcg tcggtctgga tgaagtcctc
      541 aaaatcagca agaccgatgt tcgagcgtcc tacggcgctc acgataccca tggtgatggt
      601 ctggttcagg ccgaaggggt tgccgatggc cagtacgatg tccccggtct tcagcttgct
      661 cgatgcaccg aatttaaccg tcggcaggtc ctttgcgtcg atcttgacca cggcgatgtc
      721 cgttctcggg tcggagccga cgaccttgcc cttgaactcc ctcttgtcgt acagaatcac
      781 cttgatctct tcggcaccct tgaccacgtg gttgttcgtg aggatatacc cttgttcgga
      841 cacgatgaca ccggagccca gggcggacga tttatattga cgcttctgtc cggggtgctc
      901 gaactgatct ccgaagaacc ggcggaagaa agggtcattg aacatgtcgc cgaagggatt
      961 ttcctgcatg gtgaccgtcg aggtcgtcga gatgttcacg accgcgggcc gcgcgatatc
     1021 cgccacctcg tcaagcgcca cgctcagttt cttaagaaag tttgccgact cctgggagac
     1081 gcctttttcc ggtgcgcggc ctgcgccatg ggcgggcacg gtcgttaatg ccatgatgaa
     1141 cagggctgca agaaacaggt aatagggttt tctgagttgc acaaagcgtt tcataagtcc
     1201 tccttcatga tgatatttca tggaacagat tgcttatcct tgtatcactt cacggacggg
     1261 tcaaatttat aatacaaaag aatcaaaaat gcaagtcgca aggaatcagt atgagcatat
     1321 cggatatgag tatactacga attcaggaat cgtgcagaca ggtttggcgg actagaggct
     1381 gacgacccgc tccagcctcc tgctgtccag gaaaagaagt gagccttcca cgctcctcag
     1441 tccgaagata tcctttgcag cctcgcaata tatctggagc tgcggccggt agtgcgaatt
     1501 ccatatcgca agcgcctcat cgttctcgat gaggatggcc ttgtaatcga tgacgaaccc
     1561 cttgtcgccc ctgacgagca cccggtcgac gattccactc acgagggcgt tcccccgtct
     1621 gtacaggaac ggcagttccg cgtaggagcc atttccccgc gcaaaaatcc aagaaagttc
     1681 tttgtccgtt gtgaccgcct taaggacgga ttcagcgtca tcgcggaacg cccggacggc
     1741 ctcagatgcg aggatgcgga tttcgggata ttccgcgatg atcaggctga gatcatacga
     1801 tccctgtttt acgtactcct cgagacagcg gtggaacacg ctcccgcggg tgaggggaga
     1861 gacagcgccc gccgcggtcc ggagcgacag cagttcgagc tctgtttccc gctcgtgctc
     1921 gataaagtcg gttgcccgct tccattccag agactttgac agcgggaccg gggaaaggtt
     1981 gagcaggatt tgctgaatat ctagtttctt tccgccttcc gtctcaagtc cctcctggcc
     2041 ccgtggcgct gtctcttccg tacgccttgt tgtgaggtgg acaggagcag ggatttcccc
     2101 ggcggcggga taggagtaca cgcgcatccc cggctgcgcc tcaaccttcg cttcgaacag
     2161 cggtttcggc gccgcgctgt gcaggtattc aagccacgta ttctgaacaa gcggtttctt
     2221 ctcgtctctc agcgtccccg tcatgatcag atggtcccgg gcgcgcgtca tggccacgta
     2281 caggaggcgc tgatgctcgc gctggagttc ttcccgttcc cggtcccaca tctcgtcgta
     2341 cagcggactc ttcccgttcc tgacggcaag gcggaccctc ccgtcgcctt caacgatcac
     2401 gggggggccg ctcgtcgcgg actggggctt ctggttcatg ccgggcagga agcagacggg
     2461 gaactcgagt cccttggcct tgtgcacggt catgatgctg acggcgccct ggaagccggg
     2521 caggttcata tccgccgtcg cctcgcgctg ttccgcctcc ctgatgtttt taacccactc
     2581 gacaaagtcc tggagtgtga tgtaaccccg gccgtcgaac tcccgggccg tatcgagcag
     2641 cttgtcgata ttgaagatcg cctgcggatt gcgccttccg aaacggacgt aggccccggt
     2701 gtcctttacg atctgatgga taagggtgga aaggggagcg aggcccgcga ggcccgacca
     2761 gccgcgcaag agcccggcca catccggtct gcgctccttc agggcgtcga gcatgttccc
     2821 gccgcccgcc gtgaggtcgt agaggtcctt gtccgtgaag ccgaaaaggt ccgatttgag
     2881 cgccgaggca agcgagtgca ggtcatcgcg gttccagagg aagaagaggg tatggatcag
     2941 ggcctggatc tcgccctctt cgtaaaagcc gatgccgccg accacgcgga aggggacact
     3001 ttctttctgg agggatgcct cgtactcctt gagcctggtc cgggactgaa tgaggacggc
     3061 gcagtcgccg tatcccacgc ggcgctgttg ccagatatca cccttgatcg cgccgtcatt
     3121 cctgaagagg ggggaatcgg ccgacttttc gtaaaccata attccactct cgacaatcga
     3181 tcttatctcc cgggctagga cgtccgcttc cgtcgggccg gttttcgttt catcataacc
     3241 gggcaaaatt tcgatgagcc ttacgctccc ccggtgtccc tttcgttccg cttcagaggg
     3301 tttgtacgat tccccccaga gagagccgaa tacctggttc acggtttcga tgatctcggg
     3361 cgcggaccgg aaattcttat cgagcgtcag gatcttcctc gattccacgg ggatgttgct
     3421 ctccatcttc aggcgcacgt tacccagcag tcggtagttc gcctcgcgga agcggtagat
     3481 ggactgtttc tcgtcaccca caacgaagag ggtgggggac atgcgtttgt ccgcaccctg
     3541 gcccgcgaag atctcctcgg tcagcctgct caggacggcc cactggatgt cgctcgtgtc
     3601 ctggaactca tccacgagaa agtgaaggac cttccggtcg agccagtaga ggatgtccgg
     3661 cgattcaccg ccctggagca ggccgtaggc acggatctcg aggtcgtcga agtccagcag
     3721 gccttcccgg agcttggact cttcatattt ttcgccggcc agcagataga gccgtagaag
     3781 cgccagggcc ttttttcctg cacgtacgcg gccggcaaca tcgcgaaaac gggccagcag
     3841 ctcctggacg gagaaccagg cattctcgta gtcagcccgg ttcttgccgg tgaactgttt
     3901 cttcgcgatc gaggggtttt ttctcggttc tccggcagcg gtgaaataga gcggggcgag
     3961 ggattccgtt atccggaggg cggtgtccgc gttcccggtc tccgcaagaa gcagatgggc
     4021 gtttccctga ttgtcatcat accggtcttg cagggaccgg aggacccgtt ccatccgcgt
     4081 aaggcgcgcg cgccactgcg tgcctttcat cagcgttctc agttccgctt cctgcccgtc
     4141 gatttccatg ccggcgcgga tgttatgcag cagtccctcc ggcccgcccg catctatttc
     4201 caggcgtttg agcctgctcc ttatcgacag caggaattcc acggccgcga taaggtcgtc
     4261 cgcggcagcg gcgccgaacg gaatcatgat ttgcggatcg ccgccggact cttccagggc
     4321 gtcttcaact gcctgccgga ccttgtaaac acggtcgcgc tcgtcgagca cgccgaaatc
     4381 cggcggaagc cccgcctcga tgggatagcg cttcaggagg ttcatgcaga aggagtggat
     4441 cgtggaaatg cggaggtctt cccttccccg aatgatcttt ttgagcatgg gttcgggaag
     4501 cccctgcatc acccgctccc tgatctctgc cgcggccttg tccgtgaagg tgatggctac
     4561 agcctggtct atgttgagcc ccgcgtggtc gaggacgtcg aggagcgcga ggtatcggtc
     4621 cttgagagcg cgggtcttgc cggagccggc ggaggccgag aggtggatag acttcgaggt
     4681 gtctatggcg tcgttcattc ttcgttcccc cggtcgcaca tcgcctcatt cgcgcaggag
     4741 cggcacctgc tatcgtcctt cggcgcggag taaaagtcgc ccgccaggat gccttccacg
     4801 gccatgcgcg ccttgtccat gctctgattc aggatcgccc ggaattcatc ggcgcttttc
     4861 gcgctcgcct tgggcttcga tgagggatgg ccgccgcgcg cgtccttatc atagaggacg
     4921 acgtcccgcg ccccggctcc ggaccttccc gagaggtcat aataggcaag gccgatgcac
     4981 ctcttgaggg cgggatcgcc gtccttgagc gcggacccgg ccataacggc gtagacgggg
     5041 agctggaaga tgtcctgctc agtgttcatc ctgggcctgg gatagtcgcc cgtcttgtag
     5101 tccacgatga tgaagtttcc gtcttcatcg acgtcgatgc ggtcgatctt cgcggagagc
     5161 ccgatctcac cgccgtcgcg aaggacgagc gggtaccgct ccattttctg ttcgaggtat
     5221 gcgggccgca ttccctgtcc ccagaattcc tcttcagcgt caaggaaccg ctccgccatg
     5281 acggtgagaa aaagctcctt ctcgcgccgg ttgcggaagg tgtccgcctc gttatcgaac
     5341 tccgggtcag caagtcgctt gaggagcgcg cgggcctcgt ctcgtctttc ccgggagacg
     5401 ggaccggtcc atgaaaggta gaagtttctg aggacggtgt gcaccttgct gccgcgctcc
     5461 aggggcgaga tgtcctcagt tacttcctcg agaggcgcca cgtccaggac cctcgcgacc
     5521 aggtagtcat aggggcattt caggtagagg tccagttccg tgacggaaaa ctcgcgcttc
     5581 ccgtgcggcg cgaccggaaa cttcggaacc gctgccgctg cccgctgccg ggcggccgtc
     5641 cgtatgccgg acatgccctc gatatcggcg ttcaggaggt ccgtcaggcc gggcgtgtct
     5701 cccgcgaggc tgaccgcctt tgccagttcg gggaggctat ggctttcatc gatccggagg
     5761 ctgaactgaa tggccgctgt ttttccgatc ccctggttga ggagcccggc ctgtctgagc
     5821 ggcgtaagct cctgcaggaa gggggaggcg accaccggcc ggtcgcctac gttttcgggg
     5881 tacgtgaggg ttactctcag cgcagagagc aggagccggt aaaagtgatg ggccgcgct
//
LOCUS       DASSLD010000445         3692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1155279, whole genome shotgun sequence.
ACCESSION   DASSLD010000445 DASSLD010000000
VERSION     DASSLD010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155279"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VFK23_08030"
     CDS             <1..141
                     /locus_tag="VFK23_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014954357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HET7319066.1"
                     /translation="VGDVDFEAVSKKTSLITPVPGGVGPVTIAILLKNTVISAKRMVG
                     LG"
     gene            complement(272..1462)
                     /locus_tag="VFK23_08035"
     CDS             complement(272..1462)
                     /locus_tag="VFK23_08035"
                     /inference="COORDINATES: protein motif:HMM:NF027862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein T N-terminal
                     domain-containing protein"
                     /protein_id="HET7319067.1"
                     /translation="MIRSLKVLVLSLCIMAVTVVSIRAQETRTVTAEGVAAIQGNAKD
                     IARDAAIEDAQKRAVEQAIGILIDSQTQVENYQLISDKILSQTKGYITRYNVSGESVD
                     SGLLRVSINAEVALGKLTDDLSGIGILLGQMHKPRTMIMIAEQNIGHEWYAWWWGYGN
                     HREHEQTEMGVVENTFMDAFTKKGFEFIDHAAAAKEIKVTPAYKVQDLNAQQAQTLGN
                     QADAEVVIVGKALAKLYGDIGGGMKSVQADLSVKAIRTDTGQVIAAATTHAAAVHITP
                     ETAGIDALKKASQSAADQMMEKILAVYSKEVGGTKPVNITISGLDKTQFVKFKDVLRN
                     QVRGIKDVHERSFSGGVAKLSVDSKSSAQMLSDELSLKDFGTFSVEIMGSTANSLELK
                     VTPK"
     gene            1665..2381
                     /locus_tag="VFK23_08040"
     CDS             1665..2381
                     /locus_tag="VFK23_08040"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HET7319068.1"
                     /translation="MPELSVNIDHIATIRQARRGSEPDPVAAAALAELAGARGIIAHL
                     REDRRHVQDRDLRLLRDVVQTMLNMEMAATDEMQRIAREIKPDFSTLVPEKREELTTE
                     GGLEVASRADFMKSYIGRLQEAGITVSLFVDPDEKQIAAARKAGADWVELHTGSYANA
                     LAGEDREREFVKLSDAAKLAASLGLRVGAGHGLNYVNVVRIAGIPEVEELNIGHSIIS
                     RAALVGMDRAVRDMIGLFQS"
     gene            2505..2879
                     /locus_tag="VFK23_08045"
     CDS             2505..2879
                     /locus_tag="VFK23_08045"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET7319069.1"
                     /translation="MIVGIGVDLVKTSRIDRAGRDHPGFLERVFTEQEREYCARQKFP
                     AQHYAGRFAAKEAVLKAIGTGWSAGVKWTDMEVLHGEGGGPIVNISGRVKDLMDLKGV
                     KQVHLSYSHDEGYAVAQVVLVG"
     gene            2972..>3692
                     /locus_tag="VFK23_08050"
     CDS             2972..>3692
                     /locus_tag="VFK23_08050"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HET7319070.1"
                     /translation="MKNIDRRAIRGFGIPGPVLMENAASAILTEMERFFDGLDGMRVG
                     ILCGKGNNGGDGLALARRLRIRGVPVRVALLAPFAEVAGEAKVNLSILRRTDVEIMEN
                     AAARSLSDVISWSDVLVDAMLGVGLASPLRGAYARAVSLVNQSGKPVVAVDVPTGIDS
                     DTGAVLGAAVRADLTVTMALMKRGLALYPGAEFAGTVRVADIGIPPEVIEREKIELSL
                     LDRGSAWGVLHRRRRDSHKGSY"
BASE COUNT          708 a         1068 c         1175 g          741 t
ORIGIN      
        1 gtgggcgacg ttgatttcga agcggtctcg aagaagactt ccctcatcac gccggtgccg
       61 ggcggggtcg ggccggtcac catagcgatc ctgctcaaga acacggtgat ctcggcaaag
      121 agaatggtgg ggctgggatg acttcagttc ggagtaggga gttgggagtt cggagaacaa
      181 agacttcagt tcggagtcgg gagttcggag ttctgagagt ttttgttggt tcaacagtca
      241 ctccgaaccc cgaactaaga actccgaact gtcatttcgg cgttaccttc agttcgagcg
      301 aattggctgt cgaacccatg atttccacgc taaaagtccc gaagtccttg agcgaaagct
      361 cgtcggaaag catctgggcg ctcgacttgg agtccacgga aagcttcgcc acaccgccag
      421 agaaactccg ctcatgcacg tctttgatgc cgcgcacctg gttccggagc acgtccttga
      481 atttgacgaa ctgggtcttg tccaggccgg agatcgtgat gttgaccggc ttcgtgcctc
      541 cgacttcctt cgaataaacc gccagtatct tttccatcat ctggtccgcg gcggactgcg
      601 acgccttctt gagcgcgtcg atcccggccg tttccggagt tatgtgcacg gccgccgcgt
      661 gagtggtcgc cgccgcgatg acctgccccg tatcggtgcg gatggccttg actgagaggt
      721 ccgcctggac ggacttcatg ccgccgccga tgtccccgta gagcttggcc aacgccttac
      781 ccacgatcac gacttcggcg tccgcctggt tgcccagggt ctgcgcctgc tgggcgttca
      841 ggtcctggac cttgtaggcg ggggtcacct tgatctcctt agccgcggcc gcatgatcga
      901 tgaattcgaa cccttttttc gtgaaggcgt ccatgaaggt gttttcgacg acgcccattt
      961 ccgtctgctc gtgttcacga tggtttccgt atccccacca ccacgcatac cactcatggc
     1021 cgatgttctg ctcggcgatc atgatcatgg tgcggggctt gtgcatctgg cccagcagga
     1081 tgccgatgcc cgagaggtcg tccgtcagct ttccgagagc gacctcggcg ttgatgctga
     1141 cgcgcaggag accgctgtcc acggactcgc ccgacacgtt gtaccgtgtg atataaccct
     1201 tggtctgcga aaggatcttg tcgctgatga gctggtagtt ttccacctgg gtctgcgagt
     1261 cgatgagaat gccgatcgcc tgctcgacgg cccgtttctg cgcgtcctcg atcgccgcgt
     1321 cgcgcgcaat gtccttggca ttgccctgga tggcggcgac gccttcggcg gtaacagtcc
     1381 ttgtctcctg cgcccggatg gagaccaccg tgacggccat gatacagagc gagagaacga
     1441 gaactttcag ggagcgaatc atatatcacc tccggaaagg atgtctggac cgagacaacc
     1501 atattgcggt gattcacgcc atcgcggatg gctcggtcct tatgatagca aaggccgcgc
     1561 tcttggtcaa ccatgaaaaa tgagccgcca taaatcttct ggatattttg atggatagat
     1621 gataaaatct ttttcatcac gtttgcgcca ggagggattg ccgcgtgccg gagttgtccg
     1681 tcaacatcga tcatatcgcc acgatacgcc aggcaaggag gggttccgag ccggaccccg
     1741 ttgccgcagc cgcgctcgcc gaacttgccg gggcccgagg catcatcgcc catctccgcg
     1801 aagaccgcag gcacgtgcag gaccgcgacc tgcgcctcct gcgcgacgtc gtgcagacca
     1861 tgctgaacat ggaaatggcg gcgaccgatg agatgcagcg gatcgcccgc gagatcaagc
     1921 ccgatttttc cacactggtg ccggaaaaac gggaagagct cacgaccgag ggcggactgg
     1981 aggtcgcgtc aagggccgat ttcatgaagt cctacatcgg ccgcctccaa gaggcaggca
     2041 taaccgtgag cctcttcgtc gaccccgatg agaagcagat cgccgcggcc aggaaggcgg
     2101 gcgccgactg ggtcgaactc cacaccggtt cttacgcgaa tgcgctcgcc ggggaggacc
     2161 gcgaacggga attcgtcaaa ctctcggatg ccgcgaagct cgccgcgagc ctggggttgc
     2221 gcgtgggcgc gggccacggc ctgaactacg tgaacgtcgt acggatcgcg ggcatccccg
     2281 aagtggaaga gctgaacatc gggcacagca tcatctcgcg cgccgcgctc gtggggatgg
     2341 accgggcggt cagggacatg atcggattgt ttcaatcgta aaagcgaaat tgaccgcgga
     2401 ggcgcggaga tcgccgagga agtcctttcc attcgtgttt tttttctccg cgtctcggcg
     2461 tctctgcggt ggtcatgcgg aatttgtttt cctgaggggg gagtatgatc gtcggcattg
     2521 gcgtagacct ggtgaagacc agccgcatcg acagggcggg cagggaccat cccggttttc
     2581 tcgaacgcgt gttcaccgag caggagcggg aatactgcgc caggcagaag tttcccgccc
     2641 agcactatgc cggcaggttc gccgcaaagg aagcggtgct caaggccatc ggtacgggat
     2701 ggagcgccgg tgtcaaatgg acggacatgg aagtgcttca cggagagggc ggggggccga
     2761 tcgtgaacat ctcaggaagg gtgaaggacc tcatggacct gaaaggcgta aaacaggtcc
     2821 atctgagcta ctcccacgat gaagggtatg ccgtggcgca agtggttttg gtagggtaag
     2881 agtcaggagt caggagtcag gagtcaggag tcaggagtca ggagtcagga gtagtacctt
     2941 aactgctatg aaaattgtca cagcacagca gatgaagaac atcgatcgca gggcgatccg
     3001 gggcttcggc atccccgggc cggtccttat ggagaacgct gcttccgcga tcctgacgga
     3061 gatggaaagg ttcttcgacg gtctcgatgg tatgcgggtc ggcatcctct gcggcaaggg
     3121 caataacgga ggcgacggcc tcgcgctcgc ccgcaggctc cgcatccgcg gcgtgccggt
     3181 ccgtgtcgct cttctggccc cttttgccga ggttgccggc gaggcgaagg tcaacctgtc
     3241 catcctccgc aggacggacg ttgaaatcat ggaaaacgcc gcggcccgct ccctctccga
     3301 cgttatctcg tggtccgacg tcctggttga tgcaatgctc ggcgtgggcc tcgcttcccc
     3361 gctccggggc gcctatgcca gggccgtctc cctggtgaac cagtcgggca agcccgtggt
     3421 tgccgtcgac gtcccgacag gcatcgactc cgacaccggc gctgtcctgg gagctgcagt
     3481 cagggctgat ctgacggtca cgatggccct catgaagcgc gggctggcac tgtatcccgg
     3541 ggccgagttc gccgggacgg tccgcgtcgc ggacatcggg atccctccgg aggtcatcga
     3601 gcgggaaaag atagaactga gcctcctcga ccgcgggagc gcctggggcg ttctgcaccg
     3661 gcggaggcgg gacagtcaca agggaagtta cg
//
LOCUS       DASSLD010000446         2415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_16466,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000446 DASSLD010000000
VERSION     DASSLD010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16466"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFK23_08055"
     CDS             <1..331
                     /locus_tag="VFK23_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HET7319071.1"
                     /translation="GKGKGGRNQELALAFAQEIAGTEGITLLSAGTDGTDGPTDAAGA
                     VVDGETATIAAKIEIHPEVYLERNDSYAFFAKLDRMIDDKHHIITGPTGTNVMDLQVI
                     LVEGKAV"
     gene            328..1605
                     /locus_tag="VFK23_08060"
     CDS             328..1605
                     /locus_tag="VFK23_08060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7319072.1"
                     /translation="MRLASGTGTIASRKELCKELECIVSADILVGIPSFNNARTIGHV
                     VRAVQAGLAKYFPDRKAVLIDSDGGSTDGTADIVQNTVIENFKSILLHHRVEPAFKMS
                     IPYHGLPGKGSAFRAIFEIAEALQVKACAVVDSDLRSITPEWVELLIKPVLESEVDYV
                     APLYRRHKYDGTITNSIIHPLTRALYGKRVRQPIGGDFGFSGKLARHYLSKDVWNTDV
                     ARYGIDIWMTTEAIANNFKVAEAFLGAKIHDPKDPGADLSAMLYQVVGSAFGLMESYD
                     KVWKTTRGSEAPASFGFQYEVGLEQVPVDAGRMLRMFRAGVKSLKEIWVAVLGQGDFS
                     ALESLSECPDEEFRFPLSLWARVVYDYALAYHMRKLPHEHLLRSLTPLYLGKTASFVM
                     EVRDRNQSEAEAEIEKLCLEFEAGKDYLVNNWK"
     gene            1621..2319
                     /locus_tag="VFK23_08065"
     CDS             1621..2319
                     /locus_tag="VFK23_08065"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319073.1"
                     /translation="MEAFDSVIFDQLMESYYRFARFVPNLLAMLIIILVGIGIVRIVR
                     VILLKVLHAIKFDTWSDRMGLTSFMRKGDLWHKSSEAVSSFVYWFMIIGVLMVGISSL
                     NIQAMDSLITRFVMYGPRLLSATLIIVIGYIATGFISRAVLISTVNRGFLFARLIAET
                     VRVLLTVLFIAMTLDELEVAPRIVVTAFAILFGGIILGLSISFGVGGIDAARKIIERE
                     TEKKEEEKQDIEHI"
BASE COUNT          545 a          653 c          728 g          489 t
ORIGIN      
        1 cggaaaagga aagggaggga gaaaccagga actggcgttg gccttcgccc aggaaatcgc
       61 agggacagag ggaatcaccc tgctttcggc cgggacggac gggacggacg gcccgactga
      121 cgccgcgggc gcggtggtag acggtgagac ggcgacgatt gccgcaaaaa tcgagattca
      181 tcctgaggta tatttagaga ggaacgattc gtacgctttt ttcgcaaagc tcgaccgaat
      241 gatcgatgac aagcaccaca tcataacggg gccgacgggt acgaacgtga tggaccttca
      301 ggtcatactc gtcgagggaa aggctgtatg aggttagctt ccggaacagg cactatagca
      361 agcagaaaag agctctgcaa agagcttgaa tgcatcgtat ccgccgatat cctcgtcggc
      421 atcccgagtt tcaacaacgc ccgcaccatc ggccatgtcg tgcgggccgt gcaggccggc
      481 cttgcaaaat atttccccga ccggaaggcg gtgctcatcg attccgacgg cgggtctacc
      541 gacgggactg ccgacatcgt gcagaacacg gtcattgaga atttcaaatc catcctgctc
      601 caccatcggg tcgaaccggc cttcaagatg tccatcccct atcacgggtt gccgggcaag
      661 gggagcgcat tccgggcgat cttcgagatc gccgaggcgc tccaggtaaa agcctgcgcg
      721 gtcgtcgact ccgacctccg gagcatcacg cccgagtggg tcgaactgct gataaagccg
      781 gtgctcgagt cggaagtcga ttatgtcgcg ccgctgtacc gccggcacaa gtacgacggg
      841 acgatcacga acagcatcat ccaccccctg acccgggcgt tgtacggcaa gcgggtcagg
      901 cagccgatcg ggggggactt cggtttttcc gggaagctcg ccaggcatta tctgtccaag
      961 gacgtctgga atacggacgt cgcacggtac ggcatcgaca tctggatgac gacggaagcg
     1021 atcgcgaata atttcaaggt ggcggaagca ttcctcggcg ccaagatcca cgatcccaag
     1081 gacccgggag cggacctctc cgccatgctg tatcaggtcg tgggctccgc cttcggtctc
     1141 atggagagct atgacaaagt atggaagacc acacgcggtt ccgaggcgcc ggccagcttc
     1201 ggattccaat atgaggtcgg tctggagcag gtgccggtgg acgcaggcag aatgctccgg
     1261 atgttcaggg ccggggtaaa gagcctgaaa gagatatggg tcgccgttct cggacaggga
     1321 gatttctccg cgctcgagtc cctgagcgaa tgtcccgatg aggaattcag gtttcctctc
     1381 agcctgtggg cccgcgtcgt ctacgattac gcccttgcat accacatgcg gaaactgcct
     1441 cacgaacatc tgctccggtc cctgacgccc ctctacctgg ggaagactgc ttccttcgtc
     1501 atggaggtgc gggacaggaa ccagagcgaa gccgaggcag agatagaaaa gctctgcctg
     1561 gagttcgagg cgggcaagga ttacctggtc aataactgga agtaatccgg gaggtgcgct
     1621 atggaggcat tcgactctgt aatctttgat cagttgatgg aatcgtatta ccggttcgcg
     1681 aggttcgttc cgaatctcct ggccatgctc atcatcattc tggtcggcat cggcatagtg
     1741 cggatcgtgc gtgtgatcct gcttaaagta cttcatgcaa tcaagttcga cacctggagc
     1801 gacaggatgg gcctgacctc gttcatgaga aaaggggacc tctggcacaa gtcgtccgag
     1861 gccgtgtcat cgttcgtgta ctggttcatg attatcgggg tattgatggt gggaatcagc
     1921 tccctgaaca tacaggccat ggacagcctg attacgcgtt tcgtcatgta cggaccgaga
     1981 ttgctgtctg cgaccctgat catcgtcatc gggtatatcg cgacgggctt catcagcagg
     2041 gccgtcctca tctcgacagt gaaccgggga ttcctcttcg cgaggttgat tgccgagacc
     2101 gtgcgggtcc tcctgaccgt gctgttcatc gcgatgaccc tggacgaact cgaggtggcg
     2161 ccccgcatcg tcgtaaccgc gtttgcaatc ctcttcggag gcatcattct gggcctgtcc
     2221 atatccttcg gcgtaggcgg tatcgatgcc gcgcgaaaga tcatcgagag agagacggaa
     2281 aaaaaggaag aggaaaaaca ggacatagaa catatatagc gtgtatatta ctgtcatacc
     2341 tgcgaaagca ggaatccatc ttgaatccgg tctcgaaaaa gccaatggat acccgctttc
     2401 gcgggtatga caggg
//
LOCUS       DASSLD010000447         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1437978, whole genome shotgun sequence.
ACCESSION   DASSLD010000447 DASSLD010000000
VERSION     DASSLD010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437978"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VFK23_08070"
     CDS             <1..488
                     /locus_tag="VFK23_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET7319074.1"
                     /translation="RYLGLGLETITASLKGEGVPAGAFLLKILFTAITLSFGGSGGII
                     TPIFFIGATAGSAFGRALGFDPALFADMGMVGLLAGAANTPISASIMAIELFGPAIAP
                     YASVACVISFLMTGHRSVYPSQVLSLAKSSSLTVAKGSEMRDAGTWNITPVRKASAAV
                     S"
     gene            628..1671
                     /locus_tag="VFK23_08075"
     CDS             628..1671
                     /locus_tag="VFK23_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET7319075.1"
                     /translation="MDIPYKGIIAPAATAVGFTLALLIVRSVAFRILHAWAKRTETKL
                     DDIIIASTKNPSLLFALAIGVNIGLSFSGIPEKHLGYLGKILHVVIILSATIASASIV
                     GELFRNYVQKSNIPIPTTGLAYGILKGTIFVIGFLIILTDLGISIAPLITALGVGGLA
                     VALALQDTLANLFAGIHIMMEKSIRIGDFVKLETGQEGYVDDITWRTTRIRMLPNNMV
                     VIPNNKLAQSVVTNYYLPEKRMSLTIPVSVSYSSDPAKVERILVEEAKKGAASIPGLL
                     ADPEPFVRLIPGFGESSLDFTLICQVREFTDQYLAQHELRKSIFNRFREEGIEIPFPQ
                     RTVHLREEKEQKT"
     gene            1668..2333
                     /locus_tag="VFK23_08080"
     CDS             1668..2333
                     /locus_tag="VFK23_08080"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HET7319076.1"
                     /translation="MSEPFEFKQCVILIKATGKKAPGLRELRDLIAGASPASISHHTF
                     EYFLKGHTLEYTNDFAHWAGKSLEESALAEQLSNIDPYACRDVEELRSELLEAIDGHL
                     ERLPEPRAALPGDEFFFNETVAFIVPAGVRARNLAEFLIALKYVDESSLYYHFYNARQ
                     RLKSGRDEFSAWFATLGKEELAAAMKAIDPYMHTLQGIRERIAEAVEASLRRDIETAG
                     VGP"
     gene            2375..>2625
                     /locus_tag="VFK23_08085"
     CDS             2375..>2625
                     /locus_tag="VFK23_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019624277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HET7319077.1"
                     /translation="MLHSLGAAFRGRSFLHVNSTQEGGGVAEILHRMVPLLTSLGLDA
                     RWEVIKGDARFFDITKKIHNALQGRDESIDERMWSHYEEV"
BASE COUNT          533 a          830 c          761 g          501 t
ORIGIN      
        1 cccgctatct cggcctcggc ctggaaacca taacggcgtc cctgaagggg gaaggagtcc
       61 ccgcaggcgc gtttctgttg aagatcctgt tcacggccat cacgctcagc ttcggcggga
      121 gcggcggaat cattactccc atcttcttca tcggcgcaac cgccggcagc gccttcggca
      181 gggcgctcgg gttcgatccg gccctgttcg cggacatggg aatggtcggc cttctggcgg
      241 gcgcggcgaa taccccgatc tccgcgagca taatggcgat cgaactcttc gggcctgcga
      301 tcgcccccta tgcctcagtg gcctgcgtca tcagcttcct catgacgggc caccggagcg
      361 tttatccgag ccaggttctt tcgctcgcaa aatcctcctc tctcacggtc gccaaaggca
      421 gcgagatgag agacgcggga acgtggaata tcacccccgt cagaaaagcg tcagcagcgg
      481 tatcataaaa ggcctggaaa gactgaaaag atccgggaaa tgactgctgc gccgttccgg
      541 cgcccccttt cgcgcttgct cacttcttca aatctgctac agttatagta tgaaattcct
      601 tcccctgtcc taaaggaggc tccgcgcatg gacatcccct acaaaggcat catcgctccc
      661 gcggcgaccg ctgtcggttt cacgctggcc ctgctcattg tgcgatccgt ggcatttcgg
      721 attttgcacg cgtgggcgaa gcggacggaa acgaagctcg acgacatcat catcgcctcg
      781 acaaaaaatc cctcgctgct gttcgcgctg gcgatcggcg tcaatatcgg gctgtccttc
      841 tccggcatcc ccgagaaaca cctggggtat ctcggcaaga tcctccacgt cgtcatcatt
      901 ctgtccgcga ccatcgcttc ggcgtccatc gtgggcgagc ttttcaggaa ttacgttcag
      961 aagtccaaca tccccatccc gacgacgggc cttgcttacg gcatcctgaa ggggacgatc
     1021 ttcgtcatcg gcttcctgat catcctgacc gatctcggta tctcgattgc cccgctcatt
     1081 accgctctgg gcgtcggcgg tctggccgta gccctggcgc tccaggacac ccttgccaac
     1141 ctttttgccg gcattcatat catgatggaa aagtccataa ggatcggcga tttcgtgaaa
     1201 ctcgagaccg ggcaggaggg gtacgtcgat gacatcacct ggcggaccac gaggatcagg
     1261 atgctgccga acaacatggt cgtcatcccc aacaacaagc tcgcccagag cgtggtcacc
     1321 aattactacc tgccggagaa gagaatgtcg cttacgattc ccgtgagcgt aagctattcc
     1381 tcggacccgg caaaggtgga gcgcatcctg gtcgaggagg cgaaaaaggg agcggcctcc
     1441 ataccgggac tcctggcgga tcccgagccg ttcgtccgct tgattcccgg gttcggcgag
     1501 agttccctgg acttcaccct catctgccag gtccgggaat tcaccgacca gtaccttgcg
     1561 cagcacgagc tccggaagag catcttcaac aggttcaggg aggagggcat cgagatccca
     1621 ttcccgcagc ggacggtgca tctgcgcgag gaaaaggagc aaaaaacatg agcgaaccct
     1681 tcgagttcaa gcagtgcgtc atcctgatca aggccacggg caaaaaggcc ccgggccttc
     1741 gggaactgcg ggaccttatc gccggggcaa gccccgcgtc catctctcac cacaccttcg
     1801 agtactttct gaagggccac accctcgaat acacgaacga cttcgcgcac tgggcgggga
     1861 agagcctgga agagagcgcg ctggcggagc agctctcgaa catcgatccc tatgcctgcc
     1921 gggatgtgga ggaactgcga agcgaactgc tcgaggccat tgacggccat ctcgagcggc
     1981 tgccggagcc gcgggcggcg ctgcccgggg acgagttctt tttcaacgag acggtcgcct
     2041 tcatcgtccc cgcgggtgtc cgcgcaagga acctcgccga attccttatc gcgctgaagt
     2101 atgtcgacga aagctccttg tactaccatt tctacaacgc ccggcagcgc ctgaaaagcg
     2161 gaagggacga attctccgcc tggttcgcga ccctcggcaa ggaggaactt gccgcagcca
     2221 tgaaggccat cgacccctac atgcacaccc tccagggaat ccgggagcgg atcgcggagg
     2281 ctgtggaggc ctccctccgg cgggacatcg agaccgcggg ggtggggccg tgatcgaaca
     2341 atacgcgggg atcgcccccc ggggcgacct ggtcatgttg cacagtctcg gggccgcatt
     2401 ccgcggccgg tccttcctcc atgtcaattc cacacaggag ggaggaggcg tggccgagat
     2461 ccttcaccgg atggtcccgc tcctcacgtc tctcggactg gacgcccgct gggaggtcat
     2521 caagggcgac gcgagattct tcgacatcac gaaaaagatc cacaacgccc tccagggccg
     2581 ggacgaatcc attgatgaac ggatgtggtc ccattacgag gaggt
//
LOCUS       DASSLD010000448         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1532862, whole genome shotgun sequence.
ACCESSION   DASSLD010000448 DASSLD010000000
VERSION     DASSLD010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532862"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFK23_08090"
     CDS             <1..534
                     /locus_tag="VFK23_08090"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET7319078.1"
                     /translation="LWKGGLVFYGGLLAAVPVGIWYMRKHRLPVWKTADIMAPSIALG
                     QAFGRLGCFFAGCCYGATCSLPFGITFNNPDSLAPRGVPLYPTQLMESGGDALIFMTL
                     LWLRRSKKFDGQLFWTYVLFYAVLRFGIEFFRGDEIRGLYFGGSISTSQIIAIGMFTL
                     SGVMMWRLRGAQIANVK"
     gene            616..1563
                     /locus_tag="VFK23_08095"
     CDS             616..1563
                     /locus_tag="VFK23_08095"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET7319079.1"
                     /translation="MPTTTHIVTRKHAGKRLDIFIAHYEPHISRSRIQMMIKDGHALV
                     EGKVEKPGYKVKPDEHVTLELPERQVREVLPEPIPLTVIYEDPHFIVLNKPPGLVVHP
                     APGNYTGTLVNALLYHYGSLPSSGAGAEGGERERAGIVHRLDKDTSGVMVVARTQEAL
                     SALSAQFKSRVVKKRYLALVAGVIKKGSGSVEIGIGRHVKERKKISVHTHRAREAVTT
                     YVVKERYKDATLVEVEIKTGRTHQIRVHMAHIGHPVLGDRVYGGSKAAKSAERQMLHA
                     ESLSLLHPETGNPMTFTVPPPRDMAEVMEKLRRTKRDVA"
     gene            1911..>2738
                     /locus_tag="VFK23_08100"
     CDS             1911..>2738
                     /locus_tag="VFK23_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7319080.1"
                     /translation="MPRQSSIAKITPPRLQNVLLRNRLFDLLDEGRHAPVTWVTGPGG
                     SGKTTLVASWLDAKKLPCLWYQVDEGDGDIATFFYYLGMAAQKAAPRYRRSLPLLTSE
                     YLQDIPTFTRNYFENLYARLKPRIPLHLPLTKEGRRGFATKGEQAGFVIVLDNYQEAP
                     ERSLLHGVVGDALSLIPESIEVFVLSRSEPPSVFMRLRANRSMRLVGWNELRFTLEEA
                     KQIIGKKEKANLSDYAVAELHKKTEGWAAGLILLSEQAGQGDRGPELAGRLAELSRDA
                     "
BASE COUNT          612 a          763 c          780 g          583 t
ORIGIN      
        1 ctctggaagg gcgggctcgt attctacggg ggccttctcg cggccgtgcc agtcggcatc
       61 tggtacatga ggaagcaccg cctcccggtc tggaagacgg cggacatcat ggcgccgtcc
      121 atcgcgctcg gccaggcctt cggaaggctc ggatgtttct tcgccgggtg ctgttacggc
      181 gcgacgtgca gcctgccttt cggcatcacc ttcaacaacc ctgattcgct ggccccccgg
      241 ggagtgcctc tctatccgac gcagctcatg gagtcgggag gcgacgccct catcttcatg
      301 acgctcctct ggctccgccg ctcgaagaag ttcgacggcc agctcttctg gacctacgtc
      361 cttttctatg ccgtcctccg gtttggtatt gagtttttcc gcggggacga gatccggggc
      421 ctgtatttcg gcggctcgat ctcgacttca cagatcatcg caatcggcat gttcacgctc
      481 tcgggagtca tgatgtggag gctcagaggg gcgcagatcg cgaatgtaaa ataagaaaga
      541 tcggaataca gaagacagaa tactgaggac ttctgactcc tgtctcctga cttctgactt
      601 ctggtatttc ttcccatgcc taccacgacc cacatcgtca ccaggaagca cgccggcaag
      661 cgcctcgata tattcatcgc gcactacgaa ccgcacatct cccgaagccg cattcaaatg
      721 atgattaaag acggccatgc ccttgtcgaa ggcaaggtcg agaaaccggg atacaaggtg
      781 aagccggacg agcatgtcac cctcgaactg ccggagcggc aggtccgtga agttctgcct
      841 gaacccatcc cgctcaccgt catctatgag gacccccatt ttatcgtgct gaacaagccc
      901 cccggactcg tggtgcatcc ggccccgggc aactacaccg gaaccctcgt gaacgccctt
      961 ctctatcact acggcagcct gccgtcatcg ggcgcgggag cagagggggg tgagcgggag
     1021 cgcgcgggca ttgttcaccg gctcgacaag gacacctcgg gcgtgatggt ggtcgcgcgg
     1081 acccaggagg cgctcagcgc gctgtcggcc cagtttaaaa gccgtgtcgt caaaaagaga
     1141 taccttgccc tcgtggcggg agtgatcaag aaagggtcgg gcagcgtcga aatcggtatc
     1201 ggcaggcacg tgaaggagcg aaagaagatc agcgtccaca cccaccgcgc gcgggaagcg
     1261 gtcacgacct acgtcgtcaa ggaacggtac aaggacgcca cgctcgtgga ggtcgagatc
     1321 aagacgggaa gaacgcacca gatacgggtg cacatggcgc acatcggcca ccctgtgctg
     1381 ggcgaccgcg tctatggcgg aagcaaagcg gcgaagtccg cggaacgcca gatgctgcac
     1441 gctgaatcgc tctcgctgct ccatccggag acggggaacc ccatgacatt caccgtcccg
     1501 ccgccccggg acatggctga ggtgatggaa aagctccggc gaacaaagcg ggatgttgca
     1561 tagacaaaag gtttgaactt agacggttgc ttttcgcctt aagatttgac cttcgcaccc
     1621 ttctgcacca cattttaatt tttcataaag aaatctgcca caagtacaaa taagagtggg
     1681 aggacattgc cacagagata cttacaaaaa accgaaacaa ttttttttgt aaaaaagtgt
     1741 tgcccgttgt ctcgaatact gttatagtat attaactttg gtgtgatatt ttcttatttt
     1801 tagtacaccc cggaaacctc tttcagcgag tctgcctttc atatgctttg atggcgtttt
     1861 tttatcgtat cctgtgtcga taactcgctt tattggcatc ttgcgataaa atgcccagac
     1921 aatcgtcaat agcaaagatc acccccccgc ggctccagaa cgtgctgctc cgtaataggc
     1981 tgttcgatct cctggatgag ggacgccatg cgcccgtcac atgggtcacg ggcccgggag
     2041 gttcgggcaa aaccacgctc gtcgcgagct ggctcgatgc gaagaaactc ccctgcctgt
     2101 ggtaccaggt ggatgagggc gacggggaca tcgcgacttt cttctactac ctgggcatgg
     2161 cagcacaaaa ggccgcgccc cgttatcgaa gatcccttcc tctacttacc tccgaatatc
     2221 ttcaggacat tccgactttt acacggaatt attttgaaaa tctttacgcc agactaaaac
     2281 cccggatccc tcttcacctc cctttgacaa aggagggcag acggggattt gctacaaagg
     2341 gcgagcaggc gggatttgtg attgtattgg acaattacca ggaggcgccg gaacgatcgc
     2401 tcctccacgg ggtcgtaggc gacgctttat cgctcattcc ggagagtata gaggttttcg
     2461 tactgagcag gagcgagccg ccttcggtct tcatgagatt gcgcgcgaat aggtcgatga
     2521 ggctggtcgg atggaatgag ctccggttta ctttggagga agcaaagcag atcatcggta
     2581 agaaggaaaa ggcgaatctc agcgattatg ccgttgcgga gctgcataag aagacggaag
     2641 ggtgggccgc cgggttgatt cttctctcgg agcaggcagg gcagggagac aggggacccg
     2701 aattggcagg cagacttgcc gagctttcac gggacgct
//
LOCUS       DASSLD010000449         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_487193,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000449 DASSLD010000000
VERSION     DASSLD010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487193"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFK23_08105"
     CDS             complement(<1..279)
                     /locus_tag="VFK23_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET7319081.1"
                     /translation="MKKLVSVLSTLLLLAATGCTFLKVSLTEEVHPLTEEVIGGSGRN
                     KVLLLDISGFISDRDGNLPGGKETGLLARVREALDRAREDGRVKAVVLR"
     gene            complement(412..1113)
                     /locus_tag="VFK23_08110"
     CDS             complement(412..1113)
                     /locus_tag="VFK23_08110"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET7319082.1"
                     /translation="MMPPARLRKTAAVLTLIVAVLLWSPAAPAEVLDHIAAAVNNEVI
                     TASELADAVALNERLGTPGTDKQTLESETLEGLITRRLLVQEARRLRFVEVSEQDKAA
                     EVDKVKARFASDAAFADFLRALDMTEQELAAMLGERLLVEKFVEKKVGLFVRVTRSEA
                     EAYFREHAAEYPGQNFQDVQKNIVAILTDRKVGQQLDQYVADLRGRANIRVNRLPEAA
                     VQDSLGPAIIDRVNQ"
     gene            complement(1110..>2434)
                     /locus_tag="VFK23_08115"
     CDS             complement(1110..>2434)
                     /locus_tag="VFK23_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7319083.1"
                     /translation="LWKGAAEISRGTLDYQMQLSGTSDVDQLAERFNEMAVKLKNSYA
                     DLEKKLLDRTNELAAIDAVALTLSRAGNLPDMLSRSLDQIMDNMGGLEPRGGVFLCEP
                     DGETLRLVAHKGLPPGFIEQEGTIRMGECLCGMVAQSGEILYSERGCEDFRHTRGQEG
                     TVSHSHIIIPIKSRGIVFGVIFLYPQKEFSPRPSDLQMLDTIGTQLGMAVENLRFYAE
                     VKESSEKFWDLFEYSRDILFTTDGSGVLTAVNKEAEQFSGYSKTELVGKNVLDFLTPE
                     GAEAARRLLGGENVTPSQVMEFEVVRRDGTRAFVELSARRLSGQGTPAGYQISARDIT
                     EQKLMREKLVRAERLGAIGEIVIAVRHEVNNPLTTVIGNIELLIERYGDRDKDLASRL
                     EVVLSNALRIAEIVKQLQAIKRDKVVEYVRGVTMTDLRQGSGEETRLP"
BASE COUNT          434 a          775 c          709 g          516 t
ORIGIN      
        1 ccggagcacg acggccttga cccttccgtc ctccctggcg cggtccagcg cctcccggac
       61 acgggcaaga aggccggttt cctttccgcc cgggaggttg ccgtcacggt ccgagatgaa
      121 gcccgagatg tcaaggagca agaccttgtt ccggccactg ccgccgatca cctcttcggt
      181 gaggggatgc acctcctcgg tcagcgaaac cttgaggaac gtgcagccgg tcgcggcgag
      241 cagcaggagc gtggaaagaa ccgaaacaag ttttttcacg gagcctccta aacaaaacat
      301 tgcattgcct attggtatga acataacaaa aaaatttgcc acaaaaaggc gcaagaagca
      361 caaagaatga atttcttgtg ctttatgtgc ttccttgcgg caagaaagtt tttattgatt
      421 tactctgtca attattgctg gcccgaggct gtcttgcact gcagcttcgg gaagcctgtt
      481 gaccctgatg ttggccctgc cgcgcaagtc cgcgacgtac tggtcgagct gctgtccgac
      541 cttccggtcc gtcaggatcg ccacgatgtt tttctggacg tcctgaaagt tctgcccggg
      601 gtactcggcg gcatgctccc tgaaataggc ttcggcctcc gaccgcgtta cccgcacgaa
      661 gaggccgacc ttcttctcga cgaatttttc caccaggagc cgctccccga gcatggcggc
      721 aagctcctgc tcggtcatgt ccagggcgcg caaaaaatcg gcgaaggccg cgtccgacgc
      781 gaagcgggcc ttgaccttgt cgacctccgc cgctttatcc tgctccgaga cctccacgaa
      841 ccggagccgt cttgcctcct ggaccaaaag tcttcgcgtg atgagccctt cgagtgtttc
      901 ggattcgagc gtctgtttgt ccgtgccggg cgtgccgagg cgctcgttca gcgcaacggc
      961 atcggccagc tcgctcgccg tgatcacctc gttgtttacg gccgcggcga tgtggtccag
     1021 gacctcggcc ggagccgcgg gtgaccatag gagaaccgca acgatgagtg tgagcaccgc
     1081 ggcggttttt cgtaaccttg cgggaggcat catggtaatc gcgtctcctc cccgctgccc
     1141 tgcctcaggt ccgtcatcgt gacgcccctg acatactcca cgactttatc gcgcttgatg
     1201 gcctggagct gcttcacgat ctcggcgatg cggagtgcat tgctgagaac gacctcgagc
     1261 cgcgacgcga ggtccttatc cctgtctcca tagcgttcga tcaggagctc gatgttgccg
     1321 atgacggtgg tcagcgggtt gttgacctcg tgacgcaccg cgatcacgat ctctccgatc
     1381 gcgccgagcc gctccgccct tacgagcttt tccctcatca gcttctgttc cgtaatgtcc
     1441 cgggctgata tctggtagcc tgcgggcgtc ccctgcccgg agagcctgcg ggcgctcagc
     1501 tcgacgaagg cccgggtccc gtcgcgcctg accacttcaa actccatcac ctggctgggg
     1561 gtgacgttct caccgccgag cagccgtcgg gccgcctcgg cgccttcggg cgtcagaaag
     1621 tcgagcacgt tctttccgac gagttcggtc ttactgtagc cggaaaattg ttccgcctcc
     1681 ttgtttaccg ccgtcaggac gccggagccg tcggtggtga agaggatgtc ccgggagtac
     1741 tcgaaaaggt cccagaactt ctcgctcgac tccttcacct cggcgtagaa ccgcaggttc
     1801 tcgactgcca tgccgagctg ggtgccgatg gtatccagca tctggaggtc cgacggccgg
     1861 ggggagaatt ctttctgggg atacaggaag atgacgccga aaacgatgcc gcgcgacttg
     1921 atcggaatga tgatgtgcga atgggaaacc gtcccctcct gaccgcgcgt atgccggaag
     1981 tcctcgcacc cccgttccga gtacagaatc tcgccgctct gggccaccat gccgcagagg
     2041 cactcgccca tcctgatcgt gccctcctgt tcgataaacc cgggcgggag gcccttatgc
     2101 gccacgagac ggagggtctc tccgtcgggt tcgcagagaa acacgccccc ccgcggttcc
     2161 agcccgccca tgttatccat gatctggtca agggaccgcg agagcatgtc cgggagattg
     2221 cccgcccggc tcagtgtcag tgcaacagcg tcgattgcgg caagctcgtt cgtccggtcg
     2281 agcagctttt tctcaaggtc ggcgtaggag ttcttgagct tcacggccat ttcgttgaag
     2341 cgctccgcaa gctggtcaac atcgctcgtc cccgacagct gcatctggta gtcaagggtg
     2401 ccccggctga tttccgcggc gcccttccac agtc
//
LOCUS       DASSLD010000450         3931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_173104,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000450 DASSLD010000000
VERSION     DASSLD010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173104"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VFK23_08120"
     CDS             <1..1124
                     /locus_tag="VFK23_08120"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET7319084.1"
                     /translation="VDLYGRETWLANQPASKLPFSVPGASDSIPTSQTQFLTYGVKAT
                     QILYDFGKTSSSVGAAEYGLKSREIEIDRVKNQAALDFIVAYLDLLESEKLLQVSKEE
                     VQQYEAHQKDTQSRFAAGVVTKNEVLQAEVTLADSRQRAVSADNLRSLRESRINSLLL
                     RPLNEQVKPEEISASPSTGLTLEEAWASAEETSNRLKEIDQVIAAKEENIKSLRAEYM
                     PTFYLSGGYEYSENKYLVHQDNWSAIAGVNVNLYSGGASNSRVGLAKAELRSLKLSRD
                     KLLDGIRLEVKAAYLDLQSSAQKMEVTQAAVDQAVENLRLQRLRYREGVGTATDVLDA
                     VTLLSTAETNSWKALYGMKRAEASLLNASGKDLVSMYGK"
     gene            1280..2284
                     /locus_tag="VFK23_08125"
     CDS             1280..2284
                     /locus_tag="VFK23_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET7319085.1"
                     /translation="MAEVANNNNGKKKKAFLIVGVIVAIGLVSGWTYNGYRKTHVSTD
                     DAFVEGNIHTIAAKIYGTVKAIRINDNQAVKKGDLLLEIDPSDYDVRLREATSALGVE
                     KAKLAEVETRIESARANLDLARANLKLSETDKSRAEKLMTEQVIPQERYDRAITGYDI
                     ARAQLKAAEEQLRQAESQKVTQASTIRQKEASAALANMNYEYTKIFAPVDGYVTRRSV
                     QVGNQIQPGQPLMALVSLNDIWVIANYKETEMGNIRPGQDVEIKVDSFPGRVFKGKVD
                     SIMAGTGVSFSLFPAENATGNYVKVVQRIPVKIVLDENADKDHVLRIGMSVVPTVIAK
                     "
     gene            2284..3825
                     /locus_tag="VFK23_08130"
     CDS             2284..3825
                     /locus_tag="VFK23_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020879225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET7319086.1"
                     /translation="MHKTQSRGIIAFTVMLPTLIEIIDTSIVNVSLDHIRGSLSAGYD
                     EATWTITAYLVSNAIVIPMAAWFAKLIGRKNYQIASIALFTFSSFMCGSAWSLGSLVF
                     FRVLQGIGGGGLVPISQSILLESFPREKHGQAMAIFGLGAMLGPIVGPLLGGWITDSL
                     SWRWIFYINIPIGIIAIIMNILVINDPPYMKRQKMRIDYWGLLFLSVGLGALQFLLDK
                     GESEDWFESRLIITVGVVSAVSLVLLVINEYYSEHPIVNLKLFKDRTFTSGATVMFFV
                     FLNLFGSIVLLPIFLQMMMGYTSFYAGLVLGPGGIATMFAMPIVGKFIGRVNPKRFLI
                     LGIIVCALSTCMMSRFNLTTDFWAFVWPRVLLGFGMGMTFIPLTTLTLSHIPKERMTE
                     ATSLYNLLRNLGGSVGIAFTTTILSRRAQIHQTRLVEHLSPFDPAYSTYRDKIASFLT
                     AKGLPATGADGMMYRELIRQSMTLAFTDAFMIICILILSVLPLVFIMQWQKTPGPGGP
                     PVDAH"
BASE COUNT          883 a         1156 c         1076 g          816 t
ORIGIN      
        1 ctgtcgatct ttacggacgc gagacatggc tcgcgaacca gcccgcgtcc aagcttcctt
       61 tcagtgtccc gggcgcgtcc gattcgatcc cgacttcgca gacccagttt ctgacctacg
      121 gggtaaaggc cacacagatc ctctacgatt ttggaaaaac ctcatcctcg gtgggcgctg
      181 cggagtatgg gttaaagtcc cgggagatag agattgaccg cgtgaagaac caggccgccc
      241 tggactttat cgtcgcctat ctggacctgc ttgaatccga gaagctgctc caggtttcga
      301 aagaggaagt acagcagtac gaggcccacc agaaggacac tcaatcccgg ttcgccgccg
      361 gcgtcgtcac gaaaaacgag gtgcttcagg cggaggtgac gcttgctgac tcgcggcagc
      421 gcgctgtttc tgccgataac ctccggtccc tccgggaatc gaggatcaat agcctgcttc
      481 tgcggcccct gaacgaacag gtcaagcccg aggagatatc ggcgagcccg tcaaccggcc
      541 tgacgcttga agaagcgtgg gccagcgcgg aagagacgag taaccggctt aaagagatag
      601 atcaagttat cgcggcaaag gaagaaaaca tcaaatccct gcgggccgaa tatatgccga
      661 cgttctatct ttcgggcggg tatgagtatt ctgagaacaa gtaccttgtc catcaggaca
      721 actggtctgc catcgcggga gtgaacgtca atctctattc cggcggagcg agcaactccc
      781 gggtcggttt ggcgaaagca gaacttcgct cgctcaagct ctcccgggac aagcttctcg
      841 acggcatacg actggaagtt aaggccgcct atctcgacct tcaaagttct gctcaaaaga
      901 tggaggtcac tcaggctgca gtcgaccagg ccgtggagaa cctgaggctt cagaggctcc
      961 ggtaccggga aggcgtcggc accgccacgg acgtgctcga cgcggtaaca ctcctcagca
     1021 ccgcggaaac gaattcctgg aaggcgctct acgggatgaa gcgtgcggaa gcgtccctcc
     1081 tcaatgcctc gggtaaggac ctggtaagta tgtatgggaa gtaaccgata agcactaaat
     1141 aacaaatccc aaattccaaa taaattctca gcacgaaatt taaaattcga ctgtttggaa
     1201 cgttggcttt tttaaatttg aagtttattt gggatttggt gcttggaaat ttggaaattt
     1261 catttgggga gttgatagca tggcggaagt agcgaacaac aataacggaa aaaagaagaa
     1321 agccttcctg atcgtcgggg tgatcgtggc catcggcctc gtctcgggat ggacctacaa
     1381 cggttaccgc aagacccacg tttcgacaga cgacgccttc gtcgagggga acattcatac
     1441 gatcgcggcc aagatatacg ggacggtgaa ggcgatacgg ataaacgaca atcaggccgt
     1501 gaagaaaggg gacctccttc tcgaaatcga tccctccgat tatgatgtga ggctccggga
     1561 agccacctca gcgctgggcg ttgaaaaggc gaaacttgcg gaggtcgaga cgaggattga
     1621 gtcggcacgg gcgaaccttg atctggcaag ggcgaatctc aagctgtcgg aaacggacaa
     1681 aagccgtgcg gaaaagctca tgacggagca ggtcattccc caggagcgct atgaccgggc
     1741 gataacgggc tatgatatcg cgcgcgccca gctcaaggcg gcggaagaac agctcaggca
     1801 ggcggaatcg cagaaggtca cgcaagcgtc gacgatccgg cagaaagagg cctcggccgc
     1861 gctcgccaac atgaattacg aatacaccaa gatcttcgcg cccgtggacg gatacgtcac
     1921 gagaaggtcc gttcaggtcg ggaaccagat ccagccgggc cagccgctca tggccctggt
     1981 ctcgctcaac gacatctggg tcatcgcgaa ttacaaggaa accgagatgg gaaacatccg
     2041 tcccggccag gatgtggaga tcaaggttga ctcattcccc ggcagggtct tcaagggcaa
     2101 ggttgacagc atcatggcgg ggaccggagt atccttctcg ctcttcccgg ctgagaacgc
     2161 cacgggcaac tacgtgaagg tggtccagag gatacccgtc aagattgtgc tcgatgaaaa
     2221 cgccgacaag gaccatgtct tacggatcgg catgtccgtg gtgccgaccg ttatcgcgaa
     2281 ataatgcaca aaacccaaag ccgcggcatc atcgccttca cggtaatgct gccgactctg
     2341 atagaaatca tcgacacgtc catcgtgaac gtgtctctgg accacatccg gggctcgctc
     2401 tccgccggat acgacgaggc cacgtggacc atcacagcct acctcgtctc gaacgccatc
     2461 gtcattccca tggcggcctg gttcgccaag ctcatcggca ggaaaaacta ccagatcgcc
     2521 tccatcgccc tcttcacctt cagctccttc atgtgcggct cggcctggag cctggggagc
     2581 cttgttttct ttcgggtact ccaggggatc ggtggaggcg ggctcgtgcc cataagccag
     2641 tccatcctgc tcgaatcctt cccgcgcgag aaacacggcc aggccatggc catcttcggg
     2701 ctcggcgcca tgctcggtcc catcgtcggc ccgcttctgg gcggctggat aaccgacagt
     2761 ctctcctggc gctggatctt ctacatcaac atcccgatcg gcatcatcgc gatcattatg
     2821 aacatcctcg tgatcaacga cccgccgtac atgaagcggc agaaaatgag gatcgactac
     2881 tgggggcttt tgtttctttc cgtgggactg ggcgcactcc agttccttct cgacaagggt
     2941 gagagcgagg actggttcga atcgcgcttg atcattaccg tcggcgttgt ctccgctgtt
     3001 tcgctcgtcc tgctcgtgat caacgagtac tattccgaac acccgatcgt gaacctgaaa
     3061 ctgtttaagg accggacgtt tacttccggc gctacggtca tgttcttcgt gttcctgaac
     3121 ctcttcggga gtatcgtgct tctgccgata ttcctccaga tgatgatggg gtacacatcg
     3181 ttttacgccg ggctcgtact tggccccgga ggcatcgcga ccatgttcgc catgcccatc
     3241 gtgggaaagt tcatcggcag ggtgaatccc aaacggtttc tcatactcgg catcatcgtc
     3301 tgcgcactct ccacctgtat gatgtcccgg ttcaacctga cgaccgattt ctgggccttt
     3361 gtctggcccc gcgtccttct gggcttcggt atgggcatga cgttcatccc gcttacgacg
     3421 ttgactcttt cgcacattcc caaggaacgg atgacggagg ccacgtcgct ctacaatctc
     3481 cttcggaacc ttggcgggag cgtgggcatc gcctttacga ccaccatcct ctcccgcagg
     3541 gcccagattc accagacgag gctcgtggag cacctgtcgc ccttcgaccc cgcttactcc
     3601 acctaccggg acaagatcgc ttcctttctc acggcaaaag gcttgccggc cacgggcgcc
     3661 gacggcatga tgtaccgcga gctcatccgc caatccatga ccctcgcctt caccgacgcg
     3721 ttcatgatca tctgcatcct catcctctcc gtgctcccgc tcgtcttcat catgcagtgg
     3781 caaaagaccc ccggccccgg cggtccaccc gttgacgcgc attaatcctc tcaccacaaa
     3841 aaaagaggat gacgttaccg ccatcctcac agttccgcta ccttctcagt ccgcttagtc
     3901 aactgcaaat cccggcgctt cattgaagaa g
//
LOCUS       DASSLD010000451         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1046498, whole genome shotgun sequence.
ACCESSION   DASSLD010000451 DASSLD010000000
VERSION     DASSLD010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046498"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFK23_08135"
     CDS             <1..223
                     /locus_tag="VFK23_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HET7319087.1"
                     /translation="FKDPKDYELLTKGTKVVFPDVQSRIARGDREIPAEVDGKTIMTI
                     LDVSERQRKHLLAGGTLNYVKKELLRKAA"
     gene            442..1623
                     /locus_tag="VFK23_08140"
     CDS             442..1623
                     /locus_tag="VFK23_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HET7319088.1"
                     /translation="MSEALNVKNTGLRGVAIADTKISNTDGENGILIYRGYRIEDLAK
                     NSTFEETAYLLLYDALPNEEALRDFKRELTEAGELPSFVFDALRKMPKTAQPMDVLQA
                     SIPLLASADPDLRMDTREANISRAIRLIARVPMIVAGWDRLRRGHEPLPTDEKLSHAG
                     NFLWRLKGNYPDAEIERDLDTALLLHADHSFNASTFACREVVSTRAHMYAGVAAGVGA
                     LSGSLHGGANAEVMKMLQELEHEKDVAGWVKKELDAGRRIMGMGHAVYKTFDPRAQFL
                     KDMCSRIGEKTGQEQWCRLSAQVEEAALLEFEKRGKTTIKPNVDFYSAPVYHMMGIPH
                     DLMTPVFAVARIAGWTAHIIEEKFGDAQGKPMLYRPSSVYVGNYCGKTGCVYEPLERR
                     K"
     gene            1821..>2059
                     /locus_tag="VFK23_08145"
     CDS             1821..>2059
                     /locus_tag="VFK23_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319089.1"
                     /translation="MATRKATVQKRKVKKGTARPAVKRVSSGQGVKKMKANTLKSVVK
                     RPAKIQSINPYTEQVMQEFPLMSRQEVDWQLKASH"
BASE COUNT          504 a          545 c          632 g          378 t
ORIGIN      
        1 ctttaaagac ccgaaggact atgaactcct cacgaaggga acgaaggtcg tattccccga
       61 tgtgcagtcg cggatagccc ggggagaccg ggagataccc gcggaggtcg acggcaagac
      121 gatcatgacg atcctggacg tttccgaacg ccagagaaaa catctccttg cgggagggac
      181 tttgaattac gtgaagaaag agttgctgag aaaggcggca tgaacgcaac aattttcctc
      241 cccttcatgg ccgataaaac gggccacgct gaggggagga tcaaggaggg gatgggactc
      301 tggaatcggc attaaaccca tcaccccagc cctctccttg aaggagaggg tgttcaatag
      361 cggccggatt ccgcgctatg aaaggttgtc actatgggta cgatttctca cataaaggat
      421 tctcagaaaa gggaggctaa gatgtcggag gccttgaatg taaagaacac cggtcttcgc
      481 ggcgtggcaa ttgcagacac gaagatatcc aataccgacg gcgagaacgg tattctcatc
      541 taccgtggat accggatcga ggatctcgcc aaaaactcga cgttcgagga gacggcatac
      601 ctgctcctgt atgacgcact gccgaatgag gaagcactca gggatttcaa gcgcgagttg
      661 acggaggcgg gggaactccc ttctttcgtc ttcgacgcct taaggaaaat gccgaagacc
      721 gcgcagccca tggatgtgct tcaggcctcg atcccgctcc tggcctcggc tgatcccgat
      781 ctgcgcatgg atacgcggga ggcgaacatc tcccgggcca tcaggcttat cgcgcgggtc
      841 cccatgatcg tcgccggctg ggaccggctg cggagagggc atgaaccgct gccgaccgat
      901 gaaaagctgt cccatgccgg gaactttctg tggcgcctca aggggaacta tcctgatgcg
      961 gagatcgagc gcgaccttga tacggcgctc ctgctccatg ccgatcattc cttcaatgcc
     1021 tccactttcg cgtgccgcga ggtcgtatcg acccgcgctc acatgtacgc gggtgttgcc
     1081 gcgggtgtgg gggccctttc gggttctctc cacggcgggg ccaacgccga ggtgatgaag
     1141 atgctccagg aactggagca tgaaaaggac gtcgccgggt gggtgaaaaa agagctcgac
     1201 gccggccgca ggatcatggg tatgggccac gcggtataca agacgttcga tccgcgagcg
     1261 cagttcctca aagacatgtg ctcccggatc ggagagaaaa cgggtcagga acagtggtgc
     1321 aggctgtcag cgcaggttga ggaggccgcc ctcctcgagt tcgaaaaacg gggcaagacc
     1381 acgatcaagc cgaatgtcga cttctacagc gccccggtct accacatgat gggcataccg
     1441 cacgatctga tgacgccggt cttcgctgtg gcgcggatcg ccggctggac cgcgcacatc
     1501 atcgaggaga agttcggtga cgcgcagggg aagcccatgc tgtaccgccc gtcgtccgtg
     1561 tatgtgggca attactgcgg aaagaccggc tgcgtctatg aaccgctcga gcggaggaag
     1621 tgagtcgaat gctgaatgcg gatttcggat ggccgatgcg acgggccacg ctgatgcgga
     1681 ataaaagtac ggcaacactt tttcgtcatc cccgaatggt tctatcggga atccatgttt
     1741 tcaaaataat gcgaaatcaa agtcgctgga ttccggctta aggcatgccg gaatgacgga
     1801 taaatttgcg gaggacaccg atggcgacca gaaaagcaac ggtacagaaa agaaaggtaa
     1861 aaaagggcac agcccgcccc gcggtgaagc gcgtgtcctc cgggcagggg gtaaagaaaa
     1921 tgaaagcaaa tacactgaaa tcggtcgtaa agcgaccggc gaagatacag tcgatcaatc
     1981 cttacaccga acaggtgatg caggaattcc cgctcatgag caggcaggag gtcgattggc
     2041 agctcaaggc ctcccatga
//
LOCUS       DASSLD010000452         3927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_473479,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000452 DASSLD010000000
VERSION     DASSLD010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473479"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..920)
                     /locus_tag="VFK23_08150"
     CDS             complement(117..920)
                     /locus_tag="VFK23_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HET7319090.1"
                     /translation="MPAIITLTTDFGTRDSFTASVKGVILKINPQAQIIDISNEISPQ
                     DVWEAAYVLKSAYSHFPKGTIHLAVVDPGVGSGRRPIIAVTESYYFVGPDNGLFSLVY
                     HDAERLRVHHITSTHYFLPNPGPTFHGRDIFAPVAGWITKGIPSGNFGEEITDFARLN
                     VPAPKVGENGAEGRVVHIDRFGNIITDILFKDVQTLLAGGSGEGSVTVLLGGREIKGL
                     KRFYAEAAPGEPGVIFNSNGALEIFLFKQNARNALSVKRGEVVRLTINR"
     gene            complement(1015..2367)
                     /locus_tag="VFK23_08155"
     CDS             complement(1015..2367)
                     /locus_tag="VFK23_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319091.1"
                     /translation="MVRRIWGLVLILAVGACSAANALADDLHYNNILIGDRASNMGGA
                     YTAVSDDATGLYYNPAGIVYSAGRSVSANVNGYNYSKKTYKEVIGGNGWVRNSAALLP
                     NYFGVVMPMGEDYKIGFSYALPDSITSDQEQTFHDLQLSAAVQPFNPGVTITSYLINF
                     NEESNTYNIGPSIAKEFSDTFSAGLTLYYYQRKTLRILNQIIKTSNGGSEQSTDFFHS
                     TESGVRPVLGFMWSLPKNVAVGLAFSKVFIAPTADLGFAKIRIGESKTTFHTSDQRVN
                     IAQDSDPTHNTAISLPAGPSNSDAKRTTPKQMSLGVAWFPSSSLLLSADLNFYTAKSV
                     GDIVQFKASSEPLADVVNVALGTEYYITKNWAARAGFYTDFANTPKIAEGGINQAEHI
                     DNYGVTASISHFTRNTSVTLGTGFTYGTGQAQIIGDSTSIQEAESRGWMINLSSTYAY
                     "
     gene            complement(2399..3250)
                     /locus_tag="VFK23_08160"
     CDS             complement(2399..3250)
                     /locus_tag="VFK23_08160"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET7319092.1"
                     /translation="MIKPAVTMTVVLLMLSVLCPSSIPAAQPEENWRDRISTEAGVVA
                     TSDIVEEIRFGRAVAARIIGRYGLYENTQIMIYINLVGQTIARGANRPEIEFHFAVLN
                     GDEIMAFGIPGGYIFITKGALRAMRDEAELAGILAREVGHIAEKTAVRELKIRGIEDA
                     GITGLAQIIGGSTDPARAAFRKAVDKAMDMLFRKGYKREEDEKADEDAVILCAMAGYD
                     PSGLVRYMGRTHGVAGKQTGAIDGAQTSIEVRIDRLKAFILQEGADAGDFKTYEKRFI
                     KYMSAVR"
     gene            complement(3247..3732)
                     /locus_tag="VFK23_08165"
     CDS             complement(3247..3732)
                     /locus_tag="VFK23_08165"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET7319093.1"
                     /translation="MAKTIVITVFIVVALWHAPALHAADEYYVQSVKAKVMSGPTFKS
                     AVLGEVDKGYKFISSGKEGGWTKVKYNNGDGYVHSFVLLMHPPKESVWAITADEQELR
                     RGFRRRAASATGIEADRGLDTDVEKSERKGDRVDYESLQEIELFTMSYSEIIQFMEGK
                     E"
BASE COUNT          808 a         1108 c         1066 g          945 t
ORIGIN      
        1 gttcggaatc gtgttcgttc atctggcatc cgaaggttat gatatggtac gatttcatgg
       61 aagaaatatt ataatacgga ggggattttg aaacaagcga tatttcgaag gaatgtctaa
      121 cggtttattg tcagccggac cacttctccc cgcttcacgg acagggcgtt gcgggcgttc
      181 tgcttgaaca agaatatctc gagtgccccg ttgctgttga aaattacccc tggctccccg
      241 ggagccgctt cggcatagaa tctcttgagg cccttgattt ccctccctcc caacaggacc
      301 gtgacgctgc cctcaccgga tcctccggca aggagggtct ggacgtcttt gaacagaata
      361 tcggtgatga tgtttccgaa gcggtcgata tgaacgacgc gcccttctgc cccgttctca
      421 cctactttcg gggcggggac attaagcctc gcgaagtccg taatttcctc gccgaagttg
      481 ccggaaggaa tgccctttgt aatccatccc gcaacgggag cgaagatgtc cctgccgtgg
      541 aacgtggggc ccgggttcgg aagaaaataa tgggtcgagg tgatatggtg cacccgaagg
      601 cgttcggcgt cgtggtaaac gaggctgaaa aggccgttat ccgggcctac gaaataataa
      661 ctttccgtca cggctatgat cggcctcctg ccgctcccta cgccgggatc gacgacggcg
      721 aggtgaatcg tcccttttgg gaaatgactg tacgcggact tcaggacgta tgctgcttcc
      781 cagacgtcct gcggagatat ttcattcgag atatcgatga tttgagcctg agggttgatc
      841 ttgaggatga cgcctttgac gctcgctgta aaggagtccc gcgtcccgaa atcggttgtc
      901 aacgttataa tggcgggcat caatcccccg gaatgaattt caggctgaaa actgcgagaa
      961 taatatcaac catcgtgtgc tttgtcaact tattcagcag ggattcgcga ctgctcagta
     1021 ggcataggtc gaggagaggt tgatcatcca tccccgggat tcggcctcct ggatcgacgt
     1081 actgtcgccg atgatctgag cctggcccgt accataggtg aagccggtgc ccagcgtcac
     1141 tgatgtgttt ctcgtaaagt ggctgatgct cgccgtgacc ccgtagttgt cgatatgctc
     1201 ggcctggttg attcctccct cggcgatctt cggtgtgttg gcaaagtccg tatagaagcc
     1261 ggcccgagcg gcccagtttt tcgtgatata gtattccgtg ccgagcgcga cgttcacgac
     1321 gtctgccaga ggttccgaac tggccttgaa ctgaacgatg tcgccgacgc tctttgcggt
     1381 gtagaaattg agatccgcgg acaacaggag actcgatgaa gggaaccatg caactccgag
     1441 gctcatctgc ttcggcgtcg tacgctttgc atccgagttc gaggggcccg cgggaaggga
     1501 aatggcggta ttatgggtgg gatcactgtc ctgcgcgatg tttacccttt gatcgcttgt
     1561 atggaaggtg gtcttggact ctccgatccg aatcttagca aatccgagat cggccgtcgg
     1621 tgcgatgaat accttcgaaa atgccaggcc cactgcgacg ttctttggca gcgaccacat
     1681 gaaccccaga acgggcctca cgcccgattc cgtggagtga aaaaaatcgg ttgattgttc
     1741 ggagccgccg ttgcttgtct tgatgatttg gttcaggatg cgcagggtct tccgttggta
     1801 atagtacaag gtaagtcccg cagagaaggt atcggaaaac tccttcgcaa tcgacggccc
     1861 gatattatag gtattgctct cttcgttgaa gttgatcaga tacgatgtaa tcgtgacgcc
     1921 ggggttgaac ggctgcactg ccgcgcttag ttgaagatca tggaacgtct gctcctggtc
     1981 cgaagtgatg gaatcaggca aggcgtagga aaaaccgatt ttataatctt ctcccatggg
     2041 catgaccacg ccgaaatagt tcggcagaag cgcggccgag ttacgcaccc aaccattgcc
     2101 gccgatgact tccttgtacg tttttttcga gtaattatac ccattgacgt ttgccgatac
     2161 gctcctgccc gccgaataga cgatgcccgc aggattgtag tagagtcccg ttgcgtcgtc
     2221 ggagacagcg gtataagcgc cacccatgtt cgatgcccgg tctccgatga ggatattgtt
     2281 atagtgcaga tcatcggcaa gggcgttcgc ggcgctgcat gcaccgacgg ccagaatgag
     2341 aaccaatccc catattcttc gaaccatgcc acccccagtt cagcatccgt gaggcgattt
     2401 atcttacggc gctcatatac ttgatgaacc gtttctcgta ggtcttaaag tcgcccgcgt
     2461 cggcaccctc ctgcaggatg aaggccttga ggcggtcgat gcggacttcg atggaggttt
     2521 gtgccccgtc aatcgctccg gtctgttttc ccgccacgcc gtgggttcgg cccatatacc
     2581 tgaccagccc cgatggatca tatcccgcca tggcgcataa aatgaccgcg tcctcgtcgg
     2641 ccttctcatc ttcttccctt ttgtaaccct ttctgaagag catgtccatg gctttgtcca
     2701 cggcctttcg aaaggcggcc ctcgcgggat cggtgctgcc gccgatgatc tgcgccagtc
     2761 cagtgatgcc cgcatcctcg atgccgcgga tcttgagctc cctgactgcc gtcttctccg
     2821 cgatatgtcc cacttcacgg gcgaggatgc cggcaagttc tgcctcgtca cgcatcgcgc
     2881 gcaaggcccc tttcgtaatg aagatatagc ccccgggaat cccaaaggcc attatctcgt
     2941 ctccgttcag gaccgcaaag tggaattcga tctcgggcct gttcgcgccc ctcgcgattg
     3001 tctggcccac cagattgatg tatatcatga tctgggtgtt ctcgtacaac ccgtaccggc
     3061 cgatgatccg cgccgccacc gctcgtccga acctgatctc ctcgactatg tccgaggtcg
     3121 ccacgacacc cgcctcggtc gatatcctgt cgcgccaatt ctcttccggc tgggctgccg
     3181 ggatcgatga cgggcaaaga acggagagca tcaagagaac aactgtcatg gtgacggccg
     3241 gtttaatcat tctttgccct ccatgaactg tatgatctcc gagtaactca tcgtgaacaa
     3301 ctcgatctct tgaagggatt catagtcgac acgatcgccc ttgcgttccg atttctcgac
     3361 atccgtgtca agaccgcggt cagcttcgat gccggttgcg gacgcggccc gccgccggaa
     3421 gcccctccgt agttcctgct cgtctgccgt gatggcccac acgctctctt tcggcggatg
     3481 catcagcagc acgaaggaat ggacatagcc gtccccgttg ttgtacttga cctttgtcca
     3541 tccgccttcc ttccccgatg aaatgaattt gtatcctttg tcaacctcgc ccaggaccgc
     3601 ggacttgaaa gtggggccgg acatcacctt tgccttcacg ctctgaacat aatactcgtc
     3661 cgcggcatgc agggcgggcg catgccagag cgctacaacg atgaatacgg tgatcacgat
     3721 cgtctttgcc ataggtgaca ccttccgaac gggccttcac cgccggcttg acgttccggc
     3781 cggcataaac cctgatcgcg ctcgaatctc tgttgaattc tactcgaaac caattccact
     3841 gtaaaggggg tagtgctgct gcttgaaaaa cgaaagttac gccggttcac cggcagggaa
     3901 gaattccccg ggtaagaccg tttcatc
//
LOCUS       DASSLD010000453         5307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_948444,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000453 DASSLD010000000
VERSION     DASSLD010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948444"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFK23_08170"
     CDS             complement(<1..192)
                     /locus_tag="VFK23_08170"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HET7319094.1"
                     /translation="MKEAVMERDALEKGITLFNEGKYFEAHEAWEDLWLNERDASEKR
                     FYQGLIMAAGAMLHYTKREC"
     regulatory      complement(274..352)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(535..978)
                     /locus_tag="VFK23_08175"
     CDS             complement(535..978)
                     /locus_tag="VFK23_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319095.1"
                     /translation="MKRQLAERFNQEVENHRKALLYYARKCDWETFQAKAGRLFDYLE
                     SIEFRELERRFYSVFSLILGFLVLAVIGLMTVDFQVHQELPRLKHAFVMSAIAVSSFE
                     LYFYVDYRIYVDVKTRCYRKRREIFIREIERDFRGFAVQQERNAA"
     gene            complement(1021..1368)
                     /locus_tag="VFK23_08180"
     CDS             complement(1021..1368)
                     /locus_tag="VFK23_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319096.1"
                     /translation="MDDYLNSKRLCHVCREAMTTIHAEVAPGQHIFVCEKCLETAKQN
                     FIWVCMHCGNVFIRPKSLVVKRLRDPQLKRAYAECEDLQIIQGIDRCIECGPEEIMEY
                     VAAAKGEKNGGHA"
     gene            complement(2001..3272)
                     /locus_tag="VFK23_08185"
     CDS             complement(2001..3272)
                     /locus_tag="VFK23_08185"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET7319097.1"
                     /translation="MQRDHDRLHRDEQDLRSFGMTELQNIYADLGLSQDELDLLSVPR
                     QSFTVSFPVRLTAGKTQIFTGYRVLYNDARGPAKGGIRFHPEMDANHASELAFLMAIK
                     CAVVNIPFGGAKGGVAVNPIELSRRDLELVTRAYIRAIADYIGPFKDIPAPDVYTDEQ
                     IMAWILDEYERIRREHVPAVVTGKPSELGGIVARRYSTALGGTHVLEQALKEQGVEPR
                     GLRVAIQGFGNVGENTARILYERGYCVLAVSDVNGGILDPDGLDIPAVRAYKGKTGSV
                     TSFPGARSITNTELLTVDCDVLVPAALSNQIDANNANNVKAKIILELANAPTTSAADD
                     ILFERKVLLIPDILANAGGVVVSYFEWSQNLSNDTWPEEKVLSRLREIMITAYNDVSG
                     HCHEERCRMRKAAYELAVKRILNAERLRGNL"
     gene            complement(3380..4804)
                     /locus_tag="VFK23_08190"
     CDS             complement(3380..4804)
                     /locus_tag="VFK23_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HET7319098.1"
                     /translation="MDNVCAPGYSCSPSYSITIRVEIENRVGMFAKIATAISSAGGDL
                     GSVDIVRVEKGKIIRDVTVNAQNVVHEKAIVKAIKTVAGVKVLRVSDRTFSAHEGGKI
                     EIRNKLPVRDRNDLSKVYTPGVARVCMDIHEHREHAYRYTIKGNSVAVISDGTAVLGL
                     GDIGPEAALPVMEGKAMIFKEFAGIDAFPIVLATKNTDEIVETVKNISPAFGGINLED
                     FSAPHCFEVESRLRKLLDIPVFHDDQHGTSVVALAALINVSRLLKRDLKKFKVVVVGA
                     GAAGSAVARILLASGVKDIVACDRAGALYSGRKHGMDPYKRQLAKITNPRGVKGSISD
                     AMKGADVFIGLSAPNVITADDVRRMARDPVVFALANPDPEISPEEAHSLVRVMATGRS
                     DYPNQINNMLGFPGIFRGLLDVRARGVNEEVKLAAAHAIAHTVAKEELTEDYVIPSIF
                     DRTVVQSVSAAVAEAAKKTGLVKH"
     gene            5061..>5307
                     /gene="aspA"
                     /locus_tag="VFK23_08195"
     CDS             5061..>5307
                     /gene="aspA"
                     /locus_tag="VFK23_08195"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081847.1"
                     /note="catalyzes the formation of fumarate from aspartate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HET7319099.1"
                     /translation="MPDYRIEKDSLGEVRVPSDALYGAQTQRAVDNFPVSGIRFPRVF
                     IRSLGLIKAFAAEVNAGLGLLDPLIAQAIHQASLEVAD"
BASE COUNT         1083 a         1552 c         1414 g         1258 t
ORIGIN      
        1 gcattcccgc ttcgtataat gaagcatcgc cccggcggcc atgatgagcc cctggtaaaa
       61 ccttttttcc gatgcatcac gctcgttaag ccagagatcc tcccatgcct catgggcctc
      121 gaagtattta ccctcattga acagagtaat tcccttttcc agagcgtctc tttccatgac
      181 ggcttccttc ataataaccg ctcctcccat ttcctcgacc acccggcctg ccgcaggctc
      241 aaaaaaaagg ccgagcgctc gattgtggca tccttcggca gctcggccat cttcctcgtg
      301 aagattcgtg actttccgtc ccctcctcgc ggagggtttg gctttgtcgg gactctattt
      361 gatgttaggc ctgatcactt ttcagtatcc cgccttcgcg caaaatcttt ttgtgcggcg
      421 gtaaacgctc tgatacagga ttgtattcct ccttatttat ttatagctcc tgggcgcaat
      481 acttgtcaag aagcgaaagt tcgccaccgc gccggccggc aagttaaacc atagttacgc
      541 cgcgttccgc tcctgctgga cggcgaatcc ccggaagtcc cgttcgatct cccggatgaa
      601 tatttcccgg cgtttccggt aacagcgggt cttaacgtcg acatatatgc ggtagtcgac
      661 gtagaaataa agttcgaagc tgcttaccgc aatggcggac atcacaaagg cgtgcttgag
      721 cctcggcagc tcctggtgta cctgaaaatc caccgtcatg agaccgatga ccgcaaggac
      781 caggaaaccg agtatcaagc tgaagaccga gtagaatctc cgctccagct ccctgaactc
      841 tatggactcc agatagtcga acagcctccc ggccttggcc tggaacgtct cccagtcgca
      901 tttcctggca taatagagaa gcgccttccg gtggttttcc acctcctggt tgaaacgttc
      961 ggcaagttgc ctcttcatac gacatcaccc gccttttcct aataattcgc tataggcaca
     1021 tcaagcgtgc ccgccgtttt tttctccttt tgccgctgcg acgtactcca tgatctcttc
     1081 agggccgcat tcaatgcagc ggtctatccc ctgaatgatc tggagatcct cacactccgc
     1141 gtaggcccgc ttgagctgcg gatcgcggag ccgtttgact accagggatt tgggccgtat
     1201 gaagacgtta ccgcagtgca tgcagaccca gatgaagttc tgttttgccg tttccaggca
     1261 tttctcgcac acgaagatgt gctgccccgg agcgacttcg gcatggatcg tcgtcattgc
     1321 ctctctgcaa acatgacaga gccttttgct gttcagatag tcgtccataa tccccccttt
     1381 tgggtacggg aaaccctatt accgcgtgtc aacatggttg tatgccctgc gattccccga
     1441 ctgatcatgt cccgcttcca tcggcctcgt gtggaatgat ggatgaggcc ctgccgctcg
     1501 gtataagtct ttcttatact aaaataacaa taagcaataa acatgcccgc atgtatattg
     1561 caacttattg aagtcataag aaaaatattt tcagatagat tctacgtatt ttcaataata
     1621 tatacagcaa cgtaattagg tggaaaccac agatagggag gcagatccag gaaagcgtgt
     1681 caaaatggat gagtcaaatt gtgacaagga aggttaatat tgttacatta ctgacatagt
     1741 aaaaaccgat gagagaggga ctatgcctag caggttgctg aaaaactctc gtcaggtcat
     1801 tgcgaggagc gtttttcgca agcctgcccc agcatgatta aagtaggggc ggcaatctcg
     1861 aagttgttga tttcgttaaa aaagcgagat tgcggagtta atcccgagcc ataaaacgag
     1921 attgcttcgt tgcgatcaca ataacggtga tgacccgctc gcaaagacgg ccaaaagact
     1981 ttttcagtga cctcccagac ttaaagattc cctcggagtc tttccgcgtt caggatcctt
     2041 ttgaccgcca gttcgtaggc cgcctttctc atccggcacc gctcttcatg gcagtggcct
     2101 gagacatcgt tgtacgcggt gatcattatt tcccggaggc gtgacaggac cttctcctcg
     2161 ggccacgtat cattgctcag gttctggctc cactcgaagt agctcacaac gacgcctccc
     2221 gcgttcgcga ggatgtccgg gatgagcagg accttccttt cgaagaggat gtcgtccgcg
     2281 gccgaggtcg tgggtgcgtt ggcgagctcc agaatgatct ttgccttcac gttgttcgcg
     2341 ttgttcgcat cgatctggtt ggaaagcgcc gcggggacga ggacgtcgca gtcgaccgtc
     2401 agaagctcgg tattggttat ggaccgagcg ccggggaagc tcgtcacgct gcccgttttc
     2461 cccttatatg cccgcacggc gggaatatcg aggccgtcgg gatcaaggat gccgccgttc
     2521 acgtcgctca cggcgaggac acagtaaccc cgttcgtaca ggatccgggc ggtgttctct
     2581 cccacgttgc cgaatccctg tatcgccacc cgcagtcctc gcggttccac accctgctcc
     2641 ttgagggcct gttcgagcac atgagtcccg cccagggcag tggaataccg ccgtgcgacg
     2701 attcctccca gctccgacgg cttaccggtg accacagcgg gcacatgctc acgcctgatc
     2761 cgctcgtact cgtccaggat ccaagccatg atctgctcgt ccgtgtacac gtccggcgcc
     2821 gggatatcct tgaacgggcc gatatagtcc gcaatagcgc ggatgtaggc gcgcgtcacg
     2881 agttcgaggt cccgccggga aagttcgatc ggattgaccg ccacgccgcc ttttgccccc
     2941 ccgaagggaa tattcacgac cgcgcacttg atggccatga ggaaggcgag ttcgcttgcg
     3001 tggttcgcgt ccatctcagg atggaagcgg atgccgccct tcgccggtcc ccgcgcatcg
     3061 ttgtacagga cgcggtagcc agtgaagatc tgcgtcttac ccgcagtcaa tctgaccggg
     3121 aaactgacgg tgaaggactg ccgtggaacc gacagcaagt caagctcgtc ctgcgagagg
     3181 ccgaggtccg catagatgtt ctggagctcc gtcatgccga agctgcgcag gtcctgttcg
     3241 tcgcggtgta gccggtcatg gtcgcgctgc attttcacct cgataattca gttgggagtt
     3301 ttgagttcgg agttcggaga aaatgccctt gctttatgct ttcactaccg actccgaact
     3361 cctaactccg agctatgcac taatgtttca ccagtcccgt cttcttcgcc gcctcagcga
     3421 ccgcggcgga aacggactgt acgaccgtcc ggtcgaagat gctcgggatg acatagtcct
     3481 cggtcagttc ctcctttgcg accgtatggg cgatggcatg agcggccgcc agctttactt
     3541 cctcgttcac gccgcgcgcc cgcacatcca gaaggccccg gaagatgccg gggaagccga
     3601 gcatgttgtt gatctggttc ggatagtcgg acctccccgt ggccatgacc cgtaccaacg
     3661 agtgcgcctc ctcgggagag atttcggggt caggattggc aagggcgaac acgacggggt
     3721 ccctggccat cctgcgcacg tcgtcggcgg tgattacgtt cggcgcggaa agtccgatga
     3781 acacgtcagc gcccttcatc gcgtcagaga tgcttccctt caccccgcgg ggatttgtta
     3841 tctttgccaa ttgccgcttg taggggtcca tgccgtgctt ccgcccgctg tagagggcgc
     3901 cggccctgtc gcaggcgacg atatctttga cgcctgatgc caacagtatc ctggccacgg
     3961 cagaacccgc tgctcccgcg cccaccacga caaccttgaa ttttttgagg tcccgtttca
     4021 gaaggcgcga gacgttgatg agcgcggcca gcgcgacaac cgacgtccca tgctggtcgt
     4081 cgtgaaacac gggtatgtcg agcagcttcc tgagcctgct ttcgacctcg aagcagtgag
     4141 gcgcagagaa gtcttcgaga ttgataccgc cgaacgcggg agagatgttc ttgaccgtct
     4201 caacgatctc atccgtattt ttggtcgcga gcacgatcgg gaacgcgtcg atgccggcga
     4261 attccttgaa gatcatcgcc ttgccttcca tgaccggaag cgccgcttcg ggcccgatgt
     4321 cgccgaggcc caggaccgca gtgccgtcgg agatgacggc cacggaattg cccttgatgg
     4381 tgtagcggta ggcgtgctct ctatgctcat ggatgtccat gcagacccgc gccacgcccg
     4441 gcgtatagac tttggacagg tcgttccggt ccctcaccgg tagcttgttc ctgatctcga
     4501 tcttgccgcc ctcgtgggcg gagaaggtgc ggtccgacac cctgaggacc ttgactcccg
     4561 ccacggtctt gatggccttt acgatggcct tttcatgcac gacgttctgg gcgttgacgg
     4621 tcacgtcccg gatgatcttg cccttctcaa cgcgcacgat atccacggaa cccaggtcgc
     4681 cgccggcgct cgatatggcg gtcgcgatct tcgcgaacat tccgacgcgg ttctcgatct
     4741 ccaccctgat ggtgatgctg tagctgggac tgcaactgta tccgggagcg catacattgt
     4801 ccatggcaaa tctcctttac cacttattat agtttcaatt gcaactatgg tcaacttgcg
     4861 gtcttcagaa ttgcacacat gcgcggttgc gcaagaatgc cgctggacgt gtttttttac
     4921 aaaatcaccg tgcaaaaaga ggacaaaatg tgtataattg tgacattcgt tggcattaca
     4981 atcggcgaaa cgttttttag cctttgccca tttcttctat cgactccaga ctaacggctg
     5041 ttgaatcacg actgattctc atgcccgact accgtatcga aaaagactca ctcggcgaag
     5101 taagggtgcc gtctgatgcg ctctacggag cccagacgca gcgcgccgta gataatttcc
     5161 cggtgagcgg catccgcttc ccacgcgtct tcatccgcag ccttggcttg attaaggctt
     5221 tcgccgcgga agtaaacgca gggctggggc tgctcgatcc gctcattgcc caggctatac
     5281 accaggcgtc cctcgaggtt gccgacg
//
LOCUS       DASSLD010000454         2573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1463143, whole genome shotgun sequence.
ACCESSION   DASSLD010000454 DASSLD010000000
VERSION     DASSLD010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463143"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /gene="trpB"
                     /locus_tag="VFK23_08200"
     CDS             <1..881
                     /gene="trpB"
                     /locus_tag="VFK23_08200"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET7319100.1"
                     /translation="KTRLVAETGAGQHGVATATAAAMFGLDCEVYMGTDDVARQSLNV
                     FRMKLLGSTVRPVDIGSKTLKDAINEAMRDWITNVANTHYVLGTVFGPHPFPVMVRDF
                     QSVIGREAKKQIMKFEGRLPDALIACVGGGSNSMGLFHEFIDEPAVKMFGIEAGGLGI
                     EPGRHAARFAGGSLGVLQGSKTFVLQDKDGQIELTHSVSAGLDYAAVGPEHAFYHDSG
                     RIEYTYAVDEEAMEAFDLLSRLEGIIPALESAHAVAYCLKLAPTLGKDKVVIVNLSGR
                     GDKDVMQVAKMKGVEL"
     gene            934..1746
                     /gene="trpA"
                     /locus_tag="VFK23_08205"
     CDS             934..1746
                     /gene="trpA"
                     /locus_tag="VFK23_08205"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET7319101.1"
                     /translation="MSRIKNTFNKLRKKNETALIPYLMAGDPDLATTRSLIVEMEKAG
                     ADIIELGAPFSDPLADGPTIRKAAIRSLLHHTSIADVLGLVADVRKASPIPLIIMTYY
                     HLIFHYGEERFVNDAAAAGLDGVILPDLPPEEAGVLIPAAKKAGLDTIFLLAPTSTEE
                     RIKLVCKVSQGFVYYVSLTGVTGARAGVQSSVEESLRKIKTHTDKPVAVGFGISTPDQ
                     AAQIARWGADGVIVGSALVKVMEEYGSSPELIGKTAAFAKELKQGVLAGHAA"
     gene            1790..2557
                     /locus_tag="VFK23_08210"
     CDS             1790..2557
                     /locus_tag="VFK23_08210"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HET7319102.1"
                     /translation="MKVRILGCSGSESRGHMPPGFLVNDVMMLDAGTITAALGTRAQS
                     KITDILISHTHLDHIKSLLFLADNLVGRVRKPVVIRSIPKVLDAIRKHLMNDIIWPDF
                     TKIPTPGNAVLAYGPMPLGKAVTIAGLRVKAIPMNHPVPAVGFVVDDGASSILYSADT
                     GPNEGLWKEAARTRNLSAIIVDTSFPNSLDGIASVSGHFTPAQLNDDLTRAGVGNNVP
                     IYIYHIKPVHERKVLRDLAGLGRKNVKVLKEGKTYNF"
BASE COUNT          554 a          770 c          799 g          450 t
ORIGIN      
        1 gcaagacccg tctggtcgca gagacggggg ccggccagca cggcgtggcc acggccacgg
       61 cggcggccat gttcggactc gactgcgagg tttacatggg caccgacgac gtggcccgcc
      121 agtcattgaa cgtcttccgc atgaaactcc tgggctctac cgtacgtcct gtggacatcg
      181 ggagcaagac gctcaaggat gccatcaatg aggccatgcg ggactggata acgaacgtgg
      241 cgaacacgca ttacgtgctg ggcacggtct tcggccctca tccgttcccg gtgatggtgc
      301 gcgatttcca gtccgtcatc ggccgcgagg cgaaaaagca gatcatgaag ttcgagggca
      361 ggctgcccga cgccctgatc gcctgtgtgg gaggcgggtc gaactcaatg gggctgttcc
      421 atgagtttat cgacgagccc gccgtcaaga tgttcggcat cgaggcggga ggcctcggca
      481 tcgagccggg caggcacgcg gcgcggtttg ccggaggctc actcggcgtg ctccagggct
      541 ccaagacgtt cgtcctgcag gacaaggacg gccagatcga acttacccat tcggtgtccg
      601 caggcctcga ctatgccgcc gtcgggccgg agcacgcctt ctatcacgat tccggccgca
      661 tcgagtacac ctacgccgtg gatgaagagg cgatggaggc cttcgacctc ctgtcccgcc
      721 tcgaaggaat catccctgcc ctggagagcg cgcacgcggt cgcctactgc ctcaaactgg
      781 cgcccacgct ggggaaggac aaggtcgtca tcgtgaacct ctcgggccgc ggggacaagg
      841 acgtgatgca ggtggcgaag atgaagggcg tggagctgta acgaaagcgg aattcggaat
      901 gtggaataaa atcaaaatca ggacacctcg tacatgtcga gaataaaaaa cacctttaac
      961 aagctcagga aaaagaacga aacggcgctc atcccctatc tcatggcggg cgatccggac
     1021 ctcgcgacga ccagatcgct catcgtcgag atggaaaagg caggcgccga cattatcgaa
     1081 ctcggcgcgc ccttttccga tcccctggcg gacgggccca cgatccggaa ggcggccatc
     1141 cggtctctcc tgcaccatac gtcgatcgcc gacgtgctcg gccttgtcgc cgatgtgcgg
     1201 aaggcgagcc cgatcccgct catcataatg acctactacc acctgatctt ccattatggg
     1261 gaggagcggt ttgtgaacga tgccgctgcc gccggactgg acggtgtgat cctgcccgac
     1321 cttccgccgg aagaggccgg cgtcctgatc cccgcggcga agaaggcggg gctcgatacg
     1381 atcttcctcc tggcgcccac gagcaccgag gagcggatca agctcgtctg caaggtcagc
     1441 caggggttcg tttactatgt ctcgctcacg ggcgtgaccg gcgcgagggc cggggttcag
     1501 agttccgtcg aggagtccct gagaaagatc aagacccata cggacaaacc ggtcgccgtg
     1561 ggattcggaa tctccacgcc ggaccaggcg gcccagatcg cccggtgggg agcggacggc
     1621 gttatcgtcg gcagcgcact cgtcaaggtt atggaagagt acggaagctc cccggagctt
     1681 atcgggaaga ctgccgcttt tgccaaggag ctcaaacagg gcgtgcttgc gggacacgcc
     1741 gcctgacgag gacacgctat aataatagca tcggcaaaaa gggagacgga tgaaggtcag
     1801 gatactgggt tgttcgggtt ccgagtcgag aggccatatg cctcccggtt tcctcgtgaa
     1861 tgacgtcatg atgctcgacg cagggacgat caccgcggcg ctcggcacca gagcgcagag
     1921 caaaataacg gacatcctga tcagccatac tcacctcgat cacatcaaat cgctcttgtt
     1981 cctggcggac aacctcgtcg gccgggtgag gaaacccgtg gtcatccggt ccatccccaa
     2041 ggtcctcgac gcgatccgga aacacctgat gaacgacatc atctggcctg atttcacgaa
     2101 gatcccgaca ccggggaacg ccgtgctggc ctatgggccg atgccgctcg gaaaggcggt
     2161 gaccatcgcc ggcctccggg tcaaggcgat tcccatgaac catccggtcc ccgccgtggg
     2221 tttcgtggtg gatgacggcg cgtcatccat cctgtacagt gcagacaccg gtccgaacga
     2281 aggtctctgg aaggaggcag caaggacgcg gaacctctcg gccataatcg tcgatacgtc
     2341 gtttccgaac agcctcgacg gcatcgccag cgtgagcggt catttcacgc cggcgcagct
     2401 caacgacgac cttacaaggg ccggtgtcgg gaacaatgtc cccatttaca tctatcacat
     2461 caagccggtc cacgaaagga aagtgctcag agacctcgcc gggcttggcc ggaagaacgt
     2521 gaaggtcctg aaggagggga agacgtataa cttctgaaaa aagtttatac tta
//
LOCUS       DASSLD010000455         6401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_426906,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000455 DASSLD010000000
VERSION     DASSLD010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426906"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFK23_08215"
     CDS             complement(<1..477)
                     /locus_tag="VFK23_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319103.1"
                     /translation="MYETRAGEFLPTGPAEANDKTHGDIQFSTEKPVFEVAKFWWNSL
                     IPTAPRDSINLLVGITQLPLAYSPGKVRLSANQYPVYERRALDLISPKLLSSDLDDGL
                     FRLSEPQIILEVNGMVVPGKAVTDVGKRDTLWLEYHIGVTNGSNDKADNNNQKDFYG"
     gene            complement(556..915)
                     /locus_tag="VFK23_08220"
     CDS             complement(556..915)
                     /locus_tag="VFK23_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319104.1"
                     /translation="MKSFARTAVVITVLTATSALLLTPSAWAVPSYSRRYGVECSTCH
                     TMWGALNGAGVTFRLSGYRAINGRDLTPVDKDIELSNGLLVIPTTLPLSIITGVGIDA
                     RTEKREASDGTSMTRTG"
     gene            complement(1128..1778)
                     /locus_tag="VFK23_08225"
     CDS             complement(1128..1778)
                     /locus_tag="VFK23_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7319105.1"
                     /translation="MKKILIVDDDAEFRSHLIEVLREEGYETDSVSSAKDALAKNQTV
                     TYDVVLLDLMMPGMSGSDALMEIRKRHPRTKVIMITAFATVDNAVDAIRRGASDYISK
                     PFKIQELLTTIRRVLEEAKFEEGMKKLNFEQVLRSVASPIRRKILKLLHAGGRLRLME
                     ITRELGIEDHTKVVFHLRILKEAGSVEQDEEKSYLLTSEGVKTLDCLKTMEQTLLP"
     gene            complement(1756..3204)
                     /locus_tag="VFK23_08230"
     CDS             complement(1756..3204)
                     /locus_tag="VFK23_08230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7319106.1"
                     /translation="MFSLFAHITPVYILYLIYGAFFLFLGVSIATKEMKGSELKLANS
                     LWLLGVFGFLHGFHEWMKLGQLIEGKNLSARQIISGEGISVVLLVLSFLFLLQFGISL
                     INGLDTKRVRWVKGIPVPLLLIWVLFVWHYGFHKEGFHLDLDFLRQADIGARFTFGLV
                     GGMLTAYSLIAYSREVRVLSPSAAKKLFYTGITFIFYAVFTSSYPVPMLPIPIELLRG
                     ASAFLIACFIVKALNIFDVETRKKIERQMRRLVQAEKLTSLGQLAAGIAHEINNPLTN
                     ASLGIQTLKSRLRTNASGEDVVEKLDAVERNIDRASSIAQELLQFSRQQETEFVPLNI
                     DTVIQGALTLMQYKLSMVAIRKELEPVPEIMGDPGKLQQVFINVLANSVEAMPDGGTI
                     SLATSVQEEMVIVRIKDTGIGIEAENLSRVFDPFFSTKEIGSGTGLGLFICYGILKQH
                     GGTIEVASAIGRGTEVTVKLPVRKLHEKDTDR"
     gene            complement(3328..4389)
                     /locus_tag="VFK23_08235"
     CDS             complement(3328..4389)
                     /locus_tag="VFK23_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HET7319107.1"
                     /translation="MQSYYQYRDGKRLPDPAMRERIKILRIGLMLLLVIEGACPSMGW
                     AAESPKSGTLSVVLENDFFYGVDRDYTNGLMLIWVPGRDAPTPEWAMTLARLVPWFPE
                     QGEVRHGYAFGQSIFTPSDITIANPPLRDRPYAGWLYGTIELDVVSGRQRDQFGMTFG
                     MIGPASLAGRTQKFVHRVFPGNDPQGWYTQLGNEPGIVATYVRSWQGIATLTLSEARV
                     DFTPHIGAALGNVFTYGNAGVTMRYGKRLPSDFGPPRVQPGLPGSWDFSPVPGFGWYI
                     FAGIEGRAVARNIFLDGNTFRNSRSVDKKPLVGDLQFGMVLDLSDVRLSYTHVFRTRE
                     FQTQGSEDNFGSICLSVKF"
     gene            4739..5872
                     /locus_tag="VFK23_08240"
     CDS             4739..5872
                     /locus_tag="VFK23_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HET7319108.1"
                     /translation="MIINAKLTFDHTDGDTLVVRIAGEWTVGSPLPPAGMVEKELSSG
                     SGVRRVAFDATALGGWDSGLLTFLLKLFNRCSAAGVAVEKTGLPPGVVRLLDLATAVP
                     ERKGTRRESKRDPFLARVGDAAIRAWKANRDAVTFLGEVTVAALRMFRGKARFRRSDL
                     LETIQECGAQALPIVSLISLLVGLILAFVGAIQLQMFGAQIYVADLVGIAMVREMAAI
                     MTGIIVTGRTGAAFAAKLGTMQVNEEIDALKTMGFSPIEFLVLPRVVALFLMMPLLCL
                     YADVMGVLGGMIVGVGMLDLGIMQYYLQTRNAVHLNHFWIGLFSSAVFGILVAVAGCM
                     RGMQCGRSASAVGEATTSAVVTGIVSIIVATAVITLICNVLGI"
     gene            5898..>6401
                     /locus_tag="VFK23_08245"
     CDS             5898..>6401
                     /locus_tag="VFK23_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7319109.1"
                     /translation="MSGTREPCIVVKDLTMAFGSFVLQRDLNFSVDRGDIFIIMGGSG
                     CGKSTLLRHLVGLQMPAKGEVLYGETSFWDAETAERERIMRRFGILYQGGALWSSMTL
                     AENVALPLEEYTDLDPESIRERVSLKLSLVGLAGFEDYYPSEISGGMKKRAGLARAMA
                     LDPDILFF"
BASE COUNT         1318 a         1834 c         1867 g         1382 t
ORIGIN      
        1 cccgtagaag tctttctggt tgttgttgtc tgccttatcg ttgctgccgt tcgtcacgcc
       61 gatatggtat tccagccaca acgtatcccg cttaccgaca tcggtgactg ccttgcccgg
      121 aacgaccatg ccgttgacct ctagaatgat ctgcggttcg ctcagtctga acaggccatc
      181 atccaaatca gagctgagga gcttcgggct gatcaggtcc agcgcgcggc gctcgtatac
      241 ggggtactgg ttggcggaga gccgcacctt gcccggcgaa taggccagcg gaagttgggt
      301 aatgcctaca aggagattga tgctgtcgcg tggcgcggtc ggtatgaggc tgttccacca
      361 gaacttcgcc acctcgaaga ccggtttttc ggtgctaaac tggatatctc cgtgcgtttt
      421 gtcattggct tcggccggac ctgtcggaag gaactccccg gccctggttt catacatcgg
      481 gaactcgatg aaggcggaca ggttcttgcc gaccggcgac gtgaggaaga tgctggcgtc
      541 ctcgagagcg aggctctacc ctgtccgagt catgctggtt ccgtctgacg cctcccgttt
      601 ttccgtacgg gcatcgatgc cgacgccggt gatgatcgac aacggtaacg tcgtcggaat
      661 gacgagtaag ccgttcgaaa gctcgatgtc cttatcgacc ggtgttaggt cgcggccgtt
      721 gattgcgcga tatccgctga gccggaacgt gacccctgcg ccgttcaacg cgccccacat
      781 ggtatggcag gtggaacatt ccacaccata tctgcgcgag tacgacggca ccgcccacgc
      841 gctcggcgtg agcagcagcg cggatgttgc cgtcagaacg gtaatgacca cagcggttcg
      901 tgcaaagctt ttcatgattc ctcctccccg tgaaatatga cggatttcct tgtgcacatt
      961 aaattcgaat gccccccgaa acaatagaaa acatgttcac ttttcagcac tttctgtgaa
     1021 atggaattca caagcggatt cggccgcaac cccgtgaagg gcaagagcgg ggagtcggga
     1081 aacagttcca gaggaggaag aagtgaggga acagagggaa aacggcgtca ggggagcaga
     1141 gtttgttcca tcgtcttgag acagtccaag gttttcaccc cttcactggt gaggagatag
     1201 gatttttctt cgtcctgctc gacgcttccg gcttctttca gaatcctcaa gtgaaaaacg
     1261 accttagtat ggtcttcgat ccctaactca cgtgtgattt ccatcaagcg caggcggcct
     1321 ccggcgtgga gcaacttgag gatctttctc cggataggac tcgcaacaga tctcaatacc
     1381 tgctcgaaat taagcttctt cattccttct tcgaattttg cttcttcgag cacccggcgg
     1441 atcgtcgtca ggagttcttg gatcttgaag ggtttggaga tgtagtcgct ggcccctctc
     1501 cggatggcat cgacggcgtt atcgaccgtc gcgaaggccg tgatcatgat cactttcgtc
     1561 ctggggtgcc gtttccgaat ctccataagc gcatcgctgc cgctcattcc gggcatcatg
     1621 aggtccagga gcacgacgtc atacgtcacc gtctgattct tggcaagtgc gtccttggcc
     1681 gatgaaacgc tgtcagtttc atacccttcc tctcgcagaa cctcaatgag gtgggatcgg
     1741 aactcggcgt catcgtcaac gatcagtatc tttttcatgg agcttcctga ctggcaactt
     1801 aacggtcact tcggtacccc tgccgatcgc actggcaacc tcgatagtcc cgccatgctg
     1861 cttgaggata ccatagcaga tgaaaagccc cagaccggtg ccgctaccga tctccttcgt
     1921 tgaaaaaaag gggtcgaata cccgggaaag attttccgcc tcgataccga tccccgtatc
     1981 cttgatcctc acaattacca tctcctcctg cacggaagtg gcaagggaaa tcgttccgcc
     2041 atcaggcatg gcctcaacgg agttggcaag gacattgatg aatacctgtt ggagtttccc
     2101 cggatcgccc atgatctccg ggacgggttc cagttccttc ctgatggcta ccatgctcaa
     2161 tttatattgc atcagcgtga gcgccccctg gatcaccgtg tcgatgttca gggggacgaa
     2221 ttcggtttcc tgttggcgtg aaaactggag caactcctgg gcaatcgacg atgctcggtc
     2281 tatattccgc tccaccgcgt cgagcttttc cacgacatct tcgccggacg cgttcgtcct
     2341 caacctgctt ttcagcgtct ggattcccag agatgcatta gttaggggat tatttatttc
     2401 atgagcgatc cccgccgcaa gctgccctag ggaagtaagc ttttcagcct ggaccagacg
     2461 cctcatctgc cgctcgattt tcttcctcgt ttcaacgtca aagatattga gcgcttttac
     2521 aatgaagcag gcgataagaa atgccgatgc gccccggagc agttcgatgg ggattgggag
     2581 catcggcacg ggatagctac ttgtgaacac ggcgtaaaag atgaatgtga tcccggtgta
     2641 gaagagtttc ttcgcggcgg aagggcttaa aactcttact tcgcgcgagt acgctatcag
     2701 gctgtaggcg gtcagcatcc ctccgacgag tccgaatgtg aatctcgccc cgatatctgc
     2761 ctgtcgcagg aaatccagat caagatgaaa cccctcctta tgaaacccgt aatgccagac
     2821 gaagagaacc caaatcagga gaagaggcac agggattccc ttgacccacc gtacccgttt
     2881 tgtgtcgagt ccgttaatca gcgagatgcc gaattgtaat aggaagagga aagagaggac
     2941 gagcagaacg accgatattc cctctcccga aataatctgc cgggcagaca ggtttttccc
     3001 ttcgatcaat tgcccgagtt tcatccattc atgaaacccg tgaagaaatc cgaacacacc
     3061 cagcagccac aggctgttcg cgagcttaag ctcgctcccc ttcatttcct ttgtggcgat
     3121 ggacactccc aagaacagga aaaaggcgcc gtagataagg tacaggatat aaaccggcgt
     3181 aatatgggca aagagactga acatgtccga tcccgttcgt tccgtttctg tatatgatcc
     3241 cccacactca ttaggcggtt cacttctaag cagctggccg cacgttcgag tcgtgccagg
     3301 cgcgccacct tttccggata ctcgcggtta aaacttgacc gataggcaga tagaaccgaa
     3361 attgtcttcg cttccctggg tttggaactc acgagtacga aaaacatggg tgtagctgag
     3421 gcgcacatcg gacaaatcca ggaccatacc gaactgcagg tcgccgacca ggggtttctt
     3481 gtccacgctg cggctgttac ggaaggtgtt gccgtcgaga aagatattgc gcgcaaccgc
     3541 ccgtccctcg atgcctgcaa atatatacca gccaaaacca ggcaccggtg agaaatccca
     3601 ggagcccggc aagccaggct ggacgcgggg tggcccgaag tcgctgggca accgtttgcc
     3661 gtagcgcatg gttacacccg cattgccgta ggtgaacaca ttgccgagcg ccgcaccgat
     3721 gtggggagtg aaatccaccc gggcctcgga cagggttaat gtggcgattc cctgccaact
     3781 gcgcacgtag gtcgctacaa tgcccggctc attaccgagc tgtgtgtacc agccttgtgg
     3841 atcgttgcca gggaaaactc tgtggacgaa tttctgggtc cgccccgcca aggaggccgg
     3901 accgatcata ccgaaggtca tcccgaactg atcgcgttgc cgtcccgaca ccacgtccag
     3961 ttcgatcgtt ccatacagcc agccggcata ggggcggtcc cgcaatggcg gattcgctat
     4021 ggtaatgtcg ctcggcgtaa aaatgctttg gccgaaggca tagccatggc gaacctcgcc
     4081 ttgttcgggg aaccacggca cgaggcgcgc caacgtcatg gcccactcgg gtgtgggtgc
     4141 atccctaccc ggtacccaga tcaacatcag cccattggta tagtcccgat caacgccgta
     4201 aaaaaagtcg ttttccagaa cgacgctcag cgtcccggac ttcggtgatt ccgcagccca
     4261 acccatggat gggcaggcac cctcgatcac aagcagtaac atcaatccga tacgaagtat
     4321 ctttatacgc tcgcgcatcg ccggatccgg caaccttttg ccatcacgat actggtaata
     4381 ggattgcacg gacactccca tcttccgact ggggaggaaa atgcgaggtc ctcttcatgc
     4441 gtaccttctc cgtgggtgcc ggcatttcct gttttctggt ctatcggcgt caacccttct
     4501 cccccgggac cgggtcgatg agctcatcac aggtttctcg ttgtagatta tagccaatat
     4561 cgattactat cgatacagca gcgtaagctt gctcccggct ggacgaaggt ccacctcttc
     4621 cgcgagaccg cgcaacacat cgcgccaccg gaaaaggagc ttccaatggt tatttggtgc
     4681 ccggttccac ggaattcata ccaattgcgg accgggattc ccatggatga cctgccccgt
     4741 gattattaat gcgaagctga cgttcgacca caccgacggg gacaccctgg tcgtccgtat
     4801 cgcgggggag tggactgtcg ggagtcccct cccgccggcc gggatggtcg agaaggagct
     4861 ctcctccgga tccggagtcc gcagggtcgc attcgacgct accgcgctgg gaggatggga
     4921 cagcggcctt ctgaccttcc tcctcaagtt gttcaaccgg tgctccgccg cgggggtcgc
     4981 ggtggagaaa acgggccttc ctccgggggt cgttcggctc ctcgacctgg ccacggcggt
     5041 tcccgagcgg aaggggaccc gacgggagtc gaaacgggat ccgttcctcg cacgggtcgg
     5101 cgacgccgcc atacgggcct ggaaagccaa ccgcgacgcg gtgacattcc tcggcgaagt
     5161 gaccgtagct gcacttcgga tgttccgggg aaaagcccgg ttccgacgct ccgacctgct
     5221 ggagaccatc caggagtgcg gggcgcaagc cctccccatt gtctccctga tcagcctgct
     5281 ggtggggctg atcctcgcct tcgtgggggc catccagctc cagatgttcg gcgcccagat
     5341 ctacgtggcg gacctggtgg ggattgcgat ggtgcgggag atggcggcga tcatgaccgg
     5401 aatcatcgtg acggggcgca ccggggcggc gttcgccgcg aagctgggga cgatgcaggt
     5461 gaacgaggag atcgacgccc tgaagacgat ggggttttcc ccgatcgagt tcctcgttct
     5521 cccgcgggtg gtcgccctgt tcctgatgat gccgctcctg tgcctttacg cggacgtgat
     5581 gggggtgctc ggggggatga tcgtcggggt cgggatgctc gacctcggga tcatgcagta
     5641 ctacctgcag acacggaacg cggtgcattt gaaccacttc tggatcgggc tcttcagcag
     5701 cgcggtgttc gggatcctgg tggccgtcgc cggctgcatg cgcgggatgc agtgcgggcg
     5761 cagcgcctcg gcggtgggag aggcgacgac gtcggccgtg gtgaccggga tcgtcagcat
     5821 catcgtggcc accgctgtga tcaccttgat ctgcaacgtc ctgggaatct gacgggcaac
     5881 gggggaacgg ccgggagatg agcggaacga gggaaccgtg catcgtcgtg aaggacctga
     5941 cgatggcgtt cggcagcttt gtcctccagc gggacctgaa cttctcggtc gaccgcgggg
     6001 acatcttcat catcatgggc ggaagcggat gcgggaagag caccctgctt cgccacctcg
     6061 tggggctcca gatgccggca aagggggagg tcctctacgg agagacgagc ttctgggacg
     6121 cggagactgc ggagcgggag cggatcatgc ggcgcttcgg aatcctctac cagggggggg
     6181 cgctgtggag ttccatgacg ctggcggaga acgtcgctct gcctctggag gagtacacgg
     6241 acctggaccc ggaaagcatc cgcgaacggg tgtcactgaa gctgtcgctg gtcggcctcg
     6301 cggggttcga ggattattac ccctccgaga tcagcggggg aatgaagaag cgcgcgggcc
     6361 ttgcccgcgc catggcgctg gatcccgata tcctgttctt c
//
LOCUS       DASSLD010000456         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1576335, whole genome shotgun sequence.
ACCESSION   DASSLD010000456 DASSLD010000000
VERSION     DASSLD010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576335"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /gene="lpdA"
                     /locus_tag="VFK23_08250"
     CDS             <1..1290
                     /gene="lpdA"
                     /locus_tag="VFK23_08250"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET7319110.1"
                     /translation="GTCLNQGCIPTKTIIASAEALERARNAGEFGVEVSGAVGYSLEK
                     IRERREKVVSTQVKGIRGLFKSWGVGLIEGRGSLLSPDVVRVVQRDGTTMDVKSDRVI
                     VATGSRPAVLPGLAFDGESVMSSDDAVLLKSIPKSLLIIGAGVIGSEFAFIYRTFGAE
                     VTMVEMLPQAIATEDDEMADLIEREFKKAKIRLITNVKVDGLTRTDGGLLSARLSNGQ
                     EVGAEAVLVSVGRALNSGGLGLEEVGVKTGSRGEILVNEKMETSVAGIYAVGDITAKN
                     MLAHVASHQGLVAVDNALGGDERMDYSVIPSGIFTMPEIGSVGLREKEAAGKGVKYRV
                     GRFLYRALGKAHAMGEITGMVKIIADEATDKVLGVHVCGAHATDLVHEGALAMQTGAT
                     AAQLGRMIHAHPTLAEAVMEAASDVHKEAIHVPKVAK"
     gene            1287..>2078
                     /locus_tag="VFK23_08255"
     CDS             1287..>2078
                     /locus_tag="VFK23_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319111.1"
                     /translation="MRGRGAFRRFRLPAFVLALYWIGCMAAGAAHSAEIDVHGTVASF
                     VWKEFIDSERLLKESGVLTGVGVSFRDEYPDRLILDGSADIFGGRVGYAGGTQTGVPT
                     TSTVDYFGLNLKGDVGKSFPLAPTISMEPFAGLGFRTWLRHIHNGITPAGQPVQGYTE
                     QWMTLYARLGARSDFDISQGKRVFAAAGVKLPLYNENTAYLSREGIGPDVTMHPGWDW
                     SVFAEAGLKMDLVKVPVFYDGLRFARSPDVAVRQGLFFYQPRSTAD"
BASE COUNT          394 a          601 c          725 g          358 t
ORIGIN      
        1 ggaacctgcc tgaaccaggg ctgcatcccg acgaaaacca tcatagcctc ggcagaggcg
       61 cttgagcgcg cgcgaaacgc cggtgaattc ggtgtcgagg tgtccggcgc cgtcggctac
      121 agtctcgaga agatccgcga acgcagggag aaggtcgttt caacgcaggt taaggggatc
      181 cggggcctgt tcaagagctg gggggtgggc ctcatcgagg gccgcggatc actgctttcg
      241 cccgacgtgg tgcgtgtggt ccagagggac ggcacgacca tggacgtgaa atcggacagg
      301 gtgatcgtcg caaccggctc ccggcccgcg gtcctgcccg gcctcgcctt cgacggcgag
      361 agcgtcatgt cgagcgacga tgcagtcctg ctgaagagca tccccaaaag cctgctcatc
      421 atcggcgcgg gggtgatcgg ctccgagttc gccttcatct accggacgtt cggggccgag
      481 gtgacgatgg tcgagatgct gccgcaggcc attgccaccg aggacgatga gatggcggac
      541 ctgatcgaac gggagttcaa gaaggcgaag atcaggctca tcacgaacgt caaggtggac
      601 ggtttgacaa ggacggacgg cggtcttctc tctgcccggc tgagcaacgg gcaggaggtc
      661 ggtgccgagg cggtcctcgt gtcagtcggc cgcgccctca actcgggagg cctcggtctc
      721 gaagaggtcg gcgtgaagac cggcagccgc ggcgagatcc tcgtgaatga aaagatggag
      781 acgagcgtcg cgggcatcta tgccgtcggc gacattaccg cgaagaacat gctggcccac
      841 gtggcgtcgc atcagggcct cgtggccgtg gataacgcgc tcggcggcga tgaacgcatg
      901 gactacagcg tcatcccgtc gggtatcttc accatgcccg agatcggcag cgtggggctc
      961 cgggagaaag aggcggcggg gaagggtgtc aagtaccggg tcggacggtt cctctaccgc
     1021 gccctcggca aggcgcatgc catgggagag atcaccggca tggtgaagat catcgccgac
     1081 gaggcgaccg acaaggtgct gggcgtccat gtctgcggcg cccatgccac cgacctggtc
     1141 catgaaggcg ccctcgccat gcagacaggc gcgacggcgg cgcagctcgg ccgcatgatc
     1201 cacgcccacc cgacccttgc cgaggcggtg atggaagcgg cgtccgacgt tcataaggaa
     1261 gcgatccacg tgccgaaggt ggccaaatga gagggcgtgg cgcattcagg cggtttcggc
     1321 tgccggcatt cgtgcttgcg ctgtactgga tcggatgcat ggccgcgggt gcggcgcact
     1381 ccgcggaaat cgacgtccat ggaacggtgg catccttcgt ctggaaggaa ttcatcgaca
     1441 gcgaacgact gttgaaagaa tcgggcgtgc tcacgggggt cggcgtctcc tttcgggatg
     1501 aatatcccga tcggctgatc ctggacgggt cggccgatat tttcggcggc agggtgggat
     1561 acgcaggggg cacgcagacg ggggtcccga ccacgagcac ggtggattac ttcggcctga
     1621 atctcaaggg ggatgtgggg aaatccttcc ccctggcccc aaccatctcc atggagccct
     1681 tcgccggcct cggtttccgg acctggctac gccatattca taatgggata acgcctgcag
     1741 gacagccggt gcaaggatat acggagcagt ggatgaccct gtacgcccgg ctgggggcga
     1801 ggagcgactt tgatatctcc caggggaaaa gagtattcgc cgcggccggc gtgaagctcc
     1861 ccctgtataa cgagaacacc gcgtacctga gcagagaggg aataggcccg gatgtaacga
     1921 tgcatcccgg atgggattgg tcggtctttg cagaggcggg tctgaagatg gacctcgtga
     1981 aggttcccgt gttctacgac gggctgcgct tcgcccggtc ccctgacgtg gccgtgcggc
     2041 agggcctttt cttttatcag ccgcggtcca ccgcggac
//
LOCUS       DASSLD010000457         3534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_434099,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000457 DASSLD010000000
VERSION     DASSLD010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434099"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..804
                     /locus_tag="VFK23_08260"
     CDS             103..804
                     /locus_tag="VFK23_08260"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET7319112.1"
                     /translation="MKPKTWFQSANLAIDGIIYSVKTQRHMRYHLYAALAALVLSLVL
                     NISRLEFILLCMAIVIVLATETMNTAIETTVDMISAEFHPKAKLAKDIAAGVVLIASI
                     GALMLAYLILYPALRNTIAGRQWRLAKAPNDVIAFVSLAVVIILVIILKAIVGKGEPL
                     RGGMPSGHAAISFSIWCASLFLSDSLSVVVLTFLLASMVSWNRWSSGIHKPIEVVAGA
                     LLGACVTLLFFLVFS"
     gene            856..1209
                     /locus_tag="VFK23_08265"
     CDS             856..1209
                     /locus_tag="VFK23_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET7319113.1"
                     /translation="MLDQILEEVLSSVKGSEAVIFLDGEGETIAHAGGMAEDIKLLGA
                     WKEIHLDHIRESSRRLGIGDVRAVFFSLDGGNELLVPVSGEYCLLVFLSASAGVQGVM
                     AKLKTTVERLKREVE"
     gene            complement(1215..2177)
                     /locus_tag="VFK23_08270"
     CDS             complement(1215..2177)
                     /locus_tag="VFK23_08270"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HET7319114.1"
                     /translation="MLPQEYLEFEKPIAELEKKIEELTLFTSNGGIDLEEEILKLQKK
                     SDQLLAEIYSRLTPWQKVQISRHANRPYTLDYIEAMMTDFVEMHGDRGFADDPAIVCG
                     LAKLEHIPVVIIGHQKGRTLKERVFRNFGQPNPEGYRKALRLMRFAEKFNKPLITFID
                     TQGAYPGIGAEERGQGESIARNLSVMSMLKIPIVAVVIGEGGSGGALALGVADRVLML
                     EHATYSVISPEGCAAILWNNGAKASEAAELLKLTAQDLFHMRVIDEVVEEPIGGAHRD
                     PRRASELLKDAIARNLAEIRGVTAAELIQLRYDKFRKLGMYDEI"
     gene            2500..2862
                     /locus_tag="VFK23_08275"
     CDS             2500..2862
                     /locus_tag="VFK23_08275"
                     /inference="COORDINATES: protein motif:HMM:NF016261.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HET7319115.1"
                     /translation="MTPVLDYIEINEGAKRVAVDEDGYLVNFDDWDESVARALAERTG
                     FGELTGDRIEILKFIREHYKKYNFFPILRAVCKYVNKPRDCMIDEFEVPLVAWKLAGL
                     PHPEEPVISLLEAGQTPT"
     gene            3058..3492
                     /locus_tag="VFK23_08280"
     CDS             3058..3492
                     /locus_tag="VFK23_08280"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HET7319116.1"
                     /translation="MIKKIVGYLPLFFILALSFAGCEGGNPKLSIEGMRAELSPVMHG
                     EGMVYLRIVNAGGKDTLTQVRISIPGAAANLHEMRGNFMVLAKKMTIPAKSTLELQPR
                     ASHIMLENMPRDIKEGQPLTLTLVFQKSGEIKVPLVLMKAQF"
BASE COUNT          758 a          965 c          983 g          828 t
ORIGIN      
        1 gattgtcacc cccctccggg agaatcatcc gagggtgctt tccccgcttt cttcacgcgg
       61 ggacgggcct tgcggaggag ctttggagaa aggcatcttt ccttgaaacc gaaaacatgg
      121 ttccagagcg ctaacctggc aatcgacggc atcatctatt ccgtcaagac ccagcggcac
      181 atgcgctatc atctctatgc cgcattggcg gccctggtcc tgagtctcgt cctgaacatc
      241 tctcgcctgg aattcatcct cctgtgcatg gccatcgtga tcgtgctggc gaccgaaacg
      301 atgaataccg ccatcgagac gacggtagat atgatctcag cggaatttca tccgaaggcc
      361 aagctggcca aggacatcgc tgccggcgtc gtgctcatcg cctccatcgg cgccctgatg
      421 ctcgcctacc tgatcctcta tcccgcactc agaaacacga ttgccggcag acaatggcgc
      481 ctcgcaaaag ccccgaacga cgtcattgcc ttcgtttccc tggcggtggt gatcatcctg
      541 gtcatcatcc tgaaggccat cgtgggcaag ggagagcccc tcagaggcgg catgccgagc
      601 ggtcatgcgg ccatctcctt ttcaatctgg tgcgcctcgc tattcctgtc cgattcgctt
      661 tccgtcgttg ttctgacttt tctgctcgcg tccatggtga gctggaacag gtggtcgtcg
      721 ggtatccaca agcccatcga ggtcgtcgcc ggcgcccttc tgggcgcgtg cgtgacgctg
      781 ctttttttcc tcgtattcag ttgagccggg ggggaacgaa cgcaaagctg acgaagcgga
      841 agtgaaaggg gaggcatgct cgatcagata ctggaagagg tgctctcatc ggtcaaagga
      901 tccgaggcgg tcatctttct cgacggggag ggtgaaacca tcgcccacgc aggcggcatg
      961 gcggaggata tcaagctgct cggcgcctgg aaagagatcc acctggacca tatccgtgaa
     1021 agttccaggc gcctggggat cggtgatgtt cgtgcggtgt tcttttcgct tgacggaggg
     1081 aacgaattgc tcgtgccggt gtcgggagag tactgcctgc tggtatttct ctccgcttcc
     1141 gcgggagtcc agggcgtcat ggcgaagttg aaaaccaccg tcgaacggct caaacgggaa
     1201 gtcgagtgac cccctcagat ctcgtcgtac attccgagct tcctgaattt gtcataccgg
     1261 agctggatca gttccgcggc cgtaacgccc ctgatctcgg cgaggttccg cgcaatggcg
     1321 tctttcagca gttcggacgc ccgccgcgga tcgcggtgag cgccgccgat cggctcttcc
     1381 acaacctcgt cgatcacccg catgtggaac agatcctgcg ccgtcagctt taaaagttct
     1441 gcggcttcgc tcgccttggc gccgttgttc cagaggatgg cggcgcaccc ttccggggaa
     1501 atgaccgaat aggtcgcatg ctcgagcatc agcacccggt cagcgacgcc cagagccagt
     1561 gcccccccgc tccccccctc gccgatcacg acggcgacga tcgggatctt gagcatggac
     1621 atgacgctga gattcctggc gattgattct ccctggccgc gttcttccgc gccgatgccg
     1681 gggtaggcgc cctgggtatc gatgaaggtg atgagcggct tgttgaactt ctcggcgaat
     1741 cgcatgagcc tgagcgcctt acgatacccc tcggggttcg gctggccgaa attgcggaag
     1801 actcgttctt tcagggtccg gcctttctgg tgtccgatga tgacgacggg aatgtgctcg
     1861 agctttgcca agccgcagac aatggcggga tcgtcggcga atccgcggtc gccgtgcatt
     1921 tcaacgaaat cagtcatcat ggcttcaata tagtccaacg tgtagggacg gttcgcatgt
     1981 cgcgatatct ggaccttctg ccacggcgtc agcctcgaat atatttcggc cagcaactga
     2041 tccgattttt tctgcagttt gagaatttct tcttcgaggt caatgccccc gtttgaggta
     2101 aatagcgtca gttcctcgat ctttttttca agttccgcta tcggtttttc gaattcgaga
     2161 tattcctgcg gcaaaatgag ctcctcgtgc cgttcctgtg tcaaaaacag atgtgccgcg
     2221 catggtgacg cggcaactgg gaagggtctg tattattgaa aatatattat ctgatattgg
     2281 cgtctccttg tcaagaactt gttcgcgcga ggaccgcggg tggtgtgaga aacgtgaaat
     2341 tttgcctttc atgcaggatt gttacgaaat tcccgttcaa gcgagggcac ctgtgggaaa
     2401 tacaagcgat ttaaagtagc tttagtcaac tcttgacacc cgccggtaac gcgttctaca
     2461 ataaaattag cagcaggaag aatccgagga ggattgacta tgacgcctgt ccttgattat
     2521 attgaaatca atgagggagc gaagcgggtt gctgttgatg aagacggcta cctcgtgaac
     2581 tttgacgact gggatgagtc cgtcgccagg gccttggcgg aacgcactgg ttttggcgaa
     2641 ctcaccggcg ataggatcga gatcctgaag ttcatacgtg aacactataa gaagtataac
     2701 ttctttccga ttcttcgcgc tgtgtgcaag tacgtcaaca agccgcggga ctgcatgatc
     2761 gatgaattcg aagtccccct ggttgcgtgg aaactggccg gtttacccca tcccgaggag
     2821 cccgtcatca gccttcttga ggcgggtcag acgcccactt aggcaaaccc tatcgaacaa
     2881 aggggcatgc acgaagggag ttccgccccg tttgaaagta aaaatatgat tttttttcaa
     2941 gtgtgcccgt cgttcgcttt actcttttag catgtctaat tatgtcttgc cttttccctc
     3001 ggcatatagt actttcgact catgcaacac ccacgattct cagcagggag gaaaaccgtg
     3061 ataaaaaaaa tcgttggata tcttccactc ttcttcatcc tggccttgag tttcgctggc
     3121 tgcgagggcg gcaatccgaa gctatccatt gaaggaatgc gcgccgaact ctctcccgta
     3181 atgcacggcg aaggaatggt gtatctcagg atcgtgaacg ccggagggaa agacactctg
     3241 acacaggtaa ggataagcat cccaggcgcg gcggcaaacc tccatgaaat gagaggaaac
     3301 ttcatggtcc tcgcaaaaaa gatgaccatt ccggcaaaga gcacgctcga gctgcagcct
     3361 agggcctccc acatcatgct cgaaaacatg cccagggata taaaggaagg ccagcctttg
     3421 actcttacgc tcgtattcca gaaatcagga gagataaagg ttccattggt gctcatgaag
     3481 gcgcagtttt gatgtgcggg caggaatatg agcatcaagc ccaggtaatg cgta
//
LOCUS       DASSLD010000458         2583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1146206, whole genome shotgun sequence.
ACCESSION   DASSLD010000458 DASSLD010000000
VERSION     DASSLD010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146206"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /gene="lysA"
                     /locus_tag="VFK23_08285"
     CDS             <1..881
                     /gene="lysA"
                     /locus_tag="VFK23_08285"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET7319117.1"
                     /translation="DELQALNEVAGEMGVKARIALRVNPDIDPKTHSYISTGLRENKF
                     GIPIEHALEHYLTARSLPNVEIVGVHHHIGSQITEVGPFVEALDKVAALVTELRAAGM
                     NIAYIDIGGGLGITYKDETPPQPQELASAIQGRIPRDCTLVLEPGRAIVGNAGVLVTS
                     ALYRKDSGAKHFLIVDAGMNDLIRPSLYEAYHEIKPVRESPAAERAVFDVVGPICESG
                     DFLAKDRELPVVRKGELLAVMSAGAYGFSMASNYNSRPRAAEVMVRGRDYFIVRERET
                     YNDLVKGEKVPGWLKG"
     gene            911..1723
                     /gene="dapF"
                     /locus_tag="VFK23_08290"
     CDS             911..1723
                     /gene="dapF"
                     /locus_tag="VFK23_08290"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET7319118.1"
                     /translation="MKKIPFHKMQGSGNDFILIDNRRAVLKGMDLRKLAMTVCHRHYS
                     VGADGLIVIVPSRKADFKWRFFNADGSEAEMCGNGSRCAARFAYLRKIAKKDMAFETL
                     AGVIRAQVKNDRVKVQLPGASGLRANIAVPLDSGLRMGHFINTGVPHLVYLTKDIENE
                     DVERIGRTTRYHELFKPAGTNVNFVQIQGRNRLRIRTYERGVESETLACGTGSVAAAL
                     VAGALGVVSPPVEVLTSGGERLIVSFDRIADGFDNIHLEGGAEVICEGTLYI"
     gene            1817..>2583
                     /gene="dapA"
                     /locus_tag="VFK23_08295"
     CDS             1817..>2583
                     /gene="dapA"
                     /locus_tag="VFK23_08295"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET7319119.1"
                     /translation="MFKGSMVAIVTPFKNGKVDEKALGDLIEFHIKNGTDVIVPCGTT
                     GESATLSHEEHHRVVEFTVKTVNKRIPVIAGAGSNSTSETMELTAYAKKAGANGVLLI
                     TPYYNKPTQEGLYRHFAKIADSTDIPIVLYNVPGRTSVNMLPPTVARLREKKNIVGIK
                     EATGDMKQVSEVIRLCGRDFDVISGDDFTTLPLLALGGVGAISVTANIAPADCAALFD
                     AWFAGKTDAALKLHYKLEPLHGPMFIETNPIPVKTSLA"
BASE COUNT          545 a          789 c          815 g          434 t
ORIGIN      
        1 gggacgaact ccaggccttg aacgaggtcg ccggagagat gggcgttaaa gcgaggatcg
       61 cgctcagggt caacccggac atcgatccca agacccactc ctacatctcc acggggctca
      121 gggagaacaa gttcggcatc ccgatcgagc atgcgctcga gcattacctg acggccaggt
      181 cgctgccgaa cgtcgagatc gtcggcgtgc accaccatat cggatcgcag atcactgaag
      241 tgggaccgtt cgtggaagcc ctcgataagg tcgccgcgct cgtgacggag ctccgcgccg
      301 cgggcatgaa tatcgcgtac atcgacatcg ggggcggcct cggcattacc tacaaggacg
      361 agaccccgcc ccagccgcag gaacttgcat cggccatcca ggggcgcatt ccccgcgact
      421 gcacgctcgt cctcgagccg ggccgggcca tcgtcggcaa cgcaggcgtc ctcgtgacga
      481 gcgccctgta ccgtaaggac agcggcgcaa agcacttcct gatcgtcgat gccggcatga
      541 acgatttgat ccgccccagc ctctacgagg cgtaccacga gatcaaaccc gtccgggagt
      601 cgccggccgc cgaacgcgca gtgttcgatg tggtcggacc gatctgcgaa tcgggcgatt
      661 ttctggcgaa ggaccgcgag ttgcccgtcg tcaggaaggg ggaacttctt gccgtcatga
      721 gcgccggggc ttatgggttt tccatggcct cgaactataa ttcgcgcccc cgggccgcgg
      781 aagtcatggt ccggggaaga gattacttta tcgttcgtga acgggaaacc tacaacgacc
      841 tggtgaaggg ggaaaaggtc ccgggctggc tgaagggatg aaccgagtgc ggaatgcgga
      901 atgaaagacg atgaagaaga ttcctttcca taagatgcag gggagcggga acgatttcat
      961 cctgatcgac aaccgcaggg ccgtcctcaa gggaatggac ttgaggaagc tggcaatgac
     1021 cgtctgccac cggcattact cggtgggcgc cgacggcctc atcgtgatcg tcccctcccg
     1081 gaaggccgac ttcaagtggc ggttcttcaa cgccgacggg agcgaggccg agatgtgcgg
     1141 caacggctcg cggtgcgccg cgcggttcgc ctacctcagg aagatcgcga agaaggacat
     1201 ggccttcgaa acgctagcgg gcgtcatccg cgcccaggtg aaaaacgacc gggtgaaggt
     1261 ccagctgccc ggcgcgtcgg gcctgcgcgc gaacatcgcg gtcccgctcg acagcggcct
     1321 tcgcatgggc cacttcataa acacgggcgt ccctcacctc gtctatctca cgaaggacat
     1381 cgagaacgag gacgtggagc ggatcgggag gaccacgcgg tatcacgagc tgttcaagcc
     1441 ggccgggacg aacgtgaact tcgtccagat ccagggccgg aacaggctca ggatcaggac
     1501 ctatgagcgc ggcgtggaga gcgagaccct ggcctgcggc acgggctccg ttgccgcggc
     1561 cctcgtcgcg ggggccctcg gcgttgtctc tccgccggtc gaggtgctga cctcgggcgg
     1621 ggagcggctc atcgtctcgt tcgaccgcat tgccgacggg ttcgacaaca tccacctcga
     1681 aggcggggcg gaagtcatct gcgaaggaac gctgtatatt tagtttcagg tctcaagtct
     1741 ccagtcccaa gttctcaacc tgagacttca aacttgagac tcgaaacacg atgtatgtcg
     1801 taataaggag gttgccatgt tcaaaggatc gatggttgcg attgtcacgc ccttcaaaaa
     1861 cggaaaggtc gatgaaaagg ccttgggcga tctgattgag tttcatatca agaacggcac
     1921 ggacgtcatc gtgccctgcg ggacgacggg cgaatccgcg acgctctcgc acgaggagca
     1981 ccaccgcgtc gtggagttca cggtgaagac ggtgaacaag cgcatacccg tcatagccgg
     2041 cgcgggctcg aactcgacgt ccgagacgat ggagctgacg gcgtacgcaa aaaaggcggg
     2101 ggccaacggc gtcctgctca tcacccccta ctacaacaag cccacgcagg aggggctgta
     2161 ccggcatttc gccaagatcg ccgattcgac ggacattccg atcgtcctct ataacgtgcc
     2221 gggccggacg agcgtcaaca tgcttccccc cacggtcgcg cgccttcggg aaaagaagaa
     2281 catcgtcggc atcaaggagg ccacgggaga catgaagcag gtgagcgagg tcatccggct
     2341 ctgcggcagg gacttcgacg tgatctccgg cgacgacttc accacgctgc cgctcctcgc
     2401 gctcggaggc gtgggcgcca tatcggtgac ggccaatatc gcgcccgcag actgcgctgc
     2461 cctgttcgac gcatggtttg ccggcaagac ggacgcggcg ctcaagctcc actacaaact
     2521 agagcccctg cacggcccca tgttcatcga aacgaacccg atcccggtca aaacgtcgct
     2581 tgc
//
LOCUS       DASSLD010000459         2130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1370657, whole genome shotgun sequence.
ACCESSION   DASSLD010000459 DASSLD010000000
VERSION     DASSLD010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370657"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VFK23_08300"
     CDS             <1..1044
                     /locus_tag="VFK23_08300"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HET7319120.1"
                     /translation="KSGSAPLKVRRELLSISISPGGATIMEGKTQQFSAIGNFSDKTT
                     ENLTSSARWQISDAKIAGINGNGLATGKTEAGTAAVTATFGERVGTGTITVTRIALAS
                     LAVIPAEATIRQMVSQQFTVQGTFSDGSTQDLTKSVAWTSSAPLIAPIKATGIASGVV
                     VGSSVITATCQGKSASSNLTVLSREIYRPRANEETAKKFVYWPAPRFSRVSGVQPTHG
                     AVVIDRLTGLLWTADATTPGPSACSPGGPLSWQAAVAYVACLNSNKYLGYRNWRLPSR
                     EEIFSILDYKQSVPNPLIYTQGFSYTQAFYGYDVWFLNSDGITSYADLSLGVSYYADG
                     SKPFYVWPVRSVK"
     gene            complement(1161..>2130)
                     /locus_tag="VFK23_08305"
     CDS             complement(1161..>2130)
                     /locus_tag="VFK23_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HET7319121.1"
                     /translation="NMVEWEGFRDPASGLPVYSLYGKVRKPTHSMLEHCDVVAVDLQD
                     MGSRYYTFIWTMDHCMQACQEAGKAVVVLDRPNPLGGRLVEGPVLDPAYASFVGLKPL
                     PVRHGMTIAEIGTYLHETFYPGLDFRIIPMRGWKRTMWFDDTKLPWIIPSPNMPTFDT
                     ALVYPGMCLFEGTNISEGRGTTRPFEIFGAPFIHSETIVKVLREFRLPGVSFRPHSFQ
                     PTFQKYAGILCGGAQVHVTDRGKFRPFKTGVAILKAIRNTWPHDFKWKEPPYEYEEVK
                     LPIDILAGTDRLRKDIEAGKDLLEMETWWKEEAKAFEKIRKKHLLY"
BASE COUNT          432 a          631 c          637 g          430 t
ORIGIN      
        1 aaaagcggtt ccgcaccgct caaggtcagg cgcgagcttc tttccatttc gatctcgccg
       61 gggggcgcga cgatcatgga gggcaagacc cagcagttca gcgccatcgg gaacttttcg
      121 gacaaaacaa cggagaacct cacgtcgtcg gcaagatggc aaatctccga tgcgaagatc
      181 gccggaatca acgggaacgg ccttgctacg ggcaagacgg aggcgggaac cgcggccgta
      241 acagccacct tcggcgaaag ggtcgggacg gggaccatta ccgtaaccag gatagcgctc
      301 gcatccttgg ccgtcattcc ggccgaggcg accatccggc agatggtatc acagcaattt
      361 accgtccagg gtacgttttc cgacggcagc acccaggatc tgacgaagtc cgttgcctgg
      421 acttcgagtg cgccgttgat agccccgatc aaggccacgg gaattgcaag cggcgtcgtg
      481 gtcggatcgt cagtgattac cgcgacctgc cagggcaagt cagcctcgtc gaatctcacg
      541 gtgctgtcgc gtgaaatata ccgtccccgg gctaatgagg agacggcaaa aaaattcgta
      601 tactggccgg ccccgaggtt ttccagggtc agcggagtgc aaccgacaca cggcgccgtg
      661 gtcatcgacc gcttgaccgg gctcctctgg acagcggacg ccaccacccc cgggccttca
      721 gcctgctccc ccggagggcc cctttcgtgg caggccgcgg ttgcctatgt ggcgtgcctc
      781 aacagcaaca aatatctcgg gtaccggaac tggcggcttc cgagccgcga ggagattttc
      841 agtatcctcg attacaagca atccgtcccg aatcccttga tatacacgca ggggttcagt
      901 tacacacagg ctttctacgg gtacgacgtc tggtttttaa acagcgacgg catcacgagc
      961 tatgccgatc tgagcctggg ggtttcgtac tacgccgacg ggtccaagcc cttctatgtg
     1021 tggcctgtcc gttccgtcaa ataggatgcc gtgttatctc cacccggtca ttgaatagca
     1081 gcaaagggga gcagagcgaa gagattgacc attcgctcaa tgctctttgc cgggtgacgg
     1141 gctatttacg cgcgccggca ttaatagagc aggtgcttct tccttatttt ctcaaacgcc
     1201 ttcgcttctt ccttccacca cgtctccatc tcgaggaggt ccttccctgc ctcgatgtcc
     1261 ttgcgcaggc ggtcggtgcc cgcgaggatg tcgatgggca gtttcacttc ctcgtactcg
     1321 taaggcggtt ccttccactt gaagtcatgg ggccaggtgt tgcggatcgc cttcaggatc
     1381 gccacgccgg tcttaaaggg cctgaacttc ccgcggtcgg tcacgtggac ctgggcgccg
     1441 ccgcacagta tgccggcata cttctggaac gtgggctgga acgaatgcgg acggaacgac
     1501 acccccggca gcctgaattc acggagcacc ttcacgatcg tctcggaatg gatgaacggc
     1561 gcgccgaaga tctcgaatgg cctggtggtg ccgcggcctt ccgatatgtt cgtcccttcg
     1621 aagaggcaca tgccgggata gaccagggca gtgtcgaagg tcggcatgtt gggtgagggt
     1681 atgatccacg gcagtttcgt atcatcgaac cacatggtcc gtttccatcc ccgcatgggt
     1741 atgatgcgga aatccaggcc ggggtagaaa gtctcatgaa gataggtccc gatctcggcg
     1801 atggtcatcc catgtcggac gggaaggggc tttaagccca cgaaggacgc gtatgccgga
     1861 tccagcaccg gcccttctac caagcgaccg ccgaggggat tcggccggtc gaggacgacg
     1921 actgccttgc ccgcctcctg gcacgcctgc atgcagtggt ccatggtcca gatgaaggtg
     1981 tagtacctgc tgcccatgtc ctgcagatcg accgcaacaa cgtcgcagtg ttcaagcatg
     2041 gaatgggtcg gttttcgcac cttgccatac aggctgtaaa caggaaggcc ggaggcgggg
     2101 tcacggaagc cttcccactc gaccatgttg
//
LOCUS       DASSLD010000460         2807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1535353, whole genome shotgun sequence.
ACCESSION   DASSLD010000460 DASSLD010000000
VERSION     DASSLD010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535353"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="VFK23_08310"
     CDS             <1..1278
                     /locus_tag="VFK23_08310"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7319122.1"
                     /translation="TNGSPILKNKISSGVASLDTLIDGFSVGDNVVWEVEAGTAYSVF
                     IRNFISRSYIDNQKVIYVSFNRSPQTVLNDLNTVMSSEHFTLIDCFTSGKGKNDNTFL
                     KFYEKPDPGVVRIEAPGDIDRFTATLNAIEDNLPPGARYVFDSLTGMQDLWGDENLTH
                     RFFTYMCPRLYDLGTVAYWFLEKEAHSPRFKANLRHITQVVFDLAKRKNSLFLKALKL
                     EGRQDRELFRPHQYDIRGDAVMVTSLKKEPATDIGSKLKEERIRLGISQKELADKVSL
                     TPSFLSQLENNQVSPSLGTYLQLCRALAINPGQFLEFDGGKTGTPWLLRKEGISSRPP
                     VREDAAKVYDVFSGSKVTARMVVLPQGAAMNRHFFYHKDEELVYIIGGDLSVTVNGKE
                     ERVSTGDVIHLQESFPTHWKNEGGDAAQILVVW"
     gene            1420..>2807
                     /locus_tag="VFK23_08315"
     CDS             1420..>2807
                     /locus_tag="VFK23_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HET7319123.1"
                     /translation="MNNGTPNASAATGTRNRVQDMAPQSGICSVCLDGCAGSCEVGKS
                     AYRGREVIYPMPFGKITAGGTKKYPVDYTHLNIQGTCVGAVGVAADSDKAIFPAVSTE
                     VEVGNKHKVKLSLPVFTGALGSTDIAKENWEGLAIGAAISGIMVVIGENVVGMDPKAE
                     IKNGAVMSSPELERRVKCFQEWYNGAGEIILQQNVEDTRLRSAEYAIEKLGVNCIELK
                     WGQGAKNIGGEVKLKTLARAEELKRRGYIVLPDPEDKGVRRAFAEEEFKEFERHSRLG
                     MVEQEGFVKAVKHYRSAGAKFVSLKTGAYRVSDLARAIRYASDAEIDLLTIDGAGGGT
                     GMSPWRMMNEWGIPTIYLQSLAYKFASQLKAKGKYVPDMAIAGGFSLEDHVFKALALG
                     APFFKAVCMGRALMIPAFVGKNIKKWIEEDKLPVDIKKYGDVPERIFITTESLKSRYG
                     KEFARIPWGAVA"
BASE COUNT          659 a          721 c          857 g          570 t
ORIGIN      
        1 acaaacggaa gtccgatact aaagaataaa atatcctcag gtgtggccag cctcgatacg
       61 ctgatcgatg gtttttccgt gggcgacaat gtcgtctggg aagtagaggc tgggaccgca
      121 tacagcgtct tcatccggaa cttcatctcc cgatcataca tcgacaacca gaaggtgatc
      181 tatgtgagct tcaatcggtc gccgcagacc gtgttgaacg atctcaatac ggtcatgtct
      241 tccgagcact ttacgctcat agactgtttc acctctggca aggggaagaa cgacaatacg
      301 ttccttaaat tctatgaaaa gccggatccc ggcgtggtgc ggatcgaggc gcccggtgac
      361 atcgaccggt tcacggcaac gctcaacgcc atcgaggaca atctgcctcc gggagcgcgc
      421 tacgtgtttg acagcctcac gggcatgcag gacctctggg gcgacgagaa tctcacccac
      481 cggttcttca cctacatgtg cccgcggctg tacgatttgg gtacggtagc gtactggttc
      541 ctggaaaagg aggcccacag cccgcgcttc aaggcgaacc tgcggcatat cacgcaggtt
      601 gtgttcgacc tcgcgaagcg gaagaacagc ctcttcctga aggccctgaa gctggagggc
      661 agacaggacc gggaattgtt cagacctcat cagtacgaca tccgcggcga cgctgtgatg
      721 gtaacgtcgc tcaagaaaga gcctgccacg gatatcggca gcaagttgaa agaggagcgt
      781 atccgactcg gcataagcca gaaggagctt gccgacaagg tcagtctgac gccgagtttt
      841 ttgtcgcagc tcgagaacaa ccaggtgagc ccctcgctcg gcacttatct ccagctgtgc
      901 cgggccctgg ccatcaatcc cggccagttc ctcgaatttg acggaggaaa gaccggtacg
      961 ccatggcttc tgcggaagga ggggatctca tcccgtcctc ccgtgcgcga ggacgctgca
     1021 aaggtgtacg atgtattttc ggggagcaag gttacagcac ggatggtggt gttgccacag
     1081 ggtgctgcca tgaaccggca ttttttttat cataaggacg aggaattggt ctatatcatt
     1141 ggcggcgatc tttctgtaac ggtcaacggt aaggaagagc gggtgagcac cggagatgta
     1201 atccaccttc aggaatcgtt ccccacgcat tggaagaacg aaggaggtga tgcagcgcag
     1261 attctcgttg tctggtagta tgaccatgtt gtgaacgcga tgttaaggca gaagcgtgcc
     1321 gaggaggggc attgcgagag cgcatttttt ttgaccaaca ggttaataaa tactgaatta
     1381 ttgatttaaa aaattactat tacctaacag gagggtaaca tgaataacgg aacaccaaat
     1441 gcaagtgcag caacaggaac tcggaacaga gtccaggaca tggcgccaca atcaggtatc
     1501 tgctcagtct gccttgacgg atgcgccggt tcctgcgaag taggcaagtc agcctaccgg
     1561 ggcagggaag ttatttaccc catgcccttt ggaaagatca ccgcaggggg aacgaagaag
     1621 taccccgtgg actacacgca cttgaacatc cagggcacat gtgtcggtgc tgtaggcgtt
     1681 gccgcagatt cggacaaggc catatttcct gcggtctcta ccgaggtgga agtcggcaac
     1741 aagcataaag tgaagctctc gctcccggtg tttaccggcg ccctgggctc caccgacatt
     1801 gcgaaggaga actgggaggg gcttgctatc ggtgcagcga tttcagggat catggtcgtg
     1861 attggcgaga acgtggtggg catggacccc aaagcggaga tcaagaacgg cgcggtgatg
     1921 agttcgccgg agctggagcg ccgcgtgaag tgcttccagg agtggtacaa tggcgcgggc
     1981 gaaattattc tccagcagaa cgtggaggac acgaggctcc gctctgccga gtatgccatc
     2041 gaaaagctcg gcgtgaactg catcgagctc aagtggggcc agggcgccaa gaacatcggc
     2101 ggtgaggtaa agctcaagac cctggcgcgg gcggaagagc tgaagcggcg tggttacatc
     2161 gtgctgcccg accccgagga caagggcgtg cgcagggcct ttgcagagga ggagttcaag
     2221 gaattcgaac gccactcgcg cctcggcatg gtcgagcagg agggttttgt gaaggctgtg
     2281 aagcattacc ggagcgcggg tgcaaagttc gtttcgctta agactggcgc ttaccgtgtg
     2341 tccgaccttg cgcgggccat ccgatacgcc tcggacgcgg agatcgacct gttgaccata
     2401 gacggcgctg gcggtggcac aggcatgagc ccttggcgta tgatgaatga gtggggcatc
     2461 ccgaccatat accttcagtc cctggcctac aagttcgcct cgcagctcaa ggcaaagggc
     2521 aaatatgttc ctgacatggc cattgccggc ggcttctccc tggaagacca cgtgttcaag
     2581 gctctggctc tgggcgctcc gttctttaag gcagtctgca tgggcagggc gctcatgatc
     2641 ccggcctttg taggcaagaa catcaagaag tggatcgagg aggacaagct gcccgtcgat
     2701 atcaaaaagt acggcgacgt gccggagcgg atcttcatca ccacagagtc gctcaaatcg
     2761 aggtacggca aggaatttgc gaggattccg tggggtgctg tggccat
//
LOCUS       DASSLD010000461         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_923745,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000461 DASSLD010000000
VERSION     DASSLD010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923745"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1347
                     /locus_tag="VFK23_08320"
     CDS             136..1347
                     /locus_tag="VFK23_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET7319124.1"
                     /translation="MDDRDFRELMSRVISQLTAAVTNTGLYTPEHPQVAQYMERAYAA
                     LSEMLGERPEVTIILIGNDLVADNRQLPSTGAVSFIDNFIRILKKKAIERLTFVAGVP
                     KPELQALIQDLASHDGAPIRSTAFIKLGKVELRVKTSGEQAGLADAPPLAGFSEGLTD
                     AQQEEMRKLTALTENELRELKELHLRIKRHKKIDVRGVDDMVKGFIRGFRQELNPLSL
                     LASLKSAHEYTFTHVTNVCILTMSLAESLGFKGEHLHRVGVASLLHDVGKLFIPEDIL
                     GKTGKLTDDERRQIETHSVKGARYLMGIEGIPKLAVLAALEHHLKYDGSGYPTIKGGW
                     QPNIVSQIITVADVFDAMRSKRSYQEAHSLGKIAGVLINGSGKAFNPVLVARFLKLIR
                     YQPPRTQAQGS"
     gene            complement(1385..>2665)
                     /locus_tag="VFK23_08325"
     CDS             complement(1385..>2665)
                     /locus_tag="VFK23_08325"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HET7319125.1"
                     /translation="RLLVRSVDALDSGSKREDPDLEGRVKLDAAGALWRVHSDLEGGW
                     DGTVRRPARDASLFKSWDRVYQSNTPYLEFKELYVTHSWGGLDIRAGIQRFAWGRLDE
                     YPPNDLLNPWDYTRFLEKPLEDRKIGVPSLSATLTRPDWAYEAVWVPVFIPYRLPLPD
                     ERWAGTSLVSAIAQAVPNAEIRPQEPDLPERRVENSSFGVRVRRTGEIEWALNLFHGY
                     DTRPVFRTTELAIIPGAGNVVIDPGYVPDFHRISVIGADVAAVRGDVSLRAEIALSFH
                     RFLNVQRPLWGYPASPAPGVYALNPIEQKRDTADYGVGADYRLFEDGLLTVQAQQTVI
                     LGNTDQFYEKKRETILWLNLKAGFLNQKIETNVNVALNPEHGDTMAKANAWYVLTDAW
                     KAGVTAMNLDGPPQSIFGRYSRNDQVEAEVVYSW"
BASE COUNT          563 a          787 c          787 g          528 t
ORIGIN      
        1 gcgcggtatc cacgggagag gctcacgaac ctcatccgga tcggagaagg gctcccgagc
       61 gacaagatca gaaggatctg cagaaaatat tcggaagaag cgtgatgccg cagcagggac
      121 cgcatcagga gcggaatgga cgatcgtgat tttcgggaat tgatgtcacg ggtgatttct
      181 cagcttactg ccgcagtgac gaatacgggc ctgtatacgc cggagcatcc ccaggtggcg
      241 cagtacatgg agagggcata cgccgcgctt tcggagatgc tcggtgagag gcccgaggtc
      301 acgattatcc tgatcggcaa cgatctggtt gcggataaca ggcagctgcc gtcaacaggg
      361 gctgtttcct ttatcgacaa ttttatccgg atcctgaaaa aaaaggcgat tgaacgcctc
      421 acctttgtcg cgggcgttcc gaagcccgaa ctccaggccc tcattcagga ccttgcctct
      481 cacgatggcg cgccgatccg atccacggcg ttcatcaagc tcggaaaagt ggagctcagg
      541 gtgaaaacgt ccggggagca ggcaggcctg gccgacgcac cgccccttgc cggcttctcg
      601 gaggggctga ccgatgccca gcaggaagag atgcggaagc tgacggcatt gacggaaaat
      661 gagctccgcg agctcaagga gctgcatctc agaatcaagc gccataagaa aatcgacgta
      721 cgcggcgtag atgacatggt gaaggggttc atcaggggtt tccgccagga gttgaaccct
      781 ttgagcctgc tggcgtcgct caagtccgcg cacgaataca cgttcaccca cgtcaccaac
      841 gtctgcatcc tgaccatgag cctggcggag tcgctgggat tcaagggaga gcaccttcac
      901 cgggtcggcg tggcttcttt gctccatgat gttgggaaac tttttatacc cgaggacatc
      961 ctgggaaaga ccggaaaact gaccgatgac gagcggcggc agatcgaaac acacagtgtc
     1021 aagggggccc gctacctgat gggaatcgag gggatcccga agctcgccgt actcgcggca
     1081 ctcgaacacc atttgaaata cgatggaagc gggtacccca cgatcaaggg gggctggcag
     1141 ccgaacatcg tgagccagat cataaccgtc gcggacgtgt tcgatgccat gcgaagcaaa
     1201 cgctcgtacc aggaggccca ttccctgggg aagatcgcgg gcgtgctcat caacggaagc
     1261 gggaaggcct ttaatcccgt cctggtcgcg cgctttttga agttgatacg gtatcagccg
     1321 ccgagaacac aggcccaggg ttcctagttc acgtgcacgg ttcgtaatcc ggtaccgcgg
     1381 cgccttacca cgaatagact acttccgctt ccacctggtc attcctggaa tacctcccga
     1441 agatcgactg cggagggccg tccagattca tcgcagtgac cccggccttc caggcatcgg
     1501 tcaaaacata ccatgcgttc gccttcgcca tggtgtctcc gtgctcggga ttgagggcca
     1561 cgttcacgtt cgtctcgatt ttctggttca ggaagcccgc cttgaggttc agccagagga
     1621 tggtctcccg ttttttctca tagaactgat ctgtattgcc gaggatgacc gtctgctggg
     1681 cctgcacggt caggaggccg tcttcgaaca gccggtaatc cgctccaacg ccgtaatccg
     1741 ccgtgtcgcg cttctgttcg atggggttga gagcgtagac ccccggcgca ggagacgccg
     1801 gataccccca cagggggcgc tgcacgttga ggaagcgatg gaagctcagg gcaatttctg
     1861 ccctgaggct cacgtctccc ctgaccgcag caacatcggc gccgatgacg ctgatccggt
     1921 gaaaatcggg aacgtatccg gggtcgatca cgacattccc cgctccgggg atgatcgcaa
     1981 gctccgttgt cctgaacacg ggacgcgtat cgtacccatg gaacaggttc aaggcccatt
     2041 ctatttctcc cgtgcgtctt acccggactc caaagctgct gttttcaact ctgcgctccg
     2101 gcaggtcagg ctcttgcggt cttatttcag cgttcgggac ggcctgcgcg atagccgaga
     2161 cgagcgacgt gcctgcccac cgctcatcgg gaaggggaag ccggtaaggg atgaagaccg
     2221 ggacccaaac ggcctcatag gcccagtccg gcctggtcag cgttgccgag agagaaggga
     2281 ccccgatctt cctgtcctcg agcggctttt cgagaaacct cgtgtaatcc caggggttca
     2341 gaaggtcgtt cggcggatac tcgtcgagcc ttccccatgc aaagcgctgg atgcccgccc
     2401 gaatgtcgag accgccccat gagtgcgtaa cgtaaagttc cttgaattcg agataaggcg
     2461 tattgctctg gtagacccgg tcccagctct taaaaagaga agcatccctc gccggcctcc
     2521 tgacggttcc gtcccagccg ccttcgagat ctgagtggac gcgccataac gctccggccg
     2581 catcgagctt cactctcccc tcaaggtccg gatcctctct tttgctgccg ctgtccagcg
     2641 catctacgct gcgcacgagc agtct
//
LOCUS       DASSLD010000462         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1175928, whole genome shotgun sequence.
ACCESSION   DASSLD010000462 DASSLD010000000
VERSION     DASSLD010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175928"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFK23_08330"
     CDS             <1..298
                     /locus_tag="VFK23_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HET7319126.1"
                     /translation="DLNTILRSLGMDDAFDSTIADFSGIDGLRDLYISGVVHQAFISV
                     DEKGTEAAAATAISVGVTSAPPPPLSLTIDHPFLFLLRDRQTGLILFMGKVVSL"
     gene            384..1943
                     /locus_tag="VFK23_08335"
     CDS             384..1943
                     /locus_tag="VFK23_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7319127.1"
                     /translation="MKQSDNKTVTVLLVEDDSNDARLIEYALSRVMKTPYQVVCAGRL
                     SQARECLCGREVDIILLDLGLPDGYGLTTFDQIHALTPLVPVIVLTGQDNDELAVEAV
                     RRGAQDFLVKGRFDSGLLYRSLHYAIERNRMLIELRQAQEALRASEMKYRKIFENVQD
                     VLYQADANGNLLDISPSITNHTGYAREELIGKSEASLYADPDAGSRLSQAIEEKGRVS
                     DYELIVRAKDGRLFPASLNARIVYDQAGRPAGREGVWRDISERKKMEEERLKHHVLES
                     IGILAGGIAHDFNNLLNVINGDIYIAKKLLQPEDKTYCRLTDAEQVCEIAGELSRRLI
                     TFATGGDPVRKTMQMSELLAGTIGGQLKGSAIKPEFDLPEDLRPVAIDEGQMKQVFAN
                     LVMNAKEAMPEGGTLTIRGENLTVASTDNLPMSEGEYVRISIGDTGVGIAPQNLAKVF
                     DPYFSTKNTYHERGLGLSLAVCYSVLKRHNGLLTVESEVGRGTTFHLYLPVAGRMSLR
                     EEGGQTWSEAA"
     gene            2213..>2663
                     /locus_tag="VFK23_08340"
     CDS             2213..>2663
                     /locus_tag="VFK23_08340"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET7319128.1"
                     /translation="MKHLRILFVFGFVLLFVTAASAAPKQSACVECHKKVTPGIVQQH
                     LDGKMGKKGVDCSACHGSAHMKMDDAKLASMPTPETCARYHPKQTEQFKAGKHNLAWI
                     ASSSMPMMGHQPKAVAGEGYKGCSSCHKIGAKPEAEKSQIRYGHAQCD"
BASE COUNT          621 a          765 c          769 g          508 t
ORIGIN      
        1 agatttgaac actatcctgc gttctctcgg tatggatgat gcctttgact caacgatcgc
       61 ggacttttcc ggcattgacg gactgcgtga tctttatatc agcggcgtcg tgcatcaggc
      121 attcatcagc gttgatgaga aagggacaga ggcggctgcc gccaccgcta tttcagtcgg
      181 cgttacctca gcgcctccgc ctccgttgtc gctgaccatc gaccatccct tcctcttcct
      241 gctccgcgac cgccagaccg ggctgattct gttcatgggg aaggtggttt cgctgtagca
      301 gaagtatggt ttgaaccgga tgcgaaattc cggagacggt gtcctcgagc tcaacgggaa
      361 gaagacgaag caaagattgt gagatgaaac agtcggacaa caagacagtc accgtgctcc
      421 tcgtagaaga cgattcgaac gacgcccgtc ttatcgaata tgccctctcc cgcgtcatga
      481 agacccccta ccaggtcgtc tgcgccgggc gcctgagcca ggccagggaa tgcctgtgcg
      541 gccgcgaggt cgacatcata ctgctggacc tcggactgcc ggacgggtac ggcctcacga
      601 ccttcgatca gatacacgca ttgacgccgc ttgtcccggt catcgttttg acggggcagg
      661 ataacgacga gctggccgtc gaggccgtgc gccggggtgc ccaggacttc ctggtaaaag
      721 gcaggttcga cagcggcctg ctgtaccgtt cgctccacta cgccatcgaa cggaaccgga
      781 tgctgatcga actcaggcag gcacaggaag cgcttcgggc gagcgagatg aaatacagga
      841 agatcttcga gaatgtccag gacgtgctct atcaggcgga cgcgaacggc aatcttctcg
      901 acatcagccc ttccatcaca aaccataccg ggtatgcgcg cgaagagctt atcggaaaat
      961 cggaggcaag cctgtacgcc gatcccgatg ccggctcccg gctctcacag gcgatcgagg
     1021 aaaaaggacg cgtttcggac tacgagctta tcgtgagggc gaaagacggc agattgttcc
     1081 ccgcgtcgct caatgcacgg atcgtgtatg atcaggcagg ccggcccgcg ggaagagaag
     1141 gagtatggag ggacatcagc gagcgcaaga agatggagga agaacgcctg aagcaccacg
     1201 tgctcgagtc aatcgggata ctcgcaggcg gcatagccca cgatttcaac aacctgttga
     1261 acgtcatcaa cggggacatt tacatcgcaa aaaagctgct gcaacccgaa gacaagacat
     1321 actgccgcct caccgacgcg gaacaagtct gcgaaatagc cggcgagctc agcaggagat
     1381 tgatcacctt tgcgaccggc ggcgatcccg tacgaaaaac catgcagatg tctgaattgc
     1441 tggccgggac gatcggcggt cagttgaaag gctcggccat aaagcctgaa ttcgatctgc
     1501 cggaagacct ccggcctgtc gcgatcgatg aaggacagat gaagcaggtt ttcgccaacc
     1561 tcgtcatgaa cgcaaaggag gcgatgcctg agggaggaac gctcacgatc cggggcgaga
     1621 accttacggt tgcctcgacg gacaacctgc cgatgtcgga aggtgaatac gtcaggatat
     1681 cgatcggcga tacgggagtc ggcatagccc cgcaaaatct ggcaaaggta ttcgatccct
     1741 atttttcgac taaaaacacc taccacgaga gggggctcgg actcagtctc gccgtctgct
     1801 attccgttct caagcgccac aacggcctgc ttacggtgga atcggaagtc ggcaggggaa
     1861 caaccttcca cctttacctc cctgttgccg gccgcatgtc gcttcgtgaa gaaggaggtc
     1921 agacatggag cgaggcggca tagccttctc tggttcgtga atgcgagcag ttgtctgacc
     1981 atcgatcacc ctttcctctt ctagctccgc aaccgccaga cctggctggt cctcttcatg
     2041 ggaaaggcgg tctcgctgta acgggatatg atctgcgtca ttcacatttt tcccgctttc
     2101 ccgtagagta tgccggtttg agcgaaaacg accgctctca atttcaggag gagctgcagc
     2161 ctgatcaact acgactgatt caaaccatct cacaacaagg aggtaccaca gcatgaagca
     2221 tctcaggatt ttgttcgtat tcggttttgt tctgctcttc gtcacggccg cgtcggccgc
     2281 gccgaaacag agcgcctgcg tcgagtgtca taagaaagtg acgcccggca tcgtccagca
     2341 gcacctcgac gggaagatgg ggaaaaaagg cgtcgactgc tcggcctgcc acggctcggc
     2401 ccacatgaag atggatgacg cgaagctcgc gtcgatgccg acgcccgaaa cctgcgcccg
     2461 ctaccatccg aagcagacgg aacagttcaa ggccggcaag cacaatctcg cgtggatagc
     2521 gtcgagttcc atgcccatga tgggccacca gccgaaagcg gtcgctggcg agggatacaa
     2581 aggctgctcg agctgccaca agatcggcgc caaacccgag gcggagaaat cgcagatccg
     2641 ctacggccat gcccagtgcg act
//
LOCUS       DASSLD010000463         6369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_915510,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000463 DASSLD010000000
VERSION     DASSLD010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915510"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFK23_08345"
     CDS             <1..181
                     /locus_tag="VFK23_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319129.1"
                     /translation="REESAMCYNCGCGKPGDDMGDPKNITDKTFEEAAKASKQSPKEA
                     KSKTLELLKIILKKE"
     gene            391..996
                     /locus_tag="VFK23_08350"
     CDS             391..996
                     /locus_tag="VFK23_08350"
                     /inference="COORDINATES: protein motif:HMM:NF018247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET7319130.1"
                     /translation="MGKIIAGKPYRIGVLFAVVVAAVLSLAAGSVLAAGALVDINTAD
                     QKTLESLPGVGPATAKEIVKGRPYKSVDDLANVKGIGKSKLEKIKPLVTVGGQPAAQA
                     PAAAGAAAPAASPKATTATQPAQKAPAQAKPAAKATVPTGPVNLNTASKEQLEALPGI
                     GPKKAQAIIDGRPYQKTEDIMKVKGIKQKTYDKLKDKITVQ"
     gene            complement(1335..1520)
                     /locus_tag="VFK23_08355"
     CDS             complement(1335..1520)
                     /locus_tag="VFK23_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HET7319131.1"
                     /translation="MPKVISCRDAGVDCDFVARGETEEELFRNAFEHGRKSHGMKEIP
                     KDLQEKMRKLIREEKAA"
     gene            complement(2050..3108)
                     /locus_tag="VFK23_08360"
     CDS             complement(2050..3108)
                     /locus_tag="VFK23_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET7319132.1"
                     /translation="MRNSTSIFLFVLGLLLCQVRVASAHHPSVGFGPGTAGPIVTIPA
                     ITMEKNRWSAGIRADYQKFDRFSDSNLESLAASGIETHSMDYQTSVFFSAGYGLTEDL
                     TLGLHIPYVIRRGIREGHLEDGIPEVHDHGDSGGIGDLSLLGQYRLFRMENAGADVSV
                     LFGLKTPTGVNKANDRDGVRFETEFQPGSGSWDPLIGAAAGRRLGPASLDASVLYAIA
                     TKGAQDTDLGDALHFGLSFSWRHTPGHGGIRASGEPHSHGTSASHAAEHAGDHRHLSL
                     DFILEAIGEWRQKQTIAGIRDPNSGGMTILVSPGLRANFFDRFAAFVSVGVPVIQDLN
                     GIQHETQYRVVFGISAGL"
     gene            complement(3348..4058)
                     /locus_tag="VFK23_08365"
     CDS             complement(3348..4058)
                     /locus_tag="VFK23_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319133.1"
                     /translation="MGRPCLSPFFLVTTAMTQIADIEVSRGYTKLASGVLLFLGILLF
                     LNYGVARLSDLLAFQMWPSDVDTAVFIVFGSAVLYVFLMALPFMPGIEIGLMLMAMLG
                     VGGIVLVYLCTVLALSLSFLAGRLLPPRYIARALGWMRLERARKLVTELEPLSAEERF
                     RFLARSTPSRIVPFLLHHRYLVLAVLFNLPGNVIIGDGGGIALIAGMSRLFPFSKYVL
                     LVCLAITPVPLLFLLRAV"
     gene            complement(4080..4715)
                     /locus_tag="VFK23_08370"
     CDS             complement(4080..4715)
                     /locus_tag="VFK23_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319134.1"
                     /translation="MADEFICDTSEVRRFIGAVKEIVSGPASVEARLAAIRPLFSQMM
                     ADPDWLPAEFRRTPEAGGMGKGIANWLLYRDTEGTLSLCALVLPHGAATPVHDHLAWG
                     LVGLYVGEQDEEIYEAATPVGPDDRHTDLTLVAKKHLSVGSFYDLIPPKGDIHRVVTT
                     SDEPSISLHLLGNDVGCVWRHRFEPETGEVAPFRSGYSNKECENDNRNPGS"
     gene            complement(4724..5197)
                     /locus_tag="VFK23_08375"
     CDS             complement(4724..5197)
                     /locus_tag="VFK23_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319135.1"
                     /translation="MIAPLSTTSLAAIAIALISVTATGADAIPGAIGATASPSAVPTV
                     SLLDGKTFVAQSGEKGKQASNKDTIIFRDGRFLSEGCSPFGFKDASYQATVDGAAIRF
                     HTETHSPTHGTMVWVGTVKDNAIEATSVWTRERWYWKIKKEYWYRGQPKNENRRE"
     gene            6140..>6369
                     /locus_tag="VFK23_08380"
     CDS             6140..>6369
                     /locus_tag="VFK23_08380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HET7319136.1"
                     /translation="MSFRRRITRRQFIKGAAIAGGVMMLPIKYIGSGTAYAFAQSPGL
                     KLWKTTLRGVGPGGIPVADKDGFTAPVTGVTH"
BASE COUNT         1466 a         1700 c         1887 g         1316 t
ORIGIN      
        1 aagggaggaa agtgccatgt gctacaactg cggatgcggg aaacccggcg acgacatggg
       61 ggatcccaag aacatcactg acaagacatt tgaggaagcg gcgaaggctt ccaaacagtc
      121 tccgaaagag gcaaaaagta aaaccttgga gttgttgaag atcatcctga aaaaagagta
      181 aacgcttggc cccgacggaa tacccggata tctggaaacg gaggaggtgg cgatattccc
      241 cacggaacag ttgaaagagg aacatgcgga agtcgagttg atattggaca tccttgcgaa
      301 agtttgcgtt cgcctcgaat cgggcaagcg cgtggactaa catacaacgc acagtatacc
      361 ggagtgcatt ccaacataaa ggagggtgag atggggaaga tcattgccgg aaaaccgtat
      421 cgcattgggg tgttgttcgc ggtcgtcgtt gcagcggtcc tttcactggc cgcggggagt
      481 gtcttggccg cgggcgctct ggtcgacatc aatacggcgg atcagaagac cctcgagtcg
      541 cttcccggcg tcggcccggc gacggcgaag gagatcgtca agggacggcc gtacaagagt
      601 gtagatgatc ttgcaaacgt aaaggggatc ggaaagtcga aattggaaaa gatcaagcct
      661 ctggtgaccg tcggggggca gccggccgcc caggcacccg cagccgcagg cgccgccgca
      721 ccggccgctt cgcccaaggc cacgaccgct actcagcctg cccaaaaagc tccggcgcag
      781 gcgaagccgg ctgcaaaggc cacggtaccc acgggccccg tcaacctcaa caccgcctcc
      841 aaggagcaac tggaggcgct gccggggatc ggcccgaaga aagcccaggc catcatcgat
      901 gggcggccgt accagaagac cgaggacatc atgaaggtga aggggatcaa gcagaagacc
      961 tacgacaagc tcaaggacaa gatcaccgtc cagtgaaatg aggaaaaatc cgaaagggtg
     1021 attccggggc ggggcgacaa tcatagaccg ccccgccttt tccgttatat cttcaggtct
     1081 ctcccccccc ggccagtggc tgtcgcgaat cgtggggaaa ataaagaggg aagaccatat
     1141 ggaacgcaaa ccccgtgcaa acaagtgcta ttttcagggt ctggaagggt gtttcgcaga
     1201 gacggtgttt acggggggaa tccggactat tccctgagtc agggtcgacg catcccgatt
     1261 cgattctgag agaagggccc gtgcagaatc ccgggccctc acaggatggt tggattatgt
     1321 tgactggacg tctcctacgc cgctttctcc tcccggatga gcttacgcat cttttcttgg
     1381 aggtcttttg gaatttcctt catgccgtgg gatttccttc cgtgctcaaa ggcgttgcga
     1441 aacagttcct cttccgtttc cccccgggcc acgaaatcac aatccacacc cgcgtcccga
     1501 caacttatga cttttggcat agagcacctc cccttccttc taaggtttca gttcaaaagt
     1561 ccccttggta ttaccattaa ggttcagttt caatagagga ggattcctcg atggagataa
     1621 tagaaaaggc catggaaaga aggggggcat ctatcgtgtg ggcggggacg gtgttgacgg
     1681 gtgaaacccc tccatgattg cagcgggaat tgggaccatt ttaatttaat tttcccggtt
     1741 tatttgaaat tgatgacggg atttccgtcc attcatccag aagatgacgg ggcgggacag
     1801 ttttattgat tcggtttcgg ttatagggga gtggacaact tggcggccca actctcttaa
     1861 tattgggggg agtggtgggg aaacccatgc gggtttaaat cccgccttcg gcaccatctc
     1921 aatacttaaa gggaattcga cctaggccag gttccctttt ttcgtaaaaa ttgctcaagt
     1981 gtccaaaaag tggcttgtgg atttcctggg tttacacgaa ggctaatcga acgacgtgac
     2041 gagaccggcc tacagccccg cactgattcc gaatactact ctgtattgag tctcgtgttg
     2101 gattccgttc aggtcctgaa tcaccggaac tccaactgag acaaacgcgg caaagcggtc
     2161 gaaaaagtta gctcgcaggc cgggggaaac caaaatcgtc atacctccac tgttgggatc
     2221 cctgattccc gcaatagtct gcttctggcg ccactcaccg attgcttcga ggatgaagtc
     2281 gagggagagg tgacggtgat ccccagcgtg ctcggcagcg tgcgatgcac tagtaccgtg
     2341 agagtggggt tctcccgatg cgcggatacc gccgtgacct ggggtatgac gccaggaaaa
     2401 agacagaccg aaatgaagcg cgtccccgag gtcggtgtcc tgcgcccctt ttgtggcgat
     2461 tgcgtaaagc acgcttgcgt ccaaggaggc cgggcccagg cgccgtcccg ccgccgctcc
     2521 gatcaacggg tcccaggagc cggatcctgg ttggaactcc gtttcgaacc ggacaccgtc
     2581 tctatcattc gccttgttaa ctccggtggg agttttcaag ccgaacagca cggatacgtc
     2641 ggcaccagcg ttctccatgc gaaaaagtcg atactgcccg aggagcgaga ggtcgccgat
     2701 tcctccggag tccccgtgat cgtggacctc gggtatgccg tcctccaggt gtccctcccg
     2761 aatccctcgc cggataacgt acggtatgtg tagtcccaac gtaaggtcct cggtcagccc
     2821 gtacccggcg gagaagaaga cactggtttg gtagtccatg gaatgggtct ctatgccgga
     2881 cgcggccagc gattcaagat tcgaatcgga aaaccggtcg aacttctggt aatccgctcg
     2941 tattccagcc gaccacctgt tcttctccat cgtgattgcc gggattgtca cgatgggtcc
     3001 cgccgtcccc ggaccgaatc ccacggaggg gtggtgcgcc gacgcaactc ggacctggca
     3061 tagtaggagg ccgagcacga acaggaaaat tgatgtcgag tttctcatcg gatctccgtt
     3121 attcccaagt ggatagcccc cagtaggata ttgttgggac ctcttcgatt caaggaataa
     3181 cgatcggagg gggggtgggg aaccccgtgc gggttcgagt ccccccttcg gcaccatcga
     3241 atcactcaat gggaatccgg aatgccatca agttaccttt attcttaaaa cgagccgagt
     3301 ggctgatttg tcgctccgag caactcccgg tcccagaaag attactgcta caccgcgcgg
     3361 aggaggaaaa gcaacgggac gggcgtaatc gccagacaga ccaggagcac gtatttcgag
     3421 aacgggaaca gccggctcat cccggcgatg agcgcgatac cgcctccgtc gccgatgatg
     3481 acgttgcccg gtaaattgaa cagaacggcg agaacgagat accggtgatg gagaaggaag
     3541 gggacgatcc tcgacggcgt gctccgtgcg aggaaccgga aacgctcctc cgcgcttagc
     3601 ggctcgagtt ctgtcacgag cttccgggcc cgctcgagcc tcatccatcc cagggcgcgg
     3661 gcaatgtaac ggggcggaag gagccggccg gccaggaagc ttagcgaaag cgccagcacg
     3721 gtgcaaaggt agacgagaac aatacccccg acaccgagca tcgccatgag cataaggccg
     3781 atttcgatgc cgggcatgaa cgggagcgcc atgaggaaga cgtacaggac ggctgagccg
     3841 aatacgatga agacggccgt atcgacatca ctgggccaca tctggaaggc gaggaggtcc
     3901 gagagcctcg ccacaccgta gttcaggaac aagaggatgc caaggaagag gagaaccccg
     3961 ctcgcgagct tggtgtatcc ccgagaaacc tcgatgtccg cgatctgtgt catggcggtc
     4021 gtgacaagga aaaaaggtga caggcatggc cggcccacgg cgaccgccca ttcctgcttt
     4081 cacgaaccgg gattcctgtt gtcattctcg cattccttat tgctgtatcc ggaccggaag
     4141 ggtgcgacct cgcccgtttc cggctcgaag cggtgacgcc agacgcaacc cacatcattg
     4201 cccagcaggt gaagggagat agatggttcg tcgctggtgg tgacgacccg gtggatgtcg
     4261 cctttcggag ggattaaatc gtaaaatgat ccaacgctta ggtgtttttt ggcgaccagt
     4321 gtcagatcgg tatgacggtc atcaggaccg accggcgtgg ctgcttcata gatctcctca
     4381 tcctgctcgc ccacatataa ccccaccaaa ccccacgcaa ggtggtcgtg gacaggtgtg
     4441 gctgcaccat ggggcagcac cagggcgcaa agggaaagag tcccttccgt atcgcggtac
     4501 agaagccagt ttgcgatccc tttgcccata ccccctgctt ctggagtacg gcggaactcc
     4561 gccggcagcc agtcgggatc ggccatcatc tggctgaaca gggggcggat ggccgccaat
     4621 cgtgcctcga ccgaagcggg gccggaaacg atttccttga ccgctcctat gaaacgtcgt
     4681 acctcgctgg tgtcacagat aaactcgtca gccatgtaag ttcttattcc ctccgattct
     4741 catttttagg ttgccccctg taccaatatt cctttttgat tttccagtac cagcgctcgc
     4801 gggtccaaac gctcgtcgct tcgatggcgt tgtccttgac ggttccgacc cagaccatcg
     4861 tgccgtgtgt cgggctgtgt gtctctgtgt ggaagcggat cgcggcgccg tcaacggtgg
     4921 cctgatacga agcgtccttg aaaccgaacg ggctgcaccc ctccgagagg aatcttccgt
     4981 cgcgaaaaat aatggtgtcc ttgttgctcg cctgtttccc tttttctccc gattgggcga
     5041 cgaacgtctt tccgtcgagg aggcttacgg ttggaacggc acttggcgat gccgtggcgc
     5101 ctatcgcgcc cggaatcgca tccgcccccg tggcggtcac ggaaatcagg gcgatcgcga
     5161 tggctgccaa cgaggtagta gaaaggggag cgatcatctc cggtttctcc gttagaaaaa
     5221 atcatccatg ttattaacaa gattcggctt ggagcagcga ggtttcgtgt ttcggtctga
     5281 acgaggaagt gttcggccag accgacatgc ccctttttga tttcttctac acaacaaggg
     5341 gaatatctcc ggaagccgca cggactgcaa accccgcgca aacaactggc ttattggggc
     5401 aggaaaagac atattgtgga gagggtgctg atggaccaaa tttcttgggc gtttcgatag
     5461 gtttcgggga aacaatattg atgtgttgcg caatcccggc gaggaatgga atcggggtga
     5521 gcaaatgaag gttttgcgaa tccgttggca acgtctcgtg gatgaaaagt aacctatcac
     5581 aggagggcgc taaggagggc ggcgggagtg ccggaccgga tcggtttgga gggggaagga
     5641 ggtacgggcg ccactcgccg tcagaccacg gcatttgatg agatggtgat gcgaagtaat
     5701 tgtgaagttc gtgggcgtga tgatctctga catttggtac gacgatatcg acctcctcac
     5761 ggcaccctgt ggatcgaaga tcttccagaa ttccctcgca atttttcgaa ttcccgacct
     5821 tcccggcggc aaaccagttg ctggatatcg tttatcaccc cgataacata tagtttgtca
     5881 tacagcatca tctggcatga cctgtgcagt ttgtatactg tatacttatg taatagaaaa
     5941 attggccgtt atgaatttta gagtggcagc catgacgtgt cgtctggaag caggaggaag
     6001 gaggtggctc ggttcccctg aaatcgcggc aaacgcgagg ggagtgaatc gacctgaccc
     6061 catatctgga agtctctggt ccctgtttgc tcagcgtttc cagccgagga aagggcgaga
     6121 aagaaggagg gacagtccaa tgagtttccg gaggaggatc accagaaggc agttcatcaa
     6181 gggggcggcg atcgcgggtg gtgtgatgat gctgcctata aagtacatcg gcagcggcac
     6241 cgcgtacgcg ttcgctcaga gcccaggcct caaattatgg aaaacgaccc tccgcggtgt
     6301 aggccccgga ggaatcccgg tggcggacaa agatggattt accgcgcccg ttacgggagt
     6361 tacccatta
//
LOCUS       DASSLD010000464         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_2946,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000464 DASSLD010000000
VERSION     DASSLD010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2946"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="VFK23_08385"
     CDS             complement(<1..1159)
                     /locus_tag="VFK23_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET7319137.1"
                     /translation="MELTFNRLNSLSELDALRTRLKQEHAAKDVHRVRVCCGTACQAS
                     GSNKLIKKFEEEAATKSVDLEIVKTGCQGFCQRGPIVIQEPDDTFYQKVKQTDIPRLF
                     SDSVLNGRPYRKQLYRELPLSDPNTTMQDIPFYKKQKRVALYRNGIVDPCNIYDAIRE
                     EGYAALAKALSEMKPDEVIDIVKKSGLRGRGGAGFPAGLKWEHTKKSGAKVKAVVCNG
                     DEGDPGAFMDRSLMEGDPHSVIEGMVINAYAIGAQHGYIYVRHEYPLAVKNLGIAITQ
                     AEAMGLLGKNILGSGLDFTMEIKEGAGAFVCGESTALVASIEGERGMPRPRPPRLSEA
                     AGGLWGMPTNLNNVETFANVPTIITKGLDYYTSIGTEKSKGTKVFALTGKIK"
     gene            complement(1143..1631)
                     /gene="nuoE"
                     /locus_tag="VFK23_08390"
     CDS             complement(1143..1631)
                     /gene="nuoE"
                     /locus_tag="VFK23_08390"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HET7319138.1"
                     /translation="MLQQDEINKIDAILDDTSGQRGVLIAVLQRVQEKIGYLPEEAMK
                     MISERLNLSLTNIYGVASFYKHFHFKPRGKNVVKVCMGTACHVRGGKAVLAEMESKLG
                     IKEGETTNDLSVTLETVGCVGCCALAPVATVNDQDLYGELTPRMVEDIILAVRSEHGT
                     HV"
     gene            complement(1899..>2200)
                     /locus_tag="VFK23_08395"
     CDS             complement(1899..>2200)
                     /locus_tag="VFK23_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HET7319139.1"
                     /translation="FFAGTHILPLDRVQERYGRDKLGFLERGMKLGAEIAGFGDAGIR
                     LYPLPRVPVTMILWLEDDEFPSRATLFFDSTVDFQISLSDIVWSIAMMTTLAMFE"
BASE COUNT          473 a          625 c          584 g          518 t
ORIGIN      
        1 tcttgatctt tcccgtgagt gcaaagacct tcgtgccctt cgatttttcg gtgccgatcg
       61 acgtgtaata gtccagcccc ttggtaatga tcgtcggcac gttcgcgaag gtctcgacgt
      121 tgttcaggtt cgttggcatg ccccacaggc cgccggcagc ctcggaaagc ctcgggggcc
      181 ggggccgcgg cattccccgt tcaccctcga tggacgcaac cagggccgtg gactcgccgc
      241 atacgaaggc tccggcgcct tctttgatct ccatcgtgaa gtcgaggccg cttcccagga
      301 tgtttttgcc caggagtccc attgcctcgg cttgcgtgat ggcgatgccg aggtttttca
      361 ccgcgagcgg atactcgtgc cggacataga tatagccgtg ctgcgccccg atggcatagg
      421 cgttgatgac catcccttcg atcacggaat ggggatcgcc ctccatgagc gaccggtcca
      481 tgaacgcgcc cgggtctccc tcatcgccgt tacataccac ggccttaacc ttggcgccgg
      541 attttttcgt atgctcccat ttaagtcctg cggggaaccc cgcaccgccg cgcccgcgaa
      601 gacccgactt cttgacgatg tcgatcacct cgtccggctt catctccgag agggctttcg
      661 cgagggcggc atatccttcc tcgcgaatag cgtcatagat gttgcatgga tcaacgatgc
      721 cattgcggta cagcgccacc cgcttctgtt ttttgtagaa cgggatgtcc tgcatcgtgg
      781 tgttcgggtc tgacaggggc aactcccggt aaagctgttt ccggtagggc ctgccgttga
      841 gcacggaatc ggagaacagg cggggaatat ccgtttgctt caccttctgg tagaaggtgt
      901 cgtctggctc ctggatcacg atcgggcccc gctggcagaa cccctggcac ccggtcttga
      961 cgatctcaag gtcaaccgat ttagtcgcgg cctcttcctc gaacttcttg atcagcttgt
     1021 tcgaacccga ggcctggcag gccgtgccgc agcagactcg gacccgatgc acgtctttgg
     1081 ccgcatgctc ctgcttgagt ctggtgcgca gcgcgtcgag ctcggaaaga ctgttcagcc
     1141 gattaaacgt gagttccatg ttcgctcctc accgcaagaa tgatgtcctc gaccatccgg
     1201 ggggtcaatt ctccgtacag atcctgatcg ttgacggtag cgaccggggc cagtgcgcaa
     1261 cacccgacgc atcccacggt ttcgagcgtt accgaaaggt cgttcgtggt ttcgccctcc
     1321 ttgatcccga gcttgctctc catttcggca agcacagcct ttcctccccg gacatggcat
     1381 gccgtgccca tgcagacttt taccacgttc ttgccccggg gcttgaaatg gaagtgttta
     1441 tagaaggaag ccacgccgta gatgttcgtc agggacaggt tcagccgctc ggaaatcatc
     1501 ttcattgcct cttccggcag gtaaccgatt ttctcctgga ccctttgcag gacggcgatg
     1561 aggacccccc gctgtcccga ggtgtcatcg agaatcgcat caatcttgtt tatctcatct
     1621 tgctggagca cagtgctaac ctccctggta acatacgtta aataaataat ttcaataggt
     1681 tcttgaaagc attgagtgat gctgtaacca aattgcaatt cctgatacca tttgtaacaa
     1741 tagtgaaaaa acgcgaagtt gttcatccaa ggggagggta tattaacaac cgaaatgtaa
     1801 aaaatcaaga aaaaacagta ttaaaaaatc tcatgcaaaa attataaata cctattattt
     1861 taaaagttga tgggggtgtc cgggcagaag gcagcaagtt attcaaacat ggcgagggtc
     1921 gtcatcattg caatggacca gacgatgtcc gatagggaaa tctgaaagtc caccgtggaa
     1981 tcaaagaaga gggtggctcg ggaagggaat tcatcgtcct ccagccagag gatcattgtt
     2041 accggtacgc gcggcaaagg atagagcctg atcccagcat ctccgaagcc ggcaatttcc
     2101 gcccccaatt tcattccccg ctctaggaaa cccaatttat ccctgccgta cctttcctga
     2161 acgcggtcaa gggggaggat atgagtcccg gcaaaaaaac
//
LOCUS       DASSLD010000465         3706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1274931, whole genome shotgun sequence.
ACCESSION   DASSLD010000465 DASSLD010000000
VERSION     DASSLD010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274931"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..1966
                     /locus_tag="VFK23_08400"
     CDS             323..1966
                     /locus_tag="VFK23_08400"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HET7319140.1"
                     /translation="MIRNKFNRLLRLGFLTERELDSVERESKATGTAIEELMIRQGIP
                     KHEVLFCLSEYYGYPFVEFEESVAASHFLVLRLDLEHQKKARWFPLSVRDKKAEVVTC
                     CPDDKRVIEDIKKTLGVEEIDFRVALFQDVIRIIEHNFDVNRNFSISGGRTPLARVRT
                     FLAYRRSTYAHYRTLFAKGRTGLAFIRTGVSFIVIALLFLRILGAGLYLVLEAPLLVA
                     GVIMVYDGIKWYLPVRRIAGKPVDSTSTEPTWGTRVLNADLKEGQPVITRTGEIEGAA
                     ELRADWENLSPVMRRRFLASDRTDMAEERTSLAGHRTKLARARTGLAFARTGIAFTGL
                     GIGLMRQFQTGRWTILDVSLIVVGVLMSLEGFHWYFGGRRSGIEGFRSVLAVLKKETI
                     WDLMFPLGHKRFGAGKDRPAPPVTADQLPGIWATTGVALERTVLADRRGVMARLRTVM
                     AVSRTGMAYVRTGMSITSVGAGLLVYFGTASVGWTIFNAVLVIGGLALIADGFRWIIP
                     AERMRKEYPYCYGDMEITIPDYGKPAREWGTVVFNHDGE"
     gene            1971..3197
                     /locus_tag="VFK23_08405"
     CDS             1971..3197
                     /locus_tag="VFK23_08405"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion protein"
                     /protein_id="HET7319141.1"
                     /translation="MNSDRADTAVLPGSEFSLLVERGILSQAQLDKAAAAAQARGIAL
                     EKVLLSEYRVAKRNLLEALSAYYKCAFIEYDERLPIPPELLTGLSPDSPSTYQWMPVM
                     KEKTGKVVIAARYPESAAMRKDVQKHVRTDQYEFRVSLDMDIQWYIQDFLHAKVGLLI
                     GTERTGLAFWRNTMAHWRTRMACYRTDLAKARTGLAFMRWGLGTIALANALLQSKNAS
                     AHLFLYFLMLATGIAIALFGLPPYLKVRKSRMTPPGHQTLVEVTAATIQFLENYHFID
                     NSGVRRETQGTMLARMGDYLADHSTYLNPTPSSKTRTMFARERNVLAAQRTIAACYRT
                     IYARARTGLAFIRTGISFLGIGIGILSYFGFGLLTSFNIGLIAAGLLMLVDGLLWYWP
                     VRKEQAEVPRGIAYPE"
     gene            3306..>3706
                     /locus_tag="VFK23_08410"
     CDS             3306..>3706
                     /locus_tag="VFK23_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HET7319142.1"
                     /translation="MKIENKLILSNVINGAFIILIGFFAFQNMNLVLTKLRFVEIADD
                     LNASFLEMRLAEKNYFLYHDRQALAEVREKISYTMNTIEAEKKNIIPAIGEEDFQKLE
                     NYLNAYAGVVTRARGAIPEDREIELMIRGYG"
BASE COUNT          795 a         1030 c         1130 g          751 t
ORIGIN      
        1 ggacgcgatt aaaaggcgta ctaaagttaa agtgaaggat tcttggtatg cccgctgata
       61 gaagaaagaa atgaaggcat agaactcacg gacgaaaggt ccagagaaac aggggataca
      121 aaagagcaac gtcctcacgg gctgtcgaat gaaaccgaga gccgcaccgt ttgctctcga
      181 cgccttatgg attcgaacag aaaagccgct acacgccgaa aatagacttt gcgaacgtca
      241 cccaccgatt tctcttgtcc cgtcggatag aattctttac aataacgcct tgatcccgta
      301 tgataaatca atgggatatt ctatgatcag gaataaattc aacagattgc tccggctggg
      361 gttccttaca gaacgcgagc tggactccgt cgagcgggaa tcgaaggcca cggggacggc
      421 gatagaggaa ctgatgatcc ggcaagggat cccgaagcac gaggtcctct tctgcctgtc
      481 cgaatattac ggctatccgt tcgttgaatt cgaagaaagc gtcgccgcgt cccactttct
      541 ggttctccgg ctcgacctcg agcaccagaa gaaggcccgg tggtttccgc tctccgtgcg
      601 tgacaagaag gccgaggtag ttacctgctg tccggacgat aaacgggtga tcgaagacat
      661 taagaagaca ctgggagtgg aggagatcga ttttcgtgtc gcgcttttcc aggatgtcat
      721 ccgcatcatc gaacacaact tcgacgtgaa ccgcaatttc agcatctccg gcggccggac
      781 gcccctggcg cgcgtgagga cgttccttgc ctaccgcaga tcgacctacg ctcattaccg
      841 tactctcttc gccaagggga ggacgggtct cgcgtttatc cggaccggcg tctccttcat
      901 cgtcattgcc ttgctttttc tgcgcattct gggcgccggt ttgtacctgg tcctcgaagc
      961 gccactcctc gtggctggcg tcatcatggt ttatgacggc atcaaatggt atctaccggt
     1021 tcgcagaatt gccggcaaac ccgtcgattc gacgtcgacg gagccgacgt ggggcacgcg
     1081 cgtgctcaat gccgatttga aggaaggcca gcccgttatt acccggaccg gagagataga
     1141 aggagcggcg gagcttcggg cggactggga gaacctgtcg ccggtgatga ggcggcggtt
     1201 cctcgcgagc gaccggaccg acatggcgga ggaacggacc tcactggccg gccacaggac
     1261 gaagctcgcc cgggcgcgga cagggctcgc tttcgcacgc accggcatcg cctttaccgg
     1321 gctcggcatc gggttgatga ggcagtttca aacgggcagg tggaccatcc ttgacgtgtc
     1381 gcttatcgtt gtcggcgtcc tgatgtccct ggagggattc cactggtatt tcggcgggcg
     1441 gaggtccggc atcgagggct tccggtccgt gctcgcggtc ctgaagaagg agaccatctg
     1501 ggatctaatg ttcccgcttg gacacaaacg attcggggca gggaaggaca ggcccgctcc
     1561 cccggtcacc gcagaccagc tgccgggcat ctgggcaaca accggggtcg ctctcgagcg
     1621 gaccgtactt gccgaccgca ggggcgttat ggcaaggctc cggacggtca tggcggtttc
     1681 ccgcacggga atggcgtacg tcaggacagg aatgagcatt acctcggtgg gggccggcct
     1741 cctggtctat ttcggaacgg cctcggtcgg gtggacgatt ttcaacgcgg tgctcgttat
     1801 cggcggtctt gccctgatcg ccgacggttt ccgctggatc attccggctg agcgcatgcg
     1861 gaaggaatat ccgtactgtt acggagacat ggagataacc attcccgact acgggaaacc
     1921 ggcccgcgag tgggggacgg tcgtttttaa tcacgatggc gaatgatctg atgaatagtg
     1981 atcgcgcgga caccgcagtt ctgccgggat cggaattctc cctcctggtt gagcggggca
     2041 tcctgagtca ggcacaactg gataaggcgg cagccgctgc gcaggcgcgg ggaatcgctc
     2101 tcgaaaaggt tctgctcagc gagtaccggg tggccaagcg gaacctgctc gaagcgctgt
     2161 ccgcatacta caaatgcgcc ttcatcgagt acgacgagcg gctgccgatt ccgcccgagc
     2221 ttctgaccgg cctcagcccc gactcgccct cgacgtacca atggatgccc gtgatgaagg
     2281 aaaagacagg caaggtggtc atcgcggcga gatatcccga gagcgcggcg atgcggaagg
     2341 acgtgcaaaa gcacgtgagg accgatcaat acgaattccg cgtttccctc gacatggaca
     2401 tccaatggta catccaggat tttctccatg cgaaggtcgg gcttttgatc ggcactgagc
     2461 gtaccgggct tgcgttctgg cggaacacca tggcgcactg gcgcacgaga atggcctgct
     2521 accggaccga tctcgcgaag gcacggaccg gccttgcctt catgcgatgg gggctgggca
     2581 ccattgcgct cgcgaatgca ttgctgcaat cgaaaaacgc gagcgctcat ctcttcctgt
     2641 attttctcat gctcgcaacc ggcatcgcga tcgcgctgtt cggccttccc ccatacctga
     2701 aggtccggaa atcgcggatg accccccctg ggcaccagac gctggtagag gtaacggctg
     2761 cgacgatcca gttcctcgag aactaccatt ttatcgacaa ttcgggtgtc aggcgcgaga
     2821 cgcaggggac gatgctcgcg cgcatgggcg actatcttgc cgatcacagc acctatctca
     2881 atccgacgcc gtcgagcaag acacgcacca tgttcgcgcg agagcggaat gtgttggcgg
     2941 cccagcggac gatcgcggcg tgctatcgca ccatctatgc gcgtgcgagg accgggcttg
     3001 cgttcatacg gacgggcatt tccttcctcg gcatcggcat cggtattctg agctatttcg
     3061 gattcggcct gctcacctcc ttcaacatcg ggctcatcgc agcggggctt ttgatgctcg
     3121 tggacgggct gctctggtac tggcctgtcc ggaaggaaca ggcggaagtg ccgaggggca
     3181 tcgcgtaccc ggagtgaggg acgcataggg catggcgcat ggcgtaatga aaaagcgctg
     3241 agcgcaaagc gtggagcgaa cagagtcgaa cgctatgcgc tttgcgccac ggctgtgcgg
     3301 ttcttatgaa aatagagaac aaactcatac tctcgaatgt catcaacggc gccttcatca
     3361 tcctgatcgg ttttttcgct tttcagaaca tgaacctggt actgacgaag ctcaggttcg
     3421 tggagatcgc ggatgacctg aacgcttcct ttctcgagat gaggctggcg gagaaaaact
     3481 attttctcta ccacgaccgg caggccctcg cggaggtgcg cgaaaagatc tcgtacacga
     3541 tgaataccat cgaggcggaa aagaaaaata tcataccggc catcggggag gaggattttc
     3601 agaagctcga aaactatctg aacgcttacg cgggcgtcgt tacgagggcc cgtggcgcta
     3661 tcccggagga ccgggagatt gaattgatga tcaggggcta cggcaa
//
LOCUS       DASSLD010000466         3556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_452478,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000466 DASSLD010000000
VERSION     DASSLD010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452478"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VFK23_08415"
     CDS             complement(<1..355)
                     /locus_tag="VFK23_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7319143.1"
                     /translation="MVDPKDISRISLFEGLNDAERISIANVMTGDTFPEGHVVYRENE
                     VGCSCVYIIKRGKVDVLKMSTDGDPLTLAVLKEGNFFGEFSFFDHKPHSASTVVSAPD
                     TVVLSLQRPDFDQVAE"
     gene            614..1267
                     /locus_tag="VFK23_08420"
     CDS             614..1267
                     /locus_tag="VFK23_08420"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET7319144.1"
                     /translation="MRIILLGAPGAGKGTVAKLLTQLDGSVQISTGDILRGAVKAGTP
                     LGKEAQGYMDRGDLVPDSLIMGIMEQRLQEKDCQKGFLLDGFPRTIPQADALGALLTK
                     LNIKLDLVANIEVPRDVILDRLTTRRTCSNPSCQEIYNVKSNPPAPGDKCKKCGSPVV
                     QRADETVEAISKRLDVYNDKTAPLIGYYEKKGMLLTIRETSSDGVVNAIKKGIAAKK"
     gene            complement(1554..2372)
                     /locus_tag="VFK23_08425"
     CDS             complement(1554..2372)
                     /locus_tag="VFK23_08425"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HET7319145.1"
                     /translation="MSKKTIYIISDATGQSALHILRAALVQFDHARAKIMVFHDIDSK
                     ASLTRILEQALQARALIAFTFVKKGMRDLASAFCRKHDILHLDILGPLINNLSSYLRL
                     EPLETPSLLRKVDERYFQRIEAIEYTVDHDDGRDTARLKDADIVILGLSRTSKTPTSF
                     FLAQEGYKVANVPIVPGIDLPEELDEIDQNKIVCLNIDAAVLQKVRKVRQKQFGLKPS
                     YSEFKTVFGEVEYVLDLVRKHRAWKIVDTTNKSIEETAWEIIHAVYGDEHDEYT"
     gene            2658..>3556
                     /locus_tag="VFK23_08430"
     CDS             2658..>3556
                     /locus_tag="VFK23_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HET7319146.1"
                     /translation="MDLSPKTKVNDVISRYPFLKDFLVSVNPEFKMLDNPFMRKTVGR
                     IASLGKAAMIGGMDVKKLIDDIAGEIRKKTGEAVAVSYGDGGPDEQDLKIDQMKEIIK
                     ELHAGKDAESQKQKFHDLIKDVAPWEIAQMEQKLIAEGMPETEIKSLCEVHVSVFKEA
                     LEHKTVPGLPAGHPVHTFMLENRACEGILKEAEAITDYTKEKDKLLAIIDRLADIDKH
                     FLRKENQLFPIIETKGITGPSKVMWALHDDIRNFIKDVRKRVKDGKMEKVAVDAMVKM
                     VNDMIYKEEHILFPMTLETLSEE"
BASE COUNT          849 a          961 c         1010 g          736 t
ORIGIN      
        1 tctccgccac ctggtcgaag tccggccgct ggagcgacag cacaacggta tcgggggccg
       61 aaacaacggt gctggccgaa tggggcttgt ggtcgaagaa ggaaaattct ccgaagaaat
      121 tgccttcctt tagcaccgca agggtgagag ggtcgccatc ggtgctcatc ttcaacacgt
      181 ccaccttgcc gcgcttgatg atgtagacgc acgagcatcc gacctcgttt tcgcggtaga
      241 ccacgtggcc ttcgggaaaa gtatcgccgg tcatgacgtt tgcgatgctt atgcgttcgg
      301 catcattcag cccttcaaac agggatatcc tcgatatgtc ttttggatcg accatggaaa
      361 cctccgcgag gttaaaaagc ggcgggaagt gcagagtctc ttctcgcgtc cgtcaacgag
      421 atatatccgg tactgataat gcaaacctga aaatttattc agagaaaaaa gatgcaatat
      481 tttatgcgtg tgcctgacag gcacccttgt ctcaagtata tcactacatt tcgcttgatt
      541 ttccccctca atcgtacgat aatggccgcc ttgaaacgcg atattactat cctatgctag
      601 gaggttcggt acaatgagga tcattctttt aggcgcgccg ggcgcgggaa agggcacggt
      661 tgcgaagctt ttgactcagc tcgacggctc ggtgcagata tcgaccggag acatccttcg
      721 cggcgccgtg aaggcgggca cgccgctcgg caaagaagcg cagggctaca tggaccgggg
      781 cgaccttgtc cccgattcgc ttatcatggg gatcatggag cagcggctgc aggagaagga
      841 ctgccagaag ggctttctcc tggacggctt tccccggacc ataccgcagg ccgacgcgct
      901 gggggcgctc ctcacgaaac tcaacatcaa gcttgacctc gtggcgaaca tcgaggtgcc
      961 ccgcgacgtg atcctggacc gcctgacgac ccgccgcacc tgctccaacc cgtcctgcca
     1021 ggagatctac aacgtgaaga gcaacccgcc tgcgccagga gacaagtgca agaagtgcgg
     1081 aagcccggtg gtgcagcgcg cggacgagac cgtggaggcc atttccaaga ggctcgatgt
     1141 ttacaacgat aagaccgcgc ccctgatcgg ctactacgag aaaaagggaa tgctgctcac
     1201 gatccgcgag acatcgagcg acggagtcgt gaacgcgatc aagaagggaa tcgccgcgaa
     1261 aaagtgacgg cgaagccgtc atcctgagcg tagcgaagga tctcgctcgt caccccttcg
     1321 acaggctcag tgcaggctcc gagcgaggcg aggggccgtc atcctgaata tcacgaaagg
     1381 tattagcggt ctcttcgata aactcggggc gaattccatg aaggaactgg ctgaggaaag
     1441 cagttcataa attcataagc agccaaacac gaaggggtta cggaattgcc gtaacccctt
     1501 ttgtttggcg atttcatttc tttacccata cgcctgatga gtcctagccg gagctaagta
     1561 tattcatcgt gttcatcgcc gtaaacggcg tggatgatct cccacgcggt ctcctcgatg
     1621 gacttgttcg tcgtatcgac gatcttccag gcccggtgct tcctgacaag gtccaagaca
     1681 tactcaacct cgccaaagac cgttttgaac tcgctgtagc tgggcttgag gccgaactgc
     1741 ttttggcgga ccttccgcac cttctggagg accgcggcgt cgatattgag acagacgatc
     1801 ttgttctggt cgatttcatc gagttcttcc ggcaaatcaa tgcccggaac gatcgggaca
     1861 ttcgcgacct tgtagccctc ctgggcgagg aagaacgacg tcggcgtctt ggacgtccgc
     1921 gaaaggccga ggatgacgat gtcggcgtcc ttcaggcgcg cagtgtcccg gccgtcgtcg
     1981 tggtcaacgg tatactcgat cgcctcgatg cgctggaaat accgttcgtc gacctttcgg
     2041 aggaggctcg gggtctcgag cggctccagc cgcagatagg aggagagatt gttgatgagc
     2101 gggccgagga tgtcgagatg gaggatgtca tgcttccggc agaatgcgct ggcaaggtcc
     2161 cgcatcccct ttttgacgaa cgtaaaggct atgagtgccc ttgcctgtag ggcctgctcg
     2221 aggatcctgg taaggctggc cttggaatcg atatcgtgga agaccatgat tttggctcgg
     2281 gcatgatcga actgcaccag ggcagccctc aggatatgca gggcgctctg gcccgtggcg
     2341 tcggatatga tgtagatcgt tttcttcgac atttttttct ccctcagaac tatatatacc
     2401 acaacggcgc gaagacaaaa agagaatcct ggaaaaggca gtcgaccgcg aacgaactca
     2461 aacaatcgcg aattgcgtca actatgtcta aaagggattt acccgcggta agatattttc
     2521 tcttttcggc cttctgattc tattcgcgat aattcgggtc ttattcgcgg ttaatcgctt
     2581 ttttcaggat tttcatgacg aaaatcatac gcattcccat ccgcgcgtgc tatagttgat
     2641 aaaaaaagga ggccgccatg gacctgagcc cgaagacaaa agtcaacgac gtcatatccc
     2701 gctacccttt tctgaaggac tttctggtca gtgtaaatcc cgaattcaag atgctcgaca
     2761 atcccttcat gagaaagacc gtgggcagga tcgcctccct cgggaaggcc gccatgatcg
     2821 ggggcatgga cgtcaaaaag ttgatcgacg acatcgccgg ggagatcagg aagaagacgg
     2881 gcgaggcggt cgccgtgtcc tacggcgacg gcgggcctga cgagcaggat ctgaagatcg
     2941 accaaatgaa ggagatcatc aaggaactcc acgcaggcaa ggacgcggag tcgcagaaac
     3001 agaagttcca cgacctgatc aaggacgtgg cgccctggga gatcgcccag atggagcaga
     3061 agctcattgc cgaaggcatg cccgagaccg agatcaagag cctctgcgag gtgcacgttt
     3121 cggtcttcaa ggaggcgctg gagcacaaga ccgtgcccgg tcttcccgcc ggccatcccg
     3181 tccatacctt catgctcgaa aaccgcgcct gcgaaggcat cctcaaggaa gctgaggcga
     3241 tcaccgatta cacgaaggaa aaggacaagc ttctggccat catcgaccgt cttgcggaca
     3301 ttgacaaaca cttcctgcgc aaggagaacc agctcttccc catcattgag accaagggga
     3361 taacggggcc gtccaaggtc atgtgggcgc tccatgacga catccgcaat ttcatcaagg
     3421 acgtccgcaa gcgcgtcaag gacggtaaga tggagaaggt ggctgtcgat gcaatggtca
     3481 agatggtgaa cgacatgatt tacaaagaag aacatatcct cttccccatg accctcgaaa
     3541 ccctgtccga ggagga
//
LOCUS       DASSLD010000467         3627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_307016,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000467 DASSLD010000000
VERSION     DASSLD010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307016"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /gene="murF"
                     /locus_tag="VFK23_08435"
     CDS             complement(<1..1073)
                     /gene="murF"
                     /locus_tag="VFK23_08435"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET7319147.1"
                     /translation="MNAPDNKLIEEILGSVERAEITGVSIDSRTIKSGELFIALKGDR
                     FDGHDFVPDVVRRGAWGALVERAALESRFASFSGLKNILPVEDTLHALQEMSRRHRRK
                     FPIPVVGITGSNGKTTTKEMLAGILKQTGPVLKNEGNLNNHIGVPLTLMKLNKSHRAA
                     AVEMGMSAPGEIDLLSRLVHPTVGVITNIGPAHLEFFGTIEKVAEAKGELLGNLASDA
                     AAVLNADDPFLGAFRNKFSGRVLTFGIKSRADVSASKIGHGADHADFTLSAGGGSVVI
                     RLRAPGTHNIYNALAAAAAALAAGMPLDAVRSGLNDFAPVAMRSEIKEIRGRTVLADY
                     YNANPASMEAAISTLASLSAGRKS"
     gene            complement(1139..2686)
                     /locus_tag="VFK23_08440"
     CDS             complement(1139..2686)
                     /locus_tag="VFK23_08440"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET7319148.1"
                     /translation="MKLKDLLEVLERTPVLPGGLPDREVPAIAHDSRKVGPGSLFVAV
                     RGFHADGHRFIPQAVRQGAIAVVAEEGGDIGPSGGTPVIRVPDSRRALARLAANFYGY
                     PSRRIKVVGITGTKGKTTTSYLVKSVIEAAGHTAGLIGTIDYRIGGKVYPAPNTTPES
                     LDLQALLAEMAGLKTEYCVMEVSSHALALGRTEGCVFEAAAFTNLAHDHLDFHDTLDD
                     YFQAKLLLFTGLGPDMPAVVNIDDERSAEIVNHTKARVITFGLSGNADVHPKGSVSHG
                     ISGLSFTAGTPAGDIAIRSPLVGKHNTYNILTAVALGVGLGFSDGTISAGIGRMQSVP
                     GRMEKVDDGQPFGVLVDYAHTEGSLVSLLDAVRGIAAGRVITVFGCGGDRDRTKRPKM
                     GAAALNGSDVVIVTSDNPRTEDPLAIIREIEGGMSSAGARVSSLSGTKKSASGRTPYL
                     VIPDRRAAIAAAVSIAEAGDVVVLAGKGHEDYQIIGETKVHFDDREVAREEIRRRNAE
                     CGMRNAG"
     gene            complement(2683..>3627)
                     /locus_tag="VFK23_08445"
     CDS             complement(2683..>3627)
                     /locus_tag="VFK23_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET7319149.1"
                     /translation="RGRPAFNPNEPQRYRPEEWRNRAVTDAFEPGSIFKPVLAAAVLE
                     ERVVHPLEQFDCSGGRIQLADRVIKDAHEHGVLTFTDVIAQSSNVGTIKAALRLGKDR
                     FYKYITAFGFGRRTGVDLPGEIPGLLRESRQWSGVSLGEIAIGQEIGVTPIQMAAAYS
                     ALANGGTLMKPYIVAEIVDREGKGRKFAPQAVGRVVSAATCTQVNKMLRRVVEAGTGQ
                     KAHPSGYTAAGKTGTAQKIDRRTGRYSKKDYVSSFVGFVPAASPKFVIVVMIDSPEGD
                     VYGGSVAAPVFKAVAEQGLAYLQVPPDEEGGQMLLVNR"
BASE COUNT          609 a         1194 c         1111 g          713 t
ORIGIN      
        1 gatttcctgc ccgccgaaag agaggccagg gtcgatatcg ctgcctccat ggaggcggga
       61 ttggcgttat agtaatcggc aaggaccgtc ctgccccgga tctctttgat ctccgaccgc
      121 atggcgacgg gagcgaaatc attgagcccg gacctcacgg cgtcgagggg catgcccgcg
      181 gccagtgcgg ccgcagccgc ggcaagggcg ttgtagatat tgtgcgtccc cggggcgcgg
      241 agccttatga ccaccgatcc gccgcccgct gacagcgtga aatccgcgtg gtccgcaccg
      301 tgcccgatct tcgaggcaga cacgtccgct ctgcttttga ttccgaatgt gagaacgcgg
      361 ccgctgaatt tgttcctgaa cgcgcccagg aaaggatcgt cggcgttcaa gaccgcggcc
      421 gcgtcggaag caaggtttcc gagcagttcg ccctttgcct ccgcaacctt ttcaatagtg
      481 ccgaaaaact cgagatgcgc cggcccgata ttcgtgatca cgcccaccgt gggatggacc
      541 agccgggaga gcagatcgat ctctcccggc gcgctcatgc ccatctcaac agcggcggcg
      601 cgatggcttt tattgagctt catgagggtg agcggcacgc cgatgtggtt gttcaggttc
      661 ccctcgttct tgaggaccgg gccggtctgc ttcaggatgc cggcgagcat ttccttggtc
      721 gtggtcttgc cgttcgatcc cgtgataccc acgaccggga tcggaaattt tctgcggtgc
      781 cggcgcgaca tctcctggag ggcatgcagg gtgtcttcga cgggcaggat attcttcagg
      841 ccgctgaagc tcgcaaacct gctttcgagc gccgcgcgtt caaccagcgc cccccaagcc
      901 ccccgccgaa ccacgtcggg aacgaaatcg tggccgtcaa agcggtcccc cttgagggcg
      961 atgaagagct caccgctctt gatggtccgg gagtctatgg acaccccggt tatctcagcg
     1021 cgttccacgc ttccgagtat ttcttcaatc agcttgttgt caggcgcgtt catgattcga
     1081 atccggaatg cggaattcgg aatgcatagg agcggagaaa aaccacgatg ccttgatttt
     1141 atcccgcatt acgcattccg cattccgcat tgcgtctcct gatttcttcc cttgccactt
     1201 cccggtcgtc aaagtgcacc ttcgtctcgc cgatgatctg ataatcttcg tgccccttgc
     1261 cggcgagaac gacgacgtct cccgcctccg ctatgctgac cgcggcggcg atcgctgcgc
     1321 gccggtcggg aatgaccaga tagggcgtcc tgcccgatgc gctcttcttc gttccggaga
     1381 gggaggaaac cctggcgccc gctgatgaca tgccgccttc gatctccctg atgatggcga
     1441 gcgggtcctc ggtcctcgga ttgtccgagg tcacgatgac gacatcgctc ccgttgaggg
     1501 cggcggcgcc catcttcggc cgcttggtcc ggtcgcggtc cccgccgcag ccgaatacgg
     1561 tgatgacccg gccggcggca atcccccgga ctgcgtccag gagcgacacg agggacccct
     1621 cggtgtgggc ataatcgacc agcacgccga acggctgccc gtcgtccact ttttccattc
     1681 ttcccgggac tgactgcatc cgcccgattc ccgctgagat cgtcccgtct gaaaatccga
     1741 ggccgacgcc cagcgccacg gccgtcagga tgttataagt attgtgcttc cccaccagcg
     1801 gcgaccggat cgcgatgtct cccgcgggcg ttcccgcagt gaaggaaagt ccgcttatgc
     1861 cgtggctgac gctccccttt ggatggacat cggcgtttcc ggaaagcccg aaagtaatga
     1921 cccgcgcctt cgtatggttg acgatctcgg ccgaccgttc gtcgtcgatg ttcaccacgg
     1981 caggcatgtc cgggccgagc cccgtgaaaa ggaggagctt tgcctggaag taatcgtcga
     2041 gggtatcgtg aaaatccagg tgatcgtgag cgaggttcgt gaacgcagct gcctcgaaga
     2101 cgcacccttc agtccttccc agggcaaggg cgtgggagga cacctccatc acgcagtatt
     2161 cggtcttgag gccggccatc tcggcgagga gggcctgaag gtccagggac tcgggcgtgg
     2221 tgttcggcgc gggatagacc ttgccgccga tgcggtaatc gatggtcccg atgagcccgg
     2281 ccgtatgccc ggcggcctcg atgacggact ttaccaggta gctggtcgtc gtcttgcctt
     2341 tcgtccccgt gataccgacc accttgatcc tccgcgaggg gtatccgtag aagttggcgg
     2401 cgagccgggc cagggccctg cgggaatcgg ggacgcggat gaccggagtg cccccggacg
     2461 gcccgatatc gcccccttcc tccgccacca cggcgatcgc accctgccgg accgcctgcg
     2521 gtatgaaccg atggccgtcc gcgtgaaacc cccgcacggc cacgaacagg gagccgggac
     2581 ccactttccg ggaatcgtgc gcaatcgccg gcacttctct gtccggcaga ccgccgggca
     2641 ggaccggtgt cctctcaagg acctccagga ggtctttcag tttcatctgt tcaccaggag
     2701 catctggcct ccctcttcat cgggcggcac ctgcagatag gcgagtccct gctccgccac
     2761 ggccttgaac accggcgcgg cgacgctgcc tccatagacg tcaccttcag gcgaatcgat
     2821 catcaccacg attacgaact tcggcgaagc ggcaggaacg aaccccacga aggagctgac
     2881 gtagtctttc ttcgaatatc tgccggtccg tcggtcgatc ttctgcgccg tcccggtctt
     2941 acccgcagca gtataccccg atgggtgggc cttctggccc gtgcctgcct cgacgacccg
     3001 ccggagcatt ttgttcacct gggtgcaggt cgccgcgctc accacgcggc caacggcctg
     3061 gggtgcgaat ttccgtccct tgccctcccg gtcgacgatc tccgccacga tgtagggctt
     3121 catgagtgtt ccgccgttcg ccagcgcgct gtacgcagcc gccatctgga tcggtgtgac
     3181 gccgatttcc tgcccgatgg cgatctcgcc gagggacaca ccggaccact ggcggctttc
     3241 ccggagcagg cccgggatct cgcccggaag gtccacgccg gtcctgcgtc cgaaaccgaa
     3301 ggcggtgatg tacttgtaga accggtcctt gccgagccgg agcgccgcct tgatcgtgcc
     3361 cacgttgctc gactgggcaa tcacgtcggt gaaggtcagg acgccgtgct cgtgggcgtc
     3421 cttgatcacg cggtcggcga gctggatcct gccccccgaa cagtcgaact gctcgagagg
     3481 atggaccacc cgctcctcaa gcaccgccgc ggccaggacg ggcttgaaaa tcgagcccgg
     3541 ttcgaaggcg tcggtcacgg cccggttccg ccattcctcc ggccggtagc gctgcggttc
     3601 gttcggattg aacgccggcc ggcctcg
//
LOCUS       DASSLD010000468         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_984724,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000468 DASSLD010000000
VERSION     DASSLD010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984724"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..1021
                     /locus_tag="VFK23_08450"
     CDS             353..1021
                     /locus_tag="VFK23_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319150.1"
                     /translation="MRIIMMFAALLVLAAGNAQAESGLPFQDFSLGPHYQTIKSDFAD
                     ASSAGTMYGLDLAINGSYADTLLDGQHNRLRFGDYLGVHTTLSGANYKSSGSRYFAGL
                     GYALGAQAGLAVSDYADIGARYYVDFRDNKFGSLNNSQSLRLNTAQLMVRLAKIYIAA
                     GAGNGKKAQGDETFRSSTFHVRYLFADGGYAGVMMDSLKIKYELTGRNDTITNIVVQF
                     GIAH"
     gene            complement(1062..1928)
                     /locus_tag="VFK23_08455"
     CDS             complement(1062..1928)
                     /locus_tag="VFK23_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319151.1"
                     /translation="MKKNALHIIYHIAVVVLSAAIALSLSDIARFLTRKVLLYWSLIE
                     NQEVFLVSTEIATAILLIVMINAMVRSVKVRKYSRMAKTAGIAAVAPPATFLARREIK
                     KLKEEQGSGRSIMLISSTGFRTFVDSDGDLHGVLKNSREAKIMLLDPLSDGVTARARS
                     LAKPDITPEGFREQILRSIDFLKGLKASQKNIRLKLYPDVPLLKMAILGDYLCLQHYP
                     TGLHVRTMPEYVFKHDRNGSLFNLFYQYFVSRWFDASIPEYDLDTDELVYRNTCGNEV
                     KREKFKEVEMEL"
     gene            complement(1984..2337)
                     /locus_tag="VFK23_08460"
     CDS             complement(1984..2337)
                     /locus_tag="VFK23_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319152.1"
                     /translation="MARGISKMFLSILATVLLSTAVFEGVSAALPNAASHYPGQASPR
                     AGANERDVSQMIRILESRIGSHRMPAQTKEKLATMSEKEFRLVEALCYRIARSGDSPE
                     SDLALLLAAALVVLS"
BASE COUNT          495 a          674 c          678 g          553 t
ORIGIN      
        1 gatagtgcgg ccccaggctg aaatcctgga acggcaaagc ctacaccctc ttcgtcgggc
       61 ccgcgctcca ttccaaggtc ggcgtcctga ctttgacgac cagattactg ctggagaagc
      121 gtttgtcgcc cggccccggc aaagacggat atgaagcttc agtcggatta aggattccgt
      181 tcgacgcact gtgatcccgg gaaattcgcc ttccctgtgt cctgtcttct cccttcggga
      241 gaatcttaga gacaggcttg atttgccgac atgtggtttt ttcgtctgct cattttgccg
      301 ggatgacagg aaagtggacc cagcccctgc tggtccctaa aggagactat tggtgaggat
      361 aataatgatg ttcgcggcgc tgcttgtcct ggcagccggt aatgcgcaag cggaatcggg
      421 attgccgttc caggatttca gcctggggcc gcactatcag acaataaaga gcgattttgc
      481 ggatgcatcc tccgcgggga cgatgtacgg actggatctg gcgatcaacg gctcttacgc
      541 ggacacgctc ctggacggcc agcacaacag gcttcgcttc ggcgattatc tgggtgtgca
      601 tacgaccctt tcgggagcaa actataaatc gtccgggagc cggtattttg cgggtttggg
      661 gtatgccctc ggcgctcagg cggggcttgc cgtcagtgac tacgcggaca ttggcgcacg
      721 atattatgtc gatttcaggg acaacaaatt cggatccctg aacaactcgc agtcgcttcg
      781 tctgaatacc gcccagctca tggtgagact ggcgaagatc tatattgctg cgggtgccgg
      841 aaacggcaaa aaggcacagg gtgatgaaac cttccggtct tctacttttc acgtacgtta
      901 tctgtttgcc gacggcggct acgccggcgt gatgatggac agcctgaaga taaagtatga
      961 gctcacgggc aggaatgaca ctatcaccaa tatcgtcgtc caattcggca tcgcgcatta
     1021 gggggaaggg taagcggatt cctttttctt tgaaaatctc cctaaagctc catctccact
     1081 tccttgaact tctccctttt cacctcgttg ccgcacgtgt tgcggtagac cagctcgtcg
     1141 gtatccagat cgtattccgg gatggacgca tcgaaccacc ggctcacgaa atactggtaa
     1201 aaaagattga agagactgcc gttccggtcg tgtttgaaaa catactccgg catggtgcgc
     1261 acatgcaggc ccgtcggata atgctggaga cagaggtagt ctcccagaat agccatcttg
     1321 aggagcggca cgtccgggta gagcttcaac ctgatgttct tctgggaggc cttcagcccc
     1381 ttcagaaagt caatgctccg gaggatctgc tcccggaacc cttcgggggt gatgtccggt
     1441 tttgcaaggc tcctcgcccg cgcggtcacg ccgtcgctca gcggatcgag cagcatgatc
     1501 ttcgcctcgc ggctgttttt gagaaccccg tggaggtctc cgtcggaatc aacgaaggtc
     1561 cggaaacccg ttgagctgat cagcatgatg ctccggccgg acccttgttc ttccttcagc
     1621 ttctttattt ccctccgcgc aaggaaggtc gcaggcggcg ccacggcagc aattcccgct
     1681 gtcttcgcca tgcgggaata tttcctgacc ttcacgctcc gcaccatggc attgatcatg
     1741 acgatcagca ggatcgccgt cgcgatctcc gtcgagacga ggaacacctc ctggttttcg
     1801 atcagcgacc agtacaacag gaccttgcgg gtcagaaacc gggcgatgtc gctgagcgag
     1861 agcgcgatgg cggcgctcag cacgacgacg gctatgtggt atataatatg aagcgcgttt
     1921 ttcttcatgg cttccctgcc tcccggatga cttgtttcct cggctcacgg actctgctcc
     1981 tgatcaggac aaaacgacga gcgctgcagc gagcagcagc gcaaggtcgg attccggaga
     2041 atcgccggat cgggcaatcc ggtagcacag ggcttcgacc agccggaact ctttctcact
     2101 catggtcgca agcttctctt ttgtctgggc aggcatgcgg tggcttccga tcctgctttc
     2161 gaggattctg atcatctgcg aaacatcgcg ttcgttggcg cccgcgcgcg gcgaggcctg
     2221 tcccgggtaa tgggatgcgg cattgggcaa cgccgcagat accccctcaa acactgcagt
     2281 tgacagcagg actgttgcca ggatcgacag gaacatttta ctaatgcctc gtgccattga
     2341 cgtcctcctc ccgttgaatg atgtagacgc agaagttgca gaagctcaag agcagttgcc
//
LOCUS       DASSLD010000469         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1131568, whole genome shotgun sequence.
ACCESSION   DASSLD010000469 DASSLD010000000
VERSION     DASSLD010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131568"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2878
                     /locus_tag="VFK23_08465"
     CDS             <1..2878
                     /locus_tag="VFK23_08465"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET7319153.1"
                     /translation="LPQPLLVVFDEFNNITPTPDVRTLTKVFLESTPPQIRFMLVSRT
                     RPDIELARLRARQALGELSGNDLKFSDAETHELFNSVYGMPMPESETSAVNRLAEGWP
                     AGLVLMHEYLAGEPERGKKEALLGSGIEGFRTHVFDYLAQEVFSSLNRDLQRFLLRTS
                     ITDYLPLELMERLAGLPGPKKAVAASVRTVVGDLRARNLFVTALDDDASVIRYHALFR
                     EFLQRKLIAQTDPAVVRKLYSIAASYYEASGDSVRMVDLLISSGQFDQAVREIESRGL
                     ELIARGQIQTVLRWIQALPLDFGNRPWFLLYRATAFRFIDPGAALTFFDLALSRFRVA
                     KKARDRTLGRMLSLCGFIEACFYAGGNFKRMEKAAASAASLLKRRKGASPEARARLSL
                     AIGMAFLFIGQLRRAAEYLDQALEMFRASREYFYQVQSASYLAACCGYLGDFARCRAA
                     LGRGFEAIKSIPHEAGGDAGLHMAQAMAALFEGRFAEAQECIDHSFGLAHRHDLMVLT
                     FLSLDIGGWLKIAMGDYEAAEALLTKCKDLGEEHGNAFFTASSAHLLALSYLHQGKLD
                     LAATEAEFALSDRARSGSSLFRAASLAVCGAIDINRGRSARAERRLEGARRIFRRCEA
                     VHQEASVLLALAEASMRKKKGKKAMEFLEEGFRIGAERGFSYYFLYAPSELAALARTA
                     LRQGIAQDFCTTLLRRYDQPESAPRLTIRCLGGFSVFRDGEPIADREWKGKQARTLLK
                     RLVAERGRKCPRDVITDMLWPDASPDAQRTNLASLLYRMRKILDGPRIANESESCIIA
                     GPEHIDLNASFVQTDVDQFLDHLGKARRLKAAEPERSIEEYEKAFSLYRGDFLPDDLY
                     DEWAATARERMRARYLQALGDMAQTAESLGKIATAVEAYRRQFLADECNEDACRRLMT
                     HYLANGGRNEALRAYERCQLALMRSLDMEPEEQTKKLHRSIIGG"
BASE COUNT          591 a          905 c          878 g          557 t
ORIGIN      
        1 tcttccgcag ccgcttctcg tcgtcttcga cgaattcaat aacatcacac ctactccgga
       61 tgtccgcaca ctgacgaaag tattccttga atccacgccg ccgcaaatac ggttcatgct
      121 cgtgtcgcgc acccgaccgg acatcgaact tgcccggctc cgggcgaggc aggccctggg
      181 agaactttcc ggcaacgatc tgaagttcag cgacgccgag acccatgagc ttttcaactc
      241 cgtctacggc atgccgatgc ccgagagtga gacctccgcc gtcaaccggc tggccgaggg
      301 ctggcccgcg ggcctcgtcc tcatgcacga gtaccttgcc ggcgagcccg agcgcgggaa
      361 aaaagaggcg ctgctgggca gcggcatcga ggggtttcgc acccatgtct tcgactacct
      421 cgcccaggag gtcttctcaa gccttaaccg ggacctgcag cgctttctgc ttcgcacttc
      481 gatcacggac tacctgccgc tggaactcat ggagaggctg gcgggcctcc ccggtccgaa
      541 aaaggcggta gccgcctcgg tccgtactgt cgtcggagac ctccgcgcaa gaaatctctt
      601 cgtcaccgcc cttgacgatg atgcctcggt catacgctat cacgctctct tccgggaatt
      661 tctccagcgt aagctcattg cgcagactga cccggccgtg gtgagaaaac tctactccat
      721 tgccgcctcc tattacgagg caagcggcga ttccgtccgc atggtggacc ttctgatctc
      781 ctcgggccaa ttcgaccaag cggtccgcga gatagaatcg cgaggcctcg aattgatcgc
      841 ccggggacag atacagacgg tcttgcgctg gatacaggcg ctgcccctcg atttcggaaa
      901 ccggccgtgg tttctgctct atcgggcaac ggccttccgc ttcatcgacc ccggtgcggc
      961 cctgactttc ttcgatctcg ccttatcgcg gttccgagtc gcgaagaagg ctcgcgaccg
     1021 cacgctggga aggatgctct cgctctgcgg atttatcgaa gcatgctttt acgccggagg
     1081 gaacttcaag cgcatggaga aggctgcggc gtcggcagcg tcactcctca agcgcagaaa
     1141 gggcgcctca ccggaggcgc gggcgcggct ttcccttgcc ataggcatgg cgttcttatt
     1201 catcggacag ctccgccggg cggccgaata tctggatcag gccctcgaaa tgttccgtgc
     1261 aagccgggaa tatttttatc aggtccagag cgcaagctat ctcgccgcct gctgcggcta
     1321 tcttggcgat ttcgcgagat gccgggcggc gcttggacgg ggtttcgaag ccatcaaatc
     1381 aatcccccat gaggcgggcg gtgacgcggg cctgcacatg gcccaggcga tggcggctct
     1441 cttcgaaggc cggtttgccg aggcgcagga atgcatagat cacagttttg gtctcgctca
     1501 caggcacgac ctcatggttt tgactttcct gtcccttgac atcggcggct ggctcaagat
     1561 agccatgggc gattacgaag ccgccgaggc ccttctgact aaatgcaagg acctggggga
     1621 agagcacgga aacgcttttt ttacggcatc gtcggcgcac ctgctcgctc tcagttacct
     1681 gcaccaaggc aaactggacc tggcggcaac agaggcggaa ttcgcccttt ccgaccgcgc
     1741 ccgctccgga agcagtctct tccgcgcggc gtcgctcgcc gtctgcggcg ccatcgacat
     1801 taatcgcgga cgaagcgcgc gggctgaacg aaggctcgag ggagcgaggc gcatcttcag
     1861 gcggtgtgag gcggtccatc aggaggctag cgtcctgctg gccctcgcgg aagcgagcat
     1921 gcgaaaaaag aaggggaaaa aggcgatgga gttccttgag gaggggttca ggatcggcgc
     1981 agagaggggc ttcagctact atttcctgta tgctccgtcc gaactggcgg cgcttgcgcg
     2041 aacggccctt cgtcaaggca tagcgcaaga tttctgcacg acattattaa gacggtatga
     2101 tcagccggag tccgctcccc gcctcacgat acgctgcctc ggcgggttct ccgtctttcg
     2161 agacggcgag ccgatcgccg acagggagtg gaaagggaaa caggcccgga cgcttttaaa
     2221 acggctcgtg gcggaacgcg ggcggaaatg tccgcgggac gtcatcacgg acatgctctg
     2281 gccggatgcg tctcctgacg cgcagcggac caatcttgcg agcctgctct atcgaatgcg
     2341 caagatcctt gacggccccc gcatagcgaa tgaatcggaa tcctgcatca ttgccgggcc
     2401 cgagcacatc gacctcaatg cctctttcgt gcagactgat gtcgatcaat ttctcgatca
     2461 cctgggaaaa gccaggcgct taaaggctgc tgaaccggag cgcagcatcg aagagtacga
     2521 aaaggcgttc agtctttacc ggggagactt cctgcccgac gacctctatg acgaatgggc
     2581 tgccaccgca cgggagcgga tgcgagcgcg ctacctgcaa gcgctggggg acatggcgca
     2641 gacggcagaa tcgttgggaa aaatagcgac ggcggtagaa gcctatagaa gacagtttct
     2701 ggcggacgaa tgcaatgaag atgcatgccg ccggctgatg acccattacc tcgccaacgg
     2761 cggcaggaac gaagctctgc gcgcatacga acgctgccaa ctcgcgctca tgcgcagcct
     2821 ggacatggag ccagaggaac agacaaagaa actgcaccga agcattatcg gcggatgatc
     2881 ggcgatcatg cccgctcccg ccgtcccgac gcgcccctgc acacgctcca c
//
LOCUS       DASSLD010000470         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1551582, whole genome shotgun sequence.
ACCESSION   DASSLD010000470 DASSLD010000000
VERSION     DASSLD010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551582"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..839
                     /locus_tag="VFK23_08470"
     CDS             60..839
                     /locus_tag="VFK23_08470"
                     /inference="COORDINATES: protein motif:HMM:NF015566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET7319154.1"
                     /translation="MPLFDTHTVLLMFSVAVLGGIIGLDRTAAGQFMISQPIVAAPFT
                     GWVLGDLNAGLVIGAALELIWVLDMPVGTFVPADATVGAVSAAAIAALGSPGGAAPSF
                     IGFSILLTTAMVPVTMKAEGLVRARNSNLADRVLSASAEDRGRALMRAQFTGLAFFFL
                     KSFVLYVIFIPLGVAALGLFARLPAEVHTALSLYVKLLPVLGVALIVQKLSVKAFDQF
                     FFAGFVTAAVFGQAVHAPALVVLLLTAAAGWLGARYHERQP"
     gene            823..1602
                     /locus_tag="VFK23_08475"
     CDS             823..1602
                     /locus_tag="VFK23_08475"
                     /inference="COORDINATES: protein motif:HMM:NF015570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HET7319155.1"
                     /translation="MNDNRKVIGIFDLFAVFRRALLIQAAWSFDRMQTLGFAYAIEPA
                     LRKVYPDRAEYAERLKVHLEYFNTQPYLASFIMGAAVRLEEERATGRTEAPDVSRLKA
                     ALMAPLGALGDSLFWGALKPLAAAAAAAVLMTGSWWAPFLFLAIFNLWHLGLRMGMLF
                     WGYASGGDAVALMGKYHITKMAKRFKLMSLCILGGILGMMPFWRPEFNPSYHAPGVVV
                     SLSAMLLTLALVAVMRRGGSPVKFMLGLAALCVALAYAGAV"
     gene            1643..1912
                     /locus_tag="VFK23_08480"
     CDS             1643..1912
                     /locus_tag="VFK23_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET7319156.1"
                     /translation="MKEKELLIENKLGLHARAAAQIVKTASAYTSKILLAKDGLEVDG
                     KSIMGIMMLAAAQGSSVTVQAQGEDEAEAISGLEQLFKDKFGEKE"
     gene            1948..>3439
                     /gene="ptsP"
                     /locus_tag="VFK23_08485"
     CDS             1948..>3439
                     /gene="ptsP"
                     /locus_tag="VFK23_08485"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET7319157.1"
                     /translation="MHEKIYRGIVASPGIVIGRAYLLDRRKVVVAGRRIEDVSVKEEV
                     ARFKKAVELSKTQLEDVKKRISKGLGKSHLYILETHIMLLEDKMLVEGTIKRIRESLL
                     NAEGALKETIAAIGAKFDSIEDEYLRDRKHDVEQVGERVLRNLVGHKQESLADIKEEA
                     IIIAHDLAPSDTLVMRKDKILGFATDAGSRTSHTAILARSLGLPAAVGLESITASVKT
                     GDVVILDGIHGVVIVDPDEETFLDYLKKQRKYKYFEQELEKLKSLPAETLDGHVVYLQ
                     GNIELPEEATSTAEHGGTGIGLYRTEFLFMNRQTLPTEEEHYAAYRHVAERATPHEVV
                     IRTLDVGGDKIDMAGEFEKEANPALGLRAIRFCLKKRDIFRTQLRGILRASVLGNIKI
                     LVPMISGLTELYETKQMIEDVKAELRAEGRPFNEAVPVGAMIEIPSAAMIADLLARQV
                     DFFSVGTNDLIQYALAIDRINEQVAYLYEPLHPAVLRMLRTVVSAAH"
BASE COUNT          671 a          991 c         1108 g          669 t
ORIGIN      
        1 cgtttttttt gcattgcact ccgaagtccg aactttacac tccgaaccaa gggtctctta
       61 tgcccctttt cgacacccat accgtgcttc tgatgttcag cgtagccgta ctcggcggga
      121 tcatcggact cgacaggacc gctgcggggc agtttatgat ttcccagccg atcgttgcgg
      181 ccccttttac cgggtgggtc ctcggggatc tgaacgcggg gctggtcatc ggagcagcgc
      241 tcgagttgat ctgggtgctc gacatgcccg tgggaacgtt tgtacccgcg gacgccacgg
      301 tcggcgcggt gtcggcggcg gcgatcgcgg ccctcggaag tccgggtggg gcggcaccgt
      361 cgttcatcgg cttcagtatc ctcctgacga cggcgatggt cccggtcacc atgaaagccg
      421 aggggctggt gcgggcgagg aattccaatc tcgcggatcg cgttttgtcc gcatcggcgg
      481 aagaccgggg gcgcgcactg atgcgtgccc agtttaccgg cctggcgttt ttttttctga
      541 aatcattcgt gctgtatgtg atattcatcc cgctgggagt cgctgctctg gggctgttcg
      601 cccgtctccc cgccgaagtc catacggcgc tgtccctgta cgtcaagctc ctccccgttc
      661 tgggagtggc gctcatcgtg caaaagcttt ccgtgaaggc atttgatcag ttttttttcg
      721 ccggttttgt aacggccgct gtctttggac aggccgtcca cgcacctgcg ctcgtcgtgc
      781 tgctgctgac cgcggccgcg ggctggctgg gggcgagata ccatgaacga caaccgtaag
      841 gtcataggaa ttttcgatct gttcgccgta tttcggaggg cattgctgat ccaggcggcg
      901 tggagtttcg accggatgca gacgctgggg tttgcctacg ccatcgagcc ggcgctcaga
      961 aaagtctatc ccgaccgtgc cgaatacgcc gagcgcctca aggtgcacct tgaatatttc
     1021 aacacgcaac cgtaccttgc ctcgttcatc atgggcgccg cagtgcggct ggaagaggag
     1081 cgggcaacgg gaagaactga ggctccggac gtgtcccgcc ttaaggcggc gctcatggcg
     1141 ccgctcggcg ccctcggcga cagtctgttc tggggcgcgc tcaaaccgct ggcggcggcg
     1201 gcagcggcgg ctgtgctcat gaccggttcc tggtgggcgc cgttcctctt tctcgcgatc
     1261 ttcaacctgt ggcatctcgg tctcaggatg gggatgctgt tctggggcta cgcgagcggc
     1321 ggtgacgccg tggcgcttat ggggaagtac catattacga aaatggcaaa gcggttcaag
     1381 ctgatgtcgc tctgcattct cggcggcatc ctcggcatga tgccgttctg gaggccggag
     1441 ttcaatcctt cctatcatgc ccccggcgtg gtggtctcgc tgtcggcgat gcttctcacg
     1501 ctcgccctgg ttgcggtcat gcggagaggc ggttcaccgg taaaattcat gctgggattg
     1561 gccgcgcttt gcgtcgcact cgcttatgcg ggagcggtgt gagatatcgc cggggaacta
     1621 tcatggagaa aggcatagac cgatgaagga gaaagagctg ttgattgaaa ataagctggg
     1681 cctccacgcg agggctgcgg cgcagatcgt aaagaccgcc agcgcatata cttccaagat
     1741 ccttctcgcg aaagacggcc tggaggtcga cggcaagagc atcatgggaa tcatgatgct
     1801 tgcggccgca cagggcagca gcgtcaccgt ccaggcgcaa ggcgaagacg aagcagaggc
     1861 gatctctggg ctcgaacagc tcttcaagga caagttcggg gagaaagaat aagttcagag
     1921 ttcggagtgg aaacaggaaa ctaattcatg cacgagaaaa tctacagagg gatcgtggcg
     1981 tcgccgggga tcgtgatcgg gagggcgtat ctgctcgacc ggcgcaaggt ggtcgtcgcg
     2041 ggccgccgga tcgaggacgt cagcgtcaag gaagaggtcg cccggtttaa aaaggcggtg
     2101 gaactgtcca agacccagct cgaggacgtg aagaagcgga tcagcaaggg cctcggcaag
     2161 tcccacctct atatcctcga gacgcatatc atgctgctcg aagacaagat gctcgtcgag
     2221 ggcacgatca agcggatcag ggaatcgctt ctgaacgccg agggcgcgct caaggagacc
     2281 atcgctgcga tcggggccaa attcgacagc atcgaagacg aatacctccg cgaccggaaa
     2341 cacgatgtgg agcaggtggg cgaacgcgtg ctccgcaacc tcgtcggtca caagcaggag
     2401 agcctggcgg acatcaagga ggaggccatc atcatcgccc acgacctcgc gcccagcgac
     2461 accctcgtga tgcgcaagga caagatcctc gggttcgcga ccgatgcggg cagccggacc
     2521 tcccatacgg cgatcctggc gcgatcactg gggcttcccg ccgcggttgg tctcgagagc
     2581 atcacggcgt cggtgaagac cggcgacgtc gtgatccttg acgggatcca cggcgtggtg
     2641 atcgtcgatc ccgacgagga gacgtttctc gactatctca agaagcagag aaagtacaaa
     2701 tatttcgagc aggaactcga gaaactcaag tcgctgcctg ccgagaccct cgacggtcac
     2761 gtcgtgtacc ttcagggaaa catcgaactg cccgaggagg cgacctcgac ggcggagcac
     2821 ggcgggaccg gcatcggcct ctaccgtacg gagtttctct tcatgaaccg gcagacgctg
     2881 cccacggaag aagagcacta tgccgcctac cggcacgtgg cggaaagggc cacgccgcac
     2941 gaagtggtca ttcggactct ggacgtgggc ggggacaaga tcgatatggc cggagagttc
     3001 gagaaggagg ccaaccctgc cctggggctg cgggcgatca ggttctgtct gaagaagcgc
     3061 gacattttcc ggacccagct ccgcggcatc ctcagggcgt cggtcctcgg caacatcaag
     3121 atccttgtcc cgatgatctc cggcctgacg gagctctacg agacgaagca gatgatcgaa
     3181 gacgtaaagg cagaactccg ggccgagggc cggccgttca atgaggcggt gccggtcggc
     3241 gcgatgatcg agatcccgtc ggctgccatg atcgccgacc ttctcgcgcg gcaggtcgat
     3301 ttcttcagcg tcgggaccaa cgacctcatc cagtatgcct tggcgatcga ccggatcaac
     3361 gagcaggtgg cgtacctcta cgagccgctc cacccggcag tgttgcggat gctccgtacg
     3421 gtggtaagcg ccgcgcata
//
LOCUS       DASSLD010000471         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_629741,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000471 DASSLD010000000
VERSION     DASSLD010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629741"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /gene="trmD"
                     /locus_tag="VFK23_08490"
     CDS             <1..608
                     /gene="trmD"
                     /locus_tag="VFK23_08490"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET7319158.1"
                     /translation="TADDSPYGGGAGMVLKPEPIFKAFEVMKEGHPGEEFHTILLSPQ
                     GKVLDQRRAERLSEEQRRIVLLCGHYEAIDERVREALVDEELSIGDYVLTGGELAALV
                     VIDAAVRLLPGVLGDEESANRDSFGDGLLDHPHYTRPAEFRSRKVPEVLLSGNHAEID
                     KWRRREALRATLKKRPELLETAFLTDEDKQILDELKKELKG"
     gene            684..1079
                     /gene="rplS"
                     /locus_tag="VFK23_08495"
     CDS             684..1079
                     /gene="rplS"
                     /locus_tag="VFK23_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET7319159.1"
                     /translation="MNIIQSLEKAQVKEGIPSFRVGDTIRVHAKVVEGDKERIQVFEG
                     LVIGRQGSGAREVIRVRKLSYGVGVERLFPLHSPMIDKIELAKQGKVRRAKLYFLREL
                     RGKAARIKEKERVFATVAGATVTEPTEQK"
     gene            1223..1915
                     /locus_tag="VFK23_08500"
     CDS             1223..1915
                     /locus_tag="VFK23_08500"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET7319160.1"
                     /translation="MPHGRPRRKKPHPGRTDDLFGGELACDPLFFERRAREAGFTCIA
                     GLDEAGRGPLAGPVVAAAVVLPEGLLIPGLTDSKQVAEPDRERLFDVIREQALCYGVG
                     VADERTIDEVNILQATIIAMERALELLAPRPDYLLLDALTLPRVRLPQKPLIKGDYRS
                     HSIAAASILAKVTRDRIMLELHEKYPLYHFQNHKGYGTAEHLALLREHGPCEAHRKSF
                     NPVTQMLRRERP"
     gene            1912..>2026
                     /locus_tag="VFK23_08505"
     CDS             1912..>2026
                     /locus_tag="VFK23_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET7319161.1"
                     /translation="MNLFRKTLGQEGEDRAARYLAKQGYRILERNYRTPAGE"
BASE COUNT          439 a          604 c          624 g          359 t
ORIGIN      
        1 agaccgccga tgacagcccc tacggcggcg gggccgggat ggtgctgaaa cccgagccga
       61 tcttcaaggc ctttgaagtc atgaaggaag ggcacccggg agaggaattt cacacgatcc
      121 tgctctcccc gcagggcaag gtcctcgatc agcggcgggc ggaacgactc tccgaagaac
      181 agcgaaggat cgtcctgctc tgcggccact acgaggcgat cgacgagcgc gtccgcgagg
      241 cccttgtcga cgaagagctt tcgatcggag attacgtatt gaccggcggc gagcttgcgg
      301 cccttgtcgt gatcgacgcc gcagtacggc tcctgccggg ggttctcggc gacgaggagt
      361 cggcgaaccg tgattccttc ggcgacgggc ttctggacca cccgcactat acgcggcctg
      421 ccgagttccg cagcaggaag gttcccgagg tcctcctctc cgggaaccac gccgagatcg
      481 acaagtggcg ccgccgcgag gcgctcaggg ccacgctcaa aaagaggccg gagctgctgg
      541 aaactgcgtt cttgacggac gaggacaaac aaatactcga tgagttgaag aaagaattaa
      601 aaggatgaat cgtgcggcat gccgaatggt aactccgcat tccgcattcc gaattccgca
      661 ttttagtatc agggaggttt accatgaaca tcattcagtc tttggagaag gcccaagtga
      721 aggaagggat accgagcttc cgcgtggggg acaccatccg cgtccatgcg aaggtcgtgg
      781 aaggcgacaa ggagagaatc caggtattcg aaggcctggt gataggacgt cagggctccg
      841 gagcccgaga ggtcatccgc gtccgcaagc tgtcctacgg cgtgggcgtc gagaggctct
      901 ttccgctcca ttcacccatg atcgacaaga tcgagctcgc caagcaaggc aaggtccgcc
      961 gggccaagct ttatttcctg agagagctgc gcggcaaagc cgccaggatc aaggaaaagg
     1021 aacgcgtctt tgcgacagtc gccggggcaa cggtgacgga gccgacggaa cagaagtagt
     1081 tccactctta ctgtcatacc tgcgaaagca ggtatccatt ggcttctcta aaaataaact
     1141 tcaaaatgga ttcccgcttc cgcgggaatg ataaaaaatt caaagcagtg gtgtttggaa
     1201 tttctctttc tgtgaggttc ccatgcctca tggccgtccc cgccgcaaga aacctcatcc
     1261 gggtagaacc gacgatctct tcggcggcga actcgcgtgc gatccgctgt tctttgaacg
     1321 gcgggcgcgc gaggcgggat tcacctgcat cgcggggctg gacgaagccg gcagggggcc
     1381 gctagccggc ccggtggtgg cggcagccgt ggtgcttccc gaggggctgc tgatcccggg
     1441 gctcaccgac tccaagcagg tggcggaacc ggaccgggag cggctcttcg atgtgatccg
     1501 cgaacaggcg ctctgctacg gcgtcggcgt cgccgacgag cggaccatcg acgaggtgaa
     1561 catccttcag gcgaccatca tcgccatgga acgggcgctc gaacttctcg cgccaaggcc
     1621 cgattacctt cttcttgatg cgcttaccct tccccgcgtt cggctcccac aaaagcccct
     1681 catcaaaggc gattaccgga gtcatagcat tgccgctgcc tccatccttg ccaaggtgac
     1741 tagggaccgg atcatgctgg aactccacga gaaatatccc ctctaccatt ttcaaaatca
     1801 taaggggtat gggaccgcgg agcacctcgc gctccttcgc gagcacgggc cctgcgaagc
     1861 gcaccgcaag agcttcaatc ctgttacgca gatgctcagg cgggagcggc catgaacctc
     1921 ttccggaaga ccctcgggca ggaaggcgag gaccgcgccg cccggtacct ggcgaagcag
     1981 ggctacagaa tcctggagcg gaattaccgc accccggccg gcgaaa
//
LOCUS       DASSLD010000472         3101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_497742,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000472 DASSLD010000000
VERSION     DASSLD010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497742"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..1056)
                     /gene="ybgF"
                     /locus_tag="VFK23_08510"
     CDS             complement(193..1056)
                     /gene="ybgF"
                     /locus_tag="VFK23_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HET7319162.1"
                     /translation="MTKILASIITLLLVAACAPQAELVKTRSDMGGLREDVKALKARN
                     LELQKKLDQETQQVQNLQKRLDALDTKGGTDAQKAMADYGAKTDQIITDIQLLQGRLE
                     ENNFRLAELAQKSDDRAVKVSELAARVDEVDSKIKLLGAGAPAASAAAASPDKEKHSP
                     PKRIEPSEVYRQAKSDYDKGNFDLALAGFQHYMAQFPEASQADNAQYWIGECYYALKD
                     FNKAIEAFAKVMKNYPKSVKVPGAKLKTGLAYLNEKNPAKAKEYFHKVVKEHPGTNEA
                     EIAKDRLAKIK"
     gene            complement(1228..1803)
                     /gene="pal"
                     /locus_tag="VFK23_08515"
     CDS             complement(1228..1803)
                     /gene="pal"
                     /locus_tag="VFK23_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HET7319163.1"
                     /translation="MKSRRLYISILLLLGIFLLWGCPKKAEVARAPEAQPEVRQEAPA
                     VEAPKAEDAEAARRAAEEKAAQERAAAEARERAASAASGLKPIYFDFDKSFIRDDAKA
                     VMKANADWLKANPQAKVRIEGNCDERGTREYNQALGQRRASSAKKYLTDLGISAKRIS
                     LISYGKEKPVCTESDEACWQKNRRDEFVVAE"
     gene            2167..>3101
                     /locus_tag="VFK23_08520"
     CDS             2167..>3101
                     /locus_tag="VFK23_08520"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HET7319164.1"
                     /translation="MLLLRCVVIILSILILAPPAHADPTVFSGLAITRIDLKDDFGNP
                     WRRPGQVEELIGIKPGDILTGAAVREGISLLYLKGIFRDIIVEAFPDNNGVRLEYTFI
                     PITTVEKVLIHGSRRKLKGPVTEVLSPLIGKELHLDKLSGYRADILSLYEAAGYNNAA
                     VSFRIESGPVPDRESVQTGAGAAQPAPPITFNDELEPHKAILHVDIGEGEPTIIEKID
                     FSGNAAITEKDLIKAIKSRAGSPLRRDQLLDADKEAILKKYGDAGFPAAKVGPVDINF
                     RDHRAFVRITISEGPRVTVRFTGNRALKSKELKRS"
BASE COUNT          600 a          933 c          862 g          706 t
ORIGIN      
        1 gtctgaaatc cagcctgctt tttttattcc gaaatcagat cgggtataag ggaaggcagg
       61 ccctgtgcaa aagcgttgca ggcctgaaat ggtcaacgaa aaaccaagaa tgttcataaa
      121 aagagggggc cggtgtttta actccgggcc ccctctctca atgttgtatg acgtgaatat
      181 ttacttacat cgctacttta tctttgccag acggtctttc gcgatctcgg cctcgttcgt
      241 gccgggatgt tccttgacga ccttgtggaa atattccttg gctttggcgg gatttttctc
      301 gttcaaatag gccaggccgg tcttgagctt ggcgcccggg accttgacgc tttttggata
      361 gtttttcatg accttggcaa aggcctcgat cgccttgttg aagtccttca gggcataata
      421 acattcgccg atccagtact gcgcattgtc cgcctggcta gcttcgggaa actgggccat
      481 ataatgctgg aacccggcga gggcgaggtc gaagttgccc ttgtcgtaat cgcttttcgc
      541 ctggcgataa acttcggacg gctcgatcct cttgggcggg gagtgctttt ccttgtccgg
      601 ggacgcggcg gccgcggatg ccgccggagc gcccgcgccc aggagcttga tcttcgaatc
      661 gacttcgtcg accctcgccg ccagttcgga gaccttgacg gcccggtcgt cggatttctg
      721 cgcaagctcc gcgaggcgga agttgttctc ctcgagcctg ccctgaagaa gctggatgtc
      781 cgtaatgatc tggtcggtct tcgcgccgta gtcggccatc gccttctgag catctgtgcc
      841 gcccttggtg tcgagggcgt cgagacgctt ttgcaagttc tggacttgct gggtttcctg
      901 gtcgagcttc ttctgcagtt ccaggttccg cgccttcagc gccttgacgt cttcccgcag
      961 gccgcccatg tcggacctcg tcttgacgag ttccgcctgg ggagcgcatg ctgccacaag
     1021 caggagcgtg atgatcgatg cgagaatttt tgtcatcggc tgtcttttgg acactttgct
     1081 gccggatggt gtatgtgaat cggcgaaatg ggggagaaaa gaagaaccga aacggcgaaa
     1141 aggcgaacgg atgaaacgga aagacgccca gagctctttc gccgattctc cccgtcaccc
     1201 cctcgccgct tcaagtttct cccgagccta ctccgcgacg acgaattcgt cgcggcggtt
     1261 tttttgccag caggcttcgt cgctctcggt gcagacaggc ttttccttgc cgtagctgat
     1321 gagcgagatc cgcttggcgg atataccgag gtcggtaaga tacttcttgg cgctcgaggc
     1381 acgacgctgg ccgagggcct ggttgtactc cctcgttccc cgctcgtcac agttcccctc
     1441 gatcctgacc ttcgcctggg ggttggcctt gagccaatcg gcgttggcct tcatcactgc
     1501 ctttgcgtcg tcgcggataa acgacttgtc gaaatcaaaa tagatcggtt tgaggccgct
     1561 cgctgcggac gcggcccgct cccgggcctc cgccgccgcc ctctcctgcg cagctttctc
     1621 ctcggcggcc ctccttgcgg cctcagcgtc ctccgccttg ggggcctcga cggccggggc
     1681 ctcttgtcgg acctcgggct gggcttcagg cgctctggct acttccgcct ttttggggca
     1741 tccccacaac aggaagatgc cgagcaacaa caaaatagag atataaagac gtctgctctt
     1801 catgaatccg ccctcctctg cttccgcaca ttgaagtttt ttcatctgcc ggccggggca
     1861 cgttgcgcag ggctcggcaa gcagtgttca agatagcata cactcctgct cttgtcaaca
     1921 ggaacggaaa accttctctt cagaaggcac ccggtgcgct gtaacgtcag ccggcgggaa
     1981 ttaacagagg gcacgcagaa caaccggtga tgaaatagtc tcgatcagaa tcccgcctga
     2041 ttccgtactt tgcttctgca agaacctttc acctccgcgt tctctgcatc cacagcggtg
     2101 aaaccgactg tctcgcctct cgcaatcctt ctttacattc ccaccttgat gtgctaaaaa
     2161 tccttcatgc ttttgcttcg ttgcgtcgtc ataatcctct caattctcat tcttgcgccg
     2221 cccgcccatg ccgaccccac ggtcttctcg ggcctcgcga taacccggat tgacttaaag
     2281 gacgacttcg gcaatccctg gcgccggcct ggccaggtcg aggagctgat cgggatcaaa
     2341 ccgggagaca tcctcacagg cgccgcggtc cgggaaggca tatcgctgct ctatctcaag
     2401 gggatattca gggacatcat cgtagaggca tttccggata acaacggcgt gcggttggaa
     2461 tataccttca ttcccataac gaccgtggag aaggtcttga tccatggaag ccgccggaaa
     2521 ctgaaaggcc cggtcacgga agtgctttcc ccccttatag gaaaagagct ccacctggac
     2581 aagctttccg gataccgggc ggacatcctc tctctctacg aggcggccgg ttataataac
     2641 gcggccgttt ccttccgcat agagtccgga ccggttccag accgggaatc cgtgcagacc
     2701 ggagcgggcg cggcacagcc cgcgcctccc ataacattca acgacgagct tgagccgcac
     2761 aaggccatcc tccacgtcga catcggcgag ggcgaaccga cgatcatcga gaagatcgac
     2821 ttttccggca atgcggcgat aacggaaaag gacctgatta aggccatcaa gagccgcgcc
     2881 gggtcaccgc tccgccgcga tcaactcctc gacgccgaca aggaggcgat cctgaaaaaa
     2941 tacggcgacg cgggcttccc ggccgcgaag gtcggccccg tggacataaa cttccgcgat
     3001 catcgggcat tcgtacgcat caccatcagc gagggtcccc gggtaacggt ccggttcacg
     3061 ggtaaccgtg cgctcaagtc gaaagaactg aagcggagca t
//
LOCUS       DASSLD010000473         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1601140, whole genome shotgun sequence.
ACCESSION   DASSLD010000473 DASSLD010000000
VERSION     DASSLD010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601140"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2022)
                     /locus_tag="VFK23_08525"
     CDS             complement(<1..>2022)
                     /locus_tag="VFK23_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7319165.1"
                     /translation="LEQTDGVVLPLLKKLGVDPVRVKGDIEEALNRMPKVEGLVQTYL
                     SPRLGKLFERAEQEADRLKDEYVSTEHLLIAAADGDGAAREILARHGVSKDKIFTVLV
                     DIRGSQRVTDQNPEDKYQALTRYGRDLTELARRGKLDPVIGRDEEVRRVVQVLSRRTK
                     NNPVLIGEPGVGKTAIVEGLAQRIVSGDVPEGLKNKRVVALDMGALIAGAKYRGEFED
                     RLKAVLKEVTEASGEVILFIDELHTVVGAGAAEGAMDASNMLKPALARGELRCVGATT
                     INEYRKHIEKDPALERRFQPVLVREPSVEDTISILRGLKERYEVHHGVKIKDSALVSA
                     AVLSHRYIADRFLPDKAIDLIDEAASRLRMEIDSMPTELDEVERRTRQLEIEREAVRK
                     DQDPASRERLAKIDKEIAELSGKKSGLRAQWQAEKEGIQKIRAVKEQIEQARIEAEKA
                     ERSGNLGRAAELRYGRLPELQKQLESESAQLAAVQSQTKILKEEVDEEDVAEIVSKWT
                     GIPVSKMLEGEMKKLLHMEERLRMRVVGQDEAIAVVSAAVRRARAGIQDPNRPIGSFI
                     FMGPTGVGKTELARALAEFLFDDEQAMIRIDMSEYQERHTVSRLIGAPPGYVGYEEGG
                     QLTEAARRRPYSVILFDEIEKAHPEVFNVLLQLLDDGRLTDGQGRTVDFK"
BASE COUNT          322 a          690 c          554 g          456 t
ORIGIN      
        1 cttgaagtca actgttctcc cctgcccgtc cgtcaatcgg ccgtcatcga gcagctgcag
       61 cagtacatta aacacctcgg gatgcgcctt ttcgatctcg tcgaagagga tcaccgaata
      121 gggacgccgc ctcgccgcct cggtgagctg accgccctct tcatagccca catagcccgg
      181 aggcgcgccg ataagcctcg acacggtgtg gcgctcctgg tactcggaca tgtcgatacg
      241 gatcatcgcc tgttcgtcat cgaacagaaa ttccgcgagc gcccgggcaa gttcggtttt
      301 ccccacgccc gttggcccca tgaagatgaa actgccgatg ggccggttcg gatcctgaat
      361 ccccgcccgg gccctgcgca cggctgcgga caccacggcg atggcctcgt cctggcccac
      421 gacgcgcatg cggagacgtt cttccatgtg caacagcttc ttcatctcgc cttcgagcat
      481 cttggagaca gggatgcccg tccattttga aacgatctcc gcgacatctt cttcgtccac
      541 ttcttcctta agtatcttcg tctgagattg gaccgcggcg agctgcgccg actcgctttc
      601 gagctgcttc tgaagttcag gcagccggcc gtaccgcagt tccgccgccc ggccgaggtt
      661 gcccgaacgt tcggcttttt ccgcctcgat cctggcctgc tcgatctgct ccttgacggc
      721 ccgaatcttc tggatgcctt ccttctcggc ctgccattgc gcgcgaagac cgctcttttt
      781 tccggaaagt tccgctatct ctttgtcgat cttcgcaagc cgttcccgcg acgccgggtc
      841 ctgatccttt cttacggctt cgcgctcgat ttcgagctgc ctggtccggc gctcgacttc
      901 atcgagctcg gtgggcatgc tgtcgatctc catgcgcaga cgcgatgccg cttcgtcgat
      961 caggtcgata gccttgtccg gcaaaaaccg atctgctatg taccggtgtg agagaaccgc
     1021 tgcgctcacg agcgcggagt ccttgatctt gaccccgtgg tgcacttcgt accgctcctt
     1081 cagtccgcgc aggatcgaga tcgtgtcctc taccgacggt tcacgaacca gtacgggctg
     1141 gaacctccgc tcaagggccg ggtccttttc gatatgcttg cgatattcgt taatcgtcgt
     1201 cgcgcccacg cacctgagct cgccgcgggc aagcgccggc ttcagcatgt tcgatgcgtc
     1261 catggcccct tccgccgctc cggccccaac cacggtatga agctcgtcaa taaaaaggat
     1321 cacctcgccg ctcgcttcgg tcacttcctt cagaacggcc ttgagccggt cttcgaattc
     1381 gccgcggtac ttggccccgg cgatgagcgc acccatatca agggccacga cccgcttgtt
     1441 cttcagtcct tcagggacgt cgcccgagac tatccgctgc gcgaggccct cgacgatcgc
     1501 ggtcttgccc acgccgggct cgccgatcag gaccgggtta ttcttggtcc ggcgtgacag
     1561 gacctgcacc acgcgccgga cttcttcatc cctgccgatc acgggatcga gtttcccgcg
     1621 ccgggcgagt tcagtcaggt cgcgcccgta ccgcgtgagc gcctggtatt tatcttcggg
     1681 gttctggtcc gtcacgcgct ggctgcccct gatatcgacg aggacggtga aaatcttgtc
     1741 cttcgacacg ccgtgccgag caaggatctc ccgcgcggcg ccgtctccgt ctgcggcagc
     1801 gatcaaaagg tgttcggtgc ttacgtattc atccttcagc cggtccgcct cctgctccgc
     1861 gcgctcgaag agcttgccga gccgcggaga gagataggtc tgcactaggc cctcgacctt
     1921 cggcatcctg tttaaagctt cctctatatc ccccttgacg cgcacgggat cgactccgag
     1981 ttttttcagc aggggcagga cgacgccgtc cgtctgctcc aa
//
LOCUS       DASSLD010000474         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1210699, whole genome shotgun sequence.
ACCESSION   DASSLD010000474 DASSLD010000000
VERSION     DASSLD010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210699"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /gene="tilS"
                     /locus_tag="VFK23_08530"
     CDS             <1..1358
                     /gene="tilS"
                     /locus_tag="VFK23_08530"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET7319166.1"
                     /translation="RFGMLKPGERVLVAVSGGPDSVCLLSVLHDLSEELGVSLHVAHL
                     DHMFRGRESADESLFVARLAEKLGIPVTIEQFDVPAFCRERGLSSQDGAREVRYGFLH
                     RVAGTVGASRIATGHTAADQAETFLMRLLRGAGIAGLSAIPPVRENIIRPLIDVTRDE
                     VLEYLQSSGLEYVSDPSNTKPVYTRNRIRMEVLPVLKRFNPRIVETLASTAALLRDED
                     EAAEACLSANAAGMMMQEKNGVALSRDEFNALPQAYKRRMLRKAVHLAGSDERGLSSV
                     QVSDVIAFMAAAQTGRTMRIPYGLIIEREYEKFVICFEVHPEGFSYTLEMPGVTLIPD
                     LGMEVDAGIVNDIPAETSVPNYRWQALFDYDKIEPSLTMRNRRSGDWFCPTGMGGKSK
                     KLQDYFVDEKIPRHERDTVPILLSGNDVLWVVGLRTDNRFLPGKGTKRVLVVQVRREQ
                     R"
     gene            1503..2036
                     /gene="hpt"
                     /locus_tag="VFK23_08535"
     CDS             1503..2036
                     /gene="hpt"
                     /locus_tag="VFK23_08535"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET7319167.1"
                     /translation="MVGIDFGKPLFTREEIQRKIQELGTRISSDYAEKDLLVVGVLKG
                     AIFFMSDLLRALKISVRMDFIHCTSAKGSHPVQIIADIKEDIRGKEVLLVEDIMDSGV
                     TIDYLKKMLLERGPTSLKVCVLLDKTERRKVQIEADYAGFRIPNKYVVGYGLDYRDRY
                     RNLPYIAVLPLEERQAP"
BASE COUNT          519 a          541 c          640 g          507 t
ORIGIN      
        1 acagattcgg catgctgaag ccgggagaga gggtccttgt cgcggtttca ggcggtcctg
       61 attcggtctg tctgctttcc gtgctccatg atctttcgga agagctcggt gtaagcctcc
      121 atgttgcgca ccttgaccac atgttccgag gcagggagtc ggcggatgag tccctgttcg
      181 tggcgcgcct cgcggaaaag cttggcattc ccgtgaccat cgaacagttt gacgtgcccg
      241 ccttctgccg agaacgcggg ctgtcttccc aggacggggc acgtgaagtg agatacggtt
      301 ttttgcatcg ggtcgcggga acggtcggcg cttcccgtat cgcgacgggc catacggctg
      361 cggaccaggc agagaccttt cttatgaggc ttctgcgagg cgccgggatt gcgggcctct
      421 cggcaatccc gcccgtgcgg gagaacatca tccgtcccct gatcgatgtc acgagggatg
      481 aagtgctgga gtatctccaa tcgagcgggc ttgagtatgt gtccgacccg tcgaacacaa
      541 agccggtgta cacaaggaac cggattcgaa tggaggttct gcctgttctc aaacggttta
      601 atcctcggat tgttgaaacg ctcgcgtcta cggcggccct gctaagagac gaagatgaag
      661 ccgcagaggc ttgtttatca gcgaacgctg ccgggatgat gatgcaggaa aagaatggtg
      721 ttgctctgag cagagacgaa ttcaatgccc taccgcaagc atacaagcgc aggatgctta
      781 gaaaggctgt gcatctggca ggctctgacg aaagagggct gtcttcggtc caggtcagtg
      841 acgtgatcgc tttcatggcc gcggcccaga caggcaggac catgcgcata ccgtacggcc
      901 ttatcataga gcgcgagtat gagaaattcg tcatatgctt cgaagtgcac ccagaaggat
      961 tctcctatac acttgaaatg cccggcgtca ccttgattcc cgatctcggc atggaggtcg
     1021 atgcagggat tgtcaatgac atcccggctg aaacgagcgt tccaaattac cgctggcagg
     1081 cactgttcga ttatgataaa atagagccgt cgttaacgat gagaaacagg cgctcgggcg
     1141 actggttctg tcccacaggt atgggaggga agagcaagaa gcttcaggac tacttcgtgg
     1201 acgagaagat cccgagacat gagcgagata ctgtccccat cctcctctcg ggaaatgacg
     1261 ttctctgggt cgtgggtctc cgaacggaca accggttcct gccggggaag gggacgaaga
     1321 gagtcttggt ggttcaagtg cgacgtgaac aaagataggg gtaatggccc agggaaagaa
     1381 gcggccactt gagggtctgg gtcagctgaa ttaatgatgt attaaccctt atccctaacg
     1441 actcgaacct cacgaagata gtttaatctg taccctaatt tattttccat ggagggcatg
     1501 caatggtagg tattgatttc gggaagccgc tctttacccg tgaggagatt cagcggaaga
     1561 tccaggaact cggcaccaga atatcatctg attacgcgga gaaagacctg ctcgtagtcg
     1621 gggtgctcaa gggagctatc tttttcatgt ccgaccttct ccgggcgctt aagatctccg
     1681 ttcgtatgga ttttattcac tgcaccagcg ccaaaggcag ccatcccgtc cagatcattg
     1741 ctgacatcaa ggaagacatc agaggtaaag aagttctgct tgttgaggac ataatggatt
     1801 cgggtgtgac catcgattac ttgaagaaaa tgctccttga acgcggcccc acttcgctca
     1861 aggtttgtgt gctgctggac aagactgaac ggcggaaggt ccagattgag gcggactacg
     1921 caggtttccg tatcccgaac aagtacgtcg ttggctacgg cctggactac cgggaccgct
     1981 atcggaatct gccgtacatc gcagtgctgc ccctagaaga gcgtcaggca ccgtaatgac
     2041 gataactata tatcacacag cttgacagga tatcagcagg ttctgcctgt aatttaaagc
     2101 gacaagcaag tcatttgata aacggatgtt ttggggcagg gatgattgcg gatcccctga
     2161 aagcgggata aaactattgt ttctctgcaa ataatgtgtt attattt
//
LOCUS       DASSLD010000475         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_526564,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000475 DASSLD010000000
VERSION     DASSLD010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526564"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VFK23_08540"
     CDS             complement(<1..360)
                     /locus_tag="VFK23_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319168.1"
                     /translation="MNTRKTLLGSALVLYFVIAFEVLIMISPFAGFFYSVFNPVLLKL
                     AAYPSTRWLSAFYLPHMILPQDGLLQFVRVMGSVLFVLGMAIFFVCATQIYTAKFAKK
                     GAVVGGLYSFIRHPQYLA"
     gene            complement(549..875)
                     /locus_tag="VFK23_08545"
     CDS             complement(549..875)
                     /locus_tag="VFK23_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319169.1"
                     /translation="MPFKFRPIILLLALALLIQNTCPFGAAGKSTVSHACGHCQMKHA
                     VTASPIGQKQLVSDSSSVHFPLYVFAVPKTVHTFQLSPVKSARPVLADFYQDAQPDGL
                     LRPPQA"
     gene            complement(937..1623)
                     /locus_tag="VFK23_08550"
     CDS             complement(937..1623)
                     /locus_tag="VFK23_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET7319170.1"
                     /translation="MKAHILQHVLFEDIGSMASWLDERGAEVSYTRFFEGAALPQMKN
                     LDLVIAMGGPMSVNDESSLPWLRPEKQFIRDIAEKGVPVLGVCLGAQLIASAHGARVY
                     RNRYKEIGWYPIEATPGPADTFRFPEKCTVFHWHGETFDLPSGAVRLAKSAACENQAF
                     QIGRHVIGLQFHLETTPKSAKAILDNCADELVPGPYVQTASEMRAAKDTAYVEINGLM
                     AGVLSYLTRS"
     gene            1925..>2740
                     /locus_tag="VFK23_08555"
     CDS             1925..>2740
                     /locus_tag="VFK23_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7319171.1"
                     /translation="MNDESMQQFQDFMRLAGGFRASRVILTANNLEVFEHLKTPKTAV
                     EIATKLGTDQRATEILLDAVASLGLLRKTGVKYRNAAPAKKFLLKESLWYQGDMLRHF
                     DALWKSWSGLDEVVRTGLPNRAGGRDYEVFIRAMHNNAAFRAKSVVSSIDLRGVKKAL
                     DLGGGPGTYSMELAGRGVSVTLFDLPDTLAVAKKIIREEKGWNIDFLSGDYYSDDIGK
                     GYDLVFISQIFHSMSEENCLTLLEKSRNALNLNGRIAVHEFYLEKDRAHPVPGA"
BASE COUNT          648 a          710 c          786 g          596 t
ORIGIN      
        1 cgcgagatac tgggggtggc ggataaacga gtacagcccg cccacgaccg cacccttttt
       61 cgcaaacttg gccgtataga tctgcgtggc gcagacgaaa aagatcgcca tgccgagaac
      121 aaacaggacc gagcccatga cgcgcacgaa ctgcaacagc ccgtcctgcg gtaatatcat
      181 gtggggcagg tagaacgcgc tcagccaccg tgtcgacgga taagccgcga gtttgagcag
      241 gacgggatta aagaccgagt agaagaagcc cgcaaagggg ctgatcatga tcagcacttc
      301 aaaggcgatg acgaaataga gcacaagcgc gctcccgagg agcgtctttc tggtattcat
      361 aagaaacctc catcaatagt atataccacg gagccacgga gagatgaaaa cgacggacga
      421 tagacgatag acgagggacg agggacgatt cgtaccggga atatggcagt cctcgtcccc
      481 cgttttcgaa tataaggacg ttctctgtgc tctctgtggt gataattatg gattctggct
      541 tttgcgggtt acgcttgggg aggtctcagg agtccgtccg gctgcgcgtc ctggtaaaaa
      601 tccgcaagga ccgggcgcgc ggacttgacg ggactgagct ggaatgtgtg gaccgtcttc
      661 ggaacggcga agacgtagag cgggaaatgg acggatgagg agtcggaaac gagttgtttt
      721 tgacctatgg gagaagcagt cacggcgtgt ttcatctggc agtgcccgca cgcgtgggac
      781 acggtggact ttccggcagc gccgaatgga caggtgttct ggatcaggag cgccaaagca
      841 aggagcagta tgatcggcct gaacttaaac ggcatgtcgc tatgatagga tcttgtgcgg
      901 atgatgtcaa acgcttttta gatatttctc acaggtttaa gatcgtgtga gataggaaag
      961 cacccctgcc ataaggccgt tgatctccac gtacgcagtg tccttggcag cccgcatttc
     1021 ggatgcagtc tgtacatagg gccccgggac cagttcgtcg gcgcagttat ccaggatggc
     1081 tttggcgctc ttgggagttg tttcgagatg gaactgcagg ccgatgacat gccgccctat
     1141 ctgaaatgcc tggttctcgc atgcagcgct cttcgccagc cggaccgccc cggagggaag
     1201 atcgaacgtc tcgccgtgcc agtggaaaac cgtgcatttt tcagggaagc gaaatgtatc
     1261 cgccgggccc ggcgtcgcct cgatcggata ccacccgatc tccttgtaac gattcctgta
     1321 gaccctcgct ccatgcgcgc tcgcgatgag ttgagccccc aggcagacac cgagcaccgg
     1381 cacgcctttc tccgctatgt cgcgaatgaa ctgcttttcc gggcgaagcc acggcaggga
     1441 cgattcatcg ttgacgctca tgggccctcc catggcgatg acgagatcga ggttcttcat
     1501 ctgcgggaga gccgcgccct cgaaaaaccg ggtgtagctc acctctgccc cgcgctcatc
     1561 cagccacgaa gccatgctgc cgatatcctc aaaaaggaca tgctgtaaga tgtgtgcttt
     1621 catccaagtg cctcccccac gggcggagat cgattgctaa aatggtttca agaaatcctt
     1681 cagatattat tgacgataac ttcggcgatt tcaagtcatt tcagtttccg gttcattgac
     1741 atttcattct gttttacgat atgatgaaca tgccccattt gatgtattca gagtgctgta
     1801 ataagttatc ttgtcattcc cgccaaagcg ggaatccatt ttgaagtttt catccgaaaa
     1861 ggccattgga tgcctgcttt tgcaggcatg acaggcggga tgaaatcatg aagggacctt
     1921 tttcatgaac gacgaatcta tgcagcagtt ccaggatttt atgcgtcttg ctggagggtt
     1981 ccgcgcttcg cgcgtcattt tgacggcgaa caacctcgag gtctttgaac atctgaagac
     2041 gccgaagacc gccgttgaaa tcgcgacgaa gctcggtacg gaccagcggg caacggagat
     2101 acttctcgac gccgtcgcgt ccctgggtct gctcagaaag accggggtga agtatcgaaa
     2161 tgcagcgcct gcgaagaagt ttcttctgaa agagagcctc tggtaccagg gcgatatgct
     2221 gcggcacttt gacgccttat ggaagagctg gtcgggcctc gacgaggtcg tgagaacggg
     2281 cctgccgaac cgtgccggag gacgggacta cgaggtattc atcagggcaa tgcacaataa
     2341 cgcggccttt cgggcgaaga gcgtcgtctc atccatcgat cttcgcggcg tcaaaaaggc
     2401 gctcgacctc ggcggcgggc cgggaactta cagcatggaa cttgccgggc gcggagtctc
     2461 agtcacgctc tttgaccttc cggacaccct tgctgttgca aaaaagataa ttagagaaga
     2521 aaaaggctgg aacatcgact tcttgagcgg tgattattat tccgatgaca ttggaaaggg
     2581 atatgacctg gtattcatca gccagatatt tcactccatg tccgaagaaa actgtctcac
     2641 acttcttgaa aaatccagga atgcgctgaa ccttaacggc aggatcgcgg tccacgaatt
     2701 ttacctggaa aaggaccggg cacaccccgt ccccggtgcg
//
LOCUS       DASSLD010000476         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_590865,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000476 DASSLD010000000
VERSION     DASSLD010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590865"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..332)
                     /locus_tag="VFK23_08560"
     CDS             complement(60..332)
                     /locus_tag="VFK23_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319172.1"
                     /translation="MHTDEFEISLHRELKVCENTIQRIRKTLGMMEQKHRKTTATFIE
                     EYRNGKLDVEKEQEDDYRVWESSYESLRKWQDLEQQYREQFRKMKV"
     gene            454..981
                     /locus_tag="VFK23_08565"
     CDS             454..981
                     /locus_tag="VFK23_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET7319173.1"
                     /translation="MVLFGVAAGRVRRSGFMKLSTKGKYGVRAVFEIARRYGKGPITI
                     KEIADRQGISFSYLEQILHKLGKAGVIESVRGPAGGYLLARKPSDLTIGDIVRTLEGP
                     IALSHCLEPGESEECSQTDACVARMVWAQVGAKIEEALDSITFDDLLQQQPEQKEPIL
                     LKHGRKAAMTGKGVC"
     gene            983..2170
                     /gene="nifS"
                     /locus_tag="VFK23_08570"
     CDS             983..2170
                     /gene="nifS"
                     /locus_tag="VFK23_08570"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HET7319174.1"
                     /translation="MKKVYLDHAATTPVHPKVLEAMLPFFSDKFGNPSNLHDIGREAK
                     NAVEEARAKTAALLGARPEEIYFTSSGAEANNFALKGIVQANSQKGRHIIVSQIEHFS
                     ILHPVKTLEKAGFTVTYLPTDKQGLVDPDAVANAITKETVLVSIMHANNEIGTIEPIE
                     EISKITKERGVLFHTDAVATVGWIPVNVQTLGVDALSLSGHQFYGPKGAAALYVRKGV
                     RIKPQIEGGIQEDGRRAGTENVPALVGLGKAAELAAAEVLHRMGYVAPLRDRLQKGLM
                     EKIDHLVINGHPTRRLPHNLNVSMWYVEGESMLLFLNMEGISVSSGSACTSRSLKSSH
                     VLACIGTDAAVANGTLLLTLGMGTTAEDIDYVVEKLPPIVQRLREMSPLYEDMIKKEA
                     ARK"
     gene            2217..>2306
                     /locus_tag="VFK23_08575"
     CDS             2217..>2306
                     /locus_tag="VFK23_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HET7319175.1"
                     /translation="MEQYSSKVMDHFAPPHNVGEIENADGVGTV"
BASE COUNT          511 a          652 c          697 g          446 t
ORIGIN      
        1 cccctgactt ataactctcc tgctttcctc cgcgcccccg cgtctccgtg gcaaaaatat
       61 taaactttca tcttccggaa ctgctcccgg tactgctgct ccaggtcctg ccatttcctg
      121 agggattcat agctgctttc ccagacccgg tagtcgtctt cctgttcctt ttctacgtcc
      181 agcttcccgt tgcggtattc ctcgataaag gttgccgtgg tcttgcgatg tttctgctcc
      241 atcatgccga gggttttcct gatcctctgg atcgtattct cgcagacctt caattcccgg
      301 tggagtgata tctcgaattc gtcggtatgc ataccgtacc tccgtggccc ttctatacca
      361 tagtataaca atgcagtgaa gacaagccaa aaatttattc ttgacagaat tagtcaagtt
      421 tgttatcgtt agggtaacga acgcaggacg aaaatggtcc tgtttggcgt tgccgccgga
      481 cgagtgcggc ggagcgggtt catgaagctt tccacgaagg ggaaatacgg cgtcagggca
      541 gtcttcgaga ttgcgcggcg ctacggtaag gggccgatca cgatcaagga gatcgcggac
      601 cgccaaggga tttccttctc ctatctcgag cagatcctgc acaagctggg gaaggcgggc
      661 gtgatcgaga gcgtgcgggg acctgcaggc ggatacctgc tcgcgcgcaa gccgtcggac
      721 cttaccatcg gagacatcgt gcggactctg gaggggccta tcgccctctc acactgtctc
      781 gaacccggag aatcggaaga atgctcccag acggacgcct gcgttgcgcg gatggtgtgg
      841 gcgcaggtgg gggcgaaaat cgaagaggcc ctcgacagca tcacctttga cgatcttttg
      901 cagcagcagc cggaacagaa agagcccatc cttctgaaac acgggaggaa ggccgccatg
      961 acagggaagg gggtatgtta gcgtgaaaaa agtctatctc gatcatgcag cgacgacgcc
     1021 ggtacatccg aaggtgctgg aggccatgct gcccttcttc tcggacaagt tcggcaaccc
     1081 gtccaacctg cacgacatcg gcagggaggc gaaaaacgcc gtcgaagagg caagggccaa
     1141 gaccgctgcg ctcctcgggg caagacccga ggaaatctat tttacctcga gcggcgcgga
     1201 agccaacaac tttgcgctca agggaatcgt ccaagcaaac agccagaagg gcagacatat
     1261 catcgtatcg cagatcgagc atttttcaat tcttcatccg gtaaagacgc tcgagaaggc
     1321 gggtttcacg gtcacgtacc tcccgacgga caaacagggg cttgtggacc ccgatgcggt
     1381 cgccaatgcg atcaccaagg agacggtcct cgtctcgatc atgcacgcga acaatgaaat
     1441 cggcacgatc gagccgatcg aggagatttc gaagataacg aaggagcggg gcgtgctctt
     1501 ccataccgac gccgtggcga ccgtcggctg gatcccggtg aatgtccaga ctctcggggt
     1561 ggacgcgcta tcgctctcag gccaccagtt ctacggccca aagggggccg ctgcgctgta
     1621 cgtgcggaaa ggggtccgga tcaaaccgca gatcgagggc ggcatccagg aggacggcag
     1681 gcgagcgggc accgagaacg tgccggccct cgtggggctg ggcaaggccg cggagcttgc
     1741 cgcggcggaa gtcctgcacc ggatgggcta cgtcgcgccg ctccgggacc ggctgcagaa
     1801 ggggctcatg gaaaagatcg accatctggt catcaacggc cacccgacgc ggcggctgcc
     1861 gcacaatctg aatgtctcca tgtggtatgt cgagggagaa tcgatgcttc tgttcctgaa
     1921 catggagggg atctcggtct cgagcggctc ggcgtgcacg tcgcgctcgc ttaaatcgtc
     1981 gcatgtgctt gcgtgcatcg ggaccgatgc cgcagtcgcc aacgggacgc tgctcctgac
     2041 gctcggcatg ggaactaccg cggaagacat agactacgta gtagagaagc tgcctcccat
     2101 cgttcagcgg ctgcgggaga tgtcgcctct ctacgaagac atgatcaaga aggaagcggc
     2161 aaggaaataa aaccagcagc ggcgggccca agcccgcggc tacaacggag gatcatatgg
     2221 aacagtacag ttcgaaggta atggatcatt tcgccccccc gcacaatgtg ggagagatcg
     2281 agaacgccga cggcgtaggc accgtg
//
LOCUS       DASSLD010000477         3175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_264141,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000477 DASSLD010000000
VERSION     DASSLD010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264141"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..1476
                     /locus_tag="VFK23_08580"
     CDS             340..1476
                     /locus_tag="VFK23_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HET7319176.1"
                     /translation="MQKKYLLAPGPTPVPPEALLAMAMPIIHHRAPDFLPVLDSAKKG
                     LQWLYQTKNDVLILCSTGTGGMVGSVNNFLSPGDDVLVVNGGKFGERWTKICQAYGMK
                     VEELVVEWGYAAKPEQVEAALRKNPKIKAVFVQANETSTGVYHDIKSLAAVVKKTDAL
                     FVVDAISALVAHDIRTDEWGIDVMIGGSQKGVMLPPGLAFVSVSDKAWKMADAAKTPK
                     FYFNFKKERENLAKNQTNFTSAVTLIIGLNACIQMLRDEGLEKVFARHDRLARATRAA
                     AGALGLKLFPKESPSNALTAIEAPAGVDGQAIYKNLREKYGITGAGGQDKLKGKIFRI
                     AHLGYADTFDVITAVAGIEMVLKGLGHPVKLGTGVAAAQEILMK"
     gene            1634..>3175
                     /gene="serA"
                     /locus_tag="VFK23_08585"
     CDS             1634..>3175
                     /gene="serA"
                     /locus_tag="VFK23_08585"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HET7319177.1"
                     /translation="MKVLISDNLSPVGVEILKKAGLDVDARSKTSVEEIEKIIGDYDA
                     LIIRSATKVTAGLLEKAAKLKVVGRAGSGLDNVDIPAATKKGIVVMNTPGGNTVTTAE
                     HTIGMIFACARMIPQAYASLKAGKWEKKKFEGVELYDKTLGVVGLGAIGGVVASRAIG
                     LGMKVLAYDPFISTEKAKSLGIELGDLPAIYRRSDFITIHTPKTKETANLINKDTIAQ
                     MKDGVRIINCARGGIVNEQDLYAALKSGKVAAAAFDVFEKEPPENHPLLSLDNFIATP
                     HLGASTLEAQENVAVAVAEQVVDYLVAGTVRNAVNVPSVPADQLPTLSPYINLAERMG
                     LFIAQLSEGGLTKVTVEYSGEVANLKQEPITLSALKGLLTPTLQENVNYVNAPLIAKD
                     RGIEVKVSRSSGATEYTSLVTLRIKAGGKDVSASGTLNSKKEPRIIQVDNFPMETVPE
                     GDMLVLMNNDKPGVIGGIGTLLGQNGVNIARMQFGREKQGGLAMSVVSIDSVVSDELM
                     GKIRKL"
BASE COUNT          729 a          934 c          960 g          552 t
ORIGIN      
        1 atcctgaggg tgcactgcaa cggtgatcag ttcggatcgc ctggagccag gtgcaacaag
       61 ccagatcaaa gcaagcgtca ataccgccgg aagaaaggga cgtcttatca agcactttgt
      121 cgtttattcc aatgatctgt gaatgagaaa aagagaggca gcatcccttg aaacatcaca
      181 gacacccgca atatcccagc gatacgccaa ccccgggcat aggtcccgcc ccgacgggac
      241 tataatcttc ttcagatagc acgatttcgc tatcacgctt aaaccaaaca acaaacccgc
      301 aggcgtttca gcttgccctg ttcaatggag gacgcagcta tgcaaaaaaa atatctcttg
      361 gcccccggcc cgactcctgt gccccctgag gcccttctgg caatggccat gccgatcatc
      421 catcaccggg cgccggattt ccttcccgtg ctcgattccg cgaaaaaagg ccttcaatgg
      481 ctgtaccaga ccaaaaacga cgtgctcatc ctctgttcca ccggcacggg aggcatggtc
      541 ggttcggtaa ataattttct cagcccgggt gacgacgtgc tcgtcgtgaa cggcggcaag
      601 ttcggcgaac gctggaccaa gatctgccag gcctacggca tgaaggtcga ggaactggtc
      661 gtggagtggg gctatgcggc aaagcccgag caggtcgagg ccgcgctcag gaagaatccg
      721 aagatcaaag cggttttcgt ccaggcgaac gagacgtcga ccggcgtcta ccatgacatc
      781 aaatcacttg ccgcagtcgt gaagaagacc gacgcgcttt ttgtcgtgga cgcgatctcg
      841 gccctcgtcg cgcatgacat caggaccgac gagtggggca tcgacgtcat gatcggcggc
      901 tcgcagaaag gcgttatgct gccgccgggt ctcgccttcg tgtccgtgtc cgacaaggcc
      961 tggaagatgg ccgatgcggc caaaacgccg aaattctatt tcaatttcaa gaaggagcgc
     1021 gagaaccttg ccaagaacca gacgaatttc acctcggcgg tgacgctcat catcgggctc
     1081 aacgcctgca tccagatgct gcgggacgaa ggcctcgaga aggtgtttgc acgccacgac
     1141 cggctcgcca gggccacccg cgcggctgcg ggggcgctcg gcctcaagct cttcccgaaa
     1201 gaatccccgt cgaacgcact cacggccatc gaggctccgg cgggcgtgga tgggcaggcg
     1261 atctacaaga acctgaggga aaagtacggc atcaccggcg caggaggcca ggacaagctc
     1321 aagggaaaga tcttccgcat cgcgcatctg ggatatgccg acaccttcga cgtaataacg
     1381 gccgtggcgg ggatcgagat ggtcctcaaa ggcctgggcc accccgtcaa actcgggacc
     1441 ggcgtcgccg cggcccagga aatattgatg aaataaagaa tagactttta ccacagaggc
     1501 gctgagacgt agagaaaaga gaaaccaaga caaaagcgct tttctttgcg ggctttgcgt
     1561 ctttgcgaga gatgattcta gacgttctct gtgcctctgc gtctctgcgg ttaatttttg
     1621 ttttggagga atcatgaagg ttctcatcag cgataatctc tcccccgtcg gcgtggaaat
     1681 attgaagaag gccgggctcg acgtcgacgc ccggagcaag acctcggtcg aagaaatcga
     1741 gaagatcatc ggcgactacg acgcgctcat aatccgctcg gcgaccaagg tcacggcggg
     1801 gcttttggag aaagcggcga aactcaaggt cgtgggccgc gcgggcagcg gcctggacaa
     1861 tgtggacata ccggctgcca caaagaaggg catcgtggtc atgaacacgc cgggcggcaa
     1921 tacggtcacg accgcggaac acacgatcgg catgatcttc gcgtgcgccc gcatgatccc
     1981 ccaggcctat gcctctctca aggcgggcaa gtgggaaaag aaaaagttcg agggcgtcga
     2041 gctttatgac aagaccctcg gcgtggtcgg cctcggcgcc atcggcggcg tggtcgcgag
     2101 ccgggccatc gggctcggga tgaaggtcct ggcctacgat cccttcatct cgacggaaaa
     2161 agcgaaatcc ctcggcatcg agctgggcga cctgccggcg atctaccggc ggtccgattt
     2221 tatcacgatc catacgccca agacaaagga aacggcgaac ctcatcaaca aggacaccat
     2281 cgcccagatg aaagacggcg tgcgcatcat caactgcgcc cggggcggaa tcgtgaacga
     2341 gcaggacctc tatgcggcct tgaagagcgg caaggtggcg gctgccgcct tcgacgtgtt
     2401 cgagaaggag ccgccggaaa accatccgct cctctcgctg gacaacttca tcgccacgcc
     2461 gcacctcggc gcctctaccc tggaggcgca ggagaatgtg gccgttgccg tggccgagca
     2521 ggttgtggac tatctcgtcg cggggactgt ccggaacgcg gtgaacgtgc cgtcggtgcc
     2581 ggcggaccag cttccgacgc tctctcccta catcaacctc gcggagcgca tgggcctgtt
     2641 catcgcccag ctctccgaag gcggcctcac gaaggttacc gtggagtaca gcggggaggt
     2701 ggcgaacctc aaacaggagc cgatcaccct ctcggcgctc aagggcctgc tcaccccgac
     2761 cttgcaggaa aacgtgaact acgtgaacgc gccgctcatc gccaaggacc ggggcatcga
     2821 ggtgaaggta tcccggtcca gcggggcaac ggagtacacg agcctcgtga cgctcaggat
     2881 caaggcaggt ggcaaggacg tgtccgcgtc gggtacgctc aacagcaaaa aggagccgcg
     2941 catcatccag gtggacaatt tcccgatgga gacggttccc gagggcgata tgctggtgct
     3001 catgaacaac gacaagcccg gcgtgatcgg cggcatcggg acgctcttgg gccagaacgg
     3061 cgtaaacatc gcgcgcatgc agttcggccg cgagaagcag ggcggcctgg ccatgtcggt
     3121 ggtcagtatc gacagcgtgg tgtccgacga actcatgggc aagatcagga aactg
//
LOCUS       DASSLD010000478         2926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_463529,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000478 DASSLD010000000
VERSION     DASSLD010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463529"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..516
                     /locus_tag="VFK23_08590"
     CDS             67..516
                     /locus_tag="VFK23_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7319178.1"
                     /translation="MKKRAARAVFFGLVLLAFPVLGAETQQDRNKRTVVEFYEKAINQ
                     KDFEAASKYMGPRYTQHNPLAADGPEGLRAYLQYLRATYPNSHSEIKRVFADGDYVIL
                     HVHAVRAPGTRGSAIVDIFKLEGGKVVEHWDVIQEIPEKAANSNGMF"
     gene            856..1392
                     /locus_tag="VFK23_08595"
     CDS             856..1392
                     /locus_tag="VFK23_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319179.1"
                     /translation="MHTTTVASWEYQNDFFSIVIRESGQRPGSYVFSCGLSLPRFNPI
                     IWGRTGICSNAAFKALQQLRADVSYFALQRGIVTRNIETRSFPAAEKGDGWYQENDLL
                     IEDASPEVVREILEFSARDLVQTILTASRPDVTVPVGSQAPEAMQRFLESLGIPNYRE
                     AAPSPILKPTGETVDGRR"
     gene            complement(1580..2152)
                     /locus_tag="VFK23_08600"
     CDS             complement(1580..2152)
                     /locus_tag="VFK23_08600"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET7319180.1"
                     /translation="MRKNVKRCPWPSDDPLMIAYHDTEWGVPLHDDRKLYEFIVLDGA
                     QAGLSWSTVLRKRENYRRAFHGFDPGIVARFTAKDVERLLKDTGIIRNRLKIESAITN
                     AKMFLAIRKEFGSFDAYIWQFTGGKTVQHRCRRLADIPTTSKESDAMSKDLKKGGFKF
                     VGSTICYAFMQAAGMVNDHLVDCFRHREVQ"
     gene            complement(2256..>2926)
                     /locus_tag="VFK23_08605"
     CDS             complement(2256..>2926)
                     /locus_tag="VFK23_08605"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HET7319181.1"
                     /translation="FHAGSLVAVVSQGLILGGILSGTKVSGENFSGGAFDWLSPFSIA
                     LAAGLIAGYVLLASTYLIIKTAGPVQNGAYRKALRAALVVLIFQVLMIAWTPFQYPLV
                     LTHWLTPPRFYFIWIFPVLGMASFYGIISSLRSRREIAPFIFSSVFFFAGYLGLFASI
                     YPYAIPPEITFQEAAAQRETLLFTLSGAVFILPVILGYTVYSYRVFRGKVGKEGSADR
                     YIAS"
BASE COUNT          713 a          812 c          792 g          609 t
ORIGIN      
        1 tgggacaacc aggagttcat gcgccagatc gggctggcca aatagcacca gcgaaaggaa
       61 gtcgatatga agaaaagagc ggcgagagcg gtgttcttcg gcctggtatt attggccttc
      121 ccggtcctgg gggctgaaac gcagcaggac cggaacaagc ggaccgtggt tgagttctac
      181 gagaaggcga tcaatcagaa ggatttcgag gcggcctcca aatatatggg gccgcgttac
      241 actcagcaca acccgctggc tgcagacggc cccgagggtt tgcgcgcgta tcttcagtac
      301 cttcgggcaa cgtacccgaa ttcacacagc gagatcaagc gcgtattcgc tgacggggac
      361 tacgtgatcc tgcacgtgca cgcggtgcgc gcacccggca cgcgcggcag cgctatcgta
      421 gacatcttca agctcgaagg cggcaaggtc gtggagcatt gggatgtcat ccaggagata
      481 ccggagaagg ccgccaactc gaacgggatg ttttgacgca gggccctgtc tcgacccgga
      541 caagtcggaa ttaaaaccgg tctcgggcaa agccgcaggg acgcggagaa agaaaatccg
      601 ctagggataa aaaaacccgg tctatcgcat ttctctgcgc cttcgcgtct cagctcgaaa
      661 tattctgccg ttttgtgacg ggccctcacc tgcacactga gcgggatgat cgcaggcgat
      721 tagcaggaga tatcaaatca taacttcaga ttaaaattat tctaatttac ttgaatccgg
      781 caacgccgtg ggttatactg aggacaaata ggataaattc tgtacagttt cgtcccccaa
      841 aggaggcagc caatcatgca taccacgacc gtcgcatcat gggagtacca gaatgatttc
      901 ttctctatcg tcatcagaga atccgggcag cggcccggga gctacgtctt ttcctgcggc
      961 ctcagcctgc ccaggttcaa ccccatcatt tggggaagga ccggcatctg ctccaacgcg
     1021 gcattcaagg ccttgcagca gcttcgcgcc gatgtgtcct acttcgccct tcagcggggc
     1081 attgtaacaa ggaacatcga aacccgttcc ttcccggcgg ctgaaaaggg ggatgggtgg
     1141 tatcaggaaa acgatctcct gatcgaggac gcgtctccgg aggtggtccg ggagatcctc
     1201 gagttcagcg cgcgggacct cgtccagacg atccttactg ccagccgtcc ggatgtgacg
     1261 gtccctgtcg gctcccaggc tcccgaagca atgcagcgct tccttgaatc cctgggcatc
     1321 ccgaattacc gggaagcggc cccatcaccg attttgaaac cgaccggaga gacagtagac
     1381 ggcagacggt agaagaaaca gagacggcga aaacagaatg gcccgagatc gttctcgggc
     1441 cttttttatt tcattggcgg tacgctatta agagtatggt tttacgagct cggctgagcg
     1501 ctgtctccat ctacattatt ccccactaag ctgatgtaat gcgagaaaaa acagtctgag
     1561 aaacatcttc ctcaggtcgt tactgcactt cccggtgtct aaagcaatcc acgagatgat
     1621 cattgaccat ccctgccgcc tgcatgaacg catagcagat cgtggagccc acgaacttga
     1681 atcccccctt cttcaagtcc ttgctcatgg cgtctgattc cttggacgtt gtcgggatat
     1741 cggcaagccg cctgcaccgg tgctgcacag tctttccgcc ggtgaactgc cagatatagg
     1801 catcgaagga cccgaactct ttccggattg cgaggaacat cttcgcattg gtgatcgccg
     1861 attcgatctt cagccgattg cggatgatgc ccgtgtcttt cagcagccgc tccacgtcct
     1921 ttgccgtgaa ccgggccacg atcccgggat cgaatccgtg gaaagccctg cggtagttct
     1981 cccgctttct gagcaccgtt gaccacgaga gccccgcctg cgcgccgtcg agcacgatga
     2041 actcatacag tttccgatcg tcgtgaagcg gcacgcccca ttccgtgtca tgataggcaa
     2101 tcatgagtgg atcgtccgac ggccagggac agcgttttac gttcttccgc atggtaagtg
     2161 ccctaatgtt tcagatcagc ccttgagggc gctgctgcct ttcccaatga tgttgatcag
     2221 cctcctgcgg ctgatcaatt tcgtgacaat aaaaatcatg aggcgatata tcggtccgct
     2281 gacccttcct tcccgacctt gcctctaaaa acccgatagc tatagactgt gtagccgaga
     2341 atgaccggca aaataaagac tgctcccgac aaagtgaaca acagtgtttc ccgctgcgcc
     2401 gccgcttcct gaaaggtgat ttctggtgga atggcatacg gataaataga agcaaagagc
     2461 cccagatatc cggcgaaaaa gaaaactgaa ctgaaaataa agggcgcgat ctcccgtcgg
     2521 ctacgcaaac tgcttatgat cccatagaat gaggccatac ccaatacggg gaagatccag
     2581 atgaaataaa accggggtgg tgtaagccaa tgggtcagaa ccagagggta ttgaaacggc
     2641 gtccaggcaa tcatcagaac ctggaagatc agcacaacaa gggcggcacg caaggctttc
     2701 cggtaagcgc cgttctgtac ggggcccgct gttttgatga taagataggt cgaagccaag
     2761 agtacataac cggcaatgag tccggcggcc agcgctattg aaaagggact gagccagtcg
     2821 aaagcgcctc ccgaaaaatt ttctcccgac acttttgttc ctgacaggat cccgcccagg
     2881 atcagaccct gggaaaccac cgcaaccaga cttcctgcat ggaatg
//
LOCUS       DASSLD010000479         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1352028, whole genome shotgun sequence.
ACCESSION   DASSLD010000479 DASSLD010000000
VERSION     DASSLD010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352028"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..693
                     /locus_tag="VFK23_08610"
     CDS             238..693
                     /locus_tag="VFK23_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET7319182.1"
                     /translation="MKKATIIVVAMALWIGISSASDTTKRKDVPKDLILGSLSNVYEP
                     VRFNHSGHISTAGGCADCHHQHGLVQVQSCSECHQIDPATFKKNMVAGKLKPCGECHA
                     KTDKPGRVGLKTAYHKACFKCHKADVGSGVKNLTGCTEMCHALKAKGKR"
     gene            1201..1668
                     /locus_tag="VFK23_08615"
     CDS             1201..1668
                     /locus_tag="VFK23_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319183.1"
                     /translation="MEGRRSGRVLWIGFAAFALLACGAATASDVEWQIPLIRDTVGSG
                     LTIEVSREGTRQTATPFLTLFDPTVQWIENRIGEKTAPWAAKEIPSATPREDYGRLET
                     ILSSLPPVPGEITLRSVKGFLKGLRSSLKDKPEPAVVVAGPELRIGRTDTSPR"
     gene            complement(1754..>2212)
                     /locus_tag="VFK23_08620"
     CDS             complement(1754..>2212)
                     /locus_tag="VFK23_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HET7319184.1"
                     /translation="DCSLAGRVKEAGGTTWIGLTHSARSIRAYEGLDEIWNMVARTAF
                     TQLGYSAVMLALCTLTMALTFLGPVAAVAAGPLSARLLSAGALAGMALLYRPTLAFYG
                     RSWAWSLSLPLIALLFLAMTWTSAVRFWKGDRSRWKDRVYDETMTLRRGS"
BASE COUNT          497 a          657 c          654 g          404 t
ORIGIN      
        1 gtcatcggcc agcctcttta ttttcttctg tatcgtttat gtatagttgg cttccggtga
       61 gggcttcagg aatctcccta cgctcgccgt tcttcgtcaa atctattcgg tacgttcctc
      121 ccccgcagtg agctcgaaat gcgttcgcag taaggtcata atttcttatt gcatatttca
      181 atacaatggt agtgagcatc gctcgaggaa acccctgacc gaaggaggcc ggaagacgtg
      241 aagaaagcga caatcatcgt ggttgccatg gcactctgga tcggaatctc ctccgcaagc
      301 gatacaacca aaaggaagga cgttccgaag gatctcattc tgggcagcct gtccaacgtc
      361 tacgaaccgg tgcggttcaa ccattcgggg cacatttcca cggcgggcgg atgcgccgac
      421 tgccaccatc agcacggttt ggtgcaggtt caatcgtgtt cggaatgcca ccagatcgac
      481 ccggccacct tcaagaagaa catggtcgcc ggcaagctca agccctgcgg ggagtgtcac
      541 gccaaaaccg acaaaccagg gagagtcgga ctcaagaccg cctaccataa ggcatgtttc
      601 aaatgccaca aggcagacgt gggcagcggc gtcaagaacc tgaccggctg caccgagatg
      661 tgccacgcct tgaaagcgaa aggaaagcga taagagaaac gggagctcaa cccggcttcg
      721 tgtgatcggt acgaatgaac gggagtagtt gtgcccggag gttgcggggg aatcccagga
      781 ggggtgaatc cccccgtagc cttcgggcgt gaacccgctg gaacgacgga gggaggggac
      841 aaccttcctt gccgcgccag cgcggggtga agcaaattgc gtaaaaatca cattcctgaa
      901 atcacgaaac cccaaaatgt cgaaaaacta gccgtttccc gctgaaaaac cgtaccgctt
      961 ccgttataaa gcaagtcccg tacccgcgct aattcaagac atggcgaatt tcccccattt
     1021 ttcaaaaaaa cggaaaagta aacgggtgac gccggaattg caatcctgca attcgcgcca
     1081 ccggagcgat cgcttcatcc cattgatttt acagccgttt acgaaattcc cccatggccc
     1141 cctctttgct ttcctcccag cggggaaacg gagcgaacct gggagaacgg ggggagggaa
     1201 atggagggtc gaagatccgg acgggttctg tggatcggat ttgccgcctt cgcgctcctg
     1261 gcctgcggcg ccgcaacggc ctcggatgtc gaatggcaga tcccgttgat ccgggacacc
     1321 gtgggcagcg gattgacgat cgaggtctcc cgggaaggga cccgtcagac cgcgacccct
     1381 ttccttacgc tgttcgaccc gaccgtccag tggatcgaga accggatcgg ggaaaagacc
     1441 gctccctggg cggcgaagga gatcccttcc gcgacgccac gggaagatta cggacggctt
     1501 gaaaccatcc tttcgtcgct cccgccggtg cccggggaga tcacgcttcg cagcgtgaag
     1561 ggcttcctca aagggctgcg ctcctccctg aaggacaagc cggagccggc cgttgtcgtc
     1621 gcgggtccgg aactgcggat cgggaggacc gacacctcgc cgcgctagcc tgcattcctc
     1681 atcgtatgga ttcccacgct caaaaagact ctaatcccgc tgatcgacgt ggttccctct
     1741 acggaggacc cattcaggaa ccgcgcctga gggtcatcgt ttcgtcgtag acccgatcct
     1801 tccagcggct gcgatcccct ttccagaacc ggaccgccga ggtccatgtc atggcgagaa
     1861 acaggagggc gataagcggc agggagaggg accaggccca tgatcttcca tagaatgcga
     1921 gcgtcggccg gtacaggagc gccatcccgg ccagggcgcc cgcggacagc agacgggcgg
     1981 agagaggccc tgcggccaca gcggcgacag ggcccaagaa cgtcagggcc atagtcaggg
     2041 tgcacagcgc cagcatcacc gccgaatagc caagttgggt gaacgcggtc cgggcgacca
     2101 tgttccagat ctcatcgagc ccttcgtagg cgcggatgct gcgagcggaa tgggtcaggc
     2161 cgatccatgt ggtcccaccc gcctccttga cgcgccctgc gagggagcag tc
//
LOCUS       DASSLD010000480         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_752277,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000480 DASSLD010000000
VERSION     DASSLD010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752277"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VFK23_08625"
     CDS             complement(<1..276)
                     /locus_tag="VFK23_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319185.1"
                     /translation="MKRLLTVFILLFSLFACVRAPAPVTRPEDALAPGRMHAALHDDL
                     EFRGIAASVRQSLEYYKKLPDAVFQFGPDRVTGLDMLVTLQDFLSIVE"
     gene            455..1486
                     /gene="hemE"
                     /locus_tag="VFK23_08630"
     CDS             455..1486
                     /gene="hemE"
                     /locus_tag="VFK23_08630"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HET7319186.1"
                     /translation="MSRADRFLKACRRESVDCTPVWMMRQAGRYLPEYREIRAKHSFL
                     EMCKTPELAAEVTIQPVRRYEIDAAIIFADILLPLEPMGVGLEFAKGEGPVIHNPVRT
                     MADVKRLKPIDAARQIEYLMKAIRIALRELDGKVPLIGFTGAPFTLASYIIEGGGSRN
                     YEHAKAMMFSEPKTWHRLMEHLTGVIINYLNAQIDTGVQAVQVFDSWVGALGPSDYRE
                     FVLPHQKNVIAGIKKSVPLIHFAHGAAHLLEMVSEAGGDVIGLDWRCDLDQAWKRVGY
                     DKAVQGNLDPVTLFGSKEFIRKRTKEILDRAAGRNGHIMNLGHGILQQTPLDHAEEFI
                     NSTHELSKR"
     gene            1561..2481
                     /gene="hemH"
                     /locus_tag="VFK23_08635"
     CDS             1561..2481
                     /gene="hemH"
                     /locus_tag="VFK23_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET7319187.1"
                     /translation="MNSELFFDMSHTAIILIALGGPRNLDEVGPFMAAFMGRPAPPPV
                     VSVITERYKLIGGRSPLPELVKAQAAALERELGNGRRVYEGFRYSPPSVAESFERALR
                     NGADRVIGLSMSPFATEVTTGAYRSAFEPLGPGTVGKAFIASWHDNPLFIDAWQAHVA
                     RGLERFGTAGQKDIVVIFTSHSIPVRYITAGDPYQRQVEETVKLVVDGLGLKNWRVAW
                     QSKGARATEPWLGPEVEPVLDQIAQEGHRAVLEVPVGFTCDHMETLYDIDIVHRAHAK
                     KLGLGFERAESLNTSPLFIKALADVVKNNL"
     gene            2503..2799
                     /locus_tag="VFK23_08640"
     CDS             2503..2799
                     /locus_tag="VFK23_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HET7319188.1"
                     /translation="MDSDQKDFQHKDLTDRIIKAFYVVYNNLGYGFLEKVYVSALLIE
                     LKKMGISAQTQHAINVYDDGKVVGEYFADIIVDGLVIVEAKAAKTLATDHEAQL"
     gene            2952..>3338
                     /locus_tag="VFK23_08645"
     CDS             2952..>3338
                     /locus_tag="VFK23_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7319189.1"
                     /translation="MPKIIIIGGGIAGLAAAVHLKSGSKAYDKTVDLLLLEKDKRIGG
                     KILTEKHDKFLIEAGPDSFLPEKVWSVNLARHLGLEPEMLPSNDQFKGTFIYSRNRLH
                     SLPEGVMLMVPTSFWPMAKSHLISWPG"
BASE COUNT          760 a          968 c          969 g          641 t
ORIGIN      
        1 ctccacaata ctcagaaagt cctggagcgt gacgagcatg tcgaggcccg ttacccggtc
       61 gggcccgaac tggaacacag catcgggcag cttcttgtaa tactccaggc tctggcggac
      121 ggaagcggcg atgccccgga actccaggtc atcgtgcaaa gccgcatgca tccgcccggg
      181 cgcaagcgcg tcctcgggcc tggtcacggg agcgggcgcg cggacgcagg cgaagaggga
      241 aaagagaagg atgaagactg tcagcaaccg tttcatatcc ggtgtgaaaa taccataatc
      301 tcaggggcat tgcaaccatt gacgggaaag gcggattgcc cgcgtaaagg cgtgaatcaa
      361 gaaaatttgc ttgagttttc agccgcaaaa tactattatg ggcctttgca ctaccggacc
      421 atcaactctt acggcgacag ggagagcata aagaatgtcc agggcagaca ggtttttgaa
      481 ggcatgcagg cgcgagagtg tcgactgcac cccggtttgg atgatgcgcc aggcgggcag
      541 atacctgccc gagtaccgcg agatacgggc caagcactcg ttcctcgaga tgtgcaagac
      601 gcccgagctc gccgccgagg tgacgatcca gcctgtccgg cgctacgaga tcgacgccgc
      661 catcatcttc gccgacatcc tgctgccgct ggagcccatg ggggtgggac tcgaattcgc
      721 caagggcgag ggtccggtga tccacaatcc cgtccgcacc atggcggacg taaaaaggct
      781 caagcccatc gacgccgcac ggcagatcga gtacctcatg aaggccatta ggatcgccct
      841 ccgggaactt gacggcaaag tgccgctcat cggcttcacg ggcgctccgt tcacgctcgc
      901 ctcctacatc atcgagggtg gcgggtcgcg gaactacgag cacgccaagg ccatgatgtt
      961 ctccgagccg aaaacctggc acaggctcat ggagcacctg acgggcgtca tcatcaacta
     1021 cctgaacgcc cagatcgata cgggcgtgca ggccgtccag gtcttcgact cctgggtagg
     1081 tgccctaggt ccgtccgact accgcgagtt cgtgctgccg caccagaaga acgtgatcgc
     1141 cggaatcaag aaatcggtgc cgctcatcca tttcgctcac ggggcggccc acctcttgga
     1201 gatggtctcc gaggcgggcg gcgatgtgat cggcctcgac tggcgctgcg acctcgacca
     1261 ggcctggaag cgcgtcggat acgacaaggc cgtgcagggg aacctcgacc cggtgacgct
     1321 gttcggctcg aaggagttca tccggaaaag gacaaaggag atcctcgacc gggccgccgg
     1381 caggaatggc cacatcatga acctgggcca cggcatcctt cagcagacgc cgctcgacca
     1441 cgcagaagag ttcatcaact caacgcatga attgagcaag agatagggtc acgagttcaa
     1501 agttcacagt tcagagttca gagccgaaat ctttttagcc tttactttga actgaggact
     1561 ttgaactctg aacttttttt cgacatgtcc cacaccgcta tcatactcat cgccctgggc
     1621 gggcccagga acctcgatga agtcggtccc ttcatggctg ccttcatggg ccgtccggcc
     1681 cctcctcccg ttgtttccgt gatcaccgaa cgctacaagc tgatcggcgg gaggtcgccg
     1741 ctccccgaac tggtaaaagc ccaggctgcc gccctcgaac gcgaactggg caatggtcgg
     1801 cgggtctatg agggcttccg gtattctcca ccctcggtcg cggagtcctt cgagcgggcc
     1861 ttaaggaacg gcgcggaccg cgtcatcggt ctttcgatgt cacccttcgc caccgaggtc
     1921 acaaccggcg cgtaccggag cgccttcgag ccgctgggcc cgggaaccgt cgggaaggcc
     1981 ttcattgcaa gctggcatga caaccctctt ttcatcgatg catggcaggc gcatgtggcc
     2041 cggggcctgg agcggttcgg gacagcagga caaaaggaca tcgtcgtgat attcacgagc
     2101 cacagcatcc cggtgcggta cataaccgca ggagacccgt accagcgcca ggtcgaagag
     2161 acggtgaagc tcgtcgtgga cgggctcggc ctcaaaaact ggcgcgtggc ctggcagagc
     2221 aagggggcgc gggccacgga gccctggctc ggaccggagg tcgaacccgt gctcgaccag
     2281 attgctcagg aggggcaccg ggccgttctc gaggtccccg tcgggttcac ctgcgaccac
     2341 atggagacgc tctacgacat cgatatcgtg caccgggcgc acgcgaaaaa gctggggctc
     2401 ggcttcgagc gcgccgaatc cctgaacacc tcgccgcttt tcatcaaggc attggcggat
     2461 gttgtgaaaa ataatcttta attgggacgc ggataagcgc agatggactc tgatcaaaaa
     2521 gacttccagc acaaagattt gactgatcga ataattaaag cattttacgt ggtctacaac
     2581 aatctcggct atggatttct cgaaaaagtc tatgtaagtg cgctgttgat tgagttaaaa
     2641 aaaatgggca tctctgctca aacacaacat gccatcaatg tttatgacga cggtaaagtt
     2701 gtcggtgaat attttgctga tataattgtg gatggactcg taatagtcga agccaaggct
     2761 gctaaaaccc tcgcaactga tcatgaggca cagctgtaaa attacctcaa ggcaacgact
     2821 tttgaggtcg ggttgctttt gaacttcggt ccaaaggccg aattcaagcg taaggttttt
     2881 gataactcaa gaaagtggtt tcaaatctgc gttcatctgt gcaaatctgc gtcccaaaag
     2941 gaatttgact tatgccgaaa atcatcatca taggcggcgg gatcgccgga ctggccgccg
     3001 ctgtccatct gaagagcggg tccaaggcct acgacaagac cgttgacctt ctcctcctcg
     3061 aaaaggacaa acgcatcggc ggcaagattc tgaccgaaaa gcacgacaag ttcctcattg
     3121 aagccgggcc ggacagtttc ctgccggaaa aggtctggag tgtgaacctg gccaggcacc
     3181 tggggctcga gccggagatg ctgccgtcga acgaccagtt caagggcacg ttcatctatt
     3241 cccggaacag gctccactcc ctgcccgaag gcgtcatgct catggtcccc acgtcgttct
     3301 ggcccatggc caagtcgcac ctcatctcct ggcccggc
//
LOCUS       DASSLD010000481         3800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1601630, whole genome shotgun sequence.
ACCESSION   DASSLD010000481 DASSLD010000000
VERSION     DASSLD010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601630"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFK23_08650"
     CDS             complement(<1..324)
                     /locus_tag="VFK23_08650"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HET7319190.1"
                     /translation="MGMKSFEQHRLGLRDGREVYFEGERVADVTTHPVLGAGMAFAAI
                     DYRLAEEKEHRGLAVCTDPKTGEEYSRFYRIPETAEDLLKRRELIAAGSRVNGGPPLI
                     KEIGTD"
     gene            470..703
                     /locus_tag="VFK23_08655"
     CDS             470..703
                     /locus_tag="VFK23_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319191.1"
                     /translation="MAIQKKIQKKIGSLEGQLTRRVRSLEKDVTKLVKRVEKKENEVK
                     KLKDKLTSRLVKNFKKKVKKAKKAVRKSLPAIG"
     gene            769..1512
                     /locus_tag="VFK23_08660"
     CDS             769..1512
                     /locus_tag="VFK23_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7319192.1"
                     /translation="MSNVLSVNRLRRLYGDTVAVDNVSFEVGPGEIVGLLGPNGAGKT
                     STINMILGVLEATSGTIRIEGADISTSRSRALERTNFDAVYAPLPGNLTVYQNLRVFG
                     LMYGVKEIPGRIDALLDRFDLKKHRHVKCGVLSSGEQTRVCLAKAMLNHPRLLLLDEP
                     TASLDPSAARDIRTQVREYVSQDKVAALWTTHNMYEVTDVCDRVLFLSRGRILLDGDP
                     RSLPGEHGKDTLEELFITVAREPLTLKRI"
     gene            1518..2321
                     /locus_tag="VFK23_08665"
     CDS             1518..2321
                     /locus_tag="VFK23_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7319193.1"
                     /translation="MSFHRTAAIVLRQYYLLRGSPVRVLPIFAWVAIDIVLWGFITRY
                     LNTVASPGFDFVPVLLGAVLLWDFFTRVMHGVTMAFLEDVWSRNFLNLFASPLSIREY
                     VGGLVLSSIATSLLGLLTMVVLATTAFGLSFFVYGLMLVPFLLVLFLFGIALGIFASA
                     LVLRFGPASEWIVWPIPAFLSPFAAIFYPVSTLPQWMQPVSYLLPPSCVFEGMRTIVS
                     GGAFGGTGLFLSACLAALYILLASWFFTRIHRHAVRTGLLARYTAESVS"
     gene            2408..2983
                     /locus_tag="VFK23_08670"
     CDS             2408..2983
                     /locus_tag="VFK23_08670"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET7319194.1"
                     /translation="MKKSLGAKTLVFPTPVWMVGTYDHGGRPNVMSAAWGGICCSSPP
                     CLAVSLRKATYTYGNIVKRKAFTISVPSEAYAREADYFGMVSGKNTDKFGTAGLTPIR
                     GSLVDAPYVGEFPLVIECRLLHTIEIGLHTQFVGEILDVKAEDSVCDENGYVDIERVK
                     PMLFDPEINTYHGVGRLLGRAFSIGKEIRGR"
     gene            2990..3526
                     /locus_tag="VFK23_08675"
     CDS             2990..3526
                     /locus_tag="VFK23_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET7319195.1"
                     /translation="MKFIAVTLLSAAIASFPAVQAIHAEDRKMEKLHLTSQAFTHNGM
                     IPPEYTCDGTDRNPPLTIRSVPAKSRSLALIVDDPDAPRGTWVHWVVWNIGPDTTEIP
                     ANSVPRGALQGTNDFGKQSYGGPCPPSGTHRYFFKLYALDIPLALKTGATKIQLEDAM
                     KGHILEKVELLGLYRRRS"
     gene            complement(3521..>3800)
                     /locus_tag="VFK23_08680"
     CDS             complement(3521..>3800)
                     /locus_tag="VFK23_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HET7319196.1"
                     /translation="DLMEPFKRRDIYHWRMNGSYSLKSVLPVLVPEMTYEGLEISDGA
                     MASEVYFTMETIADPAELSRLRRALLEYCKQDTLGLVRLLERMRAMGS"
BASE COUNT          754 a         1168 c         1095 g          783 t
ORIGIN      
        1 gtccgtcccg atctccttga tcagcggcgg tcccccgttg acacgcgaac cggccgcgat
       61 cagttcgcgg cgcttcagaa gatcctccgc ggtttcgggg atcctgtaga accggctgta
      121 ctcctcgccg gtcttcggat ccgtgcatac cgccagtccc cggtgctcct tctcctccgc
      181 cagacggtag tcgatcgccg cgaaggccat cccggcgccc agtaccggat gcgtcgtgac
      241 gtccgcgacc cgttcccctt cgaaatacac ctccctgccg tcgcgaaggc cgagtcggtg
      301 ctgctcgaac gatttcattc ccatcgcggt acctcctatt ccgccggact tcccggccgg
      361 ccttttccct ttagataagg cgcagagccg gggaggttcc ggacgcgctt cattcccgag
      421 cgtagggatg gtagagtgag actatctcga actacgaaag gagacggaca tggccattca
      481 gaaaaagatc cagaagaaga tcggaagcct cgagggacag ctcacacggc gcgtccgctc
      541 gcttgaaaaa gacgtgacga aattggtgaa gcgggtcgag aagaaggaga acgaagtcaa
      601 gaaactgaag gacaagttga cgtcccgcct ggtcaagaat ttcaagaaaa aagtgaagaa
      661 ggccaagaaa gcggtccgga aaagcctgcc cgcgatcggc tgacgcgatc ggttgcgggg
      721 ataacaatca cgggtgacct tgggcttcgg tcggctgagg gtgccatgat gtcaaacgtg
      781 ctctccgtca accggttgcg aaggctctac ggcgacacgg tcgcagtcga caacgtctcg
      841 ttcgaggtgg gacccggaga aatcgtcggt ctactggggc cgaacggagc cggaaagact
      901 tcgaccatca acatgatcct gggagttctc gaggcgacct ccgggacgat acgcattgaa
      961 ggcgccgaca tctcgaccag ccgttcccgg gcgctggaac gcaccaactt cgacgccgtg
     1021 tacgctccgc ttcccggcaa cctgaccgtg taccagaatc tgcgtgtttt cggacttatg
     1081 tatggcgtga aggagatccc gggacgcatc gatgcgctgc tcgaccggtt cgatctcaag
     1141 aaacaccgac acgtcaagtg cggcgtgctc tcgtcaggcg agcaaacgcg cgtatgtctt
     1201 gccaaggcaa tgctcaatca tccgcgcctc ctgctcctgg acgaaccgac ggcgtcgctc
     1261 gatccctcgg cggcgcgcga catccgcacg caggttcgcg agtacgtctc tcaggacaag
     1321 gtggccgcgc tttggaccac gcacaacatg tacgaagtca cggacgtctg cgatcgcgtg
     1381 ctcttcctgt cgcgtggaag gattcttctc gatggagacc cgagatccct gccgggtgag
     1441 catggcaagg ataccctcga ggaactcttc atcaccgtgg cgcgcgagcc gctcacgctc
     1501 aagcggatct gagcatgatg tccttccatc gcaccgcggc gatcgtcctt cggcagtatt
     1561 acctcctgcg tggaagtccg gtgcgcgtgt tgccgatctt cgcgtgggtc gccatcgaca
     1621 tcgtcctgtg gggattcatc accaggtatc tcaacacggt cgcctccccc ggcttcgatt
     1681 tcgttcctgt actcctgggc gccgttcttc tttgggactt cttcacgcgg gtcatgcacg
     1741 gcgtgacgat ggcgttcctc gaggatgtct ggtcgcggaa cttcctcaac ctcttcgcgt
     1801 cgccgctttc tattcgggag tacgtcggag gtctcgtgct ttcaagcatc gcgacgagcc
     1861 tgttgggtct cctgacgatg gtcgtccttg cgacgacggc tttcgggctc tccttcttcg
     1921 tttacggcct catgctcgta ccgttccttc tcgtgctctt cctgttcggc atagcgctcg
     1981 gcatcttcgc cagcgcactg gttctccggt tcgggcccgc gtccgagtgg atcgtctggc
     2041 cgattcctgc cttcctatcc cccttcgccg cgatcttcta ccctgtatca actctcccgc
     2101 aatggatgca gccggtctcc tatctgctcc cgccgtcatg cgtcttcgag ggaatgcgaa
     2161 cgatcgtctc aggcggagcc tttgggggaa ccgggctttt tctgagcgca tgtctcgcag
     2221 cgctgtacat cctgctcgca tcgtggtttt tcacccgtat ccaccgccat gcggtgcgca
     2281 cgggactgct cgcccgctac accgcggaaa gtgtaagcta gatcgacggc ccggctcaaa
     2341 caacacggat ttcccggcgg acagagggag tggtaaatac caggacccgc aagagggggg
     2401 aaattacatg aagaaatccc ttggcgcgaa aacgcttgtc ttccccactc ccgtttggat
     2461 ggtggggaca tatgaccatg ggggaagacc aaacgtgatg tctgcggcat ggggagggat
     2521 ctgctgttcc agccctccat gcctggccgt ctcgctccgg aaggcaacct atacctacgg
     2581 gaacatcgtg aagaggaagg cattcacgat cagcgtcccc tccgaggcct atgcccggga
     2641 ggcggattac ttcggcatgg tatccggcaa gaacactgac aagttcggca ccgccggtct
     2701 taccccgatc cggggctccc ttgtggacgc gccgtacgtc ggggagtttc cccttgtcat
     2761 cgagtgtcga cttctacata cgatcgagat cggcctgcac acccagttcg tcggagaaat
     2821 ccttgatgtc aaggcggaag attccgtctg cgacgagaat ggatatgtag atatcgagag
     2881 agtgaagccc atgctcttcg atccagagat caacacctac cacggggtcg gccggcttct
     2941 gggccgcgct ttctccatcg gaaaagagat tcgagggagg taggcttcaa tgaaatttat
     3001 cgctgtcacg ctgctgtcag ccgccatcgc atcattcccg gcggttcagg ctattcatgc
     3061 ggaggatagg aaaatggaga agctccatct cacaagtcag gcttttaccc acaatggaat
     3121 gatcccgccg gagtacacgt gcgacggaac cgacagaaat ccaccgctca cgatccggag
     3181 cgtcccggca aaatccaggt ctctcgccct gatcgtcgat gacccggatg ccccgagggg
     3241 gacatgggtc cattgggtcg tttggaatat cgggcccgac accacggaaa tcccggcaaa
     3301 ctccgtcccg agaggagcgc tccagggaac gaacgatttc gggaaacaga gctatggcgg
     3361 accttgcccg ccttccggaa cgcaccggta tttcttcaag ctgtacgccc tggatatccc
     3421 tctggcgctg aaaaccggcg ccacgaagat acagctcgaa gatgcgatga agggacacat
     3481 cctcgagaag gtggaactgc tcggtcttta ccgaagaaga tcatgaaccc atggcccgca
     3541 tcctctccag cagacggacg aggcccaagg tgtcctgttt gcaatattcc agcagcgccc
     3601 tgcgcagcct ggataactcc gcgggatccg cgatggtctc catggtgaaa tacacctcgg
     3661 acgccatcgc cccgtcgctg atctccaggc cttcgtacgt catctccggc acgaggaccg
     3721 gcaggacgga tttcagggag tacgagccgt tcatccgcca gtggtagatg tcccgccgct
     3781 tgaacggctc catcaggtcg
//
LOCUS       DASSLD010000482         2701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_573242,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000482 DASSLD010000000
VERSION     DASSLD010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573242"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..340)
                     /locus_tag="VFK23_08685"
     CDS             complement(8..340)
                     /locus_tag="VFK23_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319197.1"
                     /translation="MELDVRVGNKSMAIPIENPFRLDIDATAGRIESFLASESARAAG
                     LDFRGLLPKMIKGIAGCEGGCPADAKRFVSRGFTNFKLEYIEGGILHAQATTEDGKPV
                     SLKMFPDF"
     gene            complement(471..1592)
                     /locus_tag="VFK23_08690"
     CDS             complement(471..1592)
                     /locus_tag="VFK23_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7319198.1"
                     /translation="MPKSFESYRSEFPVTEKYIYLDHSGVAPTPLRVRRAIERFLSES
                     SEGGAFHYPAWTLRLAEIRRTCARLVNADPSEIAFVKSTSHGLSIVAEGLDWRPGDNL
                     LVYEKEFPSNIYPWLNLERKGVAVKFIPSRGGRIEIDDIENMIDPKTKLLAISSVQFV
                     NGFRIDLKRAGELCRRKNILLCVDAIQSLGAIPMDVKEFQIDFLSADAHKWLLGPEGI
                     GIFYCRLDLAERLHPPLIGWKCVQNELEFERPRFCLKADALRFEEGSMNLLGLFGLGA
                     AVDLLLEVGIENVKNRVLGLGDLVIREAKRRGFSVQTPGEREERGGNVTFSGAFDPVE
                     VRDRLRIQGIMVNVRGGGLRVSPHFYNTESELERFFQGL"
     gene            complement(1561..1857)
                     /locus_tag="VFK23_08695"
     CDS             complement(1561..1857)
                     /locus_tag="VFK23_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET7319199.1"
                     /translation="MSPRKAKSRRWYVYILKCRDGTLYTGITNDLSRREAEHNSGTAS
                     RYTRSRRPVQIIYKEPCTNRSAALKKEYAVKSLTRKEKEDYIERHAEKLRVLQK"
     gene            2087..>2701
                     /locus_tag="VFK23_08700"
     CDS             2087..>2701
                     /locus_tag="VFK23_08700"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319200.1"
                     /translation="MLCYIVVGMKSLHDMVLPVINDAGAGNKIVEVSELWGASKALFL
                     FGLRKESNRPVIIVTATEDSAGALIEDLAFFVQKMPHDAQGEGAIEIRMFPSWGVLPF
                     EADSPDSRTVGERMRFLYCLISDSPGIYVVPAQALMQKLPPWELFADSVKTITPASRI
                     EPDDLAASLVATGYEPASLVTRVGEFSRRGGIIDFFSPLHDNPVR"
BASE COUNT          586 a          794 c          703 g          618 t
ORIGIN      
        1 cagcctgcta aaagtccgga aacattttca gagaaaccgg cttcccgtct tcggtcgtag
       61 cctgggcgtg aaggatgccg ccttcgatat actcgagctt gaaattcgtg aaaccgcgcg
      121 atacgaatct ctttgcatca gcaggacatc cgccctcgca tccggcaata ccctttatca
      181 ttttcggcag caaaccgcga aagtcaaggc ctgccgccct tgcggactct gacgcaagaa
      241 acgattcgat cctgcctgcc gtcgcatcga tgtccaggcg aaaggggttt tcaatgggaa
      301 tcgccatgga tttgtttcct acgcgtacgt cgagctccat gaaaatacct ccgggccagg
      361 gataaactga attcggtaat aaaatgtaac ggcacagata ttacccaata ccacccgatt
      421 ttgcaagagg caattaccgc aggggaaggg gcttcgccgg aagcctgcgc tcacagtccc
      481 tggaaaaatc tctcaagctc ggattccgta ttgtaaaaat gaggagacac gcggagacct
      541 ccgccgcgga cattcaccat gataccctgg atgcgcagcc ggtctcgaac ctcgacagga
      601 tcgaatgccc cggaaaaggt cacgttgccg cctcgctctt ctctttcccc gggcgtctga
      661 accgaaaaac cccgtctttt ggcttcgcga atgacgaggt ctcccaaacc gagtacgcgg
      721 tttttaacgt tctcgatacc cacttcgagc agcagatcga cggcggcgcc gagcccgaac
      781 aagccgagaa ggttcatcga accctcttcg aacctcaggg catcggcctt gaggcagaaa
      841 cggggccttt caaactccag ttcattctgc acgcatttcc agccgataag gggtggatga
      901 aggcgttcgg caaggtcgag ccggcaatag aatatgccga tgccttccgg tccgagaagc
      961 cacttgtggg catctgccga gaggaagtcg atctggaact ctttcacatc catgggaata
     1021 gcgcccaggc tttggatagc atcgacgcag agcaggatgt tcttccgcct gcacagctcg
     1081 ccggctcttt tcaggtctat cctgaagccg ttcacgaact ggaccgaact gatggcaagg
     1141 agctttgttt ttgggtcaat catgttctcg atgtcatcga tctcgatcct gccgccccgc
     1201 gatggaatga acttgaccgc tacgcccttt ctttcaagat tcagccacgg gtagatgttg
     1261 gagggaaatt ccttttcata gacgaggaga ttgtctcccg gcctccagtc gagcccctcc
     1321 gcaacaatcg atagcccatg agaggtgctc ttcacgaaag cgatctccga cggatcggca
     1381 ttcacaagcc gggcgcaggt ccgcctgatt tcggccaacc gcagcgtcca ggccgggtaa
     1441 tgaaatgccc cgccctcgga ggactcggaa agaaacctct ctatcgcgcg cctgaccctc
     1501 agcggtgtcg gcgcgactcc ggaatgatcg aggtagatgt atttctccgt caccggaaat
     1561 tcacttctgt aggactcgaa gcttttcggc atgtctttca atgtagtcct ctttttcctt
     1621 gcgggtcagc gattttacgg catattcctt tttcaatgca gcggaacgat tcgtgcaagg
     1681 ctccttatag atgatttgca ccggtcgcct gctgcgagtg taccgagagg ccgtgccgct
     1741 gttgtgctct gcctcgcgac gggaaagatc gttcgtaatg cctgtataga gcgtgccgtc
     1801 gcggcatttc aggatataga cataccagcg gcgggatttc gctttccggg gactcatgag
     1861 tatttcggag acatttcagc cccgcctccg atcatacagg tcttcgacac cggggtccac
     1921 gacgagaaca taccgtttat tccgttcagc atcttccact ccggccatcc cccgtctttc
     1981 ccacaaatct tcggctccgg atatatttat tgccagacaa ctatagcaca aaaaacctat
     2041 tctatacagc cctgcccgcg gcccctgtcg tattgaatcg ggcgggatgc tgtgctatat
     2101 tgttgtggga atgaagagtc ttcacgacat ggtactgccc gtcatcaatg atgccggggc
     2161 aggaaataaa atcgtagaag tgagcgaact ctggggggcc tccaaggccc tctttttatt
     2221 cggcctccga aaagaatcga accggcccgt catcatcgtt acggcgaccg aggattctgc
     2281 aggtgcgctc attgaagatc tcgcgttctt cgtccaaaaa atgcctcatg acgctcaagg
     2341 ggaaggcgcg atcgagatcc gcatgtttcc ctcctggggg gtgctgccct tcgaggccga
     2401 ttcgcccgac agccgcaccg tgggcgagcg tatgcgtttc ctctactgtc tgatctctga
     2461 ttctcccggc atttacgtgg ttccggcaca ggctttgatg caaaagcttc cgccctggga
     2521 gctcttcgcg gactccgtaa agaccataac gccggcctcc cgaatcgagc ctgacgacct
     2581 tgccgcctcc ttggtcgcca caggctatga accggcgtcc ctggtcaccc gcgtgggaga
     2641 gttcagcaga aggggaggga tcatcgattt cttctccccc ctccatgaca accccgtccg
     2701 c
//
LOCUS       DASSLD010000483         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1415273, whole genome shotgun sequence.
ACCESSION   DASSLD010000483 DASSLD010000000
VERSION     DASSLD010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415273"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VFK23_08705"
     CDS             <1..820
                     /locus_tag="VFK23_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7319201.1"
                     /translation="SALVGLLAGVGVVTTRPDLYGGQVMSFHPWAAFAAAVALALGVA
                     YAIGIPVLKLKGHYLAMATLGFGTIIYSIVLGTQRFGAADGISGVPPFPLFPGAAVGG
                     SSSLRVLNYYIAWALVTAAMVLLTNLIHSRVGRALRSIHGAEDAAGAMGVDTARYKLY
                     TFMLSAVLAAVAGVFLTHYNGGIGPSEAGIMKSVRYVAIVAVGGMGSLWGTLIMSVVL
                     NFLSLRGYFGSFDDAVFGGILILIMLFAPDGIIGADLRALLRKILVRENITTKA"
     gene            936..1700
                     /locus_tag="VFK23_08710"
     CDS             936..1700
                     /locus_tag="VFK23_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7319202.1"
                     /translation="MVFLEVKNVSKRFGGLQAVKDVSFTVNRGCIKAVIGPNGAGKTT
                     LFNLVSGFLPPDSGTVVYRDTQINGLGPHQIAGRGLSRTFQHIRLFAHMTALENVMVG
                     CHVKSRAGFIAGMLNLPWTRREEEKIRTKSLEVMDFMGIGSLAAADAVSLSYGQQRAV
                     ELARALAGEPAMLLLDEPAAGLNMRETADLAGLITRVRDLGITVLIVEHDMSLVMNIS
                     DEVVVLSYGEKIADDTPRAVQNNPEVVRVYLGEDDA"
     gene            1693..2424
                     /locus_tag="VFK23_08715"
     CDS             1693..2424
                     /locus_tag="VFK23_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7319203.1"
                     /translation="MLKLRNLDAGYGSLSVLRRVSLHVKPGEIVTIIGANGAGKTTLL
                     KTVTGLLRAQSGEIIFNNEDITRAQTERIVSGGCSLVPEGRQVFPAMPVSENLLLGAY
                     VQLKRGNKREVERVLGHVYGLFPVLKQRERQLAGTLSGGEQQMLAIGRALMAGPRLIM
                     LDEPSMGLAPLMVKEIFNIIKRLRDEGNTVLLVEQNARSALKIANRGYVLETGRIILE
                     GDAEDLLANRDVQRAYLGRDIDENI"
BASE COUNT          440 a          758 c          776 g          472 t
ORIGIN      
        1 atcggccctg gtaggcctcc tggcgggcgt cggggtcgtc acgacgagac cggacctcta
       61 cggcggtcag gtcatgagtt tccacccctg ggccgcattc gccgctgccg tggcgctggc
      121 cctcggggtc gcgtacgcca tcggcatccc ggtcctgaaa ctcaagggcc attacctcgc
      181 catggccacg ctcggcttcg gcacaatcat ctacagcatc gtcttgggga cgcaacggtt
      241 cggcgcggcc gacgggattt cgggcgtgcc gcctttcccg cttttccccg gcgccgccgt
      301 cggcggcagc tcgtcgctcc gggtgctgaa ctactacatc gcctgggccc tcgtgacggc
      361 ggccatggtg ctactcacca acctcatcca ttcgcgggtc ggccgggcgc tccgctcgat
      421 ccacggggcg gaggacgccg cgggcgccat gggcgtggac acggcccggt acaagctcta
      481 caccttcatg ctctccgccg tgctggccgc ggtggcgggt gttttcctca ctcactacaa
      541 cgggggcatc ggcccctcgg aggccggcat catgaagtcc gtgcgctacg tggcgatcgt
      601 cgccgtgggc ggcatgggga gcctctgggg cacgctcatc atgagcgtcg tcttgaattt
      661 tctctcgctg cgcggctatt tcggctcctt cgacgacgcc gtgttcgggg ggatcctgat
      721 tctgatcatg ctcttcgccc ccgacgggat catcggcgcg gatttgagag ccttgcttcg
      781 aaagatactc gtcagggaga acataacgac gaaggcttga tttagccaca gaggatacgg
      841 agaaaagctg aggttaggaa cgcgcggtgt gtgattcgag aattttctct gtggtctctg
      901 tgcgctctgt ggcaaaaagg ttttctcttt ttactatggt ttttcttgaa gtaaaaaacg
      961 taagcaagcg cttcggcggg ctccaggccg tgaaggatgt aagctttacg gtgaaccgcg
     1021 gctgcatcaa ggcggtcatc ggaccgaacg gcgcgggaaa gacgacgctc ttcaacctcg
     1081 tctcgggctt cctcccgccc gactccggga ctgttgtcta ccgtgacacg cagatcaacg
     1141 gactgggccc ccatcagatc gcggggcggg gcctgtcgcg caccttccag cacatcaggc
     1201 tctttgcgca catgaccgcc ctcgagaacg tcatggtggg atgccacgtg aaaagccgcg
     1261 ccgggttcat cgccggcatg ctgaacctcc cctggaccag gcgtgaggag gagaagatcc
     1321 ggacaaagtc cctcgaggtc atggacttca tgggtatcgg cagccttgcc gcggccgacg
     1381 ccgtgagtct ttcctacggc cagcagcgcg cggtcgaact tgcccgcgcg ctcgcgggcg
     1441 agccggcgat gcttcttttg gatgagcccg ctgccgggct caacatgcgt gagaccgctg
     1501 acctggcggg gctcatcacc cgggtccggg acctcggcat caccgtcctg atcgtcgagc
     1561 acgatatgtc cctggtcatg aacatttccg acgaggtcgt cgtcctctcc tacggcgaaa
     1621 agatcgccga tgacacgccc cgcgccgtcc agaacaatcc tgaggttgtc cgggtctacc
     1681 tgggggagga cgatgcttaa gctgcggaat ctcgacgcgg gatatgggag cttgagcgtt
     1741 ctgcggcgcg tgtcgctgca cgtaaagccc ggcgagatcg tcacgatcat cggcgcgaac
     1801 ggagcaggaa aaaccacgct cttaaagacc gtcacgggac ttctccgggc gcagtccggc
     1861 gaaatcattt tcaacaatga ggacatcacc cgggctcaga cggagcggat cgtatcgggc
     1921 ggctgctcgc tcgttcccga gggcaggcag gtttttcctg ccatgccggt ttcggaaaac
     1981 ctccttctcg gggcctatgt ccagttgaag cgcggcaaca aacgcgaggt ggagcgcgtg
     2041 ctcggccatg tctacggtct tttcccggtc ctgaagcagc gggaacggca gcttgccggc
     2101 accctgtccg gaggagagca gcagatgctc gcgataggcc gggcgctcat ggcggggccg
     2161 cgtctcatca tgctcgacga gccgtccatg ggcctcgccc cgctcatggt gaaggagatc
     2221 ttcaacatca tcaagcggct ccgggacgag ggcaacacgg tcctgctcgt ggagcagaac
     2281 gcgcggagcg cgctcaagat cgcgaaccgc ggctacgtgc tcgaaaccgg caggatcatc
     2341 ctcgaaggcg atgccgagga cctcctggcg aaccgggacg tgcagcgggc gtatctggga
     2401 agagacatcg atgagaatat ctaaatacga aactcgaaac tctaaa
//
LOCUS       DASSLD010000484         2763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1381651, whole genome shotgun sequence.
ACCESSION   DASSLD010000484 DASSLD010000000
VERSION     DASSLD010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381651"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /locus_tag="VFK23_08720"
     CDS             complement(<1..1601)
                     /locus_tag="VFK23_08720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7319204.1"
                     /translation="MKKKILVFLALSLLLASAVGTYLLVTIERRASDLNELIILHEVE
                     ILRERLLLDIRRVEADLYSQNTRHPESIDALTNHINDMQSTIKTCLDCHHSPDVLERL
                     RDLDHQIDQYSLDVGRIMAREVKPGRLPDETERVHLIGDSLISKINTMIVLTNKKLNE
                     RTEGSLREMQRTKIIVLMLVIAGPLMIAILGFTATSHFTRPIQTLLEATGKLKAGSFD
                     FRVSGLKNEFAELAVAFDDMCGALREHMRAIEESEKRYRLLFDSAPDAIFILDAEGEN
                     AGKILQANSAASKMHGYTVEELCTMKIADLDSPEAAIEVPDRIKRLLGGEWLQAKVNH
                     RKKDGAVFPVEIRAGIFEVGDRRYILAIDRDVTERTLAEEALRRAQQVKTAGEMATGL
                     AHEIKNPLAGIKVTMEILAEEPHLSDENRDMLAKVIGEIKRIEYLMKGLLNFAKPPRP
                     QLARTDVNAVLETVASLALKDRTPSRDGSHAIHVVKDYQQDIPKVLADQMQLQQILMN
                     LVLNAADAMPKGGTVFLKTHFSRAS"
     gene            2121..>2763
                     /locus_tag="VFK23_08725"
     CDS             2121..>2763
                     /locus_tag="VFK23_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319205.1"
                     /translation="MKKSMLTSIAAGLFIVLSACATSYVAKPLPFKMPGTYPNAQEIA
                     GAVIGAHAYTNVKEAKENFGFDVINAGMLPVEIVIDNKGPHPLEVNGTQTFLEDRQNN
                     LWPVLSRNLAYDRATKYAQTKETFSEAAHSGFFGAAAGAAIGAAIGILTGTNVANAAG
                     KGAAVGAAGGAVIGGAQAYDAGDARHAIVDDLQQKSLQAKAIAPNTLAYGFIFF"
BASE COUNT          553 a          774 c          809 g          627 t
ORIGIN      
        1 ttcgaagccc tgctaaagtg cgttttcaga aacaccgtcc cccccttcgg catggcgtct
       61 gcggcgttca gtacgagatt catgagtatc tgctgaagct gcatctgatc ggccagcacc
      121 ttcgggatgt cctgttggta gtccttgacg acatggatcg catgcgaccc gtcccgggag
      181 ggtgttcggt cttttagcgc caggcttgcc acggtctcga ggacggcatt cacatcggtc
      241 ctggcgagct gcggccgcgg cggctttgcg aagttcagaa gtcccttcat caggtattcg
      301 attcgtttga tctcgccgat caccttggcg agcatgtccc ggttttcatc ggacaggtgc
      361 ggctcctcgg cgagaatctc catggtgacc ttgatgcccg ccagcggatt cttgatctcg
      421 tgcgccaggc ctgtcgccat ctctcccgcg gttttgacct gctgcgcgcg ccgcagcgcc
      481 tcttctgcca gagtcctttc ggtgacgtcg cggtcgatcg caaggatgta ccggcggtca
      541 ccgacttcaa agatgcccgc gcgtatctcg acggggaaca cggcgccgtc ttttttgcga
      601 tggttgactt tggcctggag ccactcccct cccagcaacc gcttgatccg gtcgggtact
      661 tcgatggccg cttcgggact gtcgaggtcg gcgatcttca ttgtacaaag ttcttcgacg
      721 gtatagccgt gcatttttga cgcggcagaa ttggcctgca ggatcttgcc ggcattctcg
      781 ccttccgcgt ccagtatgaa aatggcgtcg ggtgcgctgt cgaacaggag gcgatagcgc
      841 ttttcgctct cctcgatcgc ccgcatgtgc tccctgaggg cgccgcacat gtcgtcgaag
      901 gcgacggcca gttccgcgaa ctcgttcttg agtcccgaga cccggaaatc aaaacttccg
      961 gccttgagtt ttcccgtcgc ctcaagcagc gtctggatcg gtctcgtaaa atggctcgtt
     1021 gccgtaaagc ccaatattgc gatcatcagg gggccggcga tgacgagcat gagcacgatg
     1081 atcttcgtcc gctgcatctc gcgcagagag ccctccgtac gttcgttgag tttcttgttt
     1141 gtcagcacga tcatcgtgtt aattttactg atgagcgaat cgccgatgag gtggacccgt
     1201 tccgtttcgt ccggaagacg ccccggcttc acctcccggg ccatgatcct gcctacgtcg
     1261 aggctgtact gatcgatctg atggtccaga tcgcggagcc gctccagcac atccggcgaa
     1321 tggtggcaat caaggcaggt cttgatcgtg ctttgcatat cgttgatatg atttgtgagg
     1381 gcatcgatgc tctcgggatg ccgggtgttc tgagaataga ggtccgcctc gacccggcgt
     1441 atgtccagca ggagacgctc tcgcaggatt tcaacttcat ggagaatgat gagttcattc
     1501 agatcggatg cgcgccgctc gatggttacg aggagatacg tgccgacggc ggacgccagc
     1561 agaagggaaa gggccaggaa tacgaggatc ttttttttca tgcaatcaac tcagaactgt
     1621 gtcgggcccg ccgagatccc gttcagagcg caacgggttt cgcattgtat gcatgagcgc
     1681 ggccagtttt gccgcggcac accgcttatc caatcggaga gtaaatctgg agagtgttct
     1741 caagggcctc tggggatgtc cttttttgca tggtgacaac tggccacaag catcatatat
     1801 caccggttga gcgctgtcaa tatccaaaaa aacaatatct tggaatggtt cttgccggta
     1861 tcttggcagc acaggcgtta catatgtcac gtgacgcccc ggcaatggcg gcccggaacc
     1921 tgctacgaac aaggtagtac gatatggccg gtatatttct cgcggtgcgc gtgagtgata
     1981 taatgatcat ggagaatctc ggccggacca aaagcggaaa gccgctgcgt ccggaacgag
     2041 acggcgtttc cattcatgtg caggagattc cgctcttgtt aaacctgttg agcgatgaac
     2101 gccggaagag gaggtttatt atgaaaaaat ccatgttaac ttcaattgca gcgggcctgt
     2161 ttatcgttct ctcggcctgt gcgaccagct atgtggcaaa accgcttccc ttcaagatgc
     2221 ccgggacgta tccgaatgcc caggaaatcg cgggcgccgt gatcggtgcg cacgcgtaca
     2281 caaacgtgaa agaggcaaaa gagaacttcg gcttcgacgt catcaatgcc ggaatgctgc
     2341 cggtggagat cgtaatcgac aacaagggtc cccatcccct cgaggtgaac gggactcaga
     2401 cgttcctcga ggacaggcag aacaacctct ggccggtgct cagcagaaac ctggcctatg
     2461 accgagcgac aaaatacgcc cagacgaagg aaaccttcag tgaggccgcc cattcgggct
     2521 ttttcggcgc ggccgcgggc gcggccatcg gagccgcaat cggcattctc accggcacga
     2581 atgtggctaa tgcggcgggg aagggagctg ccgtgggtgc ggccggcggc gcagtcatcg
     2641 gaggagccca ggcgtatgat gcaggtgacg cgcggcatgc catagtagac gaccttcagc
     2701 agaagtccct tcaggcaaaa gcgattgcgc ccaataccct tgcctacggg ttcattttct
     2761 tcc
//
LOCUS       DASSLD010000485         4535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_566596,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000485 DASSLD010000000
VERSION     DASSLD010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566596"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VFK23_08730"
     CDS             <1..734
                     /locus_tag="VFK23_08730"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HET7319206.1"
                     /translation="ADILGTILQKTDRFIVIPQQDMDSVLGQQRMGATGAINPDTAAK
                     MGQILGLNAIVTGAVTAYSEAEEGSDYLITKSKKQIARVTVDYRIVDTTTGVQLMADS
                     GAGIYEKKVSTTLGMGGKAGYDSDLRDGALRDALTKAMVNMMKQLESQPWKGKIAKVA
                     GNKLYINAGKKTGLKVGDKLDVYRVGEDIIDPDTHMKLGTTEDRVGQAIIQQNDIGDK
                     ADMSIAITTSGMGFKQGDIVKFVSK"
     gene            772..1530
                     /locus_tag="VFK23_08735"
     CDS             772..1530
                     /locus_tag="VFK23_08735"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HET7319207.1"
                     /translation="MRKRSGFALVLLLALVVFAGEVHAVDPGGSEATVTTVRGSAKVY
                     RSGSTAGRPLHKGDKLKKDDEVKVAEKSRLEIIFADGTVMRLAERSSLRMNELVFDKE
                     TDRKNVKVGLSLGKLWANVKKLKTDDSSVEVKTSNAVAGVRGTVYRVNVDEDKSALVK
                     VYDGEVYVANPPRDASKPIDKATAPHEVAGPHAVPPPYHEVSMEEWTAIVKAMQQIRI
                     SPDGKPSQPEDFDPKADADDWVKWNQKRDKELSL"
     gene            1687..2124
                     /locus_tag="VFK23_08740"
     CDS             1687..2124
                     /locus_tag="VFK23_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET7319208.1"
                     /translation="MQRASQQLSEYLSGQGLRSTTQRDDILQVFVDAGQHLSAAELYS
                     RVRKNHPGIGYATVYRTLKLLADAGLADERRFEDGFTRYEYKSPNSHHDHLICTKCGA
                     IIEFENERIEELQMDVAKKNQFLVKSHKLELYGLCSACQKNKR"
     gene            2144..4129
                     /gene="feoB"
                     /locus_tag="VFK23_08745"
     CDS             2144..4129
                     /gene="feoB"
                     /locus_tag="VFK23_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HET7319209.1"
                     /translation="MHDHGSNEGERFRDSNLNKVLLIGNPNVGKSALFGLLTGKYVTV
                     SNYPGTTVEVTYGNAVLNKARALVIDTPGVNSLVPMSEDEKVTRDMLISDRAGVVVQV
                     ADTKNLRRGLLITLQLAEMEVPFILDLNMDDEAKSRGILVDQEKLSGLLGIEVVKTVA
                     IRRSGIDKLMKGIQHPRKSIVALRYDDAIEDGVRDISALLPPANISRRALALMVLAGD
                     ESLKGWLHTNLQDHVIEELDKIRETVQSKFHNSLGYVINSRRIKKADEILAQVMTRHE
                     AGKGSMVAFHLGRLSMHPLYGLPILFAVLYLVYQFVGVLGAGDAVGFLEKTVFGEYLN
                     PWATKLSNILVPIPLIQELLVGEYGLITMALTYSIAIVLPIVGFFFIAFGILEDSGYL
                     PRLAVMVNKIFKIMGLNGKAVLPMVLGLGCDTMATLTTRILETKKERILVTLLLALGV
                     PCSAQLGVVLGMVSGISGYATAVWIGTVVLVMLLVGYLAAKAIPGEPSDFVLELPPIR
                     VPQLSNILIKTLARVQWYLKEAVPLFILGTFILFVGHKLGALAYIQKMTDPLVVGILG
                     LPSKAAEAFVIGFLRRDYGAAGLFVLAKNGMLDPIQTVVSLVVITLFVPCIANFFMMV
                     KERGMKAALYMVAFIFPFAFAVGGALNWILRTFKVAL"
     gene            4174..>4535
                     /locus_tag="VFK23_08750"
     CDS             4174..>4535
                     /locus_tag="VFK23_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HET7319210.1"
                     /translation="MAEHKRIDAVEGKPGHPALCVLPEIEKEDELFEAMWTLREDGKL
                     NRDNLIESCTIEGCEAILQELAKNGWIEAKGGNIELLPKGEKRAKELVRRHRLSLRMF
                     YDLFALDGAEAEACKFEH"
BASE COUNT         1061 a         1216 c         1371 g          887 t
ORIGIN      
        1 cggcggacat tctgggaacg attcttcaga agaccgaccg cttcatcgtt attccgcagc
       61 aggatatgga ctctgttctg ggccagcagc ggatgggggc cacgggggcg atcaatcccg
      121 atacggcggc gaaaatgggc cagatattgg gtcttaacgc gatcgtgacc ggcgcggtca
      181 ccgcctattc cgaggccgaa gagggcagcg actacctcat tacgaagagc aagaagcaga
      241 tcgcccgggt cacggtggat taccgcatcg tcgacaccac gacgggcgtc cagctcatgg
      301 ccgattcggg cgccggcatt tacgagaaga aggtatcgac gacgctcggg atgggcggca
      361 aggcgggtta tgactccgat ctgcgcgacg gcgcgctccg ggacgccctc accaaggcta
      421 tggtaaacat gatgaagcag cttgagagcc agccgtggaa gggcaagatc gccaaggttg
      481 ccggcaataa gctctatatc aatgccggca aaaaaaccgg actgaaagtg ggggacaagc
      541 tcgatgtata tcgggtcggc gaggatatta tcgacccaga cacccacatg aagctgggaa
      601 cgaccgagga cagagtgggc caggccatta ttcagcagaa cgacatcggt gacaaggcgg
      661 atatgtccat agcgatcacc acgtcaggca tgggtttcaa acagggtgac attgtaaagt
      721 ttgtctccaa gtagcgatgg accatatcgc attccggaga ggacaagacg tatgagaaaa
      781 cgcagcggtt tcgccctggt cttgttgctg gcccttgtgg tgtttgcggg cgaagtgcat
      841 gcggtcgacc cgggcgggag cgaggccacg gtcacgaccg ttcgcgggag tgccaaagtc
      901 taccggagcg gttcgacggc tggccggccg ctgcacaagg gggacaagct caaaaaggac
      961 gacgaggtaa aggtcgcgga gaaatcacgg ctggagatta ttttcgccga cgggaccgtg
     1021 atgcggcttg ccgagaggtc gagtctcaga atgaacgaac tggtgttcga caaggagacg
     1081 gaccgtaaaa acgtaaaggt cggcctttct ttgggaaagc tctgggccaa tgtaaagaaa
     1141 ctgaagaccg atgattcgtc ggtagaggtc aagacatcga atgccgtcgc cggcgtccgg
     1201 gggacggtgt accgggtcaa tgtggacgag gacaagagcg ccctggtcaa ggtgtatgac
     1261 ggggaggtct acgtggcgaa tcctccgagg gacgcctcga aaccgatcga caaggcgacc
     1321 gcgcctcatg aggtggccgg tccgcacgca gtgccgccgc cctatcacga agtgtcgatg
     1381 gaggaatgga ccgccattgt gaaggccatg cagcagatca gaatatcccc cgacgggaaa
     1441 ccctcgcaac cagaggattt cgatcccaag gcggatgccg acgactgggt gaagtggaac
     1501 cagaaacgcg acaaggagtt gtcgctttag ttcctgcgca tctgagacat caatatttca
     1561 ggaaggccgt cccgcaagga cggccttttt attgtgaggg agggaaaaaa cgcttgacag
     1621 cgccgcccgc gcatggtata aatatgcgct agttgaaaat ggatttcaat atcatttatg
     1681 aagactatcc agagagccag tcagcaacta tcggaatatc tgtcagggca ggggctgagg
     1741 tccacgaccc agcgtgacga tattttgcag gtctttgtcg atgccgggca gcacctgagc
     1801 gccgcagaac tctacagtcg ggtgagaaaa aaccacccgg gtatcggtta tgccaccgtt
     1861 taccgcaccc tgaagctgct ggccgatgcg ggactggccg acgaacgaag attcgaggac
     1921 gggtttacac ggtacgagta caagtccccg aacagccatc acgaccacct catctgcacg
     1981 aaatgcggcg cgatcatcga attcgaaaac gagcgcattg aagagctcca gatggacgta
     2041 gcgaaaaaaa atcaattcct ggtaaagagc cacaagctgg aactctacgg cttgtgttcg
     2101 gcatgtcaaa agaacaaaag atagactcgg aggttttaga gtaatgcatg atcacggctc
     2161 caacgagggc gaacgattcc gcgactccaa cctgaataag gtcctcttga tcggaaaccc
     2221 gaacgtgggc aagagcgcgc tcttcggcct gctcacgggc aaatacgtta ccgtttccaa
     2281 ctatccgggc accaccgtgg aagtgaccta cgggaacgcc gtcctgaaca aggcccgtgc
     2341 gctcgtcatc gacacgccgg gtgtaaacag cctggtacca atgtcggagg acgaaaaagt
     2401 tacgcgggac atgctgatct cggaccgggc gggcgtcgtc gtccaggtgg cagacaccaa
     2461 gaacctgcgg cgcggtctgt tgatcacgct ccaactcgcc gagatggaag tgccgttcat
     2521 cctggacctc aacatggacg acgaggccaa gagccgcggc attctcgtcg atcaggagaa
     2581 actgtccggc ctcctgggga tcgaggtcgt caagaccgtt gccatcagac ggagcggcat
     2641 cgataagctc atgaagggca ttcagcatcc ccgaaagtcc atcgtagcgc ttcgctatga
     2701 tgatgcgatc gaagacggag tgagggacat ctcggccctt ctgcctcccg cgaacatctc
     2761 gcggcgcgcg cttgcgctca tggtcctggc cggcgacgaa agcctcaagg gctggctcca
     2821 tacgaacctc caggatcatg tcatcgagga gctcgataag atccgagaga ccgtccagtc
     2881 gaagttccac aactcgctgg gatatgtcat caacagccgg cggatcaaaa aagccgatga
     2941 aattctcgct caggtgatga cccggcatga ggcggggaaa ggttccatgg tggcgttcca
     3001 tctcggacgt ctgtcgatgc accccctcta cggtctgccg attctttttg ccgtactgta
     3061 tctcgtctat cagttcgtgg gagtactcgg ggcgggcgac gccgtgggat tcctggaaaa
     3121 gacggtgttc ggcgaatatc tgaacccctg ggcgacaaag ctttctaata tcctcgtacc
     3181 catcccgctc atccaggaac tgctcgtggg tgaatacgga ctgatcacca tggccctcac
     3241 ctattccata gcgattgtgc tgccgatcgt cgggtttttc ttcatcgctt tcggtatcct
     3301 cgaggattca gggtatctcc cgcgacttgc ggtcatggtg aacaagatat tcaaaatcat
     3361 ggggttgaac ggaaaagcgg tgctcccgat ggtactgggg ctcggctgtg acaccatggc
     3421 gaccttgacg acgcgcattc tcgaaaccaa gaaagagcgc atcctcgtga cgcttctctt
     3481 ggcgcttggc gtgccctgtt ccgcgcagct cggcgtcgtc ctgggcatgg tgtccggcat
     3541 ctccggttac gccaccgccg tctggatcgg gacggtggtc ctggtgatgc tgctcgtggg
     3601 gtatctcgcc gccaaggcca ttcccggaga accctccgat ttcgtcctcg aattgccgcc
     3661 catccgcgtg cctcagcttt cgaacatcct gatcaagacc cttgcacggg tccaatggta
     3721 cttgaaagag gctgtgccgc ttttcatcct ggggacattc atcctcttcg tggggcacaa
     3781 gctcggcgcg ctcgcctata tccagaagat gaccgatccg ctcgtcgtcg gaatactcgg
     3841 tcttccgagc aaggcggcgg aggcctttgt catcggattt ctccgcagag actacggcgc
     3901 ggccggtttg ttcgttctgg ccaaaaacgg gatgctcgac ccgatacaga ccgtggtgag
     3961 cctcgtggtc ataaccttgt tcgtgccctg catcgcaaat tttttcatga tggtgaagga
     4021 acgggggatg aaggccgcat tgtacatggt ggccttcatc tttccttttg cctttgccgt
     4081 aggcggagca ctgaactgga ttctcaggac gtttaaagtc gcattgtaat aagcacaaac
     4141 cgacccctag gtagaggtct aaaaggagaa actatggcgg aacacaaaag gatcgacgcc
     4201 gttgagggca agccgggcca tcccgcgctc tgcgtactgc cggaaataga aaaggaagat
     4261 gagctcttcg aggcaatgtg gacccttcgg gaagacggaa agctcaaccg ggacaatctc
     4321 atagagagct gcaccattga ggggtgcgag gccatactgc aggaactcgc gaagaacggc
     4381 tggatcgagg cgaaaggcgg gaacatcgag cttcttccga aaggggagaa gcgggcaaaa
     4441 gaactggtcc gcaggcaccg gctgtcgctc cggatgttct atgacctttt cgcgctggac
     4501 ggtgccgagg ccgaggcgtg caagttcgag catat
//
LOCUS       DASSLD010000486         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1503174, whole genome shotgun sequence.
ACCESSION   DASSLD010000486 DASSLD010000000
VERSION     DASSLD010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503174"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..201)
                     /locus_tag="VFK23_08755"
     CDS             complement(61..201)
                     /locus_tag="VFK23_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HET7319211.1"
                     /translation="MRIAGIQISSSPDIGRNVQRAVEMAELAAEKNAGIVCFPELFTI
                     PK"
     gene            complement(278..>2248)
                     /gene="secA"
                     /locus_tag="VFK23_08760"
     CDS             complement(278..>2248)
                     /gene="secA"
                     /locus_tag="VFK23_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET7319212.1"
                     /translation="LIISGPAEESTDKYYKINRIIPSLKKDADYTIEEKTKTAALTEE
                     GNIKVERLLGAGNLYDPANVDLVHHVHQALKAHALFHRDVDYVVKDDQVMIVDEFTGR
                     LMPGRRWSDGLHQAIEAKEGVKIENENQTLASITFQNYFRLYGKLAGMTGTAETEAAE
                     FAKIYNLDVVVIPTHKPMIRVDNPDFIYKTEQAKFNAAIGEIEGLHQKGQPVLVGTIS
                     IVKSETLASMLKKKGLKHTVLNAKYHEMEAEIVAQAGRHAAVTIATNMAGRGTDIVLG
                     GNPEGLTRQALKGKEYTKEEWDKTFEQFKAVCAKEKEKVIEAGGLHILGTERHESRRI
                     DNQLRGRSGRQGDPGSSRFYLSLEDDLLRIFGSDRISGLMGKLGMEEDVPIEHRMVSR
                     AIENAQKKVEAHNFEIRKHILEYDDVMNQQRKVIYEQRREILAGTDLKEEVLGLAEDM
                     LDGMINFYCPEDAYSEQWDITGLTDALAAKFSFRLAGIDPHSLSRDEFRQKLWEMIKQ
                     SYDDKEKEIGPELMRHLEKVFMLQAVDHQWKDHLLAMDHLKEGIGLRGYGQKDPLIEY
                     KKEGFDMFEEMKLRIVETTVENLFRIKVAAKQQGEEIERKQRRQMANLSFVGAGDAAA
                     AKPAPVHAAQKVGRNEPCPCGSGLKYKTCHGK"
BASE COUNT          357 a          682 c          659 g          550 t
ORIGIN      
        1 atcagaaaaa ttcggatgga ggaccactga gataaacaag gcgaccgaat gggtagctaa
       61 tcatttcggt attgtgaaga gctccggaaa gcagacgatc ccggcgttct tttcggcggc
      121 cagttcggcc atctccaccg cgcgctgaac gttgcgcccg atgtccggac tgctgcttat
      181 ctggatgccg gcgattcgca tagttagttc ttagctcatg gctcgtaacg cgtagcaact
      241 actatcagct acgagctatc agctacaagc tgctgcgtca cttcccgtga cacgtcttgt
      301 acttgagccc gctcccgcag gggcagggct cgttccgtcc caccttctgc gccgcgtgca
      361 ccggcgcggg ctttgctgcg gcggcatcac ccgccccgac gaagctcagg ttcgccatct
      421 ggcgccgctg cttgcgctcg atctcctccc cctgctgctt ggccgcgacc ttgatgcgga
      481 agaggttctc caccgttgtt tccacgatgc ggagcttcat ctcctcgaac atgtcgaagc
      541 cttctttttt atactcgatc agggggtcct tctgcccgta tcccctgagt ccgattcctt
      601 ccttcagatg gtccatggca agaaggtggt ccttccactg gtggtccacg gcctgaagca
      661 tgaacacctt ctcaaggtgg cgcatgagct cggggccgat ttccttttcc ttgtcgtcgt
      721 agctctgctt gatcatttcc cacagcttct gccggaactc gtcgcgggaa agggaatgcg
      781 gatcgattcc cgccaggcgg aaggaaaatt tggctgccag cgcgtcggtg aggccggtga
      841 tgtcccactg ctcggaatag gcgtcctcgg ggcagtaaaa attgatcatg ccgtcgagca
      901 tgtcctcggc caggcccagc acctcttcct tgaggtccgt cccggccagg atctcccggc
      961 gctgctcgta gatcaccttt cgctgctggt tcatcacgtc gtcgtattcg aggatgtgct
     1021 tgcggatctc gaagttgtgg gcctccacct tcttctgcgc gttctcgatg gcgcgcgaca
     1081 ccatgcggtg ctcgatgggc acgtcctctt ccatgccgag cttgcccatg aggcccgaga
     1141 tgcggtcgga gccgaagatg cgcaggagat catcttcgag cgagaggtag aaccgggacg
     1201 agccggggtc gccctgcctt cccgagcgtc cgcggagctg gttgtcgatc cggcgggact
     1261 cgtgccgctc cgtgcccagg atatggagac cgcccgcttc gatgaccttt tctttttcct
     1321 tggcgcacac ggccttgaac tgttcaaacg ttttgtccca ttcttccttg gtatattctt
     1381 ttcccttgag cgcctgacgc gtgagccctt cggggttgcc gccgagtacg atgtcggtgc
     1441 cgcggcccgc catgttcgtg gcgatcgtga ccgcagcgtg ccttccggcc tgggcgacga
     1501 tctcggcctc catctcgtgg tacttggcgt tcaggaccgt gtgcttgagg ccctttttct
     1561 tgagcatcga cgccagggtt tcggacttga cgatcgagat agtgcccacg agcacgggct
     1621 ggcccttctg atgcagtcct tcaatctcgc cgatggcggc gttgaacttc gcctgctcgg
     1681 tcttgtagat gaaatcgggg ttgtcgacgc ggatcatggg cttgtgcgtg ggaatcacga
     1741 ccacgtcgag gttgtagatc ttcgcaaact ccgcagcctc ggtctcggcg gtgccggtca
     1801 tgcctgcgag cttgccatag agccggaaat agttctggaa cgtgatcgac gccagggtct
     1861 ggttctcgtt ctcgatcttg acgccttcct tggcctcgat ggcttggtgc agcccgtcgc
     1921 tccagcgcct gcccggcatg agccttcccg tgaactcgtc cacgatcatc acctggtcgt
     1981 ccttcacgac gtagtccacg tcgcggtgga acagggcatg ggccttcagg gcctggtgca
     2041 cgtgatgcac gaggtcgacg ttcgcggggt cgtagagatt gccagcgccc aggagccgct
     2101 ccactttgat gttcccttcc tcggtcaggg ccgcggtctt ggtcttctct tcaatcgtat
     2161 aatcggcgtc cttcttgaga ctcgggatga tccggttgat cttgtaatat ttgtcggtgg
     2221 actcttccgc gggaccggaa atgataag
//
LOCUS       DASSLD010000487         4685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_237097,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000487 DASSLD010000000
VERSION     DASSLD010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237097"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(678..842)
                     /locus_tag="VFK23_08765"
     CDS             complement(678..842)
                     /locus_tag="VFK23_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319213.1"
                     /translation="MCYNCGCGKPGDDMGDPRNITDKTFEEASKASKQSPKDAKKNTL
                     ELLKIILKKE"
     gene            complement(934..1278)
                     /locus_tag="VFK23_08770"
     CDS             complement(934..1278)
                     /locus_tag="VFK23_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET7319214.1"
                     /translation="MAFGPCRSGGSDGVRTVVSVGGEAEGTPIVSNLGRISIGADASK
                     QLASDHALELGAIKAYNQAIKLAGDVDDFATREILEDILQNEDAHVDRIEALQDQIGD
                     MTLPMYLTTQVG"
     gene            complement(1227..2351)
                     /locus_tag="VFK23_08775"
     CDS             complement(1227..2351)
                     /locus_tag="VFK23_08775"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET7319215.1"
                     /translation="MRGKGTLCIKGVIILLTALVLQGTNWAAAEELTVMTRNLYLGAD
                     LIPLIKAQTEAQFESAVFDILGTIEANSFPERAAALAAEIVEKKPHLVGLQEVLNFTI
                     DGANSRPPFYDYLQELLNALKAQGANYRVVAVVKNLDLVFPFDINRIGVPNLIGALDR
                     DVILARDDVKDVVVLTGLCPESQVSDNGCNYQFAASLPNPLEPLDNIVIKRGFVAIDA
                     LVDGFPVRFVNTHLEGRDFGPLLQAAQAFELTQILAALNSELGAPIIVVGDFNSDPRD
                     PVLAGPIVPPYLQLTAAGFVDAWTQHRPGNPDGYTCCQEEDLMNRKSVLSERVDLIFS
                     SEEPEKVKVHLVGDKIPDKTPSGLWPSDHAGVVGRMEFAQ"
     gene            complement(2869..4287)
                     /locus_tag="VFK23_08780"
     CDS             complement(2869..4287)
                     /locus_tag="VFK23_08780"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HET7319216.1"
                     /translation="MALVNTNRIKPPISPLGLEYVAETLLAAGHEVTILDLCWDEQPE
                     KVIPRFFSTQEYGLVGISIRNTDDCSFATRESFLPGCARIVETIREHSGALVVAGGVG
                     FSVMPEPAMAITGVDVGVVGDGEFTMAGIANRIRGRKNWEDLPNLVIRRNGVFRRNPG
                     SAMPLSRLPPMSRALIDNRRYYLEGGQAGIETKRGCPMACVYCADPLAKGNSIRLRPP
                     GDVVAELANLIDQGIGHVHTCDSEFNVPESHALEICEGIIRRGISDRLRWYAYCKPGP
                     FSPGLARRMRRAGCEGINFGVDSGDEGMLSRLGRDFGPQEILSAARLCRENGIAVMFD
                     LLLGAPGETRESIARTIELMMRSGADRIGVTVGVRVYPGTRLAMQVMKDEQRDGLVGG
                     SDFCQPVFFLEPGIAPTAFNQLDALVGKDPRFLFFDPDRPDRNYNYNANKLLVDAIRE
                     GHRGAYWDILRRVHDQQATRPS"
     gene            complement(4451..>4685)
                     /locus_tag="VFK23_08785"
     CDS             complement(4451..>4685)
                     /locus_tag="VFK23_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319217.1"
                     /translation="FGLASRVAEAPAGINAVQFFPSMSKDRAVCLWEAKSVDALKGFL
                     DPLTAPSSRNIYYAVDSTKAMGLPKIIREEKAA"
BASE COUNT          940 a         1420 c         1290 g         1035 t
ORIGIN      
        1 tttttccttc tctttttcgc ccatcttccc cgccatgctc tcaaacatct ttttcatcct
       61 ttccatcgcg tgatcctccc ttcttccgga atccttctct gatcaagaaa ttcaaggatc
      121 aactccccat cccatggcgg atggatcgtt tcctctcctc gtactcttcc ccaccgatct
      181 ctccgagggc gtatcgtttg tcgagaatgt ccattgccga atccgttggc aataggttcc
      241 gggaatctgt gccccaagac cggaagccgc agaccccgga ccctctcctc cctcgcattc
      301 cgatacagca gaggatcatc aggaggagag gaagcaccca ccagaaggaa tgccacaaac
      361 tctcaagaac gttcatcgga atcacctcct atccgtcttt ttgtatcttg tagacggata
      421 ggaggcggaa aaggatacgc cttcgcacgg ttgttcagga attattcggc ggaatggccc
      481 gggtcgtttt tcggccatag gggaattggg actattttaa tttaatattt ccggtttatt
      541 tgaaattgat gacggccact caataccggc aggaaccgca aatcccgcgc acacccccaa
      601 cgtgacgccg atgtgcctga tcgttagtcg ttcggcattc ggctcctctg ccgggtattc
      661 cgctgttgcc gaagcgctta ctcctttttg aggatgattt tcagcaactc caaggtgttt
      721 ttctttgcat ccttcggaga ctgtttggaa gccttcgacg cttcctcaaa tgtcttgtcg
      781 gtgatgttcc tgggatcccc catgtcgtct ccgggtttcc cgcatccgca gttgtagcac
      841 atggcacctt cttcctttcg gccttgttcc cgtctcgagg ttaggacgct atggcccgaa
      901 ggaacgtttc cctttccctt ccgctccgag aacctatccg acttgcgtgg tgagatacat
      961 gggaagcgtc atgtcgccaa tctggtcctg caacgcctcg atccggtcga catgtgcatc
     1021 ctcattctgg agaatgtctt ccagtatctc ccgcgtggca aagtcatcaa catctccggc
     1081 gagcttgatg gcttgattgt aggctttgat ggcacccaac tctagcgcgt gatcgctagc
     1141 aagttgtttg gatgcatccg cgccaataga tatccgcccg agattgctga caatcggtgt
     1201 cccctcagcc tccccgccga cactgactac tgtgcgaact ccatccgacc caccactccg
     1261 gcatggtccg aaggccagag ccccgaaggc gtcttgtccg gtattttgtc gcccactaag
     1321 tggaccttga ccttttcagg ctcctcgctg gagaaaatca gatcgacacg ctcagagagg
     1381 accgacttcc gattcatgag atcctcctcc tggcagcagg tgtatccgtc ggggtttccc
     1441 ggccgatgct gggtccaggc gtccacgaat cctgccgctg tcagctgcag gtaggggggg
     1501 acgatcggac cggcgagaac cggatcacga ggatctgagt tgaagtcgcc gaccacgatg
     1561 atcggcgccc ccagctcaga gttcaaagcc gcgaggatct gagtcagctc aaaagcctgc
     1621 gccgcttgca gcaacggacc aaaatccctc ccctcgaggt gggtgttgac gaagcgtaca
     1681 gggaaaccat caaccaaggc gtcgatggct acgaagcctc tcttgatgac gatgttgtcc
     1741 aatggctcaa ggggatttgg caggcttgct gcaaattggt agttgcagcc attatccgac
     1801 acctgcgact ccgggcacag tccggtcagg acgaccacat ccttcacgtc gtcgcgcgcc
     1861 aggattacat cgcggtcgag ggcaccgatc aggttgggga caccgatccg attgatgtca
     1921 aagggaaaca ccaggtccag attcttgacg acagcaacga cacgataatt ggcaccctgc
     1981 gcctttaagg cgttcagaag ttcttgcaaa tagtcgtaaa acggcggtct cgaattagct
     2041 ccgtcgatcg tgaagttaag gacctcctgc agcccgacaa ggtgcggttt cttctccacg
     2101 atttccgcag ccaaggccgc tgcccgttcg gggaagctat tggcttcgat cgttcccagg
     2161 atatcgaaca cggccgactc gaactgtgct tcagtctgtg ccttaatcaa cgggatgagg
     2221 tcggcgccaa gataaaggtt cctggtcatc accgtcagct cctccgccgc ggcccagttg
     2281 gtcccttgga ggaccagagc cgtaagcaga atgatcacac ccttgatgca gagggtccct
     2341 ttgcctctca taacgttctc ccccatgatg ttacgttcgt gcaaacgcaa atgcggcctg
     2401 tgtttcatcg cttgggcact cgccgatagt gatacccccg gagtgatcgg acaggttgtg
     2461 tggaccgtca gggtggccga tattgacaca agtcggaaaa attgagggaa cgaggtaccg
     2521 ttctgggaaa aggaggaacc atcccagtcc accgaatcaa gagaggttaa cggttgttct
     2581 tagggttgcg gaatgtctgt cggctaattg ggttggtcta ccgggggaac gaaacgccta
     2641 tttcaatgcg tacgcccatt ccttgttcat gtcaaggata tgattcccaa cgccccccta
     2701 agcccctgaa cccgtgccgg gagtgggctg gtgaagaagt gcccggccac ccccctctat
     2761 gttatctcaa cgggtgagga tatttctggc ggagaagccg cacggactgt aaaccccgcg
     2821 caaacaaccg ctgtttccgg ggtctggaag gggcttacgc caagccgatc acgaagggcg
     2881 agttgcctgc tgatcgtgga cccttcggag aatatcccaa tatgcaccac gatgcccctc
     2941 gcggatcgcg tcgacgagga gcttgttggc gttgtagttg tagttccgat ccgggcggtc
     3001 gggatcgaag aagaggaacc gggggtcttt cccgacaagt gcgtcgagct ggttgaacgc
     3061 ggtcggcgcg attcccggtt ccaggaaaaa gaccggctgg cagaagtcgc ttccgccgac
     3121 gaggccatcc cgctgttcat ccttcatgac ctgcatcgcg agccgggtgc cgggatagac
     3181 cctcacgccg accgtgacgc cgatgcggtc ggcgcccgat cgcatcatca gctcgatcgt
     3241 ccgggcgatg ctctcccggg tttcccccgg ggcccccagc aggaggtcga acatgaccgc
     3301 aatcccgttt tcccggcata ggcgggcggc cgaaaggatc tcctgggggc cgaaatcgcg
     3361 cccgaggcgg ctcagcatcc cctcgtcccc gctgtccacc ccgaagttga tcccctcgca
     3421 gccggctcgg cgcatccgcc gggcaagccc gggggaaaag ggccccggct tgcagtaggc
     3481 gtaccagcga agtcggtcgc tgatccctct gcggatgatc ccctcgcaga tttcgagggc
     3541 gtggctttcc ggaacgttga actcgctgtc gcaggtgtgc acgtgcccga tcccctggtc
     3601 aatcaggttg gccagctccg ctacaacatc cccgggaggt cgaaggcgga tcgagttacc
     3661 ctttgcgagc gggtccgcgc agtagacgca cgccatcggg catccccgct tcgtttcaat
     3721 ccccgcctgc cccccttcaa gataataccg gcggttgtcg ataagcgccc ggctcatcgg
     3781 ggggagccgg gagaggggca tggcggaacc tgggttgcgg cggaagaccc cgttccggcg
     3841 tattacgagg ttcggaagat cctcccagtt tttccgtccc cttatcctgt tcgcgatccc
     3901 tgccatggtg aactcaccgt ccccgacgac gccgacgtcc acccccgtga tggccatggc
     3961 gggctccggc atcacggaga atccgacacc ccccgcgacg accagagcgc cggaatgttc
     4021 ccggatcgtc tcaacgatcc ttgcgcaacc gggaaggaac gactcccgcg tggcgaaaga
     4081 gcaatcatcc gtgttacgga tcgatattcc gaccagaccg tactcctggg tgctgaagaa
     4141 ccttgggatc accttttccg gttgctcatc ccagcaaaga tcgaggatgg tcacctcgtg
     4201 tccggcggca agcagcgtct cggcgacgta ttcaagcccg agaggagaaa tggggggctt
     4261 gatcctgttc gtgttgacga gtgccactcg catcctttca taataaggga aatcggcggg
     4321 tactgaccca gttttccccc cccgggcata ttgggtcagg gtcgacgcat cccgatttca
     4381 ttctgagaga agggcccggg cagaatcccg ggccctctca ggacaatggg gttgtgttga
     4441 ccggcctctc ctacgccgct ttctcctccc ggatgatctt cggaagaccc atcgcttttg
     4501 tgctatccac cgcgtagtag atgttacgac tgctcggagc agtcaaggga tccaagaagc
     4561 ccttcaaagc gtctaccgac ttggcttccc aaagacagac cgcccgatct ttgctcatac
     4621 tcgggaaaaa ctggactgca ttaattcccg caggtgcttc ggcgactctt gaggcaagcc
     4681 caaaa
//
LOCUS       DASSLD010000488         4085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_786508,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000488 DASSLD010000000
VERSION     DASSLD010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786508"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VFK23_08790"
     CDS             <1..913
                     /locus_tag="VFK23_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET7319218.1"
                     /translation="TGDIVLAVGTPFGLNSTVTMGIVSAVGRSNVGIADFEDFIQTDA
                     AINPGNSGGALVNTDGELVGINTAIFSTSGGYMGIGFAIPADMVKSVMDSIIKHGKVI
                     RGWLGVTIQNLTADLAKSLNIKETPGALVSGVEKDSPADKAGLKRGDLIVELNGKKVD
                     DSTMLRNMVSQSAPGTKADFKIMREGKEKTVTATLGEYKEKQVVKKAEYDNVLRGVTV
                     QELTPGIRDKLNLPENMNGVVITGVGPDSPAQGILQPNDVIQEVNRQPIQGAQDFDGA
                     VSKIGAADTVLLLIYRDGGSIYLTIRP"
     gene            1084..1299
                     /locus_tag="VFK23_08795"
     CDS             1084..1299
                     /locus_tag="VFK23_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319219.1"
                     /translation="MRLRISPDMDFYLDMELQGVTEKTRDYDIQQFEKVVFDEFVSRL
                     KKAFPEGDFRVYTFEFNVARESSKKAA"
     gene            complement(1403..1807)
                     /locus_tag="VFK23_08800"
     CDS             complement(1403..1807)
                     /locus_tag="VFK23_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319220.1"
                     /translation="MIQNKIVIQYLNGSVMKGSTRDFMPNKEVFHLTPVDAPQDCKPV
                     AVVVRELKAVFFVKDHAGNSQYQEKKEFERNAPALGKKIKVIFRDGEILIGTTQGYQP
                     GRAGFFIIPADPKSNNDRCYIVSSSTKEVSFI"
     gene            complement(1915..3324)
                     /locus_tag="VFK23_08805"
     CDS             complement(1915..3324)
                     /locus_tag="VFK23_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319221.1"
                     /translation="MSVSYTIKRLYDVAFAILYQKRLDRHDRWSREKLDKHQRSRLSS
                     LVSYAVRHSPFYKKHYGHFVDGKEVVLRDLPVVNKAVMMENFDAFVTDPRLKLAELQS
                     HIASLDRDDAYHLGEYRVLTTSGSSGRKGVFVFNRKEWSTAIAGYWRCGAFMGIKPRL
                     PRWKMAIVAAGSPMHVSYRSSVSSDVGIMKVKRLEATACLQDLVRDLNEFQPECLAGY
                     PSVISLLADDQIEGRLQIRPRVISTTGELRTEDMEQKMRKAWGTVPYNLYGMTESGVF
                     CGSDCSLHRGIHIFEDLFIVEAVDERNRPVHDGALSTKLLITNLFNYTQPLIRYEVSD
                     MVSMTGDACPCGRPFRRIVSMEGRSDDILYFPGSAGCDIAVHPLNFRSPMASFPDILE
                     YRIVQERDCVDVSLVLRQGTPADQVAERVKKKLREKVETLGARCPELRIRQVPCIERD
                     TQKMGKLKIVQSNIKKAGK"
     gene            complement(3561..3965)
                     /locus_tag="VFK23_08810"
     CDS             complement(3561..3965)
                     /locus_tag="VFK23_08810"
                     /inference="COORDINATES: protein motif:HMM:NF022317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 family protein"
                     /protein_id="HET7319222.1"
                     /translation="MKTADAPMKRAALLLLDDLFHALHLGIIIFFLFGWTIRETRPAH
                     YALPLLILLSWYGLGAFRGFGYCIITDIQWSIKKRLGNKPSTEYYIKYLIDNITGLDT
                     RANIVNGVTTYTYFIILILSTILLLVSNIHYE"
BASE COUNT          948 a         1092 c         1119 g          926 t
ORIGIN      
        1 aacgggagat atcgtgctag ccgtaggcac gcccttcgga ctgaactcga ccgtcaccat
       61 ggggatcgtg agtgccgtcg gcaggtcgaa cgtgggaatc gcggacttcg aggatttcat
      121 ccagaccgat gcggcgatca accccggcaa ctcgggcggc gccctggtaa acaccgatgg
      181 tgaacttgtc ggcatcaaca ccgcgatctt ttcgaccagc ggcggataca tggggatcgg
      241 ttttgccata ccagccgaca tggtgaagtc ggtcatggac agcatcatca agcacggcaa
      301 ggtaatacgt ggctggctgg gagtgaccat ccaaaacctc acggccgacc tggcgaaatc
      361 gctgaacatc aaggagaccc ccggcgccct cgtatccggc gtcgaaaaag acagcccggc
      421 ggacaaagcg gggctcaagc ggggcgacct catcgtcgag ctgaacggca agaaagtcga
      481 cgattcgaca atgcttcgga acatggtatc ccagtcggcc cccggaacca aagcggattt
      541 caagatcatg agagagggca aagagaagac cgtgacggcc acactgggag aatacaagga
      601 gaaacaggtt gtaaaaaaag cggagtatga caatgtcctc cggggcgtta cggtccagga
      661 actcaccccg gggatccggg acaagctgaa tctgccggag aacatgaacg gagttgtcat
      721 caccggggtc ggtccggaca gcccggccca gggtatcctc cagccgaacg acgtgatcca
      781 ggaggtgaat cgccagccca ttcagggggc tcaggatttc gacggggctg tttcgaagat
      841 aggtgcggcg gacaccgtgc ttttactgat ctaccgcgac ggcgggtcga tctaccttac
      901 gatacggccg tagcacgaac ggagcggtga ggagaggtat aatttagtta gaaaaggtac
      961 aaagcaatgt attttaatgt gtcattcctg cggaagcagg aatccattct tcgtctcaac
     1021 agtcaatgga ttccgggtca agcccggaat gacggagtgg gttggaaaga gaggtgaacc
     1081 tctatgcggt tacgcatttc accagacatg gacttctacc ttgacatgga acttcagggt
     1141 gtgacggaaa agacgaggga ctatgacatc cagcagttcg agaaggtggt cttcgacgag
     1201 tttgtgagca ggctcaaaaa ggcgttcccg gaaggggact tcagggtcta taccttcgag
     1261 ttcaacgtcg cgcgggaatc atcgaagaag gcggcgtaaa agacttcagt tcggtgttca
     1321 tggttcggaa ttccaaagaa agaagcgttc tttgatgtta actctgaacc atgaacgctg
     1381 aacatacgat tttgccttcc gcttaaatga atgaaacttc cttcgtcgac gaggagacga
     1441 tatagcagcg atcattgttg gatttgggat cggccgggat gataaaaaaa ccggcgcgtc
     1501 ccggctgata gccttgggtc gtaccgatca gtatttcccc atcccgaaaa attaccttga
     1561 tttttttccc gagagcgggc gcgttccttt caaattcttt tttctcctga tactggctgt
     1621 tccccgcatg atccttcaca aaaaacaccg cttttaattc cctgacgaca acggctaccg
     1681 gcttacaatc ttgcggggca tctacgggtg taagatgaaa gacttccttg ttgggcatga
     1741 aatccctggt gcttcccttc atcacgctgc cgttcaggta ctgaattacg atcttgtttt
     1801 gtatcatggc ctgtcctcgt gaagtcatgc cgtcccgccg gcacgaaact tgaacatttt
     1861 tttcgaaggc ttcgattata tcatcatccg gtcgctttca caagggccgg cgtatcactt
     1921 gccggccttc tttatgtttg actgaactat cttgagtttc cccatctttt gcgtgtcgcg
     1981 ttcaatacag gggacttgcc ggatacgcag ttcaggacac cgggcgccga gcgtctcgac
     2041 tttctctctc agtttctttt tgaccctctc ggccacctgg tccgccggcg ttccctgccg
     2101 caggaccagg gacacgtcaa cgcagtctct ttcctggacg atgcggtatt ccaaaatgtc
     2161 agggaatgaa gccatcggac ttctgaaatt cagcggatgg acggcaatat cgcacccggc
     2221 agagccgggg aagtagagaa tgtcgtcgct tcttccttcc atgctgacga tgcgcctgaa
     2281 cggccggccg cagggacagg catcccccgt catgctgacc atgtctgaaa cttcgtaacg
     2341 aatgaggggc tgtgtatagt tgaacagatt cgtgatcagg agcttggtac tcagggcgcc
     2401 gtcgtgcacg ggcctgtttc tttcatccac ggcctcgacg atgaaaaggt cctcgaaaat
     2461 atggatcccc cggtgaaggg agcagtcgga cccgcagaat actcccgact cggtcatccc
     2521 gtacaaatta tagggaacgg tcccccaggc cttccgcatt ttctgttcca tgtcttccgt
     2581 gcgaagttcg cccgtcgtcg atattacccg gggtcggatc tgcagccgcc cctcgatctg
     2641 gtcatccgcg agaagcgata tgaccgatgg atatccggcc aggcactccg gttgaaattc
     2701 gtttaaatcg cggacaaggt cctgtaggca ggcggttgct tcgaggcgct tgaccttcat
     2761 aatcccgacg tcgctgctga cggaggaacg gtagctgacg tgcatcgggc ttcccgctgc
     2821 gactatcgcc atcttccatc ggggcagccg gggcttgatg cccatgaacg caccgcaccg
     2881 ccaatatccc gcaatggcgg tgctccattc ttttctgttg aagacgaata cgcccttgcg
     2941 ccccgaactc ccgctcgtgg tgaggacccg gtattctccc agatgatatg cgtcgtcccg
     3001 gtccaggctt gcgatgtggg actgaagttc cgccagtttg aggcggggat cggtgacgaa
     3061 agcgtcaaaa ttttccatca tgacggcttt attgacgacc ggcaggtccc gaagcacaac
     3121 ctcttttccg tcaacgaaat gaccataatg ttttttgtag aagggtgagt gccggaccgc
     3181 gtaggaaacc agggaggaaa gccgggaccg ctgatgtttg tccagttttt ccctggacca
     3241 ccgatcatgc cggtcgaggc gcttttgata caaaatggcg aaagcgacgt cgtacaggcg
     3301 ctttatcgta tagctcacgc tcatggcttg cctcctgttg ccatacttga caaaaacggc
     3361 actcacggca ggcacgcggc tcccgcctga ttcacggggg cgcaatcgat gcgcgtacat
     3421 acgtattgtg cattattacc gagctctcgt caagattgaa accccctgca atcccgtcgc
     3481 cccgcagccg catgctctgc gcaaactact tgcgccgctc acctacattg gcagtcacgg
     3541 agtaatcagg tagcaaaata ctactcgtaa tgaatgttgc tcaccagcag cagaatagtc
     3601 gaaaggatga gaataatgaa ataggtgtaa gtcgtcacgc cgttgacgat gttcgcgcgg
     3661 gtatcgagcc ccgtaatgtt gtcaatcagg tatttaatat agtattcggt ggacggcttg
     3721 ttgcccaacc gtttttttat gctccattgg atatcggtga tgatgcaata tccgaacccc
     3781 cggaatgccc ccaatccata ccaagacagc agtatcagga gcggaagcgc ataatgggcc
     3841 ggtctcgttt cccttatcgt ccatccaaaa aggaagaaga tgattatgcc caggtgaagc
     3901 gcatgaaaca ggtcatccag gagaagcaat gccgctctct tcatgggtgc atcagccgtt
     3961 ttcaacaatc gcctccgcta tctcttcgga atacgacgtg accttctcaa aagctcggac
     4021 gtactggtcc aggatgccgt tgtcaggccc cgggaaagac ggcagtttca gcagggtctt
     4081 attgg
//
LOCUS       DASSLD010000489         6635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_921919,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000489 DASSLD010000000
VERSION     DASSLD010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921919"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1379)
                     /gene="rsxC"
                     /locus_tag="VFK23_08815"
     CDS             complement(45..1379)
                     /gene="rsxC"
                     /locus_tag="VFK23_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HET7319223.1"
                     /translation="MEATTVQAKNTFSGGVHPADNKQLTAHKPTVAAAIPKRAVIPLS
                     QHIGAPTKALVVIGQEVKKGEKIGETTGFVSAPVHASVSGKVVALGNFPHILGADLPS
                     VVIESNGKDEWVGGLKETPDYMTLSPDELKKMVADAGIVGMGGATFPTHVKLSPPKEK
                     PIDVVILNGAECEPYLTSDHRLMLEKSAEIVEGLKILMRILNVSRGHIGIESNKPDAI
                     EAMTRAAAGSPEIKVWPLKVKYPQGAEKMLIKAIAGRTVPAGGLPMDVGVVVQNVGTA
                     EAIYQAVRFGRPLIERYVTVTGRGVKEPKNFLARIGTPFSQLIEEAGGLTDTAAKVIS
                     GGPMMGMSQYTLDVPVIKGTSGITVLPQNEVSTKSYGPCIRCGRCIDACPMKLQPSYI
                     GLYIEKGHYQDAKDYNLMDCFECGSCTFVCPANRPMVQWVKKAKKELAKKKN"
     gene            complement(1750..1977)
                     /locus_tag="VFK23_08820"
     CDS             complement(1750..1977)
                     /locus_tag="VFK23_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET7319224.1"
                     /translation="MEKEKVQEALNKVRPMLQRDGGDVELVSVDANGSVKVRLKGACG
                     SCPMSTMTLKMGVEKFLKEQIPEVKEVIQVA"
     gene            2126..2533
                     /gene="crcB"
                     /locus_tag="VFK23_08825"
     CDS             2126..2533
                     /gene="crcB"
                     /locus_tag="VFK23_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014182962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET7319225.1"
                     /translation="MNTRVEGLKHRMANIVIIGIGGFIGAVARYGIALWIGQRWGRSF
                     PLGTFIINVTGSFLIGLLMTVMAERFTENPQWRLFLVVGFLGAYTTFSTFEYETGALL
                     KDSEWLYAGLNVVLSVAVGFVALKLGEVIAKSI"
     gene            2682..3020
                     /locus_tag="VFK23_08830"
     CDS             2682..3020
                     /locus_tag="VFK23_08830"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HET7319226.1"
                     /translation="MVKSGPAKKLTIYVDEADKFNGKPVYEVLLDIFFKRKISGASVF
                     RGIEGYGSDGVFHTVKILELSTALPVKIEVVDSQDMINDVLPEITSIVEKGLIEVSDT
                     NVIKCCMRGK"
     gene            3057..3653
                     /locus_tag="VFK23_08835"
     CDS             3057..3653
                     /locus_tag="VFK23_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7319227.1"
                     /translation="MTIQSLDTQFLLLINHGMANAFLDILMPALSLRGYLLVIPYILA
                     MLLISANRRDREGRTFISTTVWALVIAFGAWYLSEWAEDALKVAIARERPCRELEHIR
                     LILPCPKSFSMPSGHAITSFAVAAPLFYLPRAYIALIWRLYPVVLASFIAFSRLYLGV
                     HYPTDVLAGTFLGILIGLAPSILYQVLATEEVVKRRKA"
     gene            complement(3701..4342)
                     /locus_tag="VFK23_08840"
     CDS             complement(3701..4342)
                     /locus_tag="VFK23_08840"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HET7319228.1"
                     /translation="MNYEKERFRMVDEQILCRGVRDERVIAAMRRVPRHEFLPEAIRG
                     MAYQDSALPLGEGQTMSQPYMVACMTELLGLQGTERVLEVGTGSGYQAAVLSELCAKV
                     YSVERIKLLAERARLNLDRLGYRSVAIKVYDGTYGWKEMSPFDAIMVTAGAPDVPAPL
                     VDQLKEGGRMVIPVGDRYGQVLLTIVKKPSGKVTERSIPCTFVPLVGNHGWKE"
     gene            complement(4342..5118)
                     /gene="surE"
                     /locus_tag="VFK23_08845"
     CDS             complement(4342..5118)
                     /gene="surE"
                     /locus_tag="VFK23_08845"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET7319229.1"
                     /translation="MILVTNDDGVYSPGIQILAKRLRELDDVVIVAPDRERSAAGHSM
                     TLHRPLLIEEVRDAMYSVNGTPTDCINIAVKGLLKETPRLVVSGINKGPNLGDDVTYS
                     GTVAGAMEGILLGIPSFAVSLAAREDFLFAEAAEVALRTADSIYKQGLPAGTLLNINV
                     PNLPVSEIQGTRITRLGKRIYHQMTVERVDPRGKKYYWIGGGEPDWEREEGTDLDAID
                     RKFVSITPLHLDFTDYSSFKRLQSLQEMRFTPPVAHRSSE"
     gene            5469..5903
                     /locus_tag="VFK23_08850"
     CDS             5469..5903
                     /locus_tag="VFK23_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319230.1"
                     /translation="MKKIMFLSMMMVLALTAGTIYADESGSLPYNGITVFSTGPVAFD
                     DVPLLAGTMNWSYEEGSAAGGWREEEAMTETHPYNGVTIFSDRAIDFDSVPLGAVAQI
                     GTYEEGTAAGGLHEEEAMALPENGITIFSKEPVAYDSLPVRW"
     gene            complement(6026..6598)
                     /locus_tag="VFK23_08855"
     CDS             complement(6026..6598)
                     /locus_tag="VFK23_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7319231.1"
                     /translation="MKRFIIITLAAFLTLGMTALAFARTDRSTSDDKEIDGSVVLATA
                     PASGFDTGFGITVGGGAMLPQIDRNLQGRIDISYFVWSASEFGVDVTYTRVPILFSGR
                     YFVPARDSRLKVYIQGGLELSFDTVEVAVPVPFRGTVKESSNETRIGLVPGAGIEYKL
                     SPTIGLVADARLHIITDSYLTLQFGLASHF"
BASE COUNT         1414 a         1812 c         1813 g         1596 t
ORIGIN      
        1 ggactgctgt ctttttctcc gaactccgag ctgcaaactc cgaactaatt cttctttttc
       61 gccagttctt tctttgcctt cttcacccac tggaccatgg gccggttggc ggggcagacg
      121 aatgtgcagg agccgcattc aaaacagtcc atgagattgt agtccttggc atcctggtaa
      181 tgccccttct cgatgtagag tccgatgtag gaaggttgga gtttcatagg acaggcatcg
      241 atgcaccggc cgcagcggat gcagggcccg taggatttcg tgctcacctc gttctgcggc
      301 agaaccgtga tgccggaggt acccttgatc accgggacgt cgagcgtata ctggctcatg
      361 cccatcatcg ggccgccgga gatcaccttc gccgccgtgt cggtgaggcc gcccgcctcc
      421 tcgatgagct gggagaacgg cgtgccgatc ctggccagga agttcttcgg ttcttttact
      481 cctcttccgg tcacggtcac gtagcgttcg atgagcggcc ttccgaaacg aacagcctgg
      541 tagatggcct cggccgtgcc cacgttctgc accaccacgc ccacgtccat gggcaggccc
      601 cccgcaggca ccgttctgcc ggcgatggcc ttgatgagca tcttttccgc gccctgcggg
      661 tatttgacct tgaggggcca caccttgatc tcaggagagc cggcggccgc cctggtcatg
      721 gcctcgatgg cgtccggctt gttcgactcg atgccgatat ggcccctgct cacattcaga
      781 atccgcatga ggatcttgag gccctcgacg atctcggctg acttttcgag catgagtcgg
      841 tggtcggagg tgaggtaagg ttcgcactcc gctccgttca ggatcacgac gtcgatgggc
      901 ttctccttgg gaggagagag cttgacgtga gttgggaacg tggccccgcc catgcccacg
      961 atccccgcgt cggcaaccat cttcttgagt tcgtccgggg aaagcgtcat gtaatcgggg
     1021 gtttccttaa ggccaccgac ccactcatcc ttgccgttgc tctctatgac gaccgagggg
     1081 aggtccgcgc cgaggatgtg cgggaaattg ccgagagcga ccactttgcc cgagaccgag
     1141 gcgtggacgg gagcggaaac gaaaccggtc gtttctccga tcttctctcc ttttttcact
     1201 tcctgtccga ttacgacgag ggccttggtg ggtgctccga tgtgctggct taaggggatg
     1261 actgctcttt tggggatagc tgcggctacg gtgggtttgt gtgcggtaag ctgtttgtta
     1321 tcagccggat gcacacctcc gctgaacgtg ttcttcgctt gtacggtcgt ggcttccata
     1381 atctgtttca cctgccgtct ttatgtaatg aacgatggga actgcagctt gagattttct
     1441 aaaaaccgcc ccgtttataa cagaaagggc accgatgtgt caagcgaatt ttcgccattt
     1501 tacacgtcaa aaccctgttt gttacaattc gaaacactgg gtgcgggcga acgctcttgg
     1561 gcatccgttt actgtcattg atgaggcggg ggagagcgca aaaccccgtg cgcaatgatg
     1621 aagatctaag gcgagaaata gctcgtcgtc cccgaatgcc tttatcggaa acaggcgtat
     1681 tttctaaatc ccggttccct gataaggcat tcgggaatga cggctaaaaa aagcgattac
     1741 cgtgtctggt catgccacct ggatcacttc cttgacttcg ggtatctgtt ccttcaaaaa
     1801 cttttcgact cccatcttga gcgtcatcgt cgacatgggg cacgaaccgc aggccccttt
     1861 cagccggact ttgaccgatc cgttcgcatc gacgcttacc agttccacat cgccgccatc
     1921 ccgctgaagc attggtcgca ccttgttgag cgcttcctgg actttttcct tttccatgat
     1981 cgttctcctt ctcccgtttt ggcatcttgc tgtaaaacca tcttattata caccttttta
     2041 ccagcggtga acattttttt ctatgataga ggtcatatcc cggccggaag taccatttcg
     2101 attgacttgc ccggcgcctt ctgatatgaa tacgcgagtt gagggattga aacaccgcat
     2161 ggcgaacatt gtcatcatag gaatcggtgg attcatcgga gccgtagcgc gctatggaat
     2221 agcgctctgg atcggccaac gctggggcag gagcttcccg ctcgggacct ttatcatcaa
     2281 cgtgacgggc agcttcctga tcggcctgct gatgaccgtg atggcggagc gcttcaccga
     2341 aaacccgcag tggaggcttt ttctcgtggt aggcttcctc ggggcctaca ccacattttc
     2401 cacgtttgaa tacgagaccg gcgcgctcct caaagacagc gagtggctct acgccgggct
     2461 gaacgtggtc ctgagtgtgg ccgtcggatt cgtcgcgttg aagctcggcg aggtaattgc
     2521 gaagagcata taaggagaaa attccaaata taaaatttca aatcaaaaat aaattccaat
     2581 attcagattg caaaattggt atctagtttt attggagttt gtttggtatt tggtgcttga
     2641 aatttgggat ttctctcctt cttgttcaca aggagagtgt catggtcaaa agcggacccg
     2701 cgaaaaagct taccatctac gttgatgagg cggacaagtt caacggcaag ccggtctacg
     2761 aggtactgct ggatatattc ttcaaaagga agatctccgg cgccagcgtg ttccgcggca
     2821 tcgagggcta cggcagcgac ggagtatttc acacggtaaa gattctcgaa ctctccacag
     2881 ccctgccggt gaagatcgag gtcgtggatt cgcaagacat gatcaatgat gtcctgcctg
     2941 agatcacctc gattgtcgaa aagggactca tcgaggtgag tgacacgaac gtgatcaagt
     3001 gctgcatgcg ggggaaatag atccctttca cgatgtaaat cttctcgata gattccatga
     3061 cgatccagtc cctcgacacc cagtttctcc tcttgatcaa ccacggcatg gcgaatgcct
     3121 ttctcgacat tctgatgccg gcgctttcgc tgcgcggata ccttttggtc attccctata
     3181 tcctcgccat gctcctcatc agcgcgaaca gaagagaccg ggaaggcagg accttcattt
     3241 ccacgacggt ctgggccctc gtgatcgcct tcggcgcttg gtacctgtcc gaatgggccg
     3301 aggacgcact taaagtcgcc attgcaagag agaggccgtg ccgcgagctg gagcacatca
     3361 ggctcattct tccctgcccc aagtccttct ccatgccctc aggccatgca atcacttcct
     3421 ttgccgttgc agcgccactt ttctatttgc cccgggcata catcgcatta atatggcgtc
     3481 tgtatcccgt ggttctggcg tcgttcatcg ctttttcgcg gctatacctc ggtgtccatt
     3541 atccgacgga tgttctggca gggacgtttc tggggattct catagggctg gcgccgtcga
     3601 tactgtatca ggtgctagca accgaagagg ttgtgaagcg aagaaaagct tgatggggaa
     3661 gttcgttgtt ctcactctga actctgaact ccgaactgtt ttactctttc cacccatggt
     3721 tgccgacgag cggcacgaaa gtgcacggaa tgctccgctc cgtaaccttc cctgacggct
     3781 ttttgacgat tgtcagcagg acctggccgt agcggtctcc caccgggatc accattctgc
     3841 cgccttcctt gagctgatcc acgagaggcg cgggcacatc cggagctccc gccgtaacca
     3901 tgatggcgtc aaaaggcgac atctccttcc agccgtacgt cccgtcatag accttgatcg
     3961 cgacgctccg atagcccaga cggtccaggt tcagccgggc ccgttccgcg agcagcttga
     4021 tgcgctctac cgaatagacc tttgcgcaca gttcggaaag gacggctgcc tggtaacccg
     4081 agccggtccc gacctcgagg actctttctg tgccctggag cccgagaagc tcggtcatac
     4141 acgccaccat gtagggctgg gacatggtct gcccctcccc cagcggcagc gcgctgtcct
     4201 ggtacgccat gccgcggatg gcctccggaa ggaactcatg ccgggggacc ctgcgcattg
     4261 ctgcaatcac gcgttcgtcc ctgactcccc gacacaggat ctgctcgtcc accatacgga
     4321 agcgttcttt ttcgtagttg atcactcgga gctccggtga gcaacaggcg gtgtaaaacg
     4381 catttcctgc agagactgca gccgcttgaa ggaagaatag tccgtaaagt ccagatgaag
     4441 gggcgtgatg gagacgaact tccggtcaat cgcgtcgagg tctgtgccct cttcccgctc
     4501 ccagtccggt tctcccccgc cgatccagta gtacttcttg ccgcgagggt caacgcgctc
     4561 gacagtcatc tggtggtaga tgcgcttgcc cagccgggtg atgcgcgtgc cctgtatttc
     4621 ggacaccggg aggttcggga cattgatgtt gaggagcgtc cccgcgggaa gcccttgttt
     4681 gtatatgctg tctgccgtac ggagcgccac ctcggctgct tcggcgaaaa gaaaatcctc
     4741 ccgggcggcg agtgaaacgg caaaggaggg tatcccgagc aggatgcctt ccatcgcgcc
     4801 tgcaacggtg ccggaatacg tgacgtcgtc accaaggttc ggccccttgt tgatgccgga
     4861 aaccacgaga cggggcgttt ccttaagaag ccctttgacc gcgatattga tacagtccgt
     4921 cggggtcccg ttaacgctat acatcgcatc cctcacctct tcgatcagga gcggccggtg
     4981 gagtgtcatg gagtggccgg cagcgctccg ctcgcggtcc ggcgcaacga tcacgacgtc
     5041 gtccagttca cgaagcctct ttgccaggat ctggatgccc ggcgaataga cgccgtcgtc
     5101 attggttaca agaatcatga aaaaatctcc ttctcggcgg ccaccgattt tctcatccat
     5161 ccgccgcaac gcggcattgt atatcaaaat acgggagctt tgcaaaggtt ttttcggtaa
     5221 cgggtgccgg gataatgcag tgcgcgttca tggattattg cccgggataa ctacgttccc
     5281 tgtaatgata caaggggata tgcctgtcat gtaataaaaa agaatgagga tgaatcaagt
     5341 ctgctttatg cataaatcat tccatagtcg ccttaaaaat ggattgacat acgtgatata
     5401 atactaacag gatataattt atcctgattt aagtcgggat cgaatgaata cgaaatgagg
     5461 tgaccaaaat gaaaaagata atgttccttt caatgatgat ggttcttgcc ctcaccgccg
     5521 ggactatata tgcggatgag agcggcagcc tgccttacaa cggaattacg gtgttcagca
     5581 ccggcccggt tgcattcgac gatgttccgc ttctggcggg taccatgaac tggtcctatg
     5641 aagagggttc agcggctggc ggttggcgtg aggaagaagc aatgacggag acacatcctt
     5701 ataacggcgt cacgattttc agcgaccgtg ccattgactt cgattcggtg ccgctgggag
     5761 cggtagcaca aatcggcact tatgaagaag gtacagcagc aggcggtctg catgaggaag
     5821 aagccatggc attaccggag aacggcatca cgattttcag caaagagccg gtggcatatg
     5881 acagtcttcc ggttcgctgg tagtaaacca agcccggagc tcttatgaac agagcaacgg
     5941 ttccatgaaa cataacatga agaacaagaa aagccccgat aattgtcggg gcttttcttt
     6001 aatgcaatca ttctcaaagg tcagcctaga aatgtgacgc caggccgaat tgcagggtca
     6061 gatagctgtc cgtaatgata tgcaaccggg cgtcggcgac aaggccgata gtgggtgaaa
     6121 gtttgtattc gatgccggcg ccggggacaa gaccgatgcg ggtctcgttg gatgattctt
     6181 taacggtacc acgaaacggg acgggaaccg cgacctccac cgtgtcaaaa ctcagttcaa
     6241 gaccaccctg aatgtagacc ttcaacctgc tgtcacgcgc ggggacgaaa tatctcccgc
     6301 tgaataagat ggggacgcgc gtgtaggtca catctactcc gaactcggag gcactccata
     6361 cgaagtagga aatatcgatc ctgccttgaa ggtttctgtc gatttgggga agcatggcgc
     6421 ccccgccgac cgtaataccg aaacccgtgt cgaaacccga tgccggggct gtggccagga
     6481 ctaccgaccc gtctatttcc ttgtcatcag atgttgacct gtcggttcta gcaaaggcaa
     6541 gggctgtcat accgagtgta aggaatgccg cgagcgtgat gattatgaat cttttcatct
     6601 ctttcctcct gatggatttt gttttttaat tttct
//
LOCUS       DASSLD010000490         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_668913,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000490 DASSLD010000000
VERSION     DASSLD010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668913"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VFK23_08860"
     CDS             <1..509
                     /locus_tag="VFK23_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HET7319232.1"
                     /translation="LSRDGTAEIYTMGRDGSGLERLTYGASDNVSPSWAPNGRDIVFT
                     SGRAGTPQLYVMGADGTDVRRITSAGNYNASPNWSPRGDKIVFASQVKGIFKIATINP
                     DGSDFRILTSGPGSDENPSWSPSGRQIVFSSNREGKSNIYIMNANGTDIERITKNDAN
                     YTSPAWSP"
     gene            604..>2315
                     /locus_tag="VFK23_08865"
     CDS             604..>2315
                     /locus_tag="VFK23_08865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7319233.1"
                     /translation="MLISGVLTTAFYVYSHRAVDKQTTENILQQFQTISYHFRYELRD
                     ALVKDLQLLASNPALDGFITGKGGREIEVSEVERFFLQSLKYSRSYESITFVDRAGSE
                     VIKVNWSGRVRTYRDAAGRPLFHALKNGLPGGIDVEGPVVAPYGEVLFSAGIHKTGAR
                     LGDFSGAVIIDHSLKDFIDYLDKIRIFDVNPLWLFLPDGTVIKQPNDKRAFLDPRPYL
                     AAGFHKEPVLAPVENGMLVYQDLYINPDHPLLRLAISIPSSLLLEDMRRVLRFFLIVS
                     AVSLLFISVLAYYLAGYLSRPIIGFAEAASRLAKGDLAARVPGTSTGEVGLLIDSFNR
                     MGENLQKTTVSKENMDNIIGSMMDTLIVVSPEGLIERLNQAACILLGYEEGELIGRPV
                     STVISDGLQGEAELDDILSRGSTSTVEKSYITKNGANVPVLFSAAVMRNAGGAVRGVV
                     CVAKDITDRKHMEERLQRLALYDTLTGLPNRALFFDRANHLLAAARRNRHLLALLYMD
                     LDRFKTVNDGLGHEMGDLLLAEAARRIASCTRQEDTVARMGGDEFIGLCGKIVSPEDA
                     AVVAR"
BASE COUNT          465 a          722 c          690 g          438 t
ORIGIN      
        1 cgctctcccg ggacggcact gccgagatct acaccatggg cagagacggc agcggcctcg
       61 agaggctgac gtacggcgcg tccgataatg tgtcgccttc atgggcgccg aacggcaggg
      121 atatcgtctt cacctcgggc agggcaggga ccccgcaact gtacgtcatg ggcgccgacg
      181 ggaccgatgt ccgacgcatc acctctgcag ggaactacaa cgcttccccg aactggtcgc
      241 cccggggaga taagatcgta ttcgcctccc aggtcaaagg gatcttcaag atcgcgacca
      301 taaaccccga cggaagcgat ttccgcatcc tgaccagcgg gccggggagc gacgagaacc
      361 cgtcgtggtc gccgtcgggc agacagatcg tgttcagctc gaaccgcgag gggaagtcca
      421 acatttatat catgaacgcc aacggcaccg atatcgagcg gatcacgaaa aacgacgcca
      481 attacacatc gcccgcctgg tcgccgtgag cgaaaactat tcatcaccct cttattgatg
      541 aagacatcga gcgccggact ttccatattt tcacgccttt tgatcgcgct tctgagcgta
      601 gtcatgctca tcagcggcgt gctgacgact gccttctacg tctacagcca tcgcgccgtg
      661 gataagcaga cgacggagaa catccttcag cagttccaga ccatcagcta ccacttccgt
      721 tatgagctcc gggacgccct ggtgaaggac ctccaactcc tggcctccaa tcccgcgctg
      781 gacggtttca tcaccggcaa gggggggcgt gaaatcgagg tctcggaggt ggaacgcttc
      841 ttcctgcaat cgctcaagta cagcaggagc tatgagagca tcaccttcgt cgatcgcgcg
      901 ggcagcgagg tcatcaaggt caactggtcc ggacgggtcc gcacgtaccg tgatgccgcg
      961 ggacgtccgc ttttccacgc gctcaagaac ggactgcccg gcggcatcga tgtcgagggg
     1021 ccggtcgttg ctccgtacgg ggaagtcctg ttctctgccg gcatccacaa gacgggtgcg
     1081 agactcggcg atttcagcgg agccgtcatc atcgaccaca gcctgaagga tttcatcgac
     1141 tacctggaca agatcaggat tttcgatgtc aatccgctct ggctttttct gccggacggt
     1201 acggtcataa agcagccgaa cgacaagcgc gcgttcctcg acccccggcc ctacctcgcg
     1261 gcaggtttcc acaaagagcc cgtcctggcg ccggttgaga acggcatgct ggtctaccag
     1321 gacctctata tcaatccgga ccatccgctc ctcaggctcg caatcagtat cccgtcctcg
     1381 ctcctgctcg aggacatgag gagggtgctg cggtttttcc tgatcgtatc ggccgtttcc
     1441 ctgcttttca tttccgttct tgcctattac cttgccggct acctgtcccg cccgatcatc
     1501 gggtttgcgg aggccgcttc acgtctcgca aagggcgacc ttgccgcgcg cgtgccgggg
     1561 acgtcgacag gcgaggtggg gctcctgatc gacagcttca accgcatggg tgagaacctc
     1621 cagaagacga cggtgtccaa agagaacatg gacaacatca tcggcagcat gatggacacg
     1681 ctcatcgtcg tgtctccgga gggcctgatc gagcgcttga accaggcggc ctgcattctt
     1741 ctgggatacg aggaggggga actgatcggc aggcccgtct ccacggtgat ctccgacggg
     1801 ctgcaagggg aggcggaatt ggacgacatc ctctcgcggg gctcgaccag cacggttgaa
     1861 aaaagctaca tcacgaagaa cggggcaaac gtgccggttc tcttctctgc cgcggtcatg
     1921 cgcaacgcgg gaggcgcggt ccggggcgtc gtctgcgtcg ccaaggacat cacggaccgc
     1981 aaacatatgg aggaacgcct ccagcgtctg gcgctctacg acaccctgac ggggcttccg
     2041 aaccgtgcgc tcttcttcga ccgcgcaaac caccttctgg ccgccgccag gcggaaccgg
     2101 cacctgctcg cgctcctgta catggatctc gaccgtttca agaccgtcaa tgacgggctt
     2161 ggacatgaaa tgggtgacct cctgctcgcc gaggccgcgc ggcggatagc gtcatgcacg
     2221 cggcaggaag atacggtcgc ccggatgggc ggagatgaat tcatcggcct ctgcggaaag
     2281 atcgtttcac cggaggacgc agcggtcgtg gcgcg
//
LOCUS       DASSLD010000491         3380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_400199,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000491 DASSLD010000000
VERSION     DASSLD010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400199"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VFK23_08870"
     CDS             <1..586
                     /locus_tag="VFK23_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET7319234.1"
                     /translation="SAAGIIAVADTPREHSKEAVRELRALGLDVVMLTGDNRRTAQAV
                     ARELGITRVMSEVLPQDKMDVVKKLQSEGRIVAMVGDGINDAPALTQADVGIAIGTGT
                     DVAIEASDVTLIKDDLRVVVAAMRLSRRTMQTIKMNLFWAFFYNVIGIPVAAGVLFPF
                     LGAKGLLNPMYASAAMAFSSVSVVTNSLRLRRFE"
     gene            668..1147
                     /locus_tag="VFK23_08875"
     CDS             668..1147
                     /locus_tag="VFK23_08875"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HET7319235.1"
                     /translation="MKFIADVMLGRLAKRLRLLGYDVLYDPALDDNDIVRLSLEQRRV
                     ILTRDAALAARPLASNHLFVRSDHVEEQLEQVLTISPRDASPLTRCSLCNEPLASMTR
                     QEARDLVPPYVFEKNETFQQCTICGRVYWEGTHVWRMIGRAGGKKKRPFRPNGTDRP"
     gene            complement(1287..2228)
                     /locus_tag="VFK23_08880"
     CDS             complement(1287..2228)
                     /locus_tag="VFK23_08880"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HET7319236.1"
                     /translation="MGTLGKYLHDAREARGIDLRDAAQQTRISLQYLRALEEENFEKL
                     PGDVFVRGFLKNYARFLRLDEAEVMKQYAEMVQKPAPAGVANQVQEAAAEKHEKPRRS
                     SKIPVEPFVWGAGIVIILILFLHTALPSRHRKAPRMAAEAPTQVLSELSSTVTPSAPE
                     KLYLEVFALDNTWLLVRTDSSPQKKAVLKKGESLIWSADQSFLLSYGSAGAVRLMLNG
                     QELTVNEPKNAVVRDLTITAAGVISRKTPTEQRPERPRIKPAAAVQTKPSPTTTPQQA
                     PSVPAPQQTPSVGSPQPPQKTLPLPPAPGKMPKRLVQ"
     gene            complement(2232..2972)
                     /locus_tag="VFK23_08885"
     CDS             complement(2232..2972)
                     /locus_tag="VFK23_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7319237.1"
                     /translation="MRTLAVIIVLVLTGCVSTGSNIEKSKMAEGYSQKGLAYFQERNY
                     ELASVEFNRAIQTDSSNKLSFYYLGVISDLQGKSDEAIKYYKEAISLDGDFSEAYNAL
                     GAAYSKQQKWNDALKNYKKALENKLYTTPHVPYLNMGRVYMAQKDYPKAIEAYREAKR
                     YVSLDIIIFELGQALFEAGRTKEAIAEFKEGVGLAPQNADIRYALALAYLKDGNKKAA
                     AVEFRKAVELAPKSELAQKANDYLKILR"
     gene            complement(3071..>3380)
                     /locus_tag="VFK23_08890"
     CDS             complement(3071..>3380)
                     /locus_tag="VFK23_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319238.1"
                     /translation="AEVPEMRAAPKSQPTEKGAGTGEAAVPAPERTRPRPAAPAAAGP
                     RTASYADSSSTDRQDIRRKTSQDDIGKPLKTPMQKRAEENAGVVRRDREALARLLSSF
                     "
BASE COUNT          633 a          996 c          980 g          771 t
ORIGIN      
        1 gagcgcggcc ggcatcatcg cggtggccga cacgccccgg gaacactcga aagaggcggt
       61 gcgggaactc agagcgctcg gactcgacgt cgtcatgctc acgggagaca accgccgcac
      121 cgctcaggcc gtcgccaggg agttggggat cacccgcgtg atgtccgagg tcctccccca
      181 ggacaagatg gacgtcgtga agaagctcca gtccgaaggc cggatcgtgg ccatggtggg
      241 agacggcatc aacgatgcgc cggcgctgac ccaggccgac gtgggcatcg ccatcggcac
      301 gggcactgac gtggcaatcg aggcgtccga cgtcactctc atcaaagacg atctcagggt
      361 cgtcgtggct gccatgcggc tgtcgcgcag gaccatgcag accatcaaga tgaacctgtt
      421 ctgggccttc ttctacaacg tcatcggcat tcccgtggcc gcaggcgtgc tcttcccatt
      481 cctgggggcc aaggggcttt tgaaccccat gtacgcttcg gccgccatgg ccttcagctc
      541 cgtgagcgtg gtgacgaatt ccttgcggct gagaagattt gaatgagcag acggtagaca
      601 gtaggcagca tacaggaaat atcattgccc ccgtctgctg tccactgttt actttctaca
      661 ggatcgtatg aagttcatcg ccgatgtcat gctgggaagg cttgccaagc gcctgcggct
      721 cctgggttac gacgtccttt acgacccggc cctggacgat aacgatattg tccgcctgtc
      781 gctcgagcag cggcgcgtga ttctcacgcg ggacgcggcc ctcgccgcgc ggcctctcgc
      841 gtcgaaccat ctgttcgtca ggagcgacca cgtcgaggaa cagctcgagc aggtgcttac
      901 aatctctccc cgggacgcat ctcccctcac ccgctgctcc ctgtgcaacg aaccgctcgc
      961 atccatgacg cggcaggagg cgcgggacct cgtcccgccc tatgtgttcg aaaaaaatga
     1021 aacgtttcaa caatgcacga tctgcggtag ggtttactgg gaagggaccc atgtctggag
     1081 gatgattggg cgggcaggcg ggaaaaagaa aaggccgttc cgccccaacg ggaccgaccg
     1141 gccttgagaa ttctctgcaa ttctacgtaa agttgtaggt tattccgaag tcgatagctt
     1201 cgggctcaca ttcttcacag caacaaaacc gtaaaaggac aggcgggaag gaattcatcc
     1261 cctccgaagt tgttctctcc cggtgtttat tgaacaagcc tcttcggcat ctttcccggt
     1321 gccggaggca gcggcagggt cttctgcggc ggctggggtg agccgacaga cggagtctgc
     1381 tggggcgcgg ggacagatgg tgcctgttga ggcgtggtcg taggagacgg cttcgtctgc
     1441 accgccgctg ccggtttgat cctcggtctc tccggccgct gctcggtcgg ggtctttcgg
     1501 cttatgacac ccgctgccgt gatggtcagg tcacggacga cggcgttttt gggctcgttt
     1561 accgtcagtt cctgcccgtt gagcatcaat ctgacggccc ccgcgctgcc gtagctcagc
     1621 agaaagctct gatccgcgct ccagatgagg ctttcgcctt ttttcaggac cgcctttttt
     1681 tgaggactcg aatcggtcct gaccagaagc caggtattat cgagggcgaa tacttcgaga
     1741 tacagttttt ccggagcgct cggcgtaacc gtggaagaga gttcagaaag aacttgcgtc
     1801 ggggcctccg ccgccatgcg aggtgccttc cgatggcgcg agggcagcgc ggtatggaga
     1861 aagagaatga ggatgatgac gatgccggca ccccagacaa aaggttcaac cgggattttc
     1921 gaggaccggc gcggcttttc atgcttttcc gctgccgcct cctggacctg attcgcgacg
     1981 ccggcgggcg cgggcttctg aaccatttcg gcgtattgtt tcatgacctc ggcttcgtcg
     2041 aggcgcaaaa accttgcgta gttcttgaga aaccccctga cgaatacgtc gccgggcagc
     2101 ttttcgaaat tttcctcctc cagcgccctc aggtactgaa ggctgatgcg ggtctgctga
     2161 gcggcatcac gcaggtcaat tccgcgcgcc tcccgggcgt cgtgcagata ttttcctaaa
     2221 gtccccatcc cttacctcag gattttcaga taatcattcg ccttctgggc gagttcgctc
     2281 ttgggagcga gttcaacggc ctttctgaac tcaactgccg cagccttctt gttgccgtcc
     2341 ttcaagtatg ccagcgccag cgcatatcgt atatcggcat tctgcggagc aaggccgacg
     2401 ccctccttga actccgcgat cgcttccttc gtccggcccg cctcgaagag cgcctgaccc
     2461 agttcaaaga tgatgatgtc gaggctgacg taccgcttgg cttcgcgata ggcctctata
     2521 gccttggggt aatccttctg cgccatatat acgcgcccca tgttcaggta cggcacatgg
     2581 ggggtggtat aaagcttgtt ttccagggcc tttttataat ttttaagcgc atcgttccat
     2641 ttctgctgtt tcgaataagc cgccccgagt gcgttgtagg cctcggaaaa atcaccgtcc
     2701 agactgattg cttccttgta atattttatc gcctcgtcgg atttgccctg caaatcgctg
     2761 atcaccccga ggtagtagaa ggacaatttg ttcgagctgt cggtctggat ggcgcgatta
     2821 aattcaacag aggcgagttc gtaattcctc tcctgaaaat acgcgagtcc cttctgggaa
     2881 tagccttcgg ccattttgga tttttcgatg ttgctgccgg tcgaaacaca tccggtaaga
     2941 accagaacga tgatgactgc caaggtcctc atgaggatac ccctttgaag ctcccaaagg
     3001 atgactctcg gagcttccat tcgcaaagtg aaagtattat cttattcgct cgctacgctt
     3061 gctcccgaat tcaaaacgac gagagcaggc gcgccagcgc ctcgcggtcc ctgcgtacga
     3121 cgccggcgtt ttcctccgcc cttttttgca tcggcgtttt cagcggcttg ccgatatcat
     3181 cctggctcgt ttttcgcctg atgtcctgcc tgtctgttga ggaggagtcg gcataagaag
     3241 cggtcctggg acccgctgct gcaggggctg cgggcctcgg ccttgttctt tcgggagcag
     3301 ggactgcggc ctccccggtg cccgccccct tttcagtcgg ctgtgacttc ggcgcggccc
     3361 tcatttcggg gacctcggcg
//
LOCUS       DASSLD010000492         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1530150, whole genome shotgun sequence.
ACCESSION   DASSLD010000492 DASSLD010000000
VERSION     DASSLD010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530150"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VFK23_08895"
     CDS             complement(<1..404)
                     /locus_tag="VFK23_08895"
                     /inference="COORDINATES: protein motif:HMM:NF013898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+ dependent nucleoside transporter N-terminal
                     domain-containing protein"
                     /protein_id="HET7319239.1"
                     /translation="MLFQALLGLLVFTGIAWLASENRRGVRYTTALAGVIVQLSTAAV
                     LLYVPFFKRFFLLLNNAVLALESATKAGTSFVFGYIGGAPPPFLLQDPGANFVLAFQA
                     LPLVLIIGALSGLLFYWNVLPVVVRSFSYLLQ"
     gene            complement(472..1059)
                     /locus_tag="VFK23_08900"
     CDS             complement(472..1059)
                     /locus_tag="VFK23_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319240.1"
                     /translation="MKCNITIGILIASVLLTGPSCATRSQHTPEVRSAEDVKKDEIAG
                     ILAAADEYIAEGDFKSALDLHKTAAEKHPGDEAFERGYLATIENIKKAADTSFDQDDF
                     AQAGKTYYLLLKSVPHSNGFAAGLSFTKKNLTERLDECRSTLSQQALRQYRSGNIGNA
                     ISLWKSILTFDPGNKSVKKFIDTATIQLKNLKQNN"
     gene            complement(1049..>2217)
                     /locus_tag="VFK23_08905"
     CDS             complement(1049..>2217)
                     /locus_tag="VFK23_08905"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HET7319241.1"
                     /translation="LGLDNMVFKMQQSNPDVEFIAVIGNDRKIIAHSVIGEVGKKIDD
                     VPGSEHRKSGEGADVTEAFIPSSGSLEIVSPIIAMQKRLGSVMVRVNRSALLDAQRLI
                     RRRTLIVFAVILLFGTAGSFALSSFLTKPINELSAGVSEMKEGKADRRLQIYSRDELG
                     ILTEGFNEMMTIITDQRGKLTAFAEELEESYVSTVRVLAAAIDARDPYTHGHSARVSA
                     YAVRLGKELGLDRQTLDDLEVACLFHDIGKIKTPDSILRKRNGLGPEEYREMMRHPVD
                     GAAILGKAPSLHKYILPVRHHHERFDGRGYPDGLAGKDIPLLAAMISLTDTYDAMTSD
                     RPYRSARTDEETLEELTRCAGTQLDPDLVAAFLRVIEKNRGAEIIGDVFWRHEV"
BASE COUNT          416 a          631 c          697 g          473 t
ORIGIN      
        1 ttctggagca gataagagaa ggaccgtaca acgacgggca gaacgttcca gtaaaagagg
       61 agccccgaga gcgccccgat aatgaggaca agcggcaggg cctggaaggc caggacgaag
      121 ttcgctccgg ggtcctggag cagaaagggg gggggcgcgc cgccgatgta cccgaacacg
      181 aaggacgtac ccgccttcgt agccgactcg agcgccagca ccgcattgtt caggagcagg
      241 aagaacctct tgaagaaggg gacgtagagg aggacggccg cggtcgagag ctggacgatg
      301 acgccggcga gcgcggtggt gtatctgacg cccctgcggt tctcgctggc gagccaggcg
      361 atccccgtga atacgagcaa cccgagcaag gcttgaaaaa gcatgaagca tcctctccga
      421 ccgttgacct gcgttctttc tgcgcgatta tgccacgccg gataataaat ttcaattgtt
      481 ttgcttgagg tttttcagct ggatcgttgc ggtatcgatg aacttcttga cgctcttgtt
      541 gcccggatcg aaggtgagaa tgcttttcca gagggatatg gcattgccga tgtttcccga
      601 ccggtactgg cggagcgcct gttgtgacag cgtcgagcgg cactcgtcga gtctttccgt
      661 caggtttttt ttcgtaaatg acagcccggc ggcaaagccg ttcgaatggg ggacgctctt
      721 caggagaaga taataggtct tgcccgcctg tgcgaaatcg tcctggtcga atgacgtgtc
      781 tgcggctttc ttgatgtttt cgatggtcgc gaggtagccc ctctcgaagg cctcatcacc
      841 ggggtgtttt tcggcggcgg ttttgtgcag gtcgagggca ctcttgaagt cgccctctgc
      901 gatgtattcg tccgctgccg ccaggatccc cgcgatttca tcctttttca cgtcttcggc
      961 gctccgcacc tcgggcgtat gctgcgaccg ggtggcgcag gaagggcccg tcaggagcac
     1021 ggaagcgatg aggatgccta tggttatatt acacttcatg tcgccagaac acgtcaccga
     1081 ttatttccgc cccgcggttt ttttcgatga ccctcaaaaa agccgcgacg agatcgggat
     1141 ccagctgggt gccggcgcac cgcgtgagtt cctccagggt ctcctcgtcg gtgcgcgcgg
     1201 agcggtaggg cctgtccgag gtcatcgcat cataggtgtc ggtgaggctt atcatggcgg
     1261 ccagcagcgg gatgtccttg cccgcaaggc cgtcggggta gccccggccg tcgaaccgtt
     1321 catgatggtg tctgaccggg aggatgtact tgtgaaggga cggcgccttg ccgagaatgg
     1381 ccgcgccgtc caccggatga cgcatcatct cccggtattc ctcgggcccg agcccgttgc
     1441 gcttgcgcag gatcgagtca ggagtcttga tcttgccgat atcatggaac aggcaggcga
     1501 cctcgagatc atccagggtc tgcctgtcga ggccgagctc cttcccgagc cggacggcgt
     1561 acgcggagac ccgcgccgag tgaccgtggg tgtaggggtc cctcgcgtcg atggccgcgg
     1621 cgagcacccg gaccgtggag acgtaggatt cttccagttc ctcggcgaag gcggtcagct
     1681 tgccccgctg atcggtaatg atcgtcatca tttcgttgaa gccttccgtc agtatgccga
     1741 gttcatcgcg cgaatagatc tgcaggcgcc tgtccgcctt tccctctttc atttcggaga
     1801 cacccgcgga gagctcattg atgggcttcg tcagaaacga ggacaacgca aagctgccgg
     1861 cggtgccgaa aaggaggatc acggcgaata cgatcagggt cctccggcgg atgaggcgct
     1921 gggcatcgag cagggcggag cggtttaccc tcaccatcac cgacccgagc cgcttttgca
     1981 tcgctatgat ggggctcacg atctccaggg aaccggagga cggaatgaac gcctccgtga
     2041 catcggcccc ctctcccgat ttcctgtgct ccgatcccgg gacgtcatcg atttttttcc
     2101 ctacttcgcc gatgacgcta tgagcgatga tcttcctgtc gtttccgata acggcgatga
     2161 attccacgtc cggattggac tgctgcatct tgaagaccat gttgtcgagg cccagca
//
LOCUS       DASSLD010000493         3362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_740623,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000493 DASSLD010000000
VERSION     DASSLD010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740623"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VFK23_08910"
     CDS             <1..245
                     /locus_tag="VFK23_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome B5"
                     /protein_id="HET7319242.1"
                     /translation="LWKNGSHLSKHAAGNDLTELLATAPHGEEKVLTMPQVGKLLASG
                     ERLKRPFHLRLFYFFAYMNLALVFVITFVIALWRWW"
     gene            350..571
                     /locus_tag="VFK23_08915"
     CDS             350..571
                     /locus_tag="VFK23_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HET7319243.1"
                     /translation="MSKEYPILADLEPGTYYWCTCGKTKTPAFCDGSHKMTDSKPLAF
                     IITEKKKVALCNCKRTKTPPFCDGAHAKP"
     gene            complement(661..>3362)
                     /locus_tag="VFK23_08920"
     CDS             complement(661..>3362)
                     /locus_tag="VFK23_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET7319244.1"
                     /translation="MRGDFLLSYLLLRKLPVYYHEVGAVSRIRDLSTEIDSWGNDPVL
                     YFVRKQVHVNASNYNVRLIERVLTAWAGNDPGPLQNAVPRDVSASLAPGLLEKYSRVI
                     RPLFESLGVVDEKGLHVERLIGIPEEEFRKRLGEGRLDEISTKVVLLCRLYQEIVGKY
                     SFVGREPGRADIAASLAACLGRMRKNVGIVRTPEKTVAQESLYFKRHIAFGIPSVLGT
                     YHEPKFDALAELLRDDAEIGVLLEEVIAGIQKRERDFSPAEAAEWLHVLVAASEALSL
                     HGLGNRQVEEFVAITGSGGLRLSQAADLLRMWQKELAWMVDYFNRTFHLPVVEIIKQF
                     PREDLPDSFMNLDPSLPDFTNKAADIVIRDMLSSVPGLVEADRLIELLLRAVTSRAEG
                     GEDDLLDGSRAEGDRQYFALHALGDEDAMRLAPVLGGKAKNLIHLANRGFQVPAGVVF
                     SARLTASYQTAVDGPDFTRALRQAVGTIEERTGRVFGDGANPLFLSVRSGSYVSMPGI
                     LQTVLYCGMNRETLRGLVSRTGDPRFAWDSYRRFIEHYGAVVMGLDGSIFDDITDGAV
                     KGFGLESREALNAAQLERLVSRYEDELVKRGLAVPDDVWEQLRQSVRSVYASWYGERA
                     VQFRRATKTSAHWGTAVVVMQMVYGNRAGSGASVFFTRNPFTGEKGIYGETREGAAGE
                     DLVHGKQRNRSIAEMDPELFDLHRKLAGDVEEAMGGLPQEVEVTYDADGNGARRIWVL
                     QTRRMEVSTGAAASFDEICRMESSIIGRGLAAHGGAQSGVASFAKTPEEVLRLRRESG
                     MPVILLRTTANTDDVSLMPVIGGIVTSSGGVTSHAAVLAGNFGVDAVVACTDMTIGAD
                     ERGVPVARIGGVEVREGSFISIDGSTGLVFSGICLGLGKEEKG"
BASE COUNT          632 a         1118 c          992 g          620 t
ORIGIN      
        1 ggctctggaa gaacggttcc catctgtcca agcatgcggc aggaaacgat cttacggaac
       61 tccttgcgac cgcgccccac ggcgaggaaa aggtcctcac catgcctcag gtggggaaac
      121 tgcttgcgtc gggagaaagg ctgaaacggc ctttccacct gcgcctcttc tattttttcg
      181 cctacatgaa cctcgccctc gtctttgtga tcaccttcgt catcgccctc tggcgatggt
      241 ggtgaaatcg accgcgtccg cgatgcaagt aggggcgggc aacagaccgg aacgaagaca
      301 agcagtcttt ggtataatca cgtcaaggaa aatctacagg agggaaaaaa tgagcaaaga
      361 atatccgata ctggccgacc tcgaacccgg cacctactac tggtgcacgt gcgggaagac
      421 caagacgccg gccttctgcg atggctccca caagatgacc gattcaaagc ccctggcgtt
      481 catcatcacg gagaagaaga aggtcgccct ctgcaactgc aagcggacca agacgccgcc
      541 gttctgcgac ggcgctcatg cgaagccctg acgataaccg ggttgtccct cggacgggac
      601 aatccgcttt ttcttctcct ggggaaggtg cagtgcactt ttcccccgat gcgctcaccg
      661 ttatcccttt tcttccttgc ccagccccag gcagatacct gaaaatacca ggcccgtcga
      721 cccatcgatg ctgatgaacg atccctccct gacctccacg cccccgatcc gggcgaccgg
      781 cactccccgt tcatccgcgc cgatggtcat gtccgtgcag gcgacgacgg cgtcgacgcc
      841 gaaatttccc gccagcacgg cggcatggga ggtcacgccg cccgacgagg tgacgatgcc
      901 gccgatcacc ggcatgaggg aaacgtcatc cgtatttgcc gttgtccgga ggaggatgac
      961 gggcatgccc gattccctgc gcagtcgcag gacctcctcg ggggtcttcg cgaacgaggc
     1021 gacgccgctt tgcgcccctc catgggccgc gaggcccctg ccgatgatgc tcgattccat
     1081 gcggcatatc tcatcgaagg aggccgcggc cccggtgctc acctccatgc ggcgcgtctg
     1141 cagcacccag atccgccgtg cgccgttccc gtccgcatcg taggtgacct ccacctcctg
     1201 cggcagcccg cccatggcct cttcgacgtc cccggccagt ttccggtgca gatcgaacag
     1261 ttccgggtcc atctccgcga tgctcctgtt ccgctgtttt ccgtgcacca ggtcctcgcc
     1321 tgccgcgccc tcccgcgttt cgccgtagat tcccttttcg ccggtgaagg gattgcgcgt
     1381 gaagaacacc gaagcccccg accccgcccg gttgccgtac accatctgca tcacgaccac
     1441 cgccgtgccc cagtgcgccg aggttttcgt ggccctgcgg aactgaacgg cgcgctcgcc
     1501 gtaccacgac gcgtagaccg accggaccga ctgccggagc tgctcccaga catcgtcggg
     1561 gaccgccaac cctcgtttca caagctcgtc ttcatatcgg ctgacgagcc tttcgagttg
     1621 agcggcgttc agcgcttcgc ggctttcaag gccgaagcct ttcaccgcac cgtccgttat
     1681 gtcgtcgaaa atcgagccgt ccaggcccat gaccaccgcc ccgtaatgct cgatgaacct
     1741 gcggtacgaa tcccaggcaa aacggggatc gcccgtccgg ctgacgagcc cccgaagcgt
     1801 ttcccggttc atgccgcagt agagaacagt ctggaggatg cccggcatgg agacatagga
     1861 gccgctcctg accgaaagga agagcgggtt tgccccatcg ccgaacaccc ggcccgtcct
     1921 ctcctcgatg gtcccgaccg cctgcctgag cgcccgtgta aaatcgggtc cgtccacggc
     1981 cgtctggtac gatgcggtaa gacgcgccga aaaaacgacc ccggcgggga cctggaagcc
     2041 gcggttcgcg agatggatga ggttcttcgc cttgccgccg agcacggggg cgagtctcat
     2101 cgcgtcctcg tcgccgagtg catggagcgc aaagtattgc ctgtccccct cggccctgct
     2161 gccgtccagc aggtcgtcct cccccccttc cgcgcgggag gtaaccgcgc gaagcagcag
     2221 ttcgatgagc ctgtccgctt ctacgagtcc cggcacgctg ctcagcatgt ccctgatgac
     2281 gatgtccgcg gccttgttcg taaagtccgg caaggaaggg tcgagattca tgaaggagtc
     2341 aggcaggtcc tcccggggga attgtttgat aatttcgacg accggcaggt ggaacgtacg
     2401 gttgaaataa tccaccatcc aggcgagttc cttctgccac atcctgagca ggtcggcggc
     2461 ctgggaaagg cggaggccgc ccgatccggt gattgcgaca aactcctcca cctgcctgtt
     2521 gccgaggccg tggagcgaga gcgcctccga ggcggcgacg aggacgtgaa gccactccgc
     2581 ggcctccgca ggagaaaagt cacgctcccg cttctgtatg ccggcaatga cctcctcgag
     2641 gagaacgccg atctcggcgt cgtcccgcag gagttccgcc agggcatcga acttcggctc
     2701 gtggtaggtg ccaaggaccg aggggatgcc gaacgcgata tggcgcttga aatagaggga
     2761 ctcctgagca acggtctttt ccggggtgcg cacgattccg acattcttcc gcattcgccc
     2821 aaggcacgcc gcgagagacg cggcgatgtc cgcgcgcccc ggctccctgc cgacgaagga
     2881 gtattttccg acgatctcct ggtacagacg gcacagaagc acgaccttgg tggaaatttc
     2941 gtcgagccgt ccctccccga gccttttcct gaattcctct tccgggatgc cgatgagccg
     3001 ttcgacgtgg aggcccttct catcgacgac gccgagggat tcgaacagcg gcctgattac
     3061 ccgggaatac ttttcgagga gccccggcgc aagactggcc gagacgtccc ggggaacggc
     3121 gttctgcagc gggccggggt cgttccctgc ccaggcggtg aggacccgct cgatgagccg
     3181 gacgttatag ttgctggcat tcacgtgcac ctgcttgcgg acaaagtaga gcaccggatc
     3241 gttaccccac gagtcgatct cggtggaaag atcccggatc ctgcttacgg cgcccacctc
     3301 gtggtagtag acgggcagct tccggagaag caggtagctc agcagaaaat ctccgcgcat
     3361 gg
//
LOCUS       DASSLD010000494         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_989035,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000494 DASSLD010000000
VERSION     DASSLD010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989035"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1525)
                     /locus_tag="VFK23_08925"
     CDS             complement(<1..1525)
                     /locus_tag="VFK23_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7319245.1"
                     /translation="MIAKIIEYSARNRFIIFLLVGFLSLWGWMSLKNTPLDAIPDLSD
                     TQVIIFTEWVGRSPNLVEDQITYPISATLLAAPKVQAVRGFSYLGSSFIYVIFEEGTN
                     IYWARSRVLEYLQAVKGKIPADVNPVLGPDATSVGWGFSYAVVDETGQYDLAQLRTAQ
                     DYIAKLALESVPGVSQVASLGGFVKQYQVTVDPNRLAAYNIPIMKVMEAVKRSNSDVE
                     GRVLELSGVEYMLRGRGYIKSTRDLETIPVGAQNGTPVYLRDVASVQLGPEIRRGLMD
                     LDGKGEAVGGIVVVRFGENVLNVIERVKEKIRTDVEPSLPKGMKIVVTYDRSDLIHHS
                     INTLKEEIIKLSIAVSVVCIIFLFHIPSALVVILTLPVAIIISFILMQYLNVTSNIMS
                     LSGIAIAIGAMVDASIIMVENAHKKLEEWYCDAQKQRECSRFDVVIDAAKEVGPSLFF
                     SLLVITVGFLPVFTLQAQAGRLFKPLAYTKTFAMLFSSFLAVTLTPVLMTLFIRGKIW
                     "
     gene            complement(1525..>2615)
                     /locus_tag="VFK23_08930"
     CDS             complement(1525..>2615)
                     /locus_tag="VFK23_08930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7319246.1"
                     /translation="GGAAPAQPEPAKKPAGTQPPEQNEAEKAPVVEIPLDKQQLIGVK
                     TVAVAVHQLQKTIRTVGRIEYDERKLATINTKFEGWVEKLHVDYVGKQVRKGDPLAEI
                     YSPELFATQQEFINSLKWKGQSSEVKDESVNKMLTRDADAVLEAARQRLRLWDISEEQ
                     IKSIAESGKPIRTLTIYSPVDGYVVQKSVLQGMRIMPGEKLFDIADLSTVLIIADIYE
                     YELPLIRLGDTAKISLSSFPGKELTAVIDYIYPALTGETRTAKVRFSVPNPGGRLKPQ
                     MFTNVEIKTGMGSRLAIPEEAVIDTGRRQIVYVDKGEGYFEPREVTLGVRAGGMVEVL
                     KGLKAKEKVASAATFLIDSEARLKGIVK"
BASE COUNT          484 a          783 c          768 g          580 t
ORIGIN      
        1 tccagatctt tccccggatg aacagggtca tcaaaaccgg tgtcagggtg accgcgagga
       61 acgaggagaa cagcatggca aaggtcttgg tataggccag gggtttgaag agcctgcccg
      121 cctgggcttg gagcgtgaag accggcagga accccacggt gatcacgagg agcgagaaga
      181 aaagcgacgg tccgacttcc ttggccgcgt cgatgacgac gtcgaatcgg gagcattccc
      241 tctgcttctg cgcatcgcag taccactcct cgagcttctt gtgggcgttc tcgaccatga
      301 tgatcgaggc gtccaccatg gcgccgatgg cgatggcgat gccggaaagg ctcatgatgt
      361 tcgaggtcac gttcaagtat tgcatgagga tgaacgaaat gatgattgct acgggcagcg
      421 taagaatcac caccaaggcg ctcggtatgt ggaacaggaa tatgatgcag accacggaga
      481 ccgcgatcga gagcttgatg atctcctcct tcaaggtgtt gatggagtga tggattaggt
      541 cggagcggtc gtaggtgacg acgatcttca tacccttggg gaggctgggc tccacgtcgg
      601 tcctgatctt ttcctttacg cgctcgataa cgttcagcac gttctcgcca aagcgcacga
      661 ccacgatgcc gcctaccgcc tcgcccttgc cgtccaggtc catgagacca cggcggatct
      721 ccgggccgag ctggacgctt gccacgtccc gcaggtatac gggcgtgccg ttctgcgcgc
      781 ccaccggtat ggtttcgagg tccctggtcg acttgatata ccccctgccg cgcagcatgt
      841 actcaacgcc ggagagctcc aggaccctgc cctccacgtc gctgttgctc ctcttgaccg
      901 cctccatcac cttcataatc gggatgttgt acgccgcgag ccggttcgga tcgaccgtga
      961 cctggtactg cttcacgaac ccgccgaggc tcgccacctg ggaaacgcca gggacgcttt
     1021 caagggcgag cttggcgata taatcctggg cagtgcggag ctgggccagg tcgtactggc
     1081 cggtctcgtc aaccacggca taggagaatc cccagcccac gctcgttgcg tcgggcccga
     1141 gcacgggatt cacgtccgcg gggatcttcc ccttcaccgc ctggaggtac tcgagcacgc
     1201 ggctcctggc ccagtagata ttcgtgcctt cctcgaagat cacgtagatg aaagagctcc
     1261 ccaggtagga gaagccgcgc acggcctgca ccttcggcgc ggccaggagc gtggcgctga
     1321 tgggataggt gatctggtcc tcgacgaggt tggggctcct gcccacccat tcggtgaaga
     1381 tgatcacctg cgtgtcggag aggtccggaa tggcatcgag cggcgtgttc ttgagcgaca
     1441 tccaccccca gagactgagg aaaccgacga gaagaaagat gatgaacctg tttcgtgcgc
     1501 tgtattcgat gattttcgcg atcattactt cacgatcccc ttcagcctcg cctctgagtc
     1561 aatcaggaag gtggccgcgg aagcgacctt ttctttggct ttcaggcctt tcaggacctc
     1621 caccatcccg ccggcccgga ccccgagcgt cacctcccgg ggctcgaaat atccttcgcc
     1681 tttgtccacg tacacgatct gccggcgacc cgtgtcgatg acggcctcct cggggatcgc
     1741 aagcctgctt cccattccgg tttttatttc cacgttcgtg aacatctgcg gtttaagccg
     1801 cccgccgggg ttgggaacgg agaaccgcac cttcgcggtc cttgtttcac ccgtgagcgc
     1861 gggatagata tagtcgatga cagcggtaag ctccttgccg ggaaaggagc tcagcgagat
     1921 cttcgccgtg tccccgaggc gtatcagggg aagctcgtat tcatagatat cggcgatgat
     1981 caaaaccgtg gacagatcgg cgatgtcgaa gagcttttcc ccgggcatga tcctcatccc
     2041 ctggagcact gacttctgta ccacgtaccc gtccacgggg ctgtaaatgg tcagcgttcg
     2101 aatcggtttt ccgctttccg ctatggactt gatttgctcc tccgatatgt cccaaagccg
     2161 gagccgctgc ctggctgcct caaggacagc atccgcgtcc cgcgtgagca ttttgttgac
     2221 gctctcgtcc ttcacttcac tgctctgtcc cttccacttc aggctgttga tgaactcctg
     2281 ctgggtcgca aaaagctcgg ggctgtaaat ctcggcgagc ggatcgccct ttcttacctg
     2341 ttttccgacg taatccacgt ggagtttctc gacccagccc tcgaacttgg tattgatggt
     2401 ggcgagcttt ctctcgtcat actcgatccg tccgacggtc ctgatggtct tctggagctg
     2461 atgaacggcg acagcaaccg tctttacgcc gatcaactgc tgtttatcga gggggatttc
     2521 gacgacaggg gctttctcgg cttcgttttg ctccggcggc tgcgtgcctg ccggcttttt
     2581 agcgggttca ggctgggcgg gggccgcgcc gccgt
//
LOCUS       DASSLD010000495         4961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_954891,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000495 DASSLD010000000
VERSION     DASSLD010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954891"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VFK23_08935"
     CDS             <1..188
                     /locus_tag="VFK23_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319247.1"
                     /translation="EKYQDQIGRILIARKRVRSELAKLGEVGSERYEDVRKDVERAIN
                     DLKKGIDEIVERIRKAA"
     gene            558..1553
                     /locus_tag="VFK23_08940"
     CDS             558..1553
                     /locus_tag="VFK23_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HET7319248.1"
                     /translation="MATLTRSTIAAALAAMIATSVPTWVRADDAPVAVQIVDALNKHF
                     GVHPGFRANHAKGIVVEGSFKASPEAARLSRSPLFTGVKLPVTVRFSDAGGPDLPDAS
                     PVANPHGMAIKFHLPGGGESDIVVNAFKFFQVATPEDFRDLHLAVASSPPGSPMSAQL
                     KAFLASHPSVAKANATLGTPASFAEEQYFGVNAFVFTNKSGQKQAFRYIIAPEKVVHL
                     SKEEAAKKSPDYLMNDLPARLAKGPVMFHIKAQLAAAGDPTRDATQAWPDDRKVVDLG
                     VLTIERTVADSAAAQKKLLFLPGRLTDGIEPSDDPLIAARDAAYAVSFGRRSGNP"
     gene            complement(1829..2338)
                     /locus_tag="VFK23_08945"
     CDS             complement(1829..2338)
                     /locus_tag="VFK23_08945"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HET7319249.1"
                     /translation="MPVGLWPYNIGVTPNGKLALTADQGNAGRSDGHVDTVSVIDLEA
                     NPPRVIDHVVVGDAPEGLEISPTGKIAVAMLLNGNDGPHNAWFYHKNGRVAVLKIDGK
                     KVTKVGEVEVGGVPEGAVFSPDGKYLYVGNFNDQDVSILKVDGTKVTDTGKRLQLPGH
                     PASMRGRVR"
     gene            2895..3137
                     /locus_tag="VFK23_08950"
     CDS             2895..3137
                     /locus_tag="VFK23_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7319250.1"
                     /translation="MPEGHSENPVGFSFCGTCGQPLGEAGHPGIISEPESERRHVTVL
                     FTDLTGYMAMCERLDPEDIKEVMSRIFGEIAQVVTR"
     gene            3465..4727
                     /locus_tag="VFK23_08955"
     CDS             3465..4727
                     /locus_tag="VFK23_08955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7319251.1"
                     /translation="MYDFMLTEEAKTTVREVRDFIRNAVSPEYIRAMDCDEIRFPREF
                     VENLGQRNLLGLRFARNWGGRELPWTAEVAAIEEVGVLGTALGCAYVMPSIVGEALDK
                     FGTDSQKAKYLKPLLAGKLIAAEALTEPRGGSDFFGATTRAVKDGDEYVITGQKRFVV
                     GAEGADFFIVYCRTNPDPNAHPHNRISLFIVERGPGVDVEYRYGLMGTRGGGTGRLVF
                     REARVPAENILGGINMGALIFNQMMIPERLTSAAGALGVRGSLEVAARYSDKRTAFGQ
                     KIRKYQAISFMIGDGITKLDAATALVYLAARAVDAGAPNMRRLVSEAKKFATDAAWEV
                     ANIAMQVMGGIGYTDVYPIEKAVRDFRLTQIWTGTNEIMNLLVQHEYYGEVLADTSDR
                     RNIEDDAMHANDTEKVYVDEDQEKGTRR"
BASE COUNT         1111 a         1343 c         1493 g         1014 t
ORIGIN      
        1 aggaaaaata ccaggaccag atcggaagga ttctgatcgc gaggaaacgg gtccgttcgg
       61 aactggcgaa attgggggaa gtgggaagcg agaggtacga agatgtccgg aaagacgtgg
      121 agcgtgccat caacgacctg aagaagggga tcgatgagat cgtcgaaaga atccggaagg
      181 cggcctgaaa gatcatcggt tcgattcgcc gctccagggg tgtatgcggc gcctcgggcg
      241 gtccatccga ggcgccgcct tctcccgata ttctccccga acgccgtacc gaatcacggc
      301 gggggggtgg gttcaccagc ttcgattcgt tccatcgagg cgtagtgatt gacctttccc
      361 ccggaattag gccaactggg caaacaactg ccacttgaag aggaagattg agtaccctgt
      421 tctaagatac gggtggaccg atcacggggg gctggtcagt cttgaccttg attgaaattc
      481 tgggggaatg ttgcatatag aatagacagt acaaacagtg acgggtcgga attacccaga
      541 caaaggagga ataccctatg gctaccttga ccaggagcac cattgcggcc gcattggccg
      601 caatgatcgc gacgagcgtt ccgacctggg tgcgcgcgga cgatgcgccc gtggccgtcc
      661 agatcgtcga tgcgctgaac aagcactttg gcgtgcatcc tggctttcgg gcgaatcacg
      721 cgaagggcat cgttgtcgaa ggcagtttca aggcctcgcc tgaagccgcg agactgagca
      781 ggtcgccgtt gttcaccggc gtgaagctcc cggtaaccgt gcgcttttcc gatgcgggcg
      841 gacccgatct ccccgatgcc tcgccggtgg caaacccgca cggtatggcg atcaagtttc
      901 atctgcccgg cggcggtgaa agcgatatcg tcgtcaacgc gttcaagttc ttccaggtgg
      961 cgactcccga ggatttccgc gacctgcatt tggcagttgc ctcaagtccc cccggttcgc
     1021 cgatgtcagc ccagctcaag gcttttcttg ccagccaccc gagcgtcgcg aaagcaaatg
     1081 caacgctcgg tacgccagcc agtttcgccg aggagcagta tttcggcgtc aacgcgttcg
     1141 tgttcaccaa caagtccggc caaaaacagg ccttccgcta catcatcgcg ccggaaaagg
     1201 tcgtgcacct gagcaaggag gaagcagcga aaaaatcgcc cgactatctg atgaacgatc
     1261 tgcctgcgcg ccttgccaag gggccggtga tgttccatat caaggcacag cttgccgcag
     1321 ccggcgaccc cacgagggac gcgacccagg cttggcccga tgatcgcaag gtcgtcgatc
     1381 tcggcgtgct gacgatagaa aggactgtcg ccgacagcgc cgctgcgcag aagaaactgc
     1441 tgttcctgcc cggtcgtctg accgatggca tcgagccatc cgacgacccg ctgatcgctg
     1501 cgcgcgacgc cgcctatgcc gtgtcgttcg gacggcgtag cggaaacccg taacctggcc
     1561 gacaaaggcc atttaccttc tgtgaccttt tctacggaca aaggcccgat cccgggagga
     1621 aaggtcacag tattctgagg aaagaacgtc ccgatttgag atcttgaggg ttcaccagta
     1681 ccggtttcac tttcatcggg ggccccaggg gcagcagagc ggcaagtcca ctcgaatccg
     1741 ggatcttcat tcctgggtgc gagcctggga ctcgagatga acgcccactc caggtctctc
     1801 tgccccagca ccgccacaca gaagtagctt agcgaacccg cccgcgcatg gacgccgggt
     1861 ggccaggcag ttgaaggcgc ttgccggtat ccgtcacctt cgtgccgtcc accttcagga
     1921 tggatacgtc ctggtcattg aagttgccga cgtaaaggta cttcccgtcc gggctgaaga
     1981 cggccccttc gggaactcct cccacctcaa cctctccgac ttttgtcact ttcttgccat
     2041 cgatcttcag gaccgccacg cgcccgttct tgtgatagaa ccacgcattg tgcggcccgt
     2101 cgttgccgtt caacagcatg gccacggcga tcttacccgt gggactgatc tcgaggccct
     2161 ccggggcgtc gcccacaacc acgtgatcga tgacccgcgg agggttggcc tctagatcga
     2221 tgacgctcac ggtatccacg tgtccatccg agcggcctgc attgccttgg tctgccgtaa
     2281 gcgcaagctt cccgttcggc gtcacgccga tattgtacgg ccaaagccct accggcatgt
     2341 cgtacttgcc gtaggtgacc ttctgccttg cgagttagat gagttggagg tgctggagtt
     2401 cccctcagga gcaaatggaa gtcatggtgg tgcaggcgga gaacctggca cgcgttccca
     2461 tacgatgggc actacttgag aggaagccgc acggactgca aagacaacgt aatcattctt
     2521 tcgacacgga ttttgccgat gcctataagg atatcggtga tgtttgctcc atctcagctt
     2581 aatgaaatta atgacaggat ttccgtcctt ccactcggaa gatgtcgggg cggaacatct
     2641 caattgattc ggtttcggtt attgaggaaa tggccaattt gtggctccca gtgtggacgg
     2701 tccttgatat gagtagccca cgattgacag gtcaccgttg aaaatataaa attgttttac
     2761 tgcttcgatt tgtaatctct cgagacaatc gcagacccgt cccggtaagc agggaggcgt
     2821 gatgcgggct ctcgacccct cccaccaact gaagaaacaa tctcccggca ctgcgcggga
     2881 ggaattccga tgaaatgccc gaaggccact ccgaaaatcc cgtaggtttt agtttctgcg
     2941 gtacgtgcgg ccagccgctg ggtgaagcgg gccacccggg gatcatttcc gaaccggaaa
     3001 gcgagcgccg gcatgtaacc gtcctgttca ccgatctgac ggggtatatg gccatgtgcg
     3061 agcggctcga cccggaagac atcaaggagg tcatgagccg gatcttcggg gaaatcgccc
     3121 aagtcgtcac gaggtaagag gggttcatca agaagttcgt cggcgataga acctgataag
     3181 aagagtgtcc atggatgatc ctggattgca ctggcggaag gtttcccgtc aagtctgggc
     3241 tccaattccg ctctggctca cggcgctggc aatcgtcgcc attgccttat ttacctggcg
     3301 cgcatacaaa agcgaaacaa agtacggatg ggtttgataa aacgtcgctg agaaaccaag
     3361 ccacctaata actctctgga gcggacccgg ccagcgcgtg gactgaaacc agctcagcct
     3421 gagcgttagc ctgtctttgc aggcagagga aggaggctgt agatatgtac gatttcatgc
     3481 tcacagaaga agccaagact acagtccggg aagtcaggga tttcatacgt aatgcggtat
     3541 cgccggaata tatccgagcg atggactgcg atgagatccg tttccctcgg gaattcgtgg
     3601 aaaatttagg acaacggaac ctccttggcc tgcgattcgc gaggaattgg ggaggccggg
     3661 aattgccttg gacagcggag gtagctgcta tcgaggaagt cggtgtgttg ggtactgcgt
     3721 tgggttgcgc atatgtgatg ccttcgatcg taggagaagc gctggacaag ttcggcacgg
     3781 attcacagaa ggcgaagtat ctcaagccgc tgcttgccgg caagttgatc gccgctgagg
     3841 ctctgacaga accaaggggc ggatcggatt ttttcggcgc caccactcgt gccgtcaaag
     3901 atggagacga gtacgtcatc actggtcaga agagattcgt ggtcggcgcg gaaggtgcgg
     3961 acttcttcat cgtgtactgt aggaccaatc cggatccgaa tgcccatccc cacaatcgca
     4021 taagcttgtt catcgttgag agaggaccgg gcgtcgacgt ggaatacaga tacgggctga
     4081 tgggaacccg cgggggaggg accggtcggt tggtcttccg ggaggcgaga gtgcctgcgg
     4141 aaaacatcct gggcggcatc aacatgggcg ccttgatctt caaccaaatg atgataccgg
     4201 aaaggctgac atccgccgcc ggcgctcttg gggtacgggg ttctcttgag gtggcagccc
     4261 gctatagcga caagcgcacg gccttcgggc agaagatccg aaaataccaa gcgatcagtt
     4321 tcatgatcgg ggacggaatc acgaaactgg atgccgcaac ggcccttgtc tatttggcgg
     4381 cgcgagccgt ggacgcgggg gcacctaaca tgcgcaggct tgtaagcgag gcgaagaaat
     4441 ttgcgacgga cgctgcttgg gaggtagcaa acatcgccat gcaggtaatg ggtgggatcg
     4501 ggtatacaga tgtctaccca attgaaaagg cagtccgtga cttccgtctt acgcagatct
     4561 ggacaggaac caacgagatc atgaatctgc tcgtgcagca tgagtattat ggggaagtgc
     4621 tggccgacac ttcggatcgc agaaatatcg aggacgatgc catgcacgcc aacgacacgg
     4681 aaaaagtcta tgttgatgaa gatcaggaga agggaacgag acgatagttc gtaatgggat
     4741 accggccgtt cttgtatgga ttgctgctca acaccatttg tggtgtccgg aaggtcgagt
     4801 tggcacataa tctctgaact agaaatggag caggctacct gccggctggc ggccagagat
     4861 cggctcccca cgatggtcgc accgtcaagg gtcgacgtcg ggggcctcat ttcgtatgga
     4921 acgtgccaag tcgagtcgat ccgccgcatg tcgacatacg t
//
LOCUS       DASSLD010000496         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_791284,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000496 DASSLD010000000
VERSION     DASSLD010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791284"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..912
                     /locus_tag="VFK23_08960"
     CDS             70..912
                     /locus_tag="VFK23_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET7319252.1"
                     /translation="MEYKRSEIGTGIFLLTAFVIFAVMIFAVSDMKSFFKKKKDIKVL
                     FQYSDGIEKNAQVRLSGIRIGDVADVRVAPEYGDKVELTLSIYSDTVIREDTKAAIKT
                     LGLVGGKYVELAGGSSSARVLKPGETLIGEESFKLEDLTKAGLEVVGKLTNIADNLDR
                     LLGDPALSKSIRIAIQNVTEATENVKVMTSGKEEVAEGLKNLPEMLKKLDATAANLKS
                     ITDKADVLMGKTDAVVGKTDALLGENRKNIDAILESVKDTAQNLKELTDDVKKHPWKL
                     IRKP"
     gene            954..2201
                     /gene="hisS"
                     /locus_tag="VFK23_08965"
     CDS             954..2201
                     /gene="hisS"
                     /locus_tag="VFK23_08965"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET7319253.1"
                     /translation="MSIQAIKGVKDILPADIPAWQHIEATARRLFEDYGFSEIRVPVF
                     EYTELFARSIGASTDIVEKEMYTFEDRDGKKITLRPEGTAGVVRAFVEHKLHAESQLV
                     KLYYLGPMFRHERPQKGRYRQFYQIGVEALGTDHPHVDIEVLTMLHALFARLGITGLT
                     LQVNSLGDGACRGTYREALKTYLSGKLPALCADCKRRYETNPLRVLDCKVDADKFADS
                     PVMLDHLCGPCKEHFDTVEKGLEKLGIPFEVNPRLVRGLDYYTRTTFELVMGHMGAQN
                     TVAAGGRYDGLVEEIGGPPTPGIGFALGVERAISLLDTKELRAPRPALFIAALGPEAA
                     SFALPLIHGLKSAGVRAETDYAGGSLKSQMKKADRSGARYTLIIGEQEMKEGKAVLRN
                     MQTKEQVPVSLAGILEELKAKLS"
BASE COUNT          576 a          667 c          731 g          477 t
ORIGIN      
        1 tcatttttta tcgttcccaa cgttcctgcc ttcccaccat cgtaccttca agattttctg
       61 gggtgaaaaa tggaatacaa acggagtgaa ataggaacgg gtatatttct cctgaccgcg
      121 ttcgtcattt tcgccgtaat gatattcgcc gtgagcgaca tgaagagctt ttttaaaaag
      181 aaaaaggata tcaaggtcct gttccagtac agcgacggaa tagaaaagaa cgcccaggtc
      241 cgcctctcgg ggatcaggat cggagacgtg gccgacgttc gcgttgcgcc ggaatacggc
      301 gacaaggtcg aactcacact cagcatttac agcgataccg tcatccggga agacaccaag
      361 gccgccatca agacgctggg cctggtcggt ggtaagtacg tggagttggc cgggggttcg
      421 tcgtctgccc gcgtcctcaa acccggggaa acgctgatcg gcgaggaatc gttcaaactg
      481 gaagacttga ccaaggcggg attggaggtc gtcgggaagc tcacgaacat cgctgacaat
      541 ctggaccgcc tgctgggcga cccggccctg tcgaaatcta ttcgtatcgc tatccaaaat
      601 gtcacggaag cgacggagaa cgtaaaggtt atgacttcgg ggaaggagga agtcgccgag
      661 gggcttaaaa acctgcctga aatgctgaaa aaactggacg cgaccgccgc gaacctcaag
      721 tccatcaccg ataaagccga tgtgctcatg ggcaagacgg atgcggtcgt cggcaagacc
      781 gacgctctcc tgggggagaa cagaaaaaac atcgacgcaa tactggaaag cgtgaaggat
      841 acggcgcaga acctgaaaga gctgacggat gacgtgaaga aacacccgtg gaagctgatc
      901 aggaaaccgt gatcaccggg aaggtgggaa ggtaggaggg taggaagaca cgaatgtcaa
      961 ttcaggcgat caagggcgtg aaggacatct tgccggcgga catcccggca tggcagcata
     1021 tcgaggcgac ggcccgcagg ctgttcgagg attacggctt ttcggagatc cgcgtgccgg
     1081 ttttcgaata taccgagctt ttcgccagga gcatcggggc ttcgacggac atcgtcgaaa
     1141 aagagatgta taccttcgag gaccgcgacg ggaaaaagat cacgctccgg ccggagggga
     1201 ccgccggggt cgtgcgcgcc ttcgtcgagc ataaattgca tgccgaaagc caactcgtca
     1261 agctctatta cctggggccc atgttcaggc atgagcggcc gcagaagggg aggtaccgcc
     1321 agttctacca gatcggcgtc gaggccctcg ggaccgatca tccccacgtc gatatcgagg
     1381 tgctgacgat gctccatgcg ctttttgcgc ggctcggtat cacggggctc accctccagg
     1441 tcaattccct gggggacggc gcatgcaggg gcacataccg cgaggccctc aaaacgtacc
     1501 tctcgggaaa gctgccggcc ttgtgtgccg actgtaaacg gcggtatgag accaaccccc
     1561 ttcgcgttct ggactgcaag gtggacgcgg acaagttcgc cgactcaccc gtcatgcttg
     1621 accacctctg tggcccttgc aaggagcatt tcgacacggt ggaaaagggg ctcgaaaaac
     1681 tgggcatacc cttcgaggtc aatccccggc tggtgcgcgg cctcgattac tatacacgca
     1741 ccaccttcga gctcgtgatg gggcatatgg gcgcgcagaa taccgtggct gccggcggca
     1801 ggtacgacgg cctcgtggag gagatcggcg ggccgcctac gcccggcatc ggttttgccc
     1861 tcggcgtcga acgggcaata tcgcttctgg atacaaagga actgcgggcg ccgcggcctg
     1921 ccctgtttat cgccgcgctc ggacccgagg ccgcttcttt tgctcttccc ctcatacacg
     1981 gcctgaagtc cgcgggcgtc cgcgccgaaa cggattacgc gggcggcagc ctcaagagcc
     2041 agatgaagaa ggcggacagg tcgggcgcgc gctatacgct gatcatcggc gagcaggaga
     2101 tgaaggaagg caaggcagtc ctccgcaaca tgcagaccaa ggagcaggtt cccgtctcgc
     2161 tggccggcat cctcgaagaa ttgaaggcga aattgtcata gtactacatc gaaatattgt
     2221 cattcctgcg aaagcagaat ccattttcag aaagaagtcc gtaaaagccg gtggataccc
     2281 gctttcgcgg gtatgacgcg aggcgcgacg acgctgttta tcgtatagca tgcatttttc
     2341 tgaattcatt gagcattggg agttttcatg atagtcggcg tactctccga tacccacata
     2401 ccttccgctg tacagtcgct tcctcctgtc atcttcgaca ttttcaagga t
//
LOCUS       DASSLD010000497         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1079737, whole genome shotgun sequence.
ACCESSION   DASSLD010000497 DASSLD010000000
VERSION     DASSLD010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079737"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VFK23_08970"
     CDS             complement(<1..338)
                     /locus_tag="VFK23_08970"
                     /inference="COORDINATES: protein motif:HMM:NF036808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit methyltransferase I"
                     /protein_id="HET7319254.1"
                     /translation="MKSILLKPKEERRVLRGHPWVFSNELQQMPEGIVPGDIVDVLDA
                     PGRFIGRGYINPRSLIAVRILTRKKEDIGVDFLRRRISAARALRASLGFGESYRALYS
                     EGDGLPGLIV"
     gene            complement(428..1366)
                     /locus_tag="VFK23_08975"
     CDS             complement(428..1366)
                     /locus_tag="VFK23_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET7319255.1"
                     /translation="MRNTILTVFAATGIFLSIAGSGLAMGPVVIDRVVAVVNSEIITL
                     SDLQREETLRKHDAGHDDRQMLEDMIDRKLQMAAAKRSGMDVTDKELADAVADIMKRN
                     SFSEVKQFEAALAKEGLTLEQYKIELREQMTLSRTFNKFVRSGIAVDETEVRAYYERN
                     TKTYSLPEEIRVRQIFFPLPEDATPAQAEAVKAKASEVCEQAKKGEDFMRLVRRNSPV
                     TDSQDGDLGFMQRDHMLPEIEAAARTLKPGEIAGPIQCAGGYHIIRLEEVRTPVRPYE
                     KVKDEIMNMLFQEKLENTYRSWLQTLRSDANIENRL"
     gene            1457..>2258
                     /locus_tag="VFK23_08980"
     CDS             1457..>2258
                     /locus_tag="VFK23_08980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET7319256.1"
                     /translation="MKKFLTVFLVLLAASSSALAGQSPQDKITVAAVGDIMLGGRAEP
                     FLIEFGPGYPFTEVMPFLGSADIVAGNLESAISTRGKAVENKKFTLRTGQIAARALRS
                     AGIRVVTMANNHSMDFGPLALRDTLSSLEENGVLYTGAGMDLDDARTPALVKIKGRTL
                     AFLAYSLTFPLDFFASPGRPGTAPGFADFVKADIEKVRPRADLVIVSFHWSSELLTAA
                     KDYQIELGRKAVDWGADLVLGHHPHVLQELELYKGRLIAYSLGNFVFGS"
BASE COUNT          405 a          692 c          655 g          506 t
ORIGIN      
        1 tcgacgatca agccgggcag gccgtcccct tcgctgtaga gagcgcgata ggattctcca
       61 aaaccgaggg acgcccggag ggcccgcgct gcggagatcc tccgtcgcag gaaatcaacg
      121 ccgatgtcct ctttcttccg ggtcaggatg cgcacggcga tgagggagcg cgggttgatg
      181 tagccccgac cgatgaacct gcccggggcg tccagcacgt ctacgatatc gccgggaacg
      241 ataccttccg gcatttgctg gagctcgttg ctaaagaccc agggatggcc gcgaaggacg
      301 cgcctctcct ctttcggttt gagaaggatg gatttcatgg ggggaagagc aatgcggcag
      361 atcgcatggc gcatggcgca aagcgtttaa gacttttacg ctatgcgctt tgctctatgc
      421 gcctttctca caatctgttc tcgatgttcg cgtcgctccg cagggtctgg agccagctcc
      481 ggtaggtatt ttccagcttc tcctgaaaga gcatgttcat gatttcgtct ttcacctttt
      541 catagggcct gaccggtgta cgcacctctt ccagccggat gatgtggtag ccgcctgcgc
      601 actggatcgg cccggctatc tcaccgggtt tcagcgtccg ggcagccgct tcgatctcgg
      661 gaagcatgtg atcccgctgc atgaacccga ggtccccgtc ctgcgagtcc gtcacagggg
      721 aattgcggcg gaccaggcgc atgaaatcct ctcccttttt cgcctgttca cagacctcgg
      781 aggccttcgc cttcactgct tcggcctggg ccggggtcgc atcttccggg agggggaaaa
      841 atatctggcg cacacggatc tcttcgggga gggaataggt cttcgtgttg cgctcatagt
      901 aggcgcggac ctctgtctcg tccacggcga tgccggaacg cacgaacttg ttgaatgtgc
      961 gcgaaagagt catctgctcg cggagttcga ttttatactg ttcgagggtg agtccttcct
     1021 tggccagggc cgcctcgaac tgcttgacct cgctgaagga gttccgtttc atgatgtcgg
     1081 caacggcgtc ggcgagctcc ttgtccgtca cgtccatgcc ggagcgtttt gccgcagcca
     1141 tctgaagctt ccggtcgatc atgtcctcga gcatctggcg gtcatcgtgc ccggcgtcat
     1201 gcttccggag cgtctcctcg cgctggaggt cggagagcgt aatgatctcg ctgttcacca
     1261 cggcgacgac gcggtcgatg accacgggcc ccatggcaag gccactgccg gcgatggaaa
     1321 ggaatatgcc ggtcgctgca aatacggtca gtatcgtgtt cctcatggaa aagctccctc
     1381 tcgcggactg cctgcttttt ctgttgcctt ttgacggccc ctctattatt atttcatgta
     1441 cacggcattc agcattatga aaaaattctt aacagtcttc ctggttctcc tcgcagcgtc
     1501 atcatcggct ttagccggcc aatcgcccca ggacaaaata accgtcgccg ccgtcggtga
     1561 tatcatgctc ggcggtaggg cggaaccatt cctcatcgag ttcggccccg gctacccctt
     1621 caccgaagtc atgccgttcc tgggaagtgc cgacatcgtg gcgggaaacc tcgagtccgc
     1681 catatccacc cgcggcaagg ccgtggaaaa caagaaattc accctgcgga ccggacagat
     1741 agccgcgcgg gcgcttcgga gcgcgggcat ccgcgtcgtg acgatggcca acaatcactc
     1801 catggatttc ggccctcttg cgctccggga cacgctctcg tccctcgaag aaaacggcgt
     1861 tctttacacc ggggctggca tggacctcga cgacgcccgc acgccggccc ttgtgaagat
     1921 caagggcagg acgcttgcct tccttgccta ttcactcacc ttcccgctcg atttctttgc
     1981 ctcccccggc aggcccggca cggcgccggg gtttgcggac ttcgttaaag ccgacattga
     2041 aaaagtcagg ccccgtgcgg accttgtgat cgtatccttc cactggagtt ccgagctcct
     2101 caccgcggcg aaagactacc agatagagct cggccgcaag gctgtcgact ggggcgccga
     2161 tctcgtgctc ggccatcatc ctcatgtact gcaggagctt gagttgtaca agggccgcct
     2221 gatcgcttac agtctcggaa acttcgtgtt cgggtcgg
//
LOCUS       DASSLD010000498         5068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_860918,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000498 DASSLD010000000
VERSION     DASSLD010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860918"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..998)
                     /locus_tag="VFK23_08985"
     CDS             complement(48..998)
                     /locus_tag="VFK23_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HET7319257.1"
                     /translation="MSQAQQKIATRDAYGDALVSLGKKRDDVVVLDADLSGSTKTSKF
                     AKAFPDRFFNIGIAEQDMMGTAAGLAIAGKLPFVSTFAVFATGRAWEQVRQSICYPNL
                     NVKIVASHAGITVGEDGGSHQSVEDIAVMRVIPHMTVIVPADGPETLQAIEAAAGHKG
                     PCYVRVGRNKVPTLFGEDYRFAIGRAHVFNEGRDAAIIAAGLMVAEAIKARDLLKAEG
                     IDAGVINMSTIKPIDAEAVITAAKKCGAIVTAEEHSIIGGLGGAVAEVLAESAPTPMV
                     RVGIRDAFGTSGDQEGLLRHYGISAEHIAAAVKEVIKKKR"
     gene            complement(1114..1953)
                     /locus_tag="VFK23_08990"
     CDS             complement(1114..1953)
                     /locus_tag="VFK23_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7319258.1"
                     /translation="MAVTSDQLKTLKDKARRIRIDILKMLTDCGSGHTGGSLSSADIA
                     TALYFYKMKYDPKKPGWKGRDHFILSKGHAAPLLYTVLAHAGFIEHAELCTLRRLGSR
                     LQGHPDSKYLPGVEISTGSLGQGLSVANGIALAHKLDRTPNRVYALLGDGELQEGQVW
                     EAAMTAAHYKLDNLCALVDNNGLQIDGPVAKVMGVEPIADKWRAFGWEVFDVDGHDME
                     KLVAALDKAETVKGKPAMIVCRTVKGKGSKVFEGKVEFHGTTPSKEEYEIALKELEEA
                     VCK"
     gene            complement(2100..2576)
                     /locus_tag="VFK23_08995"
     CDS             complement(2100..2576)
                     /locus_tag="VFK23_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319259.1"
                     /translation="MLTGYNTDFKFQGKIYHVQTEDGGINSPAITSLLYHQGAILASR
                     KTSYADILKADCLEDVVRELMMAQHKQMIRDLMHGKLDVNAAPHEAAYQTLAPRTSAG
                     KPAAAPKAAPVREKVPEKPAAAATPQSPKKEEKGKNLDDIILDFLAQEQKERNEQS"
     gene            2797..2883
                     /locus_tag="VFK23_09000"
     tRNA            2797..2883
                     /locus_tag="VFK23_09000"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2831..2833,aa:Leu,seq:caa)
     gene            complement(3003..3902)
                     /locus_tag="VFK23_09005"
     CDS             complement(3003..3902)
                     /locus_tag="VFK23_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7319260.1"
                     /translation="MKQMRLMAIVMVMSIITSCVSPKAPPPLTEPPKPRIALVLGGGA
                     ARGFAHVGVIRALEQEKIPIDMIIGTSVGSLIGAIYANDQNCFELEWTAFALEKDTLF
                     DYGILNAITGMGMAKGEKLEDFVRTKITTKNIENLKLPFAAVATDLNRGTRVVLDSGP
                     VAKAVHASCAIPGVFEPVNHQNRLLVDGGVVDNIPIAVAREKGADIVIAVDISENVQN
                     YNITNVVDVMVQAVNIMFDENVKYRKKEADVLITPAVGNVAMLDFSQKKRCMQAGIEA
                     AQKAVPEINIKIGEWVKKQQSPK"
     gene            complement(3951..4271)
                     /locus_tag="VFK23_09010"
     CDS             complement(3951..4271)
                     /locus_tag="VFK23_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319261.1"
                     /translation="MKKLNCWEFTHCGRQPGGDKAGELGICPVTSDEELSGVYGGIGA
                     GRACWAVVGSFCGGKIQGTYAEKLKDCTKCAFMALVKKEEELTPLGFSTKRNEIENSH
                     KKKK"
     gene            4506..4979
                     /locus_tag="VFK23_09015"
     CDS             4506..4979
                     /locus_tag="VFK23_09015"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HET7319262.1"
                     /translation="MTLCREIKHTLSGAKQTYACELLEYEPGIGMLRYVVDREYVING
                     RRLLPGDVTHALYWEDRPYTLYAWDLGGRGGRLFYFNIADSITLGREEFVWRDLSIDV
                     LIDAAGGIDILDKHELPPDVAPELRRYIDEAVALVVRRHGEIIREASERIGRHGR"
BASE COUNT          907 a         1566 c         1434 g         1161 t
ORIGIN      
        1 ggtagtacgg ttgtcatttg agaattggca tttccctgtc ccccgcatca gcgcttcttc
       61 tttattactt ccttcaccgc ggccgcgatg tgctccgccg agatgccgta gtgcctgaga
      121 agtccctcct ggtcgccgga ggtgccgaag gcatccctga tcccgacacg caccatcggc
      181 gtcggcgccg actcggcaag cacctcggca acggcgcccc ccagtccgcc tatgatcgaa
      241 tgctcctcgg ccgtgacgat cgcgccgcac ttctttgcgg cggtgatcac ggcctccgcg
      301 tcgatgggct tgatcgtgga catgttgatc acgccggcgt cgatcccctc cgccttgagc
      361 aggtcccgcg ccttgatcgc ctcggccacc atgaggccgg cggcaatgat cgccgcgtcc
      421 ctgccctcgt tgaacacatg ggccctgccg attgcgaacc ggtagtcctc tccgaagagc
      481 gtgggcacct tgttcctacc cacccgcacg tagcaggggc ccttgtgccc ggccgccgcc
      541 tcgatggcct gcagggtctc ggggccgtcc gccggaacga tcacggtcat gtgcggaatc
      601 accctcatca cggcgatgtc ctcgaccgat tggtgcgagc cgccgtcctc gcccacggtt
      661 atgccggcgt ggctcgccac gatcttgacg ttcaggttcg ggtagcagat cgactgccgc
      721 acctgctccc aggcccttcc cgtggcaaag accgcgaagg tgctcacgaa gggcagcttt
      781 cccgcgatcg cgagccctgc cgcggtgccc atcatatcct gttcggcgat gccgatattg
      841 aaaaagcggt ccgggaaggc cttggcgaat ttagaggttt tcgtcgaacc cgaaaggtcc
      901 gcgtccagca cgaccacgtc gtcccttttt ttcccgaggc tgaccagcgc gtccccgtat
      961 gcgtctctcg tagcaatttt ctgctgtgcc tgactcacgt ttccccccgt tttagacaga
     1021 cggggagacg gggtgacagg gagaggggga gaaagttcga ttcaaaaacc ggactcttcc
     1081 ccgcgtcccc gcgtctccgc gtccccgtgt cgattattta cacactgcct cttccagttc
     1141 cttcagcgcg atctcgtatt cttccttcga cggcgtcgtg ccgtggaact cgaccttgcc
     1201 ttcgaagacc tttgagccct tgcccttgac ggtgcggcag acgatcatgg ccggcttccc
     1261 ttttaccgtc tcggccttgt cgagggccgc gacgagcttc tccatgtcat ggccgtcgac
     1321 atcgaagact tcccatccga acgcgcgcca cttgtcggcg atgggctcca ctcccatcac
     1381 cttcgccacc ggcccgtcga tctggagccc gttgttgtcc acgagcgcgc agaggttgtc
     1441 gagtttgtag tgggccgccg tcatggccgc ttcccagacc tggccttcct ggagctcgcc
     1501 gtctcccagg agcgcgtaca cgcggttcgg tgtcctgtcg agcttgtgcg ccagcgcgat
     1561 cccgttcgcc acggacagcc cctggccgag ggaccccgtg gagatctcga cgcccggcag
     1621 atacttgctg tccgggtgcc cctggaggcg ggaaccgagc ctgcggagcg tgcagagttc
     1681 cgcgtgttcg atgaacccgg cgtgggccag cacggtatac agaagcggcg cggcgtggcc
     1741 cttcgagagg atgaaatggt cccggccctt ccagccgggc ttcttcggat cgtacttcat
     1801 cttgtagaag tacagggccg tcgcgatatc ggcggacgaa aggctgccgc ccgtgtggcc
     1861 ggatccgcaa tcggtcagca tcttcaggat gtcgatccgg attctcctcg ccttgtcttt
     1921 caacgtcttc aactgatcac tggtaacagc catgcacgaa acctccctga atcttattat
     1981 ggcaaccgcg aatacacgct gataagcgcg aagggaaaag acagccaccg tgttttgctt
     2041 tcgattcgcg ataattcgcg tcgcttagaa gcgcctactt ggtcagtcgc ggttagaaac
     2101 tagctctgct cgtttcgctc tttctgttcc tgggcgagaa agtcgaggat gatgtcgtcg
     2161 aggttttttc ccttctcttc tttcttcggg gattgaggag ttgctgcggc cgccggcttc
     2221 tccggtacct tctccctgac cggcgcggcc tttggtgcgg ccgcgggttt tcctgctgag
     2281 gtccgggggg caagcgtctg atacgccgcc tcatgcgggg ctgcattgac atcgagcttg
     2341 ccgtgcatca ggtcgcgtat catctgcttg tgctgcgcca tcatgagctc gcgaacgaca
     2401 tcctcgaggc agtcggcttt caggatgtcg gcgtaacttg tcttgcgcga cgcgaggatc
     2461 gccccctgat ggtagagcag agaggttatg gccgggctat tgatgccgcc gtcttcggtc
     2521 tggacgtgat agatctttcc ctggaacttg aagtcggtgt tgtaacccgt caacatagat
     2581 gtgcattata caaatcaggg gaggaagatg caagcctttt caggccgtag aaaaactttc
     2641 ccgcctgctg ttgcaggaag gctgcgaata cggaacacct tgataatcaa taaggtagcc
     2701 tcgcccttga aagcgcacta ttgaccgatt cgccgctttt tcaaagcccc gttttcactt
     2761 gacaggacat tgggcttggt gtaattttga ctccgtgccg gcgtggtgaa attggtagac
     2821 gcagaggact caaaatcctc cgggggcaac tccatgtcgg ttcgattccg accgccggca
     2881 ccaataaaaa agatcgggta gtttccgatc ttttttgctt tcaggcggat ggaatcgaac
     2941 caggggtatg ggggaaggat tcccccatgg gtcaaggtgc ctcgtccatt gccgcacagc
     3001 gcctatttcg gagattgttg tttctttacc cactccccaa tttttatatt gatctccggg
     3061 acggcctttt gcgctgcctc gatcccggcc tgcatgcagc gcttcttctg ggaaaaatca
     3121 agcatggcca cgttgcccac ggccggcgtg atgagcacat ccgcctcttt cttcctgtac
     3181 ttgacgtttt cgtcgaacat gatattcacg gcctggacca tcacgtcaac gacgtttgta
     3241 atgttgtagt tctgcacgtt ttcgctgata tccaccgcga tcacgatatc ggcgcccttc
     3301 tctcgtgcga cggcgatcgg gatattgtcc accaccccgc cgtctacgag cagccggttt
     3361 tgatggttca cgggctcgaa cacgccgggg atggcgcagc ttgcatggac tgcctttgcc
     3421 accggtccgc tgtcgagcac gacgcgcgtg ccgcggttca gatcggttgc taccgcggcg
     3481 aaaggcagct tcaggttttc gatgttcttc gtcgttatct tcgttcttac gaagtcctcg
     3541 agtttttcgc ccttggccat gcccatgccg gtgatcgcgt tcaggatccc gtaatcgaag
     3601 agggtgtctt tttccagcgc gaacgccgtc cattcaagct cgaaacagtt ctgatcattt
     3661 gcatagatcg cgccgatgag gctgccgacg ctcgtcccga tgatcatgtc gatcgggatc
     3721 ttctcctgct cgagcgccct gatcacgccg acatgcgcga acccccgtgc agcacccccg
     3781 ccgagcacga gtgctatcct cggtttcggc ggctccgtca gaggcggcgg cgcctttggt
     3841 gaaacacagg acgtaataat gctcataacc attacgattg ccatcaatct catctgcttc
     3901 ataagtccct cacgaacctg cccttgaatg ggttgccctt cgaggtatcc tcatttcttc
     3961 tttttgtgcg aattttcaat ttcattccgc ttcgtcgaga aacccagggg agttaactct
     4021 tcctcttttt ttacgagggc cataaaagcg catttcgtgc agtctttcag cttctctgca
     4081 tacgttccct ggatcttacc gccgcagaag gagcccacga ccgcccagca tgccctcccc
     4141 gcgccgattc ctccatagac gccggacaat tcctcatcac tggtcaccgg gcatattccc
     4201 agttcccccg ccttatctcc accaggctgc cgtccgcagt gcgtgaactc ccagcaattc
     4261 agctttttca tggcgtcctc cctgtttcag aaagggattg cggacccgcc caatgcaggg
     4321 ccggcgggaa cgacggaaac cggcaggata gctgatccgg tgaattattg tatacctcga
     4381 gggtctccgc ggtcaagtga cgaaaatcac atctcctgcc ttccgggtaa cgtgaaagaa
     4441 gagttgcggt cgcgcgcgaa acagggtaga gtgcggatcg atcgtgcctc cgcgatcctt
     4501 ttcgtatgac gctctgccgt gaaatcaaac acaccctctc cggcgccaag cagacctatg
     4561 cctgcgaact gctggagtac gagccgggca tcggcatgct tcggtacgtc gtcgaccgag
     4621 agtacgtcat caacggacgc cggctcctgc ccggcgacgt cacgcacgcc ctgtattggg
     4681 aggaccggcc gtataccttg tacgcatggg acctgggcgg ccggggaggc aggctcttct
     4741 acttcaacat agccgactcc atcacgctcg gacgcgagga attcgtctgg cgcgatctgt
     4801 cgatcgatgt cctgatcgac gccgcaggcg gcattgatat cctggataaa cacgagctcc
     4861 cacccgatgt ggccccggaa ctcaggcgct atatcgacga ggccgtggcc ctggtcgtca
     4921 ggcggcacgg agagatcatc agggaggcga gtgagcgaat cgggcggcac ggaaggtgac
     4981 tgcggggtcc gggtctcaag gacagggcgc ctctcggagc acgcgggaga cgcggaatga
     5041 cattatggga aaaggaacag gagaatgg
//
LOCUS       DASSLD010000499         4085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_759282,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000499 DASSLD010000000
VERSION     DASSLD010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759282"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VFK23_09020"
     CDS             <1..616
                     /locus_tag="VFK23_09020"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HET7319263.1"
                     /translation="VFAVGTQQVQLGTSASIEGGVLADLVDGMKVEAEGHNVANGVLT
                     AEKVTIKDNVRFETNAEAAGSANVLGKTVTFTSGTRLSNLASSASITVGDGLRIRGFL
                     NRDGSTITATRVEKLSNPVQSNKIIVQGPVKNIDSTGHTFAIMGITISAGASTARPND
                     DTGSETLTMPLADFFAALTADRTIVKARGTFSGGTLTATEIELE"
     gene            828..1598
                     /locus_tag="VFK23_09025"
     CDS             828..1598
                     /locus_tag="VFK23_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319264.1"
                     /translation="MKLNVMVLVLFIFVAAAASAQAVQTQITVRVKSKDAKFVGTSMG
                     GALITIRNVQTGELLAKGYTSGGTGNTARIMKTPVSRGMTLSDESAAAYATTIDFDEP
                     TLIEVTAYGPFAGLQSANRVSATQWVVPGKHITGGDGFLLELPGFVVNVEAPSAHSTL
                     AGTPKAVTIRANITMMCGCPITPGGIWDASKIEVAALLSRNGQRTGVLPLQYAGTPNQ
                     FTGTWTIQEAGTYEAVVYAYDASNGNTGLDRVTFMVGP"
     gene            1665..2090
                     /locus_tag="VFK23_09030"
     CDS             1665..2090
                     /locus_tag="VFK23_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319265.1"
                     /translation="MRTASVRWAVLTLVSALCLLAAGRPSQADIEWTEKKQFKLDVSP
                     IDMATSPDGKWVIFLSRGELLVYSVPDDRIAGRTAVDKAFDKLTYSAVDDTVILSSST
                     AKTLKFIQVEAVYKFSLAGLPFKGPENAPVTVVVFSDYQ"
     gene            2103..2522
                     /locus_tag="VFK23_09035"
                     /pseudo
     CDS             2103..2522
                     /locus_tag="VFK23_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            2741..>4085
                     /locus_tag="VFK23_09040"
     CDS             2741..>4085
                     /locus_tag="VFK23_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319266.1"
                     /translation="MGSRLRGIFLLLFALSGFSGLIYESIWTDYLKLFLGHAAYAQTL
                     VLAIFMGGMAIGSAISSRYSLRWKNLLLCYAVTEGIIGFCALPFHAAFDRTINYSFTT
                     IIPHLNNPVAVNAFKGTISALLILPQSVLLGMTFPLMSAGILRLFPDKPGSTVAMLYF
                     TNSIGAATGVLVSGFVLVRLVGLPGTMAVAGLINITLAVIVWFLLKMPEAHGTITISK
                     GVTPSLGSGRKRSKAKTKTPPATEGSLGRMEVPDLGVTATKSSTERGPIQARPSWYGF
                     FLLASLVTGAASFIYEIGWIRMLSLVLGSSTHSFELMLSAFILGLALGGLWIHRRIER
                     AASPVRFLALVQVSMGLLALLTLPLYGSTFPLMQWLVKTLEKTDAGYALFNLSGSAIA
                     MFVMLPATFCAGMTLPLITFILIRGGHGERSIGAVYSANTVGAIIGVFFAVHLGMP"
BASE COUNT          886 a         1153 c         1182 g          864 t
ORIGIN      
        1 cgtcttcgcg gtaggaaccc agcaggtgca gttggggacg agcgccagca tagaaggcgg
       61 ggtgctggcg gacctggtgg acggcatgaa ggttgaagcc gaaggccata atgtggcgaa
      121 tggtgtcctg acagccgaaa aggtaacaat caaagacaac gtccgcttcg agacgaatgc
      181 cgaggcggcc ggttcggcca atgtccttgg aaagaccgtg acattcacct cggggactcg
      241 tttgtccaat ctggcaagca gcgcttccat tacggtggga gacggcctca ggatccgcgg
      301 tttcctgaac agggatgggt cgaccatcac ggccacacgg gtggaaaagc tcagcaaccc
      361 ggttcagagc aacaaaatca tcgtccaggg gccggtgaaa aatatagact ccacagggca
      421 taccttcgcg attatgggca taacgatcag cgcaggagct tcaaccgcgc ggccgaatga
      481 cgacacaggt tcggaaacgc tgacaatgcc gctggccgac ttcttcgcag ccctcacggc
      541 ggatcggacc atcgtcaagg cgagggggac tttttccgga gggacgctta ccgcaacgga
      601 aatcgaactc gaataaccgc attctccctg aatggcggcc gaaaggccgc cttttttttg
      661 cctttccgcc ggggcatttg catcaagccg ggtcggatgc acgggctgat gatggtccac
      721 agcaggcctg tgagtggtgc ttaatgatgt attttatggt atagttgttt tcgtgatttc
      781 ccggcgcatg agtatgccgg gaacggggcg aaaggagcag ttgtacgatg aaacttaacg
      841 tcatggtcct tgtactgttc atttttgtcg cggccgctgc atccgcgcag gcggtccaga
      901 ctcagatcac cgtgcgtgtg aaatccaaag atgctaaatt cgtgggcacc agcatgggag
      961 gggccctgat aacgatcagg aacgtccaga ccggcgagct tctggcgaag ggttatactt
     1021 cgggagggac aggcaacacg gcaagaatca tgaaaacgcc ggtgtcgcgc ggcatgacgc
     1081 tttccgatga aagcgccgcg gcatacgcga caactatcga tttcgacgag ccgacgctta
     1141 tcgaggtgac cgcctacggt cctttcgcgg gcctgcagtc cgccaacagg gtgtccgcca
     1201 cccaatgggt agtcccggga aagcatatca ccgggggaga cggctttctc ttggaactgc
     1261 cgggtttcgt ggtgaacgtc gaggcacctt ccgcgcattc gacgctggcg gggacgccga
     1321 aagccgtcac gataagggcc aatattacga tgatgtgcgg gtgtccgatc acaccgggcg
     1381 ggatctggga cgcaagcaag atcgaggtgg cggccctctt gagcaggaac ggacagcgca
     1441 ccggcgtttt gcctctgcag tacgccggga cgccgaacca gtttacgggg acctggacta
     1501 ttcaagaggc agggacctat gaagcggttg tgtatgcata cgatgcctcc aatggcaaca
     1561 ccggtcttga ccgcgtcacc ttcatggtcg gtccgtaagt ccgttcatcc gcgataagcc
     1621 ccggccgcca acggcaaatc gaatcccgag gaaaggatga aaacatgaga acggcatcag
     1681 tacgatgggc agtgttgaca ctcgtgtctg ccttgtgcct gctcgccgcc ggccggccct
     1741 cacaggcaga tatcgagtgg acggagaaaa agcagttcaa actcgacgtt tcaccgattg
     1801 atatggcgac ttcgcccgac ggcaaatggg tgatcttcct gtcccgcggg gagttgttgg
     1861 tgtattcggt tcccgatgac aggattgcgg gccgcacggc cgttgacaag gccttcgata
     1921 aactcacgta ttccgcagtg gatgacacgg tcatcctgtc cagcagcacg gcgaaaacgt
     1981 tgaaattcat tcaggtggag gcggtctaca aattttccct tgcggggctt cccttcaagg
     2041 gcccggagaa cgcccccgtc accgtcgtcg tattcagcga ctaccagtga ccatactgcg
     2101 cgcggcttga gccgtacctc cagcagttgc tggagaaata ccccaaagac gtcaaactgg
     2161 tcctcaagaa cttccccctg cccctgcatc cgttcgcgcg aaaggccgcc gctgcggcgc
     2221 ttgcggcgaa caaccagggg aaatactggg aattcagcgc caggcttttt gaaaattatt
     2281 cgagtttgag cgacgaaaag attcaggaca tcgccgggca actcggcctg gacagggaaa
     2341 aattcaatca tgatatgaat gatgcgtcga ttcagtccct catcaacagg gacctgagcg
     2401 acgccgaaca ggtgcccgtt caggggaccc ctacgatttt cgtcaacggc aaggccttga
     2461 agaaccgcgg accgaacgaa tttcagcaga tgatcgagca cgagttgaag aaaaagaagt
     2521 aggggcggag agaatgattt accgaatatt tggctaaagg gtgttcgaga gatttccgtg
     2581 cacaggtccc gacgggaaag aggaaccgat tcacgtatgc gccgatctgg acgatcctgc
     2641 tgccggaaat gcgaaacgac cgcgcttatt cattatcatt ccgatgacct ctgaaatgaa
     2701 accggcaatg ccgtaagtct cgcaatgagg tgtcaatcac atggggtcaa gacttcgcgg
     2761 catctttttg ctgttgtttg ccctgtccgg attctccggt ctcatttatg aatcgatctg
     2821 gaccgactac ctcaaacttt ttctcggaca tgccgcatac gcccagacgc tcgtgctggc
     2881 aatctttatg ggggggatgg ccatcggttc ggccatctcc agcaggtatt cgctgcgctg
     2941 gaaaaatctg ctgttatgct acgccgttac cgaagggatc atcggattct gcgctcttcc
     3001 gtttcacgcc gcatttgatc ggaccattaa ttactcgttc acaacgatca tcccgcactt
     3061 gaacaaccct gtcgcagtca acgccttcaa agggacgatc tcggcgcttt tgatacttcc
     3121 gcagtccgta ctgctcggca tgacctttcc cctgatgagc gccggcatcc tccgcctgtt
     3181 tcccgacaaa ccgggcagca ccgttgcaat gctctacttt accaacagca tcggtgcggc
     3241 aacgggtgtc ctggtgagcg gttttgttct cgtccggctt gtcggcctgc cgggcaccat
     3301 ggccgtagcg ggcctcatta acatcaccct cgccgtgatt gtctggttcc tgctgaaaat
     3361 gccggaagct cacggaacaa tcacaatctc gaaaggagtc accccttcac tgggatccgg
     3421 acggaaacgg tcgaaggcaa aaacaaaaac cccgcctgcg accgagggat ctttaggcag
     3481 gatggaagtc ccggatctcg gggtgaccgc gacaaaatca tcgaccgaac gcgggccgat
     3541 tcaagcgcgc ccgtcatggt acgggttttt cctccttgct tccctggtga cgggtgccgc
     3601 ttccttcatc tatgaaatcg gctggatcag gatgctgagc ctcgtcctgg gaagctccac
     3661 ccattcattc gagctgatgc tcagtgcctt cattctcggc ctggcgctgg gcggcttgtg
     3721 gatacatcgg cgcatcgagc gtgctgccag tcctgttcga ttcctcgccc ttgtgcaggt
     3781 cagcatgggg cttctggccc ttttgacgct gccgctctac ggctccacgt ttcccctcat
     3841 gcaatggctc gtcaaaactc tcgaaaagac ggacgcgggc tacgcgctct tcaacctctc
     3901 cggcagcgcc atcgccatgt tcgtcatgct gccggcgacc ttctgcgcgg ggatgaccct
     3961 gccgctcata acgttcatcc ttatcagggg aggccacgga gagcggagca tcggcgcggt
     4021 gtattcggcc aataccgtcg gggcgatcat cggcgtgttt tttgccgtcc acctcgggat
     4081 gccgc
//
LOCUS       DASSLD010000500         5036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_400409,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000500 DASSLD010000000
VERSION     DASSLD010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400409"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VFK23_09045"
     CDS             complement(<1..716)
                     /locus_tag="VFK23_09045"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET7319267.1"
                     /translation="MPFFTGEIFVSEVYNKPVLDPMGEEIGRLTDIILMMGEPFPAVS
                     FLFISAGKETFLVPWEQVNLFNRRVISVSVQRKDLAPASPSPSDILICRDLLDKQIVD
                     INGAKLVRVNDLELGGVNGKLCLVAADFGFRGLLRRLGVEKRGEKVFSLLHAGLPHKL
                     ISWRLVQTVEPKLAKLTLTVTRQKVSLLHPADLAEIISEVSQKERTALFGSLDVDTAA
                     EALHELEPHVQVDIIDDMSK"
     gene            1023..1625
                     /locus_tag="VFK23_09050"
     CDS             1023..1625
                     /locus_tag="VFK23_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319268.1"
                     /translation="MSEPTVKDVKKDIYLAMHKAQSMLELTEDGFMKSKASSLDRADE
                     LAVEIQAKEDNLTTLLAKMASANNEARSILTVPAHIEKIATSIKRIGENSRSRIKEGM
                     LFSDKALLETGKLFTKSKDILKRAGEAAVTGSQTTLQSVLADSDALERMANDYATAHE
                     ERLVTGECSPKSSSTYLCILYAFEDMGAHIKNTISKLSGK"
     gene            1670..2452
                     /locus_tag="VFK23_09055"
     CDS             1670..2452
                     /locus_tag="VFK23_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7319269.1"
                     /translation="MPDPSIPKIGLALSGGAARGIAHVAVLDALEMEGIPIHAIAGTS
                     AGSVVGALYAAGMPVSEIKRIILKTTWTDIIPFRLPRRGGLISSEGIYRFMDSILPVK
                     KFSSLLFPFAAVATDLRTGEKVSITTGSIARAVQASCSLPIVFTPTELNRRVLVDGGV
                     VSQIPVRTAREELDAKIVIAVNVNYKALETEEFDSMIKIAAHLSALWASKNARVEEKL
                     ANVLINVNVKGISLYDLSKAKELLKRGRAAVEEKLPEIRALL"
     gene            2764..3882
                     /locus_tag="VFK23_09060"
     CDS             2764..3882
                     /locus_tag="VFK23_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7319270.1"
                     /translation="MKKRLILTMIGLAVLIAILIAVKAIQIGAMIDQGKKFVPPSETV
                     TSTVLKAESWETALTAVGTMNALQGVTVAAELSGKVVEIAFEAGTPVKKGDILLRQDT
                     SSEEAQLPGALAQVDLMRTNLERMNQLLAKSLISKADRDNAVASAEQAEAQAANIRAT
                     IAKKTIRAPFSGHLGIRQVNLGQMLREGDPIVTLQSLDPIYVDFTLPQQQLHMVRLGL
                     PVRVTTDALPGETLQGRITAINPLVEAETRQIKIQATVANRGNKLRPGMFGNVAVGLP
                     ELQKVLAIPATAVLYAPYGDSVFVIEDAADKKGKVLRQQFVRLGPKRGDFVAVTSGLR
                     EGETVVSTGVFKLRNGQSVVVDNTLSPDFRKKPTPENN"
     gene            3888..>5036
                     /locus_tag="VFK23_09065"
     CDS             3888..>5036
                     /locus_tag="VFK23_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7319271.1"
                     /translation="MNITDLFIRRPVLALVVNLVIIIAGLQAIRTLNVRQFPHSENAA
                     VTVTTVYVGADANLVRGFVTAPLERAIAAADGIEYMESKSSLGLSTISVRLRLNYDST
                     KALAEISSKVDQVRRDLPPESEVPVINIESADSQFASAYLSFTSDILQQNEITDYLVR
                     VVQPRLSAVPGVQRADILGARTFAMRIWLKPERMAALNISPAQVRQALTANNFLAALG
                     SSKGALIQVNLTANTNLRTVDEFKNLAVREQSGTIVRLREIADVVLGAEDYNAEVHFS
                     GQTAVFMGIWPLPNANSIDVIRRVTVEMAAIQGSLPSGMKARVAYDATNYINNAIREV
                     LKTLGDTLLIVVVVIFLFLGSFRAVFIPVVAIPVSLIGGVFLMQAFGFT"
BASE COUNT         1079 a         1566 c         1442 g          949 t
ORIGIN      
        1 tccttggaca tgtcgtcgat gatgtcgacc tgcacatggg gttcgagttc gtgcagcgcc
       61 tccgctgccg tgtcgacgtc gagactgccg aacagggctg tccgttcctt ctgggacacc
      121 tcgctgatga tctccgcgag gtcggcgggg tgcaggagcg ataccttctg ccgggttacg
      181 gtcagggtca gtttcgccag tttgggctcg acggtctgga caaggcgcca ggaaatgagt
      241 ttgtgcggaa gaccggcgtg gagcagggag aagacctttt caccgcgttt ttcgaccccg
      301 agacgccgca agaggccccg gaacccgaag tctgcggcga cgaggcacaa cttcccgttg
      361 acgcccccga gttcgaggtc gttcacgcgg acgagtttgg cgccgttgat gtctactatt
      421 tgcttgtcga ggaggtccct gcagatcagg atgtccgaag gcgagggcga tgccggggcg
      481 aggtcctttc tctggacgct cacggatatg acccgccggt tgaagaggtt aacctgttcc
      541 cagggtacga ggaaagtttc cttgcctgcg gagatgaaga ggaagctcac cgccggaaag
      601 ggctcgccca tcatcaggat gatgtccgtg agccgtccga tctcctcgcc catggggtca
      661 agcaccggct tgttatagac ctcgctgacg aaaatttcac cggtaaaaaa gggcatgatc
      721 cgctccgcgg cacggtctct gatgaatgat ggtttaggtt ataccagcgg ccccagggta
      781 tgtcaagaaa aaaggcctct cgccgtcata gggtatgtta aaatatcttt caggggacga
      841 aaacaggccc tggaagacgg ccaatgctct tcgtcatggc acgacaaaat ctataagaca
      901 ttaaaatatc gatataaata aatttaacat gtcatgaccg gcgatttctg ctatagtttg
      961 aaaaattttt ttacgatatc gcacctgtcc ctaccgctgg acctgaaagg agctcgcacc
     1021 gtatgtcgga accgaccgta aaggacgtca agaaagatat ctatctggcg atgcacaaag
     1081 cacagtccat gctcgaactt acggaagacg gcttcatgaa gagcaaggcc agttccctgg
     1141 accgtgccga cgaactcgcc gtggagatac aggcgaagga agacaatctc accaccctgc
     1201 tcgcgaagat ggcatctgca aacaacgagg cccggtccat cctgactgtc cccgcacata
     1261 tcgaaaagat cgccaccagc atcaagcgta tcggcgaaaa cagccggagc cggatcaaag
     1321 agggcatgct gttcagcgac aaggccctcc tcgaaaccgg caagctcttc accaagtcga
     1381 aagacatcct gaaaagggcg ggcgaggccg cggtgaccgg atcgcagacg acgctccagt
     1441 ccgtcctggc cgacagcgac gcgctcgaac gcatggccaa cgactacgcg accgcgcatg
     1501 aggagaggct cgtgaccggc gaatgttctc ccaagtcgtc gtcgacctat ctctgcatcc
     1561 tctatgcctt cgaagacatg ggcgcccata tcaagaacac gatctcaaag ctctcgggga
     1621 aataagcctc attcctttcc atccgtccga aaacatccgc gggtgaagaa tgcctgaccc
     1681 atcgatcccc aaaatcggcc ttgcgctctc gggcggcgcg gcgcgcggca tagcccatgt
     1741 tgccgtcctg gacgccctgg aaatggaagg catacccatc catgcgatcg cagggacgag
     1801 cgcgggcagc gtcgtcggcg ctctctatgc cgcgggcatg cccgtcagcg agatcaaacg
     1861 catcatcctg aagacaacat ggactgacat tattcccttc cgccttccgc ggcggggggg
     1921 gcttatttcc agcgaaggca tctaccgctt catggattcg atcctgccgg ttaaaaagtt
     1981 ttcgtccctc ctcttcccct ttgccgccgt cgccaccgat ctccgcaccg gtgaaaaagt
     2041 ctccatcacc accggctcca ttgcaagggc cgtccaggca agctgcagtc tgccgattgt
     2101 ctttacgccg acggaactca acaggcgcgt tctggtggac ggtggagtag tcagtcagat
     2161 accggtcagg acggcaaggg aggaactgga tgcgaaaatc gtaatcgcgg tgaacgtgaa
     2221 ttacaaggcc ctggaaactg aggagttcga cagtatgata aagatcgcgg cgcacctctc
     2281 cgccctctgg gcttcgaaga acgcccgggt agaggagaaa ctcgcgaacg tgctcatcaa
     2341 tgtcaacgtg aagggtatct ccctgtacga cctctcaaag gcaaaggaac tgctgaagcg
     2401 aggcagggcg gctgtcgagg aaaaactgcc ggagatcagg gccctgctgt gaatcccgaa
     2461 cgatttctta cgcgacgtac cgcgccttca tcgtcatttc cctcctcaaa cgttatcccc
     2521 tatcggcccc tccccttgcc gacccgtccc aacgggctac ggcttacgtg atcatcccgt
     2581 aaaaaagcac caactgtgtc ttgataaatt tcttgtatcg tgttaacttt tactatgtcg
     2641 tgatgctccc ggtccttatt gatcccgcgc catgaaccaa ataccgggcg gacggcccca
     2701 tgggtgtcgg ggccttgccg tcgcggtccc ggaccatgac cccgtatcgg agacattctc
     2761 aatatgaaga aacgcttgat actcaccatg atcggtctgg ctgttctcat cgccattctc
     2821 atcgctgtca aggccatcca gattggcgcg atgatcgacc aaggaaagaa gttcgtaccg
     2881 ccgtccgaga ccgtgacctc cacggttttg aaggccgaat cttgggaaac agccctgacc
     2941 gcggtcggca cgatgaacgc cttacagggg gtcactgtgg ccgccgaatt gtccggcaag
     3001 gtggtcgaga tcgctttcga ggcgggcacg ccggtaaaga agggggacat cctgctgcgt
     3061 caggacacct cctcggagga ggcccagctt cccggagcct tggcccaggt ggacctgatg
     3121 cgcaccaacc tcgagagaat gaaccaattg ctcgcgaaga gcctgatctc gaaggccgac
     3181 cgcgataacg ccgtggcgag tgcggagcag gcggaggccc aggccgcgaa cattcgggcc
     3241 accatcgcca agaagacgat ccgcgccccc ttcagcggcc atctcggcat ccgacaggtc
     3301 aacctgggcc agatgctccg cgagggggac cccatcgtca cgctgcagtc cctcgacccc
     3361 atctatgtcg atttcaccct gccccagcag caactccaca tggtgcgcct tggcctgccc
     3421 gtgagggtaa ccaccgatgc cctgccgggg gagacgctgc agggccggat caccgcgata
     3481 aaccctcttg tcgaagccga aacgcgccag atcaagattc aggcgaccgt ggccaatcgc
     3541 ggcaacaagc tccgccccgg catgttcggc aacgttgcgg tggggctccc cgagctgcag
     3601 aaagtgctcg ccatccccgc gacggccgtt ctgtacgccc cctatggaga ctcggtcttc
     3661 gtcatcgagg acgccgcgga taaaaaggga aaggtgctgc gccagcagtt cgtgcggctc
     3721 ggtcccaaac gcggcgactt cgtcgctgtc acgagcgggc tccgggaggg ggaaacggtc
     3781 gtgagcaccg gcgtcttcaa actgcgcaac ggccagtccg ttgtggtcga caatacactg
     3841 tcccccgact tccggaaaaa gcccacaccg gagaataact gacttccttg aatatcaccg
     3901 acctcttcat acgccgtccc gtcctggcac tggtggtcaa cctggtcatc atcatcgccg
     3961 gcctgcaggc gatccgcacc ctcaacgtgc gccagttccc ccacagcgag aacgcagcgg
     4021 tcaccgtcac cacggtatac gtcggcgctg atgccaatct ggtgcgcggc ttcgtcaccg
     4081 cgccgctgga gcgcgccatc gccgccgccg acggcatcga atatatggag tcgaagagca
     4141 gcctcggcct ttccaccatc agcgtgcggc tcaggctgaa ttatgactcc accaaggcac
     4201 tggccgagat cagctccaag gtcgaccagg tgcgccgcga cctgcctccc gagtccgagg
     4261 tgccggtgat caacatcgag tcggcagaca gccagttcgc ctcggcatat ctgagcttta
     4321 cctccgacat cctgcagcag aacgagatca ccgattacct cgtgcgcgtg gtgcagccgc
     4381 gcctgtcggc agtccccggg gtgcagcgcg ccgacatcct cggggcccgc acctttgcca
     4441 tgcggatctg gctcaaaccc gagcggatgg cggccttgaa catcagcccc gcccaggtgc
     4501 gccaggcgct gaccgcgaac aacttcctgg ccgccctcgg cagcagcaag ggggcgctca
     4561 tccaggtcaa cctgaccgcc aataccaatc tgcgcaccgt ggatgagttc aagaacctgg
     4621 cggtgcggga acagagcggc accatcgtga gactgaggga gatcgccgac gtggtgctgg
     4681 gcgcagagga ctacaacgcc gaggtgcact tctcggggca gacagccgtg ttcatgggga
     4741 tctggcccct ccccaacgcc aattccatcg acgtcatccg gcgcgtgact gtggaaatgg
     4801 cggcaatcca gggaagcctg ccgagcggca tgaaggcacg cgtggcgtac gacgcaacca
     4861 actacatcaa caacgccatc cgcgaggtgc tcaaaaccct cggcgacacg ctgctgatag
     4921 tcgtggtcgt catcttcctt ttcctcggct ccttccgcgc ggtgttcatc ccggtggtgg
     4981 cgattccggt atcgctcatc ggcggcgtct tcctgatgca ggccttcggc ttcacc
//
LOCUS       DASSLD010000501         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_610935,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000501 DASSLD010000000
VERSION     DASSLD010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610935"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="VFK23_09070"
     CDS             complement(<1..1395)
                     /locus_tag="VFK23_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319272.1"
                     /translation="MKIFYPILCLLLVFAAVSSAGSAEQVESTVPCPPGTGQAEGSGG
                     ICSDPGQAMLPLVQPGSGILQPLPGVQRSVATGTGIKKPDRPSLKSPEKGTVVVYFFW
                     GRGCPHCEKEEAFLAELKGRNPALVIRDYEVWYDKKNAGLLSSFLQARGMLSSGVPVT
                     FVDKQVFMGFARQTQDRIAAAIQMCLSGACEDPAAMMTRPDAADRAVPSRQMTSGMTA
                     TVPAGEKGETVDIPLLGTLDTRTTSLPVLTFFIAGLDSFNPCAFFVLLSLLGLLVHAQ
                     SRNKMLLIGGVFVFFSGFIYFLFMAAWLNLFLVMGHVTVITTIAGGVSILIAGINIKD
                     FFFFKQGVSLSIPDSAKPKLFDRMRRLLRSTSMFSMLIGTTVLAIVANSYELLCTAGF
                     PMVFTRILTLNALSLPSYYLYLVFYNVVYIIPLLVIVLAFSITLGSRKLSERQGRLLK
                     LLSGLMMLGLGVVLI"
     gene            complement(1592..1774)
                     /locus_tag="VFK23_09075"
     CDS             complement(1592..1774)
                     /locus_tag="VFK23_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319273.1"
                     /translation="MDIAKAAQMGDTTLEVNGLRIFLEEKANSMLQNTSIDFQENQGF
                     VLTGMQPSACGSSCSC"
     gene            complement(2053..>2357)
                     /locus_tag="VFK23_09080"
     CDS             complement(2053..>2357)
                     /locus_tag="VFK23_09080"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HET7319274.1"
                     /translation="AVEVMKEIGIDISRQKSKEIDEALVRKMDIVVTLCTSAEEACPR
                     TPPEVERLHWPIRDPVGARGSREEVLKEFRRARDEIKGRIEELVETIRNRKKAEIS"
BASE COUNT          529 a          675 c          628 g          525 t
ORIGIN      
        1 gatcaacacg acgcccagtc cgagcatcat gaggccggat agaagtttca gcagcctccc
       61 ctgccgctcg gaaagttttc tgctgccgag ggtaatgctg aacgccagga cgattacgag
      121 gagcggtatg atgtagacga cattgtaaaa aacgaggtag agatagtagg acggcaggga
      181 cagcgcgttc agcgtcagga tccgcgtaaa caccatcgga aatccggcgg tgcacagaag
      241 ttcataggaa ttcgccacaa tcgcaaggac cgtcgtcccg atcagcattg agaacatcga
      301 cgtggacctg aggagtcttc tcatccggtc gaagagcttc ggtttggcgc tgtccgggat
      361 cgacagagac actccctgtt tgaagaaaaa aaagtccttg atgttgatcc cggcgatcag
      421 gatcgagacc ccgcccgcga tcgtcgtaat gaccgtgaca tggcccatga cgaggaacaa
      481 attcaaccag gcggccatga acagaaaata gatgaacccg gaaaagaaga caaagacacc
      541 gcctatgagc agcattttgt ttcgcgactg ggcatggacc aggagcccca ggagggagag
      601 aagcacgaaa aaggcgcagg gattgaagct gtcgagccca gcgataaaaa aggtcaggac
      661 gggtagcgac gtggtcctgg tgtcgagcgt gccgagcagt ggtatgtcta cagtctcgcc
      721 cttctccccg gccgggaccg tggccgtcat gcccgatgtc atctgacgcg acgggacagc
      781 cctgtccgca gcatccggcc gagtcatcat cgcagcggga tcttcgcatg cccctgacag
      841 gcacatttga attgccgcgg cgatccggtc ctgcgtttgc cgtgcaaagc ccatgaagac
      901 ctgtttatcg acaaaggtta cgggtacgcc ggaagagagc atgcccctcg cctgaagaaa
      961 tgaggacaag agaccggcgt tcttcttgtc gtaccagacc tcgtaatcac gtatgaccaa
     1021 ggcgggattc ctgcccttta attcggcgag aaaggcctcc tccttttcgc agtgggggca
     1081 tccccgaccc cagaaaaaat agacaacgac cgttcccttt tccgggctct tgagagatgg
     1141 cctgtccggc tttttgattc ccgtgcccgt cgctacgctc ctctgcactc ctggaagagg
     1201 ctgcagaatg cctgagcccg gctgtacaag tgggagcatt gcctggccgg ggtcgctgca
     1261 aatgcctccg ctgccctccg cctgcccggt tccgggcggg cacggaaccg tcgattccac
     1321 ctgctccgca gagccggctg atgaaacagc tgcgaacacc agcaataaac agagtattgg
     1381 ataaaagatt ttcattcggc acttcctgag tcgttgcctt ggatgttttg tacaacacga
     1441 ccatatctcg gggcaactta ccggcaggag actaggaata ttgtagcaaa agaacgcacc
     1501 cccggaagcg ggcgagaggc aaaacgcaaa aaaaccgctg cttccggtcg gaagcagcgg
     1561 tcgatgaaac gaggctttcg ggatggtctt ctcagcagct gcaggaggac ccgcaggcgc
     1621 tcggctgcat acccgtgagt acgaaaccct ggttctcctg aaaatcgatg ctcgtattct
     1681 ggagcatgga attggctttc tcctccagaa agatcctgag tccgttcact tcgagggtcg
     1741 tgtcacccat ctgggcggct ttggctatgt ccatagccag tgaagggctt cagccccccg
     1801 ggacgacgaa gattcttacg ccgtccccgt tgctgccttg tttggctacg acctctttga
     1861 atttaactat cgcactgtcc gtcaaactta ccatacttcc tccttgccgt tatctgtata
     1921 aacactgttc tctgttaagg cttcgtaaat ttttctataa taccagtctc gcgccggttg
     1981 cgcaactgtt ttttctctct gtttattata acataacact gttttaaacg gagaaggccc
     2041 ttacgggcaa atttacgata tttctgcctt ttttctgttc cttattgtct ccacgagttc
     2101 ctcgatcctt cccttgattt cgtcccgcgc ccgcctgaat tccttcagca cctcttccct
     2161 gctcccccgg gccccgaccg gatccctgat gggccaatgc agtcgctcca cttccggagg
     2221 ggtccgcgga caagcctctt cggcgctggt gcagagggtg acgacgatgt ccatcttgcg
     2281 cacgagggcc tcatcgatct ccttagactt ctgtcgtgat atatctatgc cgatttcctt
     2341 catcacctca acggccc
//
LOCUS       DASSLD010000502         5280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_497957,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000502 DASSLD010000000
VERSION     DASSLD010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497957"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VFK23_09085"
     CDS             <1..454
                     /locus_tag="VFK23_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET7319275.1"
                     /translation="RNCQHLIELAKMIDIPVVVTEQYPKGLGRTVPELQSVLPEYRPV
                     EKTSFDCCGEQSFFREFHMHGRKTAVLTGMETHICVLQTCVGLLKSGINVHVVEDAVC
                     SRTAENWRTGIEFMRDAGAVVTCTETALFQFLKVAGTEEFKKISQRIK"
     gene            558..2207
                     /locus_tag="VFK23_09090"
     CDS             558..2207
                     /locus_tag="VFK23_09090"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7319276.1"
                     /translation="MAQQEIIPDDVKQAIKETLLDGLKEDVAIEVFTKPGQNDQFNDA
                     AAALVRGIAELSEKLKASFHNIGDELSVKRNVTRSPTVLIDPDKYRIRYTGAPVGEEG
                     RSFLVALLMASSGKTVLTEEALAKIKDQLREKRDIQVFVSPTCPYCPQQVMTAVSAAI
                     AKPDLISAEVIEIYENQDLAESLGTLAVPQTFINNTFTGAGLQPEPLFMESLLSLKEP
                     QIASTQISGEPVEKDLVIIGGGPAGLTAAIYAVRAGLNSIVIEKSNLGGQVTITPVVE
                     NYPGFMRIGGKSLVDLIAQQAAQYCEIHIGEYVTEVSHERKDGRFTLKTNHAIYNTKG
                     MIIATGVANRALEAKGSQKFYGRGVSYCATCDGYYFKDGKTVIVVGGGNTALTDALYL
                     HNIGAKVTLVHRRGDLRAERRLQDSFQQSGIPALWHSEVREITGDKIVKAVKIEDAKS
                     GKMKELPVEGVFVAIGYVPNNDLAKQLGLEIDDQGYIKTDLMTMRTSLPGVYAAGDIT
                     GAPKQIAVAVSHGSIAAMTAFEDLAAPAGMKQEKVVSSTTT"
     gene            complement(2338..2913)
                     /locus_tag="VFK23_09095"
     CDS             complement(2338..2913)
                     /locus_tag="VFK23_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET7319277.1"
                     /translation="MNNGAKTGLIFLGVIVLIVLVAVVYGMGEYNKVVAMDEQVKSQW
                     AQVENQLKRRYDLIPNLVETVKGYTKHEQEIFEHLADARTKYFQATTVKDKIQASQQL
                     EGFLSRLLVLQETYPQLKANENFLKLQDTLEGTENRISVERKRYNDDVRALNTYRRTL
                     FGRFFASWAGVNEAQYYEIPQAEKEAPKVKF"
     gene            complement(3051..3827)
                     /locus_tag="VFK23_09100"
     CDS             complement(3051..3827)
                     /locus_tag="VFK23_09100"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET7319278.1"
                     /translation="MVRRVFIAVFLAAFCASLAAASTPEPPAVPRDYVVDLAGVVPDE
                     AQARLNAYLRELEQKTTAQVLVLTVQSLDGEEIRSFGLKIAERWKLGQKGRDNGVLFV
                     VAVQDRKYTIEVGYGLESVLPDSAVGTIGREYLVPYFKKGDYGAGIYAAVLAAANTIA
                     SQEGVTITGMPALQRPRELPAGRPFGILHAAAFAVLLVVALILFITHPRQCFLLLLMS
                     QMGGGRGGWAGGGGGFGGSGGGFGGGGGGGFGGGGASGGW"
     gene            complement(3820..4632)
                     /locus_tag="VFK23_09105"
     CDS             complement(3820..4632)
                     /locus_tag="VFK23_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319279.1"
                     /translation="MKSNATLRFIVQVRLLCYNSATMNHLDFTGLKPIVAEKLSPMVE
                     DILKGHASNLHSFHVVGSAVIPDYDEKLSDINSVVVLHSMDLRFISFLAPLGKKYGGK
                     RIAAPLVMTPEYIMSSLDAFPVEFLDFKLIHKTVYGGDILRGLELDGKNLRLQCEREV
                     KTKLIQARQGYIASLGKKDLLGAALVRSITGSMAFFRAVIVLLGKEPPVPRSDVITVL
                     GAATGIDTGIFQTLLALKAGLIKPKEHELHTLFEQYYQALESTEKIIDAIHG"
     gene            4604..5101
                     /locus_tag="VFK23_09110"
     CDS             4604..5101
                     /locus_tag="VFK23_09110"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HET7319280.1"
                     /translation="MKRSVALLFTGNCDILEIFTRQQFSWPPIQQGDVDVVHAVLRFI
                     RRSGKAYRGAMESLQHILQSVILAISIAGAVVVIWGIAEAVVAFFLMKIGRTGKEAVQ
                     ASEAIRLRLGAHLLLGLEIFIAADIISSVATPTWERVGILASIVAVRTVLSYFLRMEM
                     KQGAE"
     gene            5128..>5280
                     /locus_tag="VFK23_09115"
     CDS             5128..>5280
                     /locus_tag="VFK23_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HET7319281.1"
                     /translation="MDPFEKKIAGTTIRLVSADLTERDVDAIVNAANSQLRHGGGVAG
                     AIVRKGG"
BASE COUNT         1100 a         1557 c         1585 g         1038 t
ORIGIN      
        1 caggaactgc cagcacctga ttgagcttgc gaagatgatc gatatcccgg tcgtcgtgac
       61 cgagcagtat cccaaggggc tgggaaggac cgtgccagaa ctccagtcgg tcctgccgga
      121 gtacagaccg gtcgagaaaa catccttcga ttgctgcggc gaacagtctt tctttcggga
      181 attccacatg cacggcagga agaccgctgt cctgaccggg atggagaccc acatctgcgt
      241 gctccagacc tgcgtggggc tgcttaagtc cggcataaat gtacacgtcg tagaggacgc
      301 cgtatgttcc cgcacggcgg agaattggag aacgggcatc gagttcatgc gggacgcggg
      361 cgcggtcgtg acctgcaccg agaccgccct gttccagttc ctcaaggtcg ccggcacgga
      421 ggagttcaag aagatatcgc agaggataaa atagacgaag gacgacacgg cgatgggggg
      481 agtgggagac gcggcgagaa aagctccgtg tctccgtgtc gcccagtctc cgcgtcgtac
      541 acttaacgag gtaccccatg gcacaacagg aaatcatccc cgacgatgta aagcaggcca
      601 tcaaggaaac gcttctggac gggctcaagg aagacgtcgc catcgaggtc tttaccaagc
      661 cgggacagaa cgaccagttt aacgatgcgg ccgctgctct ggtcaggggg atcgcggagc
      721 tttccgaaaa actgaaggcg tcgttccaca acatcggcga cgaactgtcg gtgaagcgga
      781 acgtcacccg ctcgcccacg gtcctgatag atcccgacaa atatcgcatc cgctacaccg
      841 gcgcgccggt aggcgaagag ggccgctcct tcctggtggc cctcctcatg gcgtcgagcg
      901 ggaagaccgt tctgaccgag gaagcactgg caaagatcaa ggaccagctc cgggaaaaac
      961 gggacatcca ggtcttcgtg agcccgacgt gcccttattg cccgcagcag gtcatgaccg
     1021 cggtctccgc cgcgatagca aaacccgacc tgatctcggc cgaggtcatc gagatttacg
     1081 agaaccaaga ccttgccgag agcctgggta cgctcgcggt gccgcagacg ttcatcaaca
     1141 acacgttcac cggcgccggt cttcagccgg agcccttgtt catggagtcc ctgctttcgc
     1201 tcaaggagcc ccagatcgcg tcgacgcaga tttcgggcga gccggtggaa aaggacctcg
     1261 tcatcatcgg cggaggcccc gccgggctca ccgcggcgat ctatgccgtg cgcgcgggcc
     1321 tgaacagcat cgtgatcgag aagagcaacc tgggcggcca ggtgacgatc acgccggttg
     1381 tcgagaatta tccgggattc atgcgcatcg gcgggaagag cctcgtggac ctgatcgcac
     1441 agcaggcggc gcagtactgc gagatccaca tcggcgaata tgtgaccgaa gtgtcgcatg
     1501 aacgcaagga cgggcggttc acgctcaaga cgaaccacgc catctacaat acgaaaggaa
     1561 tgatcatcgc cacgggcgtc gccaaccgcg ccctcgaagc gaaggggtcc cagaagttct
     1621 acgggagggg tgtctcctac tgcgcgacct gcgacgggta ctacttcaag gacgggaaaa
     1681 cggtgatcgt ggtaggaggc ggcaatacgg ccctcacgga cgcgctttac ctccacaata
     1741 tcggcgcgaa ggtgaccctc gtccaccggc gcggcgatct tcgcgcggag cggcggctcc
     1801 aggacagctt ccagcagtcc gggatcccgg ccctgtggca cagcgaggtg cgggagataa
     1861 ccggcgacaa gatagtcaag gccgtgaaga tcgaggacgc aaaatcgggc aaaatgaagg
     1921 aactgcccgt ggagggcgta ttcgtcgcca ttgggtacgt gccgaataat gatttagcca
     1981 aacaactcgg cctcgagatc gatgatcagg gatacatcaa gaccgacctt atgaccatgc
     2041 ggacatccct gcccggcgtc tatgccgcgg gagatatcac cggcgccccg aagcagatcg
     2101 ccgttgccgt gtcgcacggc tccatcgccg ccatgacggc cttcgaggac cttgcggcac
     2161 ctgcggggat gaagcaggaa aaggtagttt cctcaacgac aacttaggcg ggttgacccg
     2221 tagttcctcc cccttcaagg gggaggttag gtgggggatg gggtttattg aagctgagtg
     2281 cttccttcgt gacccatccc gaccccagcc ctccccttga agggggaggg agattcgtta
     2341 gaacttcacc ttcggtgctt ccttctctgc ctgcggaatt tcataatact gcgcttcatt
     2401 cacgcccgcc caggatgcaa agaaccttcc gaatagagtt ctccggtagg tattgagcgc
     2461 cctcacatca tcgttgtacc gtttgcgctc gacggagatc ctgttctcgg tcccttcgag
     2521 ggtatcctgc agcttcagaa agttctcgtt tgccttgagc tgcggatagg tctcctggag
     2581 gaccaggagg cgagagagaa acccttcgag ctgctgggac gcctgaattt tgtctttcac
     2641 ggtcgttgcc tggaaatatt ttgtcctggc atcggcgaga tgctcgaata tctcttgctc
     2701 gtgttttgta taccccttca ccgtttccac gagattcggg atcaggtcat agcgccgctt
     2761 gagctggttc tcgacctggg cccactgcga cttcacctgt tcgtccatgg ccacgacctt
     2821 gttgtactcg cccatgccgt acaccaccgc cacgagcacg atcagcacaa tgacgccgag
     2881 aaagatcaga cccgtctttg ccccgttgtt catgatgacc tcctgtagtt aagtatgaat
     2941 gggcgacagg gcgacacggc gacaagggga catgaagaac gaatcttggc tttttctccc
     3001 attctccccg tcaccccgtc tcctcttcgc cccgtcgctg tttcgtgttt ctaccatcct
     3061 cccgaagccc cgccccctcc gaaaccgccg ccaccgcctc cgccgaaccc tcctccgctt
     3121 ccgccgaacc ctccgccccc gccggcccag ccgccccttc ctcctcccat ctgggacatc
     3181 aggagaagga gaaaacactg gcggggatgg gttatgaaca aaatgagcgc gacgaccagg
     3241 aggacagcaa atgcggctgc gtgcaatatc ccgaaaggcc tgcccgcggg aagttcccgg
     3301 ggccgctgca gagcaggcat cccggtgatg gttacgcctt cctgcgacgc tatcgtattt
     3361 gccgccgcga gcacggcggc gtatatcccg gcgccgtagt cccctttttt gaaatagggg
     3421 acgaggtatt cccgcccgat cgtgccgaca gcgctgtccg gcaaaacgct ttcgagtccg
     3481 tacccgactt cgatcgtata tttccgatcc tgaacggcca cgacgaagag gacgccgttg
     3541 tctctgccct tttgtccgag cttccatctc tcggcaattt tcaggccgaa actgcggatt
     3601 tcctcgccgt cgaggctttg gaccgtaagg acgaggacct gggccgtggt cttctgctcg
     3661 agctccctca ggtacgcatt caagcgcgcc tgcgcctcgt caggcaccac tcccgcaaga
     3721 tctaccacgt agtcgcgcgg caccgcaggc ggttcgggtg tcgatgcagc ggcaagggag
     3781 gcgcagaacg ccgcgagaaa gaccgcgatg aagacacgtc taaccatgga tggcatcgat
     3841 gatcttttct gtggactcga gggcctggta atactgctcg aagagcgtgt gcagttcgtg
     3901 ctccttcggc ttgatcaggc cggccttgag cgcaagaagc gtttgaaata tgccggtgtc
     3961 gatgccggtg gcggcaccca gcaccgtaat cacgtcagac cggggcacag gcggttcctt
     4021 gcccagaaga acgatgaccg cccggaagaa ggccatggac ccggtgatgg acctgacgag
     4081 ggccgctccc agcaggtcct ttttcccgag ggacgcgatg tagccctgtc ttgcctggat
     4141 cagcttggtc ttgacctcgc gctcgcactg cagcctgagg ttttttccgt cgagttccag
     4201 gccccgcagg atatcgccgc cgtacacggt tttatggatg agcttgaagt cgagaaactc
     4261 gaccgggaag gcgtcgagag aactcatgat gtactccgga gtcatgacga gaggggcggc
     4321 aatccgtttt ccgccgtatt tcttccccag cggcgccagg aaagagatga accggagatc
     4381 catggaatgg aggacgacga cggagttgat atcggacagc ttttcgtcgt agtccgggat
     4441 aacggcgctg cccacgacgt ggaaggagtg gaggttggaa gcatgcccct tcaggatgtc
     4501 ttcgaccatg ggtgaaagct tttccgcaac gatcggtttc agtccggtga agtcgagatg
     4561 gttcatagtg gcgctattat agcacaggag ccgcacctgc acaatgaaac gaagtgtcgc
     4621 gttgcttttc acggggaact gcgatattct agaaatattc acccggcagc aattctcgtg
     4681 gccgccgatt cagcagggcg acgttgatgt cgttcatgcc gtcctcaggt tcatccgccg
     4741 ctccggcaaa gcgtatcgag gggctatgga gagccttcag catatccttc agtcagtgat
     4801 cctggccatc agtatcgcgg gggcggtggt cgtgatatgg ggcatcgccg aggctgttgt
     4861 cgcttttttc ctgatgaaaa tcggccgtac cggtaaagaa gccgttcagg cgagcgaagc
     4921 gatccggctg cggctcggcg cccatctcct gctggggctt gaaatattca tcgccgcaga
     4981 cattatcagc tccgttgcga ctcccacctg ggagcgggtg ggcatcctgg cgtccatcgt
     5041 agcggttcgg actgtgctca gttatttcct gagaatggag atgaagcagg gtgctgagtg
     5101 agaggactcc ttgcgctgga ggcgaccatg gacccttttg aaaagaagat agcaggtaca
     5161 accatccggc tcgtatcagc cgacctcacg gaacgggacg ttgacgccat cgtgaacgct
     5221 gcgaacagcc agctccggca cggcggcggc gtagcgggcg cgatcgtccg gaagggcgga
//
LOCUS       DASSLD010000503         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_545548,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000503 DASSLD010000000
VERSION     DASSLD010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545548"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2140
                     /locus_tag="VFK23_09120"
     CDS             <1..2140
                     /locus_tag="VFK23_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319282.1"
                     /translation="LPDVRLLDIRNAEIAYLAGEEQEVRLTDFNLTVKDFSPKKGGHI
                     SLRSDFSYPSRADAGISAKGKVAALVELEGFYPIPYGQGSIDLTVDSGQYRSGKRTIA
                     LNDFSLVTKLVYDRRTETFTVDSMTGKSRELGAIKVAARAVMTGSMPWSLNLSVSSMD
                     FSTVFGVARPFLNKDLKGWTVKGKGGVETALQGTFADKQQAFSGTISLSFKETGASSP
                     DGSISAQGASGTVVLKMEYSSPERKLAFNLSSVQRPGEQLWKDYYNNFKEQQASVTAD
                     GTVTLTEDVPFSVIAKLDIFQTGEYTVGMRGNRRDWSMKVMAANVSHEKVIEKMLKEY
                     LNGSTSGLKGFSATGFSSLETLIRRSGAATHVSGVYKMTDTAINAPDLQLSVRTISVD
                     LPFDLVHPSSARKKQVSRVPGSIRLTGIQREKLSLGDVHIPVVIAQNKLEVPEPVVIP
                     FYSGQIEIYDGMADDLLSPDREVRFGLKMKNISLGLLTQKLLDMELPGAINADFGVMS
                     YQRNRLASAGTAVINIFGGEIKAENFFAQDITLPSRKFGGDITFKDINLEQMTGKIAI
                     GRMSGVLRGSLEGFTMEYGEPSGFILEVESVKKKGITQQISSDAINNISILGAGAGSV
                     LSEGITKYFKNFPYSKIGFRCVLKNDKFTVNGTILEGDKEYLVRRGLFRGVDVINQNR
                     NNVISFRDMEERIKRISRPTEAKSGEKLVQ"
BASE COUNT          652 a          595 c          698 g          590 t
ORIGIN      
        1 attgcctgat gtgcggctgc tcgatatccg gaatgctgaa atagcttacc tggcagggga
       61 agaacaggag gtcaggctga ccgattttaa tcttacggtg aaggacttct ccccaaagaa
      121 aggaggccat atctccctcc gatccgactt cagttaccca tcacgcgcgg atgcgggaat
      181 ctcggcaaaa ggcaaagttg ccgccctggt agagctggag ggtttttatc cgataccgta
      241 tggacaaggg tcgatcgatc tgacggtcga ctccgggcag tatcgttccg gcaaaaggac
      301 gattgcgctc aatgactttt cgcttgttac gaagcttgtt tatgacagac gcacggagac
      361 attcaccgtc gactcaatga cgggaaagag cagagagctg ggcgctatca aggttgccgc
      421 cagggctgta atgaccggtt caatgccgtg gagcctgaac ctttccgtat cgtcaatgga
      481 tttctcaacg gtcttcggcg tcgcaaggcc tttcctgaac aaagatctca agggatggac
      541 ggtgaagggc aagggagggg tcgagaccgc gcttcagggg acctttgcgg ataagcagca
      601 agcattcagc gggacgatct cattgtcgtt caaagagacc ggggccagct cgcctgacgg
      661 ttcgatatcc gcgcagggag ccagcggtac tgttgtgctg aaaatggagt attcatcgcc
      721 tgaacggaag cttgcattca acctctcttc tgttcagcgg cctggagaac agctctggaa
      781 ggattattac aacaatttca aagaacagca ggcgtctgtt acggctgacg gaacggttac
      841 cttgaccgag gatgtaccct tttccgttat cgcgaagctt gacatctttc agaccggcga
      901 gtatacggtc ggcatgcgcg gcaatagacg cgactggtca atgaaggtca tggctgcaaa
      961 tgtctcgcac gaaaaggtca ttgaaaagat gctgaaggaa tacctgaacg gctctacgtc
     1021 gggcctgaag ggcttttcgg caaccggctt ctcttcactg gaaaccctga tccggcgaag
     1081 cggagcggca acacacgttt ccggagtata taagatgacc gataccgcca taaacgcgcc
     1141 tgacctgcag ctttccgtcc gcactatttc cgtggatctg cccttcgatc ttgtgcatcc
     1201 gtcttcagca aggaaaaagc aggtctcacg tgtgccgggg agtatccgtc ttacaggcat
     1261 ccagagggaa aagctctccc tgggagatgt tcatatccct gtggtcattg cccagaataa
     1321 gctggaggtg cctgagcccg ttgtgatccc attctattca gggcagatcg agatctatga
     1381 tggaatggcg gatgacctcc tctctcctga tagggaggtc agattcggtc ttaagatgaa
     1441 gaacataagc ctgggccttc tgacacagaa gctgctggac atggaattgc ccggcgccat
     1501 caatgccgat ttcggggtga tgagctatca gagaaaccgg ttggcgtccg caggaacagc
     1561 ggtgatcaat atctttggcg gagagatcaa ggccgagaac tttttcgccc aggacatcac
     1621 gctgccgtcc aggaaattcg gcggcgatat aacattcaag gatatcaatc ttgaacagat
     1681 gaccggaaag atcgccatcg gccggatgag cggggttctc cggggttcgc tggagggttt
     1741 tacgatggaa tacggcgaac cttcgggttt tatcctggag gttgaatcag tcaaaaaaaa
     1801 gggcatcact cagcaaatca gctcggacgc gattaataac atatcgatac tcggcgcagg
     1861 agcaggaagc gttttgagcg aagggattac gaaatacttc aagaatttcc cctatagcaa
     1921 gatcgggttc cgatgcgtgt taaaaaacga caagttcacg gtcaatggca ctatactaga
     1981 aggagacaag gagtatctgg tgcggagggg cttgtttcgg ggtgttgatg taataaacca
     2041 gaaccggaat aatgttatca gtttccggga catggaggag cgtataaagc gcatatcgcg
     2101 gccgaccgaa gcaaagtccg gtgaaaagct tgtacaataa caaataaggt tcttctgact
     2161 tttctgtgcg tcattaaact gtctctgtgt ttattaatgc taaataagat acttttttct
     2221 ttgtcacctt tcttgcgtgc ccaagaaagg tgacccaaag aagggcaccc ggcgaaaatt
     2281 ttttacggca tgcttggtcg tcctcggtac atttcagaaa cttgcccttc gggcttcgga
     2341 catctgaaat gcttaaccct cggactcggt cgcctggcta aattttcgca tgggaaccat
     2401 gcaataaacg ttgatttaaa tcttcaaacc cggaagatcc acaaataaag gcaataaagt
     2461 cgagcatcac cttaaggagg aaaacatgaa gaagaaattg aatttgacgg tcgcgctggc
     2521 ggtggcagtg ctcgt
//
LOCUS       DASSLD010000504         4912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_441170,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000504 DASSLD010000000
VERSION     DASSLD010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441170"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="VFK23_09125"
     CDS             <1..951
                     /locus_tag="VFK23_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HET7319283.1"
                     /translation="LSTRWALEQGSSVRDPGSGKSAIGLFGIQHHYAHIGSVMAEYGI
                     LHKVLGIALDGTGYGTDGNLWGGEFLVAGIEGFERAGHFDYVPLPGGEAAIREPWKTA
                     VSFIIKAGGDDAWDLLNRAGFIRRYGKETLERLMIVSRSRDFSPLSSGAGRLFDAVSA
                     LLGICDLNTFEGESAMALESFTQEGIDGEYRVEFRQENGYTVTDFSPAIIGIIDDLGN
                     ASAAVISTKFHNTVAAVVRTMTGNIAERTGLRDVALSGGTFQNRYLLKRVLEYLAGDG
                     MNVMVNSAVPCNDACISLGQAYLVRERLKKCGIRNSECGV"
     gene            953..2011
                     /gene="hypD"
                     /locus_tag="VFK23_09130"
     CDS             953..2011
                     /gene="hypD"
                     /locus_tag="VFK23_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015145154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HET7319284.1"
                     /translation="MKQMLEELHTLTKLVDRPLKLMEVCGTHTVEIFRHGIRDVIPNS
                     ITLLSGPGCPVCVTSVRDVDAAIAIAQASGVTMTTFGDMMRVPGGKESLLEARSKGAD
                     VRVLYSPLDAVALAQKEPGREIVFFGTGFETTSPLIAGSIARAEQAGIKNISVYAAFK
                     LVPPALKALLDSPDVRVDGFILPGHVSTIIGKRPYEFVATEYKIPAVITGFEAKEIVE
                     GILMIVRQIAEKRAMVEIQYRNVVREEGNPRAVELLNRYFEPSDAYWRGIGVIPGSGL
                     KLKEQYSAFDAQKKFNPPLSDGVEPELCSCGDILRGVKIPTECPLFGTACTPDAPVGP
                     CMVSTEGSCAAYYKYGKI"
     gene            2108..3103
                     /gene="hypE"
                     /locus_tag="VFK23_09135"
     CDS             2108..3103
                     /gene="hypE"
                     /locus_tag="VFK23_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HET7319285.1"
                     /translation="MDRILLGHGSGGRLMHELIRRHFSPAFGIEGAGDSAVVEMPSRR
                     LAYTTDSYVVTPLFFPGGNIGELAVNGTVNDLAVSGAEPLYLTAGFVIEEGFLLADLE
                     KIVASMSSAAVKAGVRIVAGDTKVVNKGKADGIFINTSGIGALPEGREISAVRIRTGD
                     KIIISGELGSHGIAVMAERNGLTFEPPVLSDTRALNGLVRAMFGVTDAIRFMRDPTRG
                     GLATTLKEAALESGLCMHVREQSLPVPSGVRGACGLLGLDPLYVANEGILIAVVDSAA
                     AEPLVAAMRKQEHGRHAAVIGEVSDSPAGIVLLETAIGGRRIIDMLQGEQLPRIC"
     gene            3120..4436
                     /locus_tag="VFK23_09140"
     CDS             3120..4436
                     /locus_tag="VFK23_09140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET7319286.1"
                     /translation="MQPDIRKQVIALALPVVLSSLLQRSVGIVDIFLVGGLGASSIAA
                     VGIAQIMVFVVMSASWGINIGVTVLVSQLWGADRKSDAARAAYQAMLLAAGAAAVISF
                     LGLTYGGGVGSLLGAQAVVRDILFDYTRIIFAFILFTIAINVLAGIMHGTGDTKTPLY
                     ATLIVNVLHVAVAYPLIYGHYGLPRLGVKGAAIAVAISECMGAVFLLVRSLQRGYIKI
                     SREFEAKYTIMTIKLGYPIFVDRLLQNAGSLAFAKVILLYGTAVYAAHQVGLAIEAFS
                     FMPGYGIAVAAATMVGQNLGAGKPEHARISAYEANRFAVILMAAMGLIFFFFPYGLLR
                     AFTSDPEVIAYGVLYMKIVAFAQIPLAVTMVVGGSLRGAGDTGFIMFATIAGMWFVRI
                     PVAAMLAVWFSAEVRYVWSVMIADWVVRMALLLWRYRKESWGKLEI"
     gene            4494..4712
                     /locus_tag="VFK23_09145"
     CDS             4494..4712
                     /locus_tag="VFK23_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319287.1"
                     /translation="MQDIQSMLGKEVEVIANGVRYTGVLIEVSDVEVHIKGPMQWIAL
                     PVSAVSEVKLKTGAAPRPDRFNDWGTGQ"
     gene            complement(4715..>4912)
                     /locus_tag="VFK23_09150"
     CDS             complement(4715..>4912)
                     /locus_tag="VFK23_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7319288.1"
                     /translation="DKFRTLDIEGVSTSSLRQALDEFEREYIRRSLVDNKFSKEAAAG
                     KLGIDLATLYRKLKKLRIETA"
BASE COUNT          990 a         1385 c         1491 g         1046 t
ORIGIN      
        1 ctttcgacac gatgggcgct ggaacaaggg tccagtgtcc gggatccggg gtccggcaaa
       61 tctgcaatag ggctattcgg catacagcac cattatgccc atatcgggtc cgtgatggcc
      121 gaatacggga tccttcacaa ggtcttggga attgccctgg acggcaccgg ctatggtact
      181 gacgggaacc tctggggagg agagtttctc gttgccggaa tcgaaggatt cgagcgcgcg
      241 ggacacttcg actatgtacc gcttcccggc ggcgaggcgg cgatccgcga accctggaaa
      301 accgcggtga gttttatcat caaggcgggc ggcgatgatg cgtgggatct gctcaaccgt
      361 gccggattca ttcgaagata cggcaaggag accctcgaaa ggctcatgat tgtctcgcgc
      421 tcccgcgatt tttccccgct ctcgtcgggg gccggtaggc tcttcgatgc cgtgtcagcg
      481 cttctcggca tctgcgatct gaataccttc gaaggagaat cggccatggc gctggaatcc
      541 tttacgcagg aggggatcga cggagagtat cgggttgagt tcaggcagga aaacggttat
      601 actgtcacgg acttttctcc cgcaatcatc ggcatcatcg acgacctcgg gaatgcatcg
      661 gcggcggtta tctcgacgaa atttcataac accgtagcag ctgtcgtgcg aacgatgacg
      721 gggaacatcg ccgaacggac cgggctcagg gacgtggccc tgagcggagg cacgttccag
      781 aaccggtatc tgttgaaaag agtcctggaa tatctcgccg gggacggcat gaacgtcatg
      841 gtgaacagcg ccgtgccctg taacgacgca tgcatatccc tggggcaggc gtatctggtc
      901 agggagagac tcaagaaatg cggaattcgg aattcggaat gcggagtata agatgaagca
      961 gatgctcgaa gaactccaca cgcttacgaa gctcgtcgac aggccgctca agctcatgga
     1021 agtctgcggc acccataccg tcgagatttt caggcacggc atccgtgacg tgatcccgaa
     1081 cagcatcacg cttctctccg gacccggctg cccggtctgt gtgacatcgg tccgtgacgt
     1141 cgatgcagcc atcgcgatag cgcaggcgtc cggcgttacc atgacaacct tcggcgatat
     1201 gatgcgcgtt ccgggtggaa aggaatccct gctggaggcg agatcgaagg gggccgacgt
     1261 acgggtgctc tattccccgc tcgatgccgt tgctctggcg caaaaggaac ccgggaggga
     1321 gatcgtcttt ttcggtacgg gtttcgaaac cacgtctccg ctcatcgccg gcagcattgc
     1381 ccgcgccgag caggccggca taaagaacat ttccgtgtac gcagcattca aactcgtacc
     1441 acccgcgctc aaggctctcc ttgattctcc cgatgtgcgg gttgacggct ttattctccc
     1501 cggacacgtc agtacgatca tcggcaaaag accgtatgaa tttgttgcga cggagtataa
     1561 aatacctgcc gtgattacgg ggttcgaggc gaaggagatc gtcgagggta tcctcatgat
     1621 cgtgaggcag atcgcggaga agagggcgat ggtcgagata cagtaccgaa atgtcgtccg
     1681 ggaagagggg aatccccgcg ccgttgagct gcttaatagg tatttcgaac cttccgacgc
     1741 ctactggcgt gggatcggag tcattcccgg aagtggcctc aaactgaagg aacaatattc
     1801 cgcatttgac gcccagaaga aattcaatcc tccgctttcg gatggagtcg agcctgaact
     1861 ctgctcctgc ggcgacatcc tgcgtggagt aaagatcccg accgagtgcc ctctcttcgg
     1921 cacggcctgc acgccggacg ccccggtcgg gccctgcatg gtgagcaccg agggaagctg
     1981 cgcggcgtat tacaagtacg gaaaaatctg atcatcaacc gcaaaaagca cagaaggcgc
     2041 aagagttacg attcaaaata tgtgcattcc gtgccttatg tggataattc tctaaattaa
     2101 ggtgtttatg gataggatac tgttaggtca cggcagcggc ggcaggctca tgcacgagct
     2161 gatccgcagg cacttcagcc ccgccttcgg aatagagggc gccggcgatt ccgccgtcgt
     2221 cgaaatgccg tcccggaggc tggcctatac gaccgattcc tacgtggtga cgccgctgtt
     2281 ctttcccggc ggtaatatcg gcgaactggc cgtgaacggg acggtgaatg accttgccgt
     2341 ctcgggcgcc gagccgctct atctcactgc gggcttcgtg attgaagaag gcttcctcct
     2401 ggccgatctt gagaagatcg tcgcctccat gtcctctgct gcagtcaagg ccggcgtcag
     2461 gatcgtcgca ggcgacacca aagtagtgaa caaaggaaag gcggacggga tcttcatcaa
     2521 tacctcgggc ataggcgcac tccccgaggg aagagagatc tcggccgtga gaatcagaac
     2581 aggcgacaag atcattatca gcggcgaact cggcagccac ggcattgccg tcatggccga
     2641 acgaaacggc ctgaccttcg agccgccggt tctcagcgat acgcgcgctt tgaacggtct
     2701 ggtgcgcgcc atgttcggcg tgaccgatgc aatcaggttc atgcgcgacc cgacccgggg
     2761 aggcctcgcg acgacgctca aggaagcggc ccttgaatcg ggcctctgca tgcacgttcg
     2821 cgagcagtcc ctcccggtgc cttcaggcgt ccggggcgcc tgcggcctct tgggcctcga
     2881 tcctctctat gttgcgaacg aaggcatttt gatcgcggtc gtcgacagtg cagccgccga
     2941 accgcttgtt gcagccatga ggaaacagga gcacgggcgg catgcggccg tcatcggaga
     3001 ggtttcggat tctcccgccg gcatagttct gctcgaaacc gctatcgggg gccgacgtat
     3061 aatcgacatg ctccaggggg agcagctccc gaggatctgc tgatttccca tcacttgtca
     3121 tgcagccaga catccgcaaa caggttatcg ccctcgctct tcccgtcgtt ctttccagcc
     3181 ttctccagcg gtccgtcggc atagtggaca tcttcctcgt gggcggcctc ggcgcttcat
     3241 ccattgctgc cgtcggtatc gcgcagatta tggtgttcgt cgtcatgagc gcctcctggg
     3301 ggatcaacat cggcgtgacc gtcctggttt cccagctctg gggagcggat aggaagagcg
     3361 acgcggcaag ggccgcgtat caggcgatgc ttctcgcggc aggagccgct gccgtcatct
     3421 cgtttctcgg cctgacctac ggaggcggcg tcggctccct tctcggagcc caggccgtgg
     3481 tgcgtgatat cctcttcgat tacacaagga tcatcttcgc cttcattctt ttcaccatcg
     3541 cgatcaatgt tctcgcaggg atcatgcatg ggacgggtga tacaaaaaca ccgctctatg
     3601 ccacgctcat cgtgaatgtt ctgcatgtcg cggtcgcata tcccctcatt tatggccact
     3661 acggccttcc ccgactcggc gtcaaagggg ccgccatcgc cgtcgcgatt tccgaatgca
     3721 tgggggccgt ctttctgctc gtacgctccc tgcagcgggg ctacattaag atcagcaggg
     3781 agttcgaggc gaaatacacg atcatgacga tcaagctcgg ataccccatt ttcgtcgacc
     3841 ggcttcttca gaacgcggga tcgctggcgt tcgcgaaagt catcctgctg tacggcacgg
     3901 ccgtgtacgc ggcccaccag gtggggctgg caatcgaggc tttttccttc atgccgggat
     3961 acggcatcgc cgtggccgcg gcaacgatgg tcggccagaa cctcggggcc gggaagccgg
     4021 agcacgcgag gatttccgcc tacgaggcaa accgttttgc ggtcattctc atggcagcca
     4081 tgggactcat cttttttttc tttccctacg gccttctgcg ggcgtttacg agcgatcccg
     4141 aagtgattgc atacggtgtc ctctacatga aaatcgtcgc gtttgctcag attcccctgg
     4201 cagtcacgat ggtcgtcggg gggtcgctcc ggggggcggg agacaccggt tttatcatgt
     4261 tcgcaaccat cgcgggcatg tggtttgttc gcattccggt tgcggcgatg cttgccgtat
     4321 ggttttccgc ggaggtacgc tacgtctggt cggtgatgat tgccgactgg gttgtcagga
     4381 tggccctgct gttatggcgt tatcggaagg agagctgggg gaaactggaa atatgataaa
     4441 acagtagtca ggagtcagga aacaggcgtt acgagaacgc agagggaggg ataatgcaag
     4501 acattcaatc gatgctcggg aaggaagtgg aggttattgc gaacggagta cgttataccg
     4561 gtgtcctgat cgaggtctcg gacgttgaag tgcacataaa aggacccatg cagtggatag
     4621 cgcttcccgt gtcggcggtg agcgaggtaa aactcaagac cggcgcggct ccgcgtcccg
     4681 accgcttcaa tgactggggg acgggacagt agcgtcacgc cgtctcgatc ctcagctttt
     4741 tcagcttccg gtacagggtg gcaagatcga tccccagctt tcccgccgcc gcttccttgc
     4801 tgaacttgtt gtccaccagg ctgcgccgga tatactcccg ctcgaattca tcgagcgcct
     4861 ggcggagcga tgacgtcgag acgccctcga tgtctagggt tctgaacttg tc
//
LOCUS       DASSLD010000505         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1476483, whole genome shotgun sequence.
ACCESSION   DASSLD010000505 DASSLD010000000
VERSION     DASSLD010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476483"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1845)
                     /gene="ade"
                     /locus_tag="VFK23_09155"
     CDS             complement(136..1845)
                     /gene="ade"
                     /locus_tag="VFK23_09155"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HET7319289.1"
                     /translation="MINDLRERIAVARGHRKADLVIRGGRIVNVFSGAIERRDVAVHR
                     GKVVGLGPYDGKTVIDASSKIILPGFIDSHVHLESSLLVPAEFVKAALPRGVTGVVAD
                     PHEIANVLGREGIRYFLRATGGLPLDFYFMLPSCVPASRLETSGARLSSASLATFLRH
                     ERVLGLAEMMNYPGVLAADRDVLRKLARFGGRPMDGHAPGLSGRDLMAYVGAGISSDH
                     ECITAKEAREKAGLGMTVYIREGSAAKDLEALLPAVNPSNARSFCLCTDDLQAGDLLL
                     GSIDQMIRKAVGLGLEPVTAVRMATLNPALHFDLRGKGAVAPGYDADLVVIDNFDRLT
                     VEMVFKKGRLVARGGRVRVPVRHIDHPSTKNTVNIPEFDVSRVRLKARGDRARVIELI
                     PRQIETRQAVLPVRARAGLVISDRSRDILKLLVVERHRSSGNIGIGLVKGFGLSSGAL
                     ASTIAHDSHNIIAVGVDDADIHQAVVEIGRMHGGMVVVDRGKVLARLPLPVAGLMSEK
                     PAATVAKKINEVRQAARRLGTRIEEPFSALSFLALPVIPALKLTDRGLVDVNRFTFVD
                     LFV"
     gene            complement(1799..>2073)
                     /locus_tag="VFK23_09160"
     CDS             complement(1799..>2073)
                     /locus_tag="VFK23_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319290.1"
                     /translation="ASLYNFINLLYLWQERDNLFLIVRYPTPIFLNEIGRKKAQDAQN
                     AGQHFYPFVPLLRIFAAIIMSSVFYLRRRPRHDQRPQRKNRCSERA"
BASE COUNT          419 a          616 c          617 g          421 t
ORIGIN      
        1 ccatcctata aatacacttg gacgcagatg gacgcagatt tgcacagata aagccagacg
       61 attcgtctct tcatttatta tttattttaa tccgcgttta tccgatttga tctgcgtcca
      121 aaacttcttt taaattcata cgaacaaatc aacgaaagtg aaccggttca cgtccacgag
      181 gcccctgtcc gtaagcttca acgcggggat gacggggagg gccaggaacg acagggcgct
      241 gaagggctcc tcgatcctcg ttcccagacg ccgcgctgcc tgccgtacct cgttgatctt
      301 tttcgcgacg gtagctgccg gtttttccga catgagccct gccaccggga gtgggaggcg
      361 cgccaggacc tttccccggt ctacgacgac catcccaccg tgcatccggc cgatctcgac
      421 gactgcctgg tgaatgtcgg cgtcatccac gcccacggcg atgatgttat gggaatcgtg
      481 ggcaatcgtc gaggccagtg ccccggagga gagtccgaag cccttgacaa gaccgatgcc
      541 gatattcccc gaggaccggt gacgctcgac cacgaggagc tttaaaatgt cccgtgacct
      601 gtcggaaatc acgaggcctg cgcgggcccg gaccggcagg actgcctgcc tggtctcgat
      661 ctggcgcggt atgagttcga tcacccgtgc ccggtctccc cgggccttga gccggacacg
      721 agaaacgtcg aactccggta tgttcacggt gtttttcgtg gacgggtggt cgatgtgcct
      781 cacgggtacg cggaccctcc ccccgcgagc gaccagcctt ccttttttga aaaccatttc
      841 cacggttaac ctgtcgaagt tgtcgattac gaccaggtct gcgtcgtatc ccggcgcgac
      901 cgcgcctttg ccccggagat cgaaatgcag cgccgggttg agcgttgcca tgcggaccgc
      961 ggtaacgggc tccagtccca gccccaccgc tttccggatc atctggtcga ttgagccaag
     1021 gagaaggtct cccgcctgaa ggtcgtccgt gcagaggcag aaggaacggg cgttcgacgg
     1081 gttgactgcg ggcagaagcg cctcgaggtc ctttgcggcc gagccctccc ggatatagac
     1141 cgtcatgccg agtcccgcct tctcgcgggc ttccttcgcc gtaatgcatt cgtggtccga
     1201 tgatattccg gcaccgacat acgccatgag gtccctgccg gaaaggccgg gcgcatggcc
     1261 gtccatgggg cggcctccga accgggcaag cttgcgcagg acgtcgcggt cagctgcgag
     1321 tacccccggg tagttcatca tctcggccag gccgaggacc cgttcatgcc gcagaaaggt
     1381 cgccaagctg gcggaggaaa gacgcgctcc cgaggtctcc agccggctcg cgggaacgca
     1441 ggagggaagc atgaagtaga aatcgagcgg cagcccgccg gttgcgcgaa ggaaatacct
     1501 gatgccttcc ctgcccagga cgttggcaat ctcgtggggg tcggcaacca cgccggtgac
     1561 cccccggggg agcgcggcct tgacgaattc ggcggggacg agcagggagc tttccaggtg
     1621 cacgtggctg tcgatgaaac cgggaaggat gatttttgag gatgcgtcga tgacggtttt
     1681 gccgtcataa ggcccgagtc ctacgacctt gccccggtgg accgccacat cgcgccgttc
     1741 gatcgccccg gaaaatacgt tgacgatccg gccgccccgg atgacgaggt ccgccttcct
     1801 atgccctctc gctacagcga ttctttctct gaggtcgttg atcatggcgc ggcctccggc
     1861 gcagataaaa cactgaagac attattattg ccgcaaagat gcgcaggagt ggcacaaagg
     1921 gatagaaatg ttgacctgca ttttgcgcat cttgcgcctt tttgcggcca atttcattta
     1981 aaaaaatcgg cgtaggatac cggactatca ggaagagatt gtccctttct tgccaaagat
     2041 acaacagatt gataaaattg taaagagagg ctg
//
LOCUS       DASSLD010000506         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1444182, whole genome shotgun sequence.
ACCESSION   DASSLD010000506 DASSLD010000000
VERSION     DASSLD010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444182"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFK23_09165"
     CDS             complement(<1..579)
                     /locus_tag="VFK23_09165"
                     /inference="COORDINATES: protein motif:HMM:NF017019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HET7319291.1"
                     /translation="MTDIIKKNAGKTFSFAYTFNNALSSPYFPSAAYEENGFAVGLQS
                     INLSAGCQKIEQWLERMKAVWLEIDVLFSAEEGYLGIDTSIAPLFAGSGSFIHLITRL
                     GLTLSHAVTTDVFLRIANSIRKDGPRKVGLCGLMFPCLEDFELAREYEEGRFSIERNA
                     FLSLHSGLGIDAYPIGVDEKPDRVAEILRLIQG"
     gene            610..846
                     /locus_tag="VFK23_09170"
     CDS             610..846
                     /locus_tag="VFK23_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319292.1"
                     /translation="MSQVNIEVHTFCVAELRQYGESLIKMHPADTEEDASIALSIQAE
                     HTVKVGGTQSARLNGKSIIRKPVCQVFQPCNGPL"
     gene            complement(1022..1972)
                     /gene="trxB"
                     /locus_tag="VFK23_09175"
     CDS             complement(1022..1972)
                     /gene="trxB"
                     /locus_tag="VFK23_09175"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET7319293.1"
                     /translation="MDEKDTYDIIIVGGGPAGLTAGLYAKRAAMKTILFERGLIGGQI
                     AVSKDVDNYPGMEGITGFDLTEKLIHHARSFDLHVRPQAVSMISTGTDFHTVLLSDGE
                     ELQTVALILALGGSARKLGVPGETKYLGTGVSYCGTCDGFFFRDKTVVVVGGGDTAVE
                     EALYLSRLAKKIYLVHRRNELRAAKMLVNRLMNEPRVNVIWNTVVTEIRGDGLLVDSV
                     LLENVETGEKRTLQTDGVFIFIGYAPNNLLIPPAIRMNERGYVVTDEKCETNVPGIFA
                     AGDLRQKFANQIVVATADGCIAALAAARYVETRRAMALLS"
     gene            2130..>2590
                     /locus_tag="VFK23_09180"
     CDS             2130..>2590
                     /locus_tag="VFK23_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7319294.1"
                     /translation="MTIYLDNNSTTLPAPEVRDAMLAYLRGKYGNASSIHAAGREARK
                     VLEESRETIAHILGAARPECICFTSSGTEADNMAIRGVAGALRHKGNHIVTSAVEHPA
                     VLNTCKFLEEGGARVTYVRPDETGMILPEAVEDALRDDTILISIMHANNETG"
BASE COUNT          583 a          771 c          673 g          563 t
ORIGIN      
        1 gccctgaatc agacgcagga tttccgctac tctatcgggc ttctcatcaa ccccgatcgg
       61 atacgcgtcg attccaagcc ccgagtggag cgacaggaag gcgttccgct ctatggaaaa
      121 gcggccttct tcgtactccc gggccagttc aaaatcttcg aggcagggaa acatgagccc
      181 gcacaaaccc accttccggg ggccatcctt cctgatagag ttcgcaattc tgaggaacac
      241 atcggttgtc actgcatggc tcagggtaag accgagccgt gtgatcaggt ggataaaact
      301 gccgctgcct gcaaagagcg gcgcaatgga cgtgtcaatg ccgagatacc cttcttcggc
      361 tgagaagaga acgtcgattt ctaaccacac ggccttcatt ctctcaagcc attgctcgat
      421 tttttgacac ccggccgaga ggtttatcga ctgcagtccg acagcaaaac cgttttcttc
      481 gtaggcggcg gaggggaaat aagggctgga aagcgcgtta ttgaaggtgt aggcaaagct
      541 gaaggtcttc ccggcgttct tcttgataat gtccgtcaat aagcgtgtat gcctcaccgt
      601 gacgggttca tgagtcaggt caacattgaa gtccacacgt tttgtgttgc agaattgcgg
      661 caatatggcg aaagcctcat caaaatgcac cctgccgaca ctgaggaaga tgcttccatc
      721 gccctctcta tccaggctga acacaccgtc aaggtcgggg gaacacaatc tgcgcgtttg
      781 aacggtaaat ccattatcag aaagcctgtt tgccaggtgt tccagccttg caacggcccc
      841 ctttgaaggc ctgtcgataa aaaggcagac ggttctgacg attttgtgct gctccatctg
      901 cggcgggtct ccttgagaat agttcgcggg atgccggatg agggacgctc cccctcaggc
      961 tacggcaggc tgttcggatt gttacggctc catcaaggta aaaaagggga aattctgcag
     1021 atcatgacaa cagcgccatg gccctccgcg tctccacgta ccgcgccgcg gcaagcgccg
     1081 ctatgcatcc gtctgccgtc gcaaccacga tctggttggc gaacttctgg cgcaggtccc
     1141 ctgctgcgaa gatccccgga acgttcgtct cacacttctc atccgtgacg acgtagcccc
     1201 gttcgttcat tcggattgcc ggcgggatca gcagattatt cggagcatac ccgataaaga
     1261 tgaatacacc gtccgtctgc agcgtccgct tttcccctgt ttcgacgttc tcgagtaaca
     1321 cggagtcgac cagcaaaccg tctccccgga tctccgtcac cacggtattc cagataacgt
     1381 tgacacgcgg ttcattcata agacgattga ccagcatttt cgcggcccgc agctcgtttc
     1441 ttctatgaac gaggtatatt ttctttgcca gcctcgacag atacagcgcc tcttccacgg
     1501 cggtgtctcc ccctccgact accacaacgg tcttgtccct gaagaaaaaa ccgtcgcagg
     1561 tgccacaata agacacgccg gtccccaggt attttgtttc accggggacc ccgagctttc
     1621 gggctgaccc ccccaaggca aggatcaggg ccacggtctg gagctcctcg ccgtctgaaa
     1681 gcagcaccgt gtgaaaatcc gttcctgtcg atatcatgga taccgcctgg ggcctgacgt
     1741 gcaggtcgaa ggacctggca tgatggatca gtttctcggt cagatcgaac ccggttatcc
     1801 cctccatacc gggatagtta tccacgtctt tcgatacggc gatctggccg ccgatcaatc
     1861 cccgttcaaa aagaatcgtt ttcattgcgg ccctttttgc atagagcccc gcggtgagtc
     1921 ccgcaggccc gcctcccacg atgattatgt cataggtgtc cttctcatcc ataccgttcc
     1981 tccccggaag catcaccagg cacccgcaac catgcggatg cggacacgtt ccttcttcct
     2041 tatctaccta ctagtatatc gcgttttcgc tgagctataa taaaaaggaa gtgttcgaaa
     2101 cagccaatca tgaaggaaat gatataccga tgaccatcta tctggacaac aactctacga
     2161 ctttgcccgc acccgaggta cgggacgcca tgctggccta tctcaggggg aaatacggca
     2221 acgcctcctc gatccatgcc gctggacggg aggccagaaa ggtcttggag gaatcaaggg
     2281 aaaccatcgc gcatatcctg ggggccgcgc gtccggaatg catctgcttc accagctcgg
     2341 gcacggaagc cgacaacatg gcgatccgcg gggtcgccgg ggccctccgc cacaagggga
     2401 accacattgt cacgtctgcc gtggagcatc ccgccgtcct caacacctgc aaatttctgg
     2461 aagagggagg agcccgtgtg acttacgtga ggcccgatga aaccgggatg atcctgccgg
     2521 aagccgtcga agatgcctta cgggacgaca ccatcctgat ttccatcatg cacgccaaca
     2581 acgaaacggg
//
LOCUS       DASSLD010000507         3509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_962911,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000507 DASSLD010000000
VERSION     DASSLD010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962911"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..777)
                     /locus_tag="VFK23_09185"
     CDS             complement(13..777)
                     /locus_tag="VFK23_09185"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HET7319295.1"
                     /translation="MTLSVYRTLQSLLLPPAGLLLLMALGFVIMIICRTFGRLLVASG
                     FILLYLVSIDPVGDALIRPLESAYHPLNAAPKDRKAGAIVVLGGGVRDLAWAGLDPEP
                     SEMSLERVVAAVRLYRSAHLPLVVVGGSGDPSKPDISEAESMAHTASGLGVPRRDLIV
                     IENARNTLESAAAAWEKLKGERIILVTSAFHMRRAVGMFRRKGFQVIPAPCGYRSEER
                     TLTIRSFTPRADCLHSSHAALAEYLSLAWYGTSGDL"
     gene            complement(975..>3509)
                     /locus_tag="VFK23_09190"
     CDS             complement(975..>3509)
                     /locus_tag="VFK23_09190"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET7319296.1"
                     /translation="SLAGLPEQEAAERLRREGYNELPSSKKRSTFAIAVGVMREPMFL
                     LLVACGAIYLVLGDVREALMLLGFVFVVIGITLYQERKTERALEALRDLSSPRALVIR
                     GGKQMRIPGREVARGDCVVLNEGDRVPADAAVLSCLNLSVDESLLTGESVPVRKTGCA
                     EIPPMARPGGEDLPFVYSGTLVVKGQGIATVLATGTGTEIGKIGKALQSVELEKTPLQ
                     TETATIVRNLAFVGALLCAIVIVVFGMTRGDWLNGFLAGVTLAMALLPEEFPVVLTIF
                     LALGAWRISQKRVLTRRVPAVETLGAATVLCVDKTGTITQNRMSVARMYARGEFFTVD
                     SRSSKSIPEQFHELAEFSILASQKDPFDPMEKAFRELGNERLARTEHLHDDWVLVREY
                     PLSNSLMALSHVWKSPDGNEYVIAAKGSPEAIADLCHLDEDRRRGLSAAIEGMAKDGL
                     RILGVAKSSFRIADLPPEQHDFSFEFLGLIGLADPVRPGVAEAVTECHAAGIRVVMIT
                     GDYPGTAANIGSQIGLGHNIVSGLELDGMDDDMLRQRIGTVNIFARVVPEQKLRIVNA
                     LKANGEVVAMTGDGVNDAPALKAAHIGIAMGGRGTDVAREASALVLLDDDFSSIVQAV
                     RMGRRIFDNMRKAVSYILAVHVPIAGMSLLPVLFKWPLVLLPVHIVFLELIIDPACSV
                     VFEAEPEEADVMTRPPRRPGAPLFERRTVGISLMQGLIVLAVLLAVFTISLKRGQSGE
                     EARALAFATLIIANLGLILANRSWSRVITDTLRTPNTALWWVAGGALLFLGLVLYVPF
                     LRSLFRFSFLHITDVAICMTAGAFSILWFELLKAVNRRRGIRSGLF"
BASE COUNT          679 a         1115 c         1088 g          627 t
ORIGIN      
        1 cgaatccttc ccttacagat cgccgctcgt cccgtaccat gcaaggctca ggtattcggc
       61 aagtgctgca tgggaggagt gcaggcaatc cgcgcggggg gtgaatgagc ggatcgtaag
      121 cgtccgctcc tcactcctgt atccgcaggg agcggggatc acctgaaagc ccttcctgcg
      181 gaacattcca accgcccgcc tcatgtgaaa cgcggaagta acgagtatga tgcgctctcc
      241 tttgagcttc tcccatgcgg cagctgcgct ttccagggtg ttccgcgcat tctcgattac
      301 gatcaggtcg cggcggggca caccgaggcc cgaagccgtg tgcgccatgg attcggcctc
      361 gctgatgtcc ggcttcgacg ggtcgccgct cccgccgacc acgacgagcg gcagatgcgc
      421 gctccggtag agccgtactg ccgcgaccac cctctccagg gacatttcgg acggttcggg
      481 atcgagtccc gcccaggcga ggtcgcgcac gccgcctccc aggacgacga tggcacccgc
      541 cttcctgtcc ttcggtgcgg cgttaagcgg atggtaggca ctctcgagcg gcctgatcaa
      601 tgcatcgccc accggatcga tactcacgag gtagaggaga ataaaacccg aagccacaag
      661 cagcctgccg aatgtccggc atatgatcat gatcacaaag cccaatgcca tgagaagcag
      721 aagacctgcg ggtggaagca ggagcgactg cagggtcctg tataccgaaa gcgtcatggt
      781 tctcctcttc ccgcctgcgc gccgataatg aacgtgacct ccttatcggc tccggctctt
      841 tacttgactg cactgacaca atattttgaa tcatctcccc gatagcagca ttcgaggacg
      901 gctgaaagaa tgtcattccc gcagagcctg cccctgcaat cagtaagccg gtgtcaaccg
      961 ggaaacaggg tttttcaaaa caaaccgctc ctgattcccc gccgcctgtt caccgccttg
     1021 agcagttcga accacaggat gctgaaggct cctgccgtca tgcagattgc cacgtccgtg
     1081 atgtgcagga acgagaaccg gaataggctg cggagaaaag gaacgtacag gaccagcccc
     1141 aggaacagga gcgctccgcc cgctacccac cagagcgccg tattcggcgt tcgcagcgtg
     1201 tcggtgatga cgcgcgacca ggaacggttg gccaggatga gcccgagatt ggctatgatg
     1261 agcgtggcaa aagcgagcgc ccgcgcctcc tccccgctct ggccgcgttt cagggaaatc
     1321 gtgaacaccg cgagcaggac ggccaggacg atcagtccct gcatgaggct gatccccacg
     1381 gtccgcctct cgaacagcgg cgcgccgggc cggcgcggcg gccgagtcat cacgtccgcc
     1441 tcctccggct cggcttcgaa cacgacggag catgccgggt cgatgatgag ttcaaggaac
     1501 acgatgtgca ccggcaaaag caccaggggc catttgaaga gcacggggag gagggacatg
     1561 cccgctatgg ggacgtgaac ggcgagaatg taggaaacgg cctttctcat gttgtcgaag
     1621 atcctgcggc ccattcgcac cgcctgtacg atggaggaaa aatcgtcatc gagcagcacg
     1681 agggccgagg cctcgcgcgc aacgtccgtt cccctgcctc ccatggcgat gccgatgtgg
     1741 gcggccttga gggccggggc gtcgttcacg ccgtcgccgg tcatggccac gacctcgccg
     1801 ttcgccttga gcgcgttcac gatcctgagc ttctgctcgg gaacgacccg ggcaaaaata
     1861 ttgacggtcc cgatgcgctg ccggagcatg tcatcgtcca tgccgtcgag ttccaggccg
     1921 ctcacgatgt tgtgaccgag gccgatctgg cttcctatgt tcgcagccgt tccgggataa
     1981 tctccggtaa tcatgacgac ccggatgccg gcggcatgac attccgtcac cgcttccgcc
     2041 acgcccggac gcacgggatc ggcaaggccg atgagcccga ggaattcgaa ggagaaatcg
     2101 tgctgttccg ggggcaggtc cgcgatcctg aaagaggact tggccacgcc gaggatgcgc
     2161 agtccgtcct ttgccatgcc ttcaatggcc gctgacagcc cgcggcggcg gtcttcgtcc
     2221 agatggcaga gatcggcgat cgcctcgggc gagcccttcg ctgcgatcac gtactcattc
     2281 ccgtcgggcg acttccagac gtgggacagc gccatgaggc tgttcgaaag cggatattcg
     2341 cgcaccagca cccagtcgtc gtggaggtgc tcggtccggg caaggcgttc gtttcccagt
     2401 tcgcggaatg ctttttccat ggggtcgaag gggtccttct ggctcgccag gatgctgaac
     2461 tccgctaatt cgtggaattg ttcgggaatg ctcttcgaag accggctgtc aacggtgaaa
     2521 aactcgccgc gcgcgtacat cctggcgacc gacatgcggt tctgtgtgat cgtgccggtc
     2581 ttgtcgacgc acagcaccgt ggcggccccc agggtctcga cagccggaac gcgccgggtg
     2641 agcacccgtt tctgcgaaat acgccacgcg ccgagggcga ggaaaatcgt gagcaccacg
     2701 gggaattcct cgggaagaag cgccatcgca agggtgacgc cggccagaaa cccgttcagc
     2761 cagtctcccc gcgtcattcc gaacacgacg atcacgatcg cgcacaggag cgcgccgacg
     2821 aaggcgaggt tcctgacgat cgtcgccgtc tccgtctgga gtggagtttt ttcgagctcg
     2881 acactctgga gcgctttgcc gatctttccg atctcggtac cggtgccggt cgccagcacc
     2941 gtggcgatcc cctgcccctt gaccacaagc gtcccggaat agacaaaggg caggtcctcg
     3001 cccccgggcc gggccatggg gggaatctcg gcgcagccgg tcttgcggac cggcacggat
     3061 tccccggtaa ggagtgattc atccaccgag aggttgagac aggacagaac cgccgcgtca
     3121 gccggaacgc ggtccccctc gttcaggacg acgcaatcgc cgcgcgcgac ctctcgcccc
     3181 ggaatgcgca tctgcttgcc gccgcggatg accagggcgc gcgggctcga aaggtcccgg
     3241 agtgcttcaa gggcccgttc ggtctttcgt tcctgataca atgtgatgcc tatgaccacg
     3301 aagacgaagc cgagcagcat gagcgcttcc ctcacgtcgc cgaggaccag gtaaatggcg
     3361 ccgcaggcaa cgaggagcag gaacatgggc tcgcgcatga cgcccacggc gatggcgaag
     3421 gtgcttcgct tcttcgagga cggcagctcg ttgtatccct cgcgccggag ccgttcggct
     3481 gcttcctgtt ccggaagacc tgccaggga
//
LOCUS       DASSLD010000508         3305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_498051,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000508 DASSLD010000000
VERSION     DASSLD010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498051"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..3023
                     /locus_tag="VFK23_09195"
     CDS             336..3023
                     /locus_tag="VFK23_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET7319297.1"
                     /translation="MTVKDALVELNAGSNGLSAEEARERLVRYGPNELVEKKRRTAVR
                     MFLGQFTDFMILVLIAAAAVSGVIGEAADSVAIIVIVLLNAALGFSQEYRAEKAMSAL
                     KKMASAHAVALRGGGQRTIPAAELVPGDVVLLDAGNIVSADMRILEAARLKVQEAALT
                     GESLPVEKTAVAIPEEPLPLGDRRNMLYKGTFVTYGRVVALVTATGMNTELGRIAAML
                     QEEDEGKTPLQKRLAVFGKKLAYAVLAICAVIFAAGLLRGEKPVEMFLVAVSLAVAAI
                     PEALPAVVTIALALGARKMVKRNALVRKLPAVETLGSVTYICTDKTGTLTQNKMTVEE
                     VWVDGKTVRSSELGVVSKDALRTPNSALRTLLTALALSNDARLDSSGGTIGDPTEAAL
                     FSFAHVRGFEKATLEKEFPRSAELPFDSERKLMTTIHKISPSSIVQSLPSNAFIGGPS
                     SLLLGGTYVSFTKGAFDALIDRADFVLTAAGPKPMDRGEVLALHDRMAADGLRVIGIA
                     MHAWERVPSEISPVETGLTLLGLAGMLDPPREEAKEAVARCKAAGITPVMITGDHPLT
                     AQVIAGRVGILESDDPRAVMTGVELDRLSLDEYEKRVETIRVYARVAPEQKLKIVKAL
                     QDRGHYVAMTGDGVNDAPALKAADIGVAMGITGTDVAKEASSMILLDDNFATIVKAVK
                     EGRRIYDNIRKFVKYLLSTNSGEIWTLFLAPFFGLPVPLQPIHILWINLLTDGLPALA
                     LSLEPEEGDIMGRPPRRPAESIFAHGLGVHAVWVGLLMAAVTLSLQAWAIHIGDAHWQ
                     TMVFTVLCFLQLGHVMAIRSERVSLFTQGVMTNPTLFASVLGMIVLQLATIYIPPLNP
                     LFRTQPLTAVEFLAVFALSSIVFLAVELEKLIRRRQKTA"
BASE COUNT          662 a         1029 c          986 g          628 t
ORIGIN      
        1 gcaggataaa aaacatcagt gctatgacgc gcaagttggc ctgcatgaat ggtctgcctt
       61 tgcgggaaac ggttacagga caaaaagaaa cttatttaag tatatcacgc agggcggacg
      121 atgacctcgc ggctttacaa gcaaacgaaa ccatgctacc tttttggtac taaatctttt
      181 ttgacattcc gccccggccg gaatcatgcg cggcaggccc cggtaggaat ccattttgtg
      241 agaccaggga tgcctgctct ccgattaaat cattcgagga caagtttcgc agcaggcatg
      301 acagaacaag tgagggtcct ttgcaatttc accagatgac cgtcaaagac gcgcttgtcg
      361 agttgaatgc gggaagcaac ggcctcagcg ctgaagaagc gcgggagcgg ctggtgcgct
      421 acggcccgaa cgagctcgtc gagaaaaaga ggaggacggc ggtacgaatg ttcctgggac
      481 agttcaccga cttcatgatc ctggtcttga ttgcggccgc agcggtctcc ggggtgatcg
      541 gcgaagcggc cgattccgtc gcgatcatcg tcatcgtcct gttgaacgct gcgctcggtt
      601 tcagccagga gtaccgcgct gaaaaggcca tgtcggccct taaaaaaatg gcctccgccc
      661 atgcggtcgc gctgcggggc ggcggccagc ggaccattcc cgctgccgaa ctggtccccg
      721 gagacgtcgt gctgctcgac gccggcaata tcgtgtccgc ggacatgcgc atcctcgagg
      781 ccgcgcgcct gaaggtccag gaggcggccc tgaccgggga atcgctgccc gtggaaaaga
      841 cggcggtcgc catcccggaa gagccccttc ccctggggga ccggaggaac atgctgtata
      901 aaggcacgtt cgtaacctac ggccgcgtcg tcgctctggt gacggccaca ggcatgaaca
      961 cggagctcgg caggatcgcg gccatgcttc aggaggagga cgagggcaag acgccgctcc
     1021 agaagcggct cgcggtattc ggcaagaagc tggcctatgc cgtgctcgcg atctgcgccg
     1081 tcatcttcgc cgcgggcctg cttcggggag agaagccggt cgagatgttc ctggtcgcgg
     1141 tcagcctcgc cgtggcggcc atacccgagg ccctgcctgc ggtcgtgacg attgcccttg
     1201 cgctcggggc gcgcaagatg gtaaagagaa acgcccttgt cagaaagctc cccgcagtcg
     1261 agacgctcgg ctccgtcacc tacatctgca ccgacaagac cgggactttg acgcagaaca
     1321 aaatgacggt ggaggaagtg tgggtggacg ggaagacagt tcggagttcg gagttgggag
     1381 tggtgagtaa agacgctctc cgaactccga actcagcact ccgaactctt cttacggctc
     1441 tcgccctgtc caacgacgcg aggcttgact cctcgggggg caccatcgga gatcccacgg
     1501 aggcagcgct tttttccttc gcgcatgtcc ggggcttcga gaaggcaacc ctggaaaaag
     1561 agtttccgcg ttcagccgag ctgccgttcg attccgagcg caagctcatg acgacgatcc
     1621 ataaaatctc cccttcgtcc atcgtccaaa gcctgccctc gaatgctttt atcgggggtc
     1681 cctcctccct cctcctcggc ggcacgtacg tgtctttcac caaaggcgcg tttgatgcgc
     1741 tgatcgaccg cgcggacttc gtcctcaccg cggcgggtcc gaaaccgatg gaccgcggcg
     1801 aggtcctcgc gctccatgac cgcatggcgg ctgacgggct ccgggtcatt ggcatcgcca
     1861 tgcacgcctg ggaacgcgtc ccctccgaga tttccccggt tgaaacgggc ctcacgctcc
     1921 tcggcctggc gggcatgctg gaccccccgc gggaggaggc gaaagaggcc gtggcacgat
     1981 gcaaggccgc ggggatcacg ccggtaatga tcaccggcga ccaccccctg actgcgcagg
     2041 tcatcgcggg gcgggtcggc atcctcgaat cggacgaccc ccgggcggtg atgaccggcg
     2101 tcgagctcga ccggctttcc ctcgacgagt atgaaaagcg ggtcgaaacc atccgtgtct
     2161 acgcccgggt cgctccggag cagaaactca aaatcgtgaa ggccctgcag gaccgcggtc
     2221 attacgttgc catgaccggc gacggcgtga acgacgcgcc cgcgctcaag gcggcggaca
     2281 tcggcgtcgc catggggatc accggtacag acgtggccaa ggaagcgtcg tccatgatcc
     2341 tcctggacga caacttcgcg accatcgtga aggccgtgaa ggaaggccgc aggatctacg
     2401 acaacatccg gaagttcgtc aaatacctgc tctccaccaa ctccggcgag atctggaccc
     2461 tcttcctcgc accgttcttc gggcttccgg tcccgcttca gccgatccac atcctctgga
     2521 tcaaccttct gaccgacggc ctgcccgccc tggccctctc cctggaaccg gaagaaggcg
     2581 acatcatggg ccggccgccg cgccgccccg cggagagcat attcgcccac ggtctcggcg
     2641 tccacgccgt ctgggtcggc ctgctcatgg cagcggtcac cctttcgctc caggcatggg
     2701 cgatccatat cggggacgcc cactggcaga ccatggtctt taccgtcctc tgtttcctcc
     2761 agctcggcca tgtcatggcc atccggtcgg aacgggtgtc gctgttcacc cagggcgtca
     2821 tgacgaaccc gacgctgttc gcctccgtgc tgggcatgat cgtccttcag ctcgcgacga
     2881 tctacatccc gccgctcaac cccctcttca ggacccagcc gctcaccgcc gtggagttcc
     2941 tggccgtctt cgccctgtct tccatcgtct ttcttgccgt tgagctggaa aagctgatca
     3001 ggcgccgcca aaaaacagct tgacttttgg ataccggccg tatcataatt cctcacgatt
     3061 tgataaattc gattgctgaa agggggcttc attccgcctg cccgggcgga agaaacgaaa
     3121 gcttattcta taatgatgtg atcgagctgc aagacatacc aactcgctca tgggggaggt
     3181 gatgcagcct ggcgtatccg ccatttacct gaagtgtttt caccatggaa cagagtatcg
     3241 aagttgcgtg atgaaatact taccttggga aagggggacc acatcagcat ggaagaaaaa
     3301 ccaaa
//
LOCUS       DASSLD010000509         3871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1309118, whole genome shotgun sequence.
ACCESSION   DASSLD010000509 DASSLD010000000
VERSION     DASSLD010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309118"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VFK23_09200"
     CDS             <1..628
                     /locus_tag="VFK23_09200"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET7319298.1"
                     /translation="NPNCSTIAMVMVLRPIHDRAKIRRVVVTTFQSVSGTGKEAMDEL
                     ANQTVALLNFRDVETKVYPHQIAFNCIPHIDSFLDDGYTREELKMLQETRKILEDDSI
                     RVTATTVRVPVFRCHSEAVNIELETKITANEVRAVLAAAPGVIVYDDPKKNLYPLAVD
                     VAGKDETYVGRIREDESVPNGINLWIVSDNLRKGAALNAVQIAELLTK"
     gene            638..1477
                     /locus_tag="VFK23_09205"
     CDS             638..1477
                     /locus_tag="VFK23_09205"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HET7319299.1"
                     /translation="MYKLISKIFDKLERGLSPRDKTIIVTLMLTISVLGIVLAVRYYQ
                     YIQKNPEFCNGCHLMEEAYKAWKLSGHRNMVCQDCHQLNWIEQNRLLVKFVFTTDRKT
                     PEPHGNETPWKACTKCHWDDAAQGSLTVNKSTGHARHVFMEKLSCKDCHSKKVHSFIP
                     EKDACLRCHKDWKIHGVGMEDISCLRCHTFSPKKLEAFIPDREHCLSCHRKSSKTSFP
                     DRVPMARLNCYECHKPHARIKPTDDDCLRCHTREVLALKRAHESTRHCKDCHVPHRWT
                     SNR"
     gene            complement(1498..1740)
                     /locus_tag="VFK23_09210"
     CDS             complement(1498..1740)
                     /locus_tag="VFK23_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7319300.1"
                     /translation="MTLAEKLNKLRLRNKLSMSQVSRMSQSASDSRGRITQGYISRLE
                     SGKETNPSLMKLVTLCRIYKIEPNELFVRNRRKKTK"
     gene            1919..2107
                     /locus_tag="VFK23_09215"
     CDS             1919..2107
                     /locus_tag="VFK23_09215"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7319301.1"
                     /translation="MYKIEILERKRLEKGLSYTEIADKLGMHKVTVSRTLKGVTLKPR
                     TVKLLADYLGVQMETIVQ"
     gene            2248..3042
                     /locus_tag="VFK23_09220"
     CDS             2248..3042
                     /locus_tag="VFK23_09220"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7319302.1"
                     /translation="MDEYLTVASNAARRAGNILRENLHGIREISYKGDINLVTEMDMR
                     SERAVVETILASFPGHGIIAEEETCIRNESGYRWVIDPLDGTTNYAHGYPCFSVSVAL
                     EHEGEVVAGVVYDPMRDELFTARKGKGAALNGRPIRVTAVDTLIKSLLATGFPYDRKE
                     SEKNNMDYFHDLLMASQEVRRDGSAALDLCYVACGRFDGFWELKLKPWDVAAGSLIVR
                     EAGGIVSDFSGRRFSIHDGEILATNGSIQRKMIEVLQKAGDKRSGL"
     gene            complement(3031..>3871)
                     /locus_tag="VFK23_09225"
     CDS             complement(3031..>3871)
                     /locus_tag="VFK23_09225"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HET7319303.1"
                     /translation="GIVLAGLGYIAVLPDYVGYGDSTNELHPYVHAATLASATINMNR
                     AARTFLADPSINRTTNGQLFLTGYSEGGYATLAAQRLMEQSLASEFPITASEPGAGPY
                     DISGTTVSILSSPTLSQPAFAGFLLKAYDSIYNTPSQLSHYFTTTYVSVVDTHFDGSF
                     SRSQITADLGGADVATNALFNQAFLTSYLGAGETALKNHIKENDVYDWAPKVPTRLFH
                     SPEDDTVPFANTTTAEAKMKANGSTTVTVVDICPAVPATHTNCAKPFALDMIAFFNSL
                     LKP"
BASE COUNT          897 a          996 c         1143 g          835 t
ORIGIN      
        1 aaaccccaat tgctccacca ttgccatggt catggtttta aggccgattc acgacagggc
       61 gaagatcagg cgtgtcgtgg tgacgacgtt tcagtcggta tcgggcacgg gaaaagaggc
      121 catggacgaa ctcgccaatc agaccgtggc gctcctgaat ttcagggacg tggaaacaaa
      181 ggtctatcct caccagatcg cgttcaactg catcccgcac atcgactcct tcctcgatga
      241 cgggtatacg agggaggagc tgaagatgct ccaagagacg aggaagatcc tggaagacga
      301 ctcgatacgc gtgacggcga ctacggtgcg cgttccggtg ttccggtgcc attcagaggc
      361 ggtcaatatc gaactggaaa caaagattac ggccaacgag gtgagggcgg tcctcgcggc
      421 ggctccgggg gtcatcgtat acgacgaccc gaagaagaat ctctacccgc tggcagttga
      481 tgtcgccgga aaggacgaaa cttacgtggg caggatacgg gaggacgagt cggtcccgaa
      541 cgggatcaac ctctggatcg tatcggacaa cctgcggaag ggtgcggcgc tcaatgcggt
      601 gcagatagcg gaactgctga caaagtagtc aggagtcatg tacaaactca tctccaaaat
      661 attcgataag ctcgaacggg gcctgagccc cagggacaag acgatcatcg ttaccctgat
      721 gctcacgatc tcggtcctcg ggatcgtcct ggccgtgcgg tattaccagt atatccagaa
      781 gaatccggag ttctgcaacg gctgtcacct gatggaggag gcatacaagg cgtggaagct
      841 ttccggccac cggaacatgg tctgtcagga ctgccaccag ctcaactgga tagaacaaaa
      901 caggctgctc gtgaagtttg tcttcacaac cgaccgcaag acgcctgaac cgcatggaaa
      961 tgaaacgccg tggaaggcct gtacgaagtg ccattgggac gacgccgcgc aggggtcgtt
     1021 gaccgtgaat aaatccacgg gacatgcccg gcatgtgttc atggaaaagc tttcctgcaa
     1081 agactgccac agcaaaaagg ttcactcctt cataccggaa aaggatgcat gccttcgctg
     1141 ccataaggat tggaagattc acggcgtcgg catggaggac atatcctgcc tccggtgcca
     1201 tacattttcg ccgaaaaaac tggaggcctt cattcccgac agggagcatt gcctctcgtg
     1261 ccatcgaaaa tcatcaaaaa cgtcatttcc cgacagggtg cccatggcgc ggctcaactg
     1321 ctatgagtgc cacaagcctc atgcgcgcat caagccgact gatgacgatt gtctccgctg
     1381 tcacacgcgc gaggtgctgg cgctcaaacg ggcccacgag tcgacccggc actgcaagga
     1441 ttgccatgtg ccgcaccgct ggacctcgaa ccgctgacgc gcggcagccc tctcaagtta
     1501 tttcgttttt ttcctgcgat ttcgtacgaa gagttcgttt ggctcgatct tgtagatcct
     1561 gcagagcgtg acgagcttca tgaggctggg attggtctct ttacccgatt cgagacgcga
     1621 gatgtatcct tgcgtaatcc tgccgcggct atcagaggcc gattgcgaca tgcgcgacac
     1681 ctgactcatc gacagtttgt ttcggagacg cagtttgttc agtttttcgg cgagtgtcat
     1741 acgggactac ttataccctg atttagcaat attgtcaaca ggaagttgcc aaaaaattat
     1801 tatctttatt gttgaacatt gcaaagattt gtactatact cataactgta agtttagatc
     1861 atatctatct acggtctttg gtcgcggagt atggttaatc ttcttgtgga gggacgccat
     1921 gtataagatt gaaattcttg aaagaaagcg tctggaaaag ggcttgagct ataccgagat
     1981 tgcagacaag cttggaatgc ataaagttac agtctcgaga acgctcaagg gggtcactct
     2041 gaagccgcgg accgtgaagc tccttgcgga ttacctcggt gttcagatgg agacaatcgt
     2101 ccagtagaag tcgttgagaa ttcgtttatt ttatgcgctg tgcagaggag aagagcaagg
     2161 cgaagaggcc ttgccttttt tttgtaactt caaagcaaca ctctgcaaga tcatgtcgta
     2221 gacagcggga atgatggagg atggacgatg gatgaatatt taaccgttgc aagcaatgca
     2281 gctcgcaggg cgggaaatat cctgcgagaa aacctgcacg gcatccggga aatttcttat
     2341 aaaggcgaca tcaacctcgt gaccgagatg gacatgcgtt cggaacgggc cgtggtcgag
     2401 acgatccttg cttcttttcc cggtcacggc atcattgccg aggaggagac gtgcattcgg
     2461 aatgagtccg gctatcgctg ggtcatcgac ccccttgacg gcaccaccaa ttacgcccat
     2521 gggtatccct gtttctccgt gtccgtcgcg ctcgagcatg agggcgaggt agtcgcgggc
     2581 gtggtctatg atcccatgcg agacgagctg ttcacggcga ggaagggcaa gggggctgca
     2641 ctgaacgggc ggccgatccg ggtcaccgcc gtcgacaccc tgatcaagag ccttctcgca
     2701 acgggattcc cctatgatcg caaggaaagc gagaaaaaca acatggacta tttccatgat
     2761 ctgctcatgg cgtcgcagga ggtgcgccgg gacgggtccg ccgcgctcga tctctgttat
     2821 gtcgcgtgtg gccgcttcga cggcttctgg gaactcaagt tgaagccctg ggacgtggcg
     2881 gcgggaagcc tgatcgtgag agaggccggg gggatagtct ccgatttctc cggccggcgc
     2941 ttcagcatcc atgacgggga gatcctcgcc acgaacggaa gcattcaacg caagatgatc
     3001 gaggtcctgc aaaaggccgg agacaagcgg tcagggcttt aacaaagaat tgaaaaaggc
     3061 gatcatgtca agggcgaaag gctttgcgca attggtatgg gtcgccggca cagcagggca
     3121 gatgtcaacc acggtcaccg tggtcgatcc atttgccttc atcttcgcct cggcggtcgt
     3181 ggtgttggcg aagggaacgg tgtcgtcctc cgggctgtgg aacaggcggg tggggacttt
     3241 cggtgcccag tcataaacgt cgttttcctt gatgtgattt ttgagggcgg tctcgcccgc
     3301 gcccaggtag cttgtaagaa acgcctgatt gaacagtgca ttcgttgcaa catcggcccc
     3361 gccgagatcg gcagtgatct ggcttctgct gaagcttccg tcaaaatgcg tatccacaac
     3421 gctcacgtag gtcgtagtga aatagtgact gagctggctt ggcgtgttgt agatggagtc
     3481 ataggccttg agtaaaaatc cggcgaaggc cggctgcgac agggtgggtg acgacaggat
     3541 gctcacggta gtgcccgata tgtcgtaagg cccggcgccg ggttccgaag cggttatcgg
     3601 aaactccgaa gccaggctct gctccatgag cctctgcgca gcgagcgttg cgtatcctcc
     3661 ctcggaatag ccggtaagaa ataactggcc gttcgtggtc cggttgatcg acgggtccgc
     3721 cagaaacgtg cgcgccgccc ggttcatatt gatcgtggcc gaagcaagcg tcgccgcgtg
     3781 tacgtagggg tgcagttcat tcgtcgaatc gccgtagccg acgtaatccg gcagcactgc
     3841 gatgtatccc agccctgcga ggacgatgcc c
//
LOCUS       DASSLD010000510         2913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1515710, whole genome shotgun sequence.
ACCESSION   DASSLD010000510 DASSLD010000000
VERSION     DASSLD010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515710"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..292)
                     /locus_tag="VFK23_09230"
     CDS             complement(29..292)
                     /locus_tag="VFK23_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017307611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET7319304.1"
                     /translation="MSRKRIVEVFTAGCPLCDETVNLVKKLSCPSCDVRVYNLKENGM
                     DKAKQYGVNSVPTVVVDGKVAECCKRGKPSEADLRAAGIGTPW"
     gene            complement(547..>2913)
                     /locus_tag="VFK23_09235"
     CDS             complement(547..>2913)
                     /locus_tag="VFK23_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7319305.1"
                     /translation="LEGNDKHVSTRGRLCAKGNSGIGHIYDPDRILYPLKRTGPRGSG
                     RWKRVSWDEAIAEVAAKITAIEASGHPNEIMMKWGRNRSGGAAERFMATLGSNTIVNH
                     TSICEASKKIGMEPTWGPDIETPDFENTKYILNFGSNILEAGYFMNPYSQRIMDGVVD
                     GKAKLVTFDVRLSNTAAKSDEWFPIFPGTDGIVALAMANVIMQEGLADRNFINTWTNY
                     PADKLEEHLKQFTPEMAEKYSGVPAKDIRRIAIEFGSVRPATTYTYRGPCKHLYGSYN
                     ERCTMLLSIITGNIDVKGGYCLPRGMDYNQPQPTPPKAKEPSYLSTPPDYPLAHYKVS
                     QLTPFWIKEGKQKIGVYFNYMSCEAFTNPAASYWREVLLDEKLIPFSVTFSNQMNETT
                     VLMDLVLPDCSYLERHDPEGMPSSLWPWLGIRQPVVKPLGEAIEFREILKMIIQKVDP
                     DGKKGMRKYWEFKDGEDYLRRQFENIPGLKEAGGYDFLKKNGVWPVYGTLDPATGKIM
                     GKDGKLIKPEYGLYMKEDPKGKVTVGGKKRKGFGTPDGKINIYVAEYEKYGFSPLPTF
                     KPHPWHWEKNGASKLRDDQMILSTFKWNVHVQSRTANLKWMNEIVHKNPAWLNPDTAK
                     KFGIKDGDLIRITTPVGYMVTYAKVTEGIHPRVVAVSNTLGMKFGRVATVDKDGPMPM
                     WGGVEEPDLKNIWWKAEGVNPNDVIPVSADPIGGSQGWYDTVASVAPAKPGDKFGDVS
                     FDNKKHFEFFKETLRYAYTGDKHREMHPEVEVKELPPRQLGGEKIEKL"
BASE COUNT          495 a          885 c          774 g          759 t
ORIGIN      
        1 atacgaaggg cgcccatgca tccagaatct accatggcgt gccgatgccc gctgccctga
       61 gatcagcctc gctcggtttg ccccgcttgc agcactcggc gaccttcccg tccacgacca
      121 cggtaggcac ggagttcacg ccgtactgct tcgctttatc catcccgttt tctttcagat
      181 tataaaccct tacgtcgcag ctcgggcagg aaagcttttt cacgaggttt acggtctcat
      241 cgcaaagcgg gcatcctgcc gtaaatactt ccacaattct cttccgtgac atatcactca
      301 cctcctttat ggtttatatc ttaaaccttc cagccggatg gagagtcaag gtcttttttc
      361 cggtctcctc gatcagcgga cagaatgaag tcgccgaatg ctttctcccg ttaactattg
      421 tctataattg tctttccgta ccgtttttgc ccctatccgc gatgttttct gcccaacctt
      481 tgctcttacc gccaaagtgg gcggtcagaa ttggccgccc acttgaactt acacccctgg
      541 taccggtcag agcttttcaa tcttctctcc cccaagttgt cgtggcggca gttcttttac
      601 ctctacctcc ggatgcatct cccggtgttt gtcgcccgtg tacgcatacc gaagggtttc
      661 cttgaagaac tcaaagtgct ttttgttgtc gaatgaaaca tcgccgaact tgtccccggg
      721 ctttgccgga gcgacggatg cgacggtgtc gtaccagccc tgcgaaccgc cgatcgggtc
      781 tgcggagact gggattacgt cgttcggatt gaccccttcg gccttccacc agatgttctt
      841 caaatccggc tcttcaacgc cgccccacat cggcattggc ccgtccttgt caaccgttgc
      901 gaccctgccg aatttcatcc ccagcgtgtt tgaaacagcc acgacgcggg ggtggatccc
      961 ctcagtgacc ttcgcgtagg tcaccatata ccccacggga gtcgtgatcc tgatcaggtc
     1021 tccgtccttg ataccgaact ttttggcggt atcggggttg agccaggcgg gattcttatg
     1081 cacgatctca ttcatccatt tcaggttggc agtcctgctc tgcacatgga cgttccactt
     1141 gaaagtgctc aggatcatct gatcgtccct gagttttgaa gcgccgttct tctcccagtg
     1201 ccacgggtga ggtttgaacg tcggcagcgg gctgaacccg tatttttcgt actctgcgac
     1261 gtagatgttg atcttcccgt ccggcgtgcc gaaacccttg cgcttcttgc cgcccacggt
     1321 taccttcccc ttcgggtctt ccttcatgta aagaccgtac tccggtttga tcagtttgcc
     1381 gtccttgccc atgatctttc cggtcgcggg gtcaagagtg ccgtatacgg gccagacgcc
     1441 gttcttcttg agaaagtcgt agccgcccgc ttccttgagc ccgggaatat tctcgaactg
     1501 cctccggaga tagtcctcgc cgtccttgaa ctcccagtac ttccgcatgc ccttctttcc
     1561 gtcggggtcc actttctgga tgatcatctt gaggatttcc ctgaactcga tcgcctcgcc
     1621 cagcggcttc acgaccggct gcctgatgcc gagccacggc cacaggctcg aaggcatgcc
     1681 ctcgggatcg tgccgttcga ggtagctgca atccggcagg acgaggtcca tgagcacggt
     1741 ggtctcgttc atctggttcg agaaggtgac gctgaagggg atgagctttt cgtcgagcag
     1801 cacctcccgc cagtagcttg cggcggggtt cgtgaacgcc tcgcaggaca tgtagttgaa
     1861 atacactcct attttctgct tcccctcttt gatccagaag ggtgtcaact gactcacctt
     1921 gtagtgcgcc aggggataat caggcggcgt ggaaaggtag ctcggctcct tggccttggg
     1981 cggcgtgggc tggggctggt tataatccat gcctctcggt aggcagtatc cgccctttac
     2041 gtcgatgttt cccgtgatga tggagaggag catcgtgcac cgctcgttat aggagccgta
     2101 gaggtgcttg cacggtcccc ggtaagtgta ggttgtcgcg ggcctgacgc ttccgaactc
     2161 gatagcgatc cgtctgatgt cctttgccgg taccccggaa tatttttccg ccatctcggg
     2221 ggtgaactgc ttcaaatgct cttcgagctt gtcagccgga tagttcgtcc aggtgtttat
     2281 gaagttcctg tccgcgagcc cttcctgcat gatgacgttc gccatggcga gggctacgat
     2341 gccgtccgtg cccgggaaga tcgggaacca ctcgtcgctc ttggctgctg tattggagag
     2401 ccgcacgtcg aaggtgacaa gcttcgcctt gccgtccaca acgccgtcca tgatccgctg
     2461 cgagtaggga ttcatgaagt acccggcctc gaggatgttg ctcccgaagt ttaggatgta
     2521 cttcgtgttc tcgaagtccg gggtctcgat gtccggcccc caggtgggct ccatgccgat
     2581 cttcttggag gcttcgcaga tggaggtgtg gttcacgatc gtattggagc cgagggtcgc
     2641 catgaaccgt tctgccgcgc ccccggagcg gttcctgccc cacttcatca tgatctcatt
     2701 gggatgcccg ctggcttcga tcgcagtgat ctttgctgcc acctctgcga tcgcctcgtc
     2761 ccaggagaca cgcttccacc tcccgctccc ccgcggccct gtccttttca ggggatatag
     2821 tatcctgtcc gggtcgtaga tatgcccgat gcccgaattg cctttcgcgc agagcctgcc
     2881 gcgcgtagac acatgcttgt cgtttccttc aag
//
LOCUS       DASSLD010000511         3475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1459731, whole genome shotgun sequence.
ACCESSION   DASSLD010000511 DASSLD010000000
VERSION     DASSLD010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459731"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1322)
                     /locus_tag="VFK23_09240"
     CDS             complement(156..1322)
                     /locus_tag="VFK23_09240"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET7319306.1"
                     /translation="MKRISILGSTGSIGRSTLAIVEKFPGRFRVVALAAGNNIDLLEK
                     QVRRFRPTVVSVSGEAQAGLLKKRLDGLGVGIFAGVGGMVRVAAADEADITVSAIVGT
                     AGLVPTMAAIRAGKQVALANKEVLVTAGELVMAESRSRGAAILPVDSEHSAIFQCLQA
                     GANNEVRRLILTASGGPFRTLSRKDLEEVTPGQALKHPNWSMGKKITIDSATLMNKGL
                     EVIEARWLFGVPPEKIRVLVHPQSIVHSMVEYQDGAVVAQLGMPDMKGPIAYALTYPE
                     RLAGVSPDLDLARVGALTFEEPDTGRFPCLAYAFDALRAGGTMPAVLSAANEVAVGCF
                     LEGKIGYGDIARVIRSTMDAHTPVPVGTVEDALAADLWARGEAERIIEHGKVGK"
     gene            complement(1406..2206)
                     /locus_tag="VFK23_09245"
     CDS             complement(1406..2206)
                     /locus_tag="VFK23_09245"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET7319307.1"
                     /translation="MLHKRVITGLLFLPVFYLVAWKLPPPFFTALVLAAVITGLYEFY
                     RMARARGGRPQVAVGMLLGAFTVVEFYHPLLPQLGFFFPVAVLLLVALVGRLFSPRPV
                     EGALEDAAATLLGVLYVALLFAFQVVIRTGADGREWLVFLYFIIWASDIGAYSVGIPF
                     GRHRLYEKVSPKKSIEGLAGALIASAGMALLCRVWFMPPVGAGEAVVLALVLAAVGTI
                     GDLVESLFKRSAGIKDSGGIIPGHGGILDRMDSMLFAAPVLYYYLRIR"
     gene            complement(2407..3156)
                     /locus_tag="VFK23_09250"
     CDS             complement(2407..3156)
                     /locus_tag="VFK23_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009733615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET7319308.1"
                     /translation="MNKKSPSHEEQLDKAGLPRHVAIIMDGNGRWALRKGLPRIAGHK
                     RGVDTVRRVTEECRRIGIEVLTLYAFSDENWGRPKEEVGFLMDLLGTFLKSEIATMKS
                     NDIRFRAIGRIERLPSSARKWIDKAASETAANTGMVLNLALSYGGRGEILEAVRRLSA
                     EGVPPQQLTDEVFSSHLDTAGLPDPDLIIRTSGEKRISNFLLWQAAYAELYFTDTLWP
                     DFAEKDLHAALVEYQGRQRRFGLTQEQLARK"
     gene            complement(3235..>3475)
                     /locus_tag="VFK23_09255"
     CDS             complement(3235..>3475)
                     /locus_tag="VFK23_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319309.1"
                     /translation="PKNSGGTILRGHTQAEFVSLGWTGARLEQKWAIKDVGGAVVDFQ
                     VLRQEATGARILALVRSSGGLFSRDTIKVVSYPAK"
BASE COUNT          696 a         1140 c          988 g          651 t
ORIGIN      
        1 cttacgacgt ggtatcatca catgttgtcg gtatatcagt atagatgtaa tgacgctgag
       61 aaggtgagcg ggtaggaagg tgggaaaaaa ccgaaatcac aaacagtaat catcttgcct
      121 tctcaccctc ctaccttccc accttccagc tttaattact ttcctacctt cccgtgttct
      181 atgattctct ccgcttcccc cctcgcccag aggtctgccg cgagggcatc ctcgacggtc
      241 ccgaccggga ccggcgtatg ggcgtccatc gtggagcgga tcacgcgtgc aatgtcaccg
      301 tacccgattt tcccctccag aaaacatccg accgccacct cattcgccgc cgagagaacc
      361 gcaggcatgg tgccgcccgc cctgagcgca tcaaaggcat aggccaggca gggaaaccgg
      421 cccgtatcgg gttcctcgaa ggtgagcgcg ccgactcgcg caagatcaag gtcgggagac
      481 acgcccgcaa gcctttcggg ataggtgagg gcgtacgcta tcgggccctt catgtcgggc
      541 atgccgagct gggccacgac cgcgccgtcc tgatactcca ccatggaatg aacgatgctc
      601 tgcgggtgca cgagaacccg tattttttcg ggggggacgc cgaaaagcca gcgcgcctcg
      661 atcacctcaa gacccttgtt catgagcgtg gccgaatcga tcgtgatctt ctttcccatg
      721 ctccaattcg ggtgtttcaa cgcctggccc ggcgtcacct cttcgaggtc cttccgggac
      781 aaggtccgga acgggcctcc cgatgcggtc aggatcagcc gcctcacctc gttgtttgcg
      841 ccggcctgca ggcactgaaa gatggcgctg tgctcgctgt ccacgggcag gatggcggcc
      901 ccccggctgc gggattcggc catgacgagt tcaccggccg ttaccagcac ctccttgttc
      961 gcgagcgcaa cctgcttgcc cgcccggatc gccgccatgg tcggcacgag cccggccgtg
     1021 cccacgatgg ccgagaccgt gatgtccgct tcatccgccg ccgcgacccg gaccatgccc
     1081 ccaactccgg caaagatgcc gacgcccagg ccgtccagcc gcttcttcag gagcccggcc
     1141 tgagcctctc cggaaacgga cacgacggtg ggacggaacc ggcgcacctg tttctcgagg
     1201 aggtcgatat tattgcccgc ggcgagcgcg acgaccctga accgtccggg aaacttctcg
     1261 acaatcgcaa gcgtcgaccg gccgatggac cccgtagagc cgagtatgga gatacgtttc
     1321 ataaaatcag aagacagaag tcagaaaaca gaagtcggaa gacaactctg tcctctgtct
     1381 tccggcctcc ggcatctgca cttcctcacc ttatcctcaa atagtaatac aagaccggcg
     1441 ctgcgaagag catgctgtcc atcctgtcca ggatgccgcc gtggcccggg atgatccccc
     1501 ccgaatcctt gatcccggcc gaacgtttga ataacgattc cacgaggtct ccaatggttc
     1561 cgaccgcggc gagcacgagt gcaagtacga ccgcctcgcc cgcgccgacg ggcggcatga
     1621 accagacccg gcacaagagg gccatcccgg ccgacgcgat cagcgcgccg gcgagtccct
     1681 cgatgctctt cttcgggctt accttttcgt acagacggtg cctgccgaac gggatgccga
     1741 cggagtaagc tccgatgtcc gatgcccaaa tgatgaagta cagaaacacc agccactccc
     1801 tgccgtccgc gccggtccgg atcacgactt ggaaggcgaa cagcaaggcc acgtagagaa
     1861 cgcccaggag cgtcgccgcg gcgtcctcaa gcgccccttc caccggccgg ggggagaaca
     1921 gccgtccgac aagggccacg agcagcagca ccgcgaccgg gaaaaaaaat cccagttggg
     1981 gaagcagcgg atgataaaac tcgaccacgg tgaaagcgcc gagcagcatg cccacggcga
     2041 cctgcggacg gcccccccga gccctcgcca tgcggtaaaa ttcgtacaac cccgtaatga
     2101 cggccgccag gaccaacgcc gtaaaaaacg gcggaggcag cttccaggcg acgagataga
     2161 atacggggag aaagagaagg cctgtgatca ctctcttatg cagcatgcat ggactccgcg
     2221 gatgaatctc gccacaaacc tgtatttgat ccctttgccg tcattctgaa ctgagcgaag
     2281 cgaagtgaag aatccggccg gtgaaggctc tgcgaaaatt cccggatatt cgacgaacaa
     2341 ggttcttcgc tgcgctcagg atgacgattt cgatgacatt cggcttctca acgccccgac
     2401 atgccgtcac ttccgggcca gctgctcctg cgtcaacccg aacctgcgct gccgcccctg
     2461 gtactcgacg agcgccgcgt gcaggtcctt ttccgcgaag tcgggccaga gcgtatcggt
     2521 aaaataaagc tcggcgtacg ccgcctgcca caggagaaag ttgctgatcc gcttctcgcc
     2581 gctcgtgcgg atgatcaggt ctgggtcggg aaggcccgcg gtgtccaggt gcgaggaaaa
     2641 gacctcgtcg gtaagctgtt gcggcgggac cccctcggcg cttaaccgcc tgaccgcctc
     2701 cagaatttct ccccttccgc cgtaggacaa cgcgaggttc agcaccatgc cggtattggc
     2761 cgcggtttcg gaggctgcct tatcgatcca cttgcgggcc gaggacggca gacgctcgat
     2821 cctgccgatg gcgcggaacc tgatatcgtt tgacttcatg gtcgcgattt ccgacttgag
     2881 aaaggtcccc agcaggtcca tgagaaaccc gacctcttcc ttgggccttc cccagttctc
     2941 gtcggaaaag gcatacagcg tcaggacttc gatgccgatc ctccggcact cttccgtgac
     3001 gcggcgaacg gtgtcgacgc cccgcttatg accggcaatg cgcggcaggc ccttgcgcag
     3061 ggcccaccgg ccgtttccgt ccatgataat ggcaacgtgg cgcggaaggc ccgccttgtc
     3121 gagctgttct tcgtgggaag ggcttttttt attcatagtc gcggttcata gttctattcc
     3181 gcattccgaa atccgcatca gcgtggcccg tttcatcggc catccgcatt tgactcactt
     3241 cgccggatag ctcacaacct tgatggtgtc gcgggagaac aatccgccag aggacctcac
     3301 cagcgccagg attcgggcgc cggtggcttc ctgccgcagg acctgaaaat cgactacggc
     3361 gccccccacg tctttgatgg cccatttctg ctcgagccgc gctcctgtcc agccgaggct
     3421 gacgaattct gcctgcgtat gtcccctcag aatcgtcccg ccgctgttct tcggg
//
LOCUS       DASSLD010000512         3983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_9598,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000512 DASSLD010000000
VERSION     DASSLD010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9598"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..462)
                     /locus_tag="VFK23_09260"
     CDS             complement(67..462)
                     /locus_tag="VFK23_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET7319310.1"
                     /translation="MLKKSSGTPEQAEITAFLGKGTEFKGVLSFEGTIRVDGKVEGEI
                     LSKDTLIAGDGAFLQGEISVGTLISSGKIKGNVTAGQKVHLLAPAAVQGTIKTPKLII
                     DEGVTFDGKSEMAGEGKALDQNAAPIAGK"
     gene            complement(557..1402)
                     /locus_tag="VFK23_09265"
     CDS             complement(557..1402)
                     /locus_tag="VFK23_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET7319311.1"
                     /translation="MQKQALGKGLGALIPDLSTLDDREKKALGITEIELDKIIPNDYQ
                     PRKVFDDGKLRELASSIKEQGVIQPVIVHKAGSGYQLIAGERRWRASRLAGLKTIPAL
                     VKEAGKRELLEMALIENIQREDLNPLEAAEAYKRLQDEFKLTQEDLARRVGKERSTVT
                     NFLRLLHLPKEVKQELASGSLSMGHAKALLSLERGRDQVQAAALIAKKGLSVREAEAL
                     ATRLKNPPKEKRARLSHELKAVEEKLTKALGTKVSITAKSNGGRIVIDYYSPEDLDRI
                     LEKIS"
     gene            complement(1380..2156)
                     /locus_tag="VFK23_09270"
     CDS             complement(1380..2156)
                     /locus_tag="VFK23_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007476797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7319312.1"
                     /translation="MGRVIAIANQKGGVGKTTTTVNIAASLAAAEKKVLLIDSDPQGN
                     SSSGMGIDRSQITGSTYDLYTGKKQLAEIKQSTHFPWLDVVPAKIDLVGVEIELIQMI
                     ARERVLKKAIANFKDNYDYILIDCPPSLGLLTVNALTAADGVIIPVQCEYYALEGLTA
                     LTNTIKLIKQELNPDLALEGVLLTMYDTRNNLAEQVAQEVRKYFGSKVYATVIHRNVA
                     LSEAPSHGKPALLYDIRSRGAQNYLELAKEVIGNAKAGAR"
     gene            complement(2482..3549)
                     /locus_tag="VFK23_09275"
     CDS             complement(2482..3549)
                     /locus_tag="VFK23_09275"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HET7319313.1"
                     /translation="MAEGTLKIPTEWREIAQEIVVQGGPVLVMGAPGSGKSTFCLYLA
                     GIFCSQHRKVAWIDADPGQPIIGPPAAISLTPFTEASELLRRRNPMIMSFIGNTSPVG
                     RLLEMIAGLQKLVTLAHALGPDLVLINTCGLVGGGAARELKFHEIDMLSPRYVIAIQK
                     GTEVEHLLAPHSHRAGLLIRRLPVSPDARISTGEARRAAREQRFKEYFRGADFQEIAL
                     HDVGVHGPGLSTGERLGFRDINRLSKILQAIVIHAELSFDRLFLIVEGEFAESELFTA
                     KEQYQVREVMVMKRSEFDHLIVGLNDEQNLCLGLGIVRTIDLKELSLRVITPLRDVSG
                     IRHVSLGSLRVSPAGGELGQV"
     gene            complement(3575..3868)
                     /locus_tag="VFK23_09280"
     CDS             complement(3575..3868)
                     /locus_tag="VFK23_09280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HET7319314.1"
                     /translation="MGLAGAEVIDKRIEEIENSSWSLYDVVTARAFATMGAALAAGAP
                     FLRTGGLMVLSRGPEETISEKEFRNTGVHVEKKIELMLPHSDYKRAIWVFKKT"
     gene            complement(3837..3956)
                     /locus_tag="VFK23_09285"
     CDS             complement(3837..3956)
                     /locus_tag="VFK23_09285"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319315.1"
                     /translation="MKIIKPDISVTLVESVKKKGLLPQAYHQDIGTRRGRSHR"
BASE COUNT          773 a         1191 c         1105 g          914 t
ORIGIN      
        1 caacacgaag tgcggttaaa aaaaatgggc acgggatcct tcgtttctcc gtgtcggtga
       61 tttcccttac ttgcctgcaa tcggcgcggc attctggtcc agggccttgc cctcgccggc
      121 catttcgctt ttgccgtcga aggtgactcc ttcatcgatg atcaacttcg gcgtcttgat
      181 cgtgccctgg acggccgcgg gggccaggag gtgaaccttc tggcctgcgg tgacgttacc
      241 cttgatcttt ccgctcgaga tgagcgttcc gacggagatc tctccctgga ggaaggcgcc
      301 gtcacccgcg atgagcgtgt ctttcgatag gatctctcct tccaccttgc cgtctacccg
      361 gatagttcct tcgaagctca agaccccctt gaactccgtg ccttttccca ggaatgcggt
      421 gatctcggcc tgctccggcg tgcccgaact cttcttcagc attgattcct ccttaaacga
      481 tggatgatac atgattcatg atgcatgata gaagaatcca ccgtagccat cctgcatctt
      541 gcatctgtat cgtgtctcag cttatcttct ccaaaatccg gtccaggtcc tcgggcgaat
      601 aataatcgat cacaatcctt ccaccattcg atttcgcggt gatgctcact ttcgtgccga
      661 gtgccttcgt aagcttttcc tccaccgctt tgagttcgtg gctcagcctt gccctcttct
      721 ctttcggcgg atttttaagc cgtgtagcca acgcctctgc ctctcgcacc gaaagcccct
      781 tcttcgcgat gagcgccgcg gcctgcacct ggtcgcgccc acgttccagc gacaggagcg
      841 ccttggcatg acccatgctg agcgagccgg aagctagttc ctgcttcacc tccttcggca
      901 ggtgcaggag ccgaaggaag tttgtcaccg tcgagcgctc cttgcccacc ctccgggcaa
      961 ggtcctcctg ggtgagcttg aattcgtcct ggagcctttt gtacgcctcg gccgcttcga
     1021 gggggttcag gtcttcgcgc tggatgtttt cgatcagcgc catttcgagg agttcccgct
     1081 tgcccgcctc cttgaccagg gccggtatgg tcttcaggcc ggcaaggcga gatgcgcgcc
     1141 accggcgttc cccggcgatg agctggtagc cgcttcctgc cttgtgcacg atgacgggct
     1201 ggatcacgcc ctgctctttg atcgatgagg ccagttcccg gagctttccg tcgtcgaaaa
     1261 ccttccgggg ctggtagtcg ttcgggatga tcttatcgag ctcgatttcg gtgatgccca
     1321 gggccttctt ttccctgtcg tcgagcgtgg acaggtccgg gatcagcgcg ccgagccctt
     1381 taccgagcgc ctgcttttgc attgccgatg acctcctttg cgagttcaag gtaattctgc
     1441 gcgccgcggg accggatgtc gtataacagc gccggcttgc cgtggctcgg cgcctcggaa
     1501 agcgcgacgt tgcggtggat gacggtcgca tagaccttgc tcccgaaata cttgcgcacc
     1561 tcctgcgcca cctgctcggc caggttgttc ctggtgtcgt acatcgtgag gagcacaccc
     1621 tcgagggcaa ggtccggatt cagctcctgt ttgatcagct tgatggtatt cgtgagcgcc
     1681 gtcagcccct cgagcgcgta gtactcgcac tgcaccggga tgatgactcc gtccgcagcg
     1741 gtcagagcat tgaccgtcag caggccaagg gacggggggc agtcgataag gatgtaatcg
     1801 tagttgtctt tgaagttcgc gatcgccttc ttgagcacgc gctcgcgcgc gatcatctgg
     1861 ataagctcga tctcgacgcc caccaggtcg atctttgccg ggaccacgtc gagccaggga
     1921 aagtgcgtgg actgctttat ctccgccagc tgcttcttgc cggtgtagag gtcataggtg
     1981 ctgcctgtga tctggctccg gtcgatcccc attccgctcg aagaattgcc ttggggatcc
     2041 gagtcgatca agagcacctt cttttccgct gctgcaagcg acgccgcgat gttcacggtc
     2101 gtcgtggtct tgcctacgcc gcctttctga ttggctattg caatgaccct gcccatgccc
     2161 cttgaacccc ctgaagaccg gttcacaacc cgggtaattt accataggat accgataaaa
     2221 ggaaggaaaa attgaaaaag aactgaaagg gaggaaaacc gaaacctgca tgacgcggat
     2281 ggacgcaaat aaatcatggc aaggcctatc ccctatctct gcactccgca agaagccccc
     2341 cggttgttcg ggaaaccgca ccgggcatgt tccacgtgga acatacccgg gagggaataa
     2401 atagccgtga tttcgaggag cggatcgtca cgaaagttgg tcattgcttg aaacatcgag
     2461 atgttccacg tggaacacca cctatacctg tcccagttct ccccccgcag ggcttaccct
     2521 cagagaaccg agggagacat ggcggatgcc ggagacatcc cggagcggcg tgatcacgcg
     2581 aagggagagt tccttcaggt ctatggtgcg cacgatgccg agcccgaggc agaggttctg
     2641 ctcgtcgttc aggccgacga tcaaatggtc gaactccgac cgcttcatga ccataacctc
     2701 ccgcacctga tactgttcct tggccgtaaa gagctctgat tcggcaaact cgccctcaac
     2761 gatgagaaaa agccggtcga aggacagctc ggcatggatc acgatagcct ggaggatctt
     2821 ggaaagcctg ttgatatccc ggaacccgag cctttcaccg gtgctgaggc ccggaccgtg
     2881 tacgcccaca tcatggagcg cgatctcctg aaagtcagca ccgcggaaat actccttgaa
     2941 gcgctgttct cgggccgcac gccgtgcttc tccggtagag attcgcgcat cgggcgagac
     3001 gggcaggcgt cggatcagga gaccggcgcg gtgggaatgg ggcgccaaca agtgttcgac
     3061 ctcggtcccc ttctggatgg cgatcacata ccggggggaa agcatgtcga tctcgtggaa
     3121 tttaagctcc cgtgcagcgc cccctcccac cagtccgcag gtattgatga ggacaaggtc
     3181 gggtccgagc gcatgcgcga gcgtgaccag tttctgaagg cccgcgatca tttccagcag
     3241 gcgtccgacc ggcgaggtgt tgccgatgaa gctcatgatc atgggatttc ttctgcgcag
     3301 cagctcggaa gcctcggtaa agggcgtgag ggagatggcc gccggtggac cgatgatcgg
     3361 ttggccggga tcagcgtcaa tccacgccac cttccggtgc tggctgcaga agatccccgc
     3421 gagatagagg cagaaggtgg acttgcccga acctggcgcg cccatcacga gaacgggccc
     3481 tccctggacc acgatttcct gggcaatttc ccgccattca gtcgggattt ttaatgtgcc
     3541 ttcagccata aaaactcaga acaacatgga ataattacgt ttttttaaac acccagatcg
     3601 cgcgcttgta atccgaatga gggagcatca gttcgatctt tttttccacg tggacgccag
     3661 tattgcgaaa ctccttctcg cttattgttt cctccggtcc cctgctcagg accataagac
     3721 ccccggtcct taaaaaaggc gctcctgctg caagcgccgc acccatggtc gcaaaggccc
     3781 tcgctgtaac cacatcgtac aacgaccatg atgaattctc gatctcctca atccttttat
     3841 cgatgacttc tgccccggcg agtcccaatg tcctgatgat atgcctgagg aaggaggcct
     3901 ttttttttta ccgactctac cagcgtgacc gatatgtcgg gtttgataat cttaatcgga
     3961 agtgctggaa atcccgcacc tga
//
LOCUS       DASSLD010000513         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_270835,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000513 DASSLD010000000
VERSION     DASSLD010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270835"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFK23_09290"
     CDS             <1..275
                     /locus_tag="VFK23_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009049752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7319316.1"
                     /translation="FIASRHGKARDSVELTVKDTGKGISPEFLPHIFDPFFSTKGEGG
                     TGLGLSVSYGIIKNHKGEIRVESKVGVGTTFTVELPCYHEEENKHG"
     gene            268..633
                     /locus_tag="VFK23_09295"
     CDS             268..633
                     /locus_tag="VFK23_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7319317.1"
                     /translation="MDKHSIMVIDDEKIVGDMAKLSLEQDGYEVETFLSAEPALARMK
                     EKKFDVVVTDYKMKGIDGMEVLRTVKKGYPGTTVIMITAFANLDAAIEALRGDVHDFF
                     PKPVKIRELKESIRRALGH"
     gene            824..>2012
                     /locus_tag="VFK23_09300"
     CDS             824..>2012
                     /locus_tag="VFK23_09300"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET7319318.1"
                     /translation="MKHPLDLKSIVSSLSRTFSRRKFPSDPAEFRAVFERFKSVLESN
                     NRALETITDMGEKLGGDYLFDIVYVKKAYASLKNDMETSLHAFDELTQGRHERLRESF
                     AHLDSLISSMVNETLPAAGAPVLFFEEIPWGVSQVVGGKNANLAEVKNQLHLPVPDAF
                     VITAAGFDAFVRQNRLDSHIEQLKQESSLSDSFLQELQELILHGEVPPEVSRRIQKAL
                     DKIRRRNNGAYLALRSSALDEDSGHSFAGQFKTVLNVPPELQEVERAYRKVLASLYSG
                     KAVAYQQQLGYDISALKMAVGCMVMVDAAASGVIYSITPEGDRDRLIISAAWGLGEAI
                     VEGKTETDLYLVRKGSEPELVRATYGNKQSMIVTGARGGTAEVQTPDELKARPCLTPE
                     QIMT"
BASE COUNT          478 a          576 c          595 g          363 t
ORIGIN      
        1 tctttatcgc atcccgccac ggcaaggccc gcgattccgt cgaactcacg gttaaggaca
       61 caggaaaggg catatcgccg gagttcctgc ctcacatctt cgatcctttc ttcagcacca
      121 agggcgaggg agggacgggc ctggggctgt ccgtcagcta tggtatcatt aagaatcata
      181 aaggagaaat cagggtcgag agcaaggtcg gggtcggcac gacctttacc gtcgaactgc
      241 cctgctatca cgaagaggag aacaagcatg gataaacaca gcatcatggt cattgacgac
      301 gagaagatcg tgggcgacat ggcaaagctg tcgcttgagc aggacggcta cgaggtcgag
      361 acgttcctga gcgccgagcc cgcgctggcg cggatgaagg agaagaagtt cgacgttgtc
      421 gtgaccgact ataagatgaa gggcattgac ggcatggagg tgctccggac cgtcaagaag
      481 gggtatccgg ggaccacggt catcatgatc accgccttcg ccaatctcga cgcagcaatc
      541 gaggcgctcc gcggggacgt gcacgacttc ttccccaagc cggtcaagat cagggagctg
      601 aaggagtcga tccgaagggc gctcggtcat tgatgacccg ggagcgacag agaacccttt
      661 tcgttattcc cggacgcttc catcgggaat ccagggttgc caatcgtctg agagaagcaa
      721 aggcgccgga tgccggcgcc ctgcactcgc gcagggcact cgtacccgct gggtgcaggc
      781 cagaaccagg acggaatgac gactgacgtg tgacaaggca tgtatgaagc atcccctaga
      841 ccttaaatcg atagtttcct ccctctcccg gactttctcg cgcaggaagt tcccctctga
      901 tcctgccgag ttcagggcag tattcgaacg cttcaagagc gtactcgaaa gcaacaaccg
      961 cgcactggaa accataacgg acatgggcga aaagctgggc ggcgactatc tcttcgatat
     1021 tgtttacgtc aaaaaggctt atgcctccct caaaaacgac atggaaactt cgctccatgc
     1081 tttcgatgag ctcacgcagg gcaggcatga gcggctgcgg gaatcttttg cacaccttga
     1141 ctcgctgatc agcagcatgg tgaacgagac gctgccggcg gcaggcgcac ccgttctttt
     1201 tttcgaagag atcccctggg gcgtgtcgca ggtcgtgggc ggcaagaacg ccaacctcgc
     1261 cgaggtaaaa aaccagctgc acctcccggt ccccgacgcc ttcgtcatca ccgccgcggg
     1321 ctttgacgcc ttcgtcagac agaatcgcct cgacagccat atcgaacaac tgaagcagga
     1381 gtcatcgttg tcggactcgt ttcttcagga actgcaggag ctcatcctgc acggtgaagt
     1441 tcctccggag gtcagccgca ggatccagaa ggcactggac aagatacgca ggcgcaacaa
     1501 cggcgcttac ctcgcgctcc ggagcagcgc cctggatgag gacagcggcc attcctttgc
     1561 cggccagttc aagaccgtcc tgaatgttcc tcccgagctc caggaagtgg aacgcgcata
     1621 ccgaaaagtg cttgccagcc tctattcagg caaggcggtc gcttaccagc aacagctggg
     1681 ctatgatatc agtgcgctca aaatggcggt cggctgcatg gtcatggtcg atgccgcggc
     1741 gagcggggtc atctactcga taacgccaga aggagaccgg gacaggctca tcatcagcgc
     1801 tgcctggggg ctgggagagg cgatcgttga gggcaaaacc gagacagacc tgtaccttgt
     1861 ccgaaaaggc agcgaacccg aactcgtgcg ggccacctac ggcaataaac aatcgatgat
     1921 cgtgacgggc gcaaggggag ggacggccga agtacaaacc ccggacgaac tgaaggcgcg
     1981 accctgtctc actcctgaac aaataatgac gc
//
LOCUS       DASSLD010000514         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_33746,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000514 DASSLD010000000
VERSION     DASSLD010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33746"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VFK23_09305"
     CDS             complement(<1..980)
                     /locus_tag="VFK23_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HET7319319.1"
                     /translation="MAEKQSPDKRPSRNAKKGLNLKTTMIAAKTGKSAGPAKSASQLT
                     TRRAIESQPAEQGRSNEKTFLIVGVGASAGGLEAFTKLLEHLPPDTGMAFVLVQHLAP
                     TKDSMLAELLSKATSMPVREVQDGMIAESDHVYVIPPNTALTIFHGKLRLLPRGETHT
                     QHMPVDSFFRSLAEDQGQNAVGVILSGTGSDGSLGIRAIKAEGGIVMAQDEQSAKYGG
                     MPKSAAQTGAVDFILPPEKIASELVRICRHPVMSLLTATKTGPLLQVREDELHKIFML
                     VRTATGVDFTYYKQATILRRITRRMVLHKIEALGQYVRYLQENPPEVGIL"
     gene            complement(1114..>2144)
                     /locus_tag="VFK23_09310"
     CDS             complement(1114..>2144)
                     /locus_tag="VFK23_09310"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HET7319320.1"
                     /translation="LTVNKISISEGRLVYADEKSGEKAKADNFDGTIRNLSYGGTDGA
                     GPFKDISFDGDLRCKTLTINNFTLTNLVMRAAGGNGILDIKLVSMHVFSGTGKGSIHM
                     DMTGSVPSYRVICTLSRCRVDELIQAFSSRKITEKSIEGLTDLSVDLTAQGKSADEAK
                     QSLSGDVSLKGENLMLYNMDIDALIPKYERSQNFNLVDVGAFFLAGPFGPVLTKSYNF
                     GSLYEELQGGKGNIRKLVSIWKVKNGVADAIDVALATKKYRIAMKGGLNFNNDRFVDV
                     TVAVLDKRGCAVYSQKVHGPFNKPKIDKVSIVKSITGPVSNALKDAWKFMGGECKTFY
                     SGSVAQPE"
BASE COUNT          433 a          584 c          559 g          568 t
ORIGIN      
        1 tacagaatgc cgacttccgg ggggttctcc tgcagataac ggacgtattg tcccaatgcc
       61 tctatcttat gaagtaccat gcgtcgggtg atcctgcgca gtatcgttgc ctgcttgtaa
      121 tacgtgaagt caacgcctgt ggccgtcctg accagcatga atattttgtg cagctcgtcc
      181 tctcttacct gaagcagcgg gccggtcttt gtggcggtga ggagggacat aacgggatgg
      241 cggcagattc ttacgagctc agaggctatt ttttcaggag gcaggatgaa atccacggcc
      301 cccgtctggg cggcgctttt gggcatgccg ccgtattttg ccgattgctc gtcctgggcc
      361 atgacgatcc cgccctcggc ctttatggcc cgtataccca gagaaccatc ggagccggtc
      421 cccgaaagga tgacaccgac tgcattctgt ccctgatcct cggcgagaga ccggaaaaaa
      481 gaatcaacag gcatatgctg ggtatgggtt tctccgcgcg gcagaagccg gagcttgccg
      541 tggaaaatcg tcagcgccgt attgggcgga atgacgtata catggtcgga ttccgcgatc
      601 atgccgtcct gtacttcgcg caccggcatg gaagtcgctt tagaaaggag ttcggccaac
      661 atgctgtctt tggttggggc aaggtgttga acgagcacga aagccatgcc cgtatcgggc
      721 ggaagatgtt ccaaaagctt cgtgaatgct tccaggccgc ctgcagaggc gccgactccg
      781 acaataagaa acgttttctc gttcgatcgg ccttgttcag caggctgact ctctatcgca
      841 cggcgggtcg tgagttgaga agccgatttt gccggccctg cgctcttacc ggttttggca
      901 gcaatcattg tcgtcttgag gtttaaccct ttcttggcgt ttcttgatgg acgtttatcc
      961 ggcgattgct tctcggccat gcgttctccc gatagtaggt attattcttt ggaaagttta
     1021 tgacacatta tattagcata gagagtgctc tctattatag aaaaaagcgc gtccccatcg
     1081 ccgcggaaac cgcattgcca tggatgatac ttatcactcc ggctgagcca ccgaacctga
     1141 gtaaaatgtc ttgcactctc cccccatgaa cttccatgca tctttcagcg cgttcgacac
     1201 gggtcccgta attgatttaa cgatacttac cttgtcgatc ttcggtttat tgaacggccc
     1261 gtgcaccttc tggctgtaaa cagcgcaccc tcgtttatcg aggaccgcta cggttacgtc
     1321 gacaaaccgg tcgttgttga aattcaatcc gcccttcatg gcgatccggt atttttttgt
     1381 ggcaagggcc acgtcaatcg cgtcggcaac gccgtttttt actttccaga tcgagacaag
     1441 ttttctgatg ttcccttttc cgccctgcaa ctcttcgtac aggcttccaa aattgtagct
     1501 cttggtgagc accgggccga aggggcccgc gaggaagaac gcccctacat ccacgaggtt
     1561 gaagttctgg cttcgttcgt acttcgggat aagggcatcg atatccatgt tgtagagcat
     1621 aaggttttcg cccttcaggg aaacgtcgcc actgagcgat tgcttcgcct cgtctgcgct
     1681 cttcccctgt gcggtcaggt ccacggaaag atctgtcagc ccttctatgc ttttttcggt
     1741 gattttcctg gacgagaatg cctgaataag ttcgtcgacc ctgcaccggc tgagagtaca
     1801 gattacccgg taagagggca ccgaccccgt catgtccata tgaatacttc ccttcccggt
     1861 cccgctgaaa acgtgcatac tgaccagctt tatatcgaga atcccatttc ctcctgctgc
     1921 tcgcattacg aggtttgtca gggtgaagtt gttgattgtg agtgtcttgc atctgagatc
     1981 cccgtcgaac gagatgtcct tgaatggtcc cgcgccatcc gtcccgccgt acgaaaggtt
     2041 ccgtatggtc ccgtcgaaat tgtccgcctt ggccttctcg ccggattttt catctgcgta
     2101 aacaagtctt ccctccgaga tggatatctt gttcaccgta agga
//
LOCUS       DASSLD010000515         3648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_434334,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000515 DASSLD010000000
VERSION     DASSLD010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434334"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..940)
                     /locus_tag="VFK23_09315"
     CDS             complement(107..940)
                     /locus_tag="VFK23_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7319321.1"
                     /translation="MILLGILSMMAALAWIWWWLVRHEAEVRHRWEGLAAHPRVTALR
                     TRFAPQIAFLQARLSPAEFIGLDLTIGAIILIGASWIFGGIAEDVVTGDPLTIIDRKI
                     AVWLHMHATPNLTEAMKFISLLASWPVVTGICLFMALYFAWKRSGYRLAAMTLTIPVG
                     MLMNGILKYAFHRSRPSWDDPILIIGSFGFPSSHAMAATLLYGFLAAFGVRKMRTWRW
                     RVLVVLSSGLLVVLIGFSRLYLGVHYLSDVLAGIAAGSAWLALCLSAVGTLRHHRSIL
                     R"
     gene            complement(1044..1817)
                     /locus_tag="VFK23_09320"
     CDS             complement(1044..1817)
                     /locus_tag="VFK23_09320"
                     /inference="COORDINATES: protein motif:HMM:NF042560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6675 family protein"
                     /protein_id="HET7319322.1"
                     /translation="MSGVVPLIVCAAILGGEVSSRADILPQPPCGGAPFPPYPDLENS
                     PAIRAWDRTESSRNWIPPACTGWMDSGFTTLVVVVGRFHHASGAEGLLRRIGAISEHT
                     VIRYWSTTHKRWQALILSAHALTDATGNRRREDFSPGEMAEGKSLYYQQEDNLVGKAI
                     YRMRIRSASPDRLVYDTINIGIMRYLLLTMFPPGGMQTITFLDRESQDVWRYYSIMRT
                     SGNASQLTVGYDASFINRAVAFYRYLAGVPTDKEPPAAP"
     gene            complement(1859..3274)
                     /locus_tag="VFK23_09325"
     CDS             complement(1859..3274)
                     /locus_tag="VFK23_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET7319323.1"
                     /translation="MNRTTIKPILFCFLSVAMLSLGGGCATLPKVSDAIDNASIQEPP
                     QILSSKGLLSPEKSKALMERLKKSVAPTDMVERYSAVIESVSGSPLTSGNKVTLLVDG
                     EATYAAMFKAMENAKDHINLETYIMEDDETGRRFSDLLLKKQAEGVQVNIIYDSRGSF
                     STPAPFFKRLRDAGIQVVAFNPINPLKAHKFKFWKLAPDHRKILIVDGKTAFTGGVNI
                     SAVYSNRPCGGSQSSGGKQDNVAAMSWRDTDVQVEGPAVAEFQKLFLDTWQRQKGPEL
                     SKGNYFPDLKAAGKVLVRVVGSSPGETNRLTYILYVSAIAFSENSLHLTNAYFVPDDE
                     TVNALIAAARRGVDVRIVLPGTTDSSMTLNAGRYFYSRLLKSGVKLYKRRGVMLHAKT
                     AVIDNVWSTVGSTNMDFWSFSTNDEVNAVILNKEFATEMEKMFARDIAESDEIRLEDW
                     KKRPVFNRFKEWFAHQFRRWL"
     gene            complement(3271..>3648)
                     /locus_tag="VFK23_09330"
     CDS             complement(3271..>3648)
                     /locus_tag="VFK23_09330"
                     /inference="COORDINATES: protein motif:HMM:NF024678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HET7319324.1"
                     /translation="DNTAALLNFIPKMDPETLLAGYRKVLASIYSPSEYFERAQAMIA
                     RLGARPKPRLAFSDYLALFRSFIRQGIFARYRGAYWRFLGKTFFRTPRHIGLAVTLAI
                     MGHHFFTLTRRLESGIPQGDATL"
BASE COUNT          804 a         1050 c         1032 g          762 t
ORIGIN      
        1 attgatccgg ccccttgctt ttgatcctgt tccgaaatct tccttacgaa atcgctgttg
       61 caagggtgta cgaagattac ggttggaatc gctcctgtct ttccccttac ctgagaatgg
      121 agcgatgatg ccggagggtt cccacggcgg acaaacaaag cgcaagccac gctgaccctg
      181 ccgcaatgcc cgcgagtaca tcgctcaagt aatgaacgcc gagataaagg cggctgaatc
      241 cgatcagcac gaccagcagg ccggaggaaa gaaccaccag tacccgccat cgccaggtcc
      301 gcatcttcct tacgccgaag gcggccagaa aaccatagag caaggtcgcc gccatcgcgt
      361 ggctgctggg gaaaccaaag cttccgatga taaggatggg atcgtcccag gatggccgtg
      421 aacgatgaaa cgcatatttc aaaatcccgt tcatcaacat gccgacgggg atggtcagcg
      481 tcatcgccgc gagacgatag ccggaacgct tccacgcaaa atataacgcc atgaacaagc
      541 agattcccgt cactacgggc catgaggcga ggagggagat gaacttcatt gcttcggtga
      601 ggttcggcgt ggcgtgcatg tgaagccaca ccgcgatttt cctgtcaatg atggtgagcg
      661 gatcgcccgt gaccacgtcc tcggcgatcc ctccgaaaat ccagctggcg ccgatgagga
      721 tgatggctcc aatcgtcaag tcgagcccta tgaattcggc cggtgacaga cgcgcctgga
      781 gaaacgcgat ctggggcgcg aatcgggtgc gaagggcggt aacccgtgga tgggcggcaa
      841 gtccctccca acgatgtcgt acctcagctt cgtggcgtac gagccaccac cagatccagg
      901 ccagcgccgc catcatcgac aggatgccga gcaagatcat gcttccgcct cccaaagcga
      961 tacgggacgg gcaaggattg atcttccgtg ccggtgcata cggctatgga tggcttgctt
     1021 gcggaggaga actcacgacc gaatcatggg gctgcaggag gctccttgtc cgtcggaacg
     1081 cccgcaagat aacggtaaaa tgccactgcc cggttgatga aggaagcatc gtatcccacg
     1141 gtcaactggc tcgcgttccc gctcgttcgc ataatgctgt aatagcgcca gacgtcttgc
     1201 gactcgcggt cgaggaaggt aatcgtctgc atcccgcctg gcggaaacat ggtcagcagc
     1261 aggtagcgca tgataccgat gtttatcgta tcgtacacga gccgatcggg agaggcgctt
     1321 cggatgcgca tccggtagat cgccttgccg accaggttgt cctcctgttg atagtatagg
     1381 ctctttcctt ccgccatctc gccaggggag aagtcttctc gacgtcgatt tccggtcgca
     1441 tccgtcaaag cgtgagcgct gaggatcagc gcctgccatc gcttgtgagt ggtcgaccaa
     1501 taccgtatga cggtatgctc ggagatcgcc ccgatacgcc ggagaagacc ctcggctccg
     1561 gaggcatggt ggaaacgccc taccaccacg accaatgttg tgaagcccga atccatccac
     1621 ccggtgcagg ccggcggtat ccagttgcga ctcgattcgg tgcggtccca ggccctgatg
     1681 gcaggtgaat tttccaggtc gggataggga ggaaagggtg cgcccccgca gggaggctgc
     1741 ggcaagatat cggcgcggct cgaaacttcg ccgccaagga tcgccgcgca tacgatcagg
     1801 gggacgaccc cggacaatct ccggaaaaga tgcttattca acattggtcg acggtcgatt
     1861 acaaccaacg ccgaaattga tgcgcaaacc actccttgaa cctgttgaac acaggtcttt
     1921 tcttccaatc ttccagccga atctcgtcgg attccgcaat atccctggca aacatcttct
     1981 ccatttcagt ggcgaattcc ttgttcaaaa tcaccgcgtt cacctcgtca ttggttgaaa
     2041 aactccagaa gtccatattg gtggaaccga cggtcgacca gacgttgtca atgaccgcgg
     2101 ttttcgcatg cagcatcaca ccgcggcgct tgtataactt cacacccgat ttcaaaagcc
     2161 tggaataaaa ataccgcccg gcattcaagg tcatggaaga atcggtggtt ccgggaagga
     2221 caatcctcac gtcaacccca cgcctggcgg ccgcgatgag agcgttcacc gtttcgtcgt
     2281 cggggacgaa atatgcattc gtcagatgga gcgaattttc cgaaaacgcg atggcggaca
     2341 catacagaat atacgtgagc ctgttggtct ctcccgggga actgccgacc actctcacca
     2401 agacttttcc ggctgctttc aggtccggga aatagttccc cttcgaaagt tccgggccct
     2461 tctgtcgctg ccacgtgtcg agaaacagtt tctggaattc ggccacggca gggccttcaa
     2521 cctgaacatc cgtgtcacgc caggacatcg ctgccacatt gtcctgtttc cccccggaac
     2581 tttggctccc cccgcaaggc cggttcgaat aaaccgcgct gatgttgacg cctcccgtga
     2641 aggcggtttt gccgtcgacg atcagaatct tgcggtgatc cggtgccagt ttccagaatt
     2701 tgaatttgtg ggccttcagt ggattgatcg ggttgaaggc tacgacctga atccccgcgt
     2761 cgcgcaggcg tttaaaaaac ggagcgggag tactgaagct gccccgactg tcataaatga
     2821 tgtttacctg gactccttcc gcctgctttt tcaacaacag atcggaaaat ctgcgacccg
     2881 tctcatcgtc ctccatgatg taggtttcaa ggttgatgtg gtccttggcg ttctccatcg
     2941 ctttgaacat ggcggcatag gtcgcctcac catcgacgag aagggtgact ttgtttccgc
     3001 tggtcagcgg gcttccgctc accgattcga tgacggcgct atagcgttcc accatgtccg
     3061 tcggggcgac ggatttcttc agccgttcca tcaaagcttt gcttttttca ggagatagca
     3121 gccctttcga cgacaggatt tgcggaggtt cctggatgct cgcgttatcg atcgcatcgg
     3181 agaccttcgg caaggtcgca catccaccac cgaggctcag catggcgacc gacagaaagc
     3241 agaaaaggat gggcttgatg gtcgtcctgt tcataatgtc gcatcccctt gaggaatacc
     3301 cgactccagc cggcgggtca gggtaaagaa atgatgcccc atgatggcca gcgtcaccgc
     3361 cagcccgatg tgtctgggtg tgcggaaaaa cgtcttcccg aggaagcgcc agtaggcccc
     3421 ccggtaccgg gcgaagatcc cctgccggat gaacgaccgg aacagcgcga ggtagtccga
     3481 gaaggcaagg cgcggttttg gcctggcccc cagacgggcg atcattgcct gggcccgctc
     3541 gaaatattcc gacgggctgt aaatggaagc gagtactttc cggtagcccg cgaggagcgt
     3601 ttccgggtcc atcttgggga tgaaattcag cagggccgcc gtattgtc
//
LOCUS       DASSLD010000516         3068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1084100, whole genome shotgun sequence.
ACCESSION   DASSLD010000516 DASSLD010000000
VERSION     DASSLD010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084100"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFK23_09335"
     CDS             <1..220
                     /locus_tag="VFK23_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319325.1"
                     /translation="TKRSVEIVRILKDLSREEGLTVIMVSHNPELARMADRQFEMRDG
                     EISERENLNWNMSERHGPERVPHYVAAE"
     gene            complement(273..2093)
                     /gene="typA"
                     /locus_tag="VFK23_09340"
     CDS             complement(273..2093)
                     /gene="typA"
                     /locus_tag="VFK23_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET7319326.1"
                     /translation="MIKRTDLRNIAIIAHVDHGKTTLVDTLLRQSGTFRDNERVQERV
                     MDNIDLERERGITIMAKNTSVHYNGVKINIVDTPGHADFGGEVERTLKMVDGVLLLVD
                     ASEGPLPQTRFVLKKALELGLPPILVINKIDRPDARISEVVNEVYDLFIDLDANDAQL
                     DFPIVFTNAREGVAKMSMEDESKDLKPLFELILKTIPAAEGDENAPLQLLVMNIDYND
                     YVGRLAIGKIFAGTAKVAEPIGVVNHTGAVVKTKITSLFEFQGLKRVDTASATVGDIV
                     ALAGIEGVNIGDTITDPENPKPMPRIAVDEPTISMLFSINTSPFAGKEGKYVTSRHLK
                     ERLEKELLYNVAIKVESGDATDSFKVLGRGELQLAILIEMMRREGFELSVSQPETITK
                     QRDGQTYEPMENLVIDCPEEFIGVVTQKLGTRKGRMTKMANNGHGRVRLEFRIPARGL
                     IGFRSEFLTDTRGTGLLNHLFDGYEPWQGPIAKRATGALVADRSGKTTTYALYHIQPR
                     GELFIKESTAVYEGMIVGENSRENDIDVNVIKEKKLTNMRASGADEALRLVPHRQLSL
                     EQALEFIKEDELVEVTPQTIRLRKKILESNKRPKNRDKMD"
     gene            2569..>3068
                     /locus_tag="VFK23_09345"
     CDS             2569..>3068
                     /locus_tag="VFK23_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7319327.1"
                     /translation="MDNTAPAISIVIPVLNEAAGINETIANLRRSAASGESEIIVVDG
                     DPSGSTVYAVEDDSVRRIISARGRATQMNRGAEAASGDILLFLHADTRLPGNALPLIR
                     ESMADERFVAGAFDLGFDTKRSIFRVTESYVFLRTRLTRVPFGDQAIFFRRDYFHDLG
                     GYREVP"
BASE COUNT          572 a          865 c          925 g          706 t
ORIGIN      
        1 cacgaagcgg tcggtggaga tcgtccgcat cttgaaagac ctgagcaggg aagagggttt
       61 gaccgtcatc atggtctcgc acaatccgga actggcgcgt atggcggatc ggcaattcga
      121 aatgcgggac ggagagatca gcgagagaga gaatttaaac tggaatatgt ctgagagaca
      181 cgggccggaa agagtcccgc attatgtcgc cgcagaataa actccatgat accgggcatg
      241 atgctttgaa gtcttagcgc gaacagcaag tctcagtcca tcttgtcccg gttcttcggc
      301 cgcttgttcg actcgaggat cttcttccgc agccggatgg tctgcggcgt tacttccacg
      361 agttcgtctt ccttgatgaa ctccagggcc tgctcgagcg ataactgccg gtggggcacg
      421 aggcggaggg cctcgtcggc gccggaggcc cgcatgttcg tgagtttctt ttcctttatc
      481 acgttcacgt cgatgtcgtt ttcccgggaa ttttcgccca cgatcatgcc ttcatacacc
      541 gcggtgctct ccttgatgaa cagctccccg cgcggctgga tatggtaaag ggcataggtc
      601 gtggtcttgc cggaccggtc cgccacgagg gcgccggtgg cgcgcttggc gatcgggccc
      661 tgccagggct cgtacccgtc aaagaggtgg ttcaggagtc cggtgccgcg cgtgtcggtc
      721 aggaactcgg aacggaaacc gatgaggccg cgcgcgggga tgcggaactc gagcctgacg
      781 cggccgtgtc cgttatttgc catcttcgtc atgcggcctt tgcgggtccc caacttctgg
      841 gtgaccacgc cgatgaattc ctcgggacag tcgatgacca ggttttccat gggttcatag
      901 gtctgcccgt cccgttgttt ggtgatggtc tcaggttgag acacggaaag ctcgaatccc
      961 tcgcgccgca tcatctctat cagaatggca agttgaagct cgccgcgtcc caggaccttg
     1021 aagctgtcgg tcgcatcgcc cgactcgacc ttgatcgcca cgttatagag gagttccttc
     1081 tcgagccgct ccttcaaatg gcgcgacgta acgtatttgc cctccttgcc cgcaaagggg
     1141 gaggtgttga tcgagaagag catggagatc gtcggctcat ccacggcgat gcgtggcatg
     1201 ggcttcgggt tctccgggtc ggttatggtg tcgccgatgt tcacgccttc aatgcccgcc
     1261 agggccacga tgtcgccgac tgtggcgctg gccgtgtcaa cgcgcttcag accctgaaac
     1321 tcgaagagcg acgtgatctt ggtcttgacc acggcgccgg tatggttgac gacgccgatg
     1381 ggctcggcga cctttgccgt gccggcgaag atctttccga ttgcaaggcg ccccacgtaa
     1441 tcattgtaat cgatgttcat gaccagaagc tggagcgggg cgttctcgtc gccttcggca
     1501 gccgggatcg ttttcaggat caattcaaag agaggtttca ggtctttgct ttcgtcttcc
     1561 atgctcattt tggcgacgcc ttcgcgggcg ttcgtaaaaa cgatgggaaa gtccagctgg
     1621 gcgtcatttg cgtcgaggtc gatgaacagg tcgtatactt cattgaccac ctcgctgatg
     1681 cgcgcgtcgg gacggtctat cttgttaatc acgaggatcg gcgggaggcc gagttcgagg
     1741 gccttcttga gcacgaagcg ggtctggggg agcggccctt ccgatgcgtc cacgaggagg
     1801 agcacgccgt ccaccatctt gagggtacgc tccacctcgc cgccgaaatc cgcgtgtccc
     1861 ggcgtgtcca cgatattgat tttcacgccg ttgtagtgta cggaggtgtt cttggccatg
     1921 atcgtgatgc cgcgctcgcg ttcgaggtcg atgttgtcca tgacgcgctc ctgaacgcgc
     1981 tcgttgtcgc gaaaggtgcc gctctgcctg aggagcgtgt ccacgagcgt ggtcttgccg
     2041 tggtccacat gggcgatgat ggcgatgttg cgcaggtcag ttctttttat cattcctttt
     2101 tccttatcct tttggctgaa aaaacggcag gtctgccgtg gtgcggcgca gatttgtaat
     2161 accgggtccc gatgcgtttc ccgaagctgg atttgatgct acgtttgagc gaagctgggt
     2221 tttataccac ttcttcgagc gctttgtcaa tgcaataatg gcgggcgggg cttgtctacg
     2281 ccgcttgctt gacaggcttc agaagcggtg ctaactttaa aaagggaata catcccggtc
     2341 tgtaggaccg agactgaagg agaaacagaa aaataccgcc gagaacgcgg aaagattccg
     2401 gagaaaagcg cggggcggag aagggatttg ctgtggtcct ccctaacgca gtaattcgcc
     2461 ttggtcctcc cccgtatgat ctcgaaaggc tttccacagc ttcctggcgg tatttttttg
     2521 cgaaatcgtc gccggcaaac ggacgttcgc cgctcccctg agaaaagctt ggacaacacc
     2581 gcacctgcaa tatccattgt cattcccgtc ctgaatgagg ccgccgggat caatgaaacc
     2641 atcgcaaacc tgcgccgttc agctgcgtca ggggaatcgg agatcatcgt ggtcgacggg
     2701 gacccctcgg gaagcacggt ctacgccgtc gaggacgact ccgtccggag aattatttcg
     2761 gcgcggggcc gggcaactca gatgaaccgc ggcgccgagg ccgcctcggg tgacatcctc
     2821 ctctttctcc atgcagacac ccgcctcccc gggaacgcgc ttccgctgat acgcgagtcc
     2881 atggccgacg agcgctttgt cgccggcgca ttcgatctcg ggttcgacac gaaacgaagc
     2941 atcttccgcg tcacagagag ctacgttttt ctccgcaccc gcctcaccag ggtccctttc
     3001 ggagaccaag ctattttttt ccgtcgtgat tatttccacg atctcggcgg gtaccgggag
     3061 gtccccat
//
LOCUS       DASSLD010000517         3730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1542704, whole genome shotgun sequence.
ACCESSION   DASSLD010000517 DASSLD010000000
VERSION     DASSLD010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542704"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            609..809
                     /locus_tag="VFK23_09350"
     CDS             609..809
                     /locus_tag="VFK23_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015846137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET7319328.1"
                     /translation="METGTVKWFNDAKGFGFITREKGGDVFVHFGDIQDSGFKSLAEG
                     QNVQFDVVDSPKGPKAANVVKL"
     gene            963..1343
                     /locus_tag="VFK23_09355"
     CDS             963..1343
                     /locus_tag="VFK23_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319329.1"
                     /translation="MRHKQNYREKQAQEKAALIALGLVSERHAGVSNIELRMTYYRGG
                     CGEVLMERTLCFRPADYAGFHMKCMEDGCADGGFDLAPVVACLAKARGKSVKGRLFCQ
                     GRNHASGRANIAYEVNIRYARQVK"
     gene            complement(1516..1686)
                     /locus_tag="VFK23_09360"
     CDS             complement(1516..1686)
                     /locus_tag="VFK23_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319330.1"
                     /translation="MGKPNFNFQKRQKELAKQKKNEEKRQRKLEKNTAKPREAAEGVP
                     AGAVDVNVETGG"
     gene            complement(1787..2026)
                     /gene="rpsU"
                     /locus_tag="VFK23_09365"
     CDS             complement(1787..2026)
                     /gene="rpsU"
                     /locus_tag="VFK23_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET7319331.1"
                     /translation="MEIRVQSRNIEKALRDLKKKLQLDGLFGEMKKRRHYEKPSVKKK
                     KKQLEAEKRRRKLLKRSRSSGSWTGPRPASLPASR"
     gene            complement(2017..2331)
                     /locus_tag="VFK23_09370"
     CDS             complement(2017..2331)
                     /locus_tag="VFK23_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET7319332.1"
                     /translation="MAKKLYVGNLSYGTHEEDIREAFAKVGEVLSATLIVDQANGMSK
                     GFGFVEMASDEDAAKAISTLNGTSLMDRTITVNEARPKTDRPRSGGRGGYGGNSGGRS
                     WR"
     regulatory      2874..2971
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      3057..3156
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3281..>3730
                     /locus_tag="VFK23_09375"
     CDS             3281..>3730
                     /locus_tag="VFK23_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7319333.1"
                     /translation="MNRSDRKYALYMMCLLLLGVFALWGCPKKSEVATAPETQREEAP
                     VAAQAPVEAPAPAPAENPAPRVQEVQEKAAAAAPSLRPVYFDFDQALIRPDAKEILKA
                     NAEWMKAHPQAKIKIEGNRDERGTREYNQALGQRRAANAKKFMADLGV"
BASE COUNT          826 a         1016 c         1018 g          870 t
ORIGIN      
        1 tcacggcgca tgcatacgca cggctgtcgc gaccgggatg ccccctcaac tgaaatgcac
       61 ctgttttagt cctttgattc ccctgtttcc ccatgcgaaa atttagtcgt gcgaccgagg
      121 acgagggtta aacatttcga actgtttgaa gccggctgtc ccgcttccgc agggagaggg
      181 acagatagag agagatccct ctcggcgagt tttcgaaatg taccgaggac aaccgagcgc
      241 gccgtgaaga attttcgccg ggcgcccttc tttgggtcac ctttcttgga gcgagcaaga
      301 aaggtgacga acatatgcag cggactagac aacaatgagg cgtgaacagg atgttgctcg
      361 aatgctattt atttccattc ggtagggaga agacccaaaa gacttttatc ggatgtatca
      421 caccttctcc ctgccgaaac tattttcact ttcgtaataa taactgttga cattgccggg
      481 cagaaggcgg ataatccttt gcagaagttc cggatgttct tgtagggaaa ccgcaaagac
      541 atataggctt tgcggtattt tttttgtcct ttccgtcgct tcaattcaca agaaggaggt
      601 agctcatcat ggagacaggt accgtaaagt ggttcaatga tgccaaaggc tttggcttca
      661 ttacccgtga aaagggcggc gatgtgttcg ttcatttcgg cgacattcag gacagcggct
      721 tcaagtcgtt ggccgaaggc cagaacgtgc agttcgacgt tgtcgactcg cccaaaggcc
      781 ccaaggcagc caatgtcgtg aagctctaag ctttcacgaa aaacattgaa gcaggccctg
      841 aatgatcagg gcctgctttt ttcatttcgt tccctctctt gcttccgtcg gatgcggcag
      901 gacgggtacc gtccgccttt cccggcgccg gattattcag acgacaaggc tgaaggagag
      961 acatgcgaca caaacagaat tatagggaga agcaggcaca ggagaaagcc gccctgatcg
     1021 ccctgggact ggtttccgag cgacacgccg gagtttcgaa catcgaactt cggatgacct
     1081 attaccgggg cggatgcggt gaggtcttga tggagcgcac gctctgcttc cggcccgccg
     1141 actacgccgg gtttcacatg aagtgcatgg aggatgggtg cgctgacggc ggctttgatc
     1201 tggcgccggt cgtcgcatgc ctggcgaaag cgcgcgggaa gtccgtcaag gggaggctct
     1261 tttgccaggg caggaaccat gcctcgggac gggccaacat cgcctatgaa gtcaatatcc
     1321 ggtacgcgag gcaggtgaaa taaaaacccc gcgtttgccc gggcagaaaa aaacccactc
     1381 gcgctttctg agtgggcctg gccgtcttca agattatttt ttcttgctct tcggtttctt
     1441 tacttccttt ttggcgcctt gccgtgatgc cgccatgcgt catcacctcc ctgttcccgc
     1501 aatcctgttc acccgtcagc cgccggtttc gacgttcacg tcgaccgctc cggcaggcac
     1561 gccttccgcg gcctcccggg gctttgccgt gttcttctcg agttttcgct gtcgtttttc
     1621 ctcgttcttc ttctgcttcg ccagttcctt ttgacgcttt tgaaaattga agttcggttt
     1681 gcccattgca ggactccttt aaataaacaa agggcgcgaa gaaatcacga atcccggcga
     1741 ggtcaccggc tttcttcgcg tccatcggct tcgcgcactg tgctctctaa cgggatgcgg
     1801 ggagagacgc cggacgcggc cccgtccacg aaccggatga ccgcgaacgc ttgaggagct
     1861 tccgccttcg tttctcggcc tccagctgct tcttcttttt cttgaccgag ggcttttcgt
     1921 aatggcgccg cttcttcatt tccccgaaga ggccgtccag ctggagcttc ttcttcaggt
     1981 ccctcagagc cttctcgata ttccgggact ggacccttat ctccaagacc tgcctccgct
     2041 gttaccgccg tagccgcccc tgccgccgga cctcgggcgg tcggtcttcg gacgggcctc
     2101 gttcacggtg atcgtccggt ccatgaggga cgtgccgttc aaagtggaga tcgccttagc
     2161 cgcgtcctcg tcagacgcca tctccacgaa accgaaccct ttggacatgc cgttcgcctg
     2221 atcgacaatc agcgtggccg acaggacctc gccgaccttc gcgaaggcct cccggatatc
     2281 ctcttcgtgg gtaccgtacg acagattacc gacatacaat tttttcgcca tgatttcctc
     2341 ctggtgtttc ctgatttacg ccggcggtcg atgccgccgg gcgctttttc tttttctccg
     2401 cattcgcgcg tatccccgaa ggggcgcgga caggcggtct tttgcaaaca gcatcgttcg
     2461 ccaacctggg tatgcagtcg cttttttcgt gagaaaaggt gagagcagag agagtcagtg
     2521 tacgatgagg gtgcagagcc ttcatcccgt tttggtgaac gggaggggct tgctccgttg
     2581 acgatctgta tgttcattat acagcattcc ccgtccgatg caaggattaa agtgaaggaa
     2641 gaagtaaaaa attagaacac catgcggaat gcggaattcg gaattcgggc gaacctctca
     2701 tgggcaagta gcacctccgc gccggtccct tcgttcgtac ataatttctt cccagtggcg
     2761 aaaaatttca ggcaaagaag ctggacagaa tattgccgaa atctaacact tgacaacata
     2821 tttccctaat gctaaagttc tcatgctctc aaactaaata gtacaaatca ttactttgct
     2881 cagagcaaac cctgggaaac cgggggacgc agagcttcga ggctaaagaa accggcatcc
     2941 cgccgtcttc catgccagtc gagccgccaa aggacacaat tctttggcgg ctttttattt
     3001 atgcggaagg aagaggaaca ctaaatggct taaggcgcac gaataaatta ttattacttt
     3061 gctcagagca aaccccggga aaccggggga cgcagagcta cgaggctaag gaaaccggca
     3121 gatcctgcgg tctttcatgc cagtcgagcc gccaaaggat gaggcatctc tcttttggcg
     3181 gctttttatt tttaacacga aggaggtgag gctggaaaag cagagatttc aaaaaacgat
     3241 gcagtatatg catcgtaatc ttcgataaca ggaggttcgt atgaacagat cggacaggaa
     3301 gtatgcattg tacatgatgt gtctgttgtt gcttggcgtc tttgctctct ggggatgtcc
     3361 aaaaaagagc gaggtcgcca cggctcccga aacgcagcgg gaagaggccc cggtcgcggc
     3421 ccaggccccg gttgaagccc cggcacctgc cccggctgag aatccagcgc ccagagtcca
     3481 ggaagtccag gaaaaggccg ctgccgcagc tccgtcactg cggcccgtgt atttcgattt
     3541 cgaccaggcc ctgatccgtc ctgacgccaa ggagatcctg aaggcgaatg cagaatggat
     3601 gaaagcgcac ccgcaggcca agatcaagat tgagggcaat cgcgacgaga gagggaccag
     3661 ggaatacaat caggcgctcg gccagcggcg tgcggcaaat gcaaaaaaat tcatggccga
     3721 cctgggagtt
//
LOCUS       DASSLD010000518         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1448779, whole genome shotgun sequence.
ACCESSION   DASSLD010000518 DASSLD010000000
VERSION     DASSLD010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448779"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VFK23_09380"
     CDS             <1..828
                     /locus_tag="VFK23_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET7319334.1"
                     /translation="ALLPLDQIGFTGDTLTRFNAMIERPHGIILVTGPTGSGKTTTLY
                     AALSKINSPDKNIITIEDPIEYQLKGIGQIQVNAKINLTFAAGLRSVLRQDPDVILVG
                     EIRDSETAEIAIQAALTGHLVFSTLHTNDAAGAVTRLLDMKVEPFLISSSVIAILAQR
                     LVRVLCKECREAYPITAGEIAELEIKTVAKGATVYRAKGCETCFHTGYLGRSAIYELL
                     TVDDEMRQLIMKNTDAATIKALAMNRGMRTLRQDGAARVLAGITSVDEVVRVTQREA"
     gene            949..>2131
                     /gene="gspF"
                     /locus_tag="VFK23_09385"
     CDS             949..>2131
                     /gene="gspF"
                     /locus_tag="VFK23_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HET7319335.1"
                     /translation="MPVYEYKGVSAAGAKVSGVQDAESLKAVKSKLRKDGIIVLEIHE
                     GASLRSVRRGSSAIRFVARVKLGDLANATRQLATLLSSGLPLMEALSVLVEQEENEGL
                     KAAMSSVRDSVREGAALADALKENPKAFSQLYVNMVSAGETSGTLEITLDRLADFLDE
                     QVLFRGRISAALAYPAFMTVIGAAMLFFIFSFVMPRVVGMFTDMKQQLPMITLILLAT
                     VRFLSNFWWAILIVIAGGVYYLRRYLRTQAGREAFDARILRVPVFGNLIRMIAVSRFT
                     RTLGTLLEGGVPALVALDIVKSVVGNTVLANAIQEARENVREGEPIADPLKRSGLFPP
                     VVVQMVAVGEKSGELEKMLLKISDSFDRTVETRITGLMALMEPVIILTMGLIIGFIVI
                     AI"
BASE COUNT          438 a          643 c          648 g          402 t
ORIGIN      
        1 gccctcctgc cgctcgacca gatcggcttt acgggagaca ccctgaccag gttcaacgcc
       61 atgatcgagc gcccccacgg gatcatcctc gtgacgggcc ccacgggaag cggtaagacc
      121 acgacgctct acgcggcgct ctcaaagatc aactcgcccg ataagaacat catcacgatc
      181 gaggacccca tcgagtacca gctgaagggc atcggccaga tccaggtcaa cgccaagatc
      241 aacctgacct tcgccgcggg cctgcggtcc gtgcttcgcc aggacccgga cgtgatcctc
      301 gtgggcgaga tccgcgactc ggagaccgcc gagatcgcca tccaggcggc cctgacggga
      361 cacctcgtgt tctccaccct ccatacgaac gacgctgccg gagccgtgac gaggcttttg
      421 gacatgaagg tcgagccgtt cctcatctcc tcctcggtca tcgcgatcct cgcccagcgg
      481 cttgtgcgcg tgctctgcaa agaatgcagg gaggcctacc cgattacggc cggcgagatt
      541 gcggagctcg aaataaagac cgttgcgaag ggggcgacgg tgtaccgtgc aaaggggtgt
      601 gaaacctgtt ttcataccgg ctacctcggc aggagcgcga tctacgaact cttgaccgtg
      661 gacgacgaaa tgcgccagct catcatgaaa aacaccgacg ccgccacgat caaggcgctg
      721 gccatgaacc ggggcatgcg cacgctcagg caggacgggg cggcaagggt cctcgccgga
      781 atcacgtccg tcgatgaagt ggtgagggtg acgcagaggg aagcgtaata aaaagcacga
      841 aattcgaagt actaaattct aaacaatatc gaaattcaaa tttcccacat cggatgcaat
      901 gagtctattt aggatttaga acttggaatt tagaatttgc attaaactat gcccgtttac
      961 gaatataaag gcgtttccgc cgctggagcg aaggtctccg gcgttcagga tgccgaaagc
     1021 ctgaaggcgg tcaaatcgaa gctgcgaaaa gacggaatca tcgtgctgga gattcacgag
     1081 ggcgcgtctt tgcgttccgt ccggcgcggt tcttccgcca tacgattcgt cgcccgggtg
     1141 aagctcggcg atcttgccaa cgcgaccagg cagcttgcca cgctcctgtc ctcgggtctt
     1201 ccgctgatgg aggccctgtc ggtgctcgtg gagcaggagg agaacgaagg cctgaaggcg
     1261 gccatgtcgt cggtgcggga ttcggtacgc gagggcgccg cccttgccga cgccctcaag
     1321 gagaacccca aggccttctc gcagctctac gtcaacatgg tctcagcagg agagacgagc
     1381 gggaccctcg agatcacact ggaccggctt gccgatttcc tggacgagca ggtcctgttc
     1441 cgcggcagga tttccgcggc actggcctat ccggccttca tgacggtcat cggcgcggcc
     1501 atgctcttct tcatcttcag cttcgtcatg ccgcgggtgg tgggcatgtt cacagacatg
     1561 aagcagcagc tcccgatgat cacattgata cttctcgcca cggtgcggtt cctttcgaac
     1621 ttctggtggg cgatcctcat cgtcatcgcc gggggcgtct attatctccg gcggtatctg
     1681 cggacgcagg cgggaagaga ggccttcgac gcgcgcatcc tgagagtgcc ggtcttcggg
     1741 aacctcatcc gcatgatcgc cgtttcgcgg ttcacgcgca ccctgggaac cctgctcgag
     1801 ggcggcgtgc cggcgctcgt cgccctcgat atcgtcaaga gcgtcgtggg caacacggtc
     1861 ctcgcgaacg cgatccagga ggcgcgggag aacgtgcggg aaggcgagcc tatcgccgat
     1921 cccttgaagc ggagcggcct gttcccgccg gtggtcgtgc agatggtcgc cgtcggcgag
     1981 aagagcgggg aactcgagaa gatgctgctg aagatatcgg actccttcga ccgcaccgtc
     2041 gaaacacgca tcacgggcct catggcgctc atggagccgg tgattatcct gaccatgggg
     2101 ctcatcatcg ggttcatcgt aatagccatc a
//
LOCUS       DASSLD010000519         2518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1575392, whole genome shotgun sequence.
ACCESSION   DASSLD010000519 DASSLD010000000
VERSION     DASSLD010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575392"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFK23_09390"
     CDS             <1..244
                     /locus_tag="VFK23_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319336.1"
                     /translation="CTQPHLVTPDYIFDEGYCTWRGVTPSDHIDSVREREQLAQLAKT
                     DPKQYFKEIISWGNSRMARLAQEGWRKAHEREPVKT"
     gene            219..404
                     /locus_tag="VFK23_09395"
     CDS             219..404
                     /locus_tag="VFK23_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319337.1"
                     /translation="MRESLSKHKPRKSSGMRLASVLDLITICPKCGGEVGLWSEEAET
                     LCIFCQYRVFEKERTVH"
     gene            566..1144
                     /locus_tag="VFK23_09400"
     CDS             566..1144
                     /locus_tag="VFK23_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HET7319338.1"
                     /translation="MPYAAKDYSKLVGMEGFSETLLKNHFTLYQGYVTNTNKVLDTLD
                     QMLKDGKTATPEFAELKRRLGWEWNGMRLHEYYFENLGGKGGMDKGGALAKGLAASFG
                     SVEAWEKDFRATGAMRGIGWTVLYRDAASGRLINFWVNEHDVSHPAGCTPLLIMDVFE
                     HAFMLDYGLKRADYIEAFFKNINWQAVESRMK"
     gene            1161..1655
                     /locus_tag="VFK23_09405"
     CDS             1161..1655
                     /locus_tag="VFK23_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HET7319339.1"
                     /translation="MPNTEQNLQDAFAGESQANRKYLAFAKKAEEEGFEQVARLFRAA
                     AAAETIHAHNHLKALAGVKSTRENLMAAISGESYEFQQMYPQMIDDAKTEGQDYALRS
                     FNLANEVEKVHAALYKKALEDLGKNASADYYVCSVCGYTAEGEAPDDCPVCRAKKQAF
                     RKAE"
     gene            1659..2228
                     /locus_tag="VFK23_09410"
     CDS             1659..2228
                     /locus_tag="VFK23_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HET7319340.1"
                     /translation="MKVVAFNGSPCPGGNTEVLLEGALRPLRDAGHSIRVFNLYDLNI
                     KPCQDCGGCAKTGVCVHEDAMVDISVKIREAERIILASPIFFHNLSTQAKIMIDRCQA
                     FWCEKYVLKKSIPAGPRGRKGLLLLVGGRTEEASAKCAAETGEAFFRTVSVPEHETLS
                     FGGIDAKGDILKHPTALKLAAEAGKRLGL"
BASE COUNT          606 a          691 c          716 g          505 t
ORIGIN      
        1 gtgcactcag ccccacctgg ttacacccga ctatatcttc gacgagggct actgcacgtg
       61 gcggggagtc acgccgtcgg accatattga ctccgtgcgg gagcgggaac aactggccca
      121 gctggccaag acggacccga agcagtactt caaggaaatc atctcctggg ggaacagccg
      181 gatggcgcgg ctcgcgcagg aaggatggag gaaagctcat gagagagagc ctgtcaaaac
      241 ataaaccgag gaaatcgtcc ggcatgcggc tcgccagcgt tctcgatctc atcaccattt
      301 gccccaaatg cggcggtgag gtgggactct ggtcggaaga agcggagacg ctctgcatct
      361 tctgtcaata ccgggtcttt gaaaaagaac ggacggtcca ttgataacga aatacgatac
      421 agcattctag gtcaaaaccg gcagccgaac ggcgaaaggt cgtctcggct gcttttttgt
      481 acaaccgcct ttcctttagt caaccccttt tttgatatac ttagagcatt gtttaaaaac
      541 ttcatgcaac cgggaggaaa ccatcatgcc atatgcggcc aaggactatt cgaagctcgt
      601 cggaatggaa ggattcagcg aaacactctt gaagaaccat ttcacactgt atcaggggta
      661 tgtcacgaac acgaacaaag tgctcgacac gctcgaccag atgttaaagg acggcaagac
      721 ggcaacgccg gaatttgccg agctcaaacg cagactcgga tgggaatgga acggcatgcg
      781 tctccatgaa tactatttcg aaaaccttgg cggcaagggc ggcatggaca agggtggggc
      841 gctcgcaaaa ggcctggctg cgagctttgg gagcgtcgag gcctgggaaa aggatttccg
      901 ggcgaccggc gccatgcgcg gcatcggttg gaccgtcctc taccgggacg ctgcttcggg
      961 acggctcatc aatttctggg tgaacgagca cgacgtttcc catccagcgg gatgcacgcc
     1021 ccttctgatc atggacgtct tcgagcatgc cttcatgctc gactacgggc tcaagcgggc
     1081 cgactatatc gaggcgtttt tcaaaaacat caactggcag gcagttgaat cacggatgaa
     1141 atagaaagga gagcatcact atgccgaaca cggaacagaa cctgcaggac gcgttcgccg
     1201 gcgagtccca ggcgaacagg aaatacctgg cctttgcgaa aaaggccgag gaggagggct
     1261 tcgagcaggt ggcgcggctt ttccgggcag cagccgcggc cgagaccatc catgcccaca
     1321 accacctcaa ggccctcgcc ggcgtcaagt cgaccaggga gaacctgatg gctgccatca
     1381 gcggggaatc ctacgagttc cagcaaatgt atccacagat gatcgacgac gcgaagacgg
     1441 agggccagga ctacgcgctt cggagcttca atctggcgaa cgaagttgaa aaagttcacg
     1501 ccgccctgta caaaaaggcg ctggaggacc tcggcaaaaa cgcgtcagcc gattattacg
     1561 tgtgctcggt ctgcggctat accgccgagg gagaagcgcc ggatgattgc ccggtttgca
     1621 gggcaaagaa gcaggcgttc cgcaaggcag agtgagattt gaaggttgtc gcgttcaacg
     1681 gcagcccctg tccgggcggc aataccgaag tcctgctgga gggagcgctc aggccgctcc
     1741 gtgatgccgg acacagcatc agggtcttca acctctacga cttgaatatc aaaccctgcc
     1801 aggactgcgg cggctgcgca aaaaccggcg tctgcgttca cgaagacgcc atggtggata
     1861 tttccgtaaa aatcagggag gccgagagaa tcattctcgc ctccccgatt tttttccaca
     1921 acctgagcac ccaggcaaaa atcatgatcg accggtgtca ggcattctgg tgtgagaaat
     1981 atgtgttgaa gaagtccata cccgcggggc cccgcgggag aaaaggcctt ctgctgctcg
     2041 tcggaggaag gacggaagaa gcaagcgcga agtgcgccgc agagaccggc gaggcctttt
     2101 tcaggaccgt gagcgtgccc gaacacgaga ccctctcctt cggcggcatc gacgcgaagg
     2161 gagacattct gaagcatccg acggcgctca agctggctgc cgaggcgggg aagcgactgg
     2221 gcctctgaaa cgccgctttt tttagctgtc gcgtctttct tccggagctt ggacctcttt
     2281 tcatcccctt cacgccgcag tctgttgtcc tcaccataat ggtttataat acgaaacagg
     2341 tttgtcccac cggaatctga tcttttttat tgcagtcaca gcttttcttt gttacaatct
     2401 gctgcaacaa gctctcatgc aatagtctta aagcgtttgt aaccctttgt aactgttctc
     2461 gtccttattc acctacgaat cacttccctc gtgctcttgg atctcaggac gccgcact
//
LOCUS       DASSLD010000520         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_281015,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000520 DASSLD010000000
VERSION     DASSLD010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281015"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1990
                     /gene="uvrB"
                     /locus_tag="VFK23_09415"
     CDS             2..1990
                     /gene="uvrB"
                     /locus_tag="VFK23_09415"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET7319341.1"
                     /translation="MSEFKPTGDQPRAIDMLSEGIVKGIRDQVLLGVTGSGKTFTMAN
                     VVERIQKPTLVIAHNKTLAAQLYNEFKELFPENAVEYFVSYYDYYQPEAYLPTTDTYI
                     EKDSSINEQIDRMRHSATNSLFERNDVIVVASVSCIYGLGSPEAYHGMLLFLEKGQHI
                     ERNDILRKLVDIQYTRNDFDFQRGTFRVRGDVVEIFPASHESTAVRIELFGDEIDTIS
                     EFDPLLGTPLRRLEKIAVYPNSHYVIPEARMKTAIDGIHEELRERIQHFRRMNKLLEA
                     QRIEQRTMFDLEMIQEMGYCHGIENYSRHLSGRAPGEPPPTLLDYFPKDYLLFVDESH
                     ATIPQIGGMYNGDRARKTTLVEYGFRLPSALDNRPLNFQEFEERVSQAVYVSATPAEF
                     EIEKSGGRIIEQVIRPTGLTDPRIVVRPVAGQVDDLLNEIRKRAEARERVLVTTLTKR
                     MAEDLTEHYKELNVKVAYLHSDVDTLERVDIIRDLRMGKYDVLIGINLLREGLDIPEV
                     SLVAILDADKEGFLRSARSLIQTMGRAARDVNGEVILYAENMTDSMQNAIAETNRRRT
                     VQEEYNKEHGITPVSIKKSIQATLRTVYEQDYFTVPMAAEEAGEYVPTVNIPQLIASL
                     EKQMRAAAKELDFETAAELRDKIRSLRQQEMAVGVKVE"
     gene            2214..>2602
                     /gene="coaD"
                     /locus_tag="VFK23_09420"
     CDS             2214..>2602
                     /gene="coaD"
                     /locus_tag="VFK23_09420"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018691544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET7319342.1"
                     /translation="MKHIAVYPGTFDPVTKGHIDLVERSLTIFDELIVAVAANPKKKP
                     LFSLKERIDMFRKVVARYRRVRIEGFDGLLVDYVREKKASGIIRGLRAVSDFEYEMQM
                     ALMNRRLDNTIETVFLMPSEEYSFITST"
BASE COUNT          580 a          776 c          784 g          462 t
ORIGIN      
        1 catttcggaa ttcaaaccga ccggcgacca gccccgggcc atcgacatgc tttccgaggg
       61 catcgtgaag ggcattcgcg accaggtcct tctgggcgtc accggctcgg gtaagacctt
      121 cactatggcg aacgtcgtgg agcggatcca gaagcccacg ctcgtgatcg cgcacaacaa
      181 gactctcgca gcccagctct acaacgagtt caaggagctc ttccccgaga acgcggtcga
      241 gtacttcgtc tcgtactacg actattatca gcccgaggcg tacctgccga ccacggacac
      301 ctacatcgag aaggactcct ccatcaacga gcagatagac cgtatgcggc actcggcgac
      361 gaattcactc ttcgagcgga acgacgtgat tgtcgtggcc tcggtctcgt gcatctacgg
      421 cctgggctcg cccgaggcct atcacggcat gctgctgttt ctggaaaagg ggcagcacat
      481 cgagcggaac gacatcctgc ggaagctcgt ggacatccag tatacgcgga acgatttcga
      541 ttttcagcgc ggcacctttc gggtcagggg cgacgtggtc gaaatctttc ccgcctccca
      601 cgagagcacg gcagtccgca tcgagctgtt cggcgacgag atcgatacga tctcggagtt
      661 cgacccgctc ctggggacgc cgctccgcag gctcgaaaag atcgccgtct acccgaacag
      721 ccattacgtc atccccgagg cgcgcatgaa gacggccatc gacggcatcc acgaggagct
      781 ccgggaacgg atccagcact tccggaggat gaacaagctc ctcgaggccc agcgcatcga
      841 gcagcggacc atgttcgacc tcgagatgat ccaggagatg ggctactgcc acggcatcga
      901 gaactactca cgccatcttt ccggacgcgc gccgggcgaa ccgccgccga cgctgctcga
      961 ttatttcccc aaagactacc tcctcttcgt cgacgagtcg cacgccacga tccctcagat
     1021 cggaggcatg tacaacgggg accgggccag gaagacgacg ctcgtggagt acggcttccg
     1081 gcttccctcg gcgctggaca accgtccgct caattttcag gagttcgaag agcgggtgag
     1141 tcaggccgtc tacgtttcgg cgacccccgc cgagttcgag atcgaaaaat ccggaggcag
     1201 gatcatcgaa caggtcatca ggcccacggg actcaccgat cccaggatcg ttgtcaggcc
     1261 ggtcgcggga caggtggacg acttgttgaa cgagatccgg aagcgcgccg aggcgcgcga
     1321 gcgcgtgctc gtgaccacgc tcacgaagcg catggcggag gacctgaccg aacattataa
     1381 ggaactgaac gtgaaggtgg cgtacctgca ctccgatgtc gacaccctcg aacgggtgga
     1441 catcatccgg gaccttcgca tggggaaata tgacgttttg atcggcatca acctcctgcg
     1501 cgaaggcctg gacatccccg aggtttccct cgtggcgatc ctcgatgcgg acaaggaagg
     1561 gtttctgcgc tcggcgcggt cgctcatcca gaccatgggc cgcgcggccc gagacgtgaa
     1621 cggagaggtc attctctacg ccgagaacat gaccgattcc atgcagaacg ccattgccga
     1681 gacgaaccgg cgacgcaccg ttcaggaaga atacaacaag gaacatggca tcacgccggt
     1741 gtcgatcaag aagagcatcc aggccaccct ccgcaccgtg tacgagcagg attacttcac
     1801 cgttccgatg gcagcggaag aggcgggcga gtatgtgccg acggtgaaca tcccgcaact
     1861 catcgcatcg ctcgagaaac agatgcgcgc tgccgcgaag gagcttgatt tcgagaccgc
     1921 ggcggagctg cgcgacaaga tccggtcgct ccggcagcag gagatggccg tgggggtgaa
     1981 ggtggaatga ggtgattgca caatgtggaa ttcaccctgc actcgcgcgc aaggcactcg
     2041 taagcgctgg gcgcaggcgg aatgcggaat gcggaatgcg tgtagtaagg agaataggat
     2101 ctgccggtgg taaagaatgc gagttcttcg ataacagtct tgagcgcagg agcgtcttgt
     2161 gctagactgg gccggatagt cccagagtat tgcatcgaga ggagaccgac atcatgaaac
     2221 acatcgccgt ctatcccgga accttcgacc ccgtcacgaa ggggcacatc gacctcgtgg
     2281 agaggagcct tacgatattc gacgagctga tcgtcgccgt cgctgcaaac ccgaaaaaga
     2341 aaccgctctt ttccctcaaa gaacgaatcg acatgttcag gaaggtcgtg gcccgctacc
     2401 gtcgggtgcg gatcgagggg ttcgacgggc tcctggtcga ttacgtccgg gagaaaaagg
     2461 catcgggcat catccgcggc ctgcgcgcgg tctccgactt cgagtatgag atgcagatgg
     2521 ccctcatgaa tcggcgtctg gataatacga ttgagaccgt cttcctgatg ccgagcgagg
     2581 agtattcctt cattacctcg ac
//
LOCUS       DASSLD010000521         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1318728, whole genome shotgun sequence.
ACCESSION   DASSLD010000521 DASSLD010000000
VERSION     DASSLD010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318728"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VFK23_09425"
     CDS             <1..329
                     /locus_tag="VFK23_09425"
                     /inference="COORDINATES: protein motif:HMM:NF016083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HET7319343.1"
                     /translation="PVFFRESGLPTGAILFALPFIVGLLTGLTVGFVGATFPIITAML
                     GGHPDPGAVTFAFASGFAGVMLSPSHLCLLLTIRYFKADTAGTYRLMYVPVLLVFAVG
                     LVRLWW"
     gene            540..1733
                     /locus_tag="VFK23_09430"
     CDS             540..1733
                     /locus_tag="VFK23_09430"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HET7319344.1"
                     /translation="MITGDILADSQKYIMNTYGRQPLVLVKGRGTKVYDSDGKEYIDF
                     VSGVAVCNLGHCHPRVVVALQKQAQRLMHVSNHYHNEPQINLAKALVQHSFADKVFFC
                     NSGTEAIEAAIKLARRYAREILKEDRYEIITMRGSFHGRTLGALAATAQDKFHKGFEP
                     VAPGFRYVPFNDVPSVEAAINEKTCAVLVEPVQGEGGVNVPSDDYLPALRYLCERSNI
                     LFILDEIQTGMGRTGRLFAYEHAGITPDVVALAKGLGAGMPIGALLATEKAAVALAPG
                     SHGSTFGGNPLACATALASLEALIEDNIIIPAVEYLGGYLLDGLRGIQRKYSFVKDVR
                     GKGLLVGMELDFPGKDIVTACIKEGLLINCTMDKVLRFMPPLVITEAEIDRLIGVLDG
                     IFAKR"
     gene            1833..>2082
                     /locus_tag="VFK23_09435"
     CDS             1833..>2082
                     /locus_tag="VFK23_09435"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425292.1"
                     /note="catalyzes the formation of L-citrulline from
                     carbamoyl phosphate and L-ornithine in arginine
                     biosynthesis and degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET7319345.1"
                     /translation="MKKDLLAVSDLAPKDIDNILKRAAVLKKMLRRGKVRPTLKGKTL
                     GMIFEKSSTRTRVSFEVGMYQLGGLALFLSSADLQIGRG"
BASE COUNT          420 a          632 c          599 g          431 t
ORIGIN      
        1 tacccgtttt cttcagagaa agcggcctgc ccacgggagc catcctgttc gcgctcccct
       61 ttatcgtcgg gcttttgacc ggactcaccg tcggctttgt cggcgccacc ttcccgatca
      121 tcacggccat gctggggggg cacccggacc ccggagcagt caccttcgcc tttgcctccg
      181 gctttgccgg cgtcatgctc tccccctccc acttgtgtct gcttttgacg atcagatatt
      241 tcaaggcgga cacagccgga acctaccggc tcatgtatgt gccggtgctt ctcgtctttg
      301 ccgtggggct cgtcaggttg tggtggtaag gattcacagc agctgtcgtt cccgaatgat
      361 cctatcggga atccatcccc gattgaaccg ttcgaggata tgttttgaat tagccttgaa
      421 aaaagccgct ggattccctc ttcagcgtgg cccgtttcat cggccatcgc gggaatgaca
      481 acccgagtag atacaatttc cgtttgattt ttcaggcagg ttatgctact ttcccttcca
      541 tgatcaccgg cgatatcctt gcggattcac agaaatatat catgaacact tacggccgtc
      601 agccgctcgt gctcgtcaaa ggcagaggga cgaaggtgta cgactccgac ggcaaggaat
      661 acatcgactt cgtctccggc gttgccgtgt gcaacctggg ccattgccat ccgcgggtag
      721 tcgtggcgct ccagaagcag gcgcaacggc tgatgcacgt atcgaaccat taccacaacg
      781 agccgcagat caacctggcg aaggcgctgg tgcagcactc gttcgcggac aaggtgttct
      841 tctgcaattc gggcaccgag gcaatcgagg ccgcgatcaa gctcgcgcgg agatacgccc
      901 gggagatcct gaaagaggac cggtatgaga tcatcaccat gcggggttcg tttcacggac
      961 ggacgctggg cgccctcgcg gcaacggcgc aggacaagtt ccacaagggc ttcgagcccg
     1021 tggcccccgg gttccgttac gtcccgttca acgacgtccc gtcggtcgaa gcggctatca
     1081 acgaaaagac ctgcgcggtg ctcgtcgagc ccgtccaggg cgagggtggc gtcaacgtcc
     1141 cctccgacga ttacctgccg gcactgcggt atctttgcga acgcagcaac atccttttta
     1201 tattagacga gatccaaacc ggaatgggcc ggacgggcag gctcttcgcc tacgaacacg
     1261 ccggcatcac tcccgacgtc gtggcgctcg cgaagggcct cggcgccggg atgccgatcg
     1321 gcgccctgct cgcaacggaa aaagcggctg tcgccctcgc acccggctca cacggctcga
     1381 ccttcggcgg gaatccgctt gcctgcgcga cggcgctggc ttcgctcgaa gcgctgatcg
     1441 aggacaacat aatcatcccc gccgtggagt atctcggagg atatcttctg gacgggctcc
     1501 gtggcatcca gagaaaatat tcctttgtga aggacgtccg gggcaagggg ctgcttgtgg
     1561 gcatggaact ggattttccc ggaaaggaca tcgtcaccgc ctgcataaag gaaggcttgc
     1621 tgatcaactg caccatggac aaggtgctcc ggttcatgcc gccgctcgtc atcaccgaag
     1681 cggagatcga ccggctgatc ggggtgctgg acgggatatt cgcaaagcga tagtgaccga
     1741 aggtgcgatg gtaagaaggt acggggcaga aaaaacaaga ctcatacctt cccaccttct
     1801 taccatccta ccttcgggct tttggagact tcatgaaaaa agacctgctc gccgtttcgg
     1861 acctcgcgcc gaaagacatc gataatatcc tgaaacgcgc cgccgtcttg aaaaaaatgc
     1921 tgcgtcgggg caaggtacgg ccgaccctga agggaaagac cctcggcatg atcttcgaaa
     1981 agtcctcgac gaggacgcgg gtttccttcg aagtcggcat gtatcagctc ggcggcctgg
     2041 ccctcttcct gtcgtccgcc gacctccaga tcggccgcgg ag
//
LOCUS       DASSLD010000522         3327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_502744,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000522 DASSLD010000000
VERSION     DASSLD010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502744"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1966)
                     /locus_tag="VFK23_09440"
     CDS             complement(206..1966)
                     /locus_tag="VFK23_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04190 family B12-binding domain/radical SAM
                     domain protein"
                     /protein_id="HET7319346.1"
                     /translation="MSRPDLILLHPPSVFRFRELPLFFGPVSDVVPSSSVFEIYPIGF
                     LTLSEYLTRHGMTVRIINLALKMLRSRSFDPVRYVKNLDAAAFGIDLHWLPHVDGSLS
                     LAELVKKHHPGKPVILGGFSATYYHEEIMRDYPFVDFIVCGESTEEPLRQLLEAIRAG
                     GGYENVPNLVWRSPSGQTVINERTFKPENLDYVNFDYAHLLKMAVKYHDPTGYIPFKY
                     WHDYPVTAVFSVRGCNHDCGTCGGSLSAFNTVCRRQRPCFRSPELVVDDVRRISELTG
                     APVFVLGDLLQAGHEYSDRFLEAMKKARVRNEICIEFFRPPSADFLKKAADCIQTFNV
                     EISPESHDINVRKTFGKNYDNSALETMIETLIGVGCKRIDLFFMIGLPYQDYASVMET
                     VKYSGYLLGRYGGNGTRGRVLPEIAPLAPFIDPGSAIFENPERYGYRFFSRTLKEHRR
                     AMLMPSWKFTLNYETKWMTRDEIVRATYNGALGLLDYKERYGVVEHRSASLIRNHLER
                     AVELSGKIDDPENVDDTLREEIFSLNTLDTVCDKHELEWPVRGWKLNVPRVLRLLAEN
                     SFLLAVNKCIMPPAKSMRRR"
     gene            complement(1953..2603)
                     /locus_tag="VFK23_09445"
     CDS             complement(1953..2603)
                     /locus_tag="VFK23_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319347.1"
                     /translation="MDKADILCEKDEALLREFSRRVVRRVTGRAVPFRLPFLGDYIDA
                     NVMKEVEKDRIVIRRAAEAFHAGRGASDTDRDVLFEETKAVDRTFVQHLRIPVFSLTV
                     RYEDFADIRKKRIRLLVDEVHNLLRTWNDAATLEETLRADCPLGRFKESLTEILHLYN
                     LETRILSRSVKFLPPFHGVMAKFADVLFSTMEAESRVLVDDYADRIYGGAHAHVQA"
     gene            complement(2688..>3327)
                     /locus_tag="VFK23_09450"
     CDS             complement(2688..>3327)
                     /locus_tag="VFK23_09450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HET7319348.1"
                     /translation="FCFDQLWRRQAVARSGIRAGDRVLDVCTGTGALAILLAEKVGDK
                     GSVTGADFCAEMLNFARKKYGSRRTNMSFILSDAKRLPFGDGTFDAVTVAFGMRNIPD
                     TALALRELKRVLKPGGTFLCLELTRPHVRWFRMIYEWYLSRIMPFIAKLVLKTAAPYL
                     YLPMSINSFYPPEEFKSLVNQCGYADVTVDSLTLGIATIYRAVNPVREQARP"
BASE COUNT          673 a          918 c         1046 g          690 t
ORIGIN      
        1 cacaagatgg aggagcgaag cggcaaagaa gaaccgcagg tccatggacc ggagatgggc
       61 ataaacgttc tggagcccca ccttcctgaa tatgaccgtg aggaggaggg cgcttatgga
      121 taccttcagg gccagaaaga gaagtttctt ttttttcgag gattccaccg gacctttgaa
      181 tgcgggaaag ggtttgcggc cgttttcacc ggcggcgcat tgattttgca ggaggcatta
      241 tacacttgtt cacggccaga agaaagctgt tttcggccag gaggcgaagg acgcggggga
      301 cgttcagctt ccagcctctg acgggccatt cgagctcgtg cttgtcgcag accgtgtcca
      361 gggtgttcag gctgaatatc tcctcgcgga gggtatcgtc tacgttttca ggatcatcga
      421 tcttcccgct gagttcgacg gcccgttcca gatggttcct gatcagcgag gcgctcctgt
      481 gttcgacgac tccgtaccgt tccttgtaat caaggagccc cagcgcaccg ttataggtcg
      541 cccgcacgat ttcatcgcgc gtcatccatt tcgtttcgta attcagcgtg aacttccagc
      601 tcggcatgag catggcacgg cgatgctctt tgagggtcct ggagaaaaac cggtagccat
      661 accgctcggg gttctcgaaa atcgcactgc cgggatcgat gaagggcgca aggggggcga
      721 tttcgggaag gaccctgccc cgcgtcccgt tccccccata ccgccccagg aggtagccgc
      781 tgtattttac ggtttccatg accgatgcgt agtcctgata ggggaggccg atcatgaaaa
      841 agaggtctat ccgtttgcac ccgaccccga tcagcgtctc gatcatggtt tccagggcgc
      901 tgttgtcata atttttcccg aaggtcttgc ggacgttgat gtcgtgagac tcgggagata
      961 tttcgacgtt gaaggtctgg atgcagtcgg ccgccttttt gagaaaatcg gcggagggag
     1021 gcctgaaaaa ttcgatgcag atctcgttcc tgacgcgggc cttcttcatg gcttcaagaa
     1081 aacggtcgct gtattcgtgt cccgcctgga gcaggtctcc cagaacaaag accggggcgc
     1141 ccgtgagttc ggaaatccgc cggacgtcat cgacgacgag ttccggggac cgaaagcagg
     1201 gacgctgcct ccggcatacc gtattgaacg cggataacga cccgccgcag gtgccgcagt
     1261 cgtggttgca gcccctcacg gagaagacgg ccgtgacggg gtagtcgtgc cagtatttga
     1321 acggaatgta gcccgtgggg tcgtgatatt tgacggccat cttgagaaga tgggcatagt
     1381 caaagttcac gtaatcgagg ttctccggct tgaatgtccg ttcgttgata acggtctgtc
     1441 cggaggggct gcgccacacg agattgggca cgttctcgta tccgccgccc gcccggatcg
     1501 cctcgagcag ctgccgcagg ggctcttccg tcgattcgcc gcagacgatg aaatccacga
     1561 agggatagtc ccgcatgatc tcttcgtgat aataggtcgc gctgaagccg ccgagaatga
     1621 ccggtttgcc ggggtgatgt tttttcacga gctcggcgag agagaggctt ccgtccacgt
     1681 ggggcagcca gtggagatcg atgccgaagg cagcggcgtc caggtttttt acgtaccgca
     1741 cggggtcgaa cgaccgggac cgcagcatct tgagggccag gttgatgatc cgtaccgtca
     1801 tgccgtgccg cgtcagatac tcggacagcg tgagaaaacc gatcggatag atctcgaaga
     1861 cggaggacga cgggaccacg tcgctcaccg gcccgaaaaa gaggggcagt tcccggaacc
     1921 ggaagacgct ggggggatgg agcaggatga ggtcaggcct ggacatgggc gtgcgctccc
     1981 ccgtaaattc tgtccgcgta gtcgtcgacc aggacccggg actccgcttc catggtgctg
     2041 aacaggacat cggcgaactt cgccatgacg ccgtgaaagg gggggaggaa cttgacagag
     2101 cggctcagta tgcgggtttc aagattgtac aaatgaagga tttcggtcag tgactccttg
     2161 aaccggccga ggggacagtc tgcacgcagc gtctcctcaa gagttgccgc atcattccac
     2221 gtccggagca ggttgtggac ctcgtccacg agcagccgga tgcgcttttt cctgatgtcg
     2281 gcgaaatcct cataccgcac ggtgagggaa aaaacgggaa tccgcagatg ctgtacgaat
     2341 gtccggtcca cagccttcgt ctcctcgaac agcacgtcac ggtccgtgtc gctggcccct
     2401 cttcccgcat ggaaggcctc cgcggcgcgg cggatgacaa tgcggtcctt ctcgacttcc
     2461 ttcatgacat tggcgtcgat gtagtccccg aggaagggga gtctgaacgg gacggcccgg
     2521 cctgtgacgc gcctgacaac gcgcctgctg aattcccgga gcagggcctc gtctttttcg
     2581 cacagaatgt cggccttatc catgtttcac tccggctgag aaaacccgtt ttgcgccgtg
     2641 acggacacgg tctttgctca tcgtacggca tgaagaaccg gtttcagtca tgggcgggcc
     2701 tgttcccgaa cggggtttac cgccctgtag atggtcgcga tccccagggt gagggaatca
     2761 acggttacgt cggcatagcc gcactggttg accagcgact tgaattcttc cggagggtag
     2821 aaggaattga tcgacatggg cagatacaga tagggggccg cggtcttgag taccagtttg
     2881 gcgataaatg gcatgatccg ggacaagtac cactcgtaga tcatccggaa ccagcggacg
     2941 tgcggtcttg tcagttcgag acagaggaag gtgccgcccg gcttcagcac ccgcttgagt
     3001 tcccggaggg ccagggcggt gtcggggatg ttccgcatgc cgaaggcgac tgtgaccgca
     3061 tcgaaggtgc cgtcgccgaa gggaagcctc ttcgcatcgg acaatatgaa ggacatgttc
     3121 gtgcgtcgcg agccgtattt tttcctggcg aaattcagca tctcggcgca gaagtcggcc
     3181 ccggtgacgg accccttgtc gcctactttt tccgcaagca gtatcgcgag cgcccccgtg
     3241 ccggtacaga cgtcgagcac gcggtctccc gcgcggatcc cggaacgggc gacggcctgc
     3301 ctgcgccaga gctggtcgaa gcagaag
//
LOCUS       DASSLD010000523         2490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1346554, whole genome shotgun sequence.
ACCESSION   DASSLD010000523 DASSLD010000000
VERSION     DASSLD010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346554"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /gene="ftsH"
                     /locus_tag="VFK23_09455"
     CDS             <1..1363
                     /gene="ftsH"
                     /locus_tag="VFK23_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET7319349.1"
                     /translation="KVTFNDVAGVDEAKEELMEIIEFLRHPDKFTKLGGRIPKGVLLV
                     GPPGTGKTLLSKAVAGEAKVPFFSMSGSEFVEMFVGVGASRVRDLFNQAVQKAPCIIF
                     IDELDALGRARGVGIAGAHEEREQTLNQLLAEMDGFDPRKGVIIMAATNRPEILDPAL
                     LRPGRFDRHVLVDRPNIKDREDILRIHIRGVKLSKDVDISVLAARTPGFVGADLANIV
                     NEAALLAARKNKTEVDMTDFEAAIDRIVAGLEKKRRVMNKKEKETVAYHECGHTIVAE
                     LLPTTDPVHRVSIVQRGIAALGYTLQLPTEDRYLMTRTELLDKLCVMLGGRVAEELVF
                     HESSTGAQNDLQRAADIARSMVTTYGMTEKFGPLTFEKERRPLFLDIGMPQGAKEYSE
                     DTAREIDQEVKRLIDDAYKKVQDMLQANQDKLKVLAAVLLEKETIEGEEIRKILGLPE
                     KKG"
     gene            complement(1470..2144)
                     /locus_tag="VFK23_09460"
     CDS             complement(1470..2144)
                     /locus_tag="VFK23_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET7319350.1"
                     /translation="MKKKKSSNQPSADFKNNPFMPLKGFAPQAPPKKTAVTAPLKKVL
                     QSEDDSALFLRAVSGARRMTMEEEIDAGPLRQPTPDKQDAALPEDRELFLKAMQKIGT
                     TFTGRQPEEEELEGKQRRSPTSRMRQLKRGSIRIGDELDLHGFLKDEALARLERFIAS
                     AYQRGLDAVLVITGKGVNSPEGPVLKGAAATWLGGRGKGMVAEFSPSPRDKGGSGAFV
                     VFLKKK"
     gene            complement(2179..>2490)
                     /locus_tag="VFK23_09465"
     CDS             complement(2179..>2490)
                     /locus_tag="VFK23_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET7319351.1"
                     /translation="DGTGYPGNINLIDGKVQPGNESNDGTPRPKKGEEIPLFGRLVAL
                     ADVYDALGSRRAYKERWEEAQVLEELRACSGKHFDPEMVETLFSCLETVRNISERYPD
                     H"
BASE COUNT          483 a          781 c          754 g          472 t
ORIGIN      
        1 caaggtcacg ttcaacgacg tggccggcgt ggacgaggcc aaggaagagc tcatggagat
       61 catcgagttc ctccgccacc cggacaagtt caccaagctc ggcggccgga tccccaaggg
      121 cgtcctcctg gtcgggccgc ccggcacggg caagacgctc ctctccaagg ccgtcgccgg
      181 ggaggccaag gttccgttct tttccatgtc cggatcggaa ttcgtggaga tgttcgtggg
      241 cgtcggcgca tcccgcgtcc gcgacctgtt caatcaggcc gtgcagaagg cgccatgcat
      301 catcttcatc gacgagctcg acgccctcgg ccgcgcgcgc ggcgtaggca tcgcgggcgc
      361 gcacgaggaa cgggagcaga ccctgaacca gctcctggcc gagatggacg gcttcgaccc
      421 ccggaaaggc gtgatcatca tggcggccac gaaccggccg gagatcctcg atccggcgct
      481 cctgcggccc ggccgcttcg accggcacgt cctggtggac cggccgaaca tcaaggaccg
      541 cgaggacatc ctaaggatcc acatccgggg agtcaagctc tcgaaggacg tggacatctc
      601 cgttctcgcg gcgcgcacgc ccgggttcgt tggggccgat ctcgcgaaca tcgtgaacga
      661 ggcggccctc ctggccgcgc gcaagaacaa gaccgaagtg gacatgacgg acttcgaggc
      721 ggccatcgac cgcatagtcg ccggcctgga aaaaaagcgg cgggtcatga acaaaaagga
      781 aaaggagacc gtggcatacc acgagtgcgg gcacaccatc gtggccgaac tcctgcctac
      841 gacggacccg gtgcaccgcg tgtcgatcgt gcagcgcggc atcgccgccc tgggctacac
      901 gcttcagctc ccgaccgaag accgctacct catgaccagg accgagcttt tggacaagct
      961 ctgcgttatg ctcgggggcc gcgtggccga ggagctcgtc ttccacgagt cctcgacggg
     1021 cgcgcagaac gacctccagc gcgccgccga catcgcacgg agcatggtaa ccacgtacgg
     1081 catgaccgaa aagttcggcc cccttacctt cgagaaggag cggaggcccc ttttcctcga
     1141 tatcggcatg ccccaggggg caaaggaata cagcgaggat acggcccggg agatcgacca
     1201 ggaggtcaag cgcctgatcg acgacgcgta caagaaagtc caggacatgc tccaggcgaa
     1261 ccaggacaag ctgaaagtgc tcgctgcggt cctgctggaa aaggagacca tcgagggaga
     1321 agagatccgg aagatcctgg ggctgccgga gaagaagggg tagtataaat gtacgccgtt
     1381 ttcggttgat acgaagaagc ccggtgagtt acgatatcat ttcgagcgcg agaataatcc
     1441 ggttcatcgg tggttgatta tttcagaatt catttctttt tcaggaacac cacaaacgcg
     1501 ccgctccctc ccttgtcgcg cggcgaagga gaaaattccg ctaccatccc ttttccgcgg
     1561 ccgccaagcc acgtcgccgc cgctcccttg agcacgggcc cctcaggcga gttgaccccc
     1621 ttgcccgtga tgaccaaaac cgcgtccaaa ccccgctggt aggcgcttgc gatgaagcgc
     1681 tcgagcctgg cgagtgcctc gtctttgagg aaaccgtgca ggtccagctc gtctccgatg
     1741 cggatcgaac ctcttttcaa ctgacgcatc cggctcgtcg gcgaccggcg ctgcttccct
     1801 tcaagctcct cctcttccgg ctgccggccg gtaaacgttg ttccgatctt ctgcatggcc
     1861 ttgagaaaaa gctcgcggtc ttcggggagg gcggcatcct gtttgtccgg agtcggctga
     1921 cggagcggtc ccgcgtcgat ttcttcctcc atcgtcattc ttcgcgcccc cgataccgcc
     1981 ctcaagaaaa gcgcgctgtc gtcttcggac tgcaggacct tctttagggg cgccgtcact
     2041 gccgtttttt tcgggggagc ctgaggagcg aaacccttga gcggcatgaa gggattgttc
     2101 ttgaagtccg cggaaggttg attgcttgat ttcttttttt tcatagaagg atttggatga
     2161 ttcgaaccgg caggccggtc aatggtccgg ataccgttcg gagatgttgc ggactgtttc
     2221 gaggcaggaa aacagtgtct cgaccatctc cgggtcgaag tgctttccgg aacaggcccg
     2281 aagctcttcg aggacctgcg cctcttccca ccgctccttg tacgccctcc gcgagcccag
     2341 cgcgtcatac acatcggcga gcgcaaccag cctgccgaac agggggattt cctctccctt
     2401 cttagggcgc ggtgttccgt cgttgctctc atttccaggc tggaccttgc cgtctatgag
     2461 gttgatgttt cccggatagc cggtcccatc
//
LOCUS       DASSLD010000524         2522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1354212, whole genome shotgun sequence.
ACCESSION   DASSLD010000524 DASSLD010000000
VERSION     DASSLD010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354212"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..454)
                     /locus_tag="VFK23_09470"
     CDS             complement(53..454)
                     /locus_tag="VFK23_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7319352.1"
                     /translation="MKTAPHKKTVLIVDDHPADIQMLNEMLGDKFDTRHAASGEEAIK
                     KIHKKMPDLILLDILMPGLDGRHVLKYIRDHERQKKVAAQDRVKIVMTSVMGFPQGGM
                     KALHSQCDAYLTKPVTKENLIEVLGTLGFHL"
     gene            complement(454..1146)
                     /locus_tag="VFK23_09475"
     CDS             complement(454..1146)
                     /locus_tag="VFK23_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319353.1"
                     /translation="MSEYLYRCPFCNRDQAVTGKDRQTAFSDLTLDNADGPRRLVVKF
                     VVCPDPACRRFSLSASLHGLKQSGGRSYTGQHQKTWDLVPPSRARSFSVDIPPKVLEN
                     YQEACLTLEISPKVAAALARRCLSEMLRDFWQVQPGRLIDEIRQIKGAADPLTWEAIE
                     SVRNSGMIGARFESEGAEIVDIDPGEAQLLIGLIETLIEDWYVGREERKKRLAGIRRF
                     TGEPAAEKPPEE"
     gene            complement(1314..2396)
                     /gene="aroB"
                     /locus_tag="VFK23_09480"
     CDS             complement(1314..2396)
                     /gene="aroB"
                     /locus_tag="VFK23_09480"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET7319354.1"
                     /translation="MHTVNVDLGDRSYDIEIGADLDRAGERLQGLGLGRKMAVVTNPT
                     VKNLYGARLIDSLKSAGFLVMSIDIPDGEHSKSLDWTNAIYTALLINSFDRKSALVAL
                     GGGVIGDLTGFAAATFMRGVPFIQVPTTLLAMVDSSVGGKTGVNHPMGKNMIGAFYQP
                     KKVLMDLGALRSLPKEEFLAGMAEVIKYGVIRDGAFLDYLEKNREKILALEPEALEYI
                     VRRSCEIKAEVVAKDERESGLRAILNFGHTVGHAIERAENYTLRHGYAVAIGMVYASR
                     LAHKTGLCDASVPERVEKLVRSYGLPADLSALGRRPSVAGLIDTMQIDKKAEAGKVKL
                     VLPKKIGEVVITKEWEEERLRELLAG"
BASE COUNT          461 a          766 c          736 g          559 t
ORIGIN      
        1 gacttctgac cagcaaggtt tggattccta ggtagcttca gagcgaaacc ggctagagat
       61 gaaacccgag ggtaccaagt acctcgatca gattttcttt agtaaccggc tttgtcaggt
      121 atgcatcgca ctgagaatgc aaagccttca tgccgccctg cggaaatccc atcacgcttg
      181 tcatcacaat cttgacgcgg tcctgtgccg cgactttctt ctgccgctca tggtcgcgta
      241 tatactttaa gacgtgcctc ccgtcaaggc ccggcataag aatatcgagc agtatcagat
      301 cgggcatctt cttgtgtatc ttcttaatgg cttcttcacc gctggcggca tgacgcgtat
      361 caaacttgtc tccgagcatc tcgttgagca tctgtatatc ggcaggatgg tcgtcaacga
      421 tcaggacggt cttcttgtgt ggtgcggtct tcatcactcc tccggcggct tttccgcggc
      481 cggctcgccg gtgaaccgac ggatccccgc gaggcgcttc ttccgctcct cgcggccgac
      541 gtaccagtcc tcgatgagcg tctcgatcag gccgatcaga agctgcgcct caccagggtc
      601 gatgtctacg atctcggcgc cctcgctttc gaaccgggcc ccgatcatcc cgctgtttct
      661 cacggactcg atcgcctccc aggtgagggg gtccgcagcg cccttgatct ggcgaatctc
      721 gtcgatgagc ctgccgggct ggacctgcca gaaatcccgc agcatctccg acaggcagcg
      781 gcgcgccagc gctgcggcga ccttcgggct gatttccaga gtgaggcagg cctcctggta
      841 gttctccaga acctttggcg ggatatcgac ggagaatgag cgggcgcggg acggcgggac
      901 gaggtcccag gtcttctgat gctgcccggt gtaggagcgg cctccactct gcttcagccc
      961 gtgcagggac gcggagagcg agaacctccg gcacgcggga tcgggacaga ccacgaactt
     1021 gacgacgaga cggcgaggac cgtccgcgtt atcaagggtc agatcggaga aggccgtctg
     1081 ccggtccttc ccggtgacgg cctggtccct gttgcagaac gggcaacgat agagatattc
     1141 gctcatggtg cctcccaacc gccggaaaaa ctactggcca cagagatctc cgaggacaca
     1201 gaggggaaca ataacgagat tggatttttt catcaaattt aacagctttt ctctgtgacc
     1261 tcttctactc tgtggcgaaa aataatttta tgactttgac ctgccgtcgt gattcatcca
     1321 gcaagcagct cccggagccg ctcttcctcc cactcttttg tgatgaccac ttcgccgatc
     1381 ttcttcggaa ggacgagttt caccttgccg gcctctgctt ttttgtcgat ctgcatcgtg
     1441 tctatgagcc ctgccacgga aggcctgcgg ccaagagcag agaggtccgc gggcaggccg
     1501 taggacctta cgagcttttc cacgcgttcc ggcaccgagg cgtcgcacag gccggtcttg
     1561 tgcgcgaggc gggaggcata gaccatgccg atggcgacgg cgtagccgtg gcgcagggta
     1621 taattttccg ccctctcgat cgcgtgcccc acggtgtggc cgaagttcag gatcgcccgg
     1681 agcccggact cgcgctcgtc ctttgcgacc acctcggcct tgatctcgca ggagcggcgg
     1741 acgatgtatt cgagcgcttc gggctcgagg gcaaggatct tttcgcggtt cttctcgagg
     1801 taatcgagga acgcgccgtc tcggatcacg ccgtacttaa tcacctcggc catgcccgcg
     1861 agaaactcct ccttcgggag ggacctgagt gcgccaaggt ccatcaggac ttttttcggc
     1921 tggtagaacg cgccgatcat gttcttaccc atggggtggt tgacgccggt cttgccgccc
     1981 acgctgctgt ccaccatagc caggagcgtc gtggggacct ggatgaaggg cacgccgcgc
     2041 atgaaggtcg ccgctgcaaa gccggtaagg tcgccgatga cgcctccccc cagggccacg
     2101 agcgcggact tgcggtcgaa actgttgatg aggagcgccg tgtagatcgc gttggtccaa
     2161 tcgaggctct tggaatgttc tccgtccggg atgtcgatgg acatgaccag gaaacccgcc
     2221 gacttcaggc tgtcgatgag gcgtgctccg tacaggttct tcaccgtggg gttcgttacc
     2281 acggccatct tccggccaag gccaaggccc tggaggcgct ccccggcccg gtccaggtcc
     2341 gcgccgatct cgatgtcgta gctccggtcg ccgaggtcga cattaacagt atgcatgctt
     2401 tactctctct ttgatcgctg tgatcacgtc atcgacggac atatccgacg tgtcgatgat
     2461 cacatcggcc tgtacataaa acttctcccg gctgtctagg agttccttga tcttcgcctt
     2521 gg
//
LOCUS       DASSLD010000525         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1021054, whole genome shotgun sequence.
ACCESSION   DASSLD010000525 DASSLD010000000
VERSION     DASSLD010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021054"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="VFK23_09485"
     CDS             complement(<1..1415)
                     /locus_tag="VFK23_09485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03717.1"
                     /note="Members of this highly hydrophobic protein
                     family,regularly are found preceded by the yybP-ykoY
                     manganese riboswitch (see RF00080). A metal cation
                     transport function is proposed; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjbE family putative metal transport protein"
                     /protein_id="HET7319355.1"
                     /translation="MDSAFGTLSFDAQFLTGLFSIVIIDLILAGDNAVVIAMAVRSLP
                     RAQRTKGIIFGSAAAVLLRVVLTFFVARLLNISYVKLAGGALILWIAVKLFVEGVPEE
                     EGGREAKTLWQAVRIIVIADITMALDNMLAVGGASHGNLFLLIFGLGLSIPFIVVTSN
                     LLSLLMDKYPVIIYIGAAILGKVGAEMIITDPFTVSLLPASLRAPDMTAPDKGLQYSL
                     EALFAAGVIVTGKLWMRRTIRREEPEAVVQTPAQPQGAGKRRAILTISREFGSGGREI
                     GRAVARALGYVYIDKDTILEDIGKDGAKWEEWAKGLDEHLPTVWEKFDWSFRGFSALV
                     QRHILEHAREGGVVIMGRGGNFLLKGVPHAYRIRVTAPLEARIDRIMQREGVDQATAR
                     WLCDKTDRERSGFLHAIYGKPWDDPAEYDRVFRMTDRRVDPAVDAVITALGERERLAT
                     DEAREAVALLAAAAAVKAGIAT"
     gene            complement(1485..1646)
                     /locus_tag="VFK23_09490"
     CDS             complement(1485..1646)
                     /locus_tag="VFK23_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319356.1"
                     /translation="MAQQPKLSEEIKKMEYEPLLPVEKKLIGWSIGLGVVLLGILVWV
                     SYTFFPTGH"
     gene            complement(1725..3398)
                     /locus_tag="VFK23_09495"
     CDS             complement(1725..3398)
                     /locus_tag="VFK23_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HET7319357.1"
                     /translation="MAVWIGLFIFVVSLGAFAGKDILGWIVTTGVWTDVSKALKPFSA
                     TGMTGIISLVLTYVVLTVIMTVGAIALKADVKKFVIGFTVVFWISYICWILGSWANIA
                     VTTPPDMKKFGITWSLKLTAEAGFIIALLVGLFFGNFVPSFANAIKEAVRPELYIKTA
                     IVILGGSLGMVAAEKLGLATAVMFRGLCAIIEAYLIYWALVYFIARKYFKFSREWAAP
                     LASGISICGVSAAIATGGAIKARPVVPIMVSSLVVIFAVVELIILPFFAETFLYKEPM
                     VAGAWMGLAVKTDGAAVAAGAVAEGLILGKNAAEGIMYQPEWIKNAAAVVKVFIDIFI
                     GVWAFVLAIIWSTVIERKPGEKASAVEIWHRFPKFVIGYIVTFLIILWVCLPASKSIT
                     AMSGQVAPLTKQIAAGDAQLAALNKKVADEEAQLATLNAVKKKDKKAIASLQGQIAND
                     KAAAASLADKIGKDKAQLAPLNEQLKGPKATMGAAKAGTGEANVFRGIFFVMTFFTIG
                     LVSNFKKLWEEGIGKLAAVYVISLFGFIIWIGLIISWIFFHGVKPPLVG"
BASE COUNT          679 a         1069 c         1022 g          646 t
ORIGIN      
        1 gtggcaatgc ccgccttgac cgccgcggcg gcggccaaaa gcgcgaccgc ctcacgcgct
       61 tcgtccgtcg cgaggcgctc acgctcgccc aacgccgtga tcaccgcgtc gactgcggga
      121 tccacgcgcc ggtccgtcat ccggaagacc cggtcgtatt ccgcggggtc gtcccacggc
      181 tttccgtaaa tcgcatggag aaatccggaa cgctcccggt ccgtcttgtc gcagagccag
      241 cgagccgtcg cctggtcgac gccttcccgc tgcatgatcc ggtctatccg cgcctccagg
      301 ggcgccgtga ccctgatgcg gtacgcgtgg ggaacgccct tgagcaggaa attaccgccc
      361 ctgcccatga tgaccacgcc gccctcccgc gcgtgttcga ggatgtgccg ctgcacaaga
      421 gcggagaatc cccggaagga ccagtcgaat ttttcccaca ccgtgggcag gtgttcgtcg
      481 agcccctttg cccactcctc ccatttcgcc ccgtctttcc cgatgtcctc aaggatggtg
      541 tccttgtcga tgtagacata gcccagtgcc cgggcaacgg cccggccgat ttccctgccg
      601 ccgctcccga actcccggga aatggtcaat atggcgcgcc gcttccccgc cccctgcggc
      661 tgggccggtg tctgtacgac cgcctccggt tcttccctcc ggatggtccg gcgcatccag
      721 agcttgccgg taacgatgac tcccgccgca aaaagcgcct cgagggaata ttgaagccct
      781 ttgtccggtg cggtcatgtc cggggcgcgg agggaggcgg gcagcaggct gacggtgaag
      841 gggtccgtga tgatcatctc ggcgccgact ttgcccagga tcgctgcgcc tatgtagatg
      901 atgacggggt acttgtccat gagcagggac agaaggttgc tcgtgaccac gatgaagggg
      961 atggagaggc cgagcccgaa gatcagcagg aacagattgc cgtgggacgc cccgccgacg
     1021 gccagcatgt tgtccagggc catcgtgatg tccgctatca caatgatcct gacggcctgc
     1081 cagagggttt tggcctccct gccgccttcc tcttccggca cgccttcgac gaagagtttc
     1141 acggcgatcc acaggatcag ggcgccgccc gcaagtttca cgtagctgat gttcaggagc
     1201 cgggctacga agaacgtaag aacgacccgc agcagaaccg cggctgccga accgaagatg
     1261 atgcccttcg tacgctgggc gcggggcagg gaccgaaccg ccatggcgat gaccacggcg
     1321 ttgtctcccg ccaggatgag atcgatgatg acgatgctga agagaccggt caggaactgc
     1381 gcgtcgaagc tgagggtccc gaatgccgaa tccaaatcat gcacctccac aaaaaaaggg
     1441 cggcaggccc gttccctgcc gcccgaaaga atgccctgcg aaccctagtg tcccgtcggg
     1501 aagaacgtgt agctcaccca gacgaggatg cccaagagga cgacgcccag gccgatgctc
     1561 cacccgatga gcttcttctc cacgggaagg agcggctcgt actccatctt tttaatttct
     1621 tcggacagtt tcggttgttg cgccatgttt tctcctttag ttcagccccc tcacccctgt
     1681 ccctctccct ccgggggaga gggaatagag tgagaggatt ttcgttagcc caccagcggc
     1741 ggtttcaccc cgtggaagaa tatccatgag atgatcagcc cgatccagat gataaacccg
     1801 aagagcgaga tcacatagac cgctgcaagc ttgccgatcc cttcttccca gagcttcttg
     1861 aagttcgaca cgaggccgat tgtaaagaag gtcatgacga agaatatccc ccggaagacg
     1921 ttggcctcgc cggtaccggc cttggcggcg cccatggtcg ccttcggtcc tttgagctgt
     1981 tcgttcaggg gcgcgagctg ggccttgtcc ttcccgatct tgtcggccag cgaagccgcg
     2041 gcggccttat cgttcgcgat ctggccctgc agcgaagcga ttgccttctt gtcctttttc
     2101 ttgacggcgt tcagggtggc aagctgcgcc tcctcgtcgg cgaccttctt gttcagggcg
     2161 gcaagctggg catccccggc tgctatctgt ttcgtaaggg gggccacctg gccgctcatg
     2221 gccgttatgc tcttggatgc cggcaggcac acccacagga tgatcaggaa ggtcacgata
     2281 tatccgatga cgaatttggg gaagcggtgc cagatctcga ccgcgctcgc cttttcaccg
     2341 ggcttgcgct cgatgaccgt ggaccagata atggccagga cgaaggccca cacgccgatg
     2401 aagatgtcga tgaacacctt gaccacggcc gcggcattct tgatccattc aggctggtac
     2461 atgattcctt cggcggcatt cttgccgagg atgaggccct cggcaacggc gcccgcggcc
     2521 acagcagcgc cgtcggtctt gaccgcgaga cccatccatg cgccggcaac catcggttct
     2581 ttatagagga aggtctctgc aaagaacggc aatatgatga gctcgaccac ggcgaagatc
     2641 acgacgagcg aagagaccat aatcggcacc accggccggg ccttgatcgc tccgccggtc
     2701 gcgatggccg cggacacgcc gcagatcgag atgcccgagg cgaggggagc ggcccattca
     2761 cggctgaact tgaagtattt gcgggcgatg aagtagacga gcgcccagta gataaggtag
     2821 gcttcgatga tggcgcagag gccgcggaac atgaccgcgg tggccagtcc cagtttctcc
     2881 gcggccacca tgccgaggga gccgccgagg atgacaatgg cggtcttgat gtacaattca
     2941 ggccggacgg cctctttaat cgcgttcgca aaggaaggga cgaagttgcc gaagaacagg
     3001 cctaccagga gcgcgatgat gaaccccgct tcggccgtga gctttaacga ccaggtgatc
     3061 ccgaacttct tcatgtccgg cggagtggtc accgcgatgt ttgcccagct cccgaggatc
     3121 cagcagatgt aactgatcca gaatacgacg gtaaaaccga tgacgaactt tttgacgtct
     3181 gccttgagcg ctatcgcgcc aacggtcata atgacggtca ggacaacata cgtaagaacg
     3241 agtgagataa tgcccgtcat cccggtggcg gaaaacggct tcaatgcctt cgacacgtcg
     3301 gtccatacgc cggtggttac gatccatccc agaatgtcct tgccagcgaa ggcccccagt
     3361 gagacgacaa agataaacag cccgatccat acggccaacc agtcctcgct gagtcc
//
LOCUS       DASSLD010000526         3408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1113976, whole genome shotgun sequence.
ACCESSION   DASSLD010000526 DASSLD010000000
VERSION     DASSLD010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113976"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /gene="ssb"
                     /locus_tag="VFK23_09500"
     CDS             complement(<1..291)
                     /gene="ssb"
                     /locus_tag="VFK23_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET7319358.1"
                     /translation="MTSFNKVILLGNLTRDPEVRYTPNGIAVASFAIAVNRKYKQGDE
                     TKEEVSYIDIVVFGKQAESCGQYINKGDSVLIDGRLQQRRWETEDGQKRNKIE"
     gene            complement(404..778)
                     /gene="rpsF"
                     /locus_tag="VFK23_09505"
     CDS             complement(404..778)
                     /gene="rpsF"
                     /locus_tag="VFK23_09505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET7319359.1"
                     /translation="MPIYESIFIINANLTDEETANVIKKMQDVVAKQGGEMLKFEDWG
                     KKKLAYEIKKQKRGHYVFFQFKAAPVAVAELERTYKMTDSVIKFITVKLEKELRVKEP
                     PKPKKKDAAKAAAEGSQTQAQG"
     gene            1140..2315
                     /gene="alaC"
                     /locus_tag="VFK23_09510"
     CDS             1140..2315
                     /gene="alaC"
                     /locus_tag="VFK23_09510"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HET7319360.1"
                     /translation="MPEQFGRIKRLPPYVFAIVNTMKIEARRRGEDIIDLGMGNPDQG
                     TPQHIVDKLVEAARNPKNHRYSASKGITKLRTAVCDWYKRRYDVDLDPESETVVTIGS
                     KEGLSHLALATVEPGDVVLTTTPAYPIHPYAVIIAGGEVRSIPLRKDGDFFSDMQEAY
                     KNTWPRPKMMIINFPNNPTTAVVDEAFFQKVADFARENNIIVVHDLAYADLNFDGYRA
                     PSFLQAKGAKDVGVEFFSLTKSYNMAGWRVGFCCGNKDVVGALIKIKSYLDYGMFQPI
                     QIASIIALNGPQECVSEITEMYRCRRDVLIDGLDKAGWKIEKPKATMFVWAEIPEPFR
                     KAGSLEFSKYLIKEAGVAVSPGIGFGEYGDSHVRFALIENEHRLRQAVKGIKQAIQK"
     gene            2428..>3408
                     /locus_tag="VFK23_09515"
     CDS             2428..>3408
                     /locus_tag="VFK23_09515"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET7319361.1"
                     /translation="MTSKKSVNVGIIGFGTVGTGTARILIDNADIIRRRLGAPVILKK
                     ISDLDIKKDRGIKLGEVKLTTDAKDIIGDPDIDVVVELIGGYKPAKEFILEAMKNKKH
                     VVTANKALLAVHGEELYAAATKYGVTIGFEASVAGGIPILAAVRNGLAANNIKSIYGI
                     VNGTCNYILTLMTNAGRKFDEVLREAQAKGYAEADPTFDIEGIDSAHKLAVLTMLAYG
                     TPVKFNDIYTEGISKITPLDIDFAKELGYKIKLLAITKMVNGEVEARVHPTMLPEEYP
                     IATVDGVFNALSVVGDAVGQTMFYGRGAGDMPTGSAVVSDIIDIGRDILAG"
BASE COUNT          776 a          936 c          934 g          762 t
ORIGIN      
        1 ttcgatcttg tttcgtttct gaccgtcttc ggtttcccag cgccgctgct ggagcctgcc
       61 gtcgatcagc acagagtctc ccttgttgat gtactggccg caggattccg cctgtttccc
      121 gaagacgacg atatcgatgt agctgacttc ttccttggtc tcgtcaccct gcttgtactt
      181 gcggttgacc gctatggcaa agcttgcaac agcgatgccg ttcggcgtat agcggacctc
      241 ggggtcacgg gtcagattgc ccagcagaat taccttatta aagctcgtca tcgcgttacc
      301 tcattgggta cggatatttg ataccggatt tcggatgctc gattcctgat tttatccggt
      361 atcaagcatc gtttttttcc cagcatccgg tatcggttta gatctagcct tgcgcctgtg
      421 tctgactgcc ctctgcagcc gcctttgccg cgtccttttt cttcggtttc ggcggctcct
      481 tgacgcgcag ttctttctca agtttgacgg taatgaactt gatgaccgaa tccgtcattt
      541 tgtaggtgcg ttcgagttcg gctacggcaa caggggcagc cttgaactgg aaaaagacat
      601 agtggccgcg tttctgcttc ttgatttcgt aggccagttt tttcttgccc cagtcctcga
      661 acttgagcat ctcaccgcct tgtttcgcaa cgacatcctg cattttcttt atgacattgg
      721 cggtctcttc atcagtgagg ttggcgttta tgatgaagat ggattcgtat atcggcatgg
      781 agtgttccct ccttgtgggt tatagccctt ccgacgataa agccccccct cttcctttcc
      841 cccccctcca gggaagaggg cagggatgaa ggagtttaca tatatcggaa gagcaaggag
      901 taatcccgcg tattgacgcc atcgggaatc gaccttactc gtttttggaa agctgaacat
      961 tatcacaggc aaattgaaaa atcaagactt ttctcccgtg taaccccatt ccgtccatgg
     1021 catagcatgg ctggacaagt attttttatt gacaaaacca gcttactgtt ctattatcaa
     1081 ttttactttt aaattgcatg gttagcagca taatctcttt gaaaaagaaa gggttatcaa
     1141 tgcccgagca gttcggcagg atcaagagac tgccgcccta tgttttcgcg attgtcaaca
     1201 cgatgaagat cgaagcgcgc cggcgcggcg aggacataat cgatctcggg atgggcaacc
     1261 ctgatcaggg aacgccgcag cacatcgtgg acaagctggt cgaggcagca cgcaatccga
     1321 agaaccaccg ctattccgcg tcgaagggca tcaccaaact gcgaactgcc gtctgcgact
     1381 ggtataagcg gcgctatgac gttgacctcg accccgaatc cgagaccgtg gtgaccatcg
     1441 gctccaagga gggcctgtcc catctcgcgc ttgccacggt cgaaccggga gacgtagtgc
     1501 tcaccaccac gccggcctat cccattcatc cctatgccgt tatcattgcg ggcggcgagg
     1561 tgcgctccat tcccctccgg aaggacggcg acttcttctc cgacatgcag gaggcctaca
     1621 agaacacctg gccgcggccc aagatgatga tcatcaattt cccgaacaac cccaccaccg
     1681 ccgtcgtgga cgaggcattc tttcagaagg tcgcggactt cgcccgagaa aacaacatca
     1741 tcgtggtcca tgatcttgcc tacgcggacc tgaactttga cgggtaccgg gcgccgagct
     1801 ttctccaggc caagggcgcc aaggacgtgg gggttgagtt tttcagcctt acgaagagtt
     1861 ataatatggc gggatggcgc gtgggattct gctgcggcaa caaggacgtg gtaggcgcgc
     1921 ttatcaagat caagagctac ctggactacg gcatgttcca gcccatccag atcgcatcga
     1981 tcatcgcgct gaacggaccg caggagtgtg tgagcgagat aacggagatg tatcggtgcc
     2041 ggcgcgatgt gctcatcgac gggctggaca aggcgggctg gaaaatcgaa aaaccgaagg
     2101 ccaccatgtt cgtgtgggcc gagatcccgg aaccgttcag gaaggcgggg tcactcgagt
     2161 tttccaagta tctcatcaag gaagcggggg tcgccgtctc gccgggaatc ggcttcggcg
     2221 aatatggcga cagccacgta cggttcgcgc tcatcgaaaa cgaacatcgc ctgcggcagg
     2281 cggtaaaagg gatcaagcag gcaattcaaa aataaattca agtttcgaaa tcctcaatcc
     2341 tcaacaatag cgaagttgat cggaatacga ctatttgaat tcattgttga gtttggaatt
     2401 taggacctat acaacaaggc ggatgaaatg acatcaaaga aatcggtcaa cgtcggcatc
     2461 atcggcttcg gcaccgtggg cacgggcact gcgcgcatcc tgatcgacaa cgcggacatc
     2521 atccggcgca ggctcggcgc tcccgtcatt ctaaagaaga tatcggacct cgacatcaag
     2581 aaggaccgcg gcatcaaact gggagaggtc aagctcacga ccgacgcgaa ggacatcatc
     2641 ggcgacccgg acattgacgt cgtggtcgaa ctcatcggcg ggtacaagcc ggccaaagag
     2701 ttcatccttg aggccatgaa aaacaagaag cacgttgtga ccgccaacaa ggctctcctg
     2761 gccgtgcatg gcgaggaact ttacgccgcc gcgacaaagt acggcgtaac catcgggttc
     2821 gaggcgtcgg tggcgggcgg catcccgatc ctcgctgcgg tcaggaacgg gcttgccgct
     2881 aataacatca agtccattta cggcattgtg aacggcacct gcaactacat tctgaccctc
     2941 atgaccaacg ccggaaggaa gttcgacgag gtgctccgcg aggcccaggc aaagggctac
     3001 gctgaggcgg accccacctt cgatatcgag gggatagact ccgctcacaa actcgcggtc
     3061 ctgacgatgc ttgcctacgg aacgccggtg aagttcaatg atatctacac cgaagggatc
     3121 tcgaagataa cgccgctcga catcgacttt gcaaaagaac tagggtataa gatcaagctg
     3181 cttgcgatca ccaagatggt gaacggggag gtcgaggcgc gcgtgcatcc gaccatgctt
     3241 ccggaggaat atcccatcgc caccgtggac ggcgttttta acgccctctc ggtcgttggc
     3301 gacgcggtcg gccagacgat gttttacggc aggggggcgg gggacatgcc gaccggaagc
     3361 gccgtcgtct ccgacatcat cgacatcggc agggacatcc tggcaggc
//
LOCUS       DASSLD010000527         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_566621,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000527 DASSLD010000000
VERSION     DASSLD010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566621"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFK23_09520"
     CDS             <1..612
                     /locus_tag="VFK23_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HET7319362.1"
                     /translation="VEYCMMVDIDQEVVEKSKELLPEWHQGAFDDPRTDLRFMDARKY
                     LEETSDSYDAIIIDISEPVEEGPAYLLFTREFYEIVKERLSPDGTISLQAGTTALHQL
                     LNFTAVDKTLRSVFPVVAPYNVSIPSFGLPWGFTLASKNLDPRTFTPEHVNDLIRKRI
                     KGENRYYDGITNQGMYLLPKHIRKAMAEETRIIEDNAPLFTYH"
     gene            619..2103
                     /locus_tag="VFK23_09525"
     CDS             619..2103
                     /locus_tag="VFK23_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7319363.1"
                     /translation="MSHPPNRIEDEQFRTPLFDAMVNLAESRKVSFHTPGHKSGKGIS
                     TRFRKFVGPRIFSIDLTTLDEVDSLQNPTGVIKEAQELAARAAGADRSYFLVNGTTVG
                     NHAMVASSTGPGDKVLIARNCHRSVLTGLIMSGAQPVFFQPAFDRDLKLTLNVPFETA
                     KAAIDANPGARALLLTSPNYYGLCADIERIIPYARDKGLLVFVDEAHGPHLKFNPKLP
                     TCALEAGADLCVQSTHKIVGGMTQASMLHARAGRINVDDVTNTLKLLQTTSPSYILMA
                     SLDLARMQMATEGKKLLNRTIKLAEDARAEINKIPGIACFGKDRAKQAGMADMDVTKL
                     TITVSDLGLSGYHVSQMLNLRFGIQVEMADPFHVLVIVSIGDRQDDLNRLVEALKVIS
                     AESGLQGALLPLDRVHPPVLDNRFAMTPRDAFYSDSELVDVRDSAGRVSTEIVTVYPP
                     GIPLLVPGEEISRDALDYLQNMAGLGAIIDGLNENNTLVRVAKS"
     gene            2225..>2564
                     /locus_tag="VFK23_09530"
     CDS             2225..>2564
                     /locus_tag="VFK23_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319364.1"
                     /translation="MTTVFNKIRVKISLFVVVLLFLTTLVFYLTTLKIMEVHITNEVV
                     KKSEAMTKSIAVSAGYSILSKDLLGLDNMVFKMQQSNPDVEFIAVIGNDRKIIAHSVI
                     GEVGKKIDDVP"
BASE COUNT          556 a          830 c          713 g          465 t
ORIGIN      
        1 gtcgaatact gcatgatggt ggacatcgac caggaagtgg tggaaaaatc aaaggaactc
       61 ctgcccgagt ggcatcaggg cgccttcgac gacccccgga ccgacctcag gttcatggac
      121 gcccgaaaat atctggaaga aacctccgac agctacgacg cgatcattat cgatatatcc
      181 gagccagtcg aagaagggcc ggcctatctg ctctttaccc gcgagttcta cgagatcgtc
      241 aaggagcgtc tctccccgga cggcacgatc tcgctccagg ccgggaccac ggccctgcac
      301 cagctcctca atttcacggc tgtggacaag accctgagat cggtattccc ggtggttgcg
      361 ccctacaacg tgtccatccc gtccttcggc cttccctggg gcttcaccct tgcctcgaag
      421 aatctcgatc cccggacgtt caccccggag cacgtgaacg acttgatcag gaagcggatc
      481 aagggagaga accgctacta cgacggcatc acgaaccagg gcatgtacct gctgcccaag
      541 cacatccgca aggccatggc cgaagagacc aggatcatag aggacaatgc gcccctcttc
      601 acgtaccatt agaaggcaat gagccaccct cccaaccgaa tagaggacga gcagtttcgc
      661 acccccctgt tcgacgcaat ggtcaacctt gccgaaagcc gcaaggtctc cttccatacc
      721 cccggacata agagcggcaa aggcatatcc acccggttcc gcaagttcgt gggtccgcgc
      781 atcttttcca tcgacctgac gaccctcgat gaagtggatt ccctccagaa ccctacgggc
      841 gtgatcaagg aggcccagga actggcagcc agggcggccg gggccgatcg ctcctacttc
      901 ctggtgaacg gcacgaccgt gggaaaccac gccatggtgg cctcctcgac ggggccgggg
      961 gacaaggtcc tgattgcgag aaactgccat cgttcggtgt tgaccggcct catcatgagc
     1021 ggtgcccagc cggtcttttt ccagccggcc ttcgacaggg acctcaagct caccctgaac
     1081 gtcccgttcg agacggcgaa ggccgccatc gacgcaaacc ccggagcgcg ggcgcttctt
     1141 ctcacgagcc cgaattatta cggactctgc gcggacatcg aacggatcat cccctatgcc
     1201 cgcgacaagg gccttttggt gttcgtggac gaggcgcacg gcccgcacct caagttcaat
     1261 cccaagctcc ccacatgcgc cctcgaggcc ggcgcggacc tgtgcgtgca gtccacccac
     1321 aagatcgtgg gcggcatgac ccaggcgtcc atgctccatg caagggcggg caggatcaat
     1381 gttgacgacg tgacgaacac cctgaagctc ctccagacca cgagcccctc ctacatcctt
     1441 atggcgtctc tggacctggc caggatgcag atggccacgg agggcaaaaa gctcctgaac
     1501 aggacgatca agctggccga ggacgcgcgc gccgagatca acaagatccc cggcatcgcc
     1561 tgtttcggca aggaccgggc aaagcaggcc ggcatggcgg acatggacgt gaccaaactc
     1621 accatcacgg tgtcggatct cgggctttcc ggctaccacg tctcccagat gctgaacctc
     1681 cgcttcggca tccaggtcga gatggcggac cccttccatg tgctcgtgat cgtctcgatc
     1741 ggcgaccgcc aggacgacct gaaccgcctg gtggaggcgc tcaaggtgat ctccgcagag
     1801 agcgggctcc agggggcgct tctgccgctt gaccgggtcc atcccccggt cctggacaac
     1861 cgcttcgcca tgaccccgcg cgacgccttc tattcggaca gcgagctggt cgatgttcgc
     1921 gattccgcgg gaagggtctc aaccgaaatc gtgaccgtct atcccccggg aattccgctc
     1981 ctggtccccg gggaggagat ctctcgggac gccctcgact acctgcagaa catggccggt
     2041 ctgggcgcca tcatcgacgg cctgaacgag aacaacaccc tcgttcgcgt ggcaaaaagc
     2101 tgagctgccg cttcttctgc tgtcatgaga cgctccgaag aacggcactt gaattttttt
     2161 cgcttctcgg atacactaag ggggacgttc ggcgggcagg ccccgacgcc gaaccctgac
     2221 gctcatgacc accgtgttca ataaaatcag agtcaagatc tccctgttcg tcgtcgtgct
     2281 gctgtttctc acgaccctcg ttttttacct caccacgctg aagatcatgg aagtccacat
     2341 cacgaacgag gtcgtcaaga aatccgaggc gatgaccaaa agcatcgcgg tctcggcggg
     2401 gtacagcatt ctctcgaaag atctgctggg cctcgacaac atggtcttca agatgcagca
     2461 gtccaatccg gacgtggaat tcatcgccgt tatcggaaac gacaggaaga tcatcgctca
     2521 tagcgtcatc ggcgaagtag ggaaaaaaat cgatgacgtc ccgg
//
LOCUS       DASSLD010000528         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_564487,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000528 DASSLD010000000
VERSION     DASSLD010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564487"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFK23_09535"
     CDS             <1..588
                     /locus_tag="VFK23_09535"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET7319365.1"
                     /translation="VPLRIKQEKGGDKIFDTDEHPRFGTTCDALAKLRPAFRDEGTVT
                     AGNASGINDGAAAVLVVSGEKMKEMKPAWAFKIRGSSTAAIDPAFMGLGPIGACKNVI
                     SRSGITLKDLDLIEINEAFAAQSIQVHRELGWDMNKVNVLGGAIALGHPVGASGTRIL
                     VTLLYEMVRRNANLGLASLCIGGGQGIAVIVERVR"
     gene            complement(926..>2029)
                     /locus_tag="VFK23_09540"
     CDS             complement(926..>2029)
                     /locus_tag="VFK23_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7319366.1"
                     /translation="QGPDTNELYEAASAFEIKMKGLPELQDVTSDLQIKNPQVNLVID
                     RDKAAALGVSAHQIESALGMAYSSLQVSTILAPTNQYQVIMELQPQYQADASSLTMLY
                     VRSSSGKLVPLNVVTKHSVGTGPLLVNHQGQLPAVTISFNLKPGVALSKAVDVIGKVS
                     HETLPPSITTSFQGTAQAFQSSTKGLFLLLIMAVLVIYIVLGILYESFIHPLTILSGL
                     PAAGVGALLTLLIFGQELNIYGFVGIIMLIGIVKKNAIMMIDFALEAERKEGKSPQDA
                     IYEGAVIRFRPIMMTTMSALMGALPIAVGLGAGAESRRPLGLAVVGGLIFSQLLTLYI
                     TPVIYTYLDAFQHRFGRLLLFRARGRAAGAVRD"
BASE COUNT          429 a          580 c          617 g          403 t
ORIGIN      
        1 gtcccgctgc ggatcaagca ggaaaagggc ggggacaaga tcttcgatac cgacgagcac
       61 ccccgcttcg gcacgacctg cgacgcactc gcgaaactga ggcctgcgtt cagagatgaa
      121 ggaacggtga cggccggaaa cgcttcgggg atcaacgacg gggccgcagc cgtgctcgtc
      181 gtgtccggag aaaaaatgaa ggaaatgaag ccggcctggg cgttcaagat caggggctcg
      241 tcaacagcgg ccatcgatcc ggcgttcatg gggctcggcc ccatcggcgc ctgcaagaac
      301 gttatttccc gctcggggat aactttgaaa gacctcgacc tcatcgaaat caacgaggcc
      361 ttcgccgctc agtcgatcca ggtgcaccgg gaactgggct gggatatgaa caaggtgaac
      421 gtgctgggcg gcgccatcgc gctggggcac ccggtggggg cgagcggaac gaggattctg
      481 gtcacgcttc tctacgagat ggtgaggagg aacgcgaacc tgggcttggc ctcactctgc
      541 atcggcggcg gccagggcat tgcggtgatc gtggaacggg tgaggtaaca gcgctatcct
      601 gcttcaactt ccttcccaaa atcagggcgt ggaattccga accgtttgaa tccgctccct
      661 catctctcgc attgaacgcg ccggtcacgt gccggctttc ggcacggcgc ggtggaccgg
      721 ttcgttatga ttccgtagtt ttcgatatgt ctcggcttta taaatggccc aatgaattga
      781 gtctctattc gccggaaaca tcacttggta aattattgat cgccgaccaa taccgttctt
      841 taagataccg gttatcagca atatgagctt gaagaacgaa agcggcggcc aaggcatcct
      901 ttccccggcc ggaccaggct gaacatcagt cccgcacggc acccgcagcc cgccccctcg
      961 ccctgaacag caacagtctc ccgaacctgt gctgaaaggc atcaagatac gtgtagatga
     1021 cgggcgtgat atagagggtc agcagctggg aaaagatgag gcctcccacc accgcaagcc
     1081 ccagaggcct gcgcgattcc gccccggcgc cgagaccgac cgcgatggga agggcgccca
     1141 tgagagcgga catcgtcgtc atcatgatcg gcctgaacct gatgacggcg ccttcgtaaa
     1201 ttgcgtcctg cgggctcttc ccttccttcc gttcggcttc gagggcgaaa tcgatcatca
     1261 tgatcgcatt ctttttgacg atcccgatga gcatgatgat gcccacgaat ccgtagatgt
     1321 tcagctcctg accgaagatc aagagcgtca ggagcgcacc cacccccgcc gccggaagac
     1381 cggaaagtat ggtaagggga tggatgaagc tttcgtagag aatgccgaga acgatataga
     1441 taacgagaac ggccatgatg agcaggagaa acagtccctt cgtcgacgac tggaaggcct
     1501 gcgccgtgcc ctggaaactg gtcgttatcg agggcggaag cgtctcgtgt gaaactttgc
     1561 cgatgacatc caccgccttg ctgagcgcga ccccgggctt caggttgaat gaaatcgtga
     1621 ccgcaggcag ctgtccctgg tgattgacca gcagcgggcc ggttccgacc gagtgtttcg
     1681 tgacgacgtt cagcggtacg agtttgcccg acgaggagcg gacatagagc atcgtgagcg
     1741 aggacgcatc agcctgatac tggggctgca gttccatgat cacctggtac tggttggtcg
     1801 gcgccaggat ggtcgacacc tggagcgagc tgtacgccat gccgagggcg ctttctatct
     1861 ggtgggcgct caccccgagc gccgcggcct tgtcccggtc gatgaccagg tttacctggg
     1921 gattttttat ctgcaggtcg ctcgtcacgt cctgcagctc cgggagcccc ttcattttta
     1981 tttcgaacgc ggaagccgct tcatagagtt cattcgtgtc gggtccctg
//
LOCUS       DASSLD010000529         3027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_896598,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000529 DASSLD010000000
VERSION     DASSLD010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896598"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VFK23_09545"
     CDS             <1..725
                     /locus_tag="VFK23_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET7319367.1"
                     /translation="VERGLLREAEARSLSDAEAVNLIFRPGFSTASRVGRVSGRGVGM
                     DVVSTHLSRINGRIELRTGAGVGTKFVIHLPLTLAIAQALIVKLGDQEMAVPINFVEE
                     TTSFSGKDIQEAGGGETVNLRGTLLKLSRLNDLLGVGRLLIKDDACRHAALILGMAGK
                     RIALLVEEITGREEIVVRPLGEYLKSVRLFSGASISGEGGVRLIINVFPLFDEEGAAG
                     YRAFGCGAEETSAWSSPSRNRI"
     gene            895..2199
                     /locus_tag="VFK23_09550"
     CDS             895..2199
                     /locus_tag="VFK23_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7319368.1"
                     /translation="MTVKILLADKSITIQKVVEMLFSGRDYEVLCVSDGESALSEASR
                     TIPDVVLADVDLPKMDGYAFSARLRQTLQLAKIPVILMMSRDDVYDSAKGKAAGITDN
                     IAKPFESQDLIAKVKRAVSPVSSPSAEPARPASEQKPAAPVSHPAAAAPPPPQSAVAT
                     PKQAAPADIFEIIQEAPTASEVRQAAGLPGGEEETVYEVEPEVEEVEEPMPREVAKAL
                     PVGEKAVEEMRAGLGLEEAEEERAEAVPFDAFETVVDAEPTTAATEAAAAARPETAPA
                     AAAPPPLPEGEIRTMVEEAVAKLAKETFKTMPLPQPSAISESDLWGIAEEAVSRMAKE
                     VFAQMPPARTAQPPALPESELRRMAEEAIARMAEDAFKDMPPPPLPKISDETVRRGIE
                     DAVMKVAREIAKEVIERVAWDVIPPLAEQMIREEIERLKAME"
     gene            2402..>3027
                     /locus_tag="VFK23_09555"
     CDS             2402..>3027
                     /locus_tag="VFK23_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET7319369.1"
                     /translation="MSSQELPKTYDPKAVEQKWYAFWLEKNYFHADENAGGPSFSIVI
                     PPPNVTGSLHIGHALNSTLQDILVRWMRMSGRNTLWVPGTDHVGIATQNVVERQLAKD
                     HIDRHALGREKFVEKVWEWKKEYGGRIINQLKRMGASCDWQRERFTMDEGLSRAVREV
                     FVTLYEEGLIYRGERLINWCPRCHTALSDIEVEHEDEKGKLYHIAYPLS"
BASE COUNT          642 a          886 c          977 g          522 t
ORIGIN      
        1 cggtggagcg gggccttctc cgggaggccg aggcccggtc tctctccgac gccgaggccg
       61 ttaatctcat tttccgcccc ggtttttcaa cggcctcccg cgtgggcagg gtgtccggcc
      121 gcggcgtggg catggatgtc gtctcgacgc acctctcgcg gatcaacggc aggatcgagc
      181 tcaggaccgg ggcgggcgtc ggcacgaagt tcgtgataca ccttccgctc accctcgcga
      241 tagcccaggc gctgattgtc aagctcggcg accaggagat ggccgttccc ataaatttcg
      301 tggaagagac gacaagtttc agcgggaagg acatccagga ggcgggtggc ggggagacgg
      361 tgaacctccg aggcacgctc ctgaagcttt ccaggctgaa cgatcttttg ggggtcggca
      421 ggttactgat aaaagacgat gcctgccgcc acgccgcgct gatccttggc atggccggga
      481 agcggatcgc gctcctcgtc gaggagatca cgggccgcga ggagatcgtg gtcagacccc
      541 ttggcgaata cctgaagagc gtcaggctct tctcgggcgc gtcgatctcc ggtgaaggcg
      601 gtgtccggct catcatcaat gttttcccgc tcttcgacga ggagggcgcg gccgggtacc
      661 gggccttcgg ctgcggggcg gaagaaacgt ccgcctggtc gagcccttcg agaaacagga
      721 tatgaccgag gcgctgaaac ggctgctcgc ggttgccgcc ccggcagccc gggcctaagc
      781 gttaaaacta tggacgaagg gactcgattt ctgatattat cagtgggcga agaagttacg
      841 ccgtattcga tgacgatcag gaaataacaa ttctcgactt tgaaaggttg ctccatgacg
      901 gttaaaatac tgctcgctga caaaagcatc accatccaga aagtcgtgga aatgcttttt
      961 tccggccgcg actatgaagt gctgtgcgtg agcgacggcg agagcgccct gtcggaggcg
     1021 tcccgcacga tccccgatgt cgtgctcgcc gacgtcgacc tgccgaagat ggacggctat
     1081 gccttttccg cccggctcag acagacgctg cagctcgcaa agatccccgt tatcctcatg
     1141 atgagcaggg acgatgtcta cgactccgca aaaggcaagg cagcggggat cacggacaat
     1201 atcgccaagc ctttcgaatc gcaggatctg atcgccaagg tcaagagggc cgtttccccg
     1261 gtttcatcgc cgagcgcaga gcccgcccgt cccgcgtccg aacagaaacc ggcagcgccg
     1321 gtttctcatc ccgccgcggc cgcgcctcct ccccctcagt ccgcggttgc cacgcccaag
     1381 caggccgcgc ccgccgatat ttttgagatc atccaggagg cgccgaccgc ttcggaggtc
     1441 aggcaggcgg cgggtctgcc cggaggcgag gaagagaccg tttacgaggt tgagcccgag
     1501 gttgaggagg tcgaggaacc gatgccccgg gaggtagcaa aggccctgcc cgtcggtgaa
     1561 aaggccgtgg aggagatgcg ggcgggactg gggttggaag aggcggagga ggagcgggcc
     1621 gaggccgtgc ccttcgatgc ctttgaaacc gtcgtggatg cggaaccgac gacggcggcg
     1681 acggaggccg cggcagctgc ccgtcccgag accgcgcctg ccgcagccgc cccgccgccg
     1741 ctgccggaag gcgagatccg cacaatggtc gaagaggctg ttgcgaagct ggcgaaggaa
     1801 acgttcaaaa ccatgccgct tccgcagccg tcggcgatat cggagagcga tctctggggc
     1861 atcgcggaag aggccgtttc aagaatggcc aaggaagtat tcgcccagat gccgccggcc
     1921 cggacagcgc agccgcccgc gctgcccgag agcgaactcc ggcggatggc agaagaggcc
     1981 attgcgcgga tggccgaaga tgcgttcaag gacatgcccc ctccgcccct cccgaagata
     2041 tcggacgaaa ccgtgagacg ggggatcgag gacgccgtca tgaaggtcgc ccgggagatc
     2101 gcaaaggaag tgatcgaacg ggtggcctgg gacgtgatcc ccccgcttgc ggagcagatg
     2161 atcagggagg agatcgaaag attgaaggcg atggaatagc agttcggagt gcggagtgcg
     2221 gaatgaaaca aagaggggac ttgctcccct ctttttatta cttcaaaaaa cttttccgtg
     2281 attcccacga atacgggaat ccattttgaa gtacgcccga aaagccaatg gatacctgct
     2341 ttcgcaggta tgacaaagta aaattattac gagcgggaac acctgaagga gatcatcgtc
     2401 gatgtcatcg caggaattgc ccaagaccta tgacccgaaa gccgtggagc agaagtggta
     2461 cgccttctgg ctcgaaaaaa actattttca cgccgacgag aacgccggag gcccgtcatt
     2521 ttcaatcgtg ataccgccgc ccaacgtgac cggttcgctc cacatcggcc acgcgctgaa
     2581 ctccacgctc caggacatac tcgttcgctg gatgcgcatg tccggcagga acaccctctg
     2641 ggtgccgggc acggaccacg tgggcatcgc cacgcagaac gtggtggaac gccagcttgc
     2701 gaaggaccac atcgaccgcc atgccctggg gcgggagaag ttcgtcgaga aggtctggga
     2761 gtggaagaag gaatatggcg gcaggatcat caatcagctc aagcgcatgg gggcgtcctg
     2821 cgactggcag cgtgaacgct tcacaatgga cgaagggctc tcgcgcgccg tgcgcgaggt
     2881 ctttgtcacc ctgtacgagg aagggctcat ctaccgcggc gagcgcttga tcaactggtg
     2941 cccccgctgc cataccgcgc tctccgacat tgaagtcgag cacgaggacg agaagggaaa
     3001 gctctatcat atagcctatc ccctctc
//
LOCUS       DASSLD010000530         2770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1445339, whole genome shotgun sequence.
ACCESSION   DASSLD010000530 DASSLD010000000
VERSION     DASSLD010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445339"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFK23_09560"
     CDS             <1..392
                     /locus_tag="VFK23_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP/ATP-dependent (S)-NAD(P)H-hydrate
                     dehydratase"
                     /protein_id="HET7319370.1"
                     /translation="MGRLVGMASSDVQKDRLGVAARFAKDHKVILVLKGAGTIVALPD
                     GQLFINGSGNPGMATGGTGDVLTGLIGGFLAQGHPAGQAACLGVYLHGLAGDLAAKEK
                     GEAAMIAGDLIGKIPEAMKLVEDKVEV"
     gene            498..959
                     /locus_tag="VFK23_09565"
     CDS             498..959
                     /locus_tag="VFK23_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7319371.1"
                     /translation="MLTAKDIMTKNVITVKPDTSIEELASLLVENRISGAPVVDDSGN
                     LYGIVTENDLISRDKRLHIPTVVSFLDAAIYIESSKKFEQEVKRLTATKVGEICTRKV
                     VTVAEDASITDIATIMAEKKVYLLPVVRSGKVVGIVGKRDMVKAAALLRKE"
     gene            956..1429
                     /gene="tsaE"
                     /locus_tag="VFK23_09570"
     CDS             956..1429
                     /gene="tsaE"
                     /locus_tag="VFK23_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET7319372.1"
                     /translation="MMFSVITATPEQTWRIGELLGRGLGPGDVVCLYGDLGSGKTNFS
                     YGIALGLEVDARYITSPTFAFVNEYTGRTQLYHIDLYRIKDPEELENIGFEHYLGGDG
                     VTVIEWADRAEETLPAARLSVYLTHVDDRSREIGFLAEGERYKNLLEALRRELKL"
     gene            1551..2402
                     /locus_tag="VFK23_09575"
     CDS             1551..2402
                     /locus_tag="VFK23_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET7319373.1"
                     /translation="MKNIGIIVKDIPEARKAAKKLTAWLERRGKKVYIDADAAATVRL
                     RGYERSEIPALVDMLIVLGGDGTLLSAARHVADAHTNVPIFGVNLGSLGFMAEVSLDE
                     LYDNLEKAMAGKLETEDRMMLSAAVVREGRRLARYRVLNDVVINKGALARMMELRVSV
                     SDGPLTTLRADGLIVATPTGSTAYSLSAGGPIIHPTVHCFVVTPICPHTLSNRPIALP
                     DSVVVEVCLTSPSEDVSITLDGQIGFPLVQHDVVEIKKSRYRMKLIKHPVKSYYEILR
                     TKLKWGN"
     gene            2463..>2770
                     /locus_tag="VFK23_09580"
     CDS             2463..>2770
                     /locus_tag="VFK23_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319374.1"
                     /translation="MDEIRQEFLDIVTRVRAHLEYQKALGVRHIETAPAAAVRTPLQQ
                     ADGRAPADADGRTSATLKGPADGLAAVGGELGDCTRCKLHKGRSNIVFGEGNPKAVLV
                     "
BASE COUNT          632 a          765 c          861 g          512 t
ORIGIN      
        1 agatgggaag gctcgtaggt atggcatcgt ccgacgtgca gaaggaccgt ctcggggtcg
       61 cagcgcggtt cgcgaaggac cacaaggtca tactggtcct caagggcgcg gggaccatag
      121 tggcgctacc tgacggacag ctgttcatca acggatccgg caaccctggc atggccacgg
      181 gtgggaccgg cgacgtgctg acgggcttga tcggcgggtt cctggcgcag ggccatcccg
      241 ccggacaggc ggcctgcctc ggcgtgtatc tccatggttt agccggcgat ctcgcggcga
      301 aggaaaaggg cgaggcggca atgatagccg gggacctgat cgggaagata ccggaggcca
      361 tgaagctggt tgaagataag gttgaagttt aaattttggc ttgataataa ggtttagttt
      421 taatttatta attcattgat atataacggt ttattatttt gtcaattcgt cgaccatgac
      481 cttcctaagg ggtgcccatg cttaccgcga aagacatcat gaccaaaaac gtcatcaccg
      541 tgaaacccga cacctctatc gaggaactgg cctccctcct ggtcgaaaac cggatcagcg
      601 gggcgccggt agtggacgat tcaggcaatc tctacggtat cgtgacggag aatgacctga
      661 tcagcaggga caagcggctc catataccca cggtggtcag cttcctcgac gcggccattt
      721 acatcgagag ctcgaagaaa ttcgagcagg aagtgaagcg tctgaccgca acgaaggtcg
      781 gcgagatctg cacgcgcaag gtcgtcacgg tcgcggaaga cgcttcgatt acggacatcg
      841 ccacgatcat ggctgagaag aaggtttatc tgctcccggt cgtcaggagc gggaaagtcg
      901 tgggtatcgt tggaaagcgg gacatggtga aggcggcggc gctgctgcgg aaggaatgat
      961 gttcagcgtg atcaccgcaa cgcccgaaca gacctggagg atcggcgaac tgctcggccg
     1021 cgggctcggt cccggagacg tcgtctgtct ctacggcgac ctgggctcgg gcaaaacgaa
     1081 cttctcctac ggcatcgcac tgggcctcga agtggatgca cgatacatca cgagccccac
     1141 gttcgccttc gtgaacgaat acacgggcag gacgcagcta tatcacatcg acctctaccg
     1201 catcaaggac cccgaagaac tggaaaatat cgggtttgaa cattacctcg gcggcgatgg
     1261 cgtgaccgtg atcgagtggg ccgaccgggc ggaggagacc ctgcccgcgg cgcgcctgag
     1321 cgtgtatctt acccatgtcg acgacagaag cagggagatc ggcttcctcg cggaagggga
     1381 gcgatataag aacctgctgg aagcgctgcg gcgggagctg aagttgtaaa tcaactcggt
     1441 cgataacaat ctaccgccga gacgcagaga cgcagagaaa gatttagaaa aattgtacga
     1501 tttcctccgc gtcctccgcg actccgcggt agaagacgac tttatcaatc atgaaaaaca
     1561 tcggcatcat cgtaaaagac atccctgagg cgcgcaaggc tgctaaaaag ctcacggcct
     1621 ggctcgaacg gcgcggcaag aaggtctata ttgacgcaga tgccgccgcg actgtcaggt
     1681 tgcgtggata cgaacggtcg gagatcccgg cgctcgtgga catgctgatt gtcctgggcg
     1741 gagacgggac gctcctgtcc gccgcgcggc acgtggccga cgcgcacacg aacgtcccga
     1801 ttttcggcgt caacctgggg agcctcgggt tcatggccga ggtgtcgctc gacgagctct
     1861 acgacaacct cgagaaggcg atggccggaa aactggagac cgaagaccgg atgatgctgt
     1921 ccgccgccgt cgtacgcgag ggcaggcgcc tggcgcggta ccgggtgctg aacgacgtcg
     1981 tgatcaacaa gggcgcgctc gcgcgcatga tggaactccg tgtctcggtg agcgacggcc
     2041 ctttgacgac cttgcgggct gacggcctca tcgtggcgac gccgacgggc tcgacggcct
     2101 attcgctctc cgcgggaggg cccatcatcc atccgacggt ccactgtttc gtcgttacgc
     2161 ctatatgccc gcatacgctc tcgaacaggc cgatcgccct gcccgattcg gtcgtggtcg
     2221 aggtctgcct gacctcgccg agtgaagacg tgtccatcac gctcgacgga cagatcggtt
     2281 ttcctctcgt tcagcacgac gtagtggaga tcaaaaagtc ccgctacagg atgaagctga
     2341 tcaaacatcc ggtaaagagc tactacgaaa ttctccggac gaagctcaag tgggggaatt
     2401 gaaactaata gagttccgag ttcaaagttc ggagttcgga gtacagaaga caaaaacatt
     2461 tcatggatga aatccggcaa gaattcctcg acatcgtgac ccgagtcagg gcccacctcg
     2521 aatatcagaa ggcactcggc gtgcgtcata tcgagacggc ccctgccgcg gctgtccgga
     2581 cccctcttca acaggctgac gggagagcgc ctgcggacgc ggacggacga acatcggcca
     2641 ccctgaaagg tccggcagac ggactcgcgg cagtgggcgg agaactcggc gactgcaccc
     2701 gctgcaaact gcataagggc agatcgaaca tcgtgttcgg agagggaaat cccaaggcgg
     2761 ttctcgtttt
//
LOCUS       DASSLD010000531         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_587929,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000531 DASSLD010000000
VERSION     DASSLD010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587929"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFK23_09585"
     CDS             complement(<1..273)
                     /locus_tag="VFK23_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HET7319375.1"
                     /translation="MKVILQDVTKVESEAVVVGFFEDVRPLKGLAGRLDWLLCGSLSS
                     LLISKKLRGSLGEVALLTSRGKVPAQKVFLVGLGPASGFTLPALRSA"
     gene            complement(296..727)
                     /gene="nusB"
                     /locus_tag="VFK23_09590"
     CDS             complement(296..727)
                     /gene="nusB"
                     /locus_tag="VFK23_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET7319376.1"
                     /translation="MKRRKAREHALQILFQLDLRKEKPSAASLRRFWTEFQPEEDVKA
                     FAEEIIKGTYKHLPKINDLIHQCAKNWSLDRMAVVDRNVLRMAIYEILYRIDIPTSVS
                     INEAIEIAKKYGTEESGAFVNGILDRVARLTGKLEENRIED"
     gene            complement(786..1250)
                     /gene="ribE"
                     /locus_tag="VFK23_09595"
     CDS             complement(786..1250)
                     /gene="ribE"
                     /locus_tag="VFK23_09595"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET7319377.1"
                     /translation="MPKIIQGDLSGKGLKFGIVAARFNDFITARLLDGAVDALQRHGV
                     AESDIEVVKVPGSYEIPLAARMLAQTKKYNAVICLGAVIRGATPHFEYVSAEVSKGVA
                     AVSMETGLPVIFGVLTTDTIEQAIERAGTKSGNKGWDAALSAIEMANVVKQL"
     gene            complement(1311..2516)
                     /locus_tag="VFK23_09600"
     CDS             complement(1311..2516)
                     /locus_tag="VFK23_09600"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET7319378.1"
                     /translation="MKFNTIEEAIEDIKKGKMVILVDDEDRENEGDLTMAAEMVTPEA
                     INFMARFGRGLICLSLTPERVEHLQLPMMTTDNTSSFGTAFTISIEAKKGVTTGISAA
                     DRAITIKTAIDPKKGPEDLARPGHVFPLRAKPGGVLQRAGQTEGSVDLARLAGLRPAG
                     VICEIMNDDGTMSRVPQLMEFAKTHRMKIVTIKDLIAYRVKKESLVRRVAVTKLPTSY
                     GNDFTAILYANDIDRQSHIALVKGEIKPEDEVMVRVHSECLTGDVFGSMRCDCGEQLH
                     NAMQMIDQEGKGVILYMRQEGRGIGIEGKLKAYELQDKGKDTVEANLALGYKADLRDY
                     GVGAQILRDLGVRRIKLLTNNPKKIIGLEGYGLTVTGRVPIEMHPHAKNIHYLKVKKN
                     KLGHMLEKV"
BASE COUNT          456 a          841 c          742 g          635 t
ORIGIN      
        1 cgcgctcctg agcgccggca aggtaaaccc ggaggcggga ccgaggccga ccaggaaaac
       61 cttttgcgcg gggaccttcc cccgcgaggt caggagcgcc acctcgccga gcgaaccccg
      121 cagctttttt gagatcagca ggctggataa agatccgcac aggagccagt cgagtcgtcc
      181 ggcaaggccc ttgagcggcc gcacgtcttc gaagaaaccg acgaccacgg cttcgctctc
      241 gacctttgta acgtcctgaa gaatgacttt catcgtcaat ccgggcatgc tgcgactaat
      301 cctcgatcct gttttcttcc aatttccccg tcaggcgggc gacacggtcc aggatgccgt
      361 tcacgaaggc cccggattct tccgtgccgt atttcttggc gatttctatg gcctcgttga
      421 tcgatacgct ggtgggaatg tcgatgcggt agagaatttc atagatcgcc atcctcagga
      481 cattgcgatc gacgaccgcc atgcggtcaa ggctccagtt cttggcgcac tgatgaatga
      541 ggtcgttgat cttgggcagg tgcttgtatg tccccttgat gatctcttcc gcaaaagcct
      601 tgacgtcctc ctcgggctga aactccgtcc agaagcgcct gagcgaagcg gccgaaggtt
      661 tctctttcct gaggtcgagc tgaaacagta tctggagcgc gtgctccctc gccttgcgtc
      721 tcttcatcta gaatgccgtt ctccgattgc ctggccgccg ttttccgccg cgcatcgcgt
      781 ttccatcaca actgtttcac gacgtttgcc atctcgatgg ccgacaacgc agcatcccag
      841 cctttgttgc cgctcttggt ccccgcgcgt tcgatggctt gttcgatggt atcggtggtc
      901 agcacgccga agattacggg caggcccgtc tccatggaca ctgcagccac acccttgctc
      961 acttctgcgc tcacgtactc gaagtgcggc gtcgcccccc ggatgaccgc gccgagacag
     1021 atcaccgcgt tgtacttctt cgtctgcgca agcatccgcg ccgcaagggg gatctcgtag
     1081 gacccgggga ccttcacgac ctcgatgtcg ctctcggcaa cgccgtggcg ctgcagcgca
     1141 tcgacggctc cgtcaagaag ccgggcagta atgaaatcgt tgaacctcgc ggcgacgatc
     1201 ccgaacttga ggccttttcc gctcagatct ccctggatga tttttggcat cacgtcctcc
     1261 gctctttgtt ggatttactc cgaactccga actttgtact ctgaacttgt ttagaccttc
     1321 tccagcatat gccccagctt gttcttcttg accttgaggt agtgaatgtt ttttgcgtga
     1381 gggtgcattt cgatcggcac gcgtcccgtc acggtcaacc cgtagccctc gaggccgatg
     1441 atcttcttcg gattgttcgt caggagcttg atcctgcgaa caccgaggtc tcgcaggatc
     1501 tgggcgccga cgccgtagtc ccggaggtcg gccttgtagc cgagcgcgag gttggcctcg
     1561 accgtgtcct tgcccttgtc ctggagttcg taggccttga gcttgccctc gataccgatg
     1621 ccccgccctt cctggcgcat atagaggatc acgcccttgc cctcctggtc tatcatctgc
     1681 atggcgttgt ggagctgttc cccgcagtcg caccgcatcg agccgaaaac gtcccccgtg
     1741 agacactccg aatggacccg taccatcact tcatcttcag gtttgatttc gcccttcacc
     1801 agcgcgatat ggctctggcg atcgatgtcg ttcgcgtaca ggatcgccgt aaagtcgttc
     1861 ccgtagctcg tcggcagttt cgtgaccgcc acgcgccgga cgagggactc tttcttgacg
     1921 cggtaggcaa tgaggtcctt gatggtcacg atcttcatcc ggtgcgtctt cgcgaattcc
     1981 atgagctgcg gcacgcggga catggtgccg tcgtcgttca tgatctcgca gatcacgccc
     2041 gccgggcgga gccccgcgag ccgcgcgagg tccaccgagc cctcggtctg gccggcgcgc
     2101 tgaagaacgc cgccgggctt tgcgcggagc ggaaagacgt gccccggccg ggccaggtct
     2161 tccggcccct tcttcggatc gatcgcggtc ttgatcgtga tcgcgcggtc ggccgcggaa
     2221 atgccggtgg taacgccctt cttcgcctcg atggatatgg tgaatgccgt gccgaaggat
     2281 gaggtattat cggtggtcat catgggcagt tgcaaatgtt ccacgcgctc cggtgtgagc
     2341 gagaggcaga tgaggcccct gccgaacctg gccatgaagt tgatggcctc gggggtcacc
     2401 atctcggctg ccatggtcag gtcgccttcg ttttcccggt cctcgtcgtc gacgagtatg
     2461 accatcttcc cctttttgat gtcttctatg gcttcttcaa tggtattgaa cttcataaaa
     2521 tgcctttctt tttcctcaca aattccgcat tccgcatcag cgtggcccgc ttgatcggcc
     2581 atccgaaatc agcattgaat tcatgcgaac cctgtcttct tcagcagttc caggctcacg
     2641 ccgccttcga cccttccgga caaaagccgc tcca
//
LOCUS       DASSLD010000532         6911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_956307,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000532 DASSLD010000000
VERSION     DASSLD010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956307"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1100)
                     /locus_tag="VFK23_09605"
     CDS             complement(171..1100)
                     /locus_tag="VFK23_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HET7319379.1"
                     /translation="MRILVTGGAGFLGSHLCERLLSDGHEVICLDNFFTGRKNNVQHL
                     FGNPVFELLRHDVTLPIVIEVDRIFHLACPASPIHYQYNPVKTIKTNIMGTLYMLGLA
                     KRVKGRILFASTSEVYGDPSVHPQKEDYWGNVNPIGIRSCYDEGKRASETLMMDYHRQ
                     NMVDIRIVRIFNTYGPRMLPDDGRVVSNFIIQALKGKDITVYGKGLQTRSFCYVDDLI
                     EGMVRMMNAEGLTGPVNMGNPEEYTILDLARKIIDMTGSKSKIVHMPLPSDDPTQRQP
                     DITLAGQKLGWKPSVSVDEGLRRTIEYFRKEME"
     gene            complement(1228..2562)
                     /locus_tag="VFK23_09610"
     CDS             complement(1228..2562)
                     /locus_tag="VFK23_09610"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET7319380.1"
                     /translation="MKISVIGTGYVGLVTGTCLAESGNTVLCMDIDKQKIDLLNSGVV
                     PIYEPGLEELIKRNTAHGRLGFTTDISRAVHEASVIFIAVGTPPGEDGSADLKYVLGA
                     AVDIGRHMNGYKIIVNKSTVPVGTADKVQTLVAGKTKHKFEVVSNPEFLKEGSAIEDF
                     MKPDRIVIGANSARAVRIMHNVYAPFIRKGDRSIIMDVRSAEMTKYASNAMLATKISF
                     INEMASICANLGADIDSVRRGMGYDKRIGFEFLFPGVGYGGSCFPKDVKALVQTAKDH
                     GFDAKVLKAVEAVNERQKRLLSDLILAHFTVKRSGNVARSGKPLSGRTIAIWGLSFKP
                     RTDDMREAPSVVIMNNLLRAGAKIRAHDPVATREARKIFGDKIRLFENSYETLRDADA
                     LAVVTEWNEFRTPDFDRIRKILKKPVIFDGRNIYNQEELKRMGFSYYGIGRR"
     gene            complement(2728..3864)
                     /gene="rfbB"
                     /locus_tag="VFK23_09615"
     CDS             complement(2728..3864)
                     /gene="rfbB"
                     /locus_tag="VFK23_09615"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET7319381.1"
                     /translation="MNDQTPAASFDPRSANILVTGGAGFIGSNFIRYVLEHTDFRGTL
                     VNYDKLTYAGNLMSLSDIERKFGGKGYVFERGDIKDHENVTAVMERHRISAVVHFAAE
                     SHVDRSIYGPKEFVETNVNGTFSLLEAARRLWKAGVPARFHHVSTDEVYGSLGDTGQF
                     LETTPYDPRSPYSASKASSDHLVRAYFHTYGLPVSISNCSNNYGPFHFPEKLIPLAIL
                     NAAGGKPVPVYGDGKNVRDWLYVEDHCEAVWRILAGGRAGETYNVGGECEKQNIEVVT
                     AICDALEKLYPAKDNAALRTPHFAIRNYKDLITFVPDRPGHDRRYAINCDKIKRELGW
                     KQRHRFEVGLRHTVDWYLKNTVWVESVRSGEYAKWIEKNYAERK"
     gene            complement(3857..4717)
                     /gene="rfbD"
                     /locus_tag="VFK23_09620"
     CDS             complement(3857..4717)
                     /gene="rfbD"
                     /locus_tag="VFK23_09620"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HET7319382.1"
                     /translation="MKYLVTGGKGQLARSFVRRLEKLCRDCIVPDESRLDITNEAAVS
                     ECVDAAKPSVIINCAAYNLVDKAEDAPIAAYAVNAAGPKYLAQAAARHKAVLVHFGSD
                     YVFDGLKEQGPYTEDDATNPLNEYGKSKLAGERHVLEELDQCLILRLSWVFGPGKQNF
                     IYKLTQWAVSNEYLKIACDEFSVPTSCETVVDVTLKALDRGLTGRYHLTNSGFCSRYE
                     WARMILQSLGIDKFVRPVSMDSFHLAAKRPQFSAMSNDRISRLLGLSIPSWEEGVQSY
                     LKEGGLTHNE"
     gene            complement(4763..5338)
                     /gene="rfbC"
                     /locus_tag="VFK23_09625"
     CDS             complement(4763..5338)
                     /gene="rfbC"
                     /locus_tag="VFK23_09625"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HET7319383.1"
                     /translation="MPFEFKNFEIPGPLLIKPRVFDDDRGFFLELYKHSDFAGAGIRK
                     HLVQDNYAKSTKGVLRGLHYQMGPKAQGKLVVCMQGGIYDVAVDIRKGSPHFGKWVGI
                     ELTENNRLMLFVPPGFAHGYQVLSEASEVMYKCTDEYSPTHDRGIIWNDPAIGIAWPL
                     PDPVLSPKDKLHPLLRDADNNFVWSEPTALA"
     gene            complement(5328..6221)
                     /gene="rfbA"
                     /locus_tag="VFK23_09630"
     CDS             complement(5328..6221)
                     /gene="rfbA"
                     /locus_tag="VFK23_09630"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET7319384.1"
                     /translation="MKGIILAGGSGTRLYPITRGVCKQLLPIYDKPMIYYPLSVLMLA
                     GIRDILIISTPVDLPRFKDIFGDGTDLGLRFSYREQLHPNGLAEAFLIGEDFVGNDTA
                     CLVLGDNIFFGHGLTELLRKAVRDVTTAGGATVFGYYVNDPERYGIVEFDKDGTVLSV
                     EEKPKHPKSKYAVTGLYFYDNEVVRIAKRIKPSGRGELEITDVNKEYLAKKQLRVELL
                     GRGYAWLDTGTHESLLEASEFIATIERRQGLKMACIEEIAYKQGYIDKQKLLKAAEIH
                     NKNAYGAYLKMVAEQEVPSAL"
     gene            complement(6295..>6911)
                     /locus_tag="VFK23_09635"
     CDS             complement(6295..>6911)
                     /locus_tag="VFK23_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET7319385.1"
                     /translation="FDRETRDGIGKEIHNRIFDHVKRRVAEGLDGIVVSDYTKGVVTA
                     DMVRDIVRISRKNDIIVSVDPKVNHFGMYRGVTILTPNVNEASIGSRIEILDEDSLLR
                     AGALLLKKLNCDAVLITRGEQGMSLFERGGRVTHIPTVAREVFDVTGAGDTVIGALTL
                     AMASGAGMVDAARISNYAAGIVVGIVGTASVKPEELKQKIVHSS"
BASE COUNT         1275 a         2009 c         1995 g         1632 t
ORIGIN      
        1 cgagcgcctt gccggggaac cgcgtggagc cgtatcgggc ggggatgagg gctatgatgg
       61 ttgttttccg catagtgcat tgagcaaagc gcatagcgaa aaacgcaaag tgcaaagtgc
      121 agaacgcagt atccttacgc catgccccat gccccatgcg ccgtgacgct ctactccatt
      181 tccttcctga aatattctat cgtcctccgc aatccttcgt ccacggacac ggagggcttc
      241 catccgagct tctgcccggc gagcgtgatg tcgggctggc gctgggttgg atcgtcggag
      301 ggaagcggca tgtgcacgat ctttgacttc gatccggtca tgtcgatgat cttgcgtgcc
      361 aggtcgagga tagtgtattc ctcgggattc cccatgttca cggggcccgt aaggccttcc
      421 gcgttcatca tgcgcaccat gccttcgatc agatcgtcga cgtagcagaa cgacctcgtc
      481 tgcagaccct ttccgtagac cgttatgtct ttccccttga gcgcctgaat gatgaagttg
      541 ctcacgaccc ggccgtcgtc gggtagcatg cgggggccgt aagtgttgaa gatgcgcacg
      601 atcctgatgt caaccatgtt ctggcggtga taatccatca tcagggtctc cgaagcccgt
      661 ttcccctcgt cgtagcagct ccggattccg atggggttca cgttgcccca gtagtcctcc
      721 ttctgcgggt ggaccgaggg gtcgccgtag acctccgagg ttgaggcgaa gaggatcctt
      781 cccttgaccc tcttggcgag gccgagcatg tagagcgtgc ccatgatgtt cgtcttgatc
      841 gtcttgaccg ggttgtactg gtagtggatg ggcgacgcgg gacaggccag atggaagatg
      901 cggtccacct cgatgacgat cggaagcgtc acatcatgac ggagaagttc gaaaacggga
      961 tttccgaaga ggtgctgcac gttattcttc ctgccggtaa agaaattgtc caggcagatg
     1021 acctcgtggc cgtcagaaag cagccgttca cacaggtgtg agcccagaaa tcccgcgccg
     1081 ccggtaacga gtattcgcat gatgcattct cccttggaaa tgattgatgg acgacggacg
     1141 accggtcgcc ttgctcacca ggatgacgga cgaaaaagct tttgcagctc tcgtccctcg
     1201 tccgtcgtct ctcgtccttt cgctgcgcta tcttctcccg atgccgtaat agctgaaacc
     1261 cattcttttc agctcttcct ggttgtaaat atttctgccg tcaaagatga ccggtttttt
     1321 caggatcttc cttatcctgt cgaaatccgg cgtgcggaat tcattccatt cggtcaccac
     1381 cgcgagcgcg tcggcgtccc ggagtgtttc gtagctgttt tcgaagaggc gaatcttgtc
     1441 cccgaagatt tttctcgcct ctcgcgttgc caccggatcg tgcgccctga tctttgcgcc
     1501 ggcgcgcaga aggttgttca tgatcaccac cgaaggcgcc tcgcgcatgt catcggtgcg
     1561 cggcttgaag gaaagccccc agattgcgat ggtgcggccc gagagcggct ttccggatcg
     1621 tgcaacgttc cccgaacgct tcactgtgaa atgggccaga atgagatcgg agaggagacg
     1681 tttttgacgt tcattcaccg cttcgacggc cttcaggacc ttggcgtcga atccgtggtc
     1741 ttttgccgtc tgcaccagcg ccttgacgtc cttcggaaag caggaccctc cgtagccgac
     1801 gcccgggaac aggaattcga acccgatccg cttgtcatac cccatgcccc ggcggacgct
     1861 gtcgatatcg gccccgaggt tcgcgcagat gctggccatt tcgttgataa aggatatttt
     1921 tgtcgccagc atggcgttgg aggcgtattt cgtcatctcc gcgctcctca cgtccatgat
     1981 gatcgagcgg tcccctttgc ggatgaacgg cgcgtagacg ttgtgcatga tcctcaccgc
     2041 cctggcgctg ttcgcgccga ttacgatcct gtccggcttc atgaagtctt caatcgccga
     2101 cccttccttc agaaattcgg gattggacac cacctcgaat ttgtgcttcg tcttgccggc
     2161 gacgagggtc tggaccttgt cggccgtacc cacggggacg gtactcttgt tgacgatgat
     2221 cttatagccg ttcatgtgac gcccgatgtc cacggccgca ccaagcacgt atttgaggtc
     2281 agccgagccg tcctcgcccg gaggggtccc cacggcaatg aatatcacgc tcgcctcgtg
     2341 caccgccctt gatatgtcgg tggtaaagcc gagcctgccg tgggcggtgt tccgcttgat
     2401 gagctcttca agacccggct cgtaaatggg gacgactccg ctgttcaaga ggtcgatctt
     2461 ctgtttgtcg atgtccatgc agaggacggt gttgccgctc tcggcaagac aggtaccggt
     2521 caccaggccc acgtagccgg tgccgataac ggatattttc atgagggtcc tttctccttt
     2581 tgtcatcccc gaatgtttct atcggggatc cggtttggtt tgtctgtgcc gggcgttgcc
     2641 ttcgataaaa accagattcc cgattaaagc ttcgggaatg acaggttttg ttgcgttata
     2701 tcgctgctat caaccatgag ctatcagcta ttttctctca gcataattct tctcgatcca
     2761 cttcgcatat tcgccgctcc tgacactctc cacccatacc gtgttcttca ggtaccagtc
     2821 gaccgtatgc cgaagcccaa cctcgaaacg gtggcgctgc ttccagccga gttcccgttt
     2881 gatcttatcg caattgatgg cgtatcggcg gtcgtgtccg ggccggtcgg gtacgaacgt
     2941 gatgagatct ttgtaattgc ggattgcgaa gtgcggagtg cggagtgccg cattgtcctt
     3001 cgcgggataa agtttttcca gcgcgtcgca gattgcggtg accacctcga tgttctgctt
     3061 ctcgcactcg ccgcccacat tatatgtctc acccgccctc cccccggcga gtatccgcca
     3121 gaccgcctcg cagtggtctt cgacgtagag ccagtcgcga acgttcttcc cgtcgccata
     3181 aacagggacc ggctttcccc ccgcggcgtt caggatcgcg agcggtatga gcttttcggg
     3241 gaaatgaaag ggcccgtaat tgttcgagca gttcgagata gagacgggca ggccgtaagt
     3301 atggaagtag gccctcacca gatggtccga cgaggccttg gacgccgaat aggggctccg
     3361 cgggtcgtac ggcgtcgttt ccaaaaactg ccccgtgtcg ccgagggagc cgtaaacttc
     3421 gtcggtgctg acgtgatgga accgggccgg gacaccggcc ttccagaggc ggcgtgcggc
     3481 ttccaggagg ctgaaagtgc cgttgacgtt cgtctcgacg aactccttgg gcccgtagat
     3541 cgaccggtcg acatggcttt ccgcggcgaa gtggaccacg gcgctgatgc ggtgcctctc
     3601 catgaccgcc gtgacgttct catggtcctt gatgtccccg cgttcgaaga catagccctt
     3661 tccgccgaat ttccgctcga tatcggacaa gctcatgagg ttgccggcat aggtcagctt
     3721 gtcgtagttg acgagcgtgc ccctgaagtc agtgtgttca aggacgtacc gtatgaaatt
     3781 ggacccgatg aacccggccc cgccggtcac gaggatgttc gcggaccggg gatcgaaaga
     3841 cgccgctggt gtttggtcat tcattatgag tcaacccgcc ttccttcagg tatgattgaa
     3901 ctccttcttc ccaggacggt atggacaggc cgagcagccg cgagatcctg tcgttgctca
     3961 ttgccgaaaa ttgcggacgt ttcgccgcga ggtgaaagga atccatggaa acgggcctga
     4021 cgaatttatc gataccgagg ctttggagta tcattctggc ccattcatac cgggagcaaa
     4081 aaccgctgtt ggtgagatgg taccgtccgg tcagcccccg gtcgagcgcc ttgagtgtaa
     4141 catccaccac ggtctcgcag gaggtgggga ccgaaaactc gtcgcaggcg atcttaaggt
     4201 actcgttgct gacggcccac tgggtcagtt tatagatgaa gttctgcttt cccggcccga
     4261 acacccagct cagcctcagg atgagacatt ggtcgagttc ctccaggaca tgtcgctccc
     4321 cggcgagttt gctcttcccg tattcgttca gcgggttcgt cgcgtcgtcc tcggtgtagg
     4381 gcccctgctc cttcagcccg tcgaagacgt agtccgaccc gaagtgcacg agaaccgcct
     4441 tgtgccttgc agccgcctgc gcgagatatt tgggacccgc cgcgttgacc gcgtacgccg
     4501 ctatgggcgc gtcttccgcc ttatctacga ggttgtaagc cgcgcagttg ataataaccg
     4561 acggtttcgc cgcgtcgacg cattcggaga ctgccgcctc gttcgtgatg tcgagccgcg
     4621 actcgtcggg gacgatgcaa tctcggcata acttttccag ccgcctgacg aacgatctgg
     4681 cgagctgtcc tttcccgcct gttacgaggt atttcatgat tttttttgga ccaaggcaat
     4741 aaggcctcgg cgttcccgta atttatgcca acgcagttgg ctcgctccac acaaaattat
     4801 tgtccgcatc ccgaaggagc ggatgcagtt tgtccttggg cgacaggacc gggtccggga
     4861 gcggccaggc gatcccgatc gcggggtcgt tccagatgat accccggtca tgggtcggag
     4921 aatattcatc ggtgcatttg tacatgactt cagaggcctc ggagagaacc tggtacccat
     4981 gggcgaaacc cggcggcacg aagagcatga gcctgttatt ttcggtcagt tcaatgccca
     5041 cccacttccc gaagtgcggg gaacccttgc ggatatccac ggcgacgtca taaataccgc
     5101 cctgcatgca cacgacgagc ttgccctgtg ccttggggcc catctggtag tggagccccc
     5161 ggagcacgcc tttggtggat tttgcatagt tgtcctggac taagtgtttg cgtattccag
     5221 ctcctgcaaa gtccgaatgt ttgtacagct cgaggaaaaa tccgcggtcg tcgtcgaaga
     5281 cccggggctt gatcaatagg ggccccggaa tctcaaagtt tttgaactca aagggcactc
     5341 ggcacctcct gctccgcgac cattttcaga tacgctccgt aggcgttctt gttgtggatc
     5401 tccgcggctt tcaggagttt ctgcttgtcg atgtagccct gtttgtatgc aatctcctcg
     5461 atgcaggcca ttttgaggcc ctgccgccgt tctatggttg cgatgaactc gctggcttcg
     5521 agcagactct cgtgggtccc cgtgtccagc caggcgtacc ctcttcccag cagctccacg
     5581 cggagttgct tcttcgcgag gtactccttg ttcacgtccg tgatttccag ctctccgcgc
     5641 cccgacggct tgatcctttt cgcgattcgg accacttcgt tgtcgtagaa atagaggccg
     5701 gttacagcgt atttagactt cgggtgcttc ggtttttctt ccacggacag cacggtgccg
     5761 tccttgtcga attccacgat gccgtagcgt tcgggatcat tcacgtaata accgaagact
     5821 gttgcgccac cggcggtcgt cacatcgcgg actgcctttc tgagcagttc ggtgaggccg
     5881 tggccgaaaa aaatgttgtc tcccagtaca aggcaggccg tatcgttgcc gacgaagtcc
     5941 tcgccgatga ggaacgcctc ggcaaggccg ttcggatgca gctgttcccg gtaggaaaaa
     6001 cgaagtccga ggtcggtccc gtcgccgaag atgtccttga atcgtggaag gtcgacgggc
     6061 gtcgagatga tgaggatgtc ccggatgccg gcgagcatga ggactgacag cgggtagtag
     6121 atcatgggct tgtcatagat cggcagaagc tgcttgcaga ctccgcgtgt aatgggatag
     6181 agacgcgttc cgctgccgcc ggccaagata atgcctttca tgaggttctc cttgggcagt
     6241 tcagagttca aagttcaggg ttcagagtaa cagcaaagca ggggttcgtc ttgactatga
     6301 actgtgaact attttttgct ttaattcctc cggctttacg gaggccgttc ctacgatacc
     6361 cacgacgatc cccgccgcat agttcgagat ccgcgcggcg tccaccatgc cggcgccgga
     6421 agccatggcg agggtaaggg cgccgatgac tgtgtctcct gcgccggtca cgtcgaagac
     6481 ctcccgcgcc acggtcggga tgtgggtgac ccttcccccg cgctcgaaga ggctcatccc
     6541 ctgctccccg cgggtgatca ggacggcgtc gcagttcagc ttcttgagga gcagcgcccc
     6601 ggcgcggagc agggagtctt cgtccaagat ctcgatcctg gacccgatgg acgcctcatt
     6661 gacgttcggc gtgaggatgg tgaccccgcg gtacatgccg aagtggttca ccttggggtc
     6721 cacggacacg atgatgtcgt tcttccggga gatgcgcacg atgtcgcgca ccatgtcggc
     6781 ggtgaccacg cccttggtgt agtccgagac cacgatgccg tccagcccct ccgcaacgcg
     6841 gcgtttgaca tggtcgaata tgcggttgtg gatctctttt cctatcccat ccctggtctc
     6901 gcggtcgaac c
//
LOCUS       DASSLD010000533         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1544762, whole genome shotgun sequence.
ACCESSION   DASSLD010000533 DASSLD010000000
VERSION     DASSLD010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544762"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFK23_09640"
     CDS             complement(<1..216)
                     /locus_tag="VFK23_09640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7319386.1"
                     /translation="MSLAVNSVALPTQADVAIVGAGAMGLFTALSLTTAGRKVIVIDK
                     ERPWREASGVNAGSLGIQNKRQALVPFA"
     gene            complement(213..1007)
                     /locus_tag="VFK23_09645"
     CDS             complement(213..1007)
                     /locus_tag="VFK23_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET7319387.1"
                     /translation="MGEPMIDVSNVHKCFGPNKVLKGVSLKVERGQVVALIGPSGSGK
                     STFLRCINALEKPDNGRIRVGDHVIDCSEGRTILHSDRNLAAFRSNVGMVFQHFNLFP
                     HMTALGNVMEGPVTVKRISKTEAGKLGRDLLARVGLADKENEFPSRLSGGQRQRVAIA
                     RALAMNPQVILLDEVTSALDPELVGEVLEVIRRLAKDGMTMIIVTHEISFAHEVANIV
                     GFMSDGVMAELGPPSLVLEKPVNERTKAFLARFHSFHNAYEERQSE"
     gene            complement(1020..1685)
                     /locus_tag="VFK23_09650"
     CDS             complement(1020..1685)
                     /locus_tag="VFK23_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET7319388.1"
                     /translation="MDYHWSFGFLWEFRSLILKGLLYSILYTVGTILLGLVIGLVTGL
                     ARLSRNRFVTLPIAAYVEVFRCTPLLVQLVWMYYALPVVVGIEIPAYLAAILALSLYV
                     GSFYSEIFRGGVKSIESGQWDAARALGMTWVQVMRRVVLPQAVKRMTPPFINQSVIQL
                     KNTSLVSTLAVADLMYQGTIITAATYRPLEVYTLIAVLYFLILFPATWLAQVYEGRLA
                     KSD"
     gene            complement(1786..2631)
                     /locus_tag="VFK23_09655"
     CDS             complement(1786..2631)
                     /locus_tag="VFK23_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET7319389.1"
                     /translation="MLRGKRRMVIAFISAVCLGLTLFTTISFAADKGESTMERIKRTK
                     VLRVAGLVGEEPYFHKDIKTGEWSGFCIVMARDLAKDLGAKLEVVESTWGNSVLDLQA
                     NKIDISFALNPTPQRRQVIDFSDPLFNNSFIIVTRKDFKVGTWEEMNNPKVRVAVDIG
                     SSHEAITRKNAPNATITGYKTRDEAILAVQANKADCFVATVFLGLTALKKNPQLGNFI
                     IPKPVATVPVSAGVRKEEDKQFRDFVGKWAAKNNKSGKIRQWIIEALGTVGISPSDVP
                     AEVQF"
BASE COUNT          612 a          833 c          788 g          590 t
ORIGIN      
        1 cgcgaatggg acaagcgctt gccgcttgtt ctggatgccc aaggaacctg cattgacgcc
       61 cgaagcctcg cgccacggcc gctccttgtc gatcacaatg accttgcggc ctgccgtggt
      121 aaggctaagc gccgtgaata accccattgc gcccgcgcct acaatcgcaa cgtccgcctg
      181 ggtgggaagt gccacggagt tgacagcaag actcattcgg attggcgctc ctcgtaggca
      241 ttatggaagg agtggaagcg ggccaagaag gcctttgtcc gctcgtttac cggtttctca
      301 agaaccaggg aagggggacc aagttccgcc atgaccccat cgctcatgaa ccccacaatg
      361 ttggccacct cgtgggcaaa actgatctcg tgggtgacga ttatcattgt catcccatcc
      421 ttggcgaggc ggcggatcac ttcgagcacc tcgccgacca gttcggggtc gagagcggag
      481 gtcacctcgt cgagaagaat aacctggggg ttcatcgcca gcgcccgggc gatggcaacc
      541 cgctggcgct gtccacccga gagacgggag ggaaactcgt tctccttatc ggccaaccct
      601 acccgggcga ggaggtcccg gcccagtttc ccggcctccg tttttgaaat ccgcttgacg
      661 gtaaccggcc cctccattac gtttccaagc gccgtcatgt ggggaaacag gttgaagtgc
      721 tggaacacca ttccaacgtt gctgcggaat gcggcaagat ttcgatcaga atgaaggatt
      781 gtccgccctt ccgaacagtc gataacgtgg tccccaaccc gtatccggcc gttgtcgggc
      841 ttctcgagtg cgttgatgca acgcagaaaa gtcgatttcc ccgagccgct cggcccgatc
      901 aaggccacca cctgcccccg ctcgaccttt aaggaaaccc ctttcagaac cttattaggg
      961 ccgaaacact tgtgcacgtt cgaaacgtcg atcatcggtt cacccatgga cacccctttt
     1021 cagtcactct ttgccaatct gccttcgtat acctgggcaa gccaagtcgc ggggaataag
     1081 atcaggaaat aaagcaccgc gatcagggtg tagacctcaa ggggccgata cgttgccgca
     1141 gtgatgatcg tgccctggta catcaggtca gcgacggcaa gcgtggaaac gagtgatgtg
     1201 ttcttgagct ggatgaccga ctggttgatg aacggcgggg tcatgcgctt gaccgcctgt
     1261 ggcaggacaa cccggcgcat cacctgtacc caggtcatcc ccaatgcccg tgcggcatcc
     1321 cactgcccac tctcgatcga cttgacgccc ccgcggaaaa tctccgagta aaacgaaccg
     1381 acatagagcg acaaggcgag gatggctgcc aagtatgcag ggatctcgat gcccacaaca
     1441 accgggagcg cgtagtacat ccacacaagc tggacaagta gcggcgtgca gcggaatacc
     1501 tccacgtacg ccgcgatggg taacgtcacg aaacggtttc gtgacaggcg cgccaggcct
     1561 gtgaccagcc caatcacgag gcccaacagg atcgtgccta ccgtgtaaag gatagaatag
     1621 agcagcccct tcaagatcag gctgcggaac tcccagagga aaccgaagct ccaatgatag
     1681 tccaaggcat ttccttcgtt gtgaacgtgg gggttagtcc cggaaagccg gcgcggcgcg
     1741 cccccaatac ggagcgcgcc gcgccggtaa cctgaacatt cttaactaga actgaacctc
     1801 ggcgggaaca tcggatgggg agatccccac ggttcccaag gcctcgatga tccattgccg
     1861 aatctttccg ctcttattgt tctttgccgc ccatttccca acgaagtcgc ggaactgttt
     1921 gtcttcttcc ttgcgtaccc ccgccgagac cggcacagtg gccacgggct tgggaatgat
     1981 gaagttgccg agctggggat tctttttaag ggcggtgagg cccaggaata cggtcgccac
     2041 gaagcagtct gctttatttg cctgaacggc gaggatcgcc tcatccctgg tcttgtaccc
     2101 ggtgatcgtc gcattgggtg cattctttcg agtgatcgct tcgtgggagg acccgatgtc
     2161 gacggcgacg cgtaccttgg ggttattcat ctcctcccaa gtacctactt tgaaatcctt
     2221 gcgagtgacg atgatgaagc tgttgttgaa caagggatcg gagaagtcga tgacctgtct
     2281 gcgctgcggc gtcgggttca gagcgaagga gatgtcgatc ttgttggcct ggaggtcgag
     2341 cacggaattg ccccaagtcg attcgactac ctcaagtttg gcgcccagat cctttgccaa
     2401 atcacgcgcc atgacgatac agaaaccact ccactccccc gttttgatgt ccttgtggaa
     2461 gtagggctcc tcgccgacaa gccccgccac gcgaaggacc ttcgtccttt tgatccgctc
     2521 catcgttgat tcgcccttgt ccgcagcgaa tgagatggtg gtgaacaagg tgaggccgag
     2581 gcataccgct gagatgaacg cgatcaccat acgccgtttc cctctgagca tctggatctc
     2641 ctctctttgg tttgtctata cagactaaat tagggaaaat cctaactctg acaataagct
     2701 ttttaggacc gcccggtctg agtccggaat cgaaagcagg gggggccgcg gccatccccc
     2761 tggacggccc aagaggcgca tcgcctcttt tattactacg taaggattgt atccccactg
     2821 ata
//
LOCUS       DASSLD010000534         2341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_173439,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000534 DASSLD010000000
VERSION     DASSLD010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173439"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFK23_09660"
     CDS             <1..373
                     /locus_tag="VFK23_09660"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET7319390.1"
                     /translation="RDCSLGVISYENLEWTEHSVKIVDLSVTGIGIEADRLLASGIVW
                     FREHVYGQRCGFLVWSKKIGDRYRSGIQFVSLARGDEEYLRRQVSLIRPCESFQDPDQ
                     ILAKMMEDINRDRGRSANPPA"
     gene            424..1212
                     /gene="rsmA"
                     /locus_tag="VFK23_09665"
     CDS             424..1212
                     /gene="rsmA"
                     /locus_tag="VFK23_09665"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET7319391.1"
                     /translation="MDTIRAKKSLGQNFLKDPHYLRKIADAARLGPGDQVIEIGPGLG
                     HLTRVLAASAGKVLAIELDERLITPLRREFGASPNVEVVQADALGYPYASLPGKWKVV
                     ANLPYYISTAVLQELIARREKFVSFTLMLQKEVAERIASPPGGKEYGYLSVLVQLYAE
                     PRIEFTVPPGAFTPRPDVESAVVTLVLRGAPAVSVRNEDFLLRVVKAAFSQRRKTLRN
                     ALRQLDLAKEKLEAAAGMTGIDLGRRAETLSVEEFGRLADFLLE"
     gene            1458..2039
                     /locus_tag="VFK23_09670"
     CDS             1458..2039
                     /locus_tag="VFK23_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HET7319392.1"
                     /translation="MKETIVQAFEESARIKVQFARDNADKIVEVVQLIAQAFREGRKV
                     ILFGNGGSAMDASHIAAEFMNRFLIERPPLPAVSLNSDTALLTCISNDYDFSQVFSKQ
                     LAALGHEGDVVIGISTSGNSPNVIKALEVARKNGMKTVALTGGNGGRLAGLADYAFIV
                     PTKATPRIQEAHITLGHTICQLVDEELFGKHGK"
BASE COUNT          509 a          723 c          655 g          454 t
ORIGIN      
        1 ccgcgactgc agcctcggcg tcatttcgta cgagaacctc gagtggacgg agcacagcgt
       61 taaaatcgtc gatctcagcg tgaccggcat cgggatcgag gccgaccggc tgctcgcttc
      121 cggtatcgtc tggttcaggg aacatgtcta cggccaacgg tgcgggtttc tcgtatggag
      181 caaaaaaatc ggcgaccgct accggagcgg catccagttc gtttcccttg cccgcgggga
      241 cgaagagtac ctgcgccggc aggtcagcct tatccggccc tgtgaatctt ttcaggaccc
      301 ggaccagatc ctcgcaaaaa tgatggaaga cattaacaga gaccgggggc gttccgccaa
      361 tcccccagcc tgatagtttc tcccccgctg tctccgcgct atcctctcat cgacgtcgga
      421 cccatggaca cgattcgagc aaaaaaatct ctcggccaga atttcctcaa ggacccgcac
      481 tatctccgca agatcgcgga cgcggcccgg ctggggcccg gcgaccaggt catcgagatc
      541 ggcccggggc tcggtcacct cacgcgtgtg ctcgcggcct ccgcgggaaa ggtgctcgcc
      601 atcgagctgg acgagaggct gatcaccccc ctgcgccggg agttcggcgc gagcccgaac
      661 gtcgaggtcg tccaggcgga cgccctcggc tatccctatg cgtcccttcc cgggaagtgg
      721 aaggtcgtgg ccaacctgcc ctactacatt tccaccgccg tcctccagga gctcatcgcc
      781 cgtcgggaaa agtttgtgag tttcacgctc atgctccaga aggaagtcgc ggagcgcatc
      841 gcgtccccgc ccgggggaaa ggaatacggc tatctgtcgg tgcttgtcca attgtacgcg
      901 gaaccgcgga ttgaattcac ggtcccgccc ggcgcattca cgccgagacc cgacgtggag
      961 tccgcggtcg ttacgcttgt cctccgcgga gcgcccgccg tctccgtgcg caacgaggat
     1021 ttcctcctgc gcgttgtgaa ggctgccttt tcccagcgca gaaaaacgct cagaaacgcc
     1081 ttgaggcaac tggacctcgc gaaggagaag ctggaggctg cagcgggaat gaccggcatc
     1141 gatctcggcc gaagggcaga gaccctctcc gtggaggaat tcggcaggct tgcggatttt
     1201 ttgctggagt gatgcggaaa tgcgaagtgc ttgttgactc gacaacgatc acattgtggg
     1261 gtactgaaaa tcacatccac ccagcttgtc atacctgcga aagcaggtat ccattggtcc
     1321 tttattcaag aaacttcaac atggattcct gctttcgcgg gaatgacaga ataggctaca
     1381 gccttttcac cttgactttt ccccgacttg ccatataata aacgctctac cgttcagtcc
     1441 ccctgcagga gcaccgcatg aaggaaacaa tcgtacaggc attcgaggaa agcgcccgga
     1501 ttaaagtcca gttcgcgcgt gataacgcgg acaagatcgt cgaagtcgtt cagctcatcg
     1561 cacaagcctt ccgtgaaggc aggaaagtca tcctcttcgg caacggcggc agcgccatgg
     1621 acgcatcgca catcgcggcc gagttcatga accgctttct catcgaacgc ccgccccttc
     1681 ccgccgtctc cctgaactcg gacacggccc ttcttacctg catcagcaat gactacgatt
     1741 tttcccaggt cttttccaag caacttgctg ccctgggaca cgagggcgat gtggtaatcg
     1801 gcatcagcac cagcggcaac tcgccgaatg tcatcaaggc cctggaggtg gcgagaaaaa
     1861 acgggatgaa gaccgtcgcc ctgacaggcg ggaacggcgg aaggctggcc ggtctcgcgg
     1921 attatgcctt catcgttccg accaaggcga ccccgagaat ccaggaggcc cacatcaccc
     1981 tcggccacac catctgccag ctcgtggacg aggaactttt cgggaaacac gggaaatagt
     2041 cggcgactat tcagggctca atcactagtg cctgaatgcc gcaaccaaat agaaacccat
     2101 ccccacctga cctcccctcg cctagaggcg cctacttttg gtgaagggga ggaattttta
     2161 aacctctccc tcttcaaggg ggagattgag agggggatgg gccatagagc gtcatgaaaa
     2221 aaatcgatcc ctcgaaaatc aagacctact cgctcagggg ccgcaacagc aaggtgcgga
     2281 tcggcgactc cagccggccg cataagaagg gctcaagctt ccgggatttt ttcgactccc
     2341 t
//
LOCUS       DASSLD010000535         2563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_434239,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000535 DASSLD010000000
VERSION     DASSLD010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434239"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..212
                     /locus_tag="VFK23_09675"
     CDS             108..212
                     /locus_tag="VFK23_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319393.1"
                     /translation="MRTIQPEGKKPMTAAEFLRGHRGVVGKKFEGGAK"
     gene            310..1089
                     /locus_tag="VFK23_09680"
     CDS             310..1089
                     /locus_tag="VFK23_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HET7319394.1"
                     /translation="MSEPKKRIFIGLIVFTVLLITAITVALYLIPYVGLKNIHPWAPR
                     IVCLTMGAALSILILGVGLLILTLVRGREIFFASRLRGIVIEILFPIMIVVGKILGIS
                     KEKIQQSFVEVNNLLVRAKMRDAKPGRVLLLMPHCLQFNDCAIKITTDANKCQACGKC
                     KIKDLVMLAHKYHVHLSVATGGTLARRRVAEAKPHAIIAVACERDLTSGIQDAYPLPV
                     IGILNDRPFGPCFNTSVDISKVAEALEFIANGRAQTGGGNG"
     gene            1082..2404
                     /gene="rsmB"
                     /locus_tag="VFK23_09685"
     CDS             1082..2404
                     /gene="rsmB"
                     /locus_tag="VFK23_09685"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:TIGR00563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET7319395.1"
                     /translation="MDRPREKALEILRKVEDDVFADSLLEEARQSFDERDSAFVRELV
                     YGVLRNRLRLDWVLNLFSAQPIEKAGVSTRNVLRLGAYQLLFLDRVPPSAAVNTSVEL
                     AKAHGGKQGYVNGLLRNLDRKRDNIAYPDTGDPVERLSVLYSHPDWLVGRWTGRFGIE
                     TAETLLRANNAPAPIVLRANRLRTTREELKSDLERAGVHAIETAYSPAGIRIVSPPPI
                     RTLPGYEAGRFMVQDEAAQLVGLMLSPQPGERVLDACAAPGGKATHLAELMEDKGEVV
                     ALELDAARIARITENSRRLGMTIIVPKQGNAANYAEEGFDRVLIDAPCSGLGVLRRHP
                     DGRWSKSEGAVRERAALQRRILENCARLLRSGGVLVYATCSTEPEENEDVIEAFLAGP
                     GSGFVVADPRPYLPGRASELVDGNNFLHTYPKATEMDGFFGVRMVKKK"
BASE COUNT          521 a          747 c          803 g          492 t
ORIGIN      
        1 tcaagatcat cgggtctgta gtcgcggccg gcagcgggga accggggaaa tagtatcaag
       61 acgacgataa tacgctgttc gtcggcgccg gaagcggcag gctccgcatc cggaccatcc
      121 agcccgaggg gaaaaaaccc atgaccgccg ccgagttcct ccggggacac cggggagtgg
      181 tggggaagaa gttcgaggga ggagcaaaat agaatacagg agacaggagt caggagacag
      241 aagaaacgca ttttgacatg tgttctctgc tgactcctgt attctgtctc ctgtctcctg
      301 catgaaacca tgtccgagcc caaaaaaaga atcttcatcg gcctcatcgt tttcacggtc
      361 ctgctcatta ccgcgatcac cgtggcgctc tacctgatcc cctatgtcgg gctcaagaac
      421 attcatccct gggcgccgcg gatcgtgtgc ctcacgatgg gcgcggcctt gagcatcctg
      481 atcctcggtg tggggctctt gatcctgacc ctggtccgcg gcagggagat ttttttcgcc
      541 agccggctgc ggggcatcgt gatcgagatc ctctttccca tcatgatcgt cgtgggcaag
      601 attttgggca tttccaagga aaagatccag cagtccttcg tcgaggtgaa caaccttctg
      661 gttcgggcga agatgaggga cgccaagccc ggccgcgtcc tgctcctcat gccccactgc
      721 ctccagttca acgactgcgc catcaagatc accaccgatg cgaacaagtg ccaggcctgc
      781 ggcaaatgca agatcaagga cctggtgatg ctcgcccaca agtatcatgt ccacctttcg
      841 gtggccaccg gcggcacgct agcgcgaagg agggtggcgg aggcaaagcc ccatgccatc
      901 attgccgttg cgtgtgaacg ggacctgacc tccggcatcc aggacgccta tccgctgccc
      961 gtcatcggga tcctgaacga ccgtcccttc ggaccgtgct tcaatacgag cgtcgatatc
     1021 agcaaggtcg ccgaggccct tgaattcatc gcgaacggcc gcgctcaaac cggcggaggc
     1081 aatggatagg ccgcgggaaa aggccctcga aatcctccgg aaagtcgagg acgacgtctt
     1141 tgcggattcg ctcctcgaag aggcgaggca gtccttcgat gagcgtgaca gtgcctttgt
     1201 gcgggaactg gtgtacgggg tgctcagaaa ccggcttcgc ctcgattggg ttctgaatct
     1261 tttttctgcc caacctattg aaaaagcagg cgtctcgaca agaaacgtcc tgcggctcgg
     1321 ggcctaccag ctgctttttc tggacagggt ccctcccagc gccgcggtca acacgtccgt
     1381 cgagcttgcc aaggcgcacg gcgggaagca gggatacgtg aacggcctgc tccggaacct
     1441 tgaccggaag cgcgacaaca tcgcctatcc ggatacggga gatccggtag agcggctctc
     1501 tgtcctgtat tcccaccccg attggctggt cgggcgctgg accgggcgct tcggaatcga
     1561 gacggcggaa acgctcctgc gcgcaaacaa cgctcccgct ccgatcgtgc tccgggcgaa
     1621 caggctcagg acgacgaggg aagagctcaa gtccgacctt gaacgcgcgg gtgtgcacgc
     1681 aatcgagacc gcttactccc ctgcggggat cagaattgtt tcgccgccgc ctatccgcac
     1741 ccttccgggc tatgaggcgg ggcggttcat ggtgcaggac gaggctgcgc agcttgtcgg
     1801 cctgatgctt tccccccagc cgggagagcg tgtgctggac gcctgcgcgg ctcccggcgg
     1861 taaggcgacg caccttgccg agctgatgga ggacaagggc gaggtcgtcg cgctcgaact
     1921 cgacgccgcg cggatcgccc ggatcaccga gaacagccgg cggctgggca tgacgatcat
     1981 cgttccgaaa cagggcaacg cggcgaacta cgcggaggag ggcttcgacc gggtattgat
     2041 cgacgcacca tgctccggcc tgggcgtctt gaggcggcat cccgacggca ggtggagcaa
     2101 gagcgagggg gcggtccggg aacgagcggc gttgcagcgg cggattcttg aaaactgtgc
     2161 gagactcctc cggtcgggcg gcgtgcttgt ctacgccacg tgttccacgg agccggagga
     2221 aaatgaagat gtcatcgagg catttcttgc cggccccgga agcggatttg tcgtcgctga
     2281 tccccggccc tatctgcccg ggagggcctc cgaactggtc gatggaaaca attttctcca
     2341 tacttatccg aaagcgacgg agatggacgg gttcttcggg gttcggatgg tgaagaagaa
     2401 atgatgcagg agacaggagt caggagccag taggcaaaaa gcagaattca ggctaggagg
     2461 tcttctgact tctgactcct tgcgcttagc acttgcatct gccttgaact atggtacaat
     2521 acgggctctg tcatgatgcg gttttttaaa ggcctcggga tct
//
LOCUS       DASSLD010000536         3936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1217192, whole genome shotgun sequence.
ACCESSION   DASSLD010000536 DASSLD010000000
VERSION     DASSLD010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217192"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1006)
                     /locus_tag="VFK23_09690"
     CDS             complement(188..1006)
                     /locus_tag="VFK23_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319396.1"
                     /translation="MIPVSEVGLIAKAHGAPVWGATGASDLPGVQEEIASKAEGDPLH
                     TGIPRPLEKLHDGELLLLLRLPGGLPGGKVGPVIFSPSPYFSFTTGARETVALASGFS
                     FLFRVKIGNGLDTEKSKGGEMEERIIKKYGDRVGYTETELEKFHEGGHRIRQVKRLAR
                     AASRFSIQAEVVQAKHCNSGHAVGEIFILDVDGNFISKYCPKRMCVYLISQLTIPVAL
                     INERLSEELEPNDFHFMRNVKCLDAGVECYGYGEVMLKVHVIPRVKGEPSATSA"
     gene            1087..1506
                     /locus_tag="VFK23_09695"
     CDS             1087..1506
                     /locus_tag="VFK23_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET7319397.1"
                     /translation="MKSFRKELWFDIPGRRAFVNITPQVEICLRESGIKEGLLLCNAM
                     HITASVFINDDESGLHGDYERWLERLAPHEPVSQYLHNRTGEDNGDAHLKRQVMGREV
                     VVAITGGKLDFGPWEKIFYGEFDGGRRKRVLVKVIGE"
     gene            complement(1627..1703)
                     /locus_tag="VFK23_09700"
     tRNA            complement(1627..1703)
                     /locus_tag="VFK23_09700"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1667..1669),aa:Arg,seq:acg)
     gene            complement(1983..>3936)
                     /locus_tag="VFK23_09705"
     CDS             complement(1983..>3936)
                     /locus_tag="VFK23_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET7319398.1"
                     /translation="PVTGVTHYSINIQPFQDRLHPDLGLTTLWGYNPYIPLGGGSQAQ
                     KHLGGIIVAQRGTPIQITFTNSLPPTHIIPIDTTVMGGDLGANRASVHLHGGFVPWIS
                     DGGPHAWFDPNGNAGPSFLNNLVLNPGAQAGDAEYYYPNDQSARMVWYHDHAWGNTRI
                     NAYAGIASAYLIRDGFELNLQNQGLPPFIEASVLGGTTVLELPIVVQDKIFVGPDILT
                     ADPTWPGPTTLGSLWYAHVYDTDQYGKLGSAPAGPPPNPSAVPEFFGDTMLANGTVYP
                     EVTVEPRRYRFRILNACNARFLNLQLYLDDGSPNGITLDRKGNPLNQPALNAAASNPK
                     GKPTGNFLVLGTEGGFLPSPAFVPSNLPFNPAALGGSLVLAPAERADVLVDFSPHAGQ
                     NLILYSDAPAPFPDGDPSNDYFPGFNTHKNPINALTSPGSGPNTRVILRFKVGKSVSS
                     TDKALTITRATNLTAGNDSFLVPLGVTALPPGVPVRQLTLNETFDEHGRLIQLLGTDV
                     FNAATGSFGKGYMDATTETPSANATEIWQIANLTGDTHPIHFHLVNVQIISRQAFSVS
                     SYKGTPNVKGAPVPPPPQDRGWKETVRMNPGEVTTVIMQFKLPANPPNVPAIPVSPRT
                     GGFEYVWHCHILEHEEHDMMRPLVVT"
BASE COUNT          814 a         1118 c         1162 g          842 t
ORIGIN      
        1 gatgtcctcc tttctgtttt tattatcagg caatctaaca acggacgaaa cggggcacca
       61 ccgggaggaa cccaactccc tgccttgact attccttcgt atctataaac agtgtgaacc
      121 taacgatccg gctaagctgc gggcactcaa gacgttcgcc aagtgagccg tcaacttcag
      181 cctgttatta ggcactggtc gctgatggct ctcccttaac ccttggaata acatgcactt
      241 taagcataac ttctccataa ccgtagcatt cgaccccggc gtcaaggcat ttcacgttcc
      301 gcatgaaatg aaaatcgttg ggttccaatt cttcgctcag ccgttcgtta ataagggcca
      361 cgggaattgt caactgggat atcaaataaa cacacattct ctttggacaa tatttcgaaa
      421 tgaaattgcc gtccacatca agaataaata tttctccgac tgcatgcccc gaattgcagt
      481 gctttgcttg caccacctct gcttgtatgg aaaagcgtga tgccgccctg gcaagccgtt
      541 taacctgcct gatcctgtgg cctccctcat ggaacttttc gagttcggtt tccgtgtatc
      601 cgactctatc tccatatttc ttaattattc tttcttccat ctctcccccc tttgattttt
      661 ctgtgtccaa cccattccca atctttacac ggaacaggaa ggagaagccc gaggccaagg
      721 cgacggtctc cctcgcgccg gtggtgaagg agaagtaagg ggacggggag aagattaccg
      781 ggccgacttt cccaccgggc aaaccgcctg gcaggcgaag cagtaatagt agttccccgt
      841 catgaagttt ttccaaaggt cgagggatac ctgtatggag aggatctcct tccgctttgc
      901 tcgcgatctc ctcctgcaca cccggcaggt ccgaggcgcc cgtcgctccc cacaccggag
      961 cgccgtgcgc tttcgctatg agaccgactt ccgaaactgg gatcatcacc ggctccttgg
     1021 gagaatcgtg tcctcgcgtg ggtttgtggc ccataatgtt tctatcttac cccggggggg
     1081 tggaggatga agagcttccg gaaggagctt tggttcgaca ttccgggaag acgggcattc
     1141 gtcaacatca cacctcaagt cgagatatgc ctccgagaga gcggaatcaa ggaaggactt
     1201 ctgctttgca acgctatgca tatcaccgca tccgtcttca tcaacgacga cgaaagcggt
     1261 ctccacggag attatgaacg ctggctggag cggctcgcac cccatgagcc ggtctcgcaa
     1321 tacctccata accggacggg agaggacaac ggggacgcgc acctgaagcg ccaggtgatg
     1381 gggcgggagg tggtggtggc gatcacgggc ggcaagctcg acttcggccc gtgggagaag
     1441 atcttctacg gcgagttcga cgggggtcgg cgaaagcggg tactggtcaa ggtcatcggc
     1501 gagtgaggct tcgaacccat cggtcagttc gttaagctta aatagggcaa acatcacacc
     1561 ctcacaatca cttcacattc gaacgaaaaa agggctacgg ccgtaaccgc aaccccttaa
     1621 attatctggc gcgccctaga ggattcgaac ctctggccac ttgattcgta gtcaagtgct
     1681 ctatccagct gagctaaggg cgcgtctgtg aggaaagtca ctatactgca agggaaatcc
     1741 cgcgtcaaca gctcctctac gaaaccccat tttcaccccg aaatagccgg ttgtttgcgc
     1801 ggggtttgcg gtccatccca cgccgcctcc gcgaatcagc ctgaaaagca ttaacacatc
     1861 tcggaccggc gtggtcaggc aagaaaggag gagaaaaaag ccccggcgtt tggcgccggg
     1921 gcggttccgg ttgaagtcat gttttcctct ttttccgcca gcgcgcccgc ttgcgcagac
     1981 ggtcaggtga ccaccaaggg acgcatcatg tcgtgctcct cgtgctccag gatgtggcag
     2041 tgccacacgt actcgaaccc gcccgtcctc gggctgacag ggatggctgg gacgttcgga
     2101 ggatttgcgg ggagcttgaa ttgcatgatc accgtcgtca cctcgccggg gttcatccgg
     2161 acggtctcct tccagcctcg gtcctgcggc ggaggcggaa cgggcgcacc cttgacgttc
     2221 ggcgttccct tgtaggagct caccgagaag gcctgacgcg agatgatctg cacgttgacc
     2281 aggtggaagt ggatcggatg ggtatcgccc gtcaggttgg cgatctgcca gatctcggtg
     2341 gcattcgccg agggcgtctc cgtcgtcgca tccatataac ctttgccgaa agaccccgtt
     2401 gccgcgttga acacgtccgt gccgaggagc tggatcagac gaccatgctc atcgaacgtt
     2461 tcgttcaggg tcaactgccg gaccggaact cccggaggaa gcgccgtgac cccaaggggc
     2521 accagaaacg aatcgtttcc cgcagtgagg ttagtggccc tcgtgatggt gagcgctttg
     2581 tccgtcgatg agaccgactt gcccaccttg aagcgcagga tgacccgtgt gtttggaccg
     2641 gaacccggcg aggtgagggc gttgatcgga ttcttgtggg tgttgaagcc cgggaaatag
     2701 tcgttgctcg ggtcgccatc cgggaacggc gccggcgcgt cgctgtaaag gattaggttc
     2761 tgaccggcgt gcggcgaaaa gtccacaagc acgtctgcgc gctctgccgg cgcaaggacg
     2821 agagagcccc caagtgccgc aggattgaac ggaaggttcg acggcacaaa cgccgggctg
     2881 ggcaggaacc cgccttcggt cccgagcacc aggaagttcc ccgtcggctt cccctttgga
     2941 ttcgaggccg ccgcgtttag agccggctgg ttcaggggat tccccttcct gtcaagggtg
     3001 atcccgttcg ggctcccgtc atccaaatac aactggaggt tcaggaatcg cgcgttgcag
     3061 gcgttcagga tacggaaccg gtatctccgg ggctcaacgg tcacctccgg gtagaccgtt
     3121 ccgttcgcca gcatggtgtc gccgaagaac tcggggaccg ccgagggatt cggcgggggg
     3181 cccgcggggg ccgatcccag tttcccgtat tggtccgtgt cgtagacgtg ggcgtaccac
     3241 aaactgccga gcgtcgtcgg tccgggccag gtgggatccg cggtgaggat gtcggggccg
     3301 acgaaaattt tgtcctggac aacgatgggc aactcaagga ccgttgtacc gccgagaacg
     3361 ctcgcttcga tgaaaggcgg aagcccctgg ttctggaggt tcaattcgaa accgtcgcgg
     3421 atgagatacg ctgaggcgat ccccgcgtaa gcgttgatcc gggtgttgcc ccacgcatgg
     3481 tcgtggtacc acaccatccg ggcgctttgg tcattgggat agtaatattc ggcatcgccg
     3541 gcctgcgcgc ccgggttcag caccaagttg ttcaggaagc tgggcccggc gtttccgttc
     3601 ggatcaaacc aggcgtgcgg acccccgtcg ctgatccagg gaacaaaccc gccatggaga
     3661 tgcaccgagg cgcggttcgc gcccaggtcc cctcccatga ccgtcgtgtc gatgggaatg
     3721 atatgagtgg gtggaaggga atttgtgaat gtgatttgga tcggggtgcc tctttgcgcg
     3781 acgatgatgc cgccaagatg cttctgcgcc tgagaacccc cgccaagcgg tatgtacgga
     3841 ttgtaccccc acaatgtcgt gagaccgagg tcggggtgaa gtcgatcttg aaatggttgg
     3901 atattgatcg agtaatgggt aactcccgta acgggc
//
LOCUS       DASSLD010000537         2826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1282629, whole genome shotgun sequence.
ACCESSION   DASSLD010000537 DASSLD010000000
VERSION     DASSLD010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282629"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VFK23_09710"
     CDS             <1..605
                     /locus_tag="VFK23_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7319399.1"
                     /translation="RRYPVLNRENELVGIITKGSIVEGLLRKLESEYEKAESKAVVTR
                     QRPTMRKFFEDVSADEISFTFKYNVAGQSLKDAGKAASELKNALKKLGVCPPLLRRIG
                     VATYEAEINLASYTQGGEIRAQVEKWGITVEAVDSGPGIPDVELAMQPGYSTAPEWVR
                     ELGFGAGMGLVNIKKCADEMNLKSAVPDGTHLTMQFYYTS"
     gene            612..956
                     /locus_tag="VFK23_09715"
     CDS             612..956
                     /locus_tag="VFK23_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HET7319400.1"
                     /translation="MMKLSDVVKEFGLEVKAGASSLDREVTGGYASDLLSDVLAHADD
                     GVLWVTLHIHQNIVAVASHKGLSGIILVQGRVPDKDTVEKAEEESIPIMVSGLPAFEL
                     VGRLYKVGIRGA"
     gene            946..1692
                     /locus_tag="VFK23_09720"
     CDS             946..1692
                     /locus_tag="VFK23_09720"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET7319401.1"
                     /translation="MALRRIKADFHVHTCLSPCSSLDMSPREVVNAALQKGIDLIGVC
                     DHNSAENVLAVKKAGRRNGLMVLGGMEVASSEEAHILVFFDDDEDLLNFQDYIYRHLE
                     GRNDEHSFGLQVIADDEDGVTGFCDRLLIAATSLPAETISAVAREAKGESLVIAAHVD
                     REAFSIIGQLGFIPPGLGLDGVELSGRMTAAALAARYPECGNFPVVTGSDAHGPGDIG
                     KRYTLLTVEDGSIAEIKKALTGRDGRKAEC"
     gene            1697..2224
                     /locus_tag="VFK23_09725"
     CDS             1697..2224
                     /locus_tag="VFK23_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7319402.1"
                     /translation="MDDLSLHILDIAENSVRADARNIEIIISREAGILRVEVNDDGRG
                     MDAATLARVRDPFFTTKRKKTGLGIPLLSQAAEQTGGNVTVDSAPGRGTKVTVAFPWE
                     HVDRPPVGSMADTLLTLIAGHPDREYIYEEREGDRSFRFDTREIKADLDGIPITEPAV
                     LAAIRGLLKENIRIV"
     gene            2236..2748
                     /locus_tag="VFK23_09730"
     CDS             2236..2748
                     /locus_tag="VFK23_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET7319403.1"
                     /translation="MSNEAAVQQTTTVETDSITPEMYRKIDAIIDKYKDKPGSLIPVL
                     QQAQDVCGYLPHAVQRYIAKGMKMSPSVVFGVATFYSFFTLVPRGKHVIRVCLGTACY
                     VKRSEEILDKIKDELDLEVGEITRDKKYSLEAVRCLGACGLAPVVVIGQDTYGDVAAT
                     KVMDIIKKYE"
BASE COUNT          622 a          787 c          926 g          491 t
ORIGIN      
        1 ttcggcggta tccggtcttg aaccgggaga acgagctggt cggcatcatc acgaagggga
       61 gcatcgtcga gggcctgctc aggaaacttg agtccgaata cgagaaggcc gagtcgaaag
      121 cggttgtgac gcgccagcgg cccacgatga gaaagttctt cgaggacgtg agcgcagacg
      181 agatcagctt caccttcaag tacaatgtcg ccggccagtc gctcaaggac gcgggtaagg
      241 cggcgagcga gctcaagaac gcgctcaaga aattgggcgt ctgcccgccg ctcctgcggc
      301 gcattggtgt cgcaacctac gaggcggaga tcaacctcgc ctcctacacg cagggcggcg
      361 agatccgcgc gcaggtggag aaatggggga tcaccgtgga ggccgtcgac agcggtccgg
      421 gaattcccga tgtcgagctt gccatgcagc cggggtattc cacggcccct gagtgggtgc
      481 gggaattggg gttcggcgcg ggcatggggc tggtgaacat aaagaaatgc gccgatgaga
      541 tgaacctgaa gtcagcggta cccgatggaa cgcatttgac gatgcaattt tactacacaa
      601 gctgacgggg gatgatgaag ctcagcgacg tcgtgaaaga gttcggcttg gaagtgaagg
      661 caggggcctc ttccctcgac cgtgaagtga ccggcggata cgcaagcgac ctcctgtcgg
      721 acgtgctggc ccacgcggat gacggcgtgc tctgggtcac gctccacatc caccagaaca
      781 tcgtggctgt cgcgtcgcac aagggactct ccggcatcat cctggtacag ggcagggtcc
      841 cggacaaaga cacggtcgaa aaagccgagg aagagagcat cccgatcatg gtgagcgggc
      901 ttccggcgtt cgaactcgtc ggcaggctct ataaggtggg catccgtggc gcttaggcgc
      961 atcaaggcag actttcacgt gcacacctgc ctgtctccct gctccagcct ggacatgtcg
     1021 ccccgcgaag tcgtgaacgc ggcccttcag aagggcatcg acctgatcgg cgtgtgcgac
     1081 cacaattcgg cggaaaacgt cctggccgtg aagaaggcgg gcaggcgaaa cggactcatg
     1141 gtgctgggcg ggatggaagt ggcgtcctcc gaagaggcgc acatccttgt gttctttgac
     1201 gatgacgaag accttctgaa ttttcaggac tatatttacc ggcacctgga gggaaggaac
     1261 gacgagcaca gcttcgggct gcaggtgatc gcggacgacg aggacggagt gaccggtttc
     1321 tgcgaccggc tcctgattgc ggcgacctcg cttcccgccg agacgatctc ggcggtggcc
     1381 cgcgaggcaa agggtgaaag cctcgtgatc gccgcgcatg tggaccgcga agctttcagc
     1441 atcatcgggc agctcgggtt catacctccc ggccttggcc tggatggcgt tgaactgtcg
     1501 gggcggatga ccgcggccgc gctggcggcc cgatatcccg agtgcgggaa cttccccgtc
     1561 gttaccggct ccgacgcgca tggccccggg gacatcggca agcgctacac tttgttgacg
     1621 gttgaagacg ggtccattgc cgagatcaaa aaggccctga cggggaggga cggcaggaag
     1681 gcggagtgct gaggccgtgg atgatctctc gctccatatc ctcgacatcg ccgaaaattc
     1741 ggttcgtgcc gacgcaagga atatagaaat tattatctcg cgggaggcgg gcattctccg
     1801 cgtcgaggtg aatgacgacg gaaggggaat ggacgccgca acgcttgcca gggtccgtga
     1861 cccgttcttc accacaaaac gcaagaaaac aggcctggga ataccgctcc tttcccaggc
     1921 ggcggaacag acgggcggaa acgtaaccgt cgattccgct ccgggcaggg gcacgaaggt
     1981 gacggttgct tttccctggg aacatgtgga caggccgccc gtcggcagca tggccgatac
     2041 gctcctgacg ctgatcgcag gccacccgga cagggagtat atctatgaag aacgggaagg
     2101 ggaccgctcc ttccggttcg acacacggga gatcaaggcc gatctcgacg ggatcccgat
     2161 caccgagccg gcggtgctgg cagcgatcag gggcttgctg aaggaaaata tacggatcgt
     2221 ttaacgaggt agaccatgag caacgaagcg gcagtacagc aaacaactac ggtggaaact
     2281 gactccataa cgccggagat gtaccggaag atcgacgcca tcattgacaa gtacaaggac
     2341 aagccagggt cgcttatccc ggtgctccag caggcgcagg acgtctgcgg ctacctgccg
     2401 cacgccgtgc agcgctatat cgcgaagggg atgaagatgt cgccgagcgt cgtgttcggc
     2461 gtggccacgt tctattcctt ctttaccctg gtcccgcgcg gcaagcacgt gatacgcgtc
     2521 tgcctcggca cggcctgcta cgtcaagcgc agcgaggaga tcctcgacaa gatcaaggac
     2581 gaactggacc tcgaggtcgg cgagatcacc cgggacaaga aatactccct cgaagccgtg
     2641 cggtgcctcg gcgcctgcgg cctcgcgcct gtcgtggtca tcggacagga cacctacggc
     2701 gacgtggcgg cgaccaaggt gatggacatt atcaaaaagt acgaatagcc tctgaattcg
     2761 gaatgcggaa tgtaacggcg ggggtattat ggctaaactg acgattgcgg atttaaaaaa
     2821 gatcaa
//
LOCUS       DASSLD010000538         4011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1432494, whole genome shotgun sequence.
ACCESSION   DASSLD010000538 DASSLD010000000
VERSION     DASSLD010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432494"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1423
                     /gene="aroA"
                     /locus_tag="VFK23_09735"
     CDS             116..1423
                     /gene="aroA"
                     /locus_tag="VFK23_09735"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET7319404.1"
                     /translation="MSDLVIRPSRGLRGEIELPGDKSISHRSVMFAAIAEGDTRITGF
                     LTGADTMNTAKAVRMLGAEVEGIGSQNLVVHGKGLHGLTEPTCVLDLGNSGTGVRLLA
                     GLLAGQDFFSVLTGDQYLRKRPMARIVEPLRQMGAGIDGRSGGRLAPLAIRGSRGMRA
                     IDYQSPVASAQVKSALLLAGLFANGATSVSEPSKSRDHTERLFRFFGVTVKEEGNRVT
                     VPGLQRLRAPGPLAIPSDISSAAFFMVAASIVPGSDLLIRNVGVNPTRTGVIDVLSAM
                     GADIVLENGREEAGEPVADIRVRHVRLRATKISGDVIPRAIDEIPVLCVAASYAEGTT
                     VIRDAAELRVKESDRIATMAAELRKMGVVVTESPDGMEITGQETLTGAVCESHGDHRI
                     AMSMAVAGLAARGDTSVRDAGWIETSFPGFERLLRKAAYGKDA"
     gene            1541..2221
                     /gene="cmk"
                     /locus_tag="VFK23_09740"
     CDS             1541..2221
                     /gene="cmk"
                     /locus_tag="VFK23_09740"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET7319405.1"
                     /translation="MNRKKLVIAIDGPSGAGKSTIAKLLASRLGYVYIDSGAMYRAVG
                     WKAKREGIDPADEGRLADLCRSTEVTIRLDGRDPRFYVDGIDVSGEIRTPEMGMMASA
                     VSKARAVRERLLVLQRDLGKNGGVVMDGRDIGTVVFPGAGVKFYLDASAEERGRRRYL
                     ELKAKGMEVDLEQITREIRDRDRQDSTRSLAPLRRADDAVLIDSTKLTIDEVLEKMLS
                     EIARIKNR"
     gene            2391..3467
                     /locus_tag="VFK23_09745"
     CDS             2391..3467
                     /locus_tag="VFK23_09745"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET7319406.1"
                     /translation="MSKTYKIALIPGDGTGPEVVREAVKVVNAAAAKGGFKLAFTEFD
                     FGAARYLKTGETLPDSAIGEFKKFDAMFLGAIGHPDVKPGILETGILLKIRFALDQYI
                     NLRPVKLYPNVETPLRDKGPEHIDFVVIRENTGGIYTGHGGATRVGTIDEVATQLMVY
                     DRRTVDRCLRYAFELKKKRNTKDPRYAAKPITLIHKRNVLTHCGDLWYRAFEEMGAKD
                     YPELKRDYNHVDAANMWFVKNPEWFDVAVTENLFGDIITDLGAMIQGGLGVASGGNIN
                     PEGVSMFEPMGGSAPKYAGQNVINPMAAIGAAMMMLDSLGEAKSAQAIENAMIATMKK
                     MKSQAAGKMGYSTTEVGDMVASLV"
     gene            3520..>4011
                     /locus_tag="VFK23_09750"
     CDS             3520..>4011
                     /locus_tag="VFK23_09750"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019601546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET7319407.1"
                     /translation="MRVGFIGWRGMVGSVLMGRMNEEKDFDLIEPVFFTTSNVGGKGP
                     DIGKNVPALKDAKSLDELKKLDAIVSCQGGEYTNEVYPKLRSAGWKGYWIDAASTLRM
                     TSDSIIILDPVNMNVIKDGLAKGIKNYIGGNCTVSLMLMALGGLYERDLVEWMSAMTY
                     QAAS"
BASE COUNT          870 a         1136 c         1293 g          712 t
ORIGIN      
        1 cgagcggtac caggcgtcgc ttgccaggat caagaaagcg atccagagcc gggacggcgt
       61 cagactggaa aagcttttcc attcggcaag cgtacttcgc agaggtttgg gctaaatgtc
      121 cgacctcgtg atacggcctt cacgcggact tcgcggagag atcgagttgc cgggcgacaa
      181 gtccatctcg caccggtccg tcatgtttgc cgccatcgcc gagggcgaca cgcgcatcac
      241 cgggtttttg accggcgcag acaccatgaa cacggcgaag gccgtgcgga tgctcggggc
      301 cgaagtcgag gggatcgggt cccagaacct cgtcgtacac gggaaagggc ttcacggcct
      361 gaccgagccg acctgcgtgc tggacctcgg gaactcgggc accggcgtgc ggcttctggc
      421 gggtctgctc gcggggcagg atttcttctc cgttctcacg ggggaccagt atctgcgaaa
      481 acggcccatg gcgaggattg tggagccgct ccggcagatg ggcgccggga tagacggccg
      541 ttccggcggc aggcttgccc cgctcgctat tcgcggaagc aggggtatga gggccatcga
      601 ttaccaaagc ccggtagcca gcgcccaggt gaagtcggcg ctgcttctgg ccggcctctt
      661 tgcgaacggc gccacatcgg tcagcgaacc gtccaagtca cgggatcaca cggagcgact
      721 gttccggttc ttcggcgtta cggtaaaaga agaggggaac cgggtgaccg tgcccggact
      781 gcagcggctg cgcgccccgg gaccgctcgc cataccctcc gacatttctt cggcggcgtt
      841 cttcatggtc gccgcctcga tcgttcccgg gtcagacctc ctgataagga acgtcggcgt
      901 caatcccacg cggaccggcg tcatcgatgt cctcagcgca atgggggccg atatcgtcct
      961 cgagaacggg cgggaagagg cgggtgagcc cgttgccgac atccgcgtga gacatgtcag
     1021 gctcagggcg acgaagattt cgggagacgt catcccgcgg gcaatcgatg aaatcccggt
     1081 cctgtgcgtg gcggcctctt atgccgaggg cacgaccgtg atcagggacg ctgccgaact
     1141 ccgggtcaag gagtccgacc ggattgcgac gatggccgcc gagctccgca agatgggcgt
     1201 cgtcgtgacg gagtcaccgg acgggatgga aataaccggt caggagacgc tcacgggcgc
     1261 agtgtgcgag agccacggag accaccggat cgccatgtcc atggccgtcg ccggccttgc
     1321 cgcccggggg gacacgtccg tgcgcgatgc ggggtggatc gaaacgtcct ttccggggtt
     1381 cgaacggctg ttgaggaaag cggcgtacgg aaaggacgcc tagcaaaatc cagaaggtaa
     1441 gatggtaaga aggtgagcaa aatcttgtgc ctaccctcta accctctcac cttcctacct
     1501 tcgagagtta acgccatgcg atgttcggcg tataagacta atgaatagaa agaagttggt
     1561 catcgccatc gacgggccgt cgggcgcggg aaaaagcact atcgcgaaac tgcttgcctc
     1621 gcgactcggc tatgtctata tcgacagcgg cgccatgtac cgcgccgtgg gctggaaggc
     1681 gaagcgggag ggcatcgatc cggccgatga aggcaggctc gccgatctgt gccggagtac
     1741 cgaggtaacg atcagactgg acggccgtga tccccggttt tacgtggacg gcatcgacgt
     1801 cagcggtgaa attcggacac cggaaatggg aatgatggca tcggcggtct cgaaagcccg
     1861 cgccgtccgg gagcggctgc tcgtcctcca gcgggatctg ggcaaaaacg gcggcgtggt
     1921 catggacgga agggacatcg gcaccgtcgt ttttcccggc gccggggtga aattttatct
     1981 ggatgcgtca gccgaggagc ggggaaggcg ccggtatctc gagctgaagg cgaaggggat
     2041 ggaggtcgac ctggagcaga tcacccggga gatcagggac cgggaccgcc aggacagcac
     2101 gcgctcgctc gcgccgctcc ggcgggccga cgatgccgtc ctgatcgatt ccaccaagct
     2161 gaccatcgac gaagtgctgg agaagatgct gtctgagatt gctcggataa aaaatagatg
     2221 accgccaagg tctatcacca atgccagaaa ggaccctgta aaaccgaatt gtcatttccg
     2281 aatgattcta tcgggaatcc attttgaatt tggcctttta taaatccaat ggattcccgc
     2341 ttccgcggga atgacaacct tagagtctta ttttggagga ttcatcatct atgtccaaga
     2401 cctacaagat cgcattgatc cccggcgacg gtaccgggcc cgaagttgtt cgggaagcgg
     2461 taaaagtcgt caatgccgcc gcggccaagg gcggattcaa acttgcgttc accgagttcg
     2521 acttcggcgc ggcacggtat ctgaaaaccg gagagacgct tcccgacagc gcgatcggag
     2581 aattcaaaaa gttcgacgcc atgttcctgg gcgccatcgg gcatcccgat gtgaagcccg
     2641 gcatcctcga aaccgggatc ctcttgaaga tccgcttcgc cctcgaccag tacatcaatc
     2701 tccggccggt gaagctctac cccaacgtcg agacgccgct ccgggacaag ggcccggagc
     2761 acatcgattt cgtggtcatc cgcgagaaca cgggcggcat ctacacgggc cacggcgggg
     2821 ccacgcgtgt cgggacgata gacgaagtcg ccacgcaact catggtctac gaccgccgaa
     2881 ccgtcgaccg gtgcctcagg tatgccttcg agctgaagaa gaagcgcaat acaaaggacc
     2941 cgaggtatgc cgccaagccg atcacgctca tccacaagcg gaacgtgctt acccactgcg
     3001 gagacctctg gtaccgcgcc ttcgaggaaa tgggcgcgaa ggactatccg gaactcaaac
     3061 gcgactacaa ccacgtagat gcggcgaaca tgtggttcgt caaaaacccc gaatggttcg
     3121 atgtggcggt cacggagaac ctgttcggcg acatcatcac cgacctcggc gccatgatcc
     3181 aggggggcct gggcgtggcc tcgggcggaa acatcaaccc cgagggcgtt tcgatgttcg
     3241 agcccatggg agggagcgcg ccgaagtacg ccggacagaa cgtgatcaac cccatggccg
     3301 caatcggcgc tgcgatgatg atgctcgatt cgctcggaga agcgaaatcg gcgcaggcga
     3361 tcgagaacgc aatgatcgcg actatgaaga agatgaagag ccaggccgcc gggaagatgg
     3421 gatactcgac taccgaagtg ggggacatgg tggcatcctt ggtttaagag ttcggagttt
     3481 agagttcgga gtaaatacga aagcaggagt aagattatga tgcgggttgg ttttataggc
     3541 tggcgcggca tggtgggctc ggtgctcatg ggccgcatga atgaggaaaa ggacttcgat
     3601 ctcatcgaac cggttttctt cacgacttcg aacgtgggcg ggaaggggcc ggatatcggc
     3661 aagaacgtcc cggcgctcaa ggacgcgaag agcctcgacg agctgaaaaa actcgatgcg
     3721 atcgtctcct gccagggcgg agaatatacc aatgaggttt atccgaagct ccgctccgcg
     3781 ggatggaagg gctactggat cgacgcggcg tcgacccttc gcatgacttc cgattccatc
     3841 atcatcctcg atccggtgaa catgaacgtg atcaaggacg gcctggcgaa gggcatcaag
     3901 aactacatcg gcggcaattg taccgtgtcg ctcatgctca tggcgctggg cggactgtac
     3961 gaacgcgacc tcgtggaatg gatgagcgcc atgacctacc aggcggcctc g
//
LOCUS       DASSLD010000539         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1510837, whole genome shotgun sequence.
ACCESSION   DASSLD010000539 DASSLD010000000
VERSION     DASSLD010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510837"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFK23_09755"
     CDS             <1..237
                     /locus_tag="VFK23_09755"
                     /inference="COORDINATES: protein motif:HMM:NF036497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET7319408.1"
                     /translation="PYTKRGKVAGFRITRISPGSIYEKIGLQNGDVLLRVNTQNLDNP
                     AKLFSLYKEMRNKRHISLLLSRNGQDRTFQYDIR"
     gene            424..1344
                     /locus_tag="VFK23_09760"
     CDS             424..1344
                     /locus_tag="VFK23_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7319409.1"
                     /translation="MVRGIDKSNIFRDNEDKAHFLERLGKNVKEGQCSVYAWVFMSNH
                     VHILFRSGKDGISTVMRKLLTGYAQYFNRKHGRSGHLFENRFKSILCDEDNYLLALIR
                     YIHLNPVRANIVTTIEQLDRYPWSGHRTLIGKTKLPWMDTDHVLTEFGSTRGRAASEY
                     RRFMREGLGQGRIPELTGGGLTRSKGGWSQVLSARQSGHREEYDERILGTGDFVQAIL
                     KEAEEKTTRQLKLRRSGKTFAGIIDEECRKGSISQEELKGGGRRRKISIIRATIAKRG
                     LEELGLSLAEIARHVGVNTSSIRKAVLRLE"
     gene            1692..2336
                     /locus_tag="VFK23_09765"
     CDS             1692..2336
                     /locus_tag="VFK23_09765"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7319410.1"
                     /translation="MKIVIACESSFFCEGMATLLQREKDIEIVGKINHASRIMKSLDR
                     NPDVIILDPLLFEPEKLTGVIDELKLKAPRTRIILVFSEQEMSDTSLMRYMMRGVDGY
                     IKRAEKLKHVIEAIRTVHAGSIWAERKLLHKFVRDSPLLTLDLDSRLSKMENPLTKRE
                     KDIVTLLVHGLSNKRISSRLHISEKTVKTHLNNVFKKMNVTSRTQVISSLVYGQ"
BASE COUNT          673 a          635 c          660 g          538 t
ORIGIN      
        1 ccatacacga agcgcggcaa ggtcgcgggg tttcggatca ccaggatttc tccgggaagc
       61 atctacgaaa agatcgggct tcagaatggt gatgtgctgc tccgggtcaa tacgcagaac
      121 cttgacaacc ctgcaaagct ctttagtcta tataaagaga tgagaaataa acggcatatc
      181 tccctcctcc tttcccgcaa cggccaggac cggacgtttc aatacgatat tcgttaaatt
      241 ccgagaacta aatctattca attgtcgtct tattttgaac agaagattta ggggacgttg
      301 agaagattta ggggacgttg tgtcctacgt gtcctattgc gtgacaggtc atgtcaattt
      361 ctatagacta accccatgcc tcgtcaagcc cgcttagatg ttcccggcgc cttgcatcat
      421 atcatggtgc gggggatcga caaatcgaac atattcagag acaatgagga caaagcccat
      481 ttcctcgaac gcctcggtaa gaacgtcaaa gaaggacaat gttcggttta tgcttgggtc
      541 ttcatgagca accacgttca cattctgttt cgcagcggca aagacggcat atcgacggtc
      601 atgcgcaagc ttcttaccgg gtacgcgcag tatttcaatc gcaaacatgg ccggagcggt
      661 caccttttcg aaaacaggtt caagtccatt ctctgcgatg aagataacta tctcctggca
      721 ctcatccgct acattcactt aaatcccgtc cgggccaaca tcgtcacgac catcgaacaa
      781 ctggatcgct atccttggag cggccaccgg acgctcatcg gaaaaacaaa gctgccctgg
      841 atggacacag atcatgtatt gaccgagttc ggttcaaccc gaggaagggc ggcaagcgag
      901 tatcggcgct tcatgcggga aggtctcggc cagggtcgaa ttccagagct taccggcgga
      961 ggtcttacaa gaagcaaagg aggatggtcc caggtcctct cagcacggca gagcggccat
     1021 agagaggaat acgacgagcg catactcggg accggcgatt ttgttcaggc tatactcaaa
     1081 gaagccgaag aaaaaacgac acggcagtta aaactacgtc gaagcggcaa gacatttgcc
     1141 gggattatcg acgaagagtg caggaaagga tcgataagcc aggaggaact caaagggggc
     1201 ggccgccgga gaaagataag catcatccgg gcaacgatag cgaagcgcgg tctggaggaa
     1261 ttgggtctgt ccttggcaga aatcgctcga cacgtgggtg tgaacacgtc cagcatccgc
     1321 aaggcggtct tacgattgga atgagagtag tatggctagc tgggggtgaa atcagaaagg
     1381 acacgtagga cacaacgtcc ccaaagacaa agatcccttt gctcgcatga atgggccgtt
     1441 gaaaggaggc acgtatgaaa aagatcgttc cgtgcctgtg gttcgcacgg gcgttgtcgt
     1501 cagtgtctac cgcagcgcag gaagcctgaa aacgggctcg ttcgccctcg attgagcggt
     1561 tgccgcatga gcattggccg cgggcgcagc aagtcctgca atccgtgtct gaattgtcta
     1621 taatagtaaa aaatgtggcg atgactgacg ccgtttctcc tacgacggta tgaggaaggc
     1681 ttgtgaaaac cgtgaaaata gtaattgcat gcgaatcttc gttcttctgc gaaggaatgg
     1741 cgacgctgct ccagcgcgag aaggacattg agatcgtcgg aaaaataaac cacgcctcgc
     1801 ggatcatgaa gagccttgac cggaatcccg acgtcatcat tctggaccct cttctcttcg
     1861 agccggagaa attgacgggc gttatcgacg agctgaagct caaggccccg cggacaagaa
     1921 ttatcctggt attctcggaa caggagatgt ccgacacgtc cctcatgcga tacatgatga
     1981 ggggggtcga cggctacatc aagcgcgcgg aaaaattgaa gcacgtcatc gaggcaatac
     2041 ggaccgtgca tgcgggaagc atctgggccg aaaggaaact cctccataag ttcgtccggg
     2101 actctcccct cctgacgctg gaccttgatt cgaggctctc aaagatggaa aaccctctta
     2161 cgaagcggga aaaggacatc gtcaccctgc tcgttcacgg actgtccaac aaacgcattt
     2221 cttcgcggtt gcatatcagc gaaaaaaccg tgaagaccca tctcaacaac gtcttcaaaa
     2281 aaatgaacgt gacgagcagg acccaggtta taagttccct cgtctacggg cagtgagcgc
     2341 aatttccccc actccgtaaa attccaccct tcgaagtatt tcatccgctc aaaatccatc
     2401 ctttgatgga ttgccgtttc cgacaacgca gaatatcctt tttcgcatca gcttttattc
     2461 tcataagtgc tccggagaaa gttgttcacg agatgcaaga actgcg
//
LOCUS       DASSLD010000540         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1054045, whole genome shotgun sequence.
ACCESSION   DASSLD010000540 DASSLD010000000
VERSION     DASSLD010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054045"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VFK23_09770"
     CDS             <1..561
                     /locus_tag="VFK23_09770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7319411.1"
                     /translation="DGNFRIGFVGGFTREKNVACLLRAFAALSRREIPARLFLVGDGA
                     EKESLKSLARELDLADTVHFLGYQDNPYAFLKEFDVLVVPSYYETFSYVILEAIACGV
                     PVISSKWPSCEYLYRDGENCLLFPVNDHNALSQAIERMINDKQLRTRLVKNGQALLDK
                     CNVDTVIKQYDEAIERVLGGRGSGRS"
     gene            597..1397
                     /locus_tag="VFK23_09775"
     CDS             597..1397
                     /locus_tag="VFK23_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7319412.1"
                     /translation="MKKIYTVRNVFQQQTHLWDKHTIAEEVAACSRRNIRVTFLKYLP
                     KDEPILEAGCGLGAWVIYLSEKGYDIAGIDNNEAVIEQLKAWNPSLKVMYGDIRKLPY
                     DDNSLGAYISLGVVEHFEEGCGEAMKEAYRVLRPGGLVFLTVPMNNIFRKAFAHHIRS
                     LYLFWSRLRNNPAFFAEYRYSRRETEELLKKYGFDPVFSTWDDFDRADMSLGLWADFP
                     PLQSPKLYRLNLPGRLAAVVLNSTSRWTSAAGVFCLARKRREESNEFS"
     gene            1481..>2046
                     /locus_tag="VFK23_09780"
     CDS             1481..>2046
                     /locus_tag="VFK23_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET7319413.1"
                     /translation="MTRKTHTDCVHFNGYKPCRPHKLKSSICEGCEDFSPVGFRVLIL
                     KIGAAGEVIRNTPLLHRIKSLYADKNVEITWMTDFPDFVPRSLVKRVLKYDWKSVQML
                     LEEEFDLLLSLDKEHDVCALANRIQAKVKKGFLLDKRGRIVPADDDARRKWLTGVFDD
                     LMKENRMHYVEELFEICGWQWEGEKYIL"
BASE COUNT          478 a          558 c          571 g          439 t
ORIGIN      
        1 gacggcaatt tcaggatcgg cttcgtaggc ggatttacgc gggaaaagaa cgtcgcgtgt
       61 ctgctccggg cttttgccgc gctcagccgc agggaaattc ccgcgcgcct gtttctcgtg
      121 ggtgacggcg ctgagaaaga gtccctcaaa agccttgcga gagagcttga ccttgcggat
      181 acggttcatt ttctcggata tcaggataat ccctatgcct tcttgaagga attcgacgtg
      241 ctcgtggtgc cctcgtacta cgaaaccttc tcctatgtca tccttgaggc gattgcctgc
      301 ggcgttccgg tgatttcgag caagtggcct tcctgcgagt atctgtaccg ggacggcgaa
      361 aattgccttc tcttccccgt taacgaccat aacgcgctgt ctcaggcgat agagagaatg
      421 atcaacgaca agcaacttcg gaccaggctg gtaaaaaacg gacaggctct cctcgacaag
      481 tgcaacgtcg acaccgtcat caaacaatat gacgaggcaa ttgaacgcgt gctgggcggt
      541 cgcgggtccg gacgatccta ggcagcatcc cccgggatgg aaagagggta tgcacaatga
      601 aaaaaatcta taccgtcagg aacgtcttcc agcagcaaac gcacctgtgg gacaaacata
      661 ccatcgccga ggaagttgcg gcctgttccc ggcggaacat cagggtaacg tttttaaaat
      721 acctgccgaa agacgaaccc attctcgaag ccggctgcgg tctcggcgcc tgggtgattt
      781 acctgagcga gaagggctat gacattgccg ggatcgacaa caacgaggcg gttatcgagc
      841 agctcaaagc ctggaaccct tccctcaagg tcatgtacgg ggacattcga aagttgccct
      901 atgacgacaa ctcgctcggc gcctacatct cattgggcgt ggtcgagcat ttcgaggagg
      961 gctgcggcga agcgatgaag gaggcctacc gggtgctgag gccggggggc ctcgtgttcc
     1021 tcacggtgcc gatgaacaat atcttccgaa aggcctttgc ccaccacatc cgctcgctct
     1081 acctgttctg gagcaggctc aggaacaatc cggccttctt tgcggaatac cggtattcgc
     1141 gcagggaaac ggaagagctg cttaagaagt acggatttga tccggtgttt tccacgtggg
     1201 acgatttcga ccgggccgat atgtccctgg gcttatgggc cgattttcct cccctgcaaa
     1261 gcccgaaact gtaccggctc aacctcccgg gcaggttggc ggcggtcgtg ctcaactcaa
     1321 cgtcccgatg gacgtcggcc gcgggggtct tttgccttgc cagaaagaga cgagaggagt
     1381 ccaatgaatt ttcttaagat ctttctctct gtctctgtgt ctccgtggcc gatctcattt
     1441 ttttacaaga acatcatttg ttaggattgg tgaactatcc atgacgcgaa aaactcatac
     1501 ggactgcgtc cacttcaacg gttataaacc gtgccgtccg cacaaactca aaagcagcat
     1561 ctgcgagggc tgcgaagatt tctcgccggt cgggttccgg gtattaatac tcaagatcgg
     1621 ggccgccggc gaagtcatcc gcaatacccc cctgctccac cggatcaaat ccctgtacgc
     1681 agacaagaac gtggaaataa cctggatgac cgacttcccg gatttcgtgc ctcgctcgct
     1741 cgtcaagagg gtgctcaagt acgactggaa gagcgttcag atgcttctcg aagaagaatt
     1801 cgaccttctt ttgagcctcg ataaggaaca cgacgtctgc gcgctcgcga acaggataca
     1861 ggcgaaagtg aaaaaagggt tcctcctcga caaacggggc aggatcgtcc ccgccgacga
     1921 cgacgcgcgc cgcaaatggc tcaccggcgt tttcgacgac ctcatgaagg aaaacagaat
     1981 gcactatgtc gaggaactgt tcgaaatctg cggctggcag tgggaagggg agaaatacat
     2041 cctgga
//
LOCUS       DASSLD010000541         2581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_617820,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000541 DASSLD010000000
VERSION     DASSLD010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617820"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /gene="glyS"
                     /locus_tag="VFK23_09785"
     CDS             complement(<1..1165)
                     /gene="glyS"
                     /locus_tag="VFK23_09785"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET7319414.1"
                     /translation="MLSAPQLAERQQDISKEIVGPPKKVAFDADGKPTKAATVFAERN
                     GIPVNALAFKTTDKGEYVVARIDEKGGDTAVWLEQVLPGFILSIPFQKTMRWMDKDIR
                     FARPIHWILALWGGSVVNFELNGIRSGNLSRGHRFMSPGAFMVKDFKSYSLQTEPNSV
                     VIDPEVRKQRIVRQTRELSQAKGGTVLDDAGLVEEVANLVEYPVAVMGSFNKDYLRVP
                     KEVLITAMREHQRYFSVVASDGNLLPCFITISNTRAEDMEVVRAGNERVLSARLSDAA
                     YFFDHDVKQPLADRVEGLKKVTYQEKLGTIYDKTERVKVLAIYLSGLIKADINASGRA
                     AYLCKADLLTGVVGEFPKLQGVMGRHYALISGEDPRVADAILEHYLPRFAGDKL"
     gene            complement(1205..1330)
                     /locus_tag="VFK23_09790"
     CDS             complement(1205..1330)
                     /locus_tag="VFK23_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319415.1"
                     /translation="MAKEFLLEIGTEEIPSRFINPALEKMKELFSELLASGRGEI"
     gene            complement(1470..2339)
                     /locus_tag="VFK23_09795"
     CDS             complement(1470..2339)
                     /locus_tag="VFK23_09795"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET7319416.1"
                     /translation="MTFQDIILTLQQFWASKGCVIHQPYDTEVGAGTFHPATFLRVLG
                     PEPWKTAYVQPSRRPTDGRYGENPNRMQHYYQFQVILKPHPENVQDVYLESLVKLGIN
                     PLKHDIRFVEDDWESPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDLKPISAELTYG
                     IERLAMYLQGVNNVFDLAWTKGVKYGDIHHDTEIQFSKYNFELSDREMLFRNFESYER
                     ECEKLIKAGVVLPAYDYCLKCSHSFNLLDAAGAISVTERTGYIARVRKLARACAHAYL
                     AEREAKGFPMLKK"
BASE COUNT          583 a          735 c          689 g          574 t
ORIGIN      
        1 gcagcttgtc accggcaaaa cgcggcaggt aatgttcgag aatggcgtca gcgacccgtg
       61 ggtcctcgcc cgagataagc gcataatgcc tgcccataac gccctggagc ttgggaaact
      121 cgcctaccac gccggtaagc aggtctgcct tgcacagata ggctgccctc ccggacgcgt
      181 ttatatccgc ctttataagc ccggaaagat agatcgccag caccttcacc cgctcggtct
      241 tatcgtaaat cgtgccgagc ttttcctggt aggtaacctt cttgagcccc tcaacccggt
      301 cggcaagcgg ttgttttacg tcgtgatcga agaaataggc cgcatccgag agccgcgccg
      361 aaagcactcg ctcgtttcca gcgcgcacta cttccatgtc ctcggcgcgt gtgttgctga
      421 tcgtgatgaa acagggaagg agattaccgt cggaagccac cacgctgaaa tagcgctggt
      481 gttcgcgcat tgccgtgatc agaacctctt tcggaactct gaggtaatcc ttgttgaagg
      541 aacccatgac cgccacgggg tattccacga ggttcgccac ttcttccacg agcccggcgt
      601 catcgagcac tgtgccgccc ttcgcctgcg acagttcccg ggtctgccga acgatccgct
      661 gcttccgaac ctcgggatcg atcacgacgg agttcggctc cgtctgcagg ctataggact
      721 tgaagtcctt caccataaat gccccagggc tcatgaaacg atggccgcgc gaaaggttgc
      781 cgcttctgat gccgttcaac tcaaaattta ccacagaacc accccagagg gcaagtatcc
      841 agtgaatggg ccgggcaaag cggatgtcct tgtccatcca gcgcatcgtc ttctggaacg
      901 ggatggaaag gatgaacccc ggcagtactt gctcgagcca gaccgcggta tcgccgcctt
      961 tttcatcgat gcgggcgacc acgtactcac ccttgtctgt tgtcttgaac gcaagcgcat
     1021 taacgggaat accgttcctc tcggcaaaga ccgtcgccgc cttcgtgggc ttgccgtctg
     1081 cgtcgaaggc aaccttcttc ggcgggccga caatctcttt tgaaatgtct tgctgccgct
     1141 cggcgagctg cggcgcagag agcacaagcc ttcgaggcgt accaaaagca tggatcgtgc
     1201 cgttttagat ctcaccccgg cccgacgcca ggagctcgga gaagagctct ttcatctttt
     1261 ccagcgctgg attaatgaac cgggacggga tctcttcggt cccgatctct agcaagaatt
     1321 ccttggccaa gcaatctctc ctaacgccaa acgtaattga aaaaaagaag actggaaatc
     1381 ggaaatctta tcatgagatt ccatttccac tcaccttccc ttttccaatt ttcaatttac
     1441 gcattacatt tcggcgccta gcggcgccgt cactttttca gcatcggaaa ccccttcgcc
     1501 tcccgctcag ccaggtacgc atgggcgcag gcacgggcaa gcttccgcac gcgggcgata
     1561 tagcccgtgc gctcggtcac gctgatcgcg ccagccgcat cgagcaggtt gaacgagtgg
     1621 gaacacttga gacagtagtc ataggcaggc agtacgacgc cggccttaat gagcttctcg
     1681 cactcccgtt cgtaggactc gaaattccgg aagagcatct cccggtccga aagctcaaaa
     1741 ttgtacttcg agaattgaat ttctgtgtcg tggtggatat cgccgtactt cacgcctttg
     1801 gtccacgcaa gatcaaagac attgttcacg ccctgaaggt acatggcgag ccgctcgatg
     1861 ccgtaagtga gttcggcgga aatgggcttg agatcgatgc cgcccacctg ctgaaagtag
     1921 gtgaactggg taacttccat gccgttgagc cagacctccc atccgagccc ccatgccccg
     1981 agcgtcggtg actcccagtc gtcctcgaca aaacggatgt cgtgcttcaa gggattgatt
     2041 cccagcttaa cgagactttc aagatagacg tcctggacgt tttccggatg gggtttcagg
     2101 atcacctgga actgatagta gtgctgcatg cggttggggt tctcaccgta gcggccgtcc
     2161 gtgggcctgc gtgaaggctg aacgtaagcc gttttccacg gctcagggcc gaggacgcgg
     2221 aggaacgtgg ccggatgaaa agtccccgcc cctacctcag tgtcataggg ctggtgaatg
     2281 acgcagccct tcgaggccca gaactgctgt aaggtaagta taatgtcctg aaatgtcaat
     2341 gatttcccct gcccggaaaa atcggaaaga aggatggaaa aatagactgt tattatacaa
     2401 aaaaacctta tcttgtcaag aaaatatcgg gtaagtttta aatctgaggt ggacatgaag
     2461 tgtcctacca cgggtgggtg aaggtaccaa ctgcgtcaac ttcttgttcc atccaaacag
     2521 gtctttggtt acatggctgt acagattgca agggagcagc aaggcacact gatcatgcta
     2581 g
//
LOCUS       DASSLD010000542         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1217775, whole genome shotgun sequence.
ACCESSION   DASSLD010000542 DASSLD010000000
VERSION     DASSLD010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217775"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VFK23_09800"
     CDS             complement(<1..553)
                     /locus_tag="VFK23_09800"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7319417.1"
                     /translation="MRDVDFSAIMERMRASVRKSHDRIREALEKAEDFDFYSGEAHFT
                     GDYTLEVSPVSEGHRHLHSATGGSSPSSGDHGGRRHKSATGPDEKPRTIRGKTIILAS
                     GARPLVPQVKGIEGIEYLTNESALRLEESPESMVIIGGGYIAAEFAHFFEAMGTKVTI
                     IQRNRRLVPEEEPEVSESLRIALA"
     gene            complement(635..769)
                     /locus_tag="VFK23_09805"
     CDS             complement(635..769)
                     /locus_tag="VFK23_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319418.1"
                     /translation="MKEYDIIIVGSGSGANLIEDALEHNKTVALVDRGPAGGTCLNVG
                     "
     gene            1164..2189
                     /locus_tag="VFK23_09810"
     CDS             1164..2189
                     /locus_tag="VFK23_09810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET7319419.1"
                     /translation="MKIHNGRASLSRPKRADESAGARVLTPVLRCLSQDDKRITFNMG
                     LLMKRIILLFAIAIISLLPTEPALAVSQKAISAKAAIVMDDAGSVLFAKHPGARLAPA
                     STVKLMTAMVALDNLDPEKKVKISKNAARVRSIKPRIRADEEMTVSDLLHLALMKSIN
                     ASAVALAEATSGSEQEFVTLMNKKAAEIGAKDTLFANASGLPTKGRQYTTAGDLVLIL
                     KHALTYPLIREILGKKEYLVMTSGGRVLFLESTNNLLWRTDDMIGGKTGYTGTARHCF
                     VGAINTEKGPLFTAVLGARSRSSLWKNTLKLAEIGFQLEPEYKINEFRVIPVDDQQIE
                     PLSEVDY"
BASE COUNT          568 a          733 c          664 g          564 t
ORIGIN      
        1 gtgcaagggc gattcgaagc gactccgaga cctcgggctc ctcctcggga accagcctcc
       61 ggttccgctg gatgatcgtg accttcgtcc ccatggcctc aaagaaatgg gcgaactccg
      121 cggcgatata gccgccgccg atgatcacca tgctttcggg actttcctcg agccggaggg
      181 ccgactcgtt cgtaaggtat tcgatgccct cgatgccctt cacctgtggg acgagcggac
      241 gggctccgga ggcgaggatg atggtcttgc cccggatggt cctcggtttt tcgtccggtc
      301 cggtagccga tttatgccgg cggcccccgt ggtccccgga ggacggcgat gaacctccgg
      361 tcgccgaatg caggtgccga tgtccctcac tcacggggct tacttccagc gtgtaatctc
      421 cggtgaaatg ggcctcgcca gaatagaagt cgaagtcctc cgccttttcc agcgcctccc
      481 ggatgcggtc atggctcttt ctgaccgacg cccgcatccg ctccataatg gccgaaaaat
      541 cgacgtccct cacttcggcc gcgatgccga gcttcttcgc ttccctaatt tcaacgattc
      601 tatccgccgg atagacgagg atcttcgtgg ggattcaccc cacgttgaga caggttcctc
      661 ccgccggtcc ccggtccacg agggcgacgg tcttattgtg ctcgagcgca tcctcgatga
      721 ggttcgcccc gctgccggag ccaactatga tgatgtcgta ttccttcatg aacgtcctct
      781 ttcttccgcg tcttctttga taaaagttgc tcacgcaggc cgcaaaagaa cacggagaga
      841 actcaaaggg aaaaacctaa atcgtacatt ccacccctgt aaaagattct tcccgcgctc
      901 ccttgcgtga ttttccaagt ggtcggttca tttttcgggt cattcgcagg aaacggagtt
      961 taaccttgat tgtagcatgt tcgcgacgag agtcaatgac gcgtgaagcg tcgtcacgtg
     1021 aaagcgaatt cggtttcgtc gacccttgcc tcccttgacg gtcattcccg aatgcctcca
     1081 tcgggaatat ggtgcttagg cgacgaaaaa attgccgtcc aatgtggttt ttcttgacat
     1141 atgaaaacaa taaggttata aagatgaaaa tacacaacgg gcgggcgtcg ctttcgcgcc
     1201 cgaagagagc cgatgaaagc gctggcgcaa gggtgttgac gccggttttg cgctgtttat
     1261 cgcaggatga caaacgcata acgttcaaca tggggctgct gatgaagcgc attattctcc
     1321 tctttgctat cgcaatcatt tccctgctcc cgacagagcc ggctttggcc gtgtcccaga
     1381 aagcgatctc cgcaaaggcc gccatcgtca tggatgacgc gggttcggtg ctctttgcaa
     1441 aacaccccgg cgcacgactc gccccggcaa gcaccgtcaa actcatgacc gccatggtag
     1501 ccctcgacaa cctcgatccc gagaagaaag tgaagatcag caagaacgcc gcgagggtcc
     1561 ggtccatcaa gccgagaata cgtgccgacg aggagatgac ggtttccgac ctcctccatc
     1621 ttgccctcat gaagtccatc aacgcttcgg ccgtggccct tgccgaggcg acctccggtt
     1681 cggagcagga gttcgtgacg ctcatgaaca aaaaagcggc agagatcggg gcgaaggaca
     1741 cgctctttgc gaatgcgtcc ggtctgccga cgaaggggcg acagtatacc accgccggag
     1801 acctggtcct tatcctgaag cacgcgctca cctatcccct catccgcgag atactgggca
     1861 agaaggagta cctcgtcatg acgagcgggg ggcgggttct tttcctggaa agtacgaaca
     1921 atctcttgtg gcggacggat gacatgatcg gcggaaaaac gggatataca ggaacggccc
     1981 ggcactgctt tgtcggcgcg atcaatacgg aaaaaggccc cctgttcacc gcggtcctcg
     2041 gggcgcgttc gaggagcagc ctctggaaaa acacgctcaa gcttgcggaa atcggttttc
     2101 agctggagcc ggagtacaaa atcaacgaat ttcgcgtcat ccccgtggat gaccagcaaa
     2161 ttgagccgtt gagtgaagtg gattattaaa gcgtgttcat ttcaaaggtt cgattcggga
     2221 aatctccggc gaagtttcaa taaaaatcca ttcctgctaa ggcagaaaat caggttcctt
     2281 gtctctccac cctgtattta ctcttcacct ttccccatac acctattaaa aaggtaatcc
     2341 ccaataaaat acccgctgtg gcatctttga cacatcaccc gaaaataccc aatcgaggtg
     2401 attgatttaa tttacgaata cagctacaat tttattgtcc ctcgcgaaag tcactggtaa
     2461 accttttctc aactgcctcc tgtaatctga aaacgggcgt ccgcgcccat gcccgtcagc
     2521 aaaggagcc
//
LOCUS       DASSLD010000543         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_926926,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000543 DASSLD010000000
VERSION     DASSLD010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926926"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..786
                     /locus_tag="VFK23_09815"
     CDS             211..786
                     /locus_tag="VFK23_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HET7319420.1"
                     /translation="MKVIAFNGSARKEGNTAVLLNLVLDELKAEGIETEMYSLAGKPI
                     QGCIACMKCFEKKNRRCNVDKDIVNECIQKMIEADGILLGSPTYFADVSASMKALIER
                     CGMVGKANADLYKRKIGAAVVAVRRAGGIHVFNSLNYFFTISGMIIPGSSYWNVGIGR
                     TPGEVKGDAEGVQTMKDLGRNMAWLMKKLHA"
     gene            825..1622
                     /locus_tag="VFK23_09820"
     CDS             825..1622
                     /locus_tag="VFK23_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HET7319421.1"
                     /translation="MRTRTLLIVIVILAVSGLTMAQTGVKKRRPLPNEYGRVVLNNYS
                     EKAGLAPVVFDHWLHRAKFTCRLCHVDIAFAMKAGTTGITASDNMNGLYCGSCHSGRM
                     VQDGKRVFESCSKTYTKEDMTKCERCHSLGKNVKGEYDFAKFTARFPKERFGNGINWE
                     KAEEDGIIKLTDSIEGISIPRKKLTTPKDLALLPKQVEMPEIIFSHKKHAVWNGCELC
                     HPEIFGIKKGTTKYSMSDIFEGRYCGACHDRVAFPNLDCQRCHTRPV"
     gene            1645..1980
                     /locus_tag="VFK23_09825"
     CDS             1645..1980
                     /locus_tag="VFK23_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319422.1"
                     /translation="MAVNKDLYRTAVSLAIAIAAVVFLPSGNSPFLSPGSAHAQDDWR
                     REFDSICAKTQDADTFSPQELKELINRCDELKPRIEKLEESQRKVALKRLQMCRDLFV
                     FMLQSKENK"
     gene            1983..>2451
                     /locus_tag="VFK23_09830"
     CDS             1983..>2451
                     /locus_tag="VFK23_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HET7319423.1"
                     /translation="MRNIVPSMMLLSLVLAGAIPASAWNIPAPQDYGKVIISNFSKKA
                     GMQPVRFDHWLHRSLYTCRLCHVDIGFAMEANATEITSEANRKGLYCGACHDGMKIYS
                     GKKIFAPCEQGERKAEDMTQCNRCHSVGIAGKTQEYDYATFTEKFPKRAFGKLI"
BASE COUNT          613 a          626 c          717 g          495 t
ORIGIN      
        1 gcgcaaggcc cgtatttccc ccgcgccgtg agtgtatgtc ctagtgacgt tctttaacag
       61 gataatgcgc cgcccgcctc agattctatt ttagttgcag cgaccggtct tgtaaaggtc
      121 cgctcggaga attatgacgg agcctgcaaa taatctgttt aaaaaaatcg gaattaatgc
      181 tatgatcctt tcatcaataa ggaggttttt atgaaggtca tcgcgttcaa cggcagcgcc
      241 cgcaaggaag ggaacacggc cgtcctgctg aacctggtgc tcgatgaact caaggccgaa
      301 ggcatcgaga cggagatgta ttcgctcgcc ggaaaaccga tccagggatg catcgcctgc
      361 atgaagtgtt tcgagaagaa gaaccggcgc tgcaacgtcg acaaggacat cgtcaacgag
      421 tgcatccaga agatgataga ggccgacggc atcctgctcg ggtctcccac gtattttgcc
      481 gacgtgtccg caagcatgaa ggcgctcatc gagcggtgcg gcatggtggg aaaggcgaat
      541 gccgacctgt acaagcgcaa gatcggggcg gccgtcgtgg cggtcaggcg cgcgggcggc
      601 atccatgtgt tcaattccct caattatttc tttacgatca gcgggatgat catcccgggg
      661 tcgagctact ggaacgtcgg cataggccgc acgccgggag aggtgaaggg cgatgcggaa
      721 ggggtgcaga ccatgaagga tctgggcagg aacatggcgt ggctgatgaa gaagctccat
      781 gcgtgacgtc gggcagaccc ggtgaggtgg ggaggagata ctccatgagg acgagaacgc
      841 tcttaatcgt gatcgtaatt cttgctgtgt ccggtttgac catggcgcag accggcgtca
      901 aaaagcggag gccgctcccg aatgaatacg gcagggtcgt tctgaataat tattcggaaa
      961 aagcgggtct cgcccctgtc gtgttcgatc attggctcca tcgggcaaaa ttcacgtgca
     1021 ggctctgcca cgtggatatt gcgtttgcca tgaaggcggg cacgacgggc attaccgcct
     1081 cggacaacat gaacggttta tactgcggct cctgccatag cggcaggatg gtgcaggatg
     1141 gcaagagggt ctttgaatcg tgctcaaaaa cgtatacgaa ggaagacatg acgaaatgcg
     1201 aacggtgcca ctcgctgggc aaaaacgtga agggggagta tgattttgcc aagtttacag
     1261 cgcgatttcc caaggagcgg ttcggtaacg ggataaactg ggaaaaggcc gaggaggacg
     1321 ggataattaa actcacggac tccatcgagg gcatatctat cccgagaaaa aaactcacga
     1381 ctccgaaaga cctggccctc ctgcccaaac aggttgagat gcccgaaata attttctccc
     1441 acaagaagca tgccgtatgg aacgggtgcg aactttgcca cccggagata ttcgggatca
     1501 aaaaagggac gactaagtat tccatgtccg acatttttga aggccggtat tgcggcgcct
     1561 gtcacgaccg cgtggccttt cccaatctcg attgccagcg atgtcataca agacccgttt
     1621 agaccttata aaggagcgcg ccttatggcc gtgaacaaag acctgtaccg aaccgccgtg
     1681 agtctggcca tcgccattgc agcagtcgtg tttctgccgt ccgggaactc gccgttcctt
     1741 tcgccggggt cggcgcatgc tcaggacgac tggaggaggg aattcgacag tatctgcgca
     1801 aagacccagg atgccgatac gttcagcccc caggagttga aggaactcat taaccgctgc
     1861 gatgaattga aaccccgcat cgagaagctt gaggagtcgc agcgcaaggt ggcgctgaaa
     1921 cggcttcaga tgtgcaggga cctgttcgtc ttcatgctgc agtccaagga gaacaagtga
     1981 agatgcggaa tatcgtccca tcgatgatgt tgctctctct tgttctcgcg ggcgcaattc
     2041 cggcatccgc atggaacatc cccgcacccc aggactacgg caaggtaatc atcagtaact
     2101 tttccaagaa agccggaatg cagccggtcc gctttgacca ctggctgcac cggtcgcttt
     2161 acacctgcag gctctgccat gtcgacatcg gcttcgccat ggaggcgaac gcaacagaga
     2221 tcacgtccga ggcgaacagg aaaggactgt actgcggtgc gtgccacgac ggcatgaaga
     2281 tctacagcgg caagaagata ttcgcaccct gcgagcaggg tgaacgaaaa gcggaagaca
     2341 tgacacagtg caatcgatgc cattccgttg gtattgcagg aaaaacgcag gaatatgact
     2401 acgccacttt cacggaaaaa tttcctaaaa gggccttcgg gaaactcatc g
//
LOCUS       DASSLD010000544         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_754442,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000544 DASSLD010000000
VERSION     DASSLD010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754442"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..978)
                     /locus_tag="VFK23_09835"
     CDS             complement(196..978)
                     /locus_tag="VFK23_09835"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET7319424.1"
                     /translation="MLVIADNLSIRNKAYMDAVKKKDKKAVAAMAKELAAKGAELINV
                     QVSSDGSGDEDALPFAAEAVQEGAGLPLCLDSRNVKALKKAVPLCKEPPIINYLSADE
                     KNPEDILSLVRETKSNLILRALKGSVPTTLEAKLMIIEDLVEKANAADIPNERLFADP
                     SVVHISKGMGQEHLLNAHESVVVLNEMVDPPVNTAVWLSNVTSGLPKDLKSTVASAYL
                     SYLAGAGLSAAMVNVNDAEIMKVVYLIKTFRDEITFAAGDLA"
     gene            1144..1998
                     /locus_tag="VFK23_09840"
     CDS             1144..1998
                     /locus_tag="VFK23_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HET7319425.1"
                     /translation="MLIEKLGLHHKDILVPRLKAAHVPLSEYSFANLYLFRRNHNYEV
                     IMDQEVFVKGISYDGRTYLMPTVDVRTVDPRYLKNLMADVNFLFPVPEAWLSAFEEPS
                     FEIAFIGGDADYLYTVEKMSTLGGRRLHKKRNLLKQFLETYEHDARPLTNDKLDEARF
                     VLEDWMKTSEMDPDATDYGPCREALDRYDELVLCGGIYYADGEPAGFVLGEELGTDTF
                     ILHFAKARTKFKGIYQYLFNTFAKILPPKYTYLNLEQDLDRENLRVFKGSYVPDSMLR
                     KARVGIKR"
BASE COUNT          409 a          602 c          571 g          438 t
ORIGIN      
        1 cagactccac gctgaccgtg gcgctggtgg tcaattactg aaaggtctag agtcagtgac
       61 cgttgggttt cctgcggcag gcgctgaatg agtctcatcc aatcaagaca catgaaaaag
      121 gcggaaccct gaagggttcc gcctttttct gttgcgcttg gtcggggcgg ggggtgatgc
      181 ctgaaaccaa aagggctatg ccaggtcccc ggcagcgaag gtgatctcgt cccggaacgt
      241 cttgatcaag tagaccacct tcatgatctc tgcgtcgttc acgttcacca tggctgcgct
      301 cagtcccgcg cccgcgaggt aggagagata ggccgaggcc acggtggact tcaggtcctt
      361 cggcaggccg gaggtcacat tcgaaagcca gaccgccgtg ttgaccggcg ggtcgaccat
      421 ttcgtttaag accacaacgc tctcgtgggc gttcaggagg tgctcctggc ccatgccctt
      481 gctgatgtgc accaccgacg gatcggcgaa cagccgctcg ttcgggatat cggccgcatt
      541 cgccttttcg acgaggtctt cgatgatcat gagcttggcc tcgagcgtgg tgggcacgct
      601 ccccttgagg gcccggagga tgaggttcga cttggtctcg cgcacgagcg acaggatgtc
      661 ctcgggattt ttttcatcag cggaaaggta gttgatgatc ggcggctcct tgcagagcgg
      721 gacggccttc ttgagcgcct tcacgttcct cgaatccagg cagagcggca ggcccgcccc
      781 ctcctgcacg gcctcggccg cgaaggggag ggcgtcctcg tcgccgctgc cgtcggacga
      841 gacctgcaca ttgatcagtt ccgcaccttt tgccgcgagt tcctttgcca ttgccgcgac
      901 ggccttcttg tccttcttct ttaccgcatc catgtaagcc ttgttcctga tgctgagatt
      961 gtccgcgatt acgagcatgc catcctcctt gaatattttt cccagccagc catcattccg
     1021 gaatagcttc ggccggaagc cagtgccgtt attttcccaa gtataccata attcccctcc
     1081 catccggcag gaagaccccg gggtcaatct tgacaaaatc ccgatatcct ctattattga
     1141 ggcatgctga tcgagaaact gggcctgcac cataaggaca ttctcgtccc ccggcttaag
     1201 gccgcccatg tccccctttc cgagtattct tttgccaatc tgtacctttt ccgcaggaac
     1261 cacaattacg aagtaatcat ggaccaggag gtcttcgtta agggtatttc atatgacggc
     1321 cgcacctacc tgatgccgac cgtcgacgtg cgcaccgtgg acccgcggta tctcaagaac
     1381 ctcatggcgg acgtcaattt ccttttcccg gtccccgagg cgtggctttc ggcttttgaa
     1441 gagccttctt tcgagatcgc cttcatcgga ggagacgctg actacctgta caccgtggag
     1501 aagatgagca ccctgggcgg cagaaggctc cacaagaaga ggaacctctt gaagcagttt
     1561 ctcgaaacgt acgagcacga cgcccggccc ctcacgaacg acaaactcga tgaggctcgc
     1621 ttcgtcctgg aggactggat gaagacgtcg gagatggacc ccgacgccac ggattacggg
     1681 ccctgccgcg aggcgcttga ccggtacgac gagctcgtcc tctgcggagg catatactat
     1741 gccgacggag agcccgcggg tttcgtgctc ggggaagaac tcggcacaga caccttcatc
     1801 ctgcacttcg ccaaggcgcg gacgaagttc aaggggattt accagtacct gttcaacacc
     1861 tttgccaaaa tcctgccgcc gaaatatacc tacctgaacc tggagcagga cctggacagg
     1921 gaaaacctgc gcgtgttcaa agggtcctac gtgcctgatt cgatgctgag gaaggcgcgg
     1981 gtgggcatca agcgatgaca atcccccgga aaaaagtcgg
//
LOCUS       DASSLD010000545         3527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_76080,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000545 DASSLD010000000
VERSION     DASSLD010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76080"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFK23_09845"
     CDS             <1..373
                     /locus_tag="VFK23_09845"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HET7319426.1"
                     /translation="KLSCPEGSETMKIDSYTFGAIVINGKTYTSDVIIYPGRVESTWW
                     RKEGHLLQFADVEKALQARPEVLVIGTGYSGVMRVPAETVDRIAGQGIEVRVEQTAKA
                     VDVYNRLQGVKTVIAALHITC"
     gene            387..1322
                     /locus_tag="VFK23_09850"
     CDS             387..1322
                     /locus_tag="VFK23_09850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET7319427.1"
                     /translation="MKTLVYHDGALGDMLLSLPCLRRLKAQSHEVHLAGRGDVVRFLR
                     DTGVVDAASSSDGALFASLYSAVDLRLRTFLSGFNRALVFSTEEHPASALAIGTVIPD
                     TRTIRTIPPGGSQTHAAQYRLSQLEPDAPVSSHYTVLSVPPKEAKAAQSYLVEAGYRS
                     GAGLVAVHPGSGGRPKRWPLERFFGLIERLQSGSDAFVVVFTGEAEDGEIRQAVCRRA
                     RGRKNMLHADGLPLLSAAALLSHCGLYVGNDSGFSHLAGALGCPTIVLFGPTNPLVWK
                     PLGPRVETVSTGVPGPMARISADEVIAKIRSMVPR"
     gene            1557..1943
                     /locus_tag="VFK23_09855"
     CDS             1557..1943
                     /locus_tag="VFK23_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019028297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7319428.1"
                     /translation="MKLLEKYEDHAYALMRIAAGFMFSFHGAQKILGVLSQFQPPIGS
                     QLWFGGLIELLCGLAIMLGYRTRLAAFLASGEMAVAYFQFHWKFQLGAQFFPTVNKGE
                     LAALYCFVFLFIACRGASKWGLDKTG"
     gene            2020..2997
                     /locus_tag="VFK23_09860"
     CDS             2020..2997
                     /locus_tag="VFK23_09860"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET7319429.1"
                     /translation="MSTAIKNKEELTIARIFDAPRELMWKAWTDPEHLKKWWGPKYFT
                     APYSMIDLRVGGRYLHAMRGPDGKDYWSTGIYREIVPLERFVCTDSFADDKGNVVPAS
                     HYGMAGDWPLELRVKVTFEDLGGRTKMTLRHEGIPAGPMLEQCEIGWSESFDKLAEYI
                     VPVDRTRITAERGKQEVIITRVYDAPRNLVFKACTDRNLIAQWWGPKRFTTAIDKMDV
                     RPGGVWRYVQRDAAGNTYAFHGVYHEVRSPECIIDTFEYEGAPGHVSLETLALEDLGG
                     KTQWTARSVFQTVEDRDAMLKSGMEEGVYETMDRLAELLGKLKIERKAA"
     gene            3255..>3527
                     /locus_tag="VFK23_09865"
     CDS             3255..>3527
                     /locus_tag="VFK23_09865"
                     /inference="COORDINATES: protein motif:HMM:NF037053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET7319430.1"
                     /translation="MLIVLHHSASEKDVENIKKTITKMGLKPVAIPGAERTAIGVIGN
                     QGWVDDAPLKGNKAIMEIIHVTKPYKLVSRDFHPRDSVVTLGGKVKV"
BASE COUNT          752 a          963 c         1090 g          722 t
ORIGIN      
        1 aaaattatca tgtccggaag ggagtgagac catgaagata gattcctata ccttcggcgc
       61 catcgtcatc aacgggaaga cctatacctc cgacgtcatc atttatcccg gcagggtcga
      121 gtccacgtgg tggcgaaagg aggggcacct gctccagttc gcggacgtgg aaaaagcgct
      181 gcaggccagg ccggaggtgc tcgtcatcgg gacgggatat tcaggcgtca tgcgcgttcc
      241 ggcggaaacc gtcgaccgga ttgcgggcca ggggatcgag gtgagggtgg agcagacggc
      301 aaaggcggtc gatgtttata accggctcca gggcgtgaag acggtgattg cggccctgca
      361 tatcacgtgc tgattgacca taggtaatga agacgcttgt ttatcacgac ggcgccctgg
      421 gcgatatgct cctctctctt ccctgcctca ggaggctcaa ggcacagtcg catgaagttc
      481 acctggccgg gagaggggac gttgtccgct tcctgcggga cacgggcgtc gtggacgctg
      541 cctcgtcgtc cgacggggcg ctctttgcgt ccctctactc ggcagtcgat ctccggctgc
      601 ggacgttcct gtccggattt aaccgggccc tcgtgttctc gacggaggaa catcccgcct
      661 cagcactagc gatcgggacc gtcatccccg atacccgtac gatcaggacc atccctcccg
      721 gcggatcgca gacgcacgcg gcgcaatacc ggctctcgca gctcgaaccg gacgccccgg
      781 tctcctctca ttacacggtt ctgagtgtgc ccccgaagga ggcgaaggca gcgcagtcgt
      841 atctcgtcga agcgggctat agatccggcg cgggcctcgt cgccgtgcat ccgggaagcg
      901 gcggcaggcc gaaacgctgg cccctggagc ggtttttcgg gctcatcgaa cgcctgcaat
      961 cagggagcga tgcgttcgtg gtcgtgttca cgggtgaggc cgaggacggg gaaatccggc
     1021 aagcggtctg ccgccgtgcg cgcggccgga agaacatgct tcatgccgac ggtcttcctc
     1081 ttctgtcagc ggccgccctc ttatcgcact gcgggctgta cgtcggaaac gactcggggt
     1141 tttcccatct cgcgggcgcg ctgggctgcc ccacgatagt tcttttcggg cctacgaatc
     1201 cgctcgtctg gaaacccctg ggccctcgcg tggaaacggt ttcaacgggc gtccccggtc
     1261 cgatggctcg gatttcggca gacgaggtga tcgcgaagat aagatcgatg gttccgcgat
     1321 aagtaaaaat ttaccactga ggcgccgaga cactgagaaa agctttaata aagtcgaatg
     1381 gtttcctccg cgttctctgt gactctgcgg ttagatatga tttttcacgg attcatcgtg
     1441 attgtctttt ttagcaggtc acaatatgcc cgaagaaaac ttaccatact tttgtctctg
     1501 atgtataatt aatcaagacg tgttttgtcc tgatccgggg aaaggagcct ctatccatga
     1561 aactgcttga aaagtatgag gaccacgcgt acgccctgat gcgcatcgcg gcgggattca
     1621 tgttctcgtt ccacggggcc caaaagatcc tgggcgtgct ctcccagttc cagccgccca
     1681 tcgggtccca gctctggttc ggcgggctca tcgagctcct gtgcggactg gcgatcatgc
     1741 tgggatatcg gacccgcctg gcggctttcc tcgcgagcgg cgaaatggcc gtggcttatt
     1801 tccagttcca ttggaagttc cagctcggag cccagttctt cccgaccgtg aacaagggcg
     1861 agttggcagc tctttactgc ttcgtgttcc tcttcattgc ctgccgggga gcgtccaaat
     1921 ggggcctgga caagacggga tgagaggaaa ctctcggagg gggtcacgga acccgagtcg
     1981 gagacatccg tgatgatgtg gctgaaaagg aggtatcaca tgtcaactgc aataaaaaat
     2041 aaagaggagt taaccatcgc gcgcattttc gacgcaccgc gcgagcttat gtggaaagcg
     2101 tggaccgatc ccgagcattt gaagaagtgg tgggggccga agtatttcac cgcgccctac
     2161 agcatgatcg acctccgcgt aggaggcagg tatctccatg ccatgcgcgg accagacggc
     2221 aaggactact ggtcgacagg catttaccgt gagatcgttc cgctggagcg attcgtctgc
     2281 acggacagct ttgccgatga taaaggcaat gtggtcccgg cctcacacta cggtatggcc
     2341 ggggactggc cgcttgaatt acgggtgaag gtgacgttcg aagatctcgg cggcaggaca
     2401 aaaatgaccc tccggcatga agggattccg gcggggccta tgctcgaaca gtgcgagatc
     2461 ggctggagcg agtctttcga caagctcgct gaatacatcg tgcccgttga ccggaccagg
     2521 atcaccgccg aacggggaaa acaggaggtc atcatcacgc gggtctatga cgcgcctcga
     2581 aacctcgtgt tcaaggcatg cacggaccgg aatcttattg cgcaatggtg ggggccgaag
     2641 cggtttacga ccgcgattga caagatggat gtgagaccgg gtggtgtatg gcgctatgtc
     2701 cagcgcgatg ccgcgggcaa cacgtacgct ttccacggcg tgtaccacga ggtccgttcg
     2761 ccggaatgca tcatcgatac ctttgaatac gagggcgcgc cgggccacgt atcgctcgaa
     2821 acgctggccc tggaagacct cggcggcaag acacagtgga cggccaggtc cgtattccag
     2881 acagtcgagg accgcgacgc catgctcaag tcgggcatgg aggaaggggt gtacgagacc
     2941 atggaccggc ttgccgagct tctgggcaaa ctgaagattg aaagaaaggc cgcatgagaa
     3001 gaggggccga atcggttagg gatgacgcac gatgtcgtca aggaggactc gggatgtgat
     3061 cgaggtcctc cttctttttt ccgcgatccg agaaatgatt tgacagcgcg ttgcgattaa
     3121 tcttatagtg aatattagtg gcgaggcctt tggccctatt caaaaagctg ttacgggttg
     3181 ccggttacga gttacgggtt taactcgtaa ctcgcatcac gcaacacgca acgagaattc
     3241 agcaaggagt ttgcatgctt atcgtactgc accattcggc aagcgaaaag gacgttgaaa
     3301 acatcaagaa gaccatcacg aagatgggtt taaaaccggt cgccatcccg ggcgccgaac
     3361 ggacggccat cggcgtgatc ggcaatcagg ggtgggtgga cgacgcgccc ctcaagggca
     3421 acaaggcgat catggagatc atccatgtca cgaagcccta caagctcgtg agccgtgatt
     3481 tccacccgag ggactcggtt gtaacgctcg gcggcaaagt caaggtc
//
LOCUS       DASSLD010000546         2504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1062062, whole genome shotgun sequence.
ACCESSION   DASSLD010000546 DASSLD010000000
VERSION     DASSLD010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062062"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..443
                     /locus_tag="VFK23_09870"
     CDS             285..443
                     /locus_tag="VFK23_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319431.1"
                     /translation="MKETETTGRTEEQDKEIVNILIDSDLYLDMELAERKNLLHFIET
                     AYFSCPAK"
     gene            complement(547..1374)
                     /locus_tag="VFK23_09875"
     CDS             complement(547..1374)
                     /locus_tag="VFK23_09875"
                     /inference="COORDINATES: protein motif:HMM:NF025711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HET7319432.1"
                     /translation="MGPMLDKVSDPTSSQSRGSFECGNCGRQFDATVVTWVDVARTPQ
                     ARQALLDWQFNLIQCTYCGCRHFSGTPFFYEDLSEGLLVAVFPAIPDKRGELEKSVRR
                     QYSYYPVIEFFYDMTQIWMLLYFQKQYRMNRNLQRLSRIGEGEKRLRVMLRFVKENRL
                     MIAIREKLTEAFFEEEDDDELAEILRKAIYLLEEMLPWPEDKRCICGAHLAGELSCCG
                     KTIDLEEHHQLLSQDYVVYCSLCKEALSGASCKECGRVYTWKLGTVASYGREGGADC"
     gene            1470..>2504
                     /locus_tag="VFK23_09880"
     CDS             1470..>2504
                     /locus_tag="VFK23_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET7319433.1"
                     /translation="MPTQDTSILTTALHSEVESRFLTYALSTIVSRSLPDVRDGLKPV
                     HRRILFAMWEMGLGPTAKFRKSAAVVGEVLGKFHPHGDQAAYDAMVRMAQPFSLRYPL
                     VEGSGNFGSVDGDPAAAMRYTEARLSRLAEELLIEIEKETVGFRPNYDNSLKEPVVLP
                     ARFPQLLVNGTSGIAVGMSCSFPPHNIGEVIDGLAAMIDNSNIEAKDLLKHIKGPDFP
                     TGGQILNSKKELREIYETGSGGIRVRGEYDVEDLPRGRQAIVFTSIPYLVNKAKLLEK
                     LIGLMEDKKLPQVSVVRDESTHEVRIVLELKAETNVELVTAYLFKHTDLESTFPVNFT
                     ALKPNMEPDRL"
BASE COUNT          577 a          733 c          673 g          521 t
ORIGIN      
        1 ctgcgcccac aaagagtcaa cggtttttct ccggcagggt aaaccgaaga tccctcctcg
       61 cggccagctt atagtgcagc agccggatag gaaatccaac cttctactgc agggttgccg
      121 tcaaagtagg tcaaaatgac ctacctgatg ggtcatttta ccatctcaat agaccagaag
      181 gctgtcctca attcattaat atattgatat ttataggttt gaagttatgg cacacaatat
      241 gcttgatatt taattagaaa gcaaataagg ttgggtgaaa caaaatgaaa gagacagaaa
      301 caactggtcg gaccgaggag caggacaaag agatcgtcaa tattttgatc gacagcgacc
      361 tttatctgga catggaactg gcagagagaa agaatttact gcatttcatc gagactgcct
      421 atttcagctg ccccgcgaag tagataacaa accatttccg aggccccttc cccggataat
      481 gccctcctgc cctgctcttc tacgaaaagc cttcacgcaa gcgcgggatt ccggaccatc
      541 ggaatttcag cagtctgcgc ctccttcccg tccgtacgag gctacggtgc cgagcttcca
      601 agtatagacc cttccgcatt ctttgcagct ggcgcctgag agggcctcct tgcacaggga
      661 acaatagacg acatagtcct gggacagaag ctgatgatgt tcctccaggt cgattgtttt
      721 accgcagcac gaaagctctc cggcaagatg ggccccgcag atgcaccgct tgtcttcagg
      781 ccacggaagc atctcttcca gcagataaat cgctttccga aggatttccg cgagctcgtc
      841 gtcgtcctcc tcctcgaaga aggcctcggt cagcttctcg cggatggcaa tcatgagacg
      901 gttttccttc acaaaccgga gcatgacgcg gagacgcttc tcgccttctc cgatcctcga
      961 caaccgctgc aggttcctgt tcatccggta ctgcttctgg aaatacagga gcatccagat
     1021 ctgcgtcatg tcgtaaaaga attcgataac gggatagtaa ctgtattgtc tgcggaccga
     1081 tttttccagt tctccccgct tgtcgggaat ggcggggaac acggccacga gcagcccttc
     1141 ggagaggtcc tcgtagaaaa aaggcgttcc ggaaaagtga cggcacccgc aataggtgca
     1201 ctggatgaga ttgaactgcc agtcgagcag ggcctgcctt gcctgcggcg tcctggctac
     1261 atcgacccac gtgacgaccg tggcgtcgaa ctgccgcccg cagttgccgc attcaaacga
     1321 cccccggctc tgcgacgatg tcggatcgct caccttgtcc agcatgggcc ccattatacc
     1381 cccttttcgc gaaacttgtt ttgaaaatta ttctgagatt gtggtacaat gcggacccga
     1441 taaggacatc gagacaaagg gggcaactga tgcctacaca ggatacttcg attttaacca
     1501 cggcgctgca ctcggaagtc gagagccggt tcctcaccta cgcactgtcg accattgttt
     1561 ctcggtcact ccccgatgtg cgggacggct tgaaaccggt gcaccgcagg atcctcttcg
     1621 cgatgtggga aatgggcctg ggccccacgg cgaagttccg caagagcgca gccgtggtgg
     1681 gcgaggtgct gggaaagttc catccccacg gggaccaggc ggcctatgac gcaatggtga
     1741 ggatggcgca gcccttctcg ctccggtatc cgctcgtcga gggctcggga aacttcggca
     1801 gcgtggacgg cgaccccgcc gcggccatgc gctataccga agcgcgtctc tcgcgtctcg
     1861 ccgaggagct tttgatcgag atcgagaaag aaaccgtcgg cttccggccg aactacgaca
     1921 actcgctcaa ggagccggtc gtcctcccgg cccggttccc ccagctcctc gtgaacggca
     1981 cctcggggat cgccgtgggc atgagctgtt ccttcccgcc ccacaacatc ggcgaggtga
     2041 tcgacggcct cgccgcgatg atcgacaatt cgaacatcga ggcgaaggac ctccttaaac
     2101 acatcaaggg cccggacttt ccgaccgggg gccagatact gaactcgaaa aaggagcttc
     2161 gcgagattta cgaaaccggt tccggcggca tcagggtccg cggagaatac gacgtcgaag
     2221 atcttccccg gggcagacag gccatcgtgt ttacctccat cccctatctc gtgaacaagg
     2281 ccaagttgct cgaaaagctc attggcctga tggaggataa gaagctgccg caggtgtccg
     2341 tcgtccgcga cgaatccacc cacgaagtgc gtatcgtgct cgagctcaag gcggaaacga
     2401 acgtggagtt ggtgaccgcc tacctcttca aacacacgga ccttgagtcc acgttcccgg
     2461 tgaacttcac ggccctgaaa ccgaacatgg agcccgaccg cctg
//
LOCUS       DASSLD010000547         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1218262, whole genome shotgun sequence.
ACCESSION   DASSLD010000547 DASSLD010000000
VERSION     DASSLD010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218262"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..466
                     /locus_tag="VFK23_09885"
     CDS             137..466
                     /locus_tag="VFK23_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7319434.1"
                     /translation="MKNPANAIASRQTSKGVEGYVYDGVDGSQATIFQCAKDGISKEH
                     VHDFDEYFVVIQGEYILGLEGKRLKLRAGDEYYIPRGTPHDGAFTAGTRTFHAFGGKR
                     ADRKGNR"
     gene            514..957
                     /locus_tag="VFK23_09890"
     CDS             514..957
                     /locus_tag="VFK23_09890"
                     /inference="COORDINATES: protein motif:HMM:NF020400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HET7319435.1"
                     /translation="MARIGILTCSNATQDLGCSSVSCLADFRRRKGAFADYPSGEKLT
                     LTGIINCPGCPTLTGPDKLVARIRALTDFRVDAVHLSYCVKALCPFREKYRKALEQSF
                     PNIRIVVGTHEDHVTPEEFRRRVKKLFCQPQKNMVDIILNKDEER"
     gene            1028..1480
                     /locus_tag="VFK23_09895"
     CDS             1028..1480
                     /locus_tag="VFK23_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319436.1"
                     /translation="MMNIPIDKNRSWMSRIYESIDRLDQGLQAAIMKPAGQACADDIV
                     MLCEKYLGKKISTIDELVAGWNMIRDKRNLTGRWESHGNTIRGVFGECGCPLVRSGLI
                     ELHPVQCYCSRGMMDAIFSRVAKRAVEIRINRSIGRGDNCCEFVVTIP"
     gene            1582..2037
                     /locus_tag="VFK23_09900"
     CDS             1582..2037
                     /locus_tag="VFK23_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7319437.1"
                     /translation="MFNDTNEHEYRISTDKKRLDLAMIHDFLANESYWAKGIPLEVMK
                     RSIKNSLCFGVYHQGRQVGFARVVTDYATTAYVGDVFIRESYRGRGLGKRLIKAVVDH
                     AALEGLRMWFLGTKDAHGLYRKFGFKNAADTPVMERLMVILNKDVYIKT"
     gene            2092..>2497
                     /locus_tag="VFK23_09905"
     CDS             2092..>2497
                     /locus_tag="VFK23_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7319438.1"
                     /translation="MEPGIRSKTLITIIVFVCALAANSYSASDGFKTIDTTQLHVMIV
                     DNAYSLEGGRGLKFIIVDVRTKEEFDKSHIASAINIPERELEKFSALLPKDKGTLIVL
                     YGNDKALVTSRKWADKAKAAGYTDIVIYSETLE"
BASE COUNT          678 a          586 c          697 g          536 t
ORIGIN      
        1 aatacgaatt actgaaaaag aaactctatt atttgtcgta caactgacgg aatagaaagc
       61 atgtctgtgc gaacgaggag gttgccggca gacattggaa aggagagaaa aaaacgtgaa
      121 tgcgtttccg aaatttatga aaaatcctgc caatgcaatc gcgtctcgcc aaacgagcaa
      181 gggcgttgaa ggatacgtgt atgacggcgt ggatggaagc caggcgacga tatttcagtg
      241 cgcgaaggac gggatatcaa aggagcatgt ccatgatttc gatgaatact tcgtcgtgat
      301 ccagggagag tatattctgg ggcttgaagg aaagcggctt aaactcaggg ccggcgacga
      361 atattatatt ccccggggaa cgccgcatga cggggccttc actgccggca cgagaacgtt
      421 ccatgcgttc ggcgggaagc gtgcggaccg aaagggtaac cgatagcggg accatgagca
      481 agagctgtca accaaaacag gggagtgttc gatatggcga gaataggcat tttgacgtgt
      541 tccaacgcaa cgcaagacct cggttgttcg tctgtaagtt gtcttgctga ttttcgaagg
      601 aggaaagggg cctttgcaga ttatccctcc ggagaaaaac tgacgttgac gggcatcata
      661 aactgtcccg gatgtcccac gctcaccggc cccgataaac tggtggcgcg aatccgggcg
      721 ctcaccgatt tccgggtcga tgccgttcat ctgtcctact gtgtgaaggc gctttgcccg
      781 ttcagggaaa agtaccggaa agcgctcgaa caatcctttc cgaatatcag aatcgtggtc
      841 ggcacgcacg aagaccatgt caccccagaa gaatttcgcc gccgcgtcaa gaaactgttc
      901 tgccagccgc agaaaaacat ggttgatatc attctgaaca aagacgagga gcggtaagga
      961 catctctcgc gatccttctg gcgttcttct ttccggtgga gaagcgaacc aaatttatac
     1021 gaggtgtatg atgaacatcc ccattgataa aaacagaagc tggatgtcgc gcatctacga
     1081 aagtatcgac cggctcgacc aaggcttgca ggctgctatc atgaagccgg cgggacaggc
     1141 atgtgcggat gacatagtca tgctttgcga aaaatatctc ggcaaaaaga taagcaccat
     1201 tgatgaactc gtagccggct ggaatatgat tcgcgacaag cgcaacctca cgggcagatg
     1261 ggaatcgcac ggaaatacta tccggggagt attcggcgaa tgcggatgcc cgcttgtccg
     1321 gtcaggattg atcgagctcc atccggtgca atgctattgc tctcggggca tgatggatgc
     1381 gatattttcc cgggttgcga aaagggccgt tgaaatcaga atcaaccgct cgatcggccg
     1441 gggagataat tgctgcgaat tcgtggtaac gatcccatag acccttggcg cgggccaagg
     1501 atgttatatc ctcaaaggca cgccgcattc ctgaacgttc tatagcggac acgcgcacga
     1561 tacattgttc cggagaaaaa cgtgtttaac gatacgaatg agcatgaata cagaataagc
     1621 acggataaga agcggctcga tctcgccatg attcacgatt ttcttgcaaa cgagtcctac
     1681 tgggccaagg ggatccccct ggaggtgatg aagaggtcca tcaaaaactc gctctgcttc
     1741 ggcgtgtatc atcagggcag gcaggttggc tttgccaggg tcgtgacgga ttacgccacc
     1801 accgcctatg tgggcgatgt cttcatcaga gaatcctacc ggggaagggg gctcggaaaa
     1861 cgactgatca aagccgtcgt ggaccatgcc gcgctcgagg gcctgcgaat gtggttcctc
     1921 gggacgaagg acgctcacgg cctgtaccgg aagttcggtt tcaagaacgc agcggatacg
     1981 ccggtcatgg aaagattgat ggtgatcctg aataaggatg tatatattaa gacatagtgt
     2041 ctcactgctc gagcagggaa tttgagggat gccaatgaaa ggaggacatc gatggaacca
     2101 ggcatacgat caaaaacact catcaccatt atcgtttttg tatgtgcgct cgccgcaaat
     2161 tcgtactccg cttccgatgg tttcaaaact atcgacacga cacagctcca tgttatgatc
     2221 gtggataatg cgtacagtct tgaaggcgga agagggctca agtttatcat cgtcgatgtc
     2281 cggacaaaag aagaattcga caagtctcac attgccagcg caattaacat ccctgaacgg
     2341 gagcttgaga aattctcggc gcttcttccg aaagacaagg gaacattgat tgtcctgtac
     2401 ggcaatgaca aagcgctcgt tacaagccgg aaatgggcag acaaggccaa ggccgcggga
     2461 tataccgaca tcgtcattta ttcggaaacg ctggaaa
//
LOCUS       DASSLD010000548         3490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1327252, whole genome shotgun sequence.
ACCESSION   DASSLD010000548 DASSLD010000000
VERSION     DASSLD010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327252"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..299)
                     /locus_tag="VFK23_09910"
     CDS             complement(78..299)
                     /locus_tag="VFK23_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319439.1"
                     /translation="MSEPLVSKGKKEFYLCGASGRRLVTRLDKDGTPDNEPFGSMVRG
                     DVVRFEGLLDEGKRYKVGKDTRVRVRNIR"
     gene            complement(313..780)
                     /locus_tag="VFK23_09915"
     CDS             complement(313..780)
                     /locus_tag="VFK23_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HET7319440.1"
                     /translation="MSRYRHPQKAGSKYITPEGKKRLSEEFEDLWRTKRPEVTAALAA
                     AAAEGDRSENAEYIYRKKQLREIDARIHHLKERLEHIVVVDSRPDNRSQVFFGAWVRL
                     EDEEGNRSEYRIVGPAYRNEGTGQAHGAQERFAQVFLARLSEGPAVGSRILWR"
     gene            complement(826..1923)
                     /locus_tag="VFK23_09920"
     CDS             complement(826..1923)
                     /locus_tag="VFK23_09920"
                     /inference="COORDINATES: protein motif:HMM:NF020804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HET7319441.1"
                     /translation="MHKDDLKALSEKVARLSLLLTRDREDLPAAYLKDAGLREAYLSY
                     FLPPNLRKIHVPVGELARHPEGLLEKDRLRVLDLGSGPGTALLGVLEFFAEQEHRPHL
                     EFVALDLVAENLKITEDLFASAQSGKALPASLKTIRSSIESAGQHVTGTFDLIIFSNV
                     LNELFIREGRKIEKQCVVLEDIMTRLLADDGSLIVIEPALRETSRGMLEVRDRLLAAG
                     YRLYAPCFFRGNCPALANPRDWCHEDIPWDPPAVIRELDRLTGLRKDSLKFSWLVFRK
                     DRRSAAGFYGDDAFRVVSEPLVSKGKREFYLCGAAGRRLVTRLDKDGTPDNEAFGSMV
                     RGDVVRFEGLLDEGKRYKVGKATRVRVRSIR"
     gene            complement(2079..2756)
                     /locus_tag="VFK23_09925"
     CDS             complement(2079..2756)
                     /locus_tag="VFK23_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319442.1"
                     /translation="MATTTQETYHEETKDWYWKGGSLAEGIAGGAGVALAILGLANIM
                     QNYMIPVSTIALGIAFVYEGGAVARRFAWLLGEATKGRYDVSGLGSGLTAEVIGGLAG
                     IILGILALLNVSPTVLMPAAAIVYGGTLVYSSGISARLDEMWIWRTSEYEAYKDVAHE
                     AVWASAGMSLLFGISAVTLGILALVGLSPMYLNLVALLGVGLSNLLSGAAISSRLLNT
                     IRMHQHA"
     gene            3141..>3490
                     /locus_tag="VFK23_09930"
     CDS             3141..>3490
                     /locus_tag="VFK23_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7319443.1"
                     /translation="MNNTIILVAAMAGLSLLAGYTTADALFWQNEVTGAALPVMLPRE
                     RYFLSFCIIGIFLCGVIIFRVLSEQKYAEQSQRETEVTLSAVTDAANEAIVMVNAGST
                     VTFWNPAAEKIFGY"
BASE COUNT          761 a         1018 c          924 g          787 t
ORIGIN      
        1 cccgctttct ggggatggcg atacctgctc atatcgggga gataatacgg taaacagaag
       61 cgggaatcaa gatggaatca gcggatgttc ctcacgcgta ctctggtatc cttgccgact
      121 ttatatcgct ttccctcgtc gagaagccct tcgaagcgga cgacatcgcc ccgcaccatc
      181 gagccgaagg gttcattgtc cggagtcccg tctttgtcca accttgttac gagccttctc
      241 ccgctcgcgc cgcagaggta aaactccttc ttcccctttg aaacgagcgg ctcactcaca
      301 acacggaagg cgtcatcgcc atagaatccg gctgccgacc gccggtcctt ccgaaagacg
      361 agccaggaaa acttgagcga atctttcctg agccccgtga gcctgtccag ttccctcatt
      421 acggtaggcg ggtcccacga tccgatactc actcctgttc ccctcctcgt cttcgagccg
      481 cacccaggcg ccgaaaaaca cctgggacct gttgtccggc cggctgtcca cgaccacgat
      541 gtgttcgaga cgctccttga gatgatggat ccgggcgtcg atctcgcgaa gctgcttctt
      601 gcggtagata tactcagcgt tttccgaccg gtcgccctcc gccgcggcag ccgcaagcgc
      661 ggccgtcacc tcggggcgct tggtccgcca cagatcttcg aactcttcgg agaggcgctt
      721 cttgccttcc ggggttatgt atttggaccc cgctttctgg ggatggcgat acctgctcat
      781 atcggggaga taatacggta aacagaagcg ggaatcaaga tggaatcagc ggatgctcct
      841 cacgcgtact ctagtagcct tgccgacttt atatcgcttg ccctcgtcga gaagcccttc
      901 gaagcggacg acatcccccc gcaccatcga gccgaaggct tcattgtccg gagtcccgtc
      961 tttgtccaac cttgttacga gtcttctccc tgccgcaccg cagaggtaga actccctctt
     1021 cccctttgaa acgagcggct cgctcacaac acggaaggcg tcatcgccgt agaatccggc
     1081 tgccgaccgt cggtccttcc gaaacacgag ccaggaaaac ttgagcgaat ctttcctgag
     1141 ccccgtgagc ctgtccagtt ccctgattac ggcaggcggg tcccagggaa tgtcttcgtg
     1201 acaccagtcc ctggggttcg cgagggccgg gcaattcccc cggaagaaac agggggcata
     1261 gaggcgatac cccgcggcaa gcaaccggtc cctgacctca agcatcccgc gcgacgtctc
     1321 ccgtagggcg ggttcgatga cgatgaggct gccgtcgtcc gcgaggagac gggtcatgat
     1381 atcttccagc acgacacatt gtttttcaat ctttcgccct tcacggatga agagctcgtt
     1441 gagaacgttc gagaagatga tgagatcgaa ggtcccggta acgtgctgcc ccgcgctttc
     1501 gatgctggat cggatggtct tcagggaagc cgggagcgcc ttcccactct gcgccgaggc
     1561 gaataaatcc tcggtgattt tcaagttctc agccacgagg tcgagggcga cgaactcgag
     1621 atggggacga tgctcctgct ccgcgaagaa ttcaagcacg ccgagcagcg cggtgcccgg
     1681 cccggagccc aggtcgagga cccgaagcct gtctttttcc agcagccctt cgggatggcg
     1741 ggccagctcc ccgaccggca catgaatttt ccggaggttc ggagggagaa aatacgagag
     1801 atacgcctct cgcagtcccg cgtccttgag atatgcggcg ggcaggtcct cccggtcacg
     1861 ggtaagcaga agagagagcc gtgcgacctt ttccgaaagg gccttcaaat cgtctttatg
     1921 cattcgggcg cgagtatcca tcgatggtcg tgatgttata ccatggcaac ggggggaaaa
     1981 caactgcgcg atcgtgattt ccgtggacaa aaaaagccgg ggatcattat ctgggacccc
     2041 cggcttcaag acgttcttgc ttcctcgcgt ttcctccgtt atgcgtgctg atgcattctg
     2101 atcgtgttca gcagacggct gctgatcgcc gctccgctca gaagattcga gaggccgact
     2161 cccagcagtg ccacgaggtt caggtacatc gggcttaagc ccaccagggc gagaatgccc
     2221 agggtgaccg cgctgattcc gaagagcagc gacataccgg ccgatgccca tactgcttca
     2281 tgcgcaacgt ccttgtatgc ctcgtactcg ctcgtccgcc agatccacat ttcgtccagc
     2341 cgcgcggaaa ttccgctgct gtagaccagc gtaccgccgt acacgatggc tgcggccggc
     2401 atcaggaccg tgggggaaac gttcagaagc gccaggatcc cgagaataat tccggcaagg
     2461 ccgcctatga cttcggcagt cagtccggaa cccaggcctg acacgtcata ccgccctttt
     2521 gtcgcctcgc ccagaagcca tgcgaacctt ctcgcgacgg ctcctccttc atagacgaac
     2581 gcgataccga gggcaattgt tgataccgga atcatgtaat tctgcatgat gttcgccaga
     2641 ccgaggatgg ccagcgcaac gccagcgccg ccggcaatcc cttcggcgag cgatccgcct
     2701 ttccagtacc aatcttttgt ctcctcatga taagtttcct gggttgtggt tgccatatat
     2761 aacacctcct tacccagtgc agcggataat aaataggctg cacttaactt accatatgat
     2821 attataactc tttgtattta tgtgtcaatc agacaataat agcaggaaag tgcagtaaat
     2881 atcgcaacga agagtcatga tactaagatt gcacaagcaa gcaatcattc tcgatcgcaa
     2941 agcatgacat tttttgcttt tttgtgtctt ttttgccagt atgcccgtgt cattcattgc
     3001 acagataagg aatttgtaac atatttttcc ttcagtagtt cttgtaacgt cttgaattaa
     3061 aaacaactaa tattgggcat accggttgca gctttctaga tataaagcca ttaaaattcc
     3121 tgaaaattat gaagaaaaat gtgaataaca ccataatcct cgtggcggcc atggccggtc
     3181 tgtctctgct tgccgggtat acgaccgcag acgccctgtt ttggcaaaat gaagtaaccg
     3241 gggccgcgtt gcctgtcatg ttaccccggg aacgctattt tctgtcgttc tgcattatcg
     3301 gcattttcct gtgcggcgtc atcatcttcc gggtactctc ggaacagaaa tacgcggagc
     3361 aatcgcagag agagacagag gtcactctga gcgccgttac cgatgcagcg aatgaagcaa
     3421 tcgtcatggt gaatgctggc agcactgtta ccttctggaa ccccgcggcg gaaaaaatat
     3481 tcggctattg
//
LOCUS       DASSLD010000549         3690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1345567, whole genome shotgun sequence.
ACCESSION   DASSLD010000549 DASSLD010000000
VERSION     DASSLD010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345567"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..573)
                     /locus_tag="VFK23_09935"
     CDS             complement(52..573)
                     /locus_tag="VFK23_09935"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HET7319444.1"
                     /translation="MRLRIAGLAPALFFVLLLRPEAATADGFFVASGPGILHSRDSVA
                     VFLRYQRDAPALFGQAGFYEASYGWWSGKNRNEAISLARGLLWPAGEHGYLSFTAGLG
                     VVRTTTRNLDTPVEFLLRGAYGRRIGRFDISLGFTHFSNGKLFFRRIHPSWWDRGPNF
                     GENFPTLQVGVTF"
     gene            923..2545
                     /locus_tag="VFK23_09940"
                     /pseudo
     CDS             923..2545
                     /locus_tag="VFK23_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015124141.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
     gene            2610..3179
                     /locus_tag="VFK23_09945"
     CDS             2610..3179
                     /locus_tag="VFK23_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319445.1"
                     /translation="MNIVERMDRQPRKYKITVGILLALLTVLLDYWTGFGYRMKIFYL
                     VPISYVTWYVGKMYGTTFSFAAVMTMLNPRMASGTNSDGVTAIIWNMAAYFAFFVIVI
                     LLLSKLRSTMQQLEGFITKMQNALNAVQELSGILPICANCKKIRDDEGYWHDVDVYIS
                     RHTKAEFTHGICAECANKLYPSLFDKMGK"
     gene            3223..3678
                     /locus_tag="VFK23_09950"
     CDS             3223..3678
                     /locus_tag="VFK23_09950"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HET7319446.1"
                     /translation="MRWAVLLIFGTIGLAALAGGTIWGLEVYPVVRDSVSARGKVVEA
                     GVERSTAASPAASFPLVEFTTAGGVKVRFRARTGWESSPGHEVGDEVDVLYDPRNPDD
                     ARIGSVGQLWAGPLTTGGLGLLLLALSLLLFVKIGRFEKGIKSVGAGKK"
BASE COUNT          846 a         1031 c         1059 g          754 t
ORIGIN      
        1 gggaatgaca tcctatttgt tattgatcgt attgtgaaat tgtcaacaga ctcagaacgt
       61 cacccccacc tggagcgtcg gaaaattctc gccgaagttg gggccgcggt cccaccagga
      121 aggatggata cggcgaaaaa aaagcttccc gttcgaaaag tgcgtgaaac ccagggaaat
      181 gtcgaacctc ccgatccggc gaccgtatgc gcccctgagt aggaattcga cgggcgtgtc
      241 gaggtttctc gtcgttgtgc gcaccacgcc gagccctgcc gtgaaactca agtagccgtg
      301 ctctccggca ggccagagga gcccgcgcgc cagcgagatc gcttcattcc ggttctttcc
      361 gctccaccat ccgtaggagg cctcgtaaaa acccgcctgc ccgaacaatg ccggcgcatc
      421 tctctggtac ctcaggaaca cagcgacgga gtcccgggag tgcagaatgc ccggtcctga
      481 tgccacgaaa aaaccgtccg ctgtcgctgc ttccggccgg aggagaagga caaagaaaag
      541 ggcgggggca agacctgcaa tccgtagtcg catggaggcc tcatggcggt tccgggaacg
      601 agactcccgg acatctcgaa ggtagcacgc cggacatccc ctgtcaacgt cgccgcgcgg
      661 cagaccgccg ccccggcaat tgatttgttc cgtaaagtgt gatattaatc aactattatc
      721 acatgcgtga cgatgtatcc ggcatcgttc atccgcatta tcatgataaa gcagtaaaag
      781 gcggatttaa ccgcagagtc acagagagcg cggagaaaac ctttaattat tcttaaagct
      841 tttctctgcg actccgcgtc tccgcggtgg atttaaattt attaggagag cattattacc
      901 ttacggggga gggaccgcca ggatgaaggc ctcggacctg ctcgtcaaat gccttgaaaa
      961 tgaaaatgtc gagtatatct tcggcgtccc cggctaagaa acacttgacc taatggagtc
     1021 gctccggacc tcgaagatca aatacattct cacccggcat gaacaggtgg ctgccttcat
     1081 ggccgacgcg tacggcaggt tcaccggcaa ggccggcgtc tgcatgtcga ccctcgggcc
     1141 gggtgcaacc aacctcatga cgggcgttgc cgacgcctat ctcgacgcat cacccgtggt
     1201 tgcgatcacg gggcaggcgg acctcaagcg catccacaag gaattgcacc agtacatcga
     1261 catcgtcgac atgtgcgaga gcgtgaccaa gtggaatggc cggatcgagt cacccgaggt
     1321 catccccgag atggtccgga aggccttcaa actcgcggaa gcggaaaagc cgggtcccac
     1381 gcacctggag cttcccgaga atatcgccgc catgatgacg aaggagatgt atgtcgcgat
     1441 gatcccgccc atcagggccc ggcggagttc accggaccgc cagtccctcc ggaaggcggc
     1501 ggacctgata gaggcggcaa gctttcccgt gatcctggca ggccacggag tcgtgcgaaa
     1561 agccgccaac gagcagctgc gtcagttcgc cgaactgctc tgcatccccg tggtcacgac
     1621 cttcatgggg aaaggggcga tcagcgccaa gagcccttcc tgcatcggct ccctcggact
     1681 ttccggcgac cgccagatag ctgacgtctt cagccgggcc gacctcattc tcgccgtcgg
     1741 gtacgggctc gtcgaatacg ccccgaagaa ctggaatccg acgatcgaca aaaagatcgt
     1801 ccatatcgat ttcacctcga gcgaggtcga cctccattat gtacccgaag tcgagatcgt
     1861 ttccgacatc cgggaaagcc tggagctcct cgagggcatg ctcagcacga acaagaagag
     1921 ccggtgcgcc atcgatcttc acgccagttt cagcgaagaa ttcgaacgca tcgggtcgcg
     1981 gaagacctgg cccgtccgtc cgccgcagat cctgtacagg atcaggaagg tgctctccga
     2041 ttccgacatc ctggtctccg acgtgggcac atgcaaattc tgggcggcaa aattctatcc
     2101 cgtgtatgac aacaacacct tcatcctttc caacggcttc gcgtccatgg gcttttccct
     2161 tcccacggcc atggtggcca aactcgtgca cccggaccgc agggtggtcg ccctgtccgg
     2221 cgacggaggc ttcctgatga acctgcagga cctcgagacc gcggtgcgga tgaaactggg
     2281 catggtcatc gtcatcttca acgatcagag ctacaatctg atcaaatgga aatcgatgaa
     2341 aaagttcggc acgtcctacg gcgtcgattt caagaacccc gatttcatca aggtggcaga
     2401 aggcttcggc gcagtcggct tgcggctcga gtcggccgaa gacttcgagc ccatgctccg
     2461 ggaggccctc acgagggacg tgcccgtcat catagacgtg cccatcgatt attcggacaa
     2521 cgagctgatc tataaactgc tttgagttcg ggcctccgga atgggcatct tcgacgggga
     2581 gcctcttaag agagcaggcg gggtgtgata tgaacatcgt ggaacggatg gataggcagc
     2641 caagaaaata caagataacc gtcggcatcc ttctggccct gctgacggtt ttgcttgatt
     2701 attggacggg gttcgggtat cgaatgaaaa tattttatct cgtgccgatt tcctacgtaa
     2761 cctggtatgt tggaaagatg tacggcacta cgttctcatt cgcggctgtc atgaccatgc
     2821 tgaatccgcg catggcctcc gggacaaata gtgatggtgt tacggccata atctggaata
     2881 tggcggcata cttcgctttt ttcgtgatcg tgatcctgct cctttccaaa ttgcggagca
     2941 cgatgcagca gctggaaggt ttcattacga aaatgcagaa tgctttgaac gcggtgcagg
     3001 aactgagcgg catccttccc atttgcgcca actgcaagaa aatacgggac gatgaaggat
     3061 attggcacga tgtggatgtc tatatcagca ggcacacgaa agccgaattc acccatggta
     3121 tctgcgcgga atgcgcaaac aaactctatc cctcgctgtt cgacaagatg ggaaaatgag
     3181 tctgcagacc aggatttgcc ggagaaaagg aagaatgaat ccatgagatg ggcagtgctg
     3241 cttattttcg ggacgatcgg tctggccgcc cttgcgggcg ggaccatctg ggggcttgaa
     3301 gtatatcccg ttgtccgcga cagcgtgtct gcccggggta aggtcgttga agcgggagtg
     3361 gaaagaagca ctgccgcttc cccagcggct tctttcccgc tcgtggaatt tacaaccgca
     3421 ggaggtgtta aggtgcgctt ccgcgcgcgc acgggttggg aaagctcccc cgggcatgag
     3481 gtcggagacg aggtcgatgt cctttatgac ccccggaatc ccgatgatgc acgcatagga
     3541 agcgtcgggc aattgtgggc gggacccttg acaaccggtg ggcttggact cctcctttta
     3601 gcgttgagtc tgctgctctt cgtaaagatc ggacgcttcg agaagggaat caaatcggtg
     3661 ggcgccggga aaaagtaaaa accgttagtc
//
LOCUS       DASSLD010000550         4914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1284434, whole genome shotgun sequence.
ACCESSION   DASSLD010000550 DASSLD010000000
VERSION     DASSLD010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284434"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /gene="thiC"
                     /locus_tag="VFK23_09955"
     CDS             <1..971
                     /gene="thiC"
                     /locus_tag="VFK23_09955"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HET7319447.1"
                     /translation="VGTVPIYQAAVETVEQGKAIVQMDPDLLFRVIEAQAEEGVDFVT
                     VHCGVTLSSVERLKSQGRIMDVVSRGGAFHLEWMIFNEAENPLFTQFDRLVEIAKKFD
                     VTLSLGDGMRPGCLADATDRAQIEELITLGELRDRAHEAGVQVMIEGPGHVPLNQVET
                     NMRIQKELCKGAPFYVLGPLVTDVAPGYDHITSAIGGAIAGAAGADFLCYVTPSEHLR
                     LPDLEDVREGVIASRIAAHAADIAKGVKGALDWDREMARRRKALDWKGQIELSINPDR
                     ARRLRESSMPKESDVCTMCGEFCSMKGVSQYLKKDGRSQKSEVRKK"
     gene            991..1902
                     /locus_tag="VFK23_09960"
     CDS             991..1902
                     /locus_tag="VFK23_09960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7319448.1"
                     /translation="MRNAECGLEKKKGRYGSVILSILALIVTAVMLPAHAGAKAPQLK
                     VTLSPKKVGPGDILLVTVSGATGTVEGSFNGKRIFFNPSGHSFKAIVGIDLLSEPGKY
                     NLEINSTGATLRRPVKVSKKAYRTQRLKMPKDMVELSPENEARAERESKKMAAIWPNQ
                     TERLWSGNFMNPREGEIGTEFGVKRLMNNIPKNPHTGVDVVAEEGEEVRAPNNASVAL
                     VDEQFFSGNSVILDHGQGIYSMFFSSVQGPRRARTEGKEGRCYRARGGYRPRLGRPSP
                     LGRSRAGREGRPARADQIKAGIATHDS"
     gene            2028..2795
                     /gene="thiD"
                     /locus_tag="VFK23_09965"
     CDS             2028..2795
                     /gene="thiD"
                     /locus_tag="VFK23_09965"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET7319449.1"
                     /translation="MIKVLSIAGSDPSSGAGIQADLKTFQALGAFGMAIPAALTVQNS
                     RGVSDVEPVSSEMLSRQLDELLSDIKPYAVKTGMLLTKRNVDAVAKALSAFKVKNLVI
                     DPVLRSSSGRPLLRPDAVASLKKNLLPHALIVTPNIPETEVLSGMTIRSGKDMDFAAG
                     RILDLGPAYVLIKGGHRKGPAADTLYGGKTVLEFSTPRRDGEFHGTGCALSSAITVFI
                     ARGLSVEKAVEKAKQFVDSLLKTAKPVGKSRTKYFQF"
     gene            2880..3587
                     /gene="rph"
                     /locus_tag="VFK23_09970"
     CDS             2880..3587
                     /gene="rph"
                     /locus_tag="VFK23_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET7319450.1"
                     /translation="MRVDGRKADELRRVKITRNYIKSAEGSVLIEMGDTVVICTATVE
                     NTVPPFLKGQGTGWVTAEYAMLPRSSAQRIQRERGKVGGRTYEIQRLIGRSLRSIVDM
                     TALGERSILIDCDVVQADGGTRTASITGAYVALADALRLMKKQGLIDKVPLMDHLAAV
                     SVGIVDGKPLLDLCYAEDSEADVDMNLVMTGKGAIVEVQGTAEGEPFSKAELGVLLAL
                     GEKGIRALIRKQKEAFR"
     gene            3584..4144
                     /locus_tag="VFK23_09975"
     CDS             3584..4144
                     /locus_tag="VFK23_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019000083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HET7319451.1"
                     /translation="MTSFLTSLAIVLLAEMGDKTQLLAMAFATRFRWQTVMLGVFAAT
                     AANHLFAVYVGNFLTSIIPLHYIKLAAAVSFIIFGLWTLRGDEIGDEDKRFNFSPLWT
                     VIIAFFLAEMGDKTQLATVALAAKFNTIVPVWLGTTAGMMVADAIGIIAGIVLHKKIP
                     EKQIKWFAALIFIAFGLWGMYDAVGR"
     gene            4367..4585
                     /locus_tag="VFK23_09980"
     CDS             4367..4585
                     /locus_tag="VFK23_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7319452.1"
                     /translation="MAQYPVIDADQLKSRLHGKQKVALIDARPEDEYQQGHIPGAINI
                     RSDRMKEEAARLPKDKTTALIFYCRGEG"
     gene            4598..4723
                     /locus_tag="VFK23_09985"
     CDS             4598..4723
                     /locus_tag="VFK23_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319453.1"
                     /translation="MAAGAAMEMGYTNIMIYQAGMPDWLSKGNPVQKGSKPGKLK"
     gene            4779..>4914
                     /locus_tag="VFK23_09990"
     CDS             4779..>4914
                     /locus_tag="VFK23_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7319454.1"
                     /translation="MVMKREKANYTIQSVSHALDILESFTRTEDELGVTELSKRLALH
                     K"
BASE COUNT         1135 a         1351 c         1465 g          963 t
ORIGIN      
        1 ccgttggtac cgtgccgatc taccaggccg cggtcgagac cgttgaacag ggaaaggcga
       61 tcgtccagat ggaccctgac ctcttgttca gggtcattga ggcgcaggcc gaagagggtg
      121 tggacttcgt gaccgtgcac tgcggcgtga ccctttcgag cgtggagcgg ctcaaaagcc
      181 agggcaggat catggatgtg gtctcccgag gaggggcctt ccatctcgaa tggatgatct
      241 ttaatgaggc cgagaacccg ctgttcacgc agttcgaccg gctcgtcgaa attgcgaaga
      301 agttcgacgt gaccctttcg ctcggcgacg gcatgcggcc gggctgcctt gccgacgcca
      361 cggaccgggc ccagatcgaa gaactgatca ccctcgggga actgcgcgac cgggcgcatg
      421 aggcgggcgt gcaggtgatg atcgaaggcc cgggccatgt gccgctgaac caggtagaga
      481 cgaatatgag gatccagaag gaactctgca agggggcgcc gttctacgtg ctggggcccc
      541 tcgtgaccga tgtcgcgccg ggctacgatc atatcacctc ggccatcggc ggggcgatcg
      601 ccggggctgc gggcgcggat tttctctgct acgttactcc gtcggagcac ctccggctcc
      661 cggacctcga agacgttcgc gagggcgtga tcgcgtcccg gatagcggcc cacgccgccg
      721 acatcgccaa gggcgtgaag ggcgccctgg actgggaccg ggagatggca aggcgccgga
      781 aggcgctcga ctggaagggc cagatcgagc tttcgatcaa tcccgaccgt gcgcgacggc
      841 tccgcgaatc gagcatgccg aaggagtccg acgtctgcac catgtgcggc gagttctgtt
      901 ccatgaaggg cgtgagccag tacttgaaga aagacggcag aagtcagaag tcagaagtaa
      961 gaaaaaagta gaacagagag gaattgcgga atgcggaatg cggaatgcgg gctggaaaaa
     1021 aagaagggac ggtatggaag cgtcatcctt tcgatactag cgctcattgt cacggccgtc
     1081 atgctgccgg ctcatgccgg tgcgaaggcc ccgcagctca aagtgacatt gtcccccaag
     1141 aaggtcggac ccggagacat cctgctggtg accgtatcag gcgccacggg gacggttgaa
     1201 gggtccttta acggcaagag aatctttttc aatccctccg ggcattcctt caaggcgatc
     1261 gtcggaatcg acctgctgtc cgagcccggt aaatacaacc tcgaaatcaa cagcaccggc
     1321 gcaactctga ggcgtcccgt gaaggtgtcg aaaaaggcct accggaccca gcggctcaag
     1381 atgccgaagg acatggtcga actctcgccg gagaacgagg cgcgcgccga gcgggagtcg
     1441 aagaagatgg ccgcgatttg gccgaaccag acggagcgcc tctggtcggg caatttcatg
     1501 aatccgagag agggggagat cgggactgag ttcggcgtca agcgtctgat gaacaacata
     1561 ccgaaaaacc cgcataccgg cgtcgacgtc gttgccgagg aaggcgagga agtgcgcgcg
     1621 ccgaacaacg caagcgtcgc gctggtcgat gaacaattct tctcgggcaa ctccgtcatc
     1681 ctcgaccacg gccagggcat ctacagcatg tttttttcat ctgtccaagg tcctcgtcga
     1741 gcccggacag aaggtaaaga agggcgatgt tatcgggctc gtgggggcta ccggccgcgc
     1801 ctcgggcgcc catctccact ggggcgttcg cgtgcagggc gcgagggtcg acccgctcga
     1861 gctgatcaaa ttaaagctgg aatagctact cacgatagct gaaattccaa ataacaaaac
     1921 ccagatttca aataagctcc aataataaaa attcaaatag tttggatatt cgtttcttaa
     1981 tatttggaat ttatttgtga tttgttgctc gggatttggt gcttgccatg ataaaggtcc
     2041 tctccatagc cggttccgac ccgtcctccg gcgctggtat ccaagcagac ctcaagacct
     2101 ttcaggcgct cggcgccttc ggcatggcga tacccgctgc gcttacggtc cagaacagcc
     2161 ggggcgtgtc ggacgtcgaa cccgtgtctt cggaaatgct gtcgcgtcag cttgatgaac
     2221 tgctgtccga cataaaaccg tacgcggtca agaccggcat gctcctcaca aagcgaaatg
     2281 tcgacgctgt tgcaaaggcc ctttcggcat tcaaggtcaa aaacctcgtc atcgatcccg
     2341 tactccgttc cagttcggga agacctctgc tccgaccgga cgccgtcgct tccctcaaga
     2401 agaatctatt acctcatgcc cttatcgtaa cgccgaatat ccccgagacc gaggtcctct
     2461 caggcatgac aatcagatcg ggcaaggaca tggattttgc agccggcagg atcctcgacc
     2521 tggggccagc ctatgttctc atcaagggcg gccacagaaa gggtcctgcg gcggacaccc
     2581 tctacggcgg caaaaccgtg cttgagtttt ccacgccgcg ccgcgacggc gagttccacg
     2641 gcaccggatg cgcgctttca tcggcgataa ccgtgttcat tgcccgtgga ctgtctgtgg
     2701 aaaaggctgt ggaaaaagcc aaacaatttg tggacagctt gttgaaaaca gcgaaaccgg
     2761 tagggaagag caggacgaag tatttccaat tctaaagctt aaccacggag ccacggtgtc
     2821 acggagaaca aagatgttga ttctctctgc gtcctctgcg gtaaattcat ggagggttta
     2881 tgcgcgttga cggcaggaaa gcggacgaac tccgcagggt caaaatcacc aggaactaca
     2941 tcaagtccgc cgagggctcg gtattgatcg agatgggcga caccgtggtc atctgcacgg
     3001 ccacggtgga aaacaccgtg cctccctttc tcaaggggca gggtacgggc tgggtgacgg
     3061 ccgagtatgc catgctgccg cgctcgtcgg cccagaggat ccaaagggag aggggcaagg
     3121 taggaggccg cacgtatgag atccagcggc tcatcggcag gtcgctccgc tcgatcgtgg
     3181 acatgacggc gctcggcgag cgaagcattc tgatcgactg cgacgtggtc caggccgacg
     3241 gaggcacccg caccgcatcg atcacaggcg cctatgtagc gcttgcggac gcgctccggt
     3301 tgatgaaaaa acagggattg attgacaagg tacccctcat ggaccatctg gccgcggtga
     3361 gcgtcgggat cgtcgacggg aagccgttgc tcgatctctg ctacgcggag gactcggagg
     3421 ccgacgtgga catgaacctc gtgatgaccg gcaaaggcgc catcgtcgag gtgcagggca
     3481 cggccgaggg agaacctttt tcgaaggcgg aactgggcgt gcttctcgcc ttgggcgaga
     3541 aaggcatcag ggcgctcatc aggaagcaga aagaagcctt ccgatgacat catttctcac
     3601 gtcgcttgcg atcgtactcc tggccgaaat gggcgacaag acgcagctcc tggccatggc
     3661 tttcgcgacc cggtttcggt ggcagaccgt catgctcggg gtgtttgcgg cgaccgctgc
     3721 caaccatctg ttcgccgtat acgtcggcaa ctttctcacg agtatcatcc ccctgcacta
     3781 catcaagctc gccgcagccg tttctttcat cattttcggg ctctggacgc tccgcggcga
     3841 cgagataggg gatgaggaca aacgcttcaa cttcagtcct ctctggaccg tcatcatcgc
     3901 cttttttctg gcagagatgg gagacaagac ccagcttgcg accgtcgcac ttgccgccaa
     3961 gttcaatact atcgttccgg tatggctggg cacgacggcg gggatgatgg tagcggacgc
     4021 aatcggcatc atcgccggca tcgtgctgca caaaaaaatc ccggaaaagc agatcaaatg
     4081 gttcgctgct ctcatattca tcgcttttgg gctgtggggg atgtatgacg cagtaggtag
     4141 gtaagagctc aaatcgtcaa accgctcatt ggtcaacact tcttcagact tgtctcatct
     4201 cttacacgcc aataaaaata gagtttcgtt tttccttgac aaacagcaca acgcgaatta
     4261 atattaaacg cttgttttaa aatgacttaa ggagctacta cccatgacga aaccgaaaat
     4321 gttattgttt ttattggcat ttatgttctt tggttcaaca tcggccatgg cccaatatcc
     4381 cgttatcgac gcggaccagc tcaagtcccg tctgcatgga aagcagaaag tggccctcat
     4441 cgatgcaaga cccgaagatg aatatcagca gggacatatc cccggcgcga tcaacatccg
     4501 gtcggaccgg atgaaggagg aggccgcgag actgcccaag gacaaaacga cggcccttat
     4561 cttttactgc aggggagagg gctgaggtct tagccgtatg gccgcaggtg cggccatgga
     4621 gatggggtat acgaatatca tgatttacca ggcaggcatg ccggactggc tgtcgaaggg
     4681 caacccggtg cagaagggca gcaaaccggg gaaactgaaa taaagatatt tacctgaaat
     4741 ggcattacct tttctgacga aaggaaccgg cagaacccat ggtcatgaaa cgggaaaaag
     4801 caaactatac gatccagtcc gtgtcgcatg cgctcgatat tctcgaatcc ttcacgcgca
     4861 ccgaggacga actcggcgtg acggagctgt cgaagcgcct ggccctccat aaga
//
LOCUS       DASSLD010000551         2660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1054482, whole genome shotgun sequence.
ACCESSION   DASSLD010000551 DASSLD010000000
VERSION     DASSLD010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054482"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VFK23_09995"
     CDS             complement(<1..650)
                     /locus_tag="VFK23_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7319455.1"
                     /translation="MNKILGKSLHSPAMIAVLAAQFFSALADNAVLIVAIALVRSNGA
                     AGSVPLLQEAFVVPFLLLAPFAGPLADGFPKGRIMLLTNLLKFFGAAAMTAGLNPVIA
                     YGMIGIGATLYSPAKYGILTQLVEPERLVQANGMLEGSTIAAILLGVLMGGWLTDHAI
                     SWAFAGVIASYLVAAVANLFIPRLEPERAIKSIRPRVLTADFVTSLRTLFSNPGAR"
     gene            complement(647..>2660)
                     /gene="aas"
                     /locus_tag="VFK23_10000"
     CDS             complement(647..>2660)
                     /gene="aas"
                     /locus_tag="VFK23_10000"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HET7319456.1"
                     /translation="LDGLFLGLFLPYDPVFVVHTYVTRNRFLRLVLSQVDHLAVDPAN
                     PMAIKKVIKLVEEGRPVVIFPEGRITVTGSLMKVYAGPAFVAAKTGATILPVRLDGPA
                     RSWFSRLSNRPRRRLFPRVTLSIQPPAAIAMPEGETARLRRHKAGEAMRRIMQKMLCE
                     SEPKRTLYAGLLDAISVHGQRRRVVEDMRQVEYSYHDLLKMTLALSRMAAKNSAPGEC
                     IGILLPNLASTVCMVIGSTALGRVPALVNYKAGARGIRDACVAARIKTLFTSRAFIEQ
                     AKIEGDLAALPAVTILYLEDLRERIGLCDRLWILWGMLFPRTVEKRRNPESAAVVLFT
                     SGSEGRPKGVVLSHRALMSNIAQIRSVIDFAADDKVLNALPVFHSFGLTAGALLPIMT
                     GMNLFLYPSPLHYRVIPELVYDRSCSILFGTNTFLANYAKYAHPYDFYRLRYVVAGAE
                     KLSESVRGLWFEKFGIRILEGYGATETAPVLAVNTPMAYKTGTVGQFLPAVEYRLDPV
                     PGIKAGGMLSVRGPNVMSGYFRADAPGRLDPPRTGAGEGWYETGDVVSVDDDGFISIV
                     GRVKRFAKVAGEMVSLEMVERVAALAAPSSLHGVSARQDGARGEAIVLFTTDKSLSRD
                     ALVDSARRLGVPELAVPRKILRVDSLPLLGTGKIDYVALKQLAEAI"
BASE COUNT          548 a          798 c          844 g          470 t
ORIGIN      
        1 aagcgtgcgc cgggattgga gaacaaagtc ctgagcgaag ttacgaaatc cgccgtcaac
       61 acgcgcggac ggatagactt tatcgcccgt tccggctcca ggcgtgggat gaacaggttt
      121 gccaccgcag caacgaggta cgaagcgatc actccggcga aggcccatga gatggcgtga
      181 tcggtgagcc agcctcccat taatacgccg aggaggattg ccgcgatcgt cgatccttcg
      241 agcattccgt tcgcctgtac gaggcgctcg ggctcgacca gttgcgtcag aatcccgtac
      301 tttgccgggg aatagagcgt ggcgccgata ccgatcattc cgtacgcaat gacagggttc
      361 aggcccgcgg tcatcgccgc agctccgaag aacttcaata agttggtcag gagcattatc
      421 cgtcccttgg gaaagccgtc ggcaagcggg ccggcaaagg gggccagaag caggaagggg
      481 acgacaaagg cctcctgcag aaggggcacg ctgcctgcgg ccccgttcga tctgaccagg
      541 gcgattgcga cgatcagcac ggcgttgtcc gcaagggcgg aaaagaactg ggcggccaga
      601 acggcgatca tggccggcga gtgcaaagac tttccgagga tcttgttcat atcgcctccg
      661 ctaactgctt taacgcgacg tagtcgattt tgcccgtgcc aagaagcgga agggaatcga
      721 ccctgaggat cttccggggc accgcaagct cgggcacgcc gagcctgcgg gcggaatcga
      781 ccaatgcgtc gcgtgaaagc gacttgtcgg tggtgaagag aacgattgcc tcgccccgcg
      841 cgccgtcctg ccgggcagat acgccgtgca gtgaagaggg agcggcaagc gctgctaccc
      901 gctcgaccat ctcgagggat accatttctc ccgcaacctt ggcaaagcgt ttgacgcggc
      961 cgacgatgga gatgaacccg tcgtcgtcca ctgatacgac atcaccggtc tcgtaccatc
     1021 cttcgcctgc ccccgtccgc ggcggatcga gcctgcccgg cgcatctgcc cggaagtagc
     1081 cagacattac gttcggtccg cgaacagaga gcattccgcc ggcctttatt cccggcacgg
     1141 ggtccaggcg atattcgacc gcgggcagga actgtccaac ggtcccggtt ttataggcca
     1201 tgggggtgtt aaccgcaagg accggcgccg tttcggtcgc cccgtaccct tccaggatgc
     1261 ggatgccgaa cttctcgaac cagaggccgc gaacagactc ggacagcttc tccgccccgg
     1321 cgaccacgta ccggagccgg taaaaatcgt agggatgagc gtacttcgcg taattcgcaa
     1381 ggaaggtgtt cgttccgaag aggatgctgc atgaccggtc atagacgagt tcgggaatga
     1441 cgcggtagtg caagggggag ggatagagga acaggttcat gcccgtcatg atggggagca
     1501 gcgcgccggc ggtgaggccg aaagaatgga agaccggcag cgcattcagc accttgtcgt
     1561 cggcagcgaa atcgatcacc gagcggatct gcgcaatgtt ggacatgagc gccctgtggg
     1621 acagcacgac gcccttcggc cttccttccg atccggacgt gaagagcaca actgcggcgc
     1681 tttcgggatt cctgcgcttt tcgacagttc gcgggaagag catgccccaa agtatccaga
     1741 gtctgtcgca gagtccgatg cgctcccgaa ggtcttccag gtaaagaatg gtgacggccg
     1801 gaagtgccgc aagatcgccc tcgatttttg cctgctcgat aaaggccctc gaggtaaaca
     1861 gggtcttgat ccgcgcggca acgcatgcat cccgtattcc ccgtgcgccc gccttgtagt
     1921 tcaccagggc agggacccgg cccaaagcgg tcgagccgat gaccatgcag accgttgatg
     1981 cgaggttcgg cagcaggatc ccgatgcatt cccccggagc gctgtttttc gcggccatgc
     2041 ggctcagggc aagcgtcatc ttgagaagat catggtagga atactcgacc tgcctcatgt
     2101 cctccaccac gcgacgccgc tgtccatgaa ccgagatcgc gtccagaagt cccgcgtaca
     2161 gagtgcgctt cggctcggac tcgcacagca ttttctgcat gatgcggcgc atcgcctcgc
     2221 ccgccttgtg acgccgaagc cgcgccgttt caccctccgg catggcgatc gcggcgggag
     2281 gctggatcga gagcgtgacg cggggaaaca ggcgacgccg gggccggttg gagaggcggc
     2341 tgaaccatga gcgggccggt ccgtccaacc ggaccgggag gatcgtggcg cctgttttcg
     2401 cagccacgaa cgcgggaccg gcgtagacct tcatcagcga gcccgttacc gtgatcctgc
     2461 cttcgggaaa gatcacgacg ggacgtccct cttcgaccag cttgatcacc ttttttattg
     2521 ccatgggatt ggcagggtcc acggccagat ggtcgacctg gctcaggacc agccgcagga
     2581 agcggtttcg ggtcacgtag gtatgcacca cgaacaccgg gtcataggga agaaacagtc
     2641 ccaggaacag tccgtccagg
//
LOCUS       DASSLD010000552         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1532462, whole genome shotgun sequence.
ACCESSION   DASSLD010000552 DASSLD010000000
VERSION     DASSLD010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532462"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VFK23_10005"
     CDS             complement(<1..727)
                     /locus_tag="VFK23_10005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET7319457.1"
                     /translation="MSLFHSHSGNRSHDHGHDGHDHHEHELEQGPHRVVPPGGKRDLL
                     IALSITMLMMVAEVIGGLLSNSLALLSDAGHMLTDNLALLLSFFAMKFATLPATERKT
                     WGFYRLEILAALLNGIVLVLLSLYIMYEAYLRMLHPQPVQGTLMLVVAVIGLVANIIG
                     ALILIRHRDTNLNIRGAFLHIVGDALSSVGVIMGGVIILYTGWYLIDPILSMMISLVI
                     IYGSWALVKESVNILLEAVPAHID"
     gene            complement(747..1565)
                     /locus_tag="VFK23_10010"
     CDS             complement(747..1565)
                     /locus_tag="VFK23_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HET7319458.1"
                     /translation="MLKLGHIVYSNCYPPHAGILTRKVAFPFKLVEGIPTQLNRLLYE
                     GGVDVSPSSSIEYAMNPGRYLLMPDLSITSKSRVMSILLESNVPIEELHDRVVALTTA
                     SATSVVLLRILLEVRYGAKPGYVLYEQGAEEPPEQAVAALTIGDLAIRKSGASKYAYR
                     YDLGRLWHDFTGLPFVFALWQVNDRKNVHKELERLYDILIESRAYGLAHLAEIAEAET
                     GRFNLPAPVLMEYWKLFSYELGEEERKGLLAFYGYAAEIGAIEAVTELRFWSKD"
     gene            complement(1566..>2042)
                     /locus_tag="VFK23_10015"
     CDS             complement(1566..>2042)
                     /locus_tag="VFK23_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7319459.1"
                     /translation="YMILASQFESFVHPFTIMFSLPVSLIGAMGLLFLTGERVQIFSL
                     IGVIMLMGLVTKNAILLVDYTITLRKRGMDRTEALLKAGPVRLRPIIMTTAAMVFGML
                     PTALKIGEGAESRAPMAITVIGGLITSTLLTLVVIPVVYTLVDDLENFFKRKRKKA"
BASE COUNT          426 a          564 c          649 g          403 t
ORIGIN      
        1 tgtcgatgtg ggcgggcacg gcctccagca ggatgttcac ggactccttg acgagcgccc
       61 aggagccata gatgatgacc agggagatca tcatgctcag gatcgggtcg atcaggtacc
      121 agccggtata caggatgatg acgccgccca tgatgacgcc gacggaggag agcgcgtcgc
      181 cgacaatatg gagaaacgcc ccgcggatgt tgaggttggt gtcccgatgc ctgatcagaa
      241 tgagcgcgcc gatgatgttg gcgacgaggc cgatgaccgc gacgacaagc atcagggtgc
      301 cctgcacggg ctgcggatga agcatcctca ggtaggcctc atacatgatg tagagcgaaa
      361 gcaggacgag gacgatgccg ttcaggagcg cggccaggat ctcgagccgg taaaagcccc
      421 aggtcttacg ctccgtggcc ggcagggtgg cgaatttcat ggcgaagaaa gacagcagga
      481 gggcaaggtt gtccgtcagc atgtgtccgg cgtcggacaa aagggcaagg ctgttggaca
      541 acaggccgcc gattacctcg gccaccatca tgagcatggt gatcgacaac gcaatcagaa
      601 ggtcgcgttt ccctcccggg gggactacac ggtgcgggcc ctgttcgagt tcgtgttcat
      661 ggtgatcatg accgtcgtgg ccatggtcat gtgaacgatt accgctgtgc gaatggaaca
      721 aagacatgat gaagcaggct ccttcctcag tcctttgacc agaaccgcaa ctccgtcacc
      781 gcttcaatag cgccgatttc cgcggcgtag ccgtaaaagg ccagcaggcc ctttcgctcc
      841 tcctcgccca attcgtagct gaacaatttc cagtattcca tgagcaccgg ggcggggagg
      901 ttgaacctgc ccgtttccgc ttctgcgatc tctgcgaggt gcgccaggcc gtaggccctc
      961 gactctataa ggatatcata caaccgttcg agttccttgt gtacgttttt ccggtcgttc
     1021 acctgccaga gcgcgaaaac gaagggcagg ccggtgaaat catgccagag ccttcccaga
     1081 tcgtaccggt aggcgtattt cgaggcaccg gacttccgta tggcaaggtc cccgatcgtg
     1141 agcgcggcga cagcctgctc cggaggttcc tcggcgccct gttcgtagag gacatatccc
     1201 ggttttgctc cataccggac ctccaggagg atgcggagca gcactaccga ggtggccgac
     1261 gccgtggtca gggcgacgac ccggtcatgc agctcctcga tggggacgtt gctttcgagg
     1321 aggatgctca tgaccctgct tttcgaggtt atcgagaggt ccggcatgag caggtagcgt
     1381 cccgggttca tggcatactc gatggacgag gagggagaga catccactcc gccctcgtag
     1441 agcagccggt tcaactgggt cgggatgcct tccacgagtt tgaagggaaa ggcgaccttt
     1501 cgcgtcagga ttccggcgtg gggtgggtag cagttggaat agacgatatg gccgagtttg
     1561 agcatttatg cctttttcct tttcctcttg aagaaattct ccaggtcgtc taccagcgtg
     1621 tatacgacgg ggatgacaac gagcgtcaac agcgtcgagg tgatcaggcc gccgatcacg
     1681 gtgatggcca tgggcgcccg cgattcggcg ccttcgccga tcttcagggc agtcggaagc
     1741 atgccgaaga ccatggccgc ggtggtcatg atgatcggcc ggagccggac cgggccggcc
     1801 ttgaggagcg cctccgtgcg gtccattccc cgctttctga gggtgatcgt gtaatcgacg
     1861 agcagaatcg cgttcttggt cacgagtccc atgagcatga tcacgccgat caagctgaat
     1921 atctgcaccc gttctccggt caggaacagg agccccatgg cgccgatgag gctgacgggg
     1981 agcgagaaca tgatcgtgaa cggatggacg aagctttcga actgcgacgc caggatcatg
     2041 ta
//
LOCUS       DASSLD010000553         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_391753,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000553 DASSLD010000000
VERSION     DASSLD010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391753"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VFK23_10020"
     CDS             complement(<1..904)
                     /locus_tag="VFK23_10020"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HET7319460.1"
                     /translation="MKYIILLGDGMADRPHAELGGKTCLQAARTPNLDQLASLGQVGM
                     VQTVPPGFPPGSDVANLTVMGYDPRKYYSGRSPLEAASIGVKLGPDDVAFRCNLVTLR
                     ITGGKSAGVHRKAVMEDFSAGPISTQEARLLIEEIESKLGNDRIRFYPGVSYRHLMVW
                     KGGKDRMECTPPHDIQDKDIQDYLPRGEGDETIAALMESSFDVLANHPVNKKRQEAGK
                     RTANSIWLWGQGKRPQMPTFREKYGLEGAMISAVDLTKGIGIYAGFEVVDVPGATGWL
                     DTNYVGKAEHALWALKTKDIAYVHV"
     gene            complement(1028..1270)
                     /locus_tag="VFK23_10025"
     CDS             complement(1028..1270)
                     /locus_tag="VFK23_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HET7319461.1"
                     /translation="MATKRLKLTFPRELIKEPVIFTMARKFDVMPNIRRAKIAENVGE
                     VVLELEGSESKLEEGIAFLRERGVKVEAIEEDSSDS"
     gene            complement(1387..1662)
                     /locus_tag="VFK23_10030"
     CDS             complement(1387..1662)
                     /locus_tag="VFK23_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET7319462.1"
                     /translation="MAIKVRIPTPLQKLTNGKTEVECSAKNIQELIDALEKDYPGIKD
                     RLSDGGKIRRFINIYVNEEDIRFVNKEATVLKDGDNVSIVPAIAGGC"
     gene            complement(1836..>2286)
                     /locus_tag="VFK23_10035"
     CDS             complement(1836..>2286)
                     /locus_tag="VFK23_10035"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET7319463.1"
                     /translation="RCVLCGQLSMLILKKSDMQAILDHCTAGFPNEACGILAGTVGRV
                     EKVYAMINAKPGPAYYEMDPEEQFRVLKDIRRAGLAMVGVFHSHPAGQAYPSRVDVEK
                     AYWPGTQLPNYPDTVQVIISLLDPAAPVAKGYSLLEGTISEVPLSVE"
BASE COUNT          450 a          707 c          605 g          524 t
ORIGIN      
        1 cgacatggac atatgcgata tccttggtct tgagggccca gagcgcatgt tctgccttgc
       61 ccacgtagtt tgtgtcgagc catcccgtcg cgccgggcac atcgaccact tcgaagcctg
      121 cgtagatacc gatgcccttc gtgaggtcca ccgccgatat catggcccct tcgagaccgt
      181 acttttccct gaaggtcggc atctgcggac gctttccctg gccccagagc cagatgctgt
      241 tcgccgtccg cttgcccgcc tcctgccgct ttttgttcac cggatggttg gcgagcacat
      301 cgaacgacga ttccatgagg gcagcaatcg tctcgtctcc ctcgccgcgc ggcagatagt
      361 cctggatgtc cttgtcctga atatcatgcg ggggagtgca ttccatccgg tccttgccgc
      421 ctttccagac catgagatgg cgatagctca cgcccggata aaaacggatg cggtcgttgc
      481 cgagtttgct ttcaatctcc tcgatcagca ggcgcgcctc ctgggttgag ataggcccgg
      541 cgctgaaatc ctccatgacc gccttgcggt gcacgcccgc ggactttccg cccgtgatcc
      601 gcagcgtcac gaggttgcaa cggaacgcaa catcgtccgg gccgagtttc acgccgatac
      661 tcgcggcctc gagcggcgaa cggccgctgt aatacttccg gggatcatat cccataaccg
      721 tgagattggc cacgtcgctg ccgggcggaa accccggggg gaccgtctgc accatcccca
      781 cctgccccag cgacgcaagc tgatcaaggt tcggcgtcct tgccgcctgc aggcatgtct
      841 tcccgcccag ttccgcatgc ggccggtcgg ccatgccgtc tcccagtaag atgatgtatt
      901 tcataaggcg gcctccggcc gcgaccaagt cagatattga caactggata ccggatactg
      961 gatgaaagcc acggagacct aatgccgatc gatttatcca gcatcaagca tccagcatcc
     1021 ggctcgatta agaatccgaa gagtcctctt cgatagcttc aaccttgacg ccccgctccc
     1081 tgagaaaagc gatgccctct tcgagcttgc tctcggaccc ttcgagctcc agcaccacct
     1141 cgcccacatt ctcggcgatt ttggcgcgcc ggatattcgg catgacgtcg aatttcctgg
     1201 ccatggtaaa aatcaccggt tccttgatca gttcccgggg aaaggtcagt ttgagcctct
     1261 tcgttgccat ggcgcctccg ttgtaaagat ggacgtcggg atggtgggac aatgagaggt
     1321 tgagagggag ataaacgata tcctcattct caccttctca cgctcccacc ttttcacctt
     1381 ctggcatcaa cagccccctg caatagccgg aacgatcgag acattatcgc cgtccttcag
     1441 tacggtcgct tccttgttca cgaaacggat gtcctcttcg ttaacgtata tattaatgaa
     1501 ccgtctgatc ttgcctccgt cggagagcct gtcctttatg ccgggatagt ccttctccag
     1561 tgcatcgatc aattcctgaa tgttcttggc gctgcattcc acttccgtct tgccgttcgt
     1621 gagtttctga agcggagtcg gaattcgaac tttaatagcc ataaatcaat cctccacgaa
     1681 gttgctgaat aaatccttgg tgtcattgcg aggagcgatt tttcgacgcg gcaatctcgg
     1741 tttttcacat cgtcatgaac tgcgagattg cttcgctgag cccgcaaaga cggcttaaca
     1801 gactgaactc gcccgcccgg atgcgaagtt tcatgctatt ccacggacaa ggggacctcg
     1861 ctgatcgttc cttccaggag ggagtaaccc ttggcgacgg gcgccgcagg gtcgaggagc
     1921 gaaatgatca cctgtaccgt gtcgggatag ttcggcaact gcgtccccgg ccagtacgcc
     1981 ttctccacgt ccacacggga aggataggcc tggccggccg ggtgggagtg gaacacgccg
     2041 accatcgcaa ggcccgcccg gcgaatatcc ttcaagaccc tgaactgctc ctccggatcc
     2101 atctcgtagt aagccgggcc cggtttcgcg tttatcatcg cgtatacttt ttccaccctc
     2161 ccgacggtgc ccgcaaggat gccgcaggct tcattcggga aacccgcggt gcagtggtcg
     2221 agaatggcct gcatatcgga tttctttaat atcagcatcg ataattgacc gcagaggacg
     2281 cagcgg
//
LOCUS       DASSLD010000554         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_43641,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000554 DASSLD010000000
VERSION     DASSLD010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43641"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1221)
                     /locus_tag="VFK23_10040"
     CDS             complement(91..1221)
                     /locus_tag="VFK23_10040"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7319464.1"
                     /translation="MNVLIICTNRNKFPVPVLPSGACMVAEAAERAGHSVRLLDLMFE
                     ADPLRAIKAELGGASYDVIGLSVRNIDNVEMGGPRYFIDDLTSLIEAVRAETDAHVIM
                     GGTAVTVMPEEILRTTLISRAVIGDGVVVLPRLLERLARHELCNDLAGVASFDDGVFR
                     INSPANGPCDNCAAPDYRRWLNMKAYRSYLSTVPLQTKLGCRFQCVYCTYPKIEGDAY
                     RLMDPGSAAEAALRFASSGLRDIEFVDNVFNAPYDHAMAVCEALIRVRPRARFQSLEL
                     NPAVFDHELLSAMERAGFVGIGLTVESASDPVLRGLRKGFTAREVHAAAEVVRDHRLP
                     CLWIFMLGGPGETRETVRETLRFVETSIRPQDTALFNVGVRI"
     gene            1503..2285
                     /locus_tag="VFK23_10045"
     CDS             1503..2285
                     /locus_tag="VFK23_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET7319465.1"
                     /translation="MNAFFIMLPVVMVLAANPAFGHESTASKADVMLEERLGQSIPED
                     LVFTDEYGIRTRLKDCFGKPVIIAPVYLGCAHTCPLLLSGLAQTLGRLDLVKPGKDFT
                     VITLSFDEKDTPAVALEKKLNYLKAVSRPFPPETWKFLTGDAANIGKFTDAIGFRFQR
                     DGVDFSHPITLVVVSPRGKIVRYLQGVTFLPFDVTMAINEAAEGRIGSPARKVLTYCF
                     SYDPLKKSYVFNILKITATVMVLFVVSFLAYLVFASKKKRKT"
BASE COUNT          523 a          742 c          700 g          536 t
ORIGIN      
        1 agagacgtag aacaccggct cgagcatccc cgccggcgga acggcgagca ggccctccct
       61 gcgtgctatg gcctcgagct ccgtgccggg ctagatccgt acgccgacgt tgaaaagcgc
      121 cgtgtcctgc ggtctgatgg aggtctctac gaagcgcagc gtttccctta ccgtttcccg
      181 ggtctctccc ggccccccga gcatgaagat ccagagacac ggcagccggt ggtccctgac
      241 cacttcagct gcagcgtgga cctcccgcgc ggtgaagccc tttctgagcc cgcggagaac
      301 ggggtcggat gcgctctcga ccgtgagtcc gatccccacg aaccccgccc gttccatggc
      361 agagagcagt tcatgatcga agacagccgg gttcagctca aggctctgga agcgcgccct
      421 cggccgcacc cgtatgaggg cctcgcatac ggccatcgcg tggtcgtagg gggcgttgaa
      481 gacattgtcg acgaactcga tgtcacgaag gcctgaagac gcaaaacgga gggcggcttc
      541 ggcagcgctc ccggggtcca tgaggcgata ggcatcgcct tcgatctttg gatacgtgca
      601 gtagacacac tggaaccggc atccgagttt cgtctgaaga gggacggtgg acaaataaga
      661 gcggtatgcc ttcatgttca gccagcgccg gtaatccggt gccgcgcagt tgtcgcacgg
      721 tccgtttgcg ggcgaattta ttctgaagac gccgtcatca aaagaggcga cgcccgccag
      781 atcgttgcag agttcgtggc gggccagtct ttccagcagc cgaggcaata caacgacgcc
      841 gtccccgatc accgcccggg agatgagcgt tgtcctgaga atttcctccg gcatgacagt
      901 caccgcagtg ccgcccatga tgacatgcgc gtccgtctcc gcgcggacgg cttcgatgag
      961 gctcgtcagg tcgtctatga agtaccgcgg ccctcccatt tctacattgt cgatgttccg
     1021 caccgacagc cctatgacat cgtatgacgc tccgcccaac tccgccttga tcgcacgcag
     1081 agggtcggcc tcaaacatga ggtcaagaag ccttaccgag tgccctgctc tctccgccgc
     1141 ctcggcgacc atgcatgcgc ctgaaggcag tacgggcacc ggaaatttgt ttcgattcgt
     1201 acagatgatc agaacattca tacgcgggat gtctccggca aggaatgtgc cgggggatga
     1261 aacgcaacac cttgtttaat aatacaacgt tcattgggca tgtcaacccc gttgccgcat
     1321 taatcagccg acacatgaag agtacagttt cagttcacta tgagcgatat cgggtcaagg
     1381 cgggcaggtg aactttgctt gactttttcg taatatacga tatgctatcg gtaattcaag
     1441 gccttgtcgg agagatagtt tcatgccccg gacgacatca acgggacgtc caaggagccg
     1501 cgatgaatgc gtttttcatc atgctgcctg tagtcatggt tcttgccgcg aaccctgcct
     1561 tcggtcacga gtccacggcc tcgaaggcgg acgtcatgct ggaagaaagg ttagggcagt
     1621 ccatcccgga agacctcgta tttaccgatg agtacggtat ccggacaaga ctcaaggact
     1681 gcttcggaaa acccgttatc atcgcgccgg tctatcttgg ctgtgcgcac acgtgccccc
     1741 tgctcctgtc cggcctggca cagacactcg gccgtctcga tctggtcaag ccgggaaagg
     1801 acttcacggt tatcacgttg agctttgatg agaaggatac gccggccgtt gctctggaaa
     1861 agaaactgaa ttatctcaaa gcggtgtccc ggcctttccc gccggagacg tggaagttcc
     1921 tgaccggcga cgccgcgaac atcggcaaat ttaccgatgc catcggcttt cggttccagc
     1981 gggacggtgt cgatttttct catcccataa cgctcgtcgt cgtttccccc cggggaaaga
     2041 tcgtccggta ccttcagggc gtgacctttc tgccatttga cgtcaccatg gccataaacg
     2101 aggcggctga aggaaggatc ggttcaccgg cccgtaaagt gcttacctat tgtttcagct
     2161 atgatcccct caagaagtcc tatgtgttca acatattgaa gataaccgcg actgtcatgg
     2221 tcctgttcgt agtatcgttc ctcgcttatc tcgtctttgc gtcaaaaaag aaacgaaaaa
     2281 cataatcagt ccccctcacc ctagcgtctc ccctcgggaa gaaaggcgag gggtagttcg
     2341 agtttggaga accggatcat ggaaacaagc ttctaccata caccgggcag ataccccggc
     2401 gtcctcggat ggatattcac caccgaccac aagcggatcg gccttctgta cctcggttcg
     2461 acgctcgctt tcttcgccgt gggtgtcttg ctcggcctcg t
//
LOCUS       DASSLD010000555         2335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_336439,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000555 DASSLD010000000
VERSION     DASSLD010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336439"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..584)
                     /locus_tag="VFK23_10050"
     CDS             complement(87..584)
                     /locus_tag="VFK23_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319466.1"
                     /translation="MATEKQKEAAKNNIKKAQAAWKAMSSTEHARSQPEGRARKKPGT
                     TGEGKYYRIVVRSKEDFVTFRYHDVGEPGGIQRLAGKRSSGSWDDQAWLISKEMAHTE
                     GNTLVADTPDAKKILQVIGPAKHVKGDIFQGHPRKNVPEREKPTPAQRRARMENIRKA
                     QQARR"
     gene            complement(599..742)
                     /locus_tag="VFK23_10055"
     CDS             complement(599..742)
                     /locus_tag="VFK23_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HET7319467.1"
                     /translation="MTISVSHLQPVAALIAGILILVIPRLLNYIVAIYLIIIGILGLA
                     HNM"
     gene            940..1173
                     /locus_tag="VFK23_10060"
     CDS             940..1173
                     /locus_tag="VFK23_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319468.1"
                     /translation="MKVRVICYEGYKAEETPRAFLLGERRVEVVRVLDRWRGEDHEYV
                     KLDGSDGARYILRYDRSREEWEIHLMESPPSSG"
     gene            complement(1235..1777)
                     /locus_tag="VFK23_10065"
     CDS             complement(1235..1777)
                     /locus_tag="VFK23_10065"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7319469.1"
                     /translation="MLVSQAGRKINRGGLQVAANSVNRPGELQARRLESVYEQLATLI
                     VHSDAIRLLRVAGVNEWSVLQVLGHMVEMIPYWLGHCRVMITTTGKPPVFGRGMDAPE
                     RLAGIERGSNGDPKELLGLMHDEVKAAASAIRDMSAAERSKRGIHIKLGELTVADCVE
                     RFIVAHSEDHLEQIRATLSG"
BASE COUNT          471 a          636 c          612 g          616 t
ORIGIN      
        1 ggcgtaagtc ccgttcgttg caacagcaaa agggaacttg gcttcgcgcc aggttccctt
       61 tttaggtctt gactctctct atctcactac cggcgcgcct gttgggcttt gcgaatattt
      121 tccattcgcg cgcgtctctg agcaggggtc ggcttttccc tttcaggaac attttttctg
      181 ggatgtccct ggaagatgtc tcctttaaca tgtttcgcgg gtccgatcac ctgcaatatc
      241 ttttttgcat cgggagtatc ggcgacaagc gtgttccctt ccgtgtgagc catctccttg
      301 ctgatcagcc aggcctgatc gtcccaggag ccgctggatc ttttccccgc caatctctgg
      361 atccctccag gttctcccac atcatggtat ctgaaggtta caaagtcctc tttcgatcga
      421 acgacgatgc gataatattt tccttctcct gttgtccccg gtttttttct tgcccgtccc
      481 tccggttgac ttcttgcgtg ctccgtcgat gacattgctt tccatgccgc ctgtgctttc
      541 tttatattat ttttcgcagc ttctttttgc ttttccgtag ccattggttt ttcctcccct
      601 acatattgtg agccagtccg aggattccaa tgatgatgag atagatggcg acgatataat
      661 tcaaaagcct tggaatgacg aggatcagga ttccggcgat caaggccgct acaggttgta
      721 gatgagagac gctgatggtc atgccgattc cgcctccttt atgagatttc cctttccttc
      781 tcggtaagac gagcatccgg ggcagtccgg tttcatgtgt cgagacggat tcgaggggtg
      841 gccctttttc gctggatcgt taaatatatg tgaaactggc tctggtgatc gggcaaggga
      901 ccgttggcga ggtaaccggt aaggggcggg gagcggggca tgaaggttcg tgtgatttgc
      961 tacgaagggt acaaggcgga ggagacgccc cgggcgttcc tgctcgggga gcgccgggtc
     1021 gaggtggtac gcgtcctgga ccggtggcgg ggagaggacc acgaatacgt aaagctcgac
     1081 ggctccgacg gcgcccgcta catcctccgg tatgatcggt cccgggagga gtgggagatc
     1141 catttgatgg aatcgccgcc atcttcggga tgatcaccct tgaaatcgaa acacactggg
     1201 aatcgtaatc gaataagcga cgaccctggg atggtcagcc cgacagcgtt gcgcgaatct
     1261 gctcgaggtg atcttccgag tgggcgacga tgaaccgttc cacgcagtcg gctactgtca
     1321 attcgcccag tttgatgtgg atgcctctct tgcttcgctc cgcggcggac atatcgcgga
     1381 tggcgctggc cgcggccttc acttcatcgt gcatcaggcc cagcaattcc ttgggatctc
     1441 cgttggagcc gcgttcgatc ccggccagac gctcaggcgc gtccattcca cgtccgaaaa
     1501 cgggaggttt tccggtggtg gtaatcatca cgcgacaatg ccccagccaa taggggatca
     1561 tctccaccat atgtcccaag acctgcagta ccgaccactc gtttacgcca gcgacgcgca
     1621 ggagccggat ggcatcggaa tggacaatta gcgttgccag ttgttcgtag acggactcca
     1681 accgccgggc ttgcagttcc cccggacgat tcacgctatt tgccgccact tgcaaccctc
     1741 cccgatttat tttccttccg gcttgggata ctagcattac atatccaaac ccaaaccgga
     1801 ggaattgagg tcagggtgtc gagccggttt tatccgaaga ttatcaggtg gggttgcgca
     1861 gaatgcttat ggaagaggtg aaatccgttt cctcatcgga tacggtccaa taaaaaccgc
     1921 aatccatgca attttgatat ttttttgcgg tagtcccatg aacctctccg aaacaaaatg
     1981 ttcccgcaat tgcccaacaa ctacgcccgc cgtttcttcc cccattgata ccattcgcgc
     2041 tgtcatccac ggcggcaggg cacacccctt tttcggctac acattcccca ccgggttctc
     2101 ggccgcattt tatgaattcc caacagttga ccttgcgctc tcgtttcatg accacgctac
     2161 agggggataa agtaggcgtt cctttttggc ccaagtcatt atatggaatg attattattc
     2221 accccggaat gaaatcgcaa gatatttcta ttacgttacg ttgtcagaag atatggcaat
     2281 tttcagggtc ggagtgcccc aacgctggtt gtttgacaag attcgaacga aataa
//
LOCUS       DASSLD010000556         3539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_632308,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000556 DASSLD010000000
VERSION     DASSLD010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632308"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..295
                     /gene="hfq"
                     /locus_tag="VFK23_10070"
     CDS             29..295
                     /gene="hfq"
                     /locus_tag="VFK23_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HET7319470.1"
                     /translation="MGKSQVNLQDIFLNQMRKEKIPVTMYLVNGARLTGTIKGFDNFV
                     ILLKQENQQLVYKHAISTIIPEKPVELLETIDSKRAEQEQPAQA"
     gene            438..1328
                     /gene="mtnP"
                     /locus_tag="VFK23_10075"
     CDS             438..1328
                     /gene="mtnP"
                     /locus_tag="VFK23_10075"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HET7319471.1"
                     /translation="MAKRSAKQEKITIGVIGGSGLYDMEGLTQVKTVKVATPFGKPSD
                     DYVIGTLFGRRVAFLPRHGRGHRIMPTDINYRANIFGMKKLGVERIISVSAVGSMKEE
                     IKPGDIVIPDQFYDHTKHRRSTFFGGGIVAHVGMADPVCTDLGNVLADAGAKAGAAMH
                     KGGTYLCMEGPQFSTRAESIIYRTWNADVIGMTNATEAKLAREAELCYSTIALATDYD
                     CWHHSEETVTVEAVLAVMKKNIETSKAMIREAVRMLPAARSCSCGDALRNTIMTPEKL
                     APAKTKRDLAPIIGKYMKKK"
     gene            1450..2358
                     /locus_tag="VFK23_10080"
     CDS             1450..2358
                     /locus_tag="VFK23_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET7319472.1"
                     /translation="MSLLVVGTVALDSVKTPFGNVDNALGGSATYFSTSASYFTDVRL
                     VAVIGEDFPKEHIQFLRSKAIDTRGLEIQKGETFRWKGEYSYDLNEARTLATDLNVLA
                     TFKPRIADDYKDSEVVFLANVDPEIQLDVLKQVKKPKLVACDTMNYWIASKPDALQRT
                     LREVDLLTINEAEIRQLAEEASLVKAAKRVQAMGPKTIVVKQGSYGAIMFNGHSVFSA
                     PAYPLESVFDPTGAGDTFAGGFMGFLASTMNFTEDSMRKAVIFGSVMASLNVEAFSLD
                     RLRSLDYREIEGRYREFKKLTHFEDI"
     gene            2582..>3539
                     /locus_tag="VFK23_10085"
     CDS             2582..>3539
                     /locus_tag="VFK23_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319473.1"
                     /translation="MFTIDQNITAVGLREKNLVKVFFSMNNHEVATPEMMLEEARSYV
                     MFCSEADGKVSAYIGLHLLQTGRRLFYAHSSNPFSPGETGSVEDEALCFVEGLGALID
                     EIDIARLSAEEKSRWIDGQDIFSPYARLENPPGAPSPRPDETEAVKAESQEKQPSPPA
                     PAEAAQLQNPPAQPEPQEVPAPPVQQAPQIQPAQAAPQPPQAAATTREAPPPPAEEST
                     PAPSASQPDVQAERDDDAQVISSPAEVPEMRAAPKSQPTEKGAGTREAAVPAPERTRP
                     RPAAPAAAGPRNASYANSSTDRRDIRRKTVQDDIGKLLKTPMK"
BASE COUNT          858 a          998 c         1042 g          641 t
ORIGIN      
        1 actgcatgac ggcttcacgg agggctggat gggcaagtcg caggtgaatc tgcaggacat
       61 ttttctgaac cagatgcgca aggaaaagat ccccgtgacc atgtacctgg taaacggtgc
      121 gcggctcact gggacgatca agggcttcga caatttcgtg atccttctga aacaggaaaa
      181 tcagcaactc gtgtacaagc atgccatctc caccatcatc ccggaaaaac ccgtggaact
      241 tctggaaacc atcgactcga aaagggcgga gcaggagcag ccggcgcagg cgtaggacga
      301 ttgaggaatg ccggtccccg attgaatcat tcgaggacaa gatttcggaa tgcggaataa
      361 aagtccacgg cgcaatcaga atcaccgtca catgctgttg aaactgcgtc ctcttttctg
      421 agaaggaatt atccagcatg gcaaagcgca gcgcaaaaca ggaaaagata actatcggcg
      481 tcatcggcgg cagcggcctg tacgatatgg aagggctgac gcaggtaaag actgtgaagg
      541 tcgcaacgcc cttcggcaag ccttctgacg actacgtcat cggaacactc ttcggcaggc
      601 gcgtggcctt tttgccccgt cacggacggg ggcaccgcat catgccgacc gacatcaact
      661 accgagcgaa catcttcggc atgaagaagc tgggcgtcga acggatcatc tcggtctccg
      721 ccgtgggcag catgaaggaa gagatcaagc ccggtgacat cgtgatcccg gaccagttct
      781 atgaccatac aaagcaccgc aggagcactt ttttcggagg cggcatcgtg gcccatgtgg
      841 gcatggcgga cccggtctgc accgacctcg ggaacgtcct ggccgatgca ggagcgaagg
      901 cgggggcagc gatgcacaaa ggcggcacct acctctgcat ggaagggccg cagttttcaa
      961 cgcgcgccga gtcgattatc taccgcacgt ggaacgccga cgtcatcggg atgacgaacg
     1021 ctaccgaggc caagctggcc cgggaagcgg agctctgcta cagcacgatt gccctggcca
     1081 cggactacga ctgctggcac cacagtgagg agacggtgac cgtagaggcg gtcctggccg
     1141 tgatgaagaa aaacatcgaa acgtccaagg ccatgatccg cgaggccgtc aggatgttgc
     1201 ccgcggcgcg gagctgttcc tgtggcgatg cgctccggaa cacgatcatg acgccggaaa
     1261 agctggcccc ggcgaagacg aagagggacc tcgcgccgat catcgggaaa tacatgaaaa
     1321 agaagtgacg ggattattaa ccacagataa acacggttca gggcgaacat aggccatcag
     1381 acgttacttt tcatcctgtg cgtccgtgtt catccgtggt taaagaaatt tttcgtttgg
     1441 aggatcttag tgagtttgct tgttgtcggt acggttgccc ttgattcggt aaaaacccct
     1501 tttggaaacg tcgataacgc tctgggcggt tcagccacct atttttccac gtcagcaagt
     1561 tactttaccg atgtacggct cgtggccgtg atcggtgaag atttccccaa ggaacacata
     1621 caattcctgc ggagcaaggc catcgacacg cgggggctcg agatacagaa gggcgagacc
     1681 ttccgctgga agggcgagta cagctacgat ttgaacgaag cccgtacgct cgccacggac
     1741 ctgaacgtac tggcgacgtt caaaccgagg atcgcggacg actacaagga ttcggaagtc
     1801 gtttttctcg cgaacgtcga tccggagatc cagctcgacg tcctgaaaca ggtaaaaaaa
     1861 ccgaagctcg tggcctgcga taccatgaac tactggatcg caagcaagcc cgatgcgctc
     1921 caacgcacgc tgagggaagt ggacctcctg accatcaacg aagcggaaat aaggcagctt
     1981 gccgaggaag cgagcctcgt caaggcggcc aagcgcgtac aggccatggg gccgaagacg
     2041 atcgtggtca aacaggggag ctacggtgcg atcatgttca acggccacag cgtgttttcc
     2101 gccccggcct atccgctcga gtcggtgttc gacccgacgg gcgccggaga taccttcgcg
     2161 ggcggcttca tggggttcct ggcgagtacg atgaatttta ccgaagacag catgcggaag
     2221 gccgtcatct tcggcagcgt tatggcctcc ttgaatgtgg aggcgttcag cctcgaccgc
     2281 ttgaggagcc ttgactaccg ggaaattgaa ggcaggtacc gcgagttcaa gaaactcacg
     2341 cactttgagg acatataaac agcccttcgc catcattctg aacggagcat actccttcga
     2401 cgctgtcact ctgagcgaag cgaagagtcc aaattcttag cgtctcagga taaactccgt
     2461 gaagaatctg gctccgagca ggctccatga atggcggccg gcagcggcat gaatgtccag
     2521 attcttcgct gcgctcagaa tgacaaactg gctgactcgg catttttggt ttttttatat
     2581 catgttcacc atcgatcaaa acataacggc cgtcgggctc agggagaaaa acctcgtgaa
     2641 ggtctttttt tccatgaaca accacgaggt cgcaacacct gaaatgatgc ttgaggaggc
     2701 ccgcagttac gtcatgtttt gcagcgaggc cgacggaaaa gtgtccgcat acatcggact
     2761 ccatcttctc cagacgggtc gcagactttt ttacgcccac tcttccaatc ctttttcccc
     2821 gggagagacg ggctcagttg aagatgaggc gctgtgtttt gtggaagggc tcggagcgct
     2881 catcgatgag attgacatcg ccagactgtc cgcggaggaa aagagccgct ggatcgatgg
     2941 ccaggacatt ttctcgcctt atgcccgtct tgagaatcct cccggagcgc cgtcaccgcg
     3001 accggacgag acagaggccg tgaaggcgga atcgcaggag aaacaaccgt cgcccccagc
     3061 gcccgcagag gcggcgcaac tgcagaatcc tcccgcgcag cccgagccgc aggaagtacc
     3121 ggcgccgccc gtgcagcagg cgccgcagat acagcctgcc caggcggcac cccagcctcc
     3181 tcaagcagcc gcaacaacgc gcgaggcgcc gcctccgccg gcggaggagt caacgccggc
     3241 gccatccgcg tcacagccgg atgttcaggc ggaaagggat gatgacgcgc aggtgatttc
     3301 gtctcccgcc gaggtccccg aaatgagggc cgcgccgaag tcacagccga ctgaaaaggg
     3361 ggcgggcacc cgggaggccg cagtccctgc tcccgaaaga acaaggccga ggcccgcagc
     3421 ccctgcagca gcgggtccca ggaacgcttc ctatgccaac tcctcaacag acaggcggga
     3481 catcaggaga aaaacggtac aggatgatat cggcaaactg ctgaaaacgc cgatgaaaa
//
LOCUS       DASSLD010000557         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1218101, whole genome shotgun sequence.
ACCESSION   DASSLD010000557 DASSLD010000000
VERSION     DASSLD010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218101"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1011)
                     /locus_tag="VFK23_10090"
     CDS             complement(148..1011)
                     /locus_tag="VFK23_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7319474.1"
                     /translation="MRNKTTLAILLAVILAVSLSYAAQDQQIQQKLAALANIQGKFTF
                     IVIGDNRSGDETYKKLVSLIAERKPDFVVNTGDMINKPGSTQEWARFWELSRPITAPY
                     FLAVGNHDVHQKVPRSEKTYQDQVDLPGNELYYSFDAGNSLFVVLDSFIAGQEKMIAG
                     EQLKWLEALLAGSTRKHKFIFLHHPLYTDLNKGHHAHESLDTYPDSRDALEALFAKWK
                     VDAVFAGHEHYYQRRTVDGVLHIIAGGGGAPLYDREEDGGFYHYVRVTVDGDRVSGEV
                     IDINGKVRDRF"
     gene            complement(1033..>2052)
                     /locus_tag="VFK23_10095"
     CDS             complement(1033..>2052)
                     /locus_tag="VFK23_10095"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET7319475.1"
                     /translation="AARAGGFTSVCCMPNTSPVNDNRSVTEFIISQAAKAAARVYPIG
                     AITKGSRGEELAEMGELHDAGCVAVSDDGKPVMNAAVMRRALEYSRIFGMLVISHCED
                     ALLSDKGVMNEGAVSTELGLRGIPRAAEDVMTARDIALAELTGGRLHIAHVSTEGSVR
                     LVRDAKARGVRVTAETCPHYFSLTEDAVRGYNTMAKMNPPLRTAGDVAAIKEGLRDGT
                     IDAIATDHAPHALDEKSGEFDYAPFGVVGLETALGLTFKLVEEGVLSLSGAIGKLSAN
                     PASILGMDKGTLAEGSDADITVIDPDVEWTVDASQFKSKSKNTPFQGWKLKGRAVRTI
                     VGGIV"
BASE COUNT          367 a          669 c          562 g          454 t
ORIGIN      
        1 gaggtctccc cctcggcccc gaaggaatgc ccttcgaaca ccagcccgtc agccaatgca
       61 agaatcgctt ttttcatttc tttgcctctt tttcatttcg cattccgcac tccgcatcag
      121 cgtgacccgt ttgatcggcc atccgcatta gaacctgtcc cttaccttcc cgtttatatc
      181 gatgacttcc ccgctcaccc ggtccccgtc taccgtcacc cggacgtagt ggtagaaccc
      241 tccgtcttcc tcgcggtcgt acagcggggc gcctccgcct ccggcgatga tgtgcaagac
      301 gccgtcaacc gtccttctct gataatagtg ctcatgcccc gcgaacaccg catccacctt
      361 ccacttcgca aagagcgcct ccagcgcgtc cctgctgtcc ggatacgtgt cgaggctttc
      421 gtgagcatga tgccccttgt tgaggtccgt atagagagga tgatggagga atatgaactt
      481 gtgcttccgc gtcgagcccg cgagcagggc ctcgagccac ttcaactgtt cgccggcaat
      541 catcttctcc tggccggcga taaacgaatc aagcacgacg aacagcgaat tgcccgcatc
      601 gaaggagtaa tagagttcgt ttcccggcag gtccacctgg tcctggtacg tcttttcgct
      661 tcgcgggacc ttctgatgga cgtcgtggtt cccgacggcg agaaaataag gcgcggttat
      721 cggcctggac agttcccaga accgcgccca ttcctgcgtg ctgcctggtt tgttgatcat
      781 gtcgccggtg ttgacgacga agtccggctt tctctccgcg atcagggaaa caagcttctt
      841 gtaggtctcg tctcccgacc ggttgtcgcc gatgacgatg aaggtgaact taccctggat
      901 atttgcaagc gccgcgagct tctgctggat ctgctggtcc tgtgcggcat acgacaaaga
      961 aacagcaagg ataaccgcta agagaatggc gagtgttgtt ttattccgca ttccgaattc
     1021 cgcattccgc aattacacta ttcccccgac aatcgtccgt accgcccttc ctttgagctt
     1081 ccaaccctgg aacggcgtgt tcttcgactt tgatttgaat tgtgacgcat ccacggtcca
     1141 ctccacatcc ggatcgatga ccgtaatgtc cgcatccgag ccttcggcga gcgtgccctt
     1201 gtccatgccg aggatcgacg ccggattcgc gctcagtttc ccgatcgcgc cggaaagcga
     1261 caggacacct tcttccacga gcttgaacgt gagtcccaga gcggtttcga gtccgaccac
     1321 gccgaagggc gcgtagtcga actcgccgga tttttcgtcg agggcatggg gcgcatggtc
     1381 cgtggcgatc gcatcaatgg taccatcccg gaggccctcc ttgatcgcgg cgacatcccc
     1441 cgcggtcctg agcggcgggt tcattttcgc catggtattg taaccccgca ccgcatcctc
     1501 ggtcaaagaa aagtagtggg ggcaggtctc cgccgtgacc cggaccccgc gggccttcgc
     1561 atcccggacg agcctgacgg agccttccgt gctcacgtgc gcgatgtgca gtcttccccc
     1621 cgtcagttcg gcaagggcga tgtcccgcgc cgtcatcacg tcctccgcgg cccggggaat
     1681 cccccgaagc ccgagttccg tcgagacggc gccttcgttc atcacgcctt tgtccgaaag
     1741 aagcgcgtcc tcgcagtgcg agatgacgag catgccgaat attctcgagt actccaatgc
     1801 cctgcgcatg acggcggcgt tcatcaccgg ttttccgtcg tccgagaccg cgacgcatcc
     1861 cgcgtcgtga agctcgccca tctcggccag ttcctcgccg cgggagccct tggtgatggc
     1921 gccgatagga tagacccgcg ccgcggcctt cgcggcctgg gaaataatga actcggtgac
     1981 cgaccggttg tcgtttaccg gactggtgtt cggcatgcag cagaccgagg tgaagccgcc
     2041 ggccctggcc gc
//
LOCUS       DASSLD010000558         2918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1609507, whole genome shotgun sequence.
ACCESSION   DASSLD010000558 DASSLD010000000
VERSION     DASSLD010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609507"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..387)
                     /locus_tag="VFK23_10100"
     CDS             complement(64..387)
                     /locus_tag="VFK23_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET7319476.1"
                     /translation="MKKVLVLSIVLAALMMPFSTRAEKVGGGDLTFTPKNAQPVVFSH
                     EKHVSDKGLKCTACHYQIFQMAQGSYKMDMSKITKGEFCGKCHNGKKAFDVTDSKNCS
                     RCHHS"
     gene            530..1324
                     /locus_tag="VFK23_10105"
     CDS             530..1324
                     /locus_tag="VFK23_10105"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET7319477.1"
                     /translation="MSAVSLKRGERMLSEATLSKIGNMPAELARPVRLILFTSDMACE
                     ACPDMRELALAIKKHTSKIALESYDIVMDRDKSEQYGVKHAPALVVQGTSGQAVTFYG
                     LIEDMVLDVLLSAIRSVSDSRVWFPDNIRHTLKHLTRDVSIRVFVESDCIQCRPVAET
                     AVGLGLESGLVSASVIVASSFPDLIKRYGIKTLPKTIFGENLHMDGHVTESEFLEMIF
                     QSEGVKAGPEKKCLICGTSSPDIICQNCKTRIQAEAVDHKIKSEKR"
     gene            1386..2396
                     /gene="amrS"
                     /locus_tag="VFK23_10110"
     CDS             1386..2396
                     /gene="amrS"
                     /locus_tag="VFK23_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HET7319478.1"
                     /translation="MKTAMFQESREEGKVKCSLCCHRCLILPGKRGICAVRENRDGVL
                     YSLVYDKVIARNIDPIEKKPLFHFQPGSRSFSIATPGCNFRCKHCQNADISQLPHDRG
                     GMILGEDVSPAVLVQAARRLGCTSISYTYTEPTIYFELAYDTARMAAAAGLKNVFVTN
                     GYITPEALEVIRPYLHAANIDLKGFRDDFYREICGARLQHVLDAIRLYKKYGIWIEIT
                     TLVIPGHNDSEEELTGIARFIKSVGEDVPWHVSRFHPTYQLTDQPPTPVETLARARRI
                     GLDAGLRYVYEGNVPGTGEDTQCWNCKEILIKRVGFGVTANAVEKGKCRKCGAVIDGI
                     WE"
BASE COUNT          679 a          813 c          823 g          603 t
ORIGIN      
        1 aaaaaccgaa aaggaagccg tcccgccttt cccttgctta ggcggctcgc cgccgttcct
       61 tcttcacgag tgatggcagc gggaacagtt tttcgaatct gtgacgtcga aggccttttt
      121 gccgttgtgg cattttccgc agaactcgcc tttggttatc ttgctcatgt ccatcttgta
      181 cgagccctga gccatctgga agatctggta gtggcacgcg gtgcacttga gccccttgtc
      241 actcacgtgc ttttcatggc tgaacacgac aggctgtgcg ttcttcggcg tgaaggtcaa
      301 gtcgccgccg ccgaccttct cggcacgggt actgaagggc atcatgagag cggccagcac
      361 gatcgagagg accaaaacct ttttcatctg tttccccttt tccgcaatat gcctgcgcag
      421 gttatgtgcg atcaacggcc attatagcaa gccggggaag gttgttcaag tcttttttct
      481 tggtattcat gccttcccgt tgcccgcggc tgaatctgat ataattgtca tgtcggcagt
      541 atctctcaag agaggtgaac gcatgctttc agaggcgacg ttatcgaaaa tcgggaatat
      601 gcctgcggag ctggcgaggc cggtcaggct cattctgttc acgtcggata tggcatgcga
      661 ggcatgcccc gacatgcggg aactcgcact cgcaataaaa aaacatacca gcaagatcgc
      721 cctggaatcc tatgatatcg tcatggaccg ggacaaatcg gaacagtacg gcgtcaagca
      781 tgcgcccgct ctcgtcgttc agggaacaag cgggcaggca gtcacgttct acggactgat
      841 cgaggacatg gtgctggacg tcctgctgtc cgcgatccgg tccgtctctg actcccgggt
      901 gtggttcccg gataacatca ggcacaccct gaagcacctc acgagagacg tctccatacg
      961 cgtctttgtt gaaagcgatt gcatccaatg cagaccggtg gccgaaaccg cagtcgggct
     1021 cggacttgaa agcggcctcg tttccgcgag cgtcatcgtt gcgagctcct tccccgatct
     1081 gataaagcgc tacggcataa aaacgctgcc caagacgatc ttcggagaga atttgcacat
     1141 ggacggccac gtgacggaaa gcgaattcct cgaaatgatc tttcagtccg aaggggtgaa
     1201 ggcggggccg gagaaaaagt gcctcatctg cgggacctct tcacccgata tcatctgcca
     1261 aaactgcaaa accaggatcc aggcggaggc agtggaccac aagataaaga gcgaaaaaag
     1321 atgaaatcgc agaaaatctt tcggtaagat agcgcatccc ggatcgattg ccgaggtatc
     1381 tgcccgtgaa aacagcgatg tttcaggagt cgagagagga ggggaaggta aagtgcagcc
     1441 tctgctgcca ccgctgtctc atcctgcccg ggaaacgcgg catctgcgcg gtccgcgaga
     1501 accgggacgg cgtgctctac agcctcgtgt acgataaggt cattgcgcga aacatagacc
     1561 ccatcgagaa aaaacccctc tttcattttc aacccggcag ccgttctttt tccatagcga
     1621 cgccgggctg caatttccgc tgcaagcact gccagaacgc agacatctcg cagctccccc
     1681 atgaccgggg aggcatgatc ctgggcgaag acgtttctcc cgccgtcctg gtccaggccg
     1741 cccgcaggct gggctgcacc agcatttcct acacctacac ggaaccgacg atctatttcg
     1801 aactggccta tgatacggcg cgcatggcag cggctgcggg actcaagaac gtgtttgtga
     1861 cgaacggcta cataacgccc gaggcgctgg aggtcatccg gccttatctt catgctgcga
     1921 acatcgacct caagggcttt cgcgacgatt tctaccggga gatctgcggc gcccggctcc
     1981 agcacgtgct cgacgcaata cggctctaca aaaaatacgg catatggatc gagatcacga
     2041 cgctcgttat ccccggccac aacgactcag aagaggaact cacggggatt gcccggttca
     2101 tcaaatcagt gggagaggac gtgccctggc acgtgtcgag gtttcacccc acctatcaat
     2161 tgacggacca gccgcctacg cctgtggaga cgctggcgcg ggcccggcgc atcggcctcg
     2221 atgcggggct ccggtacgtg tacgagggca atgtgccggg aacgggggaa gacacgcagt
     2281 gctggaactg caaggaaatc ctgatcaaga gagtggggtt cggcgtcacc gcgaatgccg
     2341 tcgaaaaggg gaagtgccgc aagtgcggag cagtgattga cggtatctgg gaatgaagcc
     2401 ggaataagca agggttcgaa tttacaaact caacaggagg gaccatgcga tcgatcatca
     2461 ttcttgccgt actcggaacg ttgctgcttc tgcccggtgt gagcttcgca gatgcgaacg
     2521 ctgaatgcca atcaaactgt atcaacgaga aagcgacaag agattccaac tgcccgccgc
     2581 ccgtagaaga ccccaccaat tcggaacgtg ctcattgcct gaatgatagc caggacgaat
     2641 acagcaattg tgtcaagaat tgcccgccgc ccgaaccgat agaggcgccc acggggactt
     2701 aagctgcatc gcttggtttg gcttaacgcg cagagagaga aaggagtggt ggtgaaacgg
     2761 ctatttttga tcatggcgct cgctgcaacg attatttccg caagcgggtg catctatact
     2821 cacgtcacga tgccactcag cacggagctg aacaaaaccg accttgggca taagcaggga
     2881 gagtcgtcca tgtactccgt tctctggctc tttgcatg
//
LOCUS       DASSLD010000559         4933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_952717,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000559 DASSLD010000000
VERSION     DASSLD010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952717"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..951
                     /locus_tag="VFK23_10115"
     CDS             190..951
                     /locus_tag="VFK23_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET7319479.1"
                     /translation="MNRFFSVKTLYAVVVAALIVPAAVFASPEKAVQKPAKVKAAEPE
                     AAESSTLSGKVTETMNSGGYTYVLLEKKGKKIWVAIPQTTVKVGQKLDLQPGQEMVNF
                     HSQSLNRTFENIIFSPGAAQPQGASASPADHEGSKKAAGSAEKGIKVEKATGPNAYTV
                     ADIFAKRAELGQKTAVVRAKVVKVSAGIMGSNWIHLQDGTGDAGKKTHDLVVTTQELP
                     VVGDIVTVSGTVAVDKDFGAGYKYSVLIEKASIQR"
     gene            1121..2908
                     /locus_tag="VFK23_10120"
     CDS             1121..2908
                     /locus_tag="VFK23_10120"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HET7319480.1"
                     /translation="MGNGPGNEKLNRWVSEVADLCRPDSIYWCDGSKAEYDRLMAEMQ
                     ERGMAITLRKRPNSFLFRTDASDVARVEDRTFISTTSHDEAGPTNNWVHPDNLKETMR
                     ALYRGCMKGRTLYVIPFSMGPIGSPISKIGVELTDSPYVVCNMHIMTRVGAKVLETLG
                     RDGEFIPCLHSIGAPLSPGQKDSLWPCAPIEKKYISHFPEENLIWSYGSGYGGNALLG
                     KKCLALRIASNMARKEGWMAEHMLILRLTSPEGKRYHIAAAFPSACGKTNLAMMRPTI
                     RGWKCECIGDDIAWMKIRGDGRLHAINPESGFFGVAPGTSYDTNPVAMDTLRENVIFT
                     NCALTDDGDVWWEGMTEETPAHVVDWKGRDWPPESREDAAHPNARFTAPARQCPVICP
                     DWENPEGVPIDIFIFGGRRSTVVPLVTEAYSWDHGVFMGATAASETTSANIGAVGNLR
                     RDPFAMKPFCGYHMGDYFHHWFAMGDRLGPKAPRIFYVNWFRKSAEGRFLWPGFSDNS
                     RVLKWMCERVDGKIGAKETPIGLMPKEGDLDLAGLNMSPENMKALMNVDLAAWKAEVP
                     DIEQHFALFGNRLPERLKKQLQEFRKRLR"
     gene            2924..4669
                     /locus_tag="VFK23_10125"
     CDS             2924..4669
                     /locus_tag="VFK23_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319481.1"
                     /translation="MLVQKTSHQKALVNVLGVVYLHSKTEDGGDLYLTRHAESHQQHL
                     EVENWYEESWFTQHRVRLLGTSSVYRVPTKPVDGTSLDLVVKHNRVGEDVPLNTHTLQ
                     EFTSSEFNSPWEEFSLVMEMGDKHVGQRLQWIRVQRPLAIYVPPQRMQAWQSGRSRAK
                     INRIRARHPGVDLDILKQYKLIYEWIRGKNLTEIFEHIKIELPDIVHHLQAMQKMALE
                     DLRKKEYVMADMKPEHVIIDEEDCLRIERAGENGGAETARKQIDMVYKLLTGGKYSVI
                     DYELLFRTPEHEDRIKASRRHSYLDDMRDRLEPTPLPSHLSRTEIFKVPYIFGHAEST
                     GGRMWVVGRNARLFDYFLPERWRKTPSLGLSDTNEVFYTVTKDNIHLVWKTSQVGEIP
                     APGKFSPAEIEKIRHYGINSPFEEFAISQDLNQLGIHAVYVRAIYMTGSLKIEVASDS
                     RKYQSHAGIVDIDGNPVLAAEHNYITIRGYYNGPDEWVSRHEETSLLTPVDLARAVRR
                     KIISEEDCLKYLERMKERLNQAGYDGSLLKPDDLLLALDSKGSIMRDKSGEPDVIICD
                     FETLWKVSGAAEKGA"
     gene            complement(4716..>4933)
                     /locus_tag="VFK23_10130"
     CDS             complement(4716..>4933)
                     /locus_tag="VFK23_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020608133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HET7319482.1"
                     /translation="AAATGDFVLIESEVNPVIKALVDHGIMVTAVHSHMLDESPRLFF
                     LHFWGHDEPEKLASGLKAALDKTNAMK"
BASE COUNT         1083 a         1479 c         1515 g          856 t
ORIGIN      
        1 ggcccggagg cgtgcggaaa gagaaaaaga gcttgctaat tttcggcaaa tgccgtaaat
       61 tattttaatc ctgataaaaa attgggcggc catcttcgat ctctgtccct cctgagtaat
      121 cgacatcgac ttgcctgcat gatcgcctca cggatgaatc agatcaatat ttccaaaagg
      181 agcatattca tgaaccgttt ttttagcgtt aaaacactgt acgcagtcgt ggtagcggca
      241 ttgatcgtac cggccgctgt ttttgcgtca ccggagaagg ccgtccaaaa accggcgaag
      301 gtcaaggcgg cggagccgga agcggccgaa tcatcgaccc tgtcgggcaa ggtgaccgaa
      361 acgatgaatt ccgggggcta cacctatgtg ctcctggaga agaagggcaa gaagatctgg
      421 gtggcgattc cgcagacaac ggtcaaggtc ggccagaaac tggatcttca gcccgggcag
      481 gaaatggtca attttcacag ccagtcgctg aacagaacct ttgagaacat aatcttctcg
      541 ccaggggccg cgcagccgca gggggcgtca gcctcacccg cggaccacga gggcagcaag
      601 aaggcggcgg gttccgcgga aaaggggatc aaggtcgaga aggcaacggg gcctaacgcg
      661 tacacggtgg ccgatatatt cgcaaaacga gcggagctcg gccagaagac cgccgtcgtc
      721 cgggccaagg tcgtcaaggt ctctgcgggg atcatgggat cgaactggat ccacctgcag
      781 gacggcacgg gcgacgcggg aaaaaagacg cacgatcttg tagtgacgac gcaggagctt
      841 ccagttgtag gggacatcgt tacggtgagc gggaccgtcg ccgtcgataa ggatttcggg
      901 gcgggttata aatacagcgt ccttatcgag aaggcgagca tccagcgcta aaccggagtc
      961 aaccgtcaac gtcatcatcg gccccgtgct ccgcacgggg ccttttttgt cgtgaagcgc
     1021 ccttttgatg aacgtatcct ctttactttt attttgcaat tgtgcataat aatccttacc
     1081 tttttatctt tacgtccagc atgctcggtg gaggtcgtcc atggggaacg gaccgggaaa
     1141 cgaaaaattg aacaggtggg taagcgaggt cgctgattta tgccgcccag actcgatcta
     1201 ctggtgcgac ggttcgaagg cggagtacga ccgcctgatg gccgagatgc aggagcgcgg
     1261 catggcgatc accctccgga aacggcccaa cagtttcctg ttccgcaccg acgccagcga
     1321 cgtggcccgc gtggaggacc gcacgttcat cagcacgacg tcgcatgacg aggcggggcc
     1381 caccaacaac tgggtccacc ccgacaatct caaggaaacc atgcgggcgc tttaccgcgg
     1441 atgcatgaag ggccgcaccc tctacgtcat ccccttctcc atgggtccca tcgggtcccc
     1501 catctccaag atcggcgttg aactgaccga cagcccctac gtggtctgca acatgcacat
     1561 catgacccgg gtcggcgcga aggtgctgga gacgctcggg agggacggtg aattcatccc
     1621 ttgcctgcat tccatcggag cgccgctttc gccggggcag aaggacagcc tctggccctg
     1681 cgccccgatc gagaagaagt acatcagcca cttccccgag gagaacctca tctggtccta
     1741 cggctcaggc tacggcggaa acgccctcct ggggaaaaag tgcctggcgc tcaggatcgc
     1801 ctcgaacatg gcacggaagg aaggctggat ggccgagcac atgctgatcc tgcgtctcac
     1861 gagcccggag ggaaagcgct accatatcgc tgccgcgttt ccgtcggcct gcggcaagac
     1921 gaacctcgcc atgatgcggc ccacgattcg gggctggaag tgcgagtgca tcggcgacga
     1981 tatcgcctgg atgaagatcc ggggcgacgg acgcctccac gccatcaacc ccgaaagcgg
     2041 ctttttcggc gtggcgccgg gcacgtccta cgatacgaac cccgtggcaa tggacacgct
     2101 cagggaaaac gtcatcttca cgaactgcgc cctgaccgac gacggggacg tctggtggga
     2161 agggatgacc gaggagactc cggcccacgt ggtcgactgg aaaggccgcg actggccgcc
     2221 ggaaagccgg gaggacgcgg cccatcccaa cgcgcgcttt accgcgccgg cgcggcagtg
     2281 cccggtcatc tgccccgact gggaaaaccc cgagggagtc cccatcgaca tcttcatttt
     2341 cggcggcaga cgctccacgg tcgtgccgct ggtgaccgag gcctattcct gggaccacgg
     2401 tgttttcatg ggtgcgaccg ctgcctcgga gaccacgtcg gcgaacatcg gcgccgtggg
     2461 aaacctcagg cgcgatccct tcgccatgaa accgttctgc ggctaccata tgggggatta
     2521 cttccatcac tggttcgcca tgggcgaccg gctggggccg aaggcgccga ggatcttcta
     2581 cgtcaactgg ttccggaaga gcgctgaggg caggttcctc tggcccgggt tcagcgacaa
     2641 cagccgcgtg ctcaagtgga tgtgcgagcg ggtggacggc aagatcggcg cgaaggagac
     2701 gccgatcggt ctcatgccga aagaggggga cctcgacctc gccggtttga acatgtcacc
     2761 ggagaacatg aaggcgctca tgaacgttga cctcgccgcg tggaaggcag aggtgcccga
     2821 tatcgagcag cacttcgcgc ttttcggaaa tcggctcccg gagcggctca aaaaacagct
     2881 ccaggagttc aggaaacgac tgaggtagga gcgacggaaa accatgctgg tccagaaaac
     2941 ctcccatcag aaagccctcg tgaacgtgct gggcgtggtc tacctccaca gcaaaacgga
     3001 ggatggggga gacctctacc tcacccggca cgccgagtcc caccagcagc acctcgaggt
     3061 cgagaactgg tacgaggaga gctggttcac ccagcaccgg gttcggcttc tggggaccag
     3121 ctcggtgtac cgcgtcccga ccaagcccgt cgacgggaca agtctcgacc tcgtggtcaa
     3181 gcacaaccgc gtgggggagg atgtgcccct caatacccac accctgcagg agttcacgag
     3241 ttccgagttc aacagcccct gggaggagtt ttccctggtc atggagatgg gggacaagca
     3301 cgtcggccag cggctgcagt ggatcagggt ccagcggccc ctcgcgatct acgtgccgcc
     3361 gcagcggatg caggcgtggc agagcggccg gtcccgggcc aagatcaacc gcatccgcgc
     3421 gcgccatccc ggcgtcgacc tcgacatcct caaacagtac aagctcatct atgaatggat
     3481 ccgcggcaag aacctgaccg agatcttcga gcacatcaaa atcgagctcc ccgacatcgt
     3541 gcatcacctg caggccatgc agaagatggc actcgaggac ctgcggaaaa aagagtacgt
     3601 catggcggac atgaagcccg agcacgtgat catcgacgag gaggactgcc tccggatcga
     3661 gcgggccggg gagaacgggg gcgccgagac ggcacggaag cagatcgaca tggtctacaa
     3721 gctcctcact gggggaaagt actcggtgat cgactatgaa ctgctcttcc ggacgcccga
     3781 gcatgaggac cggatcaagg cgtcacggcg ccattcctac ctggacgaca tgcgggaccg
     3841 gctcgagccc acgccgctgc cctcgcacct gagccgcacg gagatcttca aggtccccta
     3901 catcttcggc cacgcggaga gcaccggcgg caggatgtgg gtcgtgggac gaaatgcccg
     3961 cctgttcgac tacttcctgc ccgagcgctg gcgcaagacc ccgtccctgg gcctttccga
     4021 cacgaacgaa gtgttctaca cggtcacgaa ggacaacatc cacctggtct ggaaaacgtc
     4081 acaggtgggc gagatcccgg cgccgggaaa gttcagcccc gcggaaatcg agaagatcag
     4141 gcactacggg atcaacagcc ccttcgagga gttcgccatc tcccaggacc tgaaccagct
     4201 cgggatccat gccgtgtacg tgcgggcgat ctatatgacc ggcagcctga agatagaagt
     4261 ggcgagcgat tcccggaagt accagtccca tgcggggatc gtggatatcg acggcaatcc
     4321 cgtgcttgcc gctgaacaca actacatcac catccgcggc tactacaacg gtccggacga
     4381 atgggtgtcg cggcacgagg agacgtccct ccttacgccc gtggacctcg cccgtgcagt
     4441 gcgcagaaag atcatcagcg aagaagactg cctaaagtac ctcgaacgga tgaaggagcg
     4501 gctgaaccag gcgggctacg acggctcgct cctcaagccg gacgacctcc tgcttgccct
     4561 ggactcgaag ggctcgataa tgcgggacaa gtcgggagag ccggacgtca tcatctgcga
     4621 cttcgagacc ctctggaagg tgtccggcgc ggcggaaaaa ggcgcctgag gccgtcttcc
     4681 ggtccctccc gttactaacc cgcccttcgg tcccctcact tcatggcgtt ggtcttgtcc
     4741 agtgccgctt ttagaccgct ggcgagtttc tccggttcgt catgacccca gaaatgaagg
     4801 aagaagagac gcggtgactc gtcgagcatg tggctgtgca cggcggtgac catgatgcca
     4861 tggtcaacca gcgctttgat taccgggttt acttcgctct caatcaaaac gaaatctccg
     4921 gtggctgcgg cct
//
LOCUS       DASSLD010000560         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_175571,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000560 DASSLD010000000
VERSION     DASSLD010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175571"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..3080)
                     /locus_tag="VFK23_10135"
     CDS             complement(21..3080)
                     /locus_tag="VFK23_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7319483.1"
                     /translation="MRLPEIAIKRPVFVTMIGIALIVFGVVAFPRLALDLFPKVDFPV
                     VNITTKLVGASPEVMEVDVTNTIEEAVNTISGVKAITSRSMEEYSIVTVEFYLERNLE
                     QAAQDVRDKVAAVRNQLPRDTEPPVIEKISPEDQPIVWIAVWGDRSIRDLTHYADKIL
                     KRDIEKIPGVGSVTISGGRARQVRVWIDGRKMNALSLTASDIKDALGREHREVPGGRV
                     ENIRTEYIVKTKGEYETPESFNDLVIAYRHGRLIKLRDIGKAEDGLEDERSITRFNGK
                     TAVGLAVKRQSGENTVAVAERIKAAVAAVKPPQGMKLDITFDQSRFIRRSIEDVQMSL
                     WLGAGLAVLIIFVFLRSVRSTLISAVALPTSVIATFAFIQAFGFTLNNMTMLGLSLSI
                     GILIDDAIVVLENIYRRMEEGEPPMQAALEGSSEIGLAVMATTFSIVAVFVPVAFMKG
                     IVGKFFFEFGITVTVAVLVSLFVSLTLTPMMTSRFLSYRQKHGKVYLFLEKGFDKMYD
                     VYRPLLALALRNRWKVIVIALLSIVAGLALFAKLGKEFLPTEDQGRFMVRLETPIDCS
                     LQRADSMMNKVDEQLRTRPEVASTFYVTSDSELTQAITKGRIYVNMKERKDRTVAQID
                     SMKQVRDSLAAESDLKSSVELVAMVGGGFRSVPIQLMVQGRDLDDINKRTIAIKDEFA
                     KIPGIVDVDTSIETGKPEVRVRIAREKAANFGVSASSIGGTVNTMIGGEIVGKYKDDK
                     EGERYDIVARLFPTDRSHPDDINSLWVRSTSGELVCLRDVTAIQTDTGPTSIMHYNRQ
                     RAATLFAGTDKAKPMAEAMTELNRIVDKNISPEISTRYVGMADAMLDSFKNIAFALMI
                     AVIMVYMILASQFESFVHPFTIKFSLPVSLIGAMGLLFLTGERVQIFSLIGVIMLMGL
                     VTKNAILLVDYTITLRKRGMNRTEALLKAGPVRLRPIIMTTAAMVFGMLPTALKIGEG
                     AESRAPMAITVIGGLITSTLLTLVVIPVVYTLVDDLENFFKRRKAKT"
BASE COUNT          606 a          978 c          917 g          682 t
ORIGIN      
        1 ctgtgtccat ccgtggttga ttacgtcttt gcctttctcc ttttaaagaa attttccagg
       61 tcgtcgacca gcgtgtatac gacagggatg acgacgagcg tcaacagcgt cgaagtgatc
      121 aggccgccga tcacggtgat ggccatgggc gcacgcgatt cggcgccctc gccgatcttc
      181 agggccgtcg gaagcatgcc gaagaccatg gccgcagttg tcatgatgat cggccggagc
      241 cggacagggc cggccttgag gagcgcctcc gtgcggttca taccccgctt tctgagcgtg
      301 atcgtgtaat cgacgagcag gatcgcattc ttggtcacga ggcccatgag catgatcacg
      361 ccgatcaagc tgaatatctg cacccgctcg ccggtcagga acaggagtcc catggcgccg
      421 atgaggctga cggggagcga gaacttgatc gtgaacggat ggacgaagct ttcgaactgc
      481 gacgccagga tcatgtacac catgatgacg gcgatcatca gggcgaaggc gatgttcttg
      541 aaagaatcca gcatggcgtc cgccatgccg acataccgcg tggaaatttc cggcgatatg
      601 ttcttgtcga cgatccggtt cagttcggtc atggcctcgg ccatgggctt cgccttgtcc
      661 gtccccgcga acagggtggc ggccctctgc cggttatagt gcatgatgct ggtgggcccc
      721 gtgtccgtct ggatcgcggt cacgtcccgg agacacacca actctcccga cgtcgagcgg
      781 acccagagcg aattgatatc gtccgggtgg ctccgatcgg tcgggaaaag ccgcgccacg
      841 atatcgtacc gctccccttc cttatcatcc ttgtacttgc cgacgatctc tccgccgatc
      901 atcgtgttga ccgttccccc gatcgaggag gcgctcaccc cgaaattcgc ggctttttcc
      961 cgggcgatcc ggaccctcac ttcgggcttt ccggtctcga ttgaagtatc gacgtcgact
     1021 attccgggga tcttcgcaaa ttcgtccttg atggcgatgg tccgcttgtt gatgtcgtcg
     1081 aggtcccttc cctgaaccat gagctggatg ggcacggacc tgaatcctcc gcccaccatg
     1141 gccacgagtt cgaccgacga cttcaggtca gactcggctg cgagggaatc gcggacctgc
     1201 ttcatggaat cgatctgcgc gacggtgcgg tcctttcgtt ccttcatgtt gacgtagatc
     1261 ctgcctttgg tgatcgcctg cgtcagctcg gagtcgctcg tcacgtagaa cgtgctcgcc
     1321 acttccggcc tcgttctcag ctgttcgtcg accttgttca tcatcgaatc tgcccgctgg
     1381 agagagcagt caatcggggt ttcaagccgc accatgaatc ttccctggtc ttccgtcgga
     1441 aggaattcct tgccgagttt ggcaaagagg gctagacccg cgacgatgct caacagcgcg
     1501 atcacgatca ccttccaccg gtttctgagc gccagggcaa ggagcggccg gtagacgtca
     1561 tacatcttgt cgaaaccctt ttccagaaac agatagacct taccgtgttt ctgccggtag
     1621 ctcaaaaacc gggaggtcat catgggcgtg agcgtgagcg atacgaacag ggagacgagc
     1681 acggcgacgg tcaccgtgat gccgaactcg aaaaaaaact ttcccacgat gcccttcata
     1741 aacgccactg gaacgaagac ggccacgatg gaaaatgtcg tcgccatgac ggcaaggccg
     1801 atctccgagg acccttccag ggccgcctgc atcggcggct ccccttcctc catcctgcgg
     1861 tagatgtttt ccaggaccac gatggcgtcg tcaatcagga tgccgatcga cagcgaaagc
     1921 cccagcatgg tcatattgtt caaggtgaac ccgaaagcct gaatgaaggc aaacgtcgcg
     1981 atcaccgagg tcggcagggc gacggcgctg atcagggtgc tccgcacgct cctgaggaac
     2041 acgaagatga tcaggaccgc cagacccgcg cccagccaga gcgacatctg cacgtcctct
     2101 atggaacggc ggatgaaccg ggactggtcg aaggtgatat cgagcttcat cccctgggga
     2161 ggcttgacgg cagcgacggc cgccttgatc cgctccgcca ccgccaccgt gttctcaccg
     2221 gactggcgtt ttaccgcaag cccgacggcc gttttgccgt tgaacctcgt gatggaccgt
     2281 tcgtcctcga ggccgtcttc cgccttcccg atgtcgcgaa gcttgatgag cctgccgtgg
     2341 cggtacgcga tcaccaggtc gttgaacgac tcgggcgttt cgtactcgcc tttcgtcttc
     2401 acgatatact cggtccggat gttttcgacc cggccgcccg ggacttcacg gtgctcccgc
     2461 cccagggcat ccttgatgtc gctggctgtc agggagaggg cgttcatctt ccggccgtcg
     2521 atccagaccc gcacctggcg ggccctgccg ccgcttatcg tcacggaacc cactccgggg
     2581 atcttctcga tgtcccgctt caggatcttg tcggcgtaat gcgtcaggtc cctgatcgac
     2641 cggtcgcccc agaccgcgat ccagacgatg ggctggtcct cggggctgat cttctcgatc
     2701 accggcggct ccgtatcgcg gggaagctgg ttccgcacgg cggcgacctt gtcccgcacg
     2761 tcctgggcgg cctgctcgag gttccgttcc aggtagaact ccaccgtgac gatggaatac
     2821 tcctccatgc tgcgcgaggt gatggctttg acgccgctga tcgtgttcac ggcctcctcg
     2881 atcgtgttcg tgacgtcgac ttccatgacc tccggggagg cccccacaag cttggtcgtt
     2941 atgttgacta ccggaaaatc gaccttcgga aaaagatcga gcgcgagacg gggaaaggcg
     3001 acgacgccga acacgatcag ggcaatccct atcatggtca cgaataccgg ccgtttaatt
     3061 gcaatttcag gcaaccgcat gattcttgtt cctcacatat ctactcaggt tgtcattccc
     3121 gctgcgaaac atgtcctcga atgctttaat cggggaaggg gaatccattg acgcttccag
     3181 cat
//
LOCUS       DASSLD010000561         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_695980,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000561 DASSLD010000000
VERSION     DASSLD010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695980"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..517)
                     /locus_tag="VFK23_10140"
     CDS             complement(314..517)
                     /locus_tag="VFK23_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319484.1"
                     /translation="MKFSMTCSCGDVMKVEAGTRAEAVKNLKAMMNEKSVAEHMKQKH
                     PGEIIPAIQQVHAMIEQDLKMAA"
     gene            complement(708..1256)
                     /locus_tag="VFK23_10145"
     CDS             complement(708..1256)
                     /locus_tag="VFK23_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HET7319485.1"
                     /translation="MGEGIVEKLKKEFDIAVEWTGVEIHPETPPEGRPLTELYRAADI
                     ERMMKHIREMGTAFGIGFADRPFLSNSRPALQAAEFARERGPFGQFHSALFSAYFSQG
                     LDIGDLEILAQIARDSNLDADAMLDAVRSGTYLPRLHHAQQEAARLGVTGVPTFFIGA
                     GKSVVGAQPLDVFRKVLRSVKT"
     gene            1695..>2407
                     /locus_tag="VFK23_10150"
     CDS             1695..>2407
                     /locus_tag="VFK23_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET7319486.1"
                     /translation="MEKRMLIIGAGIAGLSTGCYAQMNGYKTTIFEMHSIPGGLCTAW
                     KRKGYTFDISMHNFVGSRPGPFYRMWQELGVLEDQEFFYHEEMGRIEGSGKRLDMCPD
                     PKRLEEQMLALSPGDTDLTKEFIRLFSGKGIMNAASLKPAELFGIMDRIRTAAAFLPL
                     IGVFRKYGNMTIQEFAGRFRDPFLRDAVRFFIDSPGWPMVRFPMVALAGMMQAFLASG
                     VPIGGSQRVAYRVADLYKEL"
BASE COUNT          517 a          662 c          672 g          556 t
ORIGIN      
        1 gaggcggcct tcgattgcga gacgggttcg gtggccaagg atgtgaaggt gggtgataag
       61 gtaactgtgc agtataagga agaaggcggc aaaaagacag caacaaaagt ttccccgatg
      121 aagaaggccg gcggatactg atagctactg cagaaaaagg caggcacacc ggtgcctgcc
      181 tttttttatt ggtatgccgg gcatgacgct ctgtacttct gtacaggccc gtcaagtgtg
      241 gaatattagc cgaaaaaaaa acccgcaagc cggagccttc gggttgtatc ctgactgaga
      301 agatgcgcgg accttatgcc gccattttca ggtcctgctc gatcatcgca tgcacttgct
      361 gaattgccgg tattatttcc ccgggatgct tctgtttcat gtgttccgcc accgatttct
      421 cattcatcat cgccttcagg ttcttgacgg cctccgccct tgttccggcc tcgactttca
      481 tgacgtcccc gcaactgcag gtcatgctga atttcattgc ctttacctcc ttcgatatcg
      541 tcgtgctatt ttaactctac taccggaaag ggaaggggtc aaggagcggt gaggcgatgt
      601 agcaccaaag tagtcatata tatacgcggg tatgtccacc cggctattga tccgtcatct
      661 tccgcacttt tttttagaaa gacatccgat ggatccggcc tgtcgtttca ggttttcacg
      721 gagcggagga ccttccggaa cacgtcgagc ggctgggcgc cgacgactga cttcccggcc
      781 ccgatgaaga aggtcggcac accggtcaca ccgagacgtg ctgcctcctg ctgcgcatga
      841 tggagccttg gaaggtaggt cccgcttcgg actgcgtcca gcatggcgtc ggcgtcgagg
      901 tttgagtcgc gtgcgatctg cgcgaggatc tcgaggtccc cgatatcgag tccctgcgaa
      961 aagtaggccg aaaaaagcgc ggaatgaaac tgaccgaacg ggccccgctc gcgggcaaac
     1021 tccgcagcct ggagagcagg ccgggagttc gacaggaagg gccggtcggc gaagccgatc
     1081 ccgaaggccg tgcccatttc gcgaatgtgc ttcatcatgc gctcgatgtc cgccgcgcgg
     1141 taaagctcgg tcaggggcct tccttccggc ggcgtctcgg gatggatctc aacgccggtc
     1201 cattccaccg cgatatcgaa ctctttcttc agtttttcga cgatgccctc gccgatgtag
     1261 cagaagggtc agatgtaatc ggagaagacc tgcatgcgga tggtcatggc ctgccttccg
     1321 acgccttggc ggtcttctgc cgccccagcg tctcacgagg gggttgaaag tcgtgtcgcg
     1381 gtttcatgat cataattaat tctatacaaa cactcctcta aaatcaatct ccggcggaac
     1441 gcctctgctt ttcctctcac cattcctgct caataaaccc ttaatcaggc tgcttccaca
     1501 tcttgtcctc ttctgtttga gttttttcaa ttcagaacgc atccttcagt gcccttcctc
     1561 acaaaacgtg caacacttta tttctaaatg tcggttcact cgtcaatact ccgttcgtca
     1621 gttataatta aactgttcct gttcatcaaa agaaatgacg tcagcgtggc aatgacaaca
     1681 agggaggaat catcatggaa aagagaatgc tcatcatcgg tgcgggaatt gccgggcttt
     1741 cgaccggatg ttatgcccag atgaacggct acaagacgac catcttcgag atgcacagca
     1801 ttccgggggg actatgcact gcctggaagc ggaaggggta caccttcgac atcagcatgc
     1861 acaacttcgt cggcagcagg ccaggaccct tttaccggat gtggcaggaa ctcggcgtgc
     1921 tcgaagacca ggagttcttc taccatgagg agatgggccg catcgaaggc agtggaaagc
     1981 gtctggacat gtgtcccgat cccaagcggt tggaggagca gatgctggcc ctgtcacccg
     2041 gggacacgga cctgacaaaa gagttcatcc ggttgttttc aggaaagggc atcatgaatg
     2101 ccgcttcact gaagcccgct gagctgttcg gcatcatgga caggatcagg acggcagccg
     2161 cctttctgcc gctcatcgga gtgttcagga aatacggtaa tatgacgatc caggagtttg
     2221 cagggcgctt ccgggacccg ttcttacgag acgccgtgcg gttctttata gactcgcccg
     2281 gatggccgat ggtgcgcttc cccatggtcg ccctcgcggg catgatgcag gccttcctcg
     2341 cctcgggcgt gcccatcggc gggtcccaga gggttgccta ccgggtcgcc gatctgtaca
     2401 aagagct
//
LOCUS       DASSLD010000562         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1430291, whole genome shotgun sequence.
ACCESSION   DASSLD010000562 DASSLD010000000
VERSION     DASSLD010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430291"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..1152)
                     /locus_tag="VFK23_10155"
     CDS             complement(193..1152)
                     /locus_tag="VFK23_10155"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET7319487.1"
                     /translation="MRFVKLFVFELLDSAARMWYEYQPDAAPPQRTVMKTENLAIVFV
                     DIAGFTSRTSAQTREENTRLLRRFDDLVRPLVRAYNGRVIKTMGDAYLLTFRSPTDAL
                     LCSMAVHDRIAESDADVDPAERFTVRSAVNVGEVRIKGGDIFGEAVNIASRIEGKAGA
                     GEIYFSESVFLSMTKSEVPSEEVGYTELKGIPGKVRLYRVPRSGEPGGAPQNQAGGGS
                     AAPVNLPFGGWGLDRVRGKLTAGIPAEAAVTQAHGMLVKSLEATGRTFDRWKEGMRRS
                     RAVQVGTALLVLVAIVFTVWAVKHKPKPKPLTPLQKLQRSLGL"
     gene            1222..>2136
                     /locus_tag="VFK23_10160"
     CDS             1222..>2136
                     /locus_tag="VFK23_10160"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7319488.1"
                     /translation="MNSDSVKNGQPALRDALALTLLIGVFFCFMLGNRPLAVPDEGRY
                     VEIPREMAVTGDYITPRLNGVKYFEKPVLFYWLEAFSIRLFGLDEFTLRLWPAIFAVL
                     GCLAVYLAGARLFGRRSGLLAAAVLATSLLYYGLSRVIILDMPVSVLLTASLLSFLAG
                     VREARGWKRRFFLWGFYFFAALAVLTKGLIGILIPALVVGAWFLLLGEWRLLKTMELP
                     AGLALFLLVAVPWHLLAQRANPEFFDFYIVQEHFLRYLTTMHSRYHPAWYFIPVVLLG
                     LFPWSAFLVQAVKYSLPATWKERHEHRDA"
BASE COUNT          374 a          699 c          609 g          454 t
ORIGIN      
        1 aacagcagca ggacgcagcc gctatagaag gcgtggaccc accgaaggtt caacagcaac
       61 tgcctgccga tcaagggata ctgcatgcct ctctcccccc ccataaacat gacgatgccg
      121 ctctttaccc gttaaaaaaa ccatcgctcc aaagcagctc gggaagcgcc agagaacttg
      181 ttttctaacg gtttacagcc ccagcgaacg ctgcagtttc tgcaacgggg tcaagggttt
      241 cggtttgggc ttgtgcttca cggcccatac cgtgaagacg atggctacca gcacgagcag
      301 ggccgtgccg acctgcaccg ccctcgatct ccgcatcccc tctttccacc ggtcgaaagt
      361 ccggcccgtt gcctcgagtg actttaccag cataccgtgc gcctgggtca cggcggcctc
      421 ggccggaatt cctgccgtga gttttccgcg cacccggtca agcccccagc cgccgaaggg
      481 caggttcacc ggcgcagcgc tgccgccgcc tgcctgattc tgcggagcgc ccccgggctc
      541 gcccgagcgc ggcacacggt agagccggac cttcccggga atacctttga gttccgtgta
      601 gcccacttct tcgctcggca cttcgctctt tgtcatggag agaaacacgg actccgagaa
      661 atagatctct ccggcgcctg ccttgccttc gatgcgcgag gcgatattga ccgcctcacc
      721 gaagatgtcg ccgcctttga tgcgcacttc gccgacgttg acggcggagc ggacggtgaa
      781 acgctcggcc gggtcgacgt ccgcatcgct ctccgcaatc cggtcgtgta cggccatgga
      841 gcacaagagg gcgtcagtcg gggaacggaa tgtcaagagg taggcgtcgc ccatggtttt
      901 gatgaccctg ccgttgtagg cccgcacgag gggccggacc aggtcgtcga accgccggag
      961 caggcgggtg ttctcttccc gggtctgggc cgacgtacgc gaggtaaatc ccgcaatgtc
     1021 cacaaatacg atggcgaggt tctctgtctt catgaccgtc ctttgcggcg gtgccgcatc
     1081 cggctgatat tcgtaccaca tgcgggcggc actgtcaagc agttcaaaca caaaaagttt
     1141 gacaaaccgc atcgctacga tgtaaaatcc ttttctctca tcaagactat gctcccgaaa
     1201 ctagcccacg aaacgatggc aatgaattcc gattccgtca agaacggaca acccgccttg
     1261 cgggatgcgc tcgcgctgac cctcctgatc ggcgtcttct tctgcttcat gctcgggaac
     1321 aggcctcttg cagttcccga cgagggacgc tacgtcgaga tcccgcggga gatggccgta
     1381 acaggcgatt acatcacgcc gcggctgaac ggcgtcaaat acttcgaaaa gcccgtcctc
     1441 ttctactggc tcgaggcctt ctccatcagg ctcttcgggc ttgatgagtt cacgctgcgg
     1501 ctctggcccg cgatctttgc ggtcttgggc tgtctcgcgg tctatctcgc gggcgcgcgc
     1561 ctcttcggcc ggcggagcgg gctccttgcc gccgctgtcc tggcaacgag cctcctgtat
     1621 tacggactga gccgggtgat catcctcgat atgccggttt cggtcctgct tacggcttcg
     1681 ctgctctcct tcctcgcggg cgtacgcgag gcccggggtt ggaagcggag gtttttcctc
     1741 tggggcttct acttcttcgc cgcactcgcg gtcctgacaa aaggcctcat cggcatcctc
     1801 atacccgccc tggtcgtcgg ggcatggttc ctcctgctgg gagaatggcg ccttctcaag
     1861 accatggagc ttcctgcggg acttgcgctg tttctgctgg tcgcggtgcc gtggcacctc
     1921 ctggcccagc gggccaatcc ggagtttttc gatttttaca ttgtccagga gcacttcctc
     1981 cgctacctga ccacaatgca cagcaggtat catccggcct ggtacttcat tcccgtcgtg
     2041 ctcctgggcc tgttcccctg gagcgccttc ctcgtccagg cggtgaagta cagtctgccc
     2101 gccacctgga aggaacggca tgagcaccgc gatgcc
//
LOCUS       DASSLD010000563         3568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_272493,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000563 DASSLD010000000
VERSION     DASSLD010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272493"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VFK23_10165"
     CDS             complement(<1..537)
                     /locus_tag="VFK23_10165"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET7319489.1"
                     /translation="MSVLDCIGNTPLARLDRINPNPKVRLFAKLEGNNPGGSVKDRIA
                     WYMVKAAEAEGRLVNGSTILEATSGNTGIGLAMVGTAKGYKVKLVMPECVSLERRKVL
                     EAFGAELVLSPGNEGTDGAIRLAHKIYDEQRQGTFMPNQFDNPANVRAHYETTGRVVY
                     EQTGGEVNVFVAGMGTTGT"
     gene            complement(810..1982)
                     /locus_tag="VFK23_10170"
     CDS             complement(810..1982)
                     /locus_tag="VFK23_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7319490.1"
                     /translation="MFYLQEQRNIIGMLFPDHDDYHALLESVGNFLEAEILPHAKKAD
                     QEMVFPRRNLEKVAEKGIMAIPFPTAYNGGGLPFPIYLAALEMLARACANTALQVDVQ
                     NMACEGLRLFGSERQKDLYLLQHGLAEGKKLIAFALTEPCCGSDAAALRTTAKQTGDS
                     YVLNGSKTLITNPGEADFVLVFARAEKGISAFIVPKGTPGFNVVADMPKLGFRGNKLS
                     AIQIKDCVVPRHDLLGEEGKGIDYAKQMLNAGRLSIAAMAVGVAQAAYDKALAYSKKR
                     GISGGFLSDFQMTREKLADMTTGINAARLLTYYAGMLKERGEEQVSQISQAKLFASET
                     ALRVCDDAIQIHGGYGYTDGFDVHRHWRDARLLTIGEGTSEVLRLLIARLELKEHE"
     gene            complement(2136..2615)
                     /locus_tag="VFK23_10175"
     CDS             complement(2136..2615)
                     /locus_tag="VFK23_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HET7319491.1"
                     /translation="MNKNAKIPETEDGTILFKTDPIIIKHHYEIDYIHSYAQDSPFFA
                     GLAKKKLLGSRCTACGYTYATPRGHCMECGAKTEWFELPLSGKIHTFTTCFYGSEAFL
                     KETPFTLVMVEFEGVNTLFLSRLIGADPDEVKIGMRVRAKFLRNCKFKPTDVYFVPT"
     gene            complement(2767..>3568)
                     /locus_tag="VFK23_10180"
     CDS             complement(2767..>3568)
                     /locus_tag="VFK23_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720401.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HET7319492.1"
                     /translation="EQMAMVSVKNHRNAVHNPYAQSPARLTVADVRSSPMVSTPLTMR
                     DICQMSEGAAVAVLASEESARKLCHEPVRIKGVGSGTDAMRMADRPHGNVILLPHESE
                     EEYKNLKYPGVHSFRAGRTAAKLAYAMAGISNPTTELDFIELHDAYTSSEIQTYEDLG
                     LCRYGEGGKFVESGAPFLANVDYGLEFKPGRWNKIPVNPSGGLLACGHPVGATGLMQA
                     VFAFWQLQGTIGKHLKDDTLQIRDAKRGLIHSHAGTGTYITVTILEKT"
BASE COUNT          657 a         1036 c         1061 g          814 t
ORIGIN      
        1 cgtgccggtc gtgcccatgc ccgcgacgaa cacgttcacc tcgcccccgg tctgctcgta
       61 gaccaccctg cccgtggttt cgtagtgggc ccggacgttc gcggggttgt cgaactggtt
      121 cggcatgaag gtgccctgcc gttgttcgtc gtagatcttg tgcgccagcc ggatcgctcc
      181 gtcggtccct tcgttgccgg gggagaggac gagttccgcg ccgaaggcct ccagcacctt
      241 tctcctctcc aggctgacgc attcaggcat gacgagcttg accttgtacc ccttcgcggt
      301 gccgaccatg gcaagaccga tcccggtgtt gcccgacgtg gcctcaagga tggtcgagcc
      361 attcacgagt cttccttccg cttcggctgc cttgaccatg taccaggcga tgcggtcctt
      421 caccgacccg ccgggattgt ttccttcaag cttggcaaag agcctgacct tgggattcgg
      481 attgatcctg tccagccgcg cgagcggcgt gttgccgatg caatcgagaa cgctcatgaa
      541 cgcttacccc cggaatgccc agtgacttcc gggctgtagt atacggaatt tgcttcttat
      601 ccgcaatatt tttatcccgc cgccgttctg tcagtatttt ttttcgtcat tcacgagaaa
      661 gtcggaaacc aggactttga aaagctcttg aaatctaacg gaaccccatg tgcggatgac
      721 ggagtgacga atgacgttga aatgagaccg ggcatcatcg ggagaaggga cgggaaaatg
      781 cggatgagga ggtcgttttg gggcaggaac tactcatgct ccttcagctc gaggcgggcg
      841 atgaggagcc gcaggacctc ggaggtccct tcgccgatgg tgagaagccg cgcatctctc
      901 caatgccggt gcacgtcgaa tccgtctgtg tagccgtagc cgccatggat ctggatcgcg
      961 tcatcgcata cccgaagggc cgtctcggag gcgaaaagct tggcctgcga tatctgcgat
     1021 acctgctctt cgccccgttc tttcagcatt ccggcatagt atgtcaagag gcgcgccgca
     1081 ttgatgccgg tcgtcatgtc ggccagcttt tcccgggtca tctgaaaatc cgacaggaac
     1141 ccgcccgata tccccctttt tttactgtag gcgagggcct tgtcataggc cgcctgggcg
     1201 acgcccacgg ccatcgccgc gatggacagc cttcccgcgt tcagcatctg cttggcgtaa
     1261 tcgattccct tgccctcttc tccgaggagg tcgtgccgcg gcacgacgca atccttgatc
     1321 tggatcgccg agagcttgtt ccccctgaac cccagcttcg gcatatcggc cacgacgttg
     1381 aagccgggcg tccccttcgg cacgataaag gcagagatgc ctttctcggc tctggcgaag
     1441 acgaggacga agtcggcttc cccggggttc gttatgaggg tcttgctccc gttcagtaca
     1501 tacgaatcac cagtctgttt tgccgttgtc cggagcgcgg ccgcatcgga gccgcagcag
     1561 ggctcggtca gcgcgaaggc tattaatttt ttcccttccg cgagaccgtg ttgcaggaga
     1621 tacaggtctt tctggcgttc gcttccgaaa agccgcaagc cttcacaggc catgttctgg
     1681 acatcgacct gaagtgctgt gttggcgcag gccctggcca gcatttcgag ggccgcgagg
     1741 tagatgggaa agggaagccc cccgccgttg taggccgtgg ggaaggggat ggccatgatg
     1801 cctttttccg ccactttttc aaggttccgt ctcggaaaca ccatctcctg gtccgccttc
     1861 ttcgcatgcg gcaggatctc cgcctccagg aaatttccta cggactcgag cagggcatgg
     1921 taatcgtcat ggtcgggaaa gagcatcccg atgatgtttc gctgctcctg aaggtagaac
     1981 atgggtacct cgtcatcatc gatattccct ccccttcatg gccgatgaaa cgggccacgc
     2041 tgaggggagg gcgcgagggt ggggatgggt taaatccggt agcaatgaac ccatccccct
     2101 cctagcctcc accttgaagg gggaggaata acatcctacg tcggcacgaa gtagacatct
     2161 gtgggtttga acttgcagtt ccggagaaac ttcgcccgca ctctcatccc gatctttacc
     2221 tcatcaggat ctgcgccgat gagcctcgaa agaaagaggg tgttcacgcc ctcgaactcg
     2281 accatgacca gcgtgaaagg ggtctctttg agaaacgcct cgctcccgta gaagcacgtt
     2341 gtgaaggtgt ggattttgcc ggaaaggggc agctcgaacc actcggtctt tgccccgcat
     2401 tccatgcagt gtccgcgcgg agtcgcatag gtatagccgc atgcggtgca gcggcttccc
     2461 aggagtttct ttttcgcgag gccggcgaag aacggcgagt cctgggcgta tgagtggatg
     2521 tagtcgatct cataatggtg cttgatgatg atcggatcag tcttaaagag gatcgtcccg
     2581 tcctcggttt cggggatctt ggcgtttttg ttcattgctt ttgccctctt gcctcattgc
     2641 tgcaataacc catccccacc ctgaccctcc cctcagcgtg gcccgtttca tcggccatga
     2701 aggggaggga atataaatct tctccccctt gaagggcgag attgcgaggg ggatggattg
     2761 cgtgacctat gttttttcca ggatcgtcac cgtgatatac gtcccggtgc cggcatggct
     2821 gtgaataagc ccgcgcttcg catcgcggat ctggagcgtg tcgtccttca ggtgtttccc
     2881 gatcgttccc tggagctgcc agaaggcgaa gacggcctgc atgaggcccg tggcgcctac
     2941 gggatggccg caggcgagga gccctccgga cgggttcaca ggaattttat tccagcggcc
     3001 cggcttgaat tcgagtccgt aatcgacgtt tgcaaggaac ggcgcgccgg actcgacgaa
     3061 cttcccgcct tcgccgtacc ggcagagacc caggtcttcg tatgtctgga tctcggacga
     3121 ggtgtaagcg tcatgaagct cgatgaagtc aagctccgtt gtcggattgg atatccccgc
     3181 catggcatag gcaagcttgg ccgcggtcct gcctgcgcgg aaggagtgga ctccagggta
     3241 tttgagattt ttgtattcct cttcagactc gtgaggaagg aggatcacgt tgccgtgcgg
     3301 acggtcagcc attctcatgg cgtccgtgcc gctgccgaca cccttgatcc tgaccggctc
     3361 gtggcagagt ttccgggccg actcttcgga cgcgaggacc gcgaccgccg caccctcgga
     3421 catctgacag atatcgcgca tggtaagggg agtggagacc atgggggatg agcgaacgtc
     3481 tgcaaccgtg agccttgccg gactctgagc ataggggtta tgcacggcgt tcctgtggtt
     3541 cttgacggag accatggcca tttgctcc
//
LOCUS       DASSLD010000564         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_670354,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000564 DASSLD010000000
VERSION     DASSLD010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670354"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VFK23_10185"
     CDS             <1..874
                     /locus_tag="VFK23_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HET7319493.1"
                     /translation="VVIRILDKESITAGVNTLRLDSLGFDEDDIRRFRKSIREPYGMV
                     LVTGPTGSGKTTTLYAAISEINTGEDKFITIEDPVEYQLRGVTQIPVNEKKGLTFARG
                     LRSILRHDPDKIMVGEIRDAETGQIAVQSALTGHLVFTTVHANNAFDVLGRFLNMGIE
                     PYNFVSSLNCILAQRLVRILCTRCKKPVKLSRAEMEESAVSFDHHKDHQFYEVGGCKE
                     CNYTGYRGRQAITEFLDLSDHIREMILEKRPSSELRKAAIAEGMTTLRQSALAKVFAG
                     STTFKEINRVTFIE"
     gene            1085..>2044
                     /locus_tag="VFK23_10190"
     CDS             1085..>2044
                     /locus_tag="VFK23_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319494.1"
                     /translation="MKIVQATILSILAALILIVAPSGGPASGQTAPDNSAAVLPAPVN
                     VEKGTAIPYRVLLWTGKPDSVLERALTELIVKQAFEEDGFYYRTSASAADFADQAASG
                     LFNIFVVFEADERPVNLDALRERVSRGASIVIIGPEEQSRLVAGSFGFRFLEGSFRDG
                     RSSLVFSGEAGLDLEGSVPVSGSLLLPQKPGSSPVAVYDDIGKPAVLADAAGSGRTIV
                     MPFSIIRSAREAGITSVYSLLLRAAVRSAAQEVPEQGRILGEELTVTSSVDHLRTQLL
                     VTLPKGARMLGVSPGGTLKDNTILFELTAEQFPQKLQYLYELPG"
BASE COUNT          430 a          632 c          599 g          383 t
ORIGIN      
        1 cgtggtcatc cgcatcctgg acaaggaatc catcaccgca ggcgtgaaca ccctccggct
       61 cgacagtctc ggcttcgacg aggacgacat ccggaggttc cggaagagca tccgcgagcc
      121 ctacggcatg gtcctcgtca cgggccccac cggaagcggc aagaccacga cgctctatgc
      181 cgctatcagc gagatcaaca cgggcgagga caaattcatc accatcgagg accccgtcga
      241 ataccagctc cggggcgtga cgcagatccc tgtgaacgag aagaaggggc tcaccttcgc
      301 gcgcgggctc cgttcgatcc tccggcacga tcccgacaag atcatggtcg gcgaaatccg
      361 cgacgccgaa acgggccaga tcgctgtcca gtccgcgctc accggccacc tggtgttcac
      421 cacggtgcac gccaacaacg ccttcgacgt cctcggccgg ttcctgaaca tgggcatcga
      481 gccgtacaat ttcgtatcgt cgctcaattg catcctcgcc cagcggctcg tcaggatcct
      541 ctgcacgcgg tgcaagaagc cggtcaagct ctcgcgcgca gagatggagg aatcggccgt
      601 cagttttgat caccacaagg accaccagtt ctacgaggtc ggcggctgca aggagtgcaa
      661 ctacaccggt taccggggaa ggcaggccat taccgaattc ctcgacctgt cggaccacat
      721 ccgggagatg atccttgaaa agcgtccctc gtcggaattg aggaaagcag caattgccga
      781 ggggatgacc acccttcgcc agtccgcgct cgccaaggtc tttgccggct cgacaacttt
      841 caaggagatc aaccgggtga cgttcatcga ataaaagaat gcccactaca ctccttgagg
      901 ctttataatt atagagagag aaccatatcc ctgatagaat tgttcgggga tgacataagt
      961 aaatggaaaa agttgtcatt cccgagggtt taaaagcctg ccccagcacg ccttaagccg
     1021 gggggaatag ggttccagca ttgccccgat gatatagtca agtggatgcc cgtttataga
     1081 aattatgaag atcgttcaag cgacaatact gtccatcctg gcggcgttaa tcctcatcgt
     1141 cgccccgtcc ggcggcccgg cgtcgggtca gaccgcgccg gacaattccg ccgcggtcct
     1201 tccggcgccg gtgaacgtgg agaaagggac ggctatcccc tatcgggtcc tgctctggac
     1261 cggaaaaccg gacagtgtgc tggaaagggc gctgaccgag ctcatcgtga agcaggcctt
     1321 cgaggaggac gggttttatt accggacttc cgcttccgcc gcggatttcg cagaccaggc
     1381 ggcgagcggc ctctttaata tcttcgtcgt attcgaggca gatgaacgcc ccgtaaacct
     1441 cgacgctctt cgggaacggg tctcccgggg cgcgagcatc gtcatcatag ggccggaaga
     1501 gcagagccgc ctcgtcgcgg ggtccttcgg gttccgtttt ctggaagggt cttttcgcga
     1561 cggaagatca tcgctcgtgt tctccgggga ggcgggattg gacctggaag gaagcgttcc
     1621 tgtgagcgga tccctccttc tcccgcaaaa accgggttca tctccggttg ccgtctatga
     1681 cgacatcggg aagccggcgg tactggccga cgccgcggga agcggacgca cgatcgtcat
     1741 gcctttttcc atcatccgct cggcacgcga ggccgggatc acatccgtct atagtctgct
     1801 gctccgcgcg gcggtccgga gcgccgcgca ggaggtgccc gaacagggcc ggatcctcgg
     1861 cgaagagctc accgtgacct catccgtcga tcacctgaga acgcagctgc tcgtaacgct
     1921 gccgaaggga gccagaatgc tcggggtttc tcccggcgga acgctcaagg acaacaccat
     1981 cctcttcgag ctcacggccg agcagttccc gcagaagctc cagtatctgt atgagctccc
     2041 cggc
//
LOCUS       DASSLD010000565         2125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_62424,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000565 DASSLD010000000
VERSION     DASSLD010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62424"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1215)
                     /locus_tag="VFK23_10195"
     CDS             complement(19..1215)
                     /locus_tag="VFK23_10195"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HET7319495.1"
                     /translation="MMNLSGLFSAFRSSLLVLPRLRTPREGEERHATWLELFYDLVVV
                     AAVASVAQALSKDHSASGILMFVGLFIPVFWVWAGHTVYATRFDTDDLVYRLLTFLQM
                     FAVVGMAVEVHDASQGDTQAFSLAYLGARIILLLLLARADRHVPDARPFIRTYLKGFG
                     VGAALWALSIPLAAPARYVLWGASLGLELAVPWQVWRSMHPTAAVSPSHIPERLGLFT
                     IIVLGESISAVVRGLGGTHWEAAAIAVSALGFGSGVCVWWIYFLHLERAIGRVRLGSG
                     QPYIYSHFPLLVGIVVMGTGVEHAISESGAGRFREGTFGLLWGGVYLWVLGAWILHHV
                     MHPRKSPAALRYAVLLAVLVAANFASRFLSPLSAMVLLLCCLLFLLVEEERIHWTAAR
                     HGSSQD"
     gene            1330..1875
                     /locus_tag="VFK23_10200"
     CDS             1330..1875
                     /locus_tag="VFK23_10200"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET7319496.1"
                     /translation="MPRYIAFLRAINIGGRVVKMDRLRELFEALPLTNVETFIASGNV
                     IFESSVKNTAGLESRIEKHLADALGYEVTTFLRTVEEVRRIAEYQPFRDEDVNKAGAF
                     VVGFLKKPLDRNAGKVLMGFKSAIDDFHLNEREIYWLCKRRQSQSKFSNAAFEKALGL
                     RSTFRGMNTIRNLAEKLRNNV"
BASE COUNT          522 a          603 c          634 g          366 t
ORIGIN      
        1 gcttttatcg acagcccttt agtcctgact gcttccgtgc cgggctgctg tccagtggat
       61 gcgctcctcc tccacgagca ggaagaggag gcagcagagc aacagtacca tggcggaaag
      121 gggcgagagg aaccggctcg cgaagttcgc cgccacgagg accgcgagta gcacggcata
      181 ccggagcgcc gcgggcgatt tccgggggtg catgacgtgg tgcagaatcc acgccccgag
      241 cacccagaga tagaccccgc cccagaggag gccgaaggtt ccttctcgga atcggcccgc
      301 gccgctttca ctgatggcat gttcgacacc ggttcccatc acgacgatcc cgacaaggag
      361 gggaaagtgg ctgtagatat aaggctgtcc gctccccagc ctgaccctgc cgatcgcccg
      421 ttcgaggtgc agaaaataga tccaccagac gcagacgccg ctgccgaagc cgagagccga
      481 aaccgcgatg gccgctgcct cccaatgcgt tcccccgagg ccgcgcacca cggcgctgat
      541 ggactcgccc aatacaataa tggtaaagag ccctaaccgt tcggggatgt gtgaggggct
      601 caccgcagcc gtgggatgca tgctccgcca gacctgccag ggaacggcaa gctccagacc
      661 caggctcgcc ccccagagga cgtaccgtgc cggcgcggct agcgggatcg agagcgccca
      721 gagcgcggcc cccacgccga accctttgag ataggtgcgg atgaagggac gcgcatccgg
      781 aacgtgcctg tccgcgcggg cgaggagaag aaggaggatg atcctcgccc cgagataggc
      841 gaggctgaag gcctgggtat ctccctgcga cgcatcatgg acttccacgg ccatacccac
      901 gaccgcgaac atctggagga atgtgaggag ccggtagacg aggtcgtcgg tgtcgaatct
      961 cgttgcatag accgtgtggc cggcccagac ccagaaaacg ggaatgaaca ggccgacgaa
     1021 catgaggata cccgaggcag agtggtcctt cgagagcgcc tgggccaccg acgccacggc
     1081 cgctaccacc acgagatcgt aaaagagctc gagccacgtg gcatgcctct cctctccttc
     1141 ccgcggcgtc cgcagtcggg gcaggacgag gagggacgag cgaaacgcgg aaaataggcc
     1201 ggacaggttc atcattgcgg ggttacgcag tcctcagacg tttgcgggaa tagggaaatt
     1261 ctggcacagg catagagtca tgtcaaggaa gactgctgaa gaagagtatc aggaggtgag
     1321 gtaataatca tgccgagata tattgcattt ctccgggcca tcaatatcgg cggccgcgtc
     1381 gtcaagatgg accgcttacg cgaattgttc gaagcgcttc cgttaaccaa cgtggaaacg
     1441 ttcatcgcga gcggaaacgt gattttcgaa agcagcgtaa aaaatacggc aggccttgag
     1501 tcacgaatcg aaaagcatct tgccgatgcg ctcggctacg aagtcacgac atttctccgc
     1561 accgtagaag aggtccggcg gattgcggag taccagcctt tccgggatga ggatgtaaat
     1621 aaagccggag ccttcgtggt aggttttctc aaaaagccac tcgacaggaa cgcgggaaaa
     1681 gtcctcatgg gatttaaaag cgcaatcgac gatttccatc tgaacgagcg ggaaatctac
     1741 tggctgtgca agaggaggca gagtcagtcg aagttcagca acgcagcgtt cgaaaaggcg
     1801 ctcggtcttc gctccacctt ccgggggatg aatacaatca ggaatcttgc agagaaactc
     1861 agaaataatg tttaaccctc gtcctccgta gtaacagccg cgaatctcgc gaatgccgcc
     1921 aatagaatca taaaatcagc gtttattggc gtccgttagc ggttgaacaa catcatactg
     1981 cttccccctt tcttctgaac ccgccttgat caaccgaccg gtgctatact cgtatcataa
     2041 tatgacttgt ccgctgccgg acggtcctgc gagaaacaac aagaggaggc tggcatgaaa
     2101 acgaagaaag aagaatacat cgaca
//
LOCUS       DASSLD010000566         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_891865,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000566 DASSLD010000000
VERSION     DASSLD010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891865"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFK23_10205"
     CDS             <1..495
                     /locus_tag="VFK23_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7319497.1"
                     /translation="SHEGKEIILELISPTDIFGGVAVLRNFPYPANAVAMEETEVIKI
                     SRKNLMRLVDRFPNLMYCIALQLGDRMKSSYDSLKNIALERVEARIAALLLKLAGKVG
                     IETKEGLLIDMRLTKQDVADMVGTSVETSIRTFSKFKKQGFLSDKDGKIVIRDRDGLE
                     ELAS"
     gene            complement(570..983)
                     /locus_tag="VFK23_10210"
     CDS             complement(570..983)
                     /locus_tag="VFK23_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319498.1"
                     /translation="MDRYVKGFIVMSIVYLGVAALLGIVMLGNQNVMALKFVHSHLNM
                     LGWVSMMIYGVGYHILPRFMGRPVYSNKVGEAQFYLGNISLITLLSFYTLNVYNPSDM
                     YRMLTVASGAVQALTIFLFFYNMLMTLMTKVEQPH"
     gene            1157..1369
                     /locus_tag="VFK23_10215"
     CDS             1157..1369
                     /locus_tag="VFK23_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HET7319499.1"
                     /translation="MITEKKKVTKDTIIGDVIKMSPEAKAVIQQYFGNGCFTCAGINM
                     ESISFGSMMHNMDPNKIVDEINALEE"
     gene            1371..1508
                     /locus_tag="VFK23_10220"
     CDS             1371..1508
                     /locus_tag="VFK23_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319500.1"
                     /translation="MAAKELKCFVCGSDEKSKVYLPCWHEGKEKVVCTRCLPMLIHGA
                     H"
     gene            1635..2333
                     /gene="queC"
                     /locus_tag="VFK23_10225"
     CDS             1635..2333
                     /gene="queC"
                     /locus_tag="VFK23_10225"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005021919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HET7319501.1"
                     /translation="MDKQLAIVLVSGGMDSCVTAALANEEHRLAFLHVNYGQRTEARE
                     LKAFKDVADFYHADQRLVVSLEHLKAIGGSALTDDSIPVPVASVSQSSITNQQSTSPV
                     TYVPFRNAHLLSIAVSWAEVIGASKIFIGAVEEDSSGYPDCRETFYRAFNKVVETGTK
                     PETRIEIITPLIHMKKSEIVRKGAELGAPFHLTWSCYQNNEKACGRCESCALRLKGFR
                     KAGISDPIPYESNI"
     gene            2426..>2608
                     /locus_tag="VFK23_10230"
     CDS             2426..>2608
                     /locus_tag="VFK23_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319502.1"
                     /translation="MNLTEFIQSWPRLKKQVKGVKMPWLENLAVRDRDPFKVLISCIL
                     SLRTQDRTTGPASERLF"
BASE COUNT          657 a          729 c          681 g          541 t
ORIGIN      
        1 tctcacgagg ggaaggagat catcctggag ctgatatcgc cgaccgacat tttcggcgga
       61 gtcgcggtgc tgcggaactt cccctacccg gccaacgccg tcgcaatgga agaaaccgag
      121 gtcatcaaga tatcccggaa aaacctcatg cgcctcgtgg accgctttcc gaacctcatg
      181 tactgcatcg cccttcagct cggggaccgt atgaagagtt cctacgactc gctcaagaac
      241 atcgccttgg agcgcgtcga ggcccgcatc gccgcgctgc ttctgaaact cgccggcaag
      301 gtcggcattg agacaaagga gggcctcctc atcgatatga ggctcaccaa gcaggacgtg
      361 gccgacatgg tcggcacgtc cgtcgagacc tcgatccgca ccttcagcaa gttcaagaag
      421 caggggttcc tgtcggacaa ggacggcaag atcgtcatca gggatcggga cgggctggag
      481 gagcttgcgt cttaatattg catagagtta tagcgcatgg gcaaaaggct cgaggcacag
      541 catctatgct ctgcgctttg cgctctgcgt caatgcggct gttcgacctt cgtcatcagg
      601 gtcatcagca tattgtagaa aaagagaaag atggtcaatg cctggaccgc gcccgacgcg
      661 acggtcagca tccggtacat atcgctcggg ttgtagacgt tcagcgtgta gaacgagagc
      721 agggtgatca gcgagatgtt cccgagatag aactgcgcct cgccgacttt gttgctgtac
      781 accggccttc ccatgaaccg cggcaggatg tgatagccga cgccgtagat catcattgaa
      841 acccatccca gcatgttcag gtgggaatgc acgaatttca aggccatcac gttctggttc
      901 cccagcatga cgatgccgag cagtgccgcg acccccagat agacgatgct catcacgata
      961 aaccccttca catatctgtc cataacgata cacctcctgt agttgtatga tgctccaagt
     1021 atagaacatc ccgcgcctgc gcgctatgat gtgcctcata gaacgaagaa aaacgggact
     1081 atgacgcagg tcataacggt tgcggccgga atatgctaga gtagaatcaa caatcacact
     1141 gcataaggag gcaactatga tcacggagaa aaagaaagta acgaaggata ccatcatcgg
     1201 tgatgtcatc aagatgagcc ctgaagccaa ggcggtgatc cagcaatact tcggcaacgg
     1261 ctgcttcacc tgcgcgggca tcaacatgga atccatatcc tttggctcca tgatgcacaa
     1321 catggacccg aacaagatcg ttgacgagat caacgccctg gaggaataag atggcggcca
     1381 aggaacttaa atgcttcgtc tgcgggtccg acgaaaagtc gaaggtatac ctgccttgct
     1441 ggcacgaggg aaaagaaaag gtggtctgca cccggtgcct gcccatgctg atccacggag
     1501 ctcactaata tactttcccc ctcccccggc tgaggtatgc tgtcctcgcc gggggctttc
     1561 cttgttctct atggcaagcg cctgtgcgag aaaattcagt agaatttcca tccccgcact
     1621 ggtaaactgg cttcatggac aaacaacttg caatagttct tgtgagcggg gggatggaca
     1681 gttgcgttac ggccgcactc gcgaatgaag agcaccgcct tgcctttctc cacgtgaact
     1741 acggccagag gaccgaggcc cgggaactca aggcgttcaa ggacgtggcc gatttttacc
     1801 atgcggacca gcgccttgtc gtgagcctcg aacacctcaa ggcaatcggc ggctccgccc
     1861 tgacggatga ttcgatccct gtccccgttg cttcggtttc tcaatcatca atcaccaatc
     1921 agcaatcgac aagccccgtt acctacgtcc ccttccggaa tgcgcacctc ctctccatag
     1981 cagtttcctg ggctgaagtc atcggcgctt cgaagatatt catcggtgca gtggaagaag
     2041 acagctccgg atatcccgat tgccgggaga cgttctaccg ggctttcaac aaagttgtag
     2101 agacgggaac aaagcccgag acgcgcatcg agatcatcac cccgctcatc catatgaaaa
     2161 aatcggagat cgtcaggaag ggagccgagc tcggcgcacc attccatctt acttggagct
     2221 gctaccagaa caatgaaaag gcttgcggca ggtgcgaaag ctgcgctctg cggttaaaag
     2281 gattccggaa ggcgggaatt tccgatccga taccgtatga gtctaatatt tgaaccgcca
     2341 aggcacaaag ttttaagaaa cctaaaacaa agaaatctta cttcacgacc ttcacttctt
     2401 cgcggtaata aaattatcct gttgcatgaa tctgactgaa tttatccaat cctggccccg
     2461 cctgaaaaaa caggtaaagg gcgtgaagat gccctggctc gaaaatctcg cagtcagaga
     2521 ccgcgatcct ttcaaggtcc tcatctcctg cattctgagc ctccggaccc aggacagaac
     2581 aacgggtccg gcctcagaac gccttttc
//
LOCUS       DASSLD010000567         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1009179, whole genome shotgun sequence.
ACCESSION   DASSLD010000567 DASSLD010000000
VERSION     DASSLD010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009179"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFK23_10235"
     CDS             <1..189
                     /locus_tag="VFK23_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319503.1"
                     /translation="PNAECGFRNVEYRLEGFSSSASWFVIPAEAGIHFLSAMKATELR
                     IVPGITEKYNLLPVKALS"
     gene            186..950
                     /gene="larB"
                     /locus_tag="VFK23_10240"
     CDS             186..950
                     /gene="larB"
                     /locus_tag="VFK23_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HET7319504.1"
                     /translation="MNKSDLTALLRDVRAGKTDVETALIRLKHLPFEDVVYAHIDHHR
                     HLRHGMPEVIYCEGKTLDQVVGIVKRMLKAGSDVLATRANETIYKAVKKLDRRAVYHE
                     ASRALVIRHEGKKPTRGVVLVLTAGTSDIPVAEEAAVTAEMLGSAVQTVYDVGVAGIH
                     RVLSKRKILDAARVVVVVAGMDGALPSVVGGLVDKPVIAVPTSVGYGAGFEGLAPLLT
                     MLNSCAAGIAVMNIDNGFGAGVLAHRINLLGENLGV"
     gene            1053..>2128
                     /gene="larC"
                     /locus_tag="VFK23_10245"
     CDS             1053..>2128
                     /gene="larC"
                     /locus_tag="VFK23_10245"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HET7319505.1"
                     /translation="MTLAYFDCFSGISGDMTLGALVDAGVSIDVLRSELAKLNLPGYE
                     LKSEKVKRAGIAATKVHVIIGKKGRESRHLSDITAIINNSPLAAGIKEKSVGIFNRLA
                     EAEAKVHATTPEKVHFHEVGAVDAVVDIVGSVIGLDLLGVTRIRASAVNVGSGTVETS
                     HGLLPVPAPAAAELLKGIPFYQSEARFELATPTGAAIISTLGSSFGPLPEMKVNHIGY
                     GAGDGDFPDRPNVLRLMLGEPAVAYDEDSSILIETNIDDMNPQVYEYIIDKLMKQGAK
                     DAYLTPIIMKKGRPAILLSVLTDTPYVDRIVDAIFRETTSIGVRINEVKRKKLNREIR
                     VVETSYGKIRIKVSKRGDEILTATP"
BASE COUNT          489 a          620 c          607 g          412 t
ORIGIN      
        1 ccgaatgcgg aatgcggatt tcggaatgtg gaatacagac ttgaaggatt cagtagcagc
       61 gcctcttggt ttgtcattcc tgcggaagcg ggaatccatt ttttatctgc aatgaaagcc
      121 actgaattgc ggatcgtgcc cgggattaca gaaaagtata atttattgcc agtgaaagcg
      181 ttatcatgaa caaatcagac ctcaccgcgc ttctccggga cgtccgcgca ggcaagaccg
      241 atgtcgaaac ggctctcatt cggctcaaac acctcccgtt tgaggatgtg gtctacgcgc
      301 acatcgatca ccaccgccac ctgcgccacg gcatgcccga agtcatctac tgcgaaggga
      361 aaacgctcga tcaggtagtc ggcatcgtga aacgcatgct caaggcaggc agtgatgtcc
      421 tcgccacgcg cgcgaatgag acgatctata aagccgtcaa aaagctcgac cgcagggctg
      481 tttatcatga ggcgtcccgc gccctcgtga tccggcatga ggggaagaaa cccaccaggg
      541 gggtcgtcct ggtgctgacg gcggggacat cggatattcc cgtggccgaa gaagccgcag
      601 tcacggctga aatgctcggc agcgccgtcc agaccgtgta tgacgtcggc gttgcgggca
      661 tccaccgcgt cctgagcaaa cggaaaattc tcgacgccgc gcgggtcgtc gttgtcgtcg
      721 cgggcatgga cggcgccctg ccttcggtcg tgggcgggct cgtggacaag cccgtgatcg
      781 ccgttccgac gagcgtgggc tacggcgcgg gcttcgaggg cctggcgccg cttcttacga
      841 tgctcaactc ctgcgcggca ggcatcgccg tcatgaacat cgacaacggc ttcggcgccg
      901 gggtcctggc gcacaggata aacctgctgg gggaaaacct cggtgtatga ccgcgaatgc
      961 acgcaaatga gcgcgaataa aaagtcttaa gaaacgagaa tcgggttcgt actcattcgc
     1021 gtctattcgc gtttattcgc ggttgaaaaa ttatgaccct cgcctacttc gactgctttt
     1081 ccggcatatc cggcgacatg accctgggcg ccctcgtgga cgcaggagtc tccatcgacg
     1141 ttctgcgctc cgaactcgcg aagcttaacc ttccgggtta cgagctcaaa tctgaaaagg
     1201 tcaaacgcgc aggcatcgcc gcgacaaaag tccacgtgat catcgggaag aaaggccggg
     1261 agtcgcgcca tctctcggac attacggcaa tcatcaataa ctcgcctctc gcagccggaa
     1321 taaaggagaa aagcgtcggt atcttcaacc gtctcgcgga ggccgaagcc aaagtccatg
     1381 cgacgactcc ggagaaggtc cacttccacg aagtcggtgc cgtcgacgcc gtcgtggaca
     1441 tcgtgggttc cgtcatcggg ctcgatcttc tgggagtcac gcgaatccgg gcctcggccg
     1501 tcaacgtcgg ctccggcacg gtcgagacct ctcacggcct cctgcccgtt cccgcccctg
     1561 ccgcggccga attgctcaaa ggcatcccct tctatcaatc cgaggccagg ttcgagctcg
     1621 ccacgcccac cggcgccgcg atcataagta cgctaggctc ttccttcgga cccctgccgg
     1681 aaatgaaggt gaaccatatc ggctacgggg cgggagacgg ggattttccg gacaggccga
     1741 acgtgctcag gctcatgctc ggtgagccgg cggtcgcata cgatgaagat tcctccatac
     1801 tcatcgagac gaatatcgat gacatgaacc cgcaggtcta tgagtatatc atcgataagc
     1861 tcatgaagca gggagcaaag gacgcatacc ttaccccaat catcatgaaa aaaggaaggc
     1921 ctgcgatttt gctttctgtc ctgacggata cgccctacgt cgatcgcatc gtcgatgcga
     1981 tcttccggga gactacgagc atcggcgtcc gcatcaatga agtgaaaaga aaaaagctca
     2041 accgcgagat cagagtagtc gaaacctctt atggtaaaat caggatcaag gtcagtaaac
     2101 ggggagacga gatactgacg gccacccc
//
LOCUS       DASSLD010000568         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_793969,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000568 DASSLD010000000
VERSION     DASSLD010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793969"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..975)
                     /locus_tag="VFK23_10250"
     CDS             complement(196..975)
                     /locus_tag="VFK23_10250"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET7319506.1"
                     /translation="MVELEHIHSCPNCGGPIEENRYVRRPDGTYRCEHCYFSDTYLEG
                     IVHHIDTAYLVTLEAFVNAIDAREHEVGNHSLRVTEYSLIIANACDVRGRDLVDLYCG
                     ALLHDIGKIGVPDAVLHKPGPLTAEEQIIMRRHPEIGFRIISHIGYFARASEIIRTHH
                     EHFDGSGYPRGLKGDAIPHGARVFAVADALDALTVKRSYHEAVSFEEALEKIIKASGK
                     IYDPAVIQAALRSSVELKEYIGKIVFDGMDLEGGGNARPDL"
     gene            1102..1353
                     /locus_tag="VFK23_10255"
     CDS             1102..1353
                     /locus_tag="VFK23_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HET7319507.1"
                     /translation="MDTMARIKRLFIDKIEVREELLKPETTLESLGLDSLDTIEFLFS
                     LEEEFHIKIDDRSKSITTIQDVVNLIDIIAAEQRPAVRA"
     gene            1364..>2501
                     /locus_tag="VFK23_10260"
     CDS             1364..>2501
                     /locus_tag="VFK23_10260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HET7319508.1"
                     /translation="MRRVAVTGVGVISPVGNTAEVFFHNLAAGRSGIRRLSSNIADRL
                     EVKVAAESTFSGEDYYSKKQLGLLDRTSQMALAAASQAWTDSGLELNDVEKQRSGVYL
                     GTGMGGANSLDEMFGKLYRENAGRVSPLFVAKIMSNAPASHISIQYGLSGPCLTFSTA
                     CSSSAVSIGEAFRQIKAGHADIILAGGAESLLAYGSFKCWESLGVLAPEEAANPAASC
                     RPFSMDRAGFVLGEGAAIAVLEDMERARRRGAQIYAEIAGYGSTSDAHHITSPSVDGQ
                     ARAMRLALEEAGSSIDKIDYINAHGTATAKNDIVETRAIKEVFGGRAKTIPASSTKSM
                     HGHLLGAAGAVEFVAAILAMRHGTIPPTAHLRVPDPECDLDYVPN"
BASE COUNT          529 a          725 c          765 g          482 t
ORIGIN      
        1 cgacgttctc gaccgaggag agagcgagag cgtaccgctg ttcgtccagg gtaaaaggaa
       61 ggagcctgtc aggattattc atgtcacaac cccccgaaag accccattat aagcgaaata
      121 aagatatttt acaacgacag gtaactgcgg gtgcgtgaat accgatgctc cgggagtagg
      181 gcccatgaac gggcgttaca gatccggacg tgcattcccg cccccctcga ggtccatgcc
      241 gtcgaacacg atcttgccga tgtattcctt gagttcgact gatgaccgga gcgccgcctg
      301 gatgaccgcg ggatcgtata tcttcccgga cgccttgatg attttttcca aggcctcctc
      361 gaacgacacc gcctcatgat acgaccgttt gaccgtgagc gcatcgaggg cgtcggcgac
      421 ggcgaagacc cttgccccgt gcgggatggc gtcgcccttg agtcccctgg gatagcccga
      481 tccgtcgaaa tgctcatgat gcgtgcgaat gatctcagat gcgcgggcaa agtagccgat
      541 gtggcttatg atccggaagc cgatttcggg gtgtcttcgc atgatgatct gttcctcggc
      601 ggtcagaggc cccggtttat ggagcacggc gtcaggcacg ccgatctttc ctatgtcgtg
      661 gagaagcgcc ccgcagtaaa ggtccacgag gtccctcccg cggacatcgc aggcgttcgc
      721 aatgatgagg gaatactcgg tcacccggag ggaatggttg ccgacctcgt gctcccgcgc
      781 gtcgatggcg ttcacgaagg cctcgagggt caccaggtag gccgtgtcga tatggtggac
      841 aatgccttcg agataggtgt cgctgaaata acagtgctcg cagcggtagg ttccgtcagg
      901 cctcctgacg taccggttct cctcgatcgg ccctccgcag ttggggcagg aatggatatg
      961 ttccaattca accatgtcgt tagcgtatca gcggcggcgg cgtttgtcaa cggggtggac
     1021 caaagtctta cctttatttc cttagttgga ttataataag agcatcagtc aaaaaacttc
     1081 cgggagggaa ggaaagggct tatggacacg atggcaagga tcaagagact ttttatcgac
     1141 aagattgaag tcagggaaga actgctcaag cccgagacga cgctggaaag cctgggcctc
     1201 gattcgctgg acacgatcga gttcctgttt tccctcgagg aagaattcca catcaagatt
     1261 gacgaccgtt cgaagtcgat cacgaccatc caggacgtgg tgaacctgat tgatatcatc
     1321 gcagcggagc agcggcccgc agtgcgggcg tgaggagccc aacgtgagac gcgtcgccgt
     1381 aacaggagtg ggggtcatct cccctgtggg gaacaccgca gaggtctttt ttcacaacct
     1441 cgctgcaggc aggtcgggca tccgcaggct atcttcaaac atcgctgacc ggctcgaggt
     1501 caaggttgcg gcggaatcga cgttcagcgg agaagactac tattcgaaaa aacaactcgg
     1561 gctcctggac cgcacgagcc agatggcgct tgccgcagcg tcccaggcat ggaccgattc
     1621 cggcctggaa ctgaacgacg tcgaaaaaca gcgctcgggc gtttacctcg ggacgggaat
     1681 gggcggcgcg aactcgctcg atgagatgtt cggaaaactg tatcgggaaa atgccgggcg
     1741 ggtcagcccg ctctttgtgg cgaaaataat gtcgaacgca cccgcctccc acatctccat
     1801 tcagtatggc ctctcaggcc cctgcctcac cttctccacc gcctgctcct cctccgctgt
     1861 gtccatcggc gaggcctttc gccagatcaa ggccggccac gccgacatca tcctcgccgg
     1921 aggcgccgaa agccttcttg cctacgggtc attcaaatgc tgggaatcgc tgggcgtgct
     1981 cgctccggag gaggcggcaa atccggccgc gtcctgcagg cccttttcga tggatcgtgc
     2041 cggctttgta ctcggggaag gcgctgccat cgcggtgctg gaagatatgg agcgggcgag
     2101 aaggcgcggg gcgcagatct acgccgaaat cgcgggctac ggttcgacgt cggacgcgca
     2161 tcatatcacg agcccctccg tggacggcca ggcgcgggcg atgcgcctgg ccctggaaga
     2221 ggcggggtca tcgatcgaca agatcgatta catcaacgcc catgggaccg ccacggccaa
     2281 gaacgatatc gtggaaaccc gggcgatcaa ggaagtcttc ggaggccggg cgaaaaccat
     2341 cccggcaagt tcgacgaaat ccatgcacgg gcatctgctc ggggccgcag gagccgtgga
     2401 attcgtggcg gccatcctcg ccatgaggca cgggaccatc ccgcccacgg cgcacctccg
     2461 ggtgcctgac cccgagtgcg acctcgacta cgttccgaat g
//
LOCUS       DASSLD010000569         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_435585,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000569 DASSLD010000000
VERSION     DASSLD010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435585"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFK23_10265"
     CDS             complement(<1..543)
                     /locus_tag="VFK23_10265"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721345.1"
                     /note="Catalyzes the reversible oxidation of malate to
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET7319509.1"
                     /translation="MRKKVTVVGAGNVGATLAQRIAEARLADVVLVDVVEGVPQGKGL
                     DLLEAGPVLGYDTAITGSNGYEETAGSDIIVITAGLARKPGMSRDDLLAANGDIVKKV
                     AAETGRRSPKAVMIVVTNPMDVMAYVAMKAAGFPRERVIGMGGVLDSARFRTFIAREL
                     GVSVENTHAFVLGGHGDTMVP"
     gene            complement(644..1924)
                     /locus_tag="VFK23_10270"
     CDS             complement(644..1924)
                     /locus_tag="VFK23_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET7319510.1"
                     /translation="MMDTFIAFISDNWFLVGVAGMSVLSAAFSAAAETALVFSNKAHL
                     GQLAESGNRRAATAIGLAQDRDRLHTVLLLAETFLIVLSAVIGTIVAMRAFTRVSVAA
                     VVAVTAVSAIVVFAAKLVPKGLACRNPDGFALAAAPLLRAMVRLLSPAARLLAVTAGV
                     LAGPGMQGAACTTAVTEEDIKAMINLGEERGSLKEEEKELLHKVFEFGDTLASQAMRP
                     RTEIVSVASNATLQEVFALVSEFGYSRYPVIEDTMDTVIGTLYIKDILVAMAAGEVKR
                     EDSIRPFIRQAYFIPENKRVSELLAEMQRERFQLAIVIDEYGGTAGLVTLEDLIEEIV
                     GSIHDELEAEQKDVQIVDEKNFVVSGQSALDEVNELLGTDLQSTDFNTIGGFVFGLFG
                     RMPKAGEQLKYRNLKLEVLEVEGRKIAQVKITKL"
     gene            complement(2052..>2339)
                     /locus_tag="VFK23_10275"
     CDS             complement(2052..>2339)
                     /locus_tag="VFK23_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HET7319511.1"
                     /translation="AVGLATSRDCRIVVKFGEGCTRCRGTGYLGRTGIFEVLEVNEKI
                     RNIIRDKADAELIRKAAIEDGMVVLREAAVKKMLKGETTFDEVIRVTGEKS"
BASE COUNT          406 a          795 c          677 g          461 t
ORIGIN      
        1 cgggaccatg gtgtcgccgt gcccccccag gacgaaggcg tgggtatttt ccacggatac
       61 gccgagctcc cgggcgatga aagtccggaa ccgtgcagaa tcgaggacgc cgcccatccc
      121 gatcacgcgc tcgcggggaa aacccgcggc cttcatggcc acgtaggcca tcacgtccat
      181 cgggttggta acgacgatca tgacggcctt cggcgagcgc ctgcccgttt cggcggcgac
      241 cttcttcacg atgtccccgt tcgccgccag aaggtcgtcg cggctcatgc ccggcttccg
      301 cgccagaccg gccgtgatca cgataatatc ggaccccgcg gtctcttcat agccgttcga
      361 acccgtgatc gccgtgtcgt accccaggac gggccctgcc tccaaaaggt cgagcccctt
      421 gccctgcggc acgccctcca ccacgtcaac gaggacgacg tccgcgaggc gggcctccgc
      481 gattcgctgc gccagcgtcg cccccacgtt ccccgcgccc actaccgtaa ccttcttccg
      541 caaatcacac ctccaaagaa tggcgtagcg cagagcgcag ggagcatgac gtttaaagct
      601 gtttcgcttt gcgccatgcg ccatacgctt tgcggttttc acattacagc ttcgtgatct
      661 tcacctgggc gatctttctc ccctcgactt ccaggacctc gagcttgagg ttccggtatt
      721 tgagctgctc tcccgccttc ggcatccttc cgaacaggcc gaagacgaag ccgccgatcg
      781 tgttgaaatc cgtagactgg aggtccgtgc cgagcagctc gttcacctcg tcgagcgccg
      841 actggcccga gaccacgaag ttcttctcat cgacgatctg gacgtccttc tgttcggcct
      901 cgagttcgtc gtgaatgctg ccgacgatct cctcgatcag gtcttccaac gtcaccaggc
      961 ctgcggtgcc tccgtattcg tcgatgacga tggcgagctg aaagcgctcg cgctgcatct
     1021 ccgcgaggag ttcgctcacg cgcttgttct cggggatgaa gtatgcctgc ctgatgaagg
     1081 gacggatgct gtcctcccgc ttcacctcgc ccgcggccat ggcgacgaga atgtctttga
     1141 tgtagagggt gccgatgacc gtgtccatcg tgtcctcgat cacggggtag cgggaatacc
     1201 cgaactccga cacgagggcg aacacctcct gaagggtcgc gttcgacgcg acactcacga
     1261 tctcggtccg cggccgcatc gcctgggagg ccagcgtgtc gccgaactcg aagaccttgt
     1321 gcagcagttc cttttcctct tccttgagcg acccccgctc ttcgccgaga ttgatcatgg
     1381 ccttgatgtc ttcttccgtg accgcggtcg tgcaggccgc gccctgcatc cccggcccgg
     1441 caagtacgcc ggccgtgacg gccagcaagc gcgccgcggg agacaaaagc ctgaccatcg
     1501 cccgtaaaag cggcgccgcg gcgagggcaa aaccgtcggg attcctgcag gcgagcccct
     1561 tgggcacgag ctttgcggca aatacgacga tggcgctcac ggcagtcacg gccacgaccg
     1621 ccgctacgga gacgcgggta aaggcgcgca tcgcgacgat ggtgccgatc acggcggaca
     1681 gcacgatgag gaaggtctca gccagcagga gcaccgtatg gagacggtcg cgatcctgcg
     1741 ccagcccgat ggccgttgcc gcccggcggt tcccgctttc agcgagctgc cccagatgcg
     1801 ccttgttgga gaagaccagg gccgtctccg ccgccgcgct gaaggcggcg ctgaggacgc
     1861 tcatccccgc caccccaacc aggaaccagt tgtcggatat gaaggctatg aaggtgtcca
     1921 tcattttgat tgcggaatgt ggatggccga tgaaacgggc cacgctgatg cggaatgcgg
     1981 atttaaaaac cgtaatgacg gcgcagcttt tttgccttta ttccgaattc cgcattcggc
     2041 attcctcatt ttcaactctt ctcccccgtc acccggatga cctcgtcgaa cgtggtctcg
     2101 cctttcagca ttttcttcac cgcggcctcg cgcaggacga ccatgccgtc ttcaatcgcc
     2161 gctttgcgga tgagctccgc gtccgccttg tcccggatga tgttcctgat cttctcgttc
     2221 acttcgagga cctcgaagat gccggtgcgt cccaggtaac ccgtgccccg gcaccgggtg
     2281 cagccctcgc cgaacttgac cacgatcctg cagtccctgc tcgtggcgag ccccacggc
//
LOCUS       DASSLD010000570         2974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1467230, whole genome shotgun sequence.
ACCESSION   DASSLD010000570 DASSLD010000000
VERSION     DASSLD010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467230"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1865
                     /gene="glgP"
                     /locus_tag="VFK23_10280"
     CDS             147..1865
                     /gene="glgP"
                     /locus_tag="VFK23_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HET7319512.1"
                     /translation="MNDLHEFTAEPMIAYFSMEIGVKNDIPTYSGGLGILAGDTIKAA
                     ADLKLPLVAVTLLSRKGYFSQEIDSNGWQKEKAVVWDPARHMTLLPEKGRVQIEGRDV
                     YIQAWLYRVVSPTGGKVPILFLDTDLAENAAEDRSITDQLYGGDNVYRIKQEVVLGMG
                     GVRILQALEFHIKKYHMNEGHASFLALELLLRFKRDIESVWDERSVWDITRVRDLCVF
                     TTHTPVEAGHDKFPYTVVRQVLGEIISFDVLKDLAGKDNLNMTHLALSLSKYVNGVAK
                     KHGEVSQHMFPGYHIHAITNGVHTFTWTCEDMARLFDKYIPGWANEPELFVRSGNIPE
                     LELWEAHREAKRKLLNYVGEASGVIMDPNVLTLGFARRATAYKRPHLIFSDIDRLVRI
                     SEKGRIQIIYAGKAHPHDDDGKRIIQSIYGFRDKLRGKIAVAFLPGYDMELALKLVSG
                     VDVWVNTPLRPLEASGTSGMKAAHNGVVNFSVLDGWWIEGHIEGFTGWSIGSKPVETD
                     RPKQSDADDAEDLYNKLENLIIPKYYDPNKSGWSRMMRNAISKNAYYFNCHRMMRRYV
                     TEAYIR"
     gene            1974..2459
                     /gene="rfaE2"
                     /locus_tag="VFK23_10285"
     CDS             1974..2459
                     /gene="rfaE2"
                     /locus_tag="VFK23_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HET7319513.1"
                     /translation="MRGKIKTAQELLPLLGILRATGKKIVFTNGCFDILHTGHTRYLG
                     MARSFGDVLVVAVNSDLSVRTIKGEKRPVNAEAERMEVLAALKCVDFVTLFSEPDPHR
                     IISELQPDVLVKGGDWPVEKIIGGDVVQARGGTVVNVPYIEGASTTGIIERILKLYSK
                     A"
     gene            complement(2499..2906)
                     /locus_tag="VFK23_10290"
     CDS             complement(2499..2906)
                     /locus_tag="VFK23_10290"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HET7319514.1"
                     /translation="MDRNAFEKLVEKSLERLPKKFKQKLRNIMIEIEDRPHQEVLEDM
                     GIEDGLLFGLYQGVPLTEREWNFGNVLPDRIIIYQEPIEQAASSSEEIEEIVLDTVVH
                     EIGHYFGFGDDELYDIEDEKAKKKEGGKRRGKK"
BASE COUNT          678 a          840 c          827 g          629 t
ORIGIN      
        1 aagccgcccc ttcccgggcg ttcgggcaag ccataacgca acctcgcagc acggagcatg
       61 ctccctcagt ctgttgaaat ggagcggtgt ttctgttatc ttaaatccaa ggaacgcgcg
      121 gggaaccatt tttaaaaagg agaagaatga acgacctgca tgaattcacg gcagagccga
      181 tgatcgctta tttttccatg gagataggcg tgaagaacga catccccacg tacagcggcg
      241 gcctcgggat tctggccggc gacacgatca aggcggcggc cgacctgaag ctgcccctcg
      301 tcgccgtgac cctcctcagc cgcaagggat atttttccca ggagattgac tcgaacggct
      361 ggcaaaagga aaaggccgtg gtctgggatc cggcccggca catgacgctc cttcctgaaa
      421 agggccgggt gcagatcgaa ggccgtgacg tctacatcca ggcatggctc taccgggtag
      481 taagcccgac gggcggcaaa gtgccgatcc tgttccttga cacggacctt gcggagaacg
      541 cggcagagga ccggagcatc acggaccagc tctacggggg ggacaacgtc taccgcatca
      601 agcaggaggt ggtcctcggc atggggggcg tccgcatact ccaggccctc gaattccaca
      661 tcaagaaata tcatatgaac gagggccatg caagcttcct cgccctcgaa ctgctcctcc
      721 gtttcaagcg cgacatcgaa agcgtctggg acgaacgatc ggtgtgggac atcacccgcg
      781 tgcgggacct ctgcgtattc acgacccaca cgcccgtcga ggcgggccat gataaattcc
      841 cctataccgt agtgaggcag gtgctcgggg aaatcatctc cttcgacgtt ctgaaggacc
      901 tcgcaggcaa ggacaacctg aacatgacgc acttggcgct cagcctgagc aagtacgtaa
      961 acggcgtcgc caagaagcac ggcgaagtgt cgcagcacat gttcccgggc taccatatcc
     1021 acgccatcac caacggcgtt cacaccttca cctggacctg cgaggacatg gcgcgactgt
     1081 tcgacaagta tatccccggc tgggcgaacg agcccgaact gttcgtccgc tcgggtaaca
     1141 tccccgagct ggaactctgg gaagcgcacc gggaggcgaa acgaaagctc ctgaattatg
     1201 taggcgaagc ctccggagtg atcatggacc cgaatgtcct gaccttgggc tttgcgcgtc
     1261 gagcgactgc gtacaagcgg ccccacctga tcttttccga catagaccgc ctggtccgga
     1321 tcagcgaaaa aggcaggatc cagatcatct atgcgggcaa ggcgcaccct cacgacgacg
     1381 atgggaagcg gattatccag agcatctatg gattccgtga caaactcaga gggaaaattg
     1441 cggtcgcctt tctgccgggt tatgacatgg aactcgcgct caaactcgta tcaggagtgg
     1501 acgtgtgggt gaacacgccg ctcaggcccc tcgaggcgtc gggcaccagc ggaatgaagg
     1561 cggcgcacaa cggagtggtg aacttcagcg ttctcgatgg ctggtggatc gaagggcata
     1621 tcgaagggtt tacgggctgg tcgatcggct ccaagcccgt cgaaacggac cggcccaagc
     1681 agtccgacgc cgatgacgcc gaagaccttt acaacaaact cgagaacctg atcatcccga
     1741 aatattatga tccgaacaag agcggctgga gccgcatgat gaggaacgcc atcagcaaga
     1801 acgcctacta cttcaactgc caccgcatga tgcggcggta tgtgacggag gcgtacatca
     1861 gataaatgcg gaattaaaga ttgttcagaa tccccttctt tgtcccggca aattccggtt
     1921 gatttccttg ttgcttttct tgctttctct cccgttttgt attatagttt cgcatgcgcg
     1981 gcaagataaa gactgctcag gaactcctcc cccttcttgg catccttcgg gcaaccggca
     2041 aaaagatcgt gttcacgaac ggatgtttcg acatccttca caccggccac acgcgctatc
     2101 tcgggatggc cagatcgttc ggcgacgtgc tcgtcgtcgc tgtcaacagc gatctgtcgg
     2161 tacggacgat caagggggag aaaaggccag tcaacgccga ggcggagcgg atggaggtcc
     2221 tggccgccct caaatgcgtt gatttcgtca cgctcttcag cgaaccggac ccgcaccgga
     2281 tcatatcgga actgcagccg gatgtcctgg tcaaaggggg agactggccc gtggagaaga
     2341 tcatcggcgg ggatgtggtt caagcgcggg gcggaacggt ggtgaacgtg ccgtatatcg
     2401 aaggcgcgtc cacgacgggg atcattgaga ggatattgaa actttattcg aaggcatgat
     2461 gtaaaggggt cactttctta cttcttactt tctaatttct acttcttgcc tcttctcttc
     2521 ccgccttcct tctttttcgc tttctcatcc tcgatgtcat acaactcatc gtcaccaaac
     2581 ccgaaatagt gtccgatctc gtgcacgacc gtatcaagaa cgatttcctc gatctcttcg
     2641 gacgaggagg cagcctgctc gatgggttcc tgatagatga ttatccgatc cggaagaacg
     2701 ttcccgaaat tccattcccg ctcggtaaga ggcacaccct ggtagaggcc gaagagcaac
     2761 ccgtcttcga tccccatgtc ttcaaggacc tcctggtggg gccggtcctc gatctctatc
     2821 atgatgttcc ggagcttttg tttgaatttt ttcggcaggc gctccagcga tttctcgacg
     2881 agcttttcaa aggcgttacg gtccatgggc tgatattctt ccttaaagta ctttacggga
     2941 taacatctcg aatttcgcgt ctatgcgaaa taat
//
LOCUS       DASSLD010000571         2954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_500157,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000571 DASSLD010000000
VERSION     DASSLD010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500157"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..769)
                     /locus_tag="VFK23_10295"
     CDS             complement(56..769)
                     /locus_tag="VFK23_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014258323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET7319515.1"
                     /translation="MKKSTIKKWTILAIIVGAIAAVRVFHLDQYLTLASLKGSLDSIK
                     ALYDANPAAVLGAYFAVYVLTAALSLPGAAPLGMLGGALFGFWVATLVVSFASTIGAT
                     LACFVSRFLLRDWIQDRFGDKIARVNEGIEQEGTFYLFSLRLIPLFPFWMINLVMGIT
                     KMSLLKFYWVSQAGMLPGTMVFVNAGKELAKIDSLKGIFSPGLIISFVLIGILPVAVK
                     KAIEFYRPRAKRAALKRTL"
     gene            complement(773..2005)
                     /locus_tag="VFK23_10300"
     CDS             complement(773..2005)
                     /locus_tag="VFK23_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET7319516.1"
                     /translation="MTDKHDVTPPYGMGLLLTLLGVFIGGIGLNLTPCVYPLIPITAS
                     YFGGRTETGGRQGVLLVHGMLYILGLSVMNSALGVSAAFTGKLMGSMLQHPAVLIFVS
                     AVMFFMALNFFGFWELRLPALLTSTVSKTRTGYAQSLFMGLTLGIVAAPCIGPFVIGL
                     LTMVAQKGSPMFGLLIFFTLSIGLGLPLFVLSLFAGNLNKLPRSGEWMVWIRNLFGWI
                     MLAMGVYFIKPLFLRDNLGTYMLALVLLAGGLHLGFINKSGANVRTFANIKKIFGVIA
                     VASALAMTASAVSRGPGVSWHPYSREFFSSAITSQKPIILDFYADWCTPCRQLSDKTF
                     HHAAVVKESGNFTMIKVDLTRESDPDVMELVRKYEVRGVPTVLFLDSRGNELKALQTV
                     DFVPGDEFLLRMKKARES"
     gene            complement(2147..2503)
                     /locus_tag="VFK23_10305"
     CDS             complement(2147..2503)
                     /locus_tag="VFK23_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7319517.1"
                     /translation="MKRIIHIIPVFFIFILSAVQMGVASVNTGQEQSAIAAEAQKGGY
                     KLISPDEIKTEYLRDPGSLFLVDTRQEWAYLMQHIQGAAYLSVTPTWWYPYYPASRSD
                     MKKVLGLDKDKRVTFY"
     gene            complement(2577..2918)
                     /locus_tag="VFK23_10310"
     CDS             complement(2577..2918)
                     /locus_tag="VFK23_10310"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7319518.1"
                     /translation="MIPVEKRLDLLKVISHPVRIKILEDLTQGVKCVSDFEETLDISQ
                     PNVSQHLKLLRSHHIVDYFIDGRLKCYFLKEPLIPDLLELLKKEYEEDIPAPACCPVT
                     KKGTYPGDRRR"
BASE COUNT          724 a          783 c          810 g          637 t
ORIGIN      
        1 gatcgtcttc ttcatgaaag aactctgcct cggcggaagg acgggatagg ccgatttaga
       61 gagttctctt cagagccgct cgttttgcac gggggcgata aaattctatt gctttcttca
      121 ctgccacggg taggatcccg atcaagacaa acgagatgat caggccgggc gagaatatgc
      181 ctttcaggga atcgatcttc gccagttcct ttccggcgtt gacgaaaacc attgtcccgg
      241 gaagcatccc ggcttgcgat acccagtaaa acttcagcag ggacattttc gtgataccca
      301 tgacgaggtt gatcatccag aaaggaaata gaggaatcag ccggagtgag aataaataaa
      361 aggtcccttc ttgttcgatc ccttcattga ctcgtgcgat cttgtcgccg aatctgtcct
      421 gtatccagtc cctcaagaga aatctcgaaa caaagcaggc gagcgtcgcc ccaatcgtgc
      481 tggcaaagga aacgacgagt gtggccaccc agaaaccaaa cagtgccccg ccgagcattc
      541 ccaggggagc tgccccggga agggacaagg cggcggtcag tacgtaaacg gcaaaataag
      601 cccccagtac cgccgccgga ttggcgtcgt agagcgcttt gatactgtcg agcgaacctt
      661 tgagcgaggc aagggtaaga tactgatcaa ggtgaaaaac tcgaacagcg gcaatggccc
      721 cgacgatgat cgcaaggatc gtccactttt tgatcgtgct ttttttcatg cgtcagcttt
      781 cgcgtgcttt tttcatcctc agcaaaaatt catccccggg aacgaagtcc acggtctgca
      841 gcgctttcag ctcattccct cgggaatcaa gaaagagcac tgtcggcacg cccctgactt
      901 catatttccg gaccagctcc atgacatcgg gatcgctctc tctcgtcagg tcgaccttga
      961 tcatcgtgaa attcccggac tccttgacta ccgccgcgtg gtgaaacgtt ttatcgctca
     1021 gctgccggca gggcgtgcac caatcggcgt aaaagtcgag aatgatcggt ttttgcgagg
     1081 tgatagcgct tgagaagaac tcccgggaat aggggtgcca ggatactccc ggtccccgcg
     1141 agacggccga agccgtcatg gcgagagcgg acgcaaccgc gataacgccg aagattttct
     1201 tgatatttgc gaaggtcctg acgttcgccc ctgatttatt gataaaaccg aggtggaggc
     1261 ctccggccag gagtaccagc gccagcatat acgtccccaa gttgtcccgg aggaagaggg
     1321 gttttataaa atatactccc atcgcaagca tgatccatcc gaaaagattc cggatccaga
     1381 ccatccattc gcccgagcgg ggaagcttgt tcaggtttcc ggcaaacaac gagagaacga
     1441 acagcggcag tccgagcccg atgctcagcg taaaaaatat gagcaggccg aacatcgggc
     1501 tgcccttctg ggcgaccatg gtcaaaagtc cgatcacgaa gggaccgatg cagggagcgg
     1561 cgacgatgcc gagcgtcaag cccatgaaaa gggattgcgc atatcctgta cgggttttcg
     1621 atacggtcga ggtaaggagg gcgggcagac gaagttccca gaacccgaag aaattgaggg
     1681 ccatgaagaa catgacagct gaaacgaaaa tcagcacggc agggtgctga agcatggaac
     1741 ccatgagttt tccggtaaag gcggctgaaa cgccgagggc cgagttcatg acggaaagcc
     1801 cgaggatgta gagcatgccg tgaacgagaa gaacaccctg gcgtccgccg gtttcggttc
     1861 ttccgccgaa gtaggaagcc gttatcggaa taagcgggta tacgcacggg gtaagattga
     1921 gaccgatccc tccgataaaa acgccaagga gcgtgagcag caatcccatc ccgtagggcg
     1981 gcgtaacgtc gtgcttatcc gtcaagggag acgacggccc ctgagcaagc gggagcccca
     2041 tctctttcca ttttggcaga ccttccgcaa aacgatatac attcttataa cccagtttca
     2101 cagccaaact ggctgcggcg tgactgcgga ctcacgtgag cccgtctcag taaaaggtca
     2161 ctcgcttgtc tttgtccaga ccgaggactt ttttcatgtc cgaccgggat gcggggtaat
     2221 acgggtacca ccaggtcgga gtaacggaaa gataggcggc cccttggata tgctgcatca
     2281 gataggccca ctcctggcgt gtgtcgacaa ggaaaagaga gccggggtcc ctgagatact
     2341 cagtctttat ctcatcggga ctgatcagct tataccctcc cttttgagcc tcggctgcga
     2401 tagcgctctg ttcctggccg gtatttacgc tggcgacacc catttgaaca gcagaaagaa
     2461 tgaagatgaa aaataccggt attatgtgaa ttattctttt catcgtcccc caacctcccc
     2521 ttccttcgaa ctcgtgcaat gacgctgttg ctgcagcaga tcctgttctc ttttcctcac
     2581 cgtctcctgt caccgggata agtccctttc ttcgtcacgg gacagcaggc cggcgcaggg
     2641 atgtcttcct cgtactcctt tttcagaagt tcgagcagat cggggatcag gggctccttg
     2701 agaaagtagc atttcaggcg accgtcgatg aagtaatcga cgatatggtg agagcgcaga
     2761 agtttcaagt gctgcgacac gttcggctgg ctgatgtcga gggtctcctc gaagtcgctc
     2821 acgcacttca ctccctgggt gaggtcttcg aggatcttta tccggaccgg gtgggagatc
     2881 accttgagca ggtcaagacg tttttcaacg gggatcatag aatcctcctt tgaagtgaat
     2941 acatgcaaac atta
//
LOCUS       DASSLD010000572         3108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_109838,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000572 DASSLD010000000
VERSION     DASSLD010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109838"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2814
                     /locus_tag="VFK23_10315"
     CDS             <1..2814
                     /locus_tag="VFK23_10315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HET7319519.1"
                     /translation="FYAHQPDLNFDSPHVQRSIMKIVDNWLGTGVDGLRLDAVPYLFE
                     REGTNCENLPETHAFLKSLRKHIDEKFKSRMLLGEANQWPEDAAAYFGAGDECHMAFH
                     FPIMPRLFMALQMEDRFPIMDILDQTPAIAESCQWGMFLRNHDELTLEMVTDEERDYM
                     YRVYARDRRTRINLGIRRRLAPLVGNSRRKIELMNILLFSLPGTPIIYYGDEIGMGDN
                     YYLGDRNGVRTPMQWSSDRNAGFSKTDPQKLYLPVIIDREFHYEALNADNQERNPSSL
                     LWWMKRVIAMRRRFTAFSRGDLTFPPSDNPKVLVFTRCHEGETILVVINLSRFSQVVE
                     IDLSKYAGDVPVEVFSLNRFPAIRKDALYVLTLGFHDYYWFSLEKTAATAAAGAEQAL
                     PELDARKSWMDVFEPRMRPRLEENILPSYLKDPRRSGGKARILQRVRVADVLALDREP
                     DSARLIVLETAYADGPPEEYLLPLAYATGEGAAQLLKEVPKAVLARLRVDGRDGVLHD
                     AVYDEGFRANLLRLIAGKKRVRGSSGELDGQPGRTLHYIMKDTALPLPSQPLRVEQSN
                     SSVQYGAKLHMKLYRRVEEGPNPDVEVSRFLTERAGFAAVPPFAGHIEYRRPGRDVTI
                     VAQVQGYVPNQGDAWTYTIDAVRRFFEHVLPEQGGPRELPQALPPILEISPAGLPALL
                     QELIGGVYLEAATVLGRRTAELHRALADGRGDPAFEPEPFSLLYQRSLYQSMRNLLLR
                     VLPLLQKGLKKFPEGTMSAAARVLEKEKDILALFHRILETKITATTIRLHGDFHLSDV
                     LFTGKDFVIIDFEGEPGRALSEKRLKRSPLRDVAGIIRSFHYAASSALLFSGAYRPED
                     VERLEPWADIWSRAVSGVYLNAYLFEVEEAPFVPSDRKEVETLLRCFLMERAVHDLGR
                     ELDNRPDWIVIPIRSIERLLKRA"
BASE COUNT          610 a          969 c          970 g          559 t
ORIGIN      
        1 ttctacgcgc accagccgga cctgaacttc gacagtcccc acgtgcagcg ctcgatcatg
       61 aagatcgtgg acaactggct cggcacgggc gttgacggtc tcagactcga cgccgtgccc
      121 tacctcttcg agcgcgaggg cacgaactgc gagaacctgc ccgagacgca cgcctttctc
      181 aagagcctca gaaagcacat cgacgaaaaa ttcaagagcc gcatgctcct gggcgaggcg
      241 aaccagtggc ccgaggacgc cgcggcgtac ttcggtgctg gagacgaatg ccacatggca
      301 ttccactttc cgatcatgcc gcggctgttc atggcgctcc agatggagga ccgctttccc
      361 atcatggaca tccttgacca gacccctgcc atcgccgaat cctgccagtg gggcatgttc
      421 ctcagaaatc atgacgagtt gacgctcgag atggtcaccg acgaggagcg ggactacatg
      481 taccgcgtct acgcgcggga ccggcggacg cgcatcaacc tcggcatcag aaggcggctc
      541 gcgccgctcg tgggaaacag ccgccgcaag atcgagctca tgaacatcct gctcttctcg
      601 ctgccgggca cgccgatcat ctattacggc gacgagatcg gcatggggga caactattac
      661 ctgggagacc gcaacggcgt ccggactccc atgcagtgga gttcggaccg caacgccggg
      721 ttctcgaaga cggaccccca gaaactctac ctgccggtca tcatcgaccg ggaattccac
      781 tatgaagcat tgaacgcgga caaccaggag cgcaacccct cgtcgctttt gtggtggatg
      841 aagcgcgtga tcgccatgcg caggcgtttc acggccttca gccgcggcga cctcacgttt
      901 cccccttcgg acaatcccaa ggtcctcgtg ttcacgcgct gccatgaggg cgagaccatt
      961 ctggtcgtga tcaacctgtc gcgcttttcc caggttgtgg agatagacct gtcgaaatac
     1021 gcgggagacg ttccggtcga ggttttcagc ctgaaccgct ttcccgcgat caggaaggat
     1081 gcgttgtacg tactgaccct gggcttccat gattactact ggttctccct ggaaaaaaca
     1141 gccgcaaccg ctgccgcagg agcagaacag gcgctcccgg agctcgatgc acggaaaagc
     1201 tggatggacg tgttcgagcc gcgcatgcgg ccgaggctgg aggagaacat cctcccgtcc
     1261 tacctgaaag acccgcgccg gtccggcgga aaagcgagaa tcctgcagcg ggtccgggtc
     1321 gcagatgtgc tcgcgttgga ccgggaaccg gattccgccc ggcttatcgt tctggaaacg
     1381 gcctatgcgg acggtccgcc ggaggaatac ctgttgccgc tcgcctatgc gacgggggaa
     1441 ggggccgccc agctgctcaa ggaggtgccg aaggccgtcc tcgcccggct ccgggtggac
     1501 ggaagggacg gcgtgctcca cgacgccgtc tacgacgaag gtttccgtgc gaatcttctc
     1561 cggctgatcg cgggaaaaaa acgggtccgc ggaagctccg gagaacttga cggccagccg
     1621 ggaagaacgc tccactatat tatgaaagat acggcgctcc ccctgccgtc gcagccgctc
     1681 cgggtcgagc aaagcaactc atcggtccag tacggggcaa agctgcacat gaagctctac
     1741 cgcagggtcg aggaggggcc gaatcccgac gtcgaggtgt cccggttcct gacggagcgc
     1801 gcaggatttg cggcggttcc gccctttgcg gggcacatcg aataccgccg gccaggccgg
     1861 gatgtcacga tcgtcgcgca ggtccagggc tatgtgccga accagggcga cgcctggacc
     1921 tacacgatcg acgcggtccg ccggttcttc gagcatgtgt tgcccgaaca gggaggcccc
     1981 cgcgaactgc cccaggcgct cccgccgatt ctcgagattt ccccggcagg tcttccggcg
     2041 ctcctccagg agttaatcgg cggggtctat ctcgaggcgg ccacggtcct cggcagaagg
     2101 accgccgaac tccatcgggc gctggccgac gggcgcggcg atcccgcgtt cgagccggag
     2161 cccttctccc tgctttacca gcggtcactg taccagtcca tgcggaatct gctgctccgg
     2221 gtcctgccgc ttttgcagaa gggcctgaaa aagttccccg agggtacaat gtccgcggcc
     2281 gcgagggtcc tggagaagga gaaagacatc ctggccctgt tccacaggat cctggagacg
     2341 aagatcacag ccacgacgat ccggcttcac ggcgacttcc acctgagcga cgtcctgttt
     2401 acgggcaagg acttcgtgat catcgatttc gagggggagc cggggcgggc gctgtccgag
     2461 aagcggttga aacgctcgcc gctcagggac gtggccggca tcatccggtc cttccattac
     2521 gctgcctcca gcgcgctcct cttcagcggc gcgtaccggc ccgaggacgt ggagaggctt
     2581 gagccctggg cggacatctg gtcccgcgcc gtgagcggcg tctatctcaa cgcctatctc
     2641 ttcgaggtcg aggaggcccc ctttgttccc agcgaccgga aagaggtcga gacgctgctc
     2701 cggtgtttcc tcatggaaag ggccgtgcac gatctgggcc gtgagctcga caaccggccc
     2761 gactggatcg tgatccccat ccgcagcatc gagcggcttc tgaagagggc gtgagagggg
     2821 ttttttgtca ttctgaacgg agcggagcga agtgaagaat ctggctcagg acaggctccg
     2881 cgaagggtct ggaaatgatt ggaccaaatg tgattcatcg cggaatttac ctgagcggtt
     2941 atatgccaga ttcttcggct ccgcctcaga atgacagcga aaggcctgaa tgacaaactg
     3001 aaccgattcg gcattcgtgc accgggtgct tcaaggagat cgttcatggc ccacccgaaa
     3061 attctggcat tcgtgctggc gggcggcaag ggagaacgcc tcgcgccg
//
LOCUS       DASSLD010000573         2832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1510739, whole genome shotgun sequence.
ACCESSION   DASSLD010000573 DASSLD010000000
VERSION     DASSLD010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510739"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VFK23_10320"
     CDS             <1..894
                     /locus_tag="VFK23_10320"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET7319520.1"
                     /translation="LLLVLEYRGSRVLPIVGWAAALAVIIASGYYLTAYGKEVRVMAR
                     NFYGGLRVTQYNTGTKDEVRTLVHGSVTHGVQYTAPHRRRTPISYYGQGSGVSLAAKY
                     LRRSTLRVGVIGLGAGSLAAYARHGDIFRFYEINPLVEQMARSEFTYLADCPGKVDVV
                     LGDGRLSLEREPDRRYDLLVIDAFSGDAIPVHLLTEQALELYFRRLDPDGILALHITN
                     SHLDIEPVVAKLASALGKQALLVETDGDEERKIYRSQWALLSTRPVNDPEILGKARAL
                     KDRPGLRLWTDDYNNLFQILK"
     gene            complement(917..1714)
                     /gene="ygiD"
                     /locus_tag="VFK23_10325"
     CDS             complement(917..1714)
                     /gene="ygiD"
                     /locus_tag="VFK23_10325"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HET7319521.1"
                     /translation="MASRMPVLFIGHGSPLNIILDNSYTRSLAALGEDLPKPKAILVV
                     SAHWLTKGTFVTCAERPRTIHDFYGFPDELYALRYPSPGAPGEARQVTETVKKVKVSC
                     GNEWGLDHASWAVLIHMYPKADIPVFEMSLSYTFNEWHPKPLQYHYDLAKELSELRRS
                     GVLIIGSGNIVHNLGMLDFENIDAPVFDWAEEFDEKVKAGLESGNHAELIHYRNMGPS
                     SLLAVPTLDHYLPMIYAIALREKDEPLAFVHEGFQYGSISMRCFRIG"
     gene            complement(1738..2199)
                     /locus_tag="VFK23_10330"
     CDS             complement(1738..2199)
                     /locus_tag="VFK23_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319522.1"
                     /translation="MDRERYSRINEEIEGFRKALVRYAQACEWDTFEKKAGILFDYLE
                     SIERAALEKKFFRVFLAILAVVAAAVVSLVQLDGHVFPALLKYRDAIILAAIAACCFE
                     LFFFLNFRTYVKARMSWYRERRERFIRNIRADFAALFSAPGNGQRGLACAA"
     gene            complement(2311..>2832)
                     /locus_tag="VFK23_10335"
     CDS             complement(2311..>2832)
                     /locus_tag="VFK23_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7319523.1"
                     /translation="KTTNRRLVVMGDELQSVNIALAMKQMYTKDITYIPYRLSLPDSA
                     EEVLVEEGINVVSVEPVSIVGGTEIEGIELINGERIACEAIMASFGVRLNDDFLAGLN
                     LKKDAIGFKYIVSGAYESSLKGLYIVGPLNQGQDQVVIAAGEGAVAAIDINKRLLEEK
                     NVGEEANAGVGHA"
BASE COUNT          564 a          864 c          805 g          599 t
ORIGIN      
        1 ctcctgctgg tcctggagta ccgcgggtcg cgggtccttc cgattgtagg atgggcggca
       61 gcgttggcag tcatcatagc gtccggatat tatctgaccg cgtatggaaa agaggtccgg
      121 gtgatggcga gaaacttcta cggcgggctc agggtgaccc aatataacac ggggacgaag
      181 gatgaagttc gcacgttggt ccacggctct gtgacgcatg gagtccagta cacggcgccg
      241 cacaggcgcc gcacgccgat ctcttactac ggtcagggat cgggagtatc tcttgctgcg
      301 aaatacctcc ggcgctcaac gctccgcgtg ggagtcatcg gactcggcgc gggctccctt
      361 gccgcctacg caaggcacgg ggacatcttc cggttctacg agatcaatcc gctcgtggag
      421 cagatggcgc ggagtgaatt cacctatctt gcggactgtc ccggcaaggt cgatgtcgtc
      481 ctgggcgacg gccggctgtc gctcgaacgc gaacccgaca ggcggtacga tctcctggtc
      541 atcgacgcct tctccggcga cgccattccc gtccacctgc tgacggagca ggccctggaa
      601 ctctacttcc gccgcctgga tcccgacggc atcttggcgc tccatatcac gaattcccac
      661 ctggacatag agcccgtcgt cgcaaagctc gcctcagcgc tcggaaagca ggcgcttctc
      721 gttgagacgg acggcgacga ggagcgtaaa atttatcgtt cccaatgggc gctcctgtcg
      781 acccgtcccg tcaatgaccc ggaaatcctg ggaaaagccc gggcccttaa agacagaccg
      841 ggccttcgcc tttggaccga cgactacaac aatctgttcc agatattaaa ataggaggag
      901 ggggggacac cgcaggtcat ccgattcgga aacacctcat cgagatcgat ccgtattgaa
      961 aaccctcatg gacgaacgcg aggggttcgt ccttctccct gagcgcgatg gcataaatca
     1021 tgggaagata atggtcgagg gtcggcactg ccaggagcga ggaggggccc atgttccggt
     1081 agtggatcag ttccgcatgg tttccgcttt ccaggccggc cttcaccttt tcgtcgaact
     1141 cctccgccca gtcaaagacc ggagcatcga tgttttcaaa atccagcatg cccagattgt
     1201 ggacgatgtt accgctaccg atgatcaaga cgccgctccg gcgcagttcg gaaagttcct
     1261 tcgccagatc gtaatggtac tggagcggtt tggggtgcca ttcgttgaac gtataggaaa
     1321 ggctcatctc gaagacgggg atgtccgcct tgggatacat gtgtatcagc accgcccagg
     1381 acgcgtgatc gagcccccat tcgttgccgc aggagacctt cacttttttc accgtctcgg
     1441 tgacctgccg cgcctcgccg ggggcgcccg ggctggggta tcgcagggcg tacaactcgt
     1501 cgggaaagcc gtagaagtcg tggatggtcc ggggccgctc cgcgcacgtg acgaaggtgc
     1561 ccttggtaag ccagtgcgcc gatacgacca ggatggcctt cggtttcgga aggtcctcac
     1621 ccaacgcggc gaggctcctc gtgtaactgt tgtccaggat gatgttcaac ggagagccgt
     1681 gcccgatgaa gagcaccggc attctggaag ccatggatgc gtcccctttc cccgcgctca
     1741 ggcggcgcag gccaggccac gctgcccgtt ccccggggcg ctgaaaagcg ccgcaaaatc
     1801 ggctctgatg tttctgatga aacgttccct tctctcccgg taccaggaca tcctggcctt
     1861 cacatacgtc cggaagttca agaagaagaa cagctcaaaa cagcaggcgg ctattgcggc
     1921 caggatgatg gcatccctgt atttgaggag cgccggaaag acatggccgt ccagttgcac
     1981 gagggagacc acggcggcgg caacaacggc gaggatagcc aggaataccc tgaagaattt
     2041 tttctccagc gccgcccgtt cgatcgactc gagatagtcg aacagaattc ccgccttttt
     2101 ttcgaaggtg tcccactcac acgcctgggc gtaacggacg agggcctttc tgaatccttc
     2161 tatttcttca ttgattctgc tgtaccgttc gcggtccatg gtttttctcc cgcccttcgg
     2221 acctgataaa attatatcac tgcaaaaaaa gcggctcaac cccgcgattg aaccgcctgc
     2281 ccgcagcgac tgccgcatca cgcgtacttc ctacgcatgc cctactcccg cgttcgcctc
     2341 ttcccctacg ttcttttcct ccaggagccg cttgttgatg tcgattgccg ccaccgcgcc
     2401 ctcccctgcc gcgatgacga cctgatcctg cccctgattg agcgggccga cgatatagag
     2461 gcccttgaga gaagactcat acgcgccgct cacgatgtac ttgaacccga tggcatcctt
     2521 tttcaggttg agtccggcga gaaagtcgtc gttcagtcta acgccgaacg aggccatgat
     2581 cgcctcgcag gcgatccgct cgccgttgat caattctatg ccttcaatct ctgtcccgcc
     2641 gacgatgctc acgggctcga ccgagaccac gttgatgcct tcctctacca agacttcctc
     2701 ggcggagtcc ggcaggctga ggcggtacgg aatgtacgta atgtccttcg tgtacatctg
     2761 cttcatggca agcgcgatat tgacggattg aagttcgtcc cccatgacca caagcctccg
     2821 gttcgttgtt tt
//
LOCUS       DASSLD010000574         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1284107, whole genome shotgun sequence.
ACCESSION   DASSLD010000574 DASSLD010000000
VERSION     DASSLD010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284107"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1030)
                     /locus_tag="VFK23_10340"
     CDS             complement(149..1030)
                     /locus_tag="VFK23_10340"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HET7319524.1"
                     /translation="MKILVIGGTGMVGSLVVSGLLKQGVLVRVMSHSPDKLKKLPAGV
                     EGVRADLDDPDTLPAAFDKIDSVFLLTAVGPNETDEGLSAVSAAKAAGIRKIVYLSVA
                     MPEGSERIPHFRSKLPVEKAVRESGIAYTILRPNNFFQNDVWLKDAIMQYSIYPQPIG
                     EKGVSRVDVRDIADCAVNALMKSGHEGRTYEVHGPDILTGEGIGGIYTKYLGREVRYS
                     GNDLDVWGERVRNLMPEFMVPEMRIMFEYFQEKGMVAARDELEKTEKLLGHKPRTFDD
                     FVKEITAKWRSMAKTAA"
     gene            1351..2160
                     /locus_tag="VFK23_10345"
     CDS             1351..2160
                     /locus_tag="VFK23_10345"
                     /inference="COORDINATES: protein motif:HMM:NF026048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HET7319525.1"
                     /translation="MCEFCTKHGEGKIWYKNAANYGQDLLSDLGRRRYIEGFLTSAFS
                     EGFETLGRLETIFRKKGKLPPAVKAAMINKAKTEHFGQVLPIEEIGCVVGKARTVVRM
                     PCACRWNIGKKEERCCYAVSYGPEAWYDSIDMGYFGKASGEGLEELSPDTALAQMKAM
                     EVEGRVHTIWTMMTPFIGAICNCSSEDCLAMLTLSRTSTEIMERADHVARVDETLCTG
                     CGECDAACRFKAVDSHRHGGQSHAHIDPRRCFGCGLCRNVCASGAITLVPR"
BASE COUNT          459 a          679 c          679 g          500 t
ORIGIN      
        1 agttgggggg gatcgggcag agagaggtga ttgtttgcct gtcttccaac ctcttccccg
       61 ctgctgaaat gataacggtc tgataaggca gcgaacatca aaagggaccg cttctttgac
      121 ctggagaagc gaccccggtg ctgccatttt acgcggcagt tttcgccatg ctcctccact
      181 tcgcggtgat ctccttgacg aaatcgtcaa aggtccgggg cttgtggccg aggagcttct
      241 ccgttttttc gagttcgtcc cttgcagcga ccatgccttt ctcctggaag tactcgaaca
      301 tgatgcgcat ttcagggacc atgaactccg gcatcaggtt cctgaccctt tccccccaga
      361 catcgaggtc gttgccgctg tagcggacct cccgtcccag gtactttgtg tagatcccgc
      421 cgatcccttc gccggtcagg atatcgggtc cgtggacctc atacgttcgg ccttcgtggc
      481 cggatttcat gagcgcgtta accgcacagt cggcgatgtc gcgcacgtcg acccggctca
      541 cccccttttc gccgatgggc tgcgggtaga tgctgtactg catgatcgcg tcctttaacc
      601 atacgtcatt ctggaaaaag ttgttcggcc gaaggatggt gtaggcgatt cccgattccc
      661 tgacggcctt ctccaccggc agtttgctgc ggaagtgcgg tattctttcc gatccttcgg
      721 gcatggcgac cgacagatag acgatcttcc tgatcccggc cgcctttgcc gccgacaccg
      781 cagaaagccc ttcatcggtc tcgttcggcc ccactgcagt cagcaggaag acgctgtcga
      841 tcttgtcgaa tgccgcaggg agcgtatcag gatcatcaag gtccgccctc accccttcca
      901 cccctgcagg gagcttcttc agtttatccg gggaatgact catgacccgt acgaggaccc
      961 cctgcttcag caggcccgac accaccaggc ttcctaccat accggttccg ccaatgacca
     1021 gtatcttcat tttacgcctc ctatcactgc tcacaggaag tcttcgtaat ttttttggag
     1081 aagttgcatc gaacgggttg tgaagatgct attgtgagag gatggttata gtgtgacacc
     1141 gctggagtgg caagtcaagg aaaccgagga atatagtgtc cattaggagc acacttcaag
     1201 tccttggcct tccccttctc gaacaccagc tttcggacgc gcagcatgga caggtcccgt
     1261 acaaccggat cgattggtcg gagactttcc cttgttcata cccttgttca tacggcttga
     1321 catcgtccct cctttcgatt atgttgcctc atgtgtgaat tctgtacgaa acacggcgag
     1381 ggaaagatct ggtacaagaa cgcggccaac tacggccagg acctcctgtc cgatctcggc
     1441 cgccggcgct atatcgaagg cttcctcacc tccgctttca gtgaggggtt cgaaacgctc
     1501 ggacggctcg aaacgatctt ccggaaaaaa ggcaagctcc cgccggcggt caaggccgcc
     1561 atgatcaata aggcaaagac ggagcatttc ggccaggtgc tgcccatcga ggagatcggc
     1621 tgcgtggttg gcaaggccag gaccgtcgtg cgcatgccct gcgcgtgcag gtggaacatc
     1681 gggaagaagg aagagcgctg ctgttacgcc gtgagctacg ggcccgaggc gtggtacgat
     1741 tctatcgaca tggggtattt cggcaaggcg tcaggcgaag gactggaaga gctcagcccg
     1801 gacaccgccc tggcgcagat gaaggcgatg gaggtggagg gacgggtcca caccatatgg
     1861 accatgatga cgccgttcat cggcgcgatc tgcaactgct cttccgagga ctgccttgca
     1921 atgctgacct tgtcccggac ctcgacggag atcatggagc gcgccgatca cgtggcccgt
     1981 gtggatgaga cgctctgcac cggatgcggg gagtgcgacg cagcgtgccg tttcaaggcc
     2041 gtggacagcc accggcacgg cggccagtcc catgcccaca tcgatccccg ccgctgcttc
     2101 ggctgcggct tgtgccggaa cgtgtgcgcg agcggcgcga tcacgctggt gccgcggtaa
     2161 gcggcaggag aaaaccatga aggtgataga gcaggaaggg ttgaaaataa ccgttgacga
     2221 cgacgggttc ctcgcgaata tcgtcgactg gacggaacag gttgcgcgcg ttctggccgc
     2281 gctggaaggc atcccggatc tgccggagca caggctc
//
LOCUS       DASSLD010000575         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_762520,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000575 DASSLD010000000
VERSION     DASSLD010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762520"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="VFK23_10350"
     CDS             <1..1249
                     /locus_tag="VFK23_10350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7319526.1"
                     /translation="VLPPKAIRDSQSEIADLRNRLREAEETLEAIRSGDVDALVVSGP
                     HGESVYTLKSAESPYRVLIESMNEGAANVSSEGLVLYCNGRIAHLLGPSFQSIIGRYL
                     RHFVAPADRSVCDALVKKGLRRSVSAELTLMTDHGPLLVNCSCRPLREDNVISVAVVI
                     TDLSGRIKAEQALRKSNEALDQRVRERTAELEQANTRLRGFARQLIEREEKIRKQLSA
                     DLHDALGQNLTFLSINFAMLKDGLPPDVQDRHRARFDEVNDVIEDTGRAVRRIMSDIR
                     PPVLDDYGLPAALRWQCSLFSKRTGIKVIMRINPSLRVPPEIQPPLYRIAQEALTNIT
                     KHARASRATVGLGAAKGRIRLTISDNGRGFKTPRGQIGKAESGWGLTIMRERAEAIGG
                     SFDLDSAPGKGTRIIVEIPRGRG"
     gene            1246..1893
                     /locus_tag="VFK23_10355"
     CDS             1246..1893
                     /locus_tag="VFK23_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7319527.1"
                     /translation="MKTRILIADDHAVVRIGLRALLEAQNDMSVVGTANNGREAVEKT
                     GLLRPDVVLMDISMPDLNGIEATRLICSQHPSVRVVILSMHYTNEFVFRAMRAGARGY
                     LLKESAGGEVVKAVRTVMRGSRYLGKGVELGRDEDAAEKAGRLKSPLDSLSERERETL
                     QLVAEGKTSAEIALALGLSPKSVETYRSRLMKKLGVKTIPALVKFAVQHGVTSLD"
BASE COUNT          481 a          611 c          650 g          398 t
ORIGIN      
        1 ggttctcccc ccgaaagcga tccgcgactc gcagtcggaa atcgcagacc ttcgaaaccg
       61 cctcagggag gccgaggaaa cgctggaggc catcaggagc ggcgacgtcg atgcgcttgt
      121 cgtttccgga cctcacggcg aaagcgtata taccctgaaa agcgcggaat ctccctatcg
      181 cgtcctcatc gaatccatga acgagggtgc cgcgaacgtg tcctcggagg gcctcgtcct
      241 ttactgcaac ggcaggatcg cccacctgct cggcccctca ttccagagta tcattggaag
      301 gtatctgcgc cactttgtcg ccccggcaga ccggagcgtc tgcgatgccc tggtgaagaa
      361 aggtctgcga agaagcgttt ccgcggagct tacgttgatg acggaccacg ggcccctgct
      421 ggtgaattgc tcctgccgcc ctctgaggga ggacaatgtc atatccgtcg cggtcgtcat
      481 cacggacctt tcgggacgca taaaggccga acaggccctg cggaagtcga acgaggcatt
      541 ggaccaacgg gtcagggagc ggaccgcgga gctcgaacag gctaacacaa ggcttcgcgg
      601 ctttgcccgg cagctcatcg agagggagga aaaaatcagg aagcagctct ccgcggacct
      661 gcatgacgcg ctgggacaga accttacctt tctcagcatc aatttcgcaa tgctcaagga
      721 tggtctgccc cccgatgtgc aagacagaca ccgggcaaga ttcgatgaag tgaacgacgt
      781 gatcgaagac accggccgcg cggtcagacg gattatgtcg gacatccggc cgccggtgct
      841 ggacgactac ggactgcccg ccgccctgcg ctggcaatgc agccttttct cgaagcgtac
      901 cgggatcaag gtgatcatga ggatcaatcc gtcgctcaga gtgccgccgg aaatccagcc
      961 gcccctctac cgcatagccc aggaggcgct caccaacatc acgaagcacg cgcgggcgtc
     1021 gcgcgcgacc gtcgggctcg gggccgcaaa ggggagaatc agacttacga tctctgacaa
     1081 cggcaggggg ttcaagaccc cgcgcggtca gatcggcaaa gcggaatccg gctggggact
     1141 gacgatcatg cgtgaaaggg ctgaggcgat cggcggcagc ttcgatctgg attctgcgcc
     1201 gggcaagggg acgaggatta tcgtggaaat accgaggggg cggggatgaa aacgaggata
     1261 ttgatcgccg acgatcacgc ggtggtacgg atcggtctca gggcattact ggaagcgcaa
     1321 aacgacatgt cggtcgtggg aacggccaat aacggccgcg aggccgttga aaaaaccggc
     1381 ctcctccggc ccgacgtggt cctcatggac atctccatgc cggacctgaa cggcatcgag
     1441 gcgacgcggc tcatctgctc gcagcatccg tcggtccgcg ttgtcattct ctcgatgcac
     1501 tacacgaatg agtttgtttt ccgtgcgatg cgtgcggggg cgcgcggtta cctgctcaag
     1561 gaatcggcgg gcggagaggt cgtcaaggcg gtccgcacgg tcatgcgcgg cagccgctac
     1621 ttggggaaag gggtagaact cggccgggat gaagacgccg ccgaaaaggc gggtcgtctg
     1681 aaaagccccc tggacagcct gagcgagaga gaacgggaaa ccctgcagct cgtggcggaa
     1741 gggaaaacga gcgccgagat cgcgctcgcc ctcggcctgt cgcccaagag cgtagaaacc
     1801 taccgcagca gacttatgaa aaaattaggc gttaaaacaa ttccggctct cgtcaaattt
     1861 gcggtccaac acggcgtcac ctcgcttgac tgaggctttc ctgcataaac ccgggcattc
     1921 taataactat atttttacag gtaacaatct aaattattat taatgtgcta ttttagcatc
     1981 aatttattgc gaaataaaaa atcttggccc gtcggcaatc atcatgaaag gcgacgttct
     2041 cctgtctttt atctctgcgg gtaaggcggg gcaatctcga tgttcctgat caaccgggaa
     2101 ccgagagtgc ttcgcctcat ttttttcgcg atgtcagcct
//
LOCUS       DASSLD010000576         4936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_757720,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000576 DASSLD010000000
VERSION     DASSLD010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757720"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFK23_10360"
     CDS             <1..258
                     /locus_tag="VFK23_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010947125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7319528.1"
                     /translation="IGLTKTIAREYANRGITANAVAPGFIDTAMTQALPENVRQELAK
                     QIPMGKLGTPEDVANAVRFLASPWASYITGQVIHVNGGMYM"
     gene            325..561
                     /gene="acpP"
                     /locus_tag="VFK23_10365"
     CDS             325..561
                     /gene="acpP"
                     /locus_tag="VFK23_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7319529.1"
                     /translation="MADVDAKVKKIIAEQLGVPEGDVKPEASFVNDLGADSLDTVELV
                     MALEEEFGVEIPDEDAEKIATVGNAIEYIKAKAK"
     gene            681..1922
                     /gene="fabF"
                     /locus_tag="VFK23_10370"
     CDS             681..1922
                     /gene="fabF"
                     /locus_tag="VFK23_10370"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET7319530.1"
                     /translation="MKKRVVVTGIGMITPVGLDTESSWEGLINGKSGIGPITQFDDKA
                     IPTQIAGEIPGFDPEKYIEPKEIKKMDRFIHLAIAASQMAMDDSGLRITPENADRIGV
                     IVSAGMGGLPAIERVHDTYREKGYRRVTPFFIPMTIINEAAGILSIRYGAKGPNLSIV
                     TACATGTHSIGDAFNWIQRGEADAMIAGGTESCICPLGVGGFNAMKALSTRNDEPHRA
                     SRPFDAERDGFVMGEGAGIVVLEEMESAVKRGARIYAEVIGYGASGDAYHITAPAPNG
                     DGAARCMKMAIRDSGVSPADMGYINAHGTSTKFGDELETTAIKTVFGDHAYKLAVSST
                     KSMTGHLLGAAGGVEAVVSVLALDRGILPPTINLENPDPECDLDYVPNEARQKQVDVA
                     MSNSFGFGGTNACLIFKRLKK"
     gene            1924..2652
                     /gene="rnc"
                     /locus_tag="VFK23_10375"
     CDS             1924..2652
                     /gene="rnc"
                     /locus_tag="VFK23_10375"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET7319531.1"
                     /translation="MPVPSDIQELISLRFRDRDLLERALTHKSYANENRMSAHNERLE
                     FLGDAVLNLVISEHLMETCPDSTEGDLSRLRASVVSEPALASIAREIGLGRYILLGRG
                     EEQTGGRDKDSLLANCLEALIASVYLDAGKDIAAKFIIRFFNEAIKKSCTARGALDYK
                     TELQELCQERLRQLPEYRVISETGPDHQKQFTVEILVRGEVCGRGVGRSKKDAEQKAA
                     KEALEKLNRESATVRRGEGEHGRS"
     gene            2710..3789
                     /locus_tag="VFK23_10380"
     CDS             2710..3789
                     /locus_tag="VFK23_10380"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7319532.1"
                     /translation="MIIPFFIPHAGCPHQCVFCNQTNITGQHSPTRAEDIAVTIDRYL
                     TSRGRKKPVKPVSRGGSLATIGDQPSSIDDPDVQVAFYGGSFTALPLIEQEAYLEAAR
                     PFIASGRIRAIRLSTRPDCISSMVLGLLMRYRVTTVELGAQSMDDRVLTLAGRGHSSR
                     DTVRAVSLLREHRFAVGIQLMPGLPGDSADIFRTTVDTVIGLKPDFVRLYPALVIKGT
                     PLERLYRAGKYDPLTLDAAVSICREAYTKFEGAGISVVRMGLQPTEELERPGTILAGP
                     WHPAFRQLVESSIFLDKMRAALSGESGTRRTAVFAVSPRDVSAAIGQRRGNIERLKKE
                     FGLADVRIVEGGHDGGRGKVVLREN"
     gene            complement(3992..4180)
                     /locus_tag="VFK23_10385"
     CDS             complement(3992..4180)
                     /locus_tag="VFK23_10385"
                     /inference="COORDINATES: protein motif:HMM:NF021636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HET7319533.1"
                     /translation="MCDVKVYVARQGKEELLLEAVNHLRVEQGEVTVRNLFGEEKKVR
                     GEVREVSLVKNRVVVDQE"
     gene            complement(4227..4418)
                     /locus_tag="VFK23_10390"
     CDS             complement(4227..4418)
                     /locus_tag="VFK23_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319534.1"
                     /translation="MKTDPVKNKKQRQTAELKTKPACGTLTYLAECRGVPRKTGSGRK
                     QAPSSFVGKIVYKEPPSSS"
     gene            4574..>4936
                     /locus_tag="VFK23_10395"
     CDS             4574..>4936
                     /locus_tag="VFK23_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319535.1"
                     /translation="MKRQCPAFSIRLLTRTWFVIALLLAAVPSGGDAGPVRERIIYTA
                     LRPANTDIYLFDAPGKAPRRLTEHPALDYNPVFSPDGRWVVFCSERKGNPDLYALDLQ
                     NPGQPRLLTDSDALEDAPV"
BASE COUNT         1104 a         1487 c         1421 g          924 t
ORIGIN      
        1 atcggcctca ccaagaccat agcccgggag tacgccaacc gcggcatcac ggcgaacgcc
       61 gtggcgcccg gtttcatcga caccgccatg acgcaggcac tgccggagaa tgtcaggcag
      121 gagctcgcaa agcagatacc catggggaaa ctgggtacgc ccgaggacgt ggcgaacgca
      181 gttcggttcc tcgcctcgcc gtgggcgtcg tacatcacag gccaggtgat ccatgtgaac
      241 ggcggcatgt atatgtaaca gcactgcgaa gtgcagagtt caaagtacaa agtttaaaaa
      301 gaacatttta agaggaggac gacaatggct gatgtggatg caaaggtaaa aaagatcatt
      361 gctgaacagc tcggcgtgcc cgaaggtgat gtaaaacccg aggcctcatt cgtgaacgat
      421 ctcggcgctg attcgctcga tacggtggag ctggtcatgg ccctggagga agagttcggc
      481 gtggagatcc ccgatgagga cgccgagaaa atagcgaccg tgggaaatgc catcgagtat
      541 atcaaggcga aagcgaaata aggattcacc gggccgcacc ggttcatgat tcaggatatg
      601 agaaaagcca gaagacctct cccgcttccg cgagagaggt ttttttggct tttcggacca
      661 cagtgcagga ggaaccattc ttgaaaaaga gagtagtcgt caccggtatc gggatgatca
      721 ccccggtcgg tcttgacacg gaatcaagct gggaaggact tataaacggc aagtccggca
      781 taggccccat cacacagttc gatgacaagg ccattccgac gcagatcgca ggcgagatcc
      841 ccggcttcga cccggagaaa tatatcgagc cgaaagagat caagaagatg gaccggttca
      901 tccatctggc catcgcggca agccagatgg ccatggatga ctcgggtctt cggatcacgc
      961 cggagaacgc tgaccgcatc ggcgtcatcg taagcgcagg catgggcggg ctgcccgcga
     1021 tcgaaagggt tcacgatacc tatcgggaga aagggtatcg ccgggtaacg ccttttttca
     1081 tccccatgac catcattaac gaggcggcgg ggatactctc catccgctac ggcgccaagg
     1141 gccccaacct cagcatcgtg accgcgtgcg caaccgggac ccattccatc ggcgatgcct
     1201 tcaactggat tcagcgcggg gaggccgatg ccatgatcgc gggaggaacg gaatcgtgca
     1261 tctgtccgct cggggtcggc gggttcaacg ccatgaaggc cctttccacg aggaacgacg
     1321 agccgcaccg ggccagccgt ccctttgatg ccgagcgcga cggctttgtc atgggcgagg
     1381 gagcgggcat cgtcgttctc gaggaaatgg aatccgcggt gaagcgcggc gcgcgtatct
     1441 acgccgaggt gatcggctac ggtgcctcgg gagacgcgta tcacataacg gccccggccc
     1501 cgaacggcga tggcgcagcg cgctgcatga agatggcgat ccgggactca ggcgtctccc
     1561 ctgcggacat gggctatatc aacgcccacg gcacgtcgac gaagttcggc gacgaactgg
     1621 agacaaccgc catcaagacc gtgttcggcg accatgcata caagctggcg gtaagctcca
     1681 caaaatcgat gacgggccat ctcctcggcg ccgccggagg cgtcgaggcg gtcgtctccg
     1741 tccttgccct tgaccgggga atacttccgc ccaccatcaa cctggagaac cccgacccgg
     1801 agtgcgatct cgattatgtc ccgaacgagg cgcggcaaaa gcaggtggac gtggccatgt
     1861 ccaactcctt cggcttcggc ggaaccaacg cctgcctcat tttcaagaga ctgaagaaat
     1921 aatatgcccg tcccttcgga catccaggag ctcatatccc tccggttcag ggaccgtgac
     1981 ctgctcgaac gcgcgctcac gcacaagtcc tacgccaacg aaaaccgcat gtccgcgcac
     2041 aacgagcggc tcgaattcct gggcgatgcc gtcctcaatc tcgtcatcag cgaacacctg
     2101 atggaaacat gcccggactc gacggagggt gacctctcga ggctccgggc atcggtagtg
     2161 agcgagccgg ccctcgcatc gatcgcgcgc gaaattggcc tcggcagata catcctgctc
     2221 ggcaggggtg aagagcagac cggcggcagg gacaaggatt ccctgctcgc gaactgtctc
     2281 gaggcgctca tcgcttccgt ttaccttgac gcgggcaagg atatcgccgc gaagttcatc
     2341 atccggtttt tcaacgaggc catcaagaag agctgcaccg cgcgcggggc gctcgactac
     2401 aagaccgagc tccaggaact ctgccaggaa cgtctccgac agctcccgga ataccgtgtt
     2461 atttccgaaa cgggtccgga ccaccagaag cagtttaccg tcgagatact cgtcaggggc
     2521 gaagtctgcg gcaggggcgt cggcaggagc aagaaggacg cggagcaaaa ggctgcgaaa
     2581 gaggcgctgg aaaaactgaa ccgtgaatcg gcgacggtgc gacggggcga gggggagcat
     2641 gggagaagct gaatcaacca cggtgaactc cccccttccc ccttctccgt gtcgcccctt
     2701 cgatgtttta tgatcatccc cttcttcatc ccccatgccg gctgcccgca tcaatgcgtg
     2761 ttctgcaatc aaacgaacat caccggacag cacagcccga ctcgcgcgga agacattgcc
     2821 gtaacgatcg accgttatct tacctcccgc ggccggaaga aacccgtcaa accggtctct
     2881 cgcggcggaa gtctcgccac aatcggcgat caaccgtcat cgatcgacga tcccgatgtg
     2941 caggttgcct tctacggagg aagtttcacc gccctgcccc tcatcgagca ggaagcatac
     3001 ctcgaagcgg ccaggccgtt catcgcgtcc ggacggatca gggccatacg gctttcaacg
     3061 aggcctgact gcataagcag catggtactc ggcctcctca tgaggtatcg cgtcacgacg
     3121 gtggagcttg gcgcacagtc catggacgac cgggtgctca cccttgccgg ccgcgggcat
     3181 tcctcccggg atacggttcg cgccgtgtca cttctgaggg aacatcgctt cgctgtcgga
     3241 atccaattga tgccgggact cccgggagac tccgcagaca ttttccggac aaccgtcgat
     3301 acggtcatcg ggctgaaacc ggattttgtc cgtctctacc ccgcactcgt gatcaaaggc
     3361 acgccgctgg aacggcttta ccgcgcggga aagtacgacc cgctcaccct ggatgcggcc
     3421 gtttcgatat gccgcgaagc atacacgaaa ttcgaggggg ccggaatatc cgttgtccgc
     3481 atgggtctcc agccgacgga ggagcttgaa cggccgggca cgattctcgc cggcccctgg
     3541 catcctgcct tcagacaact cgtggaatct tcgatttttc tggataaaat gcgcgccgcg
     3601 ctctcggggg agagcggaac gagaaggacc gccgtcttcg ccgtaagccc ccgggacgtc
     3661 tcggcggcta tcggtcaacg gcgtggaaat attgaaagac tgaaaaagga attcggactt
     3721 gcggatgtcc gaatcgtgga aggcggccat gacggcggca ggggaaaagt cgtccttcgt
     3781 gagaactaat cattcctgaa agagtattcg gacgcagata ccaacagtag gcgcttctaa
     3841 gcgaaatcgg ataaacgcag attaagacaa gaaacaaatc gttgagtaat ggaaaacacc
     3901 ttcactcaga tggtgattgg attttcaatc gcgctgtttg ctcttatctg caaaaatctg
     3961 cgttcatcag cgtccaaccg ccgtctccga atcattcctg atcgacgacg acccggtttt
     4021 tcacgagtga aacctcccga acttctcccc ggaccttctt ttcctcaccg aacagattac
     4081 ggacggtcac ctcgccctgt tcgacccgga gatggttcac agcctccagc aggagttctt
     4141 ccttcccctg cctggcaaca taaactttta catcgcacac ggtctcactc tccccttccc
     4201 ccgcaaacgc aaataaaaaa cccgcctcag gaggatgagg ggggctcctt gtatacgatc
     4261 ttgccgacga aagacgacgg cgcctgcttt ctgcctgacc ctgttttacg cggtactccc
     4321 ctgcactccg ccaggtaggt cagcgtgccg cacgccggct ttgtctttag ctccgccgtc
     4381 tgccgttgtt tcttgttctt caccggatcc gtcttcatcg gaagacaccg cgaacgattc
     4441 tttctgcgta ctctataaca taagcatacc atgaaatcaa gaaaaatgat ggaacacgat
     4501 gcgcgcggcg ccctcgagag ctatgcttga atcaggagcc ggttgatgga ccggttcgcg
     4561 agaggatcgc aagatgaaac gacaatgccc tgccttcagc attagactcc tgacgcgtac
     4621 gtggttcgtc atcgcgctgc ttctggcagc ggtcccttcc ggcggtgatg cgggcccggt
     4681 ccgtgaacgc atcatctaca ccgcgctgcg gccggcgaac acggacatct acctcttcga
     4741 tgccccgggc aaagcgccgc gccggctgac ggagcatccc gcgctcgatt acaacccggt
     4801 cttttctcct gacggcaggt gggtcgtgtt ctgctcggag agaaagggca atccggacct
     4861 ctacgccctg gacctgcaga acccaggaca gccccgactg cttacggaca gcgacgcctt
     4921 ggaggatgcc ccggtt
//
LOCUS       DASSLD010000577         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_526625,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000577 DASSLD010000000
VERSION     DASSLD010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526625"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="VFK23_10400"
     CDS             <1..1112
                     /locus_tag="VFK23_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HET7319536.1"
                     /translation="SFTDSIKKVPLSMDIVGRIFNGSFEPKDNQPLFIPEKWMPITGA
                     PINPSARARPQDFIETGFSTIDGLNTLVRGQKLPVFSCAGLPSKEVVASVLRNSRLAD
                     PSKEFIVVFAALGLTFHEYSFYLHVLEDMKTGFAAFVNMADEPVMERLLAPRFALTVA
                     EYLAFEKAMDVLVIITDMTNYCDALREISTAREELPGRRGYPGYMYSDLASLYERAGR
                     IYGLEGSVTMLPVVTMPEDDITHPIPDLTGYITEGQIVLSRELHQRGIFPPVDVLPSL
                     SRLMQKGIGEGRTRADHRKVANLLYKYYAKGRDLRRLEAIVGRDGMTRADILMLDFAD
                     TFERDFVGQGMTRRSVGDTLDAGLAIMKKFSLEIT"
     gene            1219..1863
                     /locus_tag="VFK23_10405"
     CDS             1219..1863
                     /locus_tag="VFK23_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HET7319537.1"
                     /translation="MIHPTRTNLLLLKEKSRSVVNSIAILKARKQALIKEFLAATLPF
                     LRSREDIRRSYGRAIRELALSLGHEGKDGIESLVSATEREFGIDVIEKSIWGLRYKDI
                     LFHDLPVREADKRGYDEFGTTPHVDECALLFEKLLESMLHIAQHECKLKRLGDEILRT
                     TRRIKVLEEMVLPGLKHQVKTITQYIGERERESFYRLKRVKNILSEKEARAGTG"
BASE COUNT          439 a          616 c          583 g          395 t
ORIGIN      
        1 tgtcttttac cgattccatc aaaaaggtcc ccctgtcgat ggacatcgtc ggcaggatat
       61 tcaacggatc cttcgagccc aaggacaatc aacccctatt catcccggag aagtggatgc
      121 ccatcaccgg cgctccgatc aacccctcgg cgcgggcccg tccgcaggac ttcatcgaga
      181 cggggttttc gactatcgac ggcctgaaca cgctcgtccg gggacagaag ctgccagtgt
      241 tctcctgtgc gggactgccg tccaaggagg tggtcgcctc cgtcctgcgg aactcccggc
      301 ttgccgaccc ctcgaaggag ttcatcgtgg tcttcgccgc gctcgggctc acgttccatg
      361 agtactcctt ctacctgcac gtcctcgaag acatgaagac cgggttcgcg gccttcgtga
      421 acatggccga cgagcccgtc atggagcggc ttctcgcccc gcgcttcgcg ctgaccgttg
      481 cggaatatct ggccttcgag aaggccatgg atgttcttgt catcatcacc gacatgacga
      541 actactgcga cgccctgcgg gagatatcca cggcccggga agagcttccc ggcagacggg
      601 gctacccggg ctatatgtac tctgaccttg cgtcgctcta cgaaagggcc ggccggatct
      661 acgggctgga gggctcggtc acgatgcttc ccgtcgtcac catgcctgag gacgacatca
      721 cccatcctat tccggacctc acgggataca tcaccgaagg tcagatcgtg ctttcgcgcg
      781 aacttcacca gcggggcatc tttccgcccg tggacgtgct gccgagcctg tcgcggctca
      841 tgcagaaagg catcggggaa ggccggacgc gggccgacca ccggaaggtc gcaaaccttc
      901 tatacaagta ttacgccaag gggagggacc tccgcaggct cgaggccatc gtgggcaggg
      961 acggcatgac cagggcggac atcctcatgc tcgatttcgc cgatacattc gaacgggact
     1021 tcgtcgggca gggaatgacg cggcgctcgg tcggcgatac cctggatgcg gggctcgcga
     1081 tcatgaaaaa attttcgctg gaaataacgt aagaacaagt tcaaagttca aagtggaaac
     1141 actcgaatgt ccttgcatgc ttttcgcttt tactccgaac tctgaactcc gaactctgaa
     1201 ctgaatttcc gaggtcttat gatccatccc acccgcacaa atctcctgct gctcaaggaa
     1261 aagtcccgtt cggtggtgaa cagcatcgcg atcctgaagg cccgcaagca ggcgctgatc
     1321 aaggagtttc tcgccgccac gctgccgttt ctccggtcgc gggaggacat caggaggtcc
     1381 tacggcaggg ccatccggga gcttgccctt tcgctcgggc acgagggaaa ggacggcatc
     1441 gagtccctcg tcagcgccac agagcgtgaa ttcggcatcg acgtgatcga aaagagcata
     1501 tggggcctcc ggtacaagga catcctgttt cacgatctgc cggtcaggga ggcggacaag
     1561 cgcgggtacg acgaattcgg aaccacgccc cacgtcgacg aatgcgcact cctcttcgag
     1621 aagctcctgg aatcgatgct ccatatcgcc cagcatgaat gcaaactcaa gcggctcggc
     1681 gacgaaatac tccgcacgac gagacggatc aaggtgctgg aagaaatggt cctccccggc
     1741 ttgaagcacc aggtcaagac catcacccag tacatcggcg aacgggagcg cgagtcgttt
     1801 taccggctga agcgcgtaaa gaacatcctg tcggaaaaag aggcccgcgc gggaacggga
     1861 taacgtgcgt gtgcgattca gggttttgac agcggctcct ttgaccatta ccgccttccg
     1921 gcaagctctt ccgcacactc ttcaccgtcc tgccgccgga aaaagtgata caattttata
     1981 acggcagcga gatggtcgta tgctttcggt ccacaaggag ggggaaacag tta
//
LOCUS       DASSLD010000578         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1404413, whole genome shotgun sequence.
ACCESSION   DASSLD010000578 DASSLD010000000
VERSION     DASSLD010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404413"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VFK23_10410"
     CDS             complement(<1..515)
                     /locus_tag="VFK23_10410"
                     /inference="COORDINATES: protein motif:HMM:NF015699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HET7319538.1"
                     /translation="MTGYGRKEVNEADVHFVVEIRSLNNRYLDIQLKSPRAIAGMESR
                     VRKSVQERFSRGRFDIFITRNGERDRSGRLVVNEELAGQYIGALRDLKSRFGLGGDVE
                     LSTVAGLQDIITIAEANEDPETLWQVLSRGLTQALGELERMRAEEGAVLAGDILTRLD
                     TIEGLADHIRA"
     gene            complement(594..995)
                     /locus_tag="VFK23_10415"
     CDS             complement(594..995)
                     /locus_tag="VFK23_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319539.1"
                     /translation="MKATILFTEKGIMSLKALFILLLLFIVVHVSIKLAPMYIDAERL
                     KDEMSVKARLAQNLKDEEIMADLAKKAKDLDLPLGPEDFKLQREDENQRMTIRTKWDV
                     TVHFFFDIYPPFTTKSYHFEPVIQENYSRKF"
     gene            complement(1011..1721)
                     /gene="lepB"
                     /locus_tag="VFK23_10420"
     CDS             complement(1011..1721)
                     /gene="lepB"
                     /locus_tag="VFK23_10420"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7319540.1"
                     /translation="MSEIPAEMQKKKSFYKEWVEPFLIAAVVALFIRQFVVEAFKIPS
                     GSMIPTLTIGDHLLVNKFIYGPRIPFTDSRIFTWQEPKRGDIIVFKYPENETKNFIKR
                     IVGLPGDKIEIKNGKLFINDQPVPVTDEGIYEGGSGEGMNSPYYPKPRLLQEQLGAVK
                     HQILYLHDQSGYNFGPVLVPKDSVFVMGDNRDNSQDSRVWGFVRENKILGKALVIYWS
                     WDGDGRWLRWERIGTLIR"
     gene            complement(1829..>2170)
                     /locus_tag="VFK23_10425"
     CDS             complement(1829..>2170)
                     /locus_tag="VFK23_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808772.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET7319541.1"
                     /translation="DMLLNGEPVDALSLITHRNKASIRGRQLAEKLKEVIPRQLYEVV
                     IQAAIGAKIIARETVRALRKDVTAKCYGGDITRKRKLLEKQKEGKKKMKQVGSVELPQ
                     EAFLAVLKVGE"
BASE COUNT          427 a          651 c          532 g          560 t
ORIGIN      
        1 ttggcgcgga tatggtcggc caacccttct atcgtatcga gtcgcgtcag gatgtcaccc
       61 gccaaaaccg cgccttcctc ggcgcgcatc cgctcaagct cgccgagggc ctgtgtaaga
      121 cctcgggaga ggacctgcca caaggtctcc gggtcctcgt ttgcctcggc tatggtgatg
      181 atatcctgaa gaccagccac cgtcgagagt tcaacatcgc ctcccaaccc gaaccgcgac
      241 ttgaggtccc tcagcgctcc gatatactgc ccggccagct cttcatttac gacgagccgg
      301 ccagatctgt cgcgctctcc gtttcgtgtt atgaatatat caaaacgccc ccgggaaaac
      361 cgctcctgca cgctctttcg cacgcgggac tccatccccg cgatcgcccg gggcgacttg
      421 agctgaatgt caaggtagcg gttgttcagt gaacggattt caacgacgaa gtgaacatcc
      481 gcctcgttta cttccttgcg cccgtatcct gtcatgctcc gtatcactgg ttgttcgcct
      541 ttcaccggga agaacgcgct ccttcttccc cccgacgcgg ggcccgcgtc cgtttaaaat
      601 tttcgcgaat aattctcttg gatgaccggc tcgaaatgat aggacttcgt cgtgaacggc
      661 gggtagatgt caaaaaagaa gtgtaccgtc acgtcccatt ttgtgcggat cgtcatccgc
      721 tggttttcat cttcacgctg caatttgaag tcttccggac cgagcggaag gtccagatcc
      781 ttcgcctttt tcgcgaggtc tgccataatt tcctcgtcct tgagattttg ggccagacgg
      841 gccttgaccg acatctcatc cttcaaacgt tccgcatcta tatacatggg cgcaagtttg
      901 atggagacat gcacgacgat aaacaacagc aaaagaataa aaagagcctt taaactcatt
      961 atgccttttt ctgtgaaaag gatcgtcgct ttcatttttc tctttttccc ttaccggatc
     1021 agggtcccga tccgctccca ccgcagccac cggccgtccc catcccagga ccagtaaatg
     1081 acgagggcct tgccgaggat cttgttctcc ctgacgaaac cccacacccg gctgtcctgg
     1141 ctgttgtccc ggttgtctcc catgacaaaa acagaatcct tcggaacgag gaccgggccg
     1201 aaattatagc ctgactggtc gtgaaggtac agtatctgat gcttgacggc acccagctgc
     1261 tcttgcagga gcctcggctt cgggtagtag ggggagttca tcccttcgcc gctcccccct
     1321 tcgtagatcc cctcgtcggt gactggcacg ggctggtcat tgatgaacag cttcccgttc
     1381 ttgatttcta ttttgtcccc cggcagaccg acgatccgtt tgatgaagtt cttcgtttca
     1441 ttctccgggt acttgaaaac tattatatcg ccgcgtttcg gttcctgcca ggtgaagatc
     1501 ctgctgtcgg taaaaggtat ccggggccca tagatgaatt tgttcacgag caggtggtcg
     1561 ccgattgtca gcgtcgggat catagacccc gacgggatct tgaacgcctc aacgacgaac
     1621 tgcctgataa acagggccac cacggcagcg atgaggaatg gctccaccca ttccttgtaa
     1681 aaggattttt tcttctgcat ttccgccggt atttcagaca tcccgcctcc agaccttgcg
     1741 ctccgcgcaa gggattgtag atttgatatt gaaaatcgta aattttaaaa ttgtgcgcct
     1801 ctccatcaat ttccaatcat gctttctctc attcccccac tttcagcacg gcaagaaatg
     1861 cctcctgggg caattccaca ctgccgacct gtttcatctt cttcttgcct tctttctgtt
     1921 tctcgaggag tttgcgtttt ctcgtgatgt ctcctccgta gcacttcgcg gtcacgtcct
     1981 tccggagggc tctgaccgtc tctcgagcga tgatcttggc tccgatcgcc gcctggatca
     2041 ccacctcgta gagctgccgc gggatgacct ctttgagctt ctcggcaagc tgtcttccgc
     2101 ggatcgaggc cttgttccgg tgcgtgatga gtgaaagggc gtcgaccggc tcgccgttca
     2161 aaagcatgtc
//
LOCUS       DASSLD010000579         6679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1545414, whole genome shotgun sequence.
ACCESSION   DASSLD010000579 DASSLD010000000
VERSION     DASSLD010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545414"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFK23_10430"
     CDS             <1..597
                     /locus_tag="VFK23_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319542.1"
                     /translation="DAEPVVSPDGKQIVFGSQREGDFDIYLMNIDGSNVRRLTDRVGY
                     DGGPWFSPDGKKIVWRAWYPETEEEKALWKECMEKDYIVAVPLDIWVMDADGSNKTRL
                     THNGATNWAPSWHPDGKRIIFSSNMDDWRPKIRKFGSNFELYLINIDGTGLERLTFNK
                     VFDSFPMFSPDGKKIVFGSNRSSLNPHETDIFIADWEE"
     gene            792..4130
                     /locus_tag="VFK23_10435"
     CDS             792..4130
                     /locus_tag="VFK23_10435"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7319543.1"
                     /translation="MNSRLFTSLFLAYLFHATPLPAQTAYTHHDLSVTLSPEGHRLSV
                     KDSVTLPADHPRGIQFTLHKGLSPASPTPGVHIEKRGGKEAAVPLESFAVILPPGLRA
                     FVVTYEGSIYHAVEQTGGEQARGFSDTPGTIAPQGVHLSGSSGWYPDFGSPLVTFELD
                     VKLPAGWDAVSQGERTRHDVRSDATSVRWKSPDPQDAIFLIAAPFTEYSKPAGAVTAM
                     AFLRAPDPGLAGKYLDATVKYLDMYSRLIGPYPYKKFALVENFWETGFGMPSFTLLGP
                     TVIRLPFIINTSYPHEILHNWWGNSVYPDYKKGNWSEGLTAYLADHLLREQQGGGAEY
                     RVATLQKYADYVRGGKDFPLTDFRSRHSPSSEAVGYGKAMMFFHMLRLELGDDNFKKG
                     LREFYGKYKFRFASFHDIMTSFEAVSGRDLGREFRQWVERPGAPELVVRNPAVRREKD
                     GFVLTAALEQAQPGEAYALRVPLAVTMEGRERAFQTTVLMTGKKLDLKVPLPAMPVRL
                     DVDPEFDLFRRLSRDEMPPAISQALGAHKMLVILPSAARGDLLRSYREFSGSLTESGP
                     DKVEVTLDSDIKTLPSDHAVTILGWENRFAPEAFEVLAAYDVSMTDRKIGIGRTTLPR
                     DNHSFVFTARSPKNPDMALCLIASDRSEALPGLGRKLPHYHKYSYLAFEGPEPANVAK
                     GRWPVLASPLTAFVPDERGTAKKIEMAGLAPRGPLISPEPPFAKEHMMETIRFLASED
                     LGGRGFGSPGLDKAADYIAKEFKLAGLEPGGDKGSFFQTFEDRGGQQDRISTLKNVIG
                     VIPGTKPEMKGQSVVIGAHYDHLGLGWPETRDKKPGKVHPGADDNASGVAVLIELART
                     FAKGPRPDRAIVFAAFTGEEAGKHGSKYYVAHEKTYPARNSIGMVNLDTVGRLGKNKL
                     LVLGAGSAKEWPHIFQGAGFVTGVDIELVSEDLDSSDQKSFEGAGVPAVQLFSGPNLD
                     YHRSTDTTDKIDADGLVKVASVAREAVEYLSARERPLTGASTSAGTGSSSKGPRKASL
                     GTVPDFAYQGKGLRLSGVVPGSPAEAAGMREGDIIVRVNSAPVAGLRDFSDILKSLSP
                     GDKVSVTFVRDGKESTVQSQVTER"
     gene            4330..6636
                     /locus_tag="VFK23_10440"
     CDS             4330..6636
                     /locus_tag="VFK23_10440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7319544.1"
                     /translation="MQGQNLRKNSGDGSGESPGQHDAAISYRVLFNQSPDGIVIMAPD
                     GTFIDFNEAAHRQLGYPRDEFAKLRLSDIDPFQKPEEILESIKNVLEKGRDEFEVRHR
                     TAAGEIRDVHIITQTMPLSGRTVFHSIWRDVTERKRAEKALWEAQEKYRDLFENAHDA
                     IFIVDADLRYIDVNKRATEISGFSREEFLAMKITDLIPPEQLPRSQVAFEKLRKYRAY
                     EKFVGKMRTKDGRWLDVEINSSAIIRDDKICGSRDIVRDITDRKRAEDALRMSQNYLQ
                     TIIDTEPECVKLVDRDGCLLMMNRAGLAMIEAGTLDEVKGKSVSDLVSPEYREAFRKN
                     VNGVFEGKSGTLEFEIIGLKGRRLRLETHAVPLFGENDRIIALLGITRDITVRKKLEE
                     ELIKAQKLESVGLLAGGIAHDFNNLLTAVLGYINLAKKHIGADDGAREHLVKAENASL
                     RARDLTRQLLTFSRGGSPVRKTSSLRDVIKESAGFALRGSDVTCEFSIPDDLWLVEMD
                     EGQIGQVVQNLIINANHAMPDGGTIRITCRNVGVPDGILPLKKGDYVRVSVEDRGTGI
                     AKEHLPKIFDPYFTTKPKGSGLGLATSYSIIKNHDGYITVESEPGKGTTFHFYLPASD
                     TRGRAATPRVDEQPSAGGGRILVMDDEEIVRDVVADMLKHLGYEVEVARDGVDMLIRY
                     MEARNSGKTFDAVIMDLTVPGGMGGKEAVRKLLEVDPAVKAIVSSGYADNQIMADYKN
                     FGFAGVIVKPFVINHLAATLDSVIKPSA"
BASE COUNT         1493 a         1980 c         1976 g         1230 t
ORIGIN      
        1 gatgccgaac ccgtcgtgtc ccccgacgga aagcagattg tcttcggctc gcagcgggaa
       61 ggcgatttcg acatctacct gatgaatata gacggttcca acgtgcgcag gcttacggac
      121 cgcgtgggct atgacggcgg cccgtggttc tctcccgacg ggaagaaaat cgtctggcgt
      181 gcatggtatc ccgagacgga agaggagaag gcgctctgga aagaatgcat ggagaaggac
      241 tacatcgtcg ccgtgcccct cgatatctgg gtcatggacg ccgacgggtc gaacaagaca
      301 cggctcaccc acaacggcgc cacgaactgg gcgccttcgt ggcatcccga cggaaagcgc
      361 atcatcttct cgagcaacat ggacgactgg cggccgaaga tccggaaatt cggctccaat
      421 ttcgaactct acctcatcaa catcgacggc acgggcctgg agcgcctcac cttcaacaag
      481 gtgttcgaca gcttccccat gttttctccg gacggcaaaa aaatcgtctt cgggtcgaac
      541 cgcagctccc tgaatccgca tgagacggac atcttcatcg ccgattggga ggagtgacgc
      601 cgtagtgcgg ccttcgaccg caattgattt gataaattcg taaaagtcgg atttaaccgc
      661 ggagacgccg aggcgcagag aaaatcctca aattttcaaa ttgaatttct tcgcgtctcc
      721 acacctctgc gatagataat gatttctacg agaacatcaa aatgagaaat tatgggaaga
      781 tatcgcaaat catgaacagc agattattca cttctctatt ccttgcttac ttattccatg
      841 caactcccct ccccgcccaa actgcctaca cccaccacga cctctcggtc accctctccc
      901 ccgaggggca ccgcctctcg gtcaaggact cggtcacgct gccggctgac catccccggg
      961 ggattcaatt caccctccac aaggggcttt cgccggcatc gcctactccc ggcgtacaca
     1021 tagagaagcg gggtgggaaa gaggcggccg tccccctgga gtctttcgcc gtcatcctgc
     1081 ccccgggcct gcgcgccttc gttgttacgt acgagggaag catttaccat gcagtcgagc
     1141 agaccggcgg cgagcaggcg cggggcttca gcgatacgcc gggcaccatc gccccgcagg
     1201 gcgtgcacct ctccggcagc tccggctggt atcctgattt cggctcgccc ctcgtcactt
     1261 tcgaactcga cgtgaagctc cccgcgggat gggacgcggt gagccaggga gagcgcacgc
     1321 gtcacgacgt aagaagcgac gcaacctccg tacgctggaa atctccggat ccccaggacg
     1381 ccatcttcct cattgccgcg ccctttaccg aatactcgaa acccgccgga gcggtgacgg
     1441 ccatggcctt cctgcgcgcg cccgacccgg gcctggccgg caaatatctt gacgctaccg
     1501 tcaagtacct ggacatgtac agcaggctga tcggccctta cccctacaag aaattcgccc
     1561 tggtcgagaa tttctgggag acgggcttcg gcatgccctc cttcacgctc ctgggcccca
     1621 cggtcattcg tcttccgttc atcatcaaca cctcctatcc ccacgaaatc ctgcacaatt
     1681 ggtggggcaa cagcgtgtac ccggattaca aaaaaggcaa ctggtccgaa ggcctcacgg
     1741 cataccttgc cgaccatctt ctccgggagc agcagggagg cggcgccgaa taccgcgtcg
     1801 ccacgctcca gaagtacgcg gattatgttc gcgggggcaa ggactttccg ctcacggact
     1861 tccggtcgcg ccacagcccg tcctccgaag ccgtgggcta cggcaaagcg atgatgttct
     1921 tccatatgct gcggctcgag ctgggagacg ataacttcaa aaaaggactc cgggagttct
     1981 acggaaaata caagttccgc ttcgcgtcct tccacgacat catgacctcc tttgaagccg
     2041 tctccgggag ggacctgggg cgggaattcc gtcaatgggt cgagcgtccc ggtgcgccgg
     2101 agctcgtcgt aagaaatccg gccgtacgac gggagaagga cggctttgtc cttacggccg
     2161 cgctcgagca ggcccagccc ggagaggcat atgctctccg ggtccccctc gccgtgacca
     2221 tggaaggaag ggagcgcgcg ttccagacga ccgtccttat gaccggtaaa aagctcgatc
     2281 ttaaggtccc tctgcccgcg atgccggtga ggctcgacgt cgatcccgaa ttcgacttat
     2341 tccgcaggct gagcagggac gaaatgccgc cagccatctc gcaggccctg ggagcgcata
     2401 agatgctggt cattctccct tcagccgcgc gtggggatct cctgcgctca taccgtgaat
     2461 tttccggttc cctcaccgag tccgggccgg acaaggtcga ggtcacgctc gacagcgata
     2521 tcaagaccct cccgtccgat cacgcagtca ccatacttgg atgggagaac cggtttgcac
     2581 ctgaagcgtt cgaagtcctt gccgcctatg acgtgtcaat gacggaccgg aagatcggca
     2641 tcggtcggac aacgcttccg cgcgacaatc attccttcgt cttcaccgcc cgctccccga
     2701 agaaccccga catggccctg tgcctgatcg catccgaccg ttccgaagcc ctgcccggtc
     2761 tggggcggaa acttccccac taccacaagt acagctacct cgcctttgag ggaccggaac
     2821 cggcgaacgt ggcaaaaggc aggtggccgg ttctcgcttc tccgctcacg gcctttgtcc
     2881 cggatgaacg aggaacggcc aagaaaatcg agatggccgg acttgcccct cgcgggccgc
     2941 tcatcagccc tgaaccgccc ttcgcgaagg aacacatgat ggagacaata cggttcctgg
     3001 ccagcgaaga ccttggcggg cggggattcg gatcgccggg actcgacaag gccgcggact
     3061 atatcgctaa ggaatttaaa ctggccggac tggaaccagg cggcgacaag gggagctttt
     3121 ttcagacctt cgaggatagg ggcggccagc aggaccgcat ctcgacgtta aagaacgtca
     3181 tcggcgtcat tcccggcacg aaaccggaaa tgaaggggca gagcgtggtc atcggcgccc
     3241 attacgacca cctggggctg gggtggccgg aaacgagaga caagaagccc gggaaggtcc
     3301 atcccggcgc cgacgacaac gcgagcggcg tggccgtatt gatcgaactt gcgcgtacgt
     3361 tcgcgaaagg accgcggccc gaccgggcta ttgtgttcgc agccttcacc ggggaagagg
     3421 cgggaaagca cggctcgaaa tattatgtgg cccatgagaa gacttacccc gcccggaact
     3481 cgatcggtat ggtgaacctc gacaccgtgg ggaggctcgg gaaaaacaag ctcctggtgc
     3541 tcggcgcggg gtcagcaaag gaatggccgc acattttcca gggagcgggg ttcgtaacgg
     3601 gtgtggacat tgagcttgtg tccgaagacc tcgactcgag cgaccagaag agcttcgagg
     3661 gggcgggcgt gcctgcggtc cagctcttct ccgggcccaa cctggattat caccgatcta
     3721 ccgatacaac cgataagatc gacgccgacg gcctggtgaa ggttgcgtcc gtggccaggg
     3781 aggccgtaga atatctctcg gctcgcgaga gaccgctgac cggtgcgtca actagtgcag
     3841 gcaccggttc gtcgtcgaag ggtccgcgca aggcaagcct cggcaccgta ccggacttcg
     3901 cctaccaggg aaagggcttg aggctctcgg gtgtcgtacc gggctcgcct gccgaggcag
     3961 cgggaatgag agagggagac atcatcgtgc gggtgaattc ggccccggtc gccggcctca
     4021 gggatttttc cgatatcctg aaatcgctca gtcccgggga caaggtctcg gtcacctttg
     4081 tgcgggatgg gaaggaaagc acagttcaat cccaggtaac agaacgctga agcgactgcc
     4141 gttccacggg gtcattcctc tagggcccgg ccatgcggtc aaaccggaat cgtttgctgc
     4201 gctttttccc tgatggcgat ttgaagcaaa aagtgatata atgagcctta ctgtgcgtcc
     4261 ctgactcttc atcgcttccg aacctcactt ctgacggaac cggtggaatg ccggatgcag
     4321 gagtcgatta tgcagggaca aaacctccga aaaaactcgg gcgacggatc cggagaaagc
     4381 ccgggccaac acgacgccgc aatttcctat cgcgtcttat tcaatcaatc gcctgacggc
     4441 atcgtcatca tggcccccga tggaacgttc atcgatttca atgaagcggc ccaccgccag
     4501 ctcggctacc cacgggacga attcgcgaaa ctccgtctct ccgatatcga cccttttcag
     4561 aaaccggagg aaattctgga gagcatcaaa aacgtcctgg aaaaagggcg tgacgaattc
     4621 gaagtcaggc acaggaccgc cgccggcgaa atcagggacg tccacatcat cacgcaaacg
     4681 atgcccctgt ccggccgcac ggtctttcat tccatatggc gagacgtcac cgagcggaaa
     4741 cgcgcggaga aggccctctg ggaggcgcag gaaaaatacc gcgacctgtt tgaaaacgcg
     4801 catgacgcca tcttcatcgt cgatgcggac ctgcgttaca tcgacgtaaa caagagggcc
     4861 accgagattt ccggcttttc acgggaagag ttcctggcga tgaagattac cgatctcatc
     4921 ccgcccgagc agctgccccg atcgcaggtc gcgttcgaaa aattacggaa ataccgggcg
     4981 tatgaaaagt tcgtcggaaa gatgcgcaca aaggacggac gctggctgga tgtcgaaatc
     5041 aactcgtcgg cgatcatacg ggacgacaag atctgcggct cgcgggacat cgtgcgggac
     5101 atcacggaca gaaaacgggc cgaggacgcg ctccggatga gccagaatta tctgcagacc
     5161 atcatcgata cggagccgga atgcgtcaag cttgtggatc gggacggctg cctgctgatg
     5221 atgaaccgcg cggggctggc catgatcgag gccgggacgc tggacgaggt caaggggaag
     5281 tcggtaagcg atttggtgtc gccggagtat cgcgaggcgt tcaggaagaa tgtgaacggg
     5341 gtcttcgagg gtaaaagcgg aacgctcgaa ttcgaaatta tcggcctcaa gggaaggcgg
     5401 ctccggctgg aaacgcacgc cgtccccctc ttcggcgaga acgacaggat catcgccctg
     5461 ctcggcataa cccgcgacat caccgtgcgc aagaaattgg aggaagagct gatcaaggcc
     5521 cagaagctcg aatcagtcgg cctgcttgcc ggcggcatcg cgcacgattt caacaacctg
     5581 ctgacagcgg ttttaggcta catcaacctc gcaaaaaagc atatcggcgc cgacgatggc
     5641 gcacgtgagc acctggtcaa ggcggaaaac gcttctctcc gggcgaggga tctgacccgg
     5701 cagcttctca ccttttcccg gggagggtcc ccggtgagga aaacgtcttc tctcagggat
     5761 gtcataaagg agtcggccgg ctttgccctg aggggctccg atgtcacgtg tgagttttcc
     5821 attcccgacg acctgtggct ggtggaaatg gacgagggac agatcggcca ggtcgtgcag
     5881 aatctgatca tcaatgccaa ccacgccatg ccggacgggg gaacgatcag gataacatgc
     5941 aggaacgtcg gcgtcccgga cggcattctg ccgctgaaga agggggatta tgtccgggtg
     6001 tccgtcgaag accgggggac gggtatagcg aaagagcacc ttccgaaaat attcgaccct
     6061 tatttcacga caaagccgaa aggcagcggc ctcgggctcg ccaccagcta ttccatcatc
     6121 aagaaccacg acggatacat cacggtggaa tcggaaccgg gaaagggaac gacctttcat
     6181 ttttacctgc ccgcgtcaga cacgcggggc cgcgcggcca cgccccgagt cgacgagcag
     6241 ccttccgccg gaggggggag gatcctcgtg atggacgatg aagagatcgt gagggacgtc
     6301 gtcgccgaca tgctgaaaca tctgggatat gaggttgagg ttgccaggga cggggtagat
     6361 atgctgatcc ggtacatgga ggccaggaat tcgggcaaga cgtttgatgc ggtcatcatg
     6421 gatttaacgg tcccgggcgg tatgggcggc aaagaagccg tcaggaaact cctcgaagtc
     6481 gaccccgcgg tgaaagcgat cgtatcgagc ggttacgcgg acaaccagat catggctgat
     6541 tataaaaact tcggatttgc cggtgtgata gtaaaaccct tcgtgatcaa tcacctggcc
     6601 gcgacactgg attcagtaat aaaaccttcc gcctgaacgc cccttccttg agtccgtgat
     6661 cgtccgcgga aaaccgggc
//
LOCUS       DASSLD010000580         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1251711, whole genome shotgun sequence.
ACCESSION   DASSLD010000580 DASSLD010000000
VERSION     DASSLD010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251711"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFK23_10445"
     CDS             <1..367
                     /locus_tag="VFK23_10445"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET7319545.1"
                     /translation="ADAARILDALGMDTVFLETTGVGQSETAVKRAVHTVVVVVAPGL
                     GDDVQMMKAGIMEIGDIFLVSKGDLKSAAGTMRALEDTIPLLFHGRTPPVVLASAATG
                     TGMDRVLEEVEKHRQSRRN"
     gene            464..1027
                     /locus_tag="VFK23_10450"
     CDS             464..1027
                     /locus_tag="VFK23_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7319546.1"
                     /translation="MDLFEAIRTRRSVRKYSDRPVEDEKLNAVLEAVRMAPSWANYQC
                     WRLVVVKDGPTKEKISSLSYVESYFAPKGYRSNPSMKALAGAPVVIVLCADPERSGAM
                     RGQDYYLVDAGLAAQNLMLAACGQGLGSVFVGVYDEQKLKDLLNIPASIRIVGLFPLG
                     YPAEEKKEGPARMPVSEFCHHGKWGGK"
     gene            complement(1138..2529)
                     /locus_tag="VFK23_10455"
     CDS             complement(1138..2529)
                     /locus_tag="VFK23_10455"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7319547.1"
                     /translation="MSFLKLQLIEPIQKAIKLCGYAAPTPIQEQAIPQVLAGRDLIAT
                     AQTGTGKTAAFVMPALQRLSVPSTSTKRSKGPRVLVLTPTRELANQVTDAVRTYGRFM
                     RVRCGAILGGMPYFEQQRLLSQPVDLVVATPGRLIDHMERGRIDFSRLELLILDEADR
                     MLDMGFSEAVDTIAGATPADRQTLMFTATMDNAMAKLAQRLLSDPVRVEIAGKKTTLE
                     AIEQRLHAADDMQHKNRLLNHLISDSGMTRAIIFSGTKRNADQLARELTAQGHSAAAL
                     HGDMNQNARNRTIMNMRRGKVRLLVATDVAARGLDVTGITHVINYDMPKNAEDYVHRI
                     GRTGRAGASGIAISFASSKEVDFLHQIERYIGQTIPQQVIPGLEPARPLRRSSSGSSQ
                     GPSSGKRWQGATAGRRYGQNTASKNSFGSRSNKKWNGESRFNNDEKKYVKRDGGKFSD
                     FRPARPARQNRWA"
BASE COUNT          487 a          777 c          879 g          568 t
ORIGIN      
        1 agcggacgcc gcccgtatcc tggacgccct cggcatggac acggtctttc tcgagacgac
       61 gggcgtcggc cagagtgaaa ccgcggtgaa gcgggccgtt catacggtgg ttgtggtcgt
      121 cgcgccgggc ctcggcgatg acgtccagat gatgaaggcc gggatcatgg agatcggcga
      181 tatctttctc gtgagcaagg gcgatttgaa aagcgcggcc ggcaccatgc gggccctgga
      241 ggacaccatc ccgctgcttt tccacggacg gacccctccg gtcgtccttg cctccgccgc
      301 cacggggacg ggcatggaca gggtcctcga ggaagtcgaa aaacatcgac aatcccgacg
      361 gaactagcgt actgagtcca gggattttgt tcgtgtcttc gagtcttcgt aatgaatatt
      421 atttttcgtc cccggtttcc ccggggcgag gcagggaggc agtatggacc ttttcgaagc
      481 gatcaggacg cgcaggagcg tccgcaagta ttccgacagg cctgtcgagg acgagaaact
      541 caatgccgtt cttgaggcgg tgcgcatggc gccgtcgtgg gcgaactacc agtgctggcg
      601 tctggtggtg gtgaaggacg gccccacgaa ggaaaagatc agctcgctgt cctatgtgga
      661 atcatacttt gcacccaagg ggtacaggtc gaatccctcg atgaaggccc ttgccggggc
      721 gcctgtagtc attgtcctgt gcgcagaccc tgaacgttcg ggcgccatgc gaggacagga
      781 ctattatctt gtcgacgcgg gacttgcggc gcaaaatctg atgctggccg cctgcggcca
      841 gggcctgggt tcggtgttcg tcggcgttta cgacgagcag aagctgaaag acctgctcaa
      901 tattcccgcg tcgatcagga tcgtgggcct tttccccctg ggatatcccg ctgaagagaa
      961 aaaggaagga ccggcccgga tgccggtctc cgaattctgc caccatggga agtggggagg
     1021 gaagtagccg gcaagccgcg gcctttggtc gtggcaattg aagattgaaa actggaaatg
     1081 cagaaaaaaa aggcgccccg actttcgggg cgcctttttc aatgagatct gatgtattta
     1141 cgcccagcgg ttttggcggg ccggccgggc cgggcggaag tccgaaaact ttcctccgtc
     1201 gcgctttacg tacttttttt cgtcgttatt gaaacgagac tccccattcc actttttgtt
     1261 gctcctggaa ccgaaggaat tcttcgacgc cgtgttctgg ccatagcgcc tgcctgccgt
     1321 agccccctgc cagcgtttgc ctgatgaagg accctgactg ctcccgctgc ttgagcggcg
     1381 cagtggacgc gccggctcca gccccgggat cacctgctgg gggatggtct gcccgatgta
     1441 gcgctcgatc tggtgcaaaa aatcaacttc ctttgacgat gcgaaggaga tggcgatgcc
     1501 cgacgcacct gctcttcccg tacgaccgat gcggtggaca tagtcttccg cgttcttcgg
     1561 catatcgtaa ttgatcacgt gggtgatgcc ggtcacatcg agcccgcgcg cggccacgtc
     1621 cgtggccacg aggaggcgga cttttccccg cctcatgttc atgatcgtcc ggtttcgggc
     1681 gttctggttc atgtcgccgt gcagggccgc ggccgagtgg ccttgggccg tcagctcgcg
     1741 cgcaagctgg tccgcgttcc ttttagtccc ggaaaagatg atggccctgg tcatgccgct
     1801 gtcggagatg aggtggttca ggagcctgtt cttgtgctgc atgtcgtcgg ccgcgtggag
     1861 ccgctgctcg atcgcctcga gcgtggtctt ctttcccgcg atctcgaccc gcacggggtc
     1921 cgagagcagg cgttgtgcaa gcttcgccat ggcgttgtcc atggtcgccg tgaacatgag
     1981 ggtttggcgg tcagctgggg tcgccccggc gatggtgtca acggcctcgc tgaagcccat
     2041 gtcgagcata cggtccgctt cgtcgagtat gagaagctcc aggcgcgaaa agtcgatgcg
     2101 gccccgttcc atgtggtcga tgagtcggcc gggggtcgcc acgacgaggt ccaccggctg
     2161 cgagagcagg cgctgctgct cgaaataggg catgccgccg aggatcgcac cgcacctcac
     2221 gcgcatgaac ctgccgtatg ttctgaccgc gtcggtcacc tggttcgcaa gctcgcgcgt
     2281 aggggtgagc accagcacgc gggggccttt ggaccttttc gtcgaggttg acggcacgct
     2341 caagcgctgg agcgcgggca tcacgaaggc cgcggtcttg ccggtcccgg tctgggccgt
     2401 ggcgatcagg tcacggccgg ccaggacctg gggaatggcc tgctcctgga tgggcgtcgg
     2461 cgcggcgtag ccgcacagtt taattgcctt ctggatcggt tcgatgagct gcagttttaa
     2521 aaaagacatg actctccttt gatctctcat gccggaacaa aggcttccgg aatcggaaat
     2581 ccgttaaagg ttggcatgcc ggccgcaggg acacacgggt atccccgaga gcgaagatga
     2641 cgccggcaca actatgacgg cataactgca tcgttcgcca accggaggtg ctgatgcttt
     2701 ttcagagagg g
//
LOCUS       DASSLD010000581         3964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1466348, whole genome shotgun sequence.
ACCESSION   DASSLD010000581 DASSLD010000000
VERSION     DASSLD010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466348"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..176
                     /locus_tag="VFK23_10460"
     CDS             9..176
                     /locus_tag="VFK23_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET7319548.1"
                     /translation="MDKPWVREGKIVIRKVLPLSLNIDHRVNDGAGAAKFLSRLISYL
                     EDPASLFIESA"
     gene            192..812
                     /locus_tag="VFK23_10465"
     CDS             192..812
                     /locus_tag="VFK23_10465"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HET7319549.1"
                     /translation="MAKAGGKKRTSIVVGIDLAGSPLRNTGICALRGMTVTSIDTLHT
                     DQEIFDYIERIRPGLIAVDAPLSLPPGRKSLEERNAEHFRPSDRELMKRGIRFFPITL
                     GPMRMLTARGIGLKKKLVRRGYAVIEIYPGAAQDIFGIGRKQHGLLKLFRGLKRLGLK
                     GLKREMNGDELDAATGALVGRMYLKEKAEVLGDVKTGAIVVPKQMR"
     gene            complement(865..2034)
                     /locus_tag="VFK23_10470"
     CDS             complement(865..2034)
                     /locus_tag="VFK23_10470"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET7319550.1"
                     /translation="MAEEVVIVSAVRTAVGKFGRSLKDVPAPQLGAVVIAEAVKRAGV
                     RVDQVDEVIMGNVLSAGLGQNPARQAAIHAGIPVETGSVTINKVCGSGLKAVMLAAQA
                     AKAGDADIIVAGGMENMSAAPHLVRNIRWGVKFGDKSLVDSMLYDGLWDVYNNFLMGM
                     TGERVAERFHISREEADRFSVESNHKAATAIREEKFREEIVPVDIKGRAFDTDECVRE
                     DTSLEKLAALSPAFKQNGILTAGNSSKLSDGAAALVVTSAKAAKAAGIKPLARIVDYA
                     TGGTRPEDVMEAPIPTVRKLLAKTGLSMDDIDLVEHNEAYATASIAVSRALGIDSQKL
                     NVHGGAVALGHPIGCSGARVLATLIHALKDRGLKRGLATLCLGGGNAVAMIVERQ"
     gene            2306..2587
                     /locus_tag="VFK23_10475"
     CDS             2306..2587
                     /locus_tag="VFK23_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET7319551.1"
                     /translation="MKRIVVAAVLLAFIVCPAFAFAADTVTLKAKMGDVTFNHKVHGE
                     KTDCKTCHGEGTPGKLNLGGKEPAHKLCMDCHKEKKAGPTKCNDCHKKK"
     gene            complement(2720..2935)
                     /locus_tag="VFK23_10480"
     CDS             complement(2720..2935)
                     /locus_tag="VFK23_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319552.1"
                     /translation="MSTILLRCRDADMMGPVCDYISEEDPFFDKVKDMSAHAQVHNEP
                     EITDDDVAELQELLTGGDLCCEDECCC"
     gene            complement(3130..3693)
                     /gene="frr"
                     /locus_tag="VFK23_10485"
     CDS             complement(3130..3693)
                     /gene="frr"
                     /locus_tag="VFK23_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET7319553.1"
                     /translation="MLSEQKKKAEDKMNKALDALRKEFATLRTGRASLGMLDGIVVDY
                     YDTPTPLNQVANMAVPDPRQITIQPWEPKMLGEIEKAILKSDIGITPTNDGKIIRLNI
                     PSLTEERRQQIVKHAKKLAEDARIAVRNIRRDVNDEIKKKSKDKNAHVSEDETKKLQD
                     DIQKTTDSYIHKIDELLAHKEKEIMTV"
     gene            complement(3686..>3964)
                     /gene="pyrH"
                     /locus_tag="VFK23_10490"
     CDS             complement(3686..>3964)
                     /gene="pyrH"
                     /locus_tag="VFK23_10490"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998099.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET7319554.1"
                     /translation="MEIGAEVIMKATKVDGVYSADPVKDKTATKFDRLTYIDVLQKDL
                     RVMDATAISLCMDNNLPIIVFNLNVPGNIKKIVMGETIGTLVSGRNNA"
BASE COUNT          810 a         1146 c         1069 g          939 t
ORIGIN      
        1 ggaggattat ggacaagccc tgggtcaggg aaggcaagat cgtcatccgg aaggtccttc
       61 ccctgtcgct taacatcgac caccgggtga acgacggggc aggggcggcg aagttcctct
      121 ctcgtctcat cagctatctc gaagacccgg cgtcgctctt catcgaaagc gcatagcgct
      181 ctcccgtgac gatggccaag gccggcggaa agaaacgaac ctctatcgta gtcgggatag
      241 acctggcagg aagccctctt cggaacaccg gtatctgcgc tctccgcggc atgaccgtaa
      301 cgtcgatcga tacacttcat actgatcagg aaatcttcga ctacatcgaa cgcatccggc
      361 ccggtctgat cgccgtcgat gcgcccttga gcctcccgcc cggaaggaaa tctctcgaag
      421 aacggaacgc cgaacacttc cggccgtcgg accgcgaact catgaagcgg ggcatccgct
      481 tttttcccat caccctgggg cccatgcgca tgctcaccgc gcgcggcatc ggactgaaga
      541 agaagctcgt ccggcgcgga tatgccgtca tcgagatcta tcccggcgca gcccaggaca
      601 ttttcggcat tgggcggaag cagcacggac ttttgaaact tttccgggga ctgaaacgac
      661 tagggctcaa gggattgaag agggaaatga acggcgacga actcgatgcc gcaaccggcg
      721 cgctggtcgg aagaatgtac ctcaaggaaa aagcggaagt cttgggggat gtgaaaaccg
      781 gtgcgatcgt ggtgccgaag cagatgagat aacattaaag atgagttgta tttttaacgg
      841 tctgccgaca aagaaaatct tacgctactg tcgttcaacg atcatcgcga ccgcgttgcc
      901 gcctcccagg cacaaggttg cgagaccccg ttttaaaccc ctgtccttca acgcgtggat
      961 cagggtcgcg aggacccggg caccgctgca gccgatcgga tgccccaggg cgacggcgcc
     1021 tccgtggacg ttcagtttct gagaatcaat gcccaaagcc cgcgagaccg caatcgatgc
     1081 ggtggcataa gcttcgttgt gctcgaccag gtcgatatcg tccatgctca agccggtctt
     1141 tgcgagcagt ttcctgaccg tcgggatcgg cgcctccatc acatcttccg gtcttgtccc
     1201 gcccgttgca tagtcgacaa tgcgagccag cggtttgata cccgctgcct tggcggcctt
     1261 ggccgaagtg actaccaggg ctgcagcgcc gtcagagagt ttcgaactgt ttcctgcggt
     1321 aagaattccg ttttgcttaa acgccgggga aagggccgca agcttctcca gagaggtgtc
     1381 ctcccggacg cattcgtcgg tatcaaaggc ccggcctttg atatcgacgg ggactatctc
     1441 ctccctgaac ttctcttcgc gaatggcggt ggcagccttg tggttgcttt ccacgctgaa
     1501 tcggtctgcc tcttcacggg agatatggaa tctttcagcg acccgctctc ccgtcatacc
     1561 catgaggaaa ttattgtaca cgtcccataa gccgtcgtag agcatggagt cgacaaggga
     1621 cttgtcgccg aatttcacgc cccagcggat gtttctgacg aggtgggggg ccgcgctcat
     1681 gttttccatg ccgcctgcca cgatgatgtc cgcgtcgccc gcttttgcgg cttgggccgc
     1741 caacatgaca gccttcagtc ccgacccgca caccttgttg atcgtgacag agccggtttc
     1801 aacagggatc cccgcatgga tagcggcctg acgtgcgggg ttctgtccca gtcccgcgga
     1861 gagcacgttg cccatgatca cctcgtcgac ctgatcaacg cggactcccg cgcgtttcac
     1921 cgcctccgcg atcacgacgg cgccgagttg cggggcaggg acatctttca gggacctgcc
     1981 gaacttgccg acagccgtcc tcaccgcact aacgataaca acctcttcag ccatggctcc
     2041 tccttcagga ctaagtatat aacattccgg gccagctcgt tctccctggg aagagcgccg
     2101 gccgaacgga aaaatatttc ttatctcaat agaatgcaac attgatcaac tattcgttca
     2161 agtgtgtttc ctttctaata ttaaagtcgc ttttcttgcc aattcggcgc gcgtctgcta
     2221 cattcacagg aagcagtcct atttcggatc atgggttggg gacatcgtgc gcgggaacaa
     2281 tatctcacta taaggaggag tatgtatgaa gaggattgta gttgctgcag tgttgttggc
     2341 gttcatcgtc tgcccggctt ttgcctttgc cgcggacacg gtgacgctga aggcgaagat
     2401 gggggatgtg acgttcaatc acaaggtcca cggcgaaaaa acggactgca agacctgcca
     2461 cggcgaaggg actccgggca agctcaacct gggcggcaag gaacccgcgc ataagctctg
     2521 catggattgt cacaaggaaa agaaggcagg accgacaaag tgcaacgatt gccacaaaaa
     2581 gaaataggat gacgaattaa aatcaccaag gcccctcttc cggggccttt tttattgccg
     2641 aggcttcaaa cacgcacctc ccctcctctt ttccacgctg tcatcttcct tttccctggc
     2701 tgcgactctg tccatcattc taacagcagc attcatcttc gcagcaaagg tcgccgcccg
     2761 tcagcaattc ctgaagttcc gccacatcat cgtcggttat ttccggctca ttgtggacct
     2821 gagcgtgggc gctcatgtcc ttaaccttgt cgaaaaaagg gtcctcttcg gatatgtaat
     2881 cgcagaccgg ccccatcata tctgcgtccc tgcatctgag aagaattgta gacataatgt
     2941 atgctccttt tcacctctca aagttccttc ctgtataaat tgtatcaacc cttccgtaga
     3001 tgcgtcaagg gcatcatccc gagtcggtcg ggaagcgacc aagcggtctg ctcgttgcct
     3061 gaaaaaaata ccctcccttg gaatgccaaa ggagggtatg ggattgctct tcagaaagta
     3121 tcagcgcgtc tacaccgtca tgatctcttt ttccttgtgc gcaaggagtt catcgatctt
     3181 gtggatgtag ctgtccgtcg tcttctggat gtcgtcctgc agtttcttgg tctcgtcctc
     3241 cgagacatgc gcgttcttgt ccttgctctt tttcttgatc tcgtcgttca catcccggcg
     3301 aatgttgcgc acggcgatcc gcgcgtcctc ggccagcttt ttcgcgtgct tcacgatctg
     3361 ctgccttcgc tcctcggtca gcgacgggat gttcaagcgg atgattttcc cgtcgttcgt
     3421 tggcgtgatg cctatgtccg acttcaagat cgccttttct atctcgccca gcatcttcgg
     3481 ctcccagggc tggatggtta tctggcgcgg atcaggcacg gccatgtttg ctacctggtt
     3541 cagcggagtt ggtgtgtcgt aatagtccac cacgatgccg tcgagcatgc ccaaggacgc
     3601 cctgccggtg cgcagagtgg cgaattcttt cctgagcgca tcgagcgcct tgttcatttt
     3661 gtcttcggct ttcttcttct gctcgctaag cattgttcct cccggaaacg agcgtcccga
     3721 tcgtttcgcc cattacgatt tttttgatgt tgcccgggac gttgaggttg aacacgatga
     3781 tgggaagatt gttgtccatg cacagggaaa tggccgttgc atccatgacg cgaaggtcct
     3841 tctgcagaac gtctatgtag gtgagccggt cgaacttggt agcagtcttg tccttcacgg
     3901 ggtcggcgct gtacacgccg tccaccttgg tggccttcat gatgacctcg gcgccgatct
     3961 ccat
//
LOCUS       DASSLD010000582         3502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_366301,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000582 DASSLD010000000
VERSION     DASSLD010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366301"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFK23_10495"
     CDS             complement(<1..438)
                     /locus_tag="VFK23_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7319555.1"
                     /translation="MDFLRTLGIEKINSGASTGTSWDTDTAGAVLEIRSPVDGKPIAS
                     VRLATMDAYDNVVKAAEAAFLQWRMVPAPKRGEIVRQIGQRLRMYKESLGMLVSYEMG
                     KSLQEGLGEVQEMIDICDFAVGQSRMLYGFTMHSERERHRMYEQ"
     gene            579..1496
                     /locus_tag="VFK23_10500"
     CDS             579..1496
                     /locus_tag="VFK23_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET7319556.1"
                     /translation="MTENRKIRKIFKSRPVIEGAGVHLRRVFGNSEVPIFDPFLLLDD
                     FRNTDPYKYIKGFPWHPHRGIETITYVLQGDVEHGDSMGNKGVITSGDMQWMTAGSGI
                     IHQEMPKGDDDGVMEGFQLWANLPKSRKMMDPRYRDIKSSQVPEVVTKGGAKVKVICG
                     TVDGRKGPVTDIVIDPEYLDVTLPARSEFVHKTTRGHTVFAYVIEGKGVFCNEKNPFA
                     YDALGENYFDMERDAYIHNGTAVLFDDGDQVTVAAQGEPVRFLLVSGKPIGEPVAWYG
                     PIVMNTEEELQTAFDEYRDGTFIKYRGGR"
     gene            1775..2203
                     /locus_tag="VFK23_10505"
     CDS             1775..2203
                     /locus_tag="VFK23_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET7319557.1"
                     /translation="MPDILHRVGINAKPEKVFKALSSIDGLRHWWIVGTKGNAKKGGV
                     IDFGFCKMKVVESAPRKLVHWTCMKGPKEWIGTHVIFELKWKDDQTFVIFRHAGWKEP
                     VEFMHHCSTKWAVFLLSLRDWMERAEGRPYPYDLKIHVGD"
     gene            2289..3068
                     /locus_tag="VFK23_10510"
     CDS             2289..3068
                     /locus_tag="VFK23_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HET7319558.1"
                     /translation="MMDPSGWVVAFSIGVFIAIVGCLEIGFRLGRRDAEKLSKAAHEG
                     IGPLEAAVFALLGLLLAFSFAGATSRFETRRQQIIREADAIGTAYLRLDLLPAANQPE
                     LRRLFRAYLDTRLRVYEKLADPEAAERELKHAADLQQVIWSHAVVATRRDPTSSATRL
                     LLPAINEMIDITTARTIALHTHLPDLIIALLIGASLLSGLIAGYAMAKRVSRSWLHMA
                     LYAFIVAITLYVVLDLEYPRFGLIRIESADQALTGLRDSIR"
     gene            3195..>3502
                     /locus_tag="VFK23_10515"
     CDS             3195..>3502
                     /locus_tag="VFK23_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET7319559.1"
                     /translation="MNKIARTLLLVVIFILSAGPVFAANKQDTQKAPPPGSAVVLIEP
                     EFTVNPARTALLIMDYQNDIVGMLPEDARTALVEQAGMVLKEARKAGLPIIYVVVRFR
                     P"
BASE COUNT          788 a          980 c         1020 g          714 t
ORIGIN      
        1 ttgctcgtac atccggtgcc gctcccgctc tgagtgcatg gtgaacccgt acagcatcct
       61 ggactggcca accgcgaagt cgcagatgtc gatcatctcc tggacctcgc caaggccctc
      121 ctggagggac ttgcccatct cataggacac gagcatgccc agggattctt tatacatcct
      181 gagcctctgg ccgatctgcc ggacgatctc gccgcgcttc ggggccggaa ccatccgcca
      241 ctggagaaag gcggcctcgg cggccttgac gacgttgtcg tatgcatcca tcgtcgcgag
      301 cctcacggag gcgatgggct tcccgtcgac cggggatcgg atttcaagca cggcgcccgc
      361 cgtgtccgtg tcccacgagg tcccggtcga agcgccggaa tttatctttt caatacctaa
      421 tgtcctgagg aaatccatgc acacctccgc acttctgttc cgaaggggga atgtattgcg
      481 aagtcttgtt cattccaatt atatcagcat gcatcaactg attgctgacc tgatatactg
      541 aataacagac gcgtgtgtgg atataaggag gaccggccat gacagagaac agaaaaatca
      601 gaaaaatatt taaaagcagg cccgtgatcg agggagcagg cgttcatttg aggcgcgtgt
      661 tcggaaacag cgaggtcccg atctttgatc ctttcctcct gctcgacgac tttcgaaaca
      721 ccgaccccta caaatatatc aagggtttcc cctggcaccc ccaccgcggc atcgaaacga
      781 tcacctatgt ccttcaggga gacgtcgagc acggcgacag catggggaac aagggcgtca
      841 tcacatccgg cgacatgcag tggatgaccg cagggagcgg catcatccat caggaaatgc
      901 ccaagggaga cgacgacggc gtgatggagg gcttccagct ctgggccaac cttccgaagt
      961 cccgcaagat gatggatccg cgctaccgcg acatcaagag ctctcaggtg cccgaggtcg
     1021 tgacgaaggg cggtgcgaag gtcaaggtca tctgcgggac cgtggacggg aggaagggtc
     1081 cggtcacgga catcgtcatc gacccggaat atctcgacgt cacgcttccc gcacgctccg
     1141 agttcgtcca taagaccacg cgcggccata ccgtattcgc ctacgtgatc gagggaaagg
     1201 gcgtcttctg caacgagaag aacccctttg cctacgacgc cctgggggaa aactatttcg
     1261 acatggagcg cgacgcttat atccacaacg gaacagcggt cctcttcgac gacggcgacc
     1321 aggtcacggt tgcggcgcag ggagagcccg tccgcttcct gctcgtatcg gggaaaccca
     1381 taggcgagcc cgtggcctgg tacggcccca tcgtgatgaa caccgaggag gaattgcaga
     1441 cagccttcga cgagtaccgc gacggcacgt tcatcaagta caggggaggt agataagcct
     1501 gttgcatggt tgaaacaggc cggtttgaca ttcaagcccc ttacctgtca ttctgagggc
     1561 gaagccgaag aatctgggat ttaatttgca cggggcaacc tcatatgaga atctctctct
     1621 gtctcccatc cagacccttc gcggagcctg tcccttcggc aggctcaggg caagctctga
     1681 gctagattct tcacttcact acgctccgtt cagaatgacg gcgaagggct cagggagata
     1741 aaagtgactc gttcaacaag aaaaggaggc tctcatgccg gacattcttc acagggttgg
     1801 aatcaacgca aagcctgaaa aggtattcaa ggccctttca tcgatcgacg gccttcgcca
     1861 ttggtggatc gtgggaacca aggggaacgc aaaaaaaggc ggggtgatcg atttcgggtt
     1921 ctgtaagatg aaggtggtgg aatccgcgcc ccggaagctc gtacattgga catgcatgaa
     1981 aggcccgaag gagtggatcg gaacgcatgt gatctttgaa ctgaaatgga aggatgacca
     2041 gacgtttgtc atcttcaggc acgcaggctg gaaggagccg gtggaattca tgcaccactg
     2101 cagcacgaag tgggcggttt tccttttaag cctgcgggac tggatggaga gggcggaggg
     2161 caggccatat ccttatgacc tcaaaataca tgtgggtgat tgagcccctg tgtccgacgt
     2221 gaaataaagt cagttcggtg cctccagagt gaggaatacc ttcatttcgg acaagaagga
     2281 cgggaagtat gatggaccct tcaggatggg tagtcgcgtt ttcaatcggg gtgttcatcg
     2341 caattgtcgg ctgtctcgaa atcggatttc gcctgggacg ccgcgatgcc gagaaacttt
     2401 cgaaggcggc gcatgaaggc atcgggcctc tcgaagcggc cgttttcgca ctgctcgggc
     2461 ttcttttggc gttcagcttt gccggcgcga cgtcccgctt cgagacacgg cgtcagcaga
     2521 ttatcaggga agccgatgcc atcggtacag catatctccg cctggacctg ctcccggcgg
     2581 ccaaccagcc ggagctgcgc cgcctgtttc gagcctacct cgacacgcgg cttcgcgtgt
     2641 atgaaaagct cgctgacccg gaagccgccg agcgggagct gaagcacgcg gcagacctgc
     2701 agcaggtaat ctggtcacat gcggtggtag cgacccggcg ggatcccacc tcttcggcga
     2761 cgaggctcct cctgcctgcg atcaacgaaa tgatcgatat caccaccgcg cgcacgatag
     2821 cattgcacac tcacctgccg gacctcatca tagcgctact catcggggcg tcactcttga
     2881 gcgggctgat agctggctac gcgatggcaa agcgggtaag ccgcagctgg ctgcacatgg
     2941 cgctctacgc cttcatcgtg gcaattacgc tttatgtagt gctcgatcta gaatatcccc
     3001 gtttcggcct gatccggatc gaatcggccg accaggccct taccgggttg cgtgattcta
     3061 tcagataggt gaagggaacc ggggtcaggt ccgataagcg tgaaggttga ccaggagatc
     3121 agatctctgc tcatcctttc cttacgacgg gaaaatccgt atcggcgatt aaggcacacg
     3181 gcaaggagga agtcatgaac aaaatagctc gaacattgct gctggttgta atcttcatcc
     3241 tgtccgcagg ccccgtcttc gctgccaata agcaggacac gcagaaagca cctccgccag
     3301 gaagcgcggt cgttctcatc gaaccggagt tcaccgttaa tccggcaaga acggctctgc
     3361 tcattatgga ctatcagaac gatatcgtgg gcatgctgcc cgaggatgcg cggacggccc
     3421 tggtggaaca agcgggcatg gtcctgaaag aggcccggaa ggccgggctt cccattattt
     3481 atgtcgttgt gcgctttcgt cc
//
LOCUS       DASSLD010000583         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1546003, whole genome shotgun sequence.
ACCESSION   DASSLD010000583 DASSLD010000000
VERSION     DASSLD010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546003"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VFK23_10520"
     CDS             <1..671
                     /locus_tag="VFK23_10520"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932546.1"
                     /note="catalyzes the reduction of adenosine
                     5'-phosphosulfate to AMP and sulfite; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylylsulfate reductase subunit alpha"
                     /protein_id="HET7319560.1"
                     /translation="NRQSTVKGLFMCGDICGASGHKFSSGSHAEGRIAAKSAIAFLLD
                     NPNYTPTIKETEEHLAAELYLPFEVFEKYKTYTTDTNTNPHYIRPKMLQTRLQKIADE
                     YFGGISTWYMTSKTMLEAGLSKLQVLKEDTSKMAASNLHELMRCWEQYHRILSLEAHA
                     RHILFREESRYPGYYYRGDFDLIDDNNWKCFTNSNYDATTDKYTLKKVPYVQLVEDAA
                     PVKH"
     gene            869..2161
                     /locus_tag="VFK23_10525"
     CDS             869..2161
                     /locus_tag="VFK23_10525"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HET7319561.1"
                     /translation="MRFCFTACKEVYLIMAENKVLVVGGGFSGLTVAVEAAEAGSDVI
                     IVEKNAYLGGRVTQLNKYFWKMCPPNCGLEIQYKRIKNNGQVKIYTLAEVEKISGSEG
                     NFDVTIKLQPRFVNEKCTGCNKCTEACEIERPNDFDFGMSKTKAAYLPHNQAFPLQYV
                     IDGSVCKGAACNKCAEACKYDAIHLDMKPETVQVKVGAVVMATGWNPYDATRMDNLGF
                     GKVQNVVTNMMMERLAAPNGPTAGKIVRPSDGKEAKNVAFVQCAGSRDENHLAHCSYI
                     CCLASIKQATYVRELYPDSKAKIFYIDMRTPGLFESRFYTKIKEDPNVVFLKGKVAKI
                     EQGANGDVLVTAEDIHGGGKVTETFDMVVLATGMQPTAASLKAAGVAVTDDGFVDQAA
                     LPKGIYATGTLKTPVDVARSAQDATGVVMKSIQSLRRK"
     gene            2164..>2363
                     /locus_tag="VFK23_10530"
     CDS             2164..>2363
                     /locus_tag="VFK23_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319562.1"
                     /translation="MEKKFGVYICRGCGIKDAMDLEKVAGAAKKVQNYKFHDVLCSPE
                     GVQLIKDDMAKEGVNTIIIAACS"
BASE COUNT          552 a          703 c          700 g          408 t
ORIGIN      
        1 acaaccgcca gtccacggtg aagggcctgt tcatgtgcgg cgacatctgc ggcgcatcgg
       61 gccacaagtt ctcctccggt tcgcacgcgg aaggccgcat cgccgccaag agcgcgatcg
      121 cgttcctcct cgacaacccg aactacacgc cgaccatcaa ggagacggag gagcacctgg
      181 cggccgagct ctacctgccg ttcgaggtct tcgagaagta caagacctac accacggaca
      241 cgaacaccaa cccgcactac atcagaccga agatgctcca gacccgtctg cagaagatcg
      301 ccgacgagta ctttggcggc atttcgacct ggtacatgac ctccaagacc atgctcgagg
      361 caggcctcag caagctccag gtgctgaagg aagacacctc caagatggcg gcttccaacc
      421 tgcatgaact gatgcgatgc tgggagcagt atcatcgcat cctctccctc gaggctcatg
      481 cgcgccacat cctgttccgt gaagagtcgc ggtatcccgg ctactactat cgcggcgact
      541 tcgatctgat cgacgacaac aactggaagt gtttcaccaa ctcgaactac gacgccacga
      601 ccgacaagta cacgctcaag aaggtcccgt acgtacagct cgttgaagat gcagctcctg
      661 tgaaacatta agaacatgct caactccggg gaggggaacc tccccggaga ttttattgta
      721 cattgcagat ccggtttttt tgacgtcatt cccgcctgtc tctgagcctg cccccgcatg
      781 tttgaagccg gggggaatcg cggtgtaacc gcatcgttcg ggagagacga ttccgggcaa
      841 gccggaatga catcaagacc gagctccaat tcggttctgt ttcactgcct gcaaggaggt
      901 ttacctaatc atggcggaaa ataaggtgtt ggttgtcggc ggaggcttca gcggcctcac
      961 ggtggcggtt gaggccgcgg aggcgggttc cgatgtgatc atcgtggaaa agaacgccta
     1021 tctcggcggc cgggtgaccc agctcaacaa gtacttctgg aagatgtgcc ccccgaactg
     1081 cgggcttgag atccagtaca agcggatcaa gaacaacggc caggtcaaga tctatacgct
     1141 cgccgaggtg gagaaaatct ccggctccga aggcaacttc gatgtgacca tcaagctaca
     1201 gccccgcttc gtgaacgaaa agtgcacggg ttgcaacaag tgcacggagg cctgcgagat
     1261 cgagcggccg aacgacttcg acttcggcat gagcaagacc aaggcggcct acctgccgca
     1321 caatcaggcg tttccgctcc agtacgtcat cgacggctcg gtctgcaagg gagcggcgtg
     1381 caacaagtgc gccgaggcct gcaagtacga cgcaatccac ctggacatga agcccgagac
     1441 cgttcaggtc aaggtcgggg ccgtggtcat ggccacaggc tggaacccct acgacgcgac
     1501 gaggatggac aacctcgggt tcggcaaggt ccagaacgtg gtgacgaaca tgatgatgga
     1561 gcggttggcg gcgcccaatg gaccgaccgc cggcaagatc gtacggccgt ctgacggcaa
     1621 ggaggcgaag aacgtcgcct tcgtgcagtg cgcgggctcg cgcgacgaga accatctggc
     1681 ccattgctcg tacatctgct gcctggcttc gatcaagcag gccacctacg tccgggagct
     1741 ctaccccgat tccaaggcaa agatcttcta catcgacatg cggaccccag gcctcttcga
     1801 gagccggttc tacaccaaga tcaaggaaga cccgaatgtc gtcttcctca agggcaaggt
     1861 cgccaagatc gaacagggcg caaacggcga cgtgctcgtg accgccgagg acattcacgg
     1921 cggcggcaaa gtcaccgaga ccttcgacat ggtcgtcctc gcgacgggca tgcagcccac
     1981 ggcagcgtcc ctcaaggcag ccggcgttgc cgtgaccgac gacgggttcg tggaccaggc
     2041 ggcgctcccc aagggcatct atgcgaccgg gacgctcaag acccctgttg atgtggcgcg
     2101 gtccgcgcag gatgccaccg gcgtggtgat gaaaagcatc cagagcctga ggaggaaata
     2161 aggatggaaa aaaaattcgg cgtatatatc tgcaggggat gcggcatcaa ggacgcgatg
     2221 gaccttgaga aggtcgccgg cgcggccaag aaggtccaga actacaagtt tcacgacgtc
     2281 ctgtgcagcc ccgagggcgt tcagctgata aaagacgata tggcgaagga aggcgtgaat
     2341 acgatcatca tcgccgcctg ctc
//
LOCUS       DASSLD010000584         5746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_282970,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000584 DASSLD010000000
VERSION     DASSLD010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282970"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="VFK23_10535"
     CDS             <1..1401
                     /locus_tag="VFK23_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319563.1"
                     /translation="TKGTFTFDTDATVVSSEEQDLGVDAQMVLMDALRILDERERDRA
                     AGKEVPSFVELYADVLPGERAGETEGEPPTITADDLGLEDLDRLEKKIPGPVSGMETF
                     DPVAIQRQKIMELLPGFSFEEQEAFFSFLRKAAGRKAAPDAAKQAGRAVVLFSGDTLI
                     SHSVMSLCNDEGIPVFATDEEKELDRMVSQCHLSGRMAVVVFDTPAGPEGGFSREHIT
                     GLRKLVSGKYPSVPLIQFASSQDRDFVLQSYYEGVRAVLPKPSKEDGRETYIQDTIRF
                     LDTFRSYVGSFQYGHDDDISVKKLKDGITSLSTITVPSDAALVVLTAVSEVFERGVTL
                     IVRASELIGERSIGIGTEKSMGPAPADRLKFQLSKPSVFRDVIEKGRAYYGESGDETL
                     RTFFAEIGKPASPAIVLLPLISNRKVVAVIYGDFGQKEASPVRLDMLEVLSLQAGIVL
                     EYALFRRQAAKAAQKS"
     gene            1623..2690
                     /locus_tag="VFK23_10540"
     CDS             1623..2690
                     /locus_tag="VFK23_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET7319564.1"
                     /translation="MDIAVFNQILHIAFEKRVSDIHFEVDNPPMFRARGHLIRAKLAS
                     LTKQDTEFVVNTVMAQHNRKPPEDLKEFDTSYTLANVGRFRVSIFRQRGNLGVVMRVI
                     PHQIGTFTELNLPSVLGRIAQAPNGLILVTGPTGNGKSTTLASMMRFINDNFDYNVIT
                     IEDPIEFLFTSNKSCIIQREVGIDTECFSDALRSAMRMNPDVIMVGEMRDLETIDACI
                     KAAETGHLVLATLHTHNTASSVNRMIGYFPPQAHENIRQRLAECLVALVSLRLVRGKK
                     EETILPVLEVMYATSSIKACIRDNHLDEIEQYIEKGRDDYGMQTMDQHLIELCRAGLI
                     TLDEAKRVSRSKDLERKLTYS"
     gene            complement(2814..3137)
                     /locus_tag="VFK23_10545"
     CDS             complement(2814..3137)
                     /locus_tag="VFK23_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319565.1"
                     /translation="MKEIATMEITENKLKEILTEQRTGFQHVVGIFKEDLESKINLIA
                     EQYQDIKATQKTQTEMVGALMEDVQIIKSDVQFLKVELKRKVDYDEFDALVKRVALLE
                     SKIRK"
     gene            3492..4160
                     /locus_tag="VFK23_10550"
     CDS             3492..4160
                     /locus_tag="VFK23_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7319566.1"
                     /translation="MNILEFKNVRKDYRLGETVVHALRGVDLSVGKGEFIAVLGASGS
                     GKTTLLNLAGAIDDPTDGEVVIDNRDISRLTDNQKAELRNGTIGYIFQTFNLVSVLTA
                     LENVMLSLQIRGGKMSKIRESAADRLDEVGLADYMHHRPDKLSGGQRQRVAIARALVT
                     RPMLILADEPTANLDHETAQSIIDLMRELNERQKATFVFSTHDQRLIDRVKRVVRIDD
                     GRIV"
     gene            4275..5522
                     /locus_tag="VFK23_10555"
     CDS             4275..5522
                     /locus_tag="VFK23_10555"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7319567.1"
                     /translation="MFMWLTISLRNIVKNGRRSLVTVLAIALGYAAINLFQGYVHSTY
                     QGLTNAAIHGEGLGHLTIFKKGYLDQGKLHPEKYQFSRDEAEKISSLVKSRPDVELVT
                     PRLSTAGIISNGKNSTIFISQGLVPADDKAIRGDLTKFTSFKGAYLTDSERSGVVVGS
                     ELAGMMNLKVGSDAVILSNTYSGMANALDVKVLGIYNTGASATNDKMVLMTYSHAQDL
                     MDFPGAERLVVLLKNGKSGKAAGTAASEIGKMLGDAGFGVEIKTWNELSVFYNQVKNL
                     FDMIFLFIFSIVLVIVVMSIINTMSMSVMERTREIGTMRALGLKRYGVKFLFATEGVL
                     LGILGSIAGGLIFFVVYSIIIATHPTYIPPASSNPVPLRVDLVWPALVRGVFFMAVLS
                     LGAAFVPARRSARMTIVDALGHI"
BASE COUNT         1312 a         1532 c         1643 g         1259 t
ORIGIN      
        1 acgaagggta cgtttacgtt cgacacggat gcgaccgtcg tttcctcgga agagcaggat
       61 ctgggagtcg atgcccagat ggttttgatg gatgcgctgc gcatactgga tgagcgggaa
      121 cgtgaccgcg cggccggaaa agaagttcct tcctttgtgg agctctatgc cgatgttcta
      181 cccggggaaa gagccggtga aacagaaggg gagcctccga ctattaccgc ggatgatctc
      241 ggacttgaag acctcgaccg gcttgagaag aaaatacccg ggcccgtttc cggaatggaa
      301 acctttgatc cggtcgcaat ccagcgccag aaaatcatgg aattgctccc gggtttttcc
      361 ttcgaggagc aggaggcgtt cttttctttt ctgaggaaag ccgcgggccg caaggccgcg
      421 cctgacgcgg cgaagcaggc gggcagggcc gtcgtcctgt tcagcggtga tacgctgatc
      481 agtcattcag tgatgtctct ctgtaacgac gaggggatac cggtatttgc gactgacgaa
      541 gaaaaagagc tcgaccggat ggtatcgcag tgccacctct ccgggagaat ggccgtcgtt
      601 gtctttgaca cacccgcggg cccggagggc gggttttccc gggaacacat cacagggctg
      661 aggaagctgg tgagcggaaa gtacccttcg gttcccctga tccaattcgc ctcttcccaa
      721 gaccgtgatt tcgtccttca atcgtactat gagggcgtga gggccgttct tcccaaaccg
      781 tcgaaagagg acggtcgtga aacgtacatc caggacacga taaggttcct cgacaccttc
      841 aggtcgtatg tcgggagttt tcagtacggc cacgatgatg atatctccgt caagaaactg
      901 aaagacggca tcacgtctct cagcacgata acggttcctt ccgatgcggc gctcgtggtt
      961 ctcaccgccg tctccgaggt gttcgaacgc ggggtcacgc tcatcgtccg tgcttcggaa
     1021 ttgatcggcg aacggtccat agggatcggt accgaaaaaa gcatggggcc ggctccggcg
     1081 gaccggctga agttccagct ttcaaagcct tcggttttcc gggatgtgat cgaaaaaggg
     1141 cgggcctatt acggagagag cggcgatgaa acgctcagga cgttctttgc ggaaataggc
     1201 aaaccggcca gtcccgccat cgttctgctg cccttgataa gcaatcgaaa ggtcgtagcg
     1261 gtgatctacg gggatttcgg gcagaaagag gcgtctcccg tgcggctcga tatgctcgaa
     1321 gtcctctccc tgcaagcggg cattgtcctg gaatatgccc ttttccgcag acaggcggcg
     1381 aaagcggctc aaaaatcata gatacctgac ccggacagac cggaaccgat agaacaaaag
     1441 cacctcgcgc aggggcgcgg agtcgcagag aacggcgaaa agttcattta agtctttgat
     1501 ttcctctgcg ccacagcgtc tcagcgcgat tacgattttg gttttgattt agcctatttg
     1561 aaatcccgga gttgattttc attggtgact ggccaagaaa aatcgcggag gtacggcgcg
     1621 ccatggacat tgcggtattc aatcagattc ttcacattgc tttcgaaaaa agggtctcgg
     1681 atatccattt tgaggtcgac aacccgccga tgtttcgtgc acgcggccat ctgattcgcg
     1741 ccaagctcgc gagcctgacg aagcaagaca ccgaattcgt ggtaaacacg gtcatggcgc
     1801 agcataaccg gaagccgccc gaagacttga aggaattcga tacgtcctat actctggcga
     1861 acgtcggacg cttccgggtg agcatcttcc gtcagcgcgg caatctcggc gttgtcatgc
     1921 gcgttatccc tcaccagata ggtacgttca cggagttgaa tcttccttcg gtgctcggca
     1981 gaattgcgca ggccccgaac ggcctgattc tcgtcaccgg gccgaccggc aacggtaaat
     2041 caacgacgct cgcttcgatg atgaggttta tcaacgataa tttcgattac aatgtcatta
     2101 cgatcgagga cccgatcgag tttctgttca cttcgaacaa aagctgtatt atccagaggg
     2161 aagtgggcat cgatacggaa tgtttcagcg atgcgttacg ctctgcaatg cgtatgaatc
     2221 cggacgtcat catggtcggg gaaatgcgcg atctcgagac catagacgcc tgtataaagg
     2281 cagcggagac ggggcatctg gtgttggcca cgcttcatac ccataatact gcttcttccg
     2341 tcaaccgcat gatcgggtat ttcccgcccc aggcgcacga gaatatcagg caaaggctgg
     2401 cggagtgcct tgtcgcgttg gtctcgctga ggcttgtaag agggaaaaaa gaggaaacta
     2461 ttcttcccgt ccttgaagtg atgtatgcaa cgtctagcat caaggcatgc atcagggaca
     2521 atcatctcga tgagatcgag cagtacatcg aaaagggacg cgacgactac ggcatgcaga
     2581 cgatggacca gcatctcatc gagctctgca gggccggact cataactctc gatgaagcaa
     2641 agcgcgtctc ccgctcgaaa gacctcgagc ggaagctgac gtacagttga gacacgcgat
     2701 ataggctgaa ccgctgagac gcagaggcgc agagcaaggc ttctgaggaa gacatcgagt
     2761 aatcactggc gtgttatggt ttgaacttca ccaggagaat gttttgaggc tgtttatttc
     2821 ctgatttttg attcgagcag agcaacccgt tttacgagcg cgtcgaattc gtcgtaatcg
     2881 acttttcttt tcagctcgac ttttaaaaat tgaacatcgg atttgatgat ttgtacgtct
     2941 tccatcaaag ccccgaccat ttcggtttga gtcttctgcg tagccttgat atcctggtat
     3001 tgttcagcga tgagattgat ctttgattcg aggtcttcct tgaagatacc gacaacatgc
     3061 tgaaatccag ttcgctgttc agtaagaatt tcttttaatt tattctcggt tatctccatg
     3121 gttgcaatct ccttcattat ggatgcctgg atattatacg tggagcccgc attttgtcaa
     3181 gctatctctc ccgacaaaat attccaacta cgcgctcgaa agagtccggg cgatgaacac
     3241 gcccggcctt gagtgcgaca ctcttccggc gcttcgcagc cagggaattc ccgctctcct
     3301 cccccgcaat cccagctgaa acgcctgatc agttcatcct ccccccaata ccattcatcc
     3361 ctcaatttct cccgttcgtc gcacatttct tgtcattcgt ccttgcttgg ggtatcgttg
     3421 ggacagaaag aattcctctc acccctgcac tctctcgcag ggagataggg atgagatgac
     3481 ggaggctagc gatgaatatc ctcgaattca agaacgtcag gaaagactac cggctcggcg
     3541 agaccgtggt gcacgccctg cggggggtgg acctgtccgt cggaaagggc gagttcatcg
     3601 ccgtgctcgg cgcgtcgggg agcgggaaga ccacgctcct gaacctggcg ggcgccatcg
     3661 atgatccgac ggacggggag gtcgttatcg acaaccgtga catttcccgt ctcaccgaca
     3721 accagaaggc agagctccgg aatgggacca tcggttacat cttccagacc tttaacctcg
     3781 tgtccgtcct gaccgcgctc gagaatgtga tgctgtcgct ccagatcagg gggggcaaaa
     3841 tgtcgaagat ccgcgagtcg gcggcggacc ggctcgacga ggtggggctg gcggactaca
     3901 tgcaccatcg gccggacaag ctctccggcg gccagcgtca gcgagtggcc atcgcgcggg
     3961 cgctcgtgac caggccgatg ctcatcctgg ccgatgagcc cacggcaaac ctcgaccacg
     4021 aaaccgcgca gtcgatcatc gatctcatga gggaattgaa cgagcggcaa aaggctacgt
     4081 tcgtcttctc gacccacgac cagcggctca tcgacagggt gaaacgggtg gtgaggatcg
     4141 acgacgggag aattgtatag caggaaggtt ggaaggtagg aacgtgggag ggtgggaaaa
     4201 aacgaaagtc agagataaaa catcccactt tctcaccatc ccaacctctt accctcgatc
     4261 cgtaggaggt tatcatgttc atgtggctca ccatatcgct cagaaatatc gtcaagaacg
     4321 gcaggcgcag cctggtgacg gtccttgcca ttgcactcgg ctacgcggca atcaatctct
     4381 tccagggata cgttcacagc acctaccagg gcctgacgaa cgccgcgatc cacggagagg
     4441 ggctggggca cctgaccatc ttcaagaagg gatatctcga ccaggggaag ctccatccgg
     4501 agaaatacca gttttcgcga gacgaggccg agaaaatttc ctcccttgtc aaaagcaggc
     4561 ccgacgtgga actcgtgacg ccgaggcttt cgactgccgg catcatctcg aacggtaaga
     4621 actccacgat cttcatctct caggggctgg ttcctgccga cgacaaggcc atcaggggag
     4681 acctgacgaa attcacctca ttcaaaggcg cctatctcac tgacagcgag cggagcggcg
     4741 tcgtcgtggg gtctgaactt gccgggatga tgaacctcaa agtcggcagc gatgcagtga
     4801 tcctgagcaa tacctattcg ggcatggcga acgcgctgga cgtgaaggtc ctggggatct
     4861 acaacaccgg ggcttctgcc acgaacgaca agatggtgct catgacctat agtcacgcac
     4921 aggacctcat ggacttcccc ggggccgagc ggcttgtcgt gcttctgaaa aacggaaaga
     4981 gcggcaaggc cgccgggact gccgcgtctg aaatcgggaa gatgctcggc gatgcgggct
     5041 tcggcgtgga gatcaagacc tggaacgagc tttcggtgtt ctataatcaa gtaaagaacc
     5101 tcttcgacat gatctttctg ttcatttttt ccatcgtgct ggtgatcgtg gtcatgagca
     5161 tcataaacac catgagcatg tcggtgatgg agcggacccg ggagatcggc accatgcgcg
     5221 cgctcgggct caagcgctac ggagtaaagt tcctgttcgc caccgaaggc gtgctcctgg
     5281 gcatcctggg cagcatcgcg ggagggctga tctttttcgt ggtctacagt attatcatcg
     5341 cgacccaccc gacctatatt ccgcccgctt cgtcgaaccc ggtgccgctc agggtggacc
     5401 tggtatggcc ggcgctcgtc aggggagtgt tcttcatggc ggttctttcc ctgggcgcgg
     5461 cgttcgttcc cgcgaggcgg tcggcaagga tgacgatagt tgatgcgctg ggacacatct
     5521 aacggcaatg cggaatgcgg aattcggaat agaatacgag gaaagcaatt atccaggagg
     5581 tagcgatgaa gcgcatttcg attatcacaa tcggactgat ggtgctcgtc ttcggtttcg
     5641 ggaaaaacgg tcgagcgggc gatccatcag ttatagacat cctcaagcac tccgaccggg
     5701 ctcgggggaa cgtgggcggc atcatatggg acattttgat cagcac
//
LOCUS       DASSLD010000585         4708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1545371, whole genome shotgun sequence.
ACCESSION   DASSLD010000585 DASSLD010000000
VERSION     DASSLD010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545371"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VFK23_10560"
     CDS             <1..732
                     /locus_tag="VFK23_10560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET7319568.1"
                     /translation="PVQLVKAVEPGRISFHLLHNKDYSRLTRRMFCPEENTMVSPDET
                     IRGYEIVPGKYILLSDEELESVSPERSRTIEIVEFIDLKDVDPIYFDHPYYLVPSKGG
                     EKAYQLLVEVMRRTNKAGLAKFVLAEREYLVAVTSREGALALTTLHYHNEIVPDEDIA
                     PKKGKIEAEEESRIKESIEQMIEDFDPGKYADGRRKKLLDLLAAKAKEKAPVEAPAVE
                     EEEGEGPPDLVTALEESMRKVKENR"
     gene            729..1634
                     /gene="ligD"
                     /locus_tag="VFK23_10565"
     CDS             729..1634
                     /gene="ligD"
                     /locus_tag="VFK23_10565"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET7319569.1"
                     /translation="MSRTFVEIEGRRLPLSNLEKDLYPSYGFTKAGILEYYRAMAPYI
                     LPHLKDRALTFKRYPEGVEKDFFFEKRCPGHRPVWVKTAEVPQKDEEPITFCLVNDLE
                     TLIWVENLASLELHVPLARAGSPETADAMVFDLDPGDPANILDCGRVALILRDLLVHM
                     GLSSYVKTSGQKGLHVLVPLNRTDTTFEDTKTFSRAAAEIMQKHYSGLVTAKMSKKER
                     QGKVFINWSQNDSKKSMICVYSLRARERPFVSFPLEWKELEGLLELGDPERLQVLYSE
                     ALRRVEEKGDLFRDVLVKEQKLPHI"
     gene            1760..3391
                     /gene="ligD"
                     /locus_tag="VFK23_10570"
     CDS             1760..3391
                     /gene="ligD"
                     /locus_tag="VFK23_10570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET7319570.1"
                     /translation="MGLKEYTSKRQFEKTPEPKPGLQAREGRLMFVVHKHAARALHYD
                     LRLELDGVLKSWAVPRGPSLDPSLKRLAVMVEDHPLEYKDFEGVIPEGSYGAGSVIIW
                     DRGFYHHPSAQDEKTGEKLLREGLNKGNMKFVLEGEKLHGEFALVKTGKGDGKSWLLL
                     KKKDRSAAKDDILKENRSIVSQKTLEEMLETDTGKSFKRKKVSQIRLHEAMESEDLKG
                     APLRPMPKDVKPMLATAIREPFDNPDWVFEVKWDGYRAVAEIRDGGVSLYSRNLISFN
                     RKFSPVVDSLRKIGTDAVLDGEIVVVDEQGRPDFQALQHYPDSQGGHLLYYVFDLLHF
                     QGHDLTGLPLSRRKELLKKILPSDPRIRYSDHVAKEGVLFYRVAGEKGLEGIIAKHAN
                     SAYETGRRSRQWLKIKTRLTQEAVIAGFTEAGGGRKYFSALVLGLFEEGEFRYIGHVG
                     GGFSAKDLKEVREKLDPLVRKECPFAVAPETNAPVTWVTPELVCEVALSGWTEDAVMR
                     QPVFLRLREDKAAREVVRESELFHRGGAETQRKEH"
     gene            3475..4215
                     /locus_tag="VFK23_10575"
     CDS             3475..4215
                     /locus_tag="VFK23_10575"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET7319571.1"
                     /translation="MKIYAGTSGFAYKEWKGKFYPEKIPPKEMLHFYSERLGAVEINN
                     TFYRMPRESVLKSWAEQVSGGFIFALKAPQVITHIKQLRNVFEETEYLFKALSVLGGK
                     LGPVLFQFPKSFRANVLALEDFLALIPGDTDCAFDFRSPTWLDAGMPDLLRKKGCSLC
                     MEDTDENPVNGIIGTSTWGYLRLRRSEYTEDGLSEWAERILSQKWERAFVFFKHEDGA
                     RGAEMALRFQELIGARGKSRTETMKKAG"
     gene            4488..>4708
                     /locus_tag="VFK23_10580"
     CDS             4488..>4708
                     /locus_tag="VFK23_10580"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HET7319572.1"
                     /translation="MDVLEMALKTETDAIAFYTEAARKTSFPAGKRMFETITEDEKRH
                     LDMIRQVVKGLHITHKDVSPMKNVKTVFEA"
BASE COUNT         1146 a         1199 c         1429 g          934 t
ORIGIN      
        1 ccagtgcagt tggtcaaggc cgtcgagcct ggccgcatct cgtttcacct gctccacaac
       61 aaagattact cccgcctcac gcggagaatg ttctgccctg aagaaaatac gatggtctcc
      121 cctgacgaaa caatcagagg atatgagatc gtaccgggca aatacatcct cctgtccgac
      181 gaggagctgg aatccgtatc tcccgagagg agccgcacga tcgagatcgt cgagttcatc
      241 gatctgaagg atgtggaccc gatctatttt gaccaccctt attatctcgt tccttccaaa
      301 gggggtgaaa aggcatatca gctcctggtc gaggtcatgc gccggacgaa caaggccggg
      361 ctcgcgaagt tcgtgctggc cgagcgcgag tatctggttg cggtcacaag cagggagggt
      421 gcgctggccc tgacaacgct tcactaccac aacgagatag ttcccgacga ggacattgct
      481 ccgaagaagg gaaagatcga agcggaagag gaaagccgga taaaggaaag catcgagcag
      541 atgatcgaag atttcgatcc gggaaaatac gcggacggtc gcaggaagaa gctcctggat
      601 ctcctggctg cgaaggcgaa ggaaaaggcg ccggttgagg cacccgcggt ggaggaggaa
      661 gagggagaag gtccgcccga cctggtgacc gcgctggagg agagcatgcg gaaagtgaag
      721 gagaaccggt gagcagaaca ttcgtcgaga tcgaggggag gagactcccg ctgtcgaacc
      781 ttgaaaagga cctgtatccg tcctacggtt ttacgaaggc tggcattctg gaatactacc
      841 gggcgatggc gccgtatatc ctgccccact tgaaagaccg tgcattgacc ttcaagcgct
      901 atccggaagg agtggaaaaa gacttctttt tcgagaagcg ctgtccggga caccggccgg
      961 tgtgggtgaa aacggctgaa gtcccccaga aggacgaaga gccgattacc ttctgcctgg
     1021 tcaatgatct cgaaacgctg atatgggtgg agaaccttgc gtcgcttgag ctccatgtgc
     1081 ctctggccag ggcgggctcg ccggagactg ccgatgccat ggtcttcgac ctcgatccgg
     1141 gcgaccccgc aaacattctc gactgcggcc gggtggcgct gatactccgg gacctgctcg
     1201 ttcacatggg gctttcgagc tatgtcaaga cctcgggaca gaaaggtctc catgttttgg
     1261 tgcctttgaa ccgcacggat acgacctttg aggacacgaa gacattttca agggctgcgg
     1321 cggaaatcat gcagaaacat tattccggcc tggtgaccgc gaaaatgtcg aagaaggagc
     1381 gacagggaaa ggttttcatc aactggtccc agaacgactc gaagaaatcg atgatctgcg
     1441 tctattccct gcgtgcccgg gaacggccgt tcgtatcgtt tccccttgaa tggaaggaac
     1501 tggagggcct cctggagctc ggcgatccgg aaaggctgca ggtcctgtac tctgaggccc
     1561 ttcgcagggt agaggaaaag ggagaccttt ttcgcgacgt gctcgtgaag gagcagaagc
     1621 tgccgcatat ataaacttgc cacagagaac acagagggta cagggaaaaa gctaatagtt
     1681 taaagtcagc tgaatttttg acttcttttc tctgtgttct cggtgacctc tgtggctaaa
     1741 tatctttttt aggttttgta tgggattaaa ggaatataca tcgaaacggc aattcgagaa
     1801 aacgccggaa cccaaaccgg gacttcaggc tcgggagggc agacttatgt ttgtcgtcca
     1861 taagcacgcg gcccgggcct tgcactatga tctcaggctc gagctcgacg gggtgctcaa
     1921 gagctgggcc gtgccgcggg ggccttcgct cgatccctcc ttgaagaggc tggctgttat
     1981 ggtcgaagac cacccgcttg agtacaagga tttcgagggt gtcatcccgg aaggcagtta
     2041 tggggcgggc agcgtgatca tctgggacag gggcttctat catcatcctt cggcgcagga
     2101 cgaaaagacc ggcgaaaagc tcctccggga gggactgaac aaggggaata tgaagttcgt
     2161 cctcgaaggg gagaagcttc acggcgagtt cgccctggta aaaacgggaa aaggggacgg
     2221 gaagtcgtgg ctcctcctga agaaaaaaga ccgaagcgcg gccaaagacg acatcctgaa
     2281 ggagaatcgt tccatcgtgt cccagaaaac actggaggag atgctcgaaa ccgatacggg
     2341 aaaatccttc aaacgaaaga aagtaagcca gatccgcctt catgaggcga tggaaagcga
     2401 agacttgaag ggtgcgccgc tcaggcctat gccgaaggac gtaaagccga tgctcgcgac
     2461 cgctatcagg gagccctttg ataaccccga ttgggtcttc gaggtgaagt gggacggata
     2521 ccgggccgtt gcggagatcc gggacggagg cgtttccctg tattcccgga acctgatatc
     2581 attcaacagg aagttttctc ctgttgtcga ctcccttcgg aaaatcggca ctgacgccgt
     2641 cctggacgga gaaatcgtcg tcgtagatga gcagggtcgg cccgatttcc aggcgctgca
     2701 gcattacccg gactcccaag gcggccatct gctctactac gtcttcgatc ttctccactt
     2761 ccagggacac gatctgacgg gtctgcccct ctcccgacgg aaagaactcc tgaaaaaaat
     2821 tcttccgtcc gacccgagaa tacggtatag cgatcacgtc gccaaggaag gagtcctgtt
     2881 ttaccgggtt gccggggaaa aagggttgga aggcataatc gccaaacatg cgaacagtgc
     2941 gtacgaaaca ggcagaagga gcaggcagtg gctcaagata aagacgcggc ttacgcagga
     3001 ggccgtgatc gcgggattca cggaagcggg aggaggcaga aagtatttca gcgcccttgt
     3061 tctcggtctc ttcgaggagg gcgagttcag gtacatcgga catgtggggg gcggattctc
     3121 ggcaaaagat ttgaaggaag tgcgcgaaaa actggatcct ctcgtccgga aagaatgccc
     3181 ctttgccgtg gcgccggaga ccaacgcccc cgtcacctgg gtcacgccgg agctggtctg
     3241 cgaggttgcg ttgtccggct ggactgaaga cgccgtcatg aggcagccgg ttttcctgcg
     3301 gctgcgcgag gacaaggccg cccgcgaggt ggtgcgggaa agcgaactgt ttcaccgcgg
     3361 aggcgctgag acgcagagaa aagaacatta aagaattggc tttctctccg tcctccgtga
     3421 ctctgcggta agacaagcag tgatgaatgt cattgatgct ggaggaaata aatcatgaaa
     3481 atctacgccg gtacaagcgg atttgcctat aaggagtgga aagggaaatt ctatcccgaa
     3541 aagatcccgc ccaaggaaat gctgcacttc tattcggagc gactgggcgc ggtggagatt
     3601 aacaacacct tttatcgcat gccccgggag agcgtgctca agtcctgggc ggaacaggtt
     3661 tccggcggtt tcattttcgc gctcaaggcg cctcaggtca taacgcacat aaagcagctt
     3721 cggaacgtgt tcgaggagac cgagtacctc ttcaaggcac tgtcggtcct gggcgggaag
     3781 ctggggccgg tcctttttca gtttcccaaa agtttccgcg cgaacgtgct ggcgctggaa
     3841 gattttctcg ctttgattcc cggcgatacg gactgcgctt tcgatttccg cagccccacg
     3901 tggctcgatg ccggaatgcc ggatctcctt cgtaaaaagg gatgcagcct ctgtatggag
     3961 gatactgatg agaatccggt aaacgggatc atcggtacgt caacctgggg ataccttcgg
     4021 ctgcgccgtt ccgagtacac ggaagacggc ctgtcagaat gggcggaaag gatcctttcg
     4081 cagaaatggg agcgggcttt cgttttcttc aagcacgagg acggggccag gggggccgaa
     4141 atggcgctcc gttttcagga actcatcgga gcaaggggga agagcaggac ggagacgatg
     4201 aagaaggccg gttaaagcga gggacgccgg tcgatgcacc ttgtttttat ctgctgtctc
     4261 tcggtaaatt acccgagtgg ctcgaagcgt gaagtacgcc gaacttgaac gccatccgcg
     4321 cgttttcccg tcgtgactca acatgaccgt gaggggaccc gagggccatc gggccgtaag
     4381 acgctcaaga tgctcccgcg tcatctcata ccttaccgcc gtgatataat tagtaccacg
     4441 atctaaccga tcgcatcacc cattgaacga taacggagga agagcttatg gacgtgctcg
     4501 aaatggccct gaaaacagag acggacgcca tagcgttcta tacggaagcc gcccggaaga
     4561 catcattccc cgcggggaag aggatgtttg agacgatcac cgaggatgaa aaacgtcacc
     4621 tcgacatgat acggcaggtc gtgaaaggcc tccacatcac gcacaaggac gtgagcccga
     4681 tgaagaacgt gaaaaccgtg tttgaggc
//
LOCUS       DASSLD010000586         2179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1425941, whole genome shotgun sequence.
ACCESSION   DASSLD010000586 DASSLD010000000
VERSION     DASSLD010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425941"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..1126
                     /locus_tag="VFK23_10585"
     CDS             335..1126
                     /locus_tag="VFK23_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7319573.1"
                     /translation="MLSIMTVKAISLQSGSSGNCIYVEAGGVRLLFDAGITGVQTKER
                     LAAAGRDVRDVDAVIISHDHADHCSHAGVLQRKFGLPVYMTPATYAAAAARCPLGKMK
                     DVRHFRSCDKLRFGDVVVHAVPTPHDGADGSVFVIEAGGKRLGVMTDLGHVFKDLIGL
                     VSSLDAVFLESNYDPDMLSHGPYPVYLKQRIKGPRGHISNIEAAEVLSRAGAGSRLQW
                     ACLAHLSEQNNHPEVALRTHREVVADTLTLYVADRYKASDVFTVV"
     gene            complement(1316..>2179)
                     /locus_tag="VFK23_10590"
     CDS             complement(1316..>2179)
                     /locus_tag="VFK23_10590"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET7319574.1"
                     /translation="RQGVDRKEAGRILAEAYAQMFFSDGLFHGDPHPGNIFVRPGPEV
                     ILVDFGMVDRLSTPKREGLRRAFAAIIERDALGLVRSLVDMGFMPLTQDIKPLVQFVE
                     KMFVKYRDMSASEFKAMDIDEIDRDITEALQLTPSIQIPNDFILFGRVIGMLNGLGSR
                     LDPDTNIIEIAAPFARRFISEELTAAGVLKQASLCARSAVQLPKMLADFLVTTGRGET
                     RVEITSRDVVEALTRIYRIGKGFILALLTVGAGTAAVAFRISRFDTESAWSAGIGAVL
                     FLWLVLVLRRK"
BASE COUNT          455 a          667 c          649 g          408 t
ORIGIN      
        1 cacgaggacc agccgggaga ggcccatgtt catcatcgac cgcgcaaccg cgccgatgtt
       61 ggccggcgat tttgtgtcca cgaggataat ggtgacgttg tcgagaagtg aatggttctg
      121 catggacaga tgaaaacgta aagtttctaa aagtcggaga ctcaccgcag aggcgctgag
      181 acgcagagaa aatcacggca agactcgcgt cagaaaaaaa aagctgaaat cctccgcgcc
      241 tccgcgtctc tgcggttcaa ggattatagt tttgtttcat cggtcggcgg ctttgcctcg
      301 ttacttattg acaaacccct ccgggactgc tacagtgctc tccatcatga ccgtgaaggc
      361 catttccctt cagtccggca gcagcggcaa ctgcatctat gtcgaggccg gcggcgtcag
      421 gctgctgttc gatgccggca tcaccggcgt tcagacgaaa gaacggcttg cagcggccgg
      481 cagggacgtg cgcgatgtgg atgccgtgat catatcccac gatcatgcgg accactgcag
      541 ccatgcgggc gtactgcagc gaaaattcgg ccttcccgtg tacatgacgc ctgccaccta
      601 tgcagcggcg gccgcacggt gcccgctcgg aaagatgaag gacgtccggc atttccgctc
      661 atgcgacaag ctccggtttg gagacgtcgt cgttcacgcg gtgcccacgc cccatgacgg
      721 cgcggacggc tcggttttcg tgatcgaggc gggcgggaag aggctggggg tcatgaccga
      781 cctgggccat gtgttcaagg acctgatcgg actggtttcc tccctcgacg ccgtcttcct
      841 tgaaagcaac tacgacccgg acatgctctc gcacggcccc tatccagtct acctcaagca
      901 acgaatcaaa ggtccgcggg gccatatctc gaacatcgaa gccgccgagg tgctctcccg
      961 cgccggagcg ggaagccgcc tccagtgggc ctgcctcgcc catctctcgg aacagaacaa
     1021 tcaccccgag gtggccctcc ggacccaccg cgaagtcgtg gccgacacgc tcaccctcta
     1081 cgtcgccgac cgctacaagg cgtcggatgt attcacggtc gtgtaactcc ccaccctgtc
     1141 tcaaagcaac agtggacgca gataaaatcc gatgaacgca gatcaaaagc catattcaga
     1201 attaacgaca gatgacttct ctttggtgag agaacattca gtttcgtaaa atggttcagt
     1261 ggggtttatc cgcgtaaatc tggcgccatc cgcgtcccat gccgtttcga aataatcact
     1321 tcctcctgag caccaacacc agccacaaga acagtaccgc gcctatcccc gcggaccagg
     1381 cgctctccgt atcgaaacgg ctgatccgga acgcaaccgc cgcggtgccg gcgcctacgg
     1441 tgaggagggc gagaatgaac cctttgccga tgcgataaat cctcgtcaag gcttccacga
     1501 cgtcccttga agtgatctcc acgcgggtct ccccccggcc cgtggtcacc aggaaatcgg
     1561 ccagcatctt cggcagctgc acggccgacc gggcgcagag ggaggcctgc ttgagcacgc
     1621 cggcggcggt cagctcctcg gagatgaaac ggcgcgcgaa gggcgcggcg atctcaatga
     1681 tattggtgtc gggatcgagg cgggagccga ggccgttcag catgccgatg accctgccga
     1741 agaggatgaa gtcgtttggt atctggatcg acggggtgag ctgaagcgcc tcggtgatgt
     1801 cgcggtcgat ctcgtcgatg tccatggcct tgaactcgct ggccgacata tcgcggtact
     1861 tgacgaacat cttttcgacg aactgcacga gcggtttgat gtcctgggtg agcggcatga
     1921 agcccatgtc cacgagcgaa cgcaccagtc ccagtgcgtc gcgttcgatg atcgcggcaa
     1981 aggcacggcg gagaccttcc cgtttgggcg tcgacagccg gtcgaccatg ccgaagtcca
     2041 caaggatcac ctcgggcccc ggcctgacga agatgtttcc ggggtgcggg tcgccgtgga
     2101 acaggccgtc ggagaagaac atctgggcat aggcctcggc aaggatgcgg cccgcctcct
     2161 tccggtcgac accctgccg
//
LOCUS       DASSLD010000587         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_534818,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000587 DASSLD010000000
VERSION     DASSLD010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534818"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VFK23_10595"
     CDS             complement(<1..496)
                     /locus_tag="VFK23_10595"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HET7319575.1"
                     /translation="MLHSSVISFGTAILMGLGLGYVLQRGRFCLNSAFRDIIFIQDLT
                     LFRAYLLSVVVAIIGSNLIEDLGFLQAINPDTGALEATMLMRQTFVPVANILGGFLFG
                     LGIVLAGGCASGIVYRIGEGQLSALTAIIGFFFGAVMTADGLLSPVNNYLKNFRVEIN
                     GTTSP"
     gene            complement(872..1387)
                     /locus_tag="VFK23_10600"
     CDS             complement(872..1387)
                     /locus_tag="VFK23_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7319576.1"
                     /translation="MKKVLIAVDETEGSTSVLSVFRNMVRPPESVVLVHVQQLEGKSM
                     IIDMLGEAEKSTLRESLKGTAHKEALDRRSEKILSFYKKELDSGGLVNVKTVVRDGAP
                     SEEILKVAQEEGVDLIITGYNGKTLMQRLISGSVSKDVEKGAPVPVLVAKNPTEAEKY
                     AWSAAAAVNCK"
     gene            complement(1444..2031)
                     /locus_tag="VFK23_10605"
     CDS             complement(1444..2031)
                     /locus_tag="VFK23_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7319577.1"
                     /translation="MNKKIMIAVDDTKGSKNVLSVFHNFVQQPADVTLLHVERLQGRS
                     LMIDMLGDAEMSTLKEMMKGTDYKAALDKRSDKILAFYSKELSSNASFPLKTIKREGI
                     PAEEILKAADEEKADMIILGQSGKRGFDRFITGKVAKYVEKNAKVPVLVAKPPLMCEE
                     AYTKKDAWAAVSVTTAIMATLFILGVILQKGLLIH"
     gene            2265..2525
                     /locus_tag="VFK23_10610"
     CDS             2265..2525
                     /locus_tag="VFK23_10610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7319578.1"
                     /translation="MIEKFCKDPKFLMSVVETMRDGLMIVDKDGTILLFNRAAEEITG
                     YCKEDVIGQQCSILDSDTCVVLTEGGKQRSCALFKSGVIENR"
     gene            2556..>3170
                     /locus_tag="VFK23_10615"
     CDS             2556..>3170
                     /locus_tag="VFK23_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HET7319579.1"
                     /translation="MHLLKNATILNDENGEIVGAVESMTDITSLYMKELELEELKQEL
                     RQEYWFMGLLGKSAPMQKLFEQIRNAAQSEAPVLICGESGTGKNLVAKAIHALSRRKE
                     GPFVGVNCGAFNDYLLESELFGHRKGSFTGAVSDRTGRFEAAHNGSIFLDEIGDMPKV
                     MQVKILRVLEEKVVELVGDHRPIPVDIRLISATNKDLDTLVKSDL"
BASE COUNT          774 a          852 c          771 g          773 t
ORIGIN      
        1 ccggacttgt cgtaccgttg atctcgactc tgaagttctt aagatagttg ttaaccgggc
       61 tgagcagccc gtcagcggtc atcaccgcgc cgaagaagaa tccgatgatc gccgtcagag
      121 cggaaagctg tccctctccg atccggtaca cgatcccgct ggcgcatccg cccgccagca
      181 cgattccgag accaaacaga aatccgccaa gaatgttcgc aacaggcacg aaggtctggc
      241 gcataagcat cgtcgcctcg agtgcgcccg tatcggggtt gatcgcctga aggaacccca
      301 gatcttcaat gaggttcgat ccgataattg ccacgactac cgacaacaga taggccctga
      361 aaagcgtcag gtcctggatg aagatgatgt cccgaaacgc cgagttcagg cagaaccggc
      421 ctctctgcaa aacataaccg agtccgagac ccatcaggat cgcggtccca aaagaaataa
      481 cactgctatg aagcataccg tcccccctcc agctgtagtc aatgaatttt ttggcgcccg
      541 gttgtctttg cgacaatcgc gggctttttg gcagatcatt tcattacatg gttttttaac
      601 gtcggacctc tccgacgcag ttgtggcgca tgccactgtt ttcgccttct tgatccctcc
      661 cttcttcgcg gacagcgatt gatccctcag ccgtaccagt cctgccgttg aaatcgagtg
      721 cgatgaaaca attcaaagaa attgcaattt tttataaaac ctgtcttcaa aaaaaagtga
      781 aggatctcgt tacctggaaa gctgagagcc ttcactcttt gagaatacgt accctgtcat
      841 gacggggcgt ttcccgcaca tgactgatgc atcacttgca atttacagcc gcggcggctg
      901 accatgcgta tttttccgcc tcggtaggat tctttgccac gaggaccggc acgggcgcgc
      961 ccttctcaac atccttgctc acggaaccgc tgatgagcct ctgcataagc gtctttccgt
     1021 tgtagccggt gatgatgagg tcgacaccct cttcctgggc gaccttcaga atctcctcgg
     1081 acggcgcacc gtcccggacc acggtcttca cgtttaccag accgccgctg tccagctctt
     1141 tcttgtagaa actcaggatt ttttccgatc tgcggtcgag cgcttcttta tgcgccgttc
     1201 ctttcaggga ttcccggagt gtagattttt cagcttcgcc gagcatatcg atgatcatgg
     1261 acttgccctc gagctgctgc acatgcacca atacgacgct ttccggcggg cgaaccatgt
     1321 tgcggaacac cgataatacc gatgttgaac cttctgtttc gtctactgcg atcaatacct
     1381 ttttcatgag gacctcctta tgttagttgc aaacctacca ttttaatacc ttagttgaat
     1441 accttaatga atcaacaatc ccttttggag aatgacgccg agaatgaaca gcgttgccat
     1501 gattgccgtg gtgaccgaaa ctgctgccca ggcatccttc ttggtatagg cttcttcaca
     1561 catgagcgga ggcttggcca ccagtaccgg aactttcgcg tttttttcca cgtacttcgc
     1621 gacctttccg gtgatgaacc ggtcaaaccc ccttttgccg ctctgaccaa gtatgatcat
     1681 atcggccttt tcttcatcgg ccgccttcag gatttcttcg gcaggaatac cttcccgctt
     1741 tatggtcttc agcggaaaag aagcattcga agagagttcc tttgagtaaa aagcaaggat
     1801 cttatcggac cgcttgtcca gggcggcttt atagtcggtc cccttcatca tttctttcag
     1861 ggtcgacatt tccgcgtcgc caagcatatc gatcatcagg gaccggccct gcaatctctc
     1921 tacatgaagg agggtaacat cggccggctg ctggacaaaa ttatggaaaa ccgatagtac
     1981 gttctttgac cccttggtgt cgtccacagc gatcattatc ttcttattca tggtgcctcc
     2041 tgttaaacat ataaaccatc ctacaactat tgcttttata aagcaattac tatgcccgtt
     2101 ttttcaaaat acgtaaatgc ttatatttca gtatattata taatagtgat aattattatg
     2161 ttgacaaaaa catgttcaaa tgataattaa cgttaatagt gtttacttaa catttaacaa
     2221 tgtgttaatt aacgtgtttc cttttgctgc accaaaggag tcccatgatt gaaaaattct
     2281 gcaaggaccc caaattcctc atgagtgtag tcgaaaccat gagagacggc ctcatgatcg
     2341 tggacaaaga tggaactatt ctgctcttca accgtgccgc tgaggagatc acaggctatt
     2401 gcaaagaaga tgtgatcggg cagcaatgct ccattctcga cagcgacacc tgcgttgtgt
     2461 tgacagaagg aggaaagcag aggagctgtg ccctgttcaa aagcggagta atcgaaaaca
     2521 ggtaatgccg aatacggtcc cgcgacggaa gggacgtgca cctcttaaaa aatgccacga
     2581 tcctgaatga cgagaacggt gagatagtcg gagccgtgga atccatgacg gacatcacgt
     2641 cgctctacat gaaggagctt gagctcgagg agttgaaaca ggaactccgg caggagtact
     2701 ggttcatggg cctcctgggc aagagcgcgc ccatgcagaa gttgttcgaa cagatacgga
     2761 acgcggcgca aagcgaagcc ccggtcctga tctgcgggga aagcggcacg ggcaaaaacc
     2821 tcgtggccaa ggccattcat gcgctttccc gccgcaagga gggccccttt gtcggggtaa
     2881 actgcggggc cttcaacgat tacctgctcg aaagcgagct cttcggccac cggaaaggat
     2941 cgtttacagg cgccgtaagc gaccgcacag gcaggttcga ggccgcgcac aacggaagca
     3001 tcttcctgga cgagatcggc gacatgccga aggtcatgca ggtgaagatc ctgcgggtcc
     3061 ttgaagaaaa ggtggttgaa ctcgtgggag accatcggcc gatccccgtc gacatccggc
     3121 tcatctccgc gaccaacaag gatcttgaca ccctggtcaa atcagacctg
//
LOCUS       DASSLD010000588         2390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1252879, whole genome shotgun sequence.
ACCESSION   DASSLD010000588 DASSLD010000000
VERSION     DASSLD010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252879"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1190
                     /locus_tag="VFK23_10620"
     CDS             57..1190
                     /locus_tag="VFK23_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7319580.1"
                     /translation="MKRRFFGRIFFLYGLIMLAAIVLVELSITNAVRNDHLDALREHL
                     ATQATLISNDISFTPAAAFDGLCRRFKEQTGYRVTVISLGGRVLGDSDKDSSTMENHL
                     GRIEIQQALANGSGMAIRYSDTLHDDRLYVAVRISQGGRTAGFVRLSTELKAVEASIN
                     VLRMRVIVIVVAILLATGLFSIGQMERIRRLTRQIGDFSRWLASGAVGKRLYLEEAGE
                     FDEIAESLNAMSMELQNSIALNEEERKRLTVILRSIPDALLILDSQGTIQLSSSASRK
                     YFGDAPLSGKKVLEVIRSPHFLTLMEEARKNLTPGVSELRLDYPEERYFSARVSPLSY
                     KEGELSGFVVILHDITQLKKLEQTRKDFVANISHEIKTPITAI"
     gene            1203..1316
                     /locus_tag="VFK23_10625"
                     /pseudo
     CDS             1203..1316
                     /locus_tag="VFK23_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1386..1796
                     /locus_tag="VFK23_10630"
     CDS             1386..1796
                     /locus_tag="VFK23_10630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7319581.1"
                     /translation="MTLLRAKAADKNLFLKTDLPETPFKIAADRDKLIQVLTNIVDNA
                     IKFTDKGGVTVGARMENGKTILFVEDTGVGIPEKHLPRLGERFYRVDTARSRKMGGTG
                     LGLAIVKHLVKAHGWDMQIESVPEKGTKVKIHCS"
BASE COUNT          599 a          664 c          671 g          456 t
ORIGIN      
        1 aatcccgtgt acctcaagac ccgaagagga gtgggttact acgtggacga atcattatga
       61 aacggcgctt cttcggaaga attttcttcc tgtacgggct tatcatgctt gccgcgatcg
      121 tgctggtgga gctctccatc acgaacgcgg tgagaaacga ccacctcgac gcccttcgtg
      181 aacatcttgc tacccaggca acgctcattt ccaacgacat ctcgttcact cccgcggcgg
      241 ccttcgacgg tctctgcagg cggttcaagg aacagaccgg ctaccgcgtc acggtgatct
      301 ccctcggcgg gcgcgtcctt ggagattcgg acaaggactc gtccaccatg gagaaccatc
      361 ttggacggat cgagatccag caggccctcg ctaacggttc cgggatggcg atacggtaca
      421 gcgacaccct gcacgacgac cggctgtacg tcgcggtgag aatatctcag ggaggccgca
      481 ccgcgggctt cgtccggctc agcacggagc tcaaggccgt ggaagcctcg atcaacgtcc
      541 tgaggatgag ggtgatcgtc atcgtcgtcg cgatcctgct tgcaacagga ctgttctcga
      601 tcggacagat ggaacgcatc cgcaggctga cgcggcagat cggggatttt tcccgatggc
      661 ttgcaagcgg ggccgtcggc aagaggctgt atcttgagga ggcgggagag ttcgacgaga
      721 tcgcggaaag cctgaacgca atgtcgatgg aactgcagaa cagcatcgcg ctgaacgaag
      781 aggaaagaaa gcggcttacg gtgatcctgc ggagcatccc ggatgccctt ctgatcctcg
      841 actcccaggg gacaatccag ctctcaagct ccgcctcgag gaaatacttc ggggacgccc
      901 ccctgagcgg aaagaaagtc ctggaagtca tcagaagccc tcatttcctg acgctcatgg
      961 aagaggcccg caaaaacctc acgcccggcg tcagcgaact gcggctcgac tatcccgaag
     1021 aaaggtattt ttcagcacgg gtctcaccgc tctcctacaa ggagggggaa ctttcaggct
     1081 tcgttgtcat cctgcacgac atcacgcagc tcaagaaact ggaacagacg cgaaaggatt
     1141 tcgtcgcgaa catctcgcac gagatcaaga cgccgataac ggcaatctag ggcttcgccg
     1201 acaccctgct ggagggcgcc ctccatgacg cggaacatgc cgaaaagttc ctgcagatca
     1261 tcaagtcgaa cagcgagcgg atcaacagcc tggtggacga cctcatgacg atctcgaaaa
     1321 tcgagctcgg cgcggcaagg atcgagaagg cgcccgtgga ggtcacggac gcgatcgaac
     1381 aggtgatgac cctgctccgg gcaaaggccg cggacaagaa ccttttcctc aagaccgatc
     1441 ttccggagac gccttttaaa atagccgcgg acagggacaa actcatccag gtgctcacga
     1501 acatcgtgga caatgcgatc aaattcacgg acaagggcgg ggtgacggtc ggcgcaagaa
     1561 tggaaaacgg caagaccatc ctcttcgtgg aagacacggg ggtcgggatt ccggagaagc
     1621 atctgccccg cctcggcgaa cgtttttacc gcgtggacac ggcccggtcg aggaagatgg
     1681 gcggcaccgg gctcggcctg gcgatcgtca aacatcttgt gaaggcccat ggctgggaca
     1741 tgcagatcga aagcgttcct gaaaagggga cgaaggtgaa gatccactgc tcgtaaaagg
     1801 cagataacgc gttccatacc gtcgtccgca gtatatccct atccgcgaaa taaatttctt
     1861 ttcatcaact tttcatcaag tcgtcatact gctgcaacat cagtccgcta tattccatcc
     1921 cgctacaaaa agccgcgaag catcaattcc gtaaaaggga ccactcatag gcctgcgcgg
     1981 atttctctct caataacaac ctccgggttt tttggaaacg gctctccacg gttggacggg
     2041 ctgggcgagc ggagatgtcg tataccgaca ataactccgc agaagtgctt cgcagaggag
     2101 gactcatcaa agaaggaggt gaatcccgag gcatttttta atggaaactt aacaagcttg
     2161 taacaatcct gtaacacacc tctggtataa aatagcggtt ataataatga aaaggagata
     2221 acatatgaag cggactctgg caattatcgt actcttaatc ggaatgctga cgatcaaccg
     2281 ggcgcaggcg gaagagatgc tgaacggcgc aggggccacg tttccctatc ccgtgtattc
     2341 cgcatgggcc catgaataca acaaggtaac gggcatgaag ctcaattacc
//
LOCUS       DASSLD010000589         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1088904, whole genome shotgun sequence.
ACCESSION   DASSLD010000589 DASSLD010000000
VERSION     DASSLD010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088904"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VFK23_10635"
     CDS             <1..488
                     /locus_tag="VFK23_10635"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HET7319582.1"
                     /translation="RSCWVDAWAVGRIIDTAQSERKRDAQPSSRALDLAEKAILRYTG
                     PFLADEMNKAWSVSPREKLKSNCIKMVNLLVSHWKSRGSWDKAIEYLSKGLEIDELAE
                     DLRRELMMCYRAIGREIDAIKTYRHFREVLAKALGIDPSPSTEALYRKLIEEKRKPSS
                     H"
     gene            773..2116
                     /locus_tag="VFK23_10640"
     CDS             773..2116
                     /locus_tag="VFK23_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319583.1"
                     /translation="MRAARADNCDLEDGANIAVIGGGPAGSFFSIFALKMAKMVGKDL
                     NITIFEPKDFTKTGPAGCNHCGGVISELLVQTLAVEGINLPDTVVRKGINSYRLHTNY
                     GSVFIATPSFDKTIATVYRGGGPKGITVADKESFDHFLLNRAIQEGAVHKPFLIDHIE
                     WRNGRPVLFSRKQELMVADLAVGAFGLNSATAKLVEDMKFGYSGPRVLTAAIAEIAMG
                     GDVVAEHFGSSIQLFLLPNPEITFAAMIPKGAYVTLCILGNPAGAMHVKTVDDFLELA
                     AVKRVLPKNASYHVACRCLPKLNVSAPKKAFTDRVVMCGDAGATRLFKDGLGAAYLMG
                     KAAAKTAVFQGIGERQFAEGYYPAYKRTIRDNYYGNILFTVIGIYRKFGLLTKAMLKV
                     VEKEQKGSRKEKNMSSILWDMFTGNEKYRKVFSHAFNVKMQVDVLEGLLKSGLGR"
     gene            2119..2985
                     /locus_tag="VFK23_10645"
     CDS             2119..2985
                     /locus_tag="VFK23_10645"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7319584.1"
                     /translation="MREGELGRVYTNGEAICREGENGEAMYVIQSGKVKITKHSHTGE
                     LTLATLESGDIFGEMTLFDKLPRSATATALGEARVLSVDKKKLFATISNDPTLVFKIL
                     ETMSHRIRRLTETLADISETKTDMLKIFCNVEDTCQMILTEAKDIVKAENGSIMMYDE
                     KTNCLAIRAAFGVESDKKLTFAAGEGIAGDVLKSGRAELVNNVDIDRRFKPGDIRLRS
                     ILCAPIKCGADHVGVMNMSLCSDRLFTVDDLKEFSSLTTSAGMAIQNAKNFSSLRNAA
                     DRILKHATMLNM"
BASE COUNT          854 a          877 c          899 g          670 t
ORIGIN      
        1 cgcgatcgtg ctgggtggat gcctgggccg ttggccgaat cattgacacc gctcagtcgg
       61 aacggaaaag ggacgcacag ccctcaagcc gggctcttga tctcgcggaa aaggccatcc
      121 tgcgctatac cggccctttc cttgccgacg aaatgaacaa ggcgtggtcc gtgtccccgc
      181 gggaaaagct caagagcaac tgcatcaaaa tggtaaacct cctcgtctcc cactggaaga
      241 gcaggggctc ctgggacaag gccatcgaat acctgtcgaa gggccttgaa atcgacgagc
      301 tggccgaaga cctccggcgc gagctcatga tgtgctaccg ggcaatcggt cgggaaatag
      361 atgcaattaa gacataccgc catttccggg aggtactcgc taaagcactg ggtatagatc
      421 cctccccgtc caccgaagcc ctttaccgga aattgatcga agaaaaaagg aaaccgtcat
      481 cccactgacc atcaaaaaac catccgcgct ctccgaaggg ttttccgtcg gattcgtttc
      541 cctggtcacc gaggcaatat taaagtacga atcctgcaac acttgtccga ttaatgcaca
      601 cgaatgaagc ttgcaaagcc ctaaacttga caacgaaccc cgcccgtgat tgaataataa
      661 tattttccgg aatccttttt cacgataaac tcattggacg tttttttgca aatcgacata
      721 cgtgccaatg gggcgctcag gaaccctctt ttttccgaca aaggcggtct ccatgagggc
      781 agcgagggct gataactgcg atcttgaaga cggcgcgaac atcgcggtca taggcggcgg
      841 tcccgcaggc tcattcttca gcatctttgc tctcaaaatg gcgaagatgg tgggtaagga
      901 tttgaacatc accatcttcg agcccaagga tttcaccaag acaggcccgg ccggatgcaa
      961 ccattgcggc ggggtcatct ccgagctgct cgtacagaca ctggctgtcg agggcatcaa
     1021 cctccccgat accgtggtcc gcaaaggcat taattcctac agactccaca ccaactacgg
     1081 aagcgtcttc atagcgaccc catccttcga caaaaccatt gctaccgtct atcgcggcgg
     1141 aggaccgaaa ggcatcaccg tcgccgacaa ggaaagcttc gatcattttc tcctgaaccg
     1201 cgcgatccag gagggggccg tccacaagcc tttcctgatc gatcatattg aatggcggaa
     1261 cggaagaccc gtgctgtttt cgcgaaagca ggaactcatg gtggcagatc ttgccgtagg
     1321 cgccttcggt ttgaacagcg cgacggccaa gctggtcgag gacatgaaat tcggatacag
     1381 cgggccacgc gtcctcaccg cagccatagc ggagattgcc atgggcggcg atgtcgttgc
     1441 cgagcatttc ggcagttcca ttcaactctt cctcctcccc aatcctgaga tcacgtttgc
     1501 tgctatgata ccaaaggggg cctatgtgac cctctgcatc ctcgggaatc cagcgggtgc
     1561 gatgcatgtg aaaaccgtgg atgattttct cgaacttgct gcggtgaaga gggtgctgcc
     1621 caaaaatgca tcttaccacg tggcttgccg ttgcctgccg aagctgaacg tcagtgcgcc
     1681 gaaaaaggct tttaccgacc gggtggtgat gtgcggcgat gccggggcca cgaggctgtt
     1741 caaggatggt ctgggcgccg cctaccttat gggcaaggcc gcggcaaaga ccgccgtatt
     1801 ccagggcatt ggagagcggc agtttgccga aggctattac ccggcctata aaagaaccat
     1861 ccgcgataat tattacggaa acatcctctt taccgtgatc ggtatctacc ggaagttcgg
     1921 attactgacg aaagcgatgc tgaaagtcgt ggaaaaagag cagaaaggca gccgcaaaga
     1981 aaaaaacatg agcagtatcc tctgggacat gttcaccggc aacgagaaat acaggaaggt
     2041 attttcgcat gcgttcaacg tcaagatgca agttgatgtg ctggaagggc tgctgaaaag
     2101 cgggctcggg aggtgactat gagagaggga gagctcggac gggtatacac caacggcgag
     2161 gcgatctgca gagaaggaga gaacggcgag gcgatgtatg tcatccagtc ggggaaggtg
     2221 aagatcacca agcactccca cacaggagaa ctcacgcttg ccaccctcga gagcggcgat
     2281 atcttcggag agatgacgct tttcgataag ctgccccgct ccgcgacggc cacggccctg
     2341 ggagaagcga gggtcctgag cgtcgacaaa aagaagctct tcgcgacgat cagcaatgac
     2401 ccgacactgg tattcaagat actcgagacc atgagccatc ggatcaggag gctgaccgaa
     2461 accctggcgg atatctcgga aacaaagacc gacatgctga agatcttctg caatgtggaa
     2521 gacacctgcc agatgatcct gaccgaggca aaggacatcg tcaaggccga gaatggttcc
     2581 atcatgatgt atgatgaaaa gaccaattgc ctggcaatta gagccgcctt tggcgtcgag
     2641 tccgataaga agctcacctt cgcggcgggt gaaggcattg ccggcgatgt gctgaagagc
     2701 ggcagagcgg agctggtgaa taacgtcgac atagaccgcc gcttcaagcc cggtgatatc
     2761 cggctccggt ccatcctgtg cgctcccatc aaatgcggcg cagatcacgt cggcgtcatg
     2821 aacatgagcc tctgttcgga ccggctcttc accgtcgacg atctcaaaga gttcagttcc
     2881 cttacgacct ctgccggcat ggcgatccag aacgcaaaaa acttttcaag tctccgaaac
     2941 gctgcggaca ggatactgaa acacgccaca atgttgaata tgtaaaccgg atgctcatgg
     3001 tgccgaataa tgatccattg tatcaatggc agcattctct gaagtcgttt gcatatgaac
     3061 aattgggttt cagaaccacg agagctgcgt aaccaaaggg cgaaaaaaga aaactcctgc
     3121 tcttcattaa gactgggaga gattccatgg cggataacaa aagcacggaa ttagggagga
     3181 cgaggagtgg ttttcagccg aatgtcgcag cggcgctttc atatgctttg acagtagcaa
     3241 ccggtacact tatttttgtc attgaaaaag aaaacaaata tgcccgattc catgcgttgc
//
LOCUS       DASSLD010000590         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_485494,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000590 DASSLD010000000
VERSION     DASSLD010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485494"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VFK23_10650"
     CDS             complement(<1..985)
                     /locus_tag="VFK23_10650"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET7319585.1"
                     /translation="MSDPRTIIEALEPCRTILISVHRNPDGDALGSQLALMLALEKMR
                     KTVTAHNLDPVPEVYRFLPQADRIRAGRPVPSHYDAHIVLDGEPSRTGLFDGTYPAHT
                     LINIDHHVTNPRSWQLTWLDEDASATGEMIYKLIRHLDVPIDRDIALCLFTSIFTDTG
                     SFRYTNTTPECMRISAELLEAGADPWLVTENVYESFAFRRIKLLGAVLSSLERSADGR
                     VAWVVVTDELYRRTGTTSEDTENFINFVRSIKGVEVAVLLRQIGEAQYKISLRSKGRV
                     DLSALAQSFGGGGHKNAAGGVMSGTIEVVTSKVINEVDKAVRAQLGSGSRIP"
     gene            complement(978..1352)
                     /gene="rbfA"
                     /locus_tag="VFK23_10655"
     CDS             complement(978..1352)
                     /gene="rbfA"
                     /locus_tag="VFK23_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET7319586.1"
                     /translation="MSKPTFKRSERVSDQMKHEIADILMRKIKDPRIGFVTVTDVEVA
                     DDLRNAKVFVSVYGGDKEQTLKGLKSATAFIRLELGKRMRMRFIPELLFRFDSTLERG
                     AHIMELLRELEEDKEKKDPTDE"
     gene            complement(1448..1729)
                     /locus_tag="VFK23_10660"
     CDS             complement(1448..1729)
                     /locus_tag="VFK23_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HET7319587.1"
                     /translation="MFIGVCTIELHVPESGSLKNKRHSIRSLKDRVRNKFNVSVAEIE
                     DNDLWQKATLAVAAVSNDKAHLNQTLDHVLNMVRGVPEVDLLDYQIEIF"
     gene            complement(1865..>2243)
                     /locus_tag="VFK23_10665"
     CDS             complement(1865..>2243)
                     /locus_tag="VFK23_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HET7319588.1"
                     /translation="RLYNIIYNAIDDIKAAMEGLLEPTLKERILGRAEVRQTFHVSKV
                     GTIAGAYVTDGVMTRQNDGVRVLRDNVQVYEGKLHSLKRFKDDVREVQSGYECGIGVE
                     NFNDIKVGDVIEAYIIDKVAAKL"
BASE COUNT          454 a          626 c          616 g          547 t
ORIGIN      
        1 ccgggatgcg gctcccgctg ccgagctgcg cccggactgc cttatcgacc tcattgatca
       61 ccttgctcgt caccacttcg atcgtcccgg acatcacgcc tcctgccgca ttcttgtgtc
      121 cgccgccgcc gaacgactgg gccagcgccg agagatcaac gcgccctttc gaacgcaggc
      181 tgatcttata ctgcgcctcc ccgatctgcc tcaggagaac cgcgacttct acgcccttaa
      241 ttgaccgcac gaaattaatg aaattttcgg tgtcttccga cgtcgtgccg gtccggcggt
      301 agagttcgtc ggtcacaacg acccaggcga cccggccgtc ggcgctgcgc tccagggagg
      361 aaaggaccgc cccgaggagc ttgatgcggc ggaatgcgaa ggactcgtac acattttccg
      421 ttacaagcca agggtcggct cctgcctcga ggagctctgc cgagatccgc atgcattccg
      481 gcgtggtgtt cgtataccgg aaggagccgg tatcggtgaa aatggacgta aaaaggcaga
      541 gcgctatgtc gcggtcgatc gggacgtcga gatggcggat gagcttgtaa atcatttccc
      601 cggtcgcgga cgcgtcttca tcgagccagg tgagttgcca tgaccggggg ttggttacat
      661 ggtggtcgat gttgatgagt gtatgcgcgg gatatgttcc atcgaaaaga ccggttcgcg
      721 aaggttcgcc gtcgagcacg atatgggcgt cataatgact cgggacgggg cggcccgctc
      781 ggatacggtc tgcctggggc agaaaacggt agacctccgg caccggatcg aggttgtgcg
      841 cagtgacggt cttccgcatt ttttcgagcg cgagcatgag cgcaagctgg gaaccgagcg
      901 cgtccccgtc gggatttctg tggacggaaa tcaatatcgt cctgcagggc tccagcgcct
      961 cgatgatggt tcggggatca ctcatcggtc gggtccttct tctccttgtc ctcttcaagt
     1021 tcgcggagca gttccatgat gtgagcgccg cgttccagcg tggagtcaaa acggaagaga
     1081 agctcaggga taaacctcat cctcattctt tttccgagct ccaagcgaat aaaggccgtc
     1141 gcgctcttga gacccttgag ggtttgctcc ttgtccccgc cgtagacgct aacgaacacc
     1201 tttgcgtttc tcaggtcgtc tgcaacctcg acatcggtca ctgtaacgaa accgatacgg
     1261 gggtccttga tcttgcgcat gaggatgtct gcgatctcat gtttcatctg gtcgctgacg
     1321 cgctctgatc ttttaaaggt cggtttggac atgggttaaa atcaggagcc ggaagtcagg
     1381 agccagtagc cagaaggaat gtgaccttct cctgcactct gactcctgac ttctgtctcc
     1441 tgccttttta gaatatctct atttgataat ccagaagatc gacttcggga acgccgcgta
     1501 ccatgttcag aacatggtca agcgtctggt tcaggtgggc cttatcgttg ctcactgcgg
     1561 ccacagcaag cgtcgccttc tgccacaggt cgttatcttc gatttccgca acggagacgt
     1621 tgaacttgtt ccgcaccctg tctttcaggc tgcggatcga atgcctcttg ttcttgagcg
     1681 aaccgctttc cggcacatga agttcgatag tgcatactcc gataaacata tcaattatgt
     1741 aaaaacccta aactccaata tcgaaactcc aaacaaattg aacgtcagaa agaaatcttc
     1801 aaggccgttt gaatttccga ttttgatttt gtccagaatt gagaaattcg gatttagggt
     1861 ttgtctatag ctttgccgcc accttgtcga taatataggc ctcgatgacg tcgccgacct
     1921 tgatatcgtt gaagttttca acgccgatgc cgcattcata acccgattga acttcccgca
     1981 cgtcgtcctt gaaccgtttg agcgaatgaa gtttcccctc gtagacctgg acgttgtccc
     2041 tgagcaccct cacgccgtcg ttttgtctcg tcatgacgcc gtcggtgaca taggcgccgg
     2101 caatagtgcc gaccttcgag acatggaacg tctggcggac ttccgcccgg cccaggatgc
     2161 gttccttgag cgtcggttcg agcagtcctt ccatggccgc cttgatgtca tcgatggcgt
     2221 tgtagatgat gttgtacagc cgg
//
LOCUS       DASSLD010000591         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1430764, whole genome shotgun sequence.
ACCESSION   DASSLD010000591 DASSLD010000000
VERSION     DASSLD010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430764"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VFK23_10670"
     CDS             <1..513
                     /locus_tag="VFK23_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7319589.1"
                     /translation="RQGTFMPNQFDNPANVRAHYETTGRVVYEQTGGEVNVFVAGMGT
                     TGTLMGAGRRLKEFDSSITVLGVEPNLGHKIQGLKNMTEAIVPKIFDPKALDEKWNVQ
                     DDDAFNTTRRLALEEGLFVGMSSGAAMWAAVKKAKEMKDGTIVVILPDRGDRYLSTAL
                     FTSICGKCPP"
     gene            754..1203
                     /locus_tag="VFK23_10675"
     CDS             754..1203
                     /locus_tag="VFK23_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7319590.1"
                     /translation="MGLEIRVRDVMTTNVLAISKYDSIVKVANILSEKNISGLPVVDK
                     ENKVIGIITQADILSMVGVSREHTFKDLLKYMLGEPLHERKIGDHVGDIMTSPALTVK
                     PDANIAEAVRIMTEKRIRRLTVVDGNNTLIGIITRADILKAVIQKLK"
     gene            1345..1722
                     /locus_tag="VFK23_10680"
     CDS             1345..1722
                     /locus_tag="VFK23_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020394928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModE family transcriptional regulator"
                     /protein_id="HET7319591.1"
                     /translation="MPSKKTGHRHAKTKPNPGFRGRVWIDGPEGTFIGYGRVVLLERI
                     REHGSITKAAKSMEMAYRHAWDLVDSMNRQAEEPFVELATGGKGGGGARVTEAGERAV
                     KLFRRFHDDLQSFLKREEKKLRL"
     gene            1748..>2370
                     /locus_tag="VFK23_10685"
     CDS             1748..>2370
                     /locus_tag="VFK23_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7319592.1"
                     /translation="MRRIIVFALALTLAGTAAFAETRIRMASTTSTQNSGLFEHLLPL
                     FEKKTGIKIDVVAVGTGAAIEIGKRGDADVVFVHAKEQELKAVEEGFFVNRHDVMYND
                     FVVIGPSNDPAKIKGMKSAPDAFKKIAESGTPFVSRGDKSGTNTKELSLWKKTGIEPA
                     GQKWYLDVGQGMEKTQRIADEKRAYTLTDRGTWLATKDKDKLDMTVVL"
BASE COUNT          570 a          649 c          698 g          453 t
ORIGIN      
        1 cggcagggca ccttcatgcc gaaccagttc gacaaccccg cgaacgtccg ggcccactac
       61 gaaaccacgg gcagggtggt ctacgagcag accgggggcg aggtgaacgt gttcgtcgcg
      121 ggcatgggca cgaccggcac gctcatgggg gcgggcagga gactcaagga attcgacagt
      181 tcgatcaccg tcctgggcgt ggagccaaat ctcggccaca agatccaagg cctcaagaac
      241 atgacggagg caatcgtgcc gaagatcttc gacccgaagg cactggacga aaaatggaac
      301 gtccaggacg atgacgcttt caacacgacg cgcaggctgg cgctcgaaga aggactcttc
      361 gtcggcatga gcagcggcgc ggccatgtgg gctgcggtca aaaaggcgaa ggagatgaaa
      421 gacggcacga tcgttgtgat cctacccgac cgcggcgatc ggtacttgag cacggcgctg
      481 ttcacctcaa tctgcggcaa atgtccgccg tagttactgc agaattgaat tcctcgtaaa
      541 atggaaagaa atattgccac agagtacaca gagcgcacgg agaaaagccc gatatctttt
      601 ttttatttca ggtctgaatc tctctgtgtt ctctgtgctc tctgcggcta aaaagctttt
      661 ccgacttgtc cggttttcaa tgatattttt tatatcggta tggagggcat tataatgagt
      721 gcggatcaaa gcggcggaca tatacgttca tccatgggac tcgagattcg cgttcgcgac
      781 gtgatgacga cgaacgttct tgccatctcg aaatatgaca gcatcgtgaa agtggcgaac
      841 atcctgtcgg aaaagaacat cagcggtctg ccggtcgtgg acaaggaaaa caaggtgatc
      901 ggcatcatca cccaggcgga catcctctcc atggtgggcg tgagccggga gcataccttc
      961 aaggacctct tgaaatacat gctcggagag cccctccacg agcgcaagat cggcgatcac
     1021 gtcggcgaca tcatgacgtc gccggccctt accgtaaaac ccgacgcgaa catcgccgag
     1081 gccgtgcgga tcatgaccga aaagcggatc agacgcctga ccgtggtaga cggcaacaac
     1141 acgcttatcg gcatcataac gagggcagat atcctgaagg cggttatcca gaagctgaaa
     1201 tgaaaccacg ccgggacctc ccggccggcc tgcacggctt tcagcaaagc cgcggaacat
     1261 cgattccgcg gctttttgtt tgtgcgcagc gggcgacccc gcttttttct tgccacccgt
     1321 tattccctct gctatattac ggtcatgcct tccaaaaaga caggccatcg acacgcaaag
     1381 accaagccga acccggggtt ccggggccgc gtctggattg acgggccgga agggaccttc
     1441 atcggctatg gccgggtcgt tcttctggaa cggatcaggg agcacggctc cattacaaag
     1501 gccgcgaaat ccatggaaat ggcataccgc catgcctggg acctggtgga ctccatgaac
     1561 agacaggcag aggagccctt tgtcgaactg gccacgggcg gcaagggggg cggaggcgcc
     1621 cgggtgaccg aggccggcga gcgggcggtc aagctgttcc ggcgttttca tgacgatctt
     1681 cagtcatttt tgaaacggga agagaaaaaa ctcaggcttt gaaaatttca ttcgggaggc
     1741 ttgttctatg agaagaatca tcgtttttgc ccttgcactg actcttgctg gcacggcggc
     1801 ttttgccgag acacgcatcc gcatggcgtc caccaccagc acccagaact cagggctatt
     1861 cgagcacctg cttcccctct tcgaaaagaa gaccggcatc aagatcgacg tggtggccgt
     1921 gggcaccggc gcggcaatcg agatcggaaa gcgcggtgac gctgatgttg tcttcgtcca
     1981 cgccaaagaa caggagctga aggccgtgga ggaaggcttt ttcgtgaacc gccatgacgt
     2041 gatgtacaac gactttgtgg tcatcgggcc gtcgaatgac ccggcaaaga tcaaaggcat
     2101 gaagtctgcg cccgatgctt tcaagaagat tgcggagtcg ggaacgccct tcgtgtcccg
     2161 cggggacaag tccggcacga acacgaaaga actctccctc tggaagaaga ccggcattga
     2221 accagccggc cagaaatggt atctcgatgt cggccagggc atggagaaga cccagcgcat
     2281 cgccgacgag aagcgcgctt atactcttac ggaccgggga acctggctgg ccaccaagga
     2341 caaggataag cttgatatga ccgtcgttct
//
LOCUS       DASSLD010000592         4782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1483741, whole genome shotgun sequence.
ACCESSION   DASSLD010000592 DASSLD010000000
VERSION     DASSLD010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483741"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..514)
                     /locus_tag="VFK23_10690"
     CDS             complement(65..514)
                     /locus_tag="VFK23_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319593.1"
                     /translation="MKVLGIHLAKGQLRYALLDGTKEAPILVEKGRLLTTDPADVPQL
                     MAWYDTQFRQLLDRLTPDKIAYRVALSPRKEQLFTSEFPFGILNLFAKQRNLPIIPYT
                     PLSFVASKLGLPKGTDLSIACDVAFGTHPPYWDAHQKLSILVAWFEL"
     gene            complement(659..1759)
                     /locus_tag="VFK23_10695"
     CDS             complement(659..1759)
                     /locus_tag="VFK23_10695"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HET7319594.1"
                     /translation="MVSTAAFVLSGLIIGAAFGFVLQRGRFCMNSAYREVLFGDYTML
                     RAYLLAVVVTMIGANLIEDMGWLRHMNEAGAVVTGGLERQAFVPWANIIGGYVFGMGI
                     VLAGGCGSGIVYRIGEGNLAYVVAVFSFFFGISATKFGTLKPVYDLLVSIRWPKEGPA
                     PTLYSLLGVNKWVVIAALSVVFLVFVFQGRPSLKAKKGYSWSVAGILVGLIAVAAWWA
                     SEYFGGRARGLSFTGPVREFFLTVIFGNYLNDPSAVVPAESRSVLGVTFSWSAVYVAA
                     VPVGAYISARLLHEFGLKVPPVGDLLRGIYGGFLMGVGAEIGGGCNIGHGLTGVSTLA
                     LSSWVANAFIFLGDWTMVYFLFIRPLNRKAGS"
     gene            complement(2083..2706)
                     /locus_tag="VFK23_10700"
     CDS             complement(2083..2706)
                     /locus_tag="VFK23_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7319595.1"
                     /translation="MSESCYDRHILPYLIELTCGNKAVSEQREKVVPLARGRVLEVGI
                     GTGLNMPHYDKSKVEKIIGLDPALHMHRLARRRIARAGLPVELIGLSAEKIPLDDASI
                     DTVLVTYTLCTIPDPVAALKEMHRVLAPGGKLIFCEHGRAPDESVRRWQDRLTPYWKR
                     FTGGCHLNRDIPAILAEAGFQCRDMQSMYFPGPRPLTFNYWGDAYPA"
     gene            complement(2781..3248)
                     /locus_tag="VFK23_10705"
     CDS             complement(2781..3248)
                     /locus_tag="VFK23_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7319596.1"
                     /translation="MNSSITIEQALRAKGHGYFAVPPNTTAYEALEFMAEKNIGALLV
                     VEDGRLTGVFSERDYARKVILKGKSSKSTTVGELMSRPPICGCPELTIRDCMALMTDH
                     HIRHLPIMEQGTIVGLVSLGDVVKAVVSELDSTVQTLESYVTGNDYRARLYCP"
     regulatory      complement(3312..3389)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3732..4595)
                     /locus_tag="VFK23_10710"
     CDS             complement(3732..4595)
                     /locus_tag="VFK23_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319597.1"
                     /translation="MKIKGLIVLVLGLAVTTLAACSSGGGSDSSSPEVQLKGIASKGP
                     LNNADVKVFAIKNGVVDTSAAIGTGTTDAKGNFNVTLSSAFSGAGAVEVLVTGGTFTD
                     EATGVPGVGLKTELRAMVTNVSAGATKVVAVTPLTELASKKAETTAAGLSSGLTTTAI
                     DDSNKSISDTFQIGDDITSTLPKSDGTVTEKKHATACAAISQIVNNRKQSGESTDDAL
                     TRVMGELETEIETHGGLSTSTETEIHTAETEVETQSGGGGGGGGGGGGGGGGGDVTSQ
                     TTTTTTTTTGK"
BASE COUNT         1029 a         1427 c         1278 g         1048 t
ORIGIN      
        1 caacaacgtc tctctgtgtc ttcttgaaaa atggtccctg cctcagtaaa aatgcggggt
       61 gcgatcagag ttcgaaccat gcgaccaata tcgagagttt ctgatgcgca tcccaatagg
      121 gcggatgagt cccgaaagca acgtcacatg caatactgag atcggtcccc tttggcaggc
      181 cgagtttgga cgctacaaaa gatagcggtg tatagggaat gatcggcagg ttacgctgtt
      241 tcgcaaagag attaaggatg ccaaagggga actcggaggt aaacaactgc tctttccgtg
      301 gcgacagtgc gacgcggtat gcaattttat ccggcgtcaa acggtccagc agctgccgga
      361 actgggtgtc gtaccaggcc atcaattgcg gcacatcggc agggtccgtg gtgagcagac
      421 ggcctttttc gacaaggatg ggcgcttctt tcgttccgtc gagaagagcg tatcgaagct
      481 ggccctttgc caggtgaatg ccgagaactt tcataagggg aactcctgtg caattccaac
      541 acagacaaag tgtcaggtct ttacatttag cagcagccac tattacggct tggaaaagag
      601 ggcaatgtca acagttaaga cctgccccta agagttcgtt gtcgcgaaat gagcggccct
      661 acgatccggc cttacggttc agcggcctga taaaaaggaa atacaccatc gtccagtcgc
      721 ccagaaagat gaaggcattg gccacccagc tcgagagcgc gagggtagaa acaccggtga
      781 ggccgtggcc gatattgcac ccccctccga tttccgcgcc gacccccatc aggaacccgc
      841 cgtagatccc cctcagcagg tctccgaccg gcgggacctt gagcccgaac tcatgcagaa
      901 gcctggcgct gatgtacgcg cccaccggga cggccgcgac ataaaccgcg ctccatgaga
      961 acgtcacgcc gagcacggac ctcgactcag ctgggaccac ggctgaggga tcattcaggt
     1021 agttgccgaa aatcacggtg agaaagaact ccctgaccgg cccggtgaac gagagaccgc
     1081 gcgcccggcc gccgaaatac tcggacgccc accacgcggc cacggcaatg aggcccacca
     1141 ggatgccggc cacggaccag gaatatccct tcttcgcttt cagggacggc ctgccctgga
     1201 acacgaagac caggaacaca acggagagcg ccgcgatcac gacccacttg ttcaccccga
     1261 gcaggctgta aagcgtcggc gccggtcctt ctttcggcca ccgtattgac accagcaggt
     1321 cgtacaccgg cttcagggtg ccgaatttcg tcgcggaaat accgaagaaa aagctgaaca
     1381 cggcgacgac gtatgccagg ttcccttcgc cgatgcgata gacgatgccg cttccgcatc
     1441 cgcccgcgag cacgatgccc atgccgaaaa catacccgcc gatgatattg gcccacggaa
     1501 cgaaggcctg ccgttcgagc cccccggtca ctacggcgcc ggcctcattc atgtgcctga
     1561 gccagcccat gtcctcgatg aggttcgcgc cgatcatggt gaccaccacg gcgagaagat
     1621 aggcgcggag catcgtataa tctccgaaca ggacctcccg gtatgcggag ttcatgcaaa
     1681 aacggccgcg ctgaagcacg aaaccgaacg ccgcgccgat aatcaggccg gataggacaa
     1741 acgctgcggt cgataccatg tcccgcctcc ttgaaggaac caccctgcgg agctcttcac
     1801 atggttttcg gacctttgtg tgtatgggca gatgccggac ggtatcgacg cttcacgcgc
     1861 aaattgcgag ggaaaccatc ctcttaaaat tatatcactc aggaaaatgt cgacgcgcga
     1921 ttccgaatgt ctccccggtt ccacgcgtcg taaggcggga tgaaggcact tctttatcct
     1981 tgtaagggtt ttgcagcgta agtaacagcg tcctcgaagt tattcgactc gaccgcgtca
     2041 tactggctgc cgcttagaga acctgctgtc cctgcaattg gctcaggccg ggtaggcatc
     2101 accccaatag ttaaaggtga gcggccgcgg gccgggaaaa tacatgctct gcatgtccct
     2161 gcactggaat ccggcttcgg ccaggatggc aggaatgtcg cggttgaggt ggcagccgcc
     2221 ggtaaatctc ttccaatacg gcgtcagcct gtcctgccag cggcggacgc tctcgtccgg
     2281 tgcgcggcca tgttcgcaga aaatcagttt cccgcccggc gcgaggacgc ggtgcatttc
     2341 cttcaacgct gcgacgggat cggggatggt gcacagcgta taggtaacca ggaccgtatc
     2401 gatgctcgcg tcatcgagag ggatcttttc ggcagagagg ccgatgagct ccacggggag
     2461 tcccgcgcgg gcgatacgcc tgcgtgcaag tctgtgcatg tgcagcgccg ggtccaggcc
     2521 gatgatcttc tccacttttg acttgtcgta gtgcggcatg ttcagcccgg tgccgatgcc
     2581 gacctccagc acgcggcccc tggccagcgg cacgactttt tcccgttgtt cggaaacggc
     2641 tttattgccg caggtaagct cgatcaaata ggggagtatg tgccggtcgt agcagctttc
     2701 gctcatgtgc ccttcttccc cgctttcgca gccgggactc agcggccgca tcatgacgct
     2761 ccatggatcc cggggtttgc tcagggacaa tacaaccgcg cccggtaatc attgccggtg
     2821 acgtagctct cgagtgtctg gaccgtcgag tccagctcgg aaaccaccgc tttgaccacg
     2881 tcgccgagac tcacgagacc cacgatcgtg ccctgctcca tgatgggcag atggcggata
     2941 tggtgatcag tcatgagcgc catgcagtcc ctgatcgtaa gctccggaca cccgcagatc
     3001 ggcggcctgc tcatcagctc cccgacggtc gtgctcttgg aggatttgcc tttcaagatc
     3061 accttccgcg cataatcccg ctccgagaaa acgccggtca gccttccgtc ctcgacgacc
     3121 aggagcgccc cgatgttctt ctccgccatg aactccaggg cctcatatgc ggtcgtgttc
     3181 ggcgggacgg cgaagtagcc gtgtcccttc gcgcgcagcg cctgttcgat agtaatcgac
     3241 gaattcattt ctcctacctc cttctttcac gggcctgaat aaaaaaagcc gggcataccg
     3301 aaggttattc cttcggcagc tcggccatct tctctttaag acccgccgct ttcctcgccc
     3361 ttctcgcgaa aggatcggct ttgtcgggaa atatttttcc tgaatgttat gatactcaat
     3421 accactgcat ctcttgcctg tcaaagtcta tttctaaatg cagacagtct acagtcactt
     3481 gttggtacga aataagtaac agcggagttc ggaacgaata ataaaaaacc cgcctgagag
     3541 attatcctcc gacgggtttt catactgcat cgttgtttaa ttccagagcc aacggccgct
     3601 catgatacaa atgcggttgc accggtaatc ggaactttgc tgcttgtttt cacatccctt
     3661 cggggcgccg caatgttctc atagtctcgg ccgggtagta agcccggccg agatccccgg
     3721 cttgtgctga tttattttcc cgttgtcgtg gttgtcgtgg tagtagtctg gctggttaca
     3781 tcaccaccac cgccaccacc gccacctccg ccgcctccgc cgccaccacc gccgctttgc
     3841 gtctcgacct cagtttccgc agtgtggatt tcggtctcag tacttgtcga taggccgccg
     3901 tgagtttcga tctcggtttc caactcaccc atgaccctcg tcagcgcgtc gtccgtcgat
     3961 tcgccggact gctttctgtt gttcacaatc tgagaaatag cggcgcatgc cgtcgcgtgc
     4021 tttttctccg taacagtgcc gtccgacttg ggcagcgttg aggttatatc gtcgccaatc
     4081 tggaaggtgt cggaaatcga cttgtttgaa tcatctatcg ccgtggtcgt caacccggaa
     4141 ctcaacccgg cagctgttgt ctctgctttc ttacttgcaa gctcggtcag cggcgtcact
     4201 gctactactt tcgtagcacc cgccgatacg ttcgttacca tagccctaag ttcggtctta
     4261 agcccaacgc cgggaacacc ggtcgcttca tcggtgaacg tccctccggt caccagcact
     4321 tccaccgcgc cggcaccgct gaatgcagac gacaatgtaa cattgaaatt ccctttagca
     4381 tcagtcgttc ccgtaccgat cgccgcggac gtgtcaacga ccccgttttt tatggcaaat
     4441 actttcacgt cagcgttatt gagcggcccc tttgatgcta tacctttgag ctgaacctct
     4501 ggtgaagagc tgtcactgcc gccccccgaa ctgcaagctg cgagcgttgt cacagccaga
     4561 cctaagacca gtacgatcaa tcctttaatt ttcatagtta cctccttgga tagataatat
     4621 aaagcatagt acgtgccaga tttcatgcat gtgcaattag agttattaaa cagactgtta
     4681 ttaacgattc aatattgagg agggcaagtg ccccgtgtat tctgggcggt ccaatgtgaa
     4741 tggcccccgg caatttgctc gtttgaaaac cagaacctgc aa
//
LOCUS       DASSLD010000593         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1121047, whole genome shotgun sequence.
ACCESSION   DASSLD010000593 DASSLD010000000
VERSION     DASSLD010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121047"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="VFK23_10715"
     CDS             <1..1238
                     /locus_tag="VFK23_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET7319598.1"
                     /translation="MEPDRLPLRDLMRYFLDFRLDIVTKRLNFDLRILKARLHILSAF
                     EALYDDLDTAIKLIRKSKSREEAADKLKKHFKFDDEQTRAILEMQLYRLAGLEIEKIL
                     KEKAEKSKQKKDIEAILASQKKIWALIKSELLEIKGNYGDKRKSVLKAAEDVEFSQED
                     FIVHEDVTVIMTKMGWIRRVKTVGENLRFKEGDELLALLPANTRDNIAVFSSAGKVYV
                     MKAFDLPTGSGFGEPVQHLFKFGDGERPIAAMNFATVMAEPAPSAGVQPELALGPREV
                     MALTVSAHGAGFAFDLGQYNAVTTRAGKKFAGVKEGDSVMGVAILDLPNVLFLTSEGK
                     AVVVAAKDIPVLTGAGKGVKLVNVKNGDVALFRAVRKNEQVKVIDDKGKEKVIDLKDY
                     GVMTRGSVGHKAVKAVKFA"
     gene            complement(1274..2395)
                     /locus_tag="VFK23_10720"
     CDS             complement(1274..2395)
                     /locus_tag="VFK23_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7319599.1"
                     /translation="MLDKADFLHNLFDTIPSFIFIVDHDVRIHHVNASASKLLDTNNE
                     SVLFMRGGEALHCIHSTETPEGCGRAPACMDCVIRNSVKRSFQGGLVQRETTKFMLLH
                     AAGTKEAHMMVTASPLRYGDREFCLLILEDVTKLKNAEEALRRHAAQLEAANQELESF
                     SYSVSHDLKAPLRGVLGFSEALLSDHLAGLNEEGRDYLERIHSAGLRMNQLIDDLLTL
                     SRVTKGEMRRETVDLSALAGAVADALVKSFPDRDVEFVVAHAVRVAGDGRLLRIVLEN
                     LMGNSVKFTTGREEARIEFGVQVQKGVPVYFVRDNGAGFDNASASRLFTPFQRLHEES
                     RFPGHGIGLATVRRIIHRHGGRIWAEGAVDQGASFFFTL"
BASE COUNT          714 a          846 c          885 g          617 t
ORIGIN      
        1 acatggagcc cgaccgcctg ccgctcaggg atttgatgcg ctatttcctc gatttccgac
       61 tcgacatcgt cacaaagcgg ttgaacttcg accttcgcat actcaaggcc cggctccata
      121 ttctctccgc gttcgaggcg ctctacgacg atctggacac cgccatcaag ctcattcgaa
      181 aatcgaaatc ccgggaggag gcggccgaca aactcaaaaa acacttcaag tttgacgacg
      241 aacagaccag ggcgatcctc gagatgcagc tctacaggct cgcgggcctc gagatcgaaa
      301 agatcctcaa ggaaaaggcc gagaagtcga aacagaagaa ggacatcgag gccatcctcg
      361 cctcccagaa aaagatctgg gcccttatca agtcagagct cctggagatc aagggaaatt
      421 acggagacaa gcgcaaatcc gtgctgaagg cggccgagga cgtggagttc tcgcaggagg
      481 attttatcgt ccacgaggac gtgactgtaa tcatgacgaa gatggggtgg atcaggcgcg
      541 tcaagaccgt aggggagaac ctccggttca aggaaggcga cgagctcctg gcgctcctgc
      601 cggcgaacac ccgggacaac atcgccgtgt tttcgtccgc gggcaaggtc tacgtcatga
      661 aggccttcga cctgcccacg ggctccggtt tcggcgagcc ggtgcagcac ctgttcaagt
      721 tcggagacgg cgagcggcct atcgcggcca tgaatttcgc gacggtgatg gcggagcccg
      781 caccctcggc gggagtgcag ccggagcttg cactcggccc gcgcgaggtg atggccctga
      841 ccgtttccgc ccacggagcc ggtttcgcct tcgacctggg ccaatacaat gccgtgacca
      901 cgcgggccgg aaaaaagttc gccggcgtca aagaaggaga ttccgtcatg ggcgtggcca
      961 tcctcgacct gccgaacgtc ctgttcctca cctccgaagg aaaggccgtc gtggtcgccg
     1021 cgaaggacat ccccgtcctg accggcgccg gcaagggcgt gaagctcgtt aacgtgaaaa
     1081 acggcgatgt cgccctcttc cgggccgtca ggaagaacga gcaggtgaag gtgatcgacg
     1141 acaagggcaa ggagaaggta atcgacctca aggattacgg cgtgatgact cgggggagcg
     1201 tgggacataa ggccgtgaag gccgtgaagt tcgcatagaa tttgaaaagg ttaatcacat
     1261 atttgtacag cagctacagg gtaaagaaga acgaggctcc ttgatccacc gccccctcgg
     1321 cccagatgcg gcccccgtgc cggtgaatga tgcggcgcac ggttgccaga ccgatgccgt
     1381 gtccggggaa ccgggactct tcgtggagcc gctgaaaggg cgtaaagagc ctgctcgcgg
     1441 aggcattgtc aaacccggcc ccgttgtccc tgacgaaata gacaggaacg cccttctgca
     1501 cctgaactcc aaactcgatc ctcgcctcct cgcggccggt cgtgaatttc accgagtttc
     1561 ccatgaggtt ctccaggacg atccgtaaca gcctgccgtc tcctgcgacg cggacagcgt
     1621 gcgcaacaac gaactcaacg tcgcggtccg gaaaggactt cacgagcgca tcggcaacag
     1681 cgcctgcgag cgcgctgaga tcgacagtct cgcgccgcat ctcgcccttc gtcactctgg
     1741 aaagagtcag caggtcgtcg ataagctggt tcatccggag gcccgcggaa tgaatgcgtt
     1801 cgaggtagtc cctgccctcc tcgttgaggc ccgcgaggtg gtcgctcaga agggcctcgc
     1861 tgaagcctag cacgcccctg agcggggcct tcagatcgtg cgaaaccgag taactgaagg
     1921 actccagctc ctggttcgcg gcctccagct gagccgcatg ccgccggagc gcctcttcgg
     1981 cgttcttgag cttggtaacg tcttccagga tgagcaggca gaattcccgg tccccgtatc
     2041 ggagggggct cgcggtcacc atcatatggg cttcctttgt cccggcagcg tggagcagca
     2101 taaacttcgt ggtctcacgc tgcaccaagc ctccctgaaa agacctcttg acggagttcc
     2161 tgatcacaca atccatgcac gcaggtgccc gtccgcaccc ttcgggcgtt tcggtcgaat
     2221 ggatgcaatg cagagcttca ccgccccgca tgaaaagaac cgattcattg ttggtgtcca
     2281 ggagtttcga tgcagatgcg ttcacgtgat ggatccggac atcgtgatcc acaatgaaga
     2341 tgaacgaggg aatcgtgtcg aagaggttgt ggagaaaatc cgctttgtct aacaagtcct
     2401 tggtcatggt aagcgaacgg ttaagaggcc gcggttcgct ttaaaataca acattcggcg
     2461 cttgtttgca agaggtgatg ttagcgccta tcttaaaccc cgttcgagct cattgacatg
     2521 aagccgagtc aggcatatat ttgtgtcaaa atgagcgatg cctatctaac agttggactc
     2581 caggcatcgc ttgatgaata tcataaaacg aatagaacgt gccatagtcg gtatttttgc
     2641 ttattgccag aagaaaacag aaattatctc gaataacacg ccggagactc ggaaaggcgg
     2701 ggtgtcattg aaatgaaaga gcatatcagt gacgattcta acaactcgcg tcggacacgt
     2761 cgcgaaaaac tgcgatccct acgcgaacgt aacacgatgc ggaatagacg gatgctgaaa
     2821 attcgcctca cggtgctggc ggtgtcgctg ctcggtgtcc tcgtcgctta tttttttctc
     2881 aaactatgga tgaatgctca tcctcgataa ggagccggct gcggaaaagt ctttattgtt
     2941 tgttaaactt tcagcgcgta gttcttcctg aagcgttgaa gttttcacga ttggaaacga
     3001 gtcatggggc aaatggtatt ggatcatcaa tgccagtatt aaggcgggtt ttatcatgaa
     3061 ga
//
LOCUS       DASSLD010000594         5735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_892940,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000594 DASSLD010000000
VERSION     DASSLD010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892940"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1633)
                     /locus_tag="VFK23_10725"
     CDS             complement(<1..1633)
                     /locus_tag="VFK23_10725"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7319600.1"
                     /translation="MTQTLAGGIKTVLAEENAFIREAHLSGAGGSEIVQRRTTLIDRT
                     LREAHEAHTRLAASGPMPSLVAIGGYGRGELNPYSDIDIMFLCRDEEDRKRAPELLYL
                     FWDAGLDVGYSVRTVKECVTLGRQDIRVRTSLLESRLIAGDRALYDEFLRTMRSEVFY
                     WKASEFIHEKVRERSETRRKYGGSIYLREPNIKEGEGGLRDFHTALWISFVHYRINSL
                     DELVAHGVISGGQFAVFQRSRNFLWRVRNEIHLLSGRKNDHLTFDLQERAAADFRFRD
                     SAHLLAVERFMKAYFLHARNIREFSNIVVDAVLRKPSPRWFDRKVTIGPFSLIGRTLV
                     SASDDLCRNDPSLVMSAFEVAQARHAVFSEHMKKLIRGCRIDDGVRTSDAASRAFLAI
                     LNNPDSLAETLTLMKDLRLLGRYLPEFRSIQALARHDYYHLYTVDEHILLAVRNLQDL
                     WTGSYPGLASLVDAFKGLKKRWVLILAVLLHDLGKAFRRDHERAGAELAGTVLDRIGV
                     RGEDRERVLFLVRHHLVMASLSQRRELTDRKVIADF"
     gene            complement(1798..2211)
                     /locus_tag="VFK23_10730"
     CDS             complement(1798..2211)
                     /locus_tag="VFK23_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319601.1"
                     /translation="MHQRTFRAVIILVAFILLCGMGGTGGFERAPRVEKNFTVTITDT
                     SGNKISGEKFSWEGRIHFAGYVGMAQVTMPFDRIQDLAMGEIRERKVRVTAHLTDGSE
                     TSFDVEARSRCFGEATFGSFMLQMDEIKSIVFNKK"
     gene            2466..3242
                     /gene="mazG"
                     /locus_tag="VFK23_10735"
     CDS             2466..3242
                     /gene="mazG"
                     /locus_tag="VFK23_10735"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HET7319602.1"
                     /translation="MNHTFQDLVALMARLRGPEGCPWDRKQTPASLKPFLVEECYEVI
                     DALDEDDPAKVKEELGDLLFQIIFHARIAEERGHFTIHDVIAANVEKMIRRHPHVFGD
                     ARLETDKEVLANWEELKKKEKGYEKRTSVLEGVPRHLPSLLRAHSLQERAARVGFDWS
                     RIDEALPKLDEEMAEFKESLKKEDPAGIEEELGDIFFMLVNISRFLGVNPEDALRKTI
                     SKFIRRFRHIEERAADASRSLGDMTLDEMERLWQESKGKE"
     gene            complement(3282..5381)
                     /locus_tag="VFK23_10740"
     CDS             complement(3282..5381)
                     /locus_tag="VFK23_10740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HET7319603.1"
                     /translation="MSAVLVILAVFPVVLFSSPVFAAPHITGAKPEPARMIGTNPRAR
                     LALTRARAMYVPAQFPADIRVIFLRVDFQPETDPAAFQVTGSGVWLDPLYSQGSGIPA
                     DANDLNDPSNKWVNKSRTDFIRYWSEASYGALTITVDISPVVYRLGWKMSHYGSESSA
                     ALENLIYDSVTAAAADANPATRIDFTLYDAILVVHAGVGEETDIGNSSNDIWSLYYDG
                     GGRICQNANALGPCLTTILRDGNVVGEGIIMPQTDSRDSAVVDPLGVYVHEFGHWLGL
                     PDLYCTGRACVLDGAGKWSLMADGIYNADPSSQSDPSNPATCADERKQCIFGSSPASL
                     DAWSRVFLGFAVPRTLDAGGGAGRMTLNPVENSAEIVKVQASSSVDSQYFLIENRQKT
                     GFDKGLPGHGMLVWLVDDVVVNRNFATNSINNSKARPGIKLMEADNDWKLLSYGCGNT
                     DDCGSAGDPFPGSSNNISFAPHTQPASRSYASFAWVNVRNIAENGEVVSADVGIGPLP
                     PPLPGMNENTVVWQQSAEPGVAGYTVYRNGILLEQTTSTSYTDVSCRNGDAYQVAAID
                     AQGNESDFSGVVTANMQVIAGRGDSRCFIATAAWGTPLDPHVGSLRNFRDRHLLTNAA
                     GRAFVAFYYRVSPPLAGFIGRHEGLRALTRNALTPVVYAVEYPRAAFLLCLIFTTALV
                     LLRQIWTGRMKRKSAAG"
BASE COUNT         1193 a         1694 c         1741 g         1107 t
ORIGIN      
        1 aaaaatcggc gatcaccttg cggtccgtga gctcgcggcg ctgcgatagt gaggccatca
       61 ccaggtggtg tctgacgaga aagaggaccc gttcgcggtc ttctccccgg acgccgatgc
      121 ggtccaggac tgtcccggcg agctccgccc ccgcccgttc gtgatcgcgg cggaaggcct
      181 tgcccaggtc gtggagcagc accgcgagga tcaggaccca tctctttttc aatcccttga
      241 acgcgtcgac gagggaggcc agcccgggat aactgcccgt ccagaggtcc tggaggttcc
      301 tgaccgcaag gaggatgtgt tcgtccacgg tgtacaggtg gtaataatcg tggcgcgcca
      361 gcgcctggat ggagcggaac tcgggcaggt agcggcccaa aagacgcagg tccttcatca
      421 gggtaagggt ctcggcgaga ctgtcgggat tgttaaggat ggcgagaaag gcccgcgagg
      481 cggcgtccga cgtcctgacc ccgtcgtcga tccggcagcc tcgaatcagt tttttcatgt
      541 gttcggagaa tacggcatgc ctcgcctggg cgacctcgaa ggccgacatg acaagggagg
      601 gatcgttccg gcacagatcg tccgacgcgc tcacgagcgt cctgccaatc agggagaagg
      661 gcccgatcgt caccttacgg tcaaaccagc gcggggacgg tttcctgagc accgcatcga
      721 cgacgatatt ggaaaactcg cggatgttgc gggcgtgcag aaaataagcc ttcatgaacc
      781 gctcgacagc caggaggtga gccgaatcgc ggaaccggaa atccgccgct gcgcgctcct
      841 gaaggtcgaa ggtcaggtgg tcgttcttcc ttcccgaaag caggtgtatc tcgttccgca
      901 cgcgccagag aaaattccgt gaccgctgga aaaccgcgaa ttgccctccg gagatgaccc
      961 cgtgagcaac gagttcatcg agcgaattga tccggtagtg gacgaaggat atccagaggg
     1021 ctgtatggaa gtcccgcagc ccgccttcgc cctccttgat attgggctcc cgcagataga
     1081 tggaaccgcc gtacttccgg cgcgtttcgc tccgttccct gactttttcg tggatgaatt
     1141 ctgatgcctt ccagtaaaaa acctcgctcc gcatggtccg gaggaactcg tcatagagcg
     1201 cgcgatcgcc cgcgatgagg cgggactcca gcagggaggt ccgtacccgg atgtcctggc
     1261 gcccaagggt cacgcactcc ttgacggtcc tgacgctgta gcccacgtcc aggccggcgt
     1321 cccagaacag gtagagcagc tccggtgccc gtttccggtc ctcctcatcc cggcacagga
     1381 acatgatatc gatgtcggaa taaggattca gctcccccct cccataaccc ccgatggcga
     1441 cgagcgacgg catggggccg gaggcggcaa gacgggtatg ggcttcatgg gcctcgcgga
     1501 gcgttctgtc gatgagagtg gtgcggcgct ggacgatctc gctcccgccg gcgccggaga
     1561 ggtgcgcctc cctgatgaag gcgttttcct cagcgagcac cgtcttgatg ccgccggcaa
     1621 gggtctgggt catattttga cgagacgcat tcacgaaaaa tcacgatgag ggaggtggga
     1681 gaaaagacgt tggacgacgg acaacgaaac ccgagcggcc tcttccctca tcctccgtca
     1741 tccccggtcc cgaatgtttt catggttccc tcatgttccc ctaagagtca gaagccctta
     1801 tttcttattg aacacgatcg acttgatctc gtccatctgg agcatgaagc tgccgaaggt
     1861 tgcctccccg aagcaacggc tcctcgcttc aacgtcgaaa ctcgtctcgc ttccgtcggt
     1921 gagatgcgcg gtaacgcgca ccttccgctc ccggatctcg cccatggcca gatcctggat
     1981 cctgtcgaag ggcatcgtta cctgcgccat gccgacgtat cctgcgaagt gtatccgacc
     2041 ctcccagctg aacttctccc ccgaaatctt gttgcccgag gtatcggtga ttgtgacggt
     2101 gaagttcttt tcgacgcggg gggcccgctc gaagcctccg gtgcctccca tgccacaaag
     2161 aaggataaac gccacgagga tgataaccgc ccggaatgtc cgctggtgca tagcaagtct
     2221 cctttcggga tcaatgatgc aggaatcatg aagttagatt accatacaac acgttggagt
     2281 ttcaagaaaa aagctcaaag gaaaggactt gcaatggctg gtctgattgt gtaaggtagg
     2341 ctgtaagttg cacgagggac gatggacgac cgtctcgctc ggacgacaga cgcggcacga
     2401 tgcaatcatt tccgtccctc gtctttcgtt cctcgtccat ccctgccgtt aaatgaggtg
     2461 ttcctatgaa ccatactttc caggatctcg tcgcgctcat ggcgagactc cggggccccg
     2521 aaggttgccc ctgggaccgc aagcagaccc ctgcatcgct caagcctttt ctcgtcgaag
     2581 aatgctatga ggtcatcgac gccctggacg aggacgaccc tgcaaaggtg aaggaggaac
     2641 tcggcgacct tctattccag atcattttcc acgcccgcat cgccgaggag cgcgggcact
     2701 tcaccatcca cgacgtgata gccgccaacg tggagaagat gatccggcgg catccccacg
     2761 ttttcggcga tgccaggctc gagacggaca aagaagtcct tgcgaactgg gaggagctca
     2821 agaaaaagga aaaggggtac gagaagcgca cgtcggtcct ggaaggcgtg ccgaggcatc
     2881 tgccgtccct cctccgcgcc cacagccttc aggaacgggc ggcgcgagtc ggtttcgact
     2941 ggagccggat cgacgaggcg ctccccaaac tcgacgagga gatggcggag ttcaaggagt
     3001 ctctgaaaaa agaggaccca gcgggaatcg aggaggaact gggcgacatc tttttcatgc
     3061 tcgtcaacat ctcgcggttc ctgggcgtca accccgaaga tgcactcagg aaaacaataa
     3121 gcaaattcat ccggcgattc cggcacatcg aggagcgagc cgccgacgcg agccggtcgc
     3181 tcggcgacat gacgctggat gaaatggagc ggctctggca ggaaagcaag gggaaagagt
     3241 aaaaaggacc aatcggggaa tcggggattg acgccggagc ctcagcctgc cgcactctta
     3301 cgcttcatac ggcctgtcca tatctgtcga agaagtacca gcgccgtcgt gaaaatcagg
     3361 cacaacagga aagccgcgcg cggatactct acggcataca cgaccggggt cagcgcattg
     3421 cgcgtgagtg cacgcagccc ttcgtgacgc ccgatgaacc ccgccagggg cggcgataca
     3481 cggtagtaga acgcgacaaa tgccctgcct gcggcattgg tcaggagatg ccggtccctg
     3541 aagtttctca gcgacccgac atgcgggtca agcggtgtac cccaggccgc cgtcgcgatg
     3601 aagcacctgc tgtcccccct gcccgcgatc acctgcatat tcgccgtcac gactccggaa
     3661 aaatccgatt cattgccttg cgcatcgatg gccgcgacct ggtatgcatc gccgttgcgg
     3721 catgagacgt cggtataaga cgtcgatgtc gtctgctcca gcaagattcc gttccgatac
     3781 acggtgtacc ccgccacacc gggttccgcg ctctgctgcc agaccacggt gttctcgttc
     3841 atgcccggca acgggggcgg cagcgggccg atccccacgt cggcgctcac gacctcgccg
     3901 ttctccgcga tgttcctgac gttcacccat gcaaaggacg cataggacct cgaggcaggc
     3961 tgtgtgtgcg gcgcaaagga gatgttgttc gaagagcccg ggaagggatc tcccgcgctt
     4021 ccgcagtcgt ccgtgttgcc gcagccgtag ctcaggagct tccagtcgtt gtcagcttcc
     4081 atcagcttga tgccgggccg ggccttgctg ttgttgatgc tgttcgtcgc gaaattgcgg
     4141 tttaccacca catcgtcgac gagccatacc agcattccat ggccgggaag acctttgtcg
     4201 aatcccgttt tctggcggtt ttcgatgagg aaatactgag agtcgaccga cgacgacgcc
     4261 tggaccttga ctatctcggc gctgttctcg acgggattca gcgtcatgcg gccagcgccg
     4321 cctccggcat ccagcgtccg cgggacggcg aagccgagaa ataccctgct ccaagcatcg
     4381 agggatgccg gggagcttcc gaaaatacat tgttttcttt cgtccgcgca ggtggcggga
     4441 ttggatgggt ccgactgact ggaggggtcg gcgttataaa ttccatcggc catcaggctc
     4501 catttgcctg cgccgtcaag gacacacgcc cggccggtac aatagaggtc gggaaggccc
     4561 agccagtggc cgaattcgtg cacatagacg ccgaggggat cgacgaccgc gctgtcgcgg
     4621 gaatcggtct ggggcatgat gatgccttca ccgacgacgt tgccgtcccg gaggatcgtc
     4681 gtgaggcagg ggcccagcgc gttggcattt tgacaaattc tgcccccgcc gtcgtaatac
     4741 agggaccaga tgtcatttga agaatttccg atgtccgttt cctcgccgac ccctgcatga
     4801 acgacgagga tggcatcata gagcgtgaaa tcgatcctgg tggcgggatt ggcgtcggcc
     4861 gcggctgccg ttacactgtc gtagatgagg ttttcgagcg cggcgctcga ttcactgccg
     4921 tagtgggaca tcttccagcc gagccgatag acgacgggag agatatcgac cgtaatggtc
     4981 agggcgccgt aggaggcctc actccagtac cggatgaaat ccgtcctgga cttgttgacc
     5041 catttgtttg acggatcatt cagatcgttg gcgtcggcgg ggatgccgga cccctgagag
     5101 taaagcggat cgagccacac gccgctcccc gtgacctgaa aagccgccgg gtccgtttcc
     5161 ggctggaaat cgacgcggag gaagatcacc ctgatatccg ccggaaattg cgccggcaca
     5221 tacatagccc tcgcacgggt cagtgcgagt cgcgcccgcg ggttcgtgcc gatcatcctg
     5281 gccggctccg gttttgcccc ggtgatatgc ggggctgcaa aaacgggaga ggaaaaaagg
     5341 acgacgggaa agactgccag gatgacgagg acggcagaca atttccaact cccccggcaa
     5401 cgcctgctcc cggagggatc gtgattcatg gttctcttgc ccaagatgat caggttcctt
     5461 ttttacgatg aagcggttgt aatgcggtca gtcgggaatg ccatggcttc cgggaccggt
     5521 cgaatacact atacgtcttc tcaaggtatt tttcaacagt ttccggggaa attgtcgggg
     5581 taaaacgacg gaacagacga ggactcgctg acgagtcgcc aggggagggt aaaaaagaag
     5641 ccggcgaggg gtcccccccg ccggccctta ccggggcatc ataatgcgca cagggaatcc
     5701 aggtctgtct gtgaatacac gaccggcgcg ccgcc
//
LOCUS       DASSLD010000595         2610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_693849,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000595 DASSLD010000000
VERSION     DASSLD010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693849"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VFK23_10745"
     CDS             <1..689
                     /locus_tag="VFK23_10745"
                     /inference="COORDINATES: protein motif:HMM:NF018201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7319604.1"
                     /translation="LLADMAGALAKAGRPRLNVSLDSLKAKRFRDITGQDGWERVWRG
                     INLSEELGFFPLKINMVPVRGMNDDEVVDFARLTLDRRLHVRFIEFMPIGASDRWHRD
                     VCVSSDQVRAAIERECGPLVPFAASPSAGPSVNYQVPGARGVIGFISPITRHFCDSCR
                     RLRLTADGKIRPCLLSDAEIDIKAALRSSCGNAELEGILRTALEQKPEHHTLAEAGGC
                     FQRTMSRIGG"
     gene            808..1305
                     /gene="moaC"
                     /locus_tag="VFK23_10750"
     CDS             808..1305
                     /gene="moaC"
                     /locus_tag="VFK23_10750"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HET7319605.1"
                     /translation="MYQPMSELTHLDDQGAARMVDVSGKEKTPREAVAEGRVTMLPET
                     LSLILDHKVPKGDVFAVARIAGIMAAKKTAELIPLCHPIALSSVEIDFEPDHAGSCVN
                     IRASAKTVDSTGVEMEALTAVAAAGLAIYDMCKSADRSMTLTEVRLMKKTGGKSGTFE
                     RKPGS"
     gene            1325..1867
                     /locus_tag="VFK23_10755"
     CDS             1325..1867
                     /locus_tag="VFK23_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET7319606.1"
                     /translation="MKPIGPLMIEHRLIEKIFVAVHEKARLAVEKKQIDPVFLDTAVD
                     FIRWYADKTHHGKEEAILFRDVAKKDMTAEHRRLMQELIDEHDFGRRTVGRLVEAREQ
                     YVRGEDQALGVILNITETLVHFYREHIRKEDKLLYPACMEYLDEQEQAAMLQEFGEAD
                     SKMIHAKYGAVVEQLEKTAQ"
     gene            1942..2199
                     /locus_tag="VFK23_10760"
     CDS             1942..2199
                     /locus_tag="VFK23_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319607.1"
                     /translation="MIEALREEEKKLRLVRFIVDLNQAILMQQADLTLREAFDILSNT
                     RKAVLNLFPDKESVFDLVYSPRFRRIIRERFVIPGGLAGPR"
BASE COUNT          574 a          742 c          741 g          553 t
ORIGIN      
        1 tgctcctggc agacatggcc ggagcgcttg cgaaggcagg ccggccgcgg ctcaacgtca
       61 gcctcgactc tctgaaggcg aagcggtttc gggatataac gggtcaggac gggtgggaga
      121 gggtttggcg cggcatcaac ctctcggaag agctcggttt cttcccgctc aagataaata
      181 tggtccccgt ccggggcatg aacgacgacg aggtagtcga tttcgcccgg ctcacgctcg
      241 accgccgtct gcacgtccgt ttcatcgagt ttatgcccat cggcgcgagc gatcgctggc
      301 accgggacgt gtgcgtatcc tccgaccagg ttcgcgccgc catcgaacgg gaatgcggtc
      361 ctctcgtccc ctttgccgcg tccccttctg caggcccttc ggtcaattac caggtgccgg
      421 gcgcgcgcgg ggttatcggg ttcatcagcc ccattaccag gcatttctgc gactcctgca
      481 ggcgcctgcg gttgacggca gacggcaaga tccggccctg tcttttgtcc gacgcagaga
      541 tcgatatcaa ggcggccctc cgcagttcct gcggcaatgc ggagctcgaa gggatcttaa
      601 ggaccgcgct cgaacagaaa ccggagcacc acacgcttgc tgaagccggg gggtgcttcc
      661 aaaggaccat gtcgaggatc gggggatagt tagagcaagc accaatctcc aaatcacaaa
      721 ttccaaataa tattcaaatt caaagctcta atgattggta cgtttggaat tttgaaattg
      781 acgtttgaaa tttatttggg atttggaatt tatcagccta tgtctgaact cactcacctc
      841 gatgaccagg gcgctgcgcg catggtcgac gtatcgggca aggaaaagac cccgcgcgag
      901 gcagtcgccg agggccgggt caccatgctc cccgaaacgc tttcgctcat tctcgaccac
      961 aaggttccga agggcgacgt ctttgcggtt gcccggatag cgggcatcat ggccgcgaag
     1021 aagaccgccg aactgatccc cctgtgccat ccgatcgccc tctcatccgt ggaaatcgac
     1081 ttcgagcccg accatgccgg atcgtgcgtg aacatccggg cctcggcgaa gacggtagac
     1141 agcaccggcg tcgagatgga ggcgctgacg gcggtggccg ccgccggcct tgcaatctac
     1201 gacatgtgca aatcggcgga ccgctccatg acgctcaccg aggtccgcct catgaaaaaa
     1261 acaggcggaa agagcgggac cttcgaacgg aaacccgggt cgtgactccc gaaggagatg
     1321 ttccatgaaa ccgatcggcc ccctgatgat cgaacaccgg ctcatcgaaa aaatcttcgt
     1381 ggcagtgcat gaaaaggcgc ggctcgcggt ggagaaaaaa cagatcgacc cggtgttcct
     1441 tgatacggcg gtcgacttca tccggtggta cgcggacaag actcatcacg ggaaagaaga
     1501 ggccatcctc ttccgcgatg tcgccaaaaa ggacatgacc gccgagcacc ggcgtctcat
     1561 gcaggagttg atagacgagc atgacttcgg caggaggacc gtcggccgac tcgtggaggc
     1621 cagggagcaa tacgtccggg gcgaagacca ggccttgggg gtaatcctga acatcacgga
     1681 gacgctcgtc cacttctacc gggagcatat tcggaaagaa gacaaactcc tctaccccgc
     1741 gtgcatggag tatttggatg aacaggaaca agcggccatg ctgcaggaat tcggggaagc
     1801 cgacagcaag atgatccacg cgaagtacgg ggccgttgtc gagcagctcg aaaagaccgc
     1861 gcagtaaacc tctccccttg aaaaaatccc ttcgccgaag tatcatactg tagttcgagt
     1921 ccgtttccag gaggaagctc tatgattgaa gcgcttcggg aagaagaaaa gaagctgcgg
     1981 ctcgtgcggt ttatcgtgga tttgaaccag gcgatcctta tgcagcaggc tgatcttact
     2041 ctccgggagg cgttcgacat cctgtcgaac acgaggaagg cggtgctgaa cctgttcccc
     2101 gacaaggaat ccgtgtttga cctggtttat tctccgcgct tcagaaggat catccgcgag
     2161 cggtttgtca tcccaggcgg cctcgcggga ccgagatgaa ggaccgaatc ggcgagaggg
     2221 caaaccggtg aacaggcgaa aaggcagaga gcttcatttc ctttcgcccg ctcatcgatt
     2281 cgcctttcga ttttttcctt cccgtttccc cgtttcatct ttttctttat cccgtttcgg
     2341 tttttgcact tctttccgcc ctgtttccgc ctcgttatga ccacaatgga atagcgcggc
     2401 ttgttcgata tttatccatc cagtttctcc atttcagtca cgacgtcccc aggaatttcc
     2461 tctgttcagg attcatttgc agagcagagc cctacggcgt ataatgtacg acctaagatc
     2521 ggctatatgc tcatatgctg aatctcaaac gaaaacattt ctggtgcgcg atcggtgccg
     2581 gttttccggc attcctgata ttcgtctcga
//
LOCUS       DASSLD010000596         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_404173,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000596 DASSLD010000000
VERSION     DASSLD010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404173"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFK23_10765"
     CDS             <1..290
                     /locus_tag="VFK23_10765"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET7319608.1"
                     /translation="DRCCGFGGVMRITHRGLSDGIAEEKARAIMATEASAVVTGCPGC
                     RMQIANALTRAGAEIEALHTVQVLDEALSNAECGIRIAELEFSGVRGNRRL"
     gene            340..987
                     /gene="fsa"
                     /locus_tag="VFK23_10770"
     CDS             340..987
                     /gene="fsa"
                     /locus_tag="VFK23_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017762792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HET7319609.1"
                     /translation="MKFFIDTANVKEIREAASLGIVDGVTTNPSLVAREGRDFKQVIA
                     EICSIVDGPISAEAVSLEADKMVAEGTELAKIHKNIVVKLPMTKEGLKATKALAALGI
                     NVNMTLVFSPMQALLAAKVGAAYVSPFAGRLDDISHYGMDLVRQIVAIYDNYGYGTEV
                     IVASVRNPLHVVDAALAGAHIATIPFGVIDQLVKHPLTDIGIEKFLSDWKKLEKK"
     gene            1054..1278
                     /locus_tag="VFK23_10775"
     CDS             1054..1278
                     /locus_tag="VFK23_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET7319610.1"
                     /translation="MPIYEYHCNACGADFEKLVFGSSPVVACEKCGSDKTEKLLSRFG
                     MGRSSNSSSSGSSGSSGCSGCSSSSCAGCH"
     gene            1563..>2105
                     /locus_tag="VFK23_10780"
     CDS             1563..>2105
                     /locus_tag="VFK23_10780"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HET7319611.1"
                     /translation="MTHMYLGNELLSCASLLPAGIYALLKKHKQDFLYGNIMADMILG
                     KKYLPDDKSSHSWDVGLKLLDQAKTWPERAFAYGYLSHLAADTVAHETLTDELGNVEH
                     TWAELKADSIIDKSYWLQTVTISKAVRKRSDLLLENSLDSYIFSIRTNKRIYKSMVFL
                     MFLNEKRRRGVDRALIHDLHE"
BASE COUNT          453 a          618 c          623 g          411 t
ORIGIN      
        1 ccgaccgctg ctgcggcttc ggaggcgtga tgaggatcac gcaccgcggt ctttccgacg
       61 gcattgcgga agaaaaggcc agggcaatca tggccacgga agcatcggcc gtcgtcacgg
      121 gctgcccggg ttgccgcatg cagatcgcaa acgccttgac gcgcgcgggt gctgaaatcg
      181 aggcgctcca tacggtgcag gtgctggatg aggcgctttc aaatgcggaa tgcggaattc
      241 ggattgcgga attggaattc tcgggcgttc gcggtaaccg caggctttag ccggggtgga
      301 acagattagc aacggcacat cggaaaggaa aaaacgagca tgaaattttt catcgatacg
      361 gcgaacgtca aggagatacg cgaggctgcg agcctgggta tcgtcgacgg ggtcacgacc
      421 aacccgtcgc ttgtcgcccg ggaaggccgc gatttcaaac aggtgatcgc cgagatctgc
      481 tccatcgtcg acggcccgat cagcgccgag gccgtgagcc tggaggcgga caagatggtc
      541 gccgaaggga cggaactcgc gaagatccac aagaacatcg tggtgaagct cccgatgacg
      601 aaggagggcc tcaaggccac gaaggcgctt gccgcactgg gcatcaacgt gaacatgacg
      661 ctggtcttct cgccgatgca ggcgctgctc gcggcaaagg tcggcgcggc ctatgtcagc
      721 cccttcgcgg gcaggctcga cgacatcagc cactacggca tggacctcgt ccgccagatc
      781 gtggccatct acgacaacta cggctacggg acagaggtga tcgtcgcgag tgtgcggaac
      841 ccgctccacg tcgtcgacgc ggcgctcgcg ggcgcccaca tcgccacgat ccccttcggc
      901 gtcatcgacc agctcgtcaa gcatccgctc acggacatcg ggatcgagaa gtttttatcg
      961 gactggaaga aactggagaa gaagtaaagg acgcggcgac gaggcgacga ggagcgtccc
     1021 cgtgtcaccc cgtctccgta tcaggtgcac actatgccta tttacgaata tcactgcaac
     1081 gcctgcgggg ccgatttcga gaagctcgtc ttcggttcga gtcccgtcgt cgcctgcgag
     1141 aagtgcggtt cagacaagac ggaaaaactc ctgtcccgct tcggcatggg ccggtcgtcg
     1201 aactcctcgt cctccggctc ttcggggagc agcggctgca gcggctgttc ttcgtcgtcc
     1261 tgcgccggat gtcattagca gggatatttg atagcggtgg ttgcgggatt ccggatgttg
     1321 aatcgtaaac gggattccct cctgaacgac cttacccggc agcgtattct gttatagccg
     1381 atcctcttac ttttgtattg acgcggggac ggagaagggg tatcatacaa taattcattt
     1441 gggttgccgg ggatcggatc atttcctccg gccttctata aatacaaaag cgcatgtcgt
     1501 ttatcctatt acttgcagcg ggaatcatct tcattccttc tttcgccttc gcctggggtc
     1561 cgatgaccca catgtatctc ggcaatgagc tgctgtcctg cgcctccctg cttcccgccg
     1621 gaatctacgc cctcctgaag aaacacaaac aggattttct ctacggcaat atcatggccg
     1681 acatgatcct cgggaaaaaa taccttcccg acgacaagag ctcccactcc tgggacgtgg
     1741 gcctcaagct cctcgaccag gccaagacat ggcccgaacg ggcctttgcc tacgggtatc
     1801 tttcccacct cgccgcggac accgtggcac acgagacgct tacggacgaa ctggggaacg
     1861 tggagcatac ctgggccgag ctcaaggcgg acagcatcat cgacaagtcc tactggctgc
     1921 agacggtcac gatcagcaag gccgtgcgca agcggagcga cctgctgctc gagaactctc
     1981 tcgacagtta catcttctcg atcaggacga acaagcgcat ctacaagagc atggtgttcc
     2041 tcatgttcct gaacgagaag agacgtcgag gagtcgaccg ggccctcatt cacgatctcc
     2101 acgag
//
LOCUS       DASSLD010000597         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1089828, whole genome shotgun sequence.
ACCESSION   DASSLD010000597 DASSLD010000000
VERSION     DASSLD010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089828"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            441..1688
                     /locus_tag="VFK23_10785"
     CDS             441..1688
                     /locus_tag="VFK23_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7319612.1"
                     /translation="MEFRQLDVPFEVKQGIENVGFTVLTPVQEAVIPRAVRGEDVAAQ
                     AQTGTGKTAAFLISLLSRMIRNPAGDLGNSPQALIIAPTRELADQIYHEGLRLGKFTG
                     FHLLAVYGGIDYQKQMDELRRGVDVLVGTPGRLIDYFKQNVYSLKKTRYLVIDEADRM
                     FDMGFIDDLRYLLKKMPPYAKRQSMLFSATLSYRVMELAYEFMNLPEKISITPEKITV
                     EQVEQVLYHVGKHEKIPLLLGILRKEQPARALVFVNTRRAADMVVERLNRNGWKADAI
                     TGDIVQKKRLRLLADFKEGKLPILVATDVASRGIHVEAVSHVINYDLPQDAEDYVHRI
                     GRTGRAGAAGKAISLADEEYVYSLEDIEKYIGQKIPVEWADEAMYVREIRRTAEEKQR
                     AASAPPRRHPSEKHGPRSRAKRR"
     gene            1874..>2576
                     /locus_tag="VFK23_10790"
     CDS             1874..>2576
                     /locus_tag="VFK23_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7319613.1"
                     /translation="MLSSLNIERPWLNFYEKGVPATLTYPELTIGDVLKETAKKFPLH
                     PALVFYGKKVTYAELDSLANRFANALIRLGVRKGDRVALMLPNIPQMVIAFYGTLKTD
                     AVAVPTNPLYHEHELEVQLNDSGAQTLVAVDMFYPVVSRVLPMTRVKNLILCRIKDFL
                     PFPLNLLYALKSGMEGQRVRVKRTPPIYDFMNLLKDAPSGQPVLAVSADDTAVLQYTG
                     GTTGVPKGAVLTHRNL"
BASE COUNT          551 a          810 c          718 g          497 t
ORIGIN      
        1 ccacaccaat taaaatatag actaaaaata attgttattc agccttattt cacatttttt
       61 ttatgaatag cggacggtcg ctgttgatgc atcgcgacgt gacgactgca tgcttgaacg
      121 cggcggggcc gttcattttt gccatcggga cgcgagtgac cgcataaaca gcgccctccg
      181 tcattcacgc gatcgaagtt ctccccttgc ggataagttc ctcacttccg gagcggccgc
      241 tctcgacgtc cccttccctt cgtcgtctcg ggaatacgaa gcgatccgcg ttgagataac
      301 ctccatcctt ccgcatcccg gcggtgcgcg catgtgcgca cggcagattt cgcttttgtt
      361 cgacgtacca cttatgctat actgaaaaaa atatttcgtt gacgtattgt ctgcataacg
      421 caaatctcca tcgaggaaaa atggaattcc gacagctgga tgtacctttc gaagtaaagc
      481 agggaatcga gaacgtgggc ttcaccgtgc tgacgcccgt tcaggaagcg gtgataccgc
      541 gggcggtgcg cggggaagat gtcgcggcgc aggcgcagac cggcacgggc aagacggcgg
      601 ccttcctgat ctcgctctta tcccgcatga tcaggaaccc ggccggcgac ctcgggaact
      661 ccccacaggc actgatcatc gcgcccacgc gcgagctcgc ggaccagatc taccacgagg
      721 gcctgcggct cggcaagttc acgggcttcc acctcctcgc cgtgtacggc ggcatcgatt
      781 accagaaaca gatggatgaa ctccggaggg gagtcgatgt gctggtcggc acgccgggca
      841 ggctcatcga ttatttcaag cagaacgtgt acagcctcaa gaagacccgg tacctggtca
      901 tcgacgaggc tgaccgcatg ttcgacatgg gattcatcga cgacctgcgg taccttctga
      961 agaagatgcc gccctacgcg aagaggcagt ccatgctgtt ctccgccacc ctctcctacc
     1021 gcgtgatgga gcttgcctac gaattcatga acctccctga aaaaatctcg atcaccccgg
     1081 agaagatcac cgtggagcag gtggagcagg tcctctacca cgtgggcaag cacgagaaga
     1141 tcccgctgct tctgggcatc ctccggaaag agcagccggc gcgggccctt gttttcgtga
     1201 atacccggcg cgcggcggac atggtcgtcg agcggttgaa ccgcaacggc tggaaggccg
     1261 acgcgatcac cggcgacatc gttcagaaaa aacggctcag gctccttgca gacttcaagg
     1321 agggaaagct tcccatcctc gtggccacag acgtcgcgag ccgcggcatc catgtcgaag
     1381 cggtctccca cgtcatcaac tacgacctcc cgcaggacgc ggaagactac gtgcaccgca
     1441 tcggccgcac aggccgcgcg ggggccgccg gcaaggcgat cagcctggct gacgaggaat
     1501 acgtttattc cctcgaggac atcgaaaaat atatcggcca gaagatcccc gttgaatggg
     1561 ccgatgaagc catgtacgtc agggagatcc ggcggaccgc cgaagaaaaa cagcgggccg
     1621 cctccgcgcc tccgcgccgc cacccctccg aaaaacacgg tccccggtcg agagcaaaga
     1681 ggcgctaaag ccccttccgg ccgcccaccc cgccccgcct gcacgggcct cgaaacgtta
     1741 cccgaccgaa cgactttacc ccggcgtgtt ataatagtac tagagatttc agagtatttc
     1801 agtctggaat tttccgggcc gttgcacgac ggtgacggcc gcggtccgga aggaggtctt
     1861 tcctttgact gccgtgctgt cctctctgaa catcgaaaga ccctggctga acttctacga
     1921 aaagggcgtg cctgcgaccc ttacctaccc cgagctgacg atcggggatg tgctcaagga
     1981 aaccgcgaag aagtttccgc tccatccggc gctcgtattt tacgggaaga aggtcaccta
     2041 cgctgaactt gactctctcg cgaaccggtt tgcaaacgcg cttatccgcc tcggtgtccg
     2101 caaaggcgac cgggtcgccc tcatgctgcc gaacattccg cagatggtca tcgcctttta
     2161 cggaacgctc aagaccgacg cggtcgccgt gccgacgaac cccctctacc acgagcatga
     2221 gctggaagtg cagctcaacg acagcggggc ccaaaccctc gtggcggttg acatgtttta
     2281 tcccgtcgtt tcccgcgtcc tgccgatgac gcgggtgaag aacctgatcc tgtgcaggat
     2341 caaggatttc ctccccttcc cgctcaacct cctctacgcc ttgaagtccg gaatggaggg
     2401 gcaacgggtc cgggtcaagc ggacgccgcc catctatgat ttcatgaacc ttctgaagga
     2461 tgctccgtca gggcaacccg ttcttgcggt gtccgccgac gacaccgcag tcctgcaata
     2521 caccggcggg accacgggcg tcccgaaggg tgcggtcctg acgcaccgca atctcg
//
LOCUS       DASSLD010000598         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_111654,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000598 DASSLD010000000
VERSION     DASSLD010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111654"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1830)
                     /gene="ppdK"
                     /locus_tag="VFK23_10795"
     CDS             complement(<1..1830)
                     /gene="ppdK"
                     /locus_tag="VFK23_10795"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET7319614.1"
                     /translation="MATGKKYIYSFGSGKADGTGTMKDLLGGKGAGLAEMTNAGIPVP
                     PGFTITTEVCNLFYELGKRVPDGLDTEMREYMKKVEAAVGSYRFGDVNKPLLVSVRSG
                     SKFSMPGMMDTVLNLGLNDQTIQGLIRLSSNERFAWDAYRRFIQMFGNVVMGIEKEVF
                     EHIIHSVKKKKKVHLDIDLTAADLMGLVESFKAKVKQVKKREFPQDPWEQVMMARDAV
                     FNSWNNPRAITYRRLNDIPGNLGTAVSVQAMVFGNMGDTSATGVGFTRNPSTGAKEFY
                     GEYLTNAQGEDVVAGIRTPHQIADLAKEMSPVYHQLRDITNRLEKHYRDVQDFEFTVQ
                     EGKLFMLQTRTGKRTAHAAVKIAVDMVAEGLISKEEAVLRVEPASLEQFLHPVIDPKA
                     KIEVIAKGLPASPGAASGKVVFTADEAVKHAKKDKVILVRPETTPDDIHGMDAARGIL
                     TARGGMTSHAAVVARGMGKPCVAGCESIKVDLKAEKFTVGKYTVKMGDYITIDGGTGR
                     VILGKVPTKDPEVSGDFGTLMNWVDEVRTLGVRANADIPRDAKMARKFGAEGIGLCRT
                     EHMFFAEDRLPIVQQMILADNTEAREKALSKLLPMQRSDFKGLF"
     gene            complement(1960..>2539)
                     /locus_tag="VFK23_10800"
     CDS             complement(1960..>2539)
                     /locus_tag="VFK23_10800"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HET7319615.1"
                     /translation="LVTEACKNYRLGNHDIISVAAETLDFLRQRLAGILASEGIRSDV
                     VDAALSVGFDDPLIGREKVRALDALRTSEDYQPLVTALKRAGNILPKEFAGTVNTNLL
                     KQGAEQSLYTAYMEIKDRVMEKAAKLDFRGALADIASLRKNVDAFFDTVMVMDKDTEV
                     KNNRLALLAGITGLFSGIADFSRLVLNTEESK"
BASE COUNT          513 a          757 c          662 g          607 t
ORIGIN      
        1 gaagaggccc ttgaagtccg agcgctgcat cggaagaagc ttggaaagcg ccttctcgcg
       61 cgcttcggta ttatcggcga ggatcatctg ctgcacgatc ggcagccggt cttcggcgaa
      121 gaacatgtgc tcggtgcggc agagaccgat gccctcggcg ccaaacttgc gagccatctt
      181 tgcatcgcgc gggatgtcag cattggcccg cacacccagg gtgcgtactt catcgaccca
      241 attcatgagc gtgccgaaat cgccgctcac ttccgggtcc tttgtcggaa ccttaccgag
      301 gattacgcgg cctgtaccgc catcgatcgt gatgtaatca cccatcttta cagtatattt
      361 cccgaccgtg aacttctctg ccttgaggtc aaccttgatg gattcgcagc cagccacgca
      421 gggtttaccc ataccacgcg ccacaacagc cgcgtgggat gtcatgccgc cgcgcgccgt
      481 gagaattcct cttgccgcgt ccatgccgtg aatgtcgtcg ggtgtggtct ccggtctaac
      541 gaggatgacc ttgtctttct tcgcgtgctt cacagcttcg tcggcggtaa agaccacttt
      601 tcccgatgcc gcgcccggcg atgccggaag gcccttagcg atcacctcga tcttcgcctt
      661 gggatcgatg acaggatgga gaaattgttc aagagaagca ggttcaacgc gcagcacggc
      721 ctcttccttg gagatgagcc cttcagcgac catatcaacg gcgatcttga ctgcagcatg
      781 ggccgtgcgc ttgcccgtac gggtctgcag catgaagagt ttgccttcct gcactgtgaa
      841 ctcgaaatcc tgaacgtcac ggtaatgctt ttcaagcctg ttcgtgatgt cgcgaagctg
      901 gtggtacaca ggcgacattt cctttgcaag atcggcaatc tgatgcggcg tccgaatgcc
      961 ggccactacg tcctcgccct gtgcattcgt caggtactcg ccgtaaaact ccttggcacc
     1021 cgttgaaggg ttcctcgtaa agcccacacc agtagctgag gtatcgccca tattgccgaa
     1081 caccatagcc tggacgctca cggccgtgcc gaggttgccc ggaatgtcat tcaatctacg
     1141 gtaggtaatg gcacgggggt tgttccagga gttaaagact gcgtcccgtg ccatcatgac
     1201 ctgttcccag gggtcctggg gaaactcgcg cttcttgacc tgcttaacct tggccttgaa
     1261 actttcaacg aggcccatca gatctgctgc cgtaagatcg atatcgaggt gcactttttt
     1321 cttcttcttc accgaatgga tgatgtgctc gaacacttcc ttctcgatgc ccatcaccac
     1381 attgccgaac atttggatga accggcgata ggcatcccag gcaaagcgct cattgctgga
     1441 caagcggatg aggccctgga tcgtctggtc gttcaggcca aggttcagaa ctgtgtccat
     1501 catgccgggc atcgagaact tcgatcccga acgaacggac acgaggagcg gcttgttcac
     1561 atcaccgaag cgatagctgc caaccgcggc ctcgaccttc ttcatgtact cgcgcatctc
     1621 agtgtcgagg ccgtccggga ctcgcttgcc gagttcatag aacagattgc agacctcggt
     1681 cgtgatggtg aaccccggcg ggaccggtat gccggcgttc gtcatctcgg caaggcctgc
     1741 gcccttgccg ccgagcaggt ccttcatcgt tcctgtccca tcggcctttc cgctgccgaa
     1801 agagtaaata tatttcttcc ccgtagccat gtgactatcc tccctggaaa agttttagga
     1861 cttagcttta acttatatat acaggaatca gatttgagga gagctttatt tatccttctc
     1921 cttcgacata tctaatctct gtcttttttt aaacttcgat tatttcgact cttccgtgtt
     1981 taacaccagc cgcgagaaat ccgcgatgcc ggaaaaaagc ccggtgattc ccgcaagtag
     2041 cgccaagcgg ttattcttca cctcggtgtc cttgtccatg accatgactg tatcgaaaaa
     2101 agcgtcaacg ttcttcctga gagaagcgat gtcggcaagt gcaccccgga aatcgagctt
     2161 cgccgccttt tccatcaccc gatccttgat ctccatataa gcggtataga ggctctgttc
     2221 cgctccctgt ttcaggaggt tcgtgttgac cgtgcccgcg aattctttcg gaaggatatt
     2281 gcctgcccgc ttgagcgctg taaccagcgg ttggtaatct tccgatgtcc gcagggcatc
     2341 gagcgccctc accttttccc gtccgatgag cggatcgtcg aacccgacgg acaacgcagc
     2401 atccaccaca tcggagcgga tgccctccga tgcaaggatc cccgccaggc gctgccgcag
     2461 gaagtccagc gtctccgcgg caacgctgat gatatcgtga ttcccgagcc ggtagttctt
     2521 gcatgcctcc gtgaccagt
//
LOCUS       DASSLD010000599         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1055058, whole genome shotgun sequence.
ACCESSION   DASSLD010000599 DASSLD010000000
VERSION     DASSLD010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055058"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..827)
                     /locus_tag="VFK23_10805"
     CDS             complement(12..827)
                     /locus_tag="VFK23_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET7319616.1"
                     /translation="MTRHLRIGVMVFTAAFIASGCVSSGTFKKMEAGKNQEIATLQQE
                     KASLEQTRKSLDQQNAELKQQVSSLDQQKATLEKQKTELEQEKAAAQQQVGSLEQQKA
                     ALLANSQQRQQQYEELVQGLSKEVEKGQLQVRQYQNMLAVDLAEQIFFDSGRASLKAG
                     GKDVLKKVAGALKGYENKIIRVVGHTDNVPVAKSLQGIFPSNWELSVARATNVVRFLQ
                     VSGIPPERMVPSGRAEYDPVAPNDTPEGRQKNRRIEIMLIDKSLVSEMTKTGM"
     gene            complement(891..1364)
                     /locus_tag="VFK23_10810"
     CDS             complement(891..1364)
                     /locus_tag="VFK23_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319617.1"
                     /translation="MKAYKMMFTGLMVFLLASSPAFAGDFDWLQKLNIRAEADPSGFR
                     AELATRFKIGNTEINAVIGNMAKPADAYMVLRLAEMSHQPTDRVIAQYRSGRGQGWGV
                     LAKSLGIKPGSPEFHALKRGHDLYGSNDSSMDKGRGKGRGRNHAALERKEVNYTV"
     gene            complement(1447..>2364)
                     /locus_tag="VFK23_10815"
     CDS             complement(1447..>2364)
                     /locus_tag="VFK23_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319618.1"
                     /translation="LNPRRIKRITADVSPRNEKDPALAYTLSLLFWGSGQLYNGMRTK
                     GLVYACLALYGNALAILLFTFQEELPLYLRFFSISPAQAMLAAELLLFLFLFFWISNA
                     SDAYHTAIKARRTPFTGVSGRAWPMICSLFVPGWGQFLNGQPVKGGLFAVIAAFGIFS
                     LASVMGILSVWHFLEDSSYRLIVEGVLLLSLFSLLPMPFLWALGSFDAWKVAQDDIKK
                     EPLLERLRAANNRRRDQGWVRGVFPQIKRTLLLAAILTILAAVIARYYLPWSYYREYV
                     VDTMRLSAKQGMVLIPEFLRRIIAVLPAK"
BASE COUNT          500 a          694 c          628 g          542 t
ORIGIN      
        1 aggaatcggt atcacatgcc tgttttggtc atttcactca ccaggctctt gtcgatcagc
       61 atgatctcga tccggcggtt cttctggcgg ccttccggcg tgtcgttcgg cgctacagga
      121 tcgtattctg cccgtccgga cgggaccatc cgctcgggcg ggatgccgga tacctgcagg
      181 aagcgaacga cattagtcgc ccgggcgacc gaaagttccc agttcgaggg gaagatgccc
      241 tggagtgact tggccaccgg cacattgtcc gtatggccca cgacccggat gatcttgttc
      301 tcgtacccct tcagggctcc ggcaaccttc ttcaatacat ccttgccgcc tgccttaagc
      361 gacgcccggc cggagtcgaa gaagatctgc tcagcaaggt ccactgccag catgttctga
      421 tactgccgca cttgaagctg gcctttttcc acttccttcg acagcccctg caccaactct
      481 tcatactgct gctgccgttg ctggctgttt gcgagaagag ctgccttctg ctgctccagt
      541 gacccgacct gctgctgcgc tgcggccttc tcctgttcca gctcggtctt ctgtttttcc
      601 agcgtggcct tttgctgatc cagtgacgag acctgctgtt tcagctctgc gttctgctga
      661 tccaatgatt ttctcgtctg ttccagggag gccttttcct gctgcaaggt cgcgatctcc
      721 tggttcttcc ctgcttccat cttcttgaac gtaccggaac tgacgcaacc gctcgcgatg
      781 aacgctgcag taaaaaccat aacaccgatg cggagatgtc tcgtcatatg caagcccccc
      841 ctttgacctt tcagtattat catgtcccca tcaacagcgt caggatgact tcaaacggta
      901 taattcactt cttttcgttc cagcgctgcg tgatttcgtc cacggccttt ccctctgccc
      961 ttatccatac tgctgtcgtt gctgccgtac agatcgtgcc cccgctttag agcgtggaat
     1021 tccggtgatc ccggcttgat gcccaggctc ttcgcgagca caccccaccc ttggcctctg
     1081 ccggaccggt attgcgcgat gacgcgatcc gtcggctgat gcgacatttc tgcgagacgg
     1141 agcaccatgt atgcgtccgc aggtttagcc atgttgccga taacggcatt gatctccgtg
     1201 ttaccaatct tgaaacgcgt cgccagctcc gccctgaatc ccgagggatc cgcttcggcc
     1261 cggatgttga gtttctggag ccagtcaaaa tcacctgcga acgctggaga cgaagcgagc
     1321 aggaacacca tcaaacccgt gaacatcatc ttgtatgctt tcatggaaac ctcctcgtag
     1381 cttatcagcc gattttactt cacatcaaat cttgatcgac atatcctctt gcccttcagg
     1441 actaatctat ttcgccggca ataccgcgat gattctcctg aggaactcgg ggatgagaac
     1501 catcccctgt ttcgccgaca accgcatagt atccacgacg tattctcgat aataactcca
     1561 gggtaaatag taccgcgcga tgacagccgc tagaatggta aggatagcgg caagcaggag
     1621 cgttctcttg atttgcggga agacgccgcg aacccatccc tggtcccggc ggcggttgtt
     1681 cgccgccctc agccgttcga gaagcggctc cttcttgatg tcgtcctggg ccaccttcca
     1741 ggcgtcgaat gagccgaggg cccagaggaa aggcatgggc agcagggaga acagggaaag
     1801 caggagcacc ccttcgacga tgagacggta agatgagtcc tcaaggaaat gccagacact
     1861 caggattccc atgaccgagg ccagggagaa aatgccgaat gccgcgatga ccgcgaacag
     1921 accgcctttg accggctgac cgttcaggaa ctggccccag ccgggtacaa acagggaaca
     1981 gatcatgggc caggcacggc cggacacgcc ggtgaagggc gttcttctgg ccttgattgc
     2041 ggtgtggtag gcatcactcg cattggagat ccagaagaac aggaataaga aaagcaggag
     2101 ctcggcagca agcattgcct gggccgggga gatgctgaaa aaacgaagat agagcgggag
     2161 ttcttcctgg aacgtgaaca gaaggatcgc aagtgcatta ccgtacaacg cgaggcaggc
     2221 gtagacaaga cctttggtgc gcattccgtt atacaattgt cccgaacccc agaacaggag
     2281 agaaagcgta tatgccaggg cagggtcctt ttcattgcga ggagatacat ctgccgtaat
     2341 tcgctttatc ctgcgaggat tcag
//
LOCUS       DASSLD010000600         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1014799, whole genome shotgun sequence.
ACCESSION   DASSLD010000600 DASSLD010000000
VERSION     DASSLD010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014799"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..423
                     /gene="trxA"
                     /locus_tag="VFK23_10820"
     CDS             100..423
                     /gene="trxA"
                     /locus_tag="VFK23_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET7319619.1"
                     /translation="MADAVTSATWEQEVLKAPGLVLVDFWAVRCGPCRMVAPIVDEIS
                     KEYEGKLKVLKLNTDENPDVAGKYRIMGIPTLMFFKSGQTVDQVVGAVPKAQLKTKVD
                     ALLAK"
     gene            560..1048
                     /locus_tag="VFK23_10825"
     CDS             560..1048
                     /locus_tag="VFK23_10825"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET7319620.1"
                     /translation="MVKRILAALILIMSILSGCTNRDVPHGGSTATEFTLPDLNGNKV
                     KLSDYKGKVVLLEFWATWCPPCRASIPGIEKLHETYKNKGLAVLAVSLDEGGWDTVKS
                     FVQKNGITYLVLRGNEEVAEKYQVRTIPLILILDKDGKISKRYLGFGNEEDLEKDIKA
                     VL"
     gene            1215..1290
                     /locus_tag="VFK23_10830"
     tRNA            1215..1290
                     /locus_tag="VFK23_10830"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1248..1250,aa:Gly,seq:ccc)
     gene            1478..1999
                     /gene="cobU"
                     /locus_tag="VFK23_10835"
     CDS             1478..1999
                     /gene="cobU"
                     /locus_tag="VFK23_10835"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HET7319621.1"
                     /translation="MDNRIYFVTGGARSGKSAHAERLARGLGGKHAYIATAQALDAEM
                     AARIEKHRQVRGDAWDTFEEPLAIAELVTKLSGRYQVVLLDCLTLWLSNVMAHTSGDG
                     DVLSRSDEFIKAIRKFPGACIIVSNEVGLGIVPDNPLARKFRDLAGMMNQKVAEAADE
                     VYFTAAGIPVKIK"
BASE COUNT          469 a          570 c          655 g          435 t
ORIGIN      
        1 attgcggggc atgcccccgg acgtgtccgg aaggcgcaat caaaatgaga atgttgcccc
       61 aaccggcagc gacgacaaaa taacttcgag gaggctggca tggctgacgc cgtaacgagt
      121 gcaacatggg aacaggaagt attgaaagcc ccgggtctgg tcctggtgga tttctgggcg
      181 gtcaggtgcg gcccctgcag aatggtggcg cccatcgtgg acgagatttc gaaagaatac
      241 gagggcaagc tcaaggtcct gaagctgaac accgacgaga accccgacgt ggccggcaaa
      301 taccgcatca tgggaatccc gacgcttatg ttcttcaaga gcggacagac cgtcgaccag
      361 gtcgtggggg ccgtgcccaa ggcgcagctc aagaccaagg tcgatgcctt gcttgcgaag
      421 taagattgcc gctgctaaag ttgccttttt ttcttcaggg ccgggtccgg ctaggggccc
      481 ggcctcgatt ttggcgctgc ccgtctccgg cgggcagttt tcattttgtc cggcgccgtc
      541 cgtcaaggag accgttttaa tggtgaaaag aattctcgcc gcattgatcc tgatcatgtc
      601 gatcctgtcc ggctgcacga acagggacgt cccccacggc ggctccacgg ctacggaatt
      661 taccttgccg gaccttaacg ggaacaaagt caagctttcg gattacaagg gcaaggtggt
      721 gctgctcgag ttctgggcca cctggtgtcc gccctgccgc gcatcgatac cagggataga
      781 gaaactccac gagacataca agaacaaggg cttggcggtc ctcgctgttt cgctcgacga
      841 gggcggctgg gatacggtaa agtctttcgt ccagaaaaac ggcatcacct accttgtgct
      901 caggggcaac gaagaggtgg ccgagaaata tcaggtgcgc acgatccccc tgatcctgat
      961 cctcgacaag gacggcaaga tctcgaaacg gtatctcggc ttcgggaacg aggaagacct
     1021 ggagaaggac atcaaggcgg tcctgtaagt tcttttctcc ctctccaaag ataaagaccc
     1081 atcccctccc ttgttccctc cccttgaagg ggaggggcag gggtggggat gcgtttgttc
     1141 ttcgggaggg tagggggagg aggggttttt tgattgacac cgcctctacc tgtcgttata
     1201 atgaccttcg aaaagcgggc gtagctcagc tggtagagta caagcttccc aagcttgggg
     1261 tcgcgggttc gagacccgtc gcccgctcca ttattccgaa aaaaaccggt attgccgctg
     1321 taacgaagct gcaaagcatt tcatgaataa ctgtgtaagc gctgtttccc tgctgattat
     1381 ttcttcgtgc ctcggcgtct ttgcggcctc gttggtgatc ttaccccctg cctggttcaa
     1441 taggtagggg ttttttgtcg cagatcgatg aaggaacatg gataacagga tttactttgt
     1501 tacgggcggc gcgcgttccg gaaagtcggc ccatgccgag agactggccc gcggcctcgg
     1561 aggaaaacac gcctacatcg ccacggcgca ggcgctcgac gcggagatgg cggcgaggat
     1621 cgaaaagcac cggcaggtcc ggggcgatgc atgggacacc ttcgaagagc ctctggccat
     1681 tgcggagctt gtaacaaagc tttcgggcag gtaccaggtc gtgctgctcg actgtctgac
     1741 gctctggctt tccaacgtca tggcgcacac gagcggcgac ggcgacgtcc tgtcccgttc
     1801 ggacgagttc ataaaggcga tcaggaagtt tcccggggcc tgcatcatcg tatcgaacga
     1861 agtgggactc gggatcgttc cggacaatcc tctcgcgagg aagttccggg accttgccgg
     1921 catgatgaac cagaaggtcg cagaggccgc cgacgaagtg tattttacgg cggcgggaat
     1981 accggtgaag ataaaataac gccgggaaac ggggttcggc aatgaagccc gtttcggggg
     2041 tcggggatgg acgaggaacg aacatacgaa cgacgcaaca gctcatctca cgttctcggt
     2101 gacgtgtccg agccgtcaaa aaaacgacc
//
LOCUS       DASSLD010000601         3866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1014531, whole genome shotgun sequence.
ACCESSION   DASSLD010000601 DASSLD010000000
VERSION     DASSLD010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014531"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2306)
                     /locus_tag="VFK23_10840"
     CDS             complement(<1..2306)
                     /locus_tag="VFK23_10840"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HET7319622.1"
                     /translation="MKKILSLIAILFVLLVAAVGVYVQSDAFSERIKPFVLGPLQAVL
                     GPDARVGRIKANFLPPYLDVRDIMIPDARGNEAVSVRRIKVYLNPVPLLVKKVQLPSI
                     SLIEPRINLERAADGTFNITPLVERIRQSLAKPEREEPAFSLLLKTISVRQGEIRFKD
                     EGLSSRATITGLDMTARFNVAKDHISVNLRSSRITFSAPAYPEIAGTLKAAGEYDRGL
                     VRVKTIDLEAGDTALNVSGTMGLLPESGLDLKGKLRSGPQSLGRLAQLFNPGQKRQTP
                     RVEASVTVTGKNSDPQVSGNVRFSGFAYRGLTLKNVEVSIRYRDHVLAVSGEKGKTDI
                     YGKALIIDRVTADLAYRDGVLDVRRAEIASGDLAVRMEGIVGQTRGFDAGLAVESSGE
                     GKTLSLLTGMPVEGGVAMKGRLTGALNAPLVEGTIEAGPVVVRRVQFDHVAGILKYRD
                     RVISIESADIRRQSSSYVFDGSADFKGGEPSYAARLKVAQSDVAGIVALFYEPLPLKI
                     TATGELSFRWNATNYSGSGSLSLDAGSAYGESFTRGVVTAELTKGKISFPEFVLYKKK
                     GMARATGWIGFDGTYSADLEISGVGLSSVDHMAGTDLDGDANLRLHTAGSFSTPVLNA
                     TFAVEKFSFRGTGMGTLRGSADIKDHALKAEAGLPEDAASLTLDWMLRKPYTWTAEAK
                     VKTDGFNPLLLFGNAALAERVKLIAAGGIRARGAGLDRSSVSGDASFSRIAVVIGEYR
                     IDSESRPAFTISRGRLAAKSLDFVGPSTR"
     gene            complement(2317..>3866)
                     /locus_tag="VFK23_10845"
     CDS             complement(2317..>3866)
                     /locus_tag="VFK23_10845"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7319623.1"
                     /translation="LCRNDPSLVMSAFEIAQARHAVFSEHMKKLIRGCRIDDGVRTSD
                     AAARAFLAILNNPDSLAETLTLMKDLRFLGRYLPEFRSIQALARHDYYHLYTVDEHIL
                     LAVRNLQDLWTGSYPGLASLVDAFKGLKKRWVLILAVLLHDLGKAFRHDHERAGAELA
                     GTVLDRIGVRGEDRERVLFLVRHHLVMASLSQRRELTDRKVIADFSRLVRDRENLAML
                     YLLTYADISAVNPGAWTQWKAALLQDVYMRTLNYIEKSAIVVEEDKARLASQAEKIRS
                     AASRLFRPAEVEALLADMPDQYILYTPTGKVLDHLAMMKRLPEEQLVIHHRHHPDRGY
                     TELTVCAYDAYGMFYRTAGAIASKNLNILRAQVYTSKSGVMLDTFQITDADGNLCQYE
                     AEWDSVLAGLRSALMSKARPPEPGPYAQLPKRASGVTPTVEFDNDTSEAFTIIDITAQ
                     DRVGFLYQVTKALYELNLDIGSAKIVTEGSKVMDSFYVTDLLHGKISEEQRLQKIKEM
                     LLAVLEG"
BASE COUNT          717 a         1190 c         1190 g          769 t
ORIGIN      
        1 cgggtggagg ggccgacgaa atcaagggac ttggccgcca gcctgcccct gcttatggta
       61 aacgcgggcc gcgattcgct gtcaatgcga tattcgccga tgaccacggc gatgcgggaa
      121 aaggacgcat cgcccgacac ggaagagcgg tcgagcccgg cgcctcgggc cctgatgccg
      181 cccgcggcga tcagtttcac ccgctcggca agcgcggcgt tgccgaaaag gaggagcgga
      241 ttgaaaccat cggtcttcac cttagcctcc gctgtccatg tgtagggctt ccggagcatc
      301 cagtcgaggg tcaggcttgc ggcgtcctcc ggcagccccg cctcggcctt cagcgcgtgg
      361 tctttgatat cggcactgcc gcggagcgtc cccatcccgg tcccgcgaaa ggaaaacttc
      421 tcaacagcga acgtcgcgtt cagcacgggc gtagagaagc ttccggcggt atgcagccgc
      481 aggtttgcat ccccgtccag gtcagtcccg gccatatggt ccacggatga tagaccgacg
      541 ccgctgattt cgaggtccgc cgaataggtc ccgtcgaacc cgatccaccc cgtcgccctg
      601 gccatcccct tcttcttgta cagcacaaac tcggggaacg agattttgcc cttcgtgagc
      661 tcggccgtta cgacgccgcg cgtgaatgat tctccgtaag cactgcccgc gtccagggaa
      721 aggctaccgc ttcccgaata gttcgttgcg ttccaccgga acgacagctc gcccgtcgcg
      781 gtgatcttaa gcggcagggg ttcgtaaaac agggccacga tgcccgcgac atccgattgg
      841 gcgaccttga gccgcgcggc ataggacggt tcccccccct tgaagtccgc agacccgtcg
      901 aacacatagc tcgatgactg ccgccggata tccgcagact cgatcgatat gaccctgtca
      961 cggtacttca gtattcctgc aacgtggtcg aactgaacgc ggcgcacaac gaccggcccg
     1021 gcctcgatcg ttccctcgac gagaggagcg ttcagcgcgc cggtcaatcg gcctttcatg
     1081 gcgacgcccc cctcgacggg catgccggtg aggagtgaga gcgttttccc ctcgcctgac
     1141 gattcgacag caagcccggc gtcgaaaccc ctcgtctgac caacgatccc ctccatcctg
     1201 acggcgaggt ctcccgatgc gatctcagct cttcttacgt cgaggacgcc gtcgcgatag
     1261 gcaagatcgg cagtcacccg gtcgatgatc agggctttcc cataaatgtc cgtcttccct
     1321 ttttctccgc tgacggcaag gacgtgatcg cggtaccgga tcgatacttc cacgttcttc
     1381 agcgttagcc cccggtacgc gaagcccgaa aacctgacgt tgccgctcac ctgcgggtcg
     1441 gaattcttcc cggtgaccgt gacggaggcc tccacccggg gggtctgccg tttctggccg
     1501 ggattgaata actgggccag tctgccgagc gactgcggcc cggaacggag ctttcccttc
     1561 agatcaagcc cggattcggg caggaggccc atcgttccgc tgacattgag cgctgtatca
     1621 ccggcctcga gatcgattgt tttcacgcgg accagccccc ggtcgtattc tcccgcggcc
     1681 ttgagcgttc ccgctatttc aggataggca ggagcggaga acgtaatccg tgagcttcgc
     1741 aggttgaccg agatatggtc cttggcgacg ttgaagcgcg cagtcatgtc caggccggtt
     1801 atcgtggccc gggaagacag tccctcatcc ttgaaccgga tctcgccctg cctcaccgag
     1861 atggtcttga gaaggaggct gaatgccggc tcttcccttt ccggctttgc gagggactga
     1921 cgtatccgct cgacgagcgg cgtgatgttg aaggttccgt cggcagcgcg ctccaggttg
     1981 atgcgaggct cgatgaggga gatggaggga agctgtacct tcttcacgag caagggcacg
     2041 gggttcaggt agaccttgat cctgcggacc gacaccgcct cgttgccgcg ggcgtccggg
     2101 atcatgatgt cccgtacgtc gagatagggc ggcagaaagt tcgccttgat gcgtccgacc
     2161 cgagcgtcag gtccgaggac tgcctgcagc ggccccagga caaacggttt gatccgctcg
     2221 ctaaaggcgt cgctctgaac gtaaacaccg acggccgcga ccagcaggac gaaaaggatc
     2281 gctatgaggg agaggatctt tttcataaat atgccgtcac ccttccagca ccgcgagcag
     2341 catctctttg attttctgaa gcctctgctc ctctgatatt ttaccatgca agaggtctgt
     2401 gacgtaaaaa gaatccatga cctttgaccc ctcggtcacg atcttcgcgg agccgatgtc
     2461 caggttgagt tcgtagagcg ccttggtcac ctggtagaga aaaccgacgc ggtcttgcgc
     2521 cgtaatgtcg atgatcgtaa aggcttccga cgtatcgttg tcgaactcca cggtcggcgt
     2581 gaccccggag gcccgtttcg gaagttgcgc atagggtccg ggttcgggcg gcctggcctt
     2641 gctcatgagt gccgatcgga ggccggcgag cacgctgtcc cactcggcct cgtactggca
     2701 gaggttcccg tctgcgtcgg tgatctggaa ggtgtcgagc atgacgccgc tcttcgacgt
     2761 gtagacctgg gcacggagga tgttcagatt ctttgacgcg atcgcgccgg cggtccggta
     2821 aaacatgccg taggcgtcat aggcgcagac cgtgagctcc gtatagcccc tgtcggggtg
     2881 atggcggtga tgaatcacca actgctcttc cggaagccgc ttcatcatcg ccaggtggtc
     2941 gaggaccttg cccgtcggtg tgtacaggat gtactggtcc ggcatgtccg cgagcaaggc
     3001 ttcgacttcg gcgggtctga acagccgcga cgccgcggac cttatcttct ccgcctggga
     3061 tgcaagcctg gccttgtcct cctcgacgac aatagcgctc ttttctatgt aattgagcgt
     3121 cctcatgtag acgtcctgga gcagcgcggc cttccactgg gtccaggcgc cgggattcac
     3181 cgccgaaatg tcggcatagg tcaggagata gagcatcgcg aggttctccc ggtcccgcac
     3241 gagccgggag aaatcggcga tcactttgcg gtccgtgagc tcgcggcgct gcgacaggga
     3301 agccatcacc aggtggtgtc tgacgagaaa gaggacccgt tcgcggtcct ctccccggac
     3361 gccgatgcgg tccaggacgg tcccggcgag ttccgccccc gccctttcgt gatcgtggcg
     3421 gaaggccttg cccaggtcat ggagcagcac cgcgaggatc aggacccatc tctttttcaa
     3481 tcccttgaac gcgtcgacga gggaggcaag cccgggataa ctgcccgtcc agagatcctg
     3541 gaggttcctg accgcaagaa ggatgtgctc gtccacggtg tacaggtggt aataatcgtg
     3601 gcgcgccagc gcctggatgg agcggaactc gggcaggtag cggcccaaaa aacgaaggtc
     3661 cttcatcagg gtaagcgtct ccgcgagact gtcggggttg ttgaggatgg cgagaaaggc
     3721 ccgcgcagcg gcgtccgacg tcctgacccc gtcgtcgatc cggcagccgc ggatcagttt
     3781 tttcatgtgt tcggagaata cggcatgcct cgcctgggcg atctcgaaag ccgacatgac
     3841 gagagaggga tcgttccggc acagat
//
LOCUS       DASSLD010000602         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_534538,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000602 DASSLD010000000
VERSION     DASSLD010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534538"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /gene="prfB"
                     /locus_tag="VFK23_10850"
     CDS             join(<1..39,41..1096)
                     /gene="prfB"
                     /locus_tag="VFK23_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139800744.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET7319624.1"
                     /translation="NLNGRLETLRRHLDTPAKESRLEELTRLTETTGFWDSKDKANKV
                     LKEKAALERALRDWNTLARRLSDLEAMAELASEEGGEAMRGELERELEAIGKDIASAE
                     VAALLSGEQDASNAIVSIHPGAGGTEAQDWAEMLMRMYLRWAEQRGYKTEILDYQAGE
                     EAGVKSVTFTVSGDYAYGRMRAEAGVHRLVRISPFDANKRRHTSFASVYIYPEVQEDI
                     DIEIDEKDLRIDTYRASSAGGQHVNKTDSAVRITHLPTNIVVQCQNERSQLKNKNVAM
                     KVLRSRLYELRKKEQEEKLGKLVSEKKDIAWGSQIRSYVFQPYQMVKDHRTGVEIGNV
                     TSVMDGDLDQFIEAYLISDSGARSQEPAAS"
     gene            complement(1223..>2495)
                     /locus_tag="VFK23_10855"
     CDS             complement(1223..>2495)
                     /locus_tag="VFK23_10855"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET7319625.1"
                     /translation="REPEIRRKPGPVIEKAGKATPVAVVKSIRYWSEGAYSRIVIDQS
                     GAVRFQAQELKNPDRLVFDLLHARAGASLSKEPQQINDGILKQVRASQHAPGVVRVVL
                     DLASLKSYAAFPLHDPERLVIDVTGEGGKISSAPPASENRAEQPAENRPTDAVPMVEP
                     PRQAPPIPVMPEVKDNEGAKLSLSNQLGLKIQTIAIDAGHGGHDPGAIGRSGLKEKTI
                     TLDIAKRLAALVQERLGCRVVMTRDRDVFIPLDQRPFIAKSHDADLFVSIHVNANRKS
                     RARGIETYIQSLRASDREAMATAARENAMSTKKLSELKTEVEKILEALDRDNKLEDSL
                     QLAHAVQGSLFDTMRPAQPRIINLGVKRAFFYVLMNTQMPSILAEVGFISNPEEEKLL
                     RNDAYRQKIAEALYQGVKRFIDARSPQMAGL"
BASE COUNT          491 a          771 c          752 g          481 t
ORIGIN      
        1 aacctcaacg gcaggctcga aaccctgcgg aggcatcttt gacaccccgg ccaaggaatc
       61 ccgcctggaa gaactgaccc gcctcacgga aaccaccggt ttctgggaca gcaaggacaa
      121 agccaacaag gtcttaaagg aaaaggcagc cctcgagcgc gcgctcaggg actggaacac
      181 gcttgcccgg cgcctgagcg acctcgaggc catggcggag ctggcctccg aggagggcgg
      241 cgaagccatg cgcggcgaac tggagcgcga gctcgaagca atcgggaagg acatcgcgtc
      301 cgccgaggtc gcggcgctcc tctccggaga gcaggatgcc tcgaacgcca tcgtatcgat
      361 tcatcccggg gcgggcggca ccgaggccca ggactgggcc gagatgctga tgcgcatgta
      421 cctgcgctgg gccgagcagc gcgggtacaa gaccgaaatt ctcgactacc aggccggtga
      481 ggaagcgggg gtgaagtctg tcaccttcac cgtgtccggc gattacgcct acggcaggat
      541 gagggcagag gcgggcgtgc accgtctcgt gcgaatctcc ccgttcgacg cgaacaaacg
      601 caggcacacg tccttcgcct ccgtctatat ctatcccgag gtccaggagg acatcgacat
      661 cgagatcgat gagaaggacc tccgcatcga cacctatcgc gcgtcgagcg ccggcggaca
      721 gcacgtgaac aagacggatt ctgctgtgcg cataacacac ctgcccacga acatcgtcgt
      781 gcagtgccaa aacgagcgct cccagctcaa gaacaagaat gtcgcgatga aggtgctgcg
      841 gtcgaggctg tatgaactcc ggaaaaaaga gcaggaagag aagctcggga aactcgtctc
      901 cgagaagaag gacatcgcct ggggaagcca gatcagatcg tacgtgttcc agccctacca
      961 gatggtgaag gaccaccgga cgggagtcga gatcgggaat gtcacgtccg tgatggacgg
     1021 ggacctggac cagttcatcg aggcatactt aatttcagac agcggagcca ggagccagga
     1081 gccggcagcc agctagactc aggaatgtgc ccgccgcata cgttcaaggc atcggctgga
     1141 aaatatccct gccgtttctt tattccgcat tccgaacgga gcctgccctc gaaagatcca
     1201 atcgggggca atccgccttc gattataacc ccgccatctg cggcgaccgc gcgtcaataa
     1261 atctctttac gccttgatac agggcttcgg ctattttctg ccggtatgcg tcgttgcgca
     1321 ggagtttttc ctcctccgga ttgctgatga aaccgacctc cgcaagaatg ctcggcatct
     1381 gcgtgttcat caggacataa aaaaacgccc gcttgacccc caggttgatg atacgcggct
     1441 gggccggcct catcgtatca aagagcgaac cctgtacggc atgcgcaagc tgcagcgaat
     1501 cctccagctt attgtcccgg tcgagcgctt caaggatctt ttcgacctct gttttcaatt
     1561 cgctcagctt cttcgtggac atcgcgtttt cccgggcggc ggtcgccatg gcctcccggt
     1621 ccgatgcccg aaggctctgg atataggttt cgatcccccg ggccctgctc ttccggtttg
     1681 cattgacatg gatggagacg aacaggtccg cgtcgtggga tttcgctatg aacggcctct
     1741 ggtcgagcgg aatgaacacg tccctgtccc tggtcatgac gaccctgcat ccgagcctct
     1801 cctgcacgag ggcggcgagc cgcttcgcta tgtcgagcgt tatggttttt tctttcagcc
     1861 cgctccttcc gattgctccc gggtcatggc cgccgtggcc ggcgtcgata gcgatggttt
     1921 gtatcttgag ccccagctgg ttggaaaggg acaatttcgc cccctcgttg tccttcacct
     1981 ccggcatcac cgggatcggc ggggcctggc gcggcggttc taccatgggg acggcatccg
     2041 tcggcctgtt ttctgccggc tgctctgcgc ggttttcgga cgccggcggg gccgaggaga
     2101 tcttgccgcc ttcgccggtt acatcgatga cgagacgttc ggggtcgtgg agcgggaaag
     2161 cggcataact cttgaggctc gcgaggtcga gcaccacccg gacgacgccc ggcgcatgct
     2221 gactcgcgcg cacctgtttc aggatgccgt cattgatctg ctgcggctcc ttgctcaggg
     2281 aagcgccggc gcgggcatgc aaaaggtcaa agaccagacg gtccggattc ttcagttcct
     2341 gtgcctggaa cctcaccgcg ccgctctggt cgatcacgat gcgggaataa gccccttcgg
     2401 accagtagcg gatgctcttc accacggcga ccggcgtcgc ttttccggct ttctcgatca
     2461 ccggccccgg cttcctcctg atctcaggct cccgc
//
LOCUS       DASSLD010000603         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1382578, whole genome shotgun sequence.
ACCESSION   DASSLD010000603 DASSLD010000000
VERSION     DASSLD010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382578"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFK23_10860"
     CDS             <1..492
                     /locus_tag="VFK23_10860"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7319626.1"
                     /translation="KGPAEQGQLLFFVGGKKADIDEARPLFEAMGKAVHHMGVHGMGT
                     SMKMVNNIMLSQAMIAFSEALTLGESLGIPKGLLLDTLLSSPVTAPFLALKRPKVEGG
                     AFDTEFPLQWAHKDLQLAADTAYETGVAMPLANVAKELYALAVKNGLGEQDFSAVYKL
                     LSK"
     gene            507..1040
                     /locus_tag="VFK23_10865"
     CDS             507..1040
                     /locus_tag="VFK23_10865"
                     /inference="COORDINATES: protein motif:HMM:NF033823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HET7319627.1"
                     /translation="MDSILVMPFGEVDRNALDFLARALRETYQCDTFVEDRAAVPDKT
                     CNIRRKQYNSTRILKAMEKIKHRTDELILGVIDADLYVPGLNFVFGEADVLARIAVIG
                     LPRLRQEYYGLEPDRDLFLLRAAKEAIHELGHTRGVGHCSDRSCIMHFSNSLPDTDAK
                     EPAFCTACRNNLEAGRS"
     gene            1157..1954
                     /locus_tag="VFK23_10870"
     CDS             1157..1954
                     /locus_tag="VFK23_10870"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HET7319628.1"
                     /translation="MIKPNLVSSSIQLASSHVDQMRGILDFFSSIYRDKIIIAEAACH
                     DTREAFKNFGYLRLLGEYNVELVDLNTGPSESYPITGHQNAIISVKLSRLLLDKGNYV
                     ISAAKLKTHDTVVVTLSIKNMAVGSIIGQDKKNVHQGVGQTNRIIAGLAGRVRIDLAV
                     IDGFEGMEGDGPTCGDPVYPGLGIASIDALAADRVACEVMGVNFHDIGYLHYCASQGV
                     GEADLNRIEVLGERLRDCIRPFKLHRSANEQYAWKQYEKQKCDKGNK"
     gene            complement(2011..>2278)
                     /locus_tag="VFK23_10875"
     CDS             complement(2011..>2278)
                     /locus_tag="VFK23_10875"
                     /inference="COORDINATES: protein motif:HMM:NF018439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HET7319629.1"
                     /translation="PGRIQFGLEYEMPLWLGKNQIGWYGAADFSATEERDWRLDRSLQ
                     AGLVTHRAGRTWRFGIEWYNGRPPLGEFFQYTEEYVSLGLWIDI"
BASE COUNT          528 a          637 c          646 g          467 t
ORIGIN      
        1 aagggtcccg cagaacaggg acagctcctg ttcttcgtcg gcgggaaaaa ggccgatatc
       61 gatgaagcgc gtcccttgtt cgaggccatg ggcaaggccg ttcatcatat gggcgtacac
      121 ggcatgggca cttccatgaa gatggtgaac aacatcatgc tgtcccaggc catgatcgcc
      181 ttttccgaag ccttgaccct cggcgaatcg ctcgggatac cgaagggact tcttttggac
      241 acgctcctca gttctcccgt tacggccccc ttcctcgcgc tcaagagacc gaaggtcgaa
      301 ggcggtgcgt tcgacaccga atttccgctc cagtgggcgc acaaggacct gcaacttgcc
      361 gcggacacgg cgtacgagac gggcgttgcc atgcccctgg ccaacgtggc aaaagaactt
      421 tatgccctcg cggtgaagaa cggtctggga gagcaggact tctcggcggt gtacaagctt
      481 ttgagcaagt gaggaagtat cctcccatgg atagtatcct cgtcatgccc ttcggggagg
      541 tggaccgtaa cgccctcgac tttctcgccc gtgctctacg ggaaacctac caatgcgata
      601 ccttcgtgga agaccgggca gctgtgccgg ataagacctg caatatccgg agaaaacagt
      661 acaactccac gcgcatactg aaagcaatgg agaaaatcaa acaccgaacc gacgagttaa
      721 tcctcggcgt gatcgatgca gacctctatg tcccggggct caacttcgtg ttcggagaag
      781 ccgacgtgct ggcccggatc gccgtcatcg ggctcccgag actgcgtcag gaatactacg
      841 gcctcgagcc ggaccgggat ctgttcctcc tgcgtgccgc gaaagaggcg atccacgagc
      901 tgggccacac ccgcggcgtc ggccactgtt ctgaccggtc gtgcatcatg catttctcga
      961 acagcctccc ggatacggac gcgaaggagc cggcattctg caccgcgtgt cgaaacaacc
     1021 tggaggccgg gagaagttga gtgtattcat gccgcgcgta tcattagtaa aaggcgatga
     1081 caggagaaca attgcgagga ggtcccttga cctcattgcc gatgacatta cacggggatt
     1141 aggatccaga cgacctgtga tcaaacccaa cctggtttcc tcgtcgatcc aactcgcatc
     1201 gagtcacgtg gatcaaatgc ggggcattct tgattttttt tcgagcatct accgcgacaa
     1261 aatcatcatt gcggaggctg cgtgccacga cacgcgggag gcattcaaga acttcgggta
     1321 tctgcggctt cttggcgaat acaatgtcga gcttgtcgac ttgaacacgg gtccctcaga
     1381 gtcctatccg atcaccggcc atcagaacgc gataatttct gtgaagctct cccgtctgct
     1441 gctggataaa gggaattacg tcatctctgc ggccaaattg aagacgcacg ataccgtagt
     1501 cgttaccttg tccattaaaa acatggccgt ggggagcatc atagggcaag acaaaaaaaa
     1561 cgtgcaccag ggagtcgggc agacaaaccg cattattgca gggcttgcgg ggcgagtgcg
     1621 gatcgacctg gcggtgatcg acggatttga gggcatggaa ggtgacggtc cgacatgcgg
     1681 cgatccggtt tatccggggc tggggatcgc aagcatcgac gctctggcgg cagaccgggt
     1741 agcctgtgag gtcatgggcg taaactttca tgacatcgga tacctgcatt actgcgcttc
     1801 gcagggtgtg ggtgaagccg atctgaacag gatcgaggtc ctgggagaac gtctcaggga
     1861 ctgcataagg cctttcaagc tccatcggtc ggcgaacgaa caatatgcat ggaagcagta
     1921 tgagaaacag aaatgcgaca agggaaataa ataaggtgta accagatgct tggcgttatt
     1981 gccggagaca taatcattgt cccccttgta ttaaatatcg atccacaaac ccagactgac
     2041 gtattcttcc gtatactgga aaaactcgcc gagcggcggc cggccgttat accactcgat
     2101 gccgaagcgc cacgttctcc cggccctatg cgttaccagg cccgcttgca gtgagcgatc
     2161 caggcgccag tctcgttctt ccgtcgccga gaaatccgcg gctccgtacc agccgatctg
     2221 attcttcccc agccacaggg gcatttcata ttccagcccg aactgaatcc tgccgggc
//
LOCUS       DASSLD010000604         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1570562, whole genome shotgun sequence.
ACCESSION   DASSLD010000604 DASSLD010000000
VERSION     DASSLD010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570562"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..728)
                     /locus_tag="VFK23_10880"
     CDS             complement(96..728)
                     /locus_tag="VFK23_10880"
                     /inference="COORDINATES: protein motif:HMM:NF017816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HET7319630.1"
                     /translation="MKKIAFCLLLLTISTIASAAEDKIVLANVDKNLFRQKPAAVAPV
                     VTEKYEYYEVRGNCEKDSQCRVRRKGIAWSDGKKYDSATSWHVTWKYDYNRTPDACSA
                     DSFRVFVEITFLYPRWVPGGDTTGDLTDRWEAYIKNLVTHENGHRDLAVEAAAELARA
                     VAELPPSPTCADVDREVRALSRVRMDMLNDDEKEYDTATNHGYTQGAVFR"
     gene            1104..2306
                     /locus_tag="VFK23_10885"
     CDS             1104..2306
                     /locus_tag="VFK23_10885"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET7319631.1"
                     /translation="MIVIEPFKGKSVIDQRVEIVERKGTGHPDYLCDAMMEAISVALS
                     REYLREFGTILHYNIDKGLLAAGRTEKRFGGGKVLKPMELTIGDRATFTAGGKKVPVA
                     DIAVDAAKGWLRENMRFLDPEKHLNYRVVLAPGSEELTDIFARPGVVRSANDTSAAVG
                     YYPLSPTEKIVLGLERHLNSRPFKKRHPDTGEDIKVMGLRTGKELDLTVAMPLISPFI
                     GSEREYFERKELLYREMQRFVAGSEDFRSVRVHFNTLDEPGRGLGGAYLSLLGTSAED
                     ADSGQVGRGNRVNGLISVSRPMGTEAAAGKNPVSHVGKIYNVFSHRIAEKLYKEIDGL
                     REVYVYLVSRIGAAIDRPLIAAAQIMPRPGLKIGDVRQRTEEIIEREFSRINRFCREL
                     SEGKYPVC"
     gene            2429..2944
                     /locus_tag="VFK23_10890"
     CDS             2429..2944
                     /locus_tag="VFK23_10890"
                     /inference="COORDINATES: protein motif:HMM:NF016356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HET7319632.1"
                     /translation="MSEKIKIGISSCLLGNNVRYDGGHKLDLSLRDDLGLIVEWVPVC
                     PEVESGLGVPREPMQLNGDPAAPRLIAIETKTDRTDDLMRLIEKKLPQLEQQGIRGFV
                     FKARSPSCGVHDTPLLTLTGGIAGVRAGLFAEAVIRCFPAMPVEDEERLRDPVAREDF
                     IKRIKKAVNHG"
BASE COUNT          657 a          799 c          881 g          633 t
ORIGIN      
        1 gttgcaaatg ttgagagcct cccttagagt tgagaaatgc atccgcaatc gtgaagcagg
       61 cagagtagcc tgcttttttg ttgttcaggc agcggtcaac ggaacacggc cccctgcgtg
      121 taaccatggt tggtcgccgt gtcgtactcc ttttcatcgt cattcaacat atccatacgc
      181 acgcgggaca gcgcccgcac ctcacggtcg acgtcggcgc aggtgggtga agggggcaat
      241 tctgccacag cgcgggcaag ttcagccgcc gcctccacag cgaggtcccg gtggccgttt
      301 tcgtgagtga caaggttttt tatgtaggct tcccacctat ccgtgaggtc cccggtggta
      361 tcgccgcccg gcacccatct cggatagaga aaggttattt cgacgaatac ccggaaggag
      421 tcggctgaac aggcatccgg ggtgcggtta taatcgtact tccacgtcac atgccagctc
      481 gtcgcagaat cgtacttttt cccgtcgctc cacgcaattc ccttccgcct caccctgcat
      541 tgcgaatctt tttcacagtt cccgcgcacc tcgtagtatt catatttttc ggtcacgacc
      601 ggcgccacgg ctgcgggttt ctgccggaac aggtttttgt cgacatttgc aagaacaatc
      661 ttgtcttcag cagctgatgc aatcgtggaa atggtaagaa gcaggaggca aaaggcgatt
      721 tttttcatca aggttgggag tcctccgatg attggacctc ttcctggcta ttccagcgga
      781 aagttctcag aacacccggg atgtatagct cctcgcgagg ggcaggtgtg gaagaaacag
      841 cgcagaggca atttctcgcc tgcaaatttt ctgaatatta cgacaaacgg catctcaatg
      901 caatagagaa aaaggcgttt tacgccgctc aaaaaatcga caagacgggc agggccgagc
      961 tgataaatgc gtataccggg cttctatacg aacaggcagg ctctcgcaga cgtccgcttc
     1021 gctcgatctg caaccggctt ggaatggcgt ccaaaaattg attttgattg cagcccgctt
     1081 ccgcactatg ataaactgtt ttcatgatcg tcattgaacc tttcaaggga aaatcggtca
     1141 tcgaccagcg ggtcgaaatc gtcgagcgca aggggaccgg acaccccgac tatctttgcg
     1201 atgccatgat ggaagccatt tccgtcgccc tctcccggga gtatctgcgg gaattcggga
     1261 cgatcctcca ctacaatatc gacaagggtc ttcttgccgc cggcagaacg gagaagcggt
     1321 tcggcggggg aaaggtcctg aagcccatgg agctgaccat cggcgaccgg gccacgttca
     1381 ctgccggcgg gaagaaagtc cccgttgcgg acatcgccgt ggatgcggca aagggttggc
     1441 tcagggagaa catgcggttc ctggatcccg agaagcatct caattaccgc gtcgttctgg
     1501 cgcccgggtc cgaagagctc acggacattt ttgcccgtcc cggcgtggtc aggagcgcga
     1561 acgacacctc tgctgcggtg gggtattatc ccctgagccc gacggagaag atcgtccttg
     1621 ggctggagcg tcacctgaac tcccggccgt tcaaaaagag gcaccccgat acgggggaag
     1681 acatcaaggt catgggcctg cggacgggaa aagaactcga cctcaccgtt gccatgccgc
     1741 tcatcagccc cttcatcgga tcggagcggg agtatttcga acggaaagag ctgctttacc
     1801 gcgagatgca gcggtttgtg gcgggctcag aggatttcag gagcgtccgc gttcatttca
     1861 ataccctcga cgagcccggc aggggactgg gcggagcgta tctcagcctg ctcggcacct
     1921 cggccgagga cgcggattcg gggcaggtgg gacgcggcaa ccgcgtgaac ggactgatct
     1981 cggtcagcag gcccatgggc accgaggcgg cggccggaaa aaatcccgtg agtcacgtcg
     2041 gtaagattta caatgtattt tctcatcgca tcgcggagaa gctctacaaa gagatcgatg
     2101 ggctgcgcga ggtatacgtc tatctcgtca gccggatagg cgctgccatc gatcggccat
     2161 tgattgccgc ggcgcagatc atgccccggc ccggtttaaa gataggggac gtgagacaac
     2221 ggaccgaaga gatcatcgaa cgggaattct ccaggatcaa caggttctgc agagaactga
     2281 gcgagggcaa gtatccggtg tgttgaggtc ggaagcattt taccagggat ggacaccgtt
     2341 tgacacagac tgagagcaag agataagaat ttgatcttgt atccgtggtc ttccgccgtt
     2401 gtccgtggta aacaggtttg tctttgatat gtctgaaaag atcaaaatcg gcattagctc
     2461 ctgcctcctg ggtaataacg tccggtacga cggagggcac aaactcgatc tttctctgcg
     2521 ggacgatctg ggccttattg tcgaatgggt gccggtctgt cccgaagtgg aatccgggct
     2581 cggcgtgccg agggaaccga tgcaactcaa cggcgatccg gcggcaccgc gactgatcgc
     2641 catcgagacg aaaacggacc ggacggatga tcttatgcgg ttgatcgaga agaaattacc
     2701 gcaactggag cagcaaggca tccgtggttt tgtgttcaag gcccgctccc cgagctgcgg
     2761 tgtccacgac acgccgctcc ttacgttgac agggggaatc gcgggtgtgc gcgccggcct
     2821 gttcgcggaa gctgtgatcc ggtgttttcc cgccatgcct gtcgaggatg aggagagact
     2881 gcgcgatccc gttgcgcggg aggacttcat taaaaggatc aaaaaagcgg ttaaccacgg
     2941 atgaccacgg atgattgtat ccgactgctg
//
LOCUS       DASSLD010000605         5383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1349752, whole genome shotgun sequence.
ACCESSION   DASSLD010000605 DASSLD010000000
VERSION     DASSLD010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349752"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFK23_10895"
     CDS             <1..279
                     /locus_tag="VFK23_10895"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HET7319633.1"
                     /translation="RFLYVGNTVSNDISAYVVDANTGALTPTPGSPFPAGLVPLDLTA
                     GPSDRFLYVVNNGSNDISVYSIDADTGGLSQVQTITSGGAAISIAITE"
     gene            305..751
                     /locus_tag="VFK23_10900"
     CDS             305..751
                     /locus_tag="VFK23_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319634.1"
                     /translation="MKKEKDLLWTWLLPEKSRTFDRALWVRRGGKWIVFDKGDAIAAL
                     AERLAPYIDSGEIESAKYWNGDPSAINVYSLDEDRGRVRSILKEAGARHVLVWEYDYA
                     MGKNFLRPVTFAYSWFSKFRTIFASYGLSGSIRLLKEVLSQRRGEG"
     gene            895..3081
                     /locus_tag="VFK23_10905"
     CDS             895..3081
                     /locus_tag="VFK23_10905"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HET7319635.1"
                     /translation="MPKVNFIFAIHNHQPVGNFEFVAEEAYQKSYLPFVRTLERHRQI
                     KITLHYTGILYRFFEQRHPEFIVLLKDLVAEGRAEILSGGFYEPVIAVLPDNDKIGQI
                     RALSEYVRNKIGYDAQGMWLAERVWEPHLPKPIADAGINHVVVDDFHFKMAGLRDDEL
                     DGYYLTEEQDRIVRIFPGSERLRYLIPFHGPEETISFLSGLRSTERNRLAVMADDGEK
                     FGVWPETYRTVYEEGWLERFFSLLEQNSDWLETTTFAEYLGREPARGRVYLPTSSYME
                     MGEWALPARAMKEYEDALEKVKNSPEFAGLRPFIKGGTWRNFLAKYSESNHMHKRMQM
                     TSRKVHAALDVPGTRKNADAARMLDHLYQSQCNDAYWHGIFGGLYLPHLRSGVYEQLI
                     RAEYLADKALKGRGTRDEGRGEKQKNQTASWLDIEQGDFDLDGNDELMLNSELLNIFI
                     DPAEGGRISELDWKPRMFNLANTLTRRREGYHDKIIRAAAAKTDAAQAGGVKTIHERV
                     AVKEEGLQYHLLYDWYTRGSLIDHFLGSGVDLAAFIRCEYYEAGDFVLGNYGAARKKA
                     GGSAAVILERVGNVAGLAVRLRKEISLRRSAPEFPVRYEIENLGAEELNTSFGSEFNF
                     SFLAGSAHDRYYDIPGHTLDRRNLASSGETNNVREVSLVDEWLKLRLTLSFDEPAMLW
                     RAPVETVSQSEAGFERVYQSSLIMPVWRISLGPGKSWNAEIRVRVE"
     gene            3255..5027
                     /locus_tag="VFK23_10910"
     CDS             3255..5027
                     /locus_tag="VFK23_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319636.1"
                     /translation="MKKLILLFLLIAVAFGTGAAASETPLGVEEIESVDELATGILSY
                     FPRVQGEVKAVNGERITVTLGTKDGIAPGVVLTLWRDSKEILHPVTGKVIGRTEDEVG
                     DAQVTDVMETACTAVMRKRLKDPKEGDKARITPKKISMALLPLRADRPETLQGLAERL
                     SESGRFSLLDSGTVAGFLRDRKERDRSLLKEMGKAYNLDVIAAVGIYPTEANKLLVTV
                     RLFYADEARPIDTIVAMLDLKSRKEAIAEVKPFFAPVTAEKNDVTALTFDAQLIAAAD
                     LEGSGSLQYAFSDGVKLHIYRQGAAGWREDWVEHVPAGAGEIQTINLDAADINGNGRS
                     ELFVTIMQSGKVVSRVFEFQEGAYHQTAEIPGFLRVITYPGMGTMLIGRAYDPVKFFS
                     GPVRQYQWSNGAYTAGAEMALPEDVGLYGFVVASLGEKAPLLVALNDEDRLRVYSGET
                     PIWKSEESYPSVGTSVMKPTTGIGAVLSQETEVVDKSRKVRIPGRVIALDLNGDGRDE
                     ILLPKNSGGTILRGHTQAEFVSLGWTGARLEQKWAIKDVGGAVVDFQVLRQEATGARI
                     LALVRSSGGLFSRDTIKVVSYPAK"
     gene            5106..>5383
                     /gene="uppS"
                     /locus_tag="VFK23_10915"
     CDS             5106..>5383
                     /gene="uppS"
                     /locus_tag="VFK23_10915"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET7319637.1"
                     /translation="MNKKSPSREEQLDKAGLPRHVAIIMDGNGRWAMRKGLPRIAGHK
                     RGVDTVRRVTEECRRIGIEVLTLYAFSDENWGRPKEEVGFLMDLLGTFL"
BASE COUNT         1170 a         1524 c         1676 g         1013 t
ORIGIN      
        1 cggtttcttt acgtgggcaa cacggtgtcg aatgacatct ccgcatatgt tgttgatgcg
       61 aatacaggcg ctctgacccc cacgccaggt tcgccgtttc cggccggcct tgtccctctg
      121 gacttgaccg ctggcccgtc cgaccgtttt ctgtatgtcg tcaataacgg gtcgaacgat
      181 ataagcgtgt actctattga tgccgacaca ggtggcctgt cacaggtcca gaccataaca
      241 agcggaggcg ctgccatctc gatcgccatc acggagtagg cgggtgaaga atgagcggta
      301 acctatgaaa aaagaaaaag atttgctctg gacctggctt ttgcccgaga aatcaaggac
      361 ctttgaccgc gctctgtggg tgcgccgcgg cggaaagtgg atcgttttcg ataaagggga
      421 tgcgattgcg gcgctcgcgg aacgattggc gccttacatc gactccggag aaatcgaaag
      481 cgctaaatac tggaacggcg accccagcgc gatcaacgtc tactcactcg atgaggaccg
      541 cggccgtgta aggtcgatcc tgaaggaggc gggcgcccgg catgtgcttg tctgggaata
      601 cgattacgcc atgggcaaga atttcctccg ccctgtcacc ttcgcctatt catggttctc
      661 caagttcagg accattttcg ccagttacgg cctgtcggga tcaatccggc tgctcaaaga
      721 agtgctttcc cagcggcggg gagaaggatg aacggcagat ggaattttga tgtctgtcgc
      781 ttctcccaag gccgccccat ccctgttttt gattgacatt tcagcaccat aaggctaata
      841 ttcgaatctg tctccatcac cgcggaatac gagtaaaaac cggggcttcc acagatgccg
      901 aaagtaaatt ttattttcgc aatccacaac catcagcctg tcggcaattt cgaatttgtg
      961 gcggaggagg cctaccagaa atcctacctg cctttcgtca ggacgcttga gcgccaccgg
     1021 cagatcaaga taacgctcca ttacaccggc atcctctatc gcttcttcga gcagcggcac
     1081 cccgagttca tcgtcctttt gaaggacctt gtcgcggaag gccgggcgga gatcctgtcc
     1141 ggaggatttt acgagccggt catcgcggtg ctgcccgata acgacaagat cggacagata
     1201 cgggcgcttt cggaatacgt gcgaaacaag atcggttacg atgcgcaggg catgtggctc
     1261 gcggagcgcg tctgggaacc gcacctcccg aagcccatcg ccgacgccgg catcaatcac
     1321 gtggtcgtcg acgacttcca cttcaagatg gcggggctgc gcgacgacga actggacggc
     1381 tactacctca cggaggagca ggaccggatc gtccggatct tcccggggag cgagaggctc
     1441 cgctacctga tccccttcca cggccccgaa gaaacgatct cattcctttc cggactccgc
     1501 tcgacggagc gcaaccgcct cgcggtcatg gccgacgacg gtgaaaagtt cggcgtctgg
     1561 cccgaaactt atcgtacggt ttatgaagag ggctggctcg agcgtttttt ttcgctcctg
     1621 gagcagaaca gcgactggct ggagactacg accttcgccg aatacctggg cagggaaccg
     1681 gcgcgcggaa gggtctatct ccccacctcg tcgtacatgg agatgggaga gtgggcgctt
     1741 cctgcccgcg ccatgaagga atacgaagac gcgctggaga aagtgaagaa ttctccggaa
     1801 ttcgcggggc tccggccctt catcaagggc ggcacctgga ggaacttcct cgccaagtat
     1861 tccgagagca accacatgca caagcgcatg cagatgacga gccggaaggt ccacgcggcg
     1921 ctcgacgtcc cgggaacacg aaagaacgcc gatgcggcca ggatgctcga ccatctctac
     1981 caatcgcagt gcaacgacgc atactggcat ggcattttcg gcgggctcta cctgccccac
     2041 ctgcgctcgg gtgtctacga gcagctgatc cgggcggaat atctggccga taaggctttg
     2101 aaaggacgag ggacgaggga cgagggacga ggggaaaagc aaaaaaacca gacagcctcg
     2161 tggctggaca tcgaacaggg cgattttgac ctggacggga acgatgaact tatgctcaac
     2221 tccgagctct tgaacatctt catcgacccg gcagagggag gccggatatc cgaactcgac
     2281 tggaagcccc ggatgttcaa tctcgcgaac acactcacgc gaaggcgtga aggctaccac
     2341 gacaagatca tccgcgccgc cgcggcgaag acggacgcag cgcaggcagg gggcgtaaag
     2401 accattcatg aacgcgtggc cgtcaaggag gaggggctcc agtatcatct tttgtatgac
     2461 tggtataccc gcggatcgct catcgaccat ttcctcgggt caggcgtgga cctggctgcc
     2521 ttcatacgct gcgaatacta cgaagcggga gacttcgtcc tcgggaacta cggcgccgct
     2581 cgaaaaaaag cggggggttc cgcggccgtc atccttgagc gggtggggaa cgtggcgggg
     2641 ctcgccgtcc gcctccggaa ggagatatcc ctgcgccgga gcgcgcccga attcccggtg
     2701 cggtacgaga tcgaaaacct gggcgccgaa gagctgaata cctcgttcgg cagcgagttt
     2761 aatttttcgt ttctcgccgg aagcgcccac gaccgatact atgacatccc gggccacacg
     2821 ctggacagga ggaacctcgc ttcctccggc gaaacgaaca acgtgcgcga ggtcagtctc
     2881 gtcgatgaat ggctcaagct caggctcacg ctgtcatttg acgagccggc catgctctgg
     2941 cgcgctcccg tagagacggt gtcccagtcc gaggccgggt tcgaacgggt ctaccagagt
     3001 tccctgatca tgccggtctg gcgcatttcg ctcggtcccg ggaaatcctg gaatgcggag
     3061 atacgggtcc gggtggaata acggcaagaa tattttgcca cagagatcac ggggaacaca
     3121 ggtaacaaaa aagagaatag aatttttctg gccggattca aaagcttttc tctgtgcgct
     3181 ctgcggttaa atagcttttc ccgggttggg gaattgatac tttgaagatc gaaagaaaag
     3241 gggtcgtctc gttcatgaaa aaattgattc ttctgtttct gctgattgcc gtcgccttcg
     3301 ggaccggtgc ggccgcatcc gaaacacccc tcggggtgga ggagatcgag tccgtcgacg
     3361 agctggcaac cgggattctg tcgtactttc cccgggtcca aggcgaggtc aaggccgtca
     3421 atggagagcg gatcaccgtt acgctcggga ccaaggacgg gatcgcgcca ggcgtcgtgc
     3481 tcaccctgtg gagggacagc aaggagatcc tgcatcccgt gaccggcaag gtgatcggac
     3541 ggacggagga tgaagtcggc gacgctcagg taacggacgt gatggaaacc gcgtgcacgg
     3601 cggtgatgag gaaacgcctg aaggacccga aggagggcga taaggcgagg atcacgccga
     3661 agaaaatcag catggcgctg ctgccgctgc gggccgaccg gcccgagacg cttcagggcc
     3721 tcgccgagcg gctgagcgag tccgggaggt tctcgctctt ggacagcggg acggtcgccg
     3781 ggttcctcag ggaccgcaag gaacgcgacc gttcgttgct caaggagatg ggcaaggcgt
     3841 acaacctcga tgtcatcgcg gctgtcggga tctatcccac cgaagcaaac aagctgctcg
     3901 tcacggtcag gctcttctac gcggacgagg ccaggccgat cgacacgatc gtcgcgatgc
     3961 tggacctgaa gtcccgaaag gaagccatcg ccgaagtgaa gccgttcttc gccccggtga
     4021 cggcggagaa gaatgacgta acggcgctga cctttgacgc gcagctcatc gccgcggccg
     4081 atttggaagg aagcgggtct ctgcaatatg cgttttccga cggcgtcaaa cttcatatct
     4141 accggcaggg agccgcggga tggcgcgagg attgggtcga gcacgtgccc gccggcgccg
     4201 gggaaattca gaccatcaac ctcgatgccg ccgacatcaa cggcaacggc aggtccgagc
     4261 tcttcgtaac catcatgcag agcgggaagg tcgtctcccg cgtctttgaa ttccaggaag
     4321 gcgcttatca tcagaccgcc gaaatccccg gctttctccg cgtcattacc tatccgggca
     4381 tggggaccat gctgatcggc agggcgtatg accctgtcaa gtttttcagc ggtccggtcc
     4441 gccagtacca gtggtcgaac ggcgcataca ctgcaggtgc ggaaatggcc ctgcccgagg
     4501 acgtgggtct ctacgggttc gtggtggcgt cgctcggaga gaaggcgccg ctgctcgtcg
     4561 cccttaacga cgaggaccgg ctgcgcgtct attccggcga gaccccgatc tggaagagcg
     4621 aggagtccta cccgtccgtc gggacatcgg tgatgaaacc gacaaccggt atcggggcgg
     4681 tcctttccca ggagacggaa gttgtggaca agtcgcggaa agtccggatt ccggggaggg
     4741 tcattgccct cgacctgaac ggggacggca gggatgagat cctcctcccg aagaacagcg
     4801 gcgggacgat tctgagggga catacgcagg cagaattcgt cagcctcggc tggacaggag
     4861 cgcggctcga gcagaaatgg gccatcaaag acgtgggggg cgccgtagtc gatttccagg
     4921 tcctgcggca ggaagccacc ggcgcccgaa tcctggcgct ggtgaggtcc tctggcgggt
     4981 tgttctcccg cgacacgatc aaggttgtga gctatccggc gaagtgaatc aaatgcggat
     5041 ggccgatgaa acgggccacg ctgatgcgga tttcggaatg cggaatagaa ctatgaaccg
     5101 ggactatgaa taaaaaaagc ccttcccgcg aagaacagct cgacaaggcg ggccttccgc
     5161 gccacgtcgc cattatcatg gacggaaacg gccggtgggc catgcgcaag ggcctgccgc
     5221 gcattgccgg tcataagcgg ggcgttgaca ccgttcgccg cgtcacggaa gagtgccgga
     5281 ggatcggcat cgaagtcctg acgctgtatg ccttttccga cgagaactgg ggaaggccca
     5341 aggaagaggt cgggtttctc atggacctgc tggggacctt tct
//
LOCUS       DASSLD010000606         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_310933,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000606 DASSLD010000000
VERSION     DASSLD010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310933"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFK23_10920"
     CDS             <1..645
                     /locus_tag="VFK23_10920"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7319638.1"
                     /translation="REKVLSYIGWVLISIWSRTIRIQFIEKKIPDQLRAEGNNLIYAF
                     WHGRQFLLFHNHRNTGIVIPASESRDGDIQAGVLLHFGFDVVRGSSKRKGDRALLGLV
                     DGLRKGKDIALAVDGPRGPIYEVKQGITYLAGKLNKPIVPVATSAKRFWILEKIWDKY
                     MLPVPFTRGVIVYGEPIVVRGIQEEELESKRRELTDALNRVMSRADAYFHRQAS"
     gene            657..1919
                     /locus_tag="VFK23_10925"
     CDS             657..1919
                     /locus_tag="VFK23_10925"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HET7319639.1"
                     /translation="MYILYNMLLVLLTVLLSPVILFKLITVPKYRGGISQKLGRIRKR
                     VRKAIQGTRPIWVHAVSVGEVMAAHPLIRELKKKYPNRKLILSTVTVTGNHTAQQRVP
                     EADAVFFFPFDFPWIVRRVIREINPAIVLVAETELWPNFFRELKRADIPSAVINGRIS
                     PHSFRNYMKFKKFFSQVFSNVTIFCMQSADDAARIKEIGAPSDRVMVTGNLKFDQKIP
                     ANQPSPVQLPKGSKVITAGSTHRGEEAALLDVFRRLREKFPELILIIAPRHPERFNEV
                     EGLINKAGYECQRRTRSKGTIKDVLLLDTIGELRSFYGICDLAFVGGSLVKVGGHNLL
                     EPAAMKKPVIFSRYMYNFKEISEALISAGGGILVKDKDELHVQAEKLLSDKNYAWQIG
                     SKAFAVIEANSGATKRTIDAVCNLIREN"
BASE COUNT          531 a          474 c          534 g          474 t
ORIGIN      
        1 agggagaaag ttttatcata catcggttgg gtgctcatct cgatttggag caggacgata
       61 cgtattcagt ttatagagaa gaaaattccc gaccagctaa gagcagaagg caataatctc
      121 atctatgcat tctggcatgg aaggcagttc ctgctgttcc ataaccatcg caataccggg
      181 attgttattc cagccagtga aagccgagac ggagatattc aggccggggt tttactgcat
      241 tttggatttg acgtagtgcg tgggtcgagc aaacggaagg gcgatcgggc gctcctcgga
      301 ctggttgatg gactgcgaaa aggcaaggac attgctctcg ccgtagatgg tccacgcggc
      361 cctatttacg aggtgaaaca ggggataact tatcttgcag gcaaactgaa caaacccatt
      421 gttcccgtcg caacaagcgc gaaacggttc tggattctcg aaaagatatg ggacaaatac
      481 atgctccccg tgccatttac acgaggtgtg atcgtgtacg gagagcccat cgttgtccgg
      541 gggatacaag aggaagagct tgagtccaag cgaagggaac tgacggatgc cctgaaccgc
      601 gtgatgagcc gggctgatgc gtactttcac cggcaagctt cgtagagggt gaggacatgt
      661 acattcttta caacatgctg ttggttctgc tgacagttct cctgtccccg gtcattctat
      721 tcaagctcat aactgtgccg aaataccgcg gcggcatatc gcaaaagtta ggccgcatac
      781 gaaagcgggt ccgaaaggca atccagggaa ctcggcccat ctgggtgcat gccgtatcgg
      841 tgggcgaagt catggcagcg caccccctaa tccgcgaact gaagaaaaaa taccccaaca
      901 ggaagctgat tctctcgacg gtcacggtca ccggcaacca tactgctcag cagagggttc
      961 cggaggccga tgcagtgttc tttttcccct ttgatttccc ctggatcgtg cgcagagtaa
     1021 tacgcgaaat caaccctgct atcgtgctcg tggcggaaac ggagctctgg cccaattttt
     1081 tccgcgaact gaagagggcg gacattccat cggctgtaat caacggaagg atatctccgc
     1141 actcgttcag gaattacatg aaatttaaga agttctttag ccaggtcttt tcgaatgtaa
     1201 ccatcttttg tatgcagtct gccgatgatg cggcgcggat aaaggaaatt ggtgctccct
     1261 cggatagagt aatggtcacg ggaaacctca aattcgacca gaagatcccc gcgaaccagc
     1321 caagccctgt ccagttacca aagggcagta aggtaataac cgcgggaagc acacaccggg
     1381 gggaggaggc cgcactgctt gacgtattca ggagattgcg ggaaaagttc cccgagttga
     1441 tccttattat tgccccgcgt catccggaga ggttcaatga agttgaaggg ctgatcaaca
     1501 aggcagggta tgaatgtcag cggagaacga ggtcaaaagg gacaatcaaa gacgttcttc
     1561 ttcttgatac catcggtgaa ttgcgttcat tttatggtat ctgtgacctt gcttttgtcg
     1621 gtggcagcct cgtaaaggtc ggaggccata atctcctaga acctgcggca atgaaaaaac
     1681 ctgttatttt cagccgttac atgtataact ttaaagaaat atctgaggct cttatctctg
     1741 ctggtggcgg catactcgta aaagataagg atgagcttca tgttcaggcc gaaaagctgt
     1801 tgtcagacaa aaactatgct tggcaaatcg gaagcaaggc gttcgctgta atcgaggcaa
     1861 actccggggc aacgaaaagg acgattgacg ctgtctgcaa tctcatacgt gaaaactaaa
     1921 gcgcattctt atatcaaaga ccttatatac ggaagaaaaa aatcacttcc cgtcggggcc
     1981 ttcctgcggc tactctcttt gttttacgct ctc
//
LOCUS       DASSLD010000607         4041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_746304,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000607 DASSLD010000000
VERSION     DASSLD010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746304"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VFK23_10930"
     CDS             complement(<1..775)
                     /locus_tag="VFK23_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HET7319640.1"
                     /translation="MEKTAKEMEESLSRLSPWWRNAAILIVVMGLSVLVWIAARSYQD
                     APPIPAKVVGPAGEKIFTRDDIVAGQQVFLKYGLMENGTIWGHGAYLGPDFSAAYLHT
                     LSQEMGETIARTRFNRVLGDLVAAEHDAVDAETGQWLKQNRYDPHTETLAFTQPEVAS
                     YRKQIADWADYFSKPTGNGGLPAKYIKDPNELRQLAAFFAWTAWASVANRPGKPYSYT
                     NNFPYDPVAGNTPTGDAVLWSALSLITLLVGTAAVLFAFG"
     gene            1089..1490
                     /locus_tag="VFK23_10935"
     CDS             1089..1490
                     /locus_tag="VFK23_10935"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HET7319641.1"
                     /translation="MTKRKNAFRQPDGFNSLPLEHRLLFEFTEPGLAARISGACFGQR
                     FVGQGAVTFFWSVIPYRMEWRYGPAAHRVLPMDVGHVCQNLYLSAEAIGAGVCAVAAY
                     DQEVLDALLNLDGEEEFVIYLAPVGKHAISA"
     gene            complement(1517..2110)
                     /locus_tag="VFK23_10940"
     CDS             complement(1517..2110)
                     /locus_tag="VFK23_10940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7319642.1"
                     /translation="MWHERIGSLLPNRWKFQWRYWRRQTPWDTQVTPPEVMEFIARTP
                     PGKALDLGCGTGTNAITLARYGWRVTGVDFIPKAILAARAKAARGGFAVDFLVASVTD
                     LSALSGPFDYALDIGCLHALKAEDRIRYAVNLSRLLRPQAWYMLYAWLPRPWEGGVVG
                     ISTEGVEILLREDFSKVRTEIGEENGNLSSWYWFQRR"
     gene            2375..2926
                     /locus_tag="VFK23_10945"
     CDS             2375..2926
                     /locus_tag="VFK23_10945"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7319643.1"
                     /translation="MRQRAKVVPFALGSVLEVGFGSGLNLPFYDKTKVQHVWALDPSR
                     EMWALANKRVRGSDLPIEFLNATAEEIPLPNRSVDTVLVTFTLCTLPDVQRALREMAR
                     VLKPGGELVFCEHGLAPDENVRRWQNRLNPIWKFFGGGCNLNRHIPSLLEQGGFRLRE
                     MSSMYIPGWRPASFNYWGRALGS"
     gene            complement(3337..3777)
                     /locus_tag="VFK23_10950"
     CDS             complement(3337..3777)
                     /locus_tag="VFK23_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319644.1"
                     /translation="MISRKLVKMIEEHAEQITQGLLKDIQTNPKTSSYQQFSVEEMYV
                     RAYTVYKNLGEWLVDKKGAEAQKRYLDLGRRRFEEKIPVSHVIFALVLTKNHLLKYIK
                     MFGLVDTAIELHRELELFSLVTEFFDNAIYYTVRGYEEMSGVKQ"
BASE COUNT          886 a         1138 c         1047 g          970 t
ORIGIN      
        1 tgccaaaagc gaacaggacc gccgccgtcc ctacgagaag cgtgatcagg ctgagggcgc
       61 tccagaggac ggcgtcgcca gtcggcgtgt tgccggccac cgggtcgtag gggaagttgt
      121 tcgtgtacga gtacggcttg cccggacgat tggcgacgga ggcccaggcg gtccaggcaa
      181 aaaaggctgc cagttgacgc agttcattcg gatccttgat gtacttggca ggaagcccgc
      241 cgtttccagt cggtttggag aaatagtcag cccaatcggc tatctgctta cggtaggagg
      301 caacttcggg ctgggtaaag gccagtgtct ccgtgtgcgg atcgtaacgg ttctgcttga
      361 gccattgccc cgtttccgca tcaacagcgt catgttctgc ggcgacgagg tcacctagaa
      421 cacggttgaa acgcgtacgg gcgatcgtct ctcccatctc ttggctgagc gtatggaggt
      481 aagcggcgga gaaatcagga ccaaggtagg caccgtgccc ccagatggtc ccgttttcca
      541 tcaggccata cttgaggaac acctgttgcc cagcaacgat gtcgtccctg gtaaagatct
      601 tttcccctgc cgggccgaca acctttgccg ggatcggtgg ggcatcctga taacttctgg
      661 cagcgatcca aaccagcacc gataatccca taaccacgat gaggattgcg gcgttccgcc
      721 accagggcga aaggcgcgag agcgattcct ccatttcttt tgccgttttt tccatttgcc
      781 acccctttcg ccgttgatgt ggaggtcgtc tttgcggcag cactaattgc cataacggct
      841 ttcttgtgcc gacccttcag tcccactgtt catcgacagg atcattcccc tcaaagggct
      901 cgatcctcgt ggcaacattg cctcctcgac ataggaaaac ggtggggcaa cttcaggtac
      961 cgatcctccg gaaatgtttt atccttccca agccgctatt ggaacaattg gtcggaggga
     1021 accgtcgtaa cgctctcata aaaagcactt ccaccaaata ccgccttacg ttgcgccggg
     1081 ttaagaggat gaccaagaga aaaaatgcct tccggcagcc agatggcttc aattccttgc
     1141 cgctggagca ccgccttctg ttcgagttca cggaaccggg cctggccgct cgcatctccg
     1201 gggcctgttt cggtcagcgg ttcgtcgggc agggggccgt cacgttcttc tggagcgtca
     1261 tcccgtacag gatggagtgg cggtacggac ctgccgcgca ccgcgtcctc ccgatggatg
     1321 tcggccacgt ctgccagaac ctgtaccttt ccgcggaggc gatcggggcg ggggtgtgcg
     1381 cagttgcagc gtacgatcaa gaggtcctcg atgcccttct aaacctcgac ggggaggagg
     1441 agttcgtcat ctacctggca ccggtcggca aacacgctat atccgcgtaa aaggaaaaca
     1501 cggaaattcg tcccccttaa cgccgctgaa accagtacca cgaggagagg ttgccgttct
     1561 cttctccgat ctccgtccgt actttggaga aatcctctcg cagcaagatt tcaacccctt
     1621 cggtggaaat gccgacaacc ccgccttccc atggcctcgg cagccaggcg tagagcatgt
     1681 accaggcctg cggccgcagc aatcgggaca ggttcacggc atatcgtatc cgatcttccg
     1741 ctttcagggc atgcaggcag ccgatgtcca gagcgtagtc gaaaggaccg gacagggcgg
     1801 aaaggtcggt gacgcttgca acaagaaaat cgaccgcaaa tccgccccgg gccgccttgg
     1861 cgcgagcggc aaggatggct ttgggtatga aatcgacgcc cgtgacccgc catccgtacc
     1921 gggccagggt gatggcattg gtgcccgtgc cgcatcccag atccagcgct tttccgggcg
     1981 gggtccgggc gatgaattcc atcacctcgg gcggggtgac ttgagtgtcc caaggtgtct
     2041 gccggcgcca atagcgccac tggaatttcc atcggttggg cagcagggat ccgatccgtt
     2101 catgccacat atccggaaac cccttcccct ttttcaaaag tcgagcaccc atctgcgcgc
     2161 agccacggtc ccaccactcc tctgatcgct gattcattta ttcgtactaa cggaagttct
     2221 atgcaaaaat agtaaagggt cgccacgttt ttacgatcgg ttatgcgggc atcttgagtc
     2281 catttgattt ggatcagggc cgagtgtgat gagtctatac acgcgcttca tactcccgaa
     2341 aattgtagac ttcacctgca acttgaagcc caacatgcga cagcgggcaa aagtggttcc
     2401 cttcgccctg gggagcgttc tggaggttgg tttcggttcg gggctgaacc tgcccttcta
     2461 cgacaagacc aaggtacaac atgtctgggc actcgatcca tccagagaaa tgtgggcctt
     2521 ggccaataag agggtgcgtg gttccgacct acctatcgag ttcctgaatg ctacggccga
     2581 agagatcccc ttgcctaacc ggagtgtgga caccgtgctc gtgacattta cgctttgtac
     2641 ccttcccgat gtccagcggg cactccgtga aatggctcgg gttctcaaac cgggtggaga
     2701 actcgttttt tgcgaacacg gcctggcacc tgatgagaat gtccggcgat ggcagaatcg
     2761 cttgaatccg atctggaagt ttttcggtgg tggctgcaat ctcaatcgac acatcccctc
     2821 tctcctggag cagggaggct ttcggttgcg cgagatgtcc tcgatgtata taccggggtg
     2881 gagacccgcg agctttaact attggggacg cgctctaggg tcttaatgtc tcccatcgta
     2941 agggcaggtt atatgaaaga ttggcaagtc cctcttcttt tcgttggaac cacaaattcg
     3001 ccactcgggc cgaataaacg aaaaaaggga acccggtgtt aatccggatt ccttttcttg
     3061 attccgatgg tgccgaaggg gggaactcga acacccacgc gtttccccac cactccctca
     3121 agatgaggag ggttgggtcg caatgtgttc cactcctcgt tgcccgaatg aacagaaaga
     3181 atcctcccca acgtcctctg acggaaatga cggaaatccg gtcatcaatt ttaattaagc
     3241 tgagatggag caaacatcac ccgatatcct tataggcata ggcaaaatcc gtgtcgaaag
     3301 aatgattacg ttgtcttgtc ctctctcgcc cttaccttat tgctttactc cactcatttc
     3361 ttcatatccc ctaacggtat aatagatcgc attgtcaaaa aattccgtca ccaaactaaa
     3421 taactccagc tcccggtgaa gctcgatcgc tgtgtccact aggccaaaca tttttatata
     3481 tttcaatagg tgatttttcg ttaaaactag ggcaaaaatg acatgactca ccggaatttt
     3541 ttcctcaaac cgccgtctcc ccaggtccag ataccttttt tgggcctccg cccctttttt
     3601 gtctaccagc cactcgccca gatttttata aacggtgtaa gccctaacgt acatctcttc
     3661 gaccgaaaac tgttgatagg aactggtttt tgggtttgtt tgaatgtctt taagaagtcc
     3721 ctgggttatc tgttcggcgt gctcttcaat cattttcaca agctttctcg agatcataag
     3781 cttccctcta ttccttttaa ctgacaacga tattgttttt tattcatatt atcacgtaac
     3841 tccaagtcct tatccatcat gattcgctta ttacatgccg tcactgcatg agtgtagtca
     3901 actcccacga aaatgcgtgc cgctgcgcgg gggtcccccc ccttcaaagt ggtccactgc
     3961 ttatcattat caacagaaac ttaatcaatt gagaagtttc gcaccaacgt cttcttgatg
     4021 gaaggacgga aatcccgtca t
//
LOCUS       DASSLD010000608         2157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_581073,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000608 DASSLD010000000
VERSION     DASSLD010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581073"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFK23_10955"
     CDS             complement(<1..627)
                     /locus_tag="VFK23_10955"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57"
                     /protein_id="HET7319645.1"
                     /translation="MSDYPLSIAFVWHMHQPYYKDDITGSYILPWVRMHGIKDYYDMP
                     ALLEDFPRIHQTFNLVPSLLKQIRDYVDNGATDRFLALTLKPAQELDEDDKLFLLRSF
                     FMASWDTMIRPYPSYWQLLDRRGYSVSPNDLLNATRYYTTQDYLDLQVWFNLTWFDPL
                     FKNNDPFLKGLIQKGSGFTENDKALLVQKQREVMALIIPEYRKLSEMGR"
     gene            complement(789..1055)
                     /locus_tag="VFK23_10960"
     CDS             complement(789..1055)
                     /locus_tag="VFK23_10960"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET7319646.1"
                     /translation="MLFAAVFLAAAYLLASLIFGEMGLIKYFRMKARYDSLTREIAEL
                     RHDNARLMRDVRVLRSDPDHLERIARDKLGLARPGEIVYYYGEP"
     gene            1305..1565
                     /locus_tag="VFK23_10965"
     CDS             1305..1565
                     /locus_tag="VFK23_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319647.1"
                     /translation="MPARKTSQKQSHEDRLRGSVSAIQLFQDSHIHQRYVAYFFFVVF
                     FAFFAGAFFVAITHHPLPFQGLRNEMGKMMPLHTAPLRLPVI"
     gene            complement(1734..>2157)
                     /locus_tag="VFK23_10970"
     CDS             complement(1734..>2157)
                     /locus_tag="VFK23_10970"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HET7319648.1"
                     /translation="IIALITRADRDLSTSRILQDKDEEWAFAAAYLAMVRSARALILS
                     EGFRPKGVRGRDAQKTVVTAAGVLLGEQYKSLINKFDRMRRKYQSFMEEAGRIISRYE
                     AGQAIKDAEEFQGLVNGKIREKYSQMSLLNDVPQLVSR"
BASE COUNT          470 a          584 c          558 g          545 t
ORIGIN      
        1 cctgcccatc tctgaaagct tccggtactc ggggatgata agcgccatca cctcgcgctg
       61 tttctggacc aggagcgcct tatcgttctc ggtaaatccc gatcccttct ggatgagccc
      121 cttgagaaac ggatcgttat tcttaaagag cggatcgaac caggtcaggt tgaaccagac
      181 ctgaaggtcg agatagtcct gcgtcgtgta ataccgggtt gcgtttaaaa ggtcgttggg
      241 agaaacgctg tagcccctcc ggtcgaggag ctgccagtag gatggatagg gcctgatcat
      301 ggtgtcccag ctcgccatga agaagctcct gagcaggaac agcttgtcgt cctcgtcgag
      361 ttcttgggca ggcttgagcg tcagggcgag aaacctgtcg gtcgccccgt tgtcgacgta
      421 gtcgcggatc tgcttgagga gagaaggaac gagattgaag gtctggtgga tccgggggaa
      481 atcctcgaga agcgccggca tgtcgtaata atccttgatg ccgtgcatcc gcacccacgg
      541 gaggatatag gaacccgtga tatcgtcctt gtagtaaggc tggtgcatgt gccagacgaa
      601 ggctatggag agcgggtagt cggacatggc ttacgggaag cgccaatcat aaacaaatta
      661 caaattacta atcgcaaatt tcaaataagt tccaaaaatt aaagatcaat ttcagaaacg
      721 cccgagcctt ggtgcttgat gcttggaatt tgtctgcaat ttggattttg atatttggaa
      781 tttatcgatt acggttcgcc gtaatagtac actatctcgc ccgggcgcgc aaggccgagt
      841 ttgtcacggg cgatcctctc caagtggtcg ggatcgctcc taaggacgcg gacgtccctc
      901 atcagcctgg cattgtcatg acggagctct gcgatttccc gggtgaggga atcgtaccgg
      961 gccttcattc ggaaatattt gatcaacccc atctcgccga agatcaggga cgcaaggagg
     1021 taggcggccg caagaaagac cgcggcgaaa agcattttcc ggttgcggac attccgggag
     1081 ttccggtcgg attttaaata attccgtttg cgcatggctc gacgtattat gccacgatct
     1141 tctaatatta caagtatcaa agcagcatag cattacacgt atcgttcccg catggctttt
     1201 aagcgggaat ccattttaca acgtcgagat ggaattcccg gttttaatca tgccggcagt
     1261 ctcgcggaga acgcttaccg caatgacggc tttcgcaatt ttacgtgcct gcacgaaaga
     1321 cctctcaaaa acaaagccac gaagaccggc ttcgtggcag tgtgtcagca attcagcttt
     1381 ttcaggattc tcatatccat caaagatacg tcgcttattt cttttttgtg gtcttcttcg
     1441 ccttttttgc cggggctttc ttcgtcgcca ttactcatca cccccttcct ttccaaggat
     1501 taaggaatga aatgggaaaa atgatgccgc tgcacactgc tccgcttcga ttgccggtca
     1561 tttaatacgc cgggggcatt cctgcccccg gcgggcccca ctacgggaca cggtgtataa
     1621 aaactacttc tttttggtgg ccttcttctt ggtcgccttc ttcttggcct tcttcttggc
     1681 tgccattagc tctccacccc ctttcggcat accgcattcg gacccaagcg gccttatctt
     1741 gaaacaagct gaggtacatc gttcagcagt gacatctgcg agtacttctc ccggatcttg
     1801 ccgttcacga gcccctggaa ttcttcggcg tccttgatgg cctgcccggc ctcatagcgc
     1861 gatatgatgc ggccggcctc ctccatgaaa ctctgatatt ttctcctcat ccggtcaaac
     1921 ttattgataa ggcttttata ctgctcgccg agaagcaccc ctgccgccgt caccaccgtc
     1981 ttctgggcgt ctctgccccg tacccctttc ggccggaacc cctcggaaag gatcagggcc
     2041 ctcgccgagc gaaccatggc aaggtaggcg gcggcaaacg cccattcttc atctttgtcc
     2101 tgcaggatcc tgctcgtaga gaggtcccga tccgccctcg tgataagggc gatgatt
//
LOCUS       DASSLD010000609         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_763253,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000609 DASSLD010000000
VERSION     DASSLD010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763253"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VFK23_10975"
     CDS             complement(<1..535)
                     /locus_tag="VFK23_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HET7319649.1"
                     /translation="MAMSGRLGSMLVSSGLITDDQLKKALSVQKTEGGRLGSILVKLG
                     FVQEDKLMTFLSKQYGVPYVDLSKFEINPAVIKHIPAEVAQKYRIMPINRAGATITIA
                     MVDPSNIFAIDDIKFMTGYNVEAVVATEGAIVEAVKRYYGGAKNLVAAKPAGGAASAA
                     QKEEKKLSIEAKDYALDD"
     gene            complement(630..1496)
                     /locus_tag="VFK23_10980"
     CDS             complement(630..1496)
                     /locus_tag="VFK23_10980"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET7319650.1"
                     /translation="MESVISGKTKIYGIFGHPVEHTFSPGMHNAAFRKLGMDACYVPF
                     SVHPSRLRDAVKAVLSLGLCGLNITVPHKEKVIARLDELSEEARLIGAVNTVEVREGR
                     LVGHNTDGRGFIRSLREGAGFDPRGKNVLIAGAGGAARAVGFCLALAKAERIVFHDVD
                     AGKAARLARDIRKKTGVISDAISSHGLSAAARDAHCLINATPLGLKKGDPLPLGRELL
                     LPHHLVYDLVYNPPETGLLRAAKDRGARSLSGIGMLLFQGVIAFEIWTGAQAPVAVMK
                     NALTRQLKGRAR"
     gene            complement(1503..>2367)
                     /locus_tag="VFK23_10985"
     CDS             complement(1503..>2367)
                     /locus_tag="VFK23_10985"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HET7319651.1"
                     /translation="SPLYISVHATDPALRSFILGNSRAPDIMMSMRRLAAGGISMHAQ
                     IVLCPGVNDGPFLLRTLNDLSGLFPSVLSIAVVPVGLTAFRKGLFPLRAFSRKEARAV
                     VDEVTRFGRSWKKRHGTRLVFASDEFYVKAGIPVPPASFYEDFPQIENGVGMVSDFLR
                     GARRTRIPASVSAFRATLVTGLSFRRMLRDAAARLGGVRGLSLRILAVRNKFFGESVT
                     VAGLLTGSDIAAALEGKPRGDLVVIPAEALNDDGIFLDGMSLEQLRGRFSVPIAPAKS
                     FQELVMLLKRL"
BASE COUNT          472 a          711 c          701 g          483 t
ORIGIN      
        1 cgtcgtccag ggcatagtcc ttggcttcga tgctcagctt cttttcttct ttttgagcgg
       61 cagacgcagc gccccccgcc ggtttggcgg caacgaggtt tttcgccccg ccgtaatatc
      121 ttttaaccgc ttcgacgatc gccccttcgg tcgcaaccac cgcttcgacg ttgtaaccgg
      181 tcatgaactt gatgtcatcg atggcgaaga tgttcgaggg gtccaccata gctatggtga
      241 tggtggcgcc ggcccggttg atcggcatga ttcggtattt ctgggccact tcagcgggta
      301 tatgctttat gacggcgggg ttgatctcaa atttgctgag gtcgacgtag ggcactccgt
      361 actgcttgct caagaaggtc attaacttgt cttcctggac aaaccccagt ttgaccagaa
      421 tggaacccag ccggccgccc tccgtcttct gcaccgagag cgccttcttg agctgatcat
      481 cggttatcag gcccgacgac acgagcatag agccgagcct tccggacatc gccatagaaa
      541 ctccttcaaa actccttcaa ctgaaaaaaa tcagtgatta tattacttta atgaccgtgg
      601 gatgtcaaga tgtgtctgct gattcccgtt catcgtgcgc ggcctttgag ctggcgggtc
      661 aacgcgttct tcatgaccgc gacgggggcc tgagccccgg tccagatttc gaacgcgatg
      721 actccctgga acagaagcat gccgatgccg gaaaggcttc gcgcgccccg gtctttcgcc
      781 gccctgagca gtccggtctc gggtggatta tagacaagat cgtataccag gtgatgcggc
      841 aggagcagtt ccctgccgag cgggagcgga tcgccctttt tgagtccgag cggcgtggcg
      901 ttaatcaggc agtgggcatc tcttgcggcg gcggaaaggc catgagaaga tatcgcatcg
      961 gaaatgacgc cggtcttttt ccggatatca cgtgcgagcc ttgcggcttt cccggcgtcg
     1021 acgtcgtgga aaacaatcct ttccgccttc gcgagcgcga ggcagaagcc cacggcccgc
     1081 gctgctccgc ccgcgcctgc gatcagaacg ttcttacccc ggggatcgaa tccggcgcct
     1141 tctcgcagcg agcggatgaa tccccggccg tccgtattat gaccgacgag gcgtccttcc
     1201 cgcacttcca cggtgttcac cgcgccgatc agccgggcct cctccgaaag ctcgtccagc
     1261 cgggcgatga ccttctcctt gtggggcacc gtgatgttca ggccgcagag ccccagggaa
     1321 agcacggctt ttaccgcgtc cctgagccgc gagggatgaa cgctgaaggg cacataacag
     1381 gcatccatgc cgagcttcct gaatgcggcg ttatgcatgc cgggcgaaaa ggtatgctca
     1441 acagggtgcc cgaaaatccc gtagattttt gtttttcctg atatcacgct ttccattcct
     1501 ccttacagcc gtttaagcag cataaccaat tcctgaaagc tcttcgcagg cgctatcggt
     1561 acgctgaaac ggccgcgaag ctgttcgagg ctcattccgt cgaggaagat gccgtcgtca
     1621 ttgagggctt cagcgggaat gacgacgagg tccccgcgtg gcttgccctc cagggccgct
     1681 gcaatatcgc tccccgtcaa cagaccggct acggtaaccg attcgccgaa gaatttattt
     1741 ctgacggcga gaatgcgcag cgacaggccc cggacaccgc ccagccgcgc agcggcatcg
     1801 cgcagcattc ttctgaagga aaggccggta acgagcgtcg cgcggaaggc ggatacggaa
     1861 gccggtatgc gggtgcgccg agcaccgcgt agaaagtcgg agaccatgcc gacgccgttc
     1921 tctatctggg ggaagtcctc atagaaggaa gcgggcggca cgggaattcc cgccttcacg
     1981 tagaattcgt cggaggcaaa tacgagacgc gttccgtgcc tttttttcca cgacctcccg
     2041 aaccgggtca cctcgtcgac gacggcgcgg gcctccttcc gcgaaaacgc cctgagcgga
     2101 aacagcccct tccggaaggc ggtcagcccg accggaacga cggcgatgga gagaacggag
     2161 ggaaacaggc ccgacagatc gttcaaggtc ctaagtagaa acggcccgtc gttgacgccc
     2221 gggcagagca cgatctgcgc atgcatgctg atgccgcccg cggccagcct cctcatgctc
     2281 atcatgatgt ccggagccct gctgttgccc aggataaagg accggagcgc ggggtcggtg
     2341 gcatggacgg aaatgtacag gggggag
//
LOCUS       DASSLD010000610         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_258677,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000610 DASSLD010000000
VERSION     DASSLD010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258677"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="VFK23_10990"
     CDS             complement(<1..1131)
                     /locus_tag="VFK23_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HET7319652.1"
                     /translation="MAYESLRDFIAVLEKNGELVRIKAEVDAELEIAEITDRVSKQKG
                     TENKALFFERVKDSRFPVLTNAFGSMKRMCMSLEVADLDDIGKRIKDIVDPTNLFPGP
                     GAGFMDKLQTLPKLAELANFFPKTVKKAPCQEIVLTGEQVDLSKIPVLKCWPADGGRF
                     ITLPMVCTVDPATKITNVGMYRMQVFDHQTTGMHWHKHKDGARHYQHYEAAGKRMEVA
                     VAIGGDPAIIYSSTAPLPPAIGEFVFAGFLRKKPLEVVQCKTVDVRVPAEAEFVLEGF
                     VDPGERRREGPFGDHTGYYSEADQYPVFHITAITHRKDALYPATLVGRPPQEDAYLGK
                     ATERIFLPLLQMVAPDILDMDMPVEGVFHNNVIVKIRKRYAGH"
     gene            complement(1205..1612)
                     /locus_tag="VFK23_10995"
     CDS             complement(1205..1612)
                     /locus_tag="VFK23_10995"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HET7319653.1"
                     /translation="MQVTFKKRERGGIEFGIIYGGIAVAALAVPWFLPVRLLSPSCAF
                     KGLFDIPCPTCGATRAVVHLSHGEIAFALAMNPLISIAMVFAVLFFFYSLITLLFDSR
                     RIGFILTEREKNAVRSVAIVLLLIQWGWLIRTL"
     gene            complement(1705..>2307)
                     /locus_tag="VFK23_11000"
     CDS             complement(1705..>2307)
                     /locus_tag="VFK23_11000"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HET7319654.1"
                     /translation="LLKTLKEVLFTPTDFFRKMPVTGGLTDPLLFAMIVGMTGLLFSY
                     LWDILFYDSLQHFVAPEFRAWSERRMMYDRGFSYFSVLTPFILIIGLFLTSGFLHLML
                     LMVRGAKAGFEATFRVVGYSTSPFVVMIIPFCGMPVTWLWVITLSIIGLREAHETSGG
                     KAAVAVLFPFMLCCGIITMTITLFMGAVAASFGGMMHWYP"
BASE COUNT          522 a          653 c          682 g          450 t
ORIGIN      
        1 gtgtccggcg tagcgcttcc ggatcttcac gatcacgttg ttgtggaaca ccccctcgac
       61 gggcatgtcc atgtccagga tgtcgggagc aaccatctgc aggagcggca ggaatatccg
      121 ctccgtcgcc ttgccgaggt aggcgtcttc ctgaggcggg cgccccacaa gagtggcggg
      181 gtagagggcg tctttcctgt gggtgatggc cgtgatgtgg aatacggggt actggtccgc
      241 ctcggagtag tagcccgtat ggtccccgaa cgggccttcc ctccttcgct cgccgggatc
      301 gacgaacccc tcgagcacga actctgcctc cgccggaacc cggacgtcca cggtcttgca
      361 ctggacgacc tcgagcggct tcttccgcaa gaacccggcg aagacgaact ctccgatggc
      421 cggaggcagc ggcgcggtgg aggaatagat gattgcaggg tctccgccga tggcgacggc
      481 cacctccatg cgtttccccg cggcctcata atgctgataa tggcgcgcgc catccttatg
      541 cttgtgccag tgcatgcccg tagtctggtg gtcgaagacc tgcatccggt acatgccgac
      601 gttcgtgatc ttcgtcgcgg gatcgacggt gcagaccatg gggagggtga tgaaccggcc
      661 gccgtcggcg ggccagcatt tgagcacagg gatcttcgaa aggtcgacct gctctccggt
      721 caggacgatt tcctggcacg gggccttttt cacggtcttg gggaaaaaat tggcaagttc
      781 ggccagcttc ggcaaggtct gcagcttgtc cataaagccc gcgcccggcc cggggaagag
      841 gttcgtgggg tcgacgatat ccttgatccg tttgccgatg tcgtcgaggt ccgcaacctc
      901 caggctcatg cacatgcgct tcatcgaacc gaaggcgttc gtaaggacgg ggaacctcga
      961 atccttgacc ctctcgaaga aaagggcctt gttttccgtc cccttctgct tggacacgcg
     1021 gtccgtgatc tccgcaatct ccaactccgc gtccacttcc gcttttatgc ggaccaattc
     1081 cccgttcttt tcaagaacgg cgatgaaatc tcttaatgat tcgtatgcca taggggaaga
     1141 attgtaacgg aattcagggc tctttgcaat caaaaactcg ggtgagccgc acgatacctg
     1201 tacgctaaag cgttcgaata agccagcccc actggatgag caggagtacg atcgccacgg
     1261 atctgacggc gtttttttca cgctccgtaa ggatgaaacc gattcttcgt gaatcgaata
     1321 gaagcgtgat caggctgtag aaaaagaaga ggacggcgaa aaccatggcg atcgagatga
     1381 gagggttcat ggccagtgcg aaagcgattt cgccatgcga cagatgcacg acggccctcg
     1441 tggcaccgca ggttgggcag ggaatatcga aaaggccctt gaaggcgcag gaaggcgaaa
     1501 gtaacctgac cgggagaaac cacggtacag caagtgccgc gacggcgatc ccgccgtaga
     1561 tgatgccgaa ctcaatcccg ccgcgctctc gttttttgaa ggtgacttgc atacgcggtc
     1621 ttccgccttc tgctccatat tctgaacgga gcctgctccc cgaatgctcc tatcgggaaa
     1681 actccgaact ctgaactgta tttatcacgg gtaccaatgc atcatgccgc cgaaggatgc
     1741 cgctacggcg cccatgaaga gcgttatggt catcgtgatg attccgcagc agagcatgaa
     1801 gggaaacagg accgcaacgg ccgccttacc gccggaagtc tcgtgcgctt cccggagtcc
     1861 gatgatggaa agcgttatga cccacagcca cgtgaccggc atgccgcaga agggaataat
     1921 catgaccacg aagggcgacg tgctgtatcc caccacccgg aacgtggcct cgaatccggc
     1981 ctttgcgccc cgcaccatga gaagcataag gtgaagaaaa ccggaggtta aaaaaagacc
     2041 gataatgagg ataaaagggg tcagaacgct gaaataagag aagccgcggt catacatcat
     2101 gcggcgctcg gaccacgccc gaaattcagg ggcaacgaaa tgctgcagcg aatcgtagaa
     2161 caggatgtcc cacagatagg aaaacagcag gcccgtcatg ccgacaatca tcgcgaagag
     2221 cagcgggtcc gtaaggcctc cggtcacagg catcttccgg aaaaagtctg tcggcgtaaa
     2281 caggacttcc ttgagggtct tgagcag
//
LOCUS       DASSLD010000611         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1124645, whole genome shotgun sequence.
ACCESSION   DASSLD010000611 DASSLD010000000
VERSION     DASSLD010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124645"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="VFK23_11005"
     CDS             <1..1449
                     /locus_tag="VFK23_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HET7319655.1"
                     /translation="DDRRMQALLIAFSFGSFIEGAAGFGTPVAITAAMLAGLGFNPLY
                     AAGVCLVANTAPVAFGAIGIPIIVGAKVSGVDEMALSQMVGRTLPFLSVLIPLYLTIL
                     MAGWKKGLEVWPACLVSGLSFALVQFTSSNYLGPMLPDILASVVSIICLTLFLRIWHP
                     KKSWTFDHEAAATGREKLRYTGGQVFRAWAPFIILSIFVAAWGVKPIKAALDYYGLVK
                     FQIPELHNMVIRGGKPMEAVYSFTILSAAGTAILFAGIFSVPVMGASVKTALAVAGET
                     LRKLKFPILTIATILGFAYIMNFSGMAITLGNAFASTGALFPFFAAFLGWLGVFMTGS
                     DTSSNALFGKLQAVTADKIGIDPVITVGANSSGGVCGKMISPQSLSVATAASGLVGRE
                     SDIFRFTLKHSVILTAVIGVMAYLQAYAISWIVPSYVKVAAAVPAAAAAAKPAVPALP
                     TDGLIYLVAIVTVVLSIVVLSRTTGKDVVTAE"
     gene            1511..>2459
                     /locus_tag="VFK23_11010"
     CDS             1511..>2459
                     /locus_tag="VFK23_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET7319656.1"
                     /translation="MLHTSVADKLEAIVGKDDLFTKKADLLAYGYDAAAGAGMTRRTP
                     DLVVLPSSTEEVQRIVHLAREARLPIYPRGAGTNLSGGATPLQGGIVLSLQRMDRILE
                     VDPENLTATVQPGVIIQDLNNAAAAHGLLYPPDPGTVATATMGGSVAECSGGLRGLKY
                     GVTKHYVMGMEVVLASGETFRFGGKTVKNVTAYDFASLFVGSEGTLGIITEITVKLIP
                     APKFRRAMMAFFRRLEDAGNAVTDIIKAHVIPATLEIMDRTTVATVERYAKAGLPADV
                     EALLLIEVDGMAQEAVDAEAQAVAAVVGRHGGALRTASDD"
BASE COUNT          429 a          785 c          760 g          485 t
ORIGIN      
        1 gacgaccgaa gaatgcaggc tctgctgatt gccttctcct tcggctcgtt catcgagggc
       61 gctgcaggat tcggcacgcc cgtcgccatc acggccgcga tgctggcggg cctcgggttc
      121 aacccgctct atgctgcggg cgtctgcctc gtggccaaca cggcgcccgt tgccttcggc
      181 gcgatcggga tcccgatcat cgttggtgca aaggtatcgg gtgtggacga gatggctctc
      241 tcccagatgg tcggccgcac gttgccgttt ttaagcgtcc tgattccgct ctacctcacg
      301 attctcatgg cgggatggaa gaagggcctc gaggtatggc ccgcctgcct ggtgagcggc
      361 ctgtccttcg cgctcgtcca gttcacctcg tccaactacc tcggccccat gctccccgat
      421 atccttgcat ccgtcgtttc catcatctgc cttacgctct tcctcaggat ctggcacccg
      481 aaaaaaagct ggactttcga tcacgaggcc gccgccacgg gcagggagaa actgcggtac
      541 accggcggac aggtcttccg cgcctgggcg ccgttcatca tcctatcgat cttcgtggct
      601 gcttgggggg tgaagccaat taaggcggca ctcgactact atggcctcgt gaagttccag
      661 attcccgagc tccataacat ggtcatccgc ggcggcaagc cgatggaggc ggtctacagc
      721 ttcacgatcc tgagcgcagc cggtacggcg atcctcttcg cgggaatctt ctcggtcccg
      781 gtcatgggtg cgtctgtgaa aacggcgctc gccgttgcgg gggagacgct ccggaaactc
      841 aagttcccga tcctcacgat cgcaacgatt ctgggattcg cctacatcat gaacttctcg
      901 ggcatggcga tcacactcgg aaacgccttc gcctcgaccg gcgccctctt cccgttcttc
      961 gcggccttcc tcggctggct cggcgtgttc atgaccggct ccgacacgtc gtcgaacgcg
     1021 ctcttcggaa agcttcaggc agtcacggcc gacaagatcg ggatcgaccc ggtcatcacc
     1081 gtgggcgcca attcctcggg cggggtttgc ggcaagatga tctctcccca gtccctgtcc
     1141 gttgcaaccg cggcgtcggg ccttgtgggc agggaatcgg acattttccg tttcacgctt
     1201 aagcattcgg tcatcctgac cgcggtgatc ggggtcatgg cttacctaca ggcctacgcc
     1261 atatcctgga tcgttccgtc ctatgtcaaa gttgcggctg ccgtcccggc ggccgctgcg
     1321 gcggccaagc cagcggtgcc ggcgctccca accgacggac ttatctacct ggtggccatc
     1381 gtgacagtgg tcctctcgat cgtcgtcctt tcaaggacaa ccggcaagga cgtggtgaca
     1441 gcggagtaaa catgatcagg gggcgccggg accttaccgg cagttgcctc ttcttttgag
     1501 ggagaatggc atgcttcata cgagtgtcgc cgataaactt gaagcgatcg tcggaaaaga
     1561 cgacttgttc accaaaaagg ccgatctact tgcctacggc tatgacgccg ccgcgggcgc
     1621 gggaatgacc cgcaggaccc ccgatctggt cgtccttccc tcctcgacgg aggaggtgca
     1681 gcgcatcgtg caccttgccc gtgaagcgcg gttgccgatt tacccccgcg gggcgggaac
     1741 caatctgagc ggcggggcaa ctcccctgca gggagggatc gtcctctcgc tccagagaat
     1801 ggaccggatt ctcgaggtgg acccggagaa cctcacggcc acggtccagc cgggggtgat
     1861 catccaggac ctgaacaatg ccgcggccgc ccatggactc ctgtatcccc ctgaccccgg
     1921 cacggtcgcg accgccacga tgggaggttc cgtcgccgag tgctcggggg ggctgcgggg
     1981 gctcaaatac ggggtcacga aacattacgt catgggcatg gaagtagtcc ttgcgagcgg
     2041 ggagaccttc cgcttcggag ggaaaaccgt aaagaacgtc acggcctacg atttcgcctc
     2101 tctcttcgtc ggctccgagg gcacgctcgg aatcatcacg gagattacag tgaagctgat
     2161 tcccgcgccg aagttccgcc gcgccatgat ggcgttcttc aggcgtctgg aggacgcggg
     2221 gaacgcggtg accgatatca tcaaggcgca cgtcatcccc gcgacgctcg aaatcatgga
     2281 caggacgacc gtcgccaccg tggaacggta cgcgaaggcc ggcctgcccg ccgacgtgga
     2341 ggccctgctt ttgatcgaag tggacggcat ggcgcaggaa gcggtcgatg ccgaggcgca
     2401 agcggtcgcc gcggtcgtcg gcaggcatgg cggcgccttg aggaccgcct ccgacgacg
//
LOCUS       DASSLD010000612         3487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_534665,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000612 DASSLD010000000
VERSION     DASSLD010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534665"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VFK23_11015"
     CDS             complement(<1..897)
                     /locus_tag="VFK23_11015"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HET7319657.1"
                     /translation="MKRQGYATRPLLKGVLWGAGILAALLLFLFVASYLLDEPLRGSM
                     EKRINAPLKGYSVRLSGLHLQLIGFSLTLNGLTVFQQANPDPPIARFPVLHFGLHWRE
                     ILLGKVVAEVELEQPEVRIDLRQYRTEAASPVPLKERGWQQAVESIYPFKINALTIRD
                     ATLTYIDQDPEKPLRLTHMNLVASNIRNVRFPSNVYPSSFRMETAIFGVGRGIVEGNA
                     NFLMEPHLGINARLTLENVPLEYFKPVVARTNLNIRSGTFTGSGRIEYAPNVKVTHLG
                     DLTIQGMEIDYVHTARTAEIEKR"
     gene            complement(1197..1616)
                     /locus_tag="VFK23_11020"
     CDS             complement(1197..1616)
                     /locus_tag="VFK23_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HET7319658.1"
                     /translation="MDPLTAIAERRIQEAIERGEFDDLPGKGKPLDLGEDDRTVPAEL
                     RMAYRILKNAGLLPPDVELHKEILRLTDLIDSIHDEEGRRERRRELDYKLMKLNMMRR
                     RPVNLEILPEYRDRVEEKLSHRGGSARRTLAERPGRN"
     gene            complement(1796..2134)
                     /locus_tag="VFK23_11025"
     CDS             complement(1796..2134)
                     /locus_tag="VFK23_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319659.1"
                     /translation="MKKGKMQSGILVLGTGLIIGALIAVSVPPEVSAATPCCTITSID
                     MKTGILTAKDTATGETFEFKLGNPAKISNIKIGDQVSTDFRNREVTVHSFQPVDGIIV
                     TAPMPRPKIK"
     gene            complement(2317..3021)
                     /locus_tag="VFK23_11030"
     CDS             complement(2317..3021)
                     /locus_tag="VFK23_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319660.1"
                     /translation="MDTAASPRRLIAILGGMYSRELRIDLSGPDHAPIFRWFLAAKLM
                     GARISTRAALSTWGEFDRRGIDTPQRIRQAGWDGLVAILDNGGYARYDFSTATRLLAI
                     AGNILERYHGDLNILHARSEGPRDLEARLMGLGRGIGPVTVNIFLREMRPVWPKADPD
                     LSPLALLAARDLGLVKGKGNPLDALRLAWAAEKARGYNFSDFEAALVRLGKDYCRKPR
                     VTNCPMRGICPRLRSA"
     gene            complement(3085..>3487)
                     /locus_tag="VFK23_11035"
     CDS             complement(3085..>3487)
                     /locus_tag="VFK23_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET7319661.1"
                     /translation="LPAVVPPGPENPMGSHALRLSKPTVLIHGTNRPWGIGARVSHGC
                     IRLYQEDIARLFEMIQRGTPVIIVNQPVKTATIGDRVYLQVQDYEEGGRDLYDEALKV
                     LEAKNLTNWVDLMKIRKASQERTGLLVDISR"
BASE COUNT          684 a         1025 c         1002 g          776 t
ORIGIN      
        1 tcgcttttcg atttccgccg tccttgccgt atggacgtaa tcgatctcca tcccctggat
       61 cgttaggtcg ccgagatgag tgaccttgac gttgggggcg tattcgattc ggccggaacc
      121 ggtgaacgtc ccgctccgga tgttgagatt cgtacgggcg accacaggtt tgaaatattc
      181 gagcgggacg ttctccaacg tcaggcgggc gttgatccct aggtgcggct ccatgagaaa
      241 attggcgttg ccttcgacga ttccgcgccc gacgccgaag atcgccgttt ccatacggaa
      301 cgacgacgga tagacgttgc tggggaagcg gacgttgcgg atattgctcg ctaccaggtt
      361 catgtgggtc aggcggagag gcttttccgg gtcctgatcg atataggtaa gcgttgcgtc
      421 acggatcgtc agggcgttga tcttgaaggg atagatcgac tccaccgcct gctgccaacc
      481 gcgctccttg agcggcaccg gactggcggc ctcggtacga tattgccgaa ggtcgattcg
      541 gacttccggt tgttcgagct cgacttccgc gacgaccttt ccgaggagga tttcccgcca
      601 gtgaaggccg aagtgaagaa ccggaaagcg ggcgatcgga ggatccggat tcgcctgctg
      661 gaaaacggtc aacccattca gggtcaggga aaacccgatc agttgaagat gcaaccccga
      721 cagtcggacg gaataccctt tcaggggagc gttgatcctt ttctccatgg atccgcgcaa
      781 cggttcgtcc aggaggtagg aagcgacgaa aagaaacaga aggagggccg caaggatgcc
      841 tgcgccccag agcacccctt tcagcagcgg ccgagtcgcg tatccttgtc gtttcattgg
      901 ctttccggtg gaatcggaat cctcagcgcc cttcccggtc attcctaata agaataagaa
      961 ggttcaccca cctcgatccg atcgacgcac cacagttcct gctcatttca tgggacggtg
     1021 cgttcttgga gcaggtttca catcgaagat cattcccggg gagatttccc ggtctggcag
     1081 tcccatctct ttaatcagtg ggctgtaaac catacgcatg ccgggggaat gggagaaatc
     1141 gcacccgcgt agatcacgga aaaaaggcgc ctgacagcgc ctgaggcata cggatattaa
     1201 tttcggccgg gacgttcggc caatgtccgt ctcgcggagc cacctctgtg tgacagcttc
     1261 tcctcgactc ggtcccggta ttcaggcaat atttcgaggt tgacgggcct ccggcgcatc
     1321 atgttgagct tcatcagctt gtagtcgagt tcccgacggc gctcgcgacg accttcctca
     1381 tcgtgtatgg agtcgatcag atccgtcagc cgcagaatct ccttgtgaag ctcgacatcc
     1441 ggggggagca ggccggcgtt tttcagtatc cgatacgcca tccgcagctc cgcagggacc
     1501 gtccggtcgt cctccccgag atccagaggc ttccccttac ccgggaggtc gtcgaactcg
     1561 ccgcgctcga tcgcctcctg gatccgacgc tccgcgatgg cggtcaacgg gtccatgtca
     1621 tttatgatac ttccatgata tgccgtttgg cgacttcgtg cgcacatcca cccgttcctt
     1681 cagaagatgt tagcggaact tctccattga gccggtttcg gtttttggga agtcgacaac
     1741 tatcagcggg aatccctctt gtgcggttga aaagggttca gggggacacg ggttttcact
     1801 taatcttcgg ccgaggcata ggcgccgtta cgatgatccc gtctaccggc tgaaagctgt
     1861 gtacagtcac ctccctgttc cggaaatccg tcgaaacctg atcgcctatc tttatgttac
     1921 tgatcttcgc cggattgccg agtttgaatt cgaaggtctc cccggttgcg gtatccttcg
     1981 cggtcaagat gccggtcttc atgtcaatgc tggtgatagt gcagcaaggg gttgcggcac
     2041 tgacttcagg gggaacggag acggctatca atgctccgat gataagcccc gttcccaata
     2101 ccaagatccc tgactgcatc tttccctttt tcatgatttc ccccttagat agaaggttac
     2161 aaagagttta gcactggtcg atggatccga accctccggc gccctttcct gccataggac
     2221 aaacgccggg ctgagaatca gacaaacggc gaccccccaa ttgattgttc gcccagtaga
     2281 cctggccgca ccgggcgcat gtcctgttgc ggaaaatcag gcggaccgaa gtctggggca
     2341 gattccccgc atcgggcagt tggtcacgcg gggcttccgg cagtaatcct tgccgaggcg
     2401 aacaagcgct gcctcgaagt cgctgaaatt gtatccgcgc gctttctccg ccgcccaagc
     2461 taacctcaaa gcgtcaagag gattcccttt ccctttgaca aggccaaggt cccgtgccgc
     2521 cagcaaggct agaggggaga ggtcgggatc ggccttgggc cacacgggcc gcatctcgcg
     2581 caggaatata tttaccgtca ccggcccgat ccccctcccc aggcccatga gtcgggcttc
     2641 caggtccctc ggtccttccg accgggcgtg gaggatattc aggtccccgt gatatcgctc
     2701 caggatgttg cccgcgattg cgagaagcct ggtggcggtg gaaaagtcgt accgggcgta
     2761 ccccccgttg tccagaattg ccacgaggcc atcccaaccg gcctgtcgga ttctctgcgg
     2821 ggtatcgatt cctcgccggt cgaactcccc ccaggttgag agcgctgccc tcgtcgagat
     2881 cctcgcgccc atcagcttgg ctgccaggaa ccagcggaag atcggcgcgt gatccggtcc
     2941 cgagaggtcg atcctgagct cgcgggaata catgccacca aggatcgcta tcagccgtct
     3001 tggcgacgcg gcagtgtcca cggagggatc tccctgggga taagaaaaca aaagattaat
     3061 atagagggta ggcgttaatc tcctttatct cgagatgtcc accaggagac ctgttcgctc
     3121 ctggctcgcc ttccggattt tcatgagatc gacccagttt gtcaggtttt tcgcttccag
     3181 caccttcagc gcctcgtcgt acagatccct tccgccttcc tcatagtctt ggacctgcag
     3241 gtagacacga tctcctatgg ttgccgtctt cacgggctgg ttgacaatga taaccggcgt
     3301 cccccgttgg atcatttcga acagccgggc gatatcctcc tggtatagtc tgatgcatcc
     3361 gtgactgacg cgcgccccga ttccccaggg acgattcgtg ccatgaatca gcacggtcgg
     3421 cttcgagagg cggagcgcgt ggctccccat cggattctcc gggccgggag ggacgacggc
     3481 cgggaga
//
LOCUS       DASSLD010000613         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1409744, whole genome shotgun sequence.
ACCESSION   DASSLD010000613 DASSLD010000000
VERSION     DASSLD010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409744"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /gene="pstS"
                     /locus_tag="VFK23_11040"
     CDS             <1..665
                     /gene="pstS"
                     /locus_tag="VFK23_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET7319662.1"
                     /translation="LDSETICKIYLGEIKTWNDPKIKGLNPGVNLPANGITVVHRSDG
                     SGTTAIFTDYLSDVCPAWKEKVGAGTSVSWPAGIGGKGNEGVANYVQQQANSIGYVEF
                     AYAKQNRMVATRLKNKAGSFVEPSFESFEDAAATASFDPKKDFYLWLNDAPGKKAWPI
                     AGATFILLAKEKKDSNMKVVKFYDWAFKNGDAKAKELIYVPLPKSLKAKVEGYWKTNG
                     IH"
     gene            776..1717
                     /gene="pstC"
                     /locus_tag="VFK23_11045"
     CDS             776..1717
                     /gene="pstC"
                     /locus_tag="VFK23_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET7319663.1"
                     /translation="MTIFPKKNIVDLLFSALTALASFSVISFIIGIIVTLIMESRLAI
                     GKFGVFDFLTSTAWNPVTGVFGAATTIDGTFVTTGLALLFALPTALGIAIFVTEIAPN
                     FLKGPIGAAIELLAAIPSIIYGMWGLFTLAPIMSKYIEPALQNTLGKLPVVGILFTGN
                     PMGIDLLTASVILAIMIVPFTASIARDSFNLTPAVVKESAYAVGATKWEVIKNVVLPY
                     SKLGVFGGVALSLGRALGETMAVAFVLGNNHKIATSLLDAASTITVSLANEFTEADSD
                     LYLSSLYYLALVLFVMSFIILAISKYFLLKAERKYSR"
     gene            1894..>2154
                     /locus_tag="VFK23_11050"
     CDS             1894..>2154
                     /locus_tag="VFK23_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HET7319664.1"
                     /translation="MNVFNILISGVGGQGVLLTSKIIAEAALRSGLDVKQSEVHGMAQ
                     RGGSVLSQVRFGEKVFSPIVSEGEADLLIGFEPLETARYLHFL"
BASE COUNT          474 a          613 c          606 g          461 t
ORIGIN      
        1 gcctcgattc ggaaacgatc tgcaagattt acctgggcga gatcaagacc tggaatgatc
       61 cgaagatcaa ggggctgaat cccggcgtca accttcctgc aaacgggatt accgtggtcc
      121 atcgctccga cggctcgggc acgaccgcca tcttcaccga ttatctctcc gatgtctgcc
      181 cggcctggaa ggaaaaggtc ggcgccggca cgtcggtgag ctggcctgcc gggatcggcg
      241 gcaagggaaa tgaaggagtg gcaaactacg ttcagcagca ggcgaactcc atcggctacg
      301 tggaattcgc ctatgcaaag cagaacagga tggttgccac caggctcaag aacaaggcgg
      361 gctccttcgt ggagccgagt ttcgagagct tcgaagacgc tgccgcgacc gccagcttcg
      421 acccgaaaaa ggacttctac ctctggctga acgacgcccc gggaaagaaa gcctggccca
      481 ttgcgggcgc cacgttcatt ctcctcgcaa aggagaaaaa ggattcaaac atgaaggtgg
      541 tcaagttcta cgactgggcg ttcaagaacg gagatgccaa ggccaaggaa ctcatctatg
      601 tgccgctccc gaaatcgctc aaggccaagg ttgagggtta ctggaagacg aacggcatcc
      661 attaaacagt aatagaaaaa atttgaacaa agcgggcctg gcatatatac tatagatgat
      721 gcccggcccg ttcaagtttt accgaatacc ggtgcagcga agaaaggtcc gctccttgac
      781 cattttcccc aaaaagaaca tcgtcgacct ccttttttct gcgctgaccg ccctcgcttc
      841 attttccgtc atcagcttca taatcggcat catcgtcacg ctcatcatgg aatcccggct
      901 cgccatcggg aagttcgggg tcttcgattt tctgacctcg acggcctgga accccgtgac
      961 cggggtcttc ggggccgcga cgaccattga cggaaccttc gtcacgacag gccttgccct
     1021 gctcttcgca cttccgaccg ccctcggcat tgcgatcttc gtcaccgaga tcgcgccgaa
     1081 tttcctcaag ggccccatcg gggccgccat agaactcctg gccgccatcc ccagcatcat
     1141 ctacggcatg tggggactgt tcaccctcgc ccccatcatg tcgaaatata tcgagccggc
     1201 gcttcaaaat accctgggga agctgcccgt ggtcggcatc ctgtttacgg gcaatcccat
     1261 gggaatagat ttattgaccg cgagcgtcat ccttgccatc atgatcgttc catttacggc
     1321 aagcattgcg cgcgactcat tcaatctgac cccggcagtg gttaaggagt cggcctatgc
     1381 cgtgggggca accaagtggg aagttatcaa gaacgtggtg ctgccgtact cgaagctggg
     1441 cgtcttcggc ggggttgcgc tttccctggg cagggccctg ggcgagacca tggccgtcgc
     1501 cttcgtgctc gggaacaacc acaagatcgc cacgtcactc ttggacgcgg cgtccacgat
     1561 cacggtgagc cttgcgaacg aatttacgga agcggacagc gacctgtatc tttcgtcgct
     1621 ctattacctg gcgctcgtcc tgttcgtgat gagcttcatc atcctggcga tatcgaagta
     1681 ctttctgctg aaggcggaga ggaagtattc gaggtgaaaa gctttttaac cacggagaca
     1741 cggagacacg gaggaagttt taataataag atattgaagg acaatttagg gtgtcattcc
     1801 cgcgatggcc gatcaagcgg gccacgctga agcgggaatc cagtgtcttt caccgtgttc
     1861 atccgtggtt aactatttct gaggtgttta tctgtgaacg tatttaatat cctcatcagc
     1921 ggcgtgggcg ggcagggggt tctgctcacc agcaagatca tcgccgaggc ggcgctccgc
     1981 tccggcctcg atgtgaagca gagcgaggtg cacggcatgg cccaacgcgg agggagcgtg
     2041 ctctcgcagg tgcgcttcgg ggaaaaggta ttttcgccga tcgtatccga aggcgaggcc
     2101 gaccttctga tcgggttcga gcccctggag accgcgcgct accttcattt tctc
//
LOCUS       DASSLD010000614         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1317306, whole genome shotgun sequence.
ACCESSION   DASSLD010000614 DASSLD010000000
VERSION     DASSLD010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317306"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VFK23_11055"
     CDS             <1..733
                     /locus_tag="VFK23_11055"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET7319665.1"
                     /translation="FNEPVAAASVSDVTFTLSGGVTGRVTYDPKTKTATFTPDRPLSF
                     NHTYTITISAGVMSLGNVPMSSEFTSTFTTNAAPAAPHLYSPTDGSVIIGPAVSLQWI
                     ASTDSDAENVTYHLFYCTNPSMTGCNPVDVSSSATTTTASAFRNPQSIGGFGAGLVLA
                     GFAIIGGVTSRRKIFFIVAALLITGSAVAACGKKSSEPPAPPAGLTTKSISELQAGTR
                     YWWKVVADDGNGGTAESETWSFTTQ"
     gene            842..2023
                     /locus_tag="VFK23_11060"
     CDS             842..2023
                     /locus_tag="VFK23_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319666.1"
                     /translation="MKILGILGSILGIAIVTVTACSHHTEPAADVRPKLPQPTTVVNG
                     LASMGPVNSGFVDIFAVRGGVIDASTPIGRGITDANGLFSGDLGAYTGTVMVMISQGS
                     FTDEVSGVRVALKQPMQAMASLVSTGQNTVAVTPLTDLAVAAGRGLATPEAVDDANNK
                     ISALFQVGDIIKTVPNAGGTADQKKYADACGVISQLANDRSTQKGEPLDDALVAVMSS
                     LESELDQSHEFSSDTLAGINAAVAEFNKNGAAAAALLPSAGTLKISTAGPTNMIGTVN
                     MTVTLPDGAAVASDPLTGEALQGAVMVSGEAAIGSNTLLTANVIPAAGSAPAQVVIRM
                     TNSTGFGIGECVTINFTRTAAGTYPANAAAFVLTGVAVKGPNGLPLAGVTIVPTGLSG
                     L"
     gene            2171..>2409
                     /locus_tag="VFK23_11065"
     CDS             2171..>2409
                     /locus_tag="VFK23_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319667.1"
                     /translation="MNANRLVVLILSLAVLSLIGCRHKTEPLPPATLIIGVASAGPIN
                     FGLVTAYAIRSGVVDTSSPLGQEPTDPDGNFAVNVG"
BASE COUNT          517 a          701 c          721 g          470 t
ORIGIN      
        1 cttcaacgaa ccagtggctg cggccagtgt gagcgatgtc actttcacac tctccggcgg
       61 ggtcacgggc agggtgactt atgatccaaa gaccaagacg gcgaccttca cgcctgacag
      121 gcccctgtcg ttcaatcata catatacgat aacgatcagc gcgggtgtga tgagccttgg
      181 caacgtgccc atgtcctccg aattcacctc gacgtttacc acgaacgccg ccccggcggc
      241 cccgcacctc tattcgccga cagatgggtc ggtaataatc ggccccgccg tgagcctgca
      301 gtggatagcg tccacggact cggatgccga aaacgtaaca tatcacctgt tttactgcac
      361 caacccatcc atgacaggct gcaacccggt tgacgtttca tccagcgcta ctacgacaac
      421 ggcctccgca ttccgcaatc cgcaatcgat tggcggcttc ggagcgggac ttgtccttgc
      481 cgggttcgcg atcatcggcg gggtcacctc gcgaagaaag atatttttca tagtcgcagc
      541 gctcctgatt acgggatcgg cggtcgccgc gtgcggcaag aagagcagcg aaccgccggc
      601 accgcccgcg ggtcttacaa caaagagcat cagcgagctg caggccggaa ccaggtactg
      661 gtggaaagtg gtcgctgacg acggaaacgg cgggacagcg gagagcgaga cgtggtcgtt
      721 tacgacgcaa taggattgtt gatggatgat tgatgatcgt tgattgaaaa gaaaaaacct
      781 aagcctgtag tcttttattc atcaatcaac gatcaataat cgacgattaa gtgaggtcgt
      841 catgaaaata ctaggcatcc ttgggtccat cctgggtatc gcgatagtga cggttaccgc
      901 ctgcagccat cataccgagc cggcggcgga cgtgcggccg aagttgcccc agccgaccac
      961 ggtagtgaac ggccttgcct cgatgggacc ggtcaactcc ggttttgtag atattttcgc
     1021 agttcggggc ggcgtcatcg acgcgtccac ccctatagga cgcggtatta cggatgcgaa
     1081 cggacttttc tccggcgacc tcggcgccta caccggaacg gtgatggtga tgatcagcca
     1141 gggttcattc acggacgaag tgtcgggcgt ccgcgtcgcg ctcaagcagc cgatgcaggc
     1201 aatggcctcc cttgtctcaa cagggcagaa taccgtggcc gtgaccccgc tcaccgatct
     1261 cgccgttgcc gcgggccggg gactggcaac gccggaggcg gtagatgatg caaacaacaa
     1321 aatatcggcc ctgttccagg tgggcgacat cataaagaca gtgccgaatg cgggcgggac
     1381 cgcggaccag aaaaagtacg cggatgcatg cggggtcata tcgcaactgg ccaatgacag
     1441 gagcacgcaa aagggagaac cgttggacga cgcccttgtt gctgtcatga gcagtctcga
     1501 atcggagctt gaccaaagcc atgaattctc gtcggacacc ctggccggaa tcaacgccgc
     1561 agtggcggag ttcaataaaa acggggctgc tgcggccgcg ttgctcccct cggcaggaac
     1621 actgaagata agcaccgccg gacccacgaa catgattggt acggtcaata tgacagtcac
     1681 cctccccgat ggggccgcag tggcctccga cccgttgacg ggggaggctc tgcaaggagc
     1741 ggtgatggtc tccggcgagg cggcaatcgg ctcgaatacg cttttaaccg cgaacgtcat
     1801 cccggcagcc gggtcagccc cggcgcaggt ggtaatccgc atgaccaatt cgaccggttt
     1861 tggcatcgga gaatgcgtga cgatcaactt taccaggacc gccgcaggga cctacccggc
     1921 aaacgccgcc gcctttgttc tgaccggagt tgctgtcaag ggtccgaatg gtttgccgct
     1981 cgcgggcgtg actattgtcc cgacggggct gtcggggttg taaatcgttc ctcccccttc
     2041 aagggggagg ttaggtgggg gatgggttat tcagggcggg acacccttat gttctcaacc
     2101 catccccacc ccagccctcc ccttgaaggg gagggagtaa tctctttctg aggagaacgc
     2161 catgaggttc gtgaacgcca atcgtctggt agtcctgatt ctatctttag ctgtgttatc
     2221 tctcatcggc tgccggcaca aaactgaacc tctgcccccg gcgaccctga tcattggcgt
     2281 cgcgtcagcg ggaccgatca acttcgggct cgtaaccgcg tatgctatca gaagcggcgt
     2341 ggtggacact tcgtcgccgc tcgggcagga accgacggac ccggacggga acttcgcggt
     2401 caatgtcgg
//
LOCUS       DASSLD010000615         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_600356,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000615 DASSLD010000000
VERSION     DASSLD010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600356"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..798)
                     /gene="dut"
                     /locus_tag="VFK23_11070"
     CDS             complement(346..798)
                     /gene="dut"
                     /locus_tag="VFK23_11070"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET7319668.1"
                     /translation="MPDHLVVHITQLPNSAGLPLPRYQTAHAAGVDLYAAVEAETVIG
                     AGKWSLIPTGIALAIPEGYEGQVRPRSGLALKHRIGMLNGPGTIDADYRGEIGIILFN
                     FSGEPFTVRRGDRIAQLVFAKLEKARFESVASLSGTARGDGGFGHTGI"
     gene            complement(895..2151)
                     /locus_tag="VFK23_11075"
     CDS             complement(895..2151)
                     /locus_tag="VFK23_11075"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7319669.1"
                     /translation="MYRKDTLSNGIRVVSETLPKSRSVSIGVWVKVGSRHEPPAIGGI
                     SHFIEHMFFKGTGKRSAKDIAIEMDSIGGEMNAFTSQETTSYYAKVVDEHLPVVIEIL
                     ADILLGAKFDPVEMEKERKVILEEIKGVEDTPDDYIHELFTNTVWPDNSLGRPILGTR
                     ETIKELKHNDIVSYIDSYYHPKEIVISVAGNFEHARLIGLLDLHFGKLARNGTAKKEV
                     TPAFTRAVSVKKKQLEQVQLCIGCKGLNYTNEDRFVISALNTVLGNSMSSRLFQEVRE
                     QNALAYSIYSYVTSYRDTGLLTVYAGTDPSNALEVVRLVVKEMKNIKEEGITPAEELR
                     VKNQIKGSLILSLESSNSHMSRLARQEIYFGKYLSMDDIIKGVDKVTAEQVQRLAQQL
                     FTGENTSLTILGPLSKSDVPDEVLEI"
     gene            complement(2263..>2886)
                     /gene="fusA"
                     /locus_tag="VFK23_11080"
     CDS             complement(2263..>2886)
                     /gene="fusA"
                     /locus_tag="VFK23_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512966.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET7319670.1"
                     /translation="QTGGHGQYGDAWLEIEPLPRGSGFEFVDNIVGGVVPRQYIPAVE
                     KGVVEALHEGVLAGYPVVDIRVSLYDGSYHTVDSSEMAFKVAASMAFKKAVEAAKPVL
                     LEPIMSVEVISPDDTLGAVIGDLNSRRGKVQGVVPQANGQTIKALVPLSEMLTYAPTL
                     NSLTSGRGMYTMEFYGYEDVPSHLAQKITQERAAQLHGGAAHNQKEK"
BASE COUNT          546 a          855 c          842 g          643 t
ORIGIN      
        1 gcgagatgag caaccgctcc cagttcatca cgatcacgca caacaagcgg accatggaga
       61 tggcggacgc cctctatggg atcaccatgg aagagcccgg atgctcccgg gtcatttccg
      121 cgaagctgcg ggaaccggcg ctggtgtagc gacagttcgg agttcggagt acaaattttt
      181 ttatcttgtc atacctgcga aagcaggtat ccattggctt ttttgtgaca aacttcaaaa
      241 tggattcccg ctttcgcggg aatgacaaaa aagatttatg gcgccatttg tactcgtttt
      301 tttcgctctt cattccgcat ttcgaaatcc gcattccgca tttcatcata tccccgtatg
      361 cccgaacccg ccgtcgccgc gcgcggtgcc ggacaacgac gccacgctct caaacctggc
      421 tttttcaagt tttgcgaaca ccagctgggc gatgcggtcg ccgcggcgga ccgtaaaggg
      481 ttcgccgctg aaattgaaca ggatgattcc gatctctccc cggtagtccg cgtcgatggt
      541 gcccggcccg ttcagcatgc caatgcggtg cttgagcgcg agcccgctcc gcggccggac
      601 ctggccttcg tacccttccg ggatcgccag ggcgataccg gtggggatga gcgaccactt
      661 ccctgccccg atgaccgttt cagcctccac tgccgcatag agatcaacgc cagccgcatg
      721 ggccgtctga tagcggggta gaggcagccc agcggaattc ggcagttgcg tgatatggac
      781 gacgagatgg tcgggcatat tcgtaatatt tgcaaagagc agagcgcaaa gcgcatagcg
      841 aaaaaaaagc ctgcacagaa agcacctgcc ctaagcgcta tgcgctatgc ggaatcaaat
      901 ctccaaaacc tcatcgggca cgtcggactt gctgagcggc cccaggatcg tgagcgaggt
      961 gttctcgccg gtgaagagct gctgcgccag ccgctgcacc tgctccgccg tgaccttgtc
     1021 cactcccttg atgatatcat ccatggacag gtatttgccg aaatagatct cctgccgcgc
     1081 gagcctgctc atatggctgt tgctgctctc cagcgaaagg atcaggctcc ccttgatctg
     1141 gttcttcacc cggagttcct ccgcgggggt gataccctct tccttgatat tcttcatttc
     1201 cttgaccacc agcctgacca cctcgagggc gttcgacgga tcggtccccg cgtacaccgt
     1261 gagcagtccg gtgtcgcggt aggacgtgac gtaggaatag atcgaatagg caagggcgtt
     1321 ctgctcccgg acctcctgga acaggcggga gctcatgctg ttgccgagca ccgtattgag
     1381 cgccgagatc acgaagcggt cctcgttggt gtagttgagc cccttgcagc ctatgcagag
     1441 ctgcacctgt tcgagctgct ttttctttac ggatactgcg cgggtgaacg ccggggttac
     1501 ctcttttttg gcagtgccgt tccgcgccag tttcccgaaa tgaaggtcca gcagtccaat
     1561 aagccgtgcg tgctcgaaat ttcccgcaac ggatatcacg atctccttcg gatgataata
     1621 gctgtcgatg taggaaacga tgtcgttgtg cttgagctcc ttgatggtct cccgggtccc
     1681 caggatgggt ctgccgagtg agttgtcggg ccagaccgtg ttcgtgaaga gttcgtggat
     1741 gtagtcgtcg ggggtgtcct cgactccctt gatctcctcg aggatgacct tgcgctcctt
     1801 ttccatttcg acggggtcga acttcgcgcc gaggaggatg tccgcgagga tctcgatgac
     1861 gaccggaagg tgctcgtcta cgactttggc gtagtaagag gtggtctcct gcgaggtgaa
     1921 ggcgttcatt tctccgccga tggagtccat ctcgatggcg atgtccttgg cggaccgctt
     1981 gccggtcccc ttgaagaaca tgtgctcgat gaaatgtgaa atcccgccga tcgcaggtgg
     2041 ctcgtgccgg gacccgacct tcacccacac cccgatggat acggagcgcg acttcggaag
     2101 ggtctcggac acgacccgga taccgtttga aagggtgtct tttctgtaca tagcgcggtc
     2161 tccggccgca accaaaagcc gttgtaggtt acgggttgcg ggttacgggt tcgaaacaaa
     2221 cgagcgcccc ccaccgcgca actcgcaaca tatctttcgt tgttattttt ccttctgatt
     2281 gtgggcggcc ccgccatgga gctgcgcggc ccgttcctgg gtaatcttct gcgccaggtg
     2341 gctcgggaca tcttcgtagc cgtaaaattc catcgtgtac attccccgcc cgctcgtcag
     2401 ggagttgagc gtgggcgcgt aggtgagcat ctcggacagg ggtaccaggg ccttgatcgt
     2461 ctggccgttc gcctggggga cgacgccctg caccttgccg cggcgcgagt taaggtcgcc
     2521 gatcacggcg ccgagtgtgt cgtcgggaga gatcacctcg acgctcatga taggctcgag
     2581 gaggaccggt ttggccgctt ccacggcctt cttaaaggcc atgcttgccg caaccttgaa
     2641 cgccatttct gaagagtcca ccgtatggta ggagccgtca tacagcgaga ccctaatgtc
     2701 cacgacggga taccccgcga ggacgccctc gtgcagggct tcgacgaccc ccttctcgac
     2761 agcggggata tactgccggg ggaccacgcc gcctacgatg ttgtccacga attcgaaccc
     2821 gctcccgcgt ggcagaggct cgatctcaag ccaggcgtca ccgtactggc catggccgcc
     2881 cgtctg
//
LOCUS       DASSLD010000616         4302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1155023, whole genome shotgun sequence.
ACCESSION   DASSLD010000616 DASSLD010000000
VERSION     DASSLD010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155023"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFK23_11085"
     CDS             complement(<1..443)
                     /locus_tag="VFK23_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319671.1"
                     /translation="MALFRREWDDLARAAVRLRFPADVESAFRRYHSRNAVVITRYAL
                     VLTALLFGLFAILDVYAAPLMWRAIWFIRFAVVIPALAVVLASSFHPRFPRILQPAMV
                     FVVLTDGAGVLAMIDIASHRELGYQTYYAGLMLVIMAAHSIYRLR"
     gene            complement(816..1148)
                     /locus_tag="VFK23_11090"
     CDS             complement(816..1148)
                     /locus_tag="VFK23_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007622916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HET7319672.1"
                     /translation="MESTGPVYALNMFDIVDREEYMAYSRRSAREVAAHGGKLVALGR
                     FREAQKGDITPRQVLILVEWESREAFQSYLDDPAIADLHPHRERGAGNYIWHLFDRLE
                     DLRPILKV"
     gene            1422..1796
                     /locus_tag="VFK23_11095"
     CDS             1422..1796
                     /locus_tag="VFK23_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319673.1"
                     /translation="MGIPAPVVVAPQPVYVEQPPEMVVIPRSNVYFAPGVSVDLFFYD
                     NRWWNRRGDRWYRGSGYNGPWVAVGPRHVPGPVYRVPANYRTVYGHEKPMPYGQWKKA
                     HGEHGGKHRGEMHGNKRGHHDD"
     gene            1896..2300
                     /locus_tag="VFK23_11100"
     CDS             1896..2300
                     /locus_tag="VFK23_11100"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HET7319674.1"
                     /translation="MMASLRKTAWFVMVALLMAGWMGAIGSLRGVAEAGIIASSLSGA
                     DERSEDMAKVQSFLENKIVVQQLIDYGVSSGEAKAKVEAMSARDLHRLASLTDRAAAG
                     ADDGLGLLIGLAILVILIIVILKLSHKEVVVR"
     gene            2939..3565
                     /locus_tag="VFK23_11105"
     CDS             2939..3565
                     /locus_tag="VFK23_11105"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319675.1"
                     /translation="MMPDSTEVNPSRRTFLKGAAGVVSGTVFAELAGLGVDLSALAAK
                     AGDVPIKRGKEVASICPYCAVGCGQIVMVDTATGRILDIQGNPDSPISQGTLCPKGAA
                     TYQLAVNPLRWTKVRYRAPQSDHWEEKPLDWAMERIAQLVKKTRDDNFNEFQEMPDAK
                     GGTVRKRVMNTYAIASLGGATMDNEWNYVHQKLMHALGVVYVENQARI"
     gene            3662..>4302
                     /locus_tag="VFK23_11110"
     CDS             3662..>4302
                     /locus_tag="VFK23_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HET7319676.1"
                     /translation="MGSNMAECHPVAFRWPLKAKTEHGAVLMHVDPRFTRTSALCDIH
                     APIRAGTDIVFLGAIINHVIHSERWNTDPFFREYVVNYTNAATLIAPDFKDTEDLDGL
                     YSGLSPDGKAYSNATWAYQREKPPAPATKESKTFTDLLLQKIPGRPKTDPTLRNPRTV
                     FRILERHYSRYTPVTVEKICGCPKEKFLQVAETLLNNSGRDRTSNIVYAVGWT"
BASE COUNT         1000 a         1225 c         1262 g          815 t
ORIGIN      
        1 aaccgcagcc gataaatgct gtgggccgcc atgatgacga gcatcaggcc ggcgtaatag
       61 gtctggtacc cgagctcccg gtgggacgcg atgtcgatca tggcgagaac gcctgcgccg
      121 tccgtgagga caacgaaaac catcgccggt tggagaatgc ggggaaagcg agggtggaag
      181 cttgaggcca gcactacggc gagagctgga atgacaaccg cgaaccggat gaaccagata
      241 gcgcgccaca taagcggagc cgcgtaaacg tccagaatgg cgaacaaacc gaagagaagg
      301 gcggtgagga cgagggcgta ccgggtgatc acgacggcgt tgcgggaatg ataacgccga
      361 aaagcagatt ccacatcggc gggaaagcga agccttactg ccgctcgagc cagatcgtcc
      421 cattcgcggc ggaaaagtgc caacttaccg attcccccca ttaaacgctt aaacgctgat
      481 ccccgataac aagaaggcat gcattctgcc tgagggggta attgcccagg ggatggaaat
      541 gaaaaagggg acgaattcga gattatgcat cactcctccc tagctttccg ggttggctct
      601 gcaattatct cgatgaaata caaatcgaag tggtaataag aatctacatt atcagcggga
      661 gtcggtcaag cgggagttgt cctcgcgagc tgtacccgca cgagccacaa atgagccact
      721 cgaacctaaa acacggaaat ggggaaccag gcgcgaggcc aaattccctt tgagtactgc
      781 gatggttccg tgtcaaagtg gatggggagt ataccttaga cttttaaaat aggccgcagg
      841 tcctcaagcc ggtcgaagag atgccatatg taattcccgg cacccctttc gcggtgggga
      901 tgcagatcgg ctatagcggg gtcgtcgaga taactctgaa aggcctcacg ggattcccat
      961 tccaccaaga tgagcacctg gcgcggagtg atatcaccct tctgcgcctc acgaaagcgg
     1021 cccagtgcca ccagtttgcc tccgtgagcc gccacctcgc gtgccgaacg ccttgaatat
     1081 gccatgtatt cttccctgtc gacgatatcg aacatgttca acgcataaac ggggccggta
     1141 gattccatgg cagactcctt ttcatatatt ttaaacctca ctggaattaa agcgtaatta
     1201 accagttact tccgatagag cgagaagtcg tagtggccta ttccccccgc ccggattaaa
     1261 gatttcctcc gtctttgccg aaagaacttc gagtagcagg aaaatataaa aaggaggatg
     1321 aggtgaaaac gagaatcttt acggttttgg cagttctcac tcttacggga tttgtcccga
     1381 tcgcagcccc agtgcatgcg ggaaccaacg tcaacgttaa cttggggatc ccagcccccg
     1441 tagtcgtcgc tccccagccg gtgtatgtgg agcaacctcc ggagatggtt gtcatccccc
     1501 ggtcgaacgt ctacttcgca ccgggtgtta gcgtggatct cttcttctat gacaaccgtt
     1561 ggtggaaccg ccggggggat cggtggtacc ggggcagcgg atacaacggg ccgtgggtgg
     1621 ccgtggggcc gcgacacgtc ccgggccccg tctaccgggt gcccgcgaac taccgcacgg
     1681 tctacggcca cgagaagccg atgccgtacg gccagtggaa gaaggcacac ggagaacacg
     1741 gcgggaaaca cagaggggag atgcacggca acaaacgcgg gcatcacgac gattagcgga
     1801 ggacgggtca acgggaatcg ggtactggta agttggctgg agaaaaaata tactcactga
     1861 cacgtaatgc ttataaacag taaatggagg gatcgatgat ggcatcattg cggaaaacgg
     1921 catggttcgt aatggtggcg ctcctcatgg cgggttggat gggagctatc ggctcgctga
     1981 ggggggtagc cgaggcgggc atcatcgcct cttccctttc gggggcggac gaacgatcgg
     2041 aggacatggc gaaggtccag tcgttccttg agaataagat cgtcgtccag cagctgatcg
     2101 actacggcgt gtcctccggg gaagcgaagg cgaaggtcga ggcgatgagc gctcgagatc
     2161 tgcaccggct ggcctccctg acggaccggg cagcggcggg ggcagacgac ggcctcggcc
     2221 tcctcatcgg actggcgatc ctcgtcatcc tcatcatcgt gatcctcaag ctgtcgcata
     2281 aggaagtcgt cgttcgctga cactgcagtt tggccggtta ccgggcgccg cccagaaacg
     2341 ggtggcgccc tttttattag gttctggaag ccacctctgc tccttgaccc tgctttatcc
     2401 cgatcttgtg aggaaaatgg ggacaaggtc aaacaacccc caatcttgat tgttacgacc
     2461 cacaccgcgg ttatatactt tacgtatact ctttcctcct gatgaggtgg aaaatgcgaa
     2521 gacctgtcgc cgccgtgatc ctgtttcttg tcctctcgtc gttcatcgtt ccaaacgcat
     2581 ggccagagcc aaactacggg aggaaagaat taaacaagct catcagcgga aaatccaaga
     2641 aggaagttcg cgcactcttg ggatcaccca actcccttgc agcatacgag gatgacgatg
     2701 gcggcctatg gagatatgga caaggcgacc ggagagattg ggggtttcgt tggacggttc
     2761 ttgacgagga cagcggagtc ccgtgttggg gtgtatctat taaattcaaa aatgggaatg
     2821 cggtatccgt ttccgtttac tcgtgcatgc aatgatccta taatttacaa acgataaacg
     2881 acaattcgtg gaacaacggc taccgatttg atccgttccc tcacggaaag gagaatcgat
     2941 gatgcctgat tccaccgaag tgaacccgtc tcgacgtacc ttcctcaaag gggctgccgg
     3001 tgtggtgtcc ggcactgttt tcgccgaact tgccggcctc ggcgtcgatc tgagcgcctt
     3061 ggcggccaaa gccggtgatg tgccgatcaa gagagggaag gaggtggcaa gcatctgccc
     3121 gtactgcgcc gtcggttgcg ggcagatcgt catggtagac acagccacgg ggcggatcct
     3181 cgacatccag gggaaccccg attccccgat cagccagggt accctctgcc cgaaaggggc
     3241 cgccacctat caactcgccg taaatcccct gcgctggacg aaggtgaggt accgggctcc
     3301 ccagagcgac cactgggaag aaaagcccct cgattgggcg atggagcgaa tcgcccagct
     3361 cgtcaagaag actcgcgacg acaactttaa cgagtttcag gagatgcccg acgccaaggg
     3421 cgggacggtc cgcaagcgcg tgatgaacac ctacgccatc gcatccctcg gcggggccac
     3481 gatggacaac gagtggaact atgtccacca gaagctcatg cacgccctgg gggtcgtgta
     3541 cgtcgaaaac caggcacgga tatgacactc ctccacggtc cccggtctgg gggtctcctt
     3601 cggtagaggc gggtccacca ccttccctcg cgacctcgtc cactccgacg cggttctgat
     3661 catgggctcc aatatggcgg aatgccatcc ggtggccttc cgctggccgc tcaaagccaa
     3721 aacggaacac ggcgccgtcc tgatgcacgt ggacccgcgc ttcacgcgca cgtccgccct
     3781 ttgcgacatc cacgccccca tccgggcggg aaccgacatc gtgttcctgg gggccatcat
     3841 caaccacgta atccacagcg agcgctggaa taccgaccct ttcttccggg agtacgtggt
     3901 caattacacc aacgcggcca cgttgatcgc ccccgatttc aaggacaccg aagacctgga
     3961 cgggctctac tccgggcttt cccccgacgg gaaggcgtac agcaacgcca cctgggccta
     4021 ccagcgcgaa aagccgccgg cgccggcgac gaaggagtcg aagaccttca ccgacctcct
     4081 gctgcagaaa atcccggggc gccccaagac ggacccgacg cttcggaatc cgcgtaccgt
     4141 ctttcggatc ctggaacggc actactcccg gtacacaccc gtgacggtgg aaaagatctg
     4201 cggctgcccg aaggagaaat tcctgcaggt ggccgagacc ctgctgaaca actccggccg
     4261 cgaccgtacg tcgaacatcg tctacgccgt ggggtggacg ca
//
LOCUS       DASSLD010000617         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_242458,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000617 DASSLD010000000
VERSION     DASSLD010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242458"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            374..979
                     /locus_tag="VFK23_11115"
     CDS             374..979
                     /locus_tag="VFK23_11115"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HET7319677.1"
                     /translation="MAFRKGTKVKKTVKKKASGKKKTAAKARKPAAKKTVKKAAKKPV
                     TKKAAKKASPPAAAVTEAVRPARISPAVQKRHEMLKKILLKKRNEVVQGLEAQMGRKL
                     LAETGQKIDSAMDSADQSALDVDQGIDYSLLEMKYEQYKDIADAFRKLQNNTFGLCEE
                     CGQEIDIKRLQVNPLARFCITCKQKKEEIERIQKEETRFKE"
     gene            1114..1189
                     /locus_tag="VFK23_11120"
     tRNA            1114..1189
                     /locus_tag="VFK23_11120"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1147..1149,aa:Met,seq:cat)
     gene            1275..>2012
                     /gene="tilS"
                     /locus_tag="VFK23_11125"
     CDS             1275..>2012
                     /gene="tilS"
                     /locus_tag="VFK23_11125"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET7319678.1"
                     /translation="MLEAGDRVLVSVSGGPDSVCLLSVLHELSKDMDLALHVAHLDHM
                     FRGGESAEEALFVADLARELGVPATVEQIDVPRYCRERGLSPQVGAREARYVFFSRVA
                     ADVGAGRIATGHTASDQAETFLMRLVRGAGVSGLSAIPPKRDTIIRPLIESTREDVLA
                     YLNARGLRFASDPSNTKPLYTRNRIRLEALPILKRFNPRIIETLAAEAALLRDEDEAV
                     EAHLAAVTDAAVMREEDAVSVKRDVFVT"
BASE COUNT          476 a          567 c          587 g          382 t
ORIGIN      
        1 gcagggaata gttcacggat gcagcgaatg agtaaaacga agtcgaagga atggcaacca
       61 gcaggatcag aaatgtgata tatattagaa gattacgacg gcgtgccttc atgcgtctcc
      121 tcatgccaac tcgatatagg gcttttattg tatcaaaaaa gcgttgaaat ggctactaga
      181 ggggcgcagg gaccggcggg tgcatcgtta cccattcgat ggacgcctcc ctgcggtggg
      241 gaccgcggac cggccggccg ggcacaatgg tataatttcc cgtttctctt acaatttgtc
      301 ttgaatttgc cttgagagat aactataatg ggtctccaat ctgccgacca gttctaggag
      361 agaggtgtag gatatggctt tcagaaaagg aacaaaagtg aaaaaaaccg tcaaaaagaa
      421 ggcctccggg aaaaagaaaa ccgccgccaa ggcccggaaa cctgcggcga aaaaaaccgt
      481 taaaaaggcc gctaaaaaac cggtcacaaa gaaggctgcc aaaaaggcca gccccccggc
      541 cgctgcagta acagaggcgg taaggccggc gaggatttcc ccggccgtgc agaagcgcca
      601 tgaaatgttg aagaagatcc tgctcaaaaa gcgcaacgaa gtcgtgcaag gcctggaggc
      661 ccagatgggc cgcaagctcc tggcggaaac aggccagaaa atcgactccg ccatggacag
      721 cgccgatcag tccgccctcg acgtggacca ggggatcgac tactccctgc tcgaaatgaa
      781 atacgagcaa tacaaggaca ttgccgacgc attccgcaag ctccagaaca ataccttcgg
      841 cctctgcgag gagtgcggcc aggaaatcga catcaagagg cttcaggtca accctctcgc
      901 ccgtttctgc atcacctgca agcagaaaaa ggaagaaata gagcgaattc agaaggaaga
      961 gacaaggttc aaggagtaag cgagggacct cgtttttccc gatagtttcc aggcccttcg
     1021 cttcgctcaa ggtgacggtt tctgggaaac cgtcagtttt tcttgacaaa aaggcgccga
     1081 tacagtatcg tatgcgtcta aaatttttca cggggcggtg tagctcagct ggtagagcag
     1141 gggaatcata atccctgtgt ccggggttcg agtccctgca ccgccaccat ttttcttccc
     1201 tttccgtact cataacgtat tcccccgctt ccatgctgct cacaaaggtc aaatcgacta
     1261 tcagacggtt tcagatgctt gaggccggag accgggtcct ggtctccgta tcgggagggc
     1321 ccgactcggt ctgcctcctc tccgtccttc atgaattgtc gaaggacatg gacctggcct
     1381 tgcacgtcgc ccatctcgac catatgttcc ggggcgggga atccgccgaa gaggcgctgt
     1441 tcgtggcgga cctcgcgagg gaactcggcg tccccgccac cgtggaacag atcgatgtcc
     1501 cgcggtattg ccgagaacgg gggctctcgc cccaggtggg agcgcgggag gcacggtatg
     1561 tgttcttctc ccgcgtcgcg gcggatgtgg gcgccgggcg gatcgccacc ggacacacgg
     1621 cctcggacca ggccgaaacg ttcctgatgc ggcttgtgcg gggcgcgggc gtgtcgggtc
     1681 tttcagccat cccgcccaag cgggacacta tcatccggcc gctgatcgaa tctacccggg
     1741 aggacgtcct cgcgtacctg aatgcccggg gtctccgttt tgcgtccgat ccatcgaaca
     1801 ccaagcccct gtacacgcgg aacagaatca ggctggaggc gctgccgatt ctgaagcggt
     1861 tcaacccccg catcatcgag accctcgccg ccgaggccgc cctgctgcgg gacgaggacg
     1921 aggcggtgga ggcccatctc gccgccgtga ctgatgctgc ggtcatgcgg gaagaagacg
     1981 ccgtctcggt aaagcgtgac gtcttcgtca ct
//
LOCUS       DASSLD010000618         2705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_339776,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000618 DASSLD010000000
VERSION     DASSLD010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339776"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1058
                     /locus_tag="VFK23_11130"
     CDS             123..1058
                     /locus_tag="VFK23_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7319679.1"
                     /translation="MALYILKRILLFIPTLLGITLITFVLMQSLPGDPAAGMAGERAS
                     PETIARIRAELGQDKPLALQYIGYLKLIASGELGRSYYTNRRILDDLLQKFPNTLRLA
                     LAAMLFASFFGVMLGVLSAAKRSTIWDRFIALISVGGISIPVFWLGLALMLVFAFYLR
                     WLPPSGMGDGSAVYLILPAVTLGTFSLSYIARVTRSSMLETLSQPYIAAARAKGLSEA
                     AVVLKHALRNSLIPVITLIGLDLGSYLNGAVLTETIFGWDGLGRYALDGIIKRDYPII
                     MGVVLFGAVVFVTMNLLVDISYHFLDPRVRIKSGE"
     gene            1212..2057
                     /locus_tag="VFK23_11135"
     CDS             1212..2057
                     /locus_tag="VFK23_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7319680.1"
                     /translation="MANIITLKDLKENIPALIAAIVVVLLVLVAVLGPALMSLASLDP
                     LAMDLDHLKQPPGKDHLFGTDSKGRDILARVVYGSRISLAVGVLASALSLAIGIFFGL
                     IAGYFGGRTDAVLTQFFDIFLAFPSLLLAIGISAVMPPGLASAMLAITLVGWAGFARL
                     VRGITLSLKEQTYIEASRALGAAPARILYHHILPNALPLILVAGSLRVGGFILLEAAL
                     SFLGLGVQPPTPTWGSMISLNRAYINSAPWMVVFPGLAISITVISFNILGDFLRDKLD
                     PRMQV"
     gene            2236..2670
                     /locus_tag="VFK23_11140"
     CDS             2236..2670
                     /locus_tag="VFK23_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319681.1"
                     /translation="MKVADMISEALEKKGFHNLKDAAKFLGISPELLRVSLNKRHIPK
                     DKTLVMIANKLGLDKSILIMTAHREKVPPEMQGLFLSPSEAKMNREKRIYPLSEEQCN
                     YLAKLMSTEEIQMVRKVRQVTDEAKTQIAGYVDFMYATKKIG"
BASE COUNT          512 a          874 c          737 g          582 t
ORIGIN      
        1 actggaagga gtgggaggcc ccacccacag ctggttcgag ccgtccggtg aatgacgtgg
       61 ccccggtctg tcaccttttt cccgttgcgg ttttccgcgg gattgtgcta cagtagcggg
      121 aaatggctct ctatatcctc aaaaggatac ttctcttcat ccccaccctc ctgggcatta
      181 cgctgatcac ctttgtcctg atgcagtccc ttcccggcga tcccgcggcg ggcatggccg
      241 gcgagcgcgc ttcaccggag accatcgctc gcattcgagc cgaactcggc caggacaaac
      301 cccttgcgct ccagtacatc ggctacctga aactcatcgc atcgggcgag ctcggccggt
      361 cctactatac caaccggagg atcttggacg atcttctcca gaagttcccc aacaccctcc
      421 ggctcgccct ggcagccatg ctctttgcct cttttttcgg cgtcatgctg ggcgttctgt
      481 ccgcggccaa aagaagcacg atctgggatc ggtttatcgc actgatctct gtcggcggca
      541 tttccatccc tgtcttctgg ctgggcctcg ccctcatgct cgtctttgcc ttctaccttc
      601 gctggctccc gccctcgggc atgggcgacg gcagcgccgt ctacctcatt ctgcccgccg
      661 tgacgctcgg caccttttcg ctgtcgtaca tcgcgcgcgt gacgcgttcg tccatgctgg
      721 agaccctttc gcagccctac atcgcggcgg cacgggccaa ggggctctcc gaagccgctg
      781 tcgtattgaa gcacgcgctc cgaaactcgc tcatccccgt gatcacactc atcggcctcg
      841 atctcggctc atacttaaac ggcgcggtcc tgaccgagac catcttcggc tgggacggcc
      901 tcggccgcta cgcgcttgac ggcatcatca aacgcgatta ccccatcatc atgggggtcg
      961 tgctcttcgg ggccgtggtc ttcgtgacca tgaacctgct cgttgacatc tcgtaccact
     1021 tcctcgatcc ccgcgtgagg atcaagagcg gggagtgaca gactgtggaa agagtctctt
     1081 agccgtcttt gcgagcgaag caatctcgct tttccataat tcaagggttg agagattgct
     1141 tcgtcgctga cactcctcgc aatgacggct aaacgaggct tgttcaaaaa gaggagaacg
     1201 gattcgtagc catggcgaac attatcaccc tcaaggactt gaaggaaaac atcccggcgc
     1261 tgattgccgc gatcgttgtt gttcttctcg tgctcgtcgc cgttctcgga ccggctctga
     1321 tgagtctcgc cagccttgac ccccttgcca tggacctgga ccacctgaag cagccgcccg
     1381 gcaaggacca tctcttcggc acggacagca aggggcggga catcctcgcc cgcgtggtgt
     1441 acgggtcgcg catctcgctt gccgtcggtg tcctggcctc cgccctgtct ctcgcgatcg
     1501 gcatcttttt cggcctgatc gccggctact tcggcggcag gacggacgcg gtcctgaccc
     1561 agttcttcga catcttcctt gcctttccca gcctgctgct cgccatcggc atcagcgcgg
     1621 tcatgccgcc gggcctggcc tcggccatgc tcgccatcac gctcgtcggc tgggcaggct
     1681 ttgcgcggct ggtgcgcggc atcacccttt cactcaagga gcagacctac atcgaggcct
     1741 cgcgcgccct cggcgccgcg cccgcccgga tcctatacca ccacatcctg ccgaacgccc
     1801 tgccgctcat cctggtggcc gggagcctcc gcgtcggcgg gttcatcctg ctcgaggcgg
     1861 ccctgtcctt tctgggcctc ggcgtccagc ctcccacgcc cacctggggc tcgatgatca
     1921 gcctgaatcg ggcctacatc aactccgctc cgtggatggt cgtcttcccc ggcctggcca
     1981 taagcatcac cgtgatcagt ttcaacatcc tgggagattt tctgcgcgac aagcttgatc
     2041 ccaggatgca ggtatgacgc gagcggcccc caacgggcag gagaatgggg actgcaagtc
     2101 cctggattca tacccacaaa aaatgttgta tttttttgtt cttatgctat agttgtacgg
     2161 taaggtctac agcggtagtc cgccgcaaaa atgtgcgggt atatgcaagt caaagaatta
     2221 aggaaggatc gtgacatgaa agtagccgat atgatcagtg aggcgctcga gaagaaggga
     2281 ttccacaacc tgaaggacgc cgcaaagttt ctcggcataa gcccggaact cctgcgggtc
     2341 tctctcaaca agcggcacat cccgaaagac aaaaccctcg tcatgatcgc gaacaagctg
     2401 ggcctggaca aatccatcct catcatgacg gcgcaccgcg agaaggtccc cccggagatg
     2461 cagggtctgt tcctctctcc atcggaagcg aagatgaacc gcgagaaacg catctatccc
     2521 ctctcggaag agcagtgcaa ctatctcgcg aagctcatga gcaccgaaga gatacaaatg
     2581 gtgcggaagg tccgtcaggt cacggacgag gccaagacgc aaattgccgg gtacgtcgac
     2641 ttcatgtatg caacgaaaaa gatcgggtga tcgtaagcgc ccgtatccgc gacatggatg
     2701 aaata
//
LOCUS       DASSLD010000619         4327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1514042, whole genome shotgun sequence.
ACCESSION   DASSLD010000619 DASSLD010000000
VERSION     DASSLD010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514042"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /gene="pssA"
                     /locus_tag="VFK23_11145"
     CDS             complement(<1..563)
                     /gene="pssA"
                     /locus_tag="VFK23_11145"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HET7319682.1"
                     /translation="MRKGIYILPNLITLSSMFAGFYSIVASFNGDFERAAWAILIASI
                     FDVLDGWVARVTHTATRFGIEMDSLADVISFGVAPGILAYTWTLSSFGKIGWLGSFFL
                     VACAALRLARFNVQMGSAEKKHFTGLPTPASALVIATTVLAYEEIVGILERLNLVRLA
                     DAVGKDYWVLALTFLLAGLMVSNITYH"
     gene            complement(560..1204)
                     /locus_tag="VFK23_11150"
     CDS             complement(560..1204)
                     /locus_tag="VFK23_11150"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018049211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HET7319683.1"
                     /translation="MMKNYGIPVAAEGWPFIVPLALATVLLFALGWRNAGAVLLVLTL
                     FVLFFFRDPERAVPQGEGVVVSPADGKVIVIKDIYEPDYLKQDVKQISIFLSVFNVHV
                     NRAPIGGAVEAVRYNPGKFHVASVDKASHLNEQTAMVIATGKNKVLVKQIAGLIARRI
                     VCYAKPGDTVKAGERYGLIRFGSRVDLFLPKDAELKVKLGDRIKGARDIIAVLR"
     gene            complement(1319..2335)
                     /gene="ilvC"
                     /locus_tag="VFK23_11155"
     CDS             complement(1319..2335)
                     /gene="ilvC"
                     /locus_tag="VFK23_11155"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET7319684.1"
                     /translation="MKVYYDKDADLNLLKGKKVAVVGYGSQGFAHSNNLKESGVDVMV
                     ALRKGSKSWEKAAGAGLKVVEPAEAAKAADIIMILVPDELQGGMYRKDIEQNIKKGAY
                     LAFAHGFNIHFGQIVPREDVNVFMAAPKSPGHLVRSQYTKGEGVPGLIAVHQDPSGNT
                     KDVALAYACAIGLGRAGVIETTFRDETETDLFGEQAVLCGGTTALIMAGYETLVEAGY
                     APEMAYFECLHELKLIVDLIYEGGISNMRYSISNTAQYGDLTRGPRLINEETKKEMKK
                     ILGEIQSGQFAKEWMLENMANKPTFNALTKKGQAHPIEEVGDRLRKMMPWLQKGKLVD
                     KSKN"
     gene            complement(2515..3135)
                     /locus_tag="VFK23_11160"
     CDS             complement(2515..3135)
                     /locus_tag="VFK23_11160"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajG family lipoprotein"
                     /protein_id="HET7319685.1"
                     /translation="MRITRLLFIALLAVSLHGCAEKGPILLAVSYQEPAEITPASSRL
                     TVGVSQFKDVRGKAASVLGKRTIPGGPENDFVVQGTVAGVATTSLKKALTARGFSVKD
                     VPWDLTAEGMKSDGSALLLGGEIKTLWLESTSDTLKTHLRATVQMRVVAGDPLEKKIV
                     RSVEVSSRLEQDLLYSREELEDALSEALTSAIDQIFRDEDLKKRLQ"
     gene            complement(3229..3702)
                     /gene="ilvN"
                     /locus_tag="VFK23_11165"
     CDS             complement(3229..3702)
                     /gene="ilvN"
                     /locus_tag="VFK23_11165"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET7319686.1"
                     /translation="MKHIISVLVENKFGVLSRVSGLFSGRGFNIETISIGPTLDPTIS
                     QMTIVTTGDERILEQIVKQLNKLIDVIKVTDLAEKEFVEREMCLIKVNAPTEHRAEAL
                     RIADIFRARVVDSSQKSYTFQVTGDEKKIVAFIELLRPIGIKEVVRTGKVAITRE"
     gene            complement(3746..>4327)
                     /locus_tag="VFK23_11170"
     CDS             complement(3746..>4327)
                     /locus_tag="VFK23_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7319687.1"
                     /translation="DAIIVTDVGQHQMWSAQFFKIDRPRTFLSSGGLGTMGYGFPAAI
                     GAKVAHPDKMVFSISGDGSIQMNIQELATSVENNIPVKVVILNNRYLGMVRQWQELFY
                     QERYSASDLGSTPDFVKVAEAYGALGLRAQKPSEVVPVLKEGIKAKKTVVMEFVIDRY
                     EKVFPMVPAGASIHEMIFGEEKKKEEKKLKAVK"
BASE COUNT          813 a         1337 c         1186 g          991 t
ORIGIN      
        1 ctgtgatacg tgatgttgct caccatcaat ccggccagga gaaaggtcaa tgccagcacc
       61 cagtagtctt ttccgaccgc atcggccagc cggaccagat tcaggcgctc aaggatgccg
      121 acgatctcct cgtaggccag aacggtggtc gcgatgacga gcgccgaggc cggcgtggga
      181 aggccggtaa agtgtttttt ctccgcgctc cccatctgga cgttgaaccg cgcaagccgc
      241 agcgcggcgc aggcgaccag gaaaaaggac cccagccacc cgatcttgcc gaacgaggac
      301 agcgtccacg tgtacgcgag aatgcccgga gcgacgccga aggatatcac gtcggccagc
      361 gaatccatct cgatgccgaa ccgcgtcgcc gtgtgcgtca cccgcgccac ccacccatcc
      421 agcacgtcga agatcgatgc gatcagaatg gcccatgcag cccgctcgaa atcgccattg
      481 aacgaggcca cgatggagta gaaccccgcg aacatgctgg acagggtgat aaggttcggc
      541 agtatgtaga tcccttttct catctgagca ccgcgatgat gtccctggcg cccttgatcc
      601 ggtcgccgag cttcaccttg agttccgcat ccttcggcag gaacagatcg acccgggagc
      661 cgaatcggat caggccgtac cgctctcccg ctttgaccgt gtcccccggt ttggcgtagc
      721 atacgatgcg ccgcgcaatg agccccgcga tctgcttcac aagcaccttg ttttttcccg
      781 tcgcgatgac cattgcggtt tgttcgttca ggtgtgacgc cttgtccacc gatgcgacgt
      841 ggaattttcc ggggttgtac ctgaccgctt cgaccgcgcc tccgatgggc gcccggttta
      901 catgcacgtt gaagacggac aggaagatgc tgatctgctt cacgtcctgc ttcaggtaat
      961 cgggttcata gatgtccttg atcacgatta ccttgccgtc ggcaggtgaa accacgacgc
     1021 cctcgccttg gggaacggcg cgctccgggt cccggaagaa gaacaggacg aacagcgtaa
     1081 gcacgagcag gaccgcgccc gcgttcctcc agccgagcgc gaacaagagc accgtagcca
     1141 gcgccagagg gacgatgaac ggccatcctt cggctgccac ggggattccg tagtttttca
     1201 tcatgagata aaaccttcaa ccacagagga cacggagagc acagagaagg atcagattct
     1261 ggatttgaag tctgctaaga atttctcagc gtcctctgtg tcctctgtgg ctaatttcct
     1321 agtttttgct cttgtccacc agcttgccct tttggagcca gggcatcatc tttcgcaaac
     1381 ggtctcccac ttcctcgatg ggatgtgcct gccctttttt cgtgagcgca ttgaacgtcg
     1441 gcttgttcgc catgttctca agcatccatt ccttcgcgaa ctggccggac tggatctcgc
     1501 cgaggatctt tttcatctct ttctttgtct cctcgttgat caggcgcggg ccgcgggtca
     1561 ggtctccgta ctgggccgta ttgctgatcg agtaccgcat gttcgagatg cccccttcgt
     1621 agatcaggtc cacgatgagt ttgagctcgt gcaggcactc gaaataggcc atctccgggg
     1681 catagccggc ttcgacgagc gtctcgtacc cggccatgat gagcgccgtc gtgccgccgc
     1741 aaagcaccgc ctgttcgccg aagaggtccg tctcggtctc gtcgcggaag gtggtctcga
     1801 tgacgcccgc ccgtcccagg ccgatggcgc aggcgtatgc cagggccacg tccttcgtgt
     1861 ttccggaggg gtcctgatga acagcgatca acccgggcac gccctcgcct ttcgtatact
     1921 gggagcgcac caggtggccg ggactcttgg gggccgccat aaacacgttc acgtcttcgc
     1981 gcggcacgat ctggccgaaa tggatgttga acccgtgggc gaaggcgaga taggccccct
     2041 tcttgatgtt ctgctcgatg tccttccggt acatcccgcc ctggagttcg tcggggacca
     2101 ggatcatgat gatatccgcg gccttggcgg cttcggccgg ttcgacgacc ttgagtccgg
     2161 cgccggccgc tttttcccag gacttgctcc ccttgcggag cgcgaccatg acgtccacgc
     2221 cgctttcctt gagattgttc gaatgggcga agccctggct gccgtagcct accacggcaa
     2281 ctttcttgcc tttgaggaga ttcaggtccg cgtctttgtc gtaataaact ttcatcgtgt
     2341 cccctttcaa cgataagata atgtcgaaaa ttgccgaatc cgccattccg gcacggtccg
     2401 gccggaatcc agtgtcttta tctttttgtt atcatgaaaa acctggattc acgataaagc
     2461 ctgccccggc atgcttagaa ccggggggaa tgacgagaag gtgttgttgt agtcttactg
     2521 gaggcgtttc ttcaggtctt cgtccctgaa tatctgatcg atggccgagg tcagggcttc
     2581 ggagagcgcg tcttccagtt cctcgcggga atacagcaaa tcctgctcaa gcctgctgct
     2641 cacctcaacc gacctcacaa tctttttttc caacgggtcg ccggcgacga cccgcatctg
     2701 gaccgttgcc cgcaggtggg ttttcagcgt atccgaggtg gattccagcc acagcgtttt
     2761 gatctcgcct ccgagcagca gagctgaacc gtctgacttc atgccttcgg cggtcaagtc
     2821 ccagggaacg tccttcacgg aaaaaccgcg cgctgtaagc gctttcttca ggctggtggt
     2881 cgcaaccccc gccaccgtgc cctggaccac aaagtcattc tcgggcccgc ccgggatcgt
     2941 cctcttgccc aaaactgacg cggccttgcc gcggacatcc ttgaactggc tcactcccac
     3001 ggtcagcctc gaagatgccg gcgttatttc tgcgggttcc tgatacgaga cggcgagaag
     3061 gatcggcccc ttctcggcac atccgtggag cgacacggcc agcaaagcaa tgaacaacag
     3121 tctcgttatc ctcatgatta tccttcccgt tgcgtctcgt tcttgattca ggatgcgcga
     3181 tccacgattg acaacctgaa tcctgcatcc ggcatcgtgc atccagactc attcccttgt
     3241 tatcgccacc ttgcctgttc gcacgacttc cttgattccg atcggccgga ggagttcgat
     3301 gaaggccacg atcttcttct catccccggt cacctggaaa gtgtagcttt tttgcgagga
     3361 atcgacaacg cgggcacgga agatgtccgc gatcctgagg gcctcggccc ggtgctccgt
     3421 cggcgcgttt accttgatga gacacatctc gcgctcgacg aactcctttt ccgcaaggtc
     3481 cgtcaccttg attacatcga tgagtttgtt gagctgtttg acgatctgct caaggatgcg
     3541 ttcgtcgccc gtggtcacga tcgtcatttg cgagatcgtc gggtccagcg ttggcccgat
     3601 ggagatggtc tcgatgttga agcccctgcc ggaaaacagc cccgacacgc gcgaaagcac
     3661 gccgaattta ttttctacga gcaccgatat gatatgcttc atcgtctctc cgctttcagg
     3721 ttctactctg aactccgaac tatttctact tcaccgcttt gagtttcttc tcttcctttt
     3781 tcttctcttc cccgaagatc atctcgtgga tgctcgcccc ggcgggcacc atggggaaaa
     3841 ctttttcgta ccggtcgatc acaaattcca tgaccaccgt tttcttcgcc ttgataccct
     3901 ccttgaggac ggggacgacc tccgagggtt tctgcgcgcg aagcccgagg gcgccgtagg
     3961 cctccgccac cttgacgaag tccggggtgc tgccgagatc ggacgccgaa taccgctcct
     4021 ggtagaacag ctcctgccat tgccgcacca tgccgaggta ccggttgttc aggatcacga
     4081 ccttcaccgg gatattattc tccacggacg ttgcaagctc ctggatgttc atctggatgc
     4141 tgccgtctcc ggagatgctg aaaaccatct tgtccggatg ggcgaccttc gccccgatgg
     4201 ccgccgggaa cccgtagccc atcgttccca gtccgcccga ggaaagaaag gtcctcggcc
     4261 tgtcgatctt gaagaattgg gctgaccaca tctggtgctg gcccacgtcg gtcacgatga
     4321 tggcgtc
//
LOCUS       DASSLD010000620         2632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_111645,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000620 DASSLD010000000
VERSION     DASSLD010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111645"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFK23_11175"
     CDS             <1..185
                     /locus_tag="VFK23_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7319688.1"
                     /translation="YILEGAGVMHINGESSPVRPGSAVYIPPRARQCITNPGPADLKF
                     ICIVDPAWRKEDEEVL"
     gene            583..2448
                     /locus_tag="VFK23_11180"
     CDS             583..2448
                     /locus_tag="VFK23_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7319689.1"
                     /translation="MLQVTNIEKSYGKQVLFDGVSFTVNPRERVGLVGRNGYGKSTLF
                     RMILGEEHQDAGTITVPSGYTIGHLSQHIHFTEDTVIGEACLALPKNEDHIDETYKAE
                     AVLHGLGFTNADFGRHPSSLSGGYQVRLNLAKVLVSECNLLLLDEPTNYLDIVSVRWL
                     TQFLRAWRNELIIITHDRDFMDSVTTHTMIIHRRKMRKVAGGTQKLYDQILLEEEIYE
                     KTRQNDEKKRKEVEQFINRFRAQATKASAVQSRIKALARHEKLDKLEEIKTLDFKFRS
                     APFEAKWLLTVQDLSFSFSPDGPGLIDGLSFAVGKKDRIAVIGKNGKGKTTLLNLLAR
                     ELSPTNGEIKTHPNTRIAYFGQTNINRLRPEKTALQEIMDVHPDNAQGESRAICGLMM
                     FEGDNALKKVSVLSGGEKSRVLLGKLLVSPSNLLMLDEPTNHLDMESIDSLIEAIDDF
                     DGAVVIVTHSELILEAVATRLIVFDNDAVSVFEGTYFDFLERVGWSDEGGVRVKKGKK
                     QEAGGNANRKDAKKLRSAIVSDRSRALSSLKARIAALEEEIMKLEQAVERDTQELLDA
                     SVKGRALAIRQLSQSVSEGKARIEALFSELEKATAEHDAKAREFDRQMNELAQIP"
     gene            complement(2509..2625)
                     /locus_tag="VFK23_11185"
     CDS             complement(2509..2625)
                     /locus_tag="VFK23_11185"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319690.1"
                     /translation="MLLSALDPGGSPQLHQAAGIIPATFINYFLNSYWTFKE"
BASE COUNT          597 a          792 c          792 g          451 t
ORIGIN      
        1 actatatcct cgaaggcgca ggcgtcatgc atatcaacgg ggaatcttcg cccgtccgtc
       61 ccggctcagc cgtgtacatc ccgccccggg cgagacagtg catcacgaat ccggggccgg
      121 cggacctgaa atttatctgc attgtcgacc ccgcgtggcg aaaagaggac gaagaagtcc
      181 tgtgatgcgg aatcgggcgc cgtttcggcg catcgtggcc ggactccgag gaatctattg
      241 cttttttccc gacggcgatt taccccacgg gcgtgaagcg tgattatatc gaaaataatc
      301 tccggtcatc tccgaaaaaa tcttgccatt acaccccgcc tgcgttatta tgcgtagcta
      361 ttgttaagct ggaactgtcc aggtctacct ctaagacaac tgcaaaggcg atatcgaatc
      421 tagccgtcat tcccgaatga ttctatcccc gatagagtca ttcgaggacg aatccatttt
      481 gagttcggtc cttagaatcg ccaatggatt cccgctggag cctgccccag catgattaaa
      541 gccggggcgg gaatgacaaa cgagagacgc caaggttagc taatgctcca ggtcacgaat
      601 atcgaaaagt cctacggcaa acaggtgctg ttcgacggcg tcagcttcac ggtgaacccg
      661 cgagagcgcg tgggtctcgt cggccggaac ggctacggca agtccacgct cttccgcatg
      721 atcctgggcg aagagcacca ggacgccggg acgatcacgg tgccttcggg atacacgatc
      781 ggccacctat cccagcacat ccatttcacc gaggacacgg tgatcgggga ggcgtgcctc
      841 gccctcccga agaacgaaga ccatatcgac gagacctaca aggccgaggc cgtcctgcac
      901 gggctggggt tcacgaacgc cgacttcggc cgccatccct cgtccctgtc cggcgggtac
      961 caggtgcggc tgaacctcgc caaagtgctc gtctcggagt gcaaccttct tcttctggac
     1021 gagcccacga actacctcga catcgtctcg gtccgctggc tcacccagtt tctgcgcgcc
     1081 tggaggaacg agctcatcat catcacccac gaccgcgact tcatggacag cgtcacgacc
     1141 cataccatga tcatccaccg ccgcaagatg cggaaggtgg cgggcggcac gcagaaactg
     1201 tacgaccaga tcctgctgga agaggagatc tacgaaaaga ctcggcagaa cgatgaaaag
     1261 aagcgcaagg aggtcgaaca gttcatcaac cggttccggg cccaggccac caaggccagt
     1321 gcggtccagt cgcgcatcaa ggcgctcgcc cggcacgaga agctcgacaa gctcgaggag
     1381 atcaagaccc tcgatttcaa gttccggtcc gcgcccttcg aggccaagtg gctgctgacc
     1441 gtgcaggacc tgtccttttc cttctctccg gacgggcccg ggctgattga cggcctctcc
     1501 ttcgccgtgg gcaagaaaga ccgcatcgcc gtgatcggaa agaacggcaa gggaaagacc
     1561 acgcttttaa accttctggc gcgggagctg tcgccgacga acggcgagat caagacccat
     1621 ccgaacacga ggatcgccta cttcggccag acgaacatca accgcctgag accggaaaag
     1681 acggcgctcc aggagatcat ggacgttcac cccgataatg cccagggaga gtcgcgcgcg
     1741 atctgcgggc tcatgatgtt cgagggcgac aacgcgctga aaaaagtgag tgtgctgtcg
     1801 ggaggcgaga agagccgcgt gcttctgggc aagctcctcg tctccccctc gaacctgctg
     1861 atgctggacg agccgacgaa ccacctcgac atggagtcca tcgactccct gatcgaggcg
     1921 attgatgatt tcgacggcgc ggtcgtcatc gtgacgcaca gcgaactgat cctcgaagcg
     1981 gtggccacca ggctgatcgt cttcgacaac gacgccgtgt cggtcttcga ggggacgtac
     2041 ttcgacttcc tcgaacgcgt gggctggagc gacgaggggg gcgtgcgcgt gaaaaaaggg
     2101 aagaagcagg aagccggcgg gaacgcgaac aggaaggacg cgaagaagct tcgctccgcc
     2161 attgtgtccg accggtcccg ggccctgagc tcgctcaagg cccggattgc cgccctcgaa
     2221 gaggaaatca tgaaactcga acaggccgtc gagcgcgaca cgcaggagct gctcgatgcg
     2281 tcggtcaagg gcagggcgct cgccatcagg cagctgtccc agtccgtgag cgagggcaag
     2341 gccaggatcg aggcgctctt ttccgagctg gagaaggcaa cggccgagca cgacgcaaag
     2401 gcgcgggagt tcgaccggca gatgaatgaa ctggcgcaga taccctgagc aggacgcagg
     2461 gaagttccga aacatccctc cccccgcgaa cggcggtccg catcccccct actctttgaa
     2521 cgtccagtag gaattcagga aataattgat gaacgtggcg ggaataatgc ccgcggcctg
     2581 atgaagctgc ggcgacccgc cggggtcgag ggcggagagg agcaggaagg tc
//
LOCUS       DASSLD010000621         3285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_144766,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000621 DASSLD010000000
VERSION     DASSLD010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144766"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFK23_11190"
     CDS             complement(<1..200)
                     /locus_tag="VFK23_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HET7319691.1"
                     /translation="MTGYALKRTPTHPGEILKQDFLVPLGLTQVQLAKTLKTSFRTID
                     VILNEKRSVSPDMALRLARYFGT"
     gene            508..1227
                     /locus_tag="VFK23_11195"
     CDS             508..1227
                     /locus_tag="VFK23_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET7319692.1"
                     /translation="MKFVRDPQSIYRFETPLVNGKFSGRWHFIFGDNENYDPLYKDFG
                     TLCAFNDNTFTPGAVWPLHWHHGHEMVTYIVDGEFRHEDEHGKGSVLRKGWVQHTTAG
                     SGLWHAKINNRSDLPLRFIQMLFYPALDHLNPSVEQKAFKLTDMTGCFLPLVSNRDKG
                     TLPIASDARVYSGFFRPGTATCYNLEPGRGIYLYVLKGGPVFVNGAGVPAFGAAMITE
                     EKRIDVIPVQDAELLLVDVKL"
     gene            complement(2429..2635)
                     /locus_tag="VFK23_11200"
     CDS             complement(2429..2635)
                     /locus_tag="VFK23_11200"
                     /inference="COORDINATES: protein motif:HMM:NF018630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HET7319693.1"
                     /translation="MKRTSNEDHGEVRSPCVKKCSLDRNKICPECYRSIDEIVSWRDA
                     DNSMKRTILEAAKLRRLKAVARGR"
     gene            complement(2783..>3285)
                     /locus_tag="VFK23_11205"
     CDS             complement(2783..>3285)
                     /locus_tag="VFK23_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319694.1"
                     /translation="SFEYKNAFLYVVIREEPGLGPDSYLYCCGIHLSRFCRLAWRKGG
                     ICSNPAVRGIQLLRIHVSAFAAKQGMIVAEAKGFSSLPIVPPGNGWYHQDPLKLERTT
                     PERIRELLEFSVMDLVKTVLAVCVPEARIPAALPGAAELQKYLESLSVAEERARVMAY
                     EKENPA"
BASE COUNT          744 a          896 c          823 g          822 t
ORIGIN      
        1 gtcccgaaat aacgggcaag gcgcagcgcc atgtcagggc tgacactccg tttctcattc
       61 aggatcacat cgatagtcct gaacgatgtc ttgagcgtct tagcaagctg cacttgggta
      121 agcccgagcg gtacaagaaa gtcctgcttc agaatctctc cgggatgggt aggagtcctc
      181 ttgagtgcat atccggtcat agcatttttc tcctcagtga taatcggtga taatcggtcg
      241 cttctacccg cgcttgtagg caatcttgta cgaccggcag tagaatcagc atcccctttt
      301 gttcggggaa aaggcaagtg tcacttctga cttctaactt cttacttcta acttctaact
      361 tctgaccggg tttctgctac ttaagtcgct aatccgaaaa taatacttct gtaaccttgt
      421 ctccacccac tcgtcaaata tatgaaaaga gcaagttaga gagcgaagcc cctccggcaa
      481 caccgggact tacggggagg taatcctatg aaattcgttc gcgacccaca gagcatttat
      541 cggttcgaga ctccgcttgt gaacggaaaa ttcagcggaa gatggcattt cattttcggc
      601 gataacgaga actatgatcc gctctacaag gattttggaa cgctgtgtgc cttcaacgac
      661 aatacgttta cccccggcgc tgtatggccg ctgcattggc accatggcca cgaaatggtc
      721 acctacatag tcgatggcga gttcaggcac gaggatgagc acgggaaagg ctccgtactc
      781 aggaaaggat gggtgcagca cacgaccgca ggcagcggcc tgtggcacgc aaagatcaac
      841 aaccgtagcg accttcccct gcggttcatc cagatgttgt tctatcccgc cctggaccac
      901 ctcaacccct ccgtggaaca gaaggccttc aagctgacgg acatgaccgg ttgcttcctg
      961 ccgctcgtgt cgaaccggga taaaggcacc cttccgatcg cttcagacgc ccgcgtctat
     1021 tccggttttt tccggcccgg aacggcgaca tgctacaact tggagcccgg acgcggcatt
     1081 tatctttacg tgctgaaggg gggaccggtt tttgtaaacg gagcgggtgt cccggcattc
     1141 ggggcggcca tgatcaccga ggagaagaga attgacgtga tccccgtaca ggatgcggag
     1201 cttttgctcg ttgatgtgaa attatgataa agtcgaatcc cgcttctccc tctgcgtcct
     1261 cttggtgata atcgcctttt tccgcaatcc gaaattgttg agacgttcga aagaagaaaa
     1321 aacccggcgg aagggaaacc cctcccgccg gggcatcgta tcatattgaa tcagccgcag
     1381 gaccggtcct atgatcctat accccgtccc gcggatgcgt tgcgcttaga ataacctcag
     1441 gacaaagtgt ccgatacccg ctgcgccagc aagtaccaga acagcgatga tcccgtattt
     1501 ttttgcctgt tcagacatgg ttttcttcct ccttccgttt agtaggcagg agaagtgcgc
     1561 attgtatgaa gaaactccgg tccgacttcc ctgccccgat gaaaaagccg gcttcccgaa
     1621 gtttgttact tccggcaatc cggctgaaaa gaggtcgatc ttccggcttt ccagagaagg
     1681 ataagcctga tcaggacctc gagtctgttt ccttatgtgc ttcccgcgat tttttttccg
     1741 ttaagaaatt cgtttatttt ttgttgcggc ggccgtcatg atgattcagc aagtaccttg
     1801 gaatcctcct gatgaggact gccgcaccta ttcgtacgag ccctgacggg agggagactc
     1861 cctcccgcca gggaatcagt gtcagtattt gaatcatcct caggaccggt ttgatgattc
     1921 tataccggcc cttcggatgt gtgcgcttag atgactttga ggacaaagtg tccgataccc
     1981 cacgcagcag caagcgcgac ggctgtaatg gccccatatt tctttgtctt atatgacatc
     2041 gtttcctgtc ctccttcgcg taaaattcgg caggagacaa tacgctgagg aaaactccgc
     2101 gaggcccctg ccccaatgaa aaagccggca ttcccgaaga tcatttcctt cggcaacccg
     2161 gcttaaaaga ggtcgatctt ggatcgcgac ctcgaatttg cttcactatg tgttactgat
     2221 taaaatatat ctcgcgatgt ttgagtttgt caagaaattt gtttattttt ttttatgcgg
     2281 cggcctttat tatgcggcag cgaatacttt agaaagaaaa agggcggcag gttcaggctg
     2341 tatttctact gccaaacagg caagcatgtc acaccgcaag aatggaagaa tataaacatt
     2401 atagacctgt ccctgagcca ttccctggct acctgcccct ggctaccgcc ttaagacgcc
     2461 tgagtttcgc ggcttcaagg attgttcgct tcatggaatt gtcggcatcg cgccatgaaa
     2521 cgatctcatc aatgctgcga tagcactccg gacagatctt gttccggtcg agcgaacact
     2581 tttttacgca gggtgatctc acctcaccat ggtcttcatt acttgttctc ttcatagttt
     2641 cgcatgggtt cggaggttcc gctttttgta ttttccttgc tgcttgctgc ctgcccctgg
     2701 ttacgcctta tgatcttgct ccctctgtgt cctctgtggt gaaaatcgct tttgtccgaa
     2761 ttccgcattc tacattccac agttacgcag ggttctcctt ttcataggcc atcacccgcg
     2821 cacgttcctc cgccaccgac agcgactcaa ggtacttctg caactcggcc gcacccggta
     2881 aggcagccgg tatccgggcc tcagggacgc agacggccag gaccgtcttc acaagatcca
     2941 tcacgctgaa ttcgaggagt tcgcggatac gctcaggcgt ggtccgttcg agcttgagcg
     3001 gatcctgatg ataccatcca ttgccgggag ggacgatggg gagtgaagag aaacctttcg
     3061 cctctgccac gatcatgccc tgctttgccg caaaagcgga aacatggata cgcagaagct
     3121 ggatcccccg caccgcagga ttggagcaga tgccgccctt tctccaggcg agacggcaga
     3181 accgggaaag atggatcccg cagcagtaga ggtagctgtc cgggccgagt cccggttcct
     3241 cccggatcac gacatacagg aaagcgtttt tatactcgaa cgaac
//
LOCUS       DASSLD010000622         9709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_275105,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000622 DASSLD010000000
VERSION     DASSLD010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 9709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9709
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275105"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(459..947)
                     /locus_tag="VFK23_11210"
     CDS             complement(459..947)
                     /locus_tag="VFK23_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET7319695.1"
                     /translation="MIATLRKLGGIGVLILSTILIAGTASGELLTKKALSLEDAKKVA
                     AGAAAEARKNKWNMDIAIVDDGGHLIYFERTDGTLLASADLAIGKGRTAVVYQRPTKA
                     LEDALNGGQYGILSFPNAVIREGGLPIVVDGKIVGGIGVSGGKPSEDAQVAKAGVDAL
                     TK"
     gene            complement(961..1251)
                     /locus_tag="VFK23_11215"
     CDS             complement(961..1251)
                     /locus_tag="VFK23_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319696.1"
                     /translation="MNIDNVLILKEGQAVGRKTILMLAVGAFVGALAGGAMADILGDS
                     PQSDQVLLTSTQEFVPEGNWAGTDWQTSGPVEIVAIPVMVSEESWMKDYGND"
     gene            complement(1262..1672)
                     /locus_tag="VFK23_11220"
     CDS             complement(1262..1672)
                     /locus_tag="VFK23_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319697.1"
                     /translation="MKRTFVALVVGFIFLVPVIGWSAQPGGTLPEQVASLEKQVASLA
                     AQVAKLQDQVANLTNQFNKEVATRQAELVPIHPRMGMAFNELYGQVAQAGCGWLELES
                     KKGDVTEYHVALSRPGCNTKIHYHFYNDELVKIE"
     gene            complement(2008..3321)
                     /locus_tag="VFK23_11225"
     CDS             complement(2008..3321)
                     /locus_tag="VFK23_11225"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HET7319698.1"
                     /translation="MNIFGRVPGFSWPRLGKQILVSVLSWSLLFATWPQKMSAQDAQP
                     PAPDGQAPPYTQQSPEQLQRLVAPIALYPDSLVAQILEASTFPEQVVEADRWVQAHPD
                     LEGEALGQGLDEQPWDPSVKAVAAFPSVLGNMDKNLSWTSSLGDAYYNQQQDVMDAVQ
                     VMRQRAQESGNLTTTPQQTVTTQGSTIIIEPASPDVVYVPAYDPWLIYGYPIVAWPGW
                     YPYPGIWFGGPYLSFGIGFGIGWFGGFGWGWDHWGCDWHNRYAIYDHDRYYSRSRTFY
                     NRNTFYRGGGGRGGVYNRYGTASRTFNGNRQAARGYAEPRGQSSVRSGAYTPGAAARS
                     FNGNRQTARGYAEPRGQSSVRSGAYTPGTEARSFNGNRQAARGYAEPRRQSSVRSGAF
                     SGYGQGGQTRSFSSRGSASIVGGGARGSGEARGGGGTRGGGGERR"
     gene            complement(3602..4717)
                     /locus_tag="VFK23_11230"
     CDS             complement(3602..4717)
                     /locus_tag="VFK23_11230"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7319699.1"
                     /translation="MRRLLAVIERDLIKFKRNPVVIAMSILMPIIYLVILGNSFQGKL
                     TDLPVAVVNQDSGPYAKRVMENLRAIQAGPKTFRITNLLSEGEAVNGVKEGIYKAAVI
                     IPVDFSKKMSLQARPEVGLFLDNPDNISSETIRNLLSAALFSIRSEYVPIREKTGEVL
                     LRDINLYPKVDYYQSLVPGVIIMAIFLGTLTSGVFNLVMDRFLGIEESYLLTPLTKTH
                     IVSGLVISGLSITTAIAAIITVTSMLLTGIPLAIGLDQFFPLFSVVVLTTLCLLSLMF
                     VILGRARHPRIVGIISGFLNVILFFPSGAVYPIESFPGWLKAFAKCNPEVYAVHALKS
                     ILFKNAGITSISGDILFLSLFTIVLMTIAILTFKRTL"
     gene            complement(4714..5721)
                     /locus_tag="VFK23_11235"
     CDS             complement(4714..5721)
                     /locus_tag="VFK23_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012185430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7319700.1"
                     /translation="MQKGKAIVVSDITKEYGSIVAVDHVSFEVDEGEFFGFLGPNGAG
                     KTTLIRMLTTLLRPTNGKAFVSGCDVSEEPAEVRKRIGVVPQAMTSDLDLTGHENMDL
                     YGRFYGIRGRERKERIRYLLEMLGLTQRANDLVATYSGGMRRRLEIARVLVHRPTLLF
                     LDEPTIGLDPQSRRVVWDFLKKLIEGDSMTIFLTTHYMEEAETLCGRVAIIDAGKIIA
                     MGTPSALKADIPGNDIVSLLVGDLSPELVRAIGELPFVHKAIAEENSLRVYVDNGSKN
                     LPVLIDDVRQAGGTILSASVHEQSLEDVFIHHTGKSIREEEVKKVSFLVGAGVPQSFG
                     R"
     gene            complement(5742..6791)
                     /locus_tag="VFK23_11240"
     CDS             complement(5742..6791)
                     /locus_tag="VFK23_11240"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7319701.1"
                     /translation="MKKWTKRVIALVVVFVVVLLAICVSLLLRNRKPPVFHVTGIIDG
                     VEVNLSSKVAGKISRISCNEGDKVQAGQVVITLESDDIKASVEQAMAGVERAKAGVKV
                     AEASIENGRANILAAEADVKSAAADLEKAGAQMEESRRQADRRDDLYKKNLIAKESRD
                     QMVTEYAVNVAAYDSSKEKLNAARSRKDVAASQLNAAISQWNSSKAMLKEAEANRAFF
                     RSKLDDTSIVTPITGTVIFKAIETGEMVSPGMAVLTIVDLNSLYARVDIDETKIGRVV
                     LNGKAFIRVEGVPVKTFEGRISEIGRSGEFATQRDVVRGREDIKTFRVKVRVDDPTGI
                     LKPGMTVDVEIPGKT"
     gene            complement(6836..7282)
                     /locus_tag="VFK23_11245"
     CDS             complement(6836..7282)
                     /locus_tag="VFK23_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7319702.1"
                     /translation="MYGKILVPIDGSETSKRGFTEACRLAKEQGARLRCLFVVDEHYL
                     TANYMGFMYLPDLIDKLRDNGKEILDEAAEQARKSGVQVESVMRESAERRVSFLILDE
                     AKTWPADLIVMGTHGRRGLSHLALGSDAESVVRNSAVPVLLVRSGV"
     gene            complement(7422..8336)
                     /locus_tag="VFK23_11250"
     CDS             complement(7422..8336)
                     /locus_tag="VFK23_11250"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HET7319703.1"
                     /translation="MNTRTTIKVSDSSKLHNRFIRKEIRRRRPGRMDLCVQNVPPVFP
                     PCLGGIQISEKGKRMAGSGSTKATVYNKAGVGVRKMEATRWLAALILLTVMFAGIYLY
                     SKNSKTVATFGLPVVLVVACGFIYWVKAMGNKAEVYSKRALDARRGAAAEEDVGNLLG
                     ELPAGYFVVNDFVLRRGNIDHIVISTKGIFTIETKSHRGVVSCEGEILKRDGKPFEKD
                     FITQGWREALSLRDLLTSQGISAPKPQPVLLFANADVRVRKQVRGVEIISRWHFPLYL
                     ERLQKRITAKEAEKIFEILKLSQSQMFV"
     gene            complement(8384..>9709)
                     /locus_tag="VFK23_11255"
     CDS             complement(8384..>9709)
                     /locus_tag="VFK23_11255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7319704.1"
                     /translation="LLIYTLLLFRFDRKREIYRLSFLFDLAFVYLLIVNSGGFASSFF
                     IGFYLLTALHAFYYGYRSGLVVAAMCTVIYLFAGLRISAIEPIDFLLRISFLFLIALP
                     IGLLSAILRRDKEKIESLNRNLAESLETLRHLQGKLVEAEKLSALGRLTADVAHEIRN
                     PLTAIGGFAKRLEKRLPENTAEKEYAKIVVKEVARLERILRDTLTFSREAKYHLQSAD
                     MNHLLAQEEAKFDGICREKHLLFAVRPAPGLPPCIVDEDQIRQALDNLLTNAIDAMTD
                     GGTLTLATRTACENGTHYVVIDVSDTGPGISPDLVKRIFEPFYSTKEIGQGTGLGLSI
                     CKKIMEEHRGSIRVSTAPGGGAMFSLFLPYLPVDEIFKAQCWEIMRCGVEKSENATER
                     CPAFPNYGRICWSVAGTLSETKVYCPVAEKIGDCHKCTFYEMVKPFCPT"
BASE COUNT         2130 a         2821 c         2466 g         2292 t
ORIGIN      
        1 tcagcggatt ctgatcggag gattccatta ccgggggcaa cgaaaaaagg gaacttggct
       61 tcgcgccgag ttcccttttg gttttgcgat ggagctcctc atcaaagaac tgcccaggat
      121 ttctgagagg agaaaccttg agcggagatc tactcttcac ctcggtcgac atggggggcg
      181 ctcccctcct tcaaaggaca ggggaccttc ggggggctgg cctgtaggtc ccatctcacc
      241 ttgattcttt aggctcagac atagccccag ggtcacgatg atctgccccc cggttttaga
      301 tcaactgatt gaggggattt tccccggagg tcggatggac tgcaaacccc gcgcaaacaa
      361 caggcatttt gggggtggaa aggggggttc cccggggagg gtgtcgatgt gggaaacccc
      421 cttgggccgt tggaaaggtc ttgtggaaac aggccggatt acttcgtcag ggcgtcaact
      481 ccggccttgg caacctgggc atcttctgaa ggcttgcccc cgctcacacc tattcctcct
      541 acaatcttcc cgtccacgac gatcggcagt ccgccctccc ggattacagc gttcgggaat
      601 gacaggatcc cgtactgccc cccgttgaga gcatcttcaa gagcttttgt cggccgctgg
      661 tataccaccg cggttcgacc cttcccgatc gcgagatcag cgcttgcaag cagcgtcccg
      721 tcggtccgct cgaagtagat cagatggcct ccgtcgtcga cgatcgcgat gtccatgttc
      781 cacttgttct tccgtgcttc cgctgccgca ccggcggcaa ccttcttggc atcctctaac
      841 gacagagcct ttttcgtcag gagttcaccg gaggctgtgc ctgcgatgag tattgtcgac
      901 aggatcaata caccaattcc tcccaacttt ctgagtgtcg cgatcatatg catccctcct
      961 ttagtcgtta ccgtaatcct tcatccagga ttcctcggag accatcaccg gtatcgcgac
     1021 gatttctacc ggaccgcttg tctgccaatc ggtgcctgcc cagttcccct cggggacaaa
     1081 ttcctgggtt gaagttagca ggacttgatc gctctgcggg gaatccccga ggatatccgc
     1141 catcgcccct cccgccagcg ctccgacaaa cgcaccaact gccaacatca aaatggtttt
     1201 tcgacccacg gcctgaccct cctttaatat caatacgtta tcgatgttca tacagctgtt
     1261 ctcattcaat ttttacaagt tcgtcgttat aaaagtgata atgaattttt gtgttacagc
     1321 caggccttga aagtgcaacg tggtactcag ttacatcacc cttttttgat tccaattcta
     1381 accacccgca acccgcctga gcaacctgcc catacaattc attaaaagcc atccccatac
     1441 gagggtgtat tggtacgagt tctgcctgcc gagtggcaac ttccttgtta aattggttcg
     1501 tgagattggc aacttggtcc tgcaacttcg ccacctgtgc ggctaacgac gcgacctgct
     1561 tttccaacga cgcgacctgc tcgggtaagg tacccccggg ttgtgcggac caacctatta
     1621 ccggaaccag aaagatgaaa ccgaccacca gggcgacaaa tgtacgtttc atttcgtcct
     1681 ccattcgtca aggggctgcg atttggggtt gcctcatgcc gtttggccct cgcctcccaa
     1741 attgccgccg cagtcggacg gtgggttacc ggttgtcgcg tttccttcag ggtgatttca
     1801 cggggtgcgg aaacggggtg ggcctcagtg gtaatgatta ttacatggag cgagtgacag
     1861 ataagataca gattgaaaag gaaaaataaa acacccacca ggcctcaaaa acccggtggg
     1921 tgtactctcc tcctcgaatc ggtctgagaa acataaacac aactcggacc gacatgaagg
     1981 gagcaggtca acaagacgaa acttcgatca acgacgctca cctccgccgc cacgcgttcc
     2041 tcccccgcca cgcgcttctc cgctgccacg cgctcctccg ccaacgatgc tggcacttcc
     2101 gcgtgacgaa aagctcctgg tctgtccgcc ttgtccgtag ccgctaaagg cacccgagcg
     2161 gacgctgctc tgacgccggg gttcggcata tcctcgagcc gcctgcctgt taccgttgaa
     2221 agacctggcc tcagttccgg gggtataggc acccgaacgg acgctgctct gaccgcgggg
     2281 ttcggcatat cctcgagccg tctgcctgtt accgttgaaa gacctggccg cagctccggg
     2341 ggtataggca cctgagcgga cgctgctctg accgcggggt tcggcatatc ctcgagcggc
     2401 ctgcctgttt ccgttgaaag ttcttgacgc agtgccgtaa cggttgtaaa ccccgccgcg
     2461 ccctcctcca cctcggtaaa aggtattccg gttataaaac gttctgctcc gggaatagta
     2521 cctgtcatgg tcatagattg cgtatcggtt atgccagtcg catccccaat gatcccaacc
     2581 ccagccaaaa ccgccgaacc aaccgattcc gaaaccgatt ccaaacgaaa gatagggacc
     2641 accaaaccaa attccaggat acggatacca ccccggccac gctacgattg gatacccgta
     2701 aatcaaccac ggatcatacg ctgggacgta aaccacatcg gggctcgcgg gctcaatgat
     2761 gatcgtcgag ccttgcgtgg ttacggtctg ctgcggggta gtcgtaagat tgccggattc
     2821 ttgcgcccgc tgacgcatca cctgcaccgc atccatcaca tcctgttgct ggttgtagta
     2881 agcgtcgcca agagatgacg tccaggaaag attcttgtcc atatttccca ggacggatgg
     2941 aaatgcagca accgccttta cgctcgggtc ccagggctgc tcgtccaaac cctgacccaa
     3001 agcttctccc tccaaatcag gatgcgcctg cacccatctg tcggcctcga cgacttgttc
     3061 ggggaacgtg gacgcctcca ggatctgcgc caccagcgaa tccggataca gcgcaatcgg
     3121 cgcaaccaac cgttgcaact gctcaggaga ctgttgcgtg tatggcggcg cctgcccgtc
     3181 cggtgccggg ggttgcgcgt cttgggccga catcttctgc ggccaggtcg cgaacaacag
     3241 actccacgac aacacggaaa ccaatatctg cttgcccaat cggggccagg agaacccagg
     3301 gacacgcccg aaaatattca tgttctttat cctcccctgc agtaactact cttatgacta
     3361 aataggcgca ttattcttaa gataagttac agtagatgtt tcttgatgca atgaactgcc
     3421 tttgacctgc ccccctgatt tggatcaaca tttggatcaa ccgtgcgagg ggattttcac
     3481 ctattgacct cccctcaaat ttagacgaac tggatgcggg gatattcgac tcccggtcac
     3541 aaccttcggt ttggacccca tccgatttat tacgcgtcct atatcctagg ttaacgatgc
     3601 ttcacagggt ccgcttgaag gtcagaatcg cgatcgtcat caggacgatg gtgaagaggg
     3661 agagaaacag gatatctccg gagatgctcg taataccggc gttcttgaaa agaatcgatt
     3721 tcagtgcatg gacagcgtac acctcgggat tacactttgc gaaagccttc agccatcccg
     3781 ggaaactttc tatgggataa accgcgccgc tcgggaaaaa gagaatgaca ttgaggaacc
     3841 cgctgatgat tccgacaatt ctcggatgcc gggccctccc caaaatgacg aacatgaggc
     3901 tgagcagaca caaggtagta aggaccacga cagaaaagag ggggaaaaac tgatccaacc
     3961 ctatcgctaa aggtataccc gtcaacaaca tgctcgtcac cgtgatgatc gcggcgatgg
     4021 ccgtggtgat cgaaagaccg ctgatgacaa ggccgctaac gatatgtgtt ttggtaaggg
     4081 gggtgagaag atagctctcc tcgatgccga gaaaacggtc catcacgagg ttgaacaccc
     4141 cagatgtcaa ggtcccaaga aaaatcgcca tgatgatgac gcccggcacg agggactgat
     4201 aatagtcgac cttggggtaa agattgatat ccctaagcag cacttcaccc gtcttttccc
     4261 gtatcggcac gtactctgag cgtattgaaa acagtgcggc gcttaagaga ttccgtatcg
     4321 tttcggaaga gatgttatcc gggttatcta gaaagaggcc aacctcgggt ctggcctgca
     4381 gcgacatttt tttgctgaaa tcgacgggta tgatgaccgc agctttatat ataccctctt
     4441 ttacgccatt gacggcttcc ccctcgctta gcaggttcgt gatccggaat gtttttgggc
     4501 ccgcctggat ggcacggaga ttttccatca cccgctttgc atatggaccc gaatcctggt
     4561 tcaccacggc caccgggagg tctgtcagtt ttccctggaa ggaattcccg aggatcacga
     4621 gatagatgat cggcatcaag atgctcatgg cgatcacgac cggattgcgc ttgaatttga
     4681 tcaggtccct ttcgatgact gccaacagtc ttctcatctt ccgaaactct gcggtactcc
     4741 ggcgccgacc agaaagctga ccttcttcac ctcctcttcc cggatgcttt tgccagtatg
     4801 gtgtatgaag acatcctcca gggactgttc atggaccgag gcggacagga tagtgccgcc
     4861 cgcctgcctg acatcatcaa tcaggaccgg cagatttttc gaaccattat cgacatagac
     4921 ccgcagggaa ttctcttccg cgatcgcttt gtgcacgaat ggcagctccc cgatcgcgcg
     4981 gaccagttcg ggagaaaggt ccccgaccag gagcgacacg atgtcgtttc cggggatgtc
     5041 cgctttcagc gcagagggag ttcccatggc gatgatcttc cccgcatcga tgatcgccac
     5101 cctgccgcac aatgtttccg cctcctccat gtaatgagtc gtcaggaata tcgtcatgga
     5161 atccccctcg atcaacttct tgaggaaatc ccagacgacg cgtctcgatt ggggatcaag
     5221 gcctatggta ggctcgtcca aaaaaagcaa tgtcggcctg tgaacgagaa cccgggcgat
     5281 ctcgagtctt ctcctcatcc ctccggaata tgtcgcgaca aggtcgtttg ccctttgtgt
     5341 gagccccagc atctcgagaa gatagcgaat cctctctttc cgttctcgtc cgcggatgcc
     5401 gtagaatcgg ccatagagat ccatattctc gtggcctgta agatccaggt cgcttgtcat
     5461 cgcctggggg accacaccga tgcgctttct cacctcggcg ggttcttccg agacgtcaca
     5521 gccggaaacg aacgccttgc cgttcgttgg cctgagcagc gtggtgagca tccggataag
     5581 agtcgttttc cctgccccgt tgggaccgag aaaaccgaag aattcgccct cgtccacctc
     5641 gaaactcacg tggtcgacag caacgatgga accgtattcc ttcgttatgt ccgatacaac
     5701 gattgccttt cccttttgca tcgccagatg ctccggccgt ctcaggtttt cccgggtatc
     5761 tcgacatcca ccgtcatccc gggtttcagg atccccgtgg ggtcatcaac cctcaccttt
     5821 acccggaatg ttttgatgtc ttccctgccc cggaccacgt ccctctgggt cgcgaactcg
     5881 ccgcttcgtc ctatctccga tatcctccct tcaaatgttt tcacgggtac cccttccacc
     5941 cttatgaacg ccttgccgtt caagaccacc ctgccgattt tcgtttcatc gatgtcgacc
     6001 cttgcataaa gactgttcag gtcgacgatc gtaagaacgg ccatgcccgg gctgaccatc
     6061 tcgcccgtct cgatcgcctt gaatatgacc gtgccggtta tcggcgtaac gatggacgta
     6121 tcatcgagtt tcgatcggaa aaatgcgcga tttgcttcgg cctccttcag catggccttg
     6181 gacgagttcc attggctgat cgcagcattc aattgactgg ccgcgacatc cttcctggag
     6241 cgagcagcat tcagcttctc tttcgacgaa tcgtatgccg ccacgttgac tgcatattcc
     6301 gtgaccatct ggtcgcggga ttccttggcg atcagattct ttttgtagag atcatctcta
     6361 cgatcggctt gtcttctcga ctcttccatc tgggcaccgg ccttctccag atcggccgcg
     6421 gcactcttca catccgcttc ggcagcgagt atgttcgccc tgccgttctc tatcgaagcc
     6481 tcggcaacct tgacgccggc tttcgcccgc tctactccgg ccatcgcctg ttccacggat
     6541 gcttttatat cgtcgctttc gagggtgatc acaacctgtc cggcttgtac cttgtcgccc
     6601 tcattgcaag atatccgtga tattttgccg gcgactttgg aagagagatt cacttcgact
     6661 ccgtcgatga tcccggttac atggaaaacg gggggttttc tgttccgtaa tagaagggaa
     6721 acacaaatag ccaagaggac cacaacaaag acgacgacca gggcgataac ccgcttcgtc
     6781 cactttttca tcgattttcc atgatccgcc agacttcttc tctcccagga tccactcaga
     6841 cccctgaacg gacgagcaga accgggactg cgctattgcg aaccacggac tccgcgtcac
     6901 tcccgagcgc cagatggctg aggccacgac gtccgtgggt tcccattacg atcaggtcgg
     6961 caggccacgt tttcgcctca tcgaggatca gaaaggatac ccggcgttca gcactctccc
     7021 gcatgacgga ttccacctgc actccgctct ttcgtgcctg ctcggcggct tcatccagaa
     7081 tctccttccc attatcacgt aatttatcta ttaaatcagg caaatacatg aatcccatat
     7141 aattggcggt aaggtaatgt tcatcgacca caaagagaca ccgcaatctt gcaccctgct
     7201 cctttgcgag tcggcatgct tctgtgaacc cacgcttcga agtttcgcta ccgtcgatgg
     7261 gtacaagaat tttcccatac ataatgaatc ctccgggctg tcgcgcaagc gcgtagaaaa
     7321 ttcggatgat ccgatcatta acaataatta tattccgatg aggaaagtac ggggaataat
     7381 gctggaactg cagatggtct gtttctgggg aacaggtcag gtcaaacgaa catctgcgat
     7441 tgcgataatt tcaatatttc aaaaatcttc tccgcctctt tggcggtaat gcgtttctgc
     7501 agccgctcca ggtagagtgg aaaatgccac cggctgatga tttccactcc cctcacctgt
     7561 tttcgtaccc ggacatccgc attggcaaac aggagcactg gttgtggttt aggtgctgat
     7621 atcccctggc tagtgagaag atctcttagg gataatgcct ccctccatcc ctgggtgata
     7681 aagtccttct cgaagggttt cccatcgcgc ttcaatatct ccccttcaca gctgacaact
     7741 cccctgtggc ttttcgtctc gatggtgaag atccctttcg tggagatgac gatatggtcg
     7801 atgttccctc ttcgcaatac gaagtcattc acgacgaaat atcctgccgg gagttcgccc
     7861 agtaagttgc caacatcttc ttcggcggcc gcgccgcggc gcgcatcaag ggctctcttg
     7921 gagtacacct ccgccttgtt gcccatcgct tttacccaat agatgaaacc acacgcaacg
     7981 acgagtacca ctgggagacc aaaggttgcg accgtcttgc tgttcttgct gtaaaggtag
     8041 atgcccgcga acatgacggt cagtaagatg agcgcagcca gccaccgggt ggcttccatc
     8101 tttcggacgc ccactcccgc cttgttgtaa accgtcgctt tcgtcgaacc agatcccgcc
     8161 atcctcttcc ctttctccga aatctgaata ccccccaaac aggggggaaa caccggaggc
     8221 acattctgga cgcataaatc catgcggcca ggccttcttc tcctaatctc ttttcggata
     8281 aatcggttat gaagtttaga ggaatcgctt acttttatag tcgtccgcgt gttcatgtaa
     8341 aagcttccag gggtggttga tcgctggctt tgatcctgaa tcgctaagtg gggcagaaag
     8401 gcttgaccat ttcgtagaat gtgcacttgt ggcagtcgcc gatcttctcg gccacggggc
     8461 aataaacctt tgtctcggat aatgtgcctg ccacggacca acagattcgc ccgtaattcg
     8521 ggaaagccgg gcatctctcc gtggcattct cgcttttttc cacaccacac cgcatgattt
     8581 cccagcactg tgccttgaat atctcgtcca ccggaagata ggggaggaag aggctgaaca
     8641 tcgcgcctcc tcccggtgcc gtggacaccc ggatcgatcc ccggtgttcc tccatgatct
     8701 tcttgcagat ggagaggccc aggcccgtgc cttggcctat ctccttggtg gaatagaacg
     8761 gttcgaaaat cctttttaca agatcaggcg atatgcctgg tcccgtatca gatacatcta
     8821 tgaccacgta gtgggtcccg ttttcgcaag ccgtccttgt cgccagggtc aacgttccgc
     8881 catcggtcat cgcgtcgatc gcgttggtca gcagattgtc gagggcctgc cggatctggt
     8941 cttcgtcgac gatacagggt ggcaggcctg gtgccggccg aaccgcgaag aggagatgct
     9001 tctcccgaca gattccgtcg aacttcgctt cctcctgggc gagaagatgg ttcatgtccg
     9061 cggattggag atgatatttt gcttcccggg aaaaggtcag ggtgtcccga aggatccgtt
     9121 cgagccgagc cacttccttg acgacaatct ttgcgtattc tttttccgcg gtattctccg
     9181 gaaggcgctt ctcaaggcgc tttgcgaacc cgccgatagc ggtcagggga ttgcggatct
     9241 catgagccac gtcagcggtg agccttccga gtgccgataa cttctccgct tccacaagct
     9301 tcccttgaag gtgcctcaag gtttcaagtg actcggccag attccggttg agcgattcga
     9361 tcttctcctt gtccctgcga aggatcgcag agagcaaacc gatcgggagt gcgatgagaa
     9421 aaaggaagga aatgcgaagc agaaagtcga tcggttcgat cgcagaaatg cgaaggccgg
     9481 cgaagaggta gatcacagtg cacatcgcgg ccacgacaag gccgctgcga tagccgtaat
     9541 aaaaggcatg gagggcagtg agaagataaa agcctatgaa gaagctgctg gcaaaccctc
     9601 cggagtttac gatcaggagg tagacaaatg ccaggtcgaa gaggaacgag agccgatata
     9661 tctccctctt cctgtcgaag cggaacaaga ggagcgtgta gatcagcag
//
LOCUS       DASSLD010000623         5807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_338338,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000623 DASSLD010000000
VERSION     DASSLD010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338338"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VFK23_11260"
     CDS             complement(<1..997)
                     /locus_tag="VFK23_11260"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET7319705.1"
                     /translation="MAFTGELEHLPIVDIIQLVHSTRKSGIFSVKGDKGGESKIVFSN
                     GSIVGANHINDTVRIGTVLVKTGAITIDDLKQAMGEQKTGKNRLPLLVTLIQMGKLKR
                     EDAVRGLKKLVELTIIELMGWTKGTFTFDTDATVVSSEEQDLGVDAQMVLMDALRILD
                     ERERDRAAGKEVPSFVELYADVLPEERAGETEREPPTITADDLGLADLDRLEKKIPGP
                     VSGMETFDPAAIQRQKIRELLPGFSFEEQEEFATFLRKAAGRKAAPDAAKQASRAVVL
                     FSGDTLISHSVISLCNDGGIPVFATDDEKELDRMVSQCHLSGRMAVVVFDTPAGPE"
     gene            complement(1236..1994)
                     /locus_tag="VFK23_11265"
     CDS             complement(1236..1994)
                     /locus_tag="VFK23_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HET7319706.1"
                     /translation="MKHIRAIIADDEEALRQHLKKKLAALWPELDLAGEAGDGEAALR
                     LIHDEAPDIAFLDISMPGLTGLEVAKRAAGRCLFVFVTAYDKYAIDAFESEAVDYLLK
                     PISEDRLEKTVKRLRERFAAAPSLPDISAALEKVSRSLEKSSSHLQWIKAQQRDGIKL
                     IPVADVYYLKSTDKYTSVRTRDGEYLIRKTIRELEEELDPELFWRVHRAAVVNVKSIH
                     AVNRSVAGTYTIRFRDIPDRLTVSRAYAHLFKQM"
     gene            complement(2040..3086)
                     /locus_tag="VFK23_11270"
     CDS             complement(2040..3086)
                     /locus_tag="VFK23_11270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET7319707.1"
                     /translation="MADDFIRIDPSRLTPRGILLSLAVNAVVNTVIAVILTAIGFGRG
                     FAVNLIFSQCIGNSIYLANLAAIPVYRRAKSLPAQIAIIVLSIISGAVIGVVIGAAAN
                     GMGPGVFLREQSTLFGQVVLLALLFGFVVSYVYISASVISGDKVRRIETEKNAVEAEL
                     RLYQSQMEPHFLFNTLSNVLGLIDSDRDKARRMLESFTSFLRFSIATARERTVSLDQE
                     MDIVRKYLDVFSVRMGERLRFDTSVPADLKSIRIPPLTIQPLVENAVKHGLEPKVEGG
                     AISIKAERDGGKVRITVADTGIGVSEKNAGSGIGLENIRKRLNLRYAERGRLLFEENE
                     PSGVKVTIEIPYER"
     gene            complement(3267..4622)
                     /locus_tag="VFK23_11275"
     CDS             complement(3267..4622)
                     /locus_tag="VFK23_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319708.1"
                     /translation="MEQTKGFFWEQHAGAERACLDILADCRRNNLTLARFEQQLIRKT
                     SSRLLDWVDHLLIRDSDHTRDKLAGLGFIKQPDIRHEALFHPGALLPRIVLSGNSGPD
                     SGVALRVESIADFFQSNGFLADIEGSPFSPYRRALVGKENQVAFWAVERRGSSGYEPV
                     DLQDAYLRSYLDGLEAWQNRSRGGEDEDRLFLEIFRIADRLIEQLGADLASHVVCRAE
                     RTYWLSRNYAGRAQQGRHETLGLGWGNHDHHTFRSSRRNFSRLVYLFSRLGFLNRERF
                     YAGAEAGWGAQVMENPAAGLTLFLDVDLSPEEVETDFSGTELKEREQLGTVGLWCALH
                     GDSILHAGMHHLAARFEFESLIADIAKLNVPYMAPFSDFPYLKQAFSVAEKWAVDPGR
                     VEKLVHEKTITAEQGEQFLSQGAVGSHLENIERREGYKGFNRKNVSAIIRETDPRRHL
                     A"
     gene            complement(4788..>5807)
                     /locus_tag="VFK23_11280"
     CDS             complement(4788..>5807)
                     /locus_tag="VFK23_11280"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7319709.1"
                     /translation="IPERFREKLTDLERRIKADDEARAVLESRMNMKTASGYNLFAFL
                     TDLSPGRRIAKLLAGSVGTLGLITRAVFQAEVHEREAAAVLLYFDGLAEAGRAVSSLR
                     ELDVAAIELISRETIRIMRDRTSLPGELAPDAHLLFVELAGPEVQKKVKSVINLFHHD
                     GYRMSAAPAVVMSQDGIEGLWKLRKQILWLIEHPQPGLRALAVINDVGVPVDRLAEFI
                     YDVQKVFARHGTLTLVYGHAGNGNLHLRPLFDLSLPDIRGRVRQLADDIYDVVFRHGG
                     TVTAEHGMGRLRAPYLKREWGEALYNYMREVKRIFDPEEILNPGVMFSDAPITDHMRE
                     DLLEP"
BASE COUNT         1180 a         1707 c         1675 g         1245 t
ORIGIN      
        1 cctccgggcc cgcgggggtg tcgaagacga cgacggccat tcgcccggag aggtggcact
       61 gcgataccat ccggtcgagc tctttttcat cgtcagtcgc aaagacgggg atcccgccgt
      121 cgttacagag ggatatcacg gaatgactga tcagcgtatc gccgctgaac aggacgaccg
      181 cccggcttgc ctgcttcgcc gcgtcgggcg cggccttgcg tcccgcggct ttcctcagaa
      241 aagtagcaaa ttcctcctgc tcctcgaaag aaaaacccgg aagcaattcc cttattttct
      301 ggcgctggat tgcggccgga tcaaaggttt ccattccgga aacgggcccg ggtattttct
      361 tctcaagccg gtcgaggtct gcaagtccga gatcatccgc ggtaatggtt ggaggctccc
      421 tttctgtttc accggctctt tcctcgggta gaacatcggc atagagctcc acaaaggaag
      481 gaacttcttt tccagccgca cggtcacgtt cccgctcatc caggatgcgc agcgcatcca
      541 tcaaaaccat ctgggcatcg actcccagat cctgctcttc cgaggaaacg acggtcgcat
      601 ccgtgtcgaa cgtaaacgtg cccttcgtcc agcccatcag ctcgattatc gtcagctcga
      661 ccagcttttt cagtcctctc acggcgtctt cccgcttcag cttgcccatc tgtatcaggg
      721 taacgagcag cggcaggcgg tttttacccg tcttctgctc gcccatggcc tgcttgagat
      781 cgtcaatcgt tatggcgccc gtctttacca ggacggtccc gatgcggacg gtgtcgttga
      841 tatgatttgc accgacgatt gacccgttgc tgaagacaat cttactctcc ccccccttgt
      901 cccccttaac ggaaaaaatg cccgacttcc gcgtcgagtg gaccagttgg ataatatcta
      961 cgataggcag atgttctaac tctccggtga atgccatgtt ccctcgtata aatccctgga
     1021 aagtatcccc tcaggggggg tgaaatatga gccgggaaag cattggtttc ccgagcagga
     1081 taaaatcggc aatcgtacgg ccgcggagac ttcctgcgcg tggaacggga ttatccaaat
     1141 atgcagtcgg cttgaattat ataatgatag ggaataagaa tcaagtacgc tttcacaact
     1201 tcgcgaggtg ccgcaaacgt gagttcccgc tctgcttaca tctgcttgaa caggtgcgcg
     1261 taagcccggc tgacggtcag gcggtcgggg atgtcccgga accggatggt gtaggtcccg
     1321 gctacggagc ggtttacggc atggatggac tttacgttca cgacagcggc ccggtgcacg
     1381 cgccagaaca gctccgggtc gagctcttcc tccagttccc tgatcgtctt ccggatcaga
     1441 tactctccgt ccctggtacg gacggatgtg tacttgtccg tggacttgag atagtagaca
     1501 tcggccacgg ggatgagctt gatgccgtcc ctctgctggg ccttgatcca ctgcaaatga
     1561 gaggatgatt tctcgaggga acgcgagacc ttttcgaggg ctgcggaaat atccggaagc
     1621 gacggggcgg cggcaaagcg ttccctgaga cgttttacgg ttttctccag acggtcttcg
     1681 gaaatgggct tgaggagata gtccacggcc tcgctttcga acgcatcgat cgcgtatttg
     1741 tcgtaagccg tgacgaagac gaaaagacac ctgccggccg ccctctttgc gacctcgaga
     1801 ccggtcagac ccggcatgct gatgtccaga aaagcgatgt ccggagcttc gtcgtgaatg
     1861 agcctgagcg cggcctctcc gtccccggcc tctccggcga gatcgagctc cggccagaga
     1921 gcggcgagct tctttttgag atgctgcctg agcgcctctt cgtcgtcagc gatgattgcc
     1981 cggatgtgtt tcatgatagg ttccaaatcg tcacagagcg acagggttca tgggggaaat
     2041 catctctcat agggaatctc gatcgtcacc ttcacgcctg aaggttcatt ctcctcgaac
     2101 agcagcctcc cccgctccgc gtaccgcaga ttgagacgct tcctgatgtt ttcaagaccg
     2161 atgccgctcc ctgcgttttt ttcgctcact ccgatcccgg tatcggccac cgtgatcctc
     2221 accttgccgc cgtcgcgctc tgctttaatg gatatggccc cgccctcgac cttcggttca
     2281 agaccgtgct tcaccgcatt ctcgacgagc ggctggatgg taagcggcgg aatgcgaatc
     2341 gacttcagat cagccgggac cgaagtatcg aatcggagcc gctcgcccat gcgcacggaa
     2401 aatacgtcga ggtatttgcg gacgatatcc atttcctgat cgagggaaac cgttcgttcc
     2461 cgtgccgttg caatcgaaaa gcggagaaag gacgtaaacg attcgagcat gcgccgggcc
     2521 ttgtcccggt ccgaatcgat caggccgagg acgttcgaga gggtgttgaa caggaaatgc
     2581 ggctccatct gcgactgata gagtctcagc tccgcctcca cggcgttctt ttcggtctcg
     2641 atccttcgga ccttatcccc ggagatgacg gaggcggaga tgtagacata gctcacgaca
     2701 aacccgaaca ggagggccag gaggaccacc tggccgaaga gggtcgattg ctcgcgcagg
     2761 aaaacgccgg ggcccatgcc gttggcggcg gctccgatta cgacgccgat tacggcaccg
     2821 gaaatgatcg acagcacgat gatcgcaatc tgtgcaggaa gggactttgc ccggcggtag
     2881 acggggatgg ccgcgagatt tgcgagatag atggaattgc cgatgcactg ggaaaaaatg
     2941 agattgacgg cgaagcccct tccgaaaccg attgccgtga gaatgactgc aatgaccgta
     3001 ttgacaaccg cgttcacggc cagggagagg agaattcccc ggggtgtcag acgggagggg
     3061 tctatgcgga taaaatcgtc ggccatattg ttatccgtca gctactgtat cacggttgca
     3121 tcgtcattcg agtgtaatgc gtccaacaca tcttagattt gcattatcag cgtatgtcat
     3181 tcccgcaaaa gcgggaatcc atctgattaa attggattcc gggtcaagcc cggaatgaca
     3241 acaaaaggaa aaggatttat gccgcgttac gccaggtgtc ttctcgggtc ggtctcgcgg
     3301 ataatggcgc tcacattctt tctgttgaaa cccttgtacc cttccctgcg ctcgatgttt
     3361 tccaggtggc tgcccaccgc gccctgggaa agaaactgct cgccctgctc agcggtgatg
     3421 gtcttctcgt gcaccagctt ctccacccgc cctggatcga cggcccattt ctcggcaacg
     3481 ctgaaggcct gcttgagata ggggaaatcg ctgaaggggg ccatgtacgg gacgtttaac
     3541 ttggcgatgt cggcgatgag gctttcgaat tcgaagcggg ctgcgagatg gtgcatgccg
     3601 gcatggagga tggaatcgcc gtgcagtgcg caccagagcc ccacggtgcc gagctgttct
     3661 ctctctttga gctccgtccc ggaaaagtcc gtttccactt cctccggaga gaggtccaca
     3721 tcgagaaaga gggtgaggcc cgctgccggg ttttccatga cctgggctcc ccagcccgcc
     3781 tccgcccccg cgtaaaagcg ctcgcggttc aaaaaaccca accgcgaaaa gaggtacacg
     3841 agtcttgaga aattccgccg ggaactgcgg aaggtatggt ggtcgtgatt tccccatccc
     3901 agaccgaggg tttcgtggcg gccttgctgc gccctgccgg catagttgcg ggacagccag
     3961 taggtgcgct cggcccggca gacgacgtgg gaagcgaggt ccgcgcccaa ttgctcaatg
     4021 agacggtccg caatccggaa gatttccaga aagagccggt cctcgtcttc acctccccgc
     4081 gatcggttct gccaggcctc cagcccgtcc agataggaac gcagataggc gtcctgcaga
     4141 tcgaccggct cgtaaccgct gctccccctc ctttcgacgg cccaaaaggc gacctgattt
     4201 tccttaccca caagggcgcg gcggtagggg ctgaaggggc tgccctcgat gtcggcgagg
     4261 aaaccattcg attgaaaaaa gtcggcgatg ctctccaccc gcaaggccac gcccgagtcg
     4321 gggccggaat ttcctgacag aacgattctc ggcaggagag cacccggatg gaacagggct
     4381 tcatgcctga tatccggttg tttgatgaat ccgagaccgg cgagtttgtc tctcgtatgg
     4441 tccgagtcgc gaatgagcaa gtggtccacc cagtcgagaa ggcgggaact ggttttccgg
     4501 atgagctgct gttcgaagcg ggcaagggtg aggttatttc gtctacagtc cgcgagaatg
     4561 tcgaggcagg ccctttccgc ccccgcgtgc tgttcccaga agaaaccttt cgtctgttcc
     4621 ataatcgacc tcacaagtta aagacgttgt aaccacgggc gactgcggcg cacacggaaa
     4681 aaccgggttc aatgttcccg atttactaat taagaccgtg tttattcgtg tgttcgcgca
     4741 gcgaacccgt ggttcccggt tgatctccgt ggttcaatcc cttcgtctca cggttccagc
     4801 agatcctccc tcatatggtc ggtgatcgga gcatcgctga acatgactcc ggggttgagg
     4861 atctcctccg gatcgaagat cctcttgacc tcccgcatgt aattgtacag cgcttcgccc
     4921 cattcccgct tgaggtaggg cgcccgcaac cttcccatgc cgtgctccgc ggtcaccgtg
     4981 ccgccgtgac ggaatacgac gtcgtagatg tcgtccgcga gctgacggac gcggccccgg
     5041 atgtccggca gcgacaggtc gaagagcggg cgcagatgga ggttgccgtt tcccgcgtga
     5101 ccgtagacga gggtgagggt cccgtgcctg gcaaagacct tctggacgtc gtaaatgaat
     5161 tccgccaggc gatcgacggg gaccccgacg tcattgatca cggcaagggc acggagaccg
     5221 ggctgcggat gctcgatcag ccacaggatc tgttttcgca gcttccacag cccttcaatt
     5281 ccgtcctgcg acatgacaac ggccggcgca gcgctcatcc ggtagccgtc atgatgaaag
     5341 agattgatca cggattttac ttttttttgt acttccggtc cggccagctc cacgaaaagc
     5401 aggtgcgcgt ccggggccaa ctccccggga agagacgtgc ggtcccgcat gatgcggatc
     5461 gtctcgcggc ttatgagctc gatcgcggcc acgtccagct cgcgaagtga ggagaccgcc
     5521 ctgccggcct ccgccaggcc gtcaaagtac agcagcacgg ccgcagcttc ccgctcatgg
     5581 acctcggcct gaaaaacggc gcgggtgatg agaccgagcg ttcctacgct gcctgccagg
     5641 agcttcgcta tgcgccgccc cggagacaga tcggtcaaaa aagcaaaaag attatagccg
     5701 ctggccgtct tcatgttcat gcgggattcg agaaccgccc gcgcctcgtc atcggccttg
     5761 atccttcgct ccaggtccgt gagcttctcc ctgaaccgct ccgggat
//
LOCUS       DASSLD010000624         3945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1352251, whole genome shotgun sequence.
ACCESSION   DASSLD010000624 DASSLD010000000
VERSION     DASSLD010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352251"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFK23_11285"
     CDS             <1..645
                     /locus_tag="VFK23_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7319710.1"
                     /translation="VESILPENQVILTVGIGMVLANLATIIFTADYRSTPVDYATKAW
                     YLSDFWKKAPLELSLSFPWSVSFVIAVLITVGLWFFLTRTDTGKSIRATAQDKDAAIL
                     MGVNVGRVRVLTFGLGSSLVGAAGCLFIPIYYIYPDIGGQFTLIAFVITILGGLGSTV
                     GAIIGGLILGIFESLTATYVGMGWAPVGRFVIFLAALVFLPGGVASLLKTKRLK"
     gene            797..1735
                     /locus_tag="VFK23_11290"
     CDS             797..1735
                     /locus_tag="VFK23_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7319711.1"
                     /translation="MKKYLPFIIAGVLALVPLAGPSSYVMHILILVIMWSVIGMAWNL
                     LSGYCGQVSFGHAAFFGTGAYTAGMLYSKFGISGWWGLPVSIVVVSAFALVIGYICLR
                     LRGPFFALATLSMGVILRIVTENMVGFTGGDLGIMIRERTWVEKTWYYYIILLIAGAT
                     FIVVKKVIESKLGYYFVAIREDQDAAESLGIDTTHYKTVALVLSAVLTGFAGAFYTNY
                     MGYIDPKVVFALHDISIVTIMVVMVGGVATYWGPVVGAVIMVLLAEAIRSIPTLGTAH
                     QTFFGILLIVIIIFLPNGIVGDFPKLKRLVGAGRHP"
     gene            1732..2454
                     /locus_tag="VFK23_11295"
     CDS             1732..2454
                     /locus_tag="VFK23_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7319712.1"
                     /translation="MSFLEVRKVSMFFGGLAAIKDVSFGVARGEIVGLIGPNGAGKTT
                     IFNVVNGFYQPSRGDVLFKNERISGLKPHQICGRGMGRTFQVVRPLQRMSVMDNVIAS
                     AFLRSPDKKHAEETAREILHFTGLYDDRDVLSKGLPLGKRKRLEIARALATRPELLLL
                     DESCAGLNASELDESIAIIRKIKEQGITIMIIEHHMKVIMSISDRIVVLNYGEKIAEG
                     TPQEIQCNTQVIEAYLGEAQCA"
     gene            2447..3151
                     /locus_tag="VFK23_11300"
     CDS             2447..3151
                     /locus_tag="VFK23_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7319713.1"
                     /translation="MLKVRNIHVFYGDVQVIWDISFEVKQGEVVALIGANGAGKSTTL
                     KTISGLLRPNRGEVLFAGSPIHTVAPYRLVEMGLAHCPEARRLFVEMTVEENLDMGSL
                     RGEARKNRNKTKERVFELFPRLRERRRQPAGTLSGGEQQMVAIGRGLMALPKLLMFDE
                     PSLGLAPILVREIFNVIEAIRKEGTTVLIVEQNVKQTLEIADRAYVLETGKVVLEGTG
                     KDLLNDPHVKTAYLGL"
     gene            3263..3907
                     /locus_tag="VFK23_11305"
     CDS             3263..3907
                     /locus_tag="VFK23_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HET7319714.1"
                     /translation="MYVSDWMTKKVYTVNADDYLSDAVLLMRERGIKHVPVLKNGKLK
                     GIVSDRDIKEYSPSKATSLDIYELHYLLAKTRIREIMKTSVVTTSADTPVEEAAMVML
                     DGNIGCLPVLDGGGLIGIISDRDIYRALVDITGIRHGGNRICLVIEDRPGSIREVGDI
                     IRKNGYHLQSILTSYEGIKEGYREVVIRTAGSGNFKGLKDELESFYKNVQIRKG"
BASE COUNT          815 a         1134 c         1174 g          822 t
ORIGIN      
        1 gtcgaatcga tcctgcccga aaaccaggtg atcctgaccg tcggaatagg gatggtcctg
       61 gccaatcttg caacgatcat cttcacggcc gactatcgtt cgaccccggt cgattacgcc
      121 accaaggcct ggtacctctc ggatttctgg aagaaggcgc cgctcgagct ctctttgtcc
      181 tttccctggt ccgtgtcctt cgtcatcgcc gtgctcatca cggtcggcct gtggttcttt
      241 ctcacccgga cggacacggg caaatccatc cgggcaacgg cccaggacaa ggacgcagcg
      301 atcctcatgg gcgtgaacgt gggcagggtg cgcgtcctga ccttcggcct gggttcatcc
      361 ctcgtgggcg cggcgggttg cctcttcatc ccgatctact atatctatcc cgacatcggc
      421 ggacagttca cgctgatcgc cttcgtcatt actatattgg gggggttggg ttccacagtg
      481 ggggcgatca tcggcggtct gattctcggt attttcgagt ccctgaccgc tacctacgtt
      541 ggcatgggat gggcgccggt agggcgcttc gttatttttc tggcggcgct ggtattcctt
      601 cccggagggg tggcgtcact gctgaagaca aagaggctta aatgaagcag acactttgaa
      661 tggataaatt cttgttaatt tatttcgtca tcccctaatg aatttatcgg ggatccagtg
      721 cctctggtta aacctcattt cttaagaccc ctggattccc gctaagagca tgcgggaatg
      781 acgttgaatg cgtggtatga aaaaatatct tcctttcata atcgccggcg tcctcgccct
      841 ggttcctctt gccggtccca gctcctacgt gatgcacatt ctcatcctgg tcatcatgtg
      901 gtccgtgatc ggcatggcct ggaacctcct ctctggctac tgcggacagg tgtctttcgg
      961 acatgccgca ttcttcggca ccggcgccta taccgcgggg atgctctaca gcaagttcgg
     1021 tatctcgggg tggtgggggc tgccggtcag catcgtggtc gtatcagcct tcgcgctggt
     1081 catcgggtat atctgcctgc ggctccgggg ccctttcttc gcgctggcca ccctgtcgat
     1141 gggcgtcatc ctccgtatcg tcacggagaa catggtgggt ttcaccggag gggacctggg
     1201 catcatgatc cgcgagcgga cctgggttga gaagacctgg tactactaca tcatcctcct
     1261 tattgccggg gcaaccttta tcgtagtgaa aaaagtcatt gagtcgaaac tcggttacta
     1321 cttcgtcgcc attcgcgagg atcaggacgc cgcagagtcg ctcggcatcg acacgacgca
     1381 ctacaagacc gtggcgctcg tactctccgc cgtgctcact ggcttcgccg gagccttcta
     1441 cacgaactac atggggtata tcgaccccaa ggtcgtcttc gcactgcacg acatttcgat
     1501 cgtcacgatc atggtcgtga tggtgggcgg cgttgccacg tactggggtc cggtcgtggg
     1561 ggcggtcatc atggtgcttc tggccgaggc gatccggtcg atcccgacgc tgggcacggc
     1621 ccaccagacg ttcttcggca tcctgctcat cgtcatcatc atctttctgc ccaacggcat
     1681 cgtcggcgat tttccgaagc tgaagaggct ggtcggagcg gggaggcatc cgtgagtttt
     1741 cttgaagtgc gcaaggtgtc catgttcttc ggcggcctgg ccgccatcaa ggacgtctcc
     1801 ttcggcgtgg cccgggggga gatcgtgggg ctcatcgggc cgaacggcgc gggcaagacc
     1861 acgatcttca acgtcgtgaa cggcttctac cagccgtccc gcggggacgt ccttttcaag
     1921 aacgaaagga tttccggcct gaaaccgcac cagatctgcg gccggggcat ggggaggacg
     1981 ttccaggtcg tcaggccgct ccagcgcatg agcgtgatgg acaacgtcat cgcctcggca
     2041 tttctccggt ctcctgacaa gaaacacgcg gaagagacag cgcgggaaat cctccatttc
     2101 accggcctgt atgacgatcg cgatgtcctc tcgaagggcc tgccgctcgg aaaaagaaag
     2161 cgcctcgaga ttgcgcgtgc gctcgccacg cggccagagc tcttgctcct ggatgaatcc
     2221 tgcgcggggc tgaacgcgtc ggagctcgac gagtccatcg cgatcatcag gaagatcaaa
     2281 gagcagggaa tcaccatcat gatcatcgaa caccacatga aagtgatcat gtccatttcg
     2341 gaccggatcg tcgtcttgaa ctacggcgaa aaaatcgccg aaggcacgcc gcaggagatc
     2401 cagtgcaata cccaggtcat cgaggcctat ctcggcgagg cgcagtgtgc ttaaggtcag
     2461 gaacatacac gtattctacg gcgacgtcca ggtcatctgg gacatctcct tcgaggtgaa
     2521 gcagggagag gtcgttgccc tgatcggcgc gaacggtgcg gggaaatcga cgaccctgaa
     2581 gaccatatcg gggctcctgc ggccgaacag gggcgaagtg ctcttcgcgg gaagtcccat
     2641 ccatacggtc gcgccctacc ggctcgtcga aatgggcctc gcccactgcc ccgaggcgcg
     2701 caggctgttc gtggaaatga ctgttgaaga gaacctcgac atggggtcgc tcaggggcga
     2761 ggcgcggaaa aaccggaaca agaccaagga aagggtcttc gagctcttcc cccgcctcag
     2821 ggaaagacga aggcagcccg cagggacgct ctcgggaggc gagcagcaga tggtcgccat
     2881 cggcaggggg ctcatggcgc tgccgaagct cctgatgttc gatgagccct ccctgggtct
     2941 cgcgcccatc ctggtgcggg agattttcaa tgtcatcgag gcaatccgga aggaaggaac
     3001 gacggtcctc atcgtggaac agaacgtgaa gcagaccctc gagatcgccg accgggcgta
     3061 cgtgctggaa accggcaaag tggtccttga gggaacgggc aaagacctcc tgaacgaccc
     3121 gcatgtcaag acggcgtatt tgggacttta atgcaaatcg atggtaggag ggtaagaagg
     3181 tgagcgggtg ggaaaatgtc ctacatcaca ctatcctacc ttccaaacct cttatttccc
     3241 cgatttaaac cggaggtggc caatgtacgt ttctgactgg atgacgaaga aggtctacac
     3301 cgtcaacgcc gacgactatc tctccgatgc cgtcttgctg atgcgggaac ggggcatcaa
     3361 gcacgttccg gtcctcaaga acgggaagct caagggcatc gtctcggacc gcgacatcaa
     3421 ggagtacagc ccctcgaagg ccacgtccct cgatatctat gagctccact acctcctggc
     3481 caagacgagg atcagggaaa tcatgaaaac gagcgtcgtc accacgtcgg ccgatacacc
     3541 ggtggaggaa gcggcgatgg tcatgctgga cgggaacatc ggctgcctgc ccgtgctgga
     3601 cggcggcgga ctcatcggca tcatctctga ccgcgacatc taccgcgcac tcgtggacat
     3661 caccggcatc cgtcacggcg gcaaccgcat ctgcctcgtc atcgaagacc ggcccggctc
     3721 tatccgggaa gttggcgaca tcatcaggaa gaacggctat catctgcaga gcatcctcac
     3781 ctcctatgaa gggatcaagg aaggatatcg cgaagtggtg attcgtacgg cgggatcggg
     3841 aaacttcaaa ggattgaagg atgagctgga gtccttctat aagaacgtac agatccggaa
     3901 ggggtagccg gaaggaacag cgaagagaat cacccaacga gcttc
//
LOCUS       DASSLD010000625         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1343293, whole genome shotgun sequence.
ACCESSION   DASSLD010000625 DASSLD010000000
VERSION     DASSLD010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343293"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="VFK23_11310"
     CDS             complement(<1..1453)
                     /locus_tag="VFK23_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(848..850),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7319715.1"
                     /translation="MGVSRREFMKISGSTVLLGSLGVKLDPAADAKEQLRIKGATETK
                     TICPYCSVGCGIIVHVKDGKVVNTEGDTDHPINEGTLCSKGASLYQIINNPTRLTKPK
                     YRAAGATEFKEVEWDWALDEIAKRVKDTRDRTFKLTSKSKVKEKQPDGTEKDVEKEFT
                     VNRTDGIAHVGSAALDNEECYMLQKLVRSWGLVYIEHQARIUHSATVAALGESFGRGA
                     MTNHWIDIRNADVILVMGSNPASNHPISMKWVMKAKEGGAKLIVVDPRFTQTAAKADV
                     YAPMRSGTDIPFLGGMIKYILDNNLIFRDYVVNYTNASFLINPDFKGPGELNGLFSGY
                     DDKTRKYDKKTWSFQMDENGVPKKDPTLRDPHCVYQLLKKQYSRYTLDKVSSITGTPK
                     EKLEEVYKLYASTGKPNRVGTELYAMGWTQHTIGTQNIRAMCIIQLLLGNMGMAGGGI
                     NALRGESNVQGGTDYGILFHILPGYLPVPSASVT"
     gene            2052..2453
                     /locus_tag="VFK23_11315"
     CDS             2052..2453
                     /locus_tag="VFK23_11315"
                     /inference="COORDINATES: protein motif:HMM:NF016833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7319716.1"
                     /translation="MRYRYIALKYAGRTRPEASFQSIWDAFGTWPLHSTIVWHPATDI
                     YETLDKIIVVVELAGVSEDDVTVTLFSDLLVVEGSRKQPALEDMNACHQLGIKYGDFR
                     SEIALHAPIDHDTVKAEYKHGLLRITLDKVD"
     gene            2589..>3554
                     /locus_tag="VFK23_11320"
     CDS             2589..>3554
                     /locus_tag="VFK23_11320"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET7319717.1"
                     /translation="MKIHDILAKFIKKEGRSKSMETSQENSLEKTPSVPAELPLLPLK
                     DTVVYPLTVYPLVIGKEKSIKLINDVTVGDKILGLTAQKKVDIEVAGLTDIYTTGTMA
                     RILQMIKVPDGTLRVLVQGIERITITEFTQTEPYIKASVKPLPDKSQPSVELEALMRG
                     AQELFQKLVTLTPNMPEELSSAAINIEDPRQLAYLIATNIRLDLPQREEILETDLVID
                     KLTKLMQYLNREVEVLELGRKIQGQARDQMQKAEREYLLRQQLSAIRKELGEETDEGS
                     EIKNLREKIEEAKLPPEAEKEAKRELGRMEKLSGSSPEYSVIRTYL"
BASE COUNT          852 a          922 c          957 g          823 t
ORIGIN      
        1 tggtgactga agccgacggc acaggcaggt agcccggcag gatatggaaa agaattccgt
       61 agtcggtccc accctgcacg ttcgattccc cgcgcagggc attgatgcct ccgccggcca
      121 tgcccatatt gcccaaaaga agctggatga tgcacatggc ccggatgttc tgtgtcccga
      181 tggtgtgctg agtccagccc attgcgtaga gctcggtccc gacgcggttc ggtttgcccg
      241 tggaagcgta gagcttgtaa acctcttcga gcttttcttt gggggtgccg gtaatgctgg
      301 aaaccttgtc gagggtgtag cgcgagtact gtttcttcag aagctggtac acacaatgcg
      361 ggtccctgag cgtggggtcc ttcttcggga cgccgttctc gtccatctgg aacgaccatg
      421 tcttcttgtc gtattttctt gtcttgtcat catagcccga gaaaagcccg tttaattccc
      481 ccggtccctt gaagtcgggg tttatgagga acgaggcgtt ggtgtagttg acgacataat
      541 ccctgaagat gaggttgttg tcgaggatgt acttgatcat cccgccgaga aaggggatat
      601 ccgtgcccga ccgcatagga gcgtagacgt ccgccttggc tgcggtctgc gtgaagcgag
      661 gatcaacgac gatgagcttg gcgcctccct cctttgcctt catgacccat ttcatggaaa
      721 tggggtggtt cgatgcggga ttgctcccca tgacgaggat tacatcggca ttgcgaatat
      781 cgatccagtg gtttgtcatt gcaccgcgtc cgaacgactc tcccagagcc gctacagtcg
      841 cggagtgtca aatgcgcgcc tggtgttcta tatacaccag gccccaggac ctcaccagtt
      901 tctgaagcat gtagcattct tcattgtcca aggccgcgct ccccacgtga gcgatgccgt
      961 cagtcctgtt cacggtaaat tccttctcaa cgtccttttc ggttccgtca ggctgttttt
     1021 ccttcacttt cgacttagag gtaagcttga aggtcctgtc acgggtgtcc ttcacacgct
     1081 tggcgatctc gtcgagcgcc cagtcccatt cgacttcctt gaactctgtt gcgccggcgg
     1141 cgcggtactt cggcttggtc agccgggtgg gattgttgat gatctggtac aacgacgcgc
     1201 cttttgaaca gagggtccct tcattgatcg gatggtccgt gtctccctca gtgttcacta
     1261 cctttccgtc cttgacatgg acgataatgc cacagcccac agagcagtag gggcatatcg
     1321 tcttcgtctc cgtagcgccc ttgatccgga gttgctcctt ggcgtctgcc gccggatcaa
     1381 gcttgacgcc gagagagccg agcaaaacgg tactgccgga gatcttcatg aactcgcgtc
     1441 ttgaaacccc catatcggtt cctcccttta cgttatttta ttttttcgtg atgcggcaat
     1501 cgtagaattt gaagtgagag acgggtcgcc acccgtatga ggaagaatcg gatcaactgg
     1561 aatcgaaatc aggcgccggg tagctcatta agttttagca aaatgtataa gaaaaattaa
     1621 ttaaattatt agtgacatct attgtatagc agaaaattgt caatttgtcg agggaagtgc
     1681 ataaaaagtt tgttcgaaag agttataaat ggttaaattg ttatttactt cggaaacctt
     1741 ctcatgagcg tcctgaaatg gcgttctgca atggcgatga caactcgtcc ctgcttctat
     1801 cttctcttcg gaaaaaaatg acctgtgccg aagggaggcg ggctatctag aggttttttt
     1861 aagaaaattt ttcataaaag gatttcgttg tctgcacgcg atagccgaaa cagcacggat
     1921 taacgagaaa gattgcagca aaaacaggca cgccggctta ttttttgatt ttctttcgcc
     1981 ataaaacaag ctataatcac atgatacaat agattaaaga cattatatga tcgtaagagg
     2041 aggcagggaa agtgcgctac cggtatattg cactcaaata tgcgggccga acgcgacccg
     2101 aggcatcatt ccagagcatc tgggacgcat tcgggacctg gccgctccac tctaccatcg
     2161 tctggcatcc tgccaccgat atttacgaga cgctggacaa gatcatcgtc gtcgtcgaac
     2221 tggccggagt ttccgaagac gatgtgacgg tgacgctttt cagcgatctt ctcgtggtcg
     2281 agggttcaag gaagcagccc gccctcgagg atatgaacgc ctgccatcag ttgggcatca
     2341 aatacggcga tttccggtcc gagatcgcgc ttcatgcccc catcgaccac gatacggtga
     2401 aggcggagta caaacacggt cttttaagga tcacactcga caaagtcgat tgaggcgctg
     2461 agggaaaaac ttttccgcaa ggcacgaagt cacacaagga aaagagaata aaaattcttt
     2521 gagtcttgcc gtctttgagg ctctcttctg tttcagactt gtccgggttg gggcgcaaag
     2581 gcaaatacgt gaaaatccac gacatcctgg cgaaattcat aaaaaaagaa ggcaggagca
     2641 aatccatgga gacatctcag gaaaacagct tagaaaagac gccttccgta cctgcggaac
     2701 tgccgctgct gccgctcaag gacacggtcg tctatccatt gaccgtctac cctctggtta
     2761 tcggcaagga aaaatcgatc aagctgatta acgacgtgac cgtaggcgac aagatcctcg
     2821 gtttgaccgc tcagaaaaag gtggacatcg aggtggcggg gcttacggac atctatacca
     2881 cggggacaat ggcgaggatc cttcagatga tcaaggtccc tgacggcacg ctccgggtcc
     2941 ttgtacaggg catcgaacgg atcaccataa cggaatttac ccagaccgag ccctacatca
     3001 aggcgagcgt caagccgctg ccggacaagt ctcagccgtc agtcgagctt gaggcgctca
     3061 tgcgcggtgc ccaggaactg ttccagaagc tggtgacgct tactcccaac atgcccgagg
     3121 agttatcctc ggcggcgatc aacatcgagg acccgcgcca gctcgcttac ctgattgcga
     3181 cgaacatacg gctggacctg ccgcagcggg aagagattct tgagacagac ctggtcatcg
     3241 acaaactgac gaaactgatg caatacctga accgcgaggt cgaggtactc gaactgggca
     3301 gaaaaatcca gggccaggcc cgcgaccaga tgcagaaggc cgagagagaa tacctgctca
     3361 gacagcagtt gtcggcgatc cgcaaggagc tgggcgagga gaccgacgaa ggctccgaga
     3421 tcaagaacct gcgggagaag atcgaagagg caaaacttcc tcccgaggct gagaaagagg
     3481 ccaagcgcga gctcggccgc atggaaaaac tctccggttc gtcgcctgaa tattccgtca
     3541 tccgcacgta cctc
//
LOCUS       DASSLD010000626         7416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_634093,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000626 DASSLD010000000
VERSION     DASSLD010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634093"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..680
                     /locus_tag="VFK23_11325"
     CDS             282..680
                     /locus_tag="VFK23_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7319718.1"
                     /translation="MNAGGDKGEKTILLVDDDKGNRTIFAEILTDMGYMVIDKPDVTS
                     ALEVVRNEMPIDLVITDYRLPDTDGFTLVDAVRKARPGVQIIMMTAYGNAENYIRSMS
                     LGVFEYLNKPVKKKEFERIVSFALSKSASA"
     gene            complement(702..1736)
                     /locus_tag="VFK23_11330"
     CDS             complement(702..1736)
                     /locus_tag="VFK23_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007546433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7319719.1"
                     /translation="MKALYFDEQKGYQGIKTGEFPVPEITESEVLVRVKAFSLNHLDV
                     WVMGGDYPVKIPLPHVFASDAAGVVEKTGAGVHHVKQGDEVILFPGLSCGHCERCLSG
                     RDNECAGFSIIGVAAQGVSAEYVKAPGDNIFKKPEGLSFEEASCFGITYTTAWNSLAS
                     RAGIGQGDTVLIHAAGSGVGTALIQVAKLYHATVITTVGDDWKAEKAKELGADFVINY
                     RKGDFAAEVKRITKGELADIAVDHIGTATFNKSLSCLKRGGRLVTFGATSGDEVSLSL
                     RYIFGKNIQIHGIYVGPKAAMARYLKLAPQKLRPVIDSVFEFGDAQKAYEKLLSRQFF
                     GKIVVRNPQA"
     gene            complement(1915..4401)
                     /locus_tag="VFK23_11335"
     CDS             complement(1915..4401)
                     /locus_tag="VFK23_11335"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HET7319720.1"
                     /translation="MAANHNKLGKFEFDNLGNDRASLRRSISNRLIYSIGKDPITATH
                     RDWFHTTAYAVRERLIELWMETMRSYYNADVKRVYYLSMEFLTGRLLVNSLLNMEFYE
                     ECKGALADLELDLDRIRELEADAALGNGGLGRLAACFLDSMATLRLPGYGYGIRYEYG
                     MFTQRIENGHQIEHPDNWLRYGNPWEFPRPEVLFPVKFGGRVVQVRDEEGHTRYHWID
                     TEDVMAMAFDTPVPGYDTTTVNNMRLWSAKASRDFNLKYFNEGNYIKAVEEKNESENL
                     SKVLYPDDTTQMGRELRLKQQYFFVAASLQDILRRFTKYHPDLAELPDKVAIQLNDTH
                     PSIGIPELMRILLDLYRMDWDRAWDITRRTFSYTNHTLMPEALETWPVSLFEVLLPRH
                     LQIIYEINYRFLEEVRHRCPGDTDILRRMSIVDETGGRRLRMAHLAIIGSHKVNGVSQ
                     IHTELMKTTIFADFDRLSPEKIINVTNGVTQRRWLHLADPGLSSLITKHIGSGWLCDL
                     SKLKGLIPLSKDAAFRAQFRAVKHANKERLAEIIMMRLKLAVDPASLFDVQIKRIHEY
                     KRQLLNVLHIVTRYNRIREGRAENAVPRTVIFGGKAAPGYAMAKLIIKLIHSVADVVN
                     NDPKVGDLLKVVFIPDYNVSNAEKIIPACDLSEQISTAGTEASGTGNMKLGLNGALTI
                     GTLDGANIEMREEVGEENIFIFGLTAAEAAALRAKGYNPWDIYNSNAELRQTLDMIGG
                     GFYSPDEPGRFRPIFDALTGYGDHYLLLADYAAFVSCQERVDALYRDPEEWSRKAILN
                     VAGMGRFSSDRSIREYAERIWKVTSMPERT"
     gene            4738..5541
                     /locus_tag="VFK23_11340"
     CDS             4738..5541
                     /locus_tag="VFK23_11340"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7319721.1"
                     /translation="MKIEVSKGVNLFVQDWGRGKPIVFVHGWPYGHRIFEYQMMQLAK
                     KGYRAVGIDLRGYGYSERPWEGNDYDTWASDIGRVIKSLNLKDVTLFGYCLGGAVALH
                     YWASTRDPRVTKLALASTALPWAGQKPEDRKWYDEHIKYCLEDQVKCIWDFTANVFHK
                     PVSAEYNRWLAHLGDIASVYAVVRGLEEMRDRDLGKEVGKITIPTRIYHGVHDKVLSY
                     TWAESLQRLIKGSVYIRFENSGHALYWDERDKLADELEKFVAESLAKAA"
     gene            complement(6036..7019)
                     /locus_tag="VFK23_11345"
     CDS             complement(6036..7019)
                     /locus_tag="VFK23_11345"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HET7319722.1"
                     /translation="MPTPYQPIHLRGLVIDPPLLLAPMAGLTHSALRQIMQGFGGVGL
                     LSTEMLSARSLPAENPRVSPYLVRTASEKLLSYQLLTNSAGELGPAIDKLHEMKADAV
                     DLNMGCPAPAVGRIGAGIRLMEQPDEVRRIVSEARKRTPLPLTAKIRIGTGLDDGKLL
                     AFCMMLEEEGVDMLSVHARLKHESFARKPRWESIRRVKERLTIPVIANGGIFSVEDAR
                     QCLRVSGADGLMLGRGAVIRPWLFADISREVFGSPVAVSDVCLPAVYSRYVELLCGLF
                     RPEYRLGRLKQFTHYFAQNYKFGHHLASRVQSSASIDEARERAALFFETCA"
     gene            complement(7024..7368)
                     /locus_tag="VFK23_11350"
     CDS             complement(7024..7368)
                     /locus_tag="VFK23_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HET7319723.1"
                     /translation="MKTIEQDGLTINVDEDGFLLKPGDWTEQVARVLAALEGIPDLPE
                     HRLDILKFMREYYRKHNFFPIVRYVCRNVRQPDNCVTEQFIDPVKAWKIAGLPNPGED
                     VIMFKAWEPLGY"
BASE COUNT         1535 a         2065 c         2222 g         1594 t
ORIGIN      
        1 cgtcaagaac tgtctcacgg aaaatcatag cgttatgatg aatagacagt ggcacttctt
       61 ttgcaaaaaa tatattactt ctatatttcc atttaatttt gtacaaacag ataaacagat
      121 agtattaccc gatattacgc atgaacagct tctggctctt gtattttgaa agacgccggt
      181 caagcataat atttttcccc cgagcaagca cacgccggca tgtaagcggg aagtacaatt
      241 ggcagcaaaa acctgatgac aatggagaaa ggagcgatct tatgaacgcc ggcggagata
      301 aaggggaaaa aacgatcctg ctggtcgacg atgataaagg aaacagaaca atcttcgcgg
      361 agatactgac cgacatgggg tacatggtca tcgacaagcc tgatgtcact tccgcgttgg
      421 aggtcgttcg caacgagatg cccatcgacc tcgtgatcac ggattatcgc cttcccgata
      481 cggacggctt cacactcgtg gacgccgtgc gtaaagcgcg gcccggcgtt cagatcatca
      541 tgatgaccgc atatggaaac gcggaaaact acatcaggtc catgagcctc ggggtcttcg
      601 agtatctcaa caagcccgtg aagaaaaaag agttcgaacg aatcgtcagc ttcgctctca
      661 gcaagtccgc cagtgcctga cagattgctg aaaaaccgta tctaagcctg cggattcctc
      721 accacgatct tcccgaagaa ctgccgtgag aggagcttct cgtaggcttt ctgcgcgtct
      781 ccaaactcga aaacggaatc gataaccggc ctgagtttct gcggggcaag cttgagatag
      841 cgtgccatcg cggccttcgg tcccacgtag atgccgtgaa tctgaatgtt cttcccgaag
      901 atataccgca aacttaacga aacttcgtcg cccgacgttg cgccgaaggt cacgagcctg
      961 ccgccgcgtt tgaggcagga cagggactta ttgaaggtgg ccgtcccgat gtggtccacg
     1021 gcgatgtccg caagttcccc cttcgtgatc cgcttcacct cagcggcgaa gtctcccttc
     1081 cggtagttga tgacgaagtc cgcgccgagt tctttcgcct tttccgcctt ccagtcatct
     1141 ccaacggtcg tgatgaccgt ggcgtgatag agcttcgcca cctggatcag ggccgtgccc
     1201 acaccgcttc ccgccgcgtg gataaggacc gtgtcgccct gcccgatgcc cgcgcgggag
     1261 gcaagagagt tccatgccgt ggtgtaggtt atcccgaaac aggaggcttc ctcgaaggag
     1321 aggccttccg gctttttgaa gatgttatct cccggcgcct tgacgtactc ggcagacacg
     1381 ccctgcgccg cgacaccgat aatggaaaag ccggcgcatt cattatcgcg gccgctgagg
     1441 caccgctcgc aatggccgca ggaaagaccg ggaaacagga tcacctcgtc gccttgtttg
     1501 acgtgatgga cgccggctcc ggtcttttca accacgcccg ccgcgtcgga ggcgaagacg
     1561 tggggaaggg ggatcttgac cggatagtcg ccgcccatga cccagacgtc gagatggttc
     1621 agggagaagg ccttgaccct gaccaggacc tcgctttcgg ttatctccgg cacgggaaac
     1681 tcgccggtct tgatcccctg gtatcccttc tgttcgtcga agtaaagagc cttcataatg
     1741 cctcctgtgc cccgtaaccg tatgcaaaaa tctttcatca agacaaaaac ttcttagccg
     1801 caaaaaggcg caagaagcac aagaagacct taaaaacttt tgtgcattct gcgcatcttt
     1861 gcggcaaata cgaatttaag attttctctg tgtcctctgt ggcgaaaaat tttttcaggt
     1921 cctctccggc atcgatgtga ctttccagat acgctcggcg tattcgcgga tcgaacgatc
     1981 gctcgagaac ctgcccatgc ccgcgacgtt caggatcgct ttccgggacc actcctcggg
     2041 atcgcggtag agcgcgtcca cccgttcctg gcaggagaca aaggccgcat agtcggcaag
     2101 aagaaggtaa tggtccccgt atccggtgag ggcgtcgaag atcgggcgga accttcccgg
     2161 ctcatcgggc gagtagaagc cgccgccgat catgtccagc gtttgccgga gctcggcatt
     2221 ggaattgtag atgtcccagg ggttgtatcc ttttgcccgg agcgcggcgg cctctgccgc
     2281 ggtcaggccg aagatgaaga tgttttcctc gcccacttcc tctctcatct cgatgttcgc
     2341 gccgtccagc gtgcctatgg tcagggcccc gttcagtccg agcttcatgt tgcccgtgcc
     2401 cgaggcctcg gtgcccgccg tggagatctg ctcggaaagg tcgcaggcgg ggatgatctt
     2461 ctcggcgttc gaaacgttgt aatcggggat aaaaacgacc ttgagcagat cgcccacctt
     2521 cgggtcgttg ttcaccacgt cggcgacgct gtggatgagc ttgatgatga gcttggccat
     2581 ggcgtagccg ggggcggcct tgcctccgaa gatcacggtg cgcgggacgg cgttttcagc
     2641 ccggccttcc cggatgcggt tgtaccgcgt cacgatgtgc agcacgttca aaagctgccg
     2701 cttgtattca tggatgcgct tgatctgcac gtcgaagagt gaggcgggat cgacggcgag
     2761 cttgagcctc atcatgatga tctcggccag ccgctccttg ttcgcatgct tcacggcccg
     2821 gaactgcgca cggaacgcgg cgtccttcga taggggaatg agccccttca gcttcgagag
     2881 gtcgcagagc catccactgc cgatgtgttt cgtgatgagg gacgaaagcc cgggatcggc
     2941 caggtggagc cagcggcgct gcgtgacgcc gttggtgacg ttgatgatct tctcggggga
     3001 gagccggtcg aagtcggcga agatggtcgt cttcatgagc tcggtgtgga tctgcgacac
     3061 gccgtttacc ttgtggcttc caatgatggc caggtgggcc atgcgcaggc gccggccgcc
     3121 cgtttcgtcc acgatcgaca tgcgccgcag gatgtccgtg tctccgggac agcggtggcg
     3181 cacctcttca aggaaccggt aattgatctc gtagatgatc tgcaggtgcc gcggcaggag
     3241 cacctcgaag agggagacgg gccaggtctc cagtgcctcg ggcatgagcg tgtggttcgt
     3301 gtaagagaag gtgcggcgcg tgatgtccca ggcccggtcc cagtccatgc ggtagaggtc
     3361 caggaggatg cgcatgagct cggggatgcc gatcgacggg tgcgtgtcgt tcaactggat
     3421 cgcgaccttg tccggcagct cggcaaggtc cggatggtac ttggtgaagc gccgcagaat
     3481 gtcctggagc gaggcggcca cgaagaagta ctgctgtttg agcctgagct cccggcccat
     3541 ctgtgtggtg tcgtcggggt agagaacctt gctgaggttc tccgactcgt tcttttcctc
     3601 gacggccttg atgtagttcc cttcgttgaa gtacttgaga ttgaagtcgc gcgacgcctt
     3661 ggcggaccag agccgcatgt tgttcacggt ggtcgtatcg tagcccggca cgggcgtgtc
     3721 gaaggccatg gccatcacgt cttcggtgtc tatccagtga tagcgggtat gtccctcctc
     3781 gtccctgacc tgcaccacgc ggcctccgaa ctttaccggg aagagcacct cgggccgggg
     3841 gaattcccag gggttgccgt accggagcca gttgtcgggg tgctcgatct ggtgcccgtt
     3901 ctcgatcctc tgggtgaaca tgccgtattc gtaccggatg ccgtacccgt agccgggcag
     3961 gcgcagcgtc gccatcgaat cgagaaagca cgcagcaagc cttcccagac ccccgttgcc
     4021 cagggccgcg tcggcctcga gctcgcggat gcggtcgagg tcgagctcca ggtcggcgag
     4081 cgcccctttg cattcctcgt agaattccat attgaggagc gagttcacca ggaggcggcc
     4141 ggtcagaaac tccatggaaa ggtagtagac gcgcttgacg tcggcgttat agtagctccg
     4201 catggtctcc atccagagct cgatcagccg ttcgcgaacg gcgtaggcgg tggtgtggaa
     4261 ccagtcgcgg tgcgtggcag tgatcgggtc cttgccgatg gaatagatca gccggttgga
     4321 tatcgaccgc ctgagagagg cccggtcgtt gccgagattg tcgaattcaa atttgcctaa
     4381 tttattatga ttggcggcca tagcgcctcc ggaagagaat actttgttat tttacctgaa
     4441 aaaacgcatg ggcgtcgttt tttgtcgtcg tattcggggt aataattgtg tttcgattcc
     4501 gccggcttca gagtacttta gaatgggacc cagctcagtc aaattataat cctgaattgc
     4561 tactttcacc gaattgacat aggcgccatg atggcaggac aatataagta gaagaagata
     4621 ttaaggaatg gagcttatct gtaacagaaa taacaaaagg gcttgactcg cgcaaataag
     4681 ccgcggccat cgttttttta tgcgcggtgc atacgatcag gccgaaggag gtatgtgatg
     4741 aagatagaag tatcgaaagg agtgaacctg ttcgttcagg actggggcag gggcaaaccg
     4801 atcgtcttcg tacacgggtg gccttacggc cataggatat tcgagtacca gatgatgcag
     4861 ctcgcgaaga agggctaccg ggcggtcggc attgacctgc ggggctacgg ctattcggaa
     4921 aggccgtggg aaggaaatga ctacgacacg tgggcgagcg acatcgggcg ggtcatcaag
     4981 agcctgaacc tgaaggacgt cacgctgttc ggctactgcc tgggcggcgc ggtcgctctg
     5041 cactactggg cctcgaccag ggacccgcgg gtgacgaaac tggcgctcgc ttcaaccgcg
     5101 ctcccgtggg cggggcagaa gcccgaagac aggaagtggt atgatgagca catcaaatac
     5161 tgcctcgaag accaggtgaa atgcatctgg gactttacgg cgaatgtgtt ccataagccc
     5221 gtaagcgcgg agtacaaccg ctggctcgct cacctgggtg acattgcgtc ggtctacgcc
     5281 gttgttcgcg ggctcgagga aatgagggac cgcgacctgg gcaaggaggt gggcaagatc
     5341 accataccga ccaggatcta ccacggcgtg cacgacaagg tgctttccta cacctgggcc
     5401 gaatcgctgc aaagactgat caaaggatcg gtctacatcc ggttcgagaa cagcggccat
     5461 gccctctact gggatgaaag ggacaaactc gccgatgaac tggagaagtt cgttgccgag
     5521 tccctggcca aggccgcata acaagcagaa cacatcagcc ggcgcttccg tagttcaagc
     5581 gccggcttga gttttgtaaa atcaatttgt cattcctgcg aaagcaggaa tctgtttttt
     5641 aggatattct aggttttgtg tgggtagttt ttcacccttt atcgattttc ttgcatatcc
     5701 ccttcgcagc ctgaacgaaa tttttgaccc gtctaaggcc acgtcagctc gatcgcgcaa
     5761 actcggccga cggcctcaga cgccctgcac tcgtgctgct gcactcgtaa acgcgggtgc
     5821 aggcagtgca tcgcgctcgc ccttgcggga cgcttcctta ggccaagacg ggtgccccaa
     5881 aaatttcata gggccgctcc ggggacatgc ggggttggtt taaagacgtt tcaccaaagc
     5941 ttcccatgcg aaaatccagg catgcgaccg agcccgaggg ttaaacattt cggactgtct
     6001 gagcccgaag ggcgagtttc tgaaatgttt gttcgtcatg cgcaggtttc aaaaaagaga
     6061 gcggcccttt cacgcgcttc gtctatgctc gcgctcgact gcacgcggga ggccaggtga
     6121 tggccgaact tgtagttttg ggcgaagtaa tgggtaaatt gcttgagtct ccccagccgg
     6181 tattcaggcc ggaacagtcc gcagagaagt tccacgtacc ggctgtagac cgcgggcagg
     6241 cagacatcgg acaccgcaac cggagagccg aacacctccc tgctgatgtc agcgaagagc
     6301 caggggcgga tgaccgcccc gcggcccagc atgagcccgt ctgcgccgga gacccgcagg
     6361 cactgccggg cgtcctcgac ggagaagatc ccgccgttgg cgatgacggg gatcgtgagc
     6421 cgttccttca cccgtcgaat gctttcccat cgcggctttc tggcaaagga ttcgtgcttg
     6481 agccgggcat ggaccgaaag catatcgacg ccctcctctt cgagcatcat gcagaaggcc
     6541 agcagtttcc cgtcgtccag gccggtgccg atccggatct tggcggtcaa ggggagcggg
     6601 gtgcgcttcc gcgcctcgga cacgatgcgc cggacctcgt ccggctgctc catcagcctg
     6661 atgcccgcgc cgatccttcc caccgcgggg gccgggcagc ccatgttgag atcgacggca
     6721 tcggccttca tctcatgcag cttgtcgatg gccgggccaa gctctcccgc gctgttggtc
     6781 aggagctgat aggagagaag cttttcggag gccgtcctta cgagataggg ggagacccgg
     6841 ggattttccg cgggcaggct ccttgccgac agcatctccg tcgacaggag gcccaccccg
     6901 ccgaagccct gcatgatctg ccggagtgcg ctgtgggtca ggcctgccat gggggcaagg
     6961 agcaggggcg gatcgatgac gagaccgcga agatggatgg gttgataagg agtcggcatt
     7021 ccgctaatac ccgagcggtt cccaggcctt gaacatgatg acgtcctcgc cggggttcgg
     7081 caggccggcg atcttccagg ccttcaccgg gtcgataaac tgctccgtca cacagttgtc
     7141 gggctggcgg acattcctgc acacatagcg cacgatgggg aaaaaattgt gtttccggta
     7201 atattctctc atgaacttca ggatatcgag ccggtgctca ggcaggtccg ggatgccttc
     7261 cagcgcggcc agaacgcgcg caacctgttc cgtccagtcc cctggtttta acaagaatcc
     7321 gtcttcatcg acatttattg taagcccgtc ctgctctatc gttttcatgg tgttctcctc
     7381 ttaggctcac cggaggtctt cgtagttttt tgcggg
//
LOCUS       DASSLD010000627         4444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1603363, whole genome shotgun sequence.
ACCESSION   DASSLD010000627 DASSLD010000000
VERSION     DASSLD010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603363"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1069)
                     /gene="gap"
                     /locus_tag="VFK23_11355"
     CDS             complement(62..1069)
                     /gene="gap"
                     /locus_tag="VFK23_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET7319724.1"
                     /translation="MAVRVAINGFGRIGRNVFRASQVNKDIEIVAINDLTDTKTLAHL
                     LKWDSTFGEFPHDVKAGEGKLVVGGKDIAVLAKKDPAELPWKDLKIDIALECTGLFLD
                     KASAGKHITAGAKKVLISAPAKDPDATFVIGVNEHTYDPAKHHIISNASCTTNCLAPM
                     TKVVHENFGIVHGLMNTIHSYTNDQQLLDLPHKDLRRARAASVSIIPTTTGAAKAVSL
                     VLPELKGKLDGMSLRVPTPDVSVVDVVFEVSKKTTKEEVNAALKSAAEGKLKGILQYE
                     EAPLVSIDFKGNPHSSIIDAALTGVMEGTMVKVISWYDNEWGYSNRLRDLAILVAKKG
                     L"
     gene            complement(1199..2524)
                     /locus_tag="VFK23_11360"
     CDS             complement(1199..2524)
                     /locus_tag="VFK23_11360"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HET7319725.1"
                     /translation="MAKIIPFRGVVYNQSKAGAIADLVCPPYDIISHEAQQELYRRSP
                     YNVIRLEYGLESPGDTEADNRYTRAAALLDEWFRKDVLRQGQDPAFYVYEMEYKVDGS
                     KKRLRGFVCLVRVEDYDSGMVKPHETTLSGPKTDRLNLLRACRACFSQIFSLFSDPQG
                     SVAAILGKLRGTPEMEVNNSDGVIHRIWTLRGKDDLEVVAREMADKPFFIADGHHRYD
                     TALNYRNERRKAAGTFTGEEGYNYTAMFLARLEDPGLTILPAHRALFNLTDFQPRRFE
                     DDLGRYFNIERIDFSKKSEAKDRRTVLDTMTHRADHAHVFGMRVKGEHSYYLLTLRDV
                     KDLDALLPDKSPAYRRLDVSILHHLIIDRLLGVRMETHKLGLNIEYIKEAEEADRRVH
                     EGLADIVFFMNPTKVREVKDVAEAGERMPQKATYFYPKLLTGLVMHKID"
     gene            complement(2785..2976)
                     /locus_tag="VFK23_11365"
     CDS             complement(2785..2976)
                     /locus_tag="VFK23_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HET7319726.1"
                     /translation="MKMREIREIAKKKGVTGAKKMDKTSLIRAIQKAEGNNPCFSTKP
                     LNECDQMNCLWREDCTIAV"
     gene            complement(3022..3621)
                     /locus_tag="VFK23_11370"
     CDS             complement(3022..3621)
                     /locus_tag="VFK23_11370"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET7319727.1"
                     /translation="METPVSALLLAAGSSSRMGQIKQLLKLGDKTVIRRCVDTLVGGG
                     VNEILVVIAACRGGLAPHLRGLPVSIAVNETPYSDMAESFRVGLKQLRHYTSSVLVCL
                     SDHPLVSAETVRSILREHRAFPGKIIVPEYDGRRGHPSLFPRNILDEIFTGATLRDIV
                     RKDPSRVKPVTVGDEGVVLDMDTRQDYEMIVKRFQKKAR"
     gene            complement(3536..4378)
                     /locus_tag="VFK23_11375"
     CDS             complement(3536..4378)
                     /locus_tag="VFK23_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HET7319728.1"
                     /translation="MQIYEEVLRLKRLGRTSAIATIVECRGSAPQKQGAKMLVRDDGS
                     LLGTLGGGCLEADVVQAALMAMKDGSPLTLPFELTEREGGLVCGGSVLVYIEPVVQEP
                     SLVILGAGHVGRTLARLARFTGFRVTVVDDRPEFANPENVPDASDIVVHPFERVFDRV
                     PVDRKTFIVVATRGHNHDLDAVRAALGTDAHYIGLLGSRRKKGLLFQALTGSGFSKED
                     VARIIIPVGAPIGSVTPEEIAVSIMAQIIEKRRQHGDARLGPAACGRIVQQDGSDQTT
                     PQAR"
BASE COUNT          812 a         1301 c         1333 g          998 t
ORIGIN      
        1 cactaatatc gaaattctaa acctcgttta ggatttagga tttcgtactt cggatttgca
       61 gttagagtcc tttcttcgcc accaggatcg ccaggtcgcg gaggcggttc gaatagcccc
      121 attcgttgtc gtaccaggaa atcaccttca ccatcgtgcc ttccatgacg cccgtcaagg
      181 ccgcgtctat gatggaggaa tgggggttgc ccttgaagtc gatggacacg agcggcgcct
      241 cctcgtactg gaggatgccc ttgagcttgc cctcggcggc ggacttgagc gcggcgttga
      301 cctcttcctt ggtggtcttc ttcgagacct cgaagactac gtcaacgacc gacacgtccg
      361 gcgtgggcac gcggagcgac atgccgtcga gcttgccctt gagctcgggc agcacgagcg
      421 acacggcctt ggccgcgccc gtagtcgtcg ggatgatgga cacgctggct gcccgtgccc
      481 ggcgcagatc cttgtgcggc aggtcgagga gctgctggtc gttggtgtag gaatggatcg
      541 tgttcatgag gccgtggacg atgccgaagt tttcatgcac aaccttggtc atgggcgcca
      601 ggcagttcgt ggtgcaggac gcgttcgaga tgatgtggtg tttagccgga tcataggtgt
      661 gctcgttcac gccgatgacg aaggtcgcat cggggtcctt ggccggggcg gagatgagga
      721 ccttcttggc tcccgcggtg atgtgcttgc ccgcgcttgc cttgtccagg aacaggcccg
      781 tgcactccag tgcgatgtcg atcttcaggt ccttccacgg aagttcggcc gggtctttct
      841 tggccaaaac ggcgatgtcc ttgccgccaa cgacaagctt gccttcgccg gccttgacgt
      901 catgcggaaa ctcgccgaac gtggagtccc acttcagcag gtgcgcaagc gtctttgtgt
      961 ccgtgaggtc gttgatcgcc acgatctcaa tgtccttgtt gacctgcgaa gcgcggaaca
     1021 cgttcctgcc gatgcgtccg aatccgttga ttgcgactct tacagccatg gtagcctcct
     1081 tgatttaaag ttcagagcgc tgaaatgaca gttcaaagtt caaggttcaa agttcaaagt
     1141 caacaattac gatccgcggc atccactctg gactctgaac tgagaactct gaactttttt
     1201 aatctatttt atgcatcacc agccccgtca ggagcttggg gtagaagtac gtcgcctttt
     1261 gaggcatgcg ctcgccggcc tccgccacgt ccttcacctc ccgcaccttc gtggggttca
     1321 tgaagaagac aatatcggca aggccctcgt ggaccctccg gtccgcctct tcggcctcct
     1381 tgatgtattc gatgttgagg ccgagcttgt gggtctccat cctgacgccc aggagccggt
     1441 cgatgatcaa atggtgcagg attgagacgt ccagcctccg gtacgccggt gatttgtccg
     1501 ggagcagcgc atccaggtcc ttcacgtccc ggagggtcaa aaggtaatag ctgtgttcgc
     1561 ccttgacccg catgccgaag acgtgggcat ggtcggcccg gtgcgtcatg gtgtcgagga
     1621 ccgtgcgcct gtctttggcc tcggactttt tactgaaatc gatgcgctca atgttaaaat
     1681 agcggcccag gtcgtcctcg aagcgccgcg gttgaaagtc cgtcaggttg aagagcgcgc
     1741 ggtgcgccgg gaggatggtt agtcccgggt cttcgagccg ggccaggaac atggccgtat
     1801 agttgtagcc ttcctcgccg gtgaacgtgc ccgccgcctt gcgccgctcg ttccggtaat
     1861 tgagcgccgt gtcgtagcgg tgatgcccgt cggcgatgaa aaagggcttg tccgccattt
     1921 cgcgggccac gacctcgagg tcgtccttcc cgcgcagcgt ccagatgcgg tgaatgacgc
     1981 cgtcgctgtt attcacctcc atctcgggcg tgcctcggag tttgccgagg attgcagcga
     2041 ccgatccctg cgggtccgag aagagcgaaa atatctggct gaagcaggcc ctgcaggcgc
     2101 gcaggaggtt gagccggtcg gtcttgggcc ccgagagcgt cgtctcatgg ggcttgacca
     2161 tccccgagtc gtagtcctcg accctgacga ggcagacgaa gccgcggagc ctttttttcg
     2221 acccgtcgac cttatactcc atttcataga cgtagaaggc cgggtcttgc ccctgccgca
     2281 ggacgtcttt cctgaaccac tcatccagga gcgcggccgc gcgggtgtag cgattgtccg
     2341 cctccgtgtc gccggggctc tcaagaccgt attccagccg gatgacgttg taggggcttc
     2401 tccggtacag ctcctgctgg gcctcgtgcg agatgatatc atagggcgga cagacgagat
     2461 cggcgatggc gcccgctttt gactggttgt acacgacccc ccggaagggg atgatcttcg
     2521 ccatggattg cgtccttgcc gcccgcgtga agcccgggga aaagcctctc aaaagacttc
     2581 ttttatagca cagccttcat tttattgtca atatattttg cagtcgggct aagtacttag
     2641 atgatctaac aggggagaaa attgccgcaa aaaggcgcaa gattcgcaag aatttaatgg
     2701 attttttgcg atttttgcgc atctttgcgg agaaataatt ttgggtccgg tttgtccggg
     2761 ctacgccctg ggaagagaag aggactaaac cgctattgtg cagtcttcgc gccaaaggca
     2821 gttcatctga tcgcattcgt tcaggggctt ggttgagaaa caggggttgt tcccctccgc
     2881 tttctggatc gccctgatca gcgatgtctt atccattttt ttcgcgcccg tgaccccctt
     2941 ttttttcgca atttccctga tttcccgcat tttcatgatg aagccctcct cgtatggaat
     3001 gagattattt tccctatgag cttaccgtgc ctttttttga aatcgtttca cgatcatttc
     3061 gtagtcctgt cgcgtgtcca tgtcgaggac cacgccttcg tccccgacgg tcacgggctt
     3121 caccctggac gggtccttcc ggacgatatc gcggagggta gcgccggtaa agatttcatc
     3181 gaggatgttt ctcgggaaga ggcttgggtg gccccgcctg ccgtcatatt cagggacgat
     3241 gatcttgccc ggaaacgccc ggtgttcccg aaggatggac ctgacggttt ccgcggacac
     3301 gaggggatgg tcggacaggc agaccaggac ggaagaggta tagtggcgaa gctgtttcag
     3361 accaacccgg aacgattccg ccatgtcgct gtacggcgtt tcattcaccg caatggacac
     3421 cggaagtcct cgcaaatgag gagcaagccc gcccctgcat gcggcgatga cgaccaggat
     3481 ctcgttcacg cccccgccca cgagggtgtc gacgcagcgt ctgatgaccg tcttgtcacc
     3541 gagcttgagg agttgtttga tctgacccat cctgctggac gatcctgccg caagcagcag
     3601 ggccgagacg ggcgtctcca tgctgtctcc ttttttcgat gatctgcgcc atgatgctca
     3661 cggcgatttc ctcgggtgtc accgagccga tgggcgcccc cacggggatg atgatcctgg
     3721 cgacgtcttc ctttgaaaat ccactgcctg tcagggcctg gaacagcagg cctttcttgc
     3781 gcctgctgcc gagtaggccg atgtagtgcg catcggtccc aagcgcggcc ctgaccgcgt
     3841 cgaggtcgtg gttatgcccg cgcgtcgcca cgacgatgaa ggtcttcctg tcaacgggca
     3901 cccggtcgaa gacgcgctcg aacggatgga ccacgatgtc gctggcatcg gggacgttct
     3961 cgggattcgc gaattccggg cggtcgtcca ccaccgtcac gcggaaaccc gtgaaccggg
     4021 cgagcctggc gagggtcctg cccacgtggc cagccccgag gatcacgagg ctgggctcct
     4081 gcacgaccgg ctcgatgtaa acgagcacgc tgccgccgca gacgagtccg ccctcgcgct
     4141 ccgtaagctc gaagggcagg gtcagggggg agccgtcctt catggccatg agcgccgcct
     4201 gcacgacgtc ggcctcgaga cagccgcctc ccagggtccc cagcagggag ccgtcgtcgc
     4261 ggacgagcat tttagcgccc tgcttctggg gcgccgaccc gcggcactcg acgatggttg
     4321 cgatggcgga ggtcctgccc agacgcttga gcctgaggac ttcttcgtaa atctgcatgc
     4381 tttcccccat aaacagcaac aaaacccaaa ccgtgcaaac cggaaccaag gacagtcgcg
     4441 cgca
//
LOCUS       DASSLD010000628         3031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_568350,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000628 DASSLD010000000
VERSION     DASSLD010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568350"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..1691)
                     /locus_tag="VFK23_11380"
     CDS             complement(216..1691)
                     /locus_tag="VFK23_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(A) polymerase"
                     /protein_id="HET7319729.1"
                     /translation="MSPIQPAIIRRSEHNISRKWISHNALRVLYRLKEKGYYAYLVGG
                     GVRDLLLGREPKDFDIATDARPGEVKKAFRNCRLIGRRFRLAHVHFHDEIIEVATFRS
                     NITEEPEAPEATPAVQPGPPEAAESRLPEPGLAPGAQQQATAAPEGPAAKPRPPRLLK
                     TEDGMILRDNVFGTPEEDALRRDFTVNALFYNIADFSVIDYVGGMEDLKKGLIRTIGD
                     PAVRFTEDPVRMVRAVRFAAMLGFAVEENTYRAMMDLRDKVALASPARMYEEVMKLFL
                     LGEGEKTYQLLRKTGLFGVIFPRLNEWVDTENDGFPHTWIGKALEWVDTCVQAGRKVE
                     PHILFGLMYGQFIEEKAAEAVKAGLPSLEALTKAVSETMSEQAQRVMIPRKVGLAMRD
                     MYWNQHRFEKREGRHPRYFLNRPGFTDAFEYLRFRSEATGEKSELRAWWKEFVKANPA
                     TGGEAPREGKGRREPGGKDRARPPRRRRRGRNPKSQKPNPK"
     gene            complement(1747..>3031)
                     /locus_tag="VFK23_11385"
     CDS             complement(1747..>3031)
                     /locus_tag="VFK23_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET7319730.1"
                     /translation="TVYSGEEQAGLLKSLLSCSAKKAGEAAERISRIKNFVEEGDEEV
                     RALLASYQAALKEKNAVDFDDLILRPIEILTNNPFAFKSSFTHIMVDEYQDVSPAQYA
                     LLRLLAQNGARLCAIGDSDQAIYGFRGADAGAFLRFGDDFRNAASITLARNYRSTETV
                     VYASSAVIRNNRLRIEKEIASTREGGGPVGVISVADERAEGKWIVREIEARIGGTSHD
                     QMRKAGRLKDATEGTFTFSDFAVIVRTNAQAKAIEEAFIGSGIPYQVIGRKSSLQSAA
                     LEETVAFLRSLALPDDAASGPVVGNEAKLLAPADYFDPRANAVTLTTMHTSKGLEFRV
                     VFIAGCEDGLIPCTIMKDAIDIEEERRLFYVGMTRAKDELFLLHARSRFLYGQRLAPP
                     PSPFLEEIPGELTRRTVVPEKVKKQRESDRQMGLF"
BASE COUNT          557 a          926 c          888 g          660 t
ORIGIN      
        1 ttcatgcgaa gcacggtcgc gatgtgcctc acgtcctctc cggtgatcgt gatggcaggc
       61 ccggaaatct ggtcagggga tatgaaaaat cgcgggggtc tggacatgct gtccttcaga
      121 gtcgcaaatt ccaaacatca agcatcaaat ctcaaataaa ctccaaaaaa caattttcaa
      181 acacttttgg tcattcatat tttgaatttg aatattcatt tgggatttgg tttttgagat
      241 ttgggatttc tccctctccg cctgcggcgg ggcggccgcg cgcggtcctt cccgccgggt
      301 tccctgcgcc ccttcccttc gcgtggtgct tcaccgcccg tagcgggatt agccttgacg
      361 aactccttcc accaggcgcg gagttcgctc ttctcgcccg tggcctcgct cctgaaccgg
      421 aggtactcga aggcatcggt gaaaccgggc cggttcagga aataccgtgg gtgccttccc
      481 tcccgttttt cgaaccggtg ctggttccag tacatgtcgc gcatggccag cccgaccttc
      541 cgggggatca tcacgcgctg cgcctgctcg gacatcgtct cggagaccgc cttcgtgagg
      601 gcctcgagcg aaggcaaccc ggctttcacc gcttccgcgg ctttttcctc gatgaactgt
      661 ccgtacatca ggccgaaaag gatatgcggc tcgaccttcc tcccggcctg aacacaggta
      721 tcgacccact cgagcgcctt gccgatccag gtgtgcggga acccgtcgtt ctcggtgtcc
      781 acccactcgt tcaggcgcgg gaagatcacg ccgaaaaggc cagtttttct gagaagctgg
      841 taggtctttt cgccttcacc gagcaagaag agcttcatga cctcttcgta catcctggcc
      901 ggcgacgcaa gggcgacctt gtcccggagg tccatcatgg cccggtaggt gttctcctcg
      961 acagcgaacc cgagcatggc ggcaaaccgc acggcgcgca ccatgcgcac cgggtcctcg
     1021 gtaaaccgca cggccgggtc gcctatggtg cggatgaggc ctttcttgag gtcctccatc
     1081 cccccgacat agtcgatgac cgaaaaatcc gcgatgttgt agaacagggc gttcacggtg
     1141 aagtcgcgcc tgagcgcgtc ctcctcgggc gttccgaaga cgttgtcgcg cagaatcatg
     1201 ccgtcctcgg tcttaaggag gcgcggcggc ctgggtttgg ctgccggacc ttcaggggct
     1261 gcggttgcct gttgttgcgc cccgggggca agccccggtt ccggaaggcg cgattctgcc
     1321 gcctcaggag gccccggctg cacggcaggg gttgcctccg gtgcttcggg ctcctccgtg
     1381 atattggacc ggaacgtcgc cacctcgatg atctcatcgt ggaaatggac gtgagcaagg
     1441 cggaatcgcc gcccgatcaa gcggcagttg cggaaggcct tcttgacctc gccgggcctc
     1501 gcgtccgtcg cgatgtcgaa gtccttgggt tcacggccca ggagaaggtc gcgcacaccg
     1561 cctcccacga ggtaggcgta atagcccttc tccttcaggc gatagagaac gcggagagcg
     1621 ttgtgactga tccacttgcg ggagatgttg tgttcggagc ggcggatgat cgcaggttga
     1681 ataggactca cgggattgta tattagcaca ccggggtggg taaggaaaga gggaaaaggg
     1741 catgcttcaa aacaagccca tctgcctgtc cgattctctt tgttttttta ccttctccgg
     1801 cacgaccgtt ctcctcgtca gttctcccgg aatttcctcg aggaacggcg aggggggcgg
     1861 ggccaaccgc tgcccgtaga gaaaacgcga ccgggcgtgt aggagaaaaa gctcgtcctt
     1921 tgcacgcgtc atgccgacat agaagagcct ccgctcttcc tcgatatcga tggcgtcctt
     1981 catgatcgtg caggggataa ggccgtcctc gcatccggcg atgaacacga cccggaactc
     2041 aaggcctttg gacgtgtgca tcgtcgtgag cgtgacagca ttcgcgcggg gatcgaagta
     2101 atcggccggc gcgaggagtt tcgcttcatt gccgacgacc ggacctgagg cggcatcatc
     2161 gggaagggcg agcgacctga ggaaggcaac ggtctcttca agcgccgcgg actggagact
     2221 gctctttctc ccgataacct ggtaggggat cccggacccg ataaaagcct cttcgatggc
     2281 cttcgcctgg gcattcgtcc ggacgatcac ggcaaagtcg gagaaggtga acgttccctc
     2341 ggtcgcgtcc ttcagcctgc cggcctttct catctgatcg tggctcgtgc cgccgatcct
     2401 tgcctcgatc tcccgcacga tccattttcc ctcggcccgc tcgtcagcaa cggagatgac
     2461 gcccaccggc cctcccccct cccgcgtcga ggctatttcc ttctcgatcc tcagacggtt
     2521 gttccggatc acggcgctcg aagcgtacac caccgtctcc gtggaccggt aatttcgcgc
     2581 gagggtgatg gaagcggcgt ttctgaaatc atcgccaaag cgaaggaaag cacctgcatc
     2641 cgcgccgcga aaaccgtaga tcgcctgatc ggaatctccg attgcgcaga gccttgcccc
     2701 gttttgggcg agcagcctga gcagagcata ctgtgcggga ctcacatcct ggtattcatc
     2761 gaccatgatg tgggtgaatg agcttttgaa tgcgaaagga ttgttcgtga ggatctcgat
     2821 gggcctgaga atgaggtcgt cgaaatcaac ggcgttcttc tccttgaggg ctgcctgata
     2881 ggacgcgagg agcgcccgca cctcttcgtc cccttcctcc acgaaatttt ttatccttga
     2941 aattcgttcc gccgcctccc ccgccttttt tgcggaacag ctgagcagac ttttcaggag
     3001 tccggcctgc tcctccccgc tgtacaccgt g
//
LOCUS       DASSLD010000629         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1362566, whole genome shotgun sequence.
ACCESSION   DASSLD010000629 DASSLD010000000
VERSION     DASSLD010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362566"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VFK23_11390"
     CDS             <1..661
                     /locus_tag="VFK23_11390"
                     /inference="COORDINATES: protein motif:HMM:NF014498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HET7319731.1"
                     /translation="MGIAFEFGDEFTLLDMKKKYPGAPVVAYVNTSAAVKAESDICCT
                     SSNVVKVVESLKEDTIICIPDRNLSSYAAKRTKKKIISWDGFCNVHHAHLDVEDVKKA
                     KAEHPDAVLVVHPECPPEVQDMADHITSTSGMLRFCKESNDKEFIIGTEEGILHRLKK
                     DSPDKKFFVLNKEMVCPNMKRTKLDSVLHALEKRQYVIKVPEDIRVRAKRALDRMLEI
                     K"
     gene            783..1916
                     /gene="rmuC"
                     /locus_tag="VFK23_11395"
     CDS             783..1916
                     /gene="rmuC"
                     /locus_tag="VFK23_11395"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET7319732.1"
                     /translation="MNTAILILLVAVVLILVWLVIQSQKKTIDPTIPLLQQEVQSLRG
                     QLTEAMMRNQEAVNQQLGQITMQVNTQLGQATSQMNTRLGQVTEQLQKSTGDLNTRLD
                     NAAKVVSEVSKGLGGLSEATKKVFEVGKDIASLQEILRSPKLRGGLGELFLGDLLAQI
                     FPPAHYTLQHRFKSGEVVDAAIKLGQNLVPIDAKFPLENFRRVVEAATEDERKALKRK
                     FISDVKKHVDAIAVKYILPDEGTFDFALMYIPAENVYYELIIKDEALDAEKGLLNYSF
                     QKRVIPVSPNSFYAYLQTILLGLKGMHVEEQAQEILRNLARLSGDFERFRSDFELLGK
                     HLTNTKTRYDDADKRLAKFGDRLQALSGAESDHHIEETTKLPL"
     gene            complement(1953..>2147)
                     /locus_tag="VFK23_11400"
     CDS             complement(1953..>2147)
                     /locus_tag="VFK23_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET7319733.1"
                     /translation="RAKLTKRVTSHTFRHSFATRLLQANYDIRTIQTLLGHADVRTTM
                     IYTHCVPSKTIKELKSPLDF"
BASE COUNT          513 a          630 c          613 g          391 t
ORIGIN      
        1 catgggcatc gccttcgagt tcggagatga gttcacgctc ctggacatga agaagaaata
       61 ccccggcgcg ccggtcgtgg cctacgtgaa cacctcggcc gctgtcaagg cggagagcga
      121 catctgctgc acgtcgtcga acgtggtcaa agtcgttgaa tcgctcaagg aagacacgat
      181 catctgcatc ccggaccgga acctttcctc ctacgctgcg aaacggacga aaaagaagat
      241 catctcctgg gacggctttt gtaacgtgca ccacgcccac ctggacgtgg aggacgtgaa
      301 aaaggcgaag gccgagcacc ccgacgcagt cctggttgtc cacccggaat gccctcccga
      361 ggtccaggac atggcggacc acatcacgag cacctcgggc atgctgcgct tctgcaagga
      421 atcaaacgat aaggaattca tcatcggcac cgaggaaggg atcctccacc gcctgaagaa
      481 agacagcccc gataagaaat tcttcgtcct gaacaaggaa atggtctgcc ccaacatgaa
      541 acggacgaag ctcgacagcg tgctccatgc cctcgaaaaa cgccagtacg tgatcaaggt
      601 gcccgaagac atccgggtcc gggcaaaaag ggcgctggat cggatgctgg agattaaata
      661 aacgacaggg ggacacagag acacggggac gcggagacgg gcgacaaaag ctaaaatgag
      721 cccatgactg tttttttctc cgcgtcgcgc catcacccct tccccgcgtc ggttcttaaa
      781 caatgaacac cgccatcctc atacttctcg tcgccgtggt cctcatcctc gtctggctcg
      841 ttatccagtc acagaagaag accatcgacc ccaccatccc cctgctccag caggaagtgc
      901 agtccctgcg cggacagctt accgaagcca tgatgaggaa ccaggaagcg gtgaaccagc
      961 aactgggcca gatcacgatg caggtgaaca cccaactcgg ccaggccacg tcccagatga
     1021 acacccggct cggccaggtt acggagcagc tccagaaatc aaccggggac ctgaacacga
     1081 ggctggacaa tgcggcaaag gtcgtctcgg aagtgagcaa gggtctgggc ggcctttcag
     1141 aggccacgaa gaaggtattc gaggtgggga aagacatcgc aagcctgcag gagatcctgc
     1201 ggtcgccgaa gctcaggggc gggctgggcg agcttttttt aggcgatctt ctggcgcaga
     1261 tctttccacc ggcgcactac acgcttcagc accgcttcaa gagcggagag gtcgtggacg
     1321 cggccatcaa gctcggccag aatctcgtgc ccatcgacgc caagttccct ctcgaaaatt
     1381 tcaggcgcgt cgttgaagcg gcgactgagg acgagcgaaa ggccctaaaa aggaagttca
     1441 tcagcgatgt gaagaaacac gtcgatgcca tcgccgtcaa atacatcctc cccgacgagg
     1501 gcaccttcga tttcgccctt atgtacatcc ctgctgagaa cgtctactac gaactcatta
     1561 tcaaggacga ggccttggac gcggaaaaag gccttctcaa ctattccttc cagaagaggg
     1621 tcatccccgt ttcgccgaac agtttctacg cctatctcca gaccattctc ctgggcctca
     1681 agggcatgca tgtcgaggag caggcgcagg agatcctgag aaacctcgcc aggctgtccg
     1741 gagacttcga gcggttccgg tccgacttcg agctgctggg caaacacctg acgaacacga
     1801 agacgaggta cgacgatgcc gacaagaggc tcgcgaagtt cggcgaccgg ctccaggccc
     1861 tgagcggcgc ggaatcggac catcatatcg aagaaacgac gaagctgccg ctgtgaactt
     1921 tttgtgcctg ccgcagttcc tcccttttcc cctcagaaat ccagcgggct tttcaactct
     1981 ttaatggttt tgctcggcac gcagtgggtg tagatcatcg tggtccgcac gtcggcgtgg
     2041 cccagaagcg tctggatcgt tcggatgtcg tagttcgcct gcagaaggcg ggtggcgaaa
     2101 ctgtggcgga aggtgtggga ggtgactcgc tttgtcagtt tggcccg
//
LOCUS       DASSLD010000630         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_167884,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000630 DASSLD010000000
VERSION     DASSLD010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167884"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFK23_11405"
     CDS             <1..531
                     /locus_tag="VFK23_11405"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HET7319734.1"
                     /translation="DAIDGDLVGPDYFPTPMNQAKAVFAKLCILYLELHNSMTLCNYT
                     LPGWASPLKSRNYRGDKDIEAKFFTAVTGESVTRADMEKTGLRILTLFRALTALHMNE
                     KDQRNKHDIMPEWAFHPHGASSATLDRTDWEVAKDMLYAEFGWDKATGLPTQATYDSL
                     GLSDVAARMAAAGLMP"
     gene            608..1507
                     /locus_tag="VFK23_11410"
     CDS             608..1507
                     /locus_tag="VFK23_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319735.1"
                     /translation="MKTVDIEAEAAEETRVEYGAEKAVAAGKTIAEPTVEEIKATIAE
                     KIRADGEERATVTPSEVVFSLVPDSQQGRITAILEEMAADDRYADIKAVTTASGMVFF
                     LSKKHFDTGEAIAKSRLEEVKFLLAEKIRADSKNAVALTSASDLLSLAPEMAQDGITA
                     VLDAMRTDERYADIKSVTSPAGDVYYHSEKHMSGYYAVVLSRVAARDPVATIAAMVRD
                     ESRIYPRPTCIEFFMEPVFNIPPGDLKAVFDEMLQKPEYSDIKLMVHPETGAVYAYSS
                     QYLSGDAAFQKMHYVEVVKPANP"
     gene            1568..>2198
                     /locus_tag="VFK23_11415"
     CDS             1568..>2198
                     /locus_tag="VFK23_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HET7319736.1"
                     /translation="MGITVSYRNGRLEPGETEIVQEFPLKLIVNHREVATLICSPHDL
                     KFLTAGFLRLQGFVRSVEDFLMLSVCNDFGVANVSIRGDVPDRLQPVLTSGCGAGISF
                     TLGQSTKNMPARCDMSQPTYRPSEIFGMMEQLARLAENYKSHGGIHSAAVGDGTSILL
                     YAEDLGRHNTLDRIAGEALLKGIDLSGTMLVTSGRVSTELAAKAAMLGIS"
BASE COUNT          478 a          628 c          683 g          409 t
ORIGIN      
        1 gacgccatag atggcgatct ggtaggccct gattattttc cgactccgat gaatcaggcc
       61 aaggcggtct tcgccaaact gtgcatcctc tacctggagc tgcacaactc gatgaccctg
      121 tgcaactaca ccttgccggg ctgggcctcg ccgctcaaga gccgcaacta ccgcggagat
      181 aaagacatcg aggcgaagtt cttcactgcg gtcacgggcg aatccgtcac gcgggcggac
      241 atggagaaga ccgggttgag gatcctcacc ctcttccggg cgctcacggc ccttcacatg
      301 aacgagaaag accagcgaaa caagcacgac ataatgccgg aatgggcatt ccatccccat
      361 ggcgcgtcct ccgccacgct ggaccgcacc gactgggagg tcgccaagga catgctgtat
      421 gcggagttcg gctgggacaa ggcgaccggt ttgccgaccc aggcgacgta cgacagtctg
      481 ggcctgagcg atgtggcggc cagaatggct gcagcggggc tgatgccata gcgagggaaa
      541 gcccccggcg aaagccgggg gctttccggt ttctggcgga agatcaacga tcgaggggga
      601 tggcaacgtg aaaaccgtcg atattgaagc ggaggccgcc gaggagaccc gggttgagta
      661 tggagcggaa aaggcggtcg cggccgggaa aacgatcgcg gagcctacgg tcgaggagat
      721 aaaggcgacg atcgcggaga agatccgggc cgacggcgaa gagcgcgcga ctgtgacccc
      781 gtcggaggtc gtgttttccc tcgttccaga ttcgcagcag ggcaggatca ccgccattct
      841 ggaagagatg gccgccgacg accgctatgc ggatatcaag gccgtaacca ctgcttcagg
      901 tatggttttt ttcctttcga agaagcattt cgacacgggg gaagcgatcg caaagagccg
      961 gctcgaagag gtgaagttcc tgctcgcgga aaagatccgg gccgactcca agaacgccgt
     1021 cgcgctgacg tccgcctccg accttctttc gctggctccc gagatggcgc aggacgggat
     1081 caccgccgtt ctggacgcga tgcgtaccga cgaaaggtac gccgacatca agtcggtgac
     1141 ctccccggct ggcgatgttt attaccattc ggaaaaacat atgagcggct actacgcggt
     1201 tgtcctgagc cgggtggccg ccagggaccc cgttgccacc atcgccgcca tggtccggga
     1261 cgaatccagg atctacccgc gtccgacctg tatcgagttt ttcatggagc cggtgttcaa
     1321 tattcccccc ggcgacctga aagcggtgtt cgatgagatg ctgcaaaaac cggagtacag
     1381 cgatatcaag ctgatggtcc atcccgaaac gggggctgtc tacgcgtatt ccagccaata
     1441 cttgagcggg gacgcggcat ttcagaagat gcattatgtg gaggttgtaa agcccgcgaa
     1501 tccttagtgc gcaggccggg aaggaagaat cgaacggagc gggatacctg aacggggaac
     1561 gggagccatg ggaataacag tttcctatcg aaacggaagg ctcgagccag gggagacgga
     1621 gattgtccag gaatttccct tgaagctcat cgtcaaccac cgggaggtcg ccaccctgat
     1681 ctgctctccg cacgacctca agtttctgac cgcgggattt ctccgtctcc aaggtttcgt
     1741 ccgaagcgtg gaagattttc ttatgctgag cgtctgcaac gatttcggcg tagccaacgt
     1801 gagcatccgg ggcgatgtgc cggacaggct gcagccggtc ctgacttcgg ggtgcggcgc
     1861 cggtatcagc ttcaccttgg gtcaatcgac aaaaaacatg ccagcgcggt gcgatatgtc
     1921 acaacctacg tatcggccgt ccgagatctt cggcatgatg gagcaactcg cccgactggc
     1981 ggaaaattat aaatcccatg gcggcattca ttcggccgcc gtcggcgacg gcacttccat
     2041 ccttctctat gcggaggacc ttggacggca caataccctg gaccggatcg ccggggaagc
     2101 gcttctgaaa gggattgatc tttccggcac gatgctcgtc acctccggcc gggtttccac
     2161 ggaacttgcg gccaaggcgg caatgctcgg catttcgc
//
LOCUS       DASSLD010000631         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_764545,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000631 DASSLD010000000
VERSION     DASSLD010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764545"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..>2194
                     /gene="glnE"
                     /locus_tag="VFK23_11420"
     CDS             28..>2194
                     /gene="glnE"
                     /locus_tag="VFK23_11420"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HET7319737.1"
                     /translation="MALNHLERFAEALDDVPLFVSVCRTRHEVLRSIITIFGASRFLS
                     TFLIASAADGLACLADPDYLTRPAGKGVLREKLGSLMEGDAEEQAFFRVLRIFRKREL
                     LRIGLRDLLDKADLQETVAELSDLAEVCLQAAYERADAELTGRYGRPLIENPDGSRSP
                     AGFAVIAMGKLGGRELNFSSDVDLMYVYTADGETEGSRSAAAVTANRITNHQYFIKLA
                     EKITAAIGKKTEDGFVFRVDLRLRPEGQGGPLAQSLGGYEIYYESWGQTWERAALIKA
                     RPVAGNDAVGREFLERIAPFVYRKYLDYGAIVEIRDMKQKINKDVEQKGRAHRDVKLG
                     YGGIREIEFVSQALQLLYGGRDRSLRERSTLKALHALSQKGLITYEEHEALAKAYTFL
                     RTVEHRIQILDDLQTQTIPTDEEDLRALARRTGYRERGSEAELLLRDHAGHTPKVRRI
                     YDELFAFTGDESLGSGPAGDYGRLLDPETTEQEAVSLLGRLGFRDPGKAYRNLLLLRE
                     GPAFVHQTPRSRKLFDEIFPALFQEIVASPDPDMALNHLESFLAAQGSWESFQSLVKL
                     DASAFKVLIAVFANSEYLSRMLVGRPVLLQNLLESRRAFGPGVCGRYAKELAGALEKG
                     GDIAEKLDALRRFKHQEEIRLGMADLLNQARQTTVSRDLSKLAEICLGAALGLAAEET
                     GRRYGVSGARGPESGAAGLAIIGMGKLGGRELTYGSDLDI"
BASE COUNT          411 a          681 c          738 g          364 t
ORIGIN      
        1 gagtcggccg cttccgccga tcctgacatg gccctcaatc atctcgagcg gtttgcggag
       61 gccctcgacg atgtcccgct gttcgtatct gtttgccgga cgcgccatga agtcctccgc
      121 agtatcatca ccatcttcgg agcaagcagg tttctgtcca cctttctgat cgcatccgca
      181 gccgacggcc tcgcctgcct cgccgatccc gactacctca cgcgccctgc cggaaagggc
      241 gtactgcgcg agaagctcgg gtccctgatg gaaggcgatg cggaggaaca ggcctttttc
      301 cgcgtcctga gaattttccg aaaacgggag ttgctcagga taggtctgcg ggacctcctc
      361 gacaaggccg acctgcagga gacggtggcg gagctgtcgg accttgccga agtctgcctg
      421 caggcggcct atgagcgggc cgatgcggaa cttacggggc ggtacggcag gccgctgatc
      481 gagaaccccg acgggtcgcg gtccccggcc ggctttgcgg tcatcgccat gggaaaactc
      541 ggcggccgcg agctgaattt cagctccgat gttgacctca tgtacgtcta caccgccgac
      601 ggagagaccg aggggtcgcg cagcgccgca gcggtcacgg cgaaccggat cacgaaccac
      661 cagtatttca tcaagctcgc ggagaagatt accgcggcga tcggcaagaa gaccgaggac
      721 ggcttcgtgt tccgcgtgga cctgcgcctc cgtccggagg ggcagggggg gccgctggcg
      781 cagagcctcg gcggctacga gatctactac gagtcctggg gccagacctg ggaacgcgcg
      841 gcgctcatca aggcgcggcc cgtggccggc aatgatgccg tgggcaggga gtttctggag
      901 cggatcgccc ccttcgtgta ccggaagtac ctcgactacg gcgccattgt cgagatccgc
      961 gacatgaagc agaagatcaa caaggacgtg gaacagaagg ggagggcgca ccgcgacgtc
     1021 aagctcgggt acgggggcat cagggagatc gagttcgtga gccaggcgct ccagctcctg
     1081 tacggaggca gggaccggtc actgcgggag aggagcaccc tcaaggccct ccacgccctg
     1141 tcccagaagg gcctgatcac ctacgaggag catgaggccc ttgcaaaggc ctacactttc
     1201 ctccggaccg ttgaacatcg catccagatc cttgacgacc tgcagaccca gaccatacct
     1261 acggatgaag aagacctgcg cgcgctggcg cgacgtacgg gttaccgtga acgcggaagc
     1321 gaggcggagc tcttgctccg tgaccatgcc gggcatacgc cgaaggtccg gcgcatctac
     1381 gacgagctct tcgcgttcac gggggacgag tctttaggca gcgggcccgc gggtgactac
     1441 ggccggctgc tcgatccgga aacgaccgag caggaggcgg tctcgctcct cgggaggctc
     1501 gggttcaggg accccggaaa ggcataccgg aacctgctcc tgctgcggga agggccggcc
     1561 ttcgtccacc agacgccgcg aagccgcaag ctctttgatg aaatattccc cgccctgttc
     1621 caggaaatcg tcgcctcgcc ggacccggac atggcgctca accatctcga atcgttcctc
     1681 gcggcgcagg gctcgtggga atcatttcag tccctcgtga agctcgatgc ctcggccttc
     1741 aaggtcctga tcgccgtttt cgcgaacagt gaatacctct cgaggatgct cgtgggcagg
     1801 ccggtcctgc ttcagaacct cctggagtcc cgcagggcgt tcggcccggg ggtatgcggg
     1861 aggtacgcga aagagctcgc aggcgccctg gagaagggag gagacatcgc ggaaaagctc
     1921 gatgccttga ggcggttcaa acaccaggag gagatacggc tcggaatggc ggaccttctc
     1981 aaccaggcgc ggcagacgac cgtttcgcgc gacctgtcga agctggcgga gatctgtctc
     2041 ggcgcggccc tcggcctggc ggcggaggag accggcaggc gctatggggt gtcgggagcc
     2101 aggggcccgg aatcgggggc cgccgggctt gcgatcatcg gcatgggcaa gcttggcggc
     2161 agggaactga cgtacggttc ggacctcgac atcc
//
LOCUS       DASSLD010000632         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_251135,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000632 DASSLD010000000
VERSION     DASSLD010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251135"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VFK23_11425"
     CDS             complement(<1..218)
                     /locus_tag="VFK23_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319738.1"
                     /translation="MQYIPLAAPFFLALFLIYVFVVVLIEFRILQYTFEKVGVDRRYM
                     FFLLLFSFLGSYINIPVAQLPPERLYTD"
     gene            406..537
                     /locus_tag="VFK23_11430"
     CDS             406..537
                     /locus_tag="VFK23_11430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319739.1"
                     /translation="MGTLTNSFADTVSVIDIMKRNVSTTVKVGKGPNGISVSSSGPQ"
     gene            783..1766
                     /locus_tag="VFK23_11435"
     CDS             783..1766
                     /locus_tag="VFK23_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7319740.1"
                     /translation="MKRLLALIVVAMFAVQPAYAETIKINLHSCCVTEDATTKAILPA
                     FRKYYKEKFGKDVDVSASFAGSGTLKNQILGGAPVQVAVLSSELYLDQLKEKGLISSD
                     WRKNPNRGVVIKSVIVFVTRKGNPKGLATFEDLARPGTKVLHASPDTSGGAQWAIFAI
                     YGAGANFRNLSFGPEDGGVKLLSAVEKNVIAQPESARQTFVQFDAGFGDAIVTYENEA
                     LLEKAKGRDYAISVPRKTIETEWKVARIDRNIRPEQEHAVSELIKCLYTEESQRSYAK
                     FGFRPVDKKIGKEFHAKYAQVAEPFTVDDIGGWKDARKNVIENTWKKTQVR"
     gene            1778..>2096
                     /locus_tag="VFK23_11440"
     CDS             1778..>2096
                     /locus_tag="VFK23_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319741.1"
                     /translation="MSRPLNMDSLVRGATFAAALGLFFLLILLPLVSLNQYAVREGIG
                     VFWDRLNNPDAVGALRLTALVALAATAVNAVIGTTMAFLLMRYRFPGRKVINALVDIP
                     LAIP"
BASE COUNT          483 a          601 c          613 g          399 t
ORIGIN      
        1 ctgtccgtat acaaccgctc cggcggaagc tgggcaacgg gaatgttgat atagcttcca
       61 agaaaggaaa atagcagcag aaaaaacatg taccggcggt ctacccccac cttttcgaag
      121 gtgtattgga gtattcgaaa ctcaatcaga acaacaacga aaacatagat aagaaaaagc
      181 gcaagaaaga acggagcggc gagtggaata tattgcattc gttcgtttcc tttctcttct
      241 tcgtcatttc ccgaatgatg atgatggcca ggaggaagac aagttgccct ggaaggagat
      301 cgaccccatt ttcacgcccc ggctttcgct caagcgttgg ggaccgcaaa tgcaatattt
      361 ttaaattatt gcatactgta tacagacaca caatgcaggt cggaaatggg gacgttaacc
      421 aattcttttg ctgatacggt atcggtcatc gacatcatga aacgaaacgt atcgacaaca
      481 gtgaaggttg gcaagggtcc gaacggaatc agcgtgtcct cttccgggcc gcagtaatcc
      541 cgactcgaaa gaatcaggaa agcggaggga ctcatcgcac ggtgagtccc tccccaccgt
      601 taacgtgcag ccgtaattac cttccaattt gggcatgtgg gggcatcgag cgcttccccc
      661 aatttttttc ttgacagaat cgatcaaaaa tatacgctac ttatcctata gggtaaggta
      721 acattcccct gttccgccgg gagcagctta ccctacaaaa tctacagctt ttcggaggta
      781 tcatgaagcg cctgctcgcc ctgatcgtcg tcgcgatgtt cgccgtccaa cctgcatacg
      841 ccgaaacgat caagatcaac ctccactcct gctgcgtcac ggaggacgcc acgacgaagg
      901 cgatcctccc ggcgttcagg aagtactaca aggagaagtt cggcaaggac gtggacgtct
      961 cggcctcctt cgccggctcg gggacgctga agaaccagat cctcggcggg gcgccggtgc
     1021 aggtggccgt cctctcctcc gaactctacc tggaccagtt gaaggaaaag gggttgatct
     1081 cctccgactg gcggaagaat ccgaacaggg gcgtggtgat caagtccgtc atcgtcttcg
     1141 tcacgcgaaa agggaacccg aagggcctcg ctacgttcga ggacctcgcc cggcccggca
     1201 cgaaggtcct ccacgccagc cccgacacgt cggggggggc gcagtgggcg atcttcgcca
     1261 tctatggcgc gggtgcgaac ttccggaatc tctctttcgg gccggaggac ggaggcgtga
     1321 agctcctctc cgcggtggag aagaacgtga tcgcccagcc cgagtcggcc cgccagacct
     1381 tcgtccagtt cgacgccggg ttcggcgacg ccatcgtcac ctacgagaac gaggcgttgc
     1441 tcgagaaggc caagggccgg gactacgcga tctccgtgcc aaggaagacg atcgagacgg
     1501 agtggaaagt ggcgcgtatc gacaggaaca tccgtccgga gcaggagcac gcggtgagcg
     1561 agctcatcaa gtgtctctac accgaggaat cccagcgctc ctacgcgaag ttcggttttc
     1621 gacccgtcga caagaagatc gggaaggagt tccacgcgaa gtacgcacag gtggcggaac
     1681 cgttcacggt ggacgacatc gggggatgga aagacgcacg gaagaacgtg atcgagaaca
     1741 cgtggaagaa aacacaggtg aggtaggccg caatacgatg agccggcccc tcaacatgga
     1801 ctccctggtg cgcggcgcga ccttcgccgc cgctctgggg ctcttctttc tcctgatcct
     1861 cctcccgctg gtgtcgctga accagtacgc cgtgcgggag gggatcgggg tgttctggga
     1921 ccggctgaat aacccggatg ccgtcggcgc cctgcggctg accgccctcg tggcgctggc
     1981 cgccaccgcc gtcaacgccg tcatcgggac gacgatggcg ttcctcctca tgcgctaccg
     2041 gtttccgggg aggaaggtga tcaacgcgct cgtggacatc ccgctcgcga tcccga
//
LOCUS       DASSLD010000633         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_819666,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000633 DASSLD010000000
VERSION     DASSLD010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819666"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFK23_11445"
     CDS             complement(<1..191)
                     /locus_tag="VFK23_11445"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HET7319742.1"
                     /translation="MNNEIVRKKTKQITLGPVKIGGGAPVAVQSMCNTDTRDAETTLS
                     QIRLLETAGCEIVRLAVPD"
     gene            complement(346..1629)
                     /gene="rseP"
                     /locus_tag="VFK23_11450"
     CDS             complement(346..1629)
                     /gene="rseP"
                     /locus_tag="VFK23_11450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET7319743.1"
                     /translation="MTLVYFLIVIGVLVFVHEFGHFVMAKRAGVRVEKFSLGMGPRIA
                     GFKKGDTEYIISALPLGGYVKMAGENPDEEPTGAPDEFQSKTVWQRAKIAATGPLTNI
                     VLAFLVMPLVFMVGTYAEGPAKVGFVEKDSPAERAGFLAGDVIEEVNGRHISDWTKAL
                     SLIAVNPGTDVTVTIDRGGEKKVLVLRPESATELKIGMSGLVPDMPAEIGKLKPGFPA
                     EKAGIKPNDKILAVNRQTIYHWNQFSTLVKDSGGRMLTLDVEREGKRLEVPVTPVQDG
                     GRYVIGVEPMMRLVFKKYGFFESLRLGFDKTIEAIDLTFITLKKLLTFSLSIKTLGGP
                     VMIAQMSGQAAAAGLSSFLSLLAMISISLGILNLLPIPVLDGGLILFLVIEAIRKKPV
                     SRKVMEVSQSIGAAVLITLIAVVSYNDVMRMFFSK"
     gene            complement(1793..>2089)
                     /locus_tag="VFK23_11455"
     CDS             complement(1793..>2089)
                     /locus_tag="VFK23_11455"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF024684.4"
                     /note="catalyzes the NADP-dependent rearrangement and
                     reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to
                     2-C-methyl-D-erythritol 4-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET7319744.1"
                     /translation="GALTFEEPDTGRFPCLAYAFDALRAGGTMPAVLSAANEVAVGCF
                     LEGKIGYGDIARVIRSTMDAHTPAPVGTVEDALAADLWARGEAERIIEHGKVGK"
BASE COUNT          411 a          648 c          587 g          443 t
ORIGIN      
        1 ttatcaggga ccgcgagccg aacgatctcg caccccgccg tttcaagcag cctgatctgg
       61 gagagcgtcg tctccgcgtc gcgggtgtcc gtgttgcaca tggactggac cgcgaccggc
      121 gcgccgccgc cgattttgac gggaccgagg gtgatctgct tggttttctt tcgtacaatt
      181 tcgttattca tgttaaggga agctagaatc gacgaccacg gagaaaatca atcaacacac
      241 ttttgtcatt cccgcgaaag cgggaatcca ttttgaagtt gatttgctca gagccaatgg
      301 atacctgctt ccgcaggtat gacaaataaa tccaccgggg tgtcgttatt tgctgaaaaa
      361 catcctcatg acgtcgttgt aggacacgac cgcgatcagc gtgatcagca ccgccgcccc
      421 gatgctctgg gacacttcca tcaccttcct gctcaccggt ttcttgcgga tggcctcgat
      481 cacgaggaac aggatcagcc cgccgtccag gacggggatg ggcagcaggt tcagaatccc
      541 gagcgagatg ctgatcatgg cgagaagcga gagaaacgag gacagaccgg cggcggcggc
      601 ctggcccgac atctgcgcga tcatcaccgg cccgccgagt gtcttgatcg acaggctgaa
      661 ggtcagaagt ttcttgagcg tgatgaacgt gaggtcgatg gcctcgatcg tcttatcgaa
      721 cccgaggcgc aacgactcga aaaagccgta cttcttgaag acgagccgca tcatcggctc
      781 cacgccgatc acgtaccgtc ctccgtcctg aaccggggtg acgggcacct ccaggcgttt
      841 tccttcccgc tcgacgtcga gggtgagcat cctgcccccg ctgtctttca ccagggtcga
      901 aaactggttc cagtgataga tcgtctgccg gttcacggca aggatcttgt cattcggctt
      961 gattcccgcc ttttccgcgg ggaaccccgg tttcaatttg ccgatctcgg cgggcatgtc
     1021 gggtaccagg ccggacatgc cgatcttcag ttccgtggcc gattccggcc tcaggacgag
     1081 tacctttttc tcgccgccgc ggtcgatcgt aaccgtcacg tccgtgccgg gattcacggc
     1141 gatgagggac agcgccttgg tccagtcgct gatgtgccgg ccgttgactt cctcgatcac
     1201 gtcgccggca aggaagcccg cccgctcggc gggagaatcc ttttccacga agccgacctt
     1261 tgcgggcccc tcggcgtagg tgccgaccat gaagacgagc ggcatgacaa gaaacgcgag
     1321 gacgatgttg gtgagcggcc ccgttgccgc gatcttggcg cgctgccata cggtcttgga
     1381 ttggaactcg tcaggagctc cggtcggctc ttcatcggga ttctcgcccg ccatcttcac
     1441 gtacccgccg agcggcagcg cggagatgat gtactccgtg tcgcccttct tgaagcctgc
     1501 gatcctgggc cccatcccga gcgagaactt ttccactcgc actcccgccc gctttgccat
     1561 gacgaagtgg ccgaactcgt gcacgaacac gagtaccccg atcacgatga gaaaatatac
     1621 taatgtcatt gcctctcctt acgacgtggt atcatcacat gttgtcggta tatcagtata
     1681 gatgtaatga cgctgagaag gtgagcgggt aggaaggtgg gaaaaaaccg aaatcacaaa
     1741 cagtactcat cttgccttct caccctccta ccttcccacc ttccagcttt aattactttc
     1801 ctaccttccc gtgttctatg attctctccg cttcccccct cgcccagagg tctgccgcga
     1861 gggcgtcctc gacggtcccg accggggccg gcgtatgggc gtccatcgtg gagcggatca
     1921 cgcgtgcaat gtcaccgtac ccgattttcc cctccagaaa acatccgacc gccacctcat
     1981 ttgccgccga gagaaccgcg ggcatggtgc cgcccgccct gagcgcatca aaggcatagg
     2041 ccaggcaggg aaaccggccc gtatcgggtt cctcgaaggt gagcgcgcc
//
LOCUS       DASSLD010000634         2594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_732954,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000634 DASSLD010000000
VERSION     DASSLD010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732954"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(809..2215)
                     /locus_tag="VFK23_11460"
     CDS             complement(809..2215)
                     /locus_tag="VFK23_11460"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7319745.1"
                     /translation="MLPSSNKKTLLVIDDEKVLCDTVRDYLENDRLEVLTAHTGAEGL
                     AVCTDRKVDVVLLDQRLPDGEGHALAPTILDRNEQAKIIFVTAFPSFDNAVAAVRLGA
                     HDYLSKPFDIEELKLTVDNALRTLDLERVEQIQNYAREKDRETAVIVGDSGPFSEVLE
                     HVAMAAEMDGPVLITGETGTGKNLVAKAIHYRSSFAGSAFVSINCAALPENLIEAELF
                     GHEKGAFTGASGAKKGLFEVADGGTVFLDEIGDMPMHLQAKLLSVLEDGTMRRIGSTA
                     VTPVNVRIIAATNADLESVLGKTFRKDLYYRLSVVRIHLPPLREHAEDIPRLCSHLLS
                     KTPAGRNIELPESELMNLQKYDWPGNVRELRNVLERALFLQKGPVLSPSLLLERYGTM
                     SMCQMKTASLVSNGLTTLEQAEQEAIRRAITALSGNLSKTSKALGISLSTLKRKLRAS
                     GGISSLIPGNDIRTKREQ"
BASE COUNT          583 a          682 c          691 g          638 t
ORIGIN      
        1 ggccgggtcc cggtgggggt actacaggct catttcctcc actggttggt ccgggacctt
       61 cactgctgct tttatgacag gcaactgcaa ccatcaatac tgccatgaaa attacgaata
      121 ccgccaacaa cttcttcata tgaaacctcc ttaaggactc gacattcgtc gaactcagtc
      181 attgtgtcat attctagcag atctttttta taaagacggc ctgaaaccac ggatggtttc
      241 ataacctcta tctatttgta tccacctccc aacgttaatc gactttatat acatgagata
      301 ttgtcgagaa gttatattaa tacataagtc ctatgtggca ttgggtgtga cattaaagga
      361 gttgaatttc ataaagcaaa gtatgtgcca gacgcgacac tatttaacat caccgacaaa
      421 acaaatagtt atgaaaaaaa tagacagaaa gatttaaata tcgtgtcatt atggaccgat
      481 caaattgaat gagtacgaca atatgaaccg actagctgaa gttgcatttt ttatttccgc
      541 taacgctatt actaattcta ataattgatc gcagaaaatc catgggtaga aatacctatt
      601 taccttcgac cctgtttatc gactgtcctg tatctctcgc actcttcgaa attgtttccg
      661 caataataga tcgcctcatc gatgttctgg ctgtttatgt tggcgataaa gcactcctcg
      721 aacgggtttg ggacgaaggg gcagtgccgc cccggcatcg atgcgatccg tccctgcatt
      781 tcctgattcg aaatacgaat ttttttcatt actgttcccg tttcgttcgg atatcgttgc
      841 caggtatcaa gctggatatc ccgcccgacg cccgcagttt tctcttgagc gtggaaaggg
      901 agatgcccag ggcctttgag gtcttcgaca gattgcccga gagagcggta atggcgcgcc
      961 ggatggcctc ctgttccgcc tgttcgaggg tcgtgagccc gtttgagaca agggacgcgg
     1021 tcttcatttg gcacatgctc atggttccgt acctttccag cagcagcgac ggcgacagga
     1081 ccgggccctt ttgaaggaac agcgcgcgct ccaaaacgtt ccgaagctcg cgtacgttac
     1141 cgggccagtc gtacttctgg agattcatca gctcggattc gggcaactcg atgttccgcc
     1201 ccgccggggt cttcgaaaga aggtgggaac agagccgggg gatgtcttcc gcgtgctctc
     1261 ggagcggcgg aaggtgtatg cggaccacgc tcaagcggta atacaggtcc ttgcggaacg
     1321 tcttgccgag gacgctttcc agatctgcgt tcgtagcggc tatgatgcgc acgttcacgg
     1381 gcgtgacggc agtgctgccg atgcggcgca tggtgccgtc ctccagaacg gagaggagct
     1441 tggcctggag gtgcatcggc atgtcgccga tctcgtccag aaaaaccgtc cccccgtccg
     1501 ccacttcgaa gaggcctttc ttggcgcccg atgcgccggt gaaggcccct ttctcgtggc
     1561 cgaaaagctc tgcctcgatc aggttttccg gcaacgcggc gcagttgatg ctgacgaagg
     1621 cggagcccgc gaaggagctc ctgtagtgga tcgcctttgc cacgaggttt ttgccggtgc
     1681 cggtctcgcc ggttatcaga acgggcccgt ccatttccgc ggccatggcc acgtgctcaa
     1741 ggacctccga gaaggggccg ctgtccccga cgattaccgc cgtctcacgg tccttttcgc
     1801 gggcataatt ctggatctgc tctacccgct caaggtccag cgtccggaga gcgttgtcaa
     1861 ccgtgagctt aagttcttca atatcgaacg gcttcgacag gtagtcatgc gcacccaggc
     1921 gcaccgccgc cacggcattg tcgaagctcg ggaaggcggt gacaaagatg atcttggcct
     1981 gttcgttccg gtcgagaatc gtgggcgcaa gggcgtgccc ttccccgtcg gggagcctct
     2041 ggtccagcag gacgacgtcg acctttctgt cggtgcagac cgcgagccct tcggctcccg
     2101 tatgcgcggt caggacctcg aggcggtcgt tttcgagata gtcccgcact gtgtcgcaca
     2161 acaccttttc gtcgtcgatg acgagcagtg tctttttatt tgagctgggc agcaccgtgt
     2221 ccctccggta acgtaaagtg aactttgttt gtcattcacg cgaaagcggg aatccatccc
     2281 cgattgaata cactaagacg acaatcaatt tacgtgccag agcggttcat tcggcggtac
     2341 tgtcgcttct ccaaagaaat taagcgattg agtgctgcat gaggaagcag gacggaaagg
     2401 aaaggtgtgg cgcgccgcaa ttgtgtggcg ccacagatgt ggagttacag tgaaatatat
     2461 ctacccgggt caaaaccatt tgccgcaaat ggccttaaaa atgaaccggc cacttagaga
     2521 aacacgcaag gaacgcagag ggattctaaa attgaaatac acgatttcgg ttttttcttt
     2581 agagttcttt gcgt
//
LOCUS       DASSLD010000635         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_145014,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000635 DASSLD010000000
VERSION     DASSLD010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145014"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFK23_11465"
     CDS             complement(<1..234)
                     /locus_tag="VFK23_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7319746.1"
                     /translation="MSIFFLLIVVVYGGMHVLAFLRARSAFAFGPAPGAALALFMLFM
                     VFAVFLIRALENLDYEISARALAYFAYCWMAALF"
     gene            complement(231..1310)
                     /locus_tag="VFK23_11470"
     CDS             complement(231..1310)
                     /locus_tag="VFK23_11470"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET7319747.1"
                     /translation="MRIGVLSGGGDAPGLNAVIRAVVKSAIFKYGWEVTGVIDGFEGL
                     LTPTKTRQLTSWNTRGILFVGGTILGTTNRGNPFAHKTVVGGKEIVEDLSPVVLNNIR
                     ELGLDALVVIGGDGSLKIGAGLLNMGVPVIGVPKTIDNDLMATYVTFGFQTAVDTATD
                     ALDKLHTTAESHQRVIVLEVMGRYAGWIALEAGMAGSADVILIPEIPFDMDRVAERIS
                     ERKKAGSKSSIVVVAEGAKPRGGEMAVKECASDGYALRLGGMGEIVGAEISRRTGFET
                     RVTVLGHLQRGGSPCPFDRLLATRYGAAAVELIAQKKFGEMVSYQPPAITSVPLETAI
                     CSLKLVEPEGELVKIAEGMGVSFGR"
     gene            complement(1407..>2024)
                     /locus_tag="VFK23_11475"
     CDS             complement(1407..>2024)
                     /locus_tag="VFK23_11475"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7319748.1"
                     /translation="GLPAGSREEIRSEILKLKAAGADIIKVMASGMVSLKSPGTITPG
                     GFPYDELAFIVEEARRHGLGVMAHANGEQAIMDASRAGVLSIEHGFFMTMRAVETMAE
                     KGTFWTPTVGALARAREAGGVSDKARAYIAGLIRSHLNMIRYAHDMGVPLAIGTDCVL
                     PDEDYREAYDAELRFFEQTGLSRETVMAIACEAGARLLGIGEERR"
BASE COUNT          383 a          674 c          582 g          385 t
ORIGIN      
        1 gaagagcgcc gccatccagc agtaggcgaa gtacgcgagc gctctcgcgg agatttcgta
       61 atcaaggttt tcaagcgcac ggatcagaaa aacggcgaag accatgaaga gcatgaacag
      121 cgcgagcgcg gcgcccgggg cgggaccgaa ggcaaaggcg ctcctggcgc gcaggaatgc
      181 gaggacgtgc atgccgccgt agacaacaac aatgaggaga aagaagatgc tcatctgccg
      241 aaactgaccc ccataccttc ggcgatcttg acgagttcgc cctccggctc cacgagcttg
      301 aggctgcaga tcgccgtttc gagcggtacg gaggtgattg ccgggggctg gtacgaaacc
      361 atctcgccga acttcttctg cgcgatcagt tcgaccgcgg ccgccccgta gcgcgtggcc
      421 aggagacggt cgaagggaca ggggctgcct ccgcgctgca gatggccaag caccgtgacg
      481 cgggtttcga agcccgtgcg cctgctgatc tcggctccca cgatctcgcc catgccgccc
      541 aacctgagcg cgtacccgtc ggaggcgcac tccttgaccg ccatctcccc tccccggggc
      601 tttgcgccct ccgccacgac cacgatgctc gacttgctcc ccgccttttt ccgttcgctg
      661 attctctcgg ccaccctgtc catatcgaag ggaatctcgg ggatgaggat gacgtcggcg
      721 ctccctgcca tgcccgcttc gagcgcgatc catcccgcat accgtcccat gacttcaagg
      781 acgatcacgc gctgatggct ctcggccgtc gtatggagct tatcgagcgc gtcggtcgcg
      841 gtgtcgaccg ccgtctggaa cccgaaggtc acgtaggtcg ccatgaggtc gttgtcgatg
      901 gtcttcggaa cgccgatgac gggaacgccc atgttgagca gccccgcccc gatcttgaga
      961 gaaccgtcgc cgccgatgac gacgagcgcg tcgagcccca gttccctgat gttgttcaga
     1021 acgacgggag acaggtcctc gacgatctcc ttgccgccca ccacggtctt gtgggcaaag
     1081 ggatttcccc ggttcgtggt cccgagaatg gtgccgccga cgaacaggat tccgcgcgtg
     1141 ttccacgaag tgagctggcg cgtcttggtg ggggtaagca gcccctcgaa cccgtcgatg
     1201 acgcccgtca cctcccagcc gtacttgaaa atagccgact tgaccacggc gcggatgacg
     1261 gcattaaggc ccggcgcgtc gccgccgccc gacagcactc cgattcgcat gagaacctcc
     1321 gtggaaatgc ctgaagttaa gaaggtggga acgtatgaag gtaagaaaaa aagcattttc
     1381 tcccaccttc ctaccctctt accatcctac cttcgttctt ccccaatccc caaaagcctc
     1441 gcccctgcct cgcaggcaat agccatcacc gtttccctgg agagacctgt ctgttcaaag
     1501 aaccggagct ccgcgtcata ggcctcccgg tagtcctcgt caggcagaac gcagtctgtt
     1561 ccgattgcga gcggtacacc catgtcatga gcataccgga tcatgttcag atgagacctt
     1621 atcagacccg cgatgtacgc tctcgctttg tccgataccc cgcccgcttc cctggcccgc
     1681 gcgagcgccc cgaccgtggg cgtccagaac gttccctttt ctgccatcgt ctcgactgcc
     1741 cgcatggtca tgaagaaacc gtgttcgatg gagaggacgc ctgcgcggga ggcgtccatg
     1801 atcgcctgct cgccgttcgc gtgggccatg acgccgagac cgtgtcttcg cgcttcctcc
     1861 acgatgaacg ccagttcatc gtacgggaag ccgcccggag ttatcgtccc ggggctttta
     1921 aggctgacca tccccgaagc catgaccttt attatatcgg cgcccgcagc cttgagcttc
     1981 aggatctccg atcgtatctc ctcccggctg cccgcgggaa ggcc
//
LOCUS       DASSLD010000636         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1188634, whole genome shotgun sequence.
ACCESSION   DASSLD010000636 DASSLD010000000
VERSION     DASSLD010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188634"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFK23_11480"
     CDS             <1..399
                     /locus_tag="VFK23_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HET7319749.1"
                     /translation="ITLISLLFTIIVMFSLKGNYIVKLPLDVLRIAVPLLIYFVVMFL
                     VSFYLGKKIGADYPKTATLSFTAASNNFELAIAVAVAVFGINSGAAFAAVIGPLVEVP
                     VLIGLVNVAKYFQRTYFSVEFCRIKSTDTL"
     gene            433..1149
                     /locus_tag="VFK23_11485"
     CDS             433..1149
                     /locus_tag="VFK23_11485"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HET7319750.1"
                     /translation="MRLAVFGDIHGNIEALKAAYDAAMQSKAEKIYHLGDLGGYSPFV
                     NEVVDFLIEHNIDGVQGNYDETVANDREHCGCKAEDTVQEEMANRSFLWTKEHASTKT
                     KDYLRDLPFSLSVISAGKKVLLFHATPSKKNLYWHEDRPEKFFKEMAEKVDADVMIYG
                     HTHKPYRKDFGGKIFINAGSVGKPKDGDPRACVALIDIGADNVSVEFLRVPYDVEKAA
                     QAIAASGLPVYFAAKLKEAK"
     gene            1146..1523
                     /locus_tag="VFK23_11490"
     CDS             1146..1523
                     /locus_tag="VFK23_11490"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET7319751.1"
                     /translation="MKFRILVLSALFALFCPAFSTAGGLDDTLNKAKAEGKIVMLELG
                     SVGCIPCEQMKPVMAKLSDSYKGKMEVIFIDVRKDHDTAHRFKVFMIPVQVFLGRDGK
                     EFHRHMGFYPYEEIVPMLKKAGI"
     gene            1590..2282
                     /locus_tag="VFK23_11495"
     CDS             1590..2282
                     /locus_tag="VFK23_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET7319752.1"
                     /translation="MMEFLLINIQSIIHTNQGLAFLAVFIGGLISATSPCVLAAIPRI
                     IGYVGGYSGGDKKKAAVYSLVFILGLSITFTVLGAAAAAMGQFLSFTGRWLYLGLAVI
                     AVAMGLQLMGLISIPLPFQITHRAKSRGLWGAFLLGLLTGTVSSPCATPVLAVILAYV
                     STQGDILYGSSLLFAYAVGHCALIFVAGLSIGLTESIVSSKGVKNFSLYSKRVSGALL
                     TVAGIYIGIANF"
BASE COUNT          551 a          634 c          725 g          578 t
ORIGIN      
        1 atcaccctca tttcgctctt attcaccatc attgttatgt tctccctcaa gggaaactat
       61 atcgtgaagc tgcccctcga tgtgctgcgc atcgcggtcc cgctcttgat ttatttcgtg
      121 gtcatgttcc tggtctcgtt ctatctcgga aagaagatag gcgctgatta cccaaagact
      181 gccacgctct ccttcaccgc tgcgagcaac aatttcgagc ttgccatcgc cgtcgctgtc
      241 gccgtcttcg ggatcaattc gggcgctgcc tttgccgcgg tcatcggccc gctggtcgag
      301 gtgcctgttc tgatcggcct ggtgaacgtg gcaaagtatt tccagcgaac ctatttcagc
      361 gttgaattct gcaggatcaa aagcactgac accctttgat gtaagcttga cgaacatttt
      421 tgaggtgatg atatgaggtt ggcggtattc ggtgacatcc atggaaacat cgaggcgctg
      481 aaagccgctt atgatgcggc aatgcagtca aaggcggaaa agatctatca cctcggcgac
      541 cttggtggtt actcgccgtt cgtaaacgag gtagttgatt ttctcatcga gcacaatatt
      601 gacggcgtgc agggaaatta cgacgaaacg gttgcaaatg accgggagca ttgcgggtgc
      661 aaggccgagg acacggtgca ggaggagatg gcgaaccggt cctttctgtg gacgaaagag
      721 cacgcgtcaa cgaagacaaa agattatcta agagacctgc cgttttctct atccgtcatc
      781 tctgcgggga agaaggtact gctcttccat gcgacgccct cgaagaaaaa cctgtactgg
      841 catgaggaca ggccggagaa gttcttcaag gaaatggctg aaaaagtcga tgccgatgtg
      901 atgatctacg gccatacgca caaaccgtac cgaaaggatt tcggcgggaa aatcttcatc
      961 aatgccggga gcgtgggtaa accgaaggac ggcgatccaa gggcatgcgt agcgctgatc
     1021 gatatcggcg cggataatgt gagcgtggag ttcctgcggg tcccttatga cgtggaaaaa
     1081 gcggcacagg cgatcgctgc gagcggctta cccgtgtatt ttgcggcgaa gctcaaggag
     1141 gcaaaatgaa gtttcggatt ctggtactgt cggcgctctt tgcgctgttt tgcccggcat
     1201 tcagcaccgc aggcggtctt gacgatacgc tgaacaaggc gaaggctgaa ggcaagatcg
     1261 tcatgctcga actcggatct gtcggttgca tcccctgcga gcagatgaag cctgtgatgg
     1321 cgaagctctc cgattcctat aaggggaaga tggaggtcat cttcatcgac gtaagaaagg
     1381 accatgacac ggcgcaccgg ttcaaggtgt tcatgatacc ggtccaggtg ttcctgggca
     1441 gggacggaaa agaattccac cggcacatgg gcttctatcc ctatgaggag attgtgccca
     1501 tgctgaagaa ggcggggata taactgcgga aacggcgacg gggagacgcg gggccgtggc
     1561 gaggaaaagc tcaggggcgt cggggatgca tgatggaatt cttactgatc aatattcaat
     1621 ctatcatcca tactaaccag gggctggcgt tcctggcggt gttcatcgga ggcctgattt
     1681 ccgccacgag cccctgcgtg ctcgccgcca taccccgtat catcgggtac gtgggcggtt
     1741 attccggggg cgacaagaag aaggccgcgg tctattccct tgtgttcatc ctcggccttt
     1801 ccatcacctt tacagttctc ggggctgccg cggccgccat gggtcaattc ctgtctttta
     1861 caggccggtg gttgtatctc ggccttgcgg tgatcgcggt ggccatgggg ctccagctca
     1921 tggggctcat ctcgatcccg cttcctttcc agataacgca tcgagccaag tcccgcggcc
     1981 tctggggggc attcctcctc ggccttctga ccggcacggt ctcgtctccc tgcgccactc
     2041 cggttctggc tgtgatcctg gcttatgtct cgacgcaggg cgatattctg tatggcagtt
     2101 cgcttctctt tgcgtatgcc gttggtcact gtgcactgat cttcgttgcg ggtctttcca
     2161 tcggccttac ggagagcatt gtaagttcga agggcgtcaa gaacttctcc ctgtactcga
     2221 agcgagtgag cggtgcgctg cttaccgtgg ccgggattta tatcgggata gctaacttct
     2281 agccgcgaaa tgctaccatc ggcggggcaa ccggtaagga aatgcactat catgcaatga
     2341 ggtaccgcat ttggaacaac ggttgaaaga gagagattcg aggcaagccg gccatgaaca
     2401 gcggacaggc aatcaactat ccggcagagc gtgtgttaaa aaaaaagagg agctcaatac
     2461 atcaaggatg aaaaatcagg ctcagagg
//
LOCUS       DASSLD010000637         3225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1617318, whole genome shotgun sequence.
ACCESSION   DASSLD010000637 DASSLD010000000
VERSION     DASSLD010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617318"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1082
                     /locus_tag="VFK23_11500"
     CDS             63..1082
                     /locus_tag="VFK23_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET7319753.1"
                     /translation="MADKLNPRERVLKFLHGEKVDRPPIFSGMGNVIKPVLDKYGINF
                     AKVHRDPELMAKAAGGMYREFGFECAVVPFDLGVEAEALGARMNFYDEVEGLLYPTVK
                     EKSVTSAEDLKIPSDIRNAGRIPVVVKAIKALRRELGNDVAIGTYVLGPFTLAGQVKE
                     LDQLFEESFLEPEKTGRLLEKLSGFIVDVFKVYQEAGADYFTLREMGGTSDVMSPRSF
                     KSLIKPHLTSILAEMPRPNILHICGDTNAIITDMAECGADAVSVDQKNCIAETRQKLG
                     AGALILGNFDPIKVLHKGEAKDVGPAVLSSLKGGANAVWPGCDLWPEVPRENMEALMK
                     TLTKA"
     gene            1238..1855
                     /locus_tag="VFK23_11505"
     CDS             1238..1855
                     /locus_tag="VFK23_11505"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET7319754.1"
                     /translation="MKVVQIAGYLGSGKTTLIIALSKQIARDGTRVAILVNDVGVVPV
                     DGAVMQEYGLTVKDIGGGCICCQVAGSLMKTLDTLANDIRPDIVIIEPTGIAVPGAIR
                     QTVLLSARKHPVENGHTVVLFDTSRASKLLTYDTLKRLVSLQIKDATIIGLSKSDLVA
                     ASEIEAASRAVREINPHAEIVRLSAVSGEGLSLIAGRIKSAVGAA"
     gene            1852..2277
                     /locus_tag="VFK23_11510"
     CDS             1852..2277
                     /locus_tag="VFK23_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319755.1"
                     /translation="MKLPFKLQPENPAHEDISSYAVDRVFISPEGLGPGELRSLARDA
                     VRTVAAGCEAAGAKDVSHVKLYLEHESGFIHANAVSGNPEITVAGRDGVTAKQFRLVM
                     NAVIYGLSEYSIRTSVEQGIEAVQIQYGLTLVTAQGKNH"
     gene            2307..3125
                     /locus_tag="VFK23_11515"
     CDS             2307..3125
                     /locus_tag="VFK23_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HET7319756.1"
                     /translation="MTAHATEKSIQDYKIAQEPFYLPIQDEVELFKIAYENRLPLMLK
                     GPTGCGKTRFLSYMAYKINQPLITIACHEDLTASDLVGRYLLDGGTTRWQDGPLTLAV
                     RYGGICYLDEVVEARKDTTVVIHPLTDDRRVLPLEKRGQILEATDNFMLALSYNPGYQ
                     TVLKDLKQSTKQRFITLDFDYPSASLEQEIVVHESGVDHKTASELRKIAEKFRGLKDR
                     GLDEGVSTRLLIYAGVLITRGVEPRRACEVALIKPITDDPEIQKTLKEIVSAVL"
BASE COUNT          748 a          914 c          993 g          570 t
ORIGIN      
        1 gatcttctca caaatcaatc ccactaaaca aataacggac agacagaaag aaaggagttt
       61 acatggctga caaacttaat ccgagggagc gggtcctgaa gttccttcac ggggaaaagg
      121 tggaccgtcc ccccatcttc agcggcatgg ggaacgtcat caaacccgta ttggataaat
      181 acggaataaa ctttgccaag gttcaccggg atccggaact catggccaaa gcggcaggcg
      241 gcatgtaccg cgagttcggc tttgagtgcg cggtggtccc cttcgatctc ggcgtggaag
      301 ctgaagccct gggagcgcgg atgaattttt atgatgaagt ggaagggctc ctctatccga
      361 cggtaaagga gaaaagcgtc accagcgccg aggatctcaa gatcccttcc gacatcagga
      421 atgccgggcg gatccccgtc gtggtcaagg ccatcaaggc cttgagaagg gaactcggca
      481 acgacgtggc catcgggacc tatgtcctcg gccccttcac cctcgcggga caggtgaagg
      541 aactcgacca gttgttcgaa gagtccttcc tcgagccgga aaagaccggc aggctcctgg
      601 agaagctctc cggtttcatc gtagatgtct tcaaggtcta ccaggaggcc ggggccgact
      661 attttacgct ccgggagatg ggagggacct cggacgtgat gagtccgcgg agcttcaaga
      721 gcctgatcaa gccccatctc acgagtatcc tggccgagat gccgagaccg aacatcctgc
      781 acatctgcgg cgatacgaac gcgatcataa ccgacatggc ggaatgcggg gcggacgcgg
      841 tgagcgtgga ccagaagaac tgcatcgctg aaacgcgcca gaagctcggc gcgggggcac
      901 tcatcctcgg aaacttcgat ccgatcaagg tgctccataa aggggaagcc aaggacgtgg
      961 ggccggcagt gctgagttcg ctcaagggcg gggcgaacgc ggtctggccc ggatgcgacc
     1021 tctggcctga agtgccgcgg gagaacatgg aagccctgat gaagaccctg accaaggcgt
     1081 aacggcaaag atcaaatgcc agtctttaac cgcataggac acggaggtcc cgaaaaagac
     1141 aagagacaga tgtctcggtt ttagcttccg tctttactct gtggtctttg tggtaaatgc
     1201 tttcgtcgtc ggtttgcagt tctcggagtt ttcagccatg aaggtcgtac agatcgcagg
     1261 ctaccttggt tcggggaaga ccacgctgat aatcgcgctg agcaagcaga ttgctcgtga
     1321 cgggaccaga gtcgcgattc tcgtgaatga cgtaggcgtg gtgcccgtag acggggcggt
     1381 catgcaggaa tacggcctca cggtcaagga catcgggggc ggctgcatct gctgccaggt
     1441 ggcgggaagc cttatgaaaa cgctggacac ccttgccaac gacatccgcc ccgacatcgt
     1501 catcatcgag cccacgggca ttgccgttcc cggtgcgatc cgccaaaccg tactactctc
     1561 cgcacggaag cacccggtgg agaacgggca taccgtcgtc ctgttcgaca cgtcgcgggc
     1621 ctcgaagctc ctgacttacg atacgctgaa gcgactcgta tcgcttcaga taaaggacgc
     1681 cacgatcatc gggttgagca aaagcgatct ggtcgcggcc tcggaaatcg aagcagcttc
     1741 acgggcggta cgtgagatca atccccatgc ggagatagtc cggttatcgg ccgtctccgg
     1801 tgaaggactt tctctgatag caggcaggat caagagtgct gtaggggcgg catgaaactc
     1861 cctttcaagc tgcagccgga aaatccggcc cacgaggaca tcagcagtta tgctgtagac
     1921 cgggtgttca tttccccgga gggcctcggc cccggtgaac tgcgttcact ggcccgcgac
     1981 gcggtccgga cggtggccgc ggggtgtgag gccgcaggcg cgaaagacgt gagccacgta
     2041 aaactgtacc tggagcacga atcgggattt attcacgcca atgcggtcag cggcaatccg
     2101 gagatcaccg ttgccggccg ggacggcgtc acggcgaagc agttccgcct ggtaatgaac
     2161 gccgtcattt acggcctttc tgaatactcc atccggacgt ccgtggaaca gggcattgaa
     2221 gcggtccaga tccaatacgg gctgacgctg gtgacagcgc agggaaaaaa tcattgaccg
     2281 gggagggaca ggaagtggag gagacaatga cggcgcatgc aacggaaaag agcatacagg
     2341 actacaagat tgcccaggag cccttctacc tgccgatcca ggacgaggtg gagcttttca
     2401 agatcgctta cgagaaccgg ctgccgctca tgctgaaagg ccccacgggc tgcggcaaga
     2461 cgcggtttct gtcctacatg gcgtacaaga tcaatcagcc gctcatcacg atcgcctgcc
     2521 acgaggacct taccgcgagc gacctagtgg gcagatatct cctcgatgga ggcacgacac
     2581 gctggcagga cggccccctc acccttgccg tccgctacgg cggcatctgc tacctggacg
     2641 aggtcgtgga ggcacggaag gacaccaccg tcgtgatcca tcccctgacc gacgaccgcc
     2701 gggtgctgcc cctggaaaag cggggccaga tcctcgaggc gacggataac ttcatgctgg
     2761 cgctctcgta caaccccggc tatcagaccg ttctcaagga cctgaagcag agcaccaagc
     2821 agcgtttcat caccctggac ttcgattatc cgtcggccag cctggagcag gagatcgtgg
     2881 tgcacgagag cggtgtcgat cacaagacgg catcggagct caggaagatc gcagaaaagt
     2941 tccgggggct caaggaccgg gggctcgacg agggcgtgag cacgcgcctc ctgatctacg
     3001 ccggggtact catcacccgg ggggtggagc ccagacgggc ctgcgaagtc gcgctcataa
     3061 aacccatcac cgacgacccg gagatccaga aaacactgaa ggaaatcgtg tcggcagtct
     3121 tatgaccact cgggacacca tacaggagca gattgccgca atcgcggcct cgatagcgga
     3181 gcaactcgga aaagatcagg tcgacggagc cgtgtcccgg ctgcg
//
LOCUS       DASSLD010000638         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_230266,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000638 DASSLD010000000
VERSION     DASSLD010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230266"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VFK23_11520"
     CDS             <1..909
                     /locus_tag="VFK23_11520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HET7319757.1"
                     /translation="AGVLEGHPLIDRLLVSRRKAWFRSLMNPLAFARGIGDMVRFVRE
                     LRRTRYDIAIDFQGLLKSGIVIGLARADRKIGFDRTRELSYLFLNERLPAYDVERHAL
                     ERYLDVARYLGAKDPSTSCLLPLEKERPVVSKRVGEIRAPGRPLVAVNPVARWKTKLW
                     QERKFAELADRLIREKNAVVIFTGSPGDRQVNERILSMMREKAVNWAGETSLKELAAL
                     ASLCDLFITTDTGPMHLAAAAGARVLALFGPTAPWRTGPYGPSNITVRTGIDCSPCFR
                     RKCGGHIECMSGIAVEDVMRKIPNFQ"
     gene            complement(1025..1462)
                     /locus_tag="VFK23_11525"
     CDS             complement(1025..1462)
                     /locus_tag="VFK23_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319758.1"
                     /translation="MVELVRVSDIEKKEKLKSYLEEHFKKGKYAEMTQAIGMYSILID
                     PEHTMSDQSGLYHFVLDLIKDRIRTTKGKGYAAWYTGFPKILDQMVSANQIVTDQAAA
                     DVLASQRAGSGPFKSVDDFFFWALDDRRLPLEQIVKYIETTAK"
     gene            1663..2862
                     /locus_tag="VFK23_11530"
     CDS             1663..2862
                     /locus_tag="VFK23_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7319759.1"
                     /translation="MKSSGWRRGLTRNVVILGFVSLLNDGASEMIYPLLPAFLTAVLG
                     AGPAALGIIEGIAESTASLLKLYSGYLSDRVKKRKGWIVAGYSLSNIIRPLIAFSTSW
                     LHVLALRFSDRVGKGLRTSPRDAIIADSTPPEFRGKAYGFHRAMDHTGAVVGPLAATA
                     LLLAFHDDVKTIFILSFIPGLLAVLMLLFGLREKISEGSRAATASFNFRSAWGEMTPG
                     FRKYLFIILVFTLGNSTDAFLLLRAQQLGVSVTLLPTIWVVLHVVKMSFSVPGGILSD
                     RIGRKKVIVAGWLVYALVYAGFGLASEHWHAWALFAVYGVYFGLAEGVEKALVADFAP
                     LHLRGSSFGLYHLIVGLGAFPASLLFGLIWQQFGSAAAFGAGAALALVASVMLSGLAV
                     AKPAEVK"
BASE COUNT          529 a          859 c          873 g          653 t
ORIGIN      
        1 gcgggcgtcc tcgaaggcca tccgctcatc gaccgcctgc tggtctcccg caggaaagcg
       61 tggttccggt cgctgatgaa tccgctcgcc ttcgcgcggg gcatcgggga catggtcagg
      121 ttcgtccgcg agctccgccg aacgcggtac gacatcgcca tagacttcca gggtcttctg
      181 aaaagcggga tcgtgatcgg ccttgcgcgg gccgaccgca agataggctt cgaccgcacg
      241 cgggagcttt cctatctttt cctgaacgag cgcctgccgg cctacgatgt cgagcggcat
      301 gccctcgaac ggtatctgga cgtcgcgcgg tatctcggcg cgaaggaccc gtcaacgtcg
      361 tgcctgctgc cgcttgagaa ggagcgtccg gttgtttcga agcgggtcgg agagatcagg
      421 gcgccgggac ggccgctcgt ggccgtgaac ccggtcgcgc gctggaagac gaaactctgg
      481 caggaacgga agtttgcgga gttggcggac cgcctgattc gggaaaagaa cgccgtcgtg
      541 atcttcacgg gaagccccgg cgaccggcag gtcaacgagc gcatcctgtc gatgatgcgc
      601 gagaaggccg tgaactgggc aggggagacc tcgctcaagg aactggccgc ccttgcgtcg
      661 ctctgcgacc tcttcattac gaccgacacg ggccccatgc accttgccgc cgctgcgggc
      721 gcccgggtcc ttgcgctctt cggccctacg gccccgtggc ggaccgggcc ctacggtccg
      781 tcaaacatca cggtccgcac ggggatagac tgttcaccct gcttccggag gaaatgcggc
      841 gggcacatcg agtgcatgtc cggcatagcc gttgaggacg tgatgagaaa aatcccaaat
      901 tttcaataac caatcccaaa ttacaaacaa tatccaatca tcaaattgcg aaccgaacgt
      961 ttggacattg gttattggtg cttatttgtt atttggcgct tgagatttcg tgcttaactt
     1021 tcattcattt tgccgtagtt tcgatgtact tcacaatctg ctcgagcgga aggcgacggt
     1081 cgtcaagggc ccagaaaaag aaatcgtcaa cggacttgaa aggtccggac cctgcccgct
     1141 gggatgcaag gacgtcggcc gctgcctggt cggtcacgat ctggtttgcg gaaaccatct
     1201 gatcgaggat cttggggaat cccgtatacc acgctgcata gcccttgcct ttggtggtcc
     1261 tgatccggtc cttgatcaag tcgagaacga agtgatagag acccgactgg tcgctcatcg
     1321 tatgttcggg atcgatcagg atggaataca tgccgatagc ctgggtcatt tccgcgtatt
     1381 ttcccttttt gaaatgctct tccagatacg acttgagttt ttcctttttt tcaatgtctg
     1441 aaacccgaac gagttccacc atgacaaatt acctccttgg tctcgactgc cggacgtatc
     1501 acaacggagg gattttatag caactgcggc gccgaggcaa gaagaatttc accctgaaac
     1561 gggtcaggcc tataacggca gaggcagcgc ttttcttcat tgttttcttg aaaaagtacg
     1621 ccgattcagg taaagtccca gctacctatg aaggaaacag cgatgaaaag cagcggatgg
     1681 cgcaggggcc tcaccaggaa tgtcgtgata ctcggcttcg tcagcctgct gaacgatggc
     1741 gcatcggaga tgatctaccc gctccttccg gcgttcctca cggccgtgct cggcgcgggg
     1801 ccggccgccc tcggaatcat tgaaggcatc gccgagtcta ctgcgtcact tctgaaactc
     1861 tattcgggct acctgtcgga ccgggtgaaa aaacgcaagg gctggattgt cgcaggctac
     1921 tcgctctcga atatcattcg tccgcttatc gccttttcca cgtcgtggct gcatgtgctc
     1981 gcgctccggt tctcggaccg cgtcggcaag gggcttcgca cctcaccccg cgatgcgatc
     2041 atcgcggatt cgacgccgcc ggagttccgg ggaaaggcgt acgggttcca ccgggccatg
     2101 gaccacaccg gcgccgtcgt cgggccgctg gccgcgaccg cacttctcct tgcatttcac
     2161 gacgatgtca agaccatctt cattctctcc ttcatccccg gcctgctcgc cgtgctgatg
     2221 ctcctcttcg ggctccggga gaagatatcg gaagggtccc gtgccgcgac tgcatcgttc
     2281 aatttccggt ccgcctgggg ggagatgacc ccgggcttcc ggaaatatct tttcatcatc
     2341 ctcgttttca cgttggggaa ctcaacggat gcattcctgc ttcttcgcgc ccagcagctc
     2401 ggcgtttccg tcacgctcct gccgacgata tgggtcgtgc tccacgtcgt caagatgagc
     2461 ttctcggtcc ccggagggat cctctccgac cgcatcggaa gaaaaaaagt gatcgtggcg
     2521 ggatggctgg tctatgccct ggtctacgcg gggttcggcc ttgcctccga gcactggcat
     2581 gcctgggcgc tcttcgcggt gtacggcgtc tatttcgggc tggccgaggg ggttgagaag
     2641 gcgctcgttg ccgatttcgc gcccctgcat ctgcgcggat cgtccttcgg gctgtatcat
     2701 ttgatcgtcg gtctcggggc ctttcccgcg tcgctcctgt tcgggctgat atggcagcag
     2761 ttcggatcag cggcggcgtt cggcgcgggc gccgcgctgg cgcttgtggc aagcgtgatg
     2821 ctttcgggtc ttgcggtcgc gaagccggcg gaggtaaaat agcctggctt tcattccggc
     2881 gtggctctgg ccggaatccg gtgaagatgc attt
//
LOCUS       DASSLD010000639         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1057667, whole genome shotgun sequence.
ACCESSION   DASSLD010000639 DASSLD010000000
VERSION     DASSLD010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057667"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..546
                     /locus_tag="VFK23_11535"
     CDS             280..546
                     /locus_tag="VFK23_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319760.1"
                     /translation="MVNANVEQLLDDSFIVFEGFIYLVDNRPQRSPITGTVEALKREL
                     SAREVRRCDIAGRRDIQIALACAEAARTDKNRKMAMSILGFQIR"
     gene            819..1700
                     /gene="xerD"
                     /locus_tag="VFK23_11540"
     CDS             819..1700
                     /gene="xerD"
                     /locus_tag="VFK23_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET7319761.1"
                     /translation="MNNELLNRFLNYLTVEKGLAHNTIQSYERDLRRYLEEIKAGGPD
                     DIRESDIVSFLTGLSRAGIAAPSVARCLSAIRGFHKYLLIDGLAATDPTVNLETPRGW
                     KRLPKTLSRGDVEALLDGPDPDNPVGLRDKAMLELLYATGLRVSELVGLRIGDLNLER
                     GFLIVIGKGAKERAVPMGEAAMAAVQVYLDRARRALLSGAESDHLFISMKRRGITRQM
                     FWERIKFYARKAGIAVPVSPHTLRHSFATHLLDNGADLRAVQAMLGHSDISTTQIYTQ
                     VSRERLRQIHEKYHPRR"
     gene            1808..>2214
                     /locus_tag="VFK23_11545"
     CDS             1808..>2214
                     /locus_tag="VFK23_11545"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET7319762.1"
                     /translation="MEVITSHTNADFDALASMVAAKKLYPEAKLVFPGSQEKSMRDFF
                     LESMFYALEVERLKNIDVDVITRLIIVDNRNPGRLGKLSAALKRPGVSVHIYDHHPPA
                     DGDIRGEKEIIEEIGATTTIMVALLRERGITITP"
BASE COUNT          487 a          643 c          655 g          429 t
ORIGIN      
        1 tcacgtttgg gcgccgacgc ggaccagccg caccggatca agtaccggca tttgacgaga
       61 cagtgacgcc gtggggtgtc aggcgagagc gtaacggtca aaagttctct gaacagcaaa
      121 gccatttaac cacagaggac gcagagggaa agcgaggcgg ggagacaagg attcgtttcc
      181 cttagattta aaccagagtc aaagatttcc tccgcgttcc tctgcgtcct ctgtggtgga
      241 atatttcaac aactcgaatt agttgccagg gagggcagta tggtcaatgc aaacgtagaa
      301 caattgttgg acgattcgtt catcgtattc gagggattca tctatctcgt ggataaccgg
      361 ccgcagcggt ccccgattac cggtaccgtt gaggcgctga aacgtgagct gtccgcgcgc
      421 gaagtgaggc gctgcgacat cgcaggacga cgagatatcc agattgcctt ggcctgcgct
      481 gaagccgcga ggaccgataa aaaccggaaa atggctatgt ccattctggg atttcagata
      541 cggtgactgc acgggagccc tctggtcgcc aaattgaaga cgcccgccat gaaacgccgc
      601 cgggagcttc ttcctttccc cgctctaccg ataaacccct gatgtcgtga tcgagaagcc
      661 gtggaaccgc accaggtttc tcaggtgcgg ttcgcgggaa acgatgaagt atgcgttggc
      721 ctcttaccga ctcacgatcg tccctcctca cgttcttgct ttttcagcgc ttctgacata
      781 gaatcaaatc agggccgatc cgtcccgttt gaattcctat gaacaacgag ctgctgaaca
      841 gatttctcaa ttatctgacc gtggaaaagg gtttggcgca caataccatc cagtcctatg
      901 aacgggacct gcgcagatac ctggaggaga ttaaagcggg tgggccggac gacatcaggg
      961 agtcggacat cgtttccttt ctcaccggcc tgtccagagc gggcatagcc gcgccgtccg
     1021 tcgcgaggtg cctgtcggcg atcagggggt tccataaata tctcctcatc gacggccttg
     1081 cggcgacgga cccgacggta aatcttgaaa cgccgcgggg gtggaaacgg ctgccgaaga
     1141 cgctttcccg cggcgacgtc gaggcgctgc ttgacggacc cgaccccgac aacccggtcg
     1201 gtctccggga caaggccatg ctcgagcttc tgtacgccac ggggctccgc gtgagcgaac
     1261 tggtgggcct caggatcggc gatctgaacc tcgaacgggg atttctcatc gtcatcggca
     1321 agggggccaa ggaacgcgcg gtacccatgg gggaggcggc catggccgcc gtgcaggtat
     1381 acctggaccg ggcgcgccgc gcgctgctct cgggcgcaga gtcggaccac cttttcatca
     1441 gcatgaagcg ccggggcatc acccggcaga tgttctggga gcggataaaa ttctacgcac
     1501 gaaaggcggg catcgccgtc ccggtctcgc cgcacaccct gcgtcattcc tttgcaacgc
     1561 acctcctgga caatggcgcc gacctccgag ctgtccaggc catgctcggc cactccgaca
     1621 tctccacgac gcagatctac acccaggtgt cccgggagcg gctccggcag atccacgaga
     1681 agtatcatcc gaggcggtga aacgaatgcg gaattcggaa tgaaaggcga gtgaaggagc
     1741 acaagcggtt gccccggtct tcgggttttt attccgaatt ccgaattccg cattccgaat
     1801 ttgaactatg gaagtcataa ccagccatac aaatgccgat ttcgacgccc tggcctcgat
     1861 ggtcgcggcg aaaaagctct atccggaagc gaagctggtg tttcccggct cccaggaaaa
     1921 gagcatgcgg gacttttttc tcgagtccat gttctacgcg ctggaagtcg agcgcctgaa
     1981 aaacatcgac gtggacgtca tcacccggct catcatcgtg gacaaccgga accccggccg
     2041 gctcgggaag ctctccgcgg cgctcaaacg gcccggcgtg tcggtgcaca tctacgatca
     2101 tcaccctccg gcggatggcg acatccgggg ggaaaaggaa atcatcgagg agatcggagc
     2161 cacgaccacg atcatggtcg cgcttttgag agagcggggg atcacgatca cccc
//
LOCUS       DASSLD010000640         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1155988, whole genome shotgun sequence.
ACCESSION   DASSLD010000640 DASSLD010000000
VERSION     DASSLD010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155988"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VFK23_11550"
     CDS             <1..694
                     /locus_tag="VFK23_11550"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HET7319763.1"
                     /translation="DLVANAYGIALMKKGDFASLNRARQTEGNKALLSAGTGLGAAIL
                     FWDGRRHIVSPSEGGHVEFGPKNRLELGLLLYLFERFGHVSYERILSGAGLFNIYQFL
                     RDTGVCGSEPRWLSLRIDEGDPAAVITETARLGKNRLCQAALHLFVSIYGAAAGNLAL
                     QVMANGGMYLGGGIAPKIIWKLRDGTFMKAFSNKGRLSQIVGRIPVKVIMNDRAALWG
                     AAAHASEMLKKR"
     gene            762..1394
                     /locus_tag="VFK23_11555"
     CDS             762..1394
                     /locus_tag="VFK23_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET7319764.1"
                     /translation="MSSQKITTLFLDIGGVLLTNGWDRSARRLAALKFSLDLSEMDER
                     HHLTFDTYEAGKLSLDAYLARVVFYEERSFSRDEFRRFMFDRSEPYPDMIDLVRGVKA
                     RHGLKIAAVNNEGRELSLHRIKTFNLNSFIDCFISSSFVHYRKPDADIYRTALDIVQA
                     SPPQVLYLDDRAMFVDVARGLGINGIHHKSYETTKAAMEAFGLYSTRAEQ"
     gene            1453..>2694
                     /locus_tag="VFK23_11560"
     CDS             1453..>2694
                     /locus_tag="VFK23_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017742184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET7319765.1"
                     /translation="MAMIRGLREAFGKPGIEPRWTQGNKNGVGTAYSATSRIWFTLWN
                     GAVTEVYYPTIDLPQIRDLQYLVSDGETFFHEEKRHLRTETRRISHHALSYEITNHGP
                     LDRYRIVKEVIAAPHLPCILQRTRIEGDDAFVSRLRLYALCAPHLKVGGRGNNGFVLE
                     VAGREILAAEKGGVWMALAASIPFSRLSCGYVGSSDGWTDLADNFRFDWEFDRAPDGN
                     IALTGEIIPQNGREFTLALAFGDGQHNAVTTLFQVLGFPFEEHHHRYVEQWNRACRKI
                     YPVREIDGDGGQLYHSSVSLLLAHEDKTYPGAFIASLSIPWGESKGDEDMGGYHLVWT
                     RDMVNTAMALLAAGNQETPLRALIYLAATQHDDGGFSQNFWINGESYWRGIQLDEVAF
                     PILLAWRLYRENALAEFDPYHM"
BASE COUNT          592 a          779 c          774 g          549 t
ORIGIN      
        1 cgatcttgtc gcaaacgcct acggcatcgc gctcatgaaa aaaggcgact tcgcatctct
       61 gaaccgggca aggcaaaccg agggcaacaa ggcgctcctc tccgccggga ccggactggg
      121 agccgcgata cttttttggg acggcaggag acacattgtt tccccgtccg aaggcgggca
      181 tgtcgaattc ggaccgaaga accgactgga actcggcctt ctcctgtacc tgtttgaacg
      241 cttcggccat gtcagctatg aacggatcct gtcaggtgcg gggcttttca atatctatca
      301 attcttaagg gacacgggcg tttgcgggag cgagccgcgc tggctttccc tgagaatcga
      361 cgagggagac ccggcagccg tgataaccga aacggcccgc ctcgggaaaa acaggctttg
      421 tcaggcagcc ctccaccttt ttgtctcgat ttacggcgcc gccgccggaa atctggcact
      481 ccaggtgatg gcgaacggcg gcatgtatct cggaggaggc attgctccga agatcatctg
      541 gaaactcagg gatgggacgt ttatgaaggc cttcagtaac aaagggaggt tgtcgcagat
      601 tgtcggacgc attccggtga aagttatcat gaacgaccgg gccgctttgt ggggggctgc
      661 cgcacatgcc tcggaaatgc tgaaaaaacg atgaaccgcg atgtgcagat gtcgcgggaa
      721 aaaacaatgc gccttcggat gagggatgca aaggcaattc aatgtcttca caaaaaatca
      781 caacgctttt tctcgacatc ggcggcgtgc tgctgacgaa cggctgggac cgctcagccc
      841 gcaggctggc cgccttgaaa ttcagtctgg acctatccga gatggatgag cgtcaccact
      901 tgacgttcga tacctatgag gccggcaaac tgtccctcga tgcatacctg gcacgggtcg
      961 ttttctacga agaacggtcc ttctccagag atgaattcag gaggttcatg tttgaccggt
     1021 ccgaacctta tccagatatg atagacctgg tccgcggcgt caaagcgcgg catggcctga
     1081 aaatagcggc agtcaacaat gaaggacggg aactttcgct ccatcgtatc aaaacgttca
     1141 accttaactc cttcatcgac tgtttcatct cttcgtcctt tgtccattac cgaaagcccg
     1201 acgcagatat ttatcgcacg gccctcgata ttgtccaggc atcaccgccg caggtcctgt
     1261 atctcgacga ccgggcgatg ttcgtcgatg tggcccgggg actggggata aacgggattc
     1321 atcataaatc ctacgagacc acgaaggccg cgatggaggc attcggactg tattcgacga
     1381 gagcggagca atgaaaatgg cgagctcaag aagggttttt catttctatc catctcattg
     1441 agggagacgc gaatggcaat gatacgcgga ttgcgcgagg ccttcggaaa accgggtatc
     1501 gagccccgct ggacgcaggg gaacaaaaac ggcgtgggta cggcctactc cgcgacgagc
     1561 cgcatatggt tcaccctgtg gaacggggcg gttaccgagg tatactatcc gaccatcgac
     1621 cttcctcaaa tccgcgacct ccagtacctc gtttcggacg gagaaacatt ttttcatgag
     1681 gaaaagcggc atctcaggac cgagacccgc cgcatctcgc accacgcgct ctcctacgag
     1741 atcacgaacc atggtccgct ggaccggtac cgtatcgtca aggaagttat cgccgcgccg
     1801 cacctcccct gtattctcca gcgcacgcgg atcgagggag acgatgcctt cgtttcccgg
     1861 ctccgcctct atgccctctg cgcgccacac ctcaaggtcg gcgggagggg caacaacggc
     1921 ttcgtgctcg aggtcgccgg acgggaaatc ctggcggccg aaaagggcgg cgtctggatg
     1981 gcgctcgccg cctccattcc cttttcacgt ctgtcctgcg gctacgtcgg ctcatccgac
     2041 ggatggaccg atctggccga caacttccgc tttgactggg agttcgatcg ggcgccggac
     2101 ggcaacatag cgcttaccgg agaaatcatc ccgcagaacg gacgggagtt cacgctcgcc
     2161 ctggccttcg gcgacggaca gcataacgcc gtcacgacgc tgttccaggt tctcggcttc
     2221 cctttcgaag agcaccacca tcgttatgtg gagcagtgga accgggcgtg caggaagata
     2281 tatcccgtcc gggaaataga cggcgacggg ggacaactgt atcacagcag tgtaagcctg
     2341 ctccttgccc atgaggacaa gacctatccc ggcgccttca ttgcgtcgct ttccatcccc
     2401 tggggagagt cgaaagggga cgaggacatg ggagggtatc atctggtctg gacgcgggac
     2461 atggtcaaca cagccatggc tcttttggcc gccggcaacc aggagacacc gctccgggcg
     2521 ctcatctatc tcgccgccac ccagcatgat gacggcggtt tttcccagaa cttctggatc
     2581 aacggggaat cctactggcg cggcatccaa ctggacgagg tcgcgttccc gattcttctg
     2641 gcatggcgtc tctacaggga gaacgccctt gcagagttcg atccctatca catg
//
LOCUS       DASSLD010000641         6591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_895148,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000641 DASSLD010000000
VERSION     DASSLD010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895148"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="VFK23_11565"
     CDS             complement(<1..118)
                     /locus_tag="VFK23_11565"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET7319766.1"
                     /translation="MIDEYQIVLNPVVLGRGKTMFEGVRDRFTLKLTRMRVFG"
     gene            complement(140..370)
                     /locus_tag="VFK23_11570"
     CDS             complement(140..370)
                     /locus_tag="VFK23_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET7319767.1"
                     /translation="MANVKVTIDRDECTSCESCWTICPEVFEQNSGDTWSQIVAKYQV
                     GGDPAVGQFPDTLRDKAQEAADACPVAIIHIG"
     gene            complement(434..1225)
                     /locus_tag="VFK23_11575"
     CDS             complement(434..1225)
                     /locus_tag="VFK23_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HET7319768.1"
                     /translation="MNDRQEKPRTRGLTEIRGPYYTVMGKRYLEDILETMGTYVDSLK
                     FAAGSFSLMSKTVLKELIDLAHKHDVMVSTGGFIEYVLTQGPEVVDKYIKECKNIGFD
                     ILEISGGFVTISTDDWLRLVEKVKKAGLKPKPEVGIQFGAGGATTAEELEAEGTRDPG
                     WAIQQAKRFLDAGAYLIMIESEGITESVKSWRTDVVAKIIDGLGLEKVMFEAADPEVY
                     AWYIKNYGPEVNLFVDHSQIVQCEIYRRGIWGTKSIWGRIITYKG"
     gene            complement(1397..1954)
                     /locus_tag="VFK23_11580"
     CDS             complement(1397..1954)
                     /locus_tag="VFK23_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET7319769.1"
                     /translation="MRRLTVFNLVTLDGYFAGQGGDISWHMVDEEFQELANAASNSGN
                     TLLFGRVTYELMKSYWPTPEALKDDPIVAQGMNKSEKIVFSRSLNKADWNNTRLVKGD
                     MIEEVRKLKQGSGKDLTVLGSGSIVSQLAQAGLIDEYQILLNPVVIGKGKTMFEGVKD
                     RFTLKLTKTRVFGNGNILLTYEPAR"
     gene            complement(2017..5010)
                     /locus_tag="VFK23_11585"
     CDS             complement(2017..5010)
                     /locus_tag="VFK23_11585"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HET7319770.1"
                     /translation="MENSLPQPRIPGSTYRLQFNRFFTFRDAAGVIGYLHDLGVTDIY
                     ASPYVKAKAGSLHGYDITDHNALNPEIGTEEDYAVMTDELKRHGMGQMLDVVPNHMCV
                     DDSANAWWMDVLENGPSSPFAHFFDVDWTPVKKELTDRVLLPILGDQYGNVLEKRELI
                     LCFEEGAFFVNYDEHRFPLRPQSYVSILEHRIELLQELLSAGHPEFSEYQSIITALRH
                     LPPHTETDPVRIEERYREKEVIKRRLRDLCSRCPEVNAHVDENVRLFNGTKGDPKSFD
                     LLDGLLRDQVFRLSHWRVATEEINYRRFFDINELGAIHTERSDVFQATHALVFRLVRA
                     GSVTGLRVDHPDGLYNPAEYLHRLQRGCFVQGMIGPGLSGPSPENERELAERYDEAAL
                     ADPVYKPFYVVGEKILVKSERLPEDWPIFSTTGYVFLNSVNGIFIDGGGAKAFDRIYE
                     KIVKAKVNFQDVVYEKKKLVMQVSMASEANTLGHYLNKLSEKNRHTRDFTLYSLRSAI
                     IEVIACFPVYRTYTNTWAVADRDRLIIESAVSRAKRKNPAISESIFDFLKDVLLLRFP
                     REMTDEDRGAWLDFAMRFQQITGPVAAKGVEDTAFYVYNRLVSLNEVGGAPDRFGTSL
                     EMFHGQNIERGKNWTNALITTSTHDTKRGGDARARINVLSELPAAWAEKVRTWLRMNR
                     KWKTLIEGSEAPDRNEEYLFYQTLIGAWPEGTPEGEGTAYGDFLRRIREYMLKASREA
                     KVNTSWINQNKAYEDALLSFIDAVLRPSAANLFLNDFLPFQRMVSHFGMLNSLSQTLL
                     KICSPGVPDFYQGTELWDYSLVDPDNRRTVDFRKRVRMLAELLRRESETTGVNLAREL
                     SQNKEDGRIKMYLIRKALAFRKVRRELFDQGEYLPLSADGSRAEHVCAFERRRNGDSA
                     IVVVPRFPTRLIAGDGVLPFGKGVWEDSRLVLPGAAPGQRYRNIFTGEIVASAGENGN
                     GGIQLADVFASFPVALLEQTEPVGP"
     gene            complement(5010..>6591)
                     /locus_tag="VFK23_11590"
     CDS             complement(5010..>6591)
                     /locus_tag="VFK23_11590"
                     /inference="COORDINATES: protein motif:HMM:NF023478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HET7319771.1"
                     /translation="LTNYGEYLEKHPPDHEAEIIANSSWSCIHGVERWKSDCGCNSGG
                     HPGWNQQWRAPLRRALDRLRDELAVRFEERARQYLKDPWQARDDYIEVVLDRSPESLY
                     RFFDKHAARMLADGETVKTLKLLELQRHALLMYTSCGWFFDELSGIETVQVMQYAARA
                     IQLWEDLSSENLEPIFLEVLREAKSNVLDHGDGERIYEKFVRPVKVDLKKTAVHYAVS
                     SLFEDYGERAAIYCYDVLQKDYRRADAGRAKLAIGSASVVSSITRESEDVSFAVLHVG
                     SYALNCGVRAFQGDEAYRAMKQEISAVFESGDFAVIVRLMDKHFGVNTYSARDLFRDE
                     QRKVLSLLIGGKLEDYEAAYRRIYDDSRVLMGFLQETGMPLPKAFSAAVEFVLNVDMQ
                     RAFREDKADVEKLRSIVHDMDRWGVEADVNTEFSVRRRLERLMDRLAENSGETGALRG
                     LAEAVGILRSARVDIVYWKVQNIYFRMAKTAYPGFAARSSAGDRAAQEWVGTFRDLGE
                     QLFFNTGAVLRKAEGEKT"
BASE COUNT         1222 a         2061 c         1829 g         1479 t
ORIGIN      
        1 tgccgaacac ccgcatcctg gtgagcttca gcgtgaagcg gtctctcacg ccttcgaaca
       61 tggtcttccc cctcccaagg accacggggt tcagcacgat ctgatactcg tcgatcagac
      121 ccgcctgtgc cggctgcgat catcctatat gaatgatagc gaccgggcag gcgtccgcgg
      181 cttcctgagc cttgtcccgc aaagtatctg gaaactggcc gactgccgga tcacctccga
      241 cctgatattt cgcaactatc tggctccacg tatcccccga gttctgctca aagacctccg
      301 ggcagatcgt ccagcatgat tcacagctcg tgcactcatc cctgtctatc gttaccttta
      361 cgtttgccat tacaacacct cccgtggttg aatctattct cggtcctttt gattcggccc
      421 cttctagaag ctcttatccc ttgtacgtaa tgatgcggcc ccatatgctc ttcgtgcccc
      481 agatgccgcg acggtagatc tcgcactgga cgatctgact gtggtcaacg aaaaggttca
      541 cttcaggtcc gtagttcttg atgtaccaag cgtagacctc gggatcggct gcctcaaaca
      601 ttaccttctc aagcccgagc ccatcgatga tctttgcgac aacatcggtc cgccatgatt
      661 tcacgctctc ggtgattccc tcagactcga tcatgatgag ataagcgccg gcatcaagga
      721 atcgctttgc ctgctggatg gcccatccgg ggtcccttgt gccttccgct tcaagttctt
      781 cggcagttgt cgcccctcca gcgccgaatt ggatgcccac ttcgggtttt ggcttgagtc
      841 ccgctttttt tactttctcg acgagtctga gccagtcgtc ggtcgagata gttacgaaac
      901 cgcccgatat ttccagtatg tcaaacccga tgttcttgca ctctttaatg tacttgtcga
      961 cgacctcagg cccctgggtg aggacgtatt cgatgaaccc gccggtcgaa accatgacgt
     1021 catgtttatg cgccaggtca ataagctcct tgagaaccgt cttcgacatc aggctaaaag
     1081 acccggcggc aaatttgagc gagtctacat acgtccccat ggtttcaaga atatcttcga
     1141 ggtagcgctt tcccatgacc gtgtagtagg ggcctcggat ctcggtcagc ccgcgagtcc
     1201 tcggtttttc ctgccggtcg ttcatacgaa taaagtcaaa tgctcgttcg gactttgaca
     1261 tcccagacat tgtttgcacc tccgtttttt gaaggaacct tcccaggaat ctgccacatt
     1321 gatctcctca tagatggacc gttatattac agggaagtgc atcagggatc gtgaatcgcc
     1381 cgtcatagac cctcccttac cgcgccggtt cgtaggtcag caggatgttc ccgttgccga
     1441 acacccgcgt cttggtgagc tttagcgtga aacggtcttt cacgccttcg aacatggtct
     1501 ttcccttccc gatgaccacg gggttcagca ggatctgata ctcgtcgatc agacccgcct
     1561 gcgccagttg cgatacgatg ctgccgcttc cgagcactgt caggtccttt cccgacccct
     1621 gcttcagctt ccggacttcc tcaatcatgt cgcccttcac cagcctcgta ttgttccagt
     1681 ccgccttgtt cagggaccgg gaaaagacga tcttctcgga cttgttcatg ccctgggcca
     1741 cgatgggatc gtccttgagc gcttccggcg ttggccagta gctcttcatg agctcgtagg
     1801 tgacccgtcc gaacagcagc gtgttgcccg aattcgacgc cgcatttgcc agttcctgga
     1861 attcttcgtc caccatatgc caggaaatgt cgccaccctg gcccgcaaag tacccgtcca
     1921 gcgtcaccag attgaatacc gtcaatcttc gcatcgttgt ctcctttcct gcgccggctt
     1981 cgacatcctt cccttcatga gaacaaagcc ggtgcgctac ggccccactg gctctgtctg
     2041 ttcaagaagc gcaacgggaa aactcgcgaa cacatcagcg agttgtattc cgccgttccc
     2101 gttttctcct gctgaggcga cgatctcgcc ggtaaagatg ttccggtacc gctgccccgg
     2161 cgctgcaccc ggcagaacaa gccgggaatc ctcccaaaca cccttgccga agggcagcac
     2221 tccatctccg gcgatcaacc gggtcggaaa acggggcacc acgactatcg ccgaatcgcc
     2281 gttccgtctc cgttcgaacg cgcacacatg ctccgcccgc gacccgtcgg cagacaaagg
     2341 cagatactcc ccctgatcga acaattcccg gcgcactttc cggaaggcga gtgctttgcg
     2401 gatgagatac atcttgattc gcccatcttc tttgttctgc gacaattccc gggcaagatt
     2461 cacgccggtc gtctccgact cccgccgaag aagctccgcg agcatgcgga cgcgtttcct
     2521 gaagtccacg gtcctccggt tgtcggggtc cacaaggcta tagtcccaca gctccgtccc
     2581 ctggtaaaaa tcgggaacgc cgggagaaca gatcttgagc agcgtctggg acagggagtt
     2641 gagcatgccg aaatgggaca ccatgcgctg gaagggcagg aagtcgttga gaaagagatt
     2701 tgccgcggaa gggcgaagca cggcgtcgat gaaggagagc agcgcgtcct cgtaggcctt
     2761 gttctggttt atccagcttg tgttcacctt cgcttcgcgc gacgccttga gcatgtactc
     2821 cctgatgcgc cggagaaaat ctccatacgc ggtcccctca ccttccggcg tcccctccgg
     2881 ccacgcgccg atgagggttt ggtaaaacag atattcttca ttccggtcgg gcgcctcgga
     2941 gccctcgatc aacgttttcc acttcctgtt catccgcaac catgtccgga ccttctccgc
     3001 ccacgccgct ggcaactcgg agagcacgtt gatccgggcg cgggcgtctc cgccgcgttt
     3061 ggtgtcatgg gtcgatgtcg tgatgagcgc gttcgtccag ttcttgccgc gttcgatgtt
     3121 ctggccgtgg aacatctcga gcgacgtgcc gaaccggtcc ggggccccgc ccacttcgtt
     3181 caaggacacg aggcggttgt atacgtagaa agccgtgtcc tcgacgcctt tcgccgcgac
     3241 cggtccggtg atctgctgga accgcatggc gaagtcgagc caggcgcccc tgtcctcgtc
     3301 ggtcatttcc cgggggaacc ggagcaggag cacgtccttc agaaaatcga agatggattc
     3361 gctgatggcg ggatttttcc tcttcgcccg cgagaccgcc gactcgatga tcaggcggtc
     3421 acggtctgcg acggcccagg tgttcgtgta ggtgcggtag accgggaagc aggctatcac
     3481 ctcgatgatt gcgctccgga gactgtagag cgtgaagtcc cgcgtgtgac ggtttttttc
     3541 cgagagcttg ttcaggtagt gaccgagggt gttcgcctcg cttgccatgg acacctgcat
     3601 gaccagtttc ttcttttcgt atacgacgtc ctggaaattc accttcgctt tgacgatctt
     3661 ttcatagata cggtcaaagg ccttcgctcc gccgccgtcg atgaagatcc cgttcacgga
     3721 attaaggaac acgtagcccg tggtgctgaa aataggccag tcttcgggca ggcgttcgct
     3781 ctttaccagg atcttctctc ccacgacata gaagggctta tacacgggat cggcgagcgc
     3841 cgcctcgtca tatcgttccg ccagctcccg ctcgttctcc ggggatggac cggacagtcc
     3901 cggcccgatc atcccctgca cgaagcagcc gcgctgcagc cggtggaggt actccgccgg
     3961 attgtagagc ccgtcgggat ggtcgaccct gagtccggtg acgcttcccg cccgaacgag
     4021 ccggaacacg agggcatggg tggcctggaa aacatcggaa cgctccgtat ggatcgcgcc
     4081 cagttcgttg atgtcgaaaa accgccggta gttgatctcc tccgtggcca cacgccagtg
     4141 ggagagccgg aacacctggt cgcgcaggag tccgtcgagg aggtcgaaac tcttcgggtc
     4201 ccctttcgtt ccgttgaaca gccggacgtt ttcatccaca tgcgcattga cctcgggaca
     4261 gcgggagcac aggtcccgga ggcgccgctt gatcacctcc ttttcgcggt accgctcctc
     4321 gatccggaca ggatcggttt ccgtatgggg cggaagatgc cggagcgccg tgatgatgct
     4381 ctggtactcg ctgaattcgg gatggcctgc ggagagcagt tcctgaagaa gctcgatgcg
     4441 gtgttccagg atggagacat agctctgggg acggagggga aaccggtgtt cgtcgtagtt
     4501 cacgaagaac gcgccctcct cgaagcaaag gataagctcc cgcttttcca ggacgtttcc
     4561 gtattgatca ccgaggatgg gcaggaggac cctgtccgtc agctcctttt tgaccggcgt
     4621 ccagtcgacg tcgaaaaaat gcgcaaaggg agagctcggc ccgttttcga gcacgtccat
     4681 ccaccatgcg ttggcggagt catcgacgca catgtggttc ggaacgacat cgagcatctg
     4741 gcccatgccg tggcgcttca gttcgtccgt catcacggcg tagtcctcct ccgtgccgat
     4801 ctccgggttc agggcattgt ggtccgtgat gtcgtagccg tggaggcttc cggctttcgc
     4861 cttcacatac ggcgaggcgt agatgtccgt gacgccgagg tcgtgaagat agccgatgac
     4921 ccccgccgcg tcgcggaacg tgaagaatcg gttgaactgg agccggtagg tggagccggg
     4981 aattcggggc tgcgggagac tgttctccat cacgtttttt ctccttcggc cttcctgagc
     5041 accgcgccgg tgttgaagaa gagctgttca ccgaggtccc tgaaagtgcc gacccactcc
     5101 tgagccgccc tatcgcccgc agaggagcgg gcggcgaatc cggggtatgc cgttttcgcc
     5161 atgcggaagt agatattctg gaccttccag tacacgatat ccacgcgcgc ggacctgagg
     5221 atccccacgg cttctgcgag cccgcgcagc gcgcccgtct cgccggaatt ctccgcgaga
     5281 cggtccatca gccgctcgag acggcgccga acgctaaatt cggtgttcac gtccgcttcc
     5341 acgccccatc ggtccatatc atggacgatc gacctgagct tttccacgtc ggccttgtcc
     5401 tccctgaacg ccctctgcat gtccacgttc aggacgaatt caaccgcggc cgagaacgct
     5461 ttcggaaggg gcatccccgt ctcctgcaga aaccccatga ggacccggct gtcgtcgtat
     5521 atccggcggt atgccgcctc atagtcctcc agcttgccgc cgatgagcag gctgagcacc
     5581 tttcgctgtt catcccggaa gaggtcgcgg gccgaatagg tattcacgcc gaaatgcttg
     5641 tccatcagcc tgacgatgac cgcgaagtcg ccggactcga agaccgcgga gatctcctgc
     5701 ttcatggctc ggtacgcctc gtcgccctgg aacgcgcgca cgccgcagtt cagtgcgtag
     5761 ctgccgacat ggaggactgc gaaactgacg tcttcggact cccgggtgat gctcgagacg
     5821 accgaggcgc tgccgatggc cagtttcgcc cttcccgcgt ccgccctccg gtagtccttc
     5881 tgcaggacgt cgtagcagta gatggctgcg cgttcgccgt aatcctcgaa cagggaactc
     5941 accgcgtagt ggaccgccgt tttcttgagg tccaccttca cgggcctgac gaacttctcg
     6001 tagatgcgct ccccgtcccc gtgatcgaga acattactct ttgcttccct caggacctcg
     6061 agaaatatcg gttccaggtt ttcactggaa agatcttccc agagctggat cgcccgggcc
     6121 gcatactgca tgacctgcac ggtctcgatg cccgaaagtt cgtcgaagaa ccatccgcag
     6181 ctcgtgtaca tgagcagggc gtggcgctgc agttcgagca gcttgagcgt tttcaccgtt
     6241 tccccgtcgg caagcatcct cgcagcgtgt ttatcgaaga aacggtagag gctttccggc
     6301 gagcggtcga ggaccacctc gatgtagtcg tcccgcgcct gccagggatc tttcagatat
     6361 tgccttgccc gctcttcaaa gcggaccgca agctcgtccc ggagccggtc gagagcccgg
     6421 cgcagcggcg cacgccactg ctggttccag ccgggatgtc ccccggagtt gcagccgcag
     6481 tcgcttttcc agcgctcgac gccgtggatg cagctccacg acgaattcgc gatgatctcc
     6541 gcctcgtggt ccggcgggtg cttttcgaga tactctccgt aattcgtgag c
//
LOCUS       DASSLD010000642         2607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_310161,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000642 DASSLD010000000
VERSION     DASSLD010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310161"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VFK23_11595"
     CDS             <1..464
                     /locus_tag="VFK23_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HET7319772.1"
                     /translation="PEYGPERAGAPLRAYNRISEKALRMHCPVLNPGVVAVVDATLID
                     AINVAEGAKDDAVFVVNTARDPKEVREKLKAKPSQKVFTVDATKIAIETIGRPMPNSC
                     MLGAVNRATAIVPMDVLLDDVKGSFGKKFAQKIIDGNLTAVKRGYEEVKEG"
     gene            461..754
                     /locus_tag="VFK23_11600"
     CDS             461..754
                     /locus_tag="VFK23_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HET7319773.1"
                     /translation="MKQMNWKDLPHAGTILKAGNADEYNTGSWRSLKPRWVEENCIQC
                     LFCWIYCPDSAVNVKDEKMAGFDYDHCKGCGVCAMECPGKKGNKAIVMEEEGK"
     gene            756..1946
                     /gene="porA"
                     /locus_tag="VFK23_11605"
     CDS             756..1946
                     /gene="porA"
                     /locus_tag="VFK23_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HET7319774.1"
                     /translation="MGTTTTKTVAVTGNQAVAEALRQLNPDVMPAYPITPSTGIMESF
                     TSFVSNGKVDTETILVESEHSAMSACIGASAAGGRVATATSAQGLALMWEMLHIASGY
                     RLPILMALVNRALSAPLNIHGDHSDGMGMRDCGWIQLFSENAQEAYDNTIMAYRIAEH
                     KDVRLPIAVNMDGFIISHSIESMTFIPDEEVKKFVGDYIAIDPLLDLDHPKSIGALVL
                     PDYYMEHKRAQHEAMLNAKKIILDVSAEFANLFGRQYDLFESYRLEDAEVVLVALNSA
                     AGTIKDEVDRYREKGVKAGLLKPRVFRPFPYEEIGAALKNAKAVSVMDRADSYGGYGP
                     LFMEIAPAVYPYRQGPVLFNKIYGLGGRDLMPLDVDQVISETIEVAKTGKVKMVKEYI
                     TIRN"
     gene            2065..>2607
                     /locus_tag="VFK23_11610"
     CDS             2065..>2607
                     /locus_tag="VFK23_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7319775.1"
                     /translation="MSTTQEKPLTLKSLTHKPVMLTGGHRMCSGCGAPTIVRQVLMAI
                     DEPVVIANATGCLEVSTCLFPYTAWQVPWMHSAFENAAATASGIETMYRSLRKQGKLK
                     KEMKFIAFGGDGGTYDIGFQALSGAMERGHQLLYICYDNGAYMNTGIQRSSATPFGAD
                     TTTQPVGSKIPGKSVRRKDLT"
BASE COUNT          623 a          739 c          762 g          483 t
ORIGIN      
        1 ttccggagta cggacccgag cgagcgggag ctccgctccg cgcttataac cgcatcagcg
       61 agaaggcgct ccgcatgcat tgccccgtgc tgaaccccgg cgtggtggcg gtcgtggacg
      121 caacgctcat tgatgcgatc aacgtggccg agggggcgaa ggacgacgcc gtcttcgtcg
      181 tgaacaccgc cagggacccg aaggaggtgc gggagaaact gaaggcgaag ccctcgcaga
      241 aggtcttcac cgtggacgcg accaaaatcg caatcgagac catcggcagg cccatgccga
      301 actcctgtat gctcggcgcg gtgaacaggg cgacggcaat tgtacctatg gacgtgctgc
      361 tcgatgatgt gaagggcagc ttcggcaaga agttcgcgca gaagatcatc gacggcaatc
      421 ttactgcggt gaagcgcgga tatgaggagg taaaagaggg atgaaacaga tgaactggaa
      481 agacctgccg catgccggca cgatcctgaa ggccgggaat gcggatgaat ataatacagg
      541 ctcgtggcgg tccctgaagc cgcgctgggt tgaagagaac tgcattcaat gtctattttg
      601 ctggatctac tgccccgatt ctgcagtcaa cgtcaaagac gaaaagatgg ccggcttcga
      661 ctatgatcac tgcaagggct gcggagtgtg tgccatggaa tgcccgggga aaaaaggcaa
      721 taaagccatc gtcatggaag aggagggcaa gtaacatggg aacaacgacg accaagaccg
      781 tggcagtcac cggtaaccag gcagttgcag aagcgcttcg ccagttaaat cccgacgtga
      841 tgccggccta tcccataacg ccgtcgacgg gcatcatgga atcgttcacc tctttcgttt
      901 cgaacgggaa ggtggacacc gagacgatac tcgtggagag cgagcacagc gccatgagcg
      961 cctgcatcgg cgcctcggca gccggcggac gtgttgcaac agcaacttct gcgcagggcc
     1021 tcgccctcat gtgggagatg ctgcatatcg cttccggcta tcgcttgccg atcctaatgg
     1081 ccctcgtgaa ccgggcgctg tcagcgccgc tcaacatcca tggcgaccat tcagacggca
     1141 tgggcatgcg cgactgcggc tggatacagc tcttttccga gaacgcccag gaagcgtacg
     1201 acaatacgat catggcgtac cgcatagccg aacataagga tgtgcggctg ccgatcgcgg
     1261 tcaacatgga cggcttcatc atcagccact ctatcgagag catgacgttc atccccgatg
     1321 aggaagtgaa aaaattcgtg ggcgattata tcgcgatcga ccccctcctc gacctagatc
     1381 accccaagag catcggcgca cttgtgctgc cggactacta catggagcac aaacgcgccc
     1441 agcacgaagc catgctcaat gcgaagaaaa tcatattgga tgtatctgcg gagttcgcca
     1501 acctttttgg ccgccaatat gatctcttcg aatcctaccg gcttgaagat gccgaggtcg
     1561 tcctcgttgc gctcaactcc gcagcgggca cgatcaaaga cgaggtcgac cgctaccgtg
     1621 aaaagggcgt gaaggcaggc ttgctcaagc cccgcgtgtt cagaccgttt ccgtacgagg
     1681 aaatcggcgc tgctctcaag aacgccaagg ccgtctccgt catggaccgg gcggattcct
     1741 acggcggcta cggccccctc ttcatggaaa tcgccccggc cgtctacccc tatcgtcagg
     1801 gaccggtgct gttcaataag atctacggcc tcggcggcag ggacctgatg ccgctcgatg
     1861 tagaccaggt gatcagtgag accatagagg tcgctaagac gggtaaggtg aagatggtga
     1921 aagaatatat taccatcaga aactaatatt ttttttccca cgaggacgca gacaaaacca
     1981 taatatcctt ttgacaggat tgcaggatag acgacaaaaa aagattttac gctgttatcc
     2041 tgtctaagaa tctaaggaga gaacatgagt acaacacagg aaaaacccct gaccctgaaa
     2101 tcattgacgc acaagccggt catgcttacc ggcggccacc ggatgtgttc cggctgcggc
     2161 gcccctacca tcgttcgcca ggtccttatg gccatcgacg aaccggtggt gattgcgaac
     2221 gccacaggct gcctcgaggt ctcgacctgc ctgttcccgt atactgcctg gcaggtaccg
     2281 tggatgcaca gcgccttcga gaacgcggcg gccacggcct cggggatcga gaccatgtac
     2341 cgttcgctcc gtaaacaggg aaagctcaag aaggagatga agtttatcgc tttcggcggc
     2401 gacggcggca cctatgacat cggttttcag gccctgtcgg gagccatgga gcgcggtcat
     2461 cagctgcttt acatctgcta cgacaacggc gcctacatga atacgggcat ccagcgctcg
     2521 agcgcgaccc cattcggggc cgataccacg acacagccgg tggggtccaa gatccccggc
     2581 aagtcggtgc gccggaagga cctgacc
//
LOCUS       DASSLD010000643         3466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1070665, whole genome shotgun sequence.
ACCESSION   DASSLD010000643 DASSLD010000000
VERSION     DASSLD010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070665"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..277)
                     /locus_tag="VFK23_11615"
     CDS             complement(50..277)
                     /locus_tag="VFK23_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319776.1"
                     /translation="MTLYCESYEKIVPDLTETKYWERREAKSAAFLLVIPGSTIMGFG
                     FGLLSGHTTPSAIIGFGAGLLIWGLIVALTK"
     gene            complement(488..1054)
                     /locus_tag="VFK23_11620"
     CDS             complement(488..1054)
                     /locus_tag="VFK23_11620"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7319777.1"
                     /translation="MMSGATQGRLLIAMAGLPGTGKSTIASLLAERLHALVLNKDAIR
                     ARLFSGTAIDFSRAQDDLCMEVIFIAAEYVLRANAKQTIIIDGRTFSQSYQVEHLFAR
                     AGSLNVKPAVIECVCSDTVARQRLDEAQRTGAHPACNRTYQLYLALKERADPLTADRL
                     VIDTGREPVEKSVERCVAYLRGLGHRAP"
     gene            complement(1063..3129)
                     /locus_tag="VFK23_11625"
     CDS             complement(1063..3129)
                     /locus_tag="VFK23_11625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7319778.1"
                     /translation="MSRVLIVDDILENRYMLETLLKGSGYDVFAAVNGAEALDLARKS
                     PPDLVITDILMPVMDGFTLCRQWKADSRLRHIPFVFYTATYTEPKDEALALSLGAERF
                     IIKPKKPEEMIALLREVLGDADAGRLVPCDSPLKGEKEFLQDHDAAVSRKLDRKMAQL
                     EQTNAALQEEIAERKRIESELLYRNTILATQQETSLDAILVVDDRGKMISFNRRFVDL
                     WGIPAAVIDKKSDKKALQAVLDKVMDPKGFLEKVNELYEHKDRTSRDLVTLRDGRILD
                     RYSSPVIEKNGQYRGRVWYFRDITEQRKLEQQLLQAQKMEAIGLLAGGIAHDFNNILT
                     AIIGFGSVLEKEIPPDDPMQDRVKHILEAGARAAQLTRSLLAFSRKQVLYMRPTTLNS
                     IIVGEEKFLRRIIGEDIDMKTILHRDPVILADSGQIEQILLNLATNARDAMPRGGSLT
                     VETDVVTMTEAFVNAHGFGAPGAYATISVTDTGVGMDEITRQRMFDPFFTTKEVGYGT
                     GLGMAIIYGIVKQHKGYINVYSQIGMGTTVRIYIPVHQEQAEPQKSVQSAPPVLTGTE
                     TVLLAEDDATLRSFFRDILTKYGYTVIAAENGTDAISRFTEQKDSIQLVIVDMVMPKK
                     NGREVYEEVRRLKPGVKVIFSSGYAADRVLQEGFPAGSEFIAKPASPHDYLRKIRDVL
                     DGPRAA"
     gene            complement(3126..>3466)
                     /locus_tag="VFK23_11630"
     CDS             complement(3126..>3466)
                     /locus_tag="VFK23_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7319779.1"
                     /translation="YLVTYILEKNGYEVVGARDGQQGIAAASRELPALILLDIQLPGM
                     DGYAVAKKLRDRPELAGIPIVAVTSYAMVGDRERALAAGCTGYIEKPINPETFLTQIE
                     RHLPSGGPLP"
BASE COUNT          647 a         1026 c          985 g          808 t
ORIGIN      
        1 taatctcgca aaatgaagaa tcgggcgcgc ctggaaaaga cggcacgtgc tacttggtca
       61 acgcaacgat caatccccaa atgaggagac ccgctccgaa gccgataatt gcgctcggag
      121 tcgtatgacc cgagagcagg ccgaagccaa accccatgat ggtgctgccg ggaatgacga
      181 gcagaaaagc agctgatttc gcctctcgtc gctcccaata tttcgtctcg gtcaggtccg
      241 gaacgatctt ctcgtatgat tcgcagtaca gtgtcatgac agctcctcac cctccttttc
      301 acgcgcaccg aacatccttg gcgccctgtc cgtttttgag acagggcacg ctgttcggtt
      361 ctttgttttc tgcgtttagg acgattggga aagggaaagg ttacgaggga aaatataagc
      421 ggaagattta tactacgtta gtatgagaaa aagagctttc ggtgagcaag tttggcaagt
      481 cacgtcctca cggcgcgcgg tgaccgagcc ccctcagata tgccacgcac cgttctaccg
      541 atttctctac gggttcccgg ccggtatcaa tgaccagccg atccgcggtc agcgggtcgg
      601 ccctttcctt taacgccagg tataattggt aggtccggtt gcaggccgga tgggcgcccg
      661 tacgctgagc ctcgtcgagc ctctgcctgg cgacggtgtc gctacagacg cattcgatga
      721 cggcgggttt tacgtttaaa gatccggccc gcgcaaagag gtgctctacc tggtaggatt
      781 gtgaaaaggt tctcccgtcg atgataatgg tttgcttcgc atttgcccgc aggacgtact
      841 ccgctgcgat aaatatgacc tccatgcaga gatcgtcctg cgctcgggaa aaatcgatcg
      901 ctgtgcctga aaataatctc gcgcggattg cgtctttatt gagcacgagg gcgtgaagac
      961 gctctgcgag cagagatgcg atcgtgctct tgccggtacc cggcagccct gccatggcga
     1021 tcaacaaccg gccctgtgtc gcaccgctca tcatggccgg actcaggccg ctctggggcc
     1081 gtcgagcaca tcacggatct tcctgagata gtcatgcggc gatgccggct ttgcgatgaa
     1141 ctcgctcccc gcggggaacc cttcctgaag caccctgtcc gcagcgtagc cgcttgaaaa
     1201 gatcactttg actccgggtt tcagccgccg gacctcctcg tacacctctc ggccgttttt
     1261 tttcggcatg accatgtcga caatgacgag ctggatgctg tccttctgct cggtgaacct
     1321 gctgattgcg tcggtcccgt tttccgcggc gataacggtg tagccgtatt tggtcaggat
     1381 gtccctgaag aagcttcgca acgtcgcgtc gtcctctgcg agcagaacgg tttcggttcc
     1441 ggtcagtacc ggcggggcag actgaaccga tttctgaggt tccgcctgtt cctggtggac
     1501 ggggatatag atcctgacag tcgttcccat gccgatctgg ctgtagacat taatataacc
     1561 cttatgctgc ttgacgatcc cgtagatgat ggccatgccc agtcccgtgc cgtagccgac
     1621 ctccttggtc gtgaaaaacg gatcgaacat gcgttgccgt gttatttcgt ccatgccgac
     1681 gccggtatcg gtgacggaga tcgtcgcata ggcgccgggg gcgccgaacc catgggcgtt
     1741 tacgaacgcc tcggtcatgg tcaccacgtc cgtttcgact gtcagggatc ctcccctcgg
     1801 catagcgtcg cgggcgttcg tcgccaggtt caacaggatc tgttcgatct ggccgctgtc
     1861 ggcgaggatg acggggtccc ggtgcaggat cgttttcatg tcgatgtcct ctccgataat
     1921 cctgcgcagg aacttctctt ccccgacaat gatgctgtta agcgtcgtcg gcctcatgta
     1981 caggacctgc tttctgctga atgcgagcag gctgcgggtc agctgggccg cccgtgcccc
     2041 cgcttcgagg atatgcttca cgcggtcctg cattggatcg tctggcggga tctccttttc
     2101 cagaacgctt ccgaagccga tgatagccgt gagaatattg ttgaagtcgt gagcaatgcc
     2161 gccggccaaa aggccgatgg cctccatttt ttgggcctgc agaagctgtt gctcgagctt
     2221 tctctgttcg gtgatgtccc ggaagtacca cacccttcca cggtactgcc cgtttttctc
     2281 tatgacgggc gaagagtacc ggtccagaat gcggccatcc cgcagcgtga cgaggtccct
     2341 gctcgttcgg tccttgtgtt cgtacagctc gttcaccttt tccaggaagc ccttcgggtc
     2401 catgaccttg tccaggaccg cttgcagcgc tttcttgtcc gacttcttat cgatgacagc
     2461 ggccggtatc ccccagaggt cgacaaagcg cctgttgaag gaaatcatct tcccccggtc
     2521 gtcaacgacg aggatcgcat cgagggaggt ttcctgctgg gtcgcgagaa tcgtgttccg
     2581 gtagaggagt tcgctttcga tccgcttccg ttcggcgatc tcctcctgca gggccgcatt
     2641 cgtctgttcc agctgcgcca ttttcctgtc gagctttcgg gacacggctg cgtcatggtc
     2701 ctgcaggaac tccttttccc ctttgagcgg cgaatcgcag ggaaccagcc tgcccgcgtc
     2761 ggcatctccc agcacctctc gcaggagcgc tatcatttct tcgggctttt tgggtttgat
     2821 gatgaaacgt tccgcgccca gactcagggc aagggcctcg tccttcggct cggtataggt
     2881 tgccgtataa aaaacaaagg ggatatgcct cagccgggaa tccgccttcc actgccggca
     2941 cagggtaaaa ccgtccataa ccggcatgag aatgtccgtg atgacgaggt ccggcgggga
     3001 cttccgggcg aggtcgaggg cctcggcacc gttcacggcc gcgaacacgt catacccgct
     3061 tcccttcagg agtgtttcaa gcatgtaccg gttttccaaa atatcatcca cgatcagcac
     3121 acggctcatg gcaaaggtcc tcctgacggc aggtgccgtt cgatctgggt gagaaaggtc
     3181 tcgggattga tcggtttttc tatatacccg gtgcaccccg ctgccagggc ccgctcccgg
     3241 tctcctacca tcgcgtagga ggtcacggcc acaatgggaa tgccggcgag ctccggtctg
     3301 tcgcgcagtt ttttcgcaac cgcatagccg tccatcccgg gcagctggat gtccagcaga
     3361 atgagcgccg gcagctcccg tgacgctgcg gctatcccct gctgtccgtc ccgggcgccg
     3421 acgacctcgt agccgttctt ctcgagaata tacgtcacga gataga
//
LOCUS       DASSLD010000644         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1199501, whole genome shotgun sequence.
ACCESSION   DASSLD010000644 DASSLD010000000
VERSION     DASSLD010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199501"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /gene="queD"
                     /locus_tag="VFK23_11635"
     CDS             <1..379
                     /gene="queD"
                     /locus_tag="VFK23_11635"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HET7319780.1"
                     /translation="SNAMYELMVESKFSAAHQLRGYQGQCEKLHGHNWRVTVAVTAER
                     LDEQGLAIDFHQLKKALREILDQLEHTFLNDIFPFTQINPSSENLAKWIYDNMMKKVN
                     DDNIEVASVTVWESDTASASFFE"
     gene            500..1837
                     /locus_tag="VFK23_11640"
     CDS             500..1837
                     /locus_tag="VFK23_11640"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HET7319781.1"
                     /translation="MDKLIAEKILSLASSLKVDSSDVYLRSHTSTSIEVKDQAVDAFD
                     RARDIGAGLRVLVGGRLGFAFTTDLSDSALASLARAAVTNARNIEPDEHHFIPERPSA
                     PYQSVGIYDPEIPALTEKDKIDRVMAMEREAFAVDPRIKRIRKAQAAFSDSETLILNT
                     KGAEVSYKGTACSSSIEVVAEDKGESQAGSEFDVNRFYRRLEIEEVGRKAARRALDLL
                     GARHIDSVKAPVVLEAPVAQEFLSLMAGGFSAESVQKKRSLFIGKLDREVASPLITVH
                     DDGLLEGGLGTAPSDDETVPAKKKTVIAKGRLAMFLYNVYTARKDKTESTGNGMRGGF
                     KGLPGVGVTNLYLEPGTGTLEDLIASVDRGLLVTDVMGMHTANSISGDFSVGAAGFWI
                     EKGKKAYPVREIAIAGNILDVMKNVDAVGGDLRFSGRIGSPSLRIKELSIGGK"
BASE COUNT          597 a          732 c          722 g          557 t
ORIGIN      
        1 atccaacgct atgtatgagc ttatggtcga atccaaattc tccgcggcac atcagctccg
       61 cggctatcag ggccagtgcg agaagctcca cggccacaac tggcgcgtca cagtcgccgt
      121 gacggcggaa cgcctggacg agcaggggct ggccattgac tttcatcaac tgaagaaagc
      181 gctccgcgaa atactcgacc agttggaaca cacattcttg aacgatatat ttcctttcac
      241 gcagatcaac ccctcatcgg aaaaccttgc caaatggatc tatgacaaca tgatgaaaaa
      301 ggtcaatgac gataatatcg aagtcgccag cgtgaccgtc tgggaatcgg acacggcttc
      361 ggcgagcttt ttcgaataaa aaaacctttc tgccacagag gccactgagg gaaaccttaa
      421 gattccaaat tcaagattca agattttctc tgtgatcgct gtgcctctgt ggctgaatta
      481 ttttcttcgg ggacctgaat tggataaact catcgccgaa aaaatactct cgcttgcctc
      541 ctcgctcaag gtcgattcat ctgacgttta cctccgttcc catacgtcca cctcgatcga
      601 agtaaaggat caggcagtcg acgcctttga ccgcgccagg gacatcggcg cggggctgcg
      661 cgttctcgtt ggcgggagac tgggatttgc ctttacgaca gacctgtctg acagcgcgct
      721 cgcttccctt gcccgggccg cggtcacgaa cgcaaggaac atcgaaccag acgaacacca
      781 cttcattccg gaaaggccct ctgccccata ccaatccgtc ggcatctacg atcccgagat
      841 ccccgcgctg actgagaagg acaagatcga ccgcgtcatg gccatggaac gggaggcctt
      901 cgcggtggac cccaggatca aacgcatccg taaagcccag gcggccttct ctgattcaga
      961 aaccctgatc ctgaacacga agggcgctga ggtctcctac aagggcacgg cctgctcgtc
     1021 aagcatcgag gtggtcgccg aggacaaagg cgaatcgcag gccggttcgg agttcgatgt
     1081 gaaccgcttt taccggaggc tcgaaatcga ggaggtcgga cgcaaggccg ccagaagggc
     1141 gctcgacctg ctgggggcga ggcacatcga ctcggtcaaa gcgccggtgg tcctggaggc
     1201 cccggtggcc caggaattcc tctccctgat ggccggcggg ttttcggccg agagcgtaca
     1261 gaagaaaagg tcgcttttca tcggcaagtt ggaccgggaa gtcgcgtcgc cgctcatcac
     1321 ggtccatgac gacggccttc tcgagggagg cctgggaacc gcgccgtccg acgacgagac
     1381 cgtgccggca aaaaagaaaa cggtcatcgc aaagggcagg ctcgccatgt tcctctacaa
     1441 tgtctacacg gcgcgcaagg acaagaccga atcaacggga aacggcatgc ggggcggttt
     1501 caagggactt cccggcgtgg gcgtgacgaa tctttacctc gagccgggca cgggtacgct
     1561 tgaggacctc atcgcgtccg ttgatagggg gctcctggtg accgacgtga tgggcatgca
     1621 caccgccaac tccatatccg gagacttctc ggtcggggct gccggattct ggatcgagaa
     1681 aggcaagaag gcctaccccg tgcgtgagat cgccatcgcc gggaacatcc tcgacgtcat
     1741 gaagaacgtc gatgccgtgg gcggcgatct gcggttctcc ggaaggatcg gaagcccgtc
     1801 gctccggatc aaagagcttt cgatcggggg gaagtaagga cttcatccca aagttgttat
     1861 agttttgttc gatggaaaca ccggcagttc cctcagtaat tgtccgagca tacccacgaa
     1921 atgtccatct taatcggcac ttgacctcac ctatccgatc ctctatgctt tatatactat
     1981 catcgacaag gttcttcctg ctggctgatc gcgctctctc atcgtaccga gttgtaatca
     2041 gccgttctca ctaatgagta tactgaggcc tatgtgccgg ggagggggac atgaaacacg
     2101 agatcctggt gattgccggg gtattagctg ccctggtctt tttccgggcg cctgcctacg
     2161 gcgaggatct cgagaaaagc aagttctgcg accaaaagac gtttgcgaac agcataaatt
     2221 tgaagtttat ctggattaag accctctaca caacagtctt tttgccgaag aagccgatat
     2281 atataatgtt tgggaaaaaa ggatcggctt cgggatcgac actactcttg ccagcggcaa
     2341 ttttttcgac gtcaggccct acgcaacatt ttcaaacggc cagctgccgg ccatttttct
     2401 tgtcggatac gaggccgcca gtaccggcgc aagattcgtt gattacggca tgtggtatac
     2461 gctcaaggcc cgtgcgctcg acatcctgct tggcgcggtt aatttcagcg ccgtttctcc
     2521 ggaagccagg gactatctcg attcctttgc cggtatgact gcgcccttgg gcgcatattc
     2581 ggtcggcatc gatttggaag agatccat
//
LOCUS       DASSLD010000645         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_994170,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000645 DASSLD010000000
VERSION     DASSLD010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994170"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            445..1329
                     /locus_tag="VFK23_11645"
     CDS             445..1329
                     /locus_tag="VFK23_11645"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET7319782.1"
                     /translation="MLNLIGGTPLVRISRVTKHLNPQVEVFAKLERGNPGGSVKDRAA
                     YRMIADAEEAGLLTRDKIILDSTSGNTGIAYAMIGAFKGYRVRIVIPENASVERKKII
                     SGFGAELIYSSPFDGADGAILLARELIRTHPDLYYQPDQYNNPSNGKAHELTTGPEII
                     RQSGGRVTHFVACVGTGGTIMGTGRALRKYNPSIRIVSVHPDDAMHGIEGTKYLGEGA
                     IVPGIFDTDFPDERIFVSTEAAYAMTGRLAREEGLFVGYSSGAALVAALGMAERMESG
                     AMVVMFPDGGDRYLSREV"
     gene            1333..1782
                     /locus_tag="VFK23_11650"
     CDS             1333..1782
                     /locus_tag="VFK23_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HET7319783.1"
                     /translation="MHLSPNAINSVYQHAMAAYPSECCGIVTGSGSAQRVHACKNLQD
                     ELHARDPETHPRTSREAYAMDRDEAERIMKDAAGRGERVIAFYHSHIDCGAYFSQMDK
                     EVQTVFGEPEFPDAVHVVVSVVKGNISELKGYLWDGKKQDFVPVDVS"
     gene            1938..2834
                     /locus_tag="VFK23_11655"
     CDS             1938..2834
                     /locus_tag="VFK23_11655"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET7319784.1"
                     /translation="MITILGATGHVGSKIADILIKKREPVRLVARSSDRLRPLADKQS
                     EVFAGDALDTDFLVRAFSRTDAVFVLVPPNKKSEKFMLYADAMGESIARALELAKVKY
                     VVNLSSVGADLPEGTGPIAGLHHQEARLNRIKGLNVLHLRPATFMENLLMNIDLIKTR
                     GITGNSIRGDLKFPMIATKDIAAYAAEHLVRRDFTGSLVHYLLGQRDLSLIEATEIIG
                     RKINKPGLAYVMFPYDEAEKSLVAMGLSPDVSRVYVEMSRAFNDGLIKYEERSAENTT
                     PTTFEEFCDEVFVPLFTQKKAA"
     gene            complement(2926..>3160)
                     /locus_tag="VFK23_11660"
     CDS             complement(2926..>3160)
                     /locus_tag="VFK23_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319785.1"
                     /translation="VYPWIEPFYTTGDMDQVTLPELTGMNHWVVVAVLYVLAGVMFYL
                     MEWYERRRARPFAGNATGRTILTSRSAASHGSL"
BASE COUNT          736 a          903 c          900 g          621 t
ORIGIN      
        1 gaccccgagg aggacaaaaa acgctaccat gtgactccat cgggcattgt ggtgatcgca
       61 aaagggacgg aattgcccga agaagccatg gcggctgccg cagcgcagga gaatgcctga
      121 cgcgctgtaa cagcaccaac aaggcggatg gcatgccatc cgcttttttt atgcaccctt
      181 cgctctcgaa aattcatggc gatttccgaa gtgagtgaag cgagcgaaga cgataaagat
      241 tctcacccca agaatcgagc tcccgacccc cagtgaagct caatttcaga gcgcacggcg
      301 agagagtagg cactgtttca agccgtaaga atcggagccc ccgcaatcgc tccgggggct
      361 tcaatttatc cttcactcct gatataatgc cgttcaccta tgggacaatc atctaatgat
      421 acatcccctt cccccgcagc atcggtgctc aatttgatcg gcgggacgcc cctggtccgg
      481 atctccaggg tcacgaagca tttgaacccg caggtggagg tcttcgccaa gctcgagcgc
      541 ggtaaccccg gcggctcggt gaaggaccgg gcggcctacc gtatgatcgc ggacgctgaa
      601 gaagccgggc ttcttacccg tgacaagatc attctcgatt ccacgtcggg caacacggga
      661 atcgcctatg ccatgatcgg ggccttcaaa ggttaccgcg tccgtatcgt cattcccgag
      721 aacgcgagcg tcgagagaaa aaaaatcatc tccggctttg gcgccgaatt gatttactcc
      781 agccctttcg atggcgccga cggcgcgatc ctgcttgccc gggagctcat ccgcacacac
      841 cctgatctgt attatcagcc cgatcagtac aataatccct cgaacgggaa ggcgcacgaa
      901 ctcacgacag gccccgaaat catcaggcaa tccgggggaa gagtgaccca cttcgttgcc
      961 tgtgtcggca cgggaggcac gattatgggt acgggcaggg cgctcaggaa atacaatcct
     1021 tcaatccgta ttgtatccgt ccatcccgac gacgccatgc acggtattga gggcacaaag
     1081 tatcttggtg aaggcgcgat cgtccccggc atcttcgaca cggactttcc cgatgaacgc
     1141 attttcgtaa gcaccgaggc tgcttacgca atgaccggca ggctcgcgcg cgaggaaggg
     1201 ctgttcgtgg gatactcctc gggtgcagcc ctggtagctg cgctcgggat ggccgaacgc
     1261 atggagagcg gggccatggt ggttatgttt cccgacggcg gcgaccgtta tctgagcagg
     1321 gaggtttaag ccatgcactt gtcaccgaat gccatcaact ccgtatatca acatgctatg
     1381 gcagcctacc cgtcggagtg ctgcggcatt gtgacgggct cgggcagcgc gcagcgggtc
     1441 catgcgtgca aaaaccttca ggatgagctc cacgcacggg accctgagac ccatccgcgc
     1501 accagccggg aggcatacgc catggaccgc gacgaagcgg agcggatcat gaaagacgcg
     1561 gccggccgcg gtgaaagagt catcgctttc tatcactccc atatcgactg cggggcctat
     1621 ttctcccaga tggacaaaga ggtccagacc gtattcggag aacctgaatt tcccgatgcc
     1681 gtgcatgtcg tcgtctcagt ggtgaagggg aatatcagtg aactcaaggg ctatctctgg
     1741 gacgggaaga agcaggattt cgtaccggtc gatgtctcct gacggttgcg gcataaatcg
     1801 tccaaccatc gggttccgcc ctcttcttct ttcgtccgcg ggttgacgca cacggtatcc
     1861 ggtgctacct tcaaataaaa gaaaaaggat ttgatttgcc cctgcaacct aagtcacagg
     1921 gacaaggagg tagcattatg ataaccattc tgggagcaac agggcatgtc ggcagcaaaa
     1981 tcgccgacat ccttatcaag aagagggagc cggtgcggct cgtagcgagg tcgtctgacc
     2041 gtctgaggcc cctggcggac aaacagtcgg aagtctttgc cggcgatgcg ctggacaccg
     2101 actttctggt cagggcgttc tcgcgcaccg atgccgtttt cgtcctggtc ccgccgaaca
     2161 aaaagtccga aaagttcatg ctctatgccg atgcgatggg cgaaagcatc gcccgcgcac
     2221 tggagctcgc caaggtgaag tatgttgtga acctttcgag cgtcggagcc gacctgccgg
     2281 aaggaacggg ccctattgcc ggtctgcatc accaggaagc gcgcctgaac aggatcaagg
     2341 ggctgaacgt cctgcacctg cggccggcga ccttcatgga gaatctgctc atgaatatcg
     2401 acctgatcaa gacccgcggc atcaccggca actccatccg cggcgatttg aagttcccca
     2461 tgatcgcgac caaggacatc gcggcctacg cagcggagca cctcgtaagg cgcgacttta
     2521 ccggttccct ggtccactat ctgctcggcc agcgcgattt gagcttgatc gaagcaacgg
     2581 agatcatcgg caggaagatc aacaaacccg gcctcgctta cgtgatgttc ccctatgacg
     2641 aagccgagaa gagcctggtt gccatgggac tgtcgccgga cgtgagcagg gtctatgtcg
     2701 agatgagcag ggccttcaac gacgggctca tcaaatacga agagcgctct gccgagaaca
     2761 ctacgccgac gaccttcgag gagttctgcg acgaggtgtt cgtgccgctg ttcacgcaga
     2821 agaaagcagc gtaataagcg cacgactgaa tgaataaggg gaatgtgcgg agtcgcacat
     2881 tccccgaaaa tgagatttga tgttgttatt tctgaaaagg tatatctaga gactgccgtg
     2941 cgacgccgcg gaacgggaag tgagaatggt ccgtcccgtg gcatttccgg caaacggacg
     3001 tgccctcctt ctttcatacc attccatgag ataaaacata acacccgcaa gcacatacaa
     3061 gacggcgacc acaacccagt gattcatgcc ggtcaactcc ggaagcgtta cctggtccat
     3121 gtcgcctgtc gtatagaacg gctcgatcca gggatagacg
//
LOCUS       DASSLD010000646         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1319086, whole genome shotgun sequence.
ACCESSION   DASSLD010000646 DASSLD010000000
VERSION     DASSLD010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319086"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1559
                     /locus_tag="VFK23_11665"
     CDS             <1..1559
                     /locus_tag="VFK23_11665"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET7319786.1"
                     /translation="NMAQNSGRKEQDEGGVPRHARLANIGIAVAVAVLTAFVLQGTGY
                     PLSAYFGLAALLLVFFYLFSRDIRRYRPALLGDTKKILLLALLLFATVALSQLTVRFS
                     THLAQEFHLDGKTVAFALPVSAGAMLACLLLDFRLALGLSFIASILLGILFRGDPFIP
                     IYYFLGSIVAALSVIRCKKRTAVIKAGALTGLVNIVVIACIDLYGGDLAARAAADLGA
                     GFLGAMAVTAIVSVTLPFFEAVFDIATDIKLLELLDPNHPLLKDLVYKSPGTYHHSIL
                     IGNLAEAAAEAVNENSILARVGAYYHDIGKIHKPEYYIENQHPSENKHDRLIPSMSSL
                     IIASHVKDGVEDARAHRLPKAVIDIIQQHHGTSLITYFYRKAKELNPYASIAEADYRY
                     PGPRPRTKVAAIVMLADSVEAASRALEDPTHQRIQALTDSVITRIFLDGQLSLCDLTL
                     KDLREITRSFNLVLNGIYHHRIDYPGMEFPGDKKRGDHQDKKQSEGKKPGTGKVQDAA
                     REAVAEFRSS"
     gene            1459..1926
                     /gene="ybeY"
                     /locus_tag="VFK23_11670"
     CDS             1459..1926
                     /gene="ybeY"
                     /locus_tag="VFK23_11670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET7319787.1"
                     /translation="MIIRIRNNQRGKSLALARFRTRLAKLLRSSGLPDSELSVLFTGD
                     RTMRSLNRTWRNIDRTTDVLSFSQREGRFSGIQPELLGDIVISVPQAYRQAREAGHTL
                     TREIELLLVHGLLHLLGYDHELGPTEAIKMRRREREFLTMIGASDSKRALGRR"
BASE COUNT          443 a          675 c          567 g          430 t
ORIGIN      
        1 ggaacatggc gcaaaactcc ggacgcaaag agcaggatga gggtggtgtc ccgcgccacg
       61 ccaggctcgc aaatatcggc attgccgtcg ccgtcgcagt tctcacggca ttcgtccttc
      121 aaggaaccgg atacccgctc agcgcctatt tcggtcttgc agccctcctg ctggtctttt
      181 tttatctctt ttcccgtgat ataagacggt atcgtcctgc gctcctgggg gatacgaaaa
      241 agatcctcct gctcgcgctc ctgctgtttg ctaccgttgc cctgtcgcaa cttaccgtac
      301 ggttctcgac gcacctggct caagagttcc acctggacgg gaaaaccgtc gcgtttgccc
      361 ttccggtgtc cgccggtgca atgctggcat gcctgcttct cgattttcgc ctggccctgg
      421 gattatcctt catcgccagc atcctcctcg gcattctctt ccggggcgat ccgttcatcc
      481 cgatttatta ttttctcggc agcattgtcg cggcactcag cgtgatccgg tgcaagaagc
      541 gaacagccgt gatcaaagcg ggcgccctga ccggtctcgt caacatcgtt gtcatcgcct
      601 gcatcgacct gtacgggggg gatctcgcgg cgcgcgcggc ggccgacctc ggggcgggtt
      661 tcctgggggc catggcggtc accgcgatcg tttcggtaac gctgcccttc ttcgaagcgg
      721 tcttcgacat tgccacggac atcaagctcc tggaactcct ggaccccaat catccgctgc
      781 tcaaagacct ggtctataag agccccggga cgtaccatca cagcatcctc atcgggaacc
      841 ttgcagaggc cgccgcggaa gctgtcaacg aaaactcgat ccttgcccgc gtcggggcct
      901 actatcacga catcgggaaa atccacaagc ccgaatacta catcgaaaac cagcacccct
      961 ccgagaacaa gcatgaccgg ctcattcctt ccatgagcag cctgatcatc gcgtcgcacg
     1021 tcaaggacgg cgtggaggat gcgcgtgcgc accgcctgcc gaaagcggtc atcgacatca
     1081 tccagcagca ccacggcacg tctttgatca cgtactttta ccggaaggcg aaagaactga
     1141 acccctacgc ttcgatcgcc gaggcagatt accgctaccc gggaccgcgg ccccgcacga
     1201 aggttgccgc catcgtgatg ctggcggact ccgtcgaggc tgcctcgcgt gcgctggaag
     1261 atcccacgca ccagcgcatc caggcgctca cggacagtgt catcacgcgt atcttcctgg
     1321 acggccagct ctccctgtgc gacctcaccc tcaaggacct gcgggaaatc acccggagtt
     1381 tcaacctcgt cctcaacggc atataccacc accgtatcga ctatcccggc atggagttcc
     1441 ctggagacaa aaaacgcggt gatcatcagg ataagaaaca atcagagggg aaaaagcctg
     1501 gcactggcaa ggttcaggac gcggctcgcg aagctgttgc ggagttccgg tcttcctgac
     1561 tcggaactga gcgtcctctt caccggcgac cggaccatgc gctccctcaa ccgcacctgg
     1621 cgcaacatcg accggaccac ggacgtgctg tcgttctctc agcgcgaggg aaggttctcc
     1681 ggcatccagc ccgaacttct cggtgacata gtcatctcgg tgccgcaggc gtatcggcag
     1741 gcccgggaag cgggtcacac gctgacgcgc gaaatcgaac tcctgctcgt ccacgggctg
     1801 ctccatcttc tgggatatga ccacgagctc ggcccgacgg aagccatcaa aatgcggcgc
     1861 cgggaacggg aattccttac aatgatcggc gcatctgact ctaaaagagc tttgggacgc
     1921 agataaaatc ggatagccaa aagtaggcgc ttctaagcga cgcggataag gctgagatca
     1981 ctgcagaggg agaaacattc accctgaaaa actctttttg tctcaaggct caggttatct
     2041 gcaaaaatct gcgttcatcc gcgtccaact gccgttttca cacgggtgat tcgtttgaaa
     2101 ccgaaaacat ggttc
//
LOCUS       DASSLD010000647         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_799010,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000647 DASSLD010000000
VERSION     DASSLD010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799010"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..792)
                     /locus_tag="VFK23_11675"
     CDS             complement(256..792)
                     /locus_tag="VFK23_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7319788.1"
                     /translation="MNKTPYITNIVAERTIMKSNHIAAQIKCSIKAYSARDTEAAVIR
                     RLRQRQLKRPRPGGKRILVAIDDRFLRTLMTAVLYQAGYTVIEAIGGADAVRKFWNSP
                     EAIDLLLLDMDMPKNSCKEVYDMIRQAKPDIKVVFAGSYPAEIMRKRGLFGTDTHFLS
                     KPFGPRVLLNKISEVLDG"
     gene            complement(1110..1667)
                     /locus_tag="VFK23_11680"
     CDS             complement(1110..1667)
                     /locus_tag="VFK23_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET7319789.1"
                     /translation="MSLVLLVSSCAAPPARIEPGALKKRPQPKIVTAALEKQIHSLIN
                     KERRKQGLSSLEWDDALSGIAQKHSRDMAARNYFDHYSPEGHDFSYRYQQGGYQCVVR
                     TGRTVYMGAENIALNHIYDSVTTVNGRAYYDWNTQDKIAETTVRGWMKSRGHRKNILT
                     PYFKHEGIGVIIAPDDRVYITQNFC"
     gene            complement(1757..>2161)
                     /locus_tag="VFK23_11685"
     CDS             complement(1757..>2161)
                     /locus_tag="VFK23_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7319790.1"
                     /translation="RRAVLYVADFLGGFPGFTVTLISIIPEPEEDFFESDAAMNTWIK
                     DKVDATNKMLANYKQVLMQSGFLEEKVRFRSCVGEAKSFSDAILETRCDLTCCTVVVG
                     RHHKTKTEEFLFGSTSNRLIHEAKNCAVWVVE"
BASE COUNT          495 a          549 c          568 g          549 t
ORIGIN      
        1 tcggctcccg ctcgtgaccc agggcggcgg gaaatggtcc ctcgaccggg tactcgtaaa
       61 gcaggataat taacgcgcct taccaagcca ggtgggcgta caacatcgga acacgccgac
      121 gggcgtttcc atcaatgggc cgttagattc taccggcccg tttttttctg cgtcggcggg
      181 atgtgacaat tcgggatctc tataaaagcg gaactctttc gccccttttt ttaaaactga
      241 aaaatcttta acgcttcacc catcaagaac ttcgctgatt ttgttgagaa gaacccgagg
      301 gccaaacggc tttgaaagaa aatgggtatc ggtaccgaac aatccgcgtt ttcgcattat
      361 ttctgcagga taactgcctg caaataccac tttgatatcc ggcttcgctt gcctgatcat
      421 gtcgtaaact tccttgcagc tattcttcgg catatccatg tccaacagca ggagatctat
      481 agcctcggga gaattccaaa atttacgaac agcgtctgcg ccgccgattg cctcgatgac
      541 agtgtaccct gcctggtaaa gcacggccgt catcagagtt cgaagaaatc tgtcgtcaat
      601 tgccacgagg atccttttgc cgcccggtcg gggccgcttg agctgcctct gccgcaaacg
      661 tctgatgact gcggcttccg tgtcgcgtgc gctgtacgcc tttatggaac acttgatctg
      721 tgcggcgata tgattacttt tcataatggt cctttctgcg acaatgttcg tgatgtatgg
      781 ggtcttgttc attgcttgag accagcttct tcagcataaa gtcaaattgc ctaactaata
      841 ataaagcaaa tgacgtacca tctgagggag gatccaataa tctcttattt ttatgatact
      901 tagtacgtat gagtccaggt atcactccgc tttgtcccct gggcctgtat agtttcacat
      961 acagtagaag atgaatgagg ggcaaatcaa aggctgtttg ctgcgggaaa tcaagggatt
     1021 aaggcttggt ggaaaatcca gacagtgtgt gaaacttaac gaaggcagta gtgatggaca
     1081 tgctggtgcg aaaagtaaat gacggaccat cagcagaagt tctgcgtaat ataaaccctg
     1141 tcatcgggag cgatgatgac accgattcct tcatgcttga aatagggggt gagaatgttc
     1201 ttccggtggc cccggctctt catccatccc cgtacggtcg tttctgcgat cttgtcctgg
     1261 gtattccagt cataataagc cctgccattt accgtggtga ccgaatcata gatgtgattg
     1321 agcgcgatat tctcggcgcc catatacacg gttctgcctg tccggacaac gcactgatat
     1381 cctccctgct gataccggta tgaaaaatcg tgaccttcgg gagaatagtg atcgaaatag
     1441 ttccttgctg ccatgtcccg gctgtgtttt tgcgcaatgc cggaaagcgc gtcatcccat
     1501 tcaagcgaag acaggccctg tttccgccgt tctttattga tgagggagtg gatctgtttt
     1561 tccagagccg ccgtgacgat cttcggttgc ggccgctttt tgagggcacc aggttcgatc
     1621 cgtgcagggg gggcggcgca ggatgataca aggagaacga gtgacaccag gaatccggtt
     1681 attgtagtgc ccctattgat catcgtgagc attgaacgaa cttgcctttc aaagatcggt
     1741 gaagatacga agaccctcac tccaccaccc acaccgcgca gttcttcgcc tcgtggatga
     1801 gcctgtttga cgtgctgccg aagaggaact cctcggtctt cgttttgtgg tggcggccga
     1861 cgacgacggt gcagcacgta aggtcgcagc gggtctcgag gatcgcgtcg gaaaaggact
     1921 ttgcttctcc cacgcaggaa cggaagcgca ccttctcttc gaggaacccg gactgcatca
     1981 ggacctgctt gtaattcgcc agcatcttgt tggtagcatc gactttatcc ttgatccatg
     2041 tgttcattgc ggcgtcgctc tcgaagaagt cctcctcggg ttcggggatg atcgagatga
     2101 gggtgaccgt gaaccccggg aaccccccga ggaaatcggc gacatacaaa acggcgcggc
     2161 g
//
LOCUS       DASSLD010000648         6917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_112598,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000648 DASSLD010000000
VERSION     DASSLD010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112598"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VFK23_11690"
     CDS             <1..565
                     /locus_tag="VFK23_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319791.1"
                     /translation="LKELSSLFQEVVLVDIVFLPEVRSIIKQFGNVKLLRHDVTNMAK
                     HLHENIRRGLRELPDAAPAAPEIDEKAGLVVSLNLLSQLWVVPRSYARIKLSGLNEEQ
                     VDDWCGRIVQSHYTFLRSIPCDVCLVADHEFVKRDQKGRIVSQGSTVSGLFLPEPDAS
                     WTWNIIPMGENRQYLSKELNVGAWHLQ"
     gene            603..1091
                     /locus_tag="VFK23_11695"
     CDS             603..1091
                     /locus_tag="VFK23_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008242630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7319792.1"
                     /translation="MFVEVSTEYQIEIVESLAREIWTEHYIPIIGREQVDYMLATFQS
                     RRAIGEQIRTGIAYFLIKEDDAHIGYMAVQPKGRELFLSKIYVKSSRRGLGYGRKAVQ
                     YAEKVARDLGLDKIVLTVNKNNVNSIKAYEKIGFTNAGPVVQDIGSGFVMDDYKMEKK
                     LS"
     gene            1261..1488
                     /locus_tag="VFK23_11700"
     CDS             1261..1488
                     /locus_tag="VFK23_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319793.1"
                     /translation="MVKINQEELKQKIENLKKKAAEKASKAGGKKSDPDARQARKKVK
                     RAQRKLRTAKTYKQAGKKKAEAAAGGEKAPA"
     gene            1503..2279
                     /locus_tag="VFK23_11705"
     CDS             1503..2279
                     /locus_tag="VFK23_11705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET7319794.1"
                     /translation="MSRFFSHGKRVLAGVCAFLALAGLAACAGIVKEPATAPAPDTEH
                     RALSSIPSTTAKGPTPAIAPVQPASPGPQSLPVREPGYRETGIAGWYGKEFQGKKTAS
                     GKVFDMEGLSAAHRTLPLGTVVRVTNLDNLKNIDVKIIDRGPFVKNRILDLSYGAARE
                     LGFLENGTARVKVETLKPVRGAARYTVEAAAFTEEENARLLKERLSKKFELVYIVQTE
                     TSIARLYHVRIGSYTAEERAKQIAGKLMLEGLEPVVLLKD"
     gene            2314..3813
                     /locus_tag="VFK23_11710"
     CDS             2314..3813
                     /locus_tag="VFK23_11710"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET7319795.1"
                     /translation="MKINVGTGKIEKLKTDGAVLLLFDGEKMGKAAARLDESLGGIIG
                     RVIKRGDFKARPGAVHLLYPGSAAAPERVLLAGLGKRGEFTPNRLRQAVGKAATALRA
                     AGATDAAVLVDGIGMDPVETGQSIAEGALLGLYRFLRYKTDEENERKKDIRTVTLVSE
                     SAAAVNALKKGVRTGEAIADSVMMVRDMVSSPPVDMTPAAIAAKSREISRLSGLRIQV
                     LERRQMEKLGMGGLLGVASGSMQPPKFIIVEYRKGGKKPFIALVGKTITFDSGGISIK
                     PAENMDRMKDDMSGGAAVLGAIRTAAALGLPLNVVGLLPATENMPSGSAYKPGDVLRT
                     MSGQTIEILNTDAEGRLILSDGLAYACRYKPAVIVDIATLTGACGIALGQEATGMLGT
                     HEGFKRKMREAGEKTGERVWELPLWEEYYELIKSDIADMKNTGGRQGGVITAAALLSK
                     FVQKYPWVHLDIAATAWTDKDRPYTPKGATGVGVRLLTQFLRDYAAETR"
     gene            3971..4597
                     /locus_tag="VFK23_11715"
     CDS             3971..4597
                     /locus_tag="VFK23_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319796.1"
                     /translation="MPVDLTYTEAKANTEKLLIKGRFTKADVFITRSGQDRFVVKDYA
                     QKGFWERNLVGRIVIGREARAYAALAGTEGLPSRFKRLSAFSFAVEYLEGKDFGDVEP
                     GEIGAAVIGQFERIVRGMHGRGWVHLDLHRRTNILLVGGRIYIVDLASALHPGGVPFI
                     GKFLTRLIGIADTLSLIKMKTIYGPELMSPRERKWLRIRNKVMPSKWE"
     gene            complement(4624..5346)
                     /locus_tag="VFK23_11720"
     CDS             complement(4624..5346)
                     /locus_tag="VFK23_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319797.1"
                     /translation="MKKRFILVIALFVIALAAPASIRASAPASKCEDTDRDCKEFEQM
                     LDAQQPDKVIARYEAAKTAQYSAGALRYIGDAYLLLASREDITPEQELGYYQKALEVK
                     HYIAYMGLYFFYAQKDEEKALGFLREYVKTGPKDTVPYVILGESELNKKHYKLADAYL
                     REGKKVAHAHSPRVDWLLFQANYLLGNYQFAAEMLESALTNGTFEKEVTALSSDVRFK
                     GIESRPEFQKHRSLFKAASGRP"
     gene            5657..6685
                     /locus_tag="VFK23_11725"
     CDS             5657..6685
                     /locus_tag="VFK23_11725"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET7319798.1"
                     /translation="MNRRESIRQVMKGERSSRLPRALFGGGRWAYRQAGLSMSGLEND
                     PHGFAVKLAELYAGLDTDIVFAGSGLNTFPAEAIGGELAFKEEQAPLLAHPIIRKIED
                     ARYFEQVDLSGSPRTLALVEMIAHLRQLLPDRFLCCTSWGPFTWGMVLCDWTLLQDRI
                     VSEPVFIREVCELGVRLSSALFRKLIDRGLVDGIVIADGAATLVPLDLYHDTILPCEK
                     KLFHEFAGQGVARFLHQCGAIAPQLGLYPETGADCITLDSGVELGQAYALYSPEIVTA
                     GNVDVVKTVFGGNPGQICEAVFASLAGIADPFRNFILMPSCDLPPETSLGNARAFLAC
                     ADQVSNHV"
BASE COUNT         1516 a         1916 c         2102 g         1383 t
ORIGIN      
        1 gctcaaggag ctgtcgtctc tgtttcagga ggtggtcctc gttgatattg tcttcctgcc
       61 ggaagtccgg agcatcatta aacaattcgg caatgttaag ctccttcggc atgacgtaac
      121 gaacatggca aagcatcttc atgaaaacat ccggcgcggt ctccgcgagc tgccggatgc
      181 cgcgcccgcg gcgccggaaa ttgatgaaaa agcgggtctg gtcgtgtccc tgaatctcct
      241 ctcccagctc tgggtcgtcc cgcggtctta cgcacgcata aaactttccg gcctcaatga
      301 ggaacaggtc gatgactggt gcgggcgtat tgtgcaatcc cattatactt ttctgcgttc
      361 aataccctgc gatgtctgtc tcgtcgcaga tcatgaattc gtaaaacgcg accaaaaggg
      421 gcgcatcgtc agtcaaggat cgacggtatc cggcctcttt ctgcctgagc ccgatgcctc
      481 ttggacctgg aatatcatcc cgatgggtga aaaccgtcag tatctgtcca aagagctgaa
      541 cgtcggcgcg tggcacttgc aataacagcc gggaagaccg acaaagccag acaggggaaa
      601 ccatgtttgt cgaagtctcg accgaatatc agatagaaat cgtcgaatct ctggccaggg
      661 agatctggac cgagcattac atccctatta tcggaaggga acaggtggat tacatgctcg
      721 ccacgtttca gtccaggcgg gccatcgggg agcagatacg gaccgggatc gcctattttc
      781 tgatcaagga agacgacgca cacatcgggt atatggcagt ccaaccgaag ggcagggaat
      841 tgttcttaag caagatatac gtgaagtctt cgaggcgtgg tttggggtac ggaagaaagg
      901 ctgttcaata cgcggaaaaa gtggccaggg atctgggttt ggacaaaatc gttctgacgg
      961 tgaacaagaa caatgtaaat tccatcaagg cgtacgagaa gatcgggttt acgaacgcag
     1021 gccccgtcgt ccaggacatc ggcagcggct ttgtcatgga cgattataaa atggagaaaa
     1081 agctctcgta agtcataggt ttctttcccg aggtcgtcat tcgggcccct cgcagtcatt
     1141 tcaagctttg tcttcgttcc ccgtggcaac atgacacgaa atctcttgat ttcgcatgta
     1201 cttttgtcta ttattgtgct tcgcatagtt gtgttcttat cttgaaggag gatggcaaac
     1261 gtggtgaaga tcaatcagga agagctcaag caaaagatcg aaaatctgaa aaagaaggcg
     1321 gcagagaaag cttcgaaggc cggcggtaag aagtccgacc ctgacgcacg ccaggcccgc
     1381 aagaaagtca aacgggccca gcggaagctg cgcacggcta agacatacaa gcaggcgggc
     1441 aagaaaaagg cggaagccgc ggcgggcggt gaaaaggcgc cggcttaaca gaaggaaacg
     1501 cggtgtcacg ttttttttct cacggaaaga gggtactggc cggtgtctgc gcatttcttg
     1561 cgctcgcggg actggcggcg tgcgcaggca tcgtgaagga acctgcgacc gcgccggcgc
     1621 ctgacaccga gcatcgggcg ctcagcagta tcccctcgac aacagcgaag gggccaacgc
     1681 cggcgattgc tcccgtgcag ccggcctccc ccggacctca gtccctccct gttcgggaac
     1741 cgggctaccg ggaaacgggc attgcgggct ggtacgggaa ggaatttcag gggaaaaaga
     1801 ccgcttccgg caaggttttc gacatggaag gcctctcggc cgcccaccgg acgctgccgc
     1861 tcggcacggt cgtccgagtt acgaacctcg acaatttaaa aaacatcgat gtaaagatca
     1921 tcgaccgggg gccgttcgta aaaaaccgga tcctggacct gtcgtacgga gcagccaggg
     1981 aactgggatt cctggaaaac ggcactgccc gggtgaaggt agagactttg aaaccggtcc
     2041 gcggcgctgc gcgctacaca gtcgaggccg cggcgtttac ggaagaggaa aacgcgcgtc
     2101 tgctcaagga acgtctttcg aagaagttcg agctggtcta tatcgtgcag acagaaacct
     2161 caatcgcccg tttataccat gtgcgaatcg gctcctacac agcggaggag cgggcgaagc
     2221 agatcgcggg caaactgatg ctcgaaggct tggaaccggt cgtcttactc aaagattaac
     2281 aggcttacca tgataacatc cgggtgagga atcatgaaga tcaacgttgg gacgggcaag
     2341 atagaaaaac tgaagacaga cggcgcggtg ctgcttcttt ttgacggaga aaaaatgggg
     2401 aaggccgcgg cccggctgga cgagtcgctc ggcggcatca taggacgcgt cataaagcgc
     2461 ggagacttca aggccaggcc cggcgcggtc caccttctgt atccgggcag tgccgccgcg
     2521 ccggagcggg tgctccttgc cggcctgggt aagcgcggag aatttacacc caaccggctg
     2581 cgtcaggctg tgggcaaggc ggcgacggca ctcagggctg cgggcgcaac cgatgcggcg
     2641 gtcctggtcg acggcatcgg catggatccg gtggagaccg gtcagtccat tgcagagggg
     2701 gcccttctcg ggctctaccg ctttctcagg tacaagacag acgaggagaa cgaacgcaag
     2761 aaggacatcc gtacagtcac cctggtaagt gaatcggcgg ccgcggtcaa tgccctgaag
     2821 aagggtgtaa gaaccggcga ggcgatcgcc gattccgtta tgatggtccg cgacatggta
     2881 agctcacctc ccgttgacat gacgccggcg gcaatcgcag cgaagtcgag ggagatctcg
     2941 cgcctgtcgg gcctcaggat ccaggtcctt gagcgaaggc agatggaaaa gctcggcatg
     3001 ggcggactcc tgggcgtcgc gtcgggcagc atgcagccgc cgaagttcat catagtcgaa
     3061 taccggaagg gcggcaaaaa gcccttcatc gcactcgtgg gcaagaccat tacctttgat
     3121 tccggcggca tctcaatcaa gcccgcggaa aacatggaca ggatgaagga cgacatgtcc
     3181 ggcggagccg cggtcctggg cgcgatccgc acggccgcgg cgctgggact cccgctcaat
     3241 gtcgtgggac tgctccccgc cacggagaac atgccgagcg gcagcgcgta caagcccggc
     3301 gacgtccttc gcaccatgtc cggccagacc atcgagatcc tcaacaccga cgcagagggg
     3361 cgtctcatcc tgtccgatgg gctcgcctac gcctgccgct acaagccggc ggtgatcgtg
     3421 gatatcgcga ccctgactgg tgcatgcggc atcgcgctcg gccaggaggc gacgggcatg
     3481 ctcggcacgc atgaaggttt caagcggaag atgcgagagg ccggcgagaa gaccggcgag
     3541 cgcgtgtggg agcttccgct ctgggaggag tactacgagc tgatcaagag cgacatcgcg
     3601 gacatgaaaa acaccggcgg cagacaggga ggcgtgatca cggccgcggc gctcctctcg
     3661 aagttcgtgc agaaataccc ctgggtgcac ctcgacatcg ccgcgactgc ctggaccgat
     3721 aaggaccggc cctacacccc gaagggcgca accggcgtcg gcgtaaggct cctgacgcag
     3781 ttcctgcgtg attacgccgc ggaaacgagg taatgcagcc gaaagatgag ggacgaggga
     3841 agagagattc aaatactttg tcgtccctcg tccaagcgag ccagtgccca ttgcttacga
     3901 gtgccttgcg cgagtgcagg gcacacagcg agcgatcgcc catcgtccct cgatgctatc
     3961 atccccatac atgccggttg acctaacata caccgaagca aaagcaaata ccgagaagct
     4021 cctgatcaag ggacggttca ccaaggccga tgtgttcatc acgcggtcag gacaagaccg
     4081 cttcgtggtg aaggactatg cgcagaaggg attctgggag cgaaacctcg tcggacgcat
     4141 cgtcatcgga cgcgaggccc gcgcctatgc cgcgcttgcc ggaacggagg gattgccctc
     4201 acggttcaaa cgcttatcgg ccttttcgtt tgccgtcgag tatctggaag ggaaagattt
     4261 cggcgacgtt gagccgggag agatcggggc cgcagtgata ggtcagttcg agcggatcgt
     4321 gcggggcatg cacgggcgcg gatgggtcca tctcgacctt caccgccgta cgaacatcct
     4381 gctcgtcggc ggcaggatct atatcgtgga cctggcgtcg gcccttcatc cgggcggcgt
     4441 cccttttatc ggaaagttct tgaccagact gatcggcata gccgacaccc tgtcgctcat
     4501 aaaaatgaaa accatttacg ggcccgaact catgtctccg cgggagcgaa aatggctccg
     4561 gatcaggaac aaggtcatgc cgtcgaagtg ggaatgatcg ggtaaggagg gggacttcag
     4621 aggttacgga cgaccggatg ctgctttgaa caggctgcgg tgtttctgaa attcaggacg
     4681 ggactcgatg cccttgaacc ggacgtccga tgacaacgcc gtcacttctt tctcgaaagt
     4741 tccattcgtt agcgcacttt cgagcatctc cgctgcaaat tggtaatttc ccagcaggta
     4801 gtttgcctga aagagcagcc agtccacgcg cggggaatgc gcgtgcgcaa cctttttccc
     4861 ttcccgcaga taggcgtccg caagcttgta gtgtttttta ttcagctcgg attccccgag
     4921 gatgacatac ggcaccgtgt ccttcggtcc ggtcttgaca tattcccgga ggaagccgag
     4981 agccttttcc tcgtccttct gagcgtagaa gaaatacagg cccatgtacg cgatgtagtg
     5041 ctttacctcg agcgccttct gatagtaacc cagttcctgt tccggcgtga tgtcctcacg
     5101 ggaggcgagc agcaaatagg catcgccgat gtagcggagg gcgcctgcgg agtattgagc
     5161 cgtctttgcc gcttcgtacc gggctatgac cttatcgggc tgctgcgcgt cgagcatctg
     5221 ttcgaactct ttgcagtccc tgtccgtatc ttcgcatttg gatgcgggag ccgatgcccg
     5281 aattgacgca ggggctgcga gggcgatgac gaaaagggcg atgacgagta tgaagcgctt
     5341 cttcatccgg tctccttcga cgtatggtcg cgctactata aaacaggggc agacaatccg
     5401 aagcaagttc ggatgataca gtatactatc gagcgtttgg tgtcaacagc gttttctgtt
     5461 tcgaaacccc aatcagggcg gctccccccc gcttatttcc cgggttgact gcggcgcagt
     5521 ctctccattg acgcctcaaa tctttctggg gaactattaa cggttggcag ctgaataatg
     5581 gcgaatcaag aggcatgcga ggtagagaag gaaaaatgaa gaaagagcac ggcggtctca
     5641 tgtcctcgaa attatcatga ataggcggga atcgatcaga caggttatga aaggcgagcg
     5701 cagttcgcgg ctaccgcggg ctctgttcgg cggcgggcga tgggcgtacc gtcaggccgg
     5761 gctcagcatg tccgggctgg aaaacgatcc gcacggcttc gccgtgaagc tcgccgaatt
     5821 atacgccggc ctcgataccg acatcgtttt cgccggatcg ggcctgaaca cttttcccgc
     5881 cgaggccatc ggcggagagc tcgcctttaa ggaggagcag gccccgctgc tcgctcatcc
     5941 catcatccgg aagatcgaag atgcgcgtta cttcgagcag gtggaccttt ccggatctcc
     6001 ccgaaccctc gcgctggtgg agatgatcgc gcacctgcgg cagctccttc ccgaccgctt
     6061 tctctgctgc acctcctggg ggccgttcac ctggggcatg gtgctctgcg actggaccct
     6121 gctgcaggac aggatcgtgt ccgaacccgt gttcatccgg gaggtatgcg agctcggtgt
     6181 gcgtctttcg tcggccctgt tccggaaact cattgatcgc ggcctggtgg acggcatcgt
     6241 catcgcggac ggcgcagcca cgctggtccc gctcgatctc tatcacgata cgatacttcc
     6301 gtgcgaaaag aaactctttc atgagttcgc ggggcagggc gtggcgaggt ttctccatca
     6361 gtgcggcgcc atcgcacccc agctcggcct ctacccggag accggagcgg actgcattac
     6421 cctggattcc ggtgttgaac tcggtcaggc ctatgcgctc tattcgccgg agatcgtcac
     6481 cgccgggaat gtggatgtgg tcaagactgt ttttggcgga aatccgggtc agatctgcga
     6541 ggctgttttc gcctccctgg ccgggattgc cgatccgttc cggaatttta tcctgatgcc
     6601 gtcctgcgac ctgccgccgg aaacatcgct cgggaatgcc cgggcattcc ttgcctgtgc
     6661 ggatcaggtt tcgaatcatg tgtaatgacg ggcctttgct gctcacggcg aggatcaggt
     6721 tcattgacac gtccgttccg aatagagtaa tatttagtca tgccgaaatt caagatcatt
     6781 tcggaattca aaccgaccgg cgaccagccc cgggccatcg acatgctttc cgagggcatc
     6841 gtgaagggca ttcgcgacca ggtccttctg ggcgtcaccg gctcgggtaa gaccttcact
     6901 atggcgaacg tcgtgga
//
LOCUS       DASSLD010000649         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_895318,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000649 DASSLD010000000
VERSION     DASSLD010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895318"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..1419
                     /gene="sfsA"
                     /locus_tag="VFK23_11730"
     CDS             268..1419
                     /gene="sfsA"
                     /locus_tag="VFK23_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HET7319799.1"
                     /translation="MKLFGNIITTSFISRPNRFVVECRIDGRKVRAYLPNPGRLWELL
                     LPGVRLYLIEFPPSLERSLKYMAVAVERNGIPIMLHTHHNNTVARRLIEEKRIPGLEG
                     ATIIKPEHTIGHSRFDFLLRKGGRDLILEVKSCTLFNETLAMFPDAVTARGTKHLLEL
                     ASLSRKGMNAAVLFIVHAPNVRFFMPEHHTDLDFSQTLLSVKDRVMVKAVSVGWNRDL
                     TIGDEVRELTIPWDLVMQESHDSGSYIVVLRLLRDRRISVGGLGDVKFRKGFYLYTGS
                     AKKDLTRRINRHRSERKNLFWHIDYLREFAGYHAALPIRTSADLECEIATSLNEIADW
                     RINGFGSSDCTCETHLFGMKDDPLLSPEFIRRLLHFRMGRLEETLSKGL"
     gene            1464..2165
                     /locus_tag="VFK23_11735"
     CDS             1464..2165
                     /locus_tag="VFK23_11735"
                     /inference="COORDINATES: protein motif:HMM:NF025615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HET7319800.1"
                     /translation="MRPEAKGYFLKEYGSWSVLIVAYLIGIGVSRAFDWRLVPLLLAL
                     GLLINSKQAFSKWNRGTGDRRSLVIFAAQIAVAASILIAVFGSDIPMLLPLLIFPAAY
                     LLSSKIAGEHFILTELLGFALISLSAVLAKFLLTGGLDVRLFLGVALYFTAGVFKVKT
                     LLLRKTRDRVLGVLYLLLSVFVYYRLHIPLIILLPLADNLIVALNPYKVKLKTTGWIE
                     VAKSLALLGLFIAFY"
     gene            2529..>2740
                     /locus_tag="VFK23_11740"
     CDS             2529..>2740
                     /locus_tag="VFK23_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319801.1"
                     /translation="MRRTLSILSMFLLFVTAIVSPASAEKVARPITAWPFLVHEENQD
                     HAETDVFFSLYHYERKDTRTRYSFGY"
BASE COUNT          587 a          794 c          673 g          686 t
ORIGIN      
        1 gaagcgacat cttgtatggc ctacgttcat tctattatga gaccgttaat aatttaaagg
       61 taatcttctt tcgcgttcgt gtcaacctcg cttttcacaa tattgtctgt ggccacatga
      121 gatcgttgcg caaaaaacgc gcctgcccgt gcctgtcctg gcgaccagtt tctgataacg
      181 aggatttacc gcgatcatgt cacttcccat tcataacctt ccctcctccc tgatgacaac
      241 tccggccaaa tcggttacta tggctccatg aaactcttcg ggaacatcat cactacctct
      301 ttcatatccc gtcccaaccg cttcgtggtc gagtgcagga tcgatggacg gaaagtccgc
      361 gcctaccttc ccaaccccgg caggctctgg gaacttcttc ttcccggggt ccggctttat
      421 ctcatcgaat ttcccccttc gttagagaga agcctcaagt acatggcagt tgccgtggag
      481 cgaaacggca tccccatcat gctccatacc catcacaaca ataccgttgc ccgccgtctc
      541 atagaagaga agaggatccc cggtcttgaa ggcgcaacga tcataaagcc ggagcatacg
      601 atcgggcaca gcaggttcga cttccttttg cggaagggtg gcagggacct cattctcgaa
      661 gtcaagtcct gtaccctctt taacgagacc ctggccatgt tccctgacgc ggtcactgcc
      721 cggggaacga agcatctcct ggaactggcg tcactgtcga gaaagggcat gaatgcggca
      781 gttctgttca ttgtccacgc gcccaatgtc cgcttcttca tgcctgagca tcacacggac
      841 ctcgatttct cgcaaacgct cctgtcggta aaagaccgcg tcatggtgaa ggccgtatcg
      901 gtgggctgga accgcgatct caccatcgga gacgaagtgc gggaactcac aataccctgg
      961 gatctggtca tgcaggagtc ccatgacagc ggaagctaca tcgtcgtgct acggcttcta
     1021 cgggacagac ggatatctgt cggcgggctc ggcgatgtca agttcaggaa aggcttttac
     1081 ctctatacag gctctgcgaa aaaggacctg acccggcgga tcaacaggca ccggagcgaa
     1141 cgaaagaacc tcttctggca catcgattat ctgagagagt ttgccgggta tcatgccgcc
     1201 cttcccatac ggacgagcgc agacctggag tgtgagattg cgacgtcgtt gaatgaaata
     1261 gccgattggc gtatcaatgg tttcggctct tcggactgca cctgcgagac ccatctcttc
     1321 ggcatgaagg acgatccatt gctttcgccg gagttcatca ggaggcttct tcatttccgg
     1381 atgggcaggt tggaggagac gctctcgaaa ggcctatgat ttctctcata acgcaacttc
     1441 ctcattaatg ttatactctc tgcatgagac ccgaagcgaa aggttacttc ctcaaagaat
     1501 acggctcctg gagtgtcctc atcgttgctt atctcatcgg catcggggtg agcagggcct
     1561 ttgactggag actggttcct ctcctccttg ctctcgggct tttgatcaac tccaagcagg
     1621 ctttctcgaa atggaaccgc gggaccggtg acaggcgttc actcgtcatc ttcgccgccc
     1681 aaatcgccgt tgccgcctcg attctcatcg ctgttttcgg cagcgacatt cccatgctgc
     1741 tgccgctttt gatcttcccg gcggcttacc ttctttcaag caagatcgcg ggcgaacatt
     1801 ttatcctgac cgagcttttg ggcttcgccc tcatcagcct gtccgcggtg ctcgcgaaat
     1861 ttcttctcac gggcgggctc gatgtacggc tctttctcgg agtcgcgctt tacttcaccg
     1921 ccggcgtatt caaggtcaag accctgctcc tccgaaagac gcgggatcgg gtgctgggcg
     1981 tcctgtatct gctgctctcc gttttcgtct attaccggtt gcatatcccg ctcatcatcc
     2041 tcctgcctct cgccgacaac ctcatcgtcg ctctgaaccc gtacaaggtg aagctcaaga
     2101 ccaccggctg gatcgaagtg gccaaaagcc tcgcactctt ggggcttttc attgccttct
     2161 actgatcggc cgccgaaaaa acttttgcag gccggagtct gcgactacca tggagagcgg
     2221 caggatcgtc atccagggtg cgcctgcgga aataggaaac gacgagcggg tgatcgccct
     2281 tccggttgta ctatcctgcg cgttttacta ttccctccgt aatatatatc atcctgaatt
     2341 aaacgagttc tcattatttg cgtctcggtg agttccttcg cccgcgacat cccttctcac
     2401 gggtcccctc tgaaaaaaac gtgcagacaa aagtaaattt gtgttgatgc taaaaatagt
     2461 tggattccct cagtcacctg tcagacctct gttgtataag catatcagat acacaaggag
     2521 acggcagcat gagaagaacg ctctcgatac tgtctatgtt ccttttgttc gtgacggcta
     2581 ttgtgtcgcc cgcctctgcc gagaaggtag cccgacccat cacggcctgg ccatttctgg
     2641 tgcatgaaga aaatcaagac cacgccgaaa ccgatgtttt cttttccctg taccattacg
     2701 agcgcaagga cacacggacc agatattcct tcgggtatat
//
LOCUS       DASSLD010000650         7383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_928177,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000650 DASSLD010000000
VERSION     DASSLD010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928177"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VFK23_11745"
     CDS             complement(<1..472)
                     /locus_tag="VFK23_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7319802.1"
                     /translation="MPTYNGVNHLAMATRDMDETIRFWRDLLGMRLIAGLGKPGYRHY
                     FFEISAHDMIAFFEWPAVEPVVEKEHGRPAKGPFVFDHVSFGVETEDDLWALKDRINA
                     AGIWVSEVIDHGFIHSIYSYDPNGIPIEFSYAVPGTDVRRTPRMADSKPSAVAQE"
     gene            753..1085
                     /locus_tag="VFK23_11750"
     CDS             753..1085
                     /locus_tag="VFK23_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319803.1"
                     /translation="MKGRFQMENHTLRDIIEVEKEIQKSLDSTKETLNDWLDARKKEA
                     EEELARSEQELLASFQQSREQVMRDAGGRASVLATAAEERAARMTELQNDLLAGIVAG
                     HIKKILPG"
     gene            1089..2969
                     /locus_tag="VFK23_11755"
     CDS             1089..2969
                     /locus_tag="VFK23_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HET7319804.1"
                     /translation="MIVRMAKIEIVGAKELLQDVLSRLRDLGIFQIEPVAVGILENGK
                     IDDDIRSFTLDEKTMFERVFLEELRTKVGNLLSRLPKLPVRKSYLDPRPVIDTLSAIV
                     ERHTAAVAARNERSEVLRKERAELERYALFLGALAPLVGSARETPDLEFIGLTIREPD
                     MVGRLKEAISRMTDWKYELVTEAAKDGTLVGLITVEKELAGVVKKTLSDEQVLELVLP
                     PTFSGFSFSEKVSYVQKRIAEVTREIDAIEASLAQFACRWIPIYRSVREWIDDRLSLL
                     SATTAVFETRMCFFITGWMPQRDVAAVRGTLKDVYQGNIVVAEKEIREEDIDRVPIIL
                     KNPPYFRPFEIFTSLLPLPAYTSYDPTPFIGLFFPIFFGMILGDAGYGLLLALLAVFL
                     KRRYRQQKYVRDGAAILLVASLYSVFFGVLFGEFFGDLPERLFHLQPICVERRTAIVP
                     MIFFTLAVGVAHVLLGLFFGVVTGLRKKEKKEAAYKLISIVIILGIIFVLSSLSGVLP
                     SVLARPIIIAILFLTPLLFFTGGILAPLELLKSIGNIISYVRIMAIGLTSVLLAFVAN
                     NLGGLTGDVVTGIVVAGLIHFLNIILGVFSPTIHSLRLHYVEFFSKFMEHGGRRFEPL
                     KR"
     gene            3085..3303
                     /locus_tag="VFK23_11760"
     CDS             3085..3303
                     /locus_tag="VFK23_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HET7319805.1"
                     /translation="MEKVLIAASAALAIGLTAIATAWAQSKIGSAGAGALAEKPELTG
                     TIVILVAIPETMVILGFVVATMILFTVK"
     gene            3305..3895
                     /locus_tag="VFK23_11765"
     CDS             3305..3895
                     /locus_tag="VFK23_11765"
                     /inference="COORDINATES: protein motif:HMM:NF014093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HET7319806.1"
                     /translation="MSSQELIESLRHAAEEKARLMREETEREAEDLRLAVSRKVEDLR
                     RRYADRLAAVSREEARNALAEAGRRARAARLSAEKTLSDRLFLLARSSLSRLREDGYP
                     AVFEKLAAELPPMSWKVVRVHPADVVLARKYFPDAEIVPLEAVTGGVEVEVDGGKIRV
                     VNTFEKRLERSWDDLLPHLIRDVYKEVPDGTSPESG"
     gene            3870..4835
                     /locus_tag="VFK23_11770"
     CDS             3870..4835
                     /locus_tag="VFK23_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HET7319807.1"
                     /translation="MELLQNPADRGYPADYLLARIKGRRAGLIKDWRPFIYGTALAGT
                     PLPEKASGLTKAKPRDGTGGILMREYRWVYFQMNRRLREIFYPFFLYAELRTIFACLR
                     RLKDKKAGALDDLLGHSLLSDAVKKVLLVNDDLKHAVAGLERLFLSLSPGFAGLTGVL
                     ETGGLRGVEQRLTERFLAEAAGFSPHPVMKTFFSRIIDARNILSLAKFLRFELKTAPA
                     PIPGGTIPGARLREAARTKNLATLEALVNEAAGVRPERPEPPQVERALYQGMTKWLKR
                     TGRDPFGAAPILDYLWRCSIEAMNMNVLYDGRDVERELIAAELVR"
     gene            5024..5344
                     /locus_tag="VFK23_11775"
     CDS             5024..5344
                     /locus_tag="VFK23_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HET7319808.1"
                     /translation="MKSIVFITPRDAAYGPGLSGVSQHAVEAGDAEQTLLKVLAEQAS
                     GLVVIDERFLTVVPADRLRELEQAWNGVLLVLPSPEKPPAEVEDYAARLIRRAIGYHV
                     RLNV"
     gene            5341..7101
                     /locus_tag="VFK23_11780"
     CDS             5341..7101
                     /locus_tag="VFK23_11780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HET7319809.1"
                     /translation="MTISHSNRSDYESAQAGGTGLDKEMPARSGRILGISGPTVSTDL
                     KGLKLYERVYVGQALLTGEVVRLEKDKAVVQVYEDSRGLAVGEPVKGVGMPLTVRLGP
                     GLLGGMFDGLQRPLERLRAEMGPFITGGKEMYALDYMKRWNFSPAKKPGDEVRPGDVM
                     GTVQEGQFMHYIMAGLDESGTIERIVEGDVSLDEPLGEYAGGREIPGCHEWPVRIARP
                     FRRKFAPHEPLITGQRSIDFLFPLARGGTAIFPGGFGTGKTILEQAVAKFADVDIVVY
                     VGCGERGNEMADMIAEFEELKDPWTKRPLRERTVLVVNTSNMPVAAREASIYTAVTMA
                     EYYRDMGYHVLFLADSLSRWAEALREISSSLEEMPGEEGYPTYLASRLSGFMERAGVV
                     ETLAGRTGSLSMILSVSPPGGDFTEPVTQACLRTTGSFLMLDTALAHRRHYPAINWFQ
                     SYSLYGAEVAGHYRTAVSPEWEGLLRRCREMLQQEEALREVAEIVGAEGLQNQDRLLM
                     RTAEMIRTEFLQQNAYSDDAFSPPEKTFATMKRIIDFFDAASARLKEGASLEEAMKGQ
                     ESGDGSQKSGVGNVKQGAAS"
     gene            7161..>7383
                     /locus_tag="VFK23_11785"
     CDS             7161..>7383
                     /locus_tag="VFK23_11785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014873.4"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HET7319810.1"
                     /translation="MRLSEHIYRTISTVAGPLLFVQNVQSARIGEVVTVHAPDGRRME
                     AEVLEIEGDTVLVEVYGTTQGLDIERTEVS"
BASE COUNT         1472 a         2163 c         2313 g         1435 t
ORIGIN      
        1 cttcctgcgc cacggcggag ggtttgctgt ccgccatcct cggcgttctc cggacatcgg
       61 tccccgggac ggcgtagctg aactcgatgg ggatgccgtt cggatcatag gagtagatcg
      121 agtggatgaa gccgtggtcg atgacctccg acacccagat gcccgcagcg ttgatccggt
      181 ccttgagggc ccagaggtcg tcctcggtct cgacgccgaa ggagacgtgg tcgaagacga
      241 aaggcccctt tgccggcctg ccgtgttcct tttccacgac cggctccacc gcgggccatt
      301 cgaaaaaggc tatcatgtcg tgcgccgata tctcgaagaa gtaatggcgg tagcccggct
      361 ttcccaggcc tgcaataagc ctcattccga gcaggtcccg ccagaagcgg atggtctcat
      421 ccatatcccg cgtggccatg gccaggtggt tcactccgtt gtaggtcggc atctctcctc
      481 ctttgtcgaa gggaaataat actccaagtt tacctcaatg aaaaggcgcc ggtggaaaga
      541 acagggaacc ggctggcgcc gtgttctccc tacagacaga atattgcagg ggttgcggac
      601 tgctaaagtc ccgtttcttt gctacaatgt aattgtgcag caccgaaaaa actccgtttc
      661 ccatgacaat gaaaaccgaa acttatcaac ctctcaatcg tcgtctctgc cgttgaattc
      721 ctcccccgtg acaggcttcg ggaggaactc cggtgaaagg gcgctttcag atggaaaatc
      781 acacgctgcg cgatatcatc gaagtcgaaa aggagatcca gaaatccctc gatagcacga
      841 aagaaacgct gaatgattgg ctcgatgccc gcaaaaaaga ggcggaggaa gagctggcgc
      901 gaagcgagca ggaacttctc gcttcctttc agcaatcccg ggaacaggtg atgcgggatg
      961 ccggcggcag ggcatccgtg ctcgcgacgg cggcggagga gcgggccgcc cggatgacag
     1021 agcttcagaa cgacctgctg gcgggtatcg tggcaggcca tattaagaag atcctgccgg
     1081 gatgaatcat gatcgttcgg atggcaaaga tcgagattgt gggcgcgaaa gagctgcttc
     1141 aggacgtgct ctccaggctt agggatctcg ggatatttca gatagagccg gtggcggtcg
     1201 gtattctgga aaacggaaag atagacgacg atatccgctc tttcaccctc gatgaaaaga
     1261 cgatgttcga gcgggttttt ctcgaggagc tgcgcacaaa ggtgggaaat cttctttccc
     1321 gcctgccaaa gcttccggtc aggaagagct atctggaccc ccgtccggtg atcgatactc
     1381 tgtcggcgat cgtcgagcgt cataccgcgg cagtcgcggc acgcaacgaa cgcagcgagg
     1441 ttcttcgcaa agaacgggct gaactggaac gatacgccct ctttctcgga gcgctggcgc
     1501 cgctcgtcgg cagcgccaga gagacgccgg acctcgaatt catcggcctg accatccggg
     1561 agcccgacat ggtcggccgt ctcaaagagg cgatttcgcg catgacggac tggaaatatg
     1621 aactggtgac ggaggccgcg aaggacggaa cgctcgtagg tctcattacc gttgaaaaag
     1681 agcttgccgg cgtcgtgaaa aagaccttga gcgacgagca ggtcctggaa ctcgtcttgc
     1741 ccccgacctt cagcggcttc agtttttccg aaaaggtctc ctacgtccag aagcggatcg
     1801 cagaggtgac gcgggagatt gacgccatcg aggcgtcgct tgcccagttc gcctgtcgct
     1861 ggatcccgat ctaccggagc gtccgggagt ggatcgatga ccggttatcg ctcctttccg
     1921 ccacgacagc ggtcttcgag acgcgcatgt gctttttcat caccggctgg atgccgcaga
     1981 gggacgtggc ggccgtccga ggcaccctga aggacgtcta ccaggggaac atagtggtgg
     2041 cggagaagga gattcgcgag gaagacatcg accgggtccc gatcattctc aagaacccgc
     2101 cttacttcag gcccttcgag atcttcacga gcctgttgcc gctgcccgcc tatacctcct
     2161 atgatccgac gcccttcatc gggctcttct tccccatctt cttcggaatg atactgggcg
     2221 acgcggggta cggactgctt ctcgcgctgc tcgcagtctt cctgaagcgc aggtaccggc
     2281 agcagaaata cgtccgggac ggcgccgcca tccttctcgt cgcatcgctg tactcggtct
     2341 ttttcggggt gctgttcggc gaattcttcg gggacctgcc ggagcgcctt tttcacctgc
     2401 agccgatatg cgtggagcgc cgcaccgcaa tcgtgcccat gatctttttc acgctcgccg
     2461 tgggcgtggc gcacgtgctc ctcggcctgt tcttcggcgt cgtcacgggg cttcgaaaaa
     2521 aggaaaagaa ggaagctgcc tacaagctca tcagcatcgt catcatactg ggaataattt
     2581 tcgtcctgtc ctccctgtcc ggcgtgcttc cgtcggtgct ggcccggccg atcatcatcg
     2641 ccatcctctt cctgacgccg ctcctcttct tcacgggcgg gatcctggcc cccctggaac
     2701 tcctgaaaag catcggcaac atcatctcct acgtccgtat catggccatc gggctcacgt
     2761 cggtcctgct cgcctttgtc gcgaacaatc tcggcggcct gaccggcgat gtcgtgaccg
     2821 gcatcgtcgt ggcggggctc atccatttcc tgaacatcat cctcggcgta ttctcgccga
     2881 ccatccattc gttgaggctc cattacgtag agttcttcag caaattcatg gaacatggag
     2941 ggaggaggtt cgagccgctg aagagatgaa catgaatagg agagggggcg agggggcgaa
     3001 tcggcgatag agacaaaaaa tctttcttcg tttcgcgcac tcgcccattc cccgattccg
     3061 ttcatacaac aaaggaggcc atacatggaa aaagtcttga tagcagcgag cgccgctttg
     3121 gcgataggac ttaccgccat cgcgaccgcc tgggcgcagt cgaagatcgg gtcggcaggg
     3181 gcaggggcgc tcgccgagaa gcccgaactc acgggcacga tcgtgatcct cgtcgccatt
     3241 cccgagacta tggtgatcct cggattcgtg gttgccacga tgattctctt cacggtgaaa
     3301 taggatgagt tcccaggaac tgatcgaatc gctccgtcac gccgccgaag agaaggcccg
     3361 cctcatgcgc gaggagacgg agcgggaagc ggaggacttg cggcttgcgg tgtcccgaaa
     3421 ggtcgaggac ctgcgcaggc gctatgccga ccggctcgcg gccgtttcgc gggaagaagc
     3481 gcggaacgcc cttgccgagg ccggacgccg cgcccgggct gccaggcttt ccgcggagaa
     3541 aacgctgtcc gaccgcctgt ttctgcttgc ccgctcttct ctcagccgtc tgcgggaaga
     3601 tgggtatccc gcggtttttg agaaactcgc ggccgaactg ccccccatgt cgtggaaagt
     3661 cgtgcgggtg catcccgcgg acgtcgtcct ggcccggaag tatttccctg acgccgagat
     3721 cgtcccgctt gaggccgtca cgggaggcgt ggaagtcgag gttgacggcg ggaagatcag
     3781 ggtcgtcaat acgttcgaga aaagactgga gcggtcgtgg gacgacctgc tcccccattt
     3841 gatcagggat gtctacaaag aggtgcctga tggaacttct ccagaatccg gctgaccggg
     3901 gatatccggc tgactacctg cttgcgagga tcaagggcag gcgcgccgga ttgatcaagg
     3961 attggagacc gttcatctac ggcacggccc tggccgggac gcccctgcct gagaaggcga
     4021 gcggcctgac gaaggcgaaa ccgcgggacg ggaccggagg aattctcatg cgggaatacc
     4081 gttgggtata tttccagatg aaccggcggc tccgcgaaat attctacccc tttttcctgt
     4141 acgccgagct caggacgatc ttcgcctgcc tcaggcgtct caaggacaag aaagccgggg
     4201 cgctcgacga tcttctcggt cacagcctgc tctccgatgc tgtaaagaaa gtccttctgg
     4261 tgaacgatga tctcaaacat gccgtcgccg gtttggaacg tctcttttta tcgctttcgc
     4321 cgggtttcgc cggcctgacc ggcgttcttg agaccggagg gcttcgcggg gttgaacagc
     4381 gcctgacgga acggtttctt gcggaggccg cgggcttttc gccccatccc gtgatgaaaa
     4441 cttttttctc gaggatcatc gacgcacgga acatcctgag cctcgcaaag ttcctgcggt
     4501 ttgagctcaa gacggccccg gcgccgattc ccggcggcac gatcccgggc gcgagactga
     4561 gggaggcggc aaggacgaaa aaccttgcta ccctggaagc cctggtcaac gaggcggccg
     4621 gcgtccgtcc cgagaggccc gaaccgccgc aggtcgagcg cgccctgtac cagggcatga
     4681 cgaaatggct gaaaaggacc ggcagagacc ccttcggcgc ggcgcccatc ctggattatc
     4741 tctggcgctg ctccatcgag gcaatgaaca tgaacgtatt gtatgacggc cgggacgtcg
     4801 aacgggagct catcgcggcc gaactggtgc ggtaggaacg tttatgccgg gaaaaacaaa
     4861 tggacgcaga taacatcgga ttcacgcaga ttaaaacgag tgaaacctcg ttacagcgtg
     4921 ataaaaagat tttcccggat ggagcgcgca tatccaatcc tcgtgtccgg ttttatccgt
     4981 gtctatccgc gttcatccgc gttccgttgt cggtttcagg attatgaaaa gcatcgtctt
     5041 cataacaccg cgcgacgcgg catacggtcc gggcctgagc ggcgtctccc agcacgccgt
     5101 ggaggcgggt gatgcggagc agacgctcct taaggttttg gcggagcagg cttccggcct
     5161 tgtcgtcatc gacgagcgct tcctcacggt agtgccggcg gacaggctcc gcgaactcga
     5221 acaggcgtgg aacggcgtcc tcctggtgct gccgtcgccg gaaaagcctc cggccgaagt
     5281 cgaagattac gcggcaaggc ttatcaggag ggccatcggg taccatgtga ggctgaacgt
     5341 atgacgatat cgcattcgaa tcgctcagat tatgaatcgg cgcaggcggg cggaacgggt
     5401 cttgataagg aaatgcccgc ccggagcggc aggatcctgg gcatatcggg gccgaccgtg
     5461 agcacggacc tgaagggcct caagctttat gagcgcgtct acgtgggcca ggcattgctt
     5521 accggcgaag tggtgcgtct tgagaaggac aaggccgttg tccaggtgta cgaggacagt
     5581 cgcggtcttg ccgtgggcga accggtcaag ggtgtgggca tgcccctcac ggtccggctc
     5641 gggccggggc ttctgggcgg catgttcgac ggcctgcagc ggcccctgga acggctcagg
     5701 gcggagatgg gcccctttat caccggcggc aaggagatgt acgccctgga ctacatgaag
     5761 cgctggaatt tttcccccgc gaaaaagccc ggcgacgagg tccgccccgg cgatgtcatg
     5821 ggcacggtgc aggagggaca gttcatgcat tacatcatgg ccggcctcga cgagagcggc
     5881 acgatcgaac ggatcgtgga gggcgacgtc agccttgatg aaccgctcgg agaatatgcc
     5941 ggcggccggg aaattcccgg gtgccacgaa tggccggtgc gcatcgccag gcccttccgc
     6001 aggaaattcg cgccgcacga gccgcttatc accggccagc ggagcatcga ttttctcttc
     6061 ccccttgccc gcggcggaac cgctatattt ccgggagggt tcggcacggg caagaccatc
     6121 ctcgagcagg ccgtggccaa gttcgccgac gtcgacatcg tggtctacgt gggatgcgga
     6181 gagcggggga acgagatggc ggacatgatc gccgagttcg aggaactcaa ggacccctgg
     6241 accaaacggc cgctccggga gcggaccgtc ctcgtggtca acacgtcgaa catgccggtc
     6301 gccgcgcgcg aggcctcgat ctatacggcg gtcaccatgg ccgagtacta tcgcgacatg
     6361 gggtaccacg tgctgttttt ggccgacagc ctctcgcgct gggccgaggc gctgcgcgag
     6421 atttcctcgt cgctcgagga aatgcccgga gaagaagggt atcccaccta ccttgcctcg
     6481 cggctctcgg ggttcatgga gcgcgccggc gtcgtcgaga cgctcgccgg caggaccgga
     6541 tcgctcagca tgatcctgtc ggtatcgccc cccggaggcg acttcaccga gccggtcacg
     6601 caggcctgtc tgcggacgac cggttcgttt ctcatgctcg acaccgcgct cgcccaccgg
     6661 cggcattatc ccgccatcaa ctggttccag agctattctc tgtacggcgc cgaggtcgcc
     6721 ggtcattacc ggaccgcggt gtccccggaa tgggaaggac tcctgcggcg atgcagggaa
     6781 atgcttcagc aggaggaggc gctccgtgaa gtagcggaga tcgtcggcgc cgaggggctc
     6841 cagaatcagg accgcctgct catgcgcacg gcggagatga tccgcaccga gttccttcag
     6901 cagaacgcct acagcgacga cgccttctcg ccgcccgaga aaaccttcgc gaccatgaag
     6961 cggatcatcg acttcttcga cgccgcgtcg gcgcggctga aggaaggggc gtcgctggag
     7021 gaggcgatga aaggacagga gtcaggagac ggaagtcaga agtcaggagt tggaaacgtg
     7081 aagcaagggg cggcatcgtg agcgcctgat tgatttttat tcagacttct gtcttctggc
     7141 tcctggattc tggattcacc atgagactct ccgaacacat ataccgcacc atatccaccg
     7201 tggccggccc gctcctgttc gtccagaacg tgcagagcgc gaggatcggc gaggtggtga
     7261 ccgtccatgc gcccgacggc aggcgtatgg aagccgaggt gctcgagatc gagggggaca
     7321 cggtgctggt cgaggtgtac ggcacgactc agggcctcga tatcgagcgc acggaggtgt
     7381 cct
//
LOCUS       DASSLD010000651         2179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_79983,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000651 DASSLD010000000
VERSION     DASSLD010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79983"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFK23_11790"
     CDS             <1..495
                     /locus_tag="VFK23_11790"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HET7319811.1"
                     /translation="TRPVLQGARLTAWELMQEGIPCTLITDNMAGYFMYRDMIDLAIV
                     GADRVARNGDVANKIGTYGLAVLCKEHGIPFYVAAPVSTIDFSIESGADIPIEERDPR
                     EITHVFGKVQIAPAGVKAANPAFDVTPARYIAAIITEKGAFHPKDIHKLAGGTTDPER
                     IRIK"
     gene            693..1787
                     /gene="gcvT"
                     /locus_tag="VFK23_11795"
     CDS             693..1787
                     /gene="gcvT"
                     /locus_tag="VFK23_11795"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET7319812.1"
                     /translation="MKQTVLHLKHEQSKARMTEFQGWQVPLQFSDALEEHHAVRAAAG
                     LFDIGYLGRIEVSGPGAAGLLQKTVTRNLSKVPEGSAQFGCICNPAGGILDDVFVFRL
                     PAGKTTEARWLLTTNALNAEKVLAWLRQHAPQDVQVVDRTGDTAQLALQGPRSLSILE
                     RLAGQHFKKLKTRMVREMVFADTAVLVSRIGYTGEHGYELITPADRAGALWDALIAAG
                     SSEGMLLCGLAARDILRLEMGYLMYGNDIDETRTPIEAGLSFFVDFKKEFIGREALLK
                     IKTEGPKQKLAGFVLLDKGVPKGGGSIFSENREIGVVTSGGHSPQLRKGIGLGYVVSR
                     YSQPGQEIEIEIRDREIAAKIVDLPFYRKK"
     gene            1982..>2179
                     /locus_tag="VFK23_11800"
     CDS             1982..>2179
                     /locus_tag="VFK23_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319813.1"
                     /translation="MDRREFLIGLGIALVAGPAAISLNSCGSSSNSGSPAPANSFSVT
                     SNPDSTGHSHNVFIPFADLTNP"
BASE COUNT          486 a          663 c          636 g          394 t
ORIGIN      
        1 acacgccccg tgctccaggg ggcacgcctg accgcctggg agctcatgca ggagggcatc
       61 ccctgcacgc tcattaccga caacatggcc ggctacttca tgtaccgcga catgatcgac
      121 ctcgccattg tcggcgcgga ccgcgtcgcg cgcaacggcg atgtggcgaa caagatcggc
      181 acctacggac tcgcggtcct ctgtaaggag cacggcatcc ccttctacgt ggcggcgccg
      241 gtctcgacga tcgatttcag cattgagtcg ggcgccgaca tccccatcga agaacgggac
      301 ccgcgcgaaa tcacacacgt gttcggcaag gtccagatag cgcctgcggg agtgaaggcg
      361 gcgaacccgg ccttcgacgt caccccggcc cgatacatcg ctgccattat cactgaaaaa
      421 ggggcgttcc atccgaaaga catccacaag cttgccggcg gaacaaccga tccggaaaga
      481 atcaggatca aatagcaaaa caacgtactg gaatcatgtc attcccgaag gtcaacggga
      541 acactgcata tcgcacgaca gctgacacca cggcgtaact ttattttcat tcccggaatt
      601 ctctatcggg gatacggatt gagacagggg atgaacctgt ccccgcttga aacattcggg
      661 gatgacggca aacacatggc ttagagggcc gtatgaaaca gactgttctc cacctgaagc
      721 acgaacagag caaagcccgg atgacggaat tccagggatg gcaggtcccg ctccagtttt
      781 ccgacgcact ggaggagcat catgccgtgc gcgccgcagc cggcctgttc gacatcggct
      841 acctcggcag gatcgaggtg tccggcccgg gcgccgcggg gctgctccag aagaccgtta
      901 cccgcaatct ctcgaaggtc ccggagggtt cagcgcagtt cgggtgtatc tgcaatcccg
      961 ccggcggcat tctcgacgac gttttcgtgt ttcgcctccc cgccggtaaa acgacggagg
     1021 cccgctggct ccttacgaca aacgccctca atgcggagaa ggtcctcgcc tggctccggc
     1081 aacacgcccc gcaggacgtt caggtcgtcg acaggaccgg ggacaccgcg cagcttgccc
     1141 tgcagggtcc gcgctcgctc tccattctcg aaagactcgc gggccagcac ttcaagaagc
     1201 tcaagaccag aatggtccgg gagatggtgt ttgcggacac ggcggtgctg gtgtcccgga
     1261 tcggctacac gggcgagcac ggctatgagc tcattacgcc cgccgaccgg gccggggcgt
     1321 tgtgggacgc actcatcgcc gccggaagca gcgagggcat gctgctctgc ggccttgcgg
     1381 caagggacat cctgcgcctg gaaatgggct atctgatgta tggaaacgac atcgacgaga
     1441 cccgtacgcc catcgaggca ggcctctcct ttttcgtcga tttcaagaag gaattcatcg
     1501 gcagggaagc gctcttgaag atcaagacag agggcccgaa gcagaaactg gccggattcg
     1561 tgctgctgga caagggtgtc ccgaagggcg gcggcagcat cttttccgaa aaccgggaga
     1621 tcggcgtggt gacgagcggc ggacactcgc cccagcttcg caaaggcatc ggcctgggct
     1681 acgtcgtcag ccgctactcc cagccgggcc aggaaataga gatagaaatc cgcgaccggg
     1741 aaatcgcggc gaagatcgtg gacctgccgt tttaccggaa aaaataaact tccgcgcctt
     1801 tcgccatttt ttctgattca ttcgtccttt cgccgggcgg cttgacatac cccgaattcg
     1861 tcctatactc agtgtagaga cttttccttc cgaaataatt cctgaaaaac cgaacagaac
     1921 ggaaattata acgaaggaaa gccgtgggcg ttgtctgaac agacggcaaa aggaggaaaa
     1981 tatggacagg agagagtttt tgatcggtct cggcattgcg ctggtcgccg gccccgctgc
     2041 gatcagcctc aatagctgcg gaagcagctc aaactcgggg tcccccgcac cggcgaacag
     2101 cttcagcgtg acatcgaatc cggacagcac gggccattct cacaacgtct ttatcccctt
     2161 tgctgatctc acgaacccg
//
LOCUS       DASSLD010000652         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_113220,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000652 DASSLD010000000
VERSION     DASSLD010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113220"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFK23_11805"
     CDS             complement(<1..561)
                     /locus_tag="VFK23_11805"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7319814.1"
                     /translation="MHDLAITGVYFFCIAVLYSTVGHAGASGYLATMALLSFPAGVMK
                     PTALALNIIVALVTTIRFAMAGYFSWRLFWPFAFASVPLAYLGGGISMDATIYKILVG
                     VALVFAAAHLILRSNVAADDPGPIAYPGIAGSAAVGGGIGFLSGLTGVGGGIFLSPVL
                     IILHWAGLRRTAAVAAAFILVNSISGL"
     gene            complement(604..2109)
                     /gene="nifK"
                     /locus_tag="VFK23_11810"
     CDS             complement(604..2109)
                     /gene="nifK"
                     /locus_tag="VFK23_11810"
                     /EC_number="1.18.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016612 - molybdenum-iron nitrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016163 - nitrogenase activity [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase molybdenum-iron protein subunit
                     beta"
                     /protein_id="HET7319815.1"
                     /translation="MKVRDHSELFKQKDYQEQFERKKEFENCWPAEKVKEVSEWTKTE
                     EYKELNFARQHIKINPAKACQPLGAVFCASGFEGSMPFVQGAQGCVAYFRSHLSRHFK
                     EPFAAISTSMTEDAAVFGGLNNMLEGLENAYTLYKPKMILVSTTCMAEVIGDDLNAYI
                     RTAREKGVIPQDLPVPFAHTPSFVGSHIVGYDNMMKGILSTLSTGKKGEPNKKINIIP
                     GFDTYTGNYHEIKRLMAMMGIELTLLADESDTFDSPNTGEYKLYPGGTPLADAMDAVN
                     APTTVALQKYSTMKTVDFIQKEWSQKTLTLLPIGIKNTDHFFEELSRLTGKPVPASLE
                     AERGRAVDAMVDSHPYVHGKRFALAGDPDMLLGLTSLIMEMGGEPVHIVCTNGDKSFE
                     AEANELLASSPFGTKGKVYAGKDMWHLRSLLFTDPVDLLIGNSYAKFLTRDTGTPLIR
                     IGFPLFDRHHLHRQPIIGYQGVINLITMMVNTVLDELDRKTMDSASFDVIR"
     gene            complement(2217..>2421)
                     /locus_tag="VFK23_11815"
     CDS             complement(2217..>2421)
                     /locus_tag="VFK23_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HET7319816.1"
                     /translation="VHELKPDLVASGIKEKYVFQKMGLPFRQMHSWDYSGPYHGFDGF
                     PVFARDMDMTVNSPTWGLIRRKK"
BASE COUNT          498 a          725 c          683 g          515 t
ORIGIN      
        1 taaaccggat atcgagttga cgaggataaa tgccgcggcc acggccgccg tcctccggag
       61 gcctgcccaa tgcaaaatga tcaggaccgg gctgagaaag atgcctccgc cgaccccggt
      121 aaggccggaa aggaacccga tgccccctcc cactgccgcc gacccggcaa tccccggata
      181 tgcgatcggt ccgggatcat ccgcagcaac attactccga aggataagat gtgcggcagc
      241 gaacactaat gcgacgccca cgagtatctt gtaaatcgtc gcgtccattg aaattccgcc
      301 gcccaggtac gccaggggaa ccgatgcaaa agcaaagggc cagaacaacc gccaggaaaa
      361 atatccggcc atggcgaacc ggattgtcgt gaccaaggca acgatgatat tcagcgcaag
      421 cgccgtcggc ttcatgacgc ctgccggaaa agacaggagc gccatggttg cgagatatcc
      481 cgaggccccc gcgtggccga ccgtggaata gaggacggcg atgcaaaaga aatatacgcc
      541 tgtgattgcg aggtcgtgca tggcttcaca ctccgagctc agaactccaa actccgaacc
      601 gctctaccgg atcacatcaa agctcgccga atccatcgtc tttctgtcca gttcgtcaag
      661 cacagtattc accatcatcg ttatcagatt gatgacgccc tgatacccga tgatcggctg
      721 gcggtgcagg tggtgccggt cgaagagcgg gaacccgatc ctgatgaggg gcgtcccggt
      781 atcgcgcgtc aggaacttcg cgtaggagtt tccgatcaaa agatcaacag ggtccgtgaa
      841 cagaagcgag cgcaagtgcc acatgtcttt ccccgcatag accttgccct tcgttccgaa
      901 ggggctcgac gcgagaagct cgttcgcctc ggcctcgaaa cttttatcac cgttcgtgca
      961 cacgatatgg accggctcgc cgcccatttc catgatgagg ctcgtcaggc cgagaagcat
     1021 atcggggtca cccgcgagcg caaagcgttt gccgtggacg tagggatggg agtccaccat
     1081 ggcgtccact gcccgtcctc gctccgcctc gagcgatgca gggaccggct tgccggtgag
     1141 cctggaaagc tcctcaaaaa agtggtccgt gttcttgatc ccgatgggca agagcgtaag
     1201 cgttttctgg ctccactcct tctggatgaa gtccacggtc ttcattgtcg aatatttctg
     1261 gagggcgacc gtggtcggcg cattcaccgc gtccatcgca tcagcaagcg gcgttccgcc
     1321 gggatagagc ttgtactcgc ccgtgttcgg cgaatcaaag gtgtcgctct cgtccgcgag
     1381 gagcgtgagt tcgatgccca tcatcgccat gagccgtttt atctcatggt agtttccggt
     1441 gtacgtgtcg aagcccggga tgatattgat ctttttgttc ggttccccct tcttgcccgt
     1501 ggagagtgtc gagaggatgc ctttcatcat gttgtcgtag cccacgatgt gggagcccac
     1561 gaaactcggc gtatgcgcaa acggcaccgg caggtcctga gggatcacgc ccttctcgcg
     1621 cgcagtcctg atataggcgt tcaggtcgtc gccgatcacc tccgccatgc aggtcgtcga
     1681 gactaagatc atcttcggct tatacagggt ataggcgttc tccaggcctt cgagcatgtt
     1741 gttcaggccc ccgaacaccg cggcgtcctc ggtcatcgaa gtcgagatcg cggcgaaagg
     1801 ctccttgaag tgccgggaga ggtggctccg gaagtaggcc acgcagccct gtgcgccctg
     1861 cacgaacggc attgagcctt caaagcccga ggcgcagaac accgcgccca ggggctggca
     1921 ggccttggcc ggattgatct tgatgtgctg gcgcgcgaag ttcagctctt tgtattcctc
     1981 ggtcttcgtc cactccgaga cctctttcac cttctcggcg ggccagcagt tctcgaattc
     2041 cttcttgcgc tcgaactgct cctggtagtc tttctgtttg aacagctcgc tgtggtctct
     2101 gactttcatc atatcctcca aaaaccatgg gacgcagatt ttcgcagatg aacgcagatt
     2161 taatttttcg aatccctgga tttatccgcg tcaatctgcg tcaatccgca tccaaactat
     2221 ttctttcttc tgattaggcc ccacgtcggg ctgttcacgg tcatgtccat gtcccgcgcg
     2281 aagaccggga acccgtcgaa gccgtggtac gggcccgagt agtcccacga gtgcatctgg
     2341 cggaacggca gccccatctt ctggaagacg tacttctcct tgatcccgga ggcgacgagg
     2401 tcgggcttca gctcgtgcac g
//
LOCUS       DASSLD010000653         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1376334, whole genome shotgun sequence.
ACCESSION   DASSLD010000653 DASSLD010000000
VERSION     DASSLD010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376334"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..452
                     /locus_tag="VFK23_11820"
     CDS             261..452
                     /locus_tag="VFK23_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319817.1"
                     /translation="MAILNMKSTDPAGTGRGKEALHEHAGTEHDETCRCKEASKMTPG
                     QLLRTMFNDLAFWKKTKKD"
     gene            539..1135
                     /locus_tag="VFK23_11825"
     CDS             539..1135
                     /locus_tag="VFK23_11825"
                     /inference="COORDINATES: protein motif:HMM:NF021573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2301 domain-containing membrane protein"
                     /protein_id="HET7319818.1"
                     /translation="MGSEQVFQPLTREDRITVALYRGGIVMSTIIVSYAAFLLAGASS
                     HAADAHIGRAADILAWGLYASVGMSVFFIHLYVGKFKRFLITLYLLGLCGLIALLYIG
                     RGSVSAALLTTPYATLLLLPLSGCLGFVTAKEAFCFRLFEGYLLAMIMPVYLLLVSLT
                     GQGASSGLTVIAVMLVIFTLRKVFMPAAYDIGDKTAYR"
     gene            1287..1823
                     /gene="pgsA"
                     /locus_tag="VFK23_11830"
     CDS             1287..1823
                     /gene="pgsA"
                     /locus_tag="VFK23_11830"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET7319819.1"
                     /translation="MNLPNYITLVRIVLIPFFVNLMIYGYYRSALLVFAVACVTDALD
                     GMIARLTGQITELGAFLDPMADKLLIISAFVTLVLKSMLPVWLVIVVVSRDVILVLGA
                     VAIYFTGHRFTARPSLIGKATTVLQLVTVTLALVLTNYGAGSGQMAALHLTTAAFTLA
                     SGIQYVTRGTRIVGHNQE"
     gene            1845..>2146
                     /locus_tag="VFK23_11835"
     CDS             1845..>2146
                     /locus_tag="VFK23_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319820.1"
                     /translation="MKISEMIRKALEKKGFKNLKDASKVLGISPELLRVTINKGHIPK
                     DKTLIQIAHKLSLDKSVLILAAHQEKVPAEVKGFFLSPSQAKFRRGKRKYPLSEEQ"
BASE COUNT          505 a          620 c          602 g          419 t
ORIGIN      
        1 ctcatcgaga gaactggagg aaaccatgaa caagaatcgc acaaccgccg ggacggctga
       61 catcaaggga cctaccgcag gaagctcgtc gaacaccggg agtacatcgc gccgtacgga
      121 caggacatgt tcgagatccg ggaatggacc tggggcgctc cgggagaagc cgaagagaat
      181 gacggcaatt gcatgcacga agaaaacgga ctttgacagg gcacagcccg atgggttaca
      241 atgattaaga ggagcgagga atggcgatac tcaacatgaa gtccactgat cctgccggga
      301 cgggacgcgg gaaagaggcc ttgcatgagc atgcggggac ggagcatgat gaaacgtgcc
      361 ggtgcaagga agcgtcgaaa atgacgcctg gtcaacttct ccggaccatg ttcaacgacc
      421 tggcattctg gaaaaagaca aaaaaggatt gatgctggac cggcaggccg cgacaggcgc
      481 cgggtcattt cgcacaaacg taccgagacg cggaaacaac agaacggaga gaaggtcgat
      541 gggttctgaa caggtgttcc agccacttac cagggaagac aggataacgg ttgcgctcta
      601 tcgcggcggc attgtgatga gcacgatcat cgtaagctac gcggcgtttc tccttgcagg
      661 cgcctcttcg catgcggcag acgcgcacat cggccgcgcg gcagacatcc tcgcctgggg
      721 cctctacgca tccgtaggta tgagcgtttt cttcatccat ctctacgtcg gcaaattcaa
      781 gcggttcctg ataaccctct atcttctcgg cctgtgtggt ctcatcgctc tcctttacat
      841 cggaaggggg tctgtgtcag ccgcgctcct cactacaccg tacgccacct tgctcctgct
      901 gccgctctcc gggtgcctgg ggttcgtgac cgcgaaagag gccttctgct tcaggctctt
      961 cgagggctat ctccttgcca tgatcatgcc ggtctacctc ctgctcgttt ccctcacggg
     1021 ccaaggcgcc tcctccggcc tcaccgtgat cgccgtcatg ctcgtcatct tcacgctgcg
     1081 aaaagttttt atgcccgccg catacgacat cggcgacaaa accgcctacc gctgatccag
     1141 aaatccgtcc gcccttcaat cgcagcccct tgagcgccga cactgtaaca aaaacaaatg
     1201 gtaacaaaaa gataaaggtt gacatcacaa ataaacatgt ttattatgtc ttccggttga
     1261 agaacacaac cgaggacggg gacccgatga accttccgaa ttacataacg cttgtccgga
     1321 tcgtcctcat tccgttcttc gtcaacctga tgatctacgg ctactaccgg tccgcgctcc
     1381 tggttttcgc cgttgcctgc gtaaccgatg ccctggacgg aatgatcgcc cggctcaccg
     1441 gccagataac cgaactcggg gcgttcctcg atccgatggc cgacaaactc ttgatcattt
     1501 cagccttcgt cacactcgtc ctcaagagca tgctgccggt ctggctggtc atcgtcgttg
     1561 tgagcaggga cgtcatcctc gttctcgggg ccgtcgcgat atactttacc gggcaccggt
     1621 tcactgcccg gccgtccctc atcgggaagg cgacaaccgt actgcagctg gtcacggtga
     1681 cgctggcact ggttttaacg aattacggcg cggggtcggg gcagatggcc gcattgcact
     1741 tgaccaccgc agccttcacg ctggcatcgg gcatccagta tgtcacacgg gggacgcgga
     1801 tcgtggggca caaccaggaa taagaacatc accgggagga aagaatgaag atttcagaaa
     1861 tgatcaggaa ggcccttgaa aagaaaggat tcaagaacct gaaggacgcg tcgaaggtcc
     1921 tcgggatcag ccccgagttg ctgagggtca cgatcaacaa ggggcacata ccgaaggaca
     1981 agacgctcat ccagatcgcg cacaaattga gcctcgacaa gtcggtcctg atcctggcgg
     2041 cgcaccagga aaaagtaccc gcggaggtca agggtttttt cctgtcgccg tcccaggcaa
     2101 agttcaggcg gggcaagcgc aagtacccgc tttcggaaga gcagat
//
LOCUS       DASSLD010000654         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_602697,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000654 DASSLD010000000
VERSION     DASSLD010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602697"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(427..1629)
                     /gene="rpsA"
                     /locus_tag="VFK23_11840"
     CDS             complement(427..1629)
                     /gene="rpsA"
                     /locus_tag="VFK23_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET7319821.1"
                     /translation="MDTDDKAANTEDSGESFAELFEKSFKKQARLEPGQMVEAQIVKI
                     SGEWIFLDLGGKGEGYLDKKEMMDEAGNLSVREGDTVRAYFVASENNEMHFTAKIGSG
                     PGKQNQLEDAWRNGIPVDGTVAKEMKGGFEIRIAGSVRAFCPFSQMGLRRGEPQAEYI
                     GRSLPFKIIEYGEGGRNIVVSRRPILDQERRDKVAALKATLKEGMKVNGTVTSIQNFG
                     AFVDIGGVEGLLPVSEIAWTRTNKVSDILSVGQTVEVIIKKLDWEQNRFSFSLKDAMP
                     DPWERVADAFPIGSYHSGTVSRLAPFGAFVTLKEGVDGLIHISKLGAGKRISHPREVV
                     KEGQAVEVKVEAVDPAQRKLSLSLAEISRAEEEAAADLKQYQQSTSTPQNLGTFGELL
                     KGRTGTKE"
     gene            complement(1736..>2421)
                     /locus_tag="VFK23_11845"
     CDS             complement(1736..>2421)
                     /locus_tag="VFK23_11845"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET7319822.1"
                     /translation="GVEWVRLMYAYPGRIGDGLMDVIAGEEKVCKYLDIPIQHFDDKV
                     LAAMNRRGSSADIRKTIERLRKRVPGIALRTSLIVGFPGETDAAFKRLLSFVKEAEFD
                     RLGVFTYSPEEGTAAHGLEPRVPADVAADRYHAIMKAQAKISLRKNHALIGATLRVLI
                     DGMEDMALTGRLMSQAPEIDGMVYLSETEASAGEFVDVTITDATEYDIMGKAANKQKH
                     LPQRSQRKH"
BASE COUNT          436 a          754 c          678 g          553 t
ORIGIN      
        1 aaactgacca ttgaaacaaa atgcagacgg cggggccatt tgaccccgcc gttgttattc
       61 tcctgcttgt ccgactcatc tcaccgaaca ttcccgcact tcccgtaccg tcaccaactc
      121 cccgatgatc cgccgcccga agcagccggc tgcagcggtt gtgtgaactg accgcatggg
      181 gtgatgccat tataatgata aaatgggccg tgcgcaatta ctacgccatt gtagcatggc
      241 gattgattgt tgtgacctac ccggacgggt aggtgttgat gacgggggaa aaatcgggtg
      301 aacgggattg gagaggatac tgtcttgatg agctattcgt ttgcagtgat gaggtgcgcg
      361 gcgagatcgc gcgcccgcag gtgagcgggc gcgcgggatg caggggccgg acgggcgttc
      421 cgattgttac tcttttgtcc cggtcctgcc cttcaggagt tctccgaagg tccccaggtt
      481 ctggggcgtt gacgtcgact gctgatactg cttcagatcg gccgcggctt cctcctccgc
      541 gcggctgatc tcggccagag aaagcgagag ctttctttga gccggatcga cggcttcgac
      601 cttcacctcg acggcctggc cttccttgac cacctcgcgc ggatggctga tccttttgcc
      661 ggcgcccagt ttcgagatgt ggatgagccc gtccacgcct tctttcaagg tcacaaacgc
      721 gccgaagggc gcgagacggg aaacagtccc cgaatggtag gagccgatcg gaaacgcgtc
      781 cgccaccctt tcccacgggt cgggcatcgc gtctttcagg ctgaaggaga agcggttctg
      841 ctcccaatca agcttcttga tgatcacctc cacggtctgc cccacggaga ggatgtcgct
      901 caccttgttg gtccgggtcc aggcgatttc ggagacgggc aggagccctt cgaccccgcc
      961 gatatccacg aacgcgccga aattctggat ggaggtgacg gttccgttca ccttcattcc
     1021 ttccttcagc gtcgctttga gcgcggccac tttgtcccgc cgttcctgat ccaggatggg
     1081 tctgcgggaa acaacgatgt tccggcctcc ctcgccgtac tcgataatct tgaagggcag
     1141 ggaccggccg atgtattcgg cctgcggctc tcccctgcgc aggcccatct gggaaaaggg
     1201 acagaacgcg cgcacgctgc ccgcgatcct gatctcgaat cctcccttca tctccttcgc
     1261 caccgttccg tccacgggaa tgccgttccg ccaggcgtct tcgagctggt tctgctttcc
     1321 gggtcctgac ccgatctttg ccgtaaaatg catctcattg ttttccgagg ccacgaagta
     1381 ggcgcggacg gtgtcccctt ccctgaccga aagattaccg gcctcgtcca tcatctcttt
     1441 cttgtcgaga tatccctctc ccttgccgcc gaggtccagg aagatccact cacccgagat
     1501 cttcacgatc tgcgcctcga ccatctgacc gggctcgagg cgggcctgtt tcttgaaact
     1561 tttttcgaag agctccgcga aactctcgcc gctgtcttct gtgtttgctg ctttatcgtc
     1621 ggtatccata aggtcccttt cagtattaag tataaatttt ttaccacaga ggccacggag
     1681 aaaaaaaacg gtgacggacg gaagaaatca aacaaattct caacctacct ttgatttaat
     1741 gctttctctg tgatctctgt ggcaaatgct tttgcttgtt tgccgccttt cccatgatat
     1801 catattccgt cgcgtcggtg atggttacgt cgacgaattc tccggccgac gcctcggtct
     1861 cggaaaggta caccatgccg tctatctcgg gcgcctgcga catcagcctt cccgtgagcg
     1921 ccatgtcctc catgccgtcg ataagcaccc gcagcgtggc gccgatcagg gcgtggttct
     1981 tcctgagcga gatcttcgcc tgggccttca tgatcgcgtg gtaacggtcc gcggcaacgt
     2041 ccgccgggac gcggggttcg agcccatggg cggccgtccc ttcctcgggc gaataggtga
     2101 acacgccgag gcggtcgaac tcggcttcct tgacgaaaga caggagccgc ttgaacgccg
     2161 cgtcggtctc cccggggaat cccacgatga gagaggtccg gagcgctatg cccggcacgc
     2221 gcttccggag ccgctcgatc gtcttccgaa tgtccgcgga cgatccgcgc ctgttcatgg
     2281 ccgcgagcac cttatcgtcg aagtgctgga tcgggatgtc caggtacttg cagaccttct
     2341 cctccccggc gatcacgtcc atgagaccgt cgccgatccg tccgggatag gcatacatga
     2401 gcctcaccca ctctacgccc g
//
LOCUS       DASSLD010000655         3247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_274228,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000655 DASSLD010000000
VERSION     DASSLD010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274228"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VFK23_11850"
     CDS             <1..924
                     /locus_tag="VFK23_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7319823.1"
                     /translation="HAPAMAVKMRREVLDRPTFSTTELNELVSFLYMLPYLGEPGDAR
                     RGEALLESKSCFKCHSLGKKGRREGTPLDSLAVYQSTVVLLQRMWNHGPEMIGRMTTT
                     GTSIPTFSGNEMADIFSALTKETPDKSKKVFLGVGNAENGEKLFTDKGCIKCHSIFGK
                     GGKRAPDLGKTVAQSNVTKLTTQIWNHVGRMRQLYNQEKLQWPYFAETEMNDLIVFLY
                     SLNYLDKPGDAAKGEKIFSQKKCIGCHFKTEENKQKLLGALKAINTTQFAAELWNHTS
                     AMEAAMVTQGVPWPEMTGAQLRDVLAFLQKQ"
     gene            complement(1026..2318)
                     /gene="nrfD"
                     /locus_tag="VFK23_11855"
     CDS             complement(1026..2318)
                     /gene="nrfD"
                     /locus_tag="VFK23_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET7319824.1"
                     /translation="MQKVWAFLRGCFLLLMKGSKLYYAWLTALLVLIAIGVFAYANQM
                     YNGLIVTAMRDQVSWGFYISNFTFTVGVAAAAILLVIPAYVYHWKPIKEIAILGELVA
                     VSAMVVCLLFVTVDIGRPDRFWHLIPKIGLLNFPQSLLAWDVVVLNTYLVLNFAIASY
                     MLYCYYYRREPNMKYVFPLLMFSIPAAISIHTVTAFLYNGLAARPFWNASILAPRFLA
                     SAFCSGPAGMILLFQVVRKWTNFDIKDKAIFKIAELIAYAMFINLFLLGAELFKEYYS
                     DTVHLAPVKYLYQGLEGHSDLVSYIWTATIFNISGFFLFLIPQTRENFVTLNIGCVLI
                     FIGVYIEKGMGLVIPGFVPDVLGDFYEYSPTSTEMLVSIGIWATGLLVYTLLLRIAVP
                     IMNGDFHVGEDGVKPHIGDQLFLVRKRAVVEEEDLDSP"
     gene            complement(2338..3177)
                     /locus_tag="VFK23_11860"
     CDS             complement(2338..3177)
                     /locus_tag="VFK23_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7319825.1"
                     /translation="MVLPAKDASASVWESFFQKNFRELGKSERRAIMARLEKEYSAKY
                     KKPVRVKDTSPIEGVLYGYGLDLSRCVGCRRCVYGCVTENNQSRDPQIHWIKVLRLEK
                     ERGVDLDHSEQYYNPATVPEEGYFYMPVQCHQCENPPCTKVCPVQATWKEIDGIVVVD
                     YNWCIGCRYCMAACPYGARHFNWKTPGLPAGDMNPETHYLGNRPRPVGVVEKCIFCMQ
                     RTREPNGEYPACVEVCPVGARKFGNLLDPTSEIRNVIENKRVFILKEDLNTQPKFYYF
                     YSV"
BASE COUNT          787 a          932 c          897 g          631 t
ORIGIN      
        1 catgcgccgg caatggccgt caaaatgagg cgcgaagtgc tggaccgccc caccttcagc
       61 acgaccgaac tgaacgaact cgtctccttt ctttacatgt tgccgtatct tggagaacct
      121 ggtgatgccc ggcgcggcga ggcccttctc gagtcaaaat cctgtttcaa gtgccactcc
      181 ctgggcaaaa aagggagaag ggaaggaacc ccgctggatt ctcttgccgt ttaccaatcc
      241 actgtcgtcc tcctgcagag aatgtggaat catgggccgg agatgatcgg ccggatgacg
      301 acgaccggca cttccatccc tacattctcc ggtaacgaaa tggcggacat cttctccgca
      361 ctgacgaaag agacaccgga taaatccaaa aaagtatttc tcggagtcgg gaatgccgag
      421 aacggggaga agctgtttac cgacaaaggt tgcatcaagt gccactcgat cttcggaaag
      481 ggcggcaagc gtgccccgga ccttgggaaa accgtcgccc agtcgaatgt gacgaaactt
      541 acgacccaga tctggaatca tgtcgggcgg atgaggcaac tttataatca ggaaaaactg
      601 cagtggcctt attttgcgga aaccgagatg aacgacctga tcgtgttcct ttattcactc
      661 aattatctgg ataaacccgg ggatgccgca aagggagaga aaatcttctc tcagaaaaaa
      721 tgcatcgggt gccactttaa aaccgaggaa aacaaacaaa aactccttgg cgcattgaaa
      781 gcaatcaata ccacccaatt cgcggcggaa ttgtggaacc acacctccgc catggaagcg
      841 gccatggtga cccaaggagt tccctggccg gaaatgaccg gcgctcaatt gagggacgta
      901 ctggcgttcc tgcaaaaaca gtaaccaccg gaacaaaccg ctacccgcgt ttacaggtat
      961 cgattcgggc ggtcatcgcg gttgaggatg ggatgttgcg cgccggataa ggaaatacgg
     1021 gccgttcagg gagaatcgag atcctcttcc tctacgaccg cacgcttccg gacaagaaac
     1081 agctggtcgc cgatgtgcgg tttcaccccg tcttcgccta cgtggaaatc accgttcatg
     1141 atcgggacag caatccgcaa gagcagcgtg tacacgagca accccgtcgc ccagatcccg
     1201 atcgatacca gcatctcggt gcttgtcggg gaatattcgt agaaatcacc caggacatcc
     1261 gggacaaacc ccgggattac cagccccatc cccttctcga tatacacccc gatgaatatg
     1321 agaacgcagc cgatattgag cgtgacgaaa ttctccctgg tctgcggaat gaggaagaga
     1381 aagaacccag agatgttgaa gatcgtcgcg gtccagatgt acgaaaccag atccgagtgc
     1441 ccctccagtc cctggtagag atatttcacc ggagccaggt ggacggtgtc ggaataatac
     1501 tccttgaaga gctccgctcc cagcagaaac aggttgatga acatcgcgta cgcgatgagt
     1561 tcggcgattt tgaagatggc cttgtccttg atatcgaaat tcgtccactt ccggacgacc
     1621 tggaacagga ggatcatccc ggccggaccg gagcagaacg ccgaggccag aaaccggggg
     1681 gcaaggatcg atgcgttcca gaagggcctc gccgcaaggc cgttgtagag aaacgcggtc
     1741 acggtatgga ttgagatcgc cgccgggatc gaaaacatca ggaggggaaa gacgtatttc
     1801 atgttcggtt cgcgccgata gtaataacag tacagcatat agctcgcgat cgcgaagttc
     1861 aggacaaggt aagtgttcag taccacgacg tcccacgcca gaagcgactg tggaaagttc
     1921 aaaagcccga tcttcgggat caggtgccag aaccggtcgg gtcgcccgat gtcgaccgtg
     1981 acgaacaaca ggcacaccac catcgcggag acagccacaa gctcgccgag aatcgcgatt
     2041 tccttgatgg gcttccaatg gtagacatac gccgggatga cgagcaggat ggctgcagcc
     2101 gccaccccga cggtaaaagt gaaattggag atgtaaaaac cccaggagac ctgatcccgc
     2161 atcgcggtga cgatcagccc gttgtacatc tggttggcat acgcaaaaac cccgattgcg
     2221 atcagaacca ggagggcggt gagccaagcg tagtaaagct tgctcccttt catcaggagc
     2281 aggaaacacc ctcgcaggaa cgcccagacc ttctgcatca atcgcctccc ctgcggctca
     2341 cacggagtag aagtagtaga acttcggctg ggtgttcaaa tcctccttga ggatgaacac
     2401 acgcttgttc tcgatcacgt tgcggatctc gctggtcggg tcgagcaggt tcccgaactt
     2461 gcgggccccg acagggcaaa cctcgacgca tgcggggtat tccccgttcg gttccctggt
     2521 gcgctgcatg cagaagatgc acttctccac cacgccgacg ggccgcggcc ggttgccgag
     2581 ataatgcgtt tcgggattca tatccccggc aggcaggccg ggcgtcttcc agttgaaatg
     2641 cctcgccccg taagggcacg ccgccatgca ataccggcac ccgatgcacc agttgtagtc
     2701 cacgaccacg atcccatcga tctccttcca ggtggcctgg acgggacaca ccttggtgca
     2761 gggcgggttc tcgcactggt ggcactggac gggcatgtag aaatatcctt cctcggggac
     2821 cgtcgccgga ttgtaatatt gctcggaatg atccaggtcg acccctcgtt ccttctccag
     2881 gcggagcacc ttgatccaat gaatctgcgg gtcgcgggac tggttgttct cggtcacgca
     2941 cccgtaaacg cacctccggc acccgacgca acgggaaagg tccagtccgt acccgtacag
     3001 gacaccctcg atgggagacg tgtccttcac cctcaccggt ttcttgtact ttgcgctgta
     3061 ctccttttcg agccttgcca tgatcgctcg acgctcggac ttgccaagtt cgcggaaatt
     3121 cttctggaaa aacgactccc agacggaggc ggaggcgtct ttcgcaggga ggaccatggt
     3181 gcctatcccc aacgccaatc ccttcaacgc cttccggcgc gtcagccgga aaacgcgctc
     3241 gtcgtgt
//
LOCUS       DASSLD010000656         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1092202, whole genome shotgun sequence.
ACCESSION   DASSLD010000656 DASSLD010000000
VERSION     DASSLD010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092202"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..525
                     /locus_tag="VFK23_11865"
     CDS             40..525
                     /locus_tag="VFK23_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7319826.1"
                     /translation="MVTIRDLLQDDLIIEDIASTDKMGVLREFAGLLKARGRVADEEQ
                     LLGVLADREALGSTGIGDGVAIPHGKLSSIPETIVAFGRSRGGVDYQSLDAKPVFLFF
                     LLVTPVDRPGDHLKTLARISRILKNPALRADLKQAPHRQDIQRLIFEEDGKYPQPLAQ
                     K"
     gene            522..1406
                     /gene="hprK"
                     /locus_tag="VFK23_11870"
     CDS             522..1406
                     /gene="hprK"
                     /locus_tag="VFK23_11870"
                     /inference="COORDINATES: protein motif:HMM:NF019118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HET7319827.1"
                     /translation="MTITVKDLAAFSNVLAGSRGLGRRIACAQIAAPDLNAAAAWTGP
                     ASCVLHLRPGQIATLCSLAPRDLRSAVRDLLRQKPACIVISGGAVCTELLSRAETAGI
                     PVLKTGNVPRLARLLVERLSTRITVHGVLVQIFGLGTLIIGESAVGKSEAALDLVLRG
                     HKLTADDIVVIEKNDNEITGKPADLGENLLQIRGLGVVDVRALYGESATVNSSRIGLV
                     VELEEWRKGHQYSLVGLRERRHRLLGVNLPYLKLPVKPGRNMATLIEVAARNQILKNR
                     GVYTARELDKRLRKRLAG"
     gene            1403..>2063
                     /gene="rapZ"
                     /locus_tag="VFK23_11875"
     CDS             1403..>2063
                     /gene="rapZ"
                     /locus_tag="VFK23_11875"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET7319828.1"
                     /translation="MSRTRIIVVTGLSGSGRSAALKDFEDIGFYCVDNLPLALLPSFL
                     EFAGRSEEASRTAIGIDIREKGFTERFPSLFGELKARGFAIELIFLDASDHALVRRFS
                     ETRRPHPLARGETPLREGIRKEREALGQVKKLADRIIDTTDYTVHDLRQVIERHYAEA
                     DAGRPMVITLITFGYKFGVPFELDLLFDLRFLPNPHFVPGLRPLTGEDARIREYIMAG
                     HE"
BASE COUNT          391 a          636 c          667 g          369 t
ORIGIN      
        1 gggctcatcg agcccgccct ttaggatgga cgttccttca tggtcacgat tagagatctg
       61 ctgcaggacg acctgatcat cgaagatatc gcgtcgaccg acaagatggg ggtgctccgc
      121 gagttcgccg ggctgctgaa agcgcgcggg cgggtggcgg acgaagagca gcttctcggc
      181 gttctcgccg accgggaggc gctcggcagc acgggcatcg gcgacggggt ggccatcccc
      241 cacggcaaac tgagcagcat ccccgagacg atcgtggcct tcggcaggtc gcgcgggggg
      301 gttgattatc agtcgctgga cgcaaaaccg gtttttcttt tttttctgct cgtgacgccc
      361 gttgacaggc cgggcgacca tctgaagacc ctggcgcgta tctcgcgcat attgaaaaac
      421 cccgctctgc gggcggacct gaagcaggct cctcatcgac aggacatcca gaggcttata
      481 ttcgaagaag acggcaaata tccccagccg ctggcgcaga aatgacgatc accgtcaagg
      541 atctggcggc gttctcaaac gtgctggccg gcagccgggg cctcggcagg cggatagcat
      601 gcgcgcagat cgccgcgccc gacctgaatg cggccgccgc atggacggga ccggcgtcct
      661 gcgtgctgca cctgcgtccc gggcaaatcg ccactctgtg ctcgcttgct ccccgagacc
      721 tccggagcgc cgttcgcgac ctgctgcggc agaagcccgc atgcatcgtc atcagcggcg
      781 gggccgtgtg cacggaactt ctctcgcggg cggagaccgc cgggatcccc gtcctgaaga
      841 ccgggaacgt gcccaggctg gcccggctgc tcgtggagcg tctgtccacc cggatcacgg
      901 tgcacggcgt gctggtgcaa atcttcggcc tcggcacact catcatcggc gagagcgccg
      961 tcggcaagag cgaagcggcc ctcgacctcg tactccgcgg tcataagctg accgcggacg
     1021 atatagtcgt catcgagaag aacgacaacg aaatcaccgg aaaaccggct gatctcggag
     1081 agaacctgct gcagatccgg ggcctcgggg tcgtcgacgt ccgggcgctc tacggagaat
     1141 cggcgacggt gaactcctcg cggattggcc tcgtggtcga gctggaggaa tggcggaaag
     1201 gccatcaata cagtctggtc ggtctgcggg aacggcggca tcgtctcctc ggcgttaatc
     1261 tgccgtatct gaaattgccg gtcaagcccg gcaggaacat ggcaacgctc attgaggttg
     1321 cggcgaggaa ccagatactg aagaaccggg gcgtctatac cgcgcgggaa ctggacaaac
     1381 ggctccggaa gaggctcgcg ggatgagccg gacgcgcatc atcgtggtga cggggctttc
     1441 cggctccggc aggagcgccg cgctcaagga tttcgaggac atcggttttt actgcgtcga
     1501 taacctcccc ctggcgcttc ttccctcctt cctggagttt gccgggcggt cggaagaggc
     1561 ttcccgcacg gcgatcggca tcgatatcag ggaaaaagga ttcaccgagc gtttcccctc
     1621 gcttttcggc gagctcaagg cacgcggttt tgccatcgag ctcattttcc ttgatgcctc
     1681 cgaccatgcg ctcgtgaggc gtttcagcga aacgcggaga ccgcatccgc tggcgagggg
     1741 cgagacgccg ctccgggaag gcatcaggaa ggaacgggaa gcgctcggcc aggtgaagaa
     1801 gctcgcggac cggatcatcg acacgacgga ttacacggtc catgacctgc gccaagtcat
     1861 cgagcgccac tatgccgagg cggacgccgg caggcccatg gtgataaccc tcatcacctt
     1921 cggctacaag tttggagtcc ctttcgagct cgatctgctt ttcgacctgc gctttctgcc
     1981 gaaccctcat tttgtccccg gcctgcgccc gctgacgggc gaagacgcga gaatcaggga
     2041 atatatcatg gcagggcacg agg
//
LOCUS       DASSLD010000657         3568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_841911,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000657 DASSLD010000000
VERSION     DASSLD010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841911"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VFK23_11880"
     CDS             <1..1294
                     /locus_tag="VFK23_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7319829.1"
                     /translation="DFSEYFTEPDRARAGYLKVFREGSIRDYALDIRRGDGCATPALY
                     NASVYRDENGQVRGVFAVARDVTELKKAEKDRARLASAVESTADGVVITDPKTGEIQY
                     VNAAFEQITGYTKEEVIGRTLHILDSGRHDEEFYRVLRETLRREGVWRGRLINRKKDG
                     TLYFEDSTFSPVRDETGAIINYISVKRDITEKLRLESIAESVNTMDNIGYVFSGVRHE
                     IGNPINSAKMSLSVLQHKLDGASKSAIRDYVDRALGEIGRVEQLLKNLRNYNLYEKPE
                     LEVLSLKDFLGKFLHLVTEDFARKGIAIKHEYKDDSHQVVVDPRALQQVLLNLLTNSA
                     DALSGRSDPVIDISMSKKSGRVLLRVSDNGCGMTEKQQQDMFKPFYTSKPHGTGLGLV
                     IVKKMLTRMNGNIEITSSLNRGTTVTIYLPEGDPARNS"
     gene            1449..2807
                     /locus_tag="VFK23_11885"
     CDS             1449..2807
                     /locus_tag="VFK23_11885"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7319830.1"
                     /translation="MPLKGKKTLLVIDDEKVFCNNVRDYLESDRLEVLTANTGAEGLS
                     VCASKKVDVVLLDQKLPDGEGHALAPAILEKNEQAKIIFITAYPSFENAVAAVRLGAH
                     DYLSKPFDIDELKLTVENTLRTLDLERVEQVQIYARNKDREASVLVGNSAGFADVVRL
                     VESAGAMDAPVLITGETGTGKNLIAKTIHYRGPLAQAAFVSINCAALPENLIEAELFG
                     HEKGAFTGASGAKKGLFEIAEGGTIFLDEIGDMPLHLQSKLLSVLEDKALRRIGSTAV
                     TPVNVRIIAATNADLESALGKTFRKDLYYRLSVVRIHIPPLREHREDIPALCSYLLSQ
                     IAKGRQVEISDVEIEKLQEYDWPGNVRELRNILERALFLQKGTVLEPSRLIAKQVVAA
                     RPAPQTSAPAEVKSLAQVEQEFIQCTLSALSGNLTQTAKTLGISISTLKRKLKEYGLK
                     AR"
     gene            3165..3539
                     /locus_tag="VFK23_11890"
     CDS             3165..3539
                     /locus_tag="VFK23_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7319831.1"
                     /translation="MLRKNNVDVLLIDDEAPFLNDLAEGLRLNSQRLQVITATTAEKA
                     LEMLRTCKFDVVLTDLNMPGINGYQVLREVEQRHPGTPVIIMSAYARNAVADRLEGLR
                     FTGYLEKPLDLREVTEAILDAG"
BASE COUNT          821 a         1067 c         1036 g          644 t
ORIGIN      
        1 cgacttctcc gagtatttca ctgagccgga cagggcgcga gccgggtacc tgaaggtctt
       61 ccgcgaaggc tcgatcaggg actacgccct ggatatccgg cgcggcgacg gctgcgcgac
      121 gccggctctg tacaacgcgt cggtataccg cgacgagaac ggacaggtgc gcggcgtctt
      181 cgcggtggcc cgcgacgtca ccgaactgaa gaaggccgaa aaggaccggg cgcggctcgc
      241 atcggcggtg gaatccacgg ccgacggcgt ggtcatcacc gacccgaaga caggcgagat
      301 ccagtacgtg aacgctgcat tcgagcagat aacggggtat actaaggaag aggtgatcgg
      361 gcgcaccctc catatcctcg acagcggcag gcacgatgaa gagttctacc gtgtgctccg
      421 ggagacgctc aggcgcgaag gcgtgtggcg ggggcggctc atcaacagga agaaggacgg
      481 caccctctat tttgaggact ctacgttctc gcccgtgcgg gacgagacgg gggcgatcat
      541 caattacatc tcagtgaagc gcgacatcac ggagaagctc cggctcgagt ccatcgccga
      601 gtcggtgaac accatggaca acatcggcta cgtcttctcc ggggtccggc acgagatcgg
      661 gaacccgatc aactccgcca agatgagcct ctccgtgctg caacacaagc tggatggcgc
      721 gtcgaagtcc gccatccggg actacgtgga ccgggccctg ggcgagatcg gcagagtgga
      781 acagctcctg aaaaatctca ggaactacaa cctttacgaa aaaccggagc tcgaggtcct
      841 gagcctcaag gacttcctcg gaaagttcct gcaccttgtc accgaggatt tcgcaaggaa
      901 agggatcgcc atcaagcacg agtacaagga cgactctcat caggtcgtgg tggacccgcg
      961 ggcgctccag caggttctcc tgaacctcct gaccaactcc gcggacgccc tctccgggag
     1021 gagcgaccct gtgatcgaca tctcgatgtc aaaaaagtcc gggagggtcc tccttcgcgt
     1081 gtcggacaac ggctgcggca tgacggaaaa gcagcagcag gacatgttca agccctttta
     1141 tacttcgaaa ccccatggaa cggggctcgg actggtgatc gtcaaaaaaa tgctgaccag
     1201 gatgaacggg aacatcgaga tcacgagctc gctcaaccgg ggcacgaccg taaccattta
     1261 tctgccggag ggggatccag cccgcaactc gtagctcatc gttgagggtt catctgcccg
     1321 ggctgccctt cgcgctgcga aacttgtcct cgaatgtctc gatcggggat ggattcccga
     1381 tagaatcatt cgggaatgac ggttcggttt tctatagtcc ataccaagcc ttaaggagaa
     1441 gcaacatcat gccgcttaag ggaaaaaaga cgcttctggt gatcgacgac gaaaaggtgt
     1501 tctgcaacaa cgtgcgcgac tacctcgaga gcgaccgcct ggaggtgctt accgcgaata
     1561 cgggcgcgga agggctctcc gtatgtgcga gcaagaaggt cgacgtcgtt ctccttgacc
     1621 agaagctccc cgacggagag ggacatgccc ttgcccccgc catcctggaa aagaacgaac
     1681 aggccaagat catcttcatc accgcctatc cgagctttga aaacgccgtg gcggccgtgc
     1741 gccttggtgc tcacgactac ctgtccaagc ccttcgacat cgacgaactc aagctcacgg
     1801 tcgagaacac gctccggacc ctcgacctgg agcgggtcga gcaggtccag atctacgccc
     1861 gcaacaagga ccgcgaggca tcggtcctcg tgggaaacag cgccggcttc gccgacgtgg
     1921 tcaggctcgt cgagtcggcc ggggccatgg acgcgccggt cctcatcacc ggagaaaccg
     1981 gcacgggcaa gaacctcatc gccaagacca tccattaccg cggccccctg gctcaggcgg
     2041 ctttcgtcag catcaactgc gccgccctcc cggaaaacct gatcgaggcg gagctgttcg
     2101 gccatgaaaa gggggccttt acgggtgcat caggggccaa gaagggcctg ttcgagatcg
     2161 ccgaaggcgg cacgatcttc ctggacgaga tcggcgacat gcctctccat ctccagtcga
     2221 aactcctctc tgtgctcgaa gacaaggcac tccgcaggat cggcagcacc gcagtcactc
     2281 cggtcaacgt gcgcatcatt gcggcgacga acgccgacct agaaagcgcc ctgggcaaga
     2341 ccttccgcaa ggacctgtac taccgcctga gcgtggtccg catccatatt ccgccgctca
     2401 gggagcaccg cgaggacatc cccgcgctct gcagctacct gctctcgcag attgcgaagg
     2461 gccggcaagt cgagatctcc gatgtcgaga tcgaaaaact ccaggaatac gactggccgg
     2521 gaaacgtccg ggagctgcgc aacatcctgg agcgggcgct tttccttcag aagggcacgg
     2581 tcctcgaacc gtcccggctg atcgcgaagc aggtggtcgc ggccaggccg gctccgcaga
     2641 cgtccgcacc ggcggaggtc aaatccctcg cgcaggtgga acaggaattc attcaatgca
     2701 ccctatcggc gctttcgggc aacctgacgc agaccgccaa gaccctgggt atttccatct
     2761 cgacgctcaa gagaaaactg aaggaatacg gcctgaaagc ccgttagagc cgtggtcgtc
     2821 agcgaagagg acacgagtct ctagcagttt gctgaaaaag tctccgttca cccttcaata
     2881 ggctcagggc gaacggaagg agtgctgtaa taataagctt ttatccgttc atggtgagct
     2941 gttgaaccac atcaaagact ttttcagcta cctgttaggc ctccggcgta atggattttg
     3001 cgactcttga gggggctaaa ataagcgacc cgctcaaaat gagcaactca gaataacctc
     3061 atccagctta aaaaatccct tctacatctc tgcaacttgt tgttttttca gaaccattcc
     3121 cttccaaaaa cttttggcat agcctttgct ctaaagaata catcatgttg agaaaaaaca
     3181 atgttgatgt actcttaatc gatgatgaag cgccttttct caatgacctc gctgagggcc
     3241 tgcgcctgaa ttcacagagg ctgcaggtca tcactgcgac caccgcggaa aaggccctcg
     3301 agatgctccg gacgtgcaag ttcgacgtcg tgctgacgga cctcaacatg ccagggataa
     3361 acggatacca ggtgctgcgg gaagttgagc aaagacaccc cggcacgccc gtcatcatca
     3421 tgtcggccta tgcacgaaac gccgtggcag accggctgga aggcctgcgc tttaccggtt
     3481 atctcgagaa gcccctcgat ctccgcgagg tcactgaggc gattctggac gcgggctgac
     3541 cccagaggcg aggccgggcc gggcggtg
//
LOCUS       DASSLD010000658         3275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1222591, whole genome shotgun sequence.
ACCESSION   DASSLD010000658 DASSLD010000000
VERSION     DASSLD010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222591"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VFK23_11895"
     CDS             complement(<1..721)
                     /locus_tag="VFK23_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005983938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET7319832.1"
                     /translation="MDKILRIDVGAEGGPKARETPIGEYAGLGGRAMTSAIVSKEVPP
                     LCHPLGAENKLVIAPGLLSGTAGAQSGRISVGGKSPLTGGIKEANSGGQASQVLAKLG
                     YGAIVLEGKPTGDDLYKVFINKDGVKITKDNSLKMLTIYDLVEKMKKEYGDKMCCISI
                     GPCGEMKMGAATVAFTDMELRPTRHAGRGGLGAVMGAKNIKVIVLDSTGMPVRPPKDP
                     VKFQDANKRFVEGLKRHAVTGQ"
     gene            complement(1071..2237)
                     /locus_tag="VFK23_11900"
     CDS             complement(1071..2237)
                     /locus_tag="VFK23_11900"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET7319833.1"
                     /translation="MAIKEQVYGFYIPTVTLMGIGAHKELGSRIKTLGAKKPLIVTDK
                     GITKSGLTQQIVDLVRKDAGLEPVVYDETIPNPTDKNVHDGVDVYKKNGCDMIITLGG
                     GSSHDCGKGIGIVAANGGSIKDYEGIDKSTKSMPPFIAINTTAGTASEMTRFCIITDT
                     SRKVKMAIVDWRVTPTIAINDPLLMMGMPPALTAATGMDALTHAVEAYVSTIATPVTD
                     ACALKAIELVAKNLRAAVANGGDMVARDNMAYAEYLAGMAFNNASLGHVHAMAHQLGG
                     FYNLPHGVCNAILLPHVERFNMIAKMDRFGDIAKAMGENIEGLSPRVAAEKAIEAIKT
                     LSRDIGIPSGLTELGVKEKDLRIMAENAQKDACGLTNPRCPSLEDVINIYKAAA"
     gene            complement(2403..>3275)
                     /locus_tag="VFK23_11905"
     CDS             complement(2403..>3275)
                     /locus_tag="VFK23_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319834.1"
                     /translation="GGFLLDLGGRDTFVGLTGSQGAGFFGLGLLYHGDGDAGFSASRY
                     AQGTGLMGIGILASRKGDNKYVCSYAGQGFGLPGGAGILIDEEGNDYYQLGGREPDFR
                     DPQKATQSFGQGFGQGVRSEKNVNAAPGGIGMLIDERGNDTYLADYFAQGASYYYGLG
                     ILDDRAGDDQYFAGRYAQGAGIHSSVGVFVDSRGNDFYYLSYGVGQGMGHDYGVGFLE
                     DDEGENRYQAGTLVQGAATIGSIGILIDPKGPDKLSYQNSGQAYAETDNSMGIVIRKG
                     GGRDTSVKIGVKKE"
BASE COUNT          603 a         1028 c          869 g          775 t
ORIGIN      
        1 cctgtccggt gactgcatgc ctcttgagac cttcgacgaa ccgcttgttg gcgtcctgga
       61 actttaccgg gtccttcgga ggccgcaccg gcatgccggt ggaatcgagt acgataacct
      121 tgatgttctt ggcgcccatg accgcaccca gacctccgcg tcctgcgtgg cgcgtcggcc
      181 ggagctccat gtcggtgaac gcaacggtgg cggcgcccat cttcatctct ccgcaaggtc
      241 cgatcgagat gcagcacatc ttgtcgccgt attccttctt catcttctcg accagatcgt
      301 aaatcgtgag catcttgagg ctgttgtcct tcgtgatctt gaccccgtcc ttgttgatga
      361 agaccttgta caggtcgtcg cccgtcggtt tcccttcgag cacgatggcg ccgtagccga
      421 gctttgcaag gacctgggag gcctgtcccc cggagttcgc ctccttgatg cccccggtaa
      481 gcgggctctt gccgccaacg gagatccggc ccgactgggc gcccgcggtc ccgctcaaaa
      541 gccccggcgc gatcaccagt ttgttctccg cgcccaatgg atggcagagg ggcggcactt
      601 ccttcgagac gatagccgag gtcatggccc tccccccgag ccccgcgtac tcgccgatcg
      661 gcgtctccct ggccttcgga ccgccctcgg cccccacatc gattcgcaat atcttgtcca
      721 tctgttcctc ctgtctcctc tgcggcgaga ggtgaaaaat aaggggttta aaaaatataa
      781 ttccttatga gagccctaag atccacttcc gtcaccctga gcaacgcgaa gggtctggca
      841 actgccggaa aacaagatac ttcgaggagt ttctcctgag caattagatt ccagattctt
      901 cggcttcgcc tcagaatgac aaaaccggac cggttcgtca tccatgcaac agcctctttg
      961 acaatttcaa ttattcatga agtcgtgcag cggtcgagac taaataatta tatcagaacc
     1021 cgccccggcc atgcggggcg ggtcaatcgg tttgaatatt tttctgctac ttaagccgca
     1081 gccttgtaaa tattgatcac gtcctccaga gacgggcacc gtgggttcgt cagaccgcag
     1141 gcgtccttct gggcgttctc ggccataatc cggaggtctt tttccttcac acccagttcg
     1201 gtaaggcctg acgggatccc gatgtccctg gaaagcgtct tgatggcctc aatcgccttt
     1261 tcagcagcaa ctctgggtga caggccctca atgttctcgc ccatggcctt cgcgatgtcc
     1321 ccaaagcggt ccatcttggc gatcatgttg aaccgctcca catgcggcag caggattgcg
     1381 ttgcacaccc cgtggggcag gttgtagaac ccgccgagtt ggtgcgccat tgcatgcacg
     1441 tgacccaggc tggcgttgtt gaaggccatg cccgcgaggt actcggcgta agccatgttg
     1501 tcccgggcga ccatgtcgcc gccgtttgcc accgcggcgc gcaagttttt cgctacgagt
     1561 tcaatggcct tgagcgcgca ggcgtcagtg accggtgtgg cgatggttga cacataggct
     1621 tccacagcgt gggtcagtgc atccattccc gtggctgccg tcagggccgg cggcatgccc
     1681 atcatgagca gcgggtcgtt gatcgcgatc gtgggtgtca ccctccagtc aacgatggcc
     1741 atcttcacct tgcggctcgt gtcggtgatg atgcagaacc gggtcatctc gctggcagta
     1801 cccgccgtgg tgttgatggc gataaagggc ggcattgact tggtggactt gtcgatgccc
     1861 tcgtagtcct tgatgctgcc gccgtttgcg gccacgatgc cgataccttt gccgcagtcg
     1921 tgggagctgc ccccgccgag tgtaattatc atatcgcagc cgttcttctt gtagacgtcc
     1981 accccgtcgt ggacattctt gtccgtgggg ttcgggatcg tctcgtcata gaccaccggc
     2041 tccaggcccg cgtccttcct gaccagatcg acgatctgct gggtcagacc tgacttggta
     2101 atccccttgt ccgtaacaat aaggggcttc ttcgccccca gtgtcttgat cctgctcccc
     2161 agttccttat gcgctccgat ccccatcagg gtaaccgtgg ggatgtaaaa tccgtaaacc
     2221 tgttccttga ttgccatata catcacctcc ccttccactt cttgcgatgg ctcactgcgt
     2281 tcaaagagaa gaaatattgc gaaaatattt tttttgcttc tcttaattta aagcttagga
     2341 gtgctctgac ggtttgtcaa tagagaccaa agtaggagtg ctggaggaat cgaggcggta
     2401 atctactctt tcttaacccc gatctttacg ctggtatcgc ggcctccgcc ttttcggatc
     2461 acgataccca tactgttgtc tgtttccgca tatgcctgac cgctgttttg ataagacagc
     2521 ttgtccggtc ctttcgggtc tatcagaatg ccgatactgc cgatcgtcgc tgcgccctgc
     2581 accagggtcc ccgcctggta gcggttctcc ccttcgtcat cctctaaaaa accgacgccg
     2641 taatcatgcc ccatgccctg ccccacaccg taggacaaat agtagaaatc gttccctcgg
     2701 ctgtcgacga aaaccccgac agaggagtga atccctgcgc cctgggcata gcggcctgca
     2761 aagtattgat catcgccggc cctgtcgtcc agaatgccca ggccatagta ataggacgcg
     2821 ccctgggcga aatagtccgc gagataggtg tcgtttcccc gttcgtcgat aagcatgccg
     2881 atgccgcccg gcgcggcatt tacatttttc tccgaacgca ctccctgtcc gaacccctgg
     2941 ccgaagctct gggtggcctt ctggggatcg cggaaatccg gctcccggcc tcccaactgg
     3001 taataatcgt tcccttcttc atcgatcagg atgcccgccc ctccggggag accgaaaccc
     3061 tgtccggcgt aggagcagac atacttgttg tctcccttcc ggcttgccag gatcccgatg
     3121 cccatcagac ccgtaccctg agcataccgg ctcgcgctga atcctgcgtc tccgtcgccg
     3181 tgatagagga gtcccagccc gaagaaaccc gctccctggc ttccggtgag tcccacgaac
     3241 gtgtctctgc cgccgaggtc caggaggaaa ccccc
//
LOCUS       DASSLD010000659         3833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_309941,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000659 DASSLD010000000
VERSION     DASSLD010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309941"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VFK23_11910"
     CDS             <1..577
                     /locus_tag="VFK23_11910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HET7319835.1"
                     /translation="DGDPSGGTVYAVEDDSVRRIISAQGRAAQMNRGAEAASGDILLF
                     LHADTRLPGNALPLILESMGNGRFVAGAFDLGFDTKRSIFRVTESYVFLRTRLTRVPF
                     GDQAIFIRSDYFRHLGGYREVPIMEDVDLMKRIRRKGGRIRIIPEKVSTSPRRYEREG
                     VLACTFRNWALQMLFALGVSPERLAKRYRPF"
     gene            651..1325
                     /locus_tag="VFK23_11915"
     CDS             651..1325
                     /locus_tag="VFK23_11915"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET7319836.1"
                     /translation="MKKVVLASGLTALFEDKGSFLDRADIEVFTAATNDEVLRIHKDE
                     HADLIVTTIDLPGARIEDLFSAIRNDDDLRKVSTIIICKDTLTHRDRCKQCRANAVFP
                     MPVDTAVLALKAQQFLNVLPRKLYRAPLAVGIQGRFRDRPLPFRTENISAGGMLIRTE
                     EPLAKGDGIFFSFFLPDGTHISGYGEIARVEQSETGTYHYGIKFTNVSPADSSAIEEA
                     IKKIRS"
     gene            1430..2149
                     /locus_tag="VFK23_11920"
     CDS             1430..2149
                     /locus_tag="VFK23_11920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HET7319837.1"
                     /translation="MTAHQKERSRGFNNIMPSTSIIILFAKIPVKGRVKTRLARDLEE
                     DLALRFSESMLLDTIDMLKDCGFPFRICIAPPGAVETARARLGGGYAFMPQTGDDLGE
                     RMEQAFVRVFTEGFREAVLIGSDIPGLSAGIVREAFASLSEHGAVIGPANDGGYYLIG
                     FTRGAFWPEIFHSMPWSTPAVFGETLGRLASASRRVHVLPECIDVDTSEDLITLLQQK
                     DRPASPRTLALLKRQGRGLAK"
     gene            2155..2919
                     /locus_tag="VFK23_11925"
     CDS             2155..2919
                     /locus_tag="VFK23_11925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET7319838.1"
                     /translation="MYTKPYAIIAALVIIFTLEGVFPHRKGRRQRIPHAVPHVLTAIL
                     NGVLTKLLLAGITLQAASWTEARTAGIMNLFTLPGPVRTALVFVLFDIWMYLWHMANH
                     RSAFLWRFHRAHHADTEMDTTTALRFHPGELILSAFARLPVIVIIGMSYSELIIFEIL
                     LNLSTLFHHSNLGIPEKWDRLLRVVIVTPDMHRVHHSVERFETDSNFTSLLSVWDRLA
                     RTFRMREDTRTVVFGLPVFREARYQRLKGFLITPFV"
     gene            2974..3666
                     /locus_tag="VFK23_11930"
     CDS             2974..3666
                     /locus_tag="VFK23_11930"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET7319839.1"
                     /translation="MSGRNKKILLLVFVILTVITLRSSPLGSALTFENLQRNRQALLS
                     FVSAHYVLSVVSFISVYVIVAALSIPGAVILTLAGGFLFGTAAGVLYVDAGATAGAVC
                     AFLSARYLLGDRLQQKYGGQFAKFNGEMVRNGVNYLLTLRLIAVFPFFLINFLAGLTK
                     VPLKTFLWTTAVGIIPGTAVFAYAGQQLGSINSPADILSKKVIAALAALGFITLAPTV
                     WKRIRKAKTQYP"
BASE COUNT          783 a         1163 c         1080 g          807 t
ORIGIN      
        1 cgacggggac ccctcgggcg gcacggtata cgccgtcgag gacgactccg tccggagaat
       61 catttcggcg cagggccggg cagcgcagat gaaccgcggc gccgaggccg cctcgggtga
      121 catcctcctc tttctccatg cagacacccg cctccccggg aacgcgcttc cgctgatact
      181 cgagtccatg ggcaatggac gctttgtcgc cggcgcattc gatctcgggt tcgacacgaa
      241 acgaagcatc ttccgcgtca cagagagcta cgtttttctc cgcacccgcc tcaccagggt
      301 ccctttcgga gaccaggcca tttttatccg cagcgattat ttccgtcatc tcggcgggta
      361 ccgggaggtc cccatcatgg aagacgtgga cctgatgaag agaatcagga ggaagggagg
      421 ccggatccgc atcattcccg aaaaagtgtc gacttccccg cgtcggtacg agcgggaagg
      481 ggtcctcgcc tgcacattca ggaattgggc gctgcagatg cttttcgcct tgggcgtatc
      541 tccggagcga ctcgcgaagc gatatcgacc cttctaacgg gccaggcgcg gtaatggctt
      601 gcattgggct gcgtgctatg gtactgtaat cccaaaggac gatgccgggt atgaaaaagg
      661 tggtgctggc gagcggcttg acggctctct tcgaggacaa gggcagcttt ctcgatcgcg
      721 ctgacataga ggtattcacg gcggccacga acgacgaagt gctgagaatc cataaggacg
      781 agcacgccga tctcatcgtc acgacgatcg atctgcccgg agccagaatc gaagatctct
      841 tcagcgccat cagaaatgac gatgacctcc gaaaggtttc caccattatc atctgtaagg
      901 acaccctcac ccaccgggat cgctgcaagc aatgccgcgc caacgccgtt ttcccgatgc
      961 ccgtcgatac agcagtgctg gccctcaagg cgcagcagtt tctcaatgtc ctgccgagga
     1021 aactctaccg ggcgccgctc gctgtcggca ttcaaggcag attcagggac cggccgctgc
     1081 cgttccggac ggagaatatc agcgcgggag ggatgctcat caggaccgag gaaccccttg
     1141 caaagggcga cggtattttt ttctccttct ttctccccga cggtacgcac atcagcggat
     1201 acggcgaaat agctagggtc gaacaatcgg aaacgggcac ctatcactac ggcatcaagt
     1261 tcaccaacgt ctccccggcc gactcgtcgg caatcgaaga agcgatcaaa aaaatccgat
     1321 cataacctcc gacatcccca cgcagacttc ctcgccattc cagaaacggc gcacaggccg
     1381 gtcgtcactt gaagtgcggg actatttgtg caatactgat gatcagatca tgacggcgca
     1441 ccagaaggaa cgcagccgcg ggtttaacaa tatcatgccg agcacatcga ttatcatcct
     1501 tttcgcgaag atcccggtta aggggcgggt caagacgcgc ctggcgcggg acctggagga
     1561 agatcttgcc ctccgtttct ccgagagcat gctcctggac acgatcgaca tgctgaaaga
     1621 ctgcggcttt ccgttccgaa tctgcatcgc tcctccgggc gcggtcgaga cggcaagggc
     1681 gcggctgggg ggcggttacg cgttcatgcc gcagaccgga gacgacctgg gagagcggat
     1741 ggagcaggcc tttgtccgtg tatttaccga aggcttccgc gaggcggtcc tgatcgggag
     1801 cgacataccg gggctttccg ccgggatcgt gcgcgaggcg ttcgcatccc tcagcgaaca
     1861 tggcgcggtc atcggtcccg caaacgacgg cggttactac ctgatcggat ttaccagggg
     1921 ggcgttctgg cccgaaattt ttcattccat gccctggagc acccctgctg tattcggaga
     1981 gaccctgggg cggctcgcgt cggcatcccg tcgtgttcac gtgctgccgg aatgcatcga
     2041 cgttgacaca agtgaggact tgataacatt gctgcagcag aaagaccggc ccgcttcccc
     2101 gcgcaccctg gccctgctca agcggcaggg tcgcggtctt gcgaaataga ggcgatgtac
     2161 acgaaacctt atgcaatcat cgcagcactc gtcatcatct ttacgctcga gggcgtcttt
     2221 ccccacagga agggccgtcg gcagaggata ccgcatgcag ttccccacgt cctgacggcg
     2281 atcctgaacg gggtcctgac gaaactgctt cttgccggca tcacgctgca ggcggcctcc
     2341 tggactgaag cacggacggc ggggatcatg aacctgttca ctctccccgg cccggtccgg
     2401 accgccctgg tattcgttct gttcgatatc tggatgtacc tctggcacat ggccaatcat
     2461 cggagcgcgt ttctgtggcg cttccaccgt gcccaccacg ccgacacgga aatggacacg
     2521 acgacggcgc tgcgctttca tccgggcgaa ctaatccttt cggcattcgc gcggcttccc
     2581 gtcatcgtca ttatcggaat gagttattcc gaactgatta tcttcgagat cctgctgaac
     2641 ctctcgacac tgttccatca cagcaacctc ggcatcccgg agaagtggga ccggttgctg
     2701 cgcgtcgtga tcgtaacgcc cgacatgcac cgggtccacc actccgtcga gcggttcgag
     2761 accgattcta atttcacgag cctgctttcc gtctgggacc gtctggcccg gaccttccgt
     2821 atgcgcgaag acacgcgcac ggtcgtcttc ggccttcccg tgttcaggga ggcccgctat
     2881 cagcggctca agggcttttt gatcacgccg ttcgtgtaac agagaaatat cgccacagat
     2941 acatttataa aaagagcggc agacggtgcg accatgtccg gacggaacaa aaaaattctc
     3001 ctgctcgttt tcgtcatact cacggtcata accctgcggt cttctccgct cggctcggcc
     3061 cttacctttg aaaatctgca gcggaaccgg caggcgctcc tctccttcgt gagtgcgcat
     3121 tatgtcctgt ccgtggtgtc cttcatttcc gtatatgtca tcgtggcggc cctctccatt
     3181 ccgggcgcgg tcatcctgac gcttgccggg ggtttcctct tcggcacggc tgcgggcgtc
     3241 ctatacgtcg atgcgggcgc aacagcgggg gcggtgtgcg cgttcctttc ggcccggtac
     3301 ctgctggggg accggctgca gcagaagtac gggggccagt tcgcgaagtt caacggcgag
     3361 atggttcgca acggcgtaaa ctatctgctc acactgcgcc tgatcgcggt ctttcccttt
     3421 tttctcatta attttctggc cggcctcacg aaggtcccgc tcaagacctt tctctggacg
     3481 acggcggtcg gcatcatccc cggcacggcc gtatttgcct atgccggcca gcagctcggg
     3541 tccatcaatt ccccggcgga catcctttcg aagaaggtca ttgccgccct ggccgcgctc
     3601 gggtttatta cgctggcgcc gacagtctgg aagcgcatcc gaaaagcaaa aacgcagtat
     3661 ccataacgat attcccaagg gttaagcacc aaataacaag ctccaaatta caaataagcc
     3721 ccaagcacca atgatcaaat tcaaaacgtt tcgggaattg gaatgccgga atttatttga
     3781 aatttgtttt ttgggatttg aaatttatac ctgcaaggag gtcccatgga caa
//
LOCUS       DASSLD010000660         6647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_499031,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000660 DASSLD010000000
VERSION     DASSLD010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499031"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..2996)
                     /gene="ileS"
                     /locus_tag="VFK23_11935"
     CDS             complement(210..2996)
                     /gene="ileS"
                     /locus_tag="VFK23_11935"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET7319840.1"
                     /translation="MDYKTTLNLPRTDFPMKANLKDLEPRVIEKWRREKIHDRLQEQT
                     AGRGTYILHDGPPYANGHIHIGHALNKILKDIIVKFKTMEGFRSPYVPGWDCHGLPIE
                     HQVLKSLGPKKEGMSQIEIRRLCREYAEKFINVQREEFKRLGVFGDWDHPYLTMDFSY
                     EAAIVRELGKFVAAGSVYKGKKPVYWCGFDETALAEAEVEYADHESPSVYVRFELPDA
                     GKAIPALNGKAAAIAIWTTTPWTLVANMAVALHPEFDYVAVETGGSALILAEGLLKQS
                     MEKFGFREYRVLDKFKGRKLEGLKARHPFITPERESLVILGDHVTLEAGTGAVHTAPG
                     HGQEDYEAGLKYGLEVYAPVDSKGRFTKEADGFAGQQVFKANKGIIELLRSKGALLAE
                     EKILHSYPHCWRCKNPVIFRATEQWFISMKTNELLKKTVENIHKVSWVPSWGKDRILG
                     MMENRPDWCISRQRAWGVPIVAFTCKQCGELLLDHSVVDHVAGIVEREGADAWFVRTP
                     AELLPPGTQCKKCGGSNLEKEMDILDVWFDSGVSHAAVLRQRKELSWPADLYLEGSDQ
                     HRGWFQSSLLTSVATTGRAPYNSVLTHGFTVDGSGKKMSKSAGNVVAPQEVIDKYGAE
                     VLRLWVSAADYRDDIRISQEILTHLAEAYRRIRNTSRYLLGNLADFDPGTDRVSDKEL
                     LEIDRWALHRLQRLIQRVRKGCDDFEFHIVFHSIHNFCAVDMSAFYLDILKDRLYTAK
                     PKSVGRRSGQTAMYEVLSAMVRLMAPVLSFTADEVWGFINDRAKPESVFLAPFPQAEE
                     RYIDEGLDERWDRIIAVRGETAKVLEALRRDKKIGHSLDAGVTLYADPGLREFLAGYR
                     KDLAFIFIVSSVEIAPESAAPEGAYASDAVKGLRIAAGPAKGAKCGRCWMYSETVGKV
                     PDHPAICGRCAGNL"
     gene            complement(3075..5534)
                     /locus_tag="VFK23_11940"
     CDS             complement(3075..5534)
                     /locus_tag="VFK23_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET7319841.1"
                     /translation="MVKLLETLKKITLLAWEKFKAFWFGVAVPFVNGLTPKKALAILG
                     LLFVAGVLTLALMFYILSFGLPTIESLKDYKPSAGTMILAKDGRVLGQVRIEKGIYVP
                     LHRIPKQMVNALLATEDPRYYQHKGIDYRGILRAALKDIFSLRLKQGGSTITQQLTKV
                     IFLSPERKLTRKIKEIILARRIEKELTKEDILELYLNKIYFGHGAYGVQMAAKTYFGK
                     NIWEVNLAEAALLAGLPQSPTGYSPYGDIDLTKMRQMHVLHRMAEEGYITEAQAKQTY
                     DQPLNLENLRSQEDIAPHVVDYVRMYVEKKYGTDKLYQGGLKVSTSIDFDLQRAAVSA
                     LHEGLRALDKRQGFRGRVGFKELKGNQVKSGSFRVMVKPGEGFNAQVLEVGDTSVTVR
                     GRGLLGVIARDDMAWALARQRRKEGEPEFRKPSEIIKPGDIIKVRFKDFDRKKQLASF
                     ILDQTPLVEGAVVSIEPYTGYIRALVGGYDFVEGGFNHAMDARRQPGSSFKPFVYGAA
                     LENGFTPASILMDLPIIHEKSEYDKKGWKPENYDDRFLGPMRLRTALALSRNAVTVAL
                     LEKIGLDKTIDFARKAGIRSQISHDYTTALGSSAVTPLELTSAYATFASQGVRSEPIL
                     ITSIADSRGQVLESYEPQPVEALNPTTAYLVDSLLKSVVEEGTGRAARVLGKPVAGKT
                     GTTNKYVDAWFVGFTPSIVTGVWVGYDNPRASLGNKETGARAALPIWISVMSNALADK
                     PAEDFIPPDDDVVTVKIDPETGLLARDGQENAVTDVFRRGTEPIQYSDTVRRSTGQLY
                     NIDQGDAGETPKKYDPEEITD"
     gene            complement(5737..6471)
                     /locus_tag="VFK23_11945"
     CDS             complement(5737..6471)
                     /locus_tag="VFK23_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET7319842.1"
                     /translation="MDWLTTPEAWIALVTLTALEIVLGVDNIIFISILVGKLPERQRS
                     KARVLGLGLAMLTRIGLLLSIVWVMKLTTPLFTVFAQEISGRDLILIVGGLFLLWKST
                     LEIHDSLEGAEEEITGKAEAAFAVTLAQIAVLDIIFSLDSVITAVGLAKEVAVMIIAV
                     VLAVIVMMFSSGAIGSFVDRHPTIKMLALSFLVLIGVTLIAEGLDLHVPKGYIYFAMA
                     FSVIVEMLNMRLRKKTSPVKLHKPLE"
BASE COUNT         1253 a         2038 c         1928 g         1428 t
ORIGIN      
        1 caacgaggtg tttcgtgaac tgatcgaggg ccagcaccat cccgatgatc gaaccgaccc
       61 aaatgtattt atgtcgcaaa ctgctcccct ttttgaaatg aaatgacgac ggggtgacac
      121 ggagacgggg agagcgggag aaaaagcgat gtgccacatc gccgtttcgc cccgtcaccc
      181 actcgccgcg tcgcccactc gccgattcgt tacaggttcc ccgcgcatct tccgcagatc
      241 gccgggtggt ccggcacctt gcccacggtc tcgctgtaca tccagcagcg tccgcacttg
      301 gcgccctttg ccggaccggc agcgatcctg agtcccttga ccgcgtcgct cgcgtacgct
      361 ccctcgggcg ctgccgattc cggcgcgatc tcgacggagg agacgatgaa gatgaaggcc
      421 agatcttttc gataccccgc caggaattca cgaagccctg gatcggcgta gagcgttacg
      481 cccgcatcga gggaatggcc gatcttcttg tccctccgca gggcctcgag caccttggcc
      541 gtctctcccc gcaccgctat gatcctgtcc caccgctcgt caaggccttc gtcgatatac
      601 cgctcttcgg cctgcgggaa cggggcaagg aagacgctct cgggcttcgc acgatcattt
      661 ataaagcccc agacctcgtc ggcggtgaac gaaagcaccg gcgccatgag gcgtaccatt
      721 gccgagagga cctcatacat ggctgtctgt ccggacctgc gtcctaccga cttcggtttc
      781 gccgtataca gccggtcctt caggatgtcg aggtagaagg cgctcatgtc cacggcacag
      841 aaattgtgga tggaatggaa cacgatatga aactcgaaat cgtcgcagcc cttccggacg
      901 cgctggatca gcctctgcaa gcggtgaagc gcccagcggt cgatctcgag gagttccttg
      961 tccgaaacgc ggtcggtccc ggggtcgaag tccgcgaggt tgccgaggag atacctgctc
     1021 gtattcctga tcctgcggta cgcctctgcc aggtgggtca ggatctcctg ggagattctg
     1081 atgtcgtccc ggtagtcggc agccgacacc cagagcctca gcacctcggc gccgtacttg
     1141 tctatcactt cctgcggcgc caccacgttt cccgccgact tgctcatctt cttgccggag
     1201 ccgtcgacgg taaagccgtg ggtcaggacc gagttgtacg gggcccgccc cgtcgtcgcg
     1261 accgaggtga ggagcgacga ctggaaccac ccgcggtgct ggtcgctgcc ttcgaggtag
     1321 aggtccgccg gccaggagag ttcctttcgc tggcggagaa cggcggcgtg gctcaccccc
     1381 gaatcgaacc agacgtccag gatgtccatc tccttttcga gattgctgcc tccgcacttt
     1441 ttgcattggg tgcccggcgg caataactcg gcgggagtgc gtacaaacca cgcgtcggcc
     1501 ccctcgcgct cgacgatccc cgccacgtgg tctacgacgc tgtgatccag caggagttcg
     1561 ccgcactgtt tgcaggtgaa ggcgacgatc ggcacgcccc atgcccgctg gcgggaaata
     1621 caccagtccg gacggttctc catcatgccg aggatgcggt ccttgcccca ggagggtacc
     1681 caggagactt tgtggatgtt ctcgaccgtc ttcttcagga gctcgttcgt cttcatcgag
     1741 atgaaccact gctccgtcgc ccggaagatg accgggttct tgcagcgcca gcagtgggga
     1801 taggagtgca atatcttttc ctcggccagg agcgcgccct tgcttctcaa cagctcgatg
     1861 atacccttgt tcgccttgaa gacctgctgt cccgcgaacc cgtccgcctc tttcgtgaag
     1921 cgccccttgc tgtcaacagg cgcgtaaacc tccaacccgt atttcagtcc ggcctcgtag
     1981 tcctcctggc cgtgtccggg cgcggtatgc actgcgccgg tgcccgcctc cagcgtcacg
     2041 tgatcgccca ggatgacgag cgattcgcgc tccggggtga tgaagggatg acgcgccttg
     2101 aggccttcga gcttcctgcc tttgaacttg tcgaggaccc ggtattccct gaagccgaac
     2161 ttctccatcg actgtttcaa aagaccttcg gccaggatga gcgcggaacc gccggtctcg
     2221 acggccacgt aatcgaactc aggatggagc gcgacggcca tgttcgcaac gagcgtccag
     2281 ggtgtcgtgg tccagatcgc gatcgccgcc gccttcccgt tcagggccgg gatggccttg
     2341 cctgcgtcgg gcagttcgaa ccgcacatac acggagggcg attcgtggtc agcgtactct
     2401 acctcggcct cggccagggc ggtctcgtca aagccgcacc agtacaccgg cttcttgccc
     2461 ttgtagacgc ttccggcggc cacgaacttt ccaagctcgc gcacgatggc ggcctcatag
     2521 gagaagtcca tcgtcagata gggatggtcc caatctccga aaacgccgag ccgcttgaac
     2581 tcctcgcgct ggacgttgat gaatttttcc gcgtactcgc ggcagagcct gcggatctcg
     2641 atctggctca tgccttcttt cttgggcccc aggctcttca agacctggtg ctcgatcgga
     2701 aggccgtggc agtcccagcc cggtacgtag ggactccgga acccttccat cgtcttgaat
     2761 ttgacgatga tgtccttcag gatcttgttc agggcatggc cgatgtggat gtgtccgttc
     2821 gcgtacgggg gcccgtcgtg caggatatag gtcccgcgtc ccgcagtctg ttcctgaagc
     2881 cggtcatgga tcttttcccg ccgccatttc tcgatgaccc gcggttcgag gtccttgagg
     2941 ttggccttca tcgggaaatc ggtcctgggt aaattcaatg ttgtcttata gtccatgctg
     3001 cgcgcacctt ccgttgccga gtccggcatg ccggctttca ttccgcattc cgcactccgc
     3061 attgggcatt tgagttagtc tgttatttcc tcgggatcgt attttttcgg ggtctctccg
     3121 gcatcgccct ggtcgatgtt atagagctgc ccggtgctcc tgcgcaccgt gtccgaatac
     3181 tggatgggct ccgtaccgcg gcggaacacg tcggtgacgg cattctcctg tccgtcacgc
     3241 gccaggagcc ccgtctcggg gtcgatcttg accgtcacca cgtcgtcgtc gggcgggatg
     3301 aagtcctcgg ccggcttgtc cgcaagggca ttggacataa ccgatatcca gatcgggagg
     3361 gcggcccgcg ccccggtttc tttgttgccg agcgacgccc tgggattgtc atagccgacc
     3421 cataccccgg tcacgatact gggcgtaaag cccacgaacc aggcgtccac gtacttgttc
     3481 gtggtgccgg tcttgcccgc gaccggtttc ccgagcaccc tggcagcccg ccccgtgcct
     3541 tcctccacca cgctcttcag gaggctgtcg acgagatagg ccgtcgtcgg gttcagggcc
     3601 tccacgggct ggggttcgta ggactcgagc acctggcccc tgctgtcggc gatgctcgtg
     3661 atcaggatcg gttccgagcg cacgccctgc gacgcgaagg tcgcgtaagc ggaggtaagc
     3721 tccagcggcg tcaccgccga ggaaccgagg gccgtcgtgt agtcatgact gatctggctc
     3781 ctgataccgg ccttgcgcgc gaaatcaatg gtcttgtcca ggccgatctt ctcgagaagc
     3841 gccacagtga ccgcattccg ggagagcgcc agcgccgtcc ttagtcgcat ggggcccagg
     3901 aaccgatcgt cgtagttctc cggcttccat cccttcttat cgtactcgct cttctcgtgg
     3961 attatcggca ggtccatcag gatggacgcg ggcgtaaacc cgttctcgag cgccgcgccg
     4021 tacacgaatg gtttgaacga cgatccgggc tggcgcctgg cgtccatcgc gtggttgaag
     4081 cctccctcca cgaagtcgta gccgccaacc agtgcgcgga tgtatcccgt atagggctcg
     4141 atggacacga cggccccctc cacgaggggg gtctggtcaa ggatgaagga agcgagctgc
     4201 tttttccggt cgaagtcctt gaaccggact ttgatgatat cgccgggctt gatgatttcc
     4261 gacggtttcc tgaactccgg ctcgccttcc ttccgtctct gcctggcgag agcccacgcc
     4321 atgtcgtcgc gtgcgatcac gccgaggagg cctcgtcccc tgaccgtgac ggatgtatct
     4381 ccgacctcga ggacctgcgc gttgaatcct tcgcccggct tgaccataac gcggaaggaa
     4441 ccggatttta cctggttccc cttaagctcc ttaaacccga cgcggccgcg gaaaccctga
     4501 cgcttgtcga gggcccggag tccttcatgg agcgccgaca ccgcggcgcg ctggaggtca
     4561 aagtcgattg acgtcgagac cttgaggccc ccctgataaa gcttgtcggt cccgtatttt
     4621 ttctcgacat acatgcgtac gtaatcgacg acgtgcggcg cgatgtcctc ctgagaccgc
     4681 agattctcga ggttcagggg ctggtcgtag gtctgcttcg cctgggcctc agtgatatat
     4741 ccctcctccg ccatccggtg gaggacgtgc atctgcctca tcttggtaag gtcgatgtcg
     4801 ccgtagggcg aataccccgt cggcgactgt ggcaggccgg ccaacagcgc ggcttcggca
     4861 aggttgacct cccagatgtt cttgccgaaa taggtctttg cggccatctg gacgccgtag
     4921 gccccgtgtc cgaagtagat cttgttcaga tagagttcga ggatatcctc cttcgtgagt
     4981 tctttctcga tccgtcgcgc aaggatgatt tccttgatct tcctcgtcaa ctttctctcc
     5041 ggcgagagga atataacctt ggtcaactgc tgcgtgatgg tgctgccgcc ctgcttgagc
     5101 cgcagggaga agatgtcctt cagggccgcg cgcagaatcc cgcggtaatc gatgccttta
     5161 tgctggtaat accgggggtc ctcggtggcc aaaagcgcgt tcaccatttg tttcggtatc
     5221 cgatgcagcg gcacgtaaat gcccttttca atcctgacct ggcccaggac gcgtccgtcc
     5281 ttcgcgagga tcatcgtgcc ggcgctcggc ttgtagtcct tgagcgattc tatcgtcggc
     5341 aggccgaaag acaggatgta gaacatcagc gcgagggtga ggaccccggc cacgaacaga
     5401 aggccgagga tcgcaagcgc ctttttgggc gtcaggccat tcacgaacgg aacggcaacg
     5461 ccaaaccaga aagccttgaa cttctcccag gctaagagtg taatcttttt gagcgtttca
     5521 agaagcttaa ccaacaggtt tttcctcctg caaaatcaga aacctcgtga aaatacgcgt
     5581 gattttaagc agcatcgatg ataataacat caagggatga caaaatcaaa cgaaaagttt
     5641 ttgccgcgtg agcataaaga aatggggaaa ggcatgtagc atctttcgga acgagatggc
     5701 ggcagtggta gagaaaggcc cctccgacag gactggtcac tccagcggtt tatgcagttt
     5761 gaccggactc gtctttttcc tgagcctcat attaagcatc tccacaatca ccgagaacgc
     5821 catggcaaaa taaatgtagc ctttcgggac atgaaggtcg aggccttcgg caatgagtgt
     5881 aacgccgata agtacgagaa agctcagggc cagcatcttg atcgtcggat gccggtccac
     5941 aaaggagccg atggcgccgg aagagaacat catcacgatc acggccagga ccacggcgat
     6001 gatcatgact gcaacttctt tggccagacc gacggcggtg atcaccgaat cgagggaaaa
     6061 gatgatatcc aggactgcta tctgcgcgag cgtgacagcg aaagccgcct cagcctttcc
     6121 ggtaatctcc tcctcagccc cttcaaggct gtcatggatt tccagtgtgc ttttccagag
     6181 gagaaacagg ccgcccacga tcaggatgag gtccctgccc gaaatttcct gcgcaaaaac
     6241 ggtaaacagc ggggtcgtca gtttcatgac ccagacgatg gaaaggagca ggccgatgcg
     6301 cgtcaacatc gccaggccga gccccagcac gcgggccttg ctccgctgcc gctccggcag
     6361 cttgcccaca agaatggaga tgaagatgat gttgtcgacg ccgagcacga tctcgagcgc
     6421 cgtcagcgtc acaagcgcga tccacgcctc aggggttgtc agccagtcca tacgttgctc
     6481 cttacttcct ctctttgtcc ctttcggtca ataacgacga tgcggtttta agagtatatc
     6541 ggggcagaat gggtttgtaa atcgaaattg gcaaataatt gctaagtcac ttctctattg
     6601 agccggcatg acgactcgag tggcccgatg atcatcgcaa ggtgacc
//
LOCUS       DASSLD010000661         2996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_146598,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000661 DASSLD010000000
VERSION     DASSLD010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146598"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFK23_11950"
     CDS             complement(<1..375)
                     /locus_tag="VFK23_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319843.1"
                     /translation="MQNRDNECSDGFIDAHVHIRDWTALEAVRAAGITAVRDAGLRHS
                     AEHGLSPHRTPGSGTLVISGGWALYKRGGYGAFFGLPAGSREEIRSEILKLKAAGADI
                     IKVMASGMVSLKSPGTITPGGFP"
     gene            complement(385..1092)
                     /gene="radC"
                     /locus_tag="VFK23_11955"
     CDS             complement(385..1092)
                     /gene="radC"
                     /locus_tag="VFK23_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET7319844.1"
                     /translation="MPDERKKSVKEWPEDERPRERLLKYGVEGLSDAQLLAIIIRNGR
                     DGRSAVDLGIELLEKFGGLDGIAQAGVSEFRGKNGVKGIGPAKIAEIKAAIELGRRHQ
                     KPSLAGATFCSSGDVISYYQPRMKDLKKEMFRCALLDTKNKIIQDEVVSVGTLTASLV
                     HPRETFRAAIRESAAAVIFIHNHPSGDIKPSQEDILLTRRLVQAGEVLGIQVLNHIII
                     GDGSHFSFRDNVLIQRP"
     gene            complement(1296..1424)
                     /locus_tag="VFK23_11960"
     CDS             complement(1296..1424)
                     /locus_tag="VFK23_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018154289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HET7319845.1"
                     /translation="MWRCTICGYIYDEAQEGTKFEDLPDDWSCPICNAPKDAFVRI"
     gene            complement(1558..2802)
                     /locus_tag="VFK23_11965"
     CDS             complement(1558..2802)
                     /locus_tag="VFK23_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HET7319846.1"
                     /translation="MRAIKIKPDIYWVGAIDWAVRDFHGYITPNGTTYNNYLILDRHV
                     TLVDTVKHDFSEYTIDNIKSLIDPAKIENIVINHIENDHASSLDRIMAFCPDATIYIT
                     ERGKKALDRFFDTARWKFQIVKTGHTLSTGSKTITFLETPMLHWPDSMMSYVKEDRLL
                     ISQDAFGQHLASSARFDDEYCDQYSISALEHAVRDYYANILMPFGTLVKAKLAEVRKL
                     GLEIDMIAPDHGIIWRKDPGKILQMYLDMANDKADLRIVIVYDTMWHSTEHMTIPLMQ
                     GIKSEGVDVDVLKLRSTPMSVAIKRVWESRAMLVGTPTLNNIMFPTVAEFLTHLRGLR
                     PKNRLAAAFGSFGWGGGAVKEAYESFKKMGLEVVEPGVEVLYRPSVEDDEKCFAFGKQ
                     FAIRTREYHKKFDGTLSAECRS"
BASE COUNT          551 a          870 c          877 g          698 t
ORIGIN      
        1 cgggaagccg cccggagtta tcgtcccggg gcttttaagg ctgaccatcc ccgaagccat
       61 gacctttatt atatcggcgc ccgcagcctt gagcttcagg atctccgatc gtatctcctc
      121 ccggctgccc gcgggaaggc cgaaaaacgc cccgtatcct ccccgtttgt agagcgccca
      181 tccgcctgag atgacgaggg tgccgcttcc gggtgtccga tgcggcgaaa gtccgtgttc
      241 ggcgctgtgc ctgagaccgg cgtcccgcac cgccgtgatc ccggcggccc ggaccgcttc
      301 aagcgcggtc cagtccctga tatggacgtg cgcatcgatg aagccgtcgc tgcattcatt
      361 gtcgcgattt tgcattgttg ccgatcacgg ccgctgtatc agcacgttgt cccggaagct
      421 gaagtggctt ccgtcgccga tgatgatgtg gtttaggacc tggatgccga gcacctctcc
      481 ggcctggaca agtcggcggg tcaggaggat gtcctcctga ctcggcttga tgtcgccgct
      541 cggatgattg tggatgaaga tgaccgccgc cgcggactcg cggatcgcgg ccctgaaggt
      601 ttcccgggga tgaacgaggg atgcggtgag ggtcccgacc gatacgacct catcctgaat
      661 gatcttgttt ttcgtatcga ggagggcgca ccggaacatc tctttcttga ggtccttcat
      721 tctcggttga tagtacgaga tgacgtctcc gctcgagcag aacgtcgcgc cggccaggct
      781 cggcttctgg tgccgcctcc ccaactcgat cgcggccttg atctccgcga tcttggcggg
      841 gccgatgcct ttgacgccgt tcttcccgcg aaactcgctg acgcccgcct gcgctatgcc
      901 gtccaggcct ccgaactttt cgagcagttc gatgcccaga tcgaccgcgg agcggccgtc
      961 tcggccgttc cggatgatga tcgcgagaag ctgtgcgtcg gaaagtccct cgacgccgta
     1021 tttcaggagc cgctctctcg gccgctcgtc ttccggccac tccttgacgc ttttttttcg
     1081 ttcatcgggc accggttagc ccctccgctg aaacgcgccg tcccggcagc gcatgaggtg
     1141 attatagaaa ctatgcgaaa ccctgtcaaa caaatttcaa ctgatgagaa cgcggggacg
     1201 gcgatccggc gggaaggatt gagacaacgg acggacaggt gcaacgaaaa aaggcggcct
     1261 gcggccgccc tccggttact tcaccgtgct ggttgtcata tccttacaaa cgcgtccttg
     1321 ggcgcattac aaatcggaca gctccagtcg tcgggaaggt cctcgaactt cgtcccctcc
     1381 tgggcttcgt catagatgta cccgcagatg gtgcatcgcc acatggttct gtcctccgtt
     1441 ttttaagtgc tgatactgga tacttgatgc ttgttgccgg ataacttcgt acaataaatc
     1501 atgacacgcg gtacagggga aggtttcatc cagcatctct gacaaagctc ttcaggatta
     1561 ggacctgcac tcggccgaca gcgtgccgtc gaattttttg tggtactcgc gggtgcgaat
     1621 tgcaaactgc tttccgaagg caaagcattt ctcgtcgtcc tcgacgctgg gccggtagag
     1681 tacctcgact ccgggctcga cgacctccaa tcccatcttc ttgaacgact cataggcctc
     1741 tttcaccgcg ccgccgcccc acccgaagct cccgaaggcc gcggccagcc ggttcttggg
     1801 ccggagcccc cggaggtggg tcaaaaactc ggcaacggtt gggaacatga tgttgttcaa
     1861 ggtcggcgtg cccacgagca tggcgcggga ctcccaaacc cgcttgatcg cgacactcat
     1921 gggagtcgat ctgagcttga gaacatccac atcgacccct tctgacttga tgccctgcat
     1981 cagggggatg gtcatgtgct cggtagagtg ccacatcgta tcatacacga tcacgatccg
     2041 gaggtccgcc ttgtcgttcg ccatatcgag gtacatctgg aggatcttgc ccgggtcctt
     2101 ccgccagatg atgccgtggt ccggcgcgat catgtcgatc tcgagtccga gtttccggac
     2161 ttccgcgagc ttggccttga cgagggtccc gaagggcatc aggatgttcg cgtaatagtc
     2221 cctgacggca tgctcgaggg ccgagatgga atactggtcg cagtactcgt cgtcgaaccg
     2281 ggccgaagac gcgaggtgct ggccaaaggc gtcctggctg atcaggagcc tgtcctcctt
     2341 cacgtagctc atcatggagt cgggccagtg gagcatcggc gtttcgagaa aggtaatcgt
     2401 cttcgacccc gtcgagaggg tatggccggt cttgacgatc tggaatttcc agcgggccgt
     2461 gtcgaaaaac cggtccaggg ccttcttccc gcgctcggtg atgtaaatcg tcgcgtcggg
     2521 gcagaacgcc atgatgcggt cgaggcttga ggcatgatcg ttttcaatgt gattgatgac
     2581 gatgttttcg atcttcgcgg ggtcgatcag gcttttgatg ttgtcgatgg tatactcgct
     2641 gaaatcgtgt ttcaccgtgt cgacgagtgt cacgtgccgg tcgaggatca gatagttgtt
     2701 gtaggtcgtg ccgttgggcg tgatataccc gtgaaaatca cggactgccc agtcgatcgc
     2761 ccctacccag taaatgtccg gttttatttt tatggcgcgc atggcggcct cccgttgact
     2821 ctggttaatc tggtgtgtgc agggtgcaag aaataaaaat cctctgcacg aatttctcaa
     2881 tcctaaacaa actcaacggc caaatgatgg aatttcacga ttcggtattg ttgagggttt
     2941 tgcactcggg atttagtgct tggtcttcac ggcgccttcc gcttctttcc gcaggg
//
LOCUS       DASSLD010000662         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_472430,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000662 DASSLD010000000
VERSION     DASSLD010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472430"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFK23_11970"
     CDS             complement(<1..273)
                     /locus_tag="VFK23_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET7319847.1"
                     /translation="MDLQQSIEIPKKIPLLPIRDIVVFPYMVLPLFVGREMSIKAIEV
                     ALEGNRMIFLASQKDINVENPSPSDLYSVGTVGVIMRMLKLPDGRIK"
     gene            complement(431..1300)
                     /gene="galU"
                     /locus_tag="VFK23_11975"
     CDS             complement(431..1300)
                     /gene="galU"
                     /locus_tag="VFK23_11975"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET7319848.1"
                     /translation="MSKPITKAIFPAAGLGTRFLPATKASPKEMMPLVDKPLIQYVVE
                     EAVSSGVEQVILITGRGKRAIEDHFDVAFELEEDLKAKGKHKLLSEVQRIANLVTFCY
                     IRQKKALGLGHAVLTAKRLVGGDPFAVLLGDDIIDAQVPALKQMMEVYQRYPATILAI
                     QKVPKNQTKNYGIIAGRKIEDGVYLVTDLIEKPSPDEAPSNLAIIGRYILVPEIFDAL
                     ENTQPGKGGEVQLTDGLRLLMKKLPIYAYEFQGVRHDAGDKLGFLKATVDFGLKNQEF
                     GEAFRDYLKKLKL"
     gene            complement(1410..>2048)
                     /locus_tag="VFK23_11980"
     CDS             complement(1410..>2048)
                     /locus_tag="VFK23_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HET7319849.1"
                     /translation="GEGTIGKLVNNDSMYNSIDSAAKGLTDYVSRIERMKTIVGFRSE
                     YMFPQSKTFASLELRPRPDTSYILELTSDPFGNFKRIEQTTTPPGGTVVTETFEDKFK
                     FSLEYAKRWGNLAVRLGLIESTGGIGADYYVWDDRFKFSVDSWNYNSNEPGNRNVHVK
                     TTATYGLGKTFFVNAGYDNFLNAQRKAPFVGMGLRFDDEDLKYLMGSMPIPK"
BASE COUNT          373 a          611 c          575 g          489 t
ORIGIN      
        1 cttgatgcgg ccgtcgggga gcttcagcat ccgcatgatg acaccgacgg tccccacgga
       61 atagaggtcg gatggggacg ggttctccac gttgatgtcc ttctgcgacg cgaggaaaat
      121 catccggttc ccttcgagcg ccacctcgat cgccttgatg gacatttccc tgccgacgaa
      181 cagcggcagc accatatagg ggaacacgac gatgtccctt atcggaagca aggggatctt
      241 tttcgggatc tcgatggact gctggagatc catgttcttc gtttcggtct tagccaaaca
      301 agcctccttg gattgtcgat tgttgattta tgattgttga ttgaaaaccg aaaatcgatg
      361 atcaagcggc gatcgacaat cgtcgatcca ttatttccaa tcgccaatca tcgatcgcca
      421 atcaacaatc ctaaagcttc agttttttca gatagtccct gaacgcctcg ccgaactcct
      481 ggttcttgag tccgaaatcc accgttgcct tcaggaatcc cagcttgtcg cccgcgtcgt
      541 gccggacgcc ctggaactcg taggcgtaga tgggcagttt cttcatcagg agcctgagcc
      601 cgtcggtgag ctggacctcc ccgcccttcc ccggctgggt gttctcgagt gcgtcgaata
      661 tctcgggcac caggatgtag cgcccgatga tcgccaggtt cgacggcgct tcgtcgggcg
      721 agggcttttc aatcaggtcc gtcacgagat agacgccgtc ctcgatcttc ctgccggcga
      781 tgatcccgta gttcttcgtc tggttcttgg ggaccttctg gatggcgagg atcgtcgccg
      841 ggtatcgctg gtacacctcc atcatctgtt tgagcgcggg gacctgcgca tcgatgatgt
      901 cgtcgccgag gagcacggcg aaggggtcgc cgccgaccag tcgttttgcc gtcagcaccg
      961 cgtggccgag gccgagcgcc ttcttctgcc gaatgtagca gaacgtcacc aggttcgcga
     1021 tgcgctgcac ctcagagagc agcttgtgct tgccctttgc cttgaggtct tcctccagct
     1081 cgaaggcgac gtcgaaatga tcctcaatcg cccgcttgcc ccgtccggtg atcaggatga
     1141 cctgctcaac gcccgacgat acggcctctt ccacgacata ctggatgagc ggtttgtcca
     1201 ccagcggcat catctccttg ggcgatgcct tggtggccgg caggaaacgg gtgcccaggc
     1261 ctgcggcggg aaaaatcgct tttgtaatcg gcttgctcat cctcattcct cgcgttcatt
     1321 ctatgaaact gacccggaaa tgtcaaggga agtaggtagc cgcacccttt agggtgcgtt
     1381 ctttccgcag gctgaagcct gcgcctacct tacttcggta tcggcatgct tcccatgaga
     1441 tacttcagat cttcgtcgtc aaaccgcagg cccatcccga cgaacggagc cttgcgctgc
     1501 gcgttcagga aattgtcgta gccggcgttc acaaagaagg tctttcccag accgtacgtg
     1561 gccgtcgtct ttacgtgcac gttccggttg cccggctcgt tgctgttgta gttccacgaa
     1621 tcgacgctga acttgaaccg gtcgtcccag acgtaataat ctgccccgat gccgcctgtc
     1681 gattcgatca gccctagccg gaccgcaagg ttcccccacc gcttcgcgta ctcgaggctg
     1741 aacttgaact tgtcctcgaa ggtttctgta acgaccgttc caccgggggg cgtcgtcgtc
     1801 tgttctatcc gcttgaagtt gccgaagggg tcggacgtca attcgaggat gtaagacgtg
     1861 tccggccgcg gcctgagctc cagcgaggcg aaggtctttg actgggggaa catgtactcg
     1921 ctccggaatc cgacgatcgt cttcatgcgc tcgatgcgcg agacatagtc cgtgagcccc
     1981 ttggcggcgc tgtcgatgct gttgtacatg ctgtcgttgt tgaccagctt gccgatcgtg
     2041 ccctctcc
//
LOCUS       DASSLD010000663         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1465665, whole genome shotgun sequence.
ACCESSION   DASSLD010000663 DASSLD010000000
VERSION     DASSLD010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465665"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFK23_11985"
     CDS             complement(<1..452)
                     /locus_tag="VFK23_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HET7319850.1"
                     /translation="MVLRKDFIVRVFFIALFLFPALGSSAAAERLPEARVIAVNDGDT
                     VTLRMDGKEYRSRLIGIDAPELGQEPWGKLSRGHLRKLLKDVKWRVTVETDIVKYDKY
                     DRILVYLWTPDGNMLNERMLRDGYALLFTIQPNSKYVDRLRKAQRAAR"
     gene            540..1367
                     /locus_tag="VFK23_11990"
     CDS             540..1367
                     /locus_tag="VFK23_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7319851.1"
                     /translation="MKKRLAIAAVIAALAVAAVVFYRYGNSGDQNGMVVSGNVEVTEV
                     NVGFKAAGRVAALFTDEGKAVKEGEKIASLDNAELESLVKQGRAAVRNAEAQFAKAKN
                     DYERFGKLFHDNVISPQQMDGARSAYEVATSQIELSRAALRTADVRLQDSVLFSPLSG
                     VVLRKNVEEGETVSAGTPVFTIGDLESPWIKVYVKEDKLGLVKLGQKAEVSVDTFPGK
                     KYEGTITFISSEAEFTPKNVQTREERVKLVFGIKVSVKNEKGELKPGMPADVKILLK"
     gene            1509..>2296
                     /locus_tag="VFK23_11995"
     CDS             1509..>2296
                     /locus_tag="VFK23_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7319852.1"
                     /translation="MNNPAIKTTKLTKSFNDVVAVNNLNLEIRKGELYGLVGPDGAGK
                     TTTMRLLAAIMDPTSGDAIVAGHSVLSEGERVREKIGYMSQRFGLYEDLTVMENILFY
                     ADLYDVPDKERPPRIERLLGFSNLSPFKERLAGNLSGGMKQKLGLACALVHTPEILFL
                     DEPTNGVDPVSRRDFWKILYDLLGGGVTIFVSTAYLDEAERCTRIGLMHKGGIMREDE
                     PKIIRKSLDLAMVEAWPDDARAALEPVRGIEGVHSVSLYGDRLH"
BASE COUNT          542 a          611 c          699 g          444 t
ORIGIN      
        1 tcgcgggcag cgcgctgcgc cttcctcaga cgatcgacat atttgctgtt cggctggatg
       61 gtgaagagca aagcgtaacc gtccctgagc atccgctcgt tcagcatgtt accatcgggt
      121 gtccagagat agaccaggat ccggtcatat ttgtcgtatt tgacaatgtc tgtctccacg
      181 gtcacgcgcc atttcacgtc cttcagcagt ttccgcaggt gcccgcggga gagttttccc
      241 cagggctcct gccccagctc cggcgcgtca atgccgataa ggcgcgagcg atattctttc
      301 ccgtccatcc ggagggtcac ggtatccccg tcattgacgg cgataacgcg ggcttcaggc
      361 aacctctccg ctgcggcgga ggaaccgaga gcggggaaca ggaagagggc gatgaaaaag
      421 acgcgtacga tgaaatcctt gcggagtacc atagcgggcc attatagcct cacgcttttt
      481 gacgtgtcaa actaattcaa ctataatgta ctagagcgtt gagaaaaggg ggatggttga
      541 tgaaaaaaag actggccata gcggctgtga ttgcggcgct cgctgtcgcg gccgttgtat
      601 tctaccgata cggcaattcc ggcgaccaga acgggatggt cgtatcgggc aacgtcgaag
      661 tgacggaggt gaacgtcggg ttcaaggcag ccggaagggt cgccgcgctt tttaccgatg
      721 aaggaaaggc cgtgaaggag ggcgaaaaga tcgcttcgct cgacaacgcg gagttggaaa
      781 gcctggtaaa acagggcagg gccgcggtcc ggaacgccga ggctcagttc gccaaggcaa
      841 agaacgacta cgaacggttc gggaagctct ttcacgacaa tgtcatctct ccccagcaga
      901 tggacggcgc acggtctgcc tacgaggtag cgacctccca gatcgaactc tcccgcgcag
      961 cgctcaggac cgcggacgtg agactgcagg actcggtgct cttttctccc ctgtcgggcg
     1021 tcgttcttag gaagaacgtg gaagaaggcg agaccgtgtc cgcgggaacg ccggtcttta
     1081 ccatcggcga cctggagagc ccctggatca aggtgtatgt gaaagaggac aaactggggc
     1141 tcgtgaaact ggggcagaag gcggaggtga gcgtcgatac gtttccgggg aaaaaatacg
     1201 agggaacgat cacgttcatc tcatcggagg cggaattcac ccccaagaac gttcagacca
     1261 gggaggagcg cgtgaaactt gtgttcggga tcaaggtaag cgtgaagaat gaaaagggcg
     1321 agctgaagcc gggcatgccg gcggatgtga agatacttct gaaataatct tcaaaaagcc
     1381 gtcttcccga atgcctttat cgggaatcca gagcctttga ttttatcgag gaaaagccac
     1441 agaattcccg ctggagactg tcccagcagc cgggatggaa atggcatcgt ttaaccatta
     1501 agaaatacat gaataatcca gccattaaga ctacaaaact cacaaaaagc ttcaatgatg
     1561 tcgtcgccgt aaacaatctc aatctcgaaa tccgcaaggg tgaactttac ggtctagtcg
     1621 gccccgacgg cgcgggaaag accaccacca tgaggctcct tgccgcgatc atggacccca
     1681 cctcgggaga tgcgatagtg gcgggacatt ccgtactctc cgaaggcgaa cgggtgcgtg
     1741 aaaagatcgg gtacatgtct cagcgcttcg gcctctacga ggacctgacg gtgatggaga
     1801 acatcctctt ttacgccgat ctctacgatg tcccggataa agagcgccct ccgaggatcg
     1861 agcggctgct cgggttcagc aacctctcgc cgttcaagga gcgtctcgcg gggaacctct
     1921 ccgggggcat gaagcagaaa ctcggccttg cctgtgcgct ggtgcatacg ccggagatac
     1981 tctttctcga cgagccgacg aatggcgtgg acccggtatc ccgcagggac ttctggaaga
     2041 tcctctatga tctcctgggg ggaggggtga cgatcttcgt ttccacggcc tatctcgacg
     2101 aagccgagcg gtgtaccagg atagggctca tgcacaaggg cgggatcatg agagaagacg
     2161 aaccgaaaat catcaggaag tctctcgatc tcgcgatggt cgaggcctgg ccggatgacg
     2221 cccgcgccgc gctcgagccc gtgcggggaa ttgagggagt ccacagcgta agcctctacg
     2281 gggaccggct gcacat
//
LOCUS       DASSLD010000664         4466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_211688,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000664 DASSLD010000000
VERSION     DASSLD010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211688"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1003
                     /locus_tag="VFK23_12000"
     CDS             38..1003
                     /locus_tag="VFK23_12000"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7319853.1"
                     /translation="MRDAEALAAESSAIAGAAPFQGKKFLVKYEDASTNTVVSGTTPE
                     FSEIRNFSPAKGRFFSEDEVKAAMRVAVVGKTVVNNLFEGRSAVGETIRINNVPFEVI
                     GVLEEKGVNAIGQDDDDQILIPITTVMRRLMNVIYVNNVYVAARDASLMDKAVADMTS
                     ILRERHHLKPGKEEDFSIQNQMDILRAEEETSETFTMLLGGIAAVSLLVGGVGILAIM
                     LISIKERVKEIGLRRAVGARRKDILLQFLIEALVLSVAGGLIGIVFGVSGSFVVGWTT
                     ELPTSVSALSIIVAFLFSAAVGLFFGIYPARKAAELDPIYALRSE"
     gene            1039..1419
                     /locus_tag="VFK23_12005"
     CDS             1039..1419
                     /locus_tag="VFK23_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HET7319854.1"
                     /translation="MKICFPVQKDDGLDSSVYGHFGSAPMFVVVDTETNGLATINNRD
                     QHHTHGSCNPMKALDNQKVDAIVVGGIGAGALTRLNQMGIRVHRAGAETIGENMTMFA
                     SRGLPELTVQGCCGGHGKGGGCAH"
     gene            1455..1802
                     /locus_tag="VFK23_12010"
     CDS             1455..1802
                     /locus_tag="VFK23_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319855.1"
                     /translation="MEILNQVRGYSYLLGVLKTQQADPFCPSCSAFSKTLTALRESLD
                     KFESEHYVEIQGLSGEFSRLFAEAKSGIAAVRHPDNPTGQKKAGNCKLPQGVCFTKLS
                     LAILQKVQPDSPA"
     gene            2079..3440
                     /gene="lat"
                     /locus_tag="VFK23_12015"
     CDS             2079..3440
                     /gene="lat"
                     /locus_tag="VFK23_12015"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HET7319856.1"
                     /translation="MIQPQDVKQTVSRHLLAEGFDVIFDEDRSAGSWLVDKRNGDHYL
                     DFFSMYASMAVGYNHPRLIEARGELGRLAVNKPTNSDVYTTALADFVETFSRVGMPAH
                     LPHAFFIDGGTLAVENALKTAFDWKVRKNLGRGLGREAGTKVIHFKQAFHGRSGYTLS
                     LTNTFDPRKTEFFPKFSWPRIVNPKVTFPLTAENLAAVRALEEEALREIRRAIEAEGE
                     DIAALIIEPIQGEGGDNHFRREFFHALRAICDEHEIMLIFDEVQTGVGLTGRFWAYEH
                     FDVLPDILCFGKKTQVCGILASRRVDEVGCNVFTERSRLGSTFGGNIVDMARFTRILR
                     IIEEDRLIDNARIQGSLLFTELQLLAHEFPGVISNVRGRGLMCAIDAPDTLTRNRLLK
                     AFLGEKLLMVACGERSIRFRPHLVVTADEIRQGAAIIRKVLAKGDYAGIEITPDACPR
                     GTT"
     gene            3440..>4466
                     /locus_tag="VFK23_12020"
     CDS             3440..>4466
                     /locus_tag="VFK23_12020"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7319857.1"
                     /translation="MNMAEKLRGLMRGRVIDDEKSLQQFSRDRSIYEIRPRAVVLPED
                     GEDARRLVEFARREGLAVTARGGGSGLAGSALGEGLVMALPKNGFWSAIGGFSGSGRS
                     ARVTASAGVYHNDLQKFLKERGFFLPADVTSAEISRIGGNIATRASGPHALKYGSIDR
                     FLESLEFITAEGELVDTANDATIPTRFREKLADLERRIIADDGAQAVLESRMSMKTAS
                     GYNLFAFLADLSPGRRIAKLLAGSVGTLGLITRAVLQAEVHEREAAAVLLYFDGLAEA
                     GRAVSSLRELDVAAIELISRETIRIMRDRTSLPGELAPDAHLLFVELAGPEVQKKVKS
                     VINLFHHD"
BASE COUNT          936 a         1320 c         1345 g          865 t
ORIGIN      
        1 ccggccgagg cagaccggca atacgacgtc cctcacggtg cgcgatgccg aggctctcgc
       61 cgctgaatct tccgccatag cgggagcggc cccatttcag ggcaaaaaat ttctcgtgaa
      121 gtatgaggac gcgagcacca acacggtcgt ttccggcaca acaccagaat tctccgaaat
      181 tcgtaatttc agtcccgcta aagggcgctt tttcagtgag gatgaagtga aggctgcaat
      241 gagggtcgcc gtcgtcggaa agacggttgt aaataatttg tttgaaggac gcagcgcagt
      301 cggagaaacg atccgtatta acaacgtacc cttcgaggtc attggagtcc ttgaggaaaa
      361 aggggtaaat gccattggtc aggacgatga tgatcaaatc cttatcccga ttaccactgt
      421 catgcgcaga ttgatgaacg tcatttacgt gaacaacgtc tatgtcgcag cccgcgacgc
      481 gtcgctcatg gacaaagcgg ttgccgatat gacctcgatc ctgcgcgagc gtcatcattt
      541 gaagcccggc aaggaagagg atttcagcat ccagaatcag atggacatcc tgcgagcgga
      601 ggaggaaacg tccgagacct tcacgatgct cctgggcggc atcgcggctg tgtcgctcct
      661 ggtcggcggc gtgggcattc tcgccatcat gcttatctcg atcaaggagc gggtcaagga
      721 aatcggcctc cgcagagcgg tcggcgcacg gcggaaggat atcctgctcc agttcctcat
      781 cgaagcactg gtcctcagcg tcgcaggcgg cttaatcggt attgtattcg gcgtttcggg
      841 ctcgttcgtc gtcggctgga caacagaact tccaacgagc gtatccgccc tctccatcat
      901 cgttgccttt ctgttctcgg ccgccgtcgg cctgttcttc ggcatctacc ccgccaggaa
      961 ggctgccgaa ctcgacccca tttatgcctt gcgatcggaa taaaaccatg agagaatact
     1021 acttacggag gaacaacgat gaagatatgc tttcctgtac agaaagatga cggactcgac
     1081 agctcagtat acggccattt cggttcggcg ccgatgttcg tagtcgtcga tacggaaaca
     1141 aacggactcg ccacgatcaa caaccgggac cagcaccaca cccacgggtc gtgcaacccg
     1201 atgaaggcgc tcgataatca gaaggtcgac gccattgtcg tcggcggcat cggcgcgggc
     1261 gcgctcacac ggctgaacca gatggggatc agggtgcatc gagcgggggc ggagacgatc
     1321 ggggaaaaca tgaccatgtt cgcttcgcgg ggccttccgg aattgacggt ccaggggtgc
     1381 tgcggcggac acggcaaagg cggaggctgc gcccactaag gcttttcaag aatcgaaaaa
     1441 ggaggaaatg ccccatggaa attctgaatc aggtgcgtgg ctattcctac ctgctcggcg
     1501 tactgaaaac ccagcaggcg gacccctttt gcccttcctg cagcgcattc agcaagaccc
     1561 tcacggcgct cagggaaagt ctggacaagt tcgaatccga gcattatgtc gaaatacagg
     1621 gcctttcagg tgagttctcc cgcctctttg cagaagcgaa gagcggaata gccgccgttc
     1681 ggcacccgga taacccgact ggccagaaga aagcgggaaa ctgcaagctg ccccagggag
     1741 tctgctttac aaaattgtcg ctcgcaattc ttcaaaaagt ccaacccgac tcaccagcgt
     1801 aagagagcgc cctgatgatc gtatggcaag ggtaagcgcc ggccgggaag tgtccagccg
     1861 gcgttttctt ttgggacgag caatgaagcg gcggccgtga ggtccgtctc ggcgcacctc
     1921 cggatccggg gccgcttccg cagaatcact cgtcgcttgc cgaacggata tccatcgctt
     1981 atactaggag tatcggccgt ccggttgatt ccgaaatgtt cagccggctc cgtaccatcc
     2041 tttttttcga aaccaaatcc ttctctccgg aggcgttcgt gattcagccg caagacgtga
     2101 aacagaccgt gtccagacat ctcctcgccg aagggttcga cgtcatcttc gacgaggaca
     2161 gatcggcagg ttcctggctc gtggacaaga gaaacggaga ccattatctc gatttttttt
     2221 ccatgtacgc gtccatggca gtgggctaca atcatccgcg tctgatcgag gcccggggcg
     2281 agctgggacg gctcgccgtg aacaagccga ccaattccga cgtgtatacg acggctcttg
     2341 ccgatttcgt cgagaccttt tcccgggtcg ggatgccggc ccatcttccc cacgccttct
     2401 tcattgacgg cggcaccctt gccgtggaaa acgccttgaa gacggccttc gactggaagg
     2461 tacgaaagaa cctgggccgc ggcctcggac gggaagccgg gaccaaggtc atccatttca
     2521 aacaggcctt ccacggccgc agcggctaca cgctttcgct caccaacacc ttcgatccgc
     2581 gcaagaccga gttcttcccg aaattttcct ggccgcgcat cgtcaacccg aaggtcacct
     2641 ttcccctgac cgcggaaaat ctcgcggcgg tccgcgcgct cgaggaagaa gccctgcggg
     2701 agatccgccg cgctatcgag gcggagggcg aagacatcgc ggccctgatc atcgaaccca
     2761 tccagggcga aggcggggac aaccacttcc ggcgcgagtt cttccatgcg ctgcgcgcga
     2821 tctgcgacga gcacgagatc atgctcatct tcgacgaggt ccagaccggc gtgggtttga
     2881 ccggcaggtt ctgggcctac gagcatttcg acgtcctgcc cgacatcctc tgtttcggca
     2941 agaagaccca ggtctgcggc atcctggcat cgcggcgcgt cgacgaggtc gggtgcaacg
     3001 tgttcactga aaggagccgc ctcgggtcga ccttcggcgg caacatcgtg gacatggcgc
     3061 gcttcacccg gatcctccgc atcatcgagg aagaccggct gatcgacaac gcccgcatcc
     3121 aggggtccct tttgttcacg gaactccagc tgctcgccca tgaatttccc ggggtgattt
     3181 cgaatgtcag gggccggggg ctcatgtgcg ccatcgacgc ccccgacacc ctgacgcgga
     3241 accggcttct gaaagccttt ctcggggaaa aattgctcat ggtggcctgc ggagagagga
     3301 gcatccgctt ccgtccgcat ctggtcgtca cggccgatga aatccggcag ggggccgcga
     3361 tcatccgcaa ggtcctcgcc aagggtgact atgccgggat cgagatcact cctgatgcct
     3421 gtccgagggg cacgacctga tgaacatggc ggaaaaactc cggggactga tgcgaggccg
     3481 cgtgattgac gatgaaaaat ccctgcagca gttttcacgc gaccggagca tctacgagat
     3541 caggccccgc gcggtcgtgt tacccgaaga cggcgaggac gcccgaaggc ttgtcgagtt
     3601 tgcgcggcgg gagggattgg cggtcacggc ccggggcgga ggatcgggcc tggccggaag
     3661 cgcgctcggc gaagggctcg tcatggccct gccgaaaaac gggttctggt ccgcaatcgg
     3721 cggcttttcc gggagcggcc gctcggctcg ggtcaccgcc tcggccgggg tctatcacaa
     3781 cgacctgcag aaattcttaa aggagcgggg gttctttctt ccggccgacg tcaccagtgc
     3841 cgagatcagc cgcatcggcg gcaacatcgc caccagggcg agcggccccc acgctttaaa
     3901 atacggctcc atcgaccgct ttctggagtc cctggagttc atcaccgcgg aaggggagct
     3961 ggtcgatacg gcgaatgacg caacgatccc gacgcggttc agggagaagc tcgcggacct
     4021 cgagcgaagg atcattgccg acgacggggc gcaggcagta ctcgagtccc gcatgagcat
     4081 gaagacggcg agcggctata atctttttgc ttttttggcc gatctgtctc cggggcggcg
     4141 tatagcgaag ctcctggcag gcagtgtagg aacgctcggt cttatcaccc gcgccgttct
     4201 tcaggccgag gtccatgagc gggaagcggc ggccgtgctg ctgtactttg acggcctggc
     4261 ggaggccggc agggcggtct cctctctgcg cgagctggac gtggccgcga tcgagctcat
     4321 aagccgcgag acgatccgca tcatgcggga ccgcacgtct cttccggggg agttggcccc
     4381 ggacgcgcac ctgcttttcg tggagctggc cggaccggaa gtacaaaaaa aagtaaaatc
     4441 cgtgatcaat ctctttcatc atgacg
//
LOCUS       DASSLD010000665         2954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1334342, whole genome shotgun sequence.
ACCESSION   DASSLD010000665 DASSLD010000000
VERSION     DASSLD010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334342"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="VFK23_12025"
     CDS             complement(<1..1453)
                     /locus_tag="VFK23_12025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET7319858.1"
                     /translation="MMLDALFNPRSIAVIGASHDPKKVGHAVLNNLIRHTFKGRLCPV
                     NPSGGEILGLKAYPTVSAIGDNIDLAVIAVPARIVPGTLRECAAAGIKSAVILSAGFK
                     EAGKEGTRLEEELRSISKERGIRILGPNCLGLINTANSMNATFAADMLPKGKIAFFSQ
                     SGAMGIAIMDWAIGNEIGFSKFISLGNKADLSEIDFIEYLIDDPDTDMILGYLEDVVD
                     GKRFMDIAHKATRVKPVVLLKSGGTEAGARAASSHTGALAGSDVAFDAAFRQTGIMRA
                     QGVQDLFDTALAFAEGKIPAGNGLLVITNAGGPGIIAADTAERLGVELPHMTRESIAA
                     MALKLPPNATVFNPVDVIGDATSERYAAVLEQASRDPNVDGMLVILTPQAMTDIDNTA
                     DVVIATSGTTDKPIIASFMGEARVRTAIKKLKDASIPNFSYPELAVKAFKRLSEHRAR
                     QNLEQEQPMCLEVNKGALRQKIDGFIAANKFQLG"
     gene            1801..>2954
                     /locus_tag="VFK23_12030"
     CDS             1801..>2954
                     /locus_tag="VFK23_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HET7319859.1"
                     /translation="MMGQEPHDILELIKSRHPEKFPAEEGVFSHIHPGNRIFIGTGCG
                     EPQYLVSALIKYVQSHPKAFFDTELLQVWTLGVAPYADAKFKDIFRHNSFFIGSNTRE
                     AVNRGIADYTPIFLSQVPALFQKKLVPVDVALIQTSLPDKHGYMSLGISVDIVKAATE
                     NAALVIAQTNALMPRVLGDTFINIRDVDFVIPHDEQLLEFEVTVSDEIAQKIGTYVSR
                     IVLDGDTIQVGYGSIPNAILTHLQSKKHLGVHTELLTDGIVMLMKRGVIDNSKKEVNR
                     GKTVAAFCMGKRETYEFLHDNPSIEFRPVNYTNNPTVLARQKNMTAINSALEIDLTGE
                     ATAESIGKLFYSGIGGQADFMRGAILSHGGKSILALQSTAENDEVSRIVPV"
BASE COUNT          652 a          827 c          841 g          634 t
ORIGIN      
        1 cgccgagctg gaatttgttc gcagcaatga atccatcgat cttctgccgt aacgcaccct
       61 tgttcacctc aaggcacatc ggttgttctt gttcgaggtt ttgccgtgcc ctatgctccg
      121 acagccgttt gaaggccttc accgcgagct cgggatagga gaaattcggt atcgaagcgt
      181 ccttaagctt cttgatagcc gtccgcacac gtgcttcacc catgaaagag gcaatgatcg
      241 gtttgtccgt tgtcccggat gtagctatga cgacatcggc cgtgttgtcg atgtccgtca
      301 tggcttgagg agtaaggatc accagcatgc cgtcgacgtt ggggtccctg gaagcttgct
      361 cgagcacggc tgcatatcgc tcggaagtgg cgtcaccgat cacatccacg ggattaaaaa
      421 cggtggcgtt gggaggcagc ttcagggcca tggccgcaat gctctctctg gtcatgtgcg
      481 gcagttcgac gccgagtcgt tctgccgtgt ccgctgcgat gattcctggg ccgccggcgt
      541 tcgtgatcac aagcagtccg ttccctgcag ggatcttccc ctcagcaaag gccagggccg
      601 tgtcgaagag atcctggacg ccctgagcac gcatgatccc cgtttgcctg aaagcggcgt
      661 caaaggcaac gtccgaaccg gcgagtgcac cagtatggga cgatgccgcg cgggccccgg
      721 cctcggtgcc accggatttc aggaggacga cgggctttac cctcgtcgcc ttgtgcgcga
      781 tgtccatgaa ccgtttccca tcaaccacgt cctcgaggta tccgaggatc atgtccgtgt
      841 ccgggtcgtc tattaaatat tcgatgaagt cgatctccga gagatcggcc ttgttcccga
      901 gactaatgaa cttggagaat ccgatctcat ttccaatcgc ccagtccatg atggcgatgc
      961 ccatggcgcc ggactgggaa aagaaggcaa tcttgccttt cggaagcatg tccgcggcga
     1021 aggtggcatt catgctgttc gccgtgttga tgaggccgag acagttcggg ccgagaatgc
     1081 ggatgccccg ctctttgctg atgctcctga gctcttcctc cagcctggtg ccttccttgc
     1141 cagcctcctt gaatcccgca cttaggatga ccgctgactt gatcccggcg gcagcgcatt
     1201 ccctaagcgt gccgggtacg atgcgcgcgg gcacagcgat gacggcaaga tcgatgttat
     1261 caccgattgc cgagaccgtg ggataggctt tcaggccaag gatttcgcca cccgatggat
     1321 tcacggggca gagcctgcct ttgaatgtat gtcggatcag gttattgagg accgcatgac
     1381 cgactttctt cggatcatga gatgcaccga tgacggctat ggagcggggg ttgaagaggg
     1441 catcgagcat catgtattaa gtttagttga gaaaaagctg atggtcaagg ctggaaagcg
     1501 gatttcatca ggtcgctttc tgaggtgatg agacgccggc tgtcccagga gcattcggtg
     1561 ccattcatcg tcattatgaa cgatatgcgg gagagtgcac tcttgttgtt tatcgtgaga
     1621 ttgccacaac taaaatcaag gttctcgtga ggatttattc gtggctttgt gggcctgtct
     1681 ggaagctgaa caaacaggga ttctagcaga aaaggagcat aaacaaagtg tgaataacgc
     1741 ataaacttgt caagtgagga gtgccctttc tgctatactt aaagatggca ggagagtgcc
     1801 atgatgggac aggaaccgca tgatatactg gagctgataa aatcgcgcca ccccgagaag
     1861 ttcccggcgg aggaaggcgt cttcagccac atccatccgg gcaacaggat cttcatcgga
     1921 acgggatgcg gcgaacctca gtaccttgta agcgcactca tcaaatacgt tcagtcccac
     1981 cccaaggcct ttttcgacac agagctgctg caggtctgga cactgggtgt agcgccatat
     2041 gccgatgcaa agtttaagga catcttccgg cataactcgt tctttatagg gagcaacacg
     2101 cgcgaggccg tgaaccgtgg catcgcggac tataccccca tattcctgtc ccaagtgccc
     2161 gccctgttcc agaaaaagct cgtgcccgtg gacgttgccc ttatccagac ctccctaccg
     2221 gacaaacacg gatacatgag ccttggcatc agcgtggaca tagtgaaggc ggccacggag
     2281 aatgccgcgc tcgtcatcgc acagacgaac gcgctcatgc cgcgggttct cggagatacc
     2341 ttcatcaata tcagggacgt ggacttcgtc atcccgcacg acgagcagct cttggagttc
     2401 gaggtcaccg tgtcggacga gatcgctcag aagatcggga cctacgtctc ccgtattgtg
     2461 ctggatggcg atactatcca ggtcggatac gggagcatac cgaacgccat cctcacacac
     2521 ctccagagca aaaagcacct cggcgtgcac acagaacttc tgaccgatgg catcgtgatg
     2581 ctcatgaaac ggggagttat cgacaattct aaaaaagagg tgaaccgcgg caagacagtg
     2641 gcggccttct gcatgggcaa gcgggagacc tatgagtttc tccacgacaa cccgtccatc
     2701 gagttccggc cggtgaacta taccaacaac ccaacggtgc tcgcccgcca gaagaacatg
     2761 acggcgatca acagcgccct ggaaatcgac ctcacgggcg aggccacggc cgagtccatc
     2821 ggcaagctgt tctacagcgg catcggcggc caggcggact tcatgcgcgg cgcgatcctg
     2881 tcccacggcg gcaagtccat cctggcgctc caatccaccg ctgagaacga cgaagtgtcg
     2941 cgcatcgttc cggt
//
LOCUS       DASSLD010000666         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_90256,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000666 DASSLD010000000
VERSION     DASSLD010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90256"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..222)
                     /locus_tag="VFK23_12035"
                     /pseudo
     CDS             complement(94..222)
                     /locus_tag="VFK23_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            343..636
                     /locus_tag="VFK23_12040"
     CDS             343..636
                     /locus_tag="VFK23_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HET7319860.1"
                     /translation="MKQKNWKELPHAGTVLKAGNANEYKTGSWRSFKPRWIEENCIQC
                     LFCWIYCPDAAVRVKDEKMIGFDYDHCKGCGVCAMECPGKKGNKAIVMEEEGK"
     gene            638..1831
                     /gene="porA"
                     /locus_tag="VFK23_12045"
     CDS             638..1831
                     /gene="porA"
                     /locus_tag="VFK23_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HET7319861.1"
                     /translation="MATMTKTKTVAVTGNQAVAEAFRQLNPDVMPAYPITPSTGIMES
                     FTKFVSDGKVDTETILVESEHSAMSACIGASAAGGRVATATSAQGLALMWEMLHIASG
                     YRLPILMALVNRALSAPLNIHGDHSDGMGMRDCGWIQIFCENAQEAYDNTIMAYRIAE
                     HKDVRLPIAVNMDGFIISHSIESMQFMTDEDVKKFVGEYIAIDPLLDIDNPKSIGALV
                     LPDYYMEHKRAQHQAMLNVMKVVHDVSAEFAKLTGRKYDLFESYRLEDAEVAIVALNS
                     AAGTIKDEIDHYREKGVKAGLLKPRMFRPFPYEEVGAALKNAKAVCVMDRADSYGGFG
                     PLFMEIASAVYPHRQGPVLFNKIYGLGGRDLMPADVDQVIDEAVEVAKTGKVKLAKEY
                     ITVRN"
     gene            1919..>2323
                     /locus_tag="VFK23_12050"
     CDS             1919..>2323
                     /locus_tag="VFK23_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7319862.1"
                     /translation="MTTTQEKPMTLKSLSHKEDLLTGGHRMCSGCGAPTIVHQVLLAI
                     DEPVVIANATGCLEVSTCLFPYTAWRVPWMHSAFENAAATASGIETMYRALRKKGKIK
                     KDIKFIAFGGDGGTYDIGFQALSGAMERGHQLL"
BASE COUNT          526 a          669 c          686 g          442 t
ORIGIN      
        1 gctggcatga catcggggtt cagctggcga aaggcctcgg caacagcctg gttgccggtc
       61 accgcaacga tcttggtcgt cgtgattgcc atgttatttg ccctcctctt ccatgacaat
      121 ggccttgttg ccttttttgc ccgggcattc catggcgcag acaccgcagc ccttgcagtg
      181 gtcgtaatcg aaattgaccc ttttttcatc cttcacggga atcgttggga tggacgtact
      241 cctggaggat gtcaagggca gcttcgggaa gaaattcgcg cagaagatca tcgatgggaa
      301 tctcgccgcg gtgaagcgcg gatacgagga ggtaaggcag ggatgaaaca gaagaactgg
      361 aaagagctgc cgcacgctgg cacggtgctg aaagccggca acgcgaatga atataaaacg
      421 ggatcatggc gctcgttcaa gccgcggtgg atcgaggaga actgcatcca gtgcctgttc
      481 tgctggatct actgcccgga tgccgcggtc cgggtgaaag acgagaaaat gatcggcttc
      541 gattatgacc actgcaaggg ctgcggcgta tgcgccatgg aatgcccggg taagaagggc
      601 aacaaggcca ttgtcatgga agaggagggc aaataacatg gcgacgatga ccaagacgaa
      661 gaccgtggct gtcaccggca accaggccgt tgccgaggcg ttccgccagc tgaaccccga
      721 cgtgatgccg gcctatccca tcacgccgtc tacgggaatc atggaatcat tcaccaagtt
      781 cgtttccgac ggcaaagtgg acaccgagac gatcctcgtg gagagcgaac acagcgccat
      841 gagcgcctgt atcggagcct ccgccgcagg cggccgcgtg gcgacggcaa caagcgctca
      901 gggcctggcc ctcatgtggg agatgctgca cattgcctcg ggctaccggc tccccatcct
      961 gatggcactc gtgaacaggg ccctctctgc cccgctcaat atccacggcg accactcgga
     1021 cggcatgggc atgcgcgact gcggatggat ccagattttc tgcgagaacg cccaggaagc
     1081 ttatgacaac accatcatgg cctaccggat tgccgaacac aaggatgtgc gtctgccgat
     1141 cgcggtcaat atggacggtt tcatcatcag ccactcaatc gagagcatgc agtttatgac
     1201 cgacgaggac gtgaaaaaat ttgtcggcga atatattgcg atcgatccgc tcctcgatat
     1261 cgacaacccc aagagcatcg gcgccctggt gcttcctgac tattacatgg agcacaagcg
     1321 cgcccagcac caggccatgc tgaatgtgat gaaggtcgtg cacgatgtgt cggctgaatt
     1381 tgcaaagctc accggccgta aatacgattt gttcgagtct taccgtcttg aggatgccga
     1441 ggtcgcaatc gttgccttga actccgcggc aggtaccatc aaggacgaga tcgaccacta
     1501 ccgcgaaaaa ggcgtaaaag ccggcctcct gaagccccgc atgttccgtc ccttccccta
     1561 tgaggaagtg ggcgcagcgc tcaagaacgc aaaggccgtc tgtgtcatgg accgggcgga
     1621 ttcctacggc ggcttcggcc ccctcttcat ggagatcgcc tcggccgtgt acccccaccg
     1681 gcaggggccg gtgctcttca acaagatcta cggactgggc ggccgggacc tgatgccggc
     1741 ggatgtggac caggtgatcg acgaggccgt cgaggtcgcc aagaccggaa aagtgaagct
     1801 tgcgaaggaa tatatcacgg taaggaacta gattcttttg acgggattac aggatggaca
     1861 ggatgaatca catcggcttt tatcccgtta tcctgtctaa gaattcaagg agaatagaat
     1921 gaccacgacc caggaaaaac caatgacctt gaaatcgctg tcgcacaaag aggacctctt
     1981 gaccggcggg caccggatgt gttccggctg cggcgcgccc accatcgtcc accaggtgct
     2041 cctcgccatc gacgaaccgg tcgtcattgc gaacgccacg ggctgcctcg aggtctcgac
     2101 ctgtctcttc ccctacaccg cctggcgcgt gccctggatg catagcgcct tcgagaacgc
     2161 ggcggccacg gcctccggca ttgagaccat gtaccgggcg ctccggaaaa aagggaagat
     2221 caagaaggac ataaagttca ttgcgttcgg cggcgacggc gggacctacg acatcgggtt
     2281 tcaggccctt tccggcgcca tggagcgcgg ccaccagctc ctc
//
LOCUS       DASSLD010000667         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1418457, whole genome shotgun sequence.
ACCESSION   DASSLD010000667 DASSLD010000000
VERSION     DASSLD010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418457"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..843)
                     /locus_tag="VFK23_12055"
     CDS             complement(61..843)
                     /locus_tag="VFK23_12055"
                     /inference="COORDINATES: protein motif:HMM:NF015566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET7319863.1"
                     /translation="MPMIDSHMVLAILGVAVIGGIIGLDRTAAGQFMISQPIVAAPFT
                     GWVLGDMTAGMVIGATLELIWVLDIPVGTFVPANATVCAVSATAIAALGNPGGGAPLP
                     DIGFSVLLTAAMVPVTRKADGLIRAWNSRLSDRVLAATARDRGRELVRAQFAGLTFFF
                     LKSFVLCGIFVLVGIGALWIFGQFPGAVRRALSLFVKLLPLLGVAIVVRKLSIKALDQ
                     YFFAGFAAAALFGQAVHAPALVVLLLTSAAGWLGARYRERHA"
     gene            complement(1063..1554)
                     /locus_tag="VFK23_12060"
     CDS             complement(1063..1554)
                     /locus_tag="VFK23_12060"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET7319864.1"
                     /translation="MIALIRIDDRLIHGQIVEGWVNYLKATSILVADDAVAGNTLQRT
                     IMELSVPQGLKVFIGSVEEVCIRARTAGQDAGHAIVVFSNPEDVLRALNAGMECRSLN
                     IGGMHYMPGKRKLMDVLAVNDADLDALRAISGKGIKIAIQTVPTQRPVPLEKVLDAAS
                     QKK"
     gene            complement(1562..1963)
                     /locus_tag="VFK23_12065"
     CDS             complement(1562..1963)
                     /locus_tag="VFK23_12065"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter"
                     /protein_id="HET7319865.1"
                     /translation="MIGLVVVCHEDMGAELVKAAEMIVGKIDAVATVSVKQESAPEIL
                     RDEIQAAIKKVDRKKGIMLFTDMFGGTPSNIALAFLGETTEVVTGVNLSMLIKFANHR
                     DEKHLADLARLVQEAAQKSIVIASQMLRGKK"
     gene            complement(2060..>2860)
                     /gene="rapZ"
                     /locus_tag="VFK23_12070"
     CDS             complement(2060..>2860)
                     /gene="rapZ"
                     /locus_tag="VFK23_12070"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET7319866.1"
                     /translation="KNFEDIGYYCVDNLPLELLPAFIEFVQRTEEASHSAIGLDIREK
                     GFPDRFPPLYGEIKARGSAIELVFLEASDHALVRRFSETRRPHPLARGATPLLDGIRL
                     EREALGEVKKLADWIIDTTDYTVHDLRQVVERRYAEADTGRPMAVTFVTFGYKYGVPY
                     ELDLLFALRFLPNPHFVPDLRPLTGENERIREYIMAGPDAGEFLERLLGFLEYLLPRY
                     QREGKSYLTIGFGCTGGKHRSVALALLAADRLRKKGYDVNVKHRDIGI"
BASE COUNT          585 a          895 c          825 g          555 t
ORIGIN      
        1 gacgcctgaa tgagcaacgc ccgttgaaac acggcgaaga ggtcgaaaat tcctatgatc
       61 ttacgcatgc cgttcacggt acctcgcacc cagccacccg gcggccgacg tgagcagcaa
      121 cacgacgagc gcaggagcgt ggacggcctg cccgaagagc gctgcggccg caaaaccggc
      181 gaaaaaatac tgatcgagcg cttttatcga cagcttgcgc acgacgattg ccacccccag
      241 caggggaagc agcttgacaa acagggacag cgcccggcga accgcgccgg ggaattgccc
      301 aaagatccag agcgccccaa tcccgaccag gacaaagatg ccgcagagca cgaatgattt
      361 cagaaagaaa aatgtcagac cggcaaactg ggcccgtacc agttcccggc cccggtccct
      421 ggccgttgcc gccaggacac gatcggacag acgagagttc cacgccctga tcaggccgtc
      481 ggcctttctg gtgaccggta ccatcgcggc tgtcaggaga acgctgaaac cgatgtccgg
      541 cagcggcgcg ccgcctcccg ggttcccgag ggccgcgatc gccgtggccg acaccgcgca
      601 gaccgtggcg ttggcgggga caaatgtccc cacggggatg tcgaggaccc agatcaattc
      661 aagcgtcgct ccgatgacca tgcctgcagt catgtccccg aggacccacc ccgtgaaagg
      721 ggccgcgacg atgggctggg atatcatgaa ttggcctgcg gcggtcctgt cgaggccgat
      781 gattccgccg atcacggcga caccaaggat ggcaaggacc atatgggagt cgatcatcgg
      841 cataaaaacc ctgcggcaat acgttcgtgt cattcccgcc tgcacccagc gcttacgagc
      901 ctgtggccag tgcctatgag tgcgccgcgc gaatacaggc catgcggcag agcgaatgca
      961 gggtgaatgc ttctatcggg aatccagttt atcaggatca cgaaaaatcg gagaccccgg
     1021 atcccggctt gaaccatgcc ggaatgacga ctaaatagaa aatcattttt tttgcgaagc
     1081 agcgtcaagg accttttcca gaggaaccgg ccgctgcgtc ggcaccgtct ggatggcgat
     1141 ttttattcct ttgcccgaga tcgcccgcag ggcgtccagg tccgcgtcgt tcaccgccag
     1201 tacatccatg agcttgcgtt ttcccggcat gtagtgcatc ccgccgatat tcagcgaacg
     1261 gcactccatc cccgcattca gggcccgcag aacgtcttcg gggttcgaga acaccacgat
     1321 ggcgtgtccg gcgtcctgcc ccgcggtgcg cgcccggatg cagacctcct ccacggatcc
     1381 gataaatacc ttcaacccct gcgggaccga caattccata atggtgcgct gaagggtgtt
     1441 tcccgcgacg gcgtcgtcgg cgaccaggat actcgtcgcc ttcaggtaat tcacccagcc
     1501 ctccacgatc tgaccgtgga tcaggcggtc atctattcgg atgagggcga tcatgccgga
     1561 attatttttt ccctctcagc atctggctcg cgatcacgat gctcttctgc gccgcttcct
     1621 gcacgagcct tgcgagatcg gccagatgtt tctcgtcccg gtggttcgcg aacttgatga
     1681 gcatcgacag gttgacgccc gttaccactt ccgttgtctc tccgagaaaa gcgagggcga
     1741 tgttggacgg cgtcccgcca aacatgtccg tgaacagcat gatccccttt ttccggtcaa
     1801 ctttttttat ggcggcctgg atctcgtccc ggagtatctc cggcgcgctc tcctgtttta
     1861 cgctgactgt cgccacggca tcgatcttgc cgacgatcat ctctgcggcc ttcaccagtt
     1921 cggcgcccat gtcctcatga cacaccacta cgagaccgat catgccatta tctccttttt
     1981 ctgttcaagg tgcggctctg cggcctggcg gcctcgccgc atcgaatgcg gaatccgcaa
     2041 ccgcacgtcc agaatcactt cagatcccga tgtcccgatg tttgacgttt acgtcatagc
     2101 cctttttccg gagccggtcc gcggccagca gggcgagggc caccgaacga tgcttcccgc
     2161 cggtgcaccc gaagccgatg gtcaggtagc tcttcccctc gcgctgatac cgcggcagga
     2221 gatactcgag aaaccccagg aggcgttcga gaaattctcc ggcatcgggc ccggccatga
     2281 tgtattccct gatccgttcg ttctcgcccg tcagcggtcg caggtccggg acgaaatggg
     2341 ggttcggcag gaaacgcagg gcgaaaagaa gatcaagctc atagggaacg ccgtacttgt
     2401 agccgaaggt aacgaaggtc accgccatgg gcctgcccgt gtccgcctcg gcatagcgac
     2461 gctccacaac ctggcgcaga tcatggacgg tgtaatccgt tgtgtcgatg atccaatcgg
     2521 cgagtttttt cacctcgccg agggcctccc gctccaggcg gatgccgtcc agcaggggcg
     2581 ttgcgcctct cgccagagga tgaggtctgc gtgtttcgct gaaccgacgt acgagcgcat
     2641 ggtcggaggc ttccagaaaa acgagctcaa tggcgctgcc gcgggccttg atctcgccgt
     2701 agagtggagg aaagcggtca gggaacccct tctccctgat atcgagtccg atcgccgaat
     2761 gcgaggcttc ttctgtccgc tgtacaaact caatgaaggc ggggagaagc tccaagggga
     2821 ggttgtcgac gcagtaatac ccgatgtcct cgaaattttt
//
LOCUS       DASSLD010000668         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1253954, whole genome shotgun sequence.
ACCESSION   DASSLD010000668 DASSLD010000000
VERSION     DASSLD010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253954"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VFK23_12075"
     CDS             <1..896
                     /locus_tag="VFK23_12075"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7319867.1"
                     /translation="QIFHSSRPVIEGLTSPSSCWWASTCVIIKTTGEREGPRENFMAR
                     IFITGSADGIGLMEARMLVERGHEVVVHGRNKKRANEALSKVPGALTAIAGDLANIAE
                     TKSLADQVNELGPFDAVIHNAGIYLDPQGIRTVDGLPLVFAVNSLAPYILTCMIEKPK
                     RLVYTSSGLHRNGDATLTDLTWSSRSWQDSSAYADSKLQNVILAFAVARKWPDVLSNA
                     VDPGWVATRMGGQGAPDSIEEGSRTQVWLTVSREKEALVSGKYFYHKHLRSFHPAAAD
                     PAVQEKYLSECARLSGVTIPG"
     gene            complement(1174..1563)
                     /locus_tag="VFK23_12080"
     CDS             complement(1174..1563)
                     /locus_tag="VFK23_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319868.1"
                     /translation="MILRYFLAWFGMMVLAIVNGGLRDFAYKAYVGDLPAHQISTVVL
                     LALLAGYIWFLTRIWPIKSARQAWIIGVMWFLMTEVFEFGMGLAQGDSWSELVYAYNV
                     FAGQVWILIPLWVLVGPYLFFRFVQKK"
     gene            1772..1969
                     /locus_tag="VFK23_12085"
     CDS             1772..1969
                     /locus_tag="VFK23_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319869.1"
                     /translation="MQRTEEFFAELRGNALVRFTEGEGHEHHLLFVAVRIVLMINGGG
                     MWSSDQAIATRIGSGKQQTGK"
BASE COUNT          490 a          526 c          598 g          418 t
ORIGIN      
        1 atcagatatt ccattcctca aggccggtaa tcgaaggact aacgagcccg agctcctgct
       61 ggtgggcctc cacctgcgtt ataatcaaga cgacgggaga acgtgaaggg cccagggaaa
      121 acttcatggc aagaatattc attacaggct ccgctgacgg gattggtctc atggaggcgc
      181 ggatgctggt cgagcggggc cacgaggtcg tagtgcatgg ccgcaacaag aagcgggcga
      241 acgaggcgct ttcaaaggtc ccgggcgcgt tgaccgcgat tgcgggcgat cttgcgaaca
      301 tagccgagac gaagtccctc gcggaccagg tgaacgagct gggaccgttc gatgcagtga
      361 tccataacgc cggcatctat ctcgaccccc agggcatccg gactgtcgac ggcctgccgc
      421 tggtctttgc cgtgaactcg ctggccccct atattctgac ctgcatgatc gagaagccga
      481 agcgcctggt ctatacgagc tcgggtctcc atcgaaacgg cgacgcgaca ctaaccgacc
      541 ttacatggag cagccggtca tggcaggatt cgagcgccta cgccgattcg aagctgcaga
      601 acgtcattct tgcctttgcc gttgcacgca aatggcccga tgtattgtcc aatgcagttg
      661 atcccggctg ggtggctacc aggatgggag ggcagggagc gcccgacagc atcgaggagg
      721 ggtcgaggac gcaggtgtgg ctcacggtga gccgggagaa ggaagccctg gtctcgggaa
      781 aatatttcta tcataagcat cttcggagct ttcacccggc tgcagccgat ccggctgtgc
      841 aggaaaagta tctttcagaa tgcgcccgat tgtccggagt aacgatcccc ggttagtacc
      901 aagaggggat cgtagtttgg agtaggaagt tcggagttcg gagaaggaag ttcgaagttc
      961 ccccgataaa ggcgttcggg gggaggctcc gttcggagcg aaagatacca gtttgaacca
     1021 gaaaagctgt ctcgcgcaga ggcgcaagga cgcagaggaa attttaaatc tgctaattag
     1081 tggtctgcct ttctttgcgc cttggcgttg cctaggggca cctacttttg gctcggcgcg
     1141 aaaatatttt tgccatttta gtacctgccg gcatcacttt ttttgaacaa agcggaagaa
     1201 caagtacggc ccaaccaaca cccataacgg aatgagaatc cagacttgtc ccgcaaatac
     1261 attgtatgcg taaacgagtt cgctccatga gtcgccctga gcgagtccca ttccaaactc
     1321 gaaaacctcg gtcatcagaa accacatgac gccgatgatc cacgcttgcc ttgccgactt
     1381 gatgggccag atccttgtga ggaaccagat gtagcctgcc aataaggcga ggagaacgac
     1441 tgtggagatc tggtgggccg ggaggtcgcc gacgtaggcc ttgtacgcga aatcgcgcaa
     1501 gccaccgttg acgatcgcaa ggaccatcat gccgaaccat gcgagaaaat acctgaggat
     1561 catgagatac ttccttttgt cgatggtagc tctcaggacg gctcttccgg gccgtgacgc
     1621 gctgaacgca ggagaacaac agctaacacc atcatccaca gcatgtaacc atagatgttg
     1681 atgcgctcac tcacgcctcg agctgtctga ctatcccgct ctttccagtg tatcaaattg
     1741 ctctgcaatc tttttgcggc cctggacagg tgtgcaaagg acggaggaat tcttcgcgga
     1801 gctgagggga aatgcactgg tccggtttac ggaaggggaa gggcatgagc atcacttgct
     1861 gttcgtggcg gtcaggatcg tgctgatgat caacggcgga ggaatgtggt cgtcggacca
     1921 ggccattgct acgaggatcg gctccggaaa acaacagacc ggaaagtgat gaacgcgcta
     1981 tacttcatac agatgaatac agtgaaaagt aaacgaggaa cttgtgcaat cc
//
LOCUS       DASSLD010000669         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_863916,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000669 DASSLD010000000
VERSION     DASSLD010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863916"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VFK23_12090"
     CDS             <1..824
                     /locus_tag="VFK23_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET7319870.1"
                     /translation="FVVSIIRSFFPPERTKRILSHKREFIGNILAALLGIVTPFCSCS
                     AVPLFIGFVEAGVPLGVTFSFLISSPMVNEVAVVLLWGLFGWKVAVIYMSAGLSVAIV
                     AGFIIGKLKLEKWVEEYVYKIQSLGAAIGTVRQTFKDRLVYARANTIEILQKVWLYVV
                     IAIGIGGFIHGFAPDDFLAKYAGNGNPFAVPLAVAIGVPLYSNAAGVIPIVYALMEKG
                     MSIGTVLAFMMAVTALSLPEMIILRKVIKIPLLVVFVSIMTLTIIAVGYLFNAII"
     gene            835..1077
                     /locus_tag="VFK23_12095"
     CDS             835..1077
                     /locus_tag="VFK23_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018057622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET7319871.1"
                     /translation="MEIKVLGPGCTNCHKMEEMAKQAVKELGISAEVTKITDIGDIVR
                     HGILSTPGLVVNGKIKHSGKPLPSLDKVKELIKSEA"
     gene            1113..1490
                     /locus_tag="VFK23_12100"
     CDS             1113..1490
                     /locus_tag="VFK23_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7319872.1"
                     /translation="MNELTSIFKALSDETRLRIIKLLERGELCVCDIVAALDMVQPKV
                     SFHLGTLKEAGIIKDRKQGKWIHYSLDEKDLFRRMLMISICEKADGSLVEEDQNRLAG
                     FLRSKKNGPESTKTKGKGCSCGA"
     gene            1528..>2138
                     /locus_tag="VFK23_12105"
     CDS             1528..>2138
                     /locus_tag="VFK23_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET7319873.1"
                     /translation="MIPLTFTQDNRKFYSYASALALITIFYNIVEGLVSVLFGLEDGS
                     MALFGFGVDSFVEVVSGIGIWHMVRRIRNNGSENPDRFEQTALRITGAGFYSLALGLS
                     IAAAVDLYRGHKPETTFWGIVISVVSIGTMWLLIHYKVKVGKRLRSEAILADAACTRA
                     CLQLSVVLLVASAGFELTHFGGIDSLGTFVIAGLCLREGREAL"
BASE COUNT          484 a          562 c          588 g          504 t
ORIGIN      
        1 tcttcgttgt ctccatcatc cgatccttct tcccgcccga gcggaccaaa cgtattctat
       61 cccataaacg ggagtttatt ggaaatattc ttgctgccct cctcggcatc gtaacgccgt
      121 tctgctcatg ctcggctgtt cctctcttta taggttttgt cgaggccgga gtgccactcg
      181 gtgtcacgtt ctccttcctc atctcctctc ctatggttaa cgaggttgct gtggtactcc
      241 tctggggcct gttcggctgg aaggtcgccg tgatctacat gagcgcaggg ttgtctgtcg
      301 cgatcgtcgc ggggttcatc atcggcaagc ttaagctgga gaaatgggtc gaggagtacg
      361 tctacaagat acagtcactc ggagctgcta ttggtacggt cagacagaca ttcaaggacc
      421 gtcttgtata cgcccgcgct aataccattg agatccttca gaaggtctgg ctctacgtgg
      481 tcatcgcaat cggcatcggc gggttcattc acggatttgc gcctgatgat ttcctcgcga
      541 aatatgcagg caacggcaat ccctttgccg tacctctcgc ggtcgcgata ggtgttccgc
      601 tctattccaa cgcggcagga gtgattccca ttgtctacgc gctcatggaa aaggggatga
      661 gcatagggac ggtgcttgcc tttatgatgg cggtaacggc cctgtcgctg cctgagatga
      721 tcatcttgcg aaaggtcatc aaaataccgc ttctggttgt cttcgtatcg atcatgacct
      781 taacaattat tgctgtcggt tatctgttca acgcaatcat ataggaggat taccatggaa
      841 ataaaagtgc tcggaccggg ctgcacgaac tgccataaga tggaagagat ggcgaaacag
      901 gcggtaaagg aactgggaat cagcgccgag gttaccaaga tcacggacat cggagatatt
      961 gtccgccacg gtattttgtc cacgcccggt ctcgttgtaa acggaaaaat aaagcactcg
     1021 ggcaagccgc ttccgagctt ggacaaggtg aaggagctta tcaaatcaga agcgtgatga
     1081 gcttcatcac gcatcgaaga acggtggacc acatgaacga actgacaagt atatttaagg
     1141 cgctctcaga cgaaacacga ctccggatta taaaactcct cgagcggggc gaactgtgcg
     1201 tctgcgacat tgtagcagca ctggacatgg tgcagccgaa ggtgtccttt catctcggca
     1261 ccctgaagga agcgggaatc atcaaagacc gcaagcaggg caaatggatc cattacagcc
     1321 tggacgaaaa ggacctgttc agacgaatgc tcatgatatc gatttgtgaa aaggccgatg
     1381 gctcactggt cgaagaggac cagaatcggc ttgccggttt tctcagatcg aaaaaaaacg
     1441 gtcctgaaag caccaagacg aaaggaaaag gctgctcctg cggagcgtag aaacaccaga
     1501 tctcatgaat acgaggatgg gcagagcatg atccccctga cctttacgca agataacagg
     1561 aagttctatt cttatgcgtc cgcactggcg cttattacca tcttctacaa tatcgtcgaa
     1621 ggcctggtct ccgtcctctt cggccttgaa gacggcagca tggcgctttt cggcttcggc
     1681 gtcgattcct ttgtcgaggt agtctcgggc atcggcatct ggcatatggt gcggaggatc
     1741 aggaacaacg gcagcgaaaa tcccgaccgg ttcgaacaga cggcgctcag gatcaccggg
     1801 gcgggcttct acagccttgc cctcgggctt tctatcgccg cggcagtcga tctctaccgg
     1861 gggcataagc cggagaccac gttctggggc atcgtcatct cggttgtctc catcggtacg
     1921 atgtggctcc tgatccatta caaggtgaaa gtggggaagc ggctcaggtc cgaggccatt
     1981 ctcgccgacg ccgcatgcac ccgcgcctgt ctgcagctct cggttgtctt gctcgttgcg
     2041 agcgccggat ttgaactgac ccatttcggc ggtatcgatt ccctcggaac gtttgtgatc
     2101 gcaggcctct gcctccggga aggcagggag gcactcga
//
LOCUS       DASSLD010000670         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1614557, whole genome shotgun sequence.
ACCESSION   DASSLD010000670 DASSLD010000000
VERSION     DASSLD010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614557"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFK23_12110"
     CDS             complement(<1..267)
                     /locus_tag="VFK23_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase N-terminal
                     domain-containing protein"
                     /protein_id="HET7319874.1"
                     /translation="MKVLVVGGGGREHALVWKIAQSPKVTKIYAAPGNAGIAQLAECA
                     PVKAEDIAGLVAFARSKAIDLTVVGPEGPLSKGIVDEFAKAGLRA"
     gene            complement(343..1218)
                     /gene="larE"
                     /locus_tag="VFK23_12115"
     CDS             complement(343..1218)
                     /gene="larE"
                     /locus_tag="VFK23_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HET7319875.1"
                     /translation="MVIEQKWDHLKALLHDMHGAVLAYSGGVDSSLLLRAAAEVMGAN
                     LIAVTAVSETYPEGELIAAQEIARSLGVTHRIVHTEELASEDFARNPPDRCYHCKKEL
                     FEKLRRLAADEGVSFVMDGSNADDLRDHRPGRKAAAELSVRSPLLEAGFTKAEVRERA
                     RALGLTVWDKPSLACLSSRIPYGTRITPALIRTVGAAEEQLRAFGFGQARVRHHGDIA
                     RIEVMQEDFAKLLNRDIAEKIAARFRELGYIYICLDIEGYRTGSMNQGLRTEGRGTSD
                     DGRERNLRPSSHRAK"
     gene            complement(1357..2163)
                     /locus_tag="VFK23_12120"
     CDS             complement(1357..2163)
                     /locus_tag="VFK23_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET7319876.1"
                     /translation="MVMKREKANYTIQSVSHALDILESFTRTEDELGVTELSKRLALH
                     KNNVFRLLATLENRGYIEQNRDTEHYRLGPKTLQIGSIFIEQRECRRQARPVLEDLAA
                     GSGETAVVAVLRGNKVIYMDGVETDRTVRAVSRVGAIFPAFCTAVGKIQLANLSAADV
                     ERLYPEQVLNALTERGIKTRDALQAELKLTAQRGYALENEETEADVRGIAVPVRDFSK
                     KVIAAIGVIAPASRMTDERLDKGGIISLIQESGRALSTKLGFIAPIARKH"
     gene            complement(2186..2311)
                     /locus_tag="VFK23_12125"
     CDS             complement(2186..2311)
                     /locus_tag="VFK23_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319877.1"
                     /translation="MAAGAAMEMGYTNITIYQAGMPDWLSKGNPVQKGGKPGKLK"
     gene            complement(2324..>2595)
                     /locus_tag="VFK23_12130"
     CDS             complement(2324..>2595)
                     /locus_tag="VFK23_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7319878.1"
                     /translation="KMLLFLLAFMFFGSTSAMAQYPVIDADQLKSRLHGKQKVALIDA
                     RPEDEYQQGHISGAINIRSDQMKEEAARLPKDKTTALIFYCRGEG"
BASE COUNT          479 a          806 c          737 g          573 t
ORIGIN      
        1 ggcgcgcagt cccgctttcg cgaactcgtc cacgatgccc ttggagagag gaccctcggg
       61 gcccacgacc gtcaggtcga tcgccttgga ccgcgcgaag gctacgagtc cggcgatgtc
      121 ttccgccttg accggcgcgc actccgcgag ttgggcgatg ccggcatttc ccggcgccgc
      181 gtagatcttc gtgaccttgg ggctttgcgc aattttccag acgagcgcat gttccctgcc
      241 gccgccgccc actactaaaa ccttcattcc agcctcccta aagactgatc tcgcgaccgt
      301 ttttcatgac gttgtattct ctgatggaca atagaggacc gctcacttcg ctcgatggga
      361 cgacggacgc aaattccttt ctcgtccgtc atcactcgtc cctcgtcctt cggtccttag
      421 tccctgattc atgctccccg tccggtaccc ctcgatgtcc agacagatat agatatagcc
      481 gagttccctg aaccgagccg cgatcttttc cgcaatgtcg cgattaagaa gtttcgcgaa
      541 atcctcctgc atcacttcta tccgcgcaat gtcgccgtgg tgccgcacgc gcgcctggcc
      601 aaacccgaaa gcgcgcaatt gttcctccgc cgcgccgacg gtccggatga gcgccggggt
      661 aatcctggtt ccatagggga tgcgcgagga caggcaggcg agcgagggtt tgtcccagac
      721 cgtcagaccc aaagcacgcg cgcgttcgcg gacctcggcc ttggtaaacc ctgcttccag
      781 gagaggactg cggacggata actccgcagc ggcttttctg cccggacggt ggtccctgag
      841 atcgtcggcg ttcgagccgt ccatgacgaa ggagacccct tcgtccgcgg cgagccgccg
      901 gagcttttca aacagctctt tcttgcagtg gtagcacctg tcaggaggat tgcgcgcaaa
      961 gtcttccgag gccagttcct cggtatgcac gattctgtgc gtcacgccga gtgaacgggc
     1021 aatttcctgt gcggctatga gttctccctc tgggtaggtc tcggaaaccg cggtaaccgc
     1081 gatgaggttg gcgcccatga cctcggctgc ggccctgagc agaagtgagc tgtccacacc
     1141 gccggaataa gcgagcaccg caccgtgcat gtcatggagc agcgctttga gatgatccca
     1201 tttttgctca atcaccatct gcgcgataat agcacagcat gaacaaaagt gtcaataaag
     1261 tcctgctgag gcgaggacag cggggagtga ttagttcgga gtttggagac gaagcagaaa
     1321 cgcaatgccc tgctcaacct cccggctcct cactcctcaa tgtttcctgg caatgggagc
     1381 gatgaagccg agtttcgtgg aaagcgccct gccggactcc tggatcaggc tgatgatccc
     1441 gcccttgtca agccgctcgt cggtcatcct gctcgcaggg gctataacac cgatcgcggc
     1501 gatgaccttc ttcgaaaaat cccttactgg tacggcgatg cccctgacat cggcctcggt
     1561 ctcctcattt tcgagggcat agccccgttg ggctgtcagt ttgagctcgg cctgcagcgc
     1621 atcccgcgtc ttgatgcccc gctccgtcag cgcgttcagg acctgttccg gatacaggcg
     1681 ttcgacatcg gccgccgaga ggttcgcgag ctggatcttg cccaccgcgg tgcaaaacgc
     1741 agggaagatg gcgccgacgc gcgagacggc gcgaacggta cggtccgtct ccacgccgtc
     1801 catgtagatg accttgttcc cgcgaaggac ggcgaccacc gcagtctcgc cggatccggc
     1861 agcgaggtcc tcgagcacgg gccgcgcctg gcgccggcat tcccgctgct cgatgaagat
     1921 cgagccgatc tggagcgttt taggcccgag ccggtagtgc tccgtgtccc tgttctgttc
     1981 gatgtaaccg cggttctcaa gggtggccag gagacggaag acgttgttct tatggagggc
     2041 caggcgcttc gacagctccg tcacgccgag ttcgtcctcg gtgcgcgtga aggattcgag
     2101 aatatcgagc gcatgcgaca cggactggat cgtatagttt gctttttccc gtttcatgac
     2161 catgagttct gccggttcct ttcagttact tcagtttccc cggtttgccg cccttctgca
     2221 ccgggttgcc cttcgacagc cagtcaggca tgcctgcctg gtaaatcgta atattcgtat
     2281 accccatctc catggccgca cctgcggcca tacggctgag acctcagccc tctcccctgc
     2341 agtaaaagat gagggccgtc gttttgtcct tgggcagtcg cgcggcctcc tccttcatct
     2401 ggtccgaccg gatgttgatc gcgccggaga tatgtccctg ctgatattca tcttcgggtc
     2461 ttgcatcaat gagggccact ttctgctttc catgcagacg ggacttgagc tggtccgcgt
     2521 cgataacggg atattgggcc atggccgatg ttgaaccaaa gaacataaat gccaataaaa
     2581 acaataacat tttcg
//
LOCUS       DASSLD010000671         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_269345,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000671 DASSLD010000000
VERSION     DASSLD010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269345"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VFK23_12135"
     CDS             complement(<1..358)
                     /locus_tag="VFK23_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319879.1"
                     /translation="MRNGVKKKAVYCLLALVVFLMGAGKVDPIRDERTALRAERRALL
                     QKIEKLKHEQDYLLFQKTMYENDSKYLVIDIGKKTGHLRYRNRVLKDFKFQTSKNFPV
                     RSLKPGMLTLTEKIEGK"
     gene            complement(379..1041)
                     /locus_tag="VFK23_12140"
     CDS             complement(379..1041)
                     /locus_tag="VFK23_12140"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET7319880.1"
                     /translation="MRNRKHKALIWTATVFIAALTFSLLSHLYSWAGSVPSTPSLDEL
                     RAEVQALRQEAAMLEEENRGLRKVLADQEDGELYVVVDTESNRLTLRQGETVLHTAVA
                     GTGSRRFIEAETGANWFFETPIGSFTVLGKERNPVWIRPDWSYVEENMPIPPPRDPDR
                     IVRGVLGKYALLLGNGYKIHGTKWTKLLGTHFTHGCVSLGDKDLEMVYNSVKIGTKVY
                     LF"
     gene            complement(1031..>2156)
                     /locus_tag="VFK23_12145"
     CDS             complement(1031..>2156)
                     /locus_tag="VFK23_12145"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7319881.1"
                     /translation="RMNRILEIDEEDFIAVVEPGVVTGEFQKAVEERGLFYPPDPASR
                     QFCTLGGNVAECAGGLRAVKYGVTKDYVTGLEVVLPTGEIIRTGARTVKSVAGYDLTK
                     LIVGSEGTLGGVTNITVRLLPLPESVRTLSACFSSVDDAARAATNILAGRVIPRALEF
                     IDQTALRAVENYLKEDFSGGAAAMLLAEVDGTSASTLVEIDRIAEIMQKAGAVRVQAA
                     ASEAERERLWKARRSISPALYTIKPKKINEDLVVPRSRIADLLRATGEIGRKYGLMVV
                     SFGHAGDGNIHTNVLVDDADLPRAGDAVKDIFAAALRLGGSITGEHGIGLSKAAYLPM
                     ELEADALAVMRRIKKALDPNNILNPGKIFLNAEFGVRNAE"
BASE COUNT          394 a          678 c          602 g          482 t
ORIGIN      
        1 tcttcccctc tatcttttcg gtgagcgtca gcatccccgg cttgagcgag cggaccggaa
       61 aattcttcga ggtctggaac ttgaagtcct tcagcacacg gttcctgtac ctgagatgtc
      121 ctgttttttt cccaatatca attacgaggt atttcgagtc gttctcgtac atcgttttct
      181 ggaaaagaag ataatcctgt tcgtgcttga gcttctcgat cttctgaagg agcgcgcgcc
      241 gctcggccct gagcgcggtc cgctcgtccc ggatcgggtc gactttgcct gcacccatga
      301 gaaaaacgac gagtgccaga agacaataga cggccttctt cttaacacca ttccgcattc
      361 cgcattccga attccacatt agaatagata caccttcgtg ccgatcttca ccgagttgta
      421 aaccatttcg agatccttgt ccccgaggga gacacatccg tgcgtgaagt gcgtgcccag
      481 gagtttggtc catttcgtcc catggatctt gtatccgttt ccgagcaaaa gcgcatactt
      541 tccgagcaca ccgcgaacga tgcggtcggg gtcgcgcgga ggcgggatgg gcatgttctc
      601 ttccacatac gaccagtccg gcctgatcca gacggggttc cgctcttttc cgagcacggt
      661 aaacgaccct atgggcgtct cgaagaacca gttcgctccg gtctccgctt caataaagcg
      721 gcggcttccg gtcccggcga cggcggtatg gagaacggtc tctccctggc gcagggtcag
      781 ccggttgctc tcggtgtcca ctacgacgta cagctctccg tcctcctggt ccgccaggac
      841 cttccggagg ccccggttct cttcttcgag catagccgcc tcctgccgca gtgcctggac
      901 ttcagcacga agctcatcga gcgaaggagt tgagggaacg cttcccgccc atgagtacag
      961 atgtgacaac agcgaaaaag tcagggctgc gatgaaaacg gtcgcggtcc agatcaatgc
     1021 tttatgtttt ctattccgca ttccgcactc cgaattccgc attcaaaaaa atctttcccg
     1081 ggttcaggat attgttcgga tcgagagcct ttttgattcg tctcatgacg gcgagcgcat
     1141 ccgcctccag ttccatcggg aggtaggcgg cttttgaaag gccgatccca tgttcgcccg
     1201 tgatgctccc gccgaggcgg agcgccgcgg cgaagatgtc cttgacagca tccccggcgc
     1261 gcggcaggtc agcgtcatcg accaggacgt tcgtatggat attgccgtcg ccggcatgtc
     1321 cgaagctcac gaccatgaga ccgtatttcc ggcctatctc accggttgcg cgcagaaggt
     1381 ccgcgatcct gctccggggg accacgaggt cctcgtttat ttttttcggc ttgatggtat
     1441 agagggccgg agagatcgag cgccgggcct tccagagcct ttcgcgctca gcctcggaag
     1501 ccgccgcctg cacccggacg gctcccgcct tttgcataat ctccgcgatc cggtcgatct
     1561 cgaccagggt ggatgcggat gtgccgtcca cctccgccag gagcatggcg gccgctcctc
     1621 cggagaagtc ctccttgaga tagttctcca ccgcgcgcag ggcagtctgg tcgataaatt
     1681 cgagggcacg cgggatgacc cttcccgcga ggatattcgt cgccgcccgc gcagcgtcat
     1741 cgacgctgct gaagcacgcc gacagagtcc tcacgctctc gggcaggggc agcagcctga
     1801 ccgtgatgtt cgtgaccccg cccagggtgc cttcggagcc gacaatgagc ttcgtgaggt
     1861 cgtatcccgc gacgctcttc acggtcctcg cgccggtcct gatgatttcg cccgtgggca
     1921 ggacgacttc caggcccgtt acgtagtcct tcgtgacgcc atactttacg gcgcgcagcc
     1981 cgccggcgca ttcggcgacg tttccgccga gcgtacagaa ctgcctgctc gccggatcgg
     2041 gaggatagaa gaggccccgc tcttcgacgg ctttctgaaa ctcgccggtt accacacccg
     2101 gctcaaccac ggcgatgaaa tcttcctcgt cgatctcgag gatcctgttc atccgc
//
LOCUS       DASSLD010000672         4141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_995316,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000672 DASSLD010000000
VERSION     DASSLD010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995316"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFK23_12150"
     CDS             <1..598
                     /locus_tag="VFK23_12150"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET7319882.1"
                     /translation="NAVGNRGFSFKDSASQAAPKLKDLLNHDQSLVRQEAAYALGNIG
                     FAHTDLVRDAVPRLIRMLEGGDDDEREAAAHALGKVGINAPELIRGILPKLVEVLEFS
                     KEATCGGVLFALGAIGLHYPGEVAALVPKLITYLDSVHPNAVKNAVAALGNIGSTNPE
                     LVRDALPKIRLILETSTDIDIKMNAKVALGRMGDPAAM"
     gene            707..1381
                     /gene="scpB"
                     /locus_tag="VFK23_12155"
     CDS             707..1381
                     /gene="scpB"
                     /locus_tag="VFK23_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET7319883.1"
                     /translation="MDDQQLKSAIEAILFVAGNPLSVERLTGLFEEEDAGRIETQLAS
                     LAQEYDSRGAGIMLAEVAGGYQLVTRQENAPWVRKFKSVKVSSKLSRPALETLAIVAY
                     KQPITRTEVEAIRGVNIGGIMRNLMERRLVKIVGKKDVPGKPMMYGTTPEFLQYFGMK
                     DLSSLPTLKEFQELETAEEVMEEMPVDGAGASEPGADLRQDDAAPGEAGEAEPLTALT
                     EENASE"
     gene            1378..2325
                     /locus_tag="VFK23_12160"
     CDS             1378..2325
                     /locus_tag="VFK23_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HET7319884.1"
                     /translation="MIELSDFLRKKEIWAVGGGKGGIGKSLITGNMGIHLARLNKKVL
                     LVDADLGGANLHTTLGIGVPEMTLSDFLSRRVERIQDVIIGTSIPNLSLISGAQDFLD
                     AANPNFAQKARLLRNLDALDTDYILLDLGAGTSFNILDFFLFSDHGMLVVLPEPTAIE
                     NAYRFIKSAFYRRFKKVVAHHKDVREIIDTAMDQKNAAGLRTPHDLIERIKTLDRGIG
                     EVLELEMLKFKPKLIVNQVRTKNDVQIGFSMKSACHRYFGINVEYLGYVDYDDCVWQS
                     IRSKRPLAVEYPFSRPSKGIERIVNNVLRKEQLAVSVLY"
     gene            2365..3048
                     /locus_tag="VFK23_12165"
     CDS             2365..3048
                     /locus_tag="VFK23_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7319885.1"
                     /translation="MKKIENLDYYDLLEVSPSATIQEIHKAYERLKKIYDPNSIALYS
                     LFTPEETAALQIKLEEAYRTLILEDKRRQYDAALKNLGDVSEPAAPDSPQTPAPHPEQ
                     SSLPLPADADQFTAPGEAPRPPDPAPARTTGEIKPPPEFTGEFTGPAIKTLREQIGYD
                     LRTVAGMTKVSIRYLEMIEDENFGKLPARTYIRGFLMLYAKTLGYDAEKLAGDYLKRY
                     DEAMKPTKQ"
     gene            complement(3070..>4141)
                     /locus_tag="VFK23_12170"
     CDS             complement(3070..>4141)
                     /locus_tag="VFK23_12170"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET7319886.1"
                     /translation="ALRAEVREKYEGGLVGNTAAMQEIYKTIGRVAESDATVLIHGES
                     GTGKELVARAIHYHSKRAGRPFVAVNSAAIPSELLESELFGHEKGSFTGAVARKIGKF
                     EAASGGTLFLDEIGDMNLPLQGKLLRVLQEKEFERVGGTEPIKTDVRVLAATHHNLEK
                     MVREKRFREDLFYRLNVIQINIPPLRKRKDDIVALAEFFMQKYQGSDAKKKILTAETL
                     KILRAYDWPGNVRELENAVQRAITLSQGDKIFPDALPPQIFRPGQGAHLSFENFLEEK
                     LADLVERMGGLDRGDIYSLVLHRVEKPLITLVLRKTEGNQVRAAHLLGINRNTLRKKI
                     KELGIKGPFNGEEEGEKAAEDQ"
BASE COUNT          853 a         1285 c         1248 g          755 t
ORIGIN      
        1 gaacgccgtc gggaaccgcg gattcagctt caaggactcg gcttcacagg ccgcgcccaa
       61 gctcaaggac ctcctgaacc acgaccagag cctggtccgc caggaagcgg cctatgctct
      121 gggaaacatc ggcttcgccc atacggacct ggtgagggac gccgttcccc gtcttatccg
      181 gatgctcgag ggaggagatg acgacgagcg cgaggccgcg gcccacgccc tgggcaaggt
      241 cggcatcaac gcgcccgaac tgatccgcgg gatcctgccg aagctcgtcg aggtgctgga
      301 gttctcgaag gaggcgacct gcggcggcgt gctttttgcg ctgggcgcca taggactgca
      361 ctaccccggg gaagtggcgg ctctggtgcc gaaactgatc acgtacctgg acagcgtcca
      421 tcccaatgcg gtcaagaacg ccgtagcggc gcttggcaat atcggcagta cgaaccccga
      481 gctggtacgg gacgcgcttc cgaagatccg gctcatcctc gagacctcga cggacatcga
      541 catcaagatg aatgccaagg tggcactcgg ccggatgggt gatcctgcgg cgatgtgagt
      601 gatcgcaaat cgcatagagc atagcgcaaa gcgcatggcg gaagggggat tatgttttac
      661 gctatgcgcc ctgcgctatg ccctctgcgt atttaaggag taatacgtgg acgatcaaca
      721 actgaaatca gcgatcgagg cgatcctgtt cgtggctggc aatcctttgt ccgtggaaag
      781 gctgacaggc ctgttcgagg aggaggacgc cgggcggatc gagacgcaac tcgcctcgct
      841 cgcgcaagaa tacgacagcc ggggcgcggg catcatgctt gccgaagtgg caggcgggta
      901 ccagctcgtg acgcgacagg agaacgcccc gtgggtccgg aagttcaagt ccgtcaaggt
      961 ctcctcaaaa ctttcccggc ccgcgcttga aaccctggcc atcgtggctt acaagcagcc
     1021 cattacgcgg actgaagtgg aggccattcg cggcgtgaac atcggcggga tcatgcggaa
     1081 cctcatggag cgcaggctcg tgaagatcgt gggaaagaag gacgtgccgg gcaagcccat
     1141 gatgtacggt acgacgccgg aatttctcca atatttcggc atgaaggacc tctcgtccct
     1201 gccgacgctc aaggaattcc aggaactcga gactgccgag gaggtcatgg aggaaatgcc
     1261 ggtcgacggc gcaggcgcct cggagccggg ggccgatctg cggcaggatg acgcagcccc
     1321 cggggaagcg ggagaagccg aacctctaac cgccctgacc gaggagaatg cttcggaatg
     1381 attgaactct cggatttcct ccgcaaaaaa gagatctggg ccgtcggcgg gggcaaaggc
     1441 ggcatcggca aaagcctgat taccggcaac atgggcatcc atctcgcacg tctcaacaag
     1501 aaagtgcttc tcgtcgatgc cgacctgggc ggcgcgaacc tccataccac cctcggtatc
     1561 ggcgtgccgg agatgacgct ttccgatttt ttgagccggc gggtggaacg catccaggac
     1621 gtgatcatcg ggacgagcat tccgaacctg agcctcatca gcggcgccca ggacttcctc
     1681 gacgccgcca accccaattt cgcgcagaag gcgcggctgc tcagaaatct tgacgccttg
     1741 gacaccgatt atatactcct cgacctcggc gcgggcacgt cgttcaacat cctcgacttc
     1801 ttcctcttct ccgaccacgg catgctggtg gtcctgcccg agcccacggc gatcgaaaac
     1861 gcttaccggt tcatcaagag cgcattctat cgcaggttca agaaggtcgt ggcgcaccac
     1921 aaggacgtca gggaaatcat cgacacggct atggaccaga aaaacgccgc agggctccgc
     1981 acgccccatg acctcatcga gcgcatcaaa acgttggacc ggggaatcgg cgaggtcctc
     2041 gaactcgaaa tgctcaagtt caagcccaag ctgatcgtga atcaggtgag gaccaagaac
     2101 gacgtccaga tcgggttctc gatgaagagt gcctgccacc ggtacttcgg catcaacgtg
     2161 gaatacctgg gatacgtcga ctacgacgac tgcgtctggc agtccatccg gagcaagcgg
     2221 cccctcgccg tcgaataccc cttctcgcgg ccgtcgaagg gcatcgaacg catcgtcaac
     2281 aacgtgctcc ggaaggagca gctcgcggtc agcgtgctgt actagcccgg ggccgcctgc
     2341 aggccttgtg gggtggtcgc attgatgaaa aaaatagaaa atctggatta ctacgatctg
     2401 ctggaagtga gtccttcggc cacgatccag gagattcaca aggcctatga acggctcaaa
     2461 aagatctacg atccgaattc aatcgccctc tactcgctct ttactcccga agagaccgcc
     2521 gcactgcaga taaagcttga agaagcatac cgcacgctca tccttgagga caaacgaaga
     2581 caatacgacg ccgcgttgaa gaacctgggc gacgtgtccg aaccggcggc tcccgactcg
     2641 ccccagaccc ccgccccgca tccggagcag tcgtctttgc cgctccctgc agacgccgat
     2701 cagttcaccg ctcccgggga agcgccccgg ccccccgacc cggcgccggc ccggacgacc
     2761 ggggagatca aaccgccccc cgagttcacc ggggaattta ccgggcccgc catcaagacc
     2821 cttcgcgaac aaatcggcta tgacctgcgg accgtcgcgg gcatgacgaa ggtaagtatc
     2881 cggtacctgg aaatgatcga ggatgaaaat ttcgggaaac tgccggcgcg gacctatatc
     2941 cggggcttcc ttatgctcta tgcaaagacc ctcggatacg acgcagaaaa gctcgcgggt
     3001 gactacctca agcgctacga cgaagcgatg aaaccgacga aacaatagct gcagcgaaaa
     3061 tcgcggcgat cactggtcct ctgccgcctt ctctccctcc tcctcaccgt tgaaaggccc
     3121 cttgatcccc aattccttga tctttttccg gagcgtgttt cgattgatgc cgagaaggtg
     3181 ggcggcacgg acctggttgc cctcggtctt tctgaggacc agggtgatca gcggtttttc
     3241 cacccggtgg agcacgagcg agtagatgtc cccccgatcg agccccccca tgcgctccac
     3301 gaggtcggcc agcttttcct cgaggaaatt ttcgaacgac aggtgcgcgc cctggcccgg
     3361 cctgaagatc tgcggcggca gcgcgtcggg aaatatcttg tcgccctggg agagggtaat
     3421 ggcgcgctga acggcgtttt caagctcgcg cacattcccc ggccagtcat aggcgcggag
     3481 gatcttgagc gtctcggccg tcaggatctt tttcttcgcg tcgctgccct ggtatttctg
     3541 catgaagaac tcggcgagcg cgacgatgtc gtccttgcgc ttgcggagcg gcgggatgtt
     3601 gatctggatc acattgaggc ggtagaacag gtcctcgcgg aaacgcttct cgcgcaccat
     3661 cttttcgagg ttgtggtggg tcgccgccag gacgcgcacg tcggtcttga tcggctcggt
     3721 gccgcccacg cgctcgaact ccttttcctg cagcacgcgc aggagcttgc cctggagcgg
     3781 caggttcatg tctccgatct catccaggaa gagcgttccg cccgacgcag cctcgaactt
     3841 tccgatcttg cgggcgaccg cgccggtgaa ggagcccttc tcgtggccga agagctcgct
     3901 ctcgaggagt tccgagggaa tggccgcgga gttcacggcc acgaacggcc tgcccgcccg
     3961 cttgctgtga taatgaatgg cccgtgccac aagctccttg cccgtgccgc tctcgccgtg
     4021 gatcaggacc gtggcgtcgc tttcggcgac gcggccgatg gtcttgtaga tctcctgcat
     4081 ggccgccgtg ttgccgacga gcccgccctc gtacttctcc cgcacctcgg cccggagcgc
     4141 g
//
LOCUS       DASSLD010000673         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_725850,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000673 DASSLD010000000
VERSION     DASSLD010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725850"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /gene="htpG"
                     /locus_tag="VFK23_12175"
     CDS             <1..1083
                     /gene="htpG"
                     /locus_tag="VFK23_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HET7319887.1"
                     /translation="SEFTALLYVPSKAPYGILYRDFKIRPTLYVKRVQIMDHCEQLIP
                     PYLRFVRGVVDSSDLPLNVSREILQSNRHVEVINRNITKKVLDTLADMKKNSFEAYVV
                     FHQEFGRVLKEGIHYDFSRKEQIADLLLFRSTKTESGKYTTLDDYVKNMPIAQAEIYY
                     ITGRPDENVIESPYLEAFREKGYEVLIMTDDIDDIIMLGLGEYKGKKIKSVVKGDISL
                     DKDNQAEKKKAEGKFEKLIARIKEQLKDEVKDVRLSGRLKDSACCLVSDEGGLDPQME
                     KLLKSMGQDVPAGKRVLEINPDHQVFSAMNDLIEKGGQDELVKEYIGLLYDQALLLEG
                     SKVKEPAAFAKAMARLMSENARRHAA"
     gene            1314..1625
                     /locus_tag="VFK23_12180"
     CDS             1314..1625
                     /locus_tag="VFK23_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319888.1"
                     /translation="MKTVGVLIAVVFALTLGLAASMAWAEEVRFDNGVTFSDLGSAPD
                     CESVIGVGAGGLINEEPGEVMENGITFFEPIMPGTVAIGRCAGALAPSPAVYNGVTVF
                     E"
     gene            complement(1759..>2191)
                     /locus_tag="VFK23_12185"
     CDS             complement(1759..>2191)
                     /locus_tag="VFK23_12185"
                     /inference="COORDINATES: protein motif:HMM:NF017816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HET7319889.1"
                     /translation="RKGIAWSDGKKYDSATSWHVTWKYDYNRTPDACSADSFRVFVEL
                     TFLYPRWVPGGDTTRDLTDRWEAYMKNLVTHENGHRDLAVEAAAELARAVAELPPSPT
                     CADVDREVRVLSRVRMDMLNDDEKEYDTATNHGYTQGAVFR"
BASE COUNT          522 a          572 c          635 g          462 t
ORIGIN      
        1 tcggagttta ctgccctcct ctacgttccg tcgaaggcgc cctatggcat cctctaccgt
       61 gacttcaaga tcagaccgac cctgtacgtc aagcgggtcc agatcatgga ccactgcgaa
      121 cagctcattc ccccctacct gcggttcgtc aggggagtgg tggactcttc cgacctgccg
      181 ctgaacgtat cgcgggagat cctccaatcc aacaggcatg tggaggtcat caacaggaac
      241 atcaccaaga aggtgctcga cacccttgcg gacatgaaaa agaactcctt tgaggcctat
      301 gtcgtgtttc atcaggagtt cggcagggtc ctgaaggagg gcatccatta cgatttttcc
      361 cggaaggaac agatcgccga cctgctcctg ttccgctcca ctaagacgga gtctggcaag
      421 tacacgacgc ttgacgacta cgtgaagaac atgccgatcg cccaggcaga gatctactat
      481 atcacgggca ggcccgatga aaatgtcatc gagtcgccct atctcgaggc gttccgtgaa
      541 aagggctacg aggtcctgat catgaccgat gacatcgatg atatcatcat gctcggtctc
      601 ggagagtaca agggcaagaa gatcaaatcg gtcgtcaagg gagacatcag cctcgataag
      661 gacaatcagg cggagaagaa aaaggcggag ggaaaattcg agaagctcat cgccaggatc
      721 aaggagcagc tcaaggacga ggtgaaggac gtgcgtcttt ccggcaggct caaggactcg
      781 gcctgctgcc tcgtctccga cgagggcgga ctcgatccac agatggagaa gctgctgaag
      841 tccatgggac aggacgtgcc cgccgggaag agggtcctgg aaatcaaccc ggaccatcag
      901 gtcttctcgg ccatgaacga cctcatcgaa aaaggcggcc aggacgagct ggtgaaggaa
      961 tatatcgggc tcctgtacga ccaggcgctc ctgctcgaag gctccaaggt caaggaaccg
     1021 gccgcgttcg caaaagctat ggcaaggctc atgtcggaga atgccaggcg gcacgcggcg
     1081 tagcatcggg tcctgttgca ggatcgttct atgaattcgt tgcagaccgt tcctgcaggt
     1141 caaacagaag aagcagcggt gcgattattt gccgcacctg aacaaaaacg agacgccatt
     1201 taatagaaag accattagga accaaactgt tatttactgt cgtcttgaca atcatcagtg
     1261 cggtgttacg attaaagaag ggaggcttac aacaagaaaa gaggcgatgc agtatgaaga
     1321 ccgttggtgt tctgatcgcg gtagtatttg ctcttacttt gggcttggcc gcaagcatgg
     1381 catgggctga ggaagtcagg tttgacaatg gtgtcacgtt ttctgacctg ggttcggctc
     1441 cggattgtga atccgttatc ggcgtgggcg caggaggcct gatcaacgag gagccgggcg
     1501 aggtgatgga aaacgggatt acgttctttg agccgattat gccggggaca gttgcaatag
     1561 gcaggtgtgc aggagcatta gcgccgagtc cagcagtgta caatggagtg accgtttttg
     1621 agtaatgagg gcagacgcgg gtacgtcact tctgatcctc gcagttgcaa atgttgagag
     1681 cctcccttag agttgagaaa tgcatccgca atcgtgaagc aggcagagta gcctgctttt
     1741 ttgttgttca ggcagcggtc aacggaacac agccccctgt gtgtaaccgt ggttggtcgc
     1801 cgtatcgtac tccttctcat catcattcaa catatccatc cgcacgcggg acagcacccg
     1861 cacttcacgg tcgacgtcgg cacaggtggg tgaagggggc aattctgcca cagcgcgggc
     1921 aagttcagcc gccgcctcca cagcgaggtc ccgatggccg ttttcgtgag tgacaaggtt
     1981 cttcatatag gcttcccatc tatccgtgag gtcccgggtt gtgtcgccgc ccggcaccca
     2041 tctcggatag agaaaggtta gttcgacgaa cacccggaag gagtcggctg aacaggcatc
     2101 cggggtgcgg ttataatcgt acttccacgt cacatgccag ctcgtcgcag aatcgtattt
     2161 tttcccgtcg ctccacgcaa ttcccttccg c
//
LOCUS       DASSLD010000674         2999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_636612,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000674 DASSLD010000000
VERSION     DASSLD010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636612"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..414)
                     /gene="panD"
                     /locus_tag="VFK23_12190"
     CDS             complement(61..414)
                     /gene="panD"
                     /locus_tag="VFK23_12190"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HET7319890.1"
                     /translation="MFRLMLRSKIHRATVTDANLEYEGSLTVDPVLLEAAGILPYELV
                     HVSNLNNGERFETYVIPGRRGSGEMVLNGPTARRGVKSDKIIVFCYEYFNEDELRTFK
                     PKIVLVDENNRVREK"
     gene            complement(525..812)
                     /gene="gatC"
                     /locus_tag="VFK23_12195"
     CDS             complement(525..812)
                     /gene="gatC"
                     /locus_tag="VFK23_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020618885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET7319891.1"
                     /translation="MKISKQEVEHVAKLARLELTDQEKEKLTDQLSNILTYVEKLNEL
                     DTKGVEPTAHVLDINNVMRNDEPGESLTQERALANAPDKAAGHYKVPKIIE"
     gene            complement(856..>2999)
                     /locus_tag="VFK23_12200"
     CDS             complement(856..>2999)
                     /locus_tag="VFK23_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET7319892.1"
                     /translation="ITCRIAHLIREQGVDPGSILAVTFTNKAATEMNERLERMLGITL
                     GRGIDHGGLWISTFHSACVRILRRHIDRIGYKRSFVIYDETDRSSLIKACVTDLRVDA
                     ERYLPRAIAAKISSLKNNLTGVEAFVRTNSGFGFDDAVARVYALYEEKLRESDGLDFD
                     DLLLQTVRLFERDRDTLGYYQNIFHHILIDEYQDTNHAQYRLVRLLTAERQNLCVVGD
                     DDQSIYKFRGADIANILNFEKDYPAAKVIKLEQNYRSTQNILGAAGAVVARNLGRKPK
                     ELWTERRGGDRIICYKAFDEKDEARFICRSIQKEVDEGRSLRQIAVLYRTNAQSRALE
                     DALRNWGTPYRIFGGLRFYDRKEIKDVIAYLRVLQNPSDIVSLRRIINVPARGIGDTT
                     IEKLEEAAAIARIGLYQTALEAEHTGIASAAKKKLKEFTGMMERMRADLGRMTLTELV
                     RRVIQDSGYGAALEREKSVESRIRLENLNELLTATEDFQEQNRDASPAAFLDQVALIT
                     DAEQQSSAEGRRGGADAVTLMTLHNAKGLEFAVVFMAGMEEGLFPHSRSAESEEELEE
                     ERRLCYVGITRAKERLILTHAAERRLYGYPQANLVSRFVQEIPDGAMDWEGTDMPAAI
                     SFRDRATWERPSSDADRKGTPERFSAAATAEKPGPADRYYKGAVVRHAKFGLGTVQRS
                     EGSGDDRKISVSFPGHGVKTLALKYANLETI"
BASE COUNT          466 a          959 c          869 g          705 t
ORIGIN      
        1 tggtctcgcc tttcacgagc atgtcgtgca gttcgtggat agtccgctcg tataactcca
       61 ttatttctcc ctcacccggt tgttttcatc caccagaacg attttcggtt tgaaggtcct
      121 gagctcgtcc tcattaaagt actcgtagca gaagacgatg atcttgtccg acttgactcc
      181 ccgccgggca gtcgggccgt tcaacaccat ctcgcccgag ccgcgcctgc ccgggatgac
      241 gtaagtctcg aaccgttccc cgttgttcag gttcgagaca tggaccagct catagggcag
      301 aatgcccgcc gcttcgagca gcacgggatc gacggtcagg ctcccctcgt actcgaggtt
      361 cgcgtccgtc acggtcgcgc ggtgtatctt cgatcgcagc atcagtctga acatgaatct
      421 cctttgaaca cgatggtgaa cgatgtgtga ggagtgaggt gatgctctcc tgactcctga
      481 ctcctgactc ctgactcctg actcctgact cctgactcct gactctattc gatgatcttc
      541 ggcaccttgt aatggcctgc cgccttgtcc ggcgcattgg caagggcgcg ttcctgcgtc
      601 aggctctctc cgggttcgtc gttgcgcatg acgttgttga tgtccagcac gtgggcggtg
      661 ggttccaccc ctttggtatc gagctcgttc agcttctcga cgtaggtgag aatgttcgag
      721 agctggtcgg tcagtttttc cttctcctgg tccgtcagct ccagccgcgc gagcttcgct
      781 acgtgttcga cttcctgttt tgatattttc atgcctgact ccgaactcct gaattctgac
      841 tggtgtcttc tgaatttata tcgtttccag gttcgcgtac ttcaatgcga gcgtcttgac
      901 gccgtgaccg gggaagctga cgctgatctt gcggtcgtcc cccgacccct cggaccgctg
      961 gactgttcct aatccgaact tcgcatggcg caccacggcg cccttgtagt accggtcagc
     1021 ggggccgggc ttctccgccg tggcggccgc gctgaagcgc tccggcgtcc ctttccggtc
     1081 cgcgtcggat gacggccgtt cccacgttgc ccggtcccgg aaggagatgg ccgccggcat
     1141 gtccgtccct tcccagtcca tggccccgtc cggaatctcc tgcacgaacc gggagacgag
     1201 gttcgcctgc ggatatccgt aaagcctgcg ttccgccgcg tgggtgagga tgagccgctc
     1261 cttggcccgc gtgatcccca catagcagag ccttctctcc tcctccagtt cttcctcgct
     1321 ctccgcggag cgcgaatggg gaaagaggcc ctcctccatg cctgccatga agaccacggc
     1381 gaactccagc cctttggcgt tatggagcgt catcagggtg accgcgtccg ccccgcccct
     1441 cctgccttcg gccgagctct gctgctccgc gtccgtaatg agcgcgacct ggtccaggaa
     1501 cgcggcgggc gacgcgtcgc ggttctgttc ctggaagtcc tcggtcgcgg tcaaaagctc
     1561 gttcaggttt tcgagccgta tccgcgactc cacgctcttt tcccgttcga gtgcggcgcc
     1621 gtacccggaa tcctggatga cgcgccgcac cagctccgtg agggtcatgc gtccgagatc
     1681 ggcccgcatc cgctccatca tgcccgtgaa ttccttgagc ttctttttcg cggcggacgc
     1741 gatgccggta tgttctgcct cgagggccgt ttgatagaga ccgatccgcg cgatagcggc
     1801 cgcttcttcc agtttttcta tcgtcgtatc gccgatgccg cgcgccggaa cgttgatgat
     1861 cctgcgcagg cttacgatgt cggacgggtt ttgcagcacc ctgaggtatg caatgacgtc
     1921 cttgatctcc ttgcggtcgt agaaccggag gcctccgaag atccggtacg gcgtccccca
     1981 gttccggagc gcgtcttcca gggcgcggga ctgggcgttg gtgcggtaga gcaccgcgat
     2041 ctgcctgagg ctcctgccct cgtcgacttc cttctggatg gacctgcaga tgaaccgcgc
     2101 ctcgtccttc tcgtcgaagg ccttgtagca aattatcctg tcgccgccgc gcctctcggt
     2161 ccagagttcc ttcggttttc tcccgaggtt tcgcgcgacc accgctcccg ccgcaccgag
     2221 tatattttgc gtcgaacggt agttctgctc cagcttgatg accttggcag cgggatagtc
     2281 cttctcgaag ttcaggatgt tcgcaatgtc cgccccgcgg aacttataga tgctctggtc
     2341 gtcgtcgccc accacgcaga ggttctgcct ctcagccgtc agcagccgca cgaggcggta
     2401 ctgggcgtgg ttcgtgtcct ggtactcgtc gatcaggatg tgatgaaaga tgttctggta
     2461 gtacccgagc gtatcgcggt cccgctcgaa gagcctcacc gtctgcagga gcaggtcgtc
     2521 aaaatcgagt ccgtccgatt ccctgagctt ttcctcgtac agggcgtaga cgcgggcgac
     2581 cgcgtcgtca aaaccgaagc cggagtttgt cctgacgaac gcttcaacac ccgtcaggtt
     2641 gttcttgagg ctgctgatct tcgcagcgat cgcccgcgga aggtagcgtt cggcgtcgac
     2701 cctgaggtcc gtcacgcagg ccttgatgag cgaggagcgg tctgtctcgt cgtagatcac
     2761 gaaggagcgc ttgtacccga tgcggtcgat gtgccgacgc aggatcctga cgcaggccga
     2821 gtggaacgtg ctgatccaca gccctccgtg gtcgataccc cggccgagcg taatacccag
     2881 catccgctcg agccgctcat tcatctccgt cgcggccttg ttcgtaaagg tgaccgcgag
     2941 gatgctgccg gggtcgacgc cctgctcgcg aatgaggtgc gcgatcctgc acgtgatca
//
LOCUS       DASSLD010000675         3302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_521992,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000675 DASSLD010000000
VERSION     DASSLD010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521992"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="VFK23_12205"
     CDS             complement(<1..1380)
                     /locus_tag="VFK23_12205"
                     /inference="COORDINATES: protein motif:HMM:NF036965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HET7319893.1"
                     /translation="MSKNHKVIFQPSGRRGEVAEGKTILEAAQSLGVDIEALCGNKKV
                     CGKCKVRIEEGYVEKDNIDSGMAHIYPAVPSPDEMKHIKPQDGPGIRLACQCEIRGDV
                     KVFVPERSRAGKQVVRKAAKELTIKLDPAVKKYNVDLVAPTLHDMTTGDWERVLMFLE
                     ADYGLKGLTFDYLVLKNLQDVLRKGGWKATMTVWMDKEIIKAEPGFVEESYGLAVDIG
                     TTTCVGYLTDLNTGKVVNTESMMNPQVPYGEDVMSRITYAMSNPGVGLETMQKAIIQG
                     LNEIIERVVTEIRKDGPNPGHVIDDLTIVFNTAMHHIFLGLNPEYIGRSPFIPAVQTS
                     LDIKARDVGLRINPAAYIHVLPIEAGFVGADNVGVLIAEEPYNQDEKVLIIDIGTNGE
                     LLMGNRDKVCSTSCATGPAFEGAQIKFGMRAAPGALETVQIDPETKEPRYKVIGKADW
                     HTHIAKVDAK"
     gene            complement(1441..2109)
                     /locus_tag="VFK23_12210"
     CDS             complement(1441..2109)
                     /locus_tag="VFK23_12210"
                     /inference="COORDINATES: protein motif:HMM:NF024875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HET7319894.1"
                     /translation="MNAELRKELEGLGADVVGFAGLKGYMNGEIAHLEQAVSIGVARR
                     LNEDTLSILMKLQKRAVRFLKSRGYRSLAIPPDSDRKKGTFVSKLYSLFNHKMAATSA
                     GLGWIGRNGLLISPDYGPRLSLVTVLTDAPFEPDEPVEHSLCGDCSLCAAYCPSKAIT
                     GAEWSRSFPFVELVRLDVCRSHKKAKRATDGKPNCGLCINICPYGRKQEGREKSRHEF
                     VHSQ"
     gene            complement(2106..2798)
                     /locus_tag="VFK23_12215"
     CDS             complement(2106..2798)
                     /locus_tag="VFK23_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811013.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HET7319895.1"
                     /translation="MVTEEKKKELLEKLRNAVIQYDEDTAKEAANEVLSVGMNANDAI
                     FNGLVSGMVEVGRMFEAQEYFVPELLMCADALYAGLDILKPHVEQLDLGVKGSVVIGT
                     VEGDVHDIGKNIVKMMFDVAGFTVYDLGRDVPLDKFVAEQLRTDSDLVCLSAMMTTTM
                     TGMKKVIDDLRTKNPNVKIMIGGAPVSQDVAEKYGADGYAKDATNALKDAINMVASLK
                     KMKDEMEAKKAK"
     gene            complement(2804..>3302)
                     /locus_tag="VFK23_12220"
     CDS             complement(2804..>3302)
                     /locus_tag="VFK23_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HET7319896.1"
                     /translation="KLLEQLTGVIIQGAEEYQKAGVDYITVREMGATSDVLSPRVFKN
                     LILPYLKQIFEKITVPSALHICGKTNDIVPFMAESGTNAISVDQKNDVAESRKKLGPK
                     ALVFGNYDPYNVLVAGTPDLVRQTVKKCLDDGVSAVWPGCDIWPTVPADNFKAMMDEV
                     RNYRR"
BASE COUNT          560 a         1016 c          956 g          770 t
ORIGIN      
        1 cttcgcgtca accttcgcga tgtgcgtgtg ccagtccgcc ttgccgatga ccttgtaccg
       61 gggctccttg gtctcgggat cgatctgcac cgtctcgagg gcgccggggg ccgcccgcat
      121 gccgaacttg atctgggcgc cctcaaaggc cggtcccgtg gcgcaagacg tggagcagac
      181 cttgtcccgg ttgcccatga gcagttcgcc gttcgtgccg atgtcgatga tcagcacctt
      241 ctcgtcctgg ttgtagggtt cctcggcgat caagacgccg acattgtccg cgcccacgaa
      301 tccggcctcg atgggcagca cgtgaatata cgctgcggga tttatccgaa gaccgacgtc
      361 gcgcgccttg atatcgagcg aggtctggac cgccgggata aagggagagc ggccgatgta
      421 ctcggggttc aagcccagga agatgtggtg catggccgtg ttgaacacga tcgtgaggtc
      481 gtcgatgacg tgccccgggt tgggcccgtc cttcctgatc tcggtaacga cgcgttcaat
      541 gatctcgttc agaccctgga tgatggcctt ctgcatggtc tccaggccca cgccgggatt
      601 gctcatggca taggtgatgc gcgacatcac gtcctcgccg taaggcacct gggggttcat
      661 catggactcc gtgttcacca ctttgcccgt gttcaggtcc gtgaggtagc cgacgcaggt
      721 cgtggtgccg atatccacgg caagcccgta gctctcctcg acgaaccccg gctccgcctt
      781 gatgatctct ttgtccatcc agaccgtcat cgtggccttc catccgccct tgcgcagcac
      841 atcctggagg ttcttcagca ccaggtagtc gaaggtgagg cccttgagcc cgtagtcagc
      901 ctcaaggaac atcaggaccc gctcccagtc tccggtggtc atgtcatgga gcgtcggtgc
      961 gacgaggtcc acgttgtatt tctttaccgc ggggtcaagc ttgatcgtca gctccttggc
     1021 cgctttcctg acgacctgtt tgccggcgcg ggagcgttcg ggcacgaaga ccttcacgtc
     1081 gccccggatc tcgcactggc aggcgagcct gatgcccggt ccgtcctggg gtttgatgtg
     1141 cttcatctca tcgggagacg gcacagcggg gtagatatgg gccatgccgg aatcgatatt
     1201 gtccttctcg acgtaccctt cctcgatgcg gaccttgcac ttgccgcaga ccttcttatt
     1261 gccgcacagc gcctcgatgt caacgcccag ggactgggca gcttcgagga tggttttgcc
     1321 ctccgcgacc tcgccgcgcc tgcccgaggg ctggaatatg accttatgat tcttagacat
     1381 tcacttcctc cagtactatt cttaaatgat aaaatgatcc tgatccgagc tctttcggga
     1441 ttattgagaa tgcacaaact cgtgccggga tttttcccgc ccctcttgct ttctcccgta
     1501 cgggcagatg ttgatgcaca gcccgcagtt gggtttgccg tccgtggccc gcttggcctt
     1561 cttgtggctc ctgcagacgt cgagtctcac gagctccaca aaggggaagg agcgcgacca
     1621 ttcagcgcct gtgattgcct ttgacggaca gtaggcagcg cagagcgaac aatcgccgca
     1681 caagctgtgc tctaccggct cgtccggctc gaagggtgcg tcggtcagca cggtaaccag
     1741 cgagagccgc ggaccgtagt cagggctgat gaggagtccg ttcctgccga tccagcccag
     1801 ccccgcggaa gtcgctgcca tcttgtggtt gaagagggag taaagtttcg aaacgaacgt
     1861 cccctttttc cggtccgaat ccggcgggat ggcaagcgag cggtatcccc gcgacttcag
     1921 gaaccggacg gcgcgcttct ggagtttcat gaggatgctc agggtgtctt cattgagcct
     1981 gcgggctacg ccgatcgaca cggcctgctc cagatgggcg atctcgccgt tcatgtaccc
     2041 cttgaggccg gcgaatccca cgacatcggc gccgagaccc tcaagctctt ttcggagctc
     2101 tgcgttcatt ttgctttctt tgcttccatc tcatctttca ttttcttgag cgaggcgacc
     2161 atattgatgg cgtccttcag ggcgttggtc gcgtcctttg catacccgtc ggcgccgtac
     2221 ttctctgcca catcctggga taccggtgcg ccgccgatca tgatcttgac gttcgggttc
     2281 ttggtcctga ggtcgtcgat caccttcttc atgcccgtca tggtcgtcgt catcatggcg
     2341 gaaaggcaga cgaggtcgga gtcggtccga agctgctcgg caacgaactt gtcgagtggc
     2401 acgtctcggc cgaggtcgta gaccgtgaag cccgccacgt cgaacatcat cttcacgatg
     2461 ttcttgccga tgtcgtgcac gtcgccctca acggtcccga tcacgacgga ccccttgacg
     2521 ccgaggtcga gctgctccac atggggcttc aggatgtcaa ggcccgcata gagcgcgtcg
     2581 gcgcacatga ggagctcggg cacgaagtat tcctgggcct cgaacatgcg acccacctcg
     2641 accatgccgg acacgagacc gttgaaaatg gcgtcgttcg cgttcatgcc cacgctcagg
     2701 acttcgttgg cggcctcttt tgccgtgtcc tcgtcgtact gaatcacggc gttccgcagt
     2761 ttttccaaca attctttctt cttctcttcc gttaccatta ggattacctc ctgtaatttc
     2821 tgacttcatc catcatcgct ttgaaattat cggccggcac cgtgggccag atgtcgcatc
     2881 cgggccagac cgcgctcacg ccgtcgtcca gacacttctt gaccgtctgg cgcacgaggt
     2941 ccggcgttcc cgccaccagc acgttgtagg ggtcgtagtt cccgaagacg agcgccttgg
     3001 ggccgagctt cttgcgcgat tcggcgacgt cgttcttctg gtccacgctg atcgcgttcg
     3061 tcccgctctc ggccatgaag ggaacgatgt cattcgtctt gccgcagatg tgaagtgcgc
     3121 ttgggaccgt gattttttcg aagatctgct tgagatacgg aaggataagg ttcttgaaca
     3181 cgcggggaga gagcacgtcg gaggtcgccc ccatttccct gacggtgata tagtcaaccc
     3241 cggccttctg gtactcctcc gccccctgga tgatcacgcc cgtaagctgc tcgagcagtt
     3301 tc
//
LOCUS       DASSLD010000676         4093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_832057,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000676 DASSLD010000000
VERSION     DASSLD010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832057"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VFK23_12225"
     CDS             <1..850
                     /locus_tag="VFK23_12225"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HET7319897.1"
                     /translation="PEANTALKSYLFDLVKSRVTIGIEPGVFSSAFNGMVMYVDKMDE
                     KNNMEGVFISDERTDAEHYTIIARRGTLMADPRTLNVTLTLQEGSILTAPRKDQSYPL
                     MGFSQAKLFIDVKSSLAGRDSPGRGLEDMSLDELRREIGKTRSEGKPTYTQETELHKR
                     LSIPFACLIFGLIGAPLGIRRSRSGKSAGVAIALFVFLVYYIVVGGATNLAETGKFPP
                     PLAFWIPNSVMAVASLLFVFKKGHELNLGIGSALAKLYYGSRERNRKKAEAGLRNNDK
                     GTRQLP"
     gene            847..1932
                     /gene="lptG"
                     /locus_tag="VFK23_12230"
     CDS             847..1932
                     /gene="lptG"
                     /locus_tag="VFK23_12230"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HET7319898.1"
                     /translation="MTVLSRYIFKQFITLVAGVLLGILVVYLCVEFLQKADKLIKFNA
                     TPSQVARFFLFSIPGWISISLPMATLIGALLSLGGLSRHNEIIAMRASGVSLGRIIAP
                     MIAGGLVIAAVSFLNNEFIMPVYSSRALYIRSVEIEKKQQRVMFQRQKLWLRGPDNSI
                     ANIEMISPERNEMLGLNIYKLNSDFSVRERITAGSLLWQDGAWRLQHSRTYAQNGDTV
                     TIYSSDGQIFNIVDAPEDLRMVVKDASEMNFPEMWDYVRRIKTSGYKAGMYEVQLHQK
                     IAYPLSSLLMVLISIPFSVHKVRSGGAAKGFAFAVMIAFFYWTLMSVGESLGRSGAVS
                     AVVAAWLANACFGVTGAVVLIRMQRTI"
     gene            1974..2633
                     /locus_tag="VFK23_12235"
     CDS             1974..2633
                     /locus_tag="VFK23_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319899.1"
                     /translation="MKILLGLALVPACLGFTRQLGATVFSVAYKPDAPYVFLAGGLAY
                     LTVHLLFRKPIFTYVIGHELTHALFAMLFGGSVKSLSAGERGGRVTVTKSNFMITLAP
                     YFFPLYTVVAMACYGAARAWGAGATATNTIIFVSGAAFSFHLVLTFMFLLADQTDIIE
                     QGAAFSYPLIYLFNVVFAAFLVNVYLAEDMDYVAFIAGGLADSANMVAGLARKAYAII
                     R"
     gene            2760..3374
                     /gene="coaE"
                     /locus_tag="VFK23_12240"
     CDS             2760..3374
                     /gene="coaE"
                     /locus_tag="VFK23_12240"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET7319900.1"
                     /translation="MLLVGLTGGIASGKSLVAGVFKDLGAHLVDADRIVHELLEPGSE
                     AAREVLDCFGEEILLPDGRIDRRKLGEIVFNDAAKRARLNQCLHPKVFESYQAQVRQV
                     GNRSPQAIIVLDAALLIETGYHRGMDRVVVVYAEPEQQLERLISRNGFTREEALARVQ
                     SQMPLAGKRDFADYVIDNTGTRERTQQQAREVYSRLKQEAEKRS"
     gene            3406..4086
                     /locus_tag="VFK23_12245"
     CDS             3406..4086
                     /locus_tag="VFK23_12245"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7319901.1"
                     /translation="MTALKNIKNIDLFKNLSDSELKELTSYLVATTYKKKEEIFSEGE
                     QPEWFYIIAKGKVKITKLSHDGKEIILELISPTDIFGGVAVLRNFPYPANAVAMEETE
                     VIKISRKNLMRLVDRFPNLMYCIALQLGDRMKSSYDSLKNIALERVEARIAALLLKLA
                     GKVGIETTEGLLIDMRLTKQDVADMVGTTVETSIRTFSKFKKQGFLSDKDGKIVIRDR
                     DGLEELAS"
BASE COUNT          876 a         1225 c         1168 g          824 t
ORIGIN      
        1 cccggaggcc aacaccgcgc tcaagtccta tcttttcgac ctggtcaagt cgcgcgtcac
       61 gatcggcatc gaacccggcg tcttcagcag cgccttcaac ggcatggtga tgtacgtgga
      121 caaaatggac gagaagaaca atatggaagg cgttttcatt tccgatgaac gcaccgacgc
      181 ggagcactac acgatcatcg cccgtcgcgg cacactcatg gctgaccccc ggacgctgaa
      241 cgtcactctc accctgcagg aggggtccat tctcaccgct ccccgaaaag accagtccta
      301 cccgctcatg gggttttccc aggccaaact cttcatcgat gtcaagagct cccttgccgg
      361 aagagactcc cccggcaggg gcctggagga catgagcctc gatgaactgc gacgggaaat
      421 cgggaaaacg cgcagcgagg ggaagccgac ctatacccag gaaacggagc tgcacaaaag
      481 gctctccatc cccttcgcct gcctcatctt cggactcatc ggggcaccgc tcggcatcag
      541 aaggagccgt tcgggtaaat cagccggcgt cgccatcgcg ctttttgtgt ttcttgttta
      601 ctacatcgtc gtgggagggg cgacgaacct cgccgaaacc ggcaagtttc ctccccccct
      661 ggcgttctgg attcccaaca gcgtcatggc cgtcgcttcg ctgctttttg tgtttaaaaa
      721 agggcatgaa ttgaatctgg gaatcggctc cgccctcgcc aagctgtact atgggagcag
      781 ggagcggaac cggaagaagg ccgaggccgg gctccgaaac aacgataagg gaacgcgcca
      841 actgccatga ccgtcctcag cagatatatt ttcaagcaat tcatcaccct cgtcgcgggc
      901 gttctcctgg gcatcctcgt cgtttacctg tgcgtggagt ttctccagaa ggcggacaaa
      961 ctcatcaagt tcaatgccac cccttcgcag gtggcccggt tctttctctt cagcatcccc
     1021 ggctggatct cgatatcgct gcccatggcg accctgatcg gggcgctcct gtctctcggc
     1081 ggactttcgc gccataacga gatcatcgcc atgcgcgcca gcggcgtgag cctgggcagg
     1141 atcatcgctc ccatgattgc cggcggtctc gtcattgccg cagtcagctt cctcaacaac
     1201 gagttcatca tgcccgtgta cagttcgcgc gccctctaca tccgcagcgt cgagatagaa
     1261 aaaaaacagc agcgggtcat gttccaaagg cagaagctgt ggctccgggg cccggacaac
     1321 agcattgcca acatcgagat gatctccccg gagcgcaatg aaatgctcgg cctgaacatc
     1381 tacaagctga attcggattt ttccgtccgg gaacggatca cggccggcag cctcctgtgg
     1441 caggacggcg catggaggct gcagcacagc cgcacctatg cgcaaaacgg cgatacggtt
     1501 accatctact cctccgatgg gcaaattttc aacatcgtcg acgccccgga agacctgcga
     1561 atggtcgtca aggacgcttc ggaaatgaac tttcccgaga tgtgggatta tgtcaggagg
     1621 atcaagacga gcggctacaa ggccggcatg tatgaagtcc aattgcacca gaagatagca
     1681 taccccctgt ccagcctgct catggtattg atatccattc ccttttcggt ccacaaggtg
     1741 cgaagcggag gagcggccaa ggggttcgcc tttgccgtca tgatcgcctt tttctactgg
     1801 acgctcatga gcgtcggcga gtcgctcgga cgctccggcg cggtttccgc cgttgtcgcg
     1861 gcatggcttg cgaacgcctg tttcggcgtc acgggagccg tcgtgctcat ccgcatgcag
     1921 aggacgatat gaggaaggcc gggtaatctc ttctgaccat gacgaagcgc ttcgtgaaaa
     1981 tcctgctggg gctcgccctt gtccccgcct gcctgggttt tacccggcag ctgggcgcga
     2041 cggtattttc cgttgcctac aagccggatg cgccctatgt cttcctggcc ggcggcctgg
     2101 cgtatctgac cgttcacctg ctcttcagga agccgatctt cacctacgtc atcgggcatg
     2161 agctcaccca tgccctcttt gccatgctct tcggcggatc agtaaagtcc ctctcggccg
     2221 gagagcgggg aggccgcgtg accgtgacca agtcgaattt catgataacc ctggccccct
     2281 attttttccc tctctatacg gtcgtcgcca tggcatgcta cggcgccgcc agggcctggg
     2341 gcgccggcgc gacagcgacg aacacgatca ttttcgtgag cggcgccgcc ttttcgttcc
     2401 atctggtgct gaccttcatg ttcctgttgg ccgatcagac ggacatcatc gaacagggcg
     2461 ccgccttttc ctatccgctc atctacctgt tcaatgtcgt gttcgcggcg tttctcgtca
     2521 atgtgtattt agcggaagac atggattacg tggcgttcat tgccggcggc cttgcggaca
     2581 gcgcaaacat ggtcgccggc cttgccagga aggcatatgc aattatacgg taacattctt
     2641 ctttcccgga agacgctatc cggtatccgg ttttctgcgg atgccgtatg cccgatagaa
     2701 cccttcgggg atgacaatca ttccggtagc ggccaaaacc ggtgtctaaa aggccaatca
     2761 tgctcctggt aggactgacg ggcgggatag ccagcgggaa gagcctcgtg gcgggtgtct
     2821 tcaaggatct cggcgctcac ctcgtcgacg ccgaccggat agtccacgag cttctggaac
     2881 cgggcagtga agcggcgcgg gaggtgctcg actgtttcgg cgaagagatc ctgctgccgg
     2941 acggccgtat tgaccggaga aagctcgggg agatcgtctt caacgacgcg gcgaagaggg
     3001 cccggctcaa ccagtgcctt cacccgaagg tctttgagtc ctaccaggcg caggtcagac
     3061 aggtaggcaa tcgttctccc caggcgatca tcgtcctgga cgccgcgctc ctcatcgaga
     3121 caggctacca ccgcgggatg gaccgcgtcg tcgttgtcta cgcggagccg gagcagcagc
     3181 tcgagaggct gatctcccgg aacggcttca cgagggaaga ggcccttgca cgggtacaga
     3241 gccagatgcc gctcgcggga aagcgggact ttgccgatta cgtcatcgac aacacgggga
     3301 cccgcgaacg gacccagcaa caggccaggg aagtttattc aagactgaaa caggaagcgg
     3361 agaagcgttc ctgattcatc agactaccgg accaaggaga atggaatgac agcgctgaaa
     3421 aatataaaga acatcgacct gttcaagaac ctctccgata gtgagctgaa ggagctgacg
     3481 tcctacctag tcgcgacgac gtataagaaa aaagaggaaa tattctcgga aggcgaacag
     3541 cctgagtggt tttacatcat cgcgaagggc aaggtgaaga tcacgaagct ctctcacgat
     3601 gggaaggaga tcatcctgga gttgatatct ccgaccgaca ttttcggcgg agtcgcggtg
     3661 ctgcggaact tcccctaccc ggccaacgcc gtcgcaatgg aagaaaccga ggtcatcaag
     3721 atatcccgga aaaacctcat gcgcctcgtg gaccgcttcc cgaacctcat gtactgcatc
     3781 gccctccagc tcggggaccg gatgaaaagc tcctacgact cgctcaagaa catcgccctg
     3841 gagcgagtcg aggcccgcat cgccgcgctg cttctgaaac tcgccggcaa ggtcggcatc
     3901 gagaccacgg agggtctgct catcgatatg aggctcacca agcaggacgt ggccgacatg
     3961 gtcggcacga ccgtcgagac ctcaatccgc accttcagca aattcaagaa gcaggggttc
     4021 ctgtcggaca aggacggcaa gatcgtcatc agggatcggg acgggctgga ggagcttgcg
     4081 tcttaatatt gca
//
LOCUS       DASSLD010000677         3762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_180132,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000677 DASSLD010000000
VERSION     DASSLD010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180132"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..847
                     /locus_tag="VFK23_12250"
     CDS             296..847
                     /locus_tag="VFK23_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7319902.1"
                     /translation="MEKVLIAVDDTQGSRATITALRNMIRKPRTVILVTVQRLEGDSL
                     VIDMLGEAEMKTLRESLKDTEHQEALDRRARNILDTYAWELWQEGITVKKMVRDGPPA
                     EEILKVALEERVDLIIIGYSCKNFFTRLIKGSVSREIRKNGLVPFLIAKNGTCQEQST
                     GRGARGWVKGRAWQPAQTKRLFY"
     gene            complement(1038..1607)
                     /locus_tag="VFK23_12255"
     CDS             complement(1038..1607)
                     /locus_tag="VFK23_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7319903.1"
                     /translation="MSTSKLLSALVLMAAACGGPHVPTTEYLPLHTRENQSFSGPNLG
                     QEVKPEETAAWDISIPPDGSGLPPGSGTAPEGEAVYNAKCASCHGVEGIKGPADTLVG
                     GIGTLATKKPLKTVGSYWPYATTLFDYIRRAMPFNAPQSLSNDEVYAVSAYLLCLNGI
                     IDPEMRMDARTLPQVKMPNRDGFVSYWPQ"
     gene            complement(1594..2901)
                     /gene="soxC"
                     /locus_tag="VFK23_12260"
     CDS             complement(1594..2901)
                     /gene="soxC"
                     /locus_tag="VFK23_12260"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HET7319904.1"
                     /translation="MKRKVSAELPAPAADRGLLDRRAFLTGAVKVAGLAAIGNLSGDI
                     LATAQAAEPPALETPTWMKIPGQPVRGYGQPAKYEEGVRRIFYQRYKDIAPGAGMSMT
                     PLHQLQGTITPNGLHYERHHNGVPDIDPTRHELLIHGLVKRPLKFSVEALLRYPTISR
                     IYFLECSGNSLFNARPEPPQMTCGAIHGLVSCCEWTGISLALLLDEAGLDPKGKWIVA
                     EGADAAAMNRSIPIEKALDDAMIALYQNGERLRPEQGYPLRLLLPGWEGNMNVKWLRR
                     LTVTEKPVYSREETSKYTDLMPDGRARQFTFPMGVKSVITHPSLGMTMKGKGFYEVSG
                     LAWSGSGRITQVEISADGGKSWARAELQAPILPLALTRFRLPWNWDGGPVLLQSRATD
                     EKGNIQPSREKWAAQYSPGNEYHYNAIQTWSINPEGEIRNVYL"
     gene            complement(3161..3562)
                     /locus_tag="VFK23_12265"
     CDS             complement(3161..3562)
                     /locus_tag="VFK23_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319905.1"
                     /translation="MKVKSIKDAAKKKATKPEKVVDIKKAAKKLDLSSQNMKTQNVKD
                     ATKKKAIKPEKAEDICEFAVKTEEDTKKMMLQDIIDIATRMGIDAGDLNRTELVRAIQ
                     RAEGYSDCFMTDQVRTCGQLNCLWYQECVLC"
BASE COUNT          888 a          953 c          992 g          929 t
ORIGIN      
        1 ttataaccag aagataatta tctgcagcaa ccatatccta gattaaaaca cctacatatc
       61 cacattgagt aaggcgggat agcacggagc agggatattc gatccagaga gggcgggaca
      121 tgacgcaaga atatcgggca aaaagaacta agttaaacat aaaagatccg acccgagatt
      181 ttatttcttt cggcctgtta taataaggat agggatggac ttcttttgat cggggtacct
      241 caactgcaga aagaagaaaa aaaggaaagg ttaggaacga aaaggaggat ggcctatgga
      301 aaaggtcctc attgcggtag atgacacgca gggttcgcga gccacaataa ccgctttacg
      361 gaacatgatc aggaagcccc ggaccgttat cctggtgacc gtccagcggc tcgaaggcga
      421 ttcgcttgtc atcgacatgc taggagaggc cgagatgaag acattgcgcg agtcgttgaa
      481 ggataccgag caccaggaag cgctggaccg gagggcccgg aatattctcg atacttatgc
      541 atgggagctc tggcaggagg gcataaccgt gaagaaaatg gtgagggacg gcccaccggc
      601 cgaggagatc ctgaaggtcg ctctggagga gcgtgtggac ctcatcatca tcgggtacag
      661 ctgcaagaat ttctttacgc ggctcatcaa gggatcggtg agcagggaga tcaggaagaa
      721 tggtctggtc ccgttcctga tagcaaagaa cgggacatgc caggagcagt caacggggcg
      781 cggcgcgcgt ggttgggtaa aaggcagggc atggcaacct gcgcagacca agcggctatt
      841 ctattgatcc tccggagtgt acgcttagcc gaaaatgaac agcgtgatgg gatcgtgaca
      901 cccagtacca tgccaatggg ccgataccga gcaatgtcgt attggtaaat gttatcaatt
      961 tcgagataag atgttgattg ttgtcaaaga taagaactcc attatctcag gatgactcat
     1021 ttctccgccc ctgtttctca ctgtggccaa taactgacaa agccgtctcg gttcggcatt
     1081 ttaacctgag ggagcgttct tgcgtccatc cgcatctccg ggtctatgat gccgttgagg
     1141 caaaggagat aagcgctcac ggcgtaaact tcgtcgtttg agagggactg aggagcattg
     1201 aaaggcatgg ccctgcgaat atagtcgaac agcgttgttg cgtagggcca atagctcccg
     1261 acggttttta aaggcttctt cgtggccagc gtgcctatcc ctcccacgag agtatcggcg
     1321 ggtcctttta tcccctccac cccatggcaa cttgcgcatt tcgcgttata aactgcttct
     1381 ccttcaggag ccgtaccgct gccggggggc agtccgcttc catcgggcgg gatactgata
     1441 tcccaggcgg cagtttcttc gggcttgact tcctgaccga gattgggacc ggagaaggac
     1501 tggttttccc gggtgtgcaa cgggagatac tcggtggttg gaacatgagg tccgccgcag
     1561 gcggcagcca tcagaaccaa ggcgctcaag agtttagagg tagacatttc ggatttctcc
     1621 ttccgggttt atactccagg tttggatcgc gttgtaatga tattcgttgc ccggcgagta
     1681 ctgtgcggcc catttttcac gggagggctg gatgttccct ttctcatcag tggcccggct
     1741 ttgtaacaac acggggcctc cgtcccaatt ccagggaagt cgaaaccggg tgagcgccag
     1801 cgggagaatt ggagcttgca gctccgctct tgcccagctc ttgccgccat ctgcggatat
     1861 ctcgacctgg gtgatgcggc cggagccgga ccaggcgaga ccggatacct cgtaaaaccc
     1921 cttccccttc atggtcatgc ccaaggaagg atgagtaatg accgacttga ctcccatggg
     1981 aaaggtgaat tggcgggctc ttccatcagg catcaaatcg gtatacttcg aggtttcttc
     2041 ccggctgtat acaggttttt ccgtgaccgt aagccggcga agccatttaa cattcatatt
     2101 cccctcccag cccggcagca ggagcctcaa gggatagcct tgttcaggac ggagacgctc
     2161 gccgttctgg taaagtgcga tcatggcatc gtcgagggct ttctcgatgg gaatacttcg
     2221 gttcatagca gcagcgtccg caccttcggc cacgatccat ttgcccttgg ggtcaagtcc
     2281 agcttcgtcc aataatagag ccagggagat acctgtccat tcacaacagg aaaccagccc
     2341 gtggatggct ccacaggtca tttggggcgg ttccggtctg gcgttgaaaa ggctgttgcc
     2401 tgaacattcc aaaaagtaga tccggcttat agtcggataa cgtagcaaag cctcgacaga
     2461 gaatttcagc ggtctcttga cgaggccgtg gatgagcaac tcatgccgtg taggatcgat
     2521 gtccggtacg ccgttgtgat gtcgttcata gtgcaacccg ttcggagtaa tcgtaccctg
     2581 gagctgatgg agaggagtca tggacatccc ggcgcccgga gcaatatcct tgtatctctg
     2641 gtagaagatc cgcctgaccc cctcttcgta tttggcaggc tgtccgtaac cccggaccgg
     2701 ctgtccgggt atcttcatcc aggtcggggt ttccagcgcc ggaggctccg ctgcctgcgc
     2761 cgtcgccaaa atatcacccg aaagattgcc tattgccgcg agtccggcca ccttgaccgc
     2821 gcctgtcaga aaagccctgc ggtcgaggag gccccggtct gccgcgggtg caggtagttc
     2881 cgctgatact tttctcttca ctgttctcca ccgagatgtc atcgcccacc tcctatctct
     2941 ttgactatta cgaccgaact ggaagggcaa aggttgctaa actttgcgaa taggcagttt
     3001 tcacgcagcc aggtcctttt ttctgacccc ggcgcaccag acgcgaaaca gtgcttaaat
     3061 ccaatgcgta tttgaaggta aaactcgtac cataaaaaaa ccctgagtaa tatattctac
     3121 tcagggtttc agggaagaat tctatagaaa caacctcact ttagcatagt acacattctt
     3181 gataccacag gcaattcagc tgaccgcagg tccgaacttg atctgtcata aagcaatcac
     3241 tatacccttc ggccctctgg attgctctaa caagctcggt tctattcaaa tcccctgcat
     3301 caatccccat ccttgtcgca atgtctatga tgtcctgaag catcattttc ttagtgtcct
     3361 cttccgtctt tactgcaaac tcgcagatgt cttcagcttt ctcaggtttt attgccttct
     3421 ttttcgtggc atctttgaca ttctgagtct tcatgttctg ggaggacaga tccaactttt
     3481 tggcggcttt ttttatgtcc acaacttttt caggctttgt tgctttcttt tttgcagcgt
     3541 ctttgatgct cttaaccttc atttattgcc ccctcatttt cgaaattaca gacaaaaatt
     3601 agtatagcac aacagagata gcttcgcggg aagtagctgt ggttttttat gccttaacgg
     3661 tgaggattcc tcggctatgc ctacatcaac acgaaatgca gtcgtgagaa gaatggaata
     3721 atacggccac taattcaatg ttaatgaagt tgttaattta tg
//
LOCUS       DASSLD010000678         4201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1249612, whole genome shotgun sequence.
ACCESSION   DASSLD010000678 DASSLD010000000
VERSION     DASSLD010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249612"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..499)
                     /locus_tag="VFK23_12270"
     CDS             complement(116..499)
                     /locus_tag="VFK23_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319906.1"
                     /translation="MSTASSRPAGWRILGFGKHPEIAATIQEKLRSLWFQATNFVLTD
                     DEAGDARLVAELKRAEYDGVAIGGYIYGQDAVNFPATEETTVWFNRVLNIIHANAPGS
                     KIILVRGPEDVVPAIERVLGKTPPS"
     gene            848..1378
                     /locus_tag="VFK23_12275"
     CDS             848..1378
                     /locus_tag="VFK23_12275"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7319907.1"
                     /translation="MVTTGERAAARLIRADLDATRATVRSLLDSFSQEDWRKQSLNPG
                     WANGEVMAHMVFGFVVVIALLRLTRLWGRLPKDSSRAFARMLNRFTGPFNSVNAFGAR
                     IQGGLFAPDRIGAVYDWAAGLLLKKAATIGDAEWERGMYYPTRWDSNFSEFMTLEKLF
                     RYPVTHFNFHLRQIAR"
     gene            1569..1775
                     /locus_tag="VFK23_12280"
     CDS             1569..1775
                     /locus_tag="VFK23_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319908.1"
                     /translation="MSQKTYFLVCSAVFFVVAAAHLTRLIVGWEITIAGWIAPQWISI
                     PGLIIPGVLSAWGFVLASRPRQTE"
     gene            1939..2610
                     /locus_tag="VFK23_12285"
     CDS             1939..2610
                     /locus_tag="VFK23_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319909.1"
                     /translation="MRARRYFTGITILGIIPITLLSFASPVFAGSGWTIEFLVGIPFT
                     FPSSLAIRQAGEPDLRHQPRFQSRPFDRPIYYTVRIGKWDGPDGWELEMNHDKLYLSN
                     PTKEIEQFAISHGFNRITLNRSMERGDVIWRVGAGVVITHPENTVRGRRVPEDEGILG
                     TGYHLSGPTAMGAVGARISSREGPFLPLEGMGSASWVRVPVADGEADLAVAGLHGLVG
                     VGYGW"
     gene            2950..3720
                     /locus_tag="VFK23_12290"
     CDS             2950..3720
                     /locus_tag="VFK23_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HET7319910.1"
                     /translation="MIRKIFFSGLVLAMFMIPTLGFAQPSPDAEEQSLLSAFMSYTDL
                     YMRSVQQSLEILASTTEVTSGKWENMKGVIEVYQKSDGKLIVWYVLPDGRYYTVDKGL
                     MDVKLSDRSYFADLMAGKKIIGSLVVSKSTGQRSAVIALPIKEGGKVVGGIGASIFLD
                     KLSEEIGSALNLRPDTSFFALAPNGQTTLHRKTDRHFLDPREQGSETLKKTANEMLSN
                     NAGESTYIFDNVNKKAIYRTSPLTQWKFAIVFSAIPLK"
     gene            3782..>4201
                     /locus_tag="VFK23_12295"
     CDS             3782..>4201
                     /locus_tag="VFK23_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET7319911.1"
                     /translation="MKKLFLIALALSMLHEVAAADSSCPSLVITGHPSYPPVAWGSDS
                     EIVGAAPEMVANIARTLGVKKVTSRNFGSWEKAQSAAKNGEADIIFGIYKNDERMKWL
                     DYVEPAFMMDPVSVVVRRGEGFAYAKWDDLKGRKGVTN"
BASE COUNT          930 a         1171 c         1185 g          915 t
ORIGIN      
        1 ccgtgcggat tcccctatgg gactgataag ggcctccaga ctactcaaga ggcctttctg
       61 ttctcatcat ggtgccgaag gggagtcgaa ccccactggt tttcctgcct ccccctcaag
      121 atggcggggt cttaccgagc acccgttcaa tcgccggcac aacgtcctcc ggaccgcgca
      181 caaggatgat cttcgatccc ggcgcattcg cgtggatgat gttgagaacg cgattaaacc
      241 agaccgtggt ctcttcggtc gcagggaaat tcacggcatc ctgtccgtag atgtagccgc
      301 cgatggccac gccatcgtac tcagctcgct tcagctccgc gaccaaccgc gcgtccccgg
      361 cttcgtcgtc cgtgagcacg aagttggtcg cctggaacca gagcgagcgg agtttctcct
      421 gtatggtggc cgcgatctcc ggatgtttgc cgaacccgag gatgcgccaa cccgcagggc
      481 gtgaggaggc ggtcgacata ttaaaattga accacggcat gtcgtccctt gcaagacgtc
      541 aaccccaaga acagcctccg catgaattcc ctttttcacc ccgaaaaagc gagttgtttg
      601 cacggggttt gcagtccgtg cggcatctca tcatcaagac ttcgcccccc tgaaatcggc
      661 ctgagatcct taacacacct cggaccgatt tgagggtagt catgtcatgt gtcggtcgtg
      721 atctgcaccc ttccgcttgt ctcgtagcat tctatccggc tggcgatgtt caccgtgtcg
      781 cctcacaggt cgtaggcgca tcttatgcag agaaggacat catggactag ggggagcccg
      841 cagtaccatg gtcacaacgg gtgaaagggc tgcagcacgg ctcattcggg ccgatctcga
      901 cgcgacccgg gcgacggtcc gttccttact cgactcgttc tcgcaggaag attggcgtaa
      961 acaaagtctt aacccgggct gggccaacgg cgaagtcatg gcccacatgg tattcggatt
     1021 cgtcgtcgtt atcgcactac tgcggttgac ccgcctgtgg gggaggctgc ccaaagactc
     1081 ctcgcgagca ttcgcccgga tgctcaatcg gtttaccggg cccttcaaca gcgtcaacgc
     1141 cttcggggcc cggatacagg gagggctgtt tgcccccgac cggatcggag ccgtgtatga
     1201 ctgggccgcc ggattgctgt tgaaaaaggc cgcgacgatc ggtgatgcgg aatgggaacg
     1261 gggtatgtat tatcccacca ggtgggactc caatttcagc gaattcatga cgctcgagaa
     1321 actgttccgt tacccggtca cgcacttcaa ctttcatctc cggcaaattg cacgttgaac
     1381 tcggaccccc acgggtttcc ccaccacctc ctttttcacc ccgaaatagg aggttgtttg
     1441 catggggttt gcagtccgtg cgatttccct tctaaatccc gattaaatgc ccgaaaggtt
     1501 attacactaa gatcaggatg gcccaagcat tggaggcggg gcagaatcct tcggactgga
     1561 ggcccctcat gagtcagaag acgtactttc tggtctgcag cgcggtgttc ttcgtggtcg
     1621 ctgcggcaca tctgacacgc ttgatcgtcg gatgggagat tacaatcgcc gggtggatcg
     1681 ctccccaatg gatctcgatt cccggtctca tcattccagg tgttctgtcg gcttgggggt
     1741 tcgtactggc atcgcgaccc agacagacag aatgagattg tcagtggcag gcggagagcc
     1801 acacaacgcc gctcccccta acaacacctt tgtttgcacg ggataacgtt ggaagaacat
     1861 gccggacgat aattcgtagc catacagtta gaattgggta ggaaagacct atatcccgcc
     1921 tgcctgagga ttggccggat gcgcgccaga agatacttca ccgggatcac aatcctgggc
     1981 atcatcccga tcaccttgct ctctttcgcc tctcctgtgt tcgccggatc cggatggacc
     2041 attgagttcc tcgtagggat tcctttcact ttcccttctt ccctggcgat ccgccaagcg
     2101 ggggaacccg atcttaggca ccagccccga tttcagtccc gccctttcga tcgaccgatc
     2161 tattacaccg tcaggatcgg gaaatgggac gggccggacg gctgggaact ggagatgaac
     2221 cacgacaaac tttacctctc gaatcccacc aaggagatcg agcaattcgc gatatcccac
     2281 ggtttcaacc gaatcacgct gaaccggtcg atggagcggg gagacgtgat ctggagggtg
     2341 ggggcaggag tggtgatcac ccacccggaa aacacggtac gtgggcgacg agtccccgag
     2401 gacgagggga tcctggggac ggggtaccat ctttccggcc cgacggccat gggtgccgtc
     2461 ggtgcccgga tcagttcgag ggaaggcccc ttcctccccc tggagggaat ggggtcggcc
     2521 tcctgggtgc gggtcccggt cgcggacggc gaagcagacc tcgcagtggc aggcctccac
     2581 ggcctcgtgg gagtgggata cggttggtga tctgactcca aaatcccttc ggccaattgt
     2641 gatcgcccaa tgcgggactc gccccccacg ggtttcccca ccaccccatc aagatgggca
     2701 ttgtttgcat gaggttggca ttccgtttca ccctaagata ggggtggact gatcacggag
     2761 gcaggtcatc ccgaattgca agaaatatat tgacgagtga aaaccggggg tatgtaatat
     2821 taatgtaaat ggatatagtt cagggaacca gccgaaggaa cgcccgcata attgttttca
     2881 atcgccaacc ccttgcagga agtaggtact ggaaaaaccc tgggtgattt tgactaagga
     2941 ggcaagaaca tgatcaggaa aatctttttc agcggtctgg ttttggcaat gttcatgata
     3001 ccaacccttg gcttcgccca accttccccg gatgcggagg agcaaagcct gctgtctgcc
     3061 tttatgtctt acacggacct gtacatgagg tcggtacagc agagccttga aattctcgcc
     3121 tccacaaccg aagtgacctc cgggaaatgg gagaacatga agggcgttat cgaggtctac
     3181 cagaaatcag atggtaaatt gattgtctgg tatgtcctcc cggatgggag atactatacc
     3241 gtggacaagg gactgatgga tgtgaaactg agtgatcgca gctactttgc tgatctcatg
     3301 gcaggcaaaa aaatcatagg gtctctcgtc gtaagcaaaa gcaccggcca gcgttccgca
     3361 gtgatcgcat taccaatcaa ggaggggggt aaggttgtcg gcggtatcgg cgcttccatc
     3421 tttcttgaca agctctccga agaaatcggt tccgccctca atcttcggcc ggatacatcc
     3481 tttttcgccc ttgccccgaa cggccagaca acccttcacc gaaagacgga caggcatttt
     3541 ctggatccca gggaacaggg aagcgagacg ctaaaaaaga ctgcaaacga gatgctctca
     3601 aataatgcgg gagaatcgac ctacatcttt gacaacgtca ataagaaagc catttaccgg
     3661 acttcccctc ttacgcaatg gaagttcgcc atcgttttca gcgccattcc tctgaaataa
     3721 ccggatggag gtctttatcc aagatgccgc acccctgcct acgatacgga ggagtctgct
     3781 gatgaaaaaa ctattcttga tagcgctcgc gttgtcgatg ttgcacgagg tagccgcggc
     3841 tgactcatcc tgtccatcgc tggtgatcac cggccacccg tcctatccgc cggtagcttg
     3901 gggatcagac agcgagatcg ttggcgcagc gcccgaaatg gtggccaaca ttgcacgaac
     3961 gctcggcgtc aagaaggtga catccaggaa tttcggttcg tgggaaaagg ctcagtcggc
     4021 agcgaagaac ggggaggccg acatcatttt cggcatctac aagaacgacg agcgaatgaa
     4081 gtggctcgac tatgtcgagc ctgcgttcat gatggatccg gtatcggtcg ttgtgcgtcg
     4141 gggcgaggga tttgcctacg cgaaatggga cgatttgaaa ggcaggaagg gtgtcaccaa
     4201 c
//
LOCUS       DASSLD010000679         3707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_180234,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000679 DASSLD010000000
VERSION     DASSLD010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180234"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VFK23_12300"
     CDS             complement(<1..211)
                     /locus_tag="VFK23_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET7319912.1"
                     /translation="MARFDKLTLKATEALQAAQENAARRHHQNIEVEHLLLALLEQTD
                     GVVLPLLKKLGVDPVRVKGDIEEALN"
     gene            complement(319..696)
                     /locus_tag="VFK23_12305"
     CDS             complement(319..696)
                     /locus_tag="VFK23_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7319913.1"
                     /translation="MAREHRLFMISVVSEMLGIHPQTLRLYEREGFIKPKRSGGNTRL
                     YSEEDVEKLEMILRLTRELGVNLAGVEVILSMREKMEQMQKEMEETVRQLREELEREI
                     RRREEMRNALVPVRKIRIIRDER"
     gene            complement(811..1941)
                     /gene="dnaJ"
                     /locus_tag="VFK23_12310"
     CDS             complement(811..1941)
                     /gene="dnaJ"
                     /locus_tag="VFK23_12310"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET7319914.1"
                     /translation="MGPATKKDYYEVLGISRNATEADIKKAFRKLARKYHPDVNPGNK
                     TSEQMFKEINEAYEVLSDAKKKQQYDQFGHAAFEQGFGQGAGPGQGFAGFGRSADFSG
                     GYGFGDIIGNIFGERARHRGPRKGEEVTYSDEIDLEDAILGRTMQVDLRRDVACSTCG
                     GSGSQPGTSPRTCPTCMGSGSVTQGRGFMQMSQTCPTCHGEGTINPNPCRICNGRGVV
                     PQSERINVKIPPGVDNGSKVRVSGMGGPGEKGGTPGDVFIVTKVRSHPYFERKGDNLY
                     SEAPVTVKEAALGEKIEIPTVDGMVALSVPAGVQTGQQLKLRGKGVPHLGGGGVGDHY
                     VTIRVVTPTGLSERGKELLRELDRLHPFNPRRDITFRGFRKR"
     gene            complement(2119..2832)
                     /locus_tag="VFK23_12315"
     CDS             complement(2119..2832)
                     /locus_tag="VFK23_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319915.1"
                     /translation="MKNNMLIQQDMRQAVLSALVANDLDAIERLALRGRIVLSVLVRL
                     AYDKETIVGWRAIAATGRVASVFIRNNGEFLRETVRKLLWSLTDESGGIGWSAPELLG
                     EIVSAGPEEFSDVIPIIAGVFSIEEDVFRPGILYAFKRIAEGRPDMISPYQDIILRGL
                     SESSPEARIHALDLIRTLGERIDPAKLKAIASKVRNMVQDRAEAWLYKKDGFVNIEVG
                     SVAQEVNKYFKDKFGLQYN"
     gene            complement(2940..3518)
                     /locus_tag="VFK23_12320"
     CDS             complement(2940..3518)
                     /locus_tag="VFK23_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319916.1"
                     /translation="MESYRYQQLAYLIVPLTAGFEFFVTARTDRKTSGKESTGAVIMD
                     VLGYVFVGIIPALFLFTIYTLESHRLPLLAQLLHRLDRYGVMFFFLGSWWQVFLITSL
                     RARRASAGNEKLLAKVWLPYLAFGAFISALILWVAPWNLMWVSIFWFVGTFALFAVIG
                     VSSSKISKIFLALAILILICENIFFIILDAIV"
BASE COUNT          836 a         1068 c          891 g          912 t
ORIGIN      
        1 tgtttaaagc ttcctctata tcccccttga cgcgcacggg atcgactccg agttttttca
       61 gcaggggcag gacgacgccg tccgtctgct ccaaaagcgc gagcagaagg tgttccacct
      121 cgatattctg atggtggcgc ctggcggcat tttcctgggc agcctgcagg gcctctgtcg
      181 ccttgagcgt tagtttgtca aaacgtgcca ttgtttcctc ctctttaaaa gccgatggac
      241 gagagatgca aaagcttttt cgtccgtagt cctagcgagc gcagcgagcg gtcgtccttc
      301 gtccctcatg tttctacctc atctctcgtc acgtattatt cgtattttcc tcaccggaac
      361 cagggcgttc ctcatctcct ccctgcgcct gatctcccgt tcgagctcct cgcgaagctg
      421 acggaccgtt tcttccatct ccttctgcat ctgttccatc ttctcgcgca tcgagaggat
      481 cacctcgacg cctgcgaggt tgacgccgag ttcccgcgtc agcctcagga tcatttcgag
      541 tttctcaacg tcttcttcgg aatagaggcg cgtgttcccc ccgctccgct tcggcttgat
      601 gaagccctcg cgctcataga gtctcagcgt ctgcggatga atgcccagca tctccgaaac
      661 cacgctgatc atgaacagtc tgtgttcgcg cgccataaga acttcccatt aatgcggaat
      721 gcggaattcg aaatacggaa ttgaaaggta aaagcgcccc ggcgtatccg tttttgtgag
      781 tgctgttttt cttctatgcc ccattccgaa ttatctcttt ctgaaccctc ggaacgtgat
      841 gtccctcctc gggttgaagg gatgcagccg atcaagttct cgtagcagtt ccttccctcg
      901 ttcggaaagc cctgtcggcg tgacaaccct gatcgtcaca tagtggtctc ccacgcctcc
      961 cccgccgaga tggggcactc ctttgccccg gagtttgagt tgctggcccg tctgaactcc
     1021 cgcaggcacc gacagcgcga ccatgccgtc gaccgtcggg atctcgattt tttcccccag
     1081 cgcggcctcc ttgacggtaa cgggtgcctc cgaatacagg ttatcgcctt ttcgttcaaa
     1141 atagggatgg gacctcacct tggtaacgat gaagacgtcg cccggagtac cgcccttctc
     1201 gcccggcccc cccattcccg aaacgcggac ctttgaaccg ttatcgacgc caggcggtat
     1261 cttgacgttg atgcgctccg attgcggcac gacaccgcgg ccattgcaga ttcggcaagg
     1321 attgggattg attgttccct ccccgtgaca ggtgggacag gtctgggaca tttgcatgaa
     1381 gccccttccc tgagtcacgc tgcccgatcc catgcaggtg ggacaagtcc tcggggacgt
     1441 gcccggttgc gagcctgatc ctccgcaggt cgagcaggcc acgtctcgcc gcagatctac
     1501 ctgcattgtc cgcccgagga tggcgtcctc cagatcgatt tcgtcggaat acgttacctc
     1561 ctcgcccttt ctcggccccc ggtgccttgc cctctctccg aaaatattgc cgatgatgtc
     1621 cccgaatccg taccccccgg aaaaatccgc gctccgtcca aaacctgcaa atccctgccc
     1681 cggccccgct ccctgaccga agccctgttc aaacgctgca tgtccaaact ggtcatactg
     1741 ctgttttttc ttcgcatcag agagaacttc ataagcctca ttgatttcct tgaacatctg
     1801 ttccgaggtc ttgttgccgg gattgacgtc aggatggtat tttcttgcca gtttcctgaa
     1861 cgcctttttt atatcggcct cggtcgcatt tctactgatg ccgagaactt cataataatc
     1921 cttttttgtt gcaggcccca taatatacct cagaaatatt ataacgttaa tattataaaa
     1981 tatgagtgtg ttgctgtcaa gttttaagac aaaaaaatat aattttaata tttatttaaa
     2041 aatttgagat aaatcacatg ctatttccca ctattcagag aaatatggct aattatgata
     2101 taacaattac cacttttgtt agttatattg aagtccaaat ttgtctttga aatacttatt
     2161 cacttcctgt gcaactgatc cgacttctat attaacaaat ccatcttttt tgtataacca
     2221 ggcctcagct cggtcttgca ccatatttct aaccttgctt gcaattgctt tcaactttgc
     2281 gggatcaatc cgctctccaa gggtccggat cagatcgagc gcatggatcc tggcctctgg
     2341 ggagctttcg gacagcccgc gaaggatgat atcctgatac gggcttatca tatccggacg
     2401 tccctccgca attcttttga acgcatagag aatgccgggc ctgaaaacat cctcttcgat
     2461 agaaaagacc cctgctataa tcggaatgac gtcggaaaac tcttccggac ctgcgctcac
     2521 aatctctccg aggagttcgg gggcggacca ccctatgccg cccgattcgt ccgtcaggga
     2581 ccagagcagt ttccggaccg tctcccgcag aaactcaccg ttgttcctga tgaacaccga
     2641 cgcaacccgt ccggtcgcgg cgattgcccg ccagccgaca atggtttcct tgtcataggc
     2701 gagacgcacg agcacgctca atactatcct gccgcgaagc gccaacctct cgatcgcatc
     2761 gagatcgttc gccacaagag cggacaggac ggcctgtctc atgtcctgct gaatcagcat
     2821 attgtttttc acggcacgga ctccggtcgc agaaaaatat ccaggtcctc cccccgctca
     2881 tcatggcgga gcaacggatc tagcgaatta tacaccaaat cggatttcgg gggcgattgt
     2941 tatacgattg cgtcgaggat gatgaaaaaa atattttcgc agatcaggat caggattgcg
     3001 agggcgagga aaatcttcga aatcttggag gaagaaactc cgatgacggc gaagagggca
     3061 aaggttccga cgaaccagaa gatggacacc cacatgaggt tccagggggc cacccagaga
     3121 atcaaggcgg aaatgaacgc gccgaacgcc aaatacggca gccagacctt tgccaggagt
     3181 ttttcgttgc ctgcagacgc gcgtcgcgct ctcagcgagg tgataaggaa aacctgccac
     3241 caggagccaa gaaagaagaa catcacgcca taccggtcga gacggtgaag gagctgcgcg
     3301 agcagcggca gccgatggct ctcgagggta taaatcgtga aaagaaaaag cgccggtatg
     3361 attccgacaa acacataccc gaggacgtcc atgatgaccg ccccggttga ttctttgccc
     3421 gaggtctttc tgtctgtccg cgccgtgacg aagaactcaa atccggccgt gagcggcacg
     3481 atcaggtacg cgagctgctg ataccggtag ctttccatgt aagttccttc ctcccccgat
     3541 agacggaaac gaacaaggtc tggaacccgg caggttgcct acgcagaata ttaatgtttc
     3601 catccccgca tgtcaaagaa aattgcataa aaaatgcgag aaatcggcaa atttttgatt
     3661 tttacatgat gtaatgttat aattagagcc aatgaaacgc gcagccc
//
LOCUS       DASSLD010000680         3980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1615305, whole genome shotgun sequence.
ACCESSION   DASSLD010000680 DASSLD010000000
VERSION     DASSLD010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615305"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VFK23_12325"
     CDS             complement(<1..690)
                     /locus_tag="VFK23_12325"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET7319917.1"
                     /translation="MRNKMSTSLERYRKEISLCVKCGACRAVCPSFLRNREESRSARG
                     RMALVKAVLDGKLAVSDIFRDRLATCTGCLACEASCPSGVPVTDIIQAAKEQAVREAG
                     RGIIAAMLSGMLKRPGLFRTAAWLAPVALHYTKGREKSSEFRVRSSECKAQSLKKKSR
                     GTIAFFPGCAVEHFQPELGAATAGVLNKLGYEVIIPDGLKCCGRPLLSLGDREAAEEL
                     AAHNAGILAALD"
     gene            complement(720..1352)
                     /locus_tag="VFK23_12330"
     CDS             complement(720..1352)
                     /locus_tag="VFK23_12330"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET7319918.1"
                     /translation="MAQNPRNILPLLRLFALLLFAGCLSQPPNAPVPPTGDSSGATSS
                     GAGRHRIVVSKSAYSLTLYRDDRVVKTYRAVFGKGYRDGDKGREGDKRTPEGDFYVCD
                     MHPSKRFYKFLGLSYPSLKHAESGLRSGTISQREYLTIKKALEEGRQPPWDTRLGGAV
                     GIHGRTAATTGVTASSGRENWTDGCIALDNADVDELFSVVSLGTPVTILP"
     gene            complement(1368..1631)
                     /locus_tag="VFK23_12335"
     CDS             complement(1368..1631)
                     /locus_tag="VFK23_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319919.1"
                     /translation="MIRVLLFIAFLIVVYYVMKTVLRAAVRGYHDDEEQRRARLKGEE
                     MVLDPECRTYVVKGRAITRRIDGQLHSFCSEACAKQYEDKKRH"
     gene            complement(1628..2200)
                     /locus_tag="VFK23_12340"
     CDS             complement(1628..2200)
                     /locus_tag="VFK23_12340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7319920.1"
                     /translation="MMTRNLLAVAVLLLALPVACDKHEDGKPSPASRSPQGANKPEDA
                     RNAYYPGVIEEYRTVLAEDPDNLVALIGSGNAYYDSGEWKMAIAMYERALAIDPRNAD
                     VRTDMGTAYRNLGMVERALAEYRLALQHEPGHLNARFNMGIVYANDKKDYTAAIQVWE
                     EILKMAPNYPQAVQIRSMIAALKAKKKAAR"
     gene            complement(2342..3901)
                     /gene="purH"
                     /locus_tag="VFK23_12345"
     CDS             complement(2342..3901)
                     /gene="purH"
                     /locus_tag="VFK23_12345"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET7319921.1"
                     /translation="MAKVTRALISVSNKDGILDFAKRISALGIEILSTGGTAKMLRDA
                     GVKVKDVSEFTGFPEMLDGRVKTLHPKVHGGLLGRRGNPEHVKQMQQHGIEPIDLVVV
                     NLYPFEQTVAKPGCTFEDAIENIDIGGPTMLRSAAKNHTDVAVVVSPRDYGRVLEELR
                     KTGEVSAKTRFELCRTVFLHTARYDSAISVWLDSQVPADEKTRFPNILTLQFEKAQNL
                     RYGENPHQEGAFYREFGRKEPSVAHAKQLQGKEMSFNNFLDANSALELAKEFDGTAAV
                     IVKHNNPCGVAAASTLAEAFRKARDCDPVSAFGGVIAFNRMVDMETARELTATFVEVV
                     VAPEFAPDALAELKKKKDVRLLDIGPSVKGAPEGMDLKKVVGGLIYQDRDLGRIADVR
                     ALRVATKRMPTDDEYDALTFAWKVCKHVKSNAIVFAAKDRTVGIGAGQMSRLDSVRIA
                     KMKAQSPLAGTVLASDAFFPFRDGLDEAAGAGVTAVIQPGGSIKDDEVIRAADEHGIA
                     MVMTGMRHFRH"
BASE COUNT          687 a         1238 c         1205 g          850 t
ORIGIN      
        1 gtcaagtgct gcaagaatcc ccgcgttatg cgcggcgagt tcctccgccg cttcgcggtc
       61 accgagactc aagagcggtc taccgcagca cttcaggccg tccggaatga tcacttcata
      121 tccgagcttg ttcagaacgc cggccgtcgc cgccccaagc tcgggctgaa aatgctcgac
      181 cgcgcacccg ggaaagaacg cgattgtccc ccggcttttt ttctttaagc tttgagcctt
      241 gcactccgaa ctccgaactc tgaactctga acttttttcc cgccccttcg tataatgcag
      301 cgccaccggg gcgagccacg cggcggtgcg gaacagaccc ggacgcttca gcatgccgga
      361 gagcatggct gcgatgatcc ccctgcccgc ttccctgacg gcctgttcct tcgcggcctg
      421 gatgatgtcc gtgaccggca cgccgctcgg acaggaggcc tcgcacgcga ggcatcccgt
      481 gcaggtggca aggcggtccc tgaaaatatc agacacggca agcttgccgt ccaggaccgc
      541 cttgacgagg gccattcttc cccgggcgga ccgggattct tcccggttcc tgaggaagct
      601 ggggcagacg gcacggcagg ccccgcactt cacgcagagg gagatttcct tgcggtatcg
      661 ttccagggac gtgctcattt tattccgcat tccgtactcc gcattccgca tttgtttttt
      721 cacggcagta tcgtcaccgg cgtgccgagg gacacgacgc tgaaaagttc gtcaacgtct
      781 gcgttgtcca gggcgatgca tccgtccgtc cagttctccc tgccggaaga cgcggtaacg
      841 cccgtcgtcg cggctgtcct cccgtggatg ccgacggccc cgccgagccg cgtgtcccag
      901 ggcggctgcc gcccttcctc caatgccttt ttgatcgtga ggtactcccg ctgcgagatc
      961 gttccggacc gcagtccgct ctcggcgtgt tttagcgagg ggtagctcag ccccaggaac
     1021 ttgtaaaacc gtttgctggg gtgcatgtcg cagacgtaga aatcgccttc cggcgttcgc
     1081 ttgtcgccct cgcgcccttt atcgccgtcg cggtagccct tcccgaacac cgcccggtaa
     1141 gtcttgacta cccggtcgtc ccggtagagc gtgagggaat aggccgattt gctgacgacg
     1201 atcctgtggc ggccggcgcc tgatgaggtc gcgccggagg agtcgccggt cggcggcaca
     1261 ggggcattcg gcggctggga caggcagccc gcgaagagga gcagggcgaa aagcctgaga
     1321 aggggaagaa tatttctggg gttttgtgcc atgcgctgtt tacgcggtca gtgccgcttt
     1381 ttgtcttcgt actgtttcgc gcaggcctca ctgcagaacg agtggagttg gccgtcgata
     1441 cgccgggtta tggcccgccc cttcacgacg taggtgcggc attcggggtc gagcaccatc
     1501 tcctcgccct tgaggcgcgc gcgccgttgc tcctcgtcat catggtatcc acggactgct
     1561 gcgcggagca ccgttttcat gacatagtag actacgatca ggaaggctat gaaaagaagg
     1621 acgcgtatca tcgggccgcc ttcttctttg ccttcaaggc cgcgatcatg gacctgatct
     1681 ggacggcttg gggatagttg ggcgccattt tcaggatctc ttcccagacc tgtatagcgg
     1741 ctgtataatc ctttttgtcg ttggcataca cgatccccat gttgaagcgg gcgttcaaat
     1801 ggccgggctc atgctgcagt gccaggcggt actcggcaag cgcgcgttca accatcccga
     1861 ggttccggta ggcggtcccc atgtcggtac gcacatcggc gttccgcggg tcgatcgcga
     1921 gcgccctttc gtacatcgcg atcgccatct tccactctcc gctgtcgtaa taggcgttcc
     1981 cgctcccgat cagggctaca agattgtccg ggtcctcggc gaggacggtc cggtattctt
     2041 cgatcacgcc gggataatag gcgttcctgg catcttcggg cttattggcc ccttgcggcg
     2101 agcgggaagc gggcgagggt ttcccatcct cgtgcttgtc gcacgcgacg gggagcgcca
     2161 gcagaaggac cgccactgca agtaagtttc gcgtcatcat tatttataat ccaggcaggt
     2221 cttgaaaagc catcagccac agaggtcaat gagagcacag aggaaggcga atctgtcgta
     2281 agaactgaac aattgcgggt ttctctgtgt tctctgtggc aaaaaatccc ttcctttttg
     2341 cttaatgcct gaaatgcctc atgcccgtca tcaccatcgc gatcccgtgc tcatccgccg
     2401 cccggatcac ctcgtcgtcc ttgatcgatc cgccgggttg gatcacggcg gtgacccccg
     2461 ccccggcggc ctcgtcgagt ccgtcccgga agggaaagaa ggcgtccgaa gcgaggactg
     2521 tccccgcgag cggggactgg gccttcatct tcgcgatcct gaccgagtcg aggcggctca
     2581 tctgtcccgc tccgatgccg acggtccggt ccttagcggc aaacacgatg gcattggact
     2641 ttacgtgctt gcacaccttc caggcgaagg tgagcgcatc gtattcgtcg tccgtgggca
     2701 tccgcttcgt ggccacgcga agcgcccgga catcggcgat cctgcccagg tcgcggtcct
     2761 gataaatgag gcccccgacg accttcttca ggtccatgcc ctccggggcg cccttgaccg
     2821 acggcccgat gtccaggagc cgcacatctt ttttcttttt cagttccgcg agggcgtcgg
     2881 gagcgaattc cggcgccacg accacttcca caaaggtcgc cgtcagctct cttgcggtct
     2941 ccatgtccac catgcggttg aaagcgataa cgccgccgaa ggccgagacc gggtcgcagt
     3001 ctcgcgcctt gcggaaggcc tcggcgaggg tcgatgcggc tgcgacgccg caggggttat
     3061 tgtgcttcac gatcacggcc gcggtgccgt cgaattcctt cgccagctcg agggcgctgt
     3121 ttgcgtccag gaaattgttg aacgacatct ccttgccctg gagctgtttt gcgtgagcga
     3181 cggaaggctc cttccgtccg aactcccggt agaacgcgcc ttcctggtgc gggttctcgc
     3241 cgtacctgag gttctgagcc ttctcgaact ggagcgtcag gatgttgggg aagcgcgtct
     3301 tttcatcggc cgggacctgg ctatcgagcc acaccgagat cgcgctgtcg tagcgggcgg
     3361 tatggaggaa cacggtgcgg cagagctcga agcgggtctt tgccgagacc tcgccggtct
     3421 tccggagttc ctcaagcacc cggccgtagt cgcgggggga gaccacgaca gccacgtcgg
     3481 tgtggttctt ggccgcggag cggagcatcg tcggcccgcc gatgtcgatg ttctcgatgg
     3541 catcctcgaa ggtgcagccc ggcttggcca cggtctgctc gaagggatag aggttcacca
     3601 ccacgaggtc gatgggctcg atcccgtgct gctgcatctg tttcacgtgc tcggggtttc
     3661 ctcgcctgcc caggagcccg ccgtgcacct tggggtgcag ggttttgacc ctcccgtcga
     3721 gcatttcggg gaaacccgtg aactccgaca cgtccttgac cttgacgccg gcgtcacgga
     3781 gcatcttcgc cgtccctccc gttgagagga tttcgatgcc gagggccgag atcctcttgg
     3841 cgaaatcgag aatgccatcc ttattcgata cactgatgag cgcacgcgtc actttggcca
     3901 cagggagacc tccggaaaaa ttgatgaaaa atccaagcag aatgtgaaga attgtaactc
     3961 aagaggcagg caatgtcaat
//
LOCUS       DASSLD010000681         3181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_285054,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000681 DASSLD010000000
VERSION     DASSLD010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285054"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VFK23_12350"
     CDS             complement(<1..821)
                     /locus_tag="VFK23_12350"
                     /inference="COORDINATES: protein motif:HMM:NF028461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HET7319922.1"
                     /translation="MELFRNRPLKQKFILISLLTTTIAMLVAGGLMFASEFAADRESM
                     VADLTIKAETVGNQCSAALLFNDRKDAEETLSSLRADPDIEYAAVFTRGGALFAQFDR
                     SGKAGRRILQPQAEGYRFEADRLVISRNITLRNRRVGSILISSNLQKFKALLLRYLNA
                     AVIVLSIALGAAYILMSRLQRSITRPVTDLVSMMQGISRHRDFSLRAAETGADELGAL
                     ARGFNEMLSTIQDRDRALEAHRRDLERTISDLETSTEELREANRKLKELDKLKSD"
     gene            complement(823..1383)
                     /locus_tag="VFK23_12355"
     CDS             complement(823..1383)
                     /locus_tag="VFK23_12355"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HET7319923.1"
                     /translation="MKILCHILFCFVLATVVPAVTPGPAFGQPQPGEYEVKAAFLYNF
                     LSFVEWPATVKRSDTLRVCVMAEPPALNAFNELNGQIAAGRKIDAVHLSPGDSPGSCH
                     VLFIGSHAELDLPRIMKAVEGAGVLTVGDTAGFARQGVIINFYLQNKKVRFEINAAAA
                     RRSGITISSKLLKLAGAVYGAAPAGE"
     gene            complement(1574..>3181)
                     /locus_tag="VFK23_12360"
     CDS             complement(1574..>3181)
                     /locus_tag="VFK23_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7319924.1"
                     /translation="AFVGTEAIIDTDLIDRVEIVRGPGSSLYGSNAFFAVVNIVTKRG
                     RDVKGVEISGDAGSFNTSKGRLTYGGRSQNSLEAVASGTAYDSRGRSLYFPEFDPAIS
                     TDPRAANGGISDRSDYDRSQSFFAKAAYRDFTFEGVYAERTKGIPTGAFNTNFNDPST
                     KTTDGHSYLDVRYDHDLSGRTSLMARAYADYYWYRADYVTGDVVNKDFGYGDWGGGEI
                     KISSRFFNDHRVIAGAEYTDNIRQDQRNYNVIPYDLILDDKRKSRIWASYVQDEFPLA
                     GNVIVNAGVRYDHYSTFGGTTNPRLAFIYEPTARSAVKLIYGSAFRTPNVFELYYSTP
                     GTNVPNPNLKPEKIKTYELVYELYAGQGFRVSASGYYYRINDLINQVETGAGTKFENV
                     DEVTARGAGLELENKWPNGVDGRFNWSIQRTRDKLTGEQLTNSPEQLAMLNLSVPVVN
                     NKVFASVEERYMSRRRTEGGNNTQDFFITNLTLFTQNLMRRLELSASVYNLFGKQYSD
                     PVSLDLLPLDTVRQDGRSYRVKATYLF"
BASE COUNT          622 a          931 c          926 g          702 t
ORIGIN      
        1 aaatcagatt tcagcttgtc gagctccttc agttttctgt tcgcttcccg cagttcctcg
       61 gtcgaagttt cgaggtccga gatcgtccgc tctaggtcgc gccggtgcgc ctcaagcgcc
      121 cggtcccggt cctggatcgt cgaaagcatc tcattgaacc ccctggccag ggcgcccagt
      181 tcatcggcgc cggtctcagc ggccctgagc gagaaatccc tgtgccggga tatgccctgc
      241 atcatcgaca cgaggtccgt gacggggcgc gtgatggacc gctgaagacg cgacatgaga
      301 atataggctg ccccgagcgc gatggagagg acgatcacgg cggcgttaag gtagcgcagc
      361 aggagcgctt tgaatttttg aaggttcgag ctgataagga tcgacccgac ccgtcgattg
      421 cgcagggtga tgttccggga gataaccaga cggtcggcct cgaatcggta tccctcggcc
      481 tgcggctgaa ggatccgccg gccggccttt cccgaacggt cgaactgggc gaaaagggcc
      541 cctcctcggg tgaacaccgc ggcatattca atgtcggggt ccgcccgcag cgatgaaagc
      601 gtttcctccg cgtccttccg gtcgttgaac aggagcgccg cggagcactg attgccgacg
      661 gtctcggcct tgatcgtcag gtcggcgacc atggactccc ggtcggcggc gaattcggag
      721 gcaaacatga ggccgccggc caccagcatt gcgatcgtcg tggtcaggag actgatgagg
      781 atgaacttct gtttgagcgg acggttccgg aagagctcca tatcattccc ccgccggcgc
      841 cgcgccgtag accgcgccgg cgagcttcag aagtttggag ctgatcgtga tgcccgaccg
      901 ccgggccgcg gctgcgttga tctcgaaacg gacctttttg ttctgcagat agaaattgat
      961 gatgacgccc tgcctggcaa aacctgcggt gtcgcccacg gtgaggacgc cggcgccctc
     1021 gacggccttc atgatcctgg gaaggtcgag ttcagcatgg gagccgataa agagaacatg
     1081 acatgatcct ggactgtcgc ccggcgacag gtggacggcg tcgatcttcc ggcccgccgc
     1141 gatctggccg ttcagttcgt tgaacgcgtt cagggcaggc ggctcggcca tcacgcacac
     1201 tcgcagcgtg tctgagcgtt tcaccgtcgc cggccactcc acgaaggaaa gaaaattgta
     1261 gagaaatgcc gctttcacct cgtactctcc cggctgaggc tggccgaagg ccgggcccgg
     1321 agtaacagcc ggaacaactg tggcaagtac aaagcaaaac agtatatgac agagaatctt
     1381 catgaggtgt ttactcagat ctatcacgaa tgctcgcaca gcaacgtaaa accagttcgt
     1441 cattcccgga tgcttttatc gggaatccag attccttagc attttttgta tcgctaaaga
     1501 tcctggattc ccgctggagc ctgccccagc atgatttaag ccggggcggg aatgacgatc
     1561 tgagcaatta cgattaaaac agatacgtcg ctttcacccg gtagctccgg ccgtcctgcc
     1621 gtacggtgtc gagcggcaga aggtctaagg ataccgggtc gctgtattgc tttccgaaaa
     1681 gattatatac gcttgccgac agttcgagcc tgcgcataag gttctgggtg aaaagcgtca
     1741 gattggttat gaaaaagtcc tgcgtgttat tgcctccctc ggtccttctc cggctcatgt
     1801 atcgctcttc gacgctcgcg aagaccttgt tattcacgac cggcaccgaa aggttcagca
     1861 tcgcgagttg ttcgggagag ttcgtgagct gctcgccggt cagtttgtcc cgagtccgct
     1921 ggatgctcca gttgaaccgt ccgtcaaccc cgttcggcca cttgttttcc agctccagcc
     1981 ccgctccccg cgccgtcacc tcgtccacgt tctcgaactt cgtccccgct cccgtctcca
     2041 cctggttgat caggtcgttg atccggtagt agtagcccga ggcgcttacg cggaatccct
     2101 gacccgcata cagttcatag accagctcat aggttttgat cttctcgggt ttgaggttcg
     2161 ggttgggtac gttggtcccg ggggtcgaat aatagagctc gaacacgttc ggcgtccgga
     2221 aggcgctgcc gtagatcagt ttgacggcgc tcctcgcagt cggttcatag atgaatgcca
     2281 gcctggggtt cgtggtaccg ccgaaggtgc tgtagtgatc atagcgcacg cccgcgttca
     2341 cgatcacgtt tccggcgagc gggaattcat cctgaacgta ggacgcccag atcctggact
     2401 tccttttgtc gtcgagaatg agatcatagg gaatgacgtt gtaattcctc tggtcctggc
     2461 ggatattgtc cgtatactcg gccccggcga tcacccggtg atcgttaaaa aaacgggaag
     2521 aaatcttgat ctcaccgccc ccccaatcgc cgtatccgaa gtccttgttt acgacgtctc
     2581 cggtgacgta atccgcccgg taccagtagt aatcggcata ggccctcgcc atgagacttg
     2641 tcctgccgct caggtcatga tcgtatctta catccaggta agaatgaccg tccgtcgttt
     2701 ttgtcgacgg gtcattgaag ttggtgttga aagctcccgt gggaatgccc ttcgtccttt
     2761 ctgcatacac gccctcgaac gtaaaatcac ggtacgccgc ttttgcgaag aagctctggg
     2821 agcggtcata gtctgaacgg tccgatatgc cgccgtttgc cgcgcgcggg tcagtggaaa
     2881 tcgcgggatc gaactccggg aaatacaggc tccgccccct gctgtcgtag gccgtgccgg
     2941 aagcgaccgc ttccaggctg ttctgagagc ggcccccgta agtaagtctc cccttggagg
     3001 tattgaaact gcccgcatcg cccgaaattt caactccctt cacatcccgg ccccgcttcg
     3061 tgacgatgtt cacgaccgcg aaaaaggcgt tgctgccgta cagggaggac ccggggccgc
     3121 gtacgatttc cacgcggtcg atgaggtctg tgtcgatgat ggcctcggtc ccgacaaagg
     3181 c
//
LOCUS       DASSLD010000682         3703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_69921,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000682 DASSLD010000000
VERSION     DASSLD010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69921"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFK23_12365"
     CDS             <1..258
                     /locus_tag="VFK23_12365"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HET7319925.1"
                     /translation="ANRYVVDGRTDIGKVNEILGVQLPEDEFETIGGFVLGLFGRLPA
                     EGDQIRYNNLLFTVLRLGKNRISRVRVLKYAPENQEPENES"
     gene            393..1277
                     /locus_tag="VFK23_12370"
     CDS             393..1277
                     /locus_tag="VFK23_12370"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET7319926.1"
                     /translation="MTTPLVLETNLEGLKPPRRGKVRDVYDLGDKLLIVGTDRISAFD
                     VVLPNAVPEKGRVLTQISKYWFAQTEGIVKNHVISTAVKDFPPVCKPHAPVLEGRSML
                     VKKTQPLPVECVVRGYLTGSGLKEYKAAGSVSGVKLPAGLTEASRLPEPIFTPSTKAE
                     VGEHDENIDFDSVIKLVGKETAEKIKSYTISIYKKACELAEPKGIIIADTKLEFGIYQ
                     GEVILIDEVLTPDSSRFWPKNDYKVGVAQKSFDKQFVRDYLLSLAWNQKPPAPVLPEE
                     VVRKTSEKYLEVLRMLTN"
     gene            1386..2156
                     /locus_tag="VFK23_12375"
     CDS             1386..2156
                     /locus_tag="VFK23_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319927.1"
                     /translation="MKKIVIAVLLGVSLFNPSSVLAADIEDGFMRGIFQYLAKRRSMN
                     LVAYSGEKVAEIGRLYYVEEPDTPGKLKNWASHGKEIKLFEYSVGYNLSEHVEYQKEE
                     SYQIQKQYADYLAAELKARLKEQGVELEAFKKALGKAQIRFTVYRKVVEARLVSNAVQ
                     ADKQNIIKEFDAFEQGEGLLIPFQQLVITDFSYNEDAASELNALLGYELLKTVKAKLS
                     ANMIKTQDAKVDLPASSTIAFKAFPVYFKPTHFFGMWF"
     gene            2447..>3703
                     /gene="lysS"
                     /locus_tag="VFK23_12380"
     CDS             2447..>3703
                     /gene="lysS"
                     /locus_tag="VFK23_12380"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET7319928.1"
                     /translation="MEDYNELIQQRFKKLAEISASGVKPYAGRFEVTASANGLAEKYG
                     QASKEDLEKERIEVTVAGRIIAMRSFGKACFSHVQDGSGRVQLYFQKNTLGEDRYGLF
                     KKLDIGDFIGAKGFLFRTKTNELTIDVLDFTLLSKSLRPLPEKWHGLTDVELRYRQRY
                     VDLIVNPEVKSVFVLRTKIVQAIRIFLNTRGYLEVETPMMQTIPGGATAKPFKTHHNA
                     LDMDLYLRIAPELYLKRLLVGGFERVYEINRNFRNEGISTRHNPEFTMLEFYTAYADY
                     RVLMDMTEQMISTVAREVRGTTEIEYEGHKISLTPPWKRITYMDALREAGVPEDVLTD
                     PGKAEAHARSLGASLKGGEPLGKVLNEIFEALVEPNLIQPTFVTDYPTDISPLSKRRE
                     DNPAFVERFELFVVGRELANAFSELND"
BASE COUNT          965 a          965 c         1012 g          761 t
ORIGIN      
        1 gcgaaccggt atgtcgttga cggcaggacg gacatcggaa aagtgaatga aatactgggt
       61 gtgcagctcc ccgaggacga atttgagacc atcggcggct ttgtgctggg actcttcgga
      121 agactgcctg cagagggaga ccagatccgg tacaacaacc ttctgtttac cgtgctccgg
      181 cttggaaaaa accgcatctc ccgagttcgg gtgctgaaat acgcgccgga aaaccaagag
      241 cctgaaaacg aaagttgaaa ccactggtcc cgttaaaacg cgggacacac ggttaaagtc
      301 agaattaaac tttaagaact gtgtaatccg tgggttctgt cgaagacgga acccgtggtt
      361 ccatgccgtt tcaaatgatg aaagaggtga caatgacgac accgctggta cttgagacca
      421 atctggaagg actgaagcct ccccgccggg gaaaagttcg cgacgtctac gaccttggag
      481 acaaacttct catcgtcggg acggacagga tctctgcctt cgatgtggtc ctgccgaacg
      541 ccgttccgga gaaggggagg gtcctgaccc agatctcgaa atactggttt gcgcagactg
      601 aggggatcgt aaagaaccat gtgatctcta cggcggtaaa ggactttcct ccggtatgca
      661 agccgcacgc gcccgtcctc gaagggagga gcatgctcgt aaaaaaaaca cagccccttc
      721 ccgttgagtg cgttgtccgc ggctatctca ccggatccgg tctgaaggaa tataaggcag
      781 ccggctcggt cagcggcgtc aagctgcccg cgggcctgac cgaggcgtcc cggctgccgg
      841 agccgatctt taccccctcg accaaggcgg aagtcggaga gcacgacgag aacatcgatt
      901 tcgattcagt gataaagctt gtgggcaaag aaacggcgga aaagatcaag tcctatacga
      961 tttcgattta taaaaaggcg tgcgaactcg cggagccgaa gggcatcatc atcgcggaca
     1021 cgaaactcga attcggcatc tatcagggag aggtcatcct aatcgacgag gtgctcacgc
     1081 ccgattcctc ccggttctgg ccgaagaacg actataaggt cggcgtcgca caaaaaagct
     1141 tcgacaagca attcgtacgc gactaccttc tctccctggc gtggaaccag aagccgccgg
     1201 ccccggtcct tcccgaagag gtcgtcagaa aaacgagcga aaagtacctg gaagtgctca
     1261 ggatgctgac gaactagcgg aggaggtccg atgtgtctgt ttttgaatcg gcgccctgaa
     1321 cgatgggaag ggtcctgcat cggtccggat agcgaaacgc gtatgactct ctatcggaga
     1381 gaccagtgaa aaaaattgtc attgcggttc ttcttggcgt ttccctgttt aacccgagtt
     1441 cggttttagc cgccgatatt gaagacggat tcatgcgcgg aatttttcag tatctggcaa
     1501 aacgcaggag catgaatctg gtggcataca gcggagaaaa ggtggccgag atcggtcgtc
     1561 tctattatgt ggaagaacct gatacgcccg gcaagctgaa aaattgggcg tcgcacggca
     1621 aggaaataaa actttttgaa tatagcgtcg gatacaatct gtccgaacac gttgaatatc
     1681 aaaaagaaga aagctaccaa atccagaaac aatacgccga ttaccttgcc gcagaactta
     1741 aggcccggct taaagagcaa ggggtcgaac ttgaggcatt taaaaaggcg ttagggaagg
     1801 cgcaaattcg atttaccgtt tatcggaaag ttgttgaggc aaggctggtt tcgaatgccg
     1861 tacaagccga taaacaaaac atcatcaaag aattcgatgc gttcgaacaa ggagaggggc
     1921 tcctcattcc atttcaacaa ttagtcataa cggatttttc gtacaacgag gacgccgcaa
     1981 gtgaattaaa cgcgctgctc ggatatgaat tactgaaaac cgttaaagcc aaactgtcgg
     2041 caaatatgat taaaacccag gatgcaaagg tggatttacc ggcttcatca accatcgcgt
     2101 tcaaggcctt tccggtgtat ttcaagccca cgcatttttt cgggatgtgg ttttaaaaag
     2161 acttatcccc acccaatatc gtttctcaca taacgaacag ctatggtctc acttatgtcc
     2221 gaacctgatt gtccttgacg attccgcgtt taatcagtat aatccgcgat tattcattct
     2281 aagaagtatt tggacgcaga tactaaacga aattggatag acgcaggtca aaaccagtcg
     2341 ataatcgttg agaaatgaaa aaagaaccaa ttgtatgtct ggttctatct gcgtaaatct
     2401 gcgtttatca gcgtccaatt accgtttcag atcgaaagga agcagcatgg aagactacaa
     2461 cgagctcatt cagcagcggt tcaagaagct cgcggaaatc agcgcgagcg gtgtaaagcc
     2521 gtatgccgga aggttcgagg tcaccgcctc agcgaatggg cttgccgaaa aatacggtca
     2581 ggcgagcaag gaagacctcg aaaaagagcg catcgaggtc acggtggccg gcaggatcat
     2641 cgccatgcgg agcttcggca aggcgtgctt ttcccacgtt caggacggct cgggcagggt
     2701 ccagctctat ttccagaaga acaccctcgg cgaagaccgc tacggcctgt tcaagaagct
     2761 cgacatcggc gatttcatcg gggccaaggg gtttctcttc cgcacgaaga cgaatgaact
     2821 gaccatcgac gtcctggatt tcacgctcct gtccaagtcg ctcaggccgc ttcccgaaaa
     2881 gtggcacggt ctcaccgacg tcgagctccg ctaccgccag cggtatgtcg atctcatcgt
     2941 caatcccgag gtcaagagcg tctttgtcct gcgcacgaag atcgtgcagg ctatacggat
     3001 ctttctgaat acccgggggt acctggaggt ggagacgccc atgatgcaga ccatccccgg
     3061 cggcgccacg gcaaagccgt tcaagaccca tcacaacgcc ctggacatgg acctttatct
     3121 ccgcatcgcg cccgaactgt atctgaaacg gctcctggtg ggcggcttcg aacgcgtcta
     3181 tgagatcaac cggaacttcc ggaacgaggg catatccacg aggcacaatc ccgagttcac
     3241 aatgctggag ttctacaccg catacgccga ttaccgggtc ctcatggaca tgacggagca
     3301 gatgatcagc accgtggcgc gggaggtccg cggcacgacc gagatcgagt acgaaggcca
     3361 caagataagc ctcaccccgc cctggaaacg gattacctat atggatgcat tgcgggaagc
     3421 gggggtgcct gaagatgtgc tcacggaccc gggcaaggcg gaggcgcatg cccgaagcct
     3481 cggtgcatcg ctcaagggcg gggaacccct gggcaaggtg ctgaacgaaa tcttcgaggc
     3541 cctcgtggag cccaacctga tccagccgac ctttgtcacc gattatccca cggacatctc
     3601 tccgctttcc aagagacgcg aagacaaccc cgctttcgtc gagcggttcg agctgttcgt
     3661 ggttggccgc gagctcgcga acgccttctc ggaactgaac gat
//
LOCUS       DASSLD010000683         2619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1124565, whole genome shotgun sequence.
ACCESSION   DASSLD010000683 DASSLD010000000
VERSION     DASSLD010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124565"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VFK23_12385"
     CDS             complement(<1..363)
                     /locus_tag="VFK23_12385"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HET7319929.1"
                     /translation="MFILLFSYLLVLGCRFWLLSLNLGYLKKHGKTVPPEFEGVIEPD
                     RLGRISAYTFEKSRIGIVESVISSAATIIFFFGGILGSYDRWIGSMSGSFLSGGLIFF
                     LPLLYAELLIDIPFSLYKN"
     gene            complement(860..1459)
                     /locus_tag="VFK23_12390"
     CDS             complement(860..1459)
                     /locus_tag="VFK23_12390"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7319930.1"
                     /translation="MQTVSSLLRREGDLEVVSTVSSRESAIKQLSNTPDVLIIDPEIL
                     KGHTLSRFIRSVQTRSPHTRTMYLFGKVPPDESVIADIKTGIRGYMKTSDAPQTMIKA
                     IRAVHGGEIWAERRILEKAIEKPMLLPETLQTHVPGLPPLTNREIEVLTMVLQGASNK
                     EIADSNSISERTVKTHLYRVYRKLNVKSRTKAIALLAHS"
     gene            complement(1665..2246)
                     /locus_tag="VFK23_12395"
     CDS             complement(1665..2246)
                     /locus_tag="VFK23_12395"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET7319931.1"
                     /translation="MKNHAPIILASASPRRNSLLRQFGVPFKTVPADVDERVRAGETP
                     EQYARRLSLDKARAASDKAGKGIVIAADTIVVLDDEILGKPSDERDALRMLSMLSGRT
                     HRVITALTVMDAAGKILTRLSQTIVRFRRLSAEEIMSYVKTGEPLDKAGAYGIQEKGA
                     LLVSGIEGCYFNVVGLPISLLGEMLKEFGVNML"
     gene            complement(2290..>2619)
                     /locus_tag="VFK23_12400"
     CDS             complement(2290..>2619)
                     /locus_tag="VFK23_12400"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HET7319932.1"
                     /translation="RVTPFDGVVCGDSVTLEREIRNMPPVITAHNEFSFDGTVYSYQV
                     KASDPDDDTLAYSLEGAPDGMAINKSTGLITWKVPSGFKGDAGATAVVNDGNGGVARY
                     SLKISIK"
BASE COUNT          625 a          669 c          693 g          632 t
ORIGIN      
        1 attcttgtag agactgaagg ggatgtcaat gagcaattcg gcgtacagga ggggtagaaa
       61 gaagatgagc cctcctgaca ggaaagatcc ggacatcgat ccgatccagc ggtcgtaaga
      121 gccgaggatg ccgccgaaga aaaaaatgat cgtggctgca ctgctgataa ctgactcgac
      181 aataccgatc ctgcttttct cgaacgtata cgccgatatc ctgcccaggc gatcgggttc
      241 gatcacccct tcgaattcag gcggcacggt cttaccatgt ttcttcaaat aacccaggtt
      301 cagggaaaga agccaaaagc ggcatccgag aacgagaagg taggaaaaaa gcagaatgaa
      361 cataggttca gaaatgggtt cacagggtca ctagtttgtc attcccgaat aagtcctaaa
      421 gcctgccccg gtatgattga agccaagggg aaatccattt tgagtttagc ttggaaaagc
      481 cactggattc ccgctggagt ctgccccagc atgattcaag ccggggcggg aataacaaat
      541 tgtggtgagt aaaaaaaaca aggcgtcccg atgtccttga cagagggatt cccctcgtcg
      601 gtgcggcacc gagacgcctc tgcagaccac gcctgtcagt tgctgttaac ctccctgatg
      661 gagatccact ctcgtccgtg acatcatggt gtcttctctt cacgcggcgt aaaccgggtt
      721 ggggggggaa gattttagcc gcggttgtcg tagccaccgg acggtgagtt ccgacaggcg
      781 tagtctttac ctctagaata acaactaggg atgttaagtc aatacatcga aagttgtatt
      841 ctctggaaga acaaaaagtt tacgaatgtg ccagcagggc aatcgccttt gtcctgcttt
      901 taacattcag tttcctgtag acccggtaga gatgggtctt caccgttctt tcgctgatgc
      961 tgttgctgtc cgcaatttcc ttgttcgatg ctccctgcaa taccatggta aggacttcta
     1021 tttccctgtt cgtcaagggc ggaaggccag gcacatgggt ttgcagtgtt tcaggcagaa
     1081 gcatcggctt ttcgatggcc ttttccagaa tcctgcgttc ggcccagatc tctccaccat
     1141 gaaccgcgcg gatcgccttg atcatcgttt gcggcgcatc ggatgtcttc atgtatcctc
     1201 tgatcccggt cttgatgtct gcaatgacgc tttcatccgg cggaaccttt ccaaaaagat
     1261 acatggtcct cgtatgaggg gaccgcgtct ggaccgatct gataaagcgc gaaagcgtat
     1321 ggcccttcag gatttccgga tcgattatca gcacgtcagg cgtgtttgac agttgcttta
     1381 ttgcggattc ccgcgaagaa acggttgaaa ccacctcgag atcaccttcc cggcgcagga
     1441 gcgacgatac tgtttgcatt tcagccgcgt cgcctacggc tatcatgaca cgaataggta
     1501 acactttaga tacctccata gggaattgca atctttctaa acaatttctc taatactatt
     1561 ttaagacgag gctaagagta tgtcaagccg gggagaaaag aattaggtgt ttcaagggta
     1621 gggagaagta atcacttatg aacaattttg acgttatctt gattttacaa catgttgaca
     1681 ccgaattcct tcagcatctc gcccagaagc gatatcggga gcccgacgac gttaaaatag
     1741 cagccttcga tgccgctgac gagaagagcg cctttttcct ggatgccgta ggctccagct
     1801 ttgtcgagcg gctctccggt tttgacatag gacatgattt cctctgcgga aagccggcga
     1861 aaccgtacga tagtctgaga caacctggtc aggatttttc cggcggcgtc cataaccgta
     1921 agcgcggtta tgacgcggtg ggtccttccc gacagcatgg agagcatgcg tagggcatcc
     1981 cgctcgtctg agggttttcc gaggatttcg tcatccagta cgacaatcgt gtccgcggcg
     2041 atgacgatcc ccttgccggc cttgtcagag gccgcgcggg ctttatcgag ggacaggcga
     2101 cgggcgtatt gttccggcgt ctcgcccgcg cggacacgct cgtccacatc tgcgggtact
     2161 gtcttaaatg gtacgccgaa ctggcgcaga agactgtttc ggcgcggaga ggctgaggca
     2221 aggataatcg gtgcgtgatt tttcatgtct gagacgacac aagatgcggc aaaacaggca
     2281 gaaagaatac tacttgattg atattttcag actgtaccgc gcaactccgc catttccatc
     2341 attgaccacg gctgttgcac cggcatctcc cttgaaaccc gaagggacct tccacgttat
     2401 caggccggta gacttgttaa tcgccatgcc atccggcgcc ccctccagag aatacgcaag
     2461 ggtgtcatca tccgggtcgg acgcctttac ctgataggaa tacactgtcc cgtcgaaact
     2521 aaattcattg tgggctgtta taacgggcgg catgtttcgt atctctcgct caagggtgac
     2581 cgaatcgccg caaaccaccc cgtcaaaggg cgtgactct
//
LOCUS       DASSLD010000684         5127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_930380,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000684 DASSLD010000000
VERSION     DASSLD010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930380"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2979
                     /gene="rpoB"
                     /locus_tag="VFK23_12405"
     CDS             <1..2979
                     /gene="rpoB"
                     /locus_tag="VFK23_12405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET7319933.1"
                     /translation="EKMEVIYMDGIHVIAAMRDTLLSDKVQTSDEALLEIYRKMRPGE
                     PPTIDMARNLFTGMFFNAKRYDLSPVGRLKLNKKLKLDIPIEKTTLTAQDVVEVIRYL
                     VSLRTGRGEVDDIDHLGNRRVRSVGELLENQIRVGLARMERAIKERMSLQDIDTLMPH
                     DIINPKPVIAAIKEFFGSSQLSQFMDQTNPLAEITHKRRLSALGPGGLTRERAGFEVR
                     DVHTTHYGRICPIETPEGPNIGLIVSLATYARVNDYGFIEAPYRKVKAGKVSDEIEYL
                     SAVDGENYVIAQANTPVDGRGKIAVESVSARVGGEFKLVSAEEVQYMDVSPKQIVSVS
                     AALIPFLENDDANRALMGSNMQRQAVPLLRPQAPVVGTGMERVAARDSGAIVSAKRDG
                     IVEGVDSTRITVKGEDAKGRTVIDTYPLTKFYRSNQNTCMNQKPIVRIGDKVKKGQVI
                     SDGPATDLGELALGQNVLVAFMPWSGYNYEDAIIISEKLVKEDRYTSIHIEEFEIEAR
                     DTKLGKEEITRDIPNLGEETLRNLDENGIIRIGAEVLPGDILIGKVTPKGETQLTPEE
                     KLLRAIFGEKAGDVRDASLYAPPGIEGTVIDVKVFSRKGEDKNERLKTQESEEIARLQ
                     KEYQDEMRAVKEDKYKRIRRVLAGKTAADKVVNSKTGKTELAKGKKITAEILDKLPEK
                     DLAKIKVESEDYPAKLQEILDAAENQLSILESIYDEKIGRLKRGDDLPPGVVKLVKVY
                     VAMKRKLSVGDKMAGRHGNKGVVSRIVPEEDMPYLPDGTPVEIVLNPLGVPSRMNVGQ
                     ILETHLGWAAKALDLHVATPVFDGASEDRIKAMLKEAKLPITGQSQLFDGRSGDPFDK
                     PVTVGYMYMLKLHHLVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWALQAY
                     GAAYCLQEFLTVKSDDVSGRAKIYEAIVKGENTLEPGLPESFYVLIKELQSLCLDVEL
                     IERKNEKERVIGLEKISSAR"
     gene            3155..>5127
                     /gene="rpoC"
                     /locus_tag="VFK23_12410"
     CDS             3155..>5127
                     /gene="rpoC"
                     /locus_tag="VFK23_12410"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET7319934.1"
                     /translation="MRIKIASPDRIKEWSYGEVKKPETINYRSFKPERDGLFCAKIFG
                     PIKDWECLCGKYKRMKHRGIVCDKCGVEVIQSKVRRERMGHIELSSPVAHIWFLKGLP
                     SRIGTLLDMTLRQLEKVLYFEAYVVVEAGDTPLKYKELLTEEKFRKAQQEYGVGAFKA
                     GMGAEAIREMLKVIDLETESEDIRKKIETATSMATKKKLTKRLKVIEAFRKSGNRPEW
                     MILDVIPVIPPELRPLVPLEGGRFATSDLNDLYRRVINRNNRLHRLEELKAPSVIIRN
                     EKRMLQEAVDALFDNGRRGRVLRGPNKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGR
                     SVIVVGPDLKLHQCGLPKKMALELFKPFIFNKLEEKGYATTIKSAKKMIERERPEVWD
                     VLEEVIQEHPVLLNRAPTLHRLGIQAFDPVLVEGKAIRLHPLVCAAFNADFDGDQMAV
                     HIPLSVEAQLEARVLMLSVNNILSPANGRPITVPSQDIVLGCYYLTKARSGVLGEGKI
                     FSGRDEVRIAYDQGVADIHARIRVRINGKIEETTVGRVLFSDILPAGMSFSSVNKEMT
                     KKELAKLIDECYRKLGLRRTVILIDDLKEMGFKYATRAGISICISDMHIPGKKQKFID
                     QAQKEVMEIHHQYTEGLITNGERYNKEIDIWANV"
BASE COUNT         1197 a         1490 c         1571 g          869 t
ORIGIN      
        1 gagaagatgg aagtgatcta catggacggc atccacgtga tcgctgccat gcgcgatacg
       61 ctcctgtccg acaaggtgca gacgtcggac gaagcactcc ttgagatcta ccggaagatg
      121 cgtcccggag aaccgccgac catcgacatg gcgcggaacc tgttcaccgg catgtttttc
      181 aacgccaagc gctacgatct gtcgcctgtc ggcagactca aattgaacaa gaagctcaag
      241 ctcgacatcc cgatcgagaa gaccacgctg accgcccagg acgtcgtgga ggtgatccgc
      301 tatctggtca gccttcgaac cggcaggggc gaggtggacg atatcgacca tctcggcaac
      361 cgccgggtcc gctccgtggg cgaactcctc gagaaccaga tccgcgtcgg ccttgcccgc
      421 atggaacgcg ccatcaagga acgcatgagt cttcaggaca tcgacaccct gatgccgcac
      481 gacatcatca atcccaaacc ggtgattgcg gccattaaag agttcttcgg gtcgagccaa
      541 ctctcacagt tcatggacca gacgaacccg cttgccgaga tcacgcacaa gcgcaggctc
      601 tcggccctgg gccccggcgg ccttacccgg gagcgcgcag ggttcgaggt gcgcgacgtg
      661 cataccacgc actacggcag aatatgcccc atcgagacgc cggaaggccc gaatatcggc
      721 ctgatcgtat cgcttgcgac ctatgcgcgg gtcaacgact acgggttcat cgaggcgccc
      781 taccggaagg tcaaagccgg caaagtgtcc gacgaaatcg agtacctttc cgccgtggac
      841 ggggaaaatt acgtcatcgc ccaggcgaac acacccgtgg acggccgcgg caagatcgcc
      901 gtggagagcg tgtccgcccg tgtgggcggc gaattcaaac tggtatccgc cgaggaagtg
      961 cagtacatgg acgtgtcgcc gaaacagatc gtttcagtct ctgcggccct gatccccttc
     1021 ctcgaaaacg acgacgccaa ccgcgcgctg atgggctcca acatgcagcg ccaggccgtg
     1081 ccgctcttgc ggccgcaggc gccggtcgtc ggcaccggca tggagcgcgt ggccgcccgc
     1141 gattcgggcg ccatcgtttc cgccaagcgc gacggcatcg ttgagggcgt agactccacg
     1201 cgaatcacgg tcaagggcga ggacgccaag ggccggacgg tcatcgatac ctacccgctc
     1261 accaagttct accgctcgaa ccagaacacc tgcatgaatc agaagccgat cgtcaggatc
     1321 ggcgacaagg tgaaaaaggg gcaggtgatc tccgacgggc cggccacgga cctgggagaa
     1381 ctcgccctgg gtcagaacgt gcttgtggcg ttcatgccct ggagcggata caactacgag
     1441 gacgcgatca tcatcagcga aaaactggtc aaggaggacc gctatacctc gatccatatc
     1501 gaggaattcg agatcgaggc ccgcgacacc aagctcggca aggaagagat cacccgcgat
     1561 atcccgaacc tcggcgagga aaccttgagg aacctcgacg agaatgggat catccgcatc
     1621 ggcgccgagg ttcttcccgg ggacatcctg atcggcaagg tcacgcccaa gggcgagacg
     1681 cagctcacgc ccgaggaaaa actcctgcgg gccatcttcg gcgagaaggc aggcgatgtg
     1741 cgggacgcct ctctctatgc gcctccgggc atcgaaggaa cagtcatcga cgtcaaggtt
     1801 ttttcgcgca agggcgagga caagaacgag cgcctcaaga cgcaggagag cgaggaaatc
     1861 gcacggctcc agaaggaata ccaggatgaa atgcgcgcgg tcaaagagga caaatacaag
     1921 cgcatccggc gggtcctggc cggcaagacg gccgctgata aggttgtcaa ctccaagaca
     1981 ggcaagacgg agctggcgaa ggggaaaaag atcaccgctg agatcctcga taaactaccc
     2041 gaaaaggatc tggccaagat caaggtggag tccgaggact atcctgccaa gctccaggag
     2101 attctggacg ccgccgagaa ccagttgagt atactggagt ccatctacga cgaaaagatc
     2161 gggaggctga agcgcggtga tgaccttcca cccggcgtgg taaagctcgt caaggtctac
     2221 gtggccatga agcgcaagct ctccgtcggc gataaaatgg cgggcaggca cgggaacaag
     2281 ggcgtggttt cccggatcgt gccggaagag gacatgccgt accttcccga cggaacgccg
     2341 gttgagatcg tgctgaaccc gctgggcgtg ccatcccgta tgaacgtggg acagattctg
     2401 gagacgcacc tgggctgggc cgccaaggcc ctcgacctgc acgtggctac tccggtgttc
     2461 gacggggcct cggaggatcg gatcaaggcg atgctcaagg aggcgaaact gccgatcacg
     2521 ggccagtcgc agctcttcga cggccggagc ggagatccct ttgacaagcc ggtgaccgta
     2581 gggtacatgt atatgctgaa gctccatcac ctcgtcgacg acaagctgca tgcccgctcc
     2641 atcggacctt attcgctggt gacgcagcag cccctcgggg gcaaggccca gttcggcggt
     2701 cagcgcctgg gcgagatgga agtctgggcc ctccaggcct acggggccgc ctactgtctg
     2761 caggaattcc tcacggtgaa gtccgatgac gtctccggac gcgccaagat ctacgaagca
     2821 atcgtgaagg gcgagaacac gctggaaccg gggcttcccg agtcgttcta cgtgctcatc
     2881 aaggagctgc agagcctctg tctcgacgtg gaactcatcg agcgtaagaa cgagaaggag
     2941 cgggtgatag ggttggagaa gatttcgtcg gcaaggtaga gagtgaataa cgggtcttgt
     3001 aggtcgtacc ggtcctataa gatctcttgg acttgggatg tagtgctttt gcacacggag
     3061 gttcacggtg gaagacaaac agatatcgga aaataaggcg cgggcggagg ttctcagcct
     3121 cttcgagaaa ccgaaggaga tcagcttcga cgcgatgagg atcaaaatcg cgtctccgga
     3181 ccggatcaag gagtggtcct acggcgaggt caagaaaccg gaaacgatca attaccggtc
     3241 tttcaaaccc gagcgtgacg gcctgttctg cgcgaagatt ttcggcccga tcaaggattg
     3301 ggagtgtctc tgcggtaagt acaaacggat gaagcaccgc ggtattgtgt gcgacaagtg
     3361 cggcgtcgag gtgatacaat ccaaagtgcg gcgcgagcgc atgggccaca tcgaactttc
     3421 ctcaccggtc gcacacatct ggttcctcaa gggactcccg agccgcatcg ggacgctcct
     3481 cgacatgacg ctcaggcagc tcgagaaggt cctctatttc gaggcgtatg tcgtcgtcga
     3541 agccggcgat acgccgctca aatacaagga actcttgaca gaagaaaaat tccgcaaggc
     3601 gcagcaggaa tacggcgtgg gcgcgttcaa ggcgggcatg ggcgccgagg cgatccggga
     3661 gatgctcaag gtcattgacc ttgaaaccga gtctgaagat atccggaaaa agatcgagac
     3721 cgcgacctcc atggcaacga agaaaaagtt gaccaaacgg ctcaaggtca tcgaggcttt
     3781 ccgcaagtcc gggaatagac ccgagtggat gatcctcgac gtgatcccgg tcattccgcc
     3841 ggagctccgg ccgctcgtgc cgctcgaggg cggccggttc gccacctccg atctgaacga
     3901 cctctaccgc agggtcatca acaggaacaa ccggctgcac cgcctcgagg agctcaaggc
     3961 accgtcggtc atcatccgga atgaaaagcg gatgctgcag gaagccgtgg acgcgctttt
     4021 cgacaacggc agacgcggac gggtgcttcg cgggccgaac aagaggccgc tcaagtctct
     4081 ctccgacatg ctcaagggaa aacagggccg gttccgccag aaccttctcg gaaagcgcgt
     4141 ggactattcc ggccgctccg tgatcgtcgt gggcccggac ttgaaactcc accagtgcgg
     4201 ccttccgaag aaaatggcgc tggagctgtt taaacccttc atcttcaaca agcttgagga
     4261 aaagggctat gcaacgacca tcaaaagcgc gaagaagatg atcgaacggg agcggcccga
     4321 ggtatgggac gtgctcgaag aggtgatcca ggagcacccg gtgctcctga accgcgcccc
     4381 gacgctccac cggctcggca tccaggcctt tgaccccgtg ctcgtcgagg gaaaggcgat
     4441 ccgccttcac ccgctcgtgt gcgcggcgtt caacgcggac ttcgacggcg accagatggc
     4501 ggtccacatc ccgctttccg tcgaggcgca gcttgaagcg cgcgtcctca tgctttcggt
     4561 gaacaacatc ctgtcgccgg cgaatggaag accgatcacc gttccgtcac aggacattgt
     4621 gctcggctgt tactatctga ccaaggcacg ctccggcgtt ctgggggagg gcaagatttt
     4681 ctccggccgc gacgaggtgc gcatcgccta cgaccagggc gtggcggaca tccatgcgcg
     4741 gatccgtgtc cgcatcaacg gcaagattga agagaccacc gtagggcggg tccttttcag
     4801 cgatatcctg cctgcgggga tgtcgttctc atcggtcaac aaggaaatga ccaagaagga
     4861 attggccaaa ctgatcgatg agtgctaccg gaagctgggt ctgcgacgca cggtcatcct
     4921 gatcgacgac ctgaaggaga tgggcttcaa atacgccacc cgcgcgggta tttccatctg
     4981 catctcggac atgcacattc cgggcaaaaa gcagaagttc atcgatcagg cgcagaaaga
     5041 agtcatggag atccatcatc aatataccga aggtctcatc acgaacggcg agcgttacaa
     5101 caaggaaatc gacatctggg cgaatgt
//
LOCUS       DASSLD010000685         6918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_246426,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000685 DASSLD010000000
VERSION     DASSLD010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246426"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VFK23_12415"
     CDS             complement(<1..393)
                     /locus_tag="VFK23_12415"
                     /inference="COORDINATES: protein motif:HMM:NF020118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HET7319935.1"
                     /translation="MSQELIHVIDQISKEKGISKEMVIEAVESALVSAAKKKYGAQRI
                     AVQIDPKRGDIVMYAYKKVVQDVANPEEEITLEEAQALYPEAQMEGEVPLQVEFQGFG
                     RIAAQTARQVIVQKVREAERDVVFKEFND"
     gene            complement(504..965)
                     /gene="rimP"
                     /locus_tag="VFK23_12420"
     CDS             complement(504..965)
                     /gene="rimP"
                     /locus_tag="VFK23_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET7319936.1"
                     /translation="MADTAEQIRGIIDPILASRGLSLWDMELQKHGPSWLLRIYIDRE
                     GTGVTLDDCEAVSRDLGAALDVEDVIPHAYTLEVSSPGLDRRLSKPDHFARFTGSAVK
                     VKTYQLINGQKVFEGRLLGLSGDRVKVEIKPGQIVEIPMGDIAKASLEVEF"
     gene            complement(1088..1576)
                     /locus_tag="VFK23_12425"
     CDS             complement(1088..1576)
                     /locus_tag="VFK23_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HET7319937.1"
                     /translation="MKQIDDALLDSLTRKAKESPRKRSHHNLHPDLGDPIQRLCVAIE
                     PGTYIRPHRHAAPDTFEVFMLLRGSAVFLLFDDEGRVTERSVLKARGPVMAVEIPAKA
                     WHAMASLESGTVFFEIKQGPYVQPTRQNVAAWAPAEGDQSSPDFEAWYVTARIGDKSP
                     HS"
     gene            1699..3159
                     /locus_tag="VFK23_12430"
     CDS             1699..3159
                     /locus_tag="VFK23_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET7319938.1"
                     /translation="MTTELKNLFLKKRLADLSLEAFKERQGLKRSLGPASLVLLGVGA
                     VIGAGIFVLTGHAAARIAGPAVTLSFLLAAAACAFAGLCYAEFASMIPVAGSAYTYAY
                     ATLGEFVAWIIGWDLVLEYTVGGIAVAIGWSGYAVSFLRDLGFVVPAAFSAPPFAYDV
                     ETRVWTLTGAVLNMPAALVVLFVTVVLVLGIRESAFLNTLVVAIKLFIILAFIAVGVL
                     FIKPELWHPFVPENKGVFGVFGWSGVLRGAGVIFFAYIGFDAVSTLAQETRDPGKNMP
                     IGILGSLLICTILYVSVSLVLTGVVSYTELDTPAPLAVALDAMKLRVFAPLLKIGAIA
                     GLTSVILVILLGQTRVFYSMGRDRLLPPFAAKVHPRFRTPHVNTMITGAVAATSAALL
                     PINIVGELVSIGTLLAFVIVCSGVLVLRHTHPDAPRAFRAPGGAATPVLGAVTCLYLM
                     SGLPRDTWIRLFVWLVIGLVIYFNYGLKKAGNKGMC"
     gene            complement(3205..4050)
                     /locus_tag="VFK23_12435"
     CDS             complement(3205..4050)
                     /locus_tag="VFK23_12435"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HET7319939.1"
                     /translation="MSYFPIKDSHLKGGLLFSLCVIFYGLLLIPVKSRGDQQPAAPPP
                     PKIKAFQPGEALTYEATWSNAMKMGTAVMEVRAERTSDGREVLLLISTSHTEGFAGSL
                     YPLGDVVQSVFDPEIMQPLSFNLHETQGKRQRRRIIEFDHEHNKVTVTKNEDPIKTFT
                     IPDQVQDNLSSIYYLRTTEDLTVGKTIKFEVFDGEQSVLIEVQVLGREKVKTPAGEFD
                     TIKVTAHKGFLMSEGEITIWLTDDVRKIPVLIKGKIKIGSIVFNLKGLKTGGSPEPKQ
                     TESKN"
     gene            4288..4833
                     /locus_tag="VFK23_12440"
     CDS             4288..4833
                     /locus_tag="VFK23_12440"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET7319940.1"
                     /translation="MFKIFNTRKRSVLIPALAGGITGAGLALLVAPKPGKALRKDLKR
                     LARNTQDQVSEAIDTGKELYGDGKAVVIDAVETGRKTFVQGKKKFDEITHRGNNSLVV
                     PILATGVIGAGVAWLFMTKSGKEFQGDLKRAAGTTADFMSTAVDKSKEIYQEGKTAVS
                     GAVESGKRAFIEGTEKLRRVA"
     gene            complement(4985..5962)
                     /gene="hemB"
                     /locus_tag="VFK23_12445"
     CDS             complement(4985..5962)
                     /gene="hemB"
                     /locus_tag="VFK23_12445"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET7319941.1"
                     /translation="MFQPLYRPRRLRSNENIRRMVRETKLSPDDFIYPLFVTHGKGVR
                     KEIGAMPGNFQQSIDNIVKDCEEVKGLGIPAVILFGIPEHKDEVGSEAYSDEGIVQHA
                     IKAIKHKLPELIVITDVCLCEYTSHGHCGVIRNGVVQNDATLELLAKEAVSHARAGAD
                     MVAPSDMMDGRVGAIRHALDSEGFHDTPIMSYAAKYASSFYGPFREAAESTPQFGDRR
                     SYQMDAPNSREAIREVALDIDEGADIVMVKPALSYLDIIYQVKQQFNLPVAAYNVSGE
                     FSMIKAAARLGWIDGERAMMESLVSMKRAGADMILTYFAKEASALLQKA"
     gene            complement(6068..6445)
                     /locus_tag="VFK23_12450"
     CDS             complement(6068..6445)
                     /locus_tag="VFK23_12450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET7319942.1"
                     /translation="MITISDVAADKIKEILKAEGVPNHGLRVSAAAGGCCGPSYEIMI
                     DDKAGDGDAVIEKNGAKLYVDASTAKTIDGAELGFVSDERGEGFVLGFPNGAPSVGSC
                     GCGGEEPRQGSGGGCGSGGCGCS"
     gene            complement(6535..>6918)
                     /locus_tag="VFK23_12455"
     CDS             complement(6535..>6918)
                     /locus_tag="VFK23_12455"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HET7319943.1"
                     /translation="GLRFLIPRAKVARELIPDKLRELGADVMVATAYENVRPSADLDR
                     VRKLFEEKKIAVVTFTSSSTVHNFVEILGQKEYKFLMNGVSVACIGPVTAKTAEQYGM
                     KIDIMPKEYTIPAFVDAMVEYFKKK"
BASE COUNT         1386 a         2024 c         1883 g         1625 t
ORIGIN      
        1 gtcgttgaat tccttgaaga cgacgtctcg ttcggcctcg cgaactttct ggacaatgac
       61 ctgtcgcgcg gtctgggccg cgatcctgcc gaagccctga aactcgacct ggaggggcac
      121 ctcgccttcc atctgtgcct ctgggtagag cgcctgggcc tcttccagcg tgatctcctc
      181 ctcggggttc gccacatcct gaacgacttt cttgtacgcg tacatgacga tgtctccccg
      241 cttcggatcg atctgcaccg caatgcgttg cgcgccgtat ttcttcttgg cagcgctcac
      301 gagggccgac tcgacggcct cgatcaccat ctccttagaa atgcccttct ccttgcttat
      361 ctggtcgatg acatgaatga gttcctgact cataatcacg ttcctcctga aaatatcttt
      421 ggtaataata aaatgataga gccccgcttt gccggacgaa tttcgcatgc caaaagggtg
      481 cggctaccaa atcctgtttt ttcttaaaat tccacctcaa gcgaggcctt tgcgatatcg
      541 cccattggaa tttccacgat ctgacccggc ttgatttcca ccttcaccct gtcgccggac
      601 aggccgagca gccttccttc aaacaccttc tgtccgttga taagttgata ggttttgacc
      661 ttgaccgcgc tgcccgtaaa tcgggcaaaa tggtccggct ttgacagcct tcggtcgagg
      721 cctggagacg acacctcaag cgtatacgca tgaggtatta cgtcttccac gtcgagcgcc
      781 gcgccgagat ccctgctcac tgcttcgcag tcgtcgagcg tgacgcctgt cccttcccgg
      841 tcgatataga tccggaggag ccacgacggc ccgtgtttct gtaactccat gtcccagagg
      901 gaaagtcccc gtgacgccag aatcgggtcg ataatcccgc gaatctgttc cgccgtatcg
      961 gccatgcttt tcccccaaat aaaaagagtg ggcaaatgaa atgcccactc taaaaaggaa
     1021 cttcccagag tgttgatatt cttttaacac agcccggctg agaatgcaag aaaaaagtgc
     1081 agtctaatca tgaatgcggg gacttatctc caatccttgc cgtcacgtac catgcctcga
     1141 agtccggcga agactggtct ccttccgcag gcgcccaggc tgcgacattc tgccgagtgg
     1201 gctggacgta aggcccctgc ttgatctcga aaaaaactgt cccgctttcg agtgatgcca
     1261 tcgcgtgcca cgccttagcc gggatctcga cggccataac cgggcctcgc gccttgagca
     1321 cggaacgctc ggtcactctt ccttcatcat cgaaaagcag gaacaccgcg cttccgcgaa
     1381 ggagcatgaa gacctcgaag gtgtcgggtg cagcatgccg gtgcggcctg atgtaagtcc
     1441 ccggctcgat ggctacgcac aggcgctgga tgggatcgcc gagatcagga tgaaggttgt
     1501 gatgcgaccg tttcctgggc gactcctttg ctttccgggt gagtgaatcc aggagcgcat
     1561 cgtcaatctg tttcatgatg agggcctttc tttcttcgtt ccggaaacca tacggtccgg
     1621 cgaaaaatag tatacactaa gaatagcgcg gagtgtcaat tatgtgagaa gaacgtcgta
     1681 tcgatattct gaatgacaat gacgaccgag ctaaagaacc tcttcctgaa aaagaggctc
     1741 gccgatctga gcctcgaagc ttttaaagaa cgccagggcc tgaagcgatc tttgggcccc
     1801 gcgagtctcg tgctcctcgg cgtcggggcc gttatcggcg cgggcatctt cgttcttacc
     1861 ggccacgctg ccgcccggat cgcggggcct gccgtcaccc tctccttcct cctcgcagca
     1921 gcggcgtgcg cctttgcagg gctctgttac gctgagttcg cttccatgat ccccgttgcc
     1981 ggaagcgcgt acacctatgc ctatgccacg ctgggcgagt tcgtcgcctg gatcataggc
     2041 tgggacctgg tcctggaata tacggtcggc ggaattgccg tggccatcgg atggtccggg
     2101 tacgcggtga gctttctcag ggacctgggc ttcgtcgtgc ccgcggcgtt ctccgcgccc
     2161 cccttcgcgt acgacgtcga gacgcgggtc tggacgctga ccggcgcggt cctcaacatg
     2221 ccggctgccc tggtcgtcct tttcgtgacc gtcgtcctcg ttctgggaat acgggaatcc
     2281 gcattcctga acactctggt cgttgcaatc aaactgttca tcattctcgc cttcatcgcg
     2341 gtgggcgtcc tcttcatcaa gcccgagctc tggcatccgt tcgtacctga gaacaaaggg
     2401 gtcttcgggg tcttcggctg gagcggcgtt ctccggggcg ccggcgtcat cttctttgcc
     2461 tacatcgggt tcgacgctgt ctcgaccctt gcccaggaaa cgagagatcc gggaaagaac
     2521 atgcccatcg gcatcctggg aagccttctt atctgcacga tcctgtatgt gtccgtctcc
     2581 ctggtgctta ccggcgttgt gtcctacacc gagctggaca cacccgcgcc tcttgcagtg
     2641 gcccttgatg ccatgaaact ccgcgtgttc gctcctcttc tcaagatcgg tgccattgcg
     2701 ggcctgacct ccgtcatcct cgtcatcctc ctcggacaga caagggtttt ctactccatg
     2761 ggcagagacc gcctcctgcc cccgttcgcg gcaaaggtcc atccccgttt tcgtacgcct
     2821 catgtcaata ccatgatcac gggcgcggtt gccgcaacga gcgccgccct cctgccgatc
     2881 aacatcgtgg gcgaactggt gagcatcggc acgctcctcg cctttgtcat cgtctgctcg
     2941 ggagtgcttg ttctccggca cacccatccc gacgcacctc gcgctttcag ggcccccggc
     3001 ggcgcagcga cccccgtcct cggagccgta acctgcctct acctcatgtc gggccttccc
     3061 cgggacacct ggatcaggct cttcgtctgg cttgtcatcg gtctcgtgat ctatttcaac
     3121 tatgggctga aaaaggcggg aaacaaagga atgtgttgac aggtctctat ccaataacta
     3181 taaaaagggt tcgcagaaat atattcaatt tttcgattcc gtttgcttag gttcggggct
     3241 cccaccggtc ttcaacccct tcaggttgaa tacgatggac ccaattttaa tttttccttt
     3301 gataagcacc ggaattttcc tgacatcatc ggtaagccat atggtgatct ccccctcgct
     3361 catgaggaac cccttgtggg ccgtgacctt gatcgtgtcg aactccccgg caggcgtttt
     3421 cactttttcc ctgccaagga cctggacctc gatgagcacg ctctgttcgc cgtcgaagac
     3481 ttcaaacttt atggtctttc cgacggtcaa gtcctccgtc gtccgcaaat aatagatcga
     3541 cgaaaggttg tcctgtacct ggtccggaat agtaaaagtc tttatcggat cttcgttttt
     3601 tgtgacagtg actttgttat gctcgtggtc gaactctatg atgcggcgcc tctgcctctt
     3661 accctgggtc tcatggaggt tgaacgacag gggctgcatg atttccggat cgaaaacgct
     3721 ctgaacaaca tctcccagag ggtagagtga acccgcgaag ccctcggtgt gggatgtcga
     3781 tatgagcagg aggacctccc tgccgtcgct tgtccgttcc gccctgacct ccatgaccgc
     3841 agtccccatt ttcattgcgt tcgaccaggt ggcttcataa gtcagcgctt cccctggttg
     3901 aaaagccttg atcttcggcg gcggaggggc tgcaggctgt tgatcccctc tcgacttaac
     3961 aggaataagc aaaagcccgt aaaagataac gcagagggaa aagagtagcc cgcctttcag
     4021 gtgggagtct tttatcggaa aataagacat ataaatagat tatcaggctc aaatgcaaaa
     4081 gtcaaacagt ccttaaggat tagacatgaa tactgcagag gtcttttgac caagcttttt
     4141 tcctgatatc caccataccg actgcttttt tctctcgagg atgaaacgcg gtgatataat
     4201 ttaactacaa agtcagatca gagacacctt tgatccttga aaagttgctg ttccggaaca
     4261 aattgacaga agaaagaggt gatgtctatg tttaagatat tcaatacgag gaaaagatcg
     4321 gttctgatac cggccctggc gggcggaata accggggcgg gtttggctct cctggtcgcg
     4381 ccgaaaccgg gaaaagcgct ccgcaaggac ctgaaacggc tcgccaggaa cactcaggac
     4441 caggtctctg aagctattga tacaggcaag gaactttatg gtgacgggaa agcggtcgtc
     4501 atcgacgccg tggagacagg aaggaagacc ttcgtccagg gcaagaaaaa attcgatgag
     4561 atcacgcata gggggaacaa ctccctggtg gtcccgatcc ttgcaactgg cgtaatcggt
     4621 gccggcgtcg cgtggctgtt catgactaaa tcgggcaagg aatttcaggg agatctgaag
     4681 cgcgccgcgg gaaccactgc ggacttcatg agtaccgccg tggacaagag caaggaaatc
     4741 taccaggaag gcaagacggc cgtttctgga gccgtggaat cgggcaaaag ggcgtttatc
     4801 gagggaacgg agaaattacg gcgcgtggcg tagcggcatg acgacgcgga aaagcgcccc
     4861 tgtggcgctc gaagtttcgg aaatttatac aggaaaaggg cgcctggctt taagtcaggc
     4921 gccctttctt tattctctcg aagaacaacg gcgcgctgtc cgccggttcc gtctgcccgg
     4981 tctgtcaggc cttctgtaga agggcggacg cctccttggc gaaatacgtg aggatcatgt
     5041 ccgcgcccgc gcgcttcatc gatacgagcg actccatcat ggctcgctcg ccgtcaatcc
     5101 agccgagcct ggcagcggcc ttgatcatcg agaactcgcc gctcacgttg taggccgcga
     5161 cgggcagatt gaactgctgc ttgacctggt agatgatgtc gaggtaggaa agtgcgggct
     5221 tcaccatcac gatgtccgcc ccttcgtcga tgtccagggc gacctcccgg atcgcctcgc
     5281 ggctgttcgg ggcatccatc tggtacgagc gccggtcgcc gaactgggga gtggattccg
     5341 cggcttcgcg gaacgggccg tagaagctcg acgcgtattt cgccgcgtag gacatgatgg
     5401 gggtatcgtg gaagccttcg ctgtcgaggg cgtgacggat ggccccgacc ctgccgtcca
     5461 tcatgtccga gggggccacc atgtccgctc ctgcccgggc gtgcgaaacc gcctcctttg
     5521 ccaggagctc gagcgtggcg tcattctgca cgacgccgtt cctgatcaca ccgcagtgtc
     5581 cgtggctcgt gtactcgcag aggcagacgt cggtgataac gatgagctcg ggcagtttgt
     5641 gcttgatcgc cttgatcgcg tgctggacta tcccctcgtc tgagtacgcc tcgctgccga
     5701 cctcgtcctt gtgctccgga atgccgaaga ggatgacggc ggggatgccg aggcctttca
     5761 cctcttcaca gtccttgacg atgttgtcga tggattgctg gaaatttccg ggcatggccc
     5821 cgatctcttt cctgacaccc ttgccgtgag tgacgaaaag cggatagatg aaatcgtcgg
     5881 gagacagttt cgtctcccgg accatgcgtc gtatgttttc gttggacctg agccttctcg
     5941 gccggtacaa gggctggaac atgagatctc cttctttacg caaagcgcag ggcgcatggc
     6001 gcaaagcgtt aactaaaatc ttaactacct gtcttcttcg ctatgcgcta tgctctatgc
     6061 gatggtgtta actgcacccg cagccgccgc tgccgcaccc gccgccgctt ccctggcgag
     6121 gttcttcacc gccgcagccg cagctgccga cggaaggagc gccgttcggg aagccaagaa
     6181 cgaagccctc gccgcgctcg tcgctcacga acccgagctc cgccccatcg atcgttttgg
     6241 ctgtagacgc atcgacataa agctttgcgc cattcttttc gatgacggca tcgccgtctc
     6301 cggccttgtc gtcgatcatg atctcgtaag aaggcccgca gcacccgccc gctgctgccg
     6361 agacgcgcag gccgtggttc gggacgccct ccgccttgag gatttccttg atcttatccg
     6421 ctgctacatc cgatatggta atcatcaaat ctcctttttt cttctgtttg tcattccggg
     6481 cttgcaagca tgccccggca cgatttaagc aggggcggga atgacagaat atggttactt
     6541 tttcttaaaa tactccacca tcgcgtccac gaacgcgggg atggtgtatt ccttcggcat
     6601 gatgtcgatc ttcatgccgt actgttccgc ggtcttcgca gtcaccggac cgatgcaggc
     6661 gaccgagacc ccgttcatta aaaatttata ctctttttgg ccgagtattt caacaaaatt
     6721 atggaccgtg gaggaactgg tgaacgtcac gacggcgatt tttttctctt caaacagctt
     6781 tctgacgcgg tccaagtccg ctgagggacg cacattctca taggccgtgg ctaccataac
     6841 gtcggccccg agttccctga gtttgtccgg aatgagctca cgggcgactt tcgcacgggg
     6901 gatcaggaac cgcaggcc
//
LOCUS       DASSLD010000686         2967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160
            k141_1461338, whole genome shotgun sequence.
ACCESSION   DASSLD010000686 DASSLD010000000
VERSION     DASSLD010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461338"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFK23_12460"
     CDS             complement(<1..258)
                     /locus_tag="VFK23_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7319944.1"
                     /translation="MAIDVGKNIQIIKDLFSAIGRRDVPAILNCLDENVDWQSPATGT
                     TVKEISWSRPRHGKSEVIAFFNDVREKLTLDEMYYTNILAEG"
     gene            477..2498
                     /locus_tag="VFK23_12465"
     CDS             477..2498
                     /locus_tag="VFK23_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET7319945.1"
                     /translation="MFIFAFTIFLSAFLLFQIQPMIAKMILPWFGGSASVWITSMLFF
                     QTALLFGYLYAHWSVRKLKPWVQAAVHSGLLFVSLFLLPAQPSPAWKPTGHEDPILRI
                     LGLLTVSIGLPYFLLSTTSPLIQAWYARTYKTSLPYKLFGLSNLASLLGLLAYPVLIE
                     PNVTLRLQSLGWSTAFAVFVALGIAAAFLSRRDDADAAYKAEGPPDAAGGDHVPPRLR
                     EQLLWVLLAACASTLLLSITNHLTQNIASIPFLWVLPLSLYLLTFILAFDFERAYNRK
                     VFSWLMTIALAAMAYGLVQWNSRTNLKLVIPTFLSGLFLVCMFCHGELVRRKPAPRHL
                     TSFYLMISIGGALGGLLVGLAAPRVLPAYFELPITLILCSVLLLLVLEYRGSRILPVL
                     SWVAAAAVFAASGYYLTVYGKEARVMARNFYGGLRVTEHDTGTKDEVRMLVHGTVTHG
                     VQYTALNRRRTPISYYGRESGVSLAAKYLRGSTLRVGVIGLGAGSLAAYARHGDVFRF
                     YEINPLVEQMARSEFTYLADCPGKVDVVLGDGRLSLEREPDRSYDLLVIDAFSGDAIP
                     VHLLTKQALELYFRRLEPNGILALHITNSHLDIEPVVGKLASALGKHALLVETDNDEE
                     KKIYRSQWALLSTRPVNDPEILGKARALKDRPDLRLWTDDYNNLFQILK"
     gene            complement(2518..>2967)
                     /locus_tag="VFK23_12470"
     CDS             complement(2518..>2967)
                     /locus_tag="VFK23_12470"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HET7319946.1"
                     /translation="IHMYPKADIPVFEMSLSYTFNEWHPKPLQYHYDLAKELSELRRS
                     GVLIIGSGNIVHNLGMLDFENIDAPVFDWAEEFDEKVKASLESANHAELIHYRNMGPS
                     SLLAVPTLDHYLPMIYAIALREKDEPLAFVHEGFQYGSISMRCFRIG"
BASE COUNT          567 a          919 c          777 g          704 t
ORIGIN      
        1 gccctcggcc aggatgtttg tataatacat ctcatccagg gtgagctttt ccctgacatc
       61 gttgaagaag gcaatcacct cgctcttccc gtgtctcggc ctggaccagg aaatttcctt
      121 caccgtcgtt cccgtcgccg gagactgcca atcgacgttc tcgtcaagac aattcaggat
      181 cgcggggaca tctcttcgtc cgatcgccga aaaaaggtcc tttattatct ggatattttt
      241 ccccacatca attgccatac tctcgcctcc cctttttaca ccatgaagga aacgaactcc
      301 gtcgctccct agcgtacact gccttcgata attcaatgat agaaaacgtt gcatccttct
      361 gtcaatcagc agaaagtagc aacgatcacg gaaccgttgc aaaagggacg acttccgggt
      421 tcccagtcca attttcaggc ctgccacgtc acggttatgt tataatcggc tccgccatgt
      481 tcatctttgc cttcaccata ttcttgagcg cctttctgct cttccagatc cagcccatga
      541 tcgcaaaaat gattttgccc tggttcggcg gatcggcatc ggtctggatc acgtcgatgc
      601 tttttttcca gacagcgttg ctgttcggct atctgtatgc acactggtcg gtccggaaac
      661 tcaagccgtg ggtccaggcg gccgtccatt caggcctgct cttcgtgagc ctttttttgc
      721 ttcctgcgca gccctctccg gcatggaagc cgacggggca cgaagatccg atccttcgca
      781 tcctcggcct tctgacggtc tccatcggcc tgccctactt cctgctctcg acaacgagtc
      841 cgctcatcca ggcatggtat gcgcgcacct ataagacctc attgccgtac aagcttttcg
      901 gtctgtcgaa cctggcatct cttttggggc ttctggctta tccggttttg attgagccga
      961 atgtaaccct ccggctccag tccctcggct ggtcaacggc ctttgcggtt tttgtcgcgc
     1021 tcggcatcgc cgccgccttc ttgagccgca gagatgatgc cgacgcggct tacaaagcag
     1081 aggggccgcc cgacgctgca ggcggagacc acgttcctcc ccgcctgagg gaacaacttc
     1141 tgtgggtttt gctggccgcc tgcgcatcga cgctcctgct ttcgatcacg aaccatctga
     1201 ctcagaacat agcatcgatc ccctttctct gggtcttgcc cctctcattg tatctcctca
     1261 ccttcatttt ggctttcgac ttcgagcggg cctataaccg gaaagttttc tcctggctca
     1321 tgacgatagc gctggcggcc atggcatacg gcctcgtaca atggaattcc cgcaccaacc
     1381 tcaagctggt catcccgacc ttcttgtccg gcctgttcct cgtctgcatg ttctgtcacg
     1441 gtgaactcgt gaggcgcaaa cccgctcccc ggcacctgac ctccttctat ctcatgatct
     1501 ccatcggcgg cgcattgggc ggtctgctcg tggggctcgc agccccccgc gtcctgccgg
     1561 cgtatttcga gctcccgatc acgctcattc tctgctcggt cctcctcctg ctggtcctgg
     1621 agtaccgcgg gtcgcgaatc cttcccgttt tgagctgggt ggcggcggcc gcggtcttcg
     1681 cggcgtctgg atattatctg accgtgtatg ggaaagaggc ccgggtgatg gcgagaaact
     1741 tctacggcgg cctcagggta accgaacatg atacggggac gaaagatgaa gtccgcatgc
     1801 tcgtccatgg caccgtgacg cacggcgtcc agtacacagc gctgaatagg cgccgcacgc
     1861 cgatctctta ctacggccgg gaatcgggcg tatctcttgc cgcgaaatac cttcggggct
     1921 caacgctccg cgtgggagtc atcggtctcg gcgcgggctc acttgccgcc tacgcgaggc
     1981 acggagacgt cttccggttc tacgagatca acccgctcgt ggagcagatg gcgcggagtg
     2041 aattcaccta tcttgcggac tgtcccggca aggtcgatgt cgtcctgggc gacggccggc
     2101 tgtctctcga acgcgaaccc gacaggtcgt acgatctcct ggtcatcgac gccttctccg
     2161 gcgacgccat tcccgtccac ctgctgacaa agcaggccct ggagctctac ttccgccgcc
     2221 tggagcccaa tggcatcctg gctctccata tcacgaattc ccacctggac atagaacccg
     2281 tcgtcgggaa gctcgcctca gcgctcggaa agcacgcgct cctcgttgaa acggacaacg
     2341 atgaagagaa aaaaatctat cgttcccaat gggcgctcct gtcgacccgt cccgtcaatg
     2401 atccggaaat cctgggaaaa gcccgggccc ttaaagacag accggacctt cgcctgtgga
     2461 ccgacgacta caacaatctg ttccagatat taaaatagga gggggggaca ccgcaggtca
     2521 tccgattcgg aaacacctca tcgagatcga tccgtattga aaaccctcat ggacgaacgc
     2581 gaggggttcg tccttctccc tgagcgctat ggcataaatc atgggaagat aatggtcgag
     2641 ggtcggcacc gccaggagcg aggaggggcc catgttccgg tagtggatca gttccgcgtg
     2701 gtttgcgctt tccaggctgg ccttcacctt ttcgtcgaac tcctcagccc agtcaaagac
     2761 cggagcgtcg atgttttcaa aatccagcat gcccaaattg tggacgatgt tgccgctgcc
     2821 gatgatcaag acaccgctcc ggcgcagttc ggaaagttct ttcgccagat cgtaatggta
     2881 ctggagcggt ttggggtgcc attcgttgaa cgtataggaa aggctcatct cgaagacggg
     2941 gatgtccgcc ttgggataca tgtgtat
//
LOCUS       DASSLD010000687         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Nitrospirota bacterium isolate SMAG_U1160 k141_115397,
            whole genome shotgun sequence.
ACCESSION   DASSLD010000687 DASSLD010000000
VERSION     DASSLD010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963319
            Sequence Read Archive: SRR25158196
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirota bacterium (soil metagenome)
  ORGANISM  Nitrospirota bacterium
            Bacteria; Nitrospirota.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 05:35:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,496
            CDSs (total)                      :: 2,475
            Genes (coding)                    :: 2,457
            CDSs (with protein)               :: 2,457
            Genes (RNA)                       :: 21
            tRNAs                             :: 18
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 0 of 18
            Pseudo Genes (incomplete)         :: 11 of 18
            Pseudo Genes (internal stop)      :: 7 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Nitrospirota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115397"
                     /isolate="SMAG_U1160"
                     /isolation_source="soil"
                     /db_xref="taxon:2026887"
                     /environmental_sample
                     /country="China"
                     /lat_lon="32.32416667 N 113.6247222 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..987)
                     /locus_tag="VFK23_12475"
     CDS             complement(235..987)
                     /locus_tag="VFK23_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7319947.1"
                     /translation="MIKLIDLNKTYGKLTAVNGINLEVAEGEVFGFLGPNGAGKTTTI
                     RIMAGLLQPTSGSVLIGGHDVQKEPLKAKFITGFIPDRPYLYEKLTATEFMHFVARLY
                     EMRDSKNRIAELLDIFGLPDWKDELIENFSHGMKQRLVMASALLHHPRVLVVDEPMVG
                     LDPRGARLVKDIFRDLASNGVTVFMSTHTLEIVEQMCTRVAIINKGDIIAEGSVEDLG
                     RMARMQGSHLEPIFLKLTGGDEVLQEGIFGLK"
     gene            complement(1095..>2536)
                     /locus_tag="VFK23_12480"
     CDS             complement(1095..>2536)
                     /locus_tag="VFK23_12480"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HET7319948.1"
                     /translation="IISVLILLFWVTMLGLLVERTYLRPSAVIALDAATEEGVRATDE
                     WFGIYQKGAKIGYAYTSIRPEAETYHLREESELDILALGSLQRVKTKINSYTTRNFLL
                     KYFDFSLQSEATSMKIKGAGVGKKLIVDIMTGGQTRTERIPLASPPYLSVNIKPALLL
                     AGLEPGKTYRFPIFNPATMSTEDADITVDSKERIKVGSAEQNVYKLRESFQGMETTSW
                     VNQDGETLKEESALGYILVKEPMAEARKRDKGGPVIDIISLTMIPSDPIKEPSKVHYL
                     RARLKGVPLTGFPLDGDRQTLKGDVLEVLVDNAPGNYLLPHSGGELAGYLQPNALIQS
                     DDKKIIDQTVKILAGEKNAREAAKKLNTWVYTVIDKKPVVSIPSALEVLKQREGDCNE
                     HTALYTALARAAGIPTRMAAGIVYLENGFYYHAWPEVWLGAWVAVDPTFDQFPADATH
                     IRFVTGNLDRQSDIVRLVGKLKVEVLEYR"
BASE COUNT          485 a          710 c          732 g          609 t
ORIGIN      
        1 gacacggtac gacatcccgt agagaaaggc ccagaatgcg aggccgagga gcgtgaggac
       61 agccgtcttg accccgtcgc ccggagcgcg acgggtgagg cggtttttta tggcagtata
      121 gcgcggggta aggagcagga gcataggtct taaacatcaa aacatcaaat cgttgctagc
      181 atgaacaatt gtatgtgcga ttcaatttgt ttccgggcct gctgctgccc gttttcattt
      241 caaaccgaaa attccttcct gcagaacttc atcccctccg gtcagcttca ggaatatggg
      301 ctccaggtgg cttccctgca tgcgcgccat gcgtcccagg tcctcgacgc tgccctccgc
      361 gatgatgtcg cccttgttga tgatggccac tcgcgtgcac atctgctcga cgatctcgag
      421 cgtatgcgtg gacatgaaca ccgttacgcc gttcgacgcg aggtcccgga aaatatcctt
      481 taccagccgg gcaccgcgcg gatccagacc gaccatgggc tcgtccacga cgaggaccct
      541 ggggtgatgg agcagggcgg aggccatgac cagtcgctgt ttcatgccgt gcgaaaagtt
      601 ttcgatgagc tcatccttcc agtcagggag gccgaaaata tccaggagtt cggcgatccg
      661 attttttgaa tcgcgcatct cgtacagcct tgccacgaag tgcatgaatt ccgttgccgt
      721 cagtttttca tacagataag gccggtccgg gatgaatccg gttatgaatt tggccttgag
      781 cggttctttc tggacgtcgt ggcctccgat gagcacgctt ccgctcgtgg gctgcagcag
      841 tccggccatg atcctgatgg tcgttgtctt gcccgcgccg ttcggtccga gaaatccgaa
      901 cacctctccc tcggcgactt cgaggttgat gccgttcacc gcggttagtt tgccgtatgt
      961 tttattaagg tcgatgagct tgatcatatg cctttcatga acaccgatga accgcggctc
     1021 tcctgtgggt catggccgct gtgactgcgc gcgccgtttt gggtgcaaag gccgcggcca
     1081 taaacgagcg caggtcaacg gtattccagg acttccacct tcaattttcc gaccagcctt
     1141 acaatatccg attgccggtc gagatttccg gtcacgaacc tgatgtgggt ggcatctgcg
     1201 gggaactggt cgaaagtcgg atcgaccgct acccacgccc cgagccagac ttcgggccac
     1261 gcatgatagt agaatccgtt ttccaggtat acaatccctg ccgccatgcg cgtcgggatg
     1321 cccgccgccc gggcaagggc agtgtagaga gccgtatgct cgttgcagtc gccttcgcgc
     1381 tgtttgagga cctccagggc gctggggatg cttaccaccg gcttcttatc gatcaccgtg
     1441 tatacccagg tattcaattt cttcgcggct tcccgggcgt ttttctctcc ggcgaggatc
     1501 ttaacggtct ggtcgatgat cttcttgtcg tcgctctgta tcagggcatt cggctgcagg
     1561 tagcctgcca attccccgcc ggaatggggc agaagataat ttccgggggc gttatccacg
     1621 aggacctcaa ggacatcgcc tttcagcgtc tgccggtctc catccagggg gaagccggtc
     1681 agggggacgc ctttcaatcg ggcccggagg tagtgcacct tcgacggttc cttgatggga
     1741 tcggaaggga tcatggtgag cgatatgata tcgatgaccg gaccgccctt gtcccgtttc
     1801 cttgcctcgg ccatcggctc cttcaccagg atgtaaccga gagcgctctc ttccttgagg
     1861 gtttcaccgt cctgattgac ccacgacgtg gtctccatgc cctggaagga ctccctcagt
     1921 ttataaacgt tctgttctgc tgatccgacc ttgatgcgct ccttggaatc cacggtgata
     1981 tcggcatcct cggtgctcat ggttgcggga ttgaaaatgg gaaagcggta ggttttcccc
     2041 ggttccaggc ctgcgagcaa gagggcaggt tttatgttca cggacaggta ggggggagag
     2101 gcgagcggaa tacgctccgt acgggtctgg cctccggtca tgatgtccac gatcaatttt
     2161 ttgccgaccc cggcgccctt gatcttcatc gaggtcgcct cggactgcag ggaaaaatcg
     2221 aagtacttga ggagaaagtt cctggtcgta tagctgttga tcttcgtttt tacccgctgg
     2281 agggaaccga gtgcaaggat gtcaagttcc gattcctccc tgagatggta ggtctctgcc
     2341 tcgggtctga tactcgtgta tgcatagcct atctttgccc ccttttgata gatcccgaac
     2401 cactcatctg tcgcgcggac gccttcttct gttgccgcgt cgagggcaat tacggcagag
     2461 ggacggagat acgtgcgctc caccagaaga ccgagcatgg tgacccagaa gagcaggatg
     2521 agcacgctga tgattt
//
